LOCUS       DAJVTY010000001       107627 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVTY010000001 DAJVTY010000000
VERSION     DAJVTY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 107627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107627
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.001"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OQQ66_000001"
                     /pseudo
     CDS             complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OQQ66_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OQQ66_000002"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OQQ66_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1903076.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OQQ66_000003"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OQQ66_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1903077.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="OQQ66_000004"
     CDS             complement(2414..3343)
                     /locus_tag="OQQ66_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1903078.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="OQQ66_000005"
     CDS             complement(3389..4213)
                     /locus_tag="OQQ66_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1903079.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OQQ66_000006"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OQQ66_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OQQ66_000007"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OQQ66_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1903080.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="OQQ66_000008"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="OQQ66_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1903081.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5107..5230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="OQQ66_000009"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="OQQ66_000009"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1903082.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="OQQ66_000010"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="OQQ66_000010"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1903083.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="OQQ66_000011"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="OQQ66_000011"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1903084.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKHPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="OQQ66_000012"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="OQQ66_000012"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1903085.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFLQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="OQQ66_000013"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="OQQ66_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1903086.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="OQQ66_000014"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="OQQ66_000014"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1903087.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="OQQ66_000015"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="OQQ66_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1903088.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /locus_tag="OQQ66_000016"
     CDS             11699..12310
                     /locus_tag="OQQ66_000016"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1903089.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OQQ66_000017"
     CDS             complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OQQ66_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1903090.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDFLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQTSLIGRYSTA
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13531..13623)
                     /locus_tag="OQQ66_000018"
                     /pseudo
     CDS             complement(13531..13623)
                     /locus_tag="OQQ66_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13793..14797
                     /locus_tag="OQQ66_000019"
     CDS             13793..14797
                     /locus_tag="OQQ66_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCS1903091.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OQQ66_000020"
     CDS             complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OQQ66_000020"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS1903092.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPILFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OQQ66_000021"
     CDS             complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OQQ66_000021"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1903093.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGNKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            complement(17753..18859)
                     /gene="glf"
                     /locus_tag="OQQ66_000022"
     CDS             complement(17753..18859)
                     /gene="glf"
                     /locus_tag="OQQ66_000022"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HCS1903094.1"
                     /translation="MQYEFIIIGSGLFGAVCAYELNKRNKRVLVIEKRDHIGGNIYTE
                     KVDDIHIHKYGAHIFHTNDQYIWNYINKFASFNRFTNSPLAYHKGKLYNLPFNMNTFY
                     QLWGTKTPSEAQDKIDLQRSKYSNITPTNLEEQALSLVGEDVYEKLIKGYTEKQWGRS
                     CSELPPFIIKRIPVRFTFDNNYFTDRFQGVPIGGYTQIIDKMLNGVDLLLNVDYLSEK
                     DKYNSMAERIIYTGPIDAYFDKKLGTLEYRSLKFITKKIEEANYQGNAVINYTEKDIP
                     FTRIIEHKHFDDSYETEYTFITEEYPIEWSEGQEPYYPINDKKNMDIYHKYRELSQME
                     SKVIFGGRLAEYKYYDMHQVIRSALNCVNKILHD"
     gene            complement(18892..19422)
                     /gene="rfbC"
                     /locus_tag="OQQ66_000023"
     CDS             complement(18892..19422)
                     /gene="rfbC"
                     /locus_tag="OQQ66_000023"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011093025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1903095.1"
                     /translation="MKVQDTVLDGVKIIEPVVYGDNRGFFLEVYQKQRYQNLLNIEFD
                     FVQDNYSRSSKNVLRGLHFQKTKPQGKLVRVVRGEVFDVAVDIRKESPNYGQWIGVLL
                     SEMNKKQFWVPPGFAHGFVVLSEMADFEYKCTEYYDPEDECCLLWNDPELNIQWPLSN
                     PILSDKDMKGCLLKEL"
     gene            complement(19437..20606)
                     /locus_tag="OQQ66_000024"
     CDS             complement(19437..20606)
                     /locus_tag="OQQ66_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1903096.1"
                     /translation="MDCLDEKDHLRDNHSLKIVLTANSSWYIYNFRRSTIKALINIGF
                     DVLVVVPDYEYKDSILKFGAKFEKVDLKPKSINIFTECTSLISYYKIIKKFNPQVILT
                     FTPKANIYCGLLAYKFDAKVIPNISGLGSAFVNSNTILSQIVKLLYKIALKKAAFVFF
                     QNEDDRQLLINSGCVNYEKTCRIFGSGVDLSKFLPSNKNLKQRSCVKFILVARLLYTK
                     GILHYLKAAEIIKSKYPNCSFSLLGAFEKNEKIITKELIEGFCTRGIVNYYGTSDDVA
                     SIMKHYDAIVLPSFYREGVPKALIEGASSGLAILTTDNVGCRDMVQNEVNGFLCQPND
                     LDSLVDIIEKYILLTDEMKNTMSIKSREFAESHCDENKIIEKYTNTINDVLNRNH"
     gene            complement(20569..21441)
                     /locus_tag="OQQ66_000025"
     CDS             complement(20569..21441)
                     /locus_tag="OQQ66_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCS1903097.1"
                     /translation="MNRVAVIIVLYYPDVSKINKMIDSLGKKQCEILLVDNTPELHLE
                     SDVLCNNNIHYYHLGDNKGIAFAQNYGFIKAIGLGKSLFFTFDQDSCISKDYISSMLK
                     EYEYACSLRSNIAALGPTIINERNGKKYDREIKLGSQISETIHDVESIISSGALIPLE
                     AIINIGLNKVSWFIDLIDIEWSFRARKEGWNILVTDKVLISHNIGSKDINIMGIKTFT
                     VCSPFRLYYVYRNWLLALREPVFPLRYKLKKLITMPVRIIIYAFCERGGQRLNYIFKG
                     IKDGLLGRKGSFKG"
     gene            complement(22244..22876)
                     /locus_tag="OQQ66_000026"
     CDS             complement(22244..22876)
                     /locus_tag="OQQ66_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCS1903098.1"
                     /translation="MLEHIVVFDVCGTLYLSNTTFDYVVFVHRKQKKYTKLIKCLLLR
                     SLFGKLLHKLRICSLRKHFLKTLEGYEKSTLEELANLFYDEFLIFKEKNEVMALLERY
                     KHEKIILMSASVDPVINVIAKNLNVEAVSSKLEFIDNKSTGLLEYNLTGIKHLQVNNP
                     VSLIVTDNFSDINLIKLADTAVIISKAKDKNKWRVLLNRFGVSKDKVRFM"
     gene            complement(22878..24059)
                     /locus_tag="OQQ66_000027"
     CDS             complement(22878..24059)
                     /locus_tag="OQQ66_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HCS1903099.1"
                     /translation="MTIFIAIIMLMYSGLLLSINKDIKSPSAILFFIWGGLLFLSGVN
                     GDITNYTLCIILFSCLSFSVGALLAKPYAPIFEHVFSYNINNTKALVLITNIISFVIL
                     IYTLIVFLYYYKGGFTESYINSRTEINYGDKSGLIKIYGYIYYLLYPLVYVWSFLYFK
                     NKYKKIEEGERARLPYNNRTFYFVFFTSLFYALLSTAKIKVLLLIIPIVFLRLFFSKI
                     SLKYLLVTVSGIIFFFYLSMLFLNKIDDSSGAFEALKYGLMNYSLANIFALDSVLQGK
                     AVVIDCNGDATCGLANFISYKEYKTNVFTIFYSLTKYSDFLYSLIFFFVIGFFHSSLY
                     NVAKKNKKTISVVICSILYFPLLFQFFDQLYMLMFYIYAIAMLYVICFCSRLTLTLKG
                     V"
     gene            complement(24062..24826)
                     /locus_tag="OQQ66_000028"
     CDS             complement(24062..24826)
                     /locus_tag="OQQ66_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HCS1903100.1"
                     /translation="MALFIACHKQTELPNHECYIPIHAGKKISGKMLDQVLGDDTGNN
                     ISCKNKNYCELTVIYWLWKNKLFNDDYIGLVHYRRYFGNVLSNFKFKEVRIYDRANIS
                     NKMKQYDIIIPVKESLKLSVVEHYRKFHNVEDLMLAKKIVDKLYPDYSVAFRELLEQK
                     KISLYNMFIMKAGIFDCYCDWLFSILDELEKEINLCRYDEYQSRVFGFIAERLLNVWI
                     NKNKEVFKVGEEFVINTDECSGRGAQLISQLRAKLK"
     gene            complement(24839..26086)
                     /locus_tag="OQQ66_000029"
     CDS             complement(24839..26086)
                     /locus_tag="OQQ66_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050009647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HCS1903101.1"
                     /translation="MKLVTSLNKNIFYLAILQGSYFILPLLTFPYLVRVLGPYSFGLL
                     GFCQATMQYLVMVTDYGFNWSATQDVSKSKNDKEKLTTIFWNVFFSKIILALSAIVIF
                     FGITFFIKELYYAQVVLISFIPMVLGNILYPVWFFQGLEKMKWITLCSLSARLLLIPL
                     TFLLVKNPQDIWLAALIQGGVNFIAGLIGFAIIINNNWIGKLVFDYDGIIYSLKSGWH
                     IFISTAAISLYTTTTVVVLGFICGPVSVGYFNAANTIRNAAQSLMNPIYQAVYPRINS
                     LFEENYAKSMNLLKKSFKGVLFISFSGSLLLCILSPIIIRYGVGDKYTESISILRILA
                     FLPFIISLSNFFGIQTMLTHGYKRQFSKILLICGVMHVIIIAPLIFFAGAYGAAMTVL
                     ITETVVASLMYLFLKRKKINLFK"
     gene            complement(26129..27013)
                     /gene="rfbA"
                     /locus_tag="OQQ66_000030"
     CDS             complement(26129..27013)
                     /gene="rfbA"
                     /locus_tag="OQQ66_000030"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1903102.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVDKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKILAEPL
                     KKNAYGQYLLNIINNKIS"
     gene            complement(27071..27970)
                     /gene="rfbD"
                     /locus_tag="OQQ66_000031"
     CDS             complement(27071..27970)
                     /gene="rfbD"
                     /locus_tag="OQQ66_000031"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1903103.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGMYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPATAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(27970..29055)
                     /gene="rfbB"
                     /locus_tag="OQQ66_000032"
     CDS             complement(27970..29055)
                     /gene="rfbB"
                     /locus_tag="OQQ66_000032"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1903104.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(29427..30320)
                     /gene="galF"
                     /locus_tag="OQQ66_000033"
     CDS             complement(29427..30320)
                     /gene="galF"
                     /locus_tag="OQQ66_000033"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1903105.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(30495..31889)
                     /gene="wcaM"
                     /locus_tag="OQQ66_000034"
     CDS             complement(30495..31889)
                     /gene="wcaM"
                     /locus_tag="OQQ66_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1903106.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKQVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSCVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERGE"
     gene            complement(31900..33120)
                     /gene="wcaL"
                     /locus_tag="OQQ66_000035"
     CDS             complement(31900..33120)
                     /gene="wcaL"
                     /locus_tag="OQQ66_000035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1903107.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPQGKVAKLRYRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELDVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISELWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(33117..34397)
                     /gene="wcaK"
                     /locus_tag="OQQ66_000036"
     CDS             complement(33117..34397)
                     /gene="wcaK"
                     /locus_tag="OQQ66_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1903108.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNQHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVENGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQEAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMRMVQSVLERIGEAK"
     gene            complement(34565..36043)
                     /gene="wzxC"
                     /locus_tag="OQQ66_000037"
     CDS             complement(34565..36043)
                     /gene="wzxC"
                     /locus_tag="OQQ66_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1903109.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIIAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(36045..37439)
                     /gene="wcaJ"
                     /locus_tag="OQQ66_000038"
     CDS             complement(36045..37439)
                     /gene="wcaJ"
                     /locus_tag="OQQ66_000038"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1903110.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(37494..38864)
                     /gene="cpsG"
                     /locus_tag="OQQ66_000039"
                     /gene_synonym="manB"
     CDS             complement(37494..38864)
                     /gene="cpsG"
                     /locus_tag="OQQ66_000039"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1903111.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALSVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(39057..40493)
                     /gene="cpsB"
                     /locus_tag="OQQ66_000040"
     CDS             complement(39057..40493)
                     /gene="cpsB"
                     /locus_tag="OQQ66_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1903112.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEQAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40496..41719)
                     /gene="wcaI"
                     /locus_tag="OQQ66_000041"
     CDS             complement(40496..41719)
                     /gene="wcaI"
                     /locus_tag="OQQ66_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1903113.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41716..42195)
                     /gene="gmm"
                     /locus_tag="OQQ66_000042"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41716..42195)
                     /gene="gmm"
                     /locus_tag="OQQ66_000042"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1903114.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLMPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(42195..43163)
                     /gene="fcl"
                     /locus_tag="OQQ66_000043"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(42195..43163)
                     /gene="fcl"
                     /locus_tag="OQQ66_000043"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1903115.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(43166..44287)
                     /gene="gmd"
                     /locus_tag="OQQ66_000044"
     CDS             complement(43166..44287)
                     /gene="gmd"
                     /locus_tag="OQQ66_000044"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1903116.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(44314..44862)
                     /gene="wcaF"
                     /locus_tag="OQQ66_000045"
     CDS             complement(44314..44862)
                     /gene="wcaF"
                     /locus_tag="OQQ66_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1903117.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44878..45624)
                     /gene="wcaE"
                     /locus_tag="OQQ66_000046"
     CDS             complement(44878..45624)
                     /gene="wcaE"
                     /locus_tag="OQQ66_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1903118.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45635..46852)
                     /gene="wcaD"
                     /locus_tag="OQQ66_000047"
     CDS             complement(45635..46852)
                     /gene="wcaD"
                     /locus_tag="OQQ66_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1903119.1"
                     /translation="MSTPIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAVVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46827..48044)
                     /gene="wcaC"
                     /locus_tag="OQQ66_000048"
     CDS             complement(46827..48044)
                     /gene="wcaC"
                     /locus_tag="OQQ66_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1903120.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTVVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNSYQNL"
     gene            complement(48041..48529)
                     /gene="wcaB"
                     /locus_tag="OQQ66_000049"
     CDS             complement(48041..48529)
                     /gene="wcaB"
                     /locus_tag="OQQ66_000049"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1903121.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48532..49371)
                     /gene="wcaA"
                     /locus_tag="OQQ66_000050"
     CDS             complement(48532..49371)
                     /gene="wcaA"
                     /locus_tag="OQQ66_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1903122.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(49549..51711)
                     /gene="wzc"
                     /locus_tag="OQQ66_000051"
     CDS             complement(49549..51711)
                     /gene="wzc"
                     /locus_tag="OQQ66_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1903123.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKELIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAMGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51714..52157)
                     /gene="wzb"
                     /locus_tag="OQQ66_000052"
     CDS             complement(51714..52157)
                     /gene="wzb"
                     /locus_tag="OQQ66_000052"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1903124.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISIAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(52163..53302)
                     /gene="wza"
                     /locus_tag="OQQ66_000053"
     CDS             complement(52163..53302)
                     /gene="wza"
                     /locus_tag="OQQ66_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1903125.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53611..53760
                     /locus_tag="OQQ66_000054"
     CDS             53611..53760
                     /locus_tag="OQQ66_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903126.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            53961..55544
                     /locus_tag="OQQ66_000055"
     CDS             53961..55544
                     /locus_tag="OQQ66_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1903127.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(55818..57671)
                     /gene="asmA"
                     /locus_tag="OQQ66_000056"
     CDS             complement(55818..57671)
                     /gene="asmA"
                     /locus_tag="OQQ66_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1903128.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(57693..58274)
                     /gene="dcd"
                     /locus_tag="OQQ66_000057"
     CDS             complement(57693..58274)
                     /gene="dcd"
                     /locus_tag="OQQ66_000057"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1903129.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(58366..59007)
                     /gene="udk"
                     /locus_tag="OQQ66_000058"
     CDS             complement(58366..59007)
                     /gene="udk"
                     /locus_tag="OQQ66_000058"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1903130.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            59325..62622
                     /locus_tag="OQQ66_000059"
                     /pseudo
     CDS             59325..62622
                     /locus_tag="OQQ66_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(62661..63518)
                     /gene="alkA"
                     /locus_tag="OQQ66_000060"
     CDS             complement(62661..63518)
                     /gene="alkA"
                     /locus_tag="OQQ66_000060"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1903131.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            63652..65004
                     /gene="yegD"
                     /locus_tag="OQQ66_000061"
     CDS             63652..65004
                     /gene="yegD"
                     /locus_tag="OQQ66_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1903132.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGISIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(65016..66938)
                     /gene="yegI"
                     /locus_tag="OQQ66_000062"
     CDS             complement(65016..66938)
                     /gene="yegI"
                     /locus_tag="OQQ66_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS1903133.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(66935..67696)
                     /gene="pphC"
                     /locus_tag="OQQ66_000063"
     CDS             complement(66935..67696)
                     /gene="pphC"
                     /locus_tag="OQQ66_000063"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS1903134.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(67693..68352)
                     /locus_tag="OQQ66_000064"
     CDS             complement(67693..68352)
                     /locus_tag="OQQ66_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1903135.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(68573..68632)
                     /gene="ibsA"
                     /locus_tag="OQQ66_000065"
     CDS             complement(68573..68632)
                     /gene="ibsA"
                     /locus_tag="OQQ66_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS1903136.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(68905..68961)
                     /locus_tag="OQQ66_000066"
     CDS             complement(68905..68961)
                     /locus_tag="OQQ66_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1903137.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(69233..69289)
                     /locus_tag="OQQ66_000067"
     CDS             complement(69233..69289)
                     /locus_tag="OQQ66_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1903138.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            69568..70815
                     /gene="mdtA"
                     /locus_tag="OQQ66_000068"
     CDS             69568..70815
                     /gene="mdtA"
                     /locus_tag="OQQ66_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1903139.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            70815..73937
                     /gene="mdtB"
                     /locus_tag="OQQ66_000069"
     CDS             70815..73937
                     /gene="mdtB"
                     /locus_tag="OQQ66_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS1903140.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            73938..77015
                     /gene="mdtC"
                     /locus_tag="OQQ66_000070"
     CDS             73938..77015
                     /gene="mdtC"
                     /locus_tag="OQQ66_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1903141.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVEPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            77016..78432
                     /locus_tag="OQQ66_000071"
                     /pseudo
     CDS             77016..78432
                     /locus_tag="OQQ66_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            78429..79832
                     /gene="baeS"
                     /locus_tag="OQQ66_000072"
     CDS             78429..79832
                     /gene="baeS"
                     /locus_tag="OQQ66_000072"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1903142.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            79829..80551
                     /gene="baeR"
                     /locus_tag="OQQ66_000073"
     CDS             79829..80551
                     /gene="baeR"
                     /locus_tag="OQQ66_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1903143.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            80742..81074
                     /locus_tag="OQQ66_000074"
     CDS             80742..81074
                     /locus_tag="OQQ66_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1903144.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            81197..82597
                     /locus_tag="OQQ66_000075"
     CDS             81197..82597
                     /locus_tag="OQQ66_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCS1903145.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            82918..84279
                     /gene="yegQ"
                     /locus_tag="OQQ66_000076"
     CDS             82918..84279
                     /gene="yegQ"
                     /locus_tag="OQQ66_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1903146.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(84609..84926)
                     /locus_tag="OQQ66_000077"
     CDS             complement(84609..84926)
                     /locus_tag="OQQ66_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903147.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            85340..86238
                     /gene="yegS"
                     /locus_tag="OQQ66_000078"
                     /pseudo
     CDS             85340..86238
                     /gene="yegS"
                     /locus_tag="OQQ66_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(86320..87093)
                     /locus_tag="OQQ66_000079"
     CDS             complement(86320..87093)
                     /locus_tag="OQQ66_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1903148.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(87199..88239)
                     /gene="gatD"
                     /locus_tag="OQQ66_000080"
     CDS             complement(87199..88239)
                     /gene="gatD"
                     /locus_tag="OQQ66_000080"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1903149.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(88287..89642)
                     /locus_tag="OQQ66_000081"
     CDS             complement(88287..89642)
                     /locus_tag="OQQ66_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1903150.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(89646..89930)
                     /gene="gatB"
                     /locus_tag="OQQ66_000082"
     CDS             complement(89646..89930)
                     /gene="gatB"
                     /locus_tag="OQQ66_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1903151.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(89961..90413)
                     /gene="gatA"
                     /locus_tag="OQQ66_000083"
     CDS             complement(89961..90413)
                     /gene="gatA"
                     /locus_tag="OQQ66_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1903152.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(90483..91685)
                     /gene="gatZ"
                     /locus_tag="OQQ66_000084"
     CDS             complement(90483..91685)
                     /gene="gatZ"
                     /locus_tag="OQQ66_000084"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1903153.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPH"
     gene            complement(91714..92568)
                     /gene="gatY"
                     /locus_tag="OQQ66_000085"
     CDS             complement(91714..92568)
                     /gene="gatY"
                     /locus_tag="OQQ66_000085"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1903154.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(92876..93928)
                     /gene="fbaB"
                     /locus_tag="OQQ66_000086"
     CDS             complement(92876..93928)
                     /gene="fbaB"
                     /locus_tag="OQQ66_000086"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1903155.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            94185..95462
                     /locus_tag="OQQ66_000087"
     CDS             94185..95462
                     /locus_tag="OQQ66_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1903156.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMSTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            95459..96463
                     /locus_tag="OQQ66_000088"
     CDS             95459..96463
                     /locus_tag="OQQ66_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS1903157.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            96460..97425
                     /locus_tag="OQQ66_000089"
     CDS             96460..97425
                     /locus_tag="OQQ66_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1903158.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(97399..98145)
                     /locus_tag="OQQ66_000090"
     CDS             complement(97399..98145)
                     /locus_tag="OQQ66_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1903159.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFCSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(98197..99015)
                     /locus_tag="OQQ66_000091"
     CDS             complement(98197..99015)
                     /locus_tag="OQQ66_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1903160.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(99080..99880)
                     /gene="thiD"
                     /locus_tag="OQQ66_000092"
     CDS             complement(99080..99880)
                     /gene="thiD"
                     /locus_tag="OQQ66_000092"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1903161.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(99877..100665)
                     /gene="thiM"
                     /locus_tag="OQQ66_000093"
     CDS             complement(99877..100665)
                     /gene="thiM"
                     /locus_tag="OQQ66_000093"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1903162.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(100700..100796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(100888..101160)
                     /gene="rcnR"
                     /locus_tag="OQQ66_000094"
     CDS             complement(100888..101160)
                     /gene="rcnR"
                     /locus_tag="OQQ66_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1903163.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            101281..102105
                     /gene="rcnA"
                     /locus_tag="OQQ66_000095"
     CDS             101281..102105
                     /gene="rcnA"
                     /locus_tag="OQQ66_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS1903164.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            102324..102662
                     /gene="rcnB"
                     /locus_tag="OQQ66_000096"
     CDS             102324..102662
                     /gene="rcnB"
                     /locus_tag="OQQ66_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1903165.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(102744..103778)
                     /locus_tag="OQQ66_000097"
     CDS             complement(102744..103778)
                     /locus_tag="OQQ66_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCS1903166.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLEGIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(103794..106274)
                     /locus_tag="OQQ66_000098"
     CDS             complement(103794..106274)
                     /locus_tag="OQQ66_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1903167.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSEFVQVGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(106290..106964)
                     /locus_tag="OQQ66_000099"
     CDS             complement(106290..106964)
                     /locus_tag="OQQ66_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1903168.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(107044..107586)
                     /locus_tag="OQQ66_000100"
     CDS             complement(107044..107586)
                     /locus_tag="OQQ66_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1903169.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT        27253 a        27385 c        25449 g        27540 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctcccca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 cacccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgataaac
     8641 tcgcctttga accgggcgtg atcccggagc tgctgaagct gcaaaaagcg ggctacaagc
     8701 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcctgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggaacg ttatctggct gagcaagcga tggatcgcgc taacagttat gtgattggcg
     8941 atcgcgcgac cgacattcaa ctggcggaaa acatgggtat taatggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaactcac taaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaaa ttgacgtcca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     9181 cccacggcgg tttccgtatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc cttggcgaca
     9301 aacgcggtat ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt gcccgctgtg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgaatttacc taccagcgcg
     9421 tcggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctgtct tacaccatgg
     9481 gcgtgacgct acacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcctt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgac accctgccct
     9601 cgtcgaaagg agtgctgtga tgaacgtggt gatcctggat accggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgta ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg aactgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 gggcatctgc ttagggatgc aactgcttgg gcggcgcagc gaagagagca acggcgtcga
     9901 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgcccctgcc
     9961 gcatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agatggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttt cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgcagcgc agggtgccga agtgctgcat ctggtggatc tgaccggagc aaaagatccg
    10381 gcaaaacgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt tcctgtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg ttggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtactggcg ctggatgtcc gtattgacga gcaaggtaac
    10621 aagcaggtgg cggtgagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctttgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt cctccggcgg cattggcgac attaacgatg ttgctgctct acgtggtact
    10861 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
    11041 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
    11161 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc cataaactcg cctgcgctgg cggacccgac gttaattact cgcctggccg
    11281 atcgctttgg cgtgcagtgt attgtagtcg gtattgatac ctggtacgac ggcgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacactcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaaatc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg cgatgccgac gttgacggcg cgctggctgc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcag cacgcggtat ccggtgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatcgaaa gcggcaaagt caccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg cgccggactg
    11941 cgacaacgac acgctactgg tactggcgaa tcctatcgga ccaacctgcc acaaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca atggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgtaaatctg ccgacccgga aacctcttac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggccgc
    12181 gacggtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtattgttg caggatcagg atctggattt aacaacggtg attgagaacc tgcgtaaacg
    12301 tcatcagtga gttgcggggt aagcggatgc gatgttgtcg tcgcatccgg caaaaaaaac
    12361 gggcaaggtc tcaccaccct gcccattttc tttaaaaccg aaaagattac ttcgcgttgt
    12421 aattacgcag agcgttacgc cccagcacaa tccccgcgcc aaccatgccg cccagcagca
    12481 ctgccaaaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    12541 tcattacata gcggtaagca tgaatatcaa gatcatcaac ctttaagctt tcgatatcca
    12601 gcaggttttg acgtgtctga tagtagtttg atgagaacac caacggacgg gtcgcctcat
    12661 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    12721 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    12781 cctgaatctg acggatacgc agatctttct gctcctgcgc gactacttcc tgagttttca
    12841 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct ttttccagtt
    12901 cttcgtttac ctggtcgtca acttgctgga tatactgcgc cagctgtttc tgcgcacctt
    12961 cagctgtttc accaacataa gaaaccgcta aaggtaagga ctgattttta acggtaggct
    13021 caatggtcag tttttcaggt tcttcctgat tatccagcgt ttccgctaat gctgagaaag
    13081 cagtgctgta acgtccgata agtgaagtct gaatgtcgga gactttcggt acagccggac
    13141 cgtaaatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    13201 cgatagctgt ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa
    13261 tagccacaat gacagagatg ataattgtca tcttgccgcg ccacaactgc accaggaaat
    13321 caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc
    13381 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    13441 tgaatgctat aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat
    13501 cagggcgaaa atcgcactga taacaagatg ttagtcgctg ccaaaaagat cgcgggtgta
    13561 gactttgtct gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc
    13621 ttgtatagtc agcttagaac agctttgatt gtaaaatgag cacgttacca gaaaaaacag
    13681 ccaggtttag aactgatacc gattatcttt ttttcttgtc taacggtatg atttaacttt
    13741 cagttatgcc agatgaaaat tggctatatt ctagcctgag cgaggattat aaatgaaatt
    13801 tctggttacg ggagctgctg gctttatcgg tttctatgta agtaaacggc ttcttgcagc
    13861 tggtcatcag gttgtaggga ttgacaactt aaatgattat tacgatgtca acctcaaaca
    13921 agcacgactt gatttactca agcacgacaa tttcagtttt tataaaattg acctggccga
    13981 tcgcgagaaa atggccgcac tgtttgcaga cgagcggttc gatcgcgtaa taaacctcgc
    14041 tgcgcaagcc ggtgtacgtt actcccttga aaatcccaat gcatatgcag atgcaaacct
    14101 gattggattc ctgaacatac tggaaggatg ccgccataat aatgttcagc atctacttta
    14161 cgcttcctcc agttctgttt atggcatgaa ccgcaagatg cctttctcta cagatgactc
    14221 tgtagatcat cctgtttcgc tttatgcagc aactaaaaaa gcgaatgaac tcatggccca
    14281 tacgtattct catttgtatg gcttaccgac cacagggctg cgtttcttta cggtttatgg
    14341 tccatgggga cgtccggata tggcattatt taaatttact aaagccatgc tggaaggtaa
    14401 aagcattgat gtttacaact tcggcaaaat gaagcgtgac tttacttata ttgatgatat
    14461 tgccgaagct attattcgct tacaggatgt tattccagaa aaagatccac agtggactgt
    14521 agaaactgga tcgcctgcaa caagttcagc accgtatcgt gtctataaca ttggtaatag
    14581 ttcgcctgtg gagttgatgg actatatcaa tgccttagaa gaggctctgg gtattgaagc
    14641 caacaagaat atgatgcctc tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa
    14701 agcactgtat gacgtaatag gattcaaacc tgaaacgtca gttaaagagg gggtaaagaa
    14761 ctttgtagaa tggtatcgta acttctataa agtttgattt tacaaaacca taagaaaagg
    14821 ccctaatata ttagggcctt ttcttagaat aaaacaaaat aattaatcat tgccaaacaa
    14881 gtcgcgcgta taaactttat ctgctacatc agccagatcg gcagacatac ggttagaaat
    14941 aataacatca gcttcttgtt tgaacgcatc cagatcacgt accacgcgcg aacggaaaaa
    15001 atcgtcctct ttcatcgctg gttcataaac gattactggc acacctttcg ccttgattcg
    15061 cttcataata ccctgaatcg aggaagcacg gaaattgtct gaaccactct tcataatcag
    15121 acgataaacg ccaacaactt tcggtttacg tgcaaggata gaatcggcaa taaagtcttt
    15181 tcgcgtgcgg ttagcatcaa caattgccga aatcagatta ttcggtacag attgatagtt
    15241 tgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa atgagggatt
    15301 gttgtagtga ttaccgatac gcggatcaag gcaaaccccc tcaataattt ggcgggagtt
    15361 aagacccaga ctttcagcgt aactatctag ttcattaaaa taagccacac gcatcgccag
    15421 ataagtattg gcaaaaagtt taatcgcctc agcctcggtt gagtcagtaa acaatattgg
    15481 tatgtcttgc ttaatagcgc cttcctgtaa taacgctgca aaacgttcag cgcgatcgga
    15541 ttgctcacca atcacaatgc gtgatggatg caagttatca taaagagctt tcccttcgcg
    15601 taaaaactcc ggcgaaaaga tcacattttc aataccaaac cgttctttaa tggactctgt
    15661 aaaaccaaca gggatagttg attttataat cattaccgcg ttgggattaa tttctgtcac
    15721 atcgcgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc
    15781 ggtaggcgtg gcaataataa cataatcggc atttttatac gcgtcatact tatctgtcgt
    15841 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg
    15901 tgactgcttc ttgttaagca tgtctacttt ggcctgaacg atatccagtg caaccacttc
    15961 gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    16021 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    16081 cacaacttca tctactgcgg gatttttaac gctaatgtag atgaactgtc aaggcagcga
    16141 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggccc
    16201 taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgcattgatt
    16261 aattgattta aatcagatta atccagccat tcggtatgga acacaccgtc tttatcaatg
    16321 cgtttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    16381 acagcggcac ggtagctatc ataataggca acagcagcgg cgaaggtcgg aaccgggata
    16441 ccgttctgta ctgcgtaagc gacgacatcg cgcagcgcct gctggtagtc atcggcgatt
    16501 tgcttgaagt acggggccag cagcaggtta gcgatctgcg gattttcggc atatgcatcg
    16561 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    16621 tcgccgtagt tcagatccca gttgtactct tcagacgccg cacgcagttg agagaacccc
    16681 tgagcgtaag aaacgatttt gcccagatac agtgcacggc gaactttttc gatgaactca
    16741 gccttgttgc cagctggctg cgcttgtggg ccagagagaa ctttagacgc agcaacgcgc
    16801 tgatctttca gagaagagat gtatcgtgca aacacagact cggtaatcag cgacagcggt
    16861 tcgccgagat ccagtgcgct ctggctggtc catttgcccg tacctttgtt tgccgcttca
    16921 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatgtctttg
    16981 gtgatatcga tcagatagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    17041 aactcttcgt tggaaaggtt caggccgcct ttaagtagag aataggcttc agcaatcagt
    17101 tgcatatcac cgtattcaat accgttgtga accatcttca catagtgacc tgcaccatcg
    17161 gcaccaatat aggtaacgca tggctcaccg tcttcagcca ctgcggcgat tttggtcagg
    17221 atcggtgcaa ccagttcata ggcttctttc tgtccgccag gcataatgga aggacctttc
    17281 agcgcacctt cttcaccgcc ggaaacaccg gtaccaatga agttaaagcc ttctgcagaa
    17341 agctcacgat tacgacgaat ggtgtcctgg aagaaggtat taccaccatc aatgatgatg
    17401 tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc tgcacctgct
    17461 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    17521 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt cttctcacgg
    17581 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    17641 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgttag
    17701 ggggttacta cattaaatat ttaattaata atattaataa cgccaaaaca acttaatcat
    17761 gtaatatttt atttacacaa ttcagtgctg aacggataac ttggtgcata tcataatact
    17821 tatattcagc aaggcgtcct ccgaaaatta ctttactctc catttgcgaa agctctctat
    17881 atttatgata tatatccata ttttttttat cattaatggg ataataaggc tcttgaccct
    17941 cactccactc tattgggtac tcttcagtta taaaggtata ttcagtttca taagagtcat
    18001 caaaatgctt atgctcaatt atcctagtaa aaggaatatc tttttcagta taattaatta
    18061 cggcattgcc ttgataatta gcctcctcaa ttttttttgt tataaacttt aaggatctat
    18121 attctaaagt tcccaacttt ttatcaaaat aagcatctat aggcccagta tatataatcc
    18181 gttccgccat agaattatac ttatcctttt ctgataaata gtcaacatta agcagaagat
    18241 caacaccatt aagcatctta tcaattattt gtgtgtatcc tccgatagga accccctgaa
    18301 atctatcggt gaaataatta ttatcgaaag tgaatcgaac tgggattctt tttataataa
    18361 aaggcggtaa ttcagaacat gaacgtcccc attgtttttc cgtatacccc ttaattagtt
    18421 tttcataaac atcttcccct accagggaaa gagcttgttc ttctaaattt gtgggagtta
    18481 tgttagaata tttacttctt tgtaaatcta ttttatcttg agcttcagaa ggagtttttg
    18541 tcccccataa ctgataaaag gtattcatat tgaaaggtaa gttatataat ttacctttat
    18601 gatatgctaa tggagaatta gtgaatctat taaaagaagc gaatttattt atataattcc
    18661 agatatattg atcgtttgta tgaaatatat gagcaccata cttatgtatg tgaatatcgt
    18721 caactttttc agtatagata ttccctccaa tatgatccct tttctcaatg accaacacac
    18781 gtttatttct tttatttaat tcataagcgc aaaccgcacc aaataaacct gaacctataa
    18841 ttatgaattc atattgcatt gcggttccta tgtcatctaa cttagttaaa attaaagttc
    18901 tttaagtaag caacctttca tatctttatc tgataatata ggattactta aaggccattg
    18961 aatattgagt tctggatcat tccataataa acaacactca tcctcaggat cataatattc
    19021 tgtgcattta tattcaaaat cggccatttc agataataca acaaaaccat gtgcgaaacc
    19081 tggtggtacc caaaattgtt ttttattcat ttcagaaagt aacacaccaa tccattggcc
    19141 ataattaggt gattcttttc taatatcgac ggcaacatca aaaacttcgc cgcgcacaac
    19201 tcttacaagt ttaccttgtg gcttcgtttt ttggaaatgt aatcctctta aaacattttt
    19261 gcttgatcgt gaatagttat cttgtacgaa atcaaattct atatttaata aattttgata
    19321 acgttgtttt tgatatactt ctaaaaaaaa acccctgttg tcaccatata cgaccggttc
    19381 aataatttta actccatcca gaacagtgtc ttgaactttc atatttatta taacctttag
    19441 tgatttctat ttaaaacgtc attaattgta ttagtatact tctctatgat cttattttca
    19501 tcacaatgtg attcagcaaa ttctctcgac ttaatagaca ttgtgttttt catttcgtct
    19561 gtcagtagaa tatacttttc aataatgtca actaagctat caagatcatt aggttgacac
    19621 aaaaaaccat taacttcatt ttgcaccata tccctacagc caacattatc tgttgtcaaa
    19681 atagctaacc cactagaagc accttcaatc aacgctttag gtacaccttc tctataaaat
    19741 gatggtaata ctattgcatc ataatgcttc attatactag caacgtcatc ggaagtccca
    19801 tagtaattaa ctatacccct cgtacaaaaa ccttcaatca attccttcgt gattatcttt
    19861 tcattttttt caaatgcacc taacagtgaa aatgaacaat taggatattt ggattttatt
    19921 atttctgcgg ccttaagata gtgaagtatg cctttggtat atagaagtct agcaacaagt
    19981 atgaatttca cacatgatct ttgctttaaa ttcttatttg agggaagaaa ttttgataaa
    20041 tcaacaccag acccaaatat tcggcatgtt ttctcataat tgacgcagcc agaattaatc
    20101 aataactgac gatcatcctc gttttgaaaa aaaacgaatg ctgctttctt taatgctatc
    20161 ttatataata gcttaacaat ttgacttaat atcgtattac tattaacaaa tgcacttcca
    20221 aggccagaaa tattaggtat cactttcgcg tcaaatttat aagcaagcaa accacaatat
    20281 atattagctt tgggtgtaaa cgttaatata acctgtggat tgaatttttt tataatttta
    20341 taatatgata ttagagatgt acattcagta aaaatattaa tagattttgg tttaagatca
    20401 accttctcaa atttagcgcc gaattttaaa atagaatcct tatattcata atccggtaca
    20461 accactaata catcaaaccc tatattaata agtgccttaa ttgtgcttcg tctaaaatta
    20521 tatatatacc aactactatt agcagttaaa actattttta aggaatgatt atcccttaaa
    20581 tgatcctttt cgtccaagca gtccatcttt tatcccctta aaaatataat ttaatcgttg
    20641 tcctccacgt tcacaaaaag cgtaaataat gattcggaca ggcattgtta tcaatttttt
    20701 taacttatat cttaaaggaa atacaggctc tctcagagct aataaccaat ttctataaac
    20761 ataatacaaa cgaaatggac tgcaaactgt aaatgtttta atccccataa tatttatatc
    20821 tttagatcct atattgtgag aaataaggac tttatctgtc actaggatat tccagccttc
    20881 tttcctagct ctgaaactcc attctatgtc aattaaatcg ataaaccaag atactttatt
    20941 taatccaata ttgataattg cctctagggg tatgagtgca ccagaagata ttatactttc
    21001 aacatcatgt atcgtttcac ttatctggga gcccagcttt atttcacgat catacttttt
    21061 cccattacgt tcatttatta ttgttggacc taatgcagca atattactcc ggagagagca
    21121 agcgtactca tactctttta acattgatga tatataatct tttgatatac atgaatcttg
    21181 atcaaatgta aagaataagg actttcctaa tccgatagct ttaatgaaac catagttctg
    21241 cgcaaacgca atgcctttgt tatctcctaa atgatagtaa tgaatgttgt tattacacaa
    21301 aacatcactt tctaagtgga gctcaggtgt attgtcaaca agtaatattt cgcactgttt
    21361 ttttcccagt gagtcaatca ttttattgat tttactaaca tcaggataat atagtacaat
    21421 tatcaccgct actctattca ttttttattc cacttataag atccatgatg agtccttttt
    21481 acaaaatttt tatcggatac tttaaaggtt atgtatcgat aaaacaaaaa atatcccgac
    21541 aataatacaa tcttccagtc ctgatatata tatgcataca caatagcagc atcaaaaaag
    21601 aaaaagtaat atttaaccct aaataaatca gggtaaataa taaaatcctt taacctttta
    21661 ataccatatt tctccttaca ggcgtgttct aatgttctga ttaaagggaa taataataat
    21721 atgataaaaa caaaaaatac actgcatgtg acagtgggta tcaaaggaat aatgtaacga
    21781 catgacacca aagaaaaata agtgattata tttctcttat cccggattgt attatgcaaa
    21841 taaaaggaga taattaaaaa ccctatacat aatacaaaat agataacagt atagtgatga
    21901 gcaatgatat cactttgata aattaataac gcgaatgata caaaaaaata aacaactcta
    21961 atgaaaatta tacttgataa tttactttta attgtatcac gcagctctcc acctcttatt
    22021 gttggtgctt tttctgtatt tattgttcta tagtcattgt ctaagtagcc tatttcataa
    22081 atggacatgg tcatcataaa tgacagaagc aaagatacaa aactaccatt atacaaaaaa
    22141 caacacagca atagaaaccc tggatatata ataaaccagc taattttttc atgccatgtt
    22201 ttcaatcttg aaatgaataa atatgataat gggaaataca atatcacata aacctcactt
    22261 tatctttgga gacaccaaat ctatttaata gtactctcca tttattttta tctttggctt
    22321 ttgaaataat cactgctgta tctgcaagtt taataagatt aatatcactg aaattatcag
    22381 tgacaatcaa acttacaggg ttattaactt gtaaatgctt tataccagta agattatatt
    22441 ctaataaccc tgttgattta ttgtcgataa attctaattt agatgatact gcctcaacat
    22501 tcaaattttt agcaattaca tttatgacag gatcaacgga agcagacatt aaaatgatct
    22561 tctcatgttt atatcgttct aaaagtgcca taacttcatt cttttcttta aaaatgagaa
    22621 attcatcata aaacaaattc gccaactctt ccaaagtaga cttttcatat ccttctaatg
    22681 tcttcaaaaa atgttttcgt aaggaacata tacgcaattt atgtagcaac tttccaaata
    22741 aggagcgtaa taataagcat ttaattaact tggtatattt tttttgtttc ctatgtacaa
    22801 atacaacata atcaaaagtt gtattcgaca aataaagagt gccacataca tcaaatacaa
    22861 caatatgttc gagcatgtta aactcccttc aaagttagag ttaatcttga gcaaaaacat
    22921 atcacataaa gcattgcaat tgcataaata tagaacatca acatatataa ttggtcaaaa
    22981 aattgaaata acaaggggaa gtacaaaata gagcaaatga caactgatat cgttttctta
    23041 tttttttttg ctacattgta caaagatgaa tgaaagaaac ctatgacaaa aaagaaaatt
    23101 aatgagtaca aaaaatcaga atattttgtt agcgaataaa atattgtaaa aacatttgtc
    23161 ttatactctt tatatgatat aaaattagct aatccacatg tagcatctcc attacaatct
    23221 ataactactg ctttaccttg caaaaccgaa tctaatgcaa aaatgtttgc caagctatag
    23281 ttcatcaatc catattttaa cgcttcgaat gctcctgaag agtcgtcaat tttatttaaa
    23341 aacaacatag ataaatagaa aaaaaatata ataccagaca ctgtgacaag taaatatttt
    23401 aaagatattt ttgaaaaaaa taatcgcaaa aacactattg gtataattag taataaaact
    23461 ttaattttag ctgttgataa taacgcataa aaaagtgaag taaaaaaaac aaaataaaat
    23521 gttcgattat tatatggtaa ccgggcccgc tctccttctt caatcttttt atatttattt
    23581 ttaaaatata aaaagctcca tacatatacc aatgggtata aaagatagta tatataccca
    23641 tatattttta ttaatcccga tttatctccg taattaattt ctgtacgtga atttatatag
    23701 gattcagtga agcccccctt ataataatat aaaaaaacta ttaaagtata gattaaaata
    23761 acaaacgaga taatatttgt aattaatact aacgcttttg tattgtttat attataggaa
    23821 aatacatgtt caaatatagg agcgtaaggt tttgccagta gagcccctac cgaaaaagat
    23881 aaacatgaaa ataaaattat acataatgta tagttagtta tgtctccatt tactcccgac
    23941 aaaaataaca gcccccccca gataaagaat agaatcgccg acggcgattt aatatcttta
    24001 ttaatactca gtaataatcc tgaatacata agcattatta tagctataaa aatggtcatg
    24061 ttcatttaag cttagctcta agttgagaaa ttaattgagc tcctcttcca ctgcattcat
    24121 cagtgttaat tacaaattcc tcaccgactt taaaaacttc tttattttta ttaatccata
    24181 cattcagaag tcgctcagca ataaagccga acacacgtga ttgatattca tcgtatctgc
    24241 ataaatttat ttctttctcc agctcatcta atatggaaaa caaccaatca caataacaat
    24301 caaaaatccc agccttcatt ataaacatat tataaagcga tatttttttt tgttctaaaa
    24361 gttctctgaa tgcaacacta taatcaggat ataatttatc aactattttt tttgccaaca
    24421 ttagatcttc gacattatgg aatttcctgt agtgttcaac aacagatagc ttcaaactct
    24481 ctttcaccgg aataattata tcatattgtt tcattttatt agaaatatta gctctgtcat
    24541 atatcctcac ctctttaaat ttaaaattcg ataaaacatt tccaaaatac ctcctataat
    24601 gaaccaagcc aatataatca tcattaaaaa gtttattttt ccaaagccaa tatataacgg
    24661 tcagctcaca ataattttta tttttacatg atatattatt ccctgtatca tctcccaaca
    24721 cttgatcaag cattttccct gatattttct tacccgcatg aatagggata tagcactcat
    24781 gattaggtaa ttcagtttgt ttatggcaag caatgaataa agccattaat attcctcttt
    24841 atttaaacaa atttattttc tttcttttta agaaaagata catcaatgat gcaactacgg
    24901 tttctgtaat taaaaccgtc atcgcagccc catacgcccc ggcaaaaaag atcaaaggag
    24961 caatgattat cacatgcata acaccacaaa ttagcagtat tttactaaat tgtcttttat
    25021 aaccatgtgt cagcatggtt tgaatgccaa aaaaattact aagagaaata ataaatggca
    25081 aaaaggctaa tattcttaaa atactaattg actcagtata tttatctccg actccatatc
    25141 ttattattat tggagaaagt atgcacagga gtaatgaacc tgaaaacgaa atgaataata
    25201 cgcccttaaa tgattttttc agaagattca tactttttgc ataattttct tcaaacaatg
    25261 aattaattct tggataaaca gcttgataaa taggattcat tagactctgg gctgcatttc
    25321 taattgtatt agcagcgtta aagtagccaa cagagacagg tccacatata aacccaagaa
    25381 caacaacagt tgttgttgta tataagctaa tagcagctgt tgaaatgaaa atatgccatc
    25441 cgctttttaa tgaataaatt ataccatcat aatcaaatac caatttccca atccagttat
    25501 tatttataat aatagcaaat ccaattaaac ctgctataaa attaacgccg ccttgaatca
    25561 aagcggctaa ccagatatct tgtggatttt ttactaacaa aaaagttaat ggtattaata
    25621 ataatcttgc acttaaacta cataatgtaa tccatttcat tttttctaat ccctggaaaa
    25681 accaaacagg gtataatata ttacctaaca ccattggaat aaatgatatt aacaccactt
    25741 gagcataata tagctcctta ataaaaaatg ttattccgaa aaatataaca attgccgaaa
    25801 gtgcaagtat aatcttacta aagaagacat tccaaaatat tgttgttaat ttttccttgt
    25861 cattcttact tttcgaaaca tcttgtgttg ctgaccaatt aaacccataa tcagtcacca
    25921 tcacaagata ttgcattgtt gcttgacaaa accctaatag accaaaagag tatggtccta
    25981 atactctaac aagataagga aatgttaaca atggcagtat aaaataactt ccttgcaata
    26041 tagctaaata gaagatattt ttattaagag aggtaacaag cttcaatgca atctccgagc
    26101 ctatttatat agctgcaact agttaatatt atgatatttt attatttatg atatttagca
    26161 gatactgacc ataagcattt tttttcagtg gttcagctaa tattttcacc tgctcagaat
    26221 caataaaccc tttacggtaa gcaatttctt ccgggcagga aactttcaac ccctgacgtt
    26281 cttcaattgt agcaatgaag ttgcttgcct caatcaggct ttgatgtgtc cccgtgtcca
    26341 gccacgcata accacgcccc atcatggcaa cggataaacg cccctgttcc atataaatac
    26401 ggttaatatc ggtaatttcc agttcaccac gggcagaagg cttaaggttt ttcgccattt
    26461 cgacaacgtc gttatcatag aaataaagcc cggttaccgc ataattactt tttggttgta
    26521 gcggtttttc ttccaggctt attgccgtac cgtttttatc aaactcaacg acaccgtagc
    26581 gttcaggatc attaacgtga taggcaaata ccgttgcacc actttctttg tcaacagcgg
    26641 tatccattaa cttcggcaga tcgtggccgt agaagatatt atcaccaaga accaaagcac
    26701 aatcatcacc accgataaac tcttcaccga taataaacgc ctgcgcaagc ccatctggag
    26761 tcggttgcac tttgtactga agatttagcc cccactggct accgtcaccc agcagttgtt
    26821 gaaaacgagg agtatcctgt ggcgtactaa taatcagaat atcgcgaata cccgccaaca
    26881 tcagtgtaga gagcgggtaa tagatcatcg gcttatcata aataggtaat agctgtttac
    26941 tgacagccat agtcacagga taaagacgtg taccagaacc acccgctaaa ataatacctt
    27001 tacgcgtttt catttcatca ttccttttaa ttcatcttgc tccatcatca cgaacaagat
    27061 gcaaaaacta ttaaattgct gtagtcgtaa ataattcgtt gagcattcgt ttcacgccaa
    27121 cctgccagtc aggcaaaaca agcgcaaagt tctgctgaaa tttttctgta ttaaggcgag
    27181 agttatgtgg acgacgagct ggtgtaggat aggctgttgc tggtactgcg ttgagcttgt
    27241 tgagtgcaag gggaatacct gctttgcgcg cctcttcaaa aaccagcgca gcataatcgt
    27301 gccaggttgt ggtaccactg gctaccagat ggtacatgcc tgcgacttct ggtttattca
    27361 gtgccacacg aatagcatgt gccgtacaat ctgccagcag ctcagcacct gttggcgcac
    27421 caaattgatc attaatcaca gccagttctt cgcgctcttt tgccagacgc aacatcgttt
    27481 tggcgaagtt atttccttta cctgcgtata cccagctggt acggaaaata agatgcttcg
    27541 cgcaatgttc ctgtaacgct ttttctccgg ctaacttggt ttcaccgtaa acatttagcg
    27601 gtgcggttgc atccgtctcc agccatggta tttcaccggt tcccggaaat acgtagtcag
    27661 ttgagtaatg aattacccag gccccaactt cattagccgc tttggcaatt gattcaacgc
    27721 tggtcgcatt gagtaattgt gcaaattcgg gttctgactc agccttatct actgcggtat
    27781 gagccgcagc attaacaatt acatcagggc gaattttttt gacggtttca gccacacctt
    27841 caggattact aaaatcacca caataatcag tggagtgaac atcaagagca atcaaattac
    27901 ccaaaggtgc gagagcacgc tgtagttccc aacctacctg ccctgttttg ccgaaaagga
    27961 gaatattcat tactggcggc cctcatagtt ctgttcaatc cacgattgat aagcaccact
    28021 tttcacattc tcgacccatt ttgcattagc caggtaccat tccactgttt tacgaatccc
    28081 gctctcaaac gtttcctgcg gtttccagcc caattcgcgg ctaatcttct ctgcatcaat
    28141 cgcataacgg cgatcgtgtc ccgggcgatc ggcaacataa gtaatttgct cacggtaaga
    28201 tttctctttc ggtacaatct catccaacaa atcacaaata gtgagcacta catcgatgtt
    28261 tttcttttcg ttgtgtccac caatgttata agtttcaccc gctttacctt cggttacgac
    28321 gatatataac gcacgcgcat gatcttcaac atacagccag tcacgaattt gatccccttt
    28381 gccataaata ggtaatgcct taccttccag agcattaaga attactagtg gaatcaattt
    28441 ttccggaaag tgataaggac cgtagttatt cgaacagtta gtcacaatgg tcggtaaacc
    28501 ataggtacgt ttccacgcac ggactaaatg atcgctggat gcttttgatg cggaataagg
    28561 actactaggc gcgtaagctg tcgtctcagt aaagaggggt aattgttctt tattatttac
    28621 ttcgtcagga tgaggcaaat caccatagac ttcgtcagta gaaatatgat gaaaacggaa
    28681 gctatttttc ttgtcgccat caagagcaga ccagtaattg cgagccgctt ccaaaaggac
    28741 ataagtacca acaatattgg tttcaataaa tgccgcaggg cctgtaattg aacgatccac
    28801 atggctttca gcagccaggt gcatcactgc atccggctga tgctgagcaa aaatccgcgc
    28861 cattgcagca gcatcgcaaa tatcagcatg ttcaaaaaca tagcgttcag agtcagaaac
    28921 atcagcaagt gattccaggt ttccggcgta cgttaattta tcgacattaa caacactatc
    28981 ctgcgtatta tttataatgt gacgaactac agcagaacca ataaatcctg cgccaccagt
    29041 aacaagtatc ttcactttat tattccatat tacttcagag catgctgtga aataagcggc
    29101 tctcagtttg attaatagag gtattaatgc acgctaccgc ccctggcttt acagctacca
    29161 gagcactgca tgcatgtcta cgatgtgacg agcgttaccc actcgcgcta aacccgaaaa
    29221 attcaaacgc taattgtctt accaatccgc tctggaaaca aggaaaatcc tggaaaactt
    29281 tgactaaaat cctattgcta actcgttgtt atcctgattg tttatataaa acaacggcag
    29341 gaatattcgc aacaaattac tttcgccacg aatcttcact gccgttataa ttttcttatc
    29401 aaccgttaca tccggtcaga ttttcattat tcgcttaaca acttctcaat ccctttacgg
    29461 aacttcgccc cttctttcag gttgcgtagt ccatacttca cgaacgcttg catatagccc
    29521 atttttttac cgcagtcgta gctgtcacct gtcatcagca tggcatcaac ggactgtttt
    29581 ttcgccagtt cagcaatggc atcagtcagc tgaatacgtc cccatgcacc aggctgagta
    29641 cgttccagtt ccggccaaat atcggcagaa agcacatagc gaccaacggc catgatgtct
    29701 gagtccagcg tctgcggctg atccggtttt tcgataaatt cgacgatgcg gctgacttta
    29761 ccttcgcgat ccagtggttc tttggtctgg atgaccgagt attcagagag gtcacccggc
    29821 atacgttttg ccagcacctg actgcggccc gtttcattga aacgtgcaat catggcagca
    29881 aggttgtaac gcagcgggtc ggcgctggcg tcgtcgatca caacgtctgg cagcaccacg
    29941 acaaatggat tgtcaccaat ggcaggtcgt gcacataaaa tggagtggcc caaacctaaa
    30001 ggttcgccct gacgcacgtt cataatggtc acgcccggcg gacagatgga ctgcacttcc
    30061 gcaagcagtt gacgctttac gcgctgttca aggagagatt ctaattcata agaggtgtcg
    30121 aagtggtttt cgaccgcgtt cttggacgcg tgagttacca ggaggatttc tttgatccct
    30181 gcagccacaa tctcgtcaac aatgtactga atcattggct tgtcgacgat cggtagcatc
    30241 tctttgggaa tcgccttagt ggcaggcaac atatgcatcc caagacccgc tacaggaata
    30301 actgctttta aattcgtcat tatttcatcc acctgtaaaa tggttgctga attatagctt
    30361 gttcgatttt tttcgccagc atcaattacc ctgaattgat tactgaatta cttgtgatgt
    30421 tacgccgctt cgttgtggat tgcagtagca ttgttcctaa gtatggctcc atttttccag
    30481 gaatggtcgc aaatctactc cccccgttcc ggcaatctaa agttaatctt ttccacatta
    30541 acaatatggt gattaatcct gtcgatatcg acggagcttt gccctctttc attcaccgca
    30601 tgaacatttg caagagatag tagtgtttct tttttcgcca taaagacgcc gcgaacgtct
    30661 ttgcgcatgt cgaagttcat gctcaatgct ggtccaacag aggattcctg catcacatta
    30721 atattacgca taaaaaggtg ttgtggtttg ttgtgtaact ccagcgacgc acgcttcatc
    30781 tcaatgttag tcagcgccac acaggagaca gcattcccgg cggagatttg gatgccgcgc
    30841 aatttataag caagatgggt gttatctagt tgaatattat tcactcggaa attttgcggt
    30901 atcgagagat atttgccttt aattacccca tagccgatta acatcccggc gctattaatc
    30961 atttcaatat tatcaatcac gaaattgtca caaccgtaaa tagcaactgt cgcgttatca
    31021 atacctgctt tcttactgaa atccggcgtg atattgcggg ctttgatatt acgaataaca
    31081 aaatgtttac cattttcaac atggatcagt tgccgacaat ccgatcccgt gatattcgcc
    31141 acgacaaagt ttttcactgc ctggtcttcc ggataattat tatcgtaggt acttcccgca
    31201 agacctatgc cgatgcccca gttgattttg ccgttggtac agttgatgcg ctcaataacg
    31261 tggtcagata tcaaaatatc actgtcgtta atcgccacgt tccattcaat ggcatcgccc
    31321 tgtaagtcgc tgaacttaca attggtgatg ttggcaccga taatctggtt atgaaatccc
    31381 tggcgtaaga tggcgtaatt agcgtggcta acggtcaggt tatcgatggt caggttgcgc
    31441 atgacctgtt tgtttttgcc gccgatataa atctgcgtta ccgggccaaa gccgctcata
    31501 gtcagccctt tgatggtgca gtcggaacca cgcacatcca gggtgatgtt atgcatactg
    31561 ccgccctcct cccctgtcac ctggctgccg tcctgtaaaa caaatcgccc tctgccgttg
    31621 ccgcgcaggc ttccaaggat gtgtaacgtt ttacctgcag gaatgaagat gccggtgttg
    31681 atattgtcgc aaaccaatcc gccaggcacg acgaccgttt gcccttcgct gaaggcttgt
    31741 ttaaatgagg cgatccagtc gtgagggttg tagtcgttaa tgttaacgct ttgtcgggcg
    31801 ggaagtgcgc gagcgaaagg ggtatggagg aaggcaagcg ccgagcttgc cgtcaggaag
    31861 gtgcgtcggg agagtttttt aaatggcatg cgttctcctc tataaagcct gcaacaagct
    31921 ggcgagttct cgattaatca cctgctggtt aaaatcgtgt tcgacttttt cgcgcgcacg
    31981 tttgacaacc gtagtaagtt cgtcggtgtc cagttggcta aacgccgcca gtcgttgcgc
    32041 cagtgcgcga gcatcgttct caggcaccag ccagccggat ttatcggcct ccaccagttc
    32101 cggtattccg ctatgcagag tagaaaccac cggaatgccg accgccatcg cttccatcag
    32161 cgccaccgga atgccttcca tatcgccgtc agcacccgtt accgatggca acagaaagac
    32221 atccgcatcg tcgagcatcg ccttcacttc gtggcttggt ttaaagcccg gcatctctac
    32281 cacatcctcc agttgatatt gttcgatgag cgtgcgcagg cgtcgttccc acgggccaat
    32341 gccgaggatg cgataacgaa atgccacgcc ctgctctttc aactgccggc aggcttcgat
    32401 cgccacatgc aggccttttt tctcggttaa gcgtgcgaca gatatgattt ccagcggcgt
    32461 tgcaggcgct tttaccggac gcgggctaaa gcgcgtcatg tctacaccca tgcgcgatac
    32521 ggcaattttt tccctcgggc agcccatttt ttgcagcctt ccggcccaca gttcgcttat
    32581 cggtagcatc aaatcgccac gacgaaacag ttgttgatat tcgggagtgt aatggttgag
    32641 cacttcccga ctggagatat caataccgtg gaagatagtg gcaattttgc cgcgaatgac
    32701 atccagttcg cgtagttttg ctgcggttac ccccgcaggg ccaaaatgag cgataaaaac
    32761 atcagcatga aacggtgttg cgacctggcc gcaaatggca gacaaaatca ggttccgcga
    32821 ctcagcacca tagcgtttga ggttgagcgc ctgccaggta tttttacgat gaatgccgcg
    32881 taaggtctgg ctggcgcggt agcgcagttt cgccactttg ccttgtggtt cgtcctgtaa
    32941 ccagcgagtt ctggcggcaa ggttatattt cgtccatgcc gcgtgggtgt tttgcgtatc
    33001 gcctttttgc agcgcgacaa tctccacctc aaagcccata tcaataaacg cagtgatttg
    33061 gttgagaacg aaggtttccg acgacagcgg aaatttcaat aagaagaagc cgaccttcat
    33121 ttcgcctccc cgatgcgctc aagcacagac tgcaccatcc gcattcccgt ctgacgctca
    33181 cgactgacgg cttcgttaag tcgcgcgttc agcgccggaa gttggcctaa ggtatccgca
    33241 accatcgctt gcaggctgcc gtccagtaaa tggcgaatat caatcgccat ctctggcagc
    33301 cccagttgtt gcataatccc ggcggatttg tgttcgtagt tgatggcaat tgccggagtg
    33361 gagaaattca tcgagataat ggcagagtgc aggcgcgtac ctacggtgag ttcacaggca
    33421 ccgaggattt tgcccatttc cagatcgttg agctcatcca tcaccacgtg ataacgggca
    33481 ggatcgctga tgtgctgacg caggttgagc gccaccatgc ggtcgtcttt gttatagctg
    33541 tcgatacccg tacaggtgga gagcgcaatc acctgatagc cttcgtcgag aatgcgattg
    33601 accaccccgg caaaggcttt ttcatacgct tcttgagtgg tgccgagacg tttgtcgaac
    33661 ggtgccagtt cgcgcagggt aattgccacc gctttctgtt gtgcggcaac atccagccag
    33721 tgttgaacag catagctggc ggtgaagtct tcaatgtggt gatcgaccag ccacgcggta
    33781 tcgacgccat tttcaacttt tgcggtagtg atattgctgc gtttcattag atccaggctg
    33841 accgattcgc gcaggatcag cgcgtcgcag tgaccgaata cgtagttcgc cagttggtta
    33901 aattgctcat cctggaacgg gccgacgctg tgaccaatca taaacagcgg ctttttcgcc
    33961 ataaacgtgc aaagcgcatg ttcaaactgc ggcacgccgt agagatcgac aaaaaacgat
    34021 ccgccgacct ggataatggc atcgtaacct gacagcaggc gcacaaagtc agtgaatccc
    34081 tgggcaatgg cgatattacg cagcttgccg gtgtcggtga cgcgtgagag caatacctgg
    34141 tgctggtagc ggcgacggag gacttttttt acgcgcccga caacgcccgc cgcgctgttg
    34201 tgttgtttca tttgcaggaa cagcggatcg cccattaccg gacggttgag cagccaggaa
    34261 gaactgaccg gatagcggct cattacgtcc acttcggcgt gttgattgag gatgttgatg
    34321 gcatcaagta agccgcgcaa gatggcgctg tcgccgcgat tgccgcaagt gtggttgccc
    34381 agaatcagta atttcatttt ttcctcataa atttgatgcc aagtgaggct gtgttttttg
    34441 tgttcgttgc cgctttttgc ctggcgcgtc ttatcaggcc tacaagtccc gagcacagag
    34501 ccgtaggccg gataaggcgt tcacgccgca tccggcaact attgccggaa ccgaaaacaa
    34561 ccattcaccc ggcccgcaaa agcattttca ttttttcgct gcgacaaaac tgacgcttca
    34621 cttccaccac cagcggatgg cgcgacaaca cgatcatcac cacaaacgcc agcacgcctg
    34681 cgataatttg caccgccagc agcatcccca gcgccagttg ccctttcagc acaatgccca
    34741 gcgcataact gaccaccagc gtcggcaacg agagataaaa cggcagccac aaactcagga
    34801 tgtactggcg ataactggaa ccaagaaccg gtttaatcat cacgaagtaa ctcagaatgg
    34861 tgttgataat ttgcaccagc aggaagccaa gcgtgacgcc gatcgcgccc gccatctgcc
    34921 caccaataac aatcgccgga ataaacagaa atgttttgaa tacgttgaat ttaaagctga
    34981 tatcgacccg cgctttcgcc atcagcagcg aaccaatcgg gttacccacg gagcgcagca
    35041 gacccacaat acacagcaat tgcagcaccg gaataatgct gttccacttt tcgccgaata
    35101 ccagcggtac gaagttattc gacaccacca tcagcccgag cagcgccgga aagttgataa
    35161 tccccaccac cgacagcagc ttgtagaagt tcacgcgcag cttttcggtg tcgtcctgaa
    35221 ttttggcaaa cgccggaaac aacacacggg tgatgattgg gttcagcttc atcggcggca
    35281 caacggccac gttgtacgcc aggttgtatc cccctgccac gcccgcgcca agaatacgcg
    35341 ccagcaccag cgttgaaagg ttggtgttga gatagttgat gatgctgtcc gccgtcagcc
    35401 aggcaccaaa acgtaagttc ggcgcaactg acgccagcga gaaatgcaga ccggggcgat
    35461 aaatttttcg accaaagtag ccaaacagca gcgttcttac cgcactattg accagataac
    35521 cgaggatcgc ggtcatcgcc agcggccaga aatgggcgct aaccaccgta aaagtgaagc
    35581 ccgccagcac cgcgctggtt tcgatcatgc cgattttgtt gaactccagt tccttttgca
    35641 tcaacgcgcg gaactgttgc ccgtggggga tcaccacaaa cgccagcgac aatgttttaa
    35701 tcaacggtgc caggtccggg ttattcagca cgtcaccaat ggcatcactc aacaaaaaca
    35761 ccgccacaca caccacgatc cccagcccga cgttcagcca gtacagcgtg gtgagttcca
    35821 ggtggctgat ttcttttcgc tgaataatcg agttagcgat accgaagtca gaaagcgtat
    35881 ctgccagcgc gatgatcacc agcgacacgg taagcaaacc gaactggtgg ttgtcgataa
    35941 tccgcgccag cacggtcatc tgcaccagcc cgaggccgat gatgatcacc gtagcaatcg
    36001 ccgaccactt tgcgccgctg atggtctttt cacgtaagct catatcaata tgccgctttg
    36061 ttaacgaagc ccttgaatac cgtcaggaaa acgattttga tatcgaacca gacgctccat
    36121 tcgcggatgt actcaaggtc gaactcgacg cgtttttcca ttttctccag cgtgtcggtt
    36181 tcgccacgcc agccattaat ctgcgcccag ccggtaatgc ccggtttcac cttatggcgc
    36241 agcatgtagc cttcaatgag ctggcgatac tgttcgttat gcgccaccgc gtgtggacgt
    36301 ggaccgacaa tcgacatccc cccggtcagc acattgataa actgcggcaa ttcatccagc
    36361 gaggtgcggc gcagaaagtt ccccactttg gtgacgcgcg gatcgttctg cgtcgcctgg
    36421 gtcaccactt tgtcgttctc catcactttc atggaacgaa acttccacac tttgatcggc
    36481 ttgccatcca tgccatagcg agtctggcgg aaaataaccg gccctggtga actgagtttc
    36541 accgccagcg caatacagca cagcaccggg gagatcagca acagaataag cgtcgccagc
    36601 acaatgtctt ccgcacgttt gagcaggcgg ttaaccccgg aaagcggcgt gtcgtaaagc
    36661 ggcaccaccg gaacgccgtt catctcttcg agacgtgaat ggagaatgtt gaaggtaaag
    36721 acatcgggga tcagcagcac cgaacaggtg gtatccgcca gttgatggac cagttttttc
    36781 actcgcgcgc cgtcgcacat ctgcatcgcg atatagacgt tatgaatttt gcccgctttc
    36841 gcatcctcga ccagctgttg cagattgccc gcccagtcgt tagaaacgcc gcccggtttc
    36901 gggtcgtggt aaacccccac cacttcaaac cctaaccacg gctgattacg gaagctctcc
    36961 atcagcattt gtccggcggc taaatctccc gctacggcga ccatacgctt gttatagcca
    37021 tgattacgca gccagcccgc cccaatgcga atacatgaac ggcaaaccac cagtccgata
    37081 ctggtcagcc cataccacgc cagccagatt ttcagttgcg tgtcgaaatc attgttgaac
    37141 gccaccagtc cggcgctgaa aatcacgctt aaggtccagt tttgtagcaa cagggcaaat
    37201 tctgtcgctg cccgaacacc gcgccatgag cgataaaaat cggtgatgcc gcccagcatc
    37261 tggaacacca ccagcgtaat caacgccacc aacaggtgca tgtagaggaa tgacagtccg
    37321 ctcacttcgc aaaccagcca tagtccggca aacatgatgg tgatatctga aaagcgttgc
    37381 accatagaga ttaacgatgc attggttttc gctcgctcgc gcttttttag atttgtcatc
    37441 gttgttcctg ttattagccc cttacccgca gtggggtaag ggaagatccg acattactca
    37501 ttcagcaacg tcagcagagt tcgcgttcgc gcttccatca gcggcacatc accgcgcgat
    37561 tccacattca accgcaccac cggttcggtg ttggaggtgc gcaggttaaa gcgccagtcg
    37621 gcaaaggtca tgctgatgcc atcggtacga tccaccgaca gcgcctcacg gctaaaatgc
    37681 tgttcgacgc gattaatcgc ctcaacgggt tgcgccagtt tgctgttgat ctcaccgctt
    37741 gccggaaacg ccgccatccg gtcgcgcacc agttcgccca gcgtttttcc tttcaggcac
    37801 accagttcgg ctaccagcag ccacgggatc atgccgctgt cgcagtaagc gaaatcacgg
    37861 aagtaatggt gagcgctcat ttcgccaccg tagatggcgt cttccttgcg catccgttct
    37921 ttaataaagg cgtgtccggt tttcgacatt accggggtgc cgcctgcggc agtcaccaca
    37981 tcaatagtgt tccaggagag acgcggatca tggatgatct tcgcgccggg atttttttcg
    38041 aggaacgctt ctgccagcag gccgacaatg tagtagccct cgataaactg ccctttttcg
    38101 tcaaacagaa aacagcggtc aaaatcgcca tcaaaggcaa tgcccatatc cgcgccgtgt
    38161 ttgatgaccg cattgcgggt gtcgtcgcga cattccggca gcagcgggtt aggaataccg
    38221 ttggggaaat tgccgtccgg tgtgttgtgt actttgatta attccacggg cgcgccgagg
    38281 gctttaaagc gggcttcaat ggcgtctacc accggacccg ctgcgccatt cccggagtta
    38341 atcaccagct tgagcggcgt gaggtttttg acattgatat aaccgaacag gtgatcaacg
    38401 taagcgtcac gcaggttgat ttgctgatag cggccgcgtt tggtttcatc gacgggagga
    38461 aagtcgttgg cttcagccag acgctggacg tcgcgcagtc cggtatcacc gctgatcggg
    38521 cgagcccctt cgcgcaccag tttcatgccg ttataatcca tcgggttatg gctggcggta
    38581 acttcaatgc cgccatccac gccgagatgg aacgtggcga aatagatctc ttcggtgccg
    38641 gacatgccaa tatccagcac atcgacgccc gcatcctgta aacctctcgc cagcgccagt
    38701 tttaaggttt cgctggtgag gcggacatca ccgcctaaca caatggtttt cggtttgaga
    38761 aattcgccat aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctagt
    38821 tttccgcgaa tatcataggc tttaaagcag gttaattttt tcatatcgtt accctttttc
    38881 aggcaatagt tggccctgac cgaagcggcc aattttattt ttgtcgttat ttacaccgcg
    38941 gtttcgcatt cattgcctga tgcgacgctg ccgcgtctta tcaggactac aaacccgaac
    39001 cgtaggtcgg ataaggcgtt cacgccgcat ccgacaaaca gctcctgatg cgacgtttac
    39061 acccgtccgt agcgatccgc gaagcgcacc acatcatcct cttcgagata agagccggag
    39121 cgcacttcaa ttaaatcgag cggaattttc cccgggtttt ccaggcagtg cgtcgccccc
    39181 agcggaatat aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact
    39241 tttgccgttc ccgcgaccac tacccagtgt tccgcgcggt gatggtgcat ctgtaccgac
    39301 aagccctcgc ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata
    39361 gagtcatatt tgccccacgg acgatacact tcgcgatgca cccgatgctc atggcgacca
    39421 tcggctttga tctgctcgac cacttttttc acatcctgca ccgcgttacg gtcggcaatc
    39481 agcaccgcat ctttggtctg caccactacc agatctttca ccccgacggt ggtgaccagg
    39541 ccagattcgg cgtacacata gctgttttca gttttgtgat taatcacatc accgtggcaa
    39601 acgttgccct cggcggtgtg ggcgctgatc tcccataatg aagaccagga accgacatcg
    39661 ctccagcccg catccatcgg caccacaacg gcatctgccg tacgttccat gaccgcgtaa
    39721 tccaccgact cttccgggca ggcgagaaac gcttcttcat ccacgcgaat aaaatcgaga
    39781 tccggatcga cggcgctcat cgcttgttca caggcgtcga gaatatccgg gcgatatttt
    39841 ttcagttctt cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat
    39901 tcgccgcttg ccacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc
    39961 acttcaaagg ccaccgtatc ctgctcaccc gcagatactt caccgcgacg aatatagcca
    40021 tatccggttt caggtagatc cggcacaatg ccgaaggtca ccagcttgcc cgcttcggca
    40081 tacggcatgg cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc
    40141 gccgccaata ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc
    40201 gccagtgcaa tggcaggtgc agtgttacgc cctgccggtt cgagaataat gttctcggtg
    40261 agtttgttca gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc
    40321 accaccgggc tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg
    40381 agatcgcctt tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataag
    40441 cggctaccgg agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc
    40501 cccgaatatc atttataaat tgacgtaaca cgttctcttt atcgagtgtg cgttcggcat
    40561 attcacgtgc caccgtgttg tgtttgggca gcaggagcgc ctgacggatc cccgccacca
    40621 gcgcctcgac cgattccggt tctacgcaaa ccgcaatgcc cggaaaggtt tcgcaaagct
    40681 gccccagttc tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat
    40741 tggtcagttt cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc
    40801 aatcgcccat cttcagcagt gcgggtaaag cgtcatacga ttgcagcggg aaaaattgca
    40861 tgttgcgcag tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc
    40921 cgacaatggc aaaaatcagc ggttcatcgc gcagacgatc ggcagcttca ataacgtttt
    40981 ccagcccctg cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca
    41041 ggtcaagctg gttacgaagg gcgtcaacat cggcatctgc aacatgctga aaacgggcaa
    41101 tttccgacca gttggggaag aagatgacgt tttccgccgc cacgcctttt tcgatggctt
    41161 tattcatcat cgaacgcgaa atcgtggaga cgttatcgac gttatgcagt ccgctacgtt
    41221 cgaacgccgt tgccagctgt gccactttgc cgcctttgcc ttttccggca aggcccagcc
    41281 ccagcatggc gtccacttcg taatcctgaa tatgcagcac ggtacgcgca ccagagagct
    41341 tcgccagcag gcgcattccc ggcgtgcaaa acagcgttgg cactacgccg ataatgcgat
    41401 ccggcttcca gcgacgttgc gccatcagtg gaaagaaact gctgacggca aaactgccga
    41461 gatgcaacaa gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca
    41521 ccgtggcggc cccctcttct cgtttgtagc gccaggcgga atagttctca cccacctgcc
    41581 attgcgggta gtaaggcggt gcggtaatga cccgcacctc atgaccttgt gccgccagcc
    41641 attccaccat ctcgccggtg tatttgccga tgccggttaa ctccggcgag tagttaatgc
    41701 cgtagaccag tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc
    41761 gcggctgtta gcgtgaacat cgttactggc gagcagcgcg tccggcgtca gccagcggta
    41821 atcgtcatgc tgctcatccg gcattaacag ctcttcttcc gctactctga agcgaaaacc
    41881 gagcaccaca tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaagtgctg
    41941 ccagacaccg taaaactggc ctgctgttat cggcagacgc agccccagtt ccgccatcgt
    42001 cagccgctca aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa
    42061 ccagtaaccc tgcgccgggc ggttggttct tttgccaagc agaaactcgc cgcgactgtt
    42121 ctcgacaata aagtcgagag agacaagcgg tgtggagcgc actacagtgg caaagtcttc
    42181 ctgacgtaaa aacatcattc cccccgaaag cggtcttgat tctcaaggaa ccactggtaa
    42241 gtgctggcaa gccccgcttc cagtgagatt tcgtgatacc agccaagctg atgcaggcgc
    42301 gtcacatcca gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga
    42361 cctttgtaac ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg
    42421 cccgtaccaa cgttaatgtg cgacagcatc ggctgggtgt tctccagcca gacttcatgc
    42481 gccagctcca tgacatgaat gctcgccgcc gccatgtcat cgacgtgcag gaattcacgc
    42541 atcggtgtac cgctgcccca taccaccacg tccggcgcat tctgtgccgt cgcctcgtgg
    42601 aagcgacgca gcaatgctgg gatcacatgc gagttactcg ggtggaagtt gtcatgcggg
    42661 ccatacaggt tggtcggcat gactgagcgg taatcgcgtc cgtactggcg gttgtatgat
    42721 tcgcacagtt tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc
    42781 gtgccctgca ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatacag
    42841 gacgatccaa gaaacagcag tttgttcacg tcgttctgat gcgcggcgtg aatgatgttg
    42901 ctctcaatca tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg
    42961 ccgcccactt tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca
    43021 tgcaccgcgc ggctgtccag caggttcagc tcgtcgcggg tgcgtaatac cagttccaca
    43081 tcaccgcgct gttcgagctg ccgcctgatg gcagaaccga ccattccgcg atggccagcg
    43141 ataaaaatgc gttgtctact catgcttatg actccagcgc gatcgccacg tcgtagccgt
    43201 gagatttcag cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca
    43261 ccatctctct gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt
    43321 cgccgagcag cgtttcaact tcagccggac ggaagtaacg cggatcaacg gcgataatca
    43381 catcacccgg tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct
    43441 cttcaacgcc cgtgccttca aagcgcagtt tgatgcccag ctgtgctgcc gccatttcca
    43501 cgaactgacg cacggagtac tgaacgccgg tcgcgataac gaaatcttcc ggctgttcct
    43561 gttgcagcat catccactgc atttttacgt agtctttggc atggccccag tcacgcaggg
    43621 aatccatatt gccgaggtac aggcacgact ccagcccctg ggcgatattg gcgattgcgc
    43681 gggtgatttt gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa
    43741 taccgttaca ggcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcgt
    43801 acagtttggc gacagcatac ggagagcgtg ggtagaacgg cgtggtttct ttctgcggaa
    43861 tttcctgcac cagaccatac agttcagagg tggaagcctg atagaaacgg gttttctttt
    43921 ccagaccgag gaagcggatc gcctcgagca ggcgcagcgt acccatcgca tcgacgtctg
    43981 cggtatattc cggtgactca aaagaaaccg caacgtggct cattgcgccc aggttgtaaa
    44041 cttcatccgg ctgcacttca cgcaaaatgc gtgtcaggtt ggaggtatca ctcaggtcgc
    44101 cataatgcag atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc
    44161 gctcggtgtt gaatgacgat gcacgacgct taataccatg cacctcgtaa cctttttcca
    44221 gcagaaactc tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt
    44281 ttgacatgtc ttattcctct gtattttttg aatttattca gtttcaacgc gttcgcgtat
    44341 caccactgcg ggattccccc ggcaaaccac atttgccgga agcgttttaa aaacactgct
    44401 tcgtgcaccc acgacggtgc cgtcgccaat cgtgacgcca ggggcaacaa aaacatcggt
    44461 tgccagccag catttctcgc caatcacaat aggagtagca ttaatggtga aatgttgact
    44521 tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg aatgtgcgcc
    44581 aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccgacccacg cgtaatcacc
    44641 taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta cgttttttcc
    44701 tatttttgcg ccgaataaac gtaataaaaa tgcccgccag cgatacaata cttgtggcga
    44761 ccaggcaaat attgttgcct gtactgccca ccataattgc actttaatag cgttgccgcc
    44821 ccggaaccct ttcggcaccg agaaaccgct taaatcttgc attggttctc cttatattca
    44881 gactttgtta tataaggctt tcgtctttga ggtagtacgt tggcgtaaat gccaggataa
    44941 ttcagcccaa aagccaggta catgtaatat ttgtcgttgg acttttttcg cgtcagcaca
    45001 caattccata ttattggtgg tagatacccc acccatggaa aattcagaca ccaggccatt
    45061 gagtttttta aatgcataac cggctttata cattttggct gccagcgcgt agtcggaaga
    45121 aactttatat tccaggtcat aacgccattt tttcaagccg gatacaggga aaaatatcgc
    45181 ctgatgactg gcgggcaggc tgtgataaat ataccagccc ggtttggcgc tacgtttaat
    45241 tttatgcccg tcgccaaaat ccagcagcgc atcgccggtg atcatcatgt tatctttttg
    45301 catttttaac ttacggacaa aatttgcggc atcctgatga aaaatatcgc ccgaattgag
    45361 aaacaacgcg aacttgcctt gtgccatcgt aatgcctttg ttcatggcgt cgtagatacc
    45421 gttatctggc tcgctgacaa agcgtaggtt atagatacca ttgagatttt ccagatactc
    45481 acgggtgccg tcattggaac cgccatcgac aacaatccat tcgaagctga tatcttccgc
    45541 ctgcgccaga tgcgccagcg aggcatgtgt tttgactatc ccttcgaggt tacgaaacgc
    45601 gacagtgatt atgctaagca acatgttctt attcttacct cgtaatattt aacgcttttc
    45661 gcaaaataaa cggacagacg attaaaaatg catattccgg gctaaatatc gaaccggtaa
    45721 aaaacagcga taccggagta aaaagatata attgcacgcg aaaattgcgg ttatcaccaa
    45781 aagcgttgat catcattttt atcactttcc ccatgtacca cagcgataaa aacactgcga
    45841 accaggaaaa ataaataatc agcagataca aaccattgtc tatggttttt ccgacatccg
    45901 caccgttaaa tattccgaat gatgcgacgt attcataaag tgagccaaat ctgactacac
    45961 cgtcaatatg ggtcaaagaa taaccgacca tcaccagcgg accgacaata cgataatacg
    46021 atgacgatcc ttccgtacct aaatcgccca gacgggtgga aatataagga aacgcgatta
    46081 ccacaccaac caggaataca gccagagaaa tcaatgccaa cggtaacttt ttcttaatcg
    46141 cctctttatt cagatattga aatgcccact ccagcaaata aaacaggata aaggtcataa
    46201 cccctgaaaa cgatccggat aatattatcc ctgcgagaat catagcatcg gttttaggcg
    46261 ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga gcgaaaaatg
    46321 ccggttcgaa ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa ttcagtacat
    46381 agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg ccaccggtga
    46441 ggataatttg cgccatctcc accaccgcca gcgccaccac cagcccgacc accagataaa
    46501 agaaacgtaa tatcttgcga tggttgtgtg gcgaaatcgt tttaaagcga atactccaca
    46561 ccatgccaat aatgatcaca atatagacaa acagcatggt tgatgtgacg tatttactgg
    46621 catccaacga ctgaccaaac agatagttga acgccgtgag tcccgcacca atccctaagg
    46681 caatcatcaa ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag acaggtaaaa
    46741 aagtgacgat agtgatgggg aaactttcac caagctgggc gattttgacg ttaaccagca
    46801 ggtagatcag cggcagcagc aggtagctac agattctgat aggagttgac atactcctcc
    46861 agcatctgtt gtccactgta ggcggcgcgg ctgcgttggc tgaactcagc cagcgtggta
    46921 ccaaatatcg cctgcgcgat ttccggtttg cttaactgaa ccagttgcag cacgtcttct
    46981 tcgctgacag tttttcctcc ggatttttgc agcacttccc gcgccgcatc gctatgggtg
    47041 gcgatcaccg gcacaccaat cgatagcgcc tcacacaaaa tcagcgggta gttatcgaca
    47101 cgagaactga acaccagcgc atccatctga ttgagcgcgc tcatcaactt gcgcttgtca
    47161 gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt accaaaggta
    47221 tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg gttagttttg
    47281 ccgtcgtaac gcaggtcatg cgccaccacc gcgattttcg gcttgccctg ggtttcgcgc
    47341 accggaggca gatccgccag aatcgcttcg gttgccatat caatgccatt attgataatc
    47401 cggcaacgcc ctggaccgta caggctattg aaagcatcag ccacatgctg gctgggggaa
    47461 ataaactgac agcccagcgc cagcatctca cgaaataact ggcgtttgcc cgccaccagc
    47521 tggtgcgcgc gatcaatctt caccggcgga tagttattta aggtcgggca tttctggcaa
    47581 cccgttttcc agccttcgca accgtcggta aaggcgcagc gcccggttac actccagtga
    47641 tcgtgcagtg tccagaccac agtgacgtcc ggtttatggt ttttcacttt ttcgcaaaag
    47701 cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg caggaccacc
    47761 ggacccggtg tgcgagtaat ggtgcgatat aactcattga aattgccaaa cagatcgcga
    47821 ttaaacagac gaaacagtgc aatgttcgcc atcgcggtca tccgcggcgt atgtttgatt
    47881 acctgcggat agttttgatg gctgacgctc tctttgccgc ctttgccgta gccgtagaca
    47941 aaatgtgacg ccagcccctg ttgcagcgca cgctggtgga gatctaacgc cacacctgcc
    48001 gctccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt accttcactc
    48061 gcgctttttc tcccaccacc agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg
    48121 cgcccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg ttggcaccga
    48181 gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga ttgccgatag
    48241 tgacgccgtg gcgaatggta aaatcatccc ccgcaaccac gtttttattg atcactacgg
    48301 cgtaaccgtg atggatagta aagcggcggc caatggttgc ggctgcctgg atttcataac
    48361 cgaaaaagca ttcggtgata atgcgataca gcaccagcag cggtgccgcc cacagattgt
    48421 tgaggacgtt ctttttgcgc cacaccgaac aaaaatgagc aacacgatag gcaagaacca
    48481 tgcagcacgg gcgtaaactc cagctgttgg cgcgcagatc ttccagcatt tttagcgccc
    48541 ccgaatacca tcagccagac gtttgccgtt acgcaccgac agcagcgtca acaaggtgcg
    48601 ccaggtcatg cgtttattac ggatctggta gagggtaaac agctgatatt ttttgcttgc
    48661 acggtcaaat ttatctttgt gctttcggta aaagtggaag tacccggaga attttttcgg
    48721 tgacgaggtg atctgcattt caccgtgatt gatatgcagg atctgcgtcg cttcttccac
    48781 tttccacggt tcgccgtact ccaccaccat ccgcaggaag atgtcgtaat cctgtgctgc
    48841 tttcagttcg gtatcgaaca aacactcttt gaaacgccat gcccaagtaa agacctgatt
    48901 gccaatgata ttgcgtttat agaacaggcg gcgtgaatac ggcgatttgg gatatagcgg
    48961 caaacttgct ggttgggaat agacttcgcc ctggcaaacg tagtcgttag cgtacaaaaa
    49021 ggcgtgagtg accagttgct gtttatgggc gaggaagacg ctcagacggt tgggtgtcca
    49081 ttcgtcatcg tcatcaatcc cggtgatgta ttccccttgc gccagcataa tcgcctggtt
    49141 acgtaccgcg cacgccccgc tgttaatgtc gttgtgaatg taggtgatac gcggatcgtt
    49201 gagggcagtg acgtactgtt gcagttgttc ccaggaggtg gagcaatcat ccacgatgat
    49261 catctcccag ttgctgtagt cctggcgcag gaccgatttt atcgcccgaa tcgccagttg
    49321 ttggcggttc caggtcggca tatagattga aatcagcgga ttgtctttca taggttgttc
    49381 tcccggagtg tcgagatgtt ctgccggttt ataagcttgt ttacgggttc tgttgccgga
    49441 tgcggcgtga gcgccttatc cggcctacgg agctggtttg cttgccggat gcggcgtgaa
    49501 ggtaggtcgg ataagatgcg tcagcatcgc atccgacagc ggatgtcgtt atttcgcatc
    49561 cgacttatat tcgtattcgt aatagccata atcctgatac gcgctggcgc ggcggaagat
    49621 ggagttcaga atcacccctt tcaccggaat accattttgc tcaaagcggc tcagactggt
    49681 ttccacttct ttcaatgtgt tgaccgcata acgcgccacc attaacgtgg ttccaacatg
    49741 acgaccaaca attgccgcat cggtcactgc cagaatcggc ggcgtatcaa tcaacaccag
    49801 gtcatagttt ttactcgccc agttcaccag ttcggcaaag cgttcgctca tcaacagttc
    49861 agaaggattt ggcggcacct gaccgcgcgg gatcaggtca aatttggcaa tagaggtcgg
    49921 tttagcagcg gtagtaatat cgccctgacc aatcagaatt tccgacaggc cattaacgtt
    49981 attagtgccc agcagctcgt gggtgtagcc tttgcgcata tcgcagtcga tcaacaacac
    50041 ccgtttattg gtctggctga tcaccgccgc caggttggcg cagacaaagg ttttaccgat
    50101 tgacgggcta accccggtca tcatcaacac attgttctgt gcctgcatca tcgcgaagtg
    50161 caaactggta cgcagactgc ggatggcttc aatcgccaga tcggttggat tccccatcgc
    50221 cagtagctgg ctctgtttat agcgtttaat ccctttgatg gttttgacgc tatcgcgcgc
    50281 tttctgccat tccgaaagcg ggatgctggc atagacgctg ataccgtgtt cttccagcac
    50341 ctgcgggctt tcgatgccgc gattaaacaa cgagcgcagc agcacaccca cgatagaaag
    50401 catcaggccg aggataatcg ccccaaggat aatcagctct ttcttcggtt tcagtacacc
    50461 tggctgggtg attgccgggt caacaatgcg cacatcgccg acggtgctgg cctcggtgat
    50521 tttcagctcc tgctctttat tcagcagttg catatagacc tgctgaccag actcgacatc
    50581 gcgggtcaga cggacaatct cctgctgggt tttcggcatc gccgttacgc gaccgttaag
    50641 tttggctttt tcgtcttcca gcgcctgacg tttctccagc agcgtgcggt acgccgggtg
    50701 aactttggtg tacagcttgg agatttccgc ctctttaaag gtcagttcgt tcaactgggc
    50761 gtcgatgttc accatcgaat cgagcaccgc tttcgcttcc agcggcagat caacagaatc
    50821 tttatcctga cggaaggcat tcagtttgtt ttcggcaaca tcaaggcggc tacgtacttc
    50881 cggtaactgt tgcgcgagga aagcgaggct tttcgacgct tccgccgatt tgcgctcaat
    50941 attttgttcc tgatagttac gggcgatgct gttaagaatg tcgcggatct gttcgcgatc
    51001 ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg ccgttctccg ttaccgtcag
    51061 gctgttttgc agttgattga tcatccccag cgtggagtat ttggtgacgg taaactcact
    51121 gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg ccttcttttt tcagcatctg
    51181 gcccgcttgt ccacgggcgc taaagccgcc atcactgctc agggtgtagt ttttatcgtc
    51241 cagcacatta agcgtaaaca cctgatccgc catctctttc gggcggttaa aggtggtcac
    51301 tttcaccgtc tcgttctgac gtcccatcaa acgatcccag cccgcaccga aaatcgggaa
    51361 cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc accgttttac caagcaccag
    51421 acgcgaacga atcaactgga tctcggcgtc cgatgcaggc ggtttgttgg ctaatgccga
    51481 accaatgtcc tgcactaacg aattgccgct gttttgctcg atttgtacca gtgcgtcggc
    51541 actataaatc ggcgtggcga agaaggtgta aaccacggca cagagggcaa atacagcggt
    51601 gatgccaatc acccaccagc gcgcttcaat gacggtgccg accaggcgac caatatcgat
    51661 ttcatcactg cccgttaccg gagcggcatg ttgttttact ttttctgtca ttcttatacc
    51721 tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc gttcaagtaa tgtgtacacc
    51781 gctgcaaacg tttcccggct tttgcgatac ggatcgggga tttcacattc gttatcccag
    51841 tgaccaaaca gcatcacttt gccgcgcatc tcaggtgcca tctcgcataa gcgttcgata
    51901 tggcgctttt ccatggtcaa aatcaggtcg tagttgcgac acagacggcg gctgatttgc
    51961 cgtgcacagt gaccttccag agacagttga tgttctgcgg cgatgctgat agcagtaggg
    52021 tcagcgccct taccgaccag cgcgccgagt ccagcggact ccactttcag ctccgggtga
    52081 taacgttgca gtaagcgttc cgccgtcggg gagcggcaaa tattgccgac acagacaact
    52141 aagatgttgt taaacatgac gattaccagt tatgaatgtc gctggctgta tccgtcatgt
    52201 aacggacacc gctaatagtt ggcagcaact gattgatcag acggttccag cgggaaaccg
    52261 gggcggtggt gacatacacc acgtcataag gttgcaggcg gaattctgtc gccatcacca
    52321 gcgacgtggc atcggacata tccagctggt agatattggc aatcttgccg ttacgcccgc
    52381 cctcgccttt cagcggacga atgacaaaga tgccgctggc gttggaggtg gtcatgtcga
    52441 taccttcagc attacccagg gcttcagtca gggtcatgcc gctaaagtcc attttcaggg
    52501 tgctctgttt cttcacttca cccatcacaa atactttcag atcatcatta cgcggcacgt
    52561 agagaatatc gccgggataa agcagtcggt tctgattgag gtcgccgttt tgcatcagca
    52621 cctgcaaaga aatgcgttct tcacgaccat tgtgtgttag caccacgttg cgccagtcag
    52681 cggtgtcggt caggccacct gcggcgttga tggcgtcgag aatggtcagt ggtacgttgg
    52741 tgatcgcctg ttgaccggat ttattcacct gacctgagat ataggtcttt tgtgagcgga
    52801 aggcggcgat attaacgtcc acctgcgggt cagcgatgta cgtcgctaag cgcccggtaa
    52861 tatcactgcg gatttcagcg agcgttttcc cgactacgtg aaccttgccg atatacgggt
    52921 aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc gctggagctg cggtactgac
    52981 cagctggcgt ggtgagttcc gggtgatccc agacggtgac attaagaacg tcccccggcc
    53041 cgacgcgata ctggtaattc gcgatctcgc tttccagcgt catattgggg cgcgctacat
    53101 tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag cggataaaca ttcaccattt
    53161 tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc tttgcccatc gtcgacatat
    53221 tgctgcccgg aagtactgtg caaccgctta tcaaggttac tgacaccaat aatggcatca
    53281 atttcatttt ggatttcatc attgtttatt tatcactttg gcagagtaat tatcctgtgc
    53341 actattaata gcaatgtcgc catgcacatt taccttgcag ttaattgaat acaaatttaa
    53401 ctggcatcag tcctaaaaaa attgatttca tccgcaggct attgacagaa taattcagac
    53461 tggtctttca ggcatccaga cacgctaccg cccctggctt tttagccacc aatacactga
    53521 tttagcttaa ttttttaaac cctctctgca tacaatcgtt gatgagaaag ggttattgcg
    53581 gaaattaact tccgaatata aggtgacatt atggtaattg aatattggct ctccaataat
    53641 gcaggaggaa gtgttacagc taacggaata gcaggcaagg taacaattcg gcaattggct
    53701 atttttaaga attatattaa gtgtcattca atatggtttt taggagtttc tttaggttga
    53761 caatatttaa tattgcgcct ccgcatgcga tatttcttaa ataatgtttt attattacca
    53821 cttttaattc agggataatg tgaggttatt accctcaaat aatatgagat aaatagtgct
    53881 gcaacattgc atttttgccc cgatttatcc atgatcgaat tgtgacattt gtcatacaac
    53941 gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg gccgggttaa
    54001 tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt attgccatcc
    54061 tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg ggttaccggg ctgttgctgg
    54121 cgatgttaat gcgcctgtta ctgctggcgt caatctcctg gctggtcacc ctgacgcaac
    54181 cgctgttcag cttccgctcg tttaccttta gcgcccgcga cttaatcatg ctgtttggtg
    54241 gtttcttcct gttgttcaaa gccacgatgg agctgaacga acggctggaa gggaaagaca
    54301 gcaataatcc cacacaacgc aaaggtgcga agttctgggg ggtggtgaca caaattgtgg
    54361 tactggacgc catcttctca ctcgactcag tgattaccgc cgtcgggatg gtcgaccatt
    54421 tactggtcat gatggccgcc gtggttatcg ctatcagcct gatgttgatg gccagcaagc
    54481 cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgctta agcttcctgt
    54541 tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg aaaggctacc
    54601 tgtacgctgc cattggtttc tcggtaatga tcgaggcgct caatcagttg gctatcttta
    54661 accggcgacg ttttctttcc gctaaccaga cgctacgcca gcggactacc gaagcggtaa
    54721 tgcgcctgct tagcgggcaa aaagaagatg cggaactgga cgccgaaacc gcgtccatgc
    54781 tggtggatca tggtaaccag cagatcttta atccgcagga acggcggatg attgagcggg
    54841 tacttaatct taaccagcgt actgtcagca gcattatgac gtcgcgccac gatattgagc
    54901 atatcgatct caacgcgccg gaagacgaga tccgccagtt gctggagcga aatcagcata
    54961 cgcggctggt ggttaccgac ggtgatgacg cagaagattt gctcggtgtt gttcacgtta
    55021 tcgacctgct acaacagtca ctgcgtggcg aaccgctcaa cctgcgggtg ttgattcgcc
    55081 agccgctagt gttcccggaa accttgccgt tgttacctgc cctggagcag ttccgtaatg
    55141 cccgcacaca ctttgctttt gtggtggatg agtttggctc ggtggaaggg attgtgacat
    55201 taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa gagatcgacg
    55261 cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt catatgccgc
    55321 tggaagatct ggtgcaatat gtgccgctgc cgctggatga aaaacgtgaa tatcacacca
    55381 ttgccgggct gttgatggaa tatttacagc gtattccaaa acctggcgaa gaagttcagg
    55441 tgggggatta tttgcttaaa acgttgcagg tggaaagcca tcgcgtgcag aaggtgcaga
    55501 ttattccgct gcataaggat ggcgagttgg agtacgaggt gtgatggtat cggtcatttt
    55561 acccaatagc cgaaaggaat aagatgaggg aaccgcgcca gcggcgattt accgccggga
    55621 gcccgggttg ccagggtggt ggcgattgag cccccctggc acgttcaccg gcttgagcgt
    55681 tccatagtag cgaggaacat caagtgaacg gaacagcctt cgatcttaca tattcagaat
    55741 ataatgatgc cgccacgaac gcctgatgcg acgctttacg cgtcttatca ggcctacagg
    55801 acaggtcacc aggtaaccta catcttctcc agcaacttct tcacatcttt gccattgcgg
    55861 gaatctttat tccgctcggc ccagtcattc agacgacgtt tcgcttcgtc ctgtagatgt
    55921 ttgcgcagta actgatccac ttgcagactg taattgagtt gctgccagtt gccataaacc
    55981 cgcagcggta ctggcgtttc tttaaggaaa tcaatcagtt tgctttcccc gttccagcca
    56041 cccacgaccc gaatatcaaa ctgggtgtcg caggtttgct ccgccagatt caacgtacca
    56101 gccccggaaa gcgccagcac aggcgattga ccttgcatgt cgtttaacgt cacgacgcca
    56161 tccttcaacg tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc gaagttttca
    56221 gcggccttca catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc
    56281 atcccttcca tgcgcgtgtc ggtcatttcg acatgtgcct gtccttgcca gttgtggcgg
    56341 aatgcgtcgg catctatgtc agcaccggag aaatcaccag ccagtgacat ctttccggtc
    56401 aacgaaatcg gatagttaaa cgccttcaga atggtgccaa tctcaacgtt ttccagccgt
    56461 ggctggaagt ttatccgcgg atttattgat gtcgcgtcca gcgtgcccgg cagtgaaacc
    56521 tgtccaccgt taagcttgcc ctgcagttga gtaatttcca gcaaacccga cttgttggtc
    56581 atttgcgtgg caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac
    56641 aaaatatcag ccgtaaagcc ttgcagtccc tgatatgccg gttcatcaat acgcgaagaa
    56701 atgaccgggc gcggcaacgt tgattggctc tgcccctgct gcgcggcacc gttttcacca
    56761 ttcgctgttt cattaagcgg gatgaggttg tcaagattca gttgcgggaa ttgcagtctc
    56821 agctgccatt ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag cgtactgtca
    56881 ttggcggtca aactaatttg gttaaatgaa aggcgtttat gtgactcctg ccactgggcc
    56941 tggaaactcc cctgcccatg aataccttgt tttggtaaat ctgcaccctg caactgccag
    57001 ttaatttgtt caatagccgc cgttaaatca tgcggataat cagaagcatc taccgtaccg
    57061 ttaagggata tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa ctcaaatgaa
    57121 ccacgatgtt ggggatcttg ttccatttgc aggcgaatat tgcggattgt cacctgctcg
    57181 tcatcttcat gctggaacac cagcacgctg tccgccacct taagactgga tatatcaaac
    57241 gaccatccgc gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg
    57301 tcttcactgc gcaccacttc cgtctgcggc gtcagttgga tcactgcccc ttttagcatc
    57361 acctgcttaa cgctcagttg atgactcagt agtggtaaaa gcgccacgtc cagacgcatg
    57421 ttgtcggcgc gaaccagtgg ctggctcgcg ccctgggcgg tgagagacat tcgcccggag
    57481 aggatactaa gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa
    57541 ccgctacgtg cagcaacttg cttgaccata tagttgcgga aatcattcgg attcaccagc
    57601 aacactaacg cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga
    57661 aatcgtctca tggtatcctc aatgggcctg aattagtctt tatcgattcg gctggctacc
    57721 gcgccctgct ggttgcgata tttcgcatct tcacggcggt tgtaaggtcg cgccgccggg
    57781 ccggaaagcg gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc
    57841 agcttaccgg agttgtagaa ctccagcaca atgcaaccag accagcccgg atcgatacgg
    57901 tgcgcggtga cgtgcaccat cagccccaga cgcgccaaag aggaacgccc gtccagccag
    57961 cccaccagat cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagttctcct
    58021 gggtgaagat agaacgcctc gccctcgtcg agaacgatct catcgctcat cacgcggtca
    58081 agcgcggcgc tcacttcatc tttgggaccg ctcagatcga taaacgctgc cgtgtgacca
    58141 cggaaggtac gaaatttatt gcccaggcgt acatccaccg tcgcgccgtt aatacgctcc
    58201 actggcggac gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg
    58261 tcacacagac gcatggcatt ttctcctttc gcgcatcact cccttaacgc caattacgtc
    58321 aagggcatac caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg
    58381 acttattttc gctttcaata tatcgatcgc gatgcggttt ttcccgccgc gcggcacgat
    58441 aatgtccgca tattgtttag aaggctcaat gaattgcagg aacatcgggc gcacggtttt
    58501 ttgatattgc gccatcacgg aatccattga acgcccacgc tcgttaacgt cacgcttgat
    58561 gcggcgcatc aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcgtc
    58621 acgcaaacgc gcatccgtca gcaacaaaat gccttcgaga atgatgacct tcttcggctc
    58681 aaccgtcacc gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat
    58741 tgccgagccg cgtttcaacg cttgtaaatg ctcaagcagc aggttgtgat ccatcgcgct
    58801 gggatggtcg tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta
    58861 atagcagtct tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gtaattcacg
    58921 ataaagggta ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat
    58981 gacgcactga tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg
    59041 aagggggcga cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc tgataccagt
    59101 cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacaa agaattcccg gtggcaaatt
    59161 acgttgatca gttttatgta aggtaaaaaa tgttatacgc agttgcgcaa attatccgcc
    59221 tttacgtcac tttatgagca attcacatat aaaatgtaaa acttttgtac tagcataaac
    59281 acagaaacga atactggcga cctggtcttg cggataaagc ggtaatgagc aaacaatcac
    59341 agcatgtatt aattgccctg ccccacccac tgcttcacct ggtcagttta ggtttagtct
    59401 cgtttatctt tacccttttc tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac
    59461 tgtggttccc gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc
    59521 cgggaattgc gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca
    59581 ccagctcgct gaacatgacc tggacgacca tcaatattgt tgaagccgtg gtcggggcag
    59641 tgctgctacg taaattgctg ccgtggtata accccttgca aaatctggct gactggctgc
    59701 gtctggcatt cggcagcgcc attgttccgc ctctgttagg gggtgttctg gttgtcctgc
    59761 tgacgcccgg agacgatcct ctcagggcat ttttgatatg ggtactgtca gaatccatcg
    59821 gcgctctggt actggtgccg ctgggattgt tatttaagcc acactatctg ctgcgccatc
    59881 gcaacccacg gttacttttt gagtcactgc tcacattagc catcacgctg acgttaagct
    59941 ggctttctat gttgtacctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga
    60001 gcgccgtgcg cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg
    60061 tgtcgctgat gatggccgcg gatccctccc tgcttgctac gccgcgtacg tacctgatga
    60121 gccatatgcc gtggctactg tttttgctga tcctgctgcc cgccaacatc atgaccatgg
    60181 tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga
    60241 acgcgatgga atattccgct atcggtatgg cgttagtggg caccgaggga caatggctgc
    60301 aaaccaacaa agcgctctgc cagtttctcg ggtacagtca ggaagagctg cgcggactca
    60361 cctttcagca actgacctgg ccggaggatc tcaataaaga tctccaacag gttgaaaagc
    60421 tgataagcgg tgaaataaac acctattcaa tggaaaaacg ctactacaac cgtaatggcg
    60481 atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca cacggatggc acgccgctct
    60541 attttatcgc tcagattgaa gacattaacg agctaaaacg caccgaacag gtgaatcagc
    60601 aactgatgga gcgcatcacg ctggctaacg aagcgggcgg gattggcatc tgggagtggg
    60661 agctgaagcc gaatattttt cgagctgtat gaaattcctc cgcatatcaa accgaactgg
    60721 caggtgtggt acgagtgcgt gctgccggaa gatcgccagc atgccgaaaa agtgattcgt
    60781 gattcgttgc aatcacgctc gccctttaag ctggaatttc gcattaccgt aaaagacggt
    60841 attcgccata tccgcgccct cgccaaccgg gtactgaata aagaaggcga agtcgaacgc
    60901 ctgctcggca ttaatatgga tatgaccgag gttaaacagc ttaacgaggc attgtttcag
    60961 gaaaaagagc gcctgcacat tacgctggat tccatcggtg aagccgtggt ctgtattgat
    61021 atggcgatga aaattacctt tatgaatcca gtggcggaga agatgagcgg ctggacgcag
    61081 gaagaagcat taggtgttcc gctcctgacg gtgttgcata ttacttttgg cgacaacgga
    61141 ccattaatgg agaacattta cagtgccgac acctcacgtt ccgcgattga acaagatgtg
    61201 gtgttgcact gccggaacgg cggcagttac gacgtgcatt acagtattac gccgttaagt
    61261 actctggacg gcagcaatat aggctcggta ctggtgattc aggacgtcac cgaatcacgc
    61321 aaaatgctgc gccagctgag ctacagcgcc tcccatgatg cactgacgca tctcgccaat
    61381 cgcgccagtt ttgagaagca actgcgtatc ctgctgcaaa cggtaaacag tacacatcag
    61441 cgacatgccc tggtgtttat cgatcttgat cgctttaaag cggtgaatga cagcgccggg
    61501 catgcggcgg gtgacgcttt actgcgcgaa ctggcgtcgt tgatgctgag tatgctgcgc
    61561 tccagcgacg tgctggcgcg actcggtggt gatgaatttg gtctactgtt gccagactgt
    61621 aatatcgaaa gtgcgcgttt tatcgctaca cgcattatca gtgccgtgaa tgattatcac
    61681 tttatatggg aaggccgtgt gcatcgggta ggtgccagtg ccgggattac cttgattgat
    61741 gacaacaatc atcaggcggc agaagtgatg tcgcaggctg atatcgcctg ttatgcctcc
    61801 aaaaatggtg gacggggccg ggtgacggtt tacgaaccac agcaagctgc cgcacatagc
    61861 gaacgggcgg cgatgtcgct tgatgaacag tggcggatga ttaaagagaa tcagttgatg
    61921 atgatcgccc acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt gtggctgatt
    61981 tcacttaagc tctggagttg cgaaggcgag attattgatg aacaaaaatt tcgtcgtagc
    62041 ttcagcgatc cggcacttag ccatgctctt gaccgacggg tattccacga atttttccag
    62101 caggccgcaa aagcggttgc cagtaaaggc ataagcatcg ccctccccct ttccgttgcc
    62161 ggtttgagta gcgccacgct ggtgaatgat ctgcttgagc agctgggaaa tagccctcta
    62221 ccaccacggt tattacatct gattattccg gctgaagcga ttttagatca cgcagaaagc
    62281 gtgcaaaaac tgcggctggt gggatgtcgg atagtgctca gccaggtggg ccgcgatctg
    62341 caaatcttca actcgctgaa agcgaatatg gcagattacc tgctacttga tggtgagtta
    62401 tgcgccaacg tgcagggtaa tttgatggat gagatgctga ttacgattat tcaggggcac
    62461 gctcagcgac tcgggatgaa aaccatcgcc gggccagtcg ttttaccctt agtgatggat
    62521 acgctttctg gcatcggcgt cgatctgatt tatggtgatg tgattgccga tgcccaaccg
    62581 ctggatttgc tggtgaatag cagttatttc gcgattaact gagagagcgt ttgcccattg
    62641 cctgatgcga cgtatcaggc ctacaattcg tccgttccgt ctggttgcca gccttccgta
    62701 taccagatat gcaacagcgc ataagaacgc caaggcttcc agcgctcggc ataacggcgg
    62761 atttgtgccg gtgtcatccc cggaaatcgc tgtttaatca gataatcatc cggcagaaaa
    62821 acatctttcg cctgccagcc acgcaaagcg aaataattcg ccgtccagcg cccgataccc
    62881 ggaaaagttt gcagcgtttt catcgcctgc tccacatcgc ccggaattgt cattggtaag
    62941 ctgccctcca gcgccgcatt tgccagatga atcagtgcct ctgcccgttt caacggcatg
    63001 cctaacgctt ttaatgcctg cgggtcggct gctgccagcc gctgaggcgt ggggaagcag
    63061 atatactccg gaaaatcatc cagccgttcg ccataaagct gtacaacttt ggcagtcaat
    63121 tttgccgcca tcgccacgct caccagttgg cctaaaatcg cccgcacacc ctgctcaaaa
    63181 gcatcaatac agccgggtaa acgcaatccg ggccgcgccg cgcctaactt gcccaacgca
    63241 ccgttaacaa tctgcgggtt acattgcaga tcaaacaggc ggctcatttt cgccagacac
    63301 tcagcggcaa cgggttctaa acctgcactt aaatttatgt gcagagtatg gcgggctata
    63361 tccggaatag cagtcaccac gccgcgatat tcgcccaccg ccagactacg ggcataataa
    63421 tcgtcagcga ccgtttccac accactcact gcacgggcgg cgagaaatcc caacatccac
    63481 gaccagtcat acggcggctg ccagttcagg gtatacatcg catctcctta ttcatccgct
    63541 ttcagcataa acgttattca gacgctacgc tttgctttca tattccggtt gtcgcgacgg
    63601 caacatttcg ctaaagtcac gccccttctt caccggcatg gggattattt cgtgtttatt
    63661 ggttttgatt acggtacagc aaactgttca gtggcggtca tgcgtgacgg taaaccgcac
    63721 ttgctaaaaa tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt
    63781 gaagcggtaa gcgaatggct gtaccgccat catgacgttc cggcagacga cgatgaaacg
    63841 caggcgttgt tacgacgggc gattcgttat aaccgcgaag aagatatcga tgttacagcg
    63901 aaaagcgtgc agttcggtct ttcctcactg gcacagtaca ttgatgatcc agaagaagtg
    63961 tggtttgtga aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta
    64021 gcgctgtttg aggatctggt ctgcgcaatg atgttgcaca ttcgccagca ggcgcaggcg
    64081 cagctgccag aagcgattac tcaggcggtg attggtcgcc cgatcaactt ccaggggctg
    64141 ggcggcgatg aagcaaacgc ccaggcgcaa ggaattctgg aacgcgcggc gaagcgtgcc
    64201 ggattcaggg acgtggtatt ccagtatgag ccggtcgcgg ctgggctgga ttacgaagcc
    64261 accttgcagg aagaaaaacg ggtgctggtg gtggatatcg gtggtggtac gactgactgt
    64321 tcattgctgc tgatgggacc gcagtggcgt tcgcgtctcg atcgtgaagc cagcctgctg
    64381 ggtcacagtg gttgtcgtat tggcggtaac gatctggata tcgccctggc gtttaaaaac
    64441 ctgatgccac tgctgggtat gggtggcgaa accgaaaaag gcatcgccct gccgatcctg
    64501 ccgtggtgga atgcggttgc catcaacgac gtacctgcgc agagtgattt ctacagtagt
    64561 gccaacggtc gtctgcttaa cgatctggta cgcgatgccc gcgaaccgga aaaagtggcc
    64621 ctgttacaga aagtctggcg tcagcgttta agctatcgcc tggtacgcag cgcagaagag
    64681 agcaaaatcg ccctttcaaa cgctgcggaa acccgcgcct cactgccgtt tatcagcgat
    64741 gaactggcta cgctgattag ccagcaaggg ctggaaagcg ccctcagcca gccactggcg
    64801 cggattctgg aacaggtgca actggcgctg gataacgccc aggaaaaacc ggacgttata
    64861 tacctgaccg gcggtagcgc ccgttcgccg ctgattaaaa aggctctggc agaacagttg
    64921 ccgggcattt cgattgcagg cggcgatgac tttggctccg tcaccgccgg gctggcacgc
    64981 tgggcggaag tggtatttcg ttaaaagaaa atggctcagc agcggcttaa atctgcctgc
    65041 gcctgagcca gtttttctgc cgcctgacgt aacggttcca tcaacggcat ggtccgtgct
    65101 ggagcataaa ggcggaatcg ctgcaactct gtcgcgccga cagtcaacgc cgattccagc
    65161 cgatgtcgtt tggcggccat taccgcctgt ctgtctgctg gcgttaccgc ttcgttcggc
    65221 ctgaacacaa aacggcggtc acagctcgct ttccagtcga taaccgcctg ggtcagatgg
    65281 tcgccaaacc ctttaacttg cttaacgcca cgacgggtaa catccgctgc tgtttcaata
    65341 ccaaaagacc gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaaaaaat
    65401 ccctccagaa acttctgctt ctgccgttcc cttgcggtgt cataaagtgc tgccagagcg
    65461 cgtttctcct cttccggtaa cccgagaatt tcgtccttca ttttttcgag catcgcccgt
    65521 ttggcgataa aaccttccag cccgcccaac tgttggatct ggctgactaa atgctcatag
    65581 tccattttcg cgcgattaaa tgcctcacgg cgttgctgga tttctgcttt gtacgctttg
    65641 cttgtcagac tgccaataat ccagatagcc gacagcacag gaaccaaaat aatataacgc
    65701 ggttctgcct gaaggccgca aagcaacagc gataacgctg acagtgcgat ctcaatcaga
    65761 atgatgtatt cgcgccgtaa caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga
    65821 tcgggtaatg gcaattgcaa tgctggcggt gctactgacg ccatcaccat cgcccagact
    65881 ttcgccagca caaaatcacc gctggtggta atgacctctt caccgagatc aacaaaatag
    65941 ataacgcctt gattatccag cgtacaccac gggcagtcgg tcaaatgagc gggataaaca
    66001 tgcattgccg aaacgggaca tttctttaac tgttggcgca gagaatcaag tgccgctacc
    66061 cacgctttag ccgtcggacg cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac
    66121 atggcttcaa catcactcgg taacatcgat agcggaatcg atcgtggcgg cggttttaaa
    66181 ccacgtcgct gattatctga cgcgtaggca taacggaaat gggtaatatc cgtctccagc
    66241 gcattacccg catcagagat aagcggcaca ccagaataag gatgccggcc accaaacaag
    66301 acgtgaaaaa tcagcaacgc aaggccaaaa ttatcgtgat tttctgtgcg ttcaaagccg
    66361 acaaatgacg acaaggtttg tagctctggc ggcgtaaaat gcgacacgcc gacttcgcat
    66421 aaatgcagtg tgccattgac gttaatctga aaggagtcgc tatcaatcag taccactttg
    66481 ctgtcgcgac ctaccataaa actgttctgg tttacgtcac ccacgacgtg cccgtgctcg
    66541 tgaaccgtag caaaagatga agcaatattg cgcgcaacat agagtagaaa atcccacgca
    66601 caatgagggt aactctggcg acgatgtgcc gggctataga tcatatgaat cggttcttta
    66661 ccagaaactt ttggcatcat aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt
    66721 gcctgcggcc aggcgacata attcaacaac tgcgcgtcag ctgtcgcagc cataaaggca
    66781 agtttgtcct gttttgaggc gggtggcggc gtgtgataaa tcttggcgac gctatcgaca
    66841 aactcctcga tatcataaac cgcgccttca ccgcctttgc ccagttcacg gcctggcgta
    66901 acgcactcac cagtagcagt atataaagtg ggtttcatgg taaccacagc gccagtgcca
    66961 acgttttatc atcatccgta cgctcgttta ccgccgggct ggacaaaaac tgctttaaca
    67021 attcaggtaa taaatcgagt tgttcctgcg ttactgacgc cagtccattg aaaaacgggg
    67081 taaagaaagg cacatgagga gaattatcca gcatatttaa cgccagccgc tgaataccat
    67141 ccgtaaatgc tgcaacttta tgcgcacgcc cagtgctggt gaaagtttcc agtctggaaa
    67201 cggcatcttc gtcggtaatg aagtgcgtca tgttggcata ttcacccacc atcggcgtga
    67261 gaggcaattg cagaccgtga cccaaatcta ccaccacgcc gccatcgcca atctgcataa
    67321 tcaaggtgcc gtcaggcgat gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg
    67381 ctaatgcttt ggcttctgcc tcggcaaaaa gtcgttgacg aatagtcagt accatatttg
    67441 tggcgaggac atcattaagc cccaattcac cgtcctgtac tttttgcacc ataaacgcca
    67501 tcgcttcatt gacggcgagc gtcgcgcctt ctcctccctg cgaaacgcta cctgcgccat
    67561 cagcaaggaa catcaccagt aatggttgct ggtcgtttaa ccagacaacc tgcatctggc
    67621 aggcatcctg acaaggcaaa tcagcactaa tgtgcgatgt tccaacagcg gatgcataaa
    67681 ccagacgcca gctcacactg acgtccagcc ttttggcgct tccagcacaa cttccgttcc
    67741 cggcgtggag cgggaaaccg aacgcagcga actggataac cagctaaaca gttcacggaa
    67801 ttgtaatccc tgcagtggca aaggctgacg aacgctgatt tgtgccagag tcttcatatc
    67861 cgccccctga acaccgatgg aaaagaaggc aaacttctta tcttcttccc cctgaaacac
    67921 tttgttggcg gcagcctgcc actcatcggt tggagcgcca tcggtgatca ggaaaatcca
    67981 cgggcgataa tacgaaatac catttgcgcg gtactcacgt ttcctctctt caaccatatc
    68041 tagggctttg gtaatggcgg cccccatagg cgtatccccc tgggcaaaca ggatgggcgg
    68101 gaaaaagtta gcggcgctgg taaagggttg ttccacatgc accgggccga acgtcacaat
    68161 gccgagttcc actcttttta aagccagcgg atcagcaagc agttcatcac gaaaggtaac
    68221 caatccggcg ttaagttcat taataggtct gccgctcatc gagccagaaa catccagcag
    68281 caaaatgcag gggcaacgtg gttccgggtt actggcaaaa tcgctggtgg caaacgtgat
    68341 ttgttcgctc ataagaaatc ctttcttaga gaattatcga ggtccggtat cataccgatt
    68401 gcaataatga aataagagca aacgaagaaa ctttcagaat taggcccgtc ggcgcagaaa
    68461 attttgctat tcgcctcgca agaacggaga gccgtcgatt gacactaagg gcggagtgac
    68521 ataatttcag gagtgagggt tagggagagg tttccccctc cccctggtgt tcttagtaag
    68581 cctggaagct aatcactaag agtatcacca gtatgatgac gtgcttcatc ataacccttt
    68641 ccttattaaa agccctcttc tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc
    68701 atcggcttac ctcctttcgc cctgaatgca gtctatcgct aacgcgtgaa taaaatagtt
    68761 tcctctgtga ttattgcgta acgactcgca aagaatattt gctggcagga tcgcagacta
    68821 caaagcctac ggattgacaa tcttatcgtg aaggcatact ttcaggagtg agggtagagc
    68881 ggggtttccc ccgccctggt agtcttagta agcggggaag cttatgacta agagcaccac
    68941 gatgatgagt agcttcatca tgaccctttc cttatttaag gccccttcct cgggaggggc
    69001 tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc tgtatgcagt
    69061 ctatcgctga cttgcggata aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa
    69121 gaatatttgc tggcaggatc gcagactgca aagcctgcag attgacaatc ttatcgtgaa
    69181 ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag tcttagtaag
    69241 ctggccagct aatgactaag agcaccacga tgatgagtag cttcatcatg accctttcct
    69301 tatttatggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct gaaatcggcg
    69361 gcttacctcc tttcgccatg caagcagtct atccctaacg cgtagataaa atagtttcct
    69421 gtgttattac tggatgcgtg ctcgcaaatg tgcccgtcat tcagacaatt cccgacagtg
    69481 tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc attccgcgaa
    69541 acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc
    69601 gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt tctggcaagg ccgcaatgac
    69661 tcccggagtg cagccccagg ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc
    69721 cgtggtatgc gttccggccc attagccccg gttcaggcgg cgaccgccgt agaacaggca
    69781 gttccgcgtt acctcaccgg gcttggcacc attatcgccg ctaatactgt tacggtgcgc
    69841 agccgcgtgg acggtcaact gatggcgtta catttccagg aaggccagca ggtcaaagca
    69901 ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc acaaacccag
    69961 ggccaactgg caaaagataa agccacgctt gctaacgccc gccgtgatct ggcgcgttat
    70021 cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc tggatgccca acaggcgctg
    70081 gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag cgcgcagttg
    70141 caactcgact ggagccgcat caccgcacca gtcgatggtc gcgttggtct caagcaggtt
    70201 gatgttggta accaaatctc cagtggtgat accaccggaa ttgtggtgat cacccagacg
    70261 catcctatcg atttagtctt taccctgccg gaaagcgata tcgctactgt agtgcaggcg
    70321 caaaaagccg gaaaaccgct ggtggtagaa gcctgggatc gcaccaattc gaagaaatta
    70381 agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg tatgattaaa
    70441 gtgaaagcac gctttaataa tcagaatgat gtgctgtttc ccaatcagtt tgttaacgcg
    70501 cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg
    70561 ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa acaaggtcag caaacatctg
    70621 gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc
    70681 gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg
    70741 gaagcccaga gcgccaccac tccggaagag aaagccacca gccgcgaata cgcgaaaaaa
    70801 ggagcacgct cctgatgcag gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt
    70861 ttattatgcg tcctgtcgcc accacgctgc tgatggtggc gatcttactc gccgggatta
    70921 tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg accattcagg
    70981 tggtcacgct ctacccaggt gccagcccgg atgtcatgac ctctgccgtt accgcgccgc
    71041 tcgaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg
    71101 gtgcgtcagt tatcactttg cagttccagc taacattacc gctcgatgtc gccgagcagg
    71161 aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg cctaacccgc
    71221 cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa
    71281 ccgccatgcc gatgacgcaa gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct
    71341 cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg
    71401 tcaaacttaa cgctcaggcg attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg
    71461 ccattaccgg cgctaacgtt aactcggcaa aaggtagcct cgacggccct tcccgtgcgg
    71521 tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg
    71581 cctaccagaa cggcgcgcca attcgtctgg gcgatgtcgc aactgtagag caaggtgcag
    71641 aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc cattgtgatg aatgttcagc
    71701 gccagcccgg tgctaacatt atctccaccg ccgacagcat tcggcagatg ctgccacagc
    71761 tcactgagag tctgccgaaa tcggtgaagg tgacagtgct ttccgatcgc accaccaata
    71821 tccgcgcatc cgtcgatgat actcagtttg aattgatgat ggctatcgcg ctggtagtca
    71881 tgattatcta cctgtttttg cgcaatattc cggcgaccat cattcccggt gttgctgtac
    71941 cgctgtcgtt aatcggcact ttcgcggtta tggtgtttct cgatttttca atcaataacc
    72001 tgacactgat ggcgttaact atcgccaccg gattcgtggt cgatgacgcc atcgtggtga
    72061 tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg
    72121 gcgcaggtga aatcggcttt accattatct cgctgatctt ctcactgatt gcggtgttga
    72181 tcccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc
    72241 tggcggtagc gattttgatc tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg
    72301 cgcggatgct cagccaggag tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa
    72361 aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc
    72421 cgtggctgac cttaagcgtg gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt
    72481 tcattccgaa aggtttcttc ccggtacagg acaatggcat tattcagggc actttgcagg
    72541 caccgcaatc cagctccttt gccaatatgg cccagcgaca acgccaggtc gcggacgtga
    72601 ttttgcagga tccggcagtg caaagcctga cctcatttgt tggcgttgat ggcactaacc
    72661 cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc gttggatgaa cgtgatgatc
    72721 gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtaga taaagtgccg ggcgtcgatc
    72781 tcttcctgca accaacgcag gatctgacta ttgatactca ggtcagccgc acccagtacc
    72841 agtttacctt gcaggccacc tcactggatg cgctcagtac ctgggtgcca cagttgatgg
    72901 aaaaactcca gcaactgcca cagctttctg atgtctccag cgactggcag gacaaagggc
    72961 tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc agcatggcgg
    73021 atgtcgataa tgccctgtac agtgcgtttg gtcagcgact gatttccact atttatactc
    73081 aggctaacca gtatcgcgta gtgctggagc acaacaccga aattaccccg ggcctcgcgg
    73141 cgctggatac cattcgcctg accagtagcg acggcggcgt ggtgccgtta agctcaattg
    73201 ccaaagttga acagcgtttt gcgccgcttt ccatcaacca tctggatcag ttcccggtaa
    73261 cgaccatctc ctttaacgtg ccggataact attcgctggg cgatgcggtg caggcgatta
    73321 tggacaccga aaagacgctg aatctgccgg tggatatcac cacgcaattc cagggcagca
    73381 ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg
    73441 cgatgtatat cgtgctcggc attctgtacg agagctttat tcacccgatc accattctct
    73501 cgacgctacc caccgcaggg gtcggcgcgc tgctggcatt aatgattgcc ggtagcgaac
    73561 tggatgtgat tgcgattatc ggcattattt tgctgatcgg tattgtgaag aagaacgcca
    73621 tcatgatgat cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg
    73681 caatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc ctggcggctc
    73741 tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt
    73801 taggtatcgg catggtcggc ggtctgattg tcagccaggt gctgacgctg ttcaccacgc
    73861 cggtgattta tctgctgttc gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc
    73921 atgaagagga agcgtaagtg aagttttttg ccctcttcat ttaccgtccg gtggcgacga
    73981 ttttactgtc ggttgccatt accctgtgcg gcatactggg cttccgtatg ctgccggtcg
    74041 ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg cccggtgcgt
    74101 caccagaaac aatggcgtct tccgttgcca cgccgctgga gcgctcactt gggcgcattg
    74161 ccggagtcag tgaaatgacc tccagcagtt cgctcggcag cacgcgtatt attttgcagt
    74221 ttgattttga ccgggatatc aacggcgcgg cgcgcgatgt gcaggcggcg atcaacgctg
    74281 cacaaagttt gctgcccagc gggatgccca gccgtccgac ctatcgcaaa gctaacccgt
    74341 cggatgcgcc aattatgatc ctcacgctga catccgatac ttattcgcag ggtaaactgt
    74401 acgatttcgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt gttggtgatg
    74461 tcgatgtcgg cggcagctca ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt
    74521 ttaatcaggg cgtctcgctg gacgacgtac gcactgccat cagcaatgcc aacgtgcgta
    74581 aaccgcaggg ggcgctggaa gatggcactc accgctggca gatccagacc aatgatgagc
    74641 taaaaaccgc cgccgaatat cagccgctga ttattcatta caacaacggc ggcgcggttc
    74701 gtctgggcga tgtggcgaca gtgaccgact ctgtacagga tgtacgtaac gccgggatga
    74761 ccaatgccaa accggctatt ttgctgatga tccgcaagct gccggaagcc aatattatcc
    74821 agacggttga cagcatccgg gcaaaattac cggagttgca ggaaaccatt ccggcggcga
    74881 ttgatctgca aattgctcag gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg
    74941 agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtgttttta ttcctgcgct
    75001 cgggtcgcgc aactattatt cccgccgttg cggtgccggt ttcgctgatt ggtacgtttg
    75061 cggcgatgta cctgtgcggt ttcagtctca ataatctttc gttaatggcg ctcaccatcg
    75121 ctactggttt cgtggtggat gacgccatcg tggtgctgga aaacattgcc cgtcatctgg
    75181 aagcgggaat gaaaccgttg caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg
    75241 tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc gctgctgttg atgggcggat
    75301 tgccgggccg actgttacgc gaatttgccg tgacgctttc tgtcgccatt ggtatatcgt
    75361 tgctggtttc tctgacatta acgccaatga tgtgtggctg gatgctgaaa gccagcaagc
    75421 cgcgcgagca aaagcgactg cgtggttttg gtcgcatgtt ggtagccctg caacaaggct
    75481 acggcaagtc actgaaatgg gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg
    75541 gcaccattgc gctgaatatc tggctgtata tctcgatccc gaaaaccttc ttcccggagc
    75601 aggacactgg cgtgttgatg ggcgggattc aggcggatca gagtatttcg tttcaggcga
    75661 tgcgcggtaa gttgcaggat ttcatgaaaa ttatccgtga cgatccggca gtggataatg
    75721 tcaccggctt tacaggcggt tcgcgagtga acagcgggat gatgtttatc accctcaagc
    75781 cacgcgacga acgcagcgaa acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg
    75841 cgaaagaacc gggggcgaat ctgttcctga tggcggtaca ggatattcgc gttggtgggc
    75901 gtcagtcgaa cgccagctac cagtacacgt tgttatccga cgacctggcg gcactgcgag
    75961 aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact
    76021 ccgatcagca ggataacggc gcggagatga atctggttta cgaccgcgac accatggcac
    76081 ggctgggaat cgacgtacaa gccgccaaca gtctgttaaa taacgccttc ggtcagcggc
    76141 aaatctcgac catttaccag ccgatgaacc agtataaagt ggtgatggaa gtggagccgc
    76201 gctataccca ggacatcagt gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag
    76261 cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa tgccccacta tcggtgaatc
    76321 atcagggatt atcggcggcc tcgaccattt cgtttaacct gccgaccgga aaatcgctct
    76381 cggacgccag tgcggcgatc gatcgcgcaa tgacccagct tggggtgcct tcgacggtgc
    76441 gcggcagttt tgccggcacg gcgcaggtgt tccaggagac gatgaactcg caggtgatcc
    76501 tgattatcgc cgccatcgcc acggtgtata tcgtgctggg tatcctttac gagagttacg
    76561 tacatccgct gacgattctc tccaccctgc cctcggcggg cgttggagcg ctgttggcgc
    76621 tggagctgtt caatgccccg ttcagcctaa tcgccctgat agggatcatg ctattaatcg
    76681 gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc caacggcacg
    76741 gtaacctgac gccgcaggaa gctattttcc aggcctgtct gctgcgtttt cgcccgatta
    76801 tgatgaccac cctggcggcg ctgtttggtg cgctgccgct ggtattgtcg ggcggcgacg
    76861 gctcggagct gcggcaaccc ctggggatca ccattgtcgg cggactggta atgagccagc
    76921 tccttacgct gtataccacg ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt
    76981 tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac agatcttccc gacagcaccc
    77041 gttggcaatt gtggattgtg gctttcggct tctttatgca gtcgctggac accaccatcg
    77101 taaacaccgc ccttccctca atggcgcaaa gcctcgggga aagtccgttg catatgcaca
    77161 tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg
    77221 cggacaaagt cggcgtgcgc aatattttct ttaccgccat cgtgctgttt actctcggtt
    77281 cactgttttg cgcgctttcc ggcacgctga acgaactgtt gctggcacgc gcgttacagg
    77341 gcgttggcgg cgcgatgatg gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc
    77401 gcgagcaata tatggctgcg atgacctttg tcacgttacc cggtcaggtc ggtccgctgc
    77461 tcggtccggc gctcggcggt ctgctggtgg agtacgcgtc gtggcactgg atctttttga
    77521 tcaacattcc ggtggggatt atcggtgcga tcgccacatt gctgttaatg ccgaactaca
    77581 ccatgcagac gtggcgcttt gatctctccg gatttttatt gctggcggtt ggcatggcgg
    77641 tattaacccc tggcgctgga cggcagtaaa ggtacaggtt tatcgccgct ggcgattgct
    77701 ggcctggtcg cagttggcgt ggtggcactg gtgctttatc tgctgcacgc cagaaataac
    77761 aaccgtgccc tgttcagtct gaaactgttc cgtactcgta ccttttcgct gggcctggcg
    77821 gggagctttg ccggacgtat tggcagtggc atgttgccct ttatgacacc ggttttcctg
    77881 caaattggcc tcggtttctc gccgtttcat gccggactga tgatgatccc gatggtgctt
    77941 ggcagcatgg gaatgaagcg aattgtggta caggtggtga atcgctttgg ttatcgtcgg
    78001 gtactggtag cgaccacgct gggtctgtcg ctggtcaccc tgttgtttat gactaccgcc
    78061 ctgctgggct ggtactacgt tttgccgttc gtcctgtttt tacaagggat ggtcaactcg
    78121 acgcgtttct cctccatgaa caccctgacg ctgaaagatc tcccggacaa tctggcgagc
    78181 agcggcaaca gcctgctgtc gatgattatg caattgtcga tgagtatcgg cgtcactatc
    78241 gccgggctgt tgctgggact ttttggttca cagcatgtca gcgtcgacag cggcaccaca
    78301 caaaccgtct ttatgtacac ctggcttagc atggcgttga tcatcgccct tccggcgttc
    78361 atctttgcca gagtgccgaa cgatacgcat caaaatgtag ctatttcgcg gcgaaaaagg
    78421 agcgcgcaat gaagttctgg cgacccggta ttaccggcaa actgtttctg gcgattttcg
    78481 ccacctgcat tgtcttgctg atcagtatgc actgggcggt gcgtatcagt tttgagcgtg
    78541 gctttattga ttacatcaag catggtaatg aacagcgatt acaactgtta agtgatgcgc
    78601 ttggcgagca gtatgcgcag catggcaact ggcgcttcct gcgcaacaat gatcgctttg
    78661 tctttcagat cctgcgttca tttgaacacg ataattcgga agataaaccc ggcccgggta
    78721 tgccaccgca cggctggcgt acccagttct gggtggttga tcaaaacaac aaagtgctgg
    78781 ttggtccgcg agcgccgatt ccacctgacg gtacacggcg acccattctg gtcaacggtg
    78841 cggaagttgg cgcggtgatc gcctcccccg ttgagcggtt aacgcgcaat actgatatca
    78901 atttcgataa acaacagcgg caaaccagct ggttgattgt cgccctggca acgttactcg
    78961 cggcacttgc cacttttctg ctggcgcgcg gtttactggc accggtaaaa cgacttgtcg
    79021 atggcacgca caaactggcg gcgggcgatt tcactacccg cgtaacgccc accagtgaag
    79081 atgaactggg caaactggcg caagacttca accagcttgc cagcacactg gagaaaaacc
    79141 agcaaatgcg gcgcgatttt atggccgata tttctcacga actgcgtacg ccattagcgg
    79201 tgctgcgcgg tgaactggaa gccattcagg atggcgtgcg taaattcacg ccggagacgg
    79261 tggcgtcttt acaggcggag gtcggtacac tgaccaaact ggttgacgat ctccatcagt
    79321 tgtcgatgtc tgatgaaggc gctctcgcct atcaaaaagc accggtagat ttgatcccac
    79381 tgctggaagt ggcgggcggc gcatttcgcg aacgattcgc cagtcgtggc ctgaaactgc
    79441 aattttccct gccagacagt attaccgtat ttggcgatcg cgaccgttta atgcagttat
    79501 tcaataattt acttgaaaac agcctgcgct atactgacag cggcggcagc ctgaaaatct
    79561 ctgccgagca gcacgacaaa acggtgcgcc tgacctttgc cgacagtgcg ccaggtgtca
    79621 gtgacgagca gctacaaaaa ttatttgaac gtttttatcg caccgaaggt tcccgcaacc
    79681 gcgccagcgg tggttccggg ctggggctgg cgatttgcct gaacattgtt gaagcacata
    79741 atggccgcat tattgctgcc cattcgcctt ttggcggggt aagcattaca gtagagttac
    79801 cgctggaacg ggatttacag agagaagtat gaccgagtta ccaatcgacg aaaacacacc
    79861 acgtattttg atcgtggaag atgaaccgaa gctggggcag ttgctcattg attatctgcg
    79921 tgctgcgagc tatgcgccga cgcttatcag ccacggcgat caggtactgc cgtatgtgcg
    79981 ccagacacca ccggatctga tcctgttgga tctgatgctc cctggcaccg atggcttgac
    80041 gctgtgccgg gaaattcgtc gtttttctga cattcctatc gtgatggtga cggcaaaaat
    80101 cgaagagatc gaccgcctgc tcggactgga gattggcgca gatgactaca tctgcaagcc
    80161 gtacagccca cgggaagtgg tagcgcgcgt caaaaccatt ttgcgccgtt gcaaaccgca
    80221 gcgcgaattg cagcaacagg atgccgaaag cccgttgatt atcgacgaag gtcgttttca
    80281 ggcttcatgg cgcggtaaaa tgcttgacct gacacctgcg gaatttcgtc tgttgaaaac
    80341 gctctctcat gaaccaggaa aagtgttctc ccgcgagcaa ttgctcaatc atctttatga
    80401 cgactaccgc gtagtaaccg accgcaccat tgacagccac attaaaaacc tgcgccgcaa
    80461 gctggaatct ctcgacgccg aacagtcatt tatccgcgcc gtttacggcg ttggttaccg
    80521 ctgggaagcc gacgcctgcc gcatcgttta gttttagcga catgactttg ttagccggag
    80581 atggcgttca gaccagatcc ggcaacatta tcccacgcat ggtcagcaaa ctgacattat
    80641 ctctccgccc tggcaggcat cagaaaatcg ctcatacttt aatcggtaaa cagcaccttt
    80701 agatgctgtt ttcgatacgc aatttcaatc aaggagtcat tatggctggt tggtttgaac
    80761 tcagtaagag cagtgataat cagttccggt ttgtgctaaa agcgggcaat ggtgagacta
    80821 tcctcaccag cgagctgtat acatcaaaag cctctgcgga aaagggcatc gcgtcagtgc
    80881 gtagcaacag cccgcaagaa gaacgctatg agaaaaaaac ggcaagtaac ggcaaattct
    80941 atttcaatct gaaagccgcc aatcatcaaa ttatcggctc cagccagatg tacgccaccg
    81001 cgcaatctcg tgaaaccgga attgcctccg ttaaagccaa tggcacaagc cagacggtga
    81061 aagacaatac gtaattacga tgccgggcgt gggttgttgg cgtccggctt tattttctct
    81121 catcacagag ttactatccc tcaagaaatt attaaccatg aaatgagttt tctcctcata
    81181 actgataagg aaaattatgc tcaaaggatt gcgtacatta ttttcacgca gggttttgtt
    81241 taacgtcgaa atgaataatt ctgttgactg tgaattactt ctcatccagc aacaactcca
    81301 ggcattgata gccgaaccag gcaattattt agcacatgat tgtgatctca acgatactct
    81361 ggttctttgc cagcttaccc atcatcacaa tggatatata cgagaaaagg ccgtgcagta
    81421 tctggggcac aaagaggatg ttgcggctat agaagaatta ttacttgcag ctaatgactg
    81481 ggtaaaagag gttcgccttg ctgcaaccat agcattacgt catttaatga aggataagaa
    81541 tgcagcggca tttgccgtta atctacccat gatacgtaac cttctccaat gccagcgtta
    81601 cgatcaccgt gcatttgtag acgagatact tagatattta ttgacagaaa ataaccgccc
    81661 acaattaacg tattggctta cttgccctga taaaaagctc tcccgcgcgg tattacaaac
    81721 gctgattgaa tacggttact ttaatgatga atcttcactg ctttcaatac tcgataaacc
    81781 cgatgagggt ttgcgaatgc tggcgaccac ccactggctg cgtcaacaat taccattaag
    81841 cgaagcggta ttaactcgtc tgttaaaaga ccgctggccg cgtattcgac aagcaacgtt
    81901 atttagcctc actgaccgag caatagaaat gcctccggcg ttgcacagca cattactgct
    81961 ggataacaat atgttgatcc gcctacgcgc taaaaatatg cttcacgaag tcatggatgt
    82021 tccgcaattc tggcgtcatg ttgtcacttc tgcggaatat actccctcac agcgacgggc
    82081 cgctttatac gggctggatt ccattcatga tcccaatatt ttaaaactgg cagaatgggg
    82141 attatcacag aatgtattcc cactgcgttt agccgctatg cacattcttg caaaagctaa
    82201 tcctcgttgt ggcgtcaaag agactattct gaccacgctg gctaacccgg acgccgccgg
    82261 gttgcgattt atggttaaca tctgtgtatg gtgtcgcgtt ccgctcacgt tcgaggaaat
    82321 acgtcagttg caggaaaacg cgccgtcagt taaacatgct tgcgcatatt gtcggctgta
    82381 tcataacttg aacaaatggg acggtttaat tcttcttctt caatcgcaac acaagctaac
    82441 tgaagaattt gcaggtaaac agctcgcgat atggcaacgc aatttcaatc tttcgggcat
    82501 tcaaccgaat gctcttcagc gtcagcaact tcaggcgctt tttacccgca atccggaact
    82561 tcacaacaga ttatggggtt acattccttt taaataactt cgaattatag acttacgatg
    82621 acaaatgtac ctgctgacga gcggaaccaa aacgaaacaa tacgtgaagc tttacgcttg
    82681 ctacgagaaa aacttgccga gggactacgc agtggcgaag cccaaccgtg ggaaaaggat
    82741 ctgtttttac ataaagtgaa ggccggtaac aaataaatcc cccagtcttt tccctcacat
    82801 ttgcactgga gccaaccccg ccgattgcgc tacaatgccc gcccttaaag tgggggcact
    82861 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    82921 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    82981 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac
    83041 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    83101 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    83161 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    83221 atgctggtgc gtgagtactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    83281 gtaaactggg cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct
    83341 cgcgaactgt cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatc
    83401 gagatcttcg ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc
    83461 tatatcaaca agcgcgatcc gaaccagggt acctgcacca acgcctgccg ctgggagtac
    83521 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatc
    83581 ccggtgcaaa atgttgagcc gacgctaggt atcggcgcgc caaccgacaa agtgtttatg
    83641 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    83701 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    83761 ggcgtgcatt cgctgaaaat cgaaggccgt actaaatctt tctactattg cgcacgcacc
    83821 gcacaggttt atcgtaaagc tatcgatgat gccgctgcgg gcaaaccgtt cgataccagc
    83881 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    83941 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    84001 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    84061 aaattctccg ttggcgacag ccttgagctg atgacgccgc aaggcaacat taactttacc
    84121 cttgagcaca tggaaaacgc caaaggtgta gcgatgccgg tcgcaccagg cgatggttat
    84181 actgtgtggc tcccggtccc gcaggatctt gaactaaatt acgcgctgct gatgcgtaat
    84241 ttctccgggg aaaccacgcg taacccacac ggtaagtgat taatttcgat tatttttccc
    84301 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    84361 ttcgctgaaa aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    84421 tgtaatctcc cttacacggg cttatttttt atgcataagc cctatccctg gtcaccgtct
    84481 tccattgacc acatcgatag aatctccctt catagcacga tgcctttcac gtaacggcat
    84541 cgtgctcgca caggttccgg ctaagcacaa ccagaacgcg catgtttgat gcttaccaaa
    84601 aaatattctc actctccaca tttgaatgtc agacgagcga caccatgtaa tcctacacct
    84661 tctgtcttca gctcaactat ttgcattttt ttgccctgag taacacagaa atgagttgca
    84721 tcatttttta ctatattttc tgcaccagat attctactcc tggctaaaga agcttcggct
    84781 tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat
    84841 ttaccgatac ttgtctcatc attatgcaca gcacaaccag acataataaa aatactaatt
    84901 aatgatatag cagctatctt tttcatctca cctttcccca ttaaatacca acgacactct
    84961 ctagtgttta aatataataa tagctgatca ttatatttga ataggattat aataaatgtt
    85021 ctgtacaaca tttcctacat aagtaggact tacagacatg aaggcccttt ccaggtaact
    85081 ccatggggct ttaatatatt tggaatagta tatttaagat gccactgttt ggttgaatat
    85141 taggcatatg ctcttttttg aaatttatcg gtggctaccg ttaatattcg ctgttcccat
    85201 tgcaagctcc tggtggtaac cactgaatcc tccatacttg aactgacttt ttatcctccg
    85261 actttcatcc tgttctgact ccaccttttc ttttctgctc tacactatct acagaccaat
    85321 cataaaggca catacgatca tggcagaatt tcccgccagc ttactgattc ttaatggcaa
    85381 aagtactgac aatctaccct tgcgcgaagc aattatgctg ttgcgtgagg aaggaatgac
    85441 gatccatgtg cgggtcacct gggagaaagg tgatgccgca cgatttgtag aggaggcccg
    85501 gaagttgggc gtcgcaacgg taattgccgg tggtggcgat ggcaccatta atgaagtttc
    85561 tacggcgttg attcagtgtg agggggatgg catacccgcg ctgggaattt tgccattagg
    85621 aaccgccaat gattttgcca ccagtgtagg gattcctgag gcactggata aggcgctgaa
    85681 actggcaatt gccggtaacg ccattgcgat agatatggcg caggtcaaca aacaaacctg
    85741 ttttattaat atggcgacag gcggatttgg gacgcgtatt accacagaaa cgccggaaaa
    85801 attaaaagcc gcgctgggtg gcgtctctta catcattcat ggcttaatgc gcatggatac
    85861 tctgcaaccg gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag gtgacgccct
    85921 ggtattggta ttggtaacgg gcgtcaggcc ggtggcggtc agcaattgtg tccgaacgcg
    85981 ttaattaacg atggcttgct gcaactgcgc atttttaccg gcgatgaaat tcttccggct
    86041 ctcgtatcaa ccttaaaatc tgacgaagat aacccgagta ttatcgaagg cgcttcgtcg
    86101 tggtttgata tacaagcccc acacgaaatc acttttaatc ttgatggcga accgttgagc
    86161 ggacaaaact ttcatattga aatacttccg gcggcgttgc gttgtcgatt accaccagat
    86221 tgtccattat tgcgttaatc aaactattct ctcttgaacc agggagaatc cgaaatgccg
    86281 gatgcggtgt aaatacctta tccggcatac agaacaatac tacaccgtaa ttaattttac
    86341 ccgtgtcttc tgcaatgcca gtttatccac ctctgataca gcatcatcgg taataataat
    86401 gtcaattttt tcgattggca gtacctgatt aaaaccgcga cggttaaatt tcgatgaatc
    86461 gagaacggca atcactttat tagcggctgt gaccatcgca ccgctaacgg aataaccctc
    86521 attaaaggtc gtaataccat taaccgcatc aataccatca gcaccgacaa acattaaatc
    86581 ggcattaata tcctgcaggg aacgttcagc aatagatcca tgcatagatc gcgttttatg
    86641 gcggaccgtg ccaccgcaga cgaccagagt aatgtcttta ttttcggaaa gggcaaacgc
    86701 tgccgggaga ctgttggtaa tcaccgtgat atttttagcg gtcattaatc cttcggcgat
    86761 aagcattgtg gtacttccgc tgtcgagaat aacggtcatc ccttcatgga tcattttgac
    86821 tgccgcctgc gctattctgt tttttggcgc gctggcgagc tgaaatcgct ctttaaaccc
    86881 gacttcctgg gtctcggttt cactattacc ggacattatt ttcgccgcac cgccatgaaa
    86941 gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg gcacggattg tcgcttccga
    87001 ggcagcaaat actcccgcca gatcctgaac aagcacggtt ccctgttcat tcactaattg
    87061 gatgatctta ttccttcgct cgaatgaatt catcgtcatt tacccatatt caattgtggc
    87121 tagtgtaaac gaagggggac cattggtgaa tgatctgttt tgcacaatac cgggccagca
    87181 tcacgctggc ccgcggtttc atggaatgag caacactttg cccggcatgg cattacgagc
    87241 gatgtcacgc accgcctggg tgaagctttc aaagcttcca cggtgagcga ttaatggctc
    87301 caggcttaac ttacgttctg tcagcaaccg gctcgccgtt tcccactcct gccccggcca
    87361 agggctggaa tagttcatcc aactgccgat aaccgtcagc tctttacgca atattttgcc
    87421 aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg cccaccagcg ccagttgggc
    87481 atgaggaccg gcaatctcta ccgccagttc gacagtttgc ggtacgccag ccgtctcgag
    87541 gataagctga ttaaaacgca gttcgcgtaa aacgctctgc atttgcggcg cgctcatttc
    87601 gctactgtta aatgtttgca tcgaaccgaa agattttgcc agtgccagtt tttctgagct
    87661 aatgtcgata gccgtcacac tctttgctcc cagcgcgaca gcgcactgaa tagccagcag
    87721 gccaatggtt ccggcaccaa taataataac gtttttattc tcacaacctt gcgctaaatg
    87781 aaaagcatgc agaccaacgg taatcggctc aataaaagcc ccatcctcaa taggcatatc
    87841 cgtgggtaga gcaaagacat tttttcgctt aacgacaata tattcagcaa atccaccatc
    87901 acgccgcgag ccaataaaat catattttgc gcactgggaa taaaaccctt tcagacactc
    87961 tggacaagta aaacagggta ataacggcac acaggcaacc gcatcgccag ggtgtaaatc
    88021 atcaacaccg gatcccaccg catcaatata gccgctaaat tcatggccta acgttattgg
    88081 ataataatgt gcaccatttt taaatatcct gggtaaatcg gaaccacata agcccgagct
    88141 ggcaattttt acccgcacct catcctgatg tttaatttca ggaatgacgc tttctgcaac
    88201 gcgcacgata ccatcagtat cattcaccac tgatttcata aaaactcctg attgttaagg
    88261 gggataaact ccccctcagt taaaaattat tctgcgagaa cggctttctc ttgtttaata
    88321 aaaccacgcg ctctacgcca ggtaatgaaa ataccggtca gataaattgc gccgataata
    88381 atgaaaccgg gaatattttg cggggagaaa acctgaatca gtaaccaggt aatgggagaa
    88441 ccgccctgat ccattgaagc caccataccc ccggctttta acgccccagc attagccgcc
    88501 agttgggtgt gcaaaccaat agtttgcgtc gcgatccaca gagtgatgct catgatgatg
    88561 acacccgaga ttaaggtgcg gaacagattt ccacgatgca cggcgactgc catcgccaca
    88621 aagaagccga tggtggcaag atcgccaaac ggcagtacct gattacccgg aacacaaaca
    88681 gcaattaaaa tggtgagcgg gataaaaatc aggcttaccg ataccaccgc cgtatgcccc
    88741 agcagtaatg cgggatccag gccaatcagg aactcctgac cgccgaactt cgcctgtaaa
    88801 cgactacggg cctgcttagc gatgggcgtt aaaccatcca tgatgggttt aatcacccgt
    88861 ggcattagca gcatcactgc cgccgttttt accgccagct gcaatacacc tttgacatcg
    88921 taacccgcga ggatgccgat aatcagcccc atcacaaaac ccacggtgac aggttcgcca
    88981 aaagggccaa atttgcgctg aatatcatcg gcgctaaatt taattcggtt aacgcctggg
    89041 attttctcga tgatagcatc gaccagcacc gcaatcggcc ccatatacgc cgacgtaccg
    89101 tgcggaatag caataccttc cagctcaaag aaatttcggg tatcgcgggc aaaccagtcg
    89161 ccgagcttat aaacaaacgc cgcgtgaatt accacacccg ccatccctat catccatgaa
    89221 ccggttgcca gatgcagcaa cgcgccggtg aaagtcatat gccagatatt ccagatatca
    89281 acatttacca cccgcgtcat acgggtcagt aacatcgcca cgttaaccag aatagcaatc
    89341 ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg gtgaagagcc cggccagccg
    89401 acatcgacca catgcagatt caggtcgaaa ttttccgcca tcgctttcgc cgccggacca
    89461 atggaatcca gcattaagcc aatcacaagg ccaatgccaa caaagccaat ccctatatga
    89521 agacccgctt taaagcaatc gcctgccttc atgcctaata ttttagaaaa aataatgatg
    89581 acaatcggca gcatcaccgt agggccgagg tcgagaatat aacgcatgac ttctgaaaac
    89641 ataggtcacc cctgtaagat agtcagaatt ttattttgta atgcttcgat accgacacca
    89701 gaaacaaaag gcatgccgtg aactaacgga atatcgccaa aactacgatc caccctggca
    89761 gtagtgcata tcaaatgcac gccatccata taggtttcta tttcattaac ccgacactgg
    89821 attaattcaa caggaatatt atgattctga cacaattctt taatttcttc cgccgccatc
    89881 gtagaggtcg caaccgcgcc tccgcaagcg acaataatct tgcgtttcat aaatagtcct
    89941 ttttattgtt acagagggat ttattcgtct gaatctaaaa catactttgt gaagtattcc
    90001 tttaactggg tttcaggaag agtgattagt gtctcgacga tatcgggctg ttgtaacttg
    90061 ccaaataaac agcgtaaaag tttcaattgc tgctgcggat tttccacaat caacgcaata
    90121 accaacgata cctccacgtc gttatcatca tccgcttgct gaaaatgaac tttatttgtt
    90181 ggccttaaca gataaatggc tgacgactta gcatgaatcg cctcacaatg cggtattgca
    90241 atggcgtgct gctcaagcat tatcccggta gggaattctg cttctctggc aattaatgcc
    90301 tgtggccagg tatcatgaac cacacctttg gcgagcatct cattaccgat atgggttaaa
    90361 acttcgctac gatcgacaaa agaaattccg ctacgaacaa acaggttagt catataccgt
    90421 ccttattccg cacagccgta gcgataggcg cgcaaaacat cataaatttt atccataatc
    90481 agctaatgcg gtattgctga taattccccg gactgaatgc gttcaaattg tttgggaaga
    90541 tactgactaa tcatgcccag tgggatgtcc acgccttcaa gattcaccat catcgtttcg
    90601 acgctatttt taatccgact atgcggccag taataacgaa tacgatccga caggctgtaa
    90661 cgaatatcca gtaatgaatc gttaaaaccc gtacgataat attttttcca gtattgcggt
    90721 tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc tgcgattttc aggggcgata
    90781 agttcctgct caatttgtgc cagtgcaaat atcgcctcgc gtaaagcaaa ggttaatgcg
    90841 ggaccgactt tcaatattgc aaagtgatcg cggactaatt cccaataagc cgtccgggtc
    90901 tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt tttctatcca ttgcgccagc
    90961 ggctgcgctt cctgcggctg ataatggata atattgctgt gatcaaattc cacgcccggc
    91021 tgcaccacga tggcaatcac gcgtgttaac gcctctgtca gcccacggac aataaaggcc
    91081 ttttgatgcg tacgtaaagt attggcggca tcttcaacat gggtgatgtg tactgactga
    91141 atggcgctgg cctcaccgcc cggaaccgga acttcggtgc caatgacata gctcagttgc
    91201 tcacgctggc aatctgtcgc cacactttcc gcagcaaagc aaagcacagc agctcgttcc
    91261 gcaaccgttt ctggtgctaa cggtatggga tcccccgcgc aggacattga cgcatcaaga
    91321 tgaattttac tgaagccggc acgaacatat tcctttacca gctcgacgga tttttccatc
    91381 gccgcatccg cattttcttg ctgccagcag tttggcccca gatgatcgcc gccgagaata
    91441 atgcgttcgc gtgcaaaccc aactttatcg gcaatcgtaa aaacaaattc gcgaaagtct
    91501 gccggtgtca ttccggtata accgccaaat tgattgacct ggtttgacgt tgcttcaatc
    91561 agcactttgc gcgtgctgtt gcgatcaaat gccagcgccg cttcgataac caacggatgg
    91621 gcagaacaga ctgaacatat gccgatatgt tcaccagctt tatgccgggc aattaacgtt
    91681 ttcatgtttt ttccttatta aatggcaggt gcgttatgcc ctgccctcgc agccacaatc
    91741 ggcaatcact ttgctcacca catcgcgcat tgcggattta gccgactgca aataatcccg
    91801 gggctcggtc gcttcagggt gctcggtcag gtaatttttt aacgcctgcg agaaggcatt
    91861 tttcagctcc gttgcaacgt tgattttgca tatccccagt ttgatggttt gctgaatatc
    91921 cttagtcgat aaccctgacg cgccatgcag caccagcggt aagttcaccc actggcgaat
    91981 gttctccagt ctagaaaaat caagcgccgg tgcgctggca tacatcccat gagccgtgcc
    92041 gatggcgacc gccagggaat caattccggt tgcctcggca aattcacgcg cctgagcggg
    92101 gttggtataa aaagcatcgg cttcattgac ttgcacatca tcttcctggc cgccaagttg
    92161 ccccagctcc gcttcgacgc tgacatcaaa gcgatggcaa aaatccacca cctctttgac
    92221 ccgtgaaata ttttgcgcaa aaggcaaatg cgaggcgtca atcatgactg agcgcacgcc
    92281 agaacgaacc ttctgagcga tatcgtcaaa tttcgtgtga tggtcgagat gaattgccag
    92341 tggatggtga tattgcttcg ccatcgcgct gaccagcgcc aacagatttt ctgtaccagc
    92401 atgagtaaat gtgccaggcg ttccggcgat gatgaccggc gcatgcaggt tggcagcggt
    92461 ttccaccacc acttgcatcg tttcgagatt gtgaatattg aatgccggaa ccgcataacc
    92521 gccgcgctgt gcgttgttca gcatctgctt tgtcgatacc acgtgcattt tcatatcctg
    92581 tcgtttgttt tcgttttcaa aatataatga aattatttgt ttttaaatat cgagataacg
    92641 atcacaaaaa cgacaatatg aaaattattc gaggagtgaa aggcaaaaaa acggcctccc
    92701 gatagggaag ccgtagcaaa gtgcgcgtgt ttttatgccg gatgcggtgt aaacatacgt
    92761 ggcagtgcta tgtaggcatg ataagacgct tccgcgtcgc atcaggcata ggttgccgga
    92821 tgcagtgtaa acgccttatc cgtcctacgg ggtggtgttg ttcgtataag gcgtatcagg
    92881 caatagtaat tttgctatcg agataaacgt cctgcacggc gttaatcagt ttcacgccgt
    92941 cagccatcga tttcttgaac gccttacgtc caagaatcag ccccattccg ccagcgcgtt
    93001 tgttgataac cgcagtacgc actgcatcac tgaggtcagt ttcaccgcct gcagcaccgc
    93061 cggagtttat caacccagcc cggcccatat agcagttagc taactgataa cgcaccagat
    93121 caatcgggtt ttcgctggtt aacttgctgt acacgcgatc gtcggtataa ccgtaattaa
    93181 tcgctttata gccgccgtta ttttccgcca ttttttgttt gacgatatct gcgccgatgg
    93241 ttgccgccag atggtttgcc tgaccggtca ggtcggcgga aacatggtaa tcaacgccat
    93301 ctttcttaaa ggcggagtta cgcaaatagg cccacagcac ggtcaccatg cccagctcat
    93361 gcgcacgttc aaaagccgca gaaatttctt caatctggcg acgtgactct tccgagccaa
    93421 aatagatagt cgcaccaacc gccaccgcgc ccatgttgaa agcctgctcc acgctggcat
    93481 acagcgtttg atcgtaggta ttcgggtaac ttagcgtctc gttgtgatta agtttgacga
    93541 gaaatggaat gcgatgcgca tagcgccgcg ataccgacgc caacacgccg taagttgata
    93601 ccacacagtt acagcccgct tcgatcgcca gttcaacaat gtttttcggg tcaaagtaga
    93661 gcgggttagc agcaaatgaa gctccggcag agtgctcaac gccctggtca accggcagaa
    93721 tagaaagata tcctgtgcca gccagacgcc cggtgttgta caacgtctgc atattacgta
    93781 acaccgctgg cgggcgatta ttgtcaatca ttacgcggtc tacgtagtca tgtccgggga
    93841 gataaagctg gtcagaagga attgtcatac aacggtgctg taaaaggttg tcggcgtctt
    93901 tgccaagcaa ctgcgcaata tctgtcatta ctatgctccc gtaaattccg attggatatc
    93961 ggctatggat tgtcctggcc cgccttttgc gggcaatcat aatcctggtc gttacgggcg
    94021 agtttttcca tcttttggat gttttttcag cgttttctgt tggctcgatt catcagaaaa
    94081 aatgttagcg cggtcaagtt ttcaccgtaa agatatttaa aaggtattat taagtggtat
    94141 tgtcatcgcg taccttacat tacctgtcat gaaggaatta aaagatgaaa acaacagcaa
    94201 agctgtcgtt catgatgttt gttgaatggt ttatctgggg cgcgtggttt gtgccattgt
    94261 ggttgtggtt aagtaaaagc ggttttagtg ccggagaaat tggctggtcg tatgcctgta
    94321 ccgccattgc ggcgatcctg tcgccaattc tggttggctc catcactgac cgctttttct
    94381 cggcgcaaaa agtgctggcg gtattgatgt tcgcaggcgc gctgctgatg tatttcgctg
    94441 cgcaacagac cacttttgcc gggttctttc cgttactgct ggcttactct ctaacctata
    94501 tgccgaccat tgcgctgact aacagcatcg cttttgccaa cgtgccggat gtggagcgtg
    94561 atttcccgcg cattcgtgtg atgagcacta tcggctggat tgcctccggt ctggcatgtg
    94621 gtttcttgcc gcaaatactg gggtatgccg atatctcacc gactaacatc ccgctgctga
    94681 ttaccgccgg aagttctgct ctgctcggtg tgtttgcgtt tttcctgccc gacacgccac
    94741 caaaaagcac cggcaaaatg gatattaaag tcatgctcgg cctggatgcg ctgatcctgc
    94801 tgcgcgataa aaacttcctc gtctttttct tctgttcatt cctgtttgcg atgccactgg
    94861 cgttctatta catctttgcc aacggttatc tgaccgaagt cggcatgaaa aatgccaccg
    94921 gctggatgac gctcggccag ttctctgaaa tattctttat gctggcatta ccgtttttca
    94981 ctaaacgctt tggtatcaaa aaggtattat tgcttggtct ggtcaccgct gcgatccgct
    95041 atggcttctt tatttacggt agtgcggatg aatatttcac ctacgcgtta ctgttcctcg
    95101 gtattttgct tcacggcgta agttacgatt tttactacgt taccgcttac atctatgtcg
    95161 ataaaaaagc ccccgtgcat atgcgtaccg ccgcgcaggg gctgatcacg ctctgctgcc
    95221 agggcttcgg cagtttgctc ggctatcgtc ttggcggtgt gatgatggaa aagatgttcg
    95281 cttatcagga accggtaaac ggactgactt tcaactggtc cgggatgtgg acttttggcg
    95341 cggtgatgat tgccattatc gccgtgctgt tcatgatttt tttccgcgaa tccgacaacg
    95401 aaattacggc tatcaaggtc gatgatcgcg atattgcgtt gacacaaggg gaagttaaat
    95461 gaaaacagaa cgtattctcg gtgctcttta tgggcaggcg ttaggggatg cgatggggat
    95521 gccctccgag ctttggccac gcagccgcgt taaagcacac tttggctgga ttgaccgttt
    95581 tcttcctgga ccaaaggaga ataacgcggc ctgttatttt aaccgcgccg aattcaccga
    95641 cgatacctcg atggcgctgt gtctggcgga tgcgttactg gaacgtgaag gcaagatcga
    95701 tccggatctg attgggcgta atattctcga ctgggcgctg cgtttcgacg cctttaacaa
    95761 aaacgtacta ggtccgacct cgaagattgc gcttaacgcc attcgcgacg gtaaacccgt
    95821 tgctgaactg gaaaacaacg gcgtgaccaa cggcgcagcg atgcgcgtct cgccattagg
    95881 ttgtttgctt ccggcgcgtg atgttgattc ctttattgat gatgtagcgc tggcgtccag
    95941 cccgacccat aaatccgatc tggcggttgc aggcgcggta gtcatcgcat gggcgatttc
    96001 tcgtgccatt gacggagaaa gctggtcagc gattgttgat tcactgcctt caattgcgcg
    96061 acatgcccag caaaaacgca ccaccacgtt cagcgcctca ctggcggcgc ggctggagat
    96121 tgcgctgaaa attgtgcgca atgccgacgg caccgaatcc gccagcgaac agctttacca
    96181 ggtcgttggc gcaggtacca gcactattga gtccgttccg tgcgccattg cgctggttga
    96241 actggcacaa accgacccga atcgctgcgc cgtcctgtgc gctaaccttg gcggcgacac
    96301 agacaccatc ggtgctatgg cgacggcaat ttgcggcgcg ttgcatggcg ttaacgctat
    96361 cgatcctgcg ttaaaggcgg aactggatgc ggtaaatcag cttgatttca accgctatgc
    96421 cacagcgctg gcgaaatatc gtcaacaacg ggaggcggta tgagcggcgc tcgattacac
    96481 acgctgctgc ctgaattaac cacgcgtcag ccggtgatgg tcgtcggcgc ggcggtcatt
    96541 gacgtgatcg ccgacgctta tgccctcccc tggcgtgggt gcgatatcga actgaaacag
    96601 cagagcgtta acgttggcgg ctgcgcactg aatattgccg tggcgttaaa gcgcctcggc
    96661 atcgaagcgg gtaatgcctt gccgctcggt cagggcgtgt gggcggagat tattcgcaac
    96721 cggatggcaa aagagggctt aatcagcctg atcgataacg ccgaaggtga taacggctgg
    96781 tgtctggcgc tggttgagcc ggatggcgaa cgcactttta tgtcattcag cggtgtggaa
    96841 aatcagtgga atcgccagtg gctggcgcga ttaaccgttg cgcctggcag cctgctctat
    96901 ttttccggtt atcaactggc ctcgccctgc ggcgaattgt tagtggaatg gctggaagag
    96961 ctgcaagacg tgacgccgtt tatcgatttt ggcccacgta ttggcgatat cccggatgca
    97021 ttactggcgc ggatcatggc ctgtcgacct ttagtgtcgc tcaatcgtca agaggctgag
    97081 attgccgccg aacgttttgc tttatccgca gagataacaa cacttggcaa gcaatggcag
    97141 gagaaatttg ccgcgccgtt gatcgttcgt ctcgataaag aaggcgcatg gtatttcagc
    97201 aacgacgctt ctggctgcat tccggcattt ccaacgcaag ttgtcgacac cattggggcg
    97261 ggcgacagtc atgccggtgg cgtacttgcc gggctggcct ctggtctgcc actggcggat
    97321 gccgtattac tgggcaatgc agtggcgtcg tgggttgtcg ggcatcgggg cggtgattgt
    97381 gcgccaacgc gcgaggaact actcctcgca cacaaaaacg tatagatcgc tgcgacagtg
    97441 gctaatgctg tactcaatag gccgttgctg ttggtcaagc gccacttgct tgatcaccag
    97501 caccggtatt ttgctatcta actgaatatg tgactgaaac tcggcatccg gcatccgggc
    97561 gctaacgcgg gaacgcgtac gttgtgggta aatgtgctgg ctgcagaaat agtcataaag
    97621 tgaaatcccg atggcatcaa catcatgaat taaatgcgcc ggaacccacg attcctcaat
    97681 cgataccgct tcttcatcga catagcgaat acgcttaagc aaaaagacat cacttcctgc
    97741 ttcgaccgcc agctgctgcg cgacttcttc gggacatttc acgacacgct tgttaaccca
    97801 tagcgtatcg ggctttttcc cgcgcaatac cacctgctga gaaaaacctc gcgcttcttt
    97861 caacgagtat tcgaagatgt tgttgatctg cgtgccgtaa ccgcgcgaac gcgtcaccac
    97921 accctcttct tccagcgcct gcatcgcctt gcgcaccgta atgcgcgaaa cgccggttaa
    97981 ctgacttaaa tcacgctcac cgggcaaaat attgccatgc tccagcaccc cgctgcgcac
    98041 ggcatttttt accgtttcgg caaacttaat ataaagcggc gtgttatccg ccgctaaaat
    98101 acgctcgttc agttgggcaa tcagctgggt atgcgcttgt tccatttgtc tttttcctgc
    98161 cggggagtct ggtgggagta tatcactgct tcttatttaa ggcgtttctt taatcccatc
    98221 aacgaatgcc tgcaactcct ccaccgtgcc attaaataca ttaaaatcca ccggaccatt
    98281 gatgccatct acctgtccac ggtcggaatg ctgccagaag cgccaggcca tgccgtcatt
    98341 gtccggacga cgttgataat agtgagccac ccaccacgga tattcattga aatagcccgc
    98401 cagattcgtg tgataaaaaa cggctcctga gtaaataatc ggctttttcc ccgtactttt
    98461 ttcgaccatt tttagccact gacttaccct cttgcgtaat tcttttgccg ataattttcc
    98521 tcgctcttct acgtccagta ccgcagggaa atcgccttgt gagaaatcta ccgtttgcag
    98581 aaataatcgc acctgaaccg gtgctgctac cgacggggag aaataatgat atgccccgcg
    98641 cagcaggcca ttttcgcggc ttagttgcca gttacgcgaa aaataggggt ccaccagctt
    98701 ttcgccttcc gtcgccttaa taaaagcaaa ctgtaagcgg ataccgttgt cgcgcatttt
    98761 tgccacccgc tgccagtcga tccgctcctg ccagcgcgaa acatctattc cgtgaatggt
    98821 gtaactggca ggaatgcgaa tagcgaaaga tttaaccggg cgataaccgt agaagtggat
    98881 gtagtctgta atctgaactg ctgtcgaata gaaaaaattc accgtctgac gcggataaat
    98941 tgcaacgatg gaaattagcc caagagcgca taatagagag gtgagttttt ttcgactggt
    99001 aattcttagc tgcattggtt tcatccctga atgtcagtgc caaaggctga caataaccaa
    99061 agcagctata gtacggtgct caccaccagg cgtggaagtg gtgaaccgga ccaataccgt
    99121 gaccaacttc cagcgtgtcg gcctgggcta acgccgatga aagccagctt tttgcctcct
    99181 gtacggtgtc agcccagttt gtatggcgcg ggcgtagtgc agccaacgcc gcagagagtg
    99241 tacaaccagt gccgtgggtg tttttggtca taatgcgcgg tgcggtaaac cgttgttcac
    99301 cctcgcgggt aaacagccag tccgggcttt gctcatcatc cagatgacca cctttcatta
    99361 gcactgcgcc acagcccatc gccaacagcg atcgcccttg ttccagcatt tcctgttcgg
    99421 tgcgcgcgtg tggcgcgtcg agcaaggcgg cagcttcggg caagtttggc gttattaatg
    99481 aaacctgtgg caataatcga ctgcgcagcg tagcaaccgc cgaaggtgaa agcagcgggt
    99541 cgccgctttt tgccagcata acggtgtcga gtaccacgtt ttggatctga taacgttgca
    99601 accgttctgc caccgcttca acaatatcgg tttccgccag cataccgatt ttagtggtat
    99661 cgattcgcac atcgctgaac accgaatcga gctgggcggc gacaaaatca ggctcaatgc
    99721 gatacaccga ctgtacgcca cgggtatttt gcgccaccag tgcagtaata actgagcaac
    99781 cataagcgcc aagtgccgag aaggttttaa gatcggcctg aatccccgca ccaccactcg
    99841 gatcagtacc ggcaatcgtc agagcgttaa ttcgtttcat gcctgcacct cctgcgtcaa
    99901 ttgccagagc gcatcaagga aatgtggaac aaaactgcct ggcccctcgc ttctggcgac
    99961 tgcgcgttct ccggcttgtt tcatccagtg acaggcagat gcgacatttt ccagcgtatc
   100021 gcctggtaac gcacagcagg cagcgacaac cgccgataat gcacagccag ttcctaccac
   100081 tttggtcatt aacggatcac caccgtgaat accaatgata cgatgtccat cggtaacata
   100141 atccatctcg ccagtgacca cgacgattgc gccagtttcc cgtgccagtg tttgtgcagc
   100201 gggtatcgcg ttagctgcgg cgtcagtggt atccactccc cgtccgccat tagcaatgcc
   100261 agctaatgcc atgatttccg aagcattacc acgtatcgct gccggtttaa aagataaaag
   100321 ttcatgacaa aaatggcggc gataatcgag cgcacccacc gctactggat caagcgtcca
   100381 gggtgtttga gagctttttg cttgctcaac ggcagcacgc atcgcctgag cgcgtggctg
   100441 cgtcagtgtg ccaacgttaa tcaacaaggc actggcgata gccgcaaact gactggcctc
   100501 ttcggtttcg ataaccatcg ctggcgatgc accgagcgcc agcaaggtat tggcggtaaa
   100561 ggtttgcacc acatcattgg tcatgcagtg cacaagaggg gaatgttggt gaaaaaggtg
   100621 taacgcgtgc gcagattgcg ctgaacccag caggtcgact tgcatagttt gctcctgcca
   100681 taacgtgaag aagcaatgac ctggtggtcc gtgacttccc tacgctggca ttatccagat
   100741 caggtgatac gggtatttct cagccttcac gcagaagggc accccgagtc gtttggttgc
   100801 gatgataaat cgcagaggag gatggtaatg tccagcgcac gcgttgtaaa cgaggaaaaa
   100861 cggcagtttt acaatcgcgt taataaatta tttgatatat gaatccagca ccttcagaac
   100921 gacatccaga tcttcttcac gttttagctc atccccctgg tgaacgatgt gttccgtcag
   100981 atgaccttta atcacttccc gcatcagacc gtttaccgcg ccacggatag cagcaatctg
   101041 ttgtaaaact gcagcgcatt cgtgcggctc gtcgagcatt ttcttgagcg ccacgacctg
   101101 gccctgaatc ttactggcac gcgctttcag tttctgttta tcacggattg tatgagacat
   101161 ggcaacacct ggttaacaag aatatgaaaa atcatagcac tattaatcta ctggggggta
   101221 gtatcaggta ctggggggga gtagaatcag attgccgaat taatactaag aattattatc
   101281 atgaccgaat ttacaactct tcttcagcaa ggaaacgcct ggttcttcat ccccagcgcc
   101341 atcttacttg gtgcgcttca tggcctggaa ccagggcact caaaaacgat gatggcggcg
   101401 tttatcatcg ccatcaaagg caccattaaa caagcggtga tgctcggact ggcagcaact
   101461 atttcgcata ccgcagtggt ctggttaatt gcctttggcg ggatggtgat cagcaagcgc
   101521 tttactgctc aatcagcaga accgtggctc cagctgattt ccgcagtgat cattattagc
   101581 accgcgttct ggatgttctg gcgtacctgg cgcggcgaac gcaactggct ggagaatatg
   101641 cacgggcatg attatgagca tcatcatcac gatcacgaac atcaccacga ccatggacat
   101701 catcaccatc acgaacatgg cgagtatcag gatgcccatg cacgagccca tgccaatgac
   101761 attaaacgac gctttgatgg tagagaggtc accaactggc aaattttgtt atttggctta
   101821 accggtggcc ttatcccctg cccggcagca attaccgtgc tgttgatatg cattcagttg
   101881 aaagccctga cactgggcgc aacactggtc gtcagtttca gcattggcct ggcgttaacg
   101941 cttgtcaccg taggcgttgg cgcagcaatc agcgttcagc aggtcgcaaa acgctggagc
   102001 ggatttaaca ctctcgctaa acgcgccccc tatttttcca gtctgttgat tggcttagtc
   102061 ggtgtgtata tgggcgtaca tggcttcatg ggcataatgc gataactcaa tcctttaggc
   102121 ttgcatgtta tcttcagccc aggatgattc ttaaatcagc tatttccgct gactctctgc
   102181 tcgctaagga tgcttttagg gcatcctttc atttacactt tttacgaaat catgggatca
   102241 ctaacaaaat atcgcttgtc agttatattg tatggcagga aagatatgcg actgatatta
   102301 cagatcccca aagtggagag tttatgacca ttaaaaataa gatgttgctg ggtgcgcttt
   102361 tgctggttac cagtgccgcc tgggccgcac cagccaccgc gggttcgacc aatacctcgg
   102421 gaatttctaa gtatgagtta agtagtttca ttgctgactt taagcatttc aaaccagggg
   102481 acaccgtacc agaaatgtac cgtaccgatg agtacaacat taagcagtgg cagttgcgta
   102541 acctgcccgc gcctgatgcc gggacgcact ggacctatat gggtggcgcg tacgtgttga
   102601 tcagcgacac cgacggtaaa atcattaaag cctacgacgg tgagattttt tatcatcgct
   102661 aaaaaaatcc ccctcatcat gagggggaaa tgcagacacc ttgttatttt ttattattag
   102721 ccacttgctc gtcttgcttg ttattagtcg tatttcacgt tgattaatgc ggttgcctcc
   102781 aatgcgccag atttaacttt gtttgtatcg tagacgtagt aactggctgt tatcggaatt
   102841 gaaaccgttg tatctgtaag ttttgacttt gccgcaattt cttttaaatt tgtgtatcct
   102901 ttgctgttat cagtgatatt taaaagtgtg actggcgaac cattactttc aagaataaat
   102961 cctacgccat tatcttccgc acctttcagt attgtgttgc tgttggttaa caagtcagaa
   103021 cttgataaat acacctgcat tttccgtgat gtttgatttt gttgacctaa taaattttta
   103081 caacgcagaa taatatcccc ttttttacta tttattgttg ctttgttacc tgtggctggt
   103141 aattgtgaaa cgggaatacc ctctaaagta attaataaat cgtccggttt gcaggttgtg
   103201 gttatcgggt tgtatgacaa ggtcagatta aaggaataga cacctgcatt ttgtgccaat
   103261 gcatttctat agctatttac acatgcatcc catgacttgc cacctaataa acacagtaga
   103321 aattgtactg cggcactccc gcgtgtcgat gatgaagcac tggataatgt cgcaacacca
   103381 ttaatcatca ctgaaccgcc gccccccgca gaaacattca cttttgcatt actggccgca
   103441 ggtgtcaccg ttaccgaata accattggtg aaactggtct gattatttga caacgtgatg
   103501 ttatttttat tggtccattc aaaatgtaaa tttaatttaa tggtatcgtt ataaagtcca
   103561 acaataatat ccgacgggct cataggggtg atagtatttg tactcacgca tgacgaatca
   103621 ttaaaattat gctcatatgt cgccatttgt gtttgagtaa gtgcaaaatc cccctgaaca
   103681 accaagttgt gctgaacaga taactcacta taacatgccg catagctcac aggcataacc
   103741 atcatcatta atataaatag cattatgcga atttccatag aaccacctga ctattactgg
   103801 caaatataat ttctactttc atcaatctct ttaccgaagg tgattgtgca tgaaagtcct
   103861 tgttgcttat caattgctac attaaccgat ggcggtactt cattggtgcg aataaataac
   103921 tggcttccct gaccgacaac gccaatatta tgaccatgga tatcattgac ttcataacca
   103981 aacattaatg gttgcccatc cgctcttaac gcttttataa accagggctt gcgctgatcg
   104041 gtatcaaaat taaccagtac aaccgcgccg cgataagggg cggcaatttt ccggttgcca
   104101 cgtaattctg cttcgctatc gctttgcgac acatccagca tcaggtgatt ttcccgataa
   104161 ggtgtcattc cgtcgtatat caccactcca ttacggtttg ttgtgcgata tttttgccca
   104221 ttgacataag catctttaat tcctggcgca ttcatcacag caaacgtttc ggaaagacgg
   104281 ttcgccagat taacgccacc cgaccaggcg acaatgcccc ctgaaacact ggctccagcc
   104341 tgtcgataag tactcgactg actataactg ccattcactg tcgcaaccgg cgcgttccag
   104401 gtcaaattcg ccccagctgt cgtttcattt ccctgatgtt gatgactcag gttgacacca
   104461 taattgaact gatcccgact ccctactgtt cctgataatc ccgtattatt tgaggcaaac
   104521 ccctgatcat caaacgtcgt tgagttagac atatatattt gccgacgagg cgtcgtaacg
   104581 tcatcacccc aatcaaaggg aatcgatata aaaatattaa atcgtttctc ttcatgatga
   104641 ttctcgtcat aagcctggct tgccgcgagg gtatagctta tccgccgcca gttattggaa
   104701 taactcaact gataatcctt actgctgccg ctacgtcccc agtaatctcg ccataacgta
   104761 cttaaggaca cagaacccca gccttctggc aatgactggc tcatattggc agaaaagcta
   104821 tttttacggc caaaatcgtt ctggtaataa tcggcaatgt catagacatc gttttcatca
   104881 cggcgataat tatctttatt gtttgcccaa acgtgatcat taaatgtccg gtaatcacgc
   104941 gatgaataac gccaggccgc aagaccaaaa cgcgtcgacg tttggctcac aaatttgttg
   105001 taggcaattt gataactttg cccgtcaaac acatcgccgt tgtcttgttt actatgcgac
   105061 ttagtggcat cgacggaaat ggcaccaatg cgtgtattcc agccagtccc caaagtaaac
   105121 gcgtaataat tattcgcgac catcgagccg ccatacagcg tcaataaatt attaaaacca
   105181 tactgataac ccacctggac aaattcactt tgtttgctcg ccccttcaat atggctacga
   105241 cccgccgcaa aatcatattt cgacacgccg ggttgcagca tatttggcac cgctgcataa
   105301 ggcaccagat aggtggttac cgaaccgtcc gcctctttca cgctgacatc aagatctgct
   105361 ccaccaccgg ccaactgcaa atctgtaatc gcgaacgggc caggaggaac ctctttctga
   105421 taaacgacaa aaccattctg ttcaatagtt accagcgcgt tactctgagc aatcccctgc
   105481 acccgtggcg taaaattttg tttcgagtta ggcaacatct gcatatcacg aaacaaccgc
   105541 acacctctga agcgaacaga atcaaaaata tcgcttgatg tgtacatatc acccacgcga
   105601 agcgtgccga gaagttgggc aaatccacgt tccagataca gggtattgct tttccacacc
   105661 cctggattgt tatttgtttt actgaaactg gcatcagaat gcagttgcca ccccagtaaa
   105721 tttaacccgc tgttaaaacg tacatatgta ctcttgttat tacccgacgc tttatagtcg
   105781 ctgtaatact gactcagata ataagaggta taaaacgcat taataccccg ctcccagttt
   105841 tccggtggaa catagccact ttccagttct tccacccagg cctgcgggac actgaaatcg
   105901 agacgaaaaa ccccgatatc ccaggtatag ctcccaccct gaacaagttg ctcaaatgtt
   105961 aaacattgct taccgctggc gaagttatcg ctattaatgc ctaaccgctt gataacttct
   106021 cttgataaac atgtttcttg cgggttgtct ttaacaataa tctcatattt cccgcgccat
   106081 tgcttattga cataaatatc gatgtcatac tgccccggta agggttgatt atcatcaaga
   106141 cgaatatttg caacctgctg gtctttcatt ccacctatca taaaatgggt atcaaaggtt
   106201 tcttcagctg cataagcttc aatgccgagc aataacgcta cgattgctga tgcaagtggg
   106261 gtcattctca acataggcag ctcctgcaat taaattttgt cactaatata gttgccatgg
   106321 tcatcgataa tggtcagatg ccagttattt gcattattac ttttgacatt aacactctga
   106381 ctttctaagg gggcaatcat aatagtttca taattgactt tgacattatt agctttgaca
   106441 tccgaaatcg tcacccaatt agctgagtca ttttttatca ccaaaccatt gccactgcga
   106501 tttaccagca attttttaaa tgtcgcttta tttaccggag caatacccgc tggccggtaa
   106561 aacaacttaa ttctgttttg catcgcaaac ttcagtgcat tcttaccttc ttgctctgga
   106621 ctatttggtg gaatatccag aacattcaga taaaaaatgc tttctttatt agtgggcagt
   106681 ttattcggca taattttgat ttttacttgc tgcccggaat ttgcccctat ttttgccact
   106741 ggtggcgtta acatgaaagg aacctgaatt ttttctggtg gtaatgacgt atcgccatca
   106801 tcaatccacg cctgcagcag cgaagaacgg tttccctggt tcatcaactg caccatcact
   106861 tctttatttt ctgccgggta aataatgcgc gtcccgtaga taacaattcc ggcatgtgca
   106921 ggaaggacca tagaaaaaat gagtaaagat aataatcctt tcattttaat gcctcttaaa
   106981 ttaaaaggcc gccatgggat agcggccatg tttattacct ggagaaactt acaattttat
   107041 ttattaatcc tgcgccacac gcataaccag agaagataac acatcacctg attgcactga
   107101 gttatctgtc agtttcaggt attttgcttt aaacggaata gcatattgac cttccgctgt
   107161 tttataactc tcaatgttaa aaggtgcaga aacatccagg acatcgctgt tatcacctaa
   107221 atagagatag taacccacgg tatcgttgct actggctatc gccttatcgt taccggttat
   107281 tttatcgttg gtaatcaacg agagttttac accagcaact tctttcgggc agctaatttt
   107341 taacatgctg ctggttacac cgactgcatc atcataggta gaatatttag ctttaccttc
   107401 aaagacactg gttggtacag tcggcatatt aatacttact gaatcaccac ccgcttcgaa
   107461 tttacacgga gattctgcaa tattcccctt aatagttaat gttccggtat caacgtctgc
   107521 agcaaatact ccagcgctca taaaaaaaga agaaaagaca gcggcagcaa taattgaacg
   107581 tttcataata tgttccttaa taaaatttat ttttggttgt taagagt
//
LOCUS       DAJVTY010000002        79161 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVTY010000002 DAJVTY010000000
VERSION     DAJVTY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79161
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.002"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            132..1334
                     /gene="proV"
                     /locus_tag="OQQ66_000101"
     CDS             132..1334
                     /gene="proV"
                     /locus_tag="OQQ66_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1903170.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1327..2391
                     /gene="proW"
                     /locus_tag="OQQ66_000102"
     CDS             1327..2391
                     /gene="proW"
                     /locus_tag="OQQ66_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1903171.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2449..3441
                     /gene="proX"
                     /locus_tag="OQQ66_000103"
     CDS             2449..3441
                     /gene="proX"
                     /locus_tag="OQQ66_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1903172.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3733..4917
                     /locus_tag="OQQ66_000104"
     CDS             3733..4917
                     /locus_tag="OQQ66_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1903173.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5041..5778
                     /gene="ygaZ"
                     /locus_tag="OQQ66_000105"
     CDS             5041..5778
                     /gene="ygaZ"
                     /locus_tag="OQQ66_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1903174.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5768..6103
                     /gene="ygaH"
                     /locus_tag="OQQ66_000106"
     CDS             5768..6103
                     /gene="ygaH"
                     /locus_tag="OQQ66_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1903175.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6194..6724
                     /gene="emrR"
                     /locus_tag="OQQ66_000107"
     CDS             6194..6724
                     /gene="emrR"
                     /locus_tag="OQQ66_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1903176.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6851..8023
                     /gene="emrA"
                     /locus_tag="OQQ66_000108"
     CDS             6851..8023
                     /gene="emrA"
                     /locus_tag="OQQ66_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS1903177.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            8040..9578
                     /gene="emrB"
                     /locus_tag="OQQ66_000109"
     CDS             8040..9578
                     /gene="emrB"
                     /locus_tag="OQQ66_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1903178.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9642..10157)
                     /gene="luxS"
                     /locus_tag="OQQ66_000110"
     CDS             complement(9642..10157)
                     /gene="luxS"
                     /locus_tag="OQQ66_000110"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1903179.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10307..11863)
                     /gene="gshA"
                     /locus_tag="OQQ66_000111"
     CDS             complement(10307..11863)
                     /gene="gshA"
                     /locus_tag="OQQ66_000111"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1903180.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCCDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(11936..12364)
                     /locus_tag="OQQ66_000112"
     CDS             complement(11936..12364)
                     /locus_tag="OQQ66_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1903181.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12361..12916)
                     /gene="yqaB"
                     /locus_tag="OQQ66_000113"
                     /pseudo
     CDS             complement(12361..12916)
                     /gene="yqaB"
                     /locus_tag="OQQ66_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
     gene            complement(13064..13140)
                     /locus_tag="OQQ66_000114"
     tRNA            complement(13064..13140)
                     /locus_tag="OQQ66_000114"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13104..13106),aa:Arg,seq:acg)
     gene            complement(13339..13415)
                     /locus_tag="OQQ66_000115"
     tRNA            complement(13339..13415)
                     /locus_tag="OQQ66_000115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13379..13381),aa:Arg,seq:acg)
     gene            complement(13613..13689)
                     /locus_tag="OQQ66_000116"
     tRNA            complement(13613..13689)
                     /locus_tag="OQQ66_000116"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13653..13655),aa:Arg,seq:acg)
     gene            complement(13693..13785)
                     /locus_tag="OQQ66_000117"
     tRNA            complement(13693..13785)
                     /locus_tag="OQQ66_000117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13749..13751),aa:Ser,seq:gct)
     gene            complement(14101..14286)
                     /gene="csrA"
                     /locus_tag="OQQ66_000118"
     CDS             complement(14101..14286)
                     /gene="csrA"
                     /locus_tag="OQQ66_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1903182.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14521..17151)
                     /gene="alaS"
                     /locus_tag="OQQ66_000119"
     CDS             complement(14521..17151)
                     /gene="alaS"
                     /locus_tag="OQQ66_000119"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1903183.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17279..17779)
                     /gene="recX"
                     /locus_tag="OQQ66_000120"
     CDS             complement(17279..17779)
                     /gene="recX"
                     /locus_tag="OQQ66_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1903184.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18022..19083)
                     /gene="recA"
                     /locus_tag="OQQ66_000121"
     CDS             complement(18022..19083)
                     /gene="recA"
                     /locus_tag="OQQ66_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1903185.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19163..19660)
                     /gene="pncC"
                     /locus_tag="OQQ66_000122"
     CDS             complement(19163..19660)
                     /gene="pncC"
                     /locus_tag="OQQ66_000122"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1903186.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19805..20890)
                     /gene="mltB"
                     /locus_tag="OQQ66_000123"
     CDS             complement(19805..20890)
                     /gene="mltB"
                     /locus_tag="OQQ66_000123"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1903187.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21146..21709
                     /gene="srlA"
                     /locus_tag="OQQ66_000124"
                     /gene_synonym="gutA"
     CDS             21146..21709
                     /gene="srlA"
                     /locus_tag="OQQ66_000124"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS1903188.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21706..22665
                     /gene="srlE"
                     /locus_tag="OQQ66_000125"
                     /gene_synonym="gutE"
     CDS             21706..22665
                     /gene="srlE"
                     /locus_tag="OQQ66_000125"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1903189.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITTGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22676..23047
                     /gene="srlB"
                     /locus_tag="OQQ66_000126"
                     /gene_synonym="gutB"
     CDS             22676..23047
                     /gene="srlB"
                     /locus_tag="OQQ66_000126"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1903190.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23051..23830
                     /gene="srlD"
                     /locus_tag="OQQ66_000127"
     CDS             23051..23830
                     /gene="srlD"
                     /locus_tag="OQQ66_000127"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1903191.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLVEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23935..24293
                     /gene="gutM"
                     /locus_tag="OQQ66_000128"
                     /pseudo
     CDS             23935..24293
                     /gene="gutM"
                     /locus_tag="OQQ66_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24360..25133
                     /gene="srlR"
                     /locus_tag="OQQ66_000129"
                     /gene_synonym="gutR"
     CDS             24360..25133
                     /gene="srlR"
                     /locus_tag="OQQ66_000129"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1903192.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKLASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25126..26091
                     /gene="gutQ"
                     /locus_tag="OQQ66_000130"
     CDS             25126..26091
                     /gene="gutQ"
                     /locus_tag="OQQ66_000130"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1903193.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26088..27602)
                     /gene="norR"
                     /locus_tag="OQQ66_000131"
     CDS             complement(26088..27602)
                     /gene="norR"
                     /locus_tag="OQQ66_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1903194.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            27789..29228
                     /gene="norV"
                     /locus_tag="OQQ66_000132"
     CDS             27789..29228
                     /gene="norV"
                     /locus_tag="OQQ66_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1903195.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29225..30358
                     /gene="norW"
                     /locus_tag="OQQ66_000133"
                     /pseudo
     CDS             29225..30358
                     /gene="norW"
                     /locus_tag="OQQ66_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30486..32738)
                     /gene="hypF"
                     /locus_tag="OQQ66_000134"
     CDS             complement(30486..32738)
                     /gene="hypF"
                     /locus_tag="OQQ66_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1903196.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32891..33418)
                     /gene="hydN"
                     /locus_tag="OQQ66_000135"
     CDS             complement(32891..33418)
                     /gene="hydN"
                     /locus_tag="OQQ66_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1903197.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33567..34577)
                     /gene="ascG"
                     /locus_tag="OQQ66_000136"
     CDS             complement(33567..34577)
                     /gene="ascG"
                     /locus_tag="OQQ66_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1903198.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCNAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            34837..36294
                     /gene="ascF"
                     /locus_tag="OQQ66_000137"
     CDS             34837..36294
                     /gene="ascF"
                     /locus_tag="OQQ66_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS1903199.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASADIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36303..37727
                     /gene="ascB"
                     /locus_tag="OQQ66_000138"
     CDS             36303..37727
                     /gene="ascB"
                     /locus_tag="OQQ66_000138"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS1903200.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EVTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37886..38356)
                     /gene="hycI"
                     /locus_tag="OQQ66_000139"
     CDS             complement(37886..38356)
                     /gene="hycI"
                     /locus_tag="OQQ66_000139"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS1903201.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38349..38759)
                     /gene="hycH"
                     /locus_tag="OQQ66_000140"
     CDS             complement(38349..38759)
                     /gene="hycH"
                     /locus_tag="OQQ66_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS1903202.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(38756..39523)
                     /gene="hycG"
                     /locus_tag="OQQ66_000141"
     CDS             complement(38756..39523)
                     /gene="hycG"
                     /locus_tag="OQQ66_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS1903203.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39523..40065)
                     /gene="hycF"
                     /locus_tag="OQQ66_000142"
     CDS             complement(39523..40065)
                     /gene="hycF"
                     /locus_tag="OQQ66_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS1903204.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40075..41784)
                     /gene="hycE"
                     /locus_tag="OQQ66_000143"
     CDS             complement(40075..41784)
                     /gene="hycE"
                     /locus_tag="OQQ66_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1903205.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(41802..42725)
                     /gene="hycD"
                     /locus_tag="OQQ66_000144"
     CDS             complement(41802..42725)
                     /gene="hycD"
                     /locus_tag="OQQ66_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1903206.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(42728..44113)
                     /gene="hycC"
                     /locus_tag="OQQ66_000145"
                     /pseudo
     CDS             complement(42728..44113)
                     /gene="hycC"
                     /locus_tag="OQQ66_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            complement(44099..44359)
                     /locus_tag="OQQ66_000146"
                     /pseudo
     CDS             complement(44099..44359)
                     /locus_tag="OQQ66_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708526.1"
                     /note="regulates several genes involved in the formate
                     hydrogenlyase system; seems to prevent binding of FhlA
                     transcriptional activator to the activator sequence of hyc
                     operon; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            44571..44921
                     /gene="hypA"
                     /locus_tag="OQQ66_000147"
     CDS             44571..44921
                     /gene="hypA"
                     /locus_tag="OQQ66_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1903207.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            44925..45797
                     /gene="hypB"
                     /locus_tag="OQQ66_000148"
     CDS             44925..45797
                     /gene="hypB"
                     /locus_tag="OQQ66_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1903208.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            45788..46060
                     /gene="hypC"
                     /locus_tag="OQQ66_000149"
     CDS             45788..46060
                     /gene="hypC"
                     /locus_tag="OQQ66_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1903209.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            46060..47181
                     /gene="hypD"
                     /locus_tag="OQQ66_000150"
     CDS             46060..47181
                     /gene="hypD"
                     /locus_tag="OQQ66_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1903210.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            47178..48188
                     /gene="hypE"
                     /locus_tag="OQQ66_000151"
     CDS             47178..48188
                     /gene="hypE"
                     /locus_tag="OQQ66_000151"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1903211.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            48262..50340
                     /gene="flhA"
                     /locus_tag="OQQ66_000152"
     CDS             48262..50340
                     /gene="flhA"
                     /locus_tag="OQQ66_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1903212.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(50377..50730)
                     /locus_tag="OQQ66_000153"
     CDS             complement(50377..50730)
                     /locus_tag="OQQ66_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1903213.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(50807..50941)
                     /locus_tag="OQQ66_000154"
     CDS             complement(50807..50941)
                     /locus_tag="OQQ66_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903214.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            51017..53578
                     /gene="mutS"
                     /locus_tag="OQQ66_000155"
     CDS             51017..53578
                     /gene="mutS"
                     /locus_tag="OQQ66_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1903215.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            53684..54340
                     /gene="pphB"
                     /locus_tag="OQQ66_000156"
     CDS             53684..54340
                     /gene="pphB"
                     /locus_tag="OQQ66_000156"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCS1903216.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(54391..55158)
                     /locus_tag="OQQ66_000157"
     CDS             complement(54391..55158)
                     /locus_tag="OQQ66_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1903217.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGLSTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            55354..56262
                     /locus_tag="OQQ66_000158"
     CDS             55354..56262
                     /locus_tag="OQQ66_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1903218.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            56259..57520
                     /locus_tag="OQQ66_000159"
                     /pseudo
     CDS             56259..57520
                     /locus_tag="OQQ66_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            57517..58154
                     /locus_tag="OQQ66_000160"
                     /pseudo
     CDS             57517..58154
                     /locus_tag="OQQ66_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
     gene            58159..58935
                     /locus_tag="OQQ66_000161"
     CDS             58159..58935
                     /locus_tag="OQQ66_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1903219.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAMEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            59024..60388
                     /locus_tag="OQQ66_000162"
     CDS             59024..60388
                     /locus_tag="OQQ66_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS1903220.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(60482..61474)
                     /gene="rpoS"
                     /locus_tag="OQQ66_000163"
     CDS             complement(60482..61474)
                     /gene="rpoS"
                     /locus_tag="OQQ66_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1903221.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(61537..62628)
                     /gene="nlpD"
                     /locus_tag="OQQ66_000164"
     CDS             complement(61537..62628)
                     /gene="nlpD"
                     /locus_tag="OQQ66_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1903222.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKTAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(62768..63394)
                     /gene="pcm"
                     /locus_tag="OQQ66_000165"
     CDS             complement(62768..63394)
                     /gene="pcm"
                     /locus_tag="OQQ66_000165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1903223.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(63388..64149)
                     /gene="surE"
                     /locus_tag="OQQ66_000166"
     CDS             complement(63388..64149)
                     /gene="surE"
                     /locus_tag="OQQ66_000166"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1903224.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(64130..65179)
                     /gene="truD"
                     /locus_tag="OQQ66_000167"
     CDS             complement(64130..65179)
                     /gene="truD"
                     /locus_tag="OQQ66_000167"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1903225.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(65176..65655)
                     /gene="ispF"
                     /locus_tag="OQQ66_000168"
     CDS             complement(65176..65655)
                     /gene="ispF"
                     /locus_tag="OQQ66_000168"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1903226.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHIDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(65655..66365)
                     /gene="ispD"
                     /locus_tag="OQQ66_000169"
     CDS             complement(65655..66365)
                     /gene="ispD"
                     /locus_tag="OQQ66_000169"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1903227.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(66384..66695)
                     /gene="ftsB"
                     /locus_tag="OQQ66_000170"
     CDS             complement(66384..66695)
                     /gene="ftsB"
                     /locus_tag="OQQ66_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1903228.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(66889..67212)
                     /locus_tag="OQQ66_000171"
     CDS             complement(66889..67212)
                     /locus_tag="OQQ66_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1903229.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(67262..67867)
                     /gene="cysC"
                     /locus_tag="OQQ66_000172"
     CDS             complement(67262..67867)
                     /gene="cysC"
                     /locus_tag="OQQ66_000172"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1903230.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(67867..69294)
                     /gene="cysN"
                     /locus_tag="OQQ66_000173"
     CDS             complement(67867..69294)
                     /gene="cysN"
                     /locus_tag="OQQ66_000173"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1903231.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(69296..70204)
                     /gene="cysD"
                     /locus_tag="OQQ66_000174"
     CDS             complement(69296..70204)
                     /gene="cysD"
                     /locus_tag="OQQ66_000174"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1903232.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            70456..71493
                     /gene="iap"
                     /locus_tag="OQQ66_000175"
     CDS             70456..71493
                     /gene="iap"
                     /locus_tag="OQQ66_000175"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1903233.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(71700..71993)
                     /gene="cas2"
                     /locus_tag="OQQ66_000176"
     CDS             complement(71700..71993)
                     /gene="cas2"
                     /locus_tag="OQQ66_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS1903234.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(71993..72913)
                     /gene="cas1e"
                     /locus_tag="OQQ66_000177"
     CDS             complement(71993..72913)
                     /gene="cas1e"
                     /locus_tag="OQQ66_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCS1903235.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(72910..73560)
                     /gene="cas6e"
                     /locus_tag="OQQ66_000178"
     CDS             complement(72910..73560)
                     /gene="cas6e"
                     /locus_tag="OQQ66_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1903236.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(73542..74288)
                     /gene="cas5e"
                     /locus_tag="OQQ66_000179"
     CDS             complement(73542..74288)
                     /gene="cas5e"
                     /locus_tag="OQQ66_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS1903237.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(74299..75377)
                     /gene="cas7e"
                     /locus_tag="OQQ66_000180"
                     /pseudo
     CDS             complement(74299..75377)
                     /gene="cas7e"
                     /locus_tag="OQQ66_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(75392..75960)
                     /gene="casB"
                     /locus_tag="OQQ66_000181"
                     /pseudo
     CDS             complement(75392..75960)
                     /gene="casB"
                     /locus_tag="OQQ66_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            complement(75957..77519)
                     /gene="casA"
                     /locus_tag="OQQ66_000182"
     CDS             complement(75957..77519)
                     /gene="casA"
                     /locus_tag="OQQ66_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCS1903238.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(77617..79161)
                     /gene="cas3"
                     /locus_tag="OQQ66_000183"
                     /pseudo
     CDS             complement(77617..79161)
                     /gene="cas3"
                     /locus_tag="OQQ66_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        18652 a        21495 c        20245 g        18769 t
ORIGIN      
        1 tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct cagtatgtta
       61 gggtagaaaa aagtgactat ttccattggg taatatatcg acataaacaa ataaaggaat
      121 ctttctattg catggcaatt aaattagaaa ttaaaaatct ttataaaata tttggcgagc
      181 atccacagcg agcgttcaaa tatatcgaac aaggactttc gaaagaacaa attctggaaa
      241 aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat
      301 ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc
      361 tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc aaaatatccg
      421 atgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct
      481 taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg gccggaatta
      541 atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt
      601 atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc
      661 gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg gcgctcgatc
      721 cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca
      781 ccattgtctt tatttcccac gatctcgatg aagctatgcg tattggcgac cgaattgcca
      841 ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc aataatccgg
      901 cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggta ttcagtgcga
      961 aagatattgc ccgccggaca ccgaatggct taattcgtaa aacccctggc ttcggcccac
     1021 gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt atcgaacgcg
     1081 gtaataagtt tgtcggcgca gtttccatcg attcgcttaa aaccgcgtta acgcagcagc
     1141 aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa acgcctctta
     1201 gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt acccgtggtc gacgaggacc
     1261 aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg
     1321 taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgccgtg
     1381 caatccgcag acgcctgggg tacaccggcg actgcaccga ctgacggcgg tggtgctgac
     1441 tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat
     1501 aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt tgttacccat
     1561 ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag
     1621 caattgctgc tgggtatgcc cgcaccggtg gcgattatcg ttttcgctct catcgcctgg
     1681 cagatttccg gggtcggaat gggcgtggcg acgctggttt cgctgattgc catcggcgca
     1741 atcggtgcct ggtcgcaggc catggttacc ctggcgctgg tgttaaccgc cctgctgttc
     1801 tgtatcgtca ttggtttgcc gctggggatc tggctggcga gaagtccgcg agcggcgaaa
     1861 attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta tctggtgcca
     1921 atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat catctttgct
     1981 ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc ggatctgatt
     2041 gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg
     2101 ctggcgatgc cgagcattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg
     2161 gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact tcgcggtatc
     2221 ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt
     2281 atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc
     2341 tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta actctgcact
     2401 tgcccggtga cgccgggcat tatcaccctg ccaaaaaaag gaataacaat gcgacatagc
     2461 gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc tgccgatctg
     2521 ccgggcaaag gtattactgt taatccagtt caaagcacca tcaccgaaga aaccttccag
     2581 acgctgctgg tcagccgtgc gctggagaaa ttaggctata ccgtcaacaa acccagcgaa
     2641 gtagattaca acgttggcta caccttgctt gcttccggcg atgcaacctt caccgccgtg
     2701 aactggacgc cactgcatga caacatgtac gaagctgccg gtggcgataa gaaattttat
     2761 cgtgaagggg tatttgttaa cggcgcggca cagggttacc tgatcgataa gaaaaccgcc
     2821 gaccagtaca aaatcaccaa catcgcacaa ctgaaagagc cgaagatcgc caaactgttc
     2881 gataccaacg gcgacggtaa agcggattta accggttgta atcctggctg gggctgcgaa
     2941 ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac gcataatcag
     3001 gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg caaaccggtg
     3061 ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg gaaagatgtg
     3121 gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga taccaaactg
     3181 ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc caacaaagcc
     3241 tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt gccagtggcc
     3301 gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg cgatattcag
     3361 ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg ggtgaatgag
     3421 gcgctggcag cgcagaagta atttttattc aggcggataa ggcgtttttg ccttatccgc
     3481 cgctctgtac acaatgcctg atgcgacgct gacgcgtctt atcatgccta caaaatacac
     3541 tcattccgta tggcggataa ggcgttttcg ccgcatccgc cgttctgtgc acaatgcctg
     3601 atgcgacgct ggcgcgtctt atcatgccta caaaccacat cgcacatttc aacaagctat
     3661 ctttcatccc atattcatca acatccgcta ttattgattt ccagcttaat catcacctga
     3721 tgaacaaaaa taatgactaa acctaatcat gagcttagcc cggcgctgat cgtgctgatg
     3781 tctatcgcca ccggtctggc ggtagccagt aactattacg cccagccatt gctcgacacc
     3841 atcgcgcgta acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcaa
     3901 ttgggctatg cagcaggtct gctgtttctt gttcccctcg gtgatatgtt tgaacgccgc
     3961 cgcctgattg tctcgatgac cttactggcg gcaggcggca tgttaatcac tgccagcagt
     4021 cagtcgctgg cgatgatgat cctcggtacg gcattaaccg gtttattctc tgtcgtggca
     4081 caaattctgg ttccgctggc agcgacgctg gcttcaccgg acaaacgcgg caaagtggtt
     4141 ggcactatta tgagcggtct gctgttgggg atcttactcg cacggaccgt tgccggattg
     4201 ctggcgaatc tcggcggctg gcgcaccgtc ttttgggttg cctcggtgtt aatggcactg
     4261 atggcgctgg cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaactac
     4321 ccacagttgt taggttccgt tttcagcatg tttatcagcg ataagatcct gcgcacccgc
     4381 gcattgctgg gctgcctgac ctttgccaac ttcagcattc tctggacctc aatggccttt
     4441 ttgcttgccg ctccaccttt taactacagc gatggtgtca ttggtctgtt tggacttgcg
     4501 ggagctgccg gagcgttggg cgctcgtccg gcgggcggtt ttgccgataa gggtaaatcg
     4561 caccacacca caactttcgg tctgctgctg ctattacttt catggctggc gatctggttt
     4621 ggtcacactt ccgtactggc gctgattatc ggcatcctgg tgctggatct caccgtacag
     4681 ggcgtgcata tcactaacca gacggtaatt tatcgaatac accctgatgc gcgtaatcgc
     4741 ctgaccgcag gttacatgac cagctacttt attggcggtg ccgccggttc gctaatttca
     4801 gcctcagcct ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc gacgattgcc
     4861 ctggttaact tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca
     4921 atcagcaacc gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga
     4981 taaggttaag atattcattc agtctattta taatattaac aatcgttaag cgtacactct
     5041 atggaaagcc ctactccaca gcctgctctt ggttcggcga ccttcatgga aggatgcaaa
     5101 gacagtttac cgattgttat tagttatatt ccggtggcct ttgcgttcgg tctgaatgcg
     5161 acccgtctgg gattctctcc tctcgaaagc gtttttttct cctgcatcat ttatgcaggc
     5221 gcgagccagt tcgtcattac cgcgatgctg gcagccggga gtagtttgtg ggttgctgca
     5281 ctgaccgtca tggcaatgga tgttcgccat gtgttgtatg gcccgtcact gcgtagccgt
     5341 attattcagc gtctgcaaaa atcgaaaacc gccctgtggg catttggcct gacggatgag
     5401 gtttttgccg ccgcaaccgc aaaactggta cgcaataatc gccgctggag cgagaactgg
     5461 atgatcggca ttgccttcag ttcatggtca tcgtgggtat ttggtacggt aataggggca
     5521 ttctccggca gcggcttgct gcaaggttat cccgccgttg aagctgcatt aggttttatg
     5581 cttccggcac tctttatgag tttcctgctc gcctctttcc agcgcaaaca atctctttgc
     5641 gttaccgcag cgttagttgg tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc
     5701 attctggcag gcattgtctg tggctgcctc actgcgttaa tccaggcatt ctggcaagga
     5761 gcgcccgatg agctatgagg ttctgctgct tgggttactg gttggcgtgg cgaattattg
     5821 cttccgctat ttgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc
     5881 ggtaggtatt ttgctcgaca ccattggcat cgcctcgata tgcgctctgc tggttgtctc
     5941 taccgcacca gaagtgatgc acgatacacg ccgtttcgtg cccacgctgg tcggcttcgc
     6001 ggtactgggt gccagtttct ataaaacacg cagcattatc atcccaacac tgcttagtgc
     6061 gctggcctat gggctcgcct ggaaagtgat ggcgattata taacccacaa gaatcatttt
     6121 tctaaaacaa tacatttact ttatttgtta ctgtcgttac tatatcggct gaaattaatg
     6181 aggtcatacc caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc
     6241 cagccgccac gaagattttc cttatcagga gatccttctg actcgtcttt gcatgcacat
     6301 gcaaagcaag ctgctggaga accgcaataa aatgctgaag gctcagggga ttaacgagac
     6361 gttgtttatg gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga
     6421 attaagttgt gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga
     6481 aaaacgcggt tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca
     6541 attaacggaa aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc ataactgcct
     6601 gcatcaactc tggtccgcgc tcagcacaac agaaaaagat cagctcgagc aaatcacccg
     6661 caaattgctc tcccgtctcg accagatgga acaagacggt gtggttctcg aagcgatgag
     6721 ctaacgcgtc atctcgctca aaaatccaga tttataaaag aaaaaatgac cggccagcat
     6781 cgcaacatgc tggccttttt ggcaagcagg tcggctcagc cgatgagtta agaagatcgt
     6841 ggagaacaat atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg taaagaagag
     6901 cggcaaacgt aagcgtctgc tcctccttct caccttgctc tttataatta ttgccgtagc
     6961 gatagggatt tattggtttt tggtactgcg tcatttcgaa gaaaccgatg acgcatacgt
     7021 ggcagggaat cagattcaaa ttatgtctca ggtgtctggc agcgtgacga aagtctgggc
     7081 cgataacacc gattttgtaa aagaaggcga tgttctggtc actctcgacc cgacagatgc
     7141 tcgccaggcg tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc aaacccacca
     7201 gctgatgatt aacagcaaac agttacaggc gaatattgag gtgcaaaaaa tcgccctcgc
     7261 gaaagcacaa agcgactaca accgccgtgt gccgctgggc aatgccaacc tgattggtcg
     7321 cgaagagctg caacacgccc gcgacgccgt caccagtgcc caggcgcaac tggacgtcgc
     7381 tattcaacaa tacaatgcca atcaggcgat gattctgggg actaaactgg aagatcagcc
     7441 tgccgtgcaa caggctgcca ccgaagtacg taacgcctgg ctggcgctgg agcgtacccg
     7501 tattgtcagt ccgatgaccg gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat
     7561 tagcccaacg acgccgctga tggcggtcgt tccagccacc aatatgtggg tggatgccaa
     7621 ctttaaagag acgcagattg ccaatatgcg tatcggtcag ccggtcacta tcaccacgga
     7681 tatttacggc gatgatgtga aatacaccgg taaagtggtt ggtctggata tgggcacagg
     7741 tagcgcattc tcactgcttc cggcgcaaaa tgcgaccggt aactggatca aagtcgttca
     7801 gcgtctacct gtgcgtatcg aactggacca gaaacagctg gagcaatacc cgctgcgcat
     7861 tggtttgtcc acgctggtga gcgtcaatac cactaaccgt gacggtcagg tactggcaaa
     7921 taaagtacgt tcaacaccgg tcgcggtaag taccgcgcgt gaaatcagcc tggcacctgt
     7981 caataaactg atcgacgata tcgtaaaagc taacgctggc taatccagag gtgcgtgtga
     8041 tgcaacagca aaaaccgctg gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac
     8101 tggcgacatt catgcaggtg ctggactcca ccattgctaa cgtggcgatc cccactatcg
     8161 ccgggaatct gggctcatcg ctcagccagg gaacgtgggt aatcacttct ttcggggtgg
     8221 cgaatgccat ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac
     8281 tgttcctttg gtccaccatc gcctttgcta ttgcgtcgtg ggagtgtggt gtctccagca
     8341 gcctgaatat gctgatcttc ttccgtgtga ttcaggggat tgtcgctggg ccgttgatcc
     8401 cactttcgca aagtcttttg ctgaataatt acccgccagc caaacgctca atcgcgctgg
     8461 cgttgtggtc gatgacggtg attgtcgcgc caatttgcgg cccaatcctc ggcggttata
     8521 tcagcgataa ttaccactgg ggctggatat tcttcatcaa cgtgccaatt ggcgtggcgg
     8581 tggtgttgat gacactgcaa accctgcgcg gacgcgaaac ccgcaccgaa cggcggcgga
     8641 ttgatgccgt ggggctggca ctgctggtta ttggtatcgg tagcctgcag attatgctcg
     8701 accgcggtaa agagctggac tggttttcat cacaggaaat tatcattctt accgtggtgg
     8761 cggtggtggc tatctgcttc ctgattgtct gggagctgac cgacgataac ccgatagtcg
     8821 atctgtcgtt gtttaagtcg cgcaacttca ccatcggctg cttgtgtatc agcctcgcgt
     8881 atatgctcta cttcggcgcg attgttctgc tgccgcagtt gttgcaggag gtctacggtt
     8941 acacggcgac ctgggcaggt ttggcctctg cgccggtagg gattattccg gtgatcctgt
     9001 cgccgattat cggccgcttc gcgcataaac tggatatgcg gcggctggta accttcagct
     9061 ttattatgta tgccgtctgc ttctactggc gtgcctatac ctttgaacca ggtatggatt
     9121 ttggcgcgtc ggcctggccg cagtttatcc aggggtttgc ggtggcctgc ttctttatgc
     9181 cgctgaccac cattacgctg tctggtttgc caccggaacg actggcggcg gcatcgagcc
     9241 tctctaactt tacgcgaacg ctggcggggt ctatcggcac gtcgataacc acgaccatgt
     9301 ggaccaaccg cgagtcgatg caccatgcgc agttgactga gtcggtaaac ccgttcaacc
     9361 cgaatgccca ggcgatgtac agtcaactgg aagggcttgg gatgacgcaa cagcaggcat
     9421 caggctggat tgcccagcag atcactaatc aggggctgat tatttccgcc aatgagatct
     9481 tctggatgtc agccgggata ttcctcgtcc tgctggggct ggtgtggttt gctaaaccgc
     9541 cctttggcgc aggtggcggc ggaggtggtg cgcactaagt acaactaagc cagttcattt
     9601 gaactggctt ttttcaatta attgtgaaga tagtttactg actaaatgtg cagttcctgc
     9661 aacttctctt tcggcagtgc cagttcttcg ttgctgttga tgcgtacgtc acgttccaga
     9721 atgctacgcg caatatcctg cgcttcctgc aacgagtgca tctggtaagt gccacactgg
     9781 tagacgttca gctccgggat ctgattctga tcctgcactt tcagcacgtc ttccattgcc
     9841 gctttccagg catcagcaac acgctgctca tctggcgtac caatcagact catataaaaa
     9901 ccggtgcggc agcccattgg cgagatatcg ataatctcta caccattacc gttaagatgg
     9961 ttacgcataa aaccagcaaa caggtgctcc agagtatggc tccctctttc tggcatcact
    10021 tctttgttcg gcacgcagaa gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg
    10081 ttcattgttt tcgccacccg aactgcaggc gcttccatcc gggtatgatc gactgtgaag
    10141 ctatctaaca acggcattta gccacctccg gtaatttttt taaaaatttt ctgaactctt
    10201 tcttcccagg cgagtctgag tatatgaaag acgcgcattt gttatcatca tccctgaatt
    10261 cagagatgaa attttggcca ctcacgagtg gcctttttct tttctgtcag gcgtgttttt
    10321 ccagccacac cgcaaacggt tcggtatcag cggtttccat ttcctgctga cggcgttcag
    10381 atgcctcgcg ctcggctaca aaatcctctt cgcgcagaat ttccaacggc tcttcacgca
    10441 gcagattacg gtaggcttct gcaaacgctt tgcctgttcc gccaatacca gtatcaatca
    10501 tagaccttaa gatacgggca gagaaagtca gatcgggatt atcgaagcag gcgaccagtt
    10561 catcacacac tttctgatac gctttgccgc cgttaatgct atccaacgtt tgcgcgacgc
    10621 gtttcagatc gcggaacaga tctttaccca cctgcggtaa cgggaactgc gcggtttcgc
    10681 agccgatacc cagcgtcagg cccggtttgc gaccttcgag gatcacccgg ttccagttaa
    10741 cgcgtgtaca ggcaagttcg ctactgctca tttccggtgc atcagccagc gcacaccaga
    10801 ccataaacag gtcgaggaat cgcacctgct gttcatctac accaatcggc gagaacgggt
    10861 tgatgtccag cgaacgcact tcaatatatt caatgccgcc acgtaacagc gcatcagaag
    10921 gtgactcgcc gctgcgggta acgcgttttg gacgaatcgg cgcatacagt tcgttttcaa
    10981 tctgcagcac gttgctattg atttgcagcc tcttaccgtc tttctcaata ccaatcttcg
    11041 cgtactcttc cgatggcgtt ttgattgcct gtttaaggcc cgctacgtac tcgtaaagat
    11101 cgttgaaggt aataccaaga ttgctttgcg atttattggt atagccgaga tcgctcaaac
    11161 gaagagaggt cgcatacggc aggtaataca taccgcactc ggttttctca aacggcagcg
    11221 acgttggttt tccttgcagg aaagaagaac aaatcgccgg agatgcacca aacagataag
    11281 gaatgaccca accgaaacga tagtaattgc ggataacgcg gaaatagccc gcagaaattt
    11341 tctctttggc atcagtgccc gagatatcac agcacttcgc ttgccagaat gccattggca
    11401 aagagaaatt gtagtgcacg ccggaaatgg tttgcatcag cgcgccgtag cgatttttca
    11461 gcccttcacg atacagcgtt ttaaagcgtc cggtgttaga agtgccgtac tgtgccagtt
    11521 cgatgtcctg accttctgcg atgtagcatg gcatacttaa cggccacatc cgctcatcgc
    11581 ccatattgcg cgccgtataa cgatgcagat cgcgcataaa ggtcaacata tgttcaatat
    11641 caccatccac tggtgtaatg aattccagca atgcttccgc aaaatcggta gtaatccatt
    11701 tgtgcgtcag tgcggaacct aatgcttcag gatgacctgt tgttgcctgt gtgccatcag
    11761 cattaacacg caaagtttcg cgctccagcc cacgctgtat cccctttaac gcctgaggat
    11821 gtttttccag ccaggccagc gcctgtgata cgtccgggat caaattgacc tcccgcctgt
    11881 caaaatcgtt ttaattagca taactgtaat ggtgaccata tgtgcaggcc tacaattagt
    11941 gccaccacat catgccctga acggtagctg ctgcaactgc aacatagcgt aaagctttac
    12001 caaggcacaa aaaaaagatt accggtcccc acgagatgcg catccatccc gctaacagac
    12061 acagtaaatc gccaaccacg ggcatccagc ttaataatag cgtgactgca ccatagcgtt
    12121 tcagccagcc agtagctttc tcttgccagc gcgatgtttt acgcaatgga aagaaacgcc
    12181 caaggataac gttagttaac cctccaaggc tattacccat tgttgctgtt aaaactaaaa
    12241 cccagggata actgatcccg gaaagcaaca ttgccaccag cacgacttcg gagttgccgg
    12301 gtaatagtgt agcgctgaga aaactactgg caaacaacga gaaaagcgat aacgcttcac
    12361 tcacagcaag cgaacatcca cggcgtccat gcctgctgca cgggccgcct gaataccgaa
    12421 atcggcatct tcaaagacca cacactgcgt cggttgcacg cccatacgct gcgcgcacaa
    12481 caaaaatgtg tctggcgcgg gtttatggtg tttgacgtga tcagcagcga cgacggcgtc
    12541 aaaataacgg cgtaatccca ggtgcgccag caatgcctca gcgatggcgc tttcactccc
    12601 cgttcctaca gccattgggc gacgaccatg ccaacttttc accacttcaa caagaggaag
    12661 cggctcgaca ctatccagca gcatacttct tactgcttct gttttttcac gcgctaacgc
    12721 atgcggggcg agatcggcct gattcagctc aataattgcc tgagcaatac gccaggtggg
    12781 cgatccatta agcgcaatca ttgcctgaac atcgtactga agaccgtagt gccctaatac
    12841 ttcgcgccac gctttacggt gcgtaggctc cgtatccagg aatatcaaaa attaaacctg
    12901 cataacgctc gtacatggtc ttctcgcaag tcgaaaaata tgacgctact ttagcgtaat
    12961 tgcttgattt tgtcgctgat ggcgaaggag aagaaaattc aggaaatgga taaagtagtt
    13021 atttagaaat gagatatttt tgaaggaaat ttttgggaga agatggtgca tccgggagga
    13081 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
    13141 atcgggaaaa tttattttac tgctgatatt gatatcgcta ctaaagccat atcaagtaag
    13201 agatggtgca tccgggagga ttactcgcct tgcggctcgc ccttcgggcc gttgctaaag
    13261 caacgttatc atccctggtg cctgcggtta attccgctac ctcttcgata ccttcattgc
    13321 tgaagatttc ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    13381 tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaacttact ttactgcaga
    13441 ttttttgata ccgctactaa agcggtatca agtaagagat ggtgcattcg ggaggattac
    13501 tcgccttgcg gctcgccctt ctggccgttg ctaaagcaac gttatcctcc ctggtgcctg
    13561 cggttatacc gccacctctt ccataccttc atcgctgaaa gtacgtagaa tatggtgcat
    13621 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
    13681 tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt ttgaccgcat
    13741 actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg cctcttacga
    13801 gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata ttactttctg
    13861 agacttataa gtcaaacaat ttttcccaca cttttatcgt ttgcacactt cacgctcaat
    13921 tagtctgtaa aaacgtcaaa aagagtgttt tatctacaga aaaacggagg tctggcagat
    13981 agcagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag cgaaaagact
    14041 atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga cgcggaaaga
    14101 ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct cttcacggtg
    14161 aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct tcacccctaa
    14221 aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac gagtcagaat
    14281 cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc ccggcattat
    14341 ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag cagtcatggc
    14401 attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg actttatcgt
    14461 tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct gacgcttata
    14521 ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag gtaaggccgc
    14581 agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc cgcccacctg
    14641 ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt ccttagatac
    14701 gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa ttgtcgaccc
    14761 cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa caccgctaag
    14821 ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa gatttgcgct
    14881 ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact gacgcgtacg
    14941 ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca gcagatgcgc
    15001 gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt ctccggttac
    15061 cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc ggaacagacc
    15121 aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga aatcgcccat
    15181 gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg cacctttcgc
    15241 tttcgccgct tcgagatcca tgatgttggt ttcgatcggc aaattgcgac gaatctgtgc
    15301 gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt tgtgtgagaa
    15361 gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat gagtccccag
    15421 aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac gaatacgggc
    15481 gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc actttcagag aacccgcagc
    15541 aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca ccgcgaagga
    15601 gaagttagcg tctttcagtt cgcctttatc accaacctga ccgccggatt ccgcatagaa
    15661 tggtgtttga tctagcacga ccacagcttc ctggcctgca ttgatggcat caaccgcttt
    15721 accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat agcctttaaa
    15781 ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc cgctggcttc
    15841 gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt caactttgat
    15901 gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg tgtcgtacag
    15961 acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct cttcatccag
    16021 caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct tcagcacctg
    16081 ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa cgtcgatcag
    16141 cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc cgtgacgcac
    16201 tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg gcattacgcc
    16261 atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca gcgatttatt
    16321 gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc ggaacaggtc
    16381 gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca gacccatacc
    16441 ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc ggttgaactg
    16501 catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg gacttcccgg
    16561 agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg ggccgcacgg
    16621 accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt tgttatcgcc
    16681 gatgcgaata atacgttcgc gcgggatccc gacttctttt tcccagattt cgtaggcttc
    16741 gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa accatttttc
    16801 gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt cgccgaagct
    16861 gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga cgttttccag
    16921 gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc gggaataatt
    16981 acgcttgtcg agcccaagga acacatcctt gaactgattc atcccggcgt tggtaaacaa
    17041 caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat gtcccttact
    17101 atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca taattatcct
    17161 gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc cgggctggta
    17221 actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc gtatgcgttc
    17281 agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca cgatagagca
    17341 gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca tcatattttc
    17401 gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc atcgcttttt
    17461 ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt gcaggtccat
    17521 aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca tcgagatagc
    17581 catgttcatg gcaccatgca ataacgcgct cgtaatcttc tgccgtagca tcaatctctt
    17641 ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct tgctcactgt
    17701 gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat gccgggcgac
    17761 gggacgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag ggccgcagat
    17821 gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtgtgcag aatgttcccg
    17881 caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt gctggcctgt
    17941 tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca cacattatct
    18001 attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc cttcgctgtc
    18061 atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac gtactttctt
    18121 ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat tcgctttacc
    18181 ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga tcagcttctc
    18241 ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt agaggatctg
    18301 aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca cgcgggtttc
    18361 gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt cgagacgaac
    18421 agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac cgaacatcac
    18481 accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact gcttcaggtt
    18541 acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca tgtgagagtc
    18601 gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt caacgacgat
    18661 aacgtctact gcaccagaac gcgccagggc gtcacagatt tccagtgcct gctcgccggt
    18721 gtccggctgg gagcacagca ggttgtcgat atcgacgccc agtttacgtg cgtagattgg
    18781 gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct gcgctgcggc
    18841 gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga tttcgacgat
    18901 acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg aaccggtaga
    18961 gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc ctttaccaaa
    19021 ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt cgtcgatagc
    19081 catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg ttgaagtaat
    19141 tatactgtat gttcatacag tatcaagtgt tttgtagaaa ttgttgccac aaggtctgca
    19201 atgcatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag cattcccgcc
    19261 gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg acaggcttct
    19321 cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa tcagcacgag
    19381 ccgctttcag tgcgcctatc accatttcca ccacgacggg ttcactcacc gcgccatgct
    19441 gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta ctgtaggtga
    19501 caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact ttcgctaccc
    19561 aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc agcgcctgcc
    19621 caacctgttc acttaactgc atcagttcac tgtcagtcat cacaacctct gtcagtcaaa
    19681 aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt tgcaatttta
    19741 cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc cctcttgcaa
    19801 atagttactg cacgcgcgcc agcgccacgg cttgtcctaa ctgccagacc gccattgcgt
    19861 aatgggtgct gtggttataa cgggtaatgg tgtagaagtt cggcagaccg taccagtact
    19921 ggtaaccggt accgacatcc agacgcagca ggctggcttg ttgatggttg cccagcggct
    19981 gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct gtacctggtt ttgaagccat
    20041 ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac ctgatcgcct ttcacccagc
    20101 cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc cacaggttga
    20161 tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc ataaactgtc
    20221 cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc tgctcgtcgc
    20281 gcgccatcag caggaaggtt tccagctcgc cagagaaata ctccgcgcgg cgtgggtagt
    20341 taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg cgcccccagc
    20401 gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac acctgccacg
    20461 cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc acgttgtccg
    20521 gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt cggacctgat ggcggtttca
    20581 ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga cgcttcgcct
    20641 gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc attttgtcga
    20701 tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc acattgtgct
    20761 gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc tcagtaggtt
    20821 ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt acataacgac
    20881 gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg taaagcatca
    20941 cgccccgcac aaggaagcgg tagtcactgc ccgatacgga ctttacataa ctcaactcat
    21001 tcccctcgct atccttttac tcaaacttcc aaattaaaat atttatcttt catttttaga
    21061 ttaaaataac acttttaaat ctttcaatct gattagatta ggttgccgtt tggtaataaa
    21121 acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg cagagtggtt
    21181 tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg gcattcttcc
    21241 gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg gtcagcatcg
    21301 tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc tgctgctacc
    21361 gtgcattggc acgtttgtct tttgcaatcc gatgacctta agccagggtc gctttatgcc
    21421 ggaaaagtac aaacccagct actacgcggc ggcctcttat agctgccact caatgaatgg
    21481 cctctttccc catatcaacc ctggcgaact gtttgtttat cttggcattg ccagcggtct
    21541 gacaacgctg aacctgccac ttggcccact ggcggtgagc tatctgctgg ttggtctggt
    21601 caccaacttc tttcgcggct gggtgaccga tctgaccacc gccatttttg agaaaaagat
    21661 gggcattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac gcgtattcgg
    21721 atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tggaagccac gccgggcaaa
    21781 aaaatcgtct atatcaccac cggtacccgg cctgcgattg ttgacaaact ggcacaactt
    21841 actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt
    21901 gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tctatccgaa acgacgtatt
    21961 cccaccatta atatccactc gacgggcaag tccggtccgc tggcgcagta cattgtggaa
    22021 gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtgggtga tgcgacacca
    22081 caaccctctt ccgtgggccg tgactatgac accagtaaga aaatcaccga acaaagcgat
    22141 ggtttactgg cgaaggtggg aatgggcatg gggtccgccg ttgcggtgct gtttcaatct
    22201 ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc attcgtctcg
    22261 gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca cggtcttgct
    22321 ccgctggcga gccatccact gggtctggtc atgctggcgc tcatctgctc cttcccactg
    22381 ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt attgattggc
    22441 gtgcagattg gtctcggcaa tattccgccg catctggctt taccggcact gtttgccatc
    22501 aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt cgctggcgga agcccgtcag
    22561 gacacggttc gcgtcggtgt cccttctgta ctggtgagcc gctttttaac cggcgcaccg
    22621 actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct gaaacatgac
    22681 cgttatttat aagaccacca tcacccgtat cggcgcgagt gccactgacg ccctcagcga
    22741 ccagatgctc atcacctttc gtgaaggcgc gccagcggac ctcgaagagt attgcttcat
    22801 tcattgccac ggcgagttga aaggtgcact ccatcccggt ttgcaattta cgctcgggca
    22861 gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa gacaaccttc gcgaactggg
    22921 tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca ctgtccatgt
    22981 ggcaggccct gtacccgacg atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa
    23041 ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt gggcaaacct taggcgcgtt
    23101 cctgtgccac ggtctggttg ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga
    23161 caaagccgca aatgtggcac aagaaattaa cgccgaatat ggtgaaagta tggcgtacgg
    23221 ttttggtgct gacgccacta gcgagcaaag cgttctggcg ctctctcgtg gggtagatga
    23281 aatctttggt cgcgtggatt tgctggtcta tagcgccgga atagcaaaag cagcctttat
    23341 cagcgacttc cagctcggcg attttgaccg ttcgctacaa gtgaatctgg tgggttattt
    23401 cctgtgtgcc cgtgaatttt cgcgtttgat gatccgcgac gggattcagg gtcgcattat
    23461 tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc
    23521 gaaatttggt ggcgtcgggc tgactcaatc actggcgctg gatctggtgg agtacggcat
    23581 tacggtgcat tcactgatgc tcggtaacct gctgaaatcg ccgatgttcc agtcactgtt
    23641 gccacaatac gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt attacattga
    23701 caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg ctgaatatgc tgctgttcta
    23761 cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt
    23821 gatgttctga tcaacagcgg agatccatta aggatctccg tgagactata gaatgcctga
    23881 tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta
    23941 tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg
    24001 caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg ggttggcgtg
    24061 ggtcgttcca gcgggcgctt taaaccgcgg gtcgtggtcg ccatcggctg gacgatcagc
    24121 agcgcatcgt cgacaccttg tttatgaaag gactgaccgt cttcgcccga ccgcaaaaaa
    24181 ttcccgcaat taccggtatg catgcgggtg atttacagcc cgatgtgatc tttccccatg
    24241 atccactatc acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt
    24301 gtgaatgtta cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca
    24361 tgaaacctcg tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct
    24421 cggttgaaga attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg
    24481 tcattctgga acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag
    24541 aggaatccga tccgcctatc gatcataaaa cactcatcaa cacccacaag aaagagctga
    24601 ttgcagaagc tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca
    24661 gtaccgtttt gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca
    24721 acagcctgca tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc
    24781 caggcggaac gtttcgtaaa aaattggcct catttcacgg acagctggca gagaatgcct
    24841 tcgaacattt cagtttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg
    24901 gcgtaaccac cttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg
    24961 aagtgatttt gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca
    25021 gtcttgaaag cgtcgataag ctgattaccg acgcaggtat cgatccggcg tttcgtcagg
    25081 cgctggaaga gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta
    25141 ctgaacgcgg gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa
    25201 cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg
    25261 gtggtttcgg gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc
    25321 agtaccggca caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg
    25381 atgattgaaa gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg
    25441 gatctgatta ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa
    25501 ccgacgtcac cgctgggcct ggcggcaaaa gcggtgctgg atatctccgt agaacgcgaa
    25561 gcctgcccga tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac
    25621 gcgctggcga tggcggtcat gcaggcgcgc ggatttaatg aagaagattt tgcccgctcc
    25681 cacccagccg gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgccgt
    25741 gacgatgcca tcccacaggt ggcgttaacc gccagcgtga tggatgcgat gctggaactc
    25801 agccgcaccg gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc
    25861 tttaccgacg gcgatttacg tcgctggctg gtaggcggcg gcgcactcac cacgccagtt
    25921 aatgaagcga tgacgacagg cggcaccacg ttacaggcgc aaagtcgcgc catcgacgcc
    25981 aaagagatcc tgatgaagcg caaaatcact gccgcaccgg tggtggatga aaacggcaaa
    26041 ctcaccggcg caattaacct gcaggatttc tatcaggccg ggattattta atccttcatt
    26101 cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg tttccagcat ccgcgcgcag
    26161 gcagcccagt tatgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct
    26221 tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc
    26281 aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg
    26341 gtggctctcg ccagcactac cgcccgatga atagcatgtt ccagttcgcg cacgttcccc
    26401 ggaaaacgat agtgttgcag taaatttcgc gctccggcac ttaataccac gcgggagagc
    26461 cccagccgca aacgacactg ttcgcagaaa taccccgcca gcagaatgac atcatcgccc
    26521 cgctcacgca gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caagtcagcg
    26581 cgaaatcgcc ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc
    26641 acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc
    26701 ctcagcagct tggcctgcaa tgccaacgac aactcgccga tctcatccag aaacagagtg
    26761 ccgttatccg ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct
    26821 ttcacatgcc cgaacaactc actttccgcc acactttccg gcagtgcagc acagttgaga
    26881 tagaccagcg gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc
    26941 ttaccggttc ccgtctcacc gctgatcagg acgttgagat cggacgccgc cacaatctca
    27001 atctcttttt tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc
    27061 gcttcaaacg gcgtggcatc gcctggcatc atattctggc tttccagttg ttcaatcagc
    27121 aacgcattgc ttaacgctcc cgccgccagc gcagcaatca gccgtagctc ttcgtcgctg
    27181 aaaacatcga actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgc
    27241 ccggcaaaca atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc
    27301 aaaccgtcat agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg
    27361 cgggcaatcg cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacaccc
    27421 tttgccagac cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca
    27481 gacgcatcgc actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga
    27541 tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc
    27601 atctttgcct cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag
    27661 tcattttgac tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa
    27721 aaaactggca cgtaatctgc aattagcaag acatcttttt agaacacgct gaataaattg
    27781 aggttgctat gtctattgtg gtgaaaaata acattcattg ggttggtcaa cgtgactggg
    27841 aagtgcgtga ttttcacggt acggaatata aaacgctgcg cggcagcagt tacaacagct
    27901 acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg
    27961 aatttgtgca gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta
    28021 accatgcaga agaggaccac gctggggcgc tgaccgaact gatggcgcaa attcccgata
    28081 cgccgattta ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg
    28141 agtggaattt taatgtggtg aaaactggtg acacgctgga tatcggcaac ggcaaacagc
    28201 tcatttttgt cgaaacgcca atgctgcact ggccggacag catgatgact tacctgacag
    28261 gcgacgcggt gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt
    28321 tcaacgatga agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata
    28381 tcctgacgcc gttcagccgc ctggtaacac cgaaaattac cgagatcctg ggctttaact
    28441 taccagtcga tatgatagcg acctctcacg gcgtggtatg gcgcgataac ccgacgcaaa
    28501 ttgtcgagct gtacctgaaa tgggcggctg attatcagga agacagaatc accattttct
    28561 acgacaccat gtcgaataac acccgcatga tggctgacgc tatcgcccag gggattgcgg
    28621 aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa
    28681 tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg
    28741 tgatgatgcc gaaaatcgcc gggctggtgg aggagatgac tggtttacgc ttccgtaaca
    28801 aacgcgccag tgctttcggc tctcacggct ggagcggcgg tgcggtggat cgtctttcca
    28861 cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct gaaagcgaaa tggcgaccag
    28921 accaggacgc tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc
    28981 tcgcgccgct gccgcagagc acggtgaata aggtagtcaa agaagaaacc tctgccacca
    29041 cgacggctga cctcggccca cggatgcagt gcagcgtctg ccagtggatt tacgatccgg
    29101 caaaaggcga gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata
    29161 acttcctctg cccggaatgc tccctcggca aagacgtctt tgaagaactg gcatcggagg
    29221 caaaatgagt aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa
    29281 aaatattcgc aaacaggacg ccactattcc attaaccctg attgccgccg acagcatgga
    29341 tgagtacaac aaacctgacc tcagccatgt tatcagtcag gggcaacgtg ccgatgacct
    29401 tacccgccag acggcgggtg aatttgccga gcagtttaat ctgcacctgt ttccacaaac
    29461 ctgggtgacg gatatcgatg ccgaagcccg tgtggtgaaa agccagaata atcagtggca
    29521 atacgacaag ctagtactgg caaccggtgc cagtgccttt gtcccgcctg tgcctgggcg
    29581 tgagttaatg ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg
    29641 ggatgcccga cgcgtgttga ttgttggcgg tggtttgatt ggtagcgaac tggcgatgga
    29701 tttttgtcgt gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc
    29761 gttaatgcca ccggaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca
    29821 tctgctgttg aaatctcagt tacaggggct ggaaaaaacg gattctggca ttctggcaac
    29881 gctggaccac cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc
    29941 ggaaacagcc ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag
    30001 ttatctgcaa accagtaatg acgatattta tgcgctgggc gattacgcgg aaatcaacgg
    30061 tcaggtactg ccattcctgc agccgattca acttagcgcg atggtgctgg caaaaaatct
    30121 tctcggcaat aacacgccgc tgaaactacc gacgatgctg gtgaaaatca aaacgccgga
    30181 attaccgctg catctggcag gcgaaaccca gcgtcaggat ttacgctggc aaattaatac
    30241 cgaacgctag ggaatggtgg ctcgcggtgt tgacgatgct gaccagcttc gcgcctttgt
    30301 ggtcagtgag gatcggatga aagaggcatt tggattgttg aaaacattgc cgatgtaggt
    30361 gggctactgt gcctaaaatg tcggatgcga cgctagcgcg tcttatccga cctacgggga
    30421 cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac
    30481 acgttttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc
    30541 cccagagaca aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca
    30601 gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat
    30661 accagcgtag tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc
    30721 gccagcgcat catgaaacgc ccacgcgcgt tgattaacct gtgcctgcca gttcagccac
    30781 tgctgccaga aagcggcgag atccagttga ttgtccacca gcggcatcgt caccggatgc
    30841 gtcactccgt ggcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa
    30901 cttaacgtgg ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac
    30961 gatgacgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagcac gctccagttt
    31021 tgctgttgca cacttgctgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac
    31081 tgcgccagca ggtttcgcca cggctgcttc gctgccaaat cgccacccgg aagcgccact
    31141 gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc
    31201 cacaaagcgc cgttctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg
    31261 ccatccagcg gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt
    31321 accgtttgcg tcggcagatt catttcacgc gcccactggc tggagacata gcccggatgc
    31381 gcgtcatgca caacgtattt cggggtaaaa tcgtagatgt tttgcatcag gcgtaacgct
    31441 tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc
    31501 gcttgttcac cgcgcaccag gcagaaggta tttttcagat ccgctccgag acacagcaca
    31561 ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa
    31621 cggcgcagca tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg
    31681 cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg
    31741 ctgatagctg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt
    31801 tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg
    31861 tcaggggcga tatcatcaca aagctcagga acgtattttt tatccaccag cacaatcggc
    31921 gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac
    31981 atgaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc caccgcgtta
    32041 ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg
    32101 cccattttta actgtgcgat agccgcctgt aatgccgcct cttgttctgc atgttcacca
    32161 tgacttaccc attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag
    32221 cgacgatcga gcgggtcgcg gtactctttg tcacaagccg gacacaacgg aaacgccgcc
    32281 atcacggtaa acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg cgggccgcag
    32341 tgggtacagt tgataaacgg ataacgataa cgccgttcgc ctggggtatt catttcggca
    32401 aggcaagcag ggcaagtagc ggcatccggg acaatttgcg tattcatggc acctcccgcg
    32461 ctctggcgga tggtgaactc agtgggcagt tgtgaccaga tgtacggctc acgctcgacg
    32521 ctatcaatac gcgccagcgg cgggcagtgc tgatgcaatt gaacaagaaa cgtttccggg
    32581 tcttcccgca gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt
    32641 aattgctgtg ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga
    32701 atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga
    32761 attgcgtaac ccgcccagcc ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga
    32821 taccgcctgc tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc
    32881 tgtaagattt ttagaacatc agcgccgtac ggcggcgttt ttctgcactc agttgttcaa
    32941 gtttattacg atcgacacaa atcagcgcat gagtcgggca agccgccata cacgccgggc
    33001 cgtcttcacg atggttgcac aggtcacatt tattggcttc ggctttgtca gcccgtacat
    33061 tcagccccgc gccgctgttg cgaatcaccg gacgtaccac cacttccatc gcgccatacg
    33121 ggcaagccac aacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc
    33181 ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact
    33241 gacggcaaac tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa
    33301 atgtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca
    33361 cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca
    33421 aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat
    33481 ttactgttat ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta
    33541 gtgacgatga catgtgatga caatgtttat cgcgaaagag caatgagtga gtcgcggtgg
    33601 atcagttttc cgctgaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc
    33661 agccgtccaa taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc
    33721 ggaacgatat agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc
    33781 accgctacgc cgcgctcgtg caacgctttc atcgcaccta tcgccatatc gtcgttactg
    33841 gcaactaacg cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg
    33901 ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc
    33961 gccagcgcgt ctttataccc agcaagacgt tcaatgctgg tgggggaatc catcgagccg
    34021 gtaaggaaag caatctcctg atgtccggcg tttatcaact ctgccacggc attaaagctg
    34081 gtctgtttat gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc
    34141 accattatcg gctgactgtg cgcgtcaatg atgtcatcga tctcatccac gcttaaaaaa
    34201 cgcgggtaaa tcataatggc gttgcagcgc aaatccagca gatactgaat cgcctggcgc
    34261 tcttcttcgg cgctgtgttt accatctgcc aatagcaact gccgcccttt ctcttccgcc
    34321 attcgcgcgg catgaaagag taattcacta aaataaatgc cgtggtaaag cgtgttggtc
    34381 actaccagcc ccagcgtctg agtactcttc gccgacagat tgcgcgccag caagtttggg
    34441 cggtaaccgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag
    34501 ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact
    34561 tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc
    34621 gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa
    34681 tataaaaaat gaataattaa ctctcttgct tatttagtga caactattca tgattttgtg
    34741 aaaccggttt cctaattccg tttcagcatc ggcatttttc cgttacgtcg actgataaca
    34801 actacatcta ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg
    34861 gcacgctcgg agatagcggc actgggcggc gttgataaca tctcggcggt cacgcactgt
    34921 atgacgcgtt tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa
    34981 accatccccg gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt gattatcggc
    35041 aataccgttt cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc
    35101 gcacagcccg tgggtaaacc caaactcacg ccgcgtcgca ttggtgcggg gatcctcgat
    35161 gcgctgatcg gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa
    35221 ctgctggcga tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc
    35281 attctgaatg tgattggtga cggtgctttc ttcttcctgc cgctgatggt cgcggcatct
    35341 gccgacatca aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta
    35401 catccgagtt ttattgaact gatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc
    35461 ctgattccgg tcaccgcggt gaaatacacc tacacagtga tcccggcgct ggtgatgacc
    35521 tggtgcctgt catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac
    35581 ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc
    35641 ccgattggta tctggatcgg tagcgccatt tcggcgctgg tttacagcat tcatggttat
    35701 ctgggctggc tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg
    35761 atgcaccgcg tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaaggg
    35821 atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg
    35881 gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc
    35941 attatggcgg ggatttccga accggcgtta tacggcgtgg cgatccgcct gaaacgtccg
    36001 cttatcgcca gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt
    36061 gccagccact caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg
    36121 aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttt
    36181 atcctcacac tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg
    36241 cgaaagcatc agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga
    36301 aaatgtcagt atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg
    36361 aaggtgcgtt ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg
    36421 agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt
    36481 tttatcccag ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga
    36541 tggcagagat gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc
    36601 agggcgatga agtcacgccc aatcagcagg gcattgcttt ttatcgttct gtctttgaag
    36661 agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc
    36721 atctggtcac cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct
    36781 acgccagaac ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg
    36841 aaatcaacat tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg
    36901 aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag
    36961 ccgccaaaat cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg
    37021 gcaacttcta cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc
    37081 gggaaaacct gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc
    37141 gcgtatttcg cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga
    37201 aaaacaccgt cgattttgtc tctttcagct attacgcctc gcgctgcgcc tcggcggaga
    37261 tgaacgccaa caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc
    37321 aagtgagcga ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga
    37381 tgtacgatcg ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg
    37441 aacttgctgc caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata
    37501 tccgcgcaat gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg
    37561 gctgtattga tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct
    37621 acgtagaccg tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct
    37681 ggtggtataa aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg
    37741 atgcggcgtg aacgccttat ccggcctaca tgtcgcacga accaggaagg cccgataagc
    37801 gcagcgcatc gggcaattta gcatttggca tcagagctgg ttcaccccat caagaacatc
    37861 cctgtcctga ttccttaatg aaaaactact cttcttccac cgctaactgc gcgaagccgc
    37921 cattcccttc ccagccttcc agtcgttgat aaacggtttc taccgcatct ttaatcggct
    37981 gggtcatcgg gtagtaaaag ccgacgatat ccggctgtat gccgaggaaa atcacttcgc
    38041 caatatcttc tttcaactgg tcgataagat agttcagagg catgttatgg gtagtcatca
    38101 taaacatctc ggcgatatca tccgggtcga tgatgcggat ctcgccgggg tttagcccca
    38161 tatccgtggc gtcgacaatc agtaaccgcg tagggcgcag ttcacggatg gcgacgatgt
    38221 cgttttccgg tgcactaccg ccgtcaatca ccacccagtt acctttcggc gcggcggcgc
    38281 acttttccgc cagcagcgga cctgcgccat catcgcccat catgctattg ccaacacaga
    38341 gtaaaacgtc agtcacgcaa tctcctcacc atcaaataga tggcgttttc ctgatgaata
    38401 tcatgcagca tacttaacag cattgtactc cattcctgct gctgcgaagt ttgcgctgcc
    38461 cgggctttat cgaatgcgtt ggccagcatc ggcacatggt taatgtcgat gacgatctca
    38521 ccgtacttcg gtacgccttc cattttgcgg cgggcttcac tgcccgcctc cagagtggca
    38581 atccatgcca gatattcatc ccacgggcag gtgagcgccg cttccaggca atcgataacc
    38641 ccaaggtggt gaccaatcgc caggctgtaa tagaccacct gctgcgcctc ggcgggcgtg
    38701 gcatcgttct catcaataaa tttacggctc agttgactga acaccacctt ttcactcatc
    38761 ggatacgcac ctcttctaca acatgattca gatggctgac aatctcgttc agacgcggat
    38821 cgttttccgc ttccagccag cgtgcaacct gttcttcgcc ctgccctaac tgtgtcagga
    38881 agtcatcggc aatctgacga ccgtaacgat aacccgccag gcggcgtgct tcgcgatcca
    38941 ctttcacgcg cagcggctgc accatatcgc catgcaggat ctccgccggt tgttcatcca
    39001 gttcacccgg cccacgggcg tgaattttct gctccagcag gccgagcgcc attgcaaagc
    39061 cgtacagcgt ggcggcaggc gttggcgggc agccagggat ataaacatcc acagggacaa
    39121 ttttatccgt accgccccac acgcagtaga gatcgtggaa gatcccgccg ctgttaccgc
    39181 aggcaccgta ggagatacaa attttcgggt ccggcgcgga ctgccacgca cgcagcgcag
    39241 gggatcgcat tgcacgggtg accgcgccgg taaacagtaa aatatccgca tgacgcggtg
    39301 aaggaacgac tttaatgccg aagcgttctg catcaaacag cggcgataaa gtggcgaaaa
    39361 tttcgatttc gcagccgttg cagccgccgc agtccacgcg gtaaacatag gcagaacgtt
    39421 tgattttttt cagtaacgac gccttcatgc tggcgatgga ttcatccacc gtcatcggga
    39481 ccggaatgcc gttggcgtca cgggggccta ataaattgct catcagatgg cctctttcat
    39541 atggcgagtc agttcaatac ggtcggacgg caccaggcat ttctggcgct tacattccgg
    39601 gcaagtctca aagctttcgc ggtggttttc cgcgcggctg tcgccgttgt gcttaagcag
    39661 cgcaatggcg tagtcgatct ctttctggac ggcgaaagga cgattgcaga cgcgacagtt
    39721 gcacagcgcg aagcgagact gttgcaggaa gtcttctttc ttccacaccg ccagttcgta
    39781 ctcttgcgac agcttaatcg ccgccgtcgg gcaaacttct tcgcagcgtc cacagaagat
    39841 gcagcgcccg agattaaact cccaggccag ctctcctgtg gcgaggtcag tttcaaccgt
    39901 taaggcgttt gacgggcagg cattgacgca ggccgcgcag ccgatgcact gctgcgggtt
    39961 ctgctctggc ttaccacgga agtttttatc aaccgcaatc ggctccagcg gataagacga
    40021 ggtcgccgta ccggttttga taactttttt gataaaggta aacatggcga ttccttattt
    40081 cagcggcgag tttttacgct caatgctgta gcgctcgagt tctttgtacg gcaccacttt
    40141 gctcttcttc ttacgtacat cgaccacagt catgcggtcg gtacaggagt agcaagggtc
    40201 caggctaccg ataatcagcg gcgcatcgga aacagtgttg ccacgcagca tgtagcgcag
    40261 ggtcggccag ttcgcgtagg tcgcggcacg gcagcgccag cggtacagct tctggttgtc
    40321 gccagtcatg ctccagtgga tatcatcacc gcgcggcgct tcggcaaagc ccagcgcgaa
    40381 acggtgcgga atgtaggtaa agccttccac catcagcggg ccacccggca ggttatccag
    40441 accgtagtcg atcatgttca gcgcggtata gacttcgttg atacgcactt tcagacgcga
    40501 aataacgtcg cagccctgct cgctgtggac ttccattggc agcaggccat aaccaacaaa
    40561 cgggtgatca gcgcgggtat cgcgagcgtg tccgctggcg cggaccatcg ggccaacgtt
    40621 actgaaatcg cgggcaattt ccgggtccag acgaccaatg ccgacggtgc gctgttccat
    40681 gttcggtgtg ctcagcagca catccaccag ctcctgcact tcacgacgca tctgttgcgc
    40741 cagctggcgg gtctggatca tgtcatcttt caacagatcg cgacgaatcc cgccgatcag
    40801 gttcaggccg taggttttac gcgcaccggt gaggatctcg gccattttca tggaggtttc
    40861 acgcacgcgg aagaattgca taaagccgga gtcgaagccg gtaaagtggc aggcgaggcc
    40921 gaggttgagc agatgcgagt gcaggcgttc cacctccagc agaatggcgc ggatcatctg
    40981 cgcacgttct ggcacctgaa tacccatcgc gttttccacc gacgtggtgt aggcggtgct
    41041 gtgggcgaag ccgcagatcc cgcacacacg gtcagagagg aaggtcactt cgttataacc
    41101 catgcgggtt tccgccagtt tttccatacc gcgatggaca tagaacaagc ggtagtctgc
    41161 gtcgataatg ttttcgccat cgacgaacag acggaagtgg cccggttcgt cagaagtgac
    41221 gtgcagcgga ccaatcggca cgacgttgtt tttcttgtcg cccagttcgt tgatgaactc
    41281 gtaggtttca gcatcggtgg tcggtgccgg acgctgacga taatccatgc tgtctttacg
    41341 cagcggataa agttcatccg gccagtcatc cggcagcacc agacggcgtt catccggcag
    41401 accaaccgga atcaaaccgt acatatcgcg cacttcgcgc tcgccccaca ccgccgccgg
    41461 aacgcgcggc gtcacggacg gatactccgg tttgttggca tcaacttcga cgcgaaccgt
    41521 tacccagcac ttagtcccct tctccatcga cagcacgtaa taaacggcgt aatgaccatt
    41581 cagtttgcgt tcgtcgttac caaaaagcac cgacagccag cccccctgct tgtagtagag
    41641 aaactccacc acttccggca gatagttcac ctttacggtg acagtcagct gatctttggt
    41701 ctgccaggcg tggtccagca cgacgcccgg aaatgcctca ttcagcgcgg cgagataatg
    41761 ttgacctaat ttttcttcag acatgctcaa actctcttta atcacgccgc cagcaaggag
    41821 acgaacgcta aaaatgcaaa gccaaaccca gcccaggtaa tgcgcggagt aatatcaaga
    41881 cgcagacggg ccatgctgtt ttcgaacagc gcgataacca ggacgccgac caccagtttt
    41941 acgatcgcaa tcaccagcgc cagcagcagt ccaccggcgg tgaaggtttc catttgtccc
    42001 cacggaataa acaccccgac gaacatctgc aacaccacca gctgtttcag gctgataccc
    42061 catttcatga cgccaaagcc gctgccgcta tactcagaga gcggaccttc ctgcaactcc
    42121 tgctcggctt ccgccaggtc gaacggcagt ttgcccattt cgataaaagt ggcgaacgca
    42181 caggcgcaga gcgccagcac cagcgggatg ctttggctca gcggccagtg ataaacggtg
    42241 tcggtaatgt tgctgatgtt ggtggaaccg gcaacctgtg cggcaaccca cagaccaagc
    42301 agcaacatcg gttcgaccag cacgccaagc atcgcttcac ggctcgcacc gatagcggta
    42361 aacgggctac cggtatccag accggaaatg gcaaagaaga aacgcgcgat ggcgaagaga
    42421 tacagtaagg tgatcaaatc gcccagttgc ggcagcggag aaccgacggt caccaccggc
    42481 agcgcagtag cgatagtcag catgacgccc accatcacat acggcgtcag gcggaacacc
    42541 cagccagagg catccgggcc aacgctctgg cggcccagca gtttgataat gtcgcgatac
    42601 tcctgcaaca cgcccggccc gcgacggtta tgcagacggg cgcgcgccac gcgggtaata
    42661 ccggagagca gcggcgcaac ggcaaataac accagcgcct gaattaacgg atataaaaca
    42721 ctcattctca ggctcctcgt gaaacaataa tcaccaccag taccgccagt tcaaccagcg
    42781 ccatccggcg gaacagcaac gcactacctt cgcactgcca gcccggcacc agcgacaccg
    42841 gattcagcca tttgcgcagt ttcagcactg gcgcaaacgc ctgtttaacc ggcatggcaa
    42901 aaccgtgagc ggtaatcacc attgattttt catggtcgta gccgcacacc caggccgcac
    42961 cgcgggaacg tgacggcagg cggtcgcctt tgcaaatcgc cataatgatg aatggcagca
    43021 gcgggcaggc aatcagcagc aatgtgatca ttggttgaga aacggtggtg ttagcaggct
    43081 ccagaggcag aggtacggca gcagagagca tcggcagcag ccacggcgca gcaacaccgc
    43141 caattacgca gcaaatcgcc agtgccacta cgcttacgct catcaggagc ggcgcacagg
    43201 tggcgttttc ggcttctttg gtacgcggcg cgccgaggaa cgtgacgcca tagactttcg
    43261 ccatacacat caccgccagc gcaccggtaa ttgccagacc cacagcgagc agcggcccca
    43321 gcagacgggc aacaaacgcg ccactattgc tcagtttgaa aaaggattga tagataaccc
    43381 attccccggc aaacccgttc agcggcggca gcgcagccat tgccatcaac ccgactaaca
    43441 tggcgatgga gataaccggc attttcttgc caataccacc gagtttttcg atatcgcgat
    43501 gaccggtacg gaaccagacg ctccccgccc cgaggaacag tacgcttttg aacaggctat
    43561 ggttaagcag atggtacaga ccaccgacca ggccaagagc aatcagcgcc ggttgttcga
    43621 gcgcgatacc cgttacgcca gcgcccagcc ccagcaggat aatgccgata ttttccaggg
    43681 tgtggtaagc cagcaggcgc tggatgttgt gctccatcag cgcatacaga ccaccgacaa
    43741 acgcggtgat catgccgagc accagcagca cgatccccca ccacagcggt gcattaccgc
    43801 ccagcagtga cagggttaaa atgcccagca ggccaatttt catgactacc gtagaaaaca
    43861 acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg tgcagcggaa
    43921 taatcccggc gagcaggcca aagccaatca caccgagcag ccagatatcg gaaccgagcg
    43981 gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa cgctgccaca
    44041 gcagccagca agcaatcgcc agcagcagag tgccaagacg ccccagcgca aaccacagtt
    44101 tgccctcttt tgtttcgacg taatactcga tggtgtggct gttaaagcca tccgccaggg
    44161 tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag ttctttgtcc ggcgcgcagc
    44221 tcatggcgtg atgcaggcgc gctttcgata acgtgatccc ctgaaccagc gaattgcagt
    44281 agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc gatgtaatcg gctttctcgc
    44341 ttatttccca aatagtcatt gtcaggttac ccgtttaaca gagatgccag ctttaagcat
    44401 tttctgtgcc aatttttaat ttattgttat taaaacgatt tttaagctaa agatgaattt
    44461 cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt attcaccgca gggataatca
    44521 acactggcac aattattgct tgtagctggc aatagttaat gggaggcgat atgcacgaaa
    44581 taaccctctg ccaacgggca ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa
    44641 aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc
    44701 ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc
    44761 tcgaagaaca agaggtcgaa tgctggtgtg aaacatgcca acagtatgtg acgctattga
    44821 cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat gctacagatt gtggcagacg
    44881 acggtttaca gattcggcgg atagaaatag accaggagtg agcgatgtgt acaacatgcg
    44941 gttgcggtga aggcaacctg tatatcgagg gtgatgaaca taaccctcat tccgcgtttc
    45001 gtagcgcgcc atttgccccg gcggcacgcc cgaagatgaa aatcaccggc attaaagcgc
    45061 ctgaatttac ccccagccag actgaagaag gcgacctgca ttacggtcat ggcgaagcgg
    45121 gcactcacgc accgggcatg agccagcgtc ggatgctgga agtcgaaatt gacgtgctgg
    45181 acaaaaacaa ccgtctggct gaacgcaacc gcgcgcgctt tgctgcccgc aagcaactgg
    45241 tgctcaacct ggtttccagc ccaggttccg gtaaaaccac cctgctgacg gaaaccctaa
    45301 tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg tgaccagcaa accgtgaacg
    45361 atgccgcacg cattcgcgct accggcacac cagcgattca ggtgaacaca ggtaaaggct
    45421 gccatcttga cgcacagatg attgccgacg ccgcaccgcg tctgccactg gacgataacg
    45481 gtattctgtt tatcgaaaac gttggcaacc tcgtatgccc ggccagcttc gatctcggtg
    45541 aaaaacacaa agtggcggtg ctttccgtta ccgaaggtga agacaaaccg ctgaaatatc
    45601 cgcatatgtt tgccgccgcc tcgctgatgc tgctcaacaa agttgacctg ttgccgtatc
    45661 tcaactttga cgttgagaag tgcatcgcct gcgcccgcga agtcaatcca gaaattgaaa
    45721 tcatccttat ttccgccacc agcggtgaag ggatggacca gtggctgaac tggctggaga
    45781 cacagcgatg tgcataggcg ttcccggcca gatccgcacc attgacggca accaggcaaa
    45841 agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg ttagtcggca gctgcgatga
    45901 aaacggtcag ccgcgcgtgg gccagtgggt actggtacac gttggctttg ccatgagcgt
    45961 aattactgaa gccgaagcac gcgacactct cgacgcctta caaaacatgt ttgacgttga
    46021 gccggatgtc ggcgcgctgt tgtatggcga ggaaaaataa tgcgttttgt tgatgaatat
    46081 cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc gcgaacgtgc ttcacatctc
    46141 tcttacaccg ccgaacgccc tctgcggatt atggaagtgt gcggtggtca tacccacgct
    46201 atttttaaat tcggcctcga ccagttactg cctgaaaacg ttgagtttat ccacggtccg
    46261 ggttgccctg tgtgcgtact gccgatgggt agaatcgaca cctgcgtgga gattgccagc
    46321 catccggaag tcatcttctg tacctttggc gatgccatgc gcgtaccagg gaaacaggga
    46381 tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca tcgtttactc gccgatggat
    46441 gcgttgaaac tggcgcagga gaatccaacc cgcaaagtgg tgttcttcgg cttaggtttt
    46501 gaaaccacca tgccgaccac cgccatcact ctgcaacagg cgaaagcgcg tgatgtgcag
    46561 aatttttact tcttctgcca gcacattacg cttatcccga cgctgcgcag tttgctggaa
    46621 cagccggata acggtatcga cgcgttcctc gcgccgggcc acgttagtat ggttatcggc
    46681 actgatgcct ataattttat cgccagcgat tttcatcgtc cgctggtggt cgctggtttc
    46741 gaaccccttg atctactgca aggcgtggtg atgctggtgg agcagaaaat agcggcccac
    46801 agcaaggtag agaatcagta tcgtcgggtg gtaccggatg ccggtaacct gctggcgcaa
    46861 caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagcg aatggcgcgg cttaggcgtg
    46921 attgaatctt ctggcgtgca cctgacgccg gattatcaac gattcgatgc cgaagcacat
    46981 ttccgcccgg caccgcagca ggtctgcgat gacccgcgcg cgcgttgtgg tgaggtatta
    47041 acgggcaaat gtaagccgca tcaatgcccg ctgtttggta acacctgtaa tcctcaaacc
    47101 gcatttggtg cactgatggt ctcctccgaa ggagcgtgcg ccgcgtggta tcagtatcgt
    47161 cagcaggaga gtgaagcgtg aataatatcc aactcgccca cggcagcggt ggccaggcga
    47221 tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc caacccgtgg ctggcagagc
    47281 aggaagacca ggcacgtctt gatctggcgc agctggtagc agaaggcgat cgtctggcgt
    47341 tctccaccga cagttacgtc attgacccgc tgttctttcc tggcggtaat atcggcaagc
    47401 tggcgatttg cggcaccgcg aatgacgttg cggtcagtgg cgctattccg tgctatctct
    47461 cctgtggctt tatcctcgaa gaaggattgc cgatggagac actgaaagcc gtagtgacca
    47521 gcatggcaga aaccgcccgc acggcaggca ttgccatcgt tactggcgat actaaagtgg
    47581 tgcagcgcgg cgcggcagat aaactgttta tcaacaccgc tggcatgggc gcaattccgg
    47641 cgaatattca ctggggcgca cagacgctaa ccgcaggcga tgtattgctg gttagcggta
    47701 cactcggcga ccacggggcg actatcctta acctgcgtga acaactgggg ctggatggtg
    47761 aactggtcag cgactgcgcg gtgctgacgc cgcttattca gacgctgcgt gacattcccg
    47821 gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa cgcggtggtt catgagttcg
    47881 cggcagcctg cggttgtggt attgaaattt ctgaatcagc actgccggtt aaacctgctg
    47941 tgcgcggcgt ttgcgaactg ctgggactgg acgcccttaa ctttgccaac gaaggcaaac
    48001 tagtaatagc tgttgaacgc aacgcagcag agcaagtgct ggcagcgtta cattcccatc
    48061 cactggggaa agacgcggcg ctaattggtg aagtggtgga acgtaaaggt gttcgtcttg
    48121 ccggtctgta tggtgtgaaa cgaaccctcg atttaccaca cgccgaaccg cttccgcgta
    48181 tatgctaata aaattctaaa tctcctatag ttagtcaatg accttttgca ccgctttgcg
    48241 gtgctttcct ggaagaacaa aatgtcatat acaccgatga gtgatctcgg acagcaaggg
    48301 ttgttcgaca tcactcggac actattgcag cagcccgatc tggcctcgct gtgtgaggct
    48361 ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg cggctattgt gttgtggcaa
    48421 gcgcagactc aacgtgcgtc ttattacgca tcgcgtgaaa aagacacccc cattaaatat
    48481 gaagacgaaa ctgttctggc acacggtccg gtacgccgca ttttgtcgcg ccctgatacg
    48541 ctgcattgca gttacgaaga attttgtgaa acctggccgc agctggtcgc tggtgggcta
    48601 taccccaaat ttggtcacta ttgcctgatg ccactggcgg cggaagggca tatttttggt
    48661 ggctgtgaat ttattcgtta tgacgatcgt ccctggagcg aaaaagagtt caatcgtctg
    48721 caaacattta cgcagatcgt ttctgtcgtc accgaacaaa tccagagccg cgtcgtcaac
    48781 aatgtcgact atgagttgtt atgccgggag cgcgataact tccgcatcct ggttgccatt
    48841 accaacgcgg tgctttcccg cctggatatg gacgaactgg tcagcgaagt cgccaaagaa
    48901 atccattact atttcgacat tgacgatatc agtatcgtct tacgcagcca ccgtaaaaac
    48961 aaactcaaca tctactccac tcactatctt gataaacaac atcccgccca cgaacagagc
    49021 gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg
    49081 atcaatctcc acgagcggga cgatttagcc ccctatgaac gcatgttgtt cgacacctgg
    49141 ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt ctggcgacac catgctgggc
    49201 gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca ctaccaatct gaatttgctg
    49261 cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc
    49321 catcgtctga aagaacggct ggttgatgaa aacctcgccc tgaccgagca gctcaacaat
    49381 gttgatagtg aatttggcga gattattggc cgcagcgaag ccatgtacag cgtgcttaaa
    49441 caagttgaaa tggtggcgca aagtgacagt accgtgctga tcctcggtga aactggcacg
    49501 ggtaaagagc tgattgcccg cgcgatccat aatctcagtg ggcgtaataa tcgccgcatg
    49561 gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg aaagcgatct gtttggtcat
    49621 gagcgtgggg cttttaccgg tgccagcgcc cagcgcattg gtcgttttga actggcggat
    49681 aaaagctccc tgttcctcga cgaagtgggc gatatgccac tggagttaca gccgaagttg
    49741 ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca gcaacaaaat cattcagacg
    49801 gacgtacgtt taattgccgc gactaaccgc gatctgaaaa aaatggtcgc cgaccgagag
    49861 ttccgtagcg atctctatta ccgcctgaac gtattcccga ttcacctgcc gccactacgc
    49921 gagcgtccgg aagatattcc gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt
    49981 ctggggcgca atatcgacag cattccagcc gagacgctgc gcaccttgag caacatggag
    50041 tggccgggta acgtacgcga actggaaaac gtcattgagc gcgcagtatt gctaacacgc
    50101 ggcaacgtgc tgcagctgtc attgccagat attgctttac cggagcctga aacgccgcct
    50161 gccgcaacgg ttgtcgctca ggagggcgaa gatgaatatc agttgattgt tcgcgtgctg
    50221 aaagaaacca acggcgtggt tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa
    50281 cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg ataaatcggc attgatttaa
    50341 ctgcaaattg ccggacagat ctgcctgtcc ggcatactat tcacgaggtt ttttcggacg
    50401 atatttttcc ggcagttctg gcaccggacg cttgtcatcg atgagatgac gcacggttaa
    50461 gatcggatga cgccacagca ttctcggccc ggcccaacgc ataatctgtt tcatctcttc
    50521 acgctttgca ggctggtaac agtgcaccgg acactgctta caggctggtt tctcttcgcc
    50581 gaacacacat ttatccagcc gcttttgcgc gtaaacaaac aacgcctcgt aatgctccgg
    50641 ctccgctgac gcctgcgggc atttcgcttg ataaagatcg atcatttttt taatcgtcag
    50701 tttttcacga gagatacgct tgccggacat gctgcctcca tactcttaag gtgtatttat
    50761 attacaactt aatttgaaag gggactatgg ctctgaagga ggaggtttat tcaaccgacg
    50821 caattttgcg tacttgcttc ataagcatca cgcaaaagct gcaaaacagc atctttcccg
    50881 gaaccagcat caagaactcg ccgttcgctt cttcccctga aatgattaac tccggtatca
    50941 tgtgcgcctt atgtgattac aacgaaaata aaaaccatca caccccattt aatatcaggg
    51001 aaccggacat aaccccatga gtgcaataga aaatttcgac gcccatacgc ccatgatgca
    51061 gcagtatctc aggctgaaag cccagcatcc cgagatcctg ctgttttacc ggatgggtga
    51121 tttttatgaa ctgttttatg acgacgcaaa acgcgcgtcg caactgctgg atatttcact
    51181 gaccaaacgc ggtgcttcgg cgggagagcc gatcccgatg gcggggattc cctaccatgc
    51241 ggtggaaaac tatctcgcca aactggtgaa tcagggagag tccgttgcca tctgcgaaca
    51301 aattggcgat ccggcgacca gcaaaggtcc ggttgagcgc aaagttgtgc gtatcgttac
    51361 gccaggcacc atcagcgatg aagccctgtt acaggagcgt caggacaacc tgctggcggc
    51421 tatctggcag gacagcaaag gtttcggcta cgcgacgctg gatatcagct ccgggcgttt
    51481 tcgcctgagc gaaccggccg accgcgaaac gatggcggca gagctgcaac gcactaatcc
    51541 ggcggaactg ctgtatgcag aagatttcgc cgagatgtcg ctgattgaag gccgtcgcgg
    51601 cctgcgccgt cgcccgctgt gggagtttga aatcgacacc gcgcgccagc agttgaatct
    51661 gcaatttggc acccgcgatc tggtcggttt tggcgtcgag aacgcgccgc gcggactttg
    51721 tgctgccggt tgtctgttgc agtatgcgaa agatacccaa cgcacgaccc tgccgcatat
    51781 tcgttctatc accatggaac gtgagcagga cagcatcatt atggatgccg cgacacgtcg
    51841 taacctggaa attactcaga acctggcggg cggtgcggaa aatacgctgg cttctgtgct
    51901 cgactgcacc gtcacgccga tgggtagtcg tatgctgaaa cgctggctgc atatgccagt
    51961 gcgcgatacc cgcgtgttgc ttgagcgcca gcaaactatt ggcgcattgc aggatttcac
    52021 cgccgggcta cagccggtac tgcgtcaggt cggcgacctt gaacgtattc tggcacgtct
    52081 ggctttacga actgctcgcc cacgcgatct ggcccgtatg cgccacgctt tccagcaact
    52141 gccggagctg cgtgcacagt tagaaactgt cgatagtgca ccggtacagg cgctacgtga
    52201 gaagatgggc gagtttgccg agctgcgcga tctgctggag cgagcaatca tcgacacacc
    52261 gccggtgctg gtacgcgacg gtggtgttat cgcatcgggc tataacgaag agctggatga
    52321 gtggcgcgcg ctggctgacg gcgcgaccga ttatctggag cgtctggaag tccgcgagcg
    52381 tgaacgtacc ggcctggaca cgctgaaagt tggctttaat gcggtgcacg gctactacat
    52441 tcaaatcagc cgtgggcaaa gccatctggc acccatcaac tacatgcgtc gccagacgct
    52501 gaaaaacgcc gagcgctaca tcattccaga gctaaaagag tacgaagata aagttctcac
    52561 ctcaaaaggc aaagcactgg cactggaaaa acagctttat gaagagctgt tcgacctgct
    52621 gttgccgcat ctggaagcgt tgcaacagag cgcgagcgcg ctggcggaac tcgacgtgct
    52681 ggttaacctg gcggaacggg cctataccct gaactacacc tgcccgacct tcattgataa
    52741 accgggcatt cgcattaccg aaggtcgcca tccggtagtt gaacaagtac tgaatgagcc
    52801 atttatcgct aacccgctga atctgtcgcc gcagcgccgt atgttgatta ttaccggccc
    52861 gaacatgggc ggtaaaagta cctatatgcg ccagaccgca ctgattgcgc tgatggccta
    52921 cattggcagc tatgtaccgg cacaaaaagt cgagattgga cctatcgatc gcatctttac
    52981 ccgcgtaggc gcggcagatg acctggcttc cgggcgctca acctttatgg tggagatgac
    53041 tgaaaccgcc aatattttac ataacgccac cgaatacagt ctggtgttaa tggatgagat
    53101 cgggcgtgga acgtccacct acgatggtct gtcgctggcg tgggcgtgcg cagaaaatct
    53161 ggcgaataag attaaggcat tgacgctgtt tgccacccac tatttcgagc tgacccagct
    53221 accggagaaa atggaagacg tcgccaacgt gcatctcgat gcgctggagc acggagacac
    53281 cattgccttt atgcatagcg tgcaggatgg cgcggcgagc aaaagctacg gcctggcggt
    53341 tgcagctctg gcaggcgtgc caaaagaggt gattaagcgc gcacggcaaa aactgcgtga
    53401 gctggaaagc atttcgccga acgccgccgc tacgcaagtg gatggtacgc aaatgtcttt
    53461 gctgtcagta ccggaagaaa cctcgccagc ggtcgaagct ctggaaaacc tggacccgga
    53521 ttcactgact ccgcgtcagg cgctggaatg gatttatcgc ttgaagagcc tggtgtaata
    53581 acaattcccg atagtctttt gctatcggga atattaacga caactgacga ataaaataaa
    53641 aaccccctgt ataataggaa agcttatttt acagggtaaa accatgccat ctacacgcta
    53701 tcaaaaaatt aatgcccatc actatcgcca tatatgggtc gttggtgata tacatggtga
    53761 atatcaatta ttacaatccc gcttacatca actctctttt tgccctgaaa ccgacttact
    53821 tatttctgtc ggcgataata ttgatcgtgg gccggagagt cttgacgtcc tgcgcctgct
    53881 aaaccagccc tggtttatct ccgttaaagg caaccacgaa gcaatggagc tggatgcgtt
    53941 cgcgaccggc gatggcaata tgtggcttgc cagcggtggt gactggtttt tcgatttaaa
    54001 tgattcagag caacaagagg caatagatct gttgctgaaa ttccatcacc ttccacatat
    54061 tattgaaatc actaacgaca taataaaata tgtcatcgca catgcagatt atccgggtga
    54121 tgaatatctc tttggcaaag aaatagcgga gagcgaatta ctctggcctg tttatcgtgt
    54181 gcagaaatcg cttaatggcg agttacaaca aataaacggc gctgattatt ttatatttgg
    54241 acatatgatg tttgataaca ttcagacgtt cgctaaccag atttatattg ataccggatc
    54301 gccgaaaagt gggcggctgt cattttataa aataaggtag tctcatgctt cttctgtgaa
    54361 gcatgagtaa ccagatgtta ttgcaggcca ttattcattt ttcgctacca gcaaagagag
    54421 atcctgcttc gccagcgcgc gactggcact ctccggcaaa ccgtcgtctg taataatctg
    54481 atcaaactcg cttaatggta acgccagcca tgtcgccacc tgaccatatt tcgtcgcatc
    54541 acagaccaaa actcgctggc ggctggcact agcaatcgcc cgtttcaccg tgactttatc
    54601 ttccgctggc gtagaaagcc cccgcacact ccatgacgat gcagaaataa aagcctgatc
    54661 aatcatcagg ctgcgcagca tggtcgcagc ggcttccccg acacaggaac ggttttcccg
    54721 acacactgca ccgccagtgt gaataattgt gcaattactg ttgtcgagca agtagttcgc
    54781 aataacgaaa tcgtttgtga ccacagtcag tgactccatg tgaatcagat gctgtgctat
    54841 cgctaacgtg gtcgttcctg catccagata gatacaactt cccggctgaa caagacttgc
    54901 cgccagcttg ccaatagccg ctttttgcgt cattgctagc gcagttttta cctgatgaga
    54961 aggttcatgc gccacgcgtc ccggagactg gacgcctccg gacaccagca caacggctcc
    55021 ctgctgctcc agtttttgta aatcccgacg aatggtcata tgtgatacat tcattctgtc
    55081 cgttagttca gcaatactga caatgccttt ttcagctacc atctcaagga tgatttggcg
    55141 acgctctacg gggatcaact tttgctcctt cctttgtcct gctgacattc tacgctattt
    55201 gcctgcgaaa cgtgcgcggc gcaactaacg cttagttcac ataaaataac acacaatgtt
    55261 aatttatgtg aatcggctca ccatatcgtt atcttccagc gcttatattc acaatatcaa
    55321 acaaaatatc acttaaatta acaaggagag cagatgaaaa cgggatctga gtttcatgtc
    55381 ggtatcgttg gcttagggtc aatgggaatg ggagcagcac tgtcatgtgt ccgcgcaggt
    55441 ctttctacct ggggcgcaga cctgaacagc aatgcctgcg ctacgttgaa agaggcaggt
    55501 gcttgcgggg tttctgataa cgccgcgacg tttgccgaaa aactggacgc tctgctggtg
    55561 ctggtggtca atgcggccca ggttaaacag gtgctgtttg gtgaaacagg cgttgcacaa
    55621 catctgaaac ccggtacggc agtaatggtt tcttccacta tcgctagtgc tgatgcgcaa
    55681 gaaattgcta ccgctctggc tggattcgat ctggaaatgc tggatgcgcc agtttctggt
    55741 gatgcagtaa aagccgctaa cggtgaaatg actgtcatgg cctccggtag cgatattgcc
    55801 tttgaacgac tggcacccgt gctggaagcc gttgccggaa aagtttatcg cataggtgca
    55861 gaaccgggac taggttcgac cgtaaaaatt attcaccagt tgttagcggg cgtacatatt
    55921 gctgccggag ccgaagcgat ggcacttgca gcccgtgcgg ggatcccgct ggatgtgatg
    55981 tatgacgtcg tgaccaatgc cgccggaaat tcctggatgt tcgaaaaccg gatgcgtcat
    56041 gtggtggatg gcgattacac cccgcattca gccgtcgata tttttgttaa ggatcttggt
    56101 ctggttgccg atacagccaa agccctgcac ttcccgctgc cattggcctc aacagcattg
    56161 aatatgttca ccagcgccag taacgcaggt tacgggaaag aagacgatag cgcagttatc
    56221 aagattttct ctggcatcac tctaccggga gcgaaatcat gatcaagatt ggcgttatcg
    56281 ccgatgattt taccggcgcg acggatatcg ccagttttct ggtggaaaac ggtctaccaa
    56341 cggtacaaat taacggtgtt ccaacaggta aaatgccgga agcaatcgac gcactggtga
    56401 tcagcctgaa aacgcgctcc tgtccggtgg ttgaagccac acagcaatcg ctggcggctc
    56461 tgagctggtt gcaacagcaa ggttgcaaac agatctattt caaatactgc tctactttcg
    56521 acagtacggc gaaaggtaat atcggcccgg ttaccgatgc attaatggat gctctcgaca
    56581 cgccgtttac ggtcttctct ccggccctgt cggtcaacgg acgtacggtt tatcaggggt
    56641 atttgttcgt aatgaatcaa ctgctggccg aatccgggat gcgccatcac ccggtaaatc
    56701 ccatgaccga cagctatctt ccccgtctgg ttgaagcgca atccacaggg cgctgcggcg
    56761 tcgtttcggc acatgttttc gaacaaggtg tggatgccgt tcgtcaagag caggctcgct
    56821 tacagcaaga gggctaccgc tacgcggtgc ttgatgcgct gaccgaacac catctggaaa
    56881 ttcagggaga agccttgcgc gatgccccac tggtaacggg cggttctggt ctggcgattg
    56941 gcctggcccg gcagtgggcg caagaaaacg gtaaccaggc tcgcgaagca gggcatccgc
    57001 tcgctgggcg cggcgtagtg ctctccggtt catgctctca aatgaccaac cgccaggtgg
    57061 cacattaccg tcaaattgca ccagcccgtg aagttgatgt ggcacgctgc ctctcaaccg
    57121 aaactctggc cgcttatgca cacgaactgg cagagtgggt tctgggccag gaaagtgtac
    57181 ttgctccact ggtttttgcc accgccagca ctgacgcatt ggcagcaatt caacagcaat
    57241 acggtgcaca aaaagccagt caggcagtag aaactctgtt ttctcaacta gcggcgcggt
    57301 tagcagcgga aggcgtgaca cgctttattg tcgcaggcgg tgagacctcc ggcgtagtca
    57361 cacagagcct gggcataaaa gggtttcata ttggcccaac catttccccc ggcgtgccgt
    57421 gggtaaacgc actggataag cctgtctcac tcgcccttaa atctggcaac ttcggtgatg
    57481 aagccttttt tcacgagccc aaagagagtt tttatcatga gcgatttcgc aaaagtagag
    57541 cagtctttgc gagaggagat gacgcggatt gccagttcat tctttcagcg cggctatgca
    57601 accggttcgg ctggcaatct gtcgctgctt ttacctgacg ggaatttact ggcgacaccg
    57661 acaggttcat gcctgggcaa tctcgatccg cagcggcttt ccaaagtcgc cgcggatggc
    57721 gaatggttaa gtggtgacaa accctcgaaa gaggtgctct ttcatctggc gctgtatcgc
    57781 aacaatccgc gctgtaaagc ggtggtgcat ttgcacagca catggtcgac ggcgctttcc
    57841 tgcctgcaag ggctggacag cagcaacgtt attcgtccgt tcacaccata cgtggtgatg
    57901 cggatgggaa atgtcccgct ggtgccctat taccgaccgg gcgataaacg catcgcacag
    57961 gatctggcgg aactggcagc agacaatcag gcttttttac tggcaaatca tggcccagtg
    58021 gtttgcggtg aaagcctgca agaagccgcc aacaatatgg aagagctgga ggaaacggca
    58081 aagctgattt ttattctcgg tgaccgcccg atccgtatct gaccgcaggt gaaattgcgg
    58141 aattaaggag ttaatgcaat gcctcgtttt gcagctaatt tatccatgat gttcaccgaa
    58201 gtgcctttta ttgaacgctt cgccgcagcg cgaaaagccg ggttcgatgc tatggaattt
    58261 ctgtttccct atgactactc caccctgcaa atccaaaagc aactggagca aaaccatctg
    58321 acactggcgc tgttcaatac cgctcccgga gatattaatg cgggggaatg gggattatcc
    58381 gcccttcccg gacgtgagca cgaagcacac gcagacatcg acctggcact ggaatatgca
    58441 ttggcgctca attgtgaaca agtccatgtg atggcaggcg tcgtgcccgc tggcgaagat
    58501 gctgaacggt accgggcagt atttatcgat aatctccgct acgctgccga ccgctttgcg
    58561 ccacacggca agcgaatatt agttgaagca ctcagccccg acgttaagcc tcattatctc
    58621 ttctccagcc agtatcaggc actggctatt gttgaagagg ttgcgcgaga taacgtgttt
    58681 attcaactag atacttttca tgcgcaaaaa gtagatggca acctgacgca tttaatccgc
    58741 gactacgctg ggaaatatgc gcacgtacaa attgccggac tacctgatcg acatgaaccg
    58801 gacgatggag aaatcaacta tccgtggctg ttccgcctgt tcgatgaggt gggatatcag
    58861 ggctggatcg gttgtgaata taaacctcgt ggcctcaccg aagaagggct tggctggttt
    58921 gacgcctggc gctaattcgt taaatcccta attacaacgt acccatacat ccccccatct
    58981 acggatgggg taggttaact ataactttca gacagggttt cccatgtcca caattacatt
    59041 gttatgcatt gcgttagctg gcgtaatcat gctgttgctg ctggtcatca aggcaaaggt
    59101 acaaccattc gttgctctgc tcctcgtcag cctgttagtc gcacttgcgg cgggtatacc
    59161 ggcgggcgaa gtgggtaaag tgatgatcgc cgggatgggc ggtgtgcttg gttccgtcac
    59221 tattattatt ggtctgggcg ctatgctggg gcgtatgatc gaacactctg gtggtgcaga
    59281 gtcactggct aattatttca gtcgcaagtt aggtgacaaa cgaactatcg ctgcgctgac
    59341 tctggcagcg ttcttcctcg gtattcccgt cttctttgat gtcagcttta ttattcttgc
    59401 gccaatcatt tacggttttg ccaaggttgc caaaatatcg ccactcaaat ttggcctgcc
    59461 tgtcgctgga atcatgctaa ctgttcatgt ggcagtaccg ccgcatccag gccctgtcgc
    59521 cacagcgggg ttactccacg cagacatcgg ctggctaacc atcatcggta ttgcgatttc
    59581 tattcccgta gggattgttg gctactttgc agcgaaaata atcaataagc gccaatatgc
    59641 gatgtcagta gaagtactgg aacagatgca actggctccg gccagtgagg aaggcgcgac
    59701 aaaattaagc gataaaataa atccaccggg cgtcgcgctg gtcacctcgc taattgttat
    59761 tcctatcgcg attatcatga cgggtacggt ttccgcaaca ctgatgccgc cttcgcatcc
    59821 cctgcttggt acgctacagc tgatcggctc accaatggta gctctaatga ttgcgctggt
    59881 gctggcattc tggttattgg ctttacgtcg cggctggagc ttacaacaca ccagcgacat
    59941 tatgggctca gcccttccca ctgcggcggt agtgattttg gttactggtg ctggaggggt
    60001 atttggcaaa gtgctggtgg aatcgggcgt cggcaaagcc cttgccaata tgctacaaat
    60061 gattgacctg cctctgttac cagccgcatt tattatttca ctggctctgc gtgcatcgca
    60121 ggggtcagca accgtagcaa tcctgacaac cggcgggtta ctctcagaag cggtgatggg
    60181 attgaatccg attcagtgcg tattggtgac gctggcagcc tgctttggtg ggcttggcgc
    60241 gtcacatatt aatgactcag ggttctggat tgtgaccaaa tatctggggt tgtcggtagc
    60301 agacggtctg aaaacctgga cagtgttaac gaccattctc ggttttaccg gattcttaat
    60361 tacctggtgc gtatgggcgg taatttgacc ttaataaaaa ggtccgatgg gcatcggacc
    60421 ttttattgtg cacagaaaag gccagcctcg cttgagactg gcctttctga cagatgctta
    60481 cttactcgcg gaacagcgct tcgatattca gcccctgcgt ttgcaggatt tcacgcaaac
    60541 ggcgcaggcc ttcaacctga atctggcgaa cacgttcacg ggtgaggcca atttcacgac
    60601 ctacatcttc cagtgttgcc gcttcgtacc ccagcaaacc gaatcgacgt gccagcactt
    60661 cacgctgttt ggcgttcagc tcgaacagcc atttgacgat gctctgcttc atatcgtcat
    60721 cttgcgtggt atcttccgga ccgttctctt tttcatcggc caggatgtcc agcaacgctt
    60781 tttcggaatc accacccagc ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca
    60841 tacggctgac gtcatcaact ggcttatcca gttgctctgc gatctcttcc gcacttggtt
    60901 catggtccag cttatgggac aactcacgtg cggttcgcag gtaaacgttc agctccttta
    60961 cgatgtgaat cggcaaacga atagtacggg tttggttcat aatcgcccgt tcaatcgtct
    61021 ggcgaatcca ccaggttgcg tatgttgaga agcggaaacc acgttccggg tcaaacttct
    61081 ctaccgcgcg gatcagcccc aggttgccct cttcgataag gtccagcaac gccagaccac
    61141 gattgccata acggcgggca atttttacca ccagacgcaa gttactctcg atcatccggc
    61201 ggcgagaggc gacatctcca cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg
    61261 ttaacagtgg tgaataacca atctcaccaa ggtaaagctg agtcgcgtcc aacactcgct
    61321 gtgtggctcc ctgcgataac agttcctctt cggccaaatc gttatcactg ggttcctctt
    61381 ctactaaggc cttttcgtca aaaacctcaa ctccgttctc atcaaattcc gcatcttcat
    61441 ttaaatcatg aactttcagc gtattctgac tcataaggtg gctcctaccc gtgatccctt
    61501 gacggaacat tcaagcaaaa gcctggttcc gccgatttat cgctgcggca aataacgcag
    61561 cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc
    61621 ggtgctaccc atggttgcta ttttttgccc cgccttcact tcttgttgtt cccggaccag
    61681 cattgtgtcg ttatgggcgt aggcactcag gtaatcatca ttatgtttga tgataatcag
    61741 attaccgtag ccgcgcagcg cgttaccggc ataaacaacg cggccatctg cggtcgcgat
    61801 aattgcctgt cctttgctgc ctgcgatatc aatccccttg ttgcccccct cagaagcgcc
    61861 aaaggtttcg atcactttgc cctcagtcgg ccagcgccag gtggagatag gcgtactggt
    61921 tgatgtactg ctgacagttg gctcggttgt gcttgctgtt ggtaccgtta caggcgctgt
    61981 gaccgtggtc gcagctggct tgttgttcgg caacattttg ttagcactct gttcacccga
    62041 agattcagaa tacgtaattg tcggttgcga cgcaacagca acggtggaat tttgtgcagt
    62101 cttgatcaca actccttgct ctgctgcgtc ggcctgggta atggcatttc cgccagtgat
    62161 tggcgtaccg gaagcattac ccacctgcaa ggtctgacca acgttcagcg cgtatggtgc
    62221 ctgaatattg ttgcgctgag caaggtcacg gaaatcgttg ccagtaatcc aggcgatata
    62281 gaaaagtgtg tcgccttttt tcacggtata ggtactgccg ctataactgc ctttcggaat
    62341 gttcccatac tgacggttat agacgatgcg tccgttttcc atctgtaccg gctgctgagc
    62401 tactggctgc actggctgga tttgcggttg ctgggctgta gacgtcgtcc ccattttcgg
    62461 cggcggcgta atcaacatac cagaattggt atttgcaggc gcattgccat taacggagct
    62521 gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg aaaccagtga
    62581 caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt atcccccagg
    62641 aaaaattggt taataaccag tgacataatt accgtgcaag gcaccctact gaacactgga
    62701 aaagatgttc acgatacgct gacctgcggc aaaataacca ggaaaaatcc aggtatttcc
    62761 tcacgtttta agccagctcc cccttcacta aagggacaaa gcgcacggcc tccacggtat
    62821 cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc tcctccccta
    62881 cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt ggaatttccg
    62941 gcggtgccgc cgtaacaatg atagcgtcaa acggcgcgcg tgcctgccaa ccttgccatc
    63001 catcgccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg cgacgcgcct
    63061 gccactgcaa acctttaatc cgttcgaccg agcaaacatg ctggacaaga tgcgccagga
    63121 ttgccgtttg atatcccgaa ccggtgccaa tttccagcac ccgcgactgc ggcgtcagct
    63181 cgagtaattc ggtcattcgc gccaccatat acggctgcga aattgtctgc ccctgaccta
    63241 tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg aatttttcac
    63301 gcggcacggc agcaagtgca ttcagcacct gctcatcctg aataccttgc gcacgtaatt
    63361 gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca cgctgtttaa
    63421 ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca gcggcgtgat
    63481 ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat cacatttacc
    63541 gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg ggatcacctg
    63601 atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt gatccaaggg
    63661 taaatccgga acgttaatat taagaatacg tccggtgcgc agcggctctt tacacagtgc
    63721 gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc cgtcaagcga
    63781 gacggccagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta ccgtaccgga
    63841 ataaataacg tcatccccca gattcggccc ggcgttaatg ccggacacaa caatgtccgg
    63901 gcgcggacgc atcagagcat tcacgccaag atagacgcaa tcggtcgggg ttcccatttg
    63961 cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca gtgtcagaga
    64021 atttgaagcg ccgctgcggt tacgatcggg ggcgaccacc tgaacgtcag caaactcacg
    64081 caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat tactcagcaa
    64141 tatgcgcata atcacctgtt gtgttgataa gttccctgac aacgctggtt gcaaaactac
    64201 ccgccggaag ccagaaacgg atctctacgg tgacgtcatc ccaccaattc cagcttaatt
    64261 gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc accagtaaag
    64321 cttgtaattc agtttctgcg gcgacagctg cttgttcgaa tgccagcgct ttacgctgag
    64381 ttccccattc gccactgccc ggcaatgcgg cggttatcat cagctcttta tcgttgacgc
    64441 gatgctgtaa ttccgccagt tcttcggtgg ttgctacaaa ccagctacca cgtccggcta
    64501 attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg cgctcagcaa
    64561 caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt ttattgcgat
    64621 cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc ctgcaagttg ctaccgccaa
    64681 tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tacgcaaata tcgatcagac
    64741 gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag gcgttacctt
    64801 tcagcgcgcc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc tggcagcctt
    64861 ccagttgaaa ggcgctcaga tcgggcattt ccttgcctgg cacgcgagcg cataaccact
    64921 gttccgtaac agcatgtttg tctttttgcc cagcgaagct gacttcacgg gcatgaattt
    64981 tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt ttgaggattc
    65041 taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc accaccacaa
    65101 agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg aggtaagtga
    65161 gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt cacaggcaat
    65221 cccttcccca cgtccggtaa atccaagttt ttccgtagta gtggctttca cgttaacatc
    65281 atcaatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa tgtgcggcaa
    65341 catcttcggt gcctgagcga tgatagtgac atcgacgttg cccagggtat aacccttcgc
    65401 ctgaatacga cgccaggctt cgcgtagcag ctcgcggctg tcggcacctt taaatgccgg
    65461 atcggtatcc gggaacagct tgccgatatc ccccagcgcc gccgcgccaa gcaatgcatc
    65521 ggtcaacgca tggagcgcca cgtcgccatc agaatgcgcc agcaatcctt tttcgtaagg
    65581 aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcatgta cgtcaaaacc
    65641 gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta aaactcggcc
    65701 agtgccaaat cttccgggcg cgtgacttta atgttatccg cacggccttc gaccaactga
    65761 ggatggaatc cgcaatattc cagcgccgag gcttcgtcgg taatagtcgc gccttcattt
    65821 agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg cgtcagcgcg
    65881 tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg ttcggcacgt
    65941 ttcatcgtat cgcgcactgg tgcggctagg atccctcccg tgcggctggt ttcgctcaac
    66001 gccaacaatc gcgcgaggtc atcctgatgc agacaaggac gagcggcgtc atgcaccaat
    66061 acccactgcg cgtcgccagc ggctttcaaa cctgccagca cggaatcggc acgctcatca
    66121 ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc aaaacggcta
    66181 tcgccaggac ttatggcaat gacgacacgt ttcacccggg gatgcgccag cagcgcatgc
    66241 accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg acattccgtt
    66301 tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa atgagtggtt
    66361 gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg tgcgcgcttc
    66421 gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct gagttcatta
    66481 cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc ggcaaaaagt
    66541 tgatcgtttc gcgctttaag tttcgcgttt gtagcttgct gtgccgccac atcatcattg
    66601 acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg tagccagacc
    66661 agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa acggcatcat
    66721 catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg acaacgcggg
    66781 caaatgtacc acatttgtcc attgttacgt atacctaggg cgtgcagaac ataatctcat
    66841 tattagttac ggtttgaatt atgaacagag gagacaagaa agtacaaatt agcccagtag
    66901 ccacataaac agtgcgccaa acataatgcc tactgtcacc agggtgaaaa caatactgta
    66961 gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg cgacaggcat
    67021 cagtgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag agccataaat
    67081 caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg caccaggtag
    67141 tgaccatgtg gtttcttcat cctcagtaag gctgtcgtta tttgttagtg taatgttatg
    67201 gctattacgc atatttgatc ctgttacttt gacgaaccgg gcatggaaac ccggtggtgt
    67261 ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt accaaatttg
    67321 ttactaattg ttcaccattg agatgaattt ctgccgattc aggcgcttcg taaacggaat
    67381 ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag cctttgggat
    67441 cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcgataaag cgcccttctc
    67501 ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat gcggtcagca
    67561 ccaccagtcc ggcttcaacc atcaaattcg ccacttcacc gacgcgacgg atattctctt
    67621 tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca ttgtcgccat
    67681 ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc ccggcgaccg
    67741 ttgatttacc ggacccagag aggccggtaa accacagcac tacaccacga tgaccgtggt
    67801 gtagctcgcg ttgttgcaca gtgaccggat ggctatgcca gacgacgttt tcgtcatgca
    67861 gcgccattat ttatccccca gcaaatcgcg cgcgccccag tgcggaaagt ggcgacgaac
    67921 cagggcattc aattccagtt cgaatgcact gaattcagat gacgcaacgg cggcctcgtt
    67981 tttcggctca tgcaccatac cggcaccaac agtcacgttg ctcaggcgat cgataaaaat
    68041 cagcccaccc gtcaccggat tttgttgata acgatctaac accagcggct cgtcaaaagt
    68101 gagatccacg aggccgatcc cattcagtgg caggttttca acttcacgct gggtaaggtt
    68161 attaatatca accggatagc gaatgccatc aacacgcgcg cgcgtcttct taccggcaat
    68221 tttgatgtcg taactctggc ccggggaaag cggctgttcc gccatccata ccacatccac
    68281 cgacgcgctc ggcaccgccg gtaacgcttc gtctgccgcc agcagcagat cgccacggct
    68341 gatgtcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaagg cttcttcgcg
    68401 atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg gcagcgcttt
    68461 gacacgttgc ccgacttcca cgcgaccgga tgccagcgtt ccggcgtagc cacgaaaatc
    68521 gagattcggg cgattaacgt actgcaccgg gaagcgcatt ggctgggcat ccaccactcg
    68581 ctggatctcc acggtttcca gcacttcgag cagtgtcgga ccgctgtacc acgacatact
    68641 ttcactttgc gatgccacgt tgtcgccttc cagtgcagag agcggcacaa agcggatatc
    68701 cagattaccc ggcagctgcc cggcaaaggt caaataatct tcacgaatat gggtgaacgt
    68761 ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat gtttgatccc
    68821 caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt tacgggcatc
    68881 gatcagtaag atcgccagtt cacatgtcga tgcgccagtt gccatattgc gagtgtactg
    68941 ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga aataacggta
    69001 agccacgtca atggtgatgc cctgttcgcg ctcagcttgc aggccgtcca ccagcagtgc
    69061 cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca gcgatgagag
    69121 ctgatcttcg tagatttggc gggtatcgtg cagcaggcga ccaatcaggg tacttttgcc
    69181 gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt gcgcaatcat
    69241 ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtgcggtgt tcatcttaaa
    69301 aatacccctg acgttttttc agctccatag accccgcctg gtcgcggtca atcacgcggc
    69361 cctgacgttc actggtggta gaaaccagca tctcttcgat gatctccggc agtgtttgtg
    69421 catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa cgcaccatcc
    69481 gttttttaat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc atcatcaaca
    69541 taccgtcgcg ttccagaacc ggacgttccg cagcgagata cagtggcaca atgtcgatat
    69601 tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc gggaagacgc
    69661 ggatgctttc acctttgtta atttgcccgt tgtagttgtg ccacagctcc gggcgctgat
    69721 ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct ttagcgcggg
    69781 atttctcttc atcacggcgc gcaccaccga aggcggcatc aaaaccgtat ttgttcagcg
    69841 cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg tgtacgaatg
    69901 gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg cagccgtagg
    69961 ctttagcagt acgatcgcgg aactcataca tctcgcggaa tttccagccg gtatcgacat
    70021 gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga tgcagcatga
    70081 cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct gccgccacct
    70141 cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt cgtttttgag
    70201 ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc gactataggg
    70261 agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac ggaataacga
    70321 tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt ttaggaagat
    70381 ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa tttgcatggt
    70441 atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct ggcgctcggc
    70501 gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt tgctaatact
    70561 caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc tgcagaaatg
    70621 ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag tgatattcgg
    70681 acatttaata gtcggtatat ttataccgcc cgcgataatc gtaagagctg gcataacgtg
    70741 acgggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat catcattatg
    70801 gcgcatctgg atacttacgc cccgctgagc gatgctgacg ccgatgccaa tctcggcggg
    70861 ctgacgttac aaggaatgga tgataacgcc gcaggtttag gtgtcatgct ggaattggca
    70921 gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac cagcggcgaa
    70981 gaggaaggga aattaggcgc tgagaattta ctcaagcgga tgagtgacac cgaaaagaaa
    71041 aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt gtatttcaac
    71101 agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc gctggcaatt
    71161 gcgcgcagtc acggaatagc cgcaacgacc aatccggggt tgaataaaaa ttatccgaaa
    71221 ggcactgggt gttgtaatga cgcagaaata ttcgacaaag cgggcattgc tgtactttcg
    71281 gtggaagcga ctaactggaa tcttgggaat aaggatggtt atcagcaacg cgcaaaaaca
    71341 gccgcattcc ctgcgggaaa tagctggcat gacgtaagac tggataatca acagcatatt
    71401 gataaggcac ttcctggaag aatagaacgt cgctgccgtg acgttatgcg gataatgcta
    71461 ccgttggtga aggagctggc gaaggcgtct tgatgggttt gaaaatggga gctgggagtt
    71521 ctaccgcagg ggcggggaat tctaagtgat atccattatc gcatccagtg cgcccggttt
    71581 atccccgctg gcgcggggaa cacactaagc atacataact gtttttaaac aaatttattc
    71641 cgcatcaaca atctaccaac taaattaaaa catttcctta tttttaaaga acggataact
    71701 tattgattat caacaggaag aaaagaaacc aaacgtaacc catccaaatc caccggaata
    71761 cgtctgtttt ctccccaggt ctgaaattca aaacccgact cggtattggt cgcccaggcc
    71821 atcaccacat ttccgcaacc agccagttgg gtaatttgct gccagatcat ctcccgaata
    71881 cgttttgatg tatcaccaac atacacaccg gcacgcactt ccagtagcca gattgcgagc
    71941 cgtccacgta agcgtggcgg gacattttct gtaacaacca cgaccatgct catcagccgc
    72001 gtcctcgatg accgctatcg cccaacgttt caggttcggg gatggctggc ggtaacatat
    72061 ccggcgcagg ttgtggcggt tcaatttcac ctgcggcaag gacttcctca attaacggta
    72121 ttaatttgcc cgttaactta gtgctacgga aaatatcacg acaggctaat ctgacttctt
    72181 tatcaggttc tgcgggttgc ctcgccgcta tttcaaatgc ctttggtaca accgaatcaa
    72241 atttaatgat atcggctatg tcataaacaa atgaaagcgg tttgccacta tggataaacc
    72301 caatagcggg cgcatatccc gcggctaata ctgccgcttc agatataccg tacagacatg
    72361 atgtagcagc actgatgcag cgattcacaa catcgccttt ttcccagtct ttaggatcgt
    72421 atttgcgacc attccatttc acaccatatt gtttcgccag taatgcatac gtctggcgaa
    72481 cgcgagatcc ctcaattcca cgtagctgct ccactgaacg gcgagctggc ggtggctcac
    72541 gaaaacgtaa ttcatacatt ttgcgcacca ccttcagacg tagatcttcc gttaaagcca
    72601 gctttgcctg gtagagtaac ttatcagcac gcgccccacc gggttgtccg gaagagtaga
    72661 cgcgaacacc cgcttcaccg acccagacct gtagcgtccc caccgtggcg gccagatgca
    72721 ccgccgcgtg ggaaactctc gttcccggtt cgagcataat gcaggcgacc gatcccaccg
    72781 gaatgtgcgt gcggatcccg gttttatcga tcagcacgaa agcgccgtcc agtacgtcga
    72841 tttgcccgta ctggaggaag atcatagagg tgcgatcttt taacgggatc gggctcagcg
    72901 gtacaaacgt cacgcctctg ctccgggttt gatcagcatc agaccacagc cgaacgcgcg
    72961 gcttttgcca tacccctgac tgagacgttg cagaaataac ccgggatcgg tgaccgtaag
    73021 catccccgta taatccacgc tactgaactg gatcagttgc cgggagtttt cccgccgcag
    73081 ttgctgttgt cggtaggcat caaccgagat atccagcagt gtaaaaccac ttctctcccc
    73141 ctgcgctgcc agccagtcca gcgccgcctg ttgttgatgc aaccagacat cacttccttc
    73201 cgcctgcccc ctcacctgcc gtttcgcctc catcagcaga tcgtggcgct tgcccgcttt
    73261 acagattgtt ggattcgccc gcaggttgaa acaaagttgt tgtccggtac acagctcggg
    73321 cgcaaatgac cggcattcta tggtgaacgt atcactttcc gccgggcgtt cctgcgacag
    73381 tacaaaaaag cgaaacgcgc cctggagttc ttcccgacga taaagaaatt gcctttcttt
    73441 gccgctaggg aagagatccc acagccactg atgcatcaca tattcaccgc gatccaccag
    73501 atgcagcaac tgcgcaggcg aaagctggcc ggtatgtaag gttattcttg agaggtacat
    73561 ggttcctcct tgctgagcca cggcccctga ttgacggtgc gttccccaaa cagccactgc
    73621 tgacgattta aaggaacatc ccggcggcgt aatattttgc tcgcgaccag gccgtcgtgt
    73681 ttcccttccc accagcattc atcctgaagt ttcgggagtg aaactttcag tttatggaaa
    73741 tgatcctgat actgttggta tgcattacat aaggcatcac acgcgttgcc ttcgagcagt
    73801 aacggcgcaa gaggtaatgc cagcggatga ctttttcgcc ccagataaag cggaaaaact
    73861 ggatggcgta aaccgtcctg caactgttcg aggctgtaag gcgcatcggc tgttgttgcc
    73921 accgccacca tccaccaggc atcggtgtag tagtcgcgcc gggagataat cgcgctcaaa
    73981 agctcagggt cgctcaactc ttcgcgacgg ctgaaataac gtgctttacg cacctctttt
    74041 ggcatctgga ccgtgtgata atcccgtgcc cagcgcgggt tacggctggc gcaaaccacc
    74101 agtgaatagt ggcgattaaa cgcgtttaag cgttcggtat catcacgccg gatccctacc
    74161 ccggcagcca gcagccccag caatgctgag agcgaaggca gttcatgggt atgacgcact
    74221 tcgccggggg catcgacgcc ccaggatgcc atcggcccat gaagctgaaa aatcagatat
    74281 tggctcataa gccgcccctt acgcgcagat aaagtccagc acgtccttca tgcttccctg
    74341 tttgttcatc acgtcaaaac ttgcgtaatc ggtcttctgt tcgtagaccg tattcatatt
    74401 ttcgcgaaga gttgtaatac gctgcaccgc cacctctaac tgacgggtgc cattaatggg
    74461 ttcatagaaa gctgccgcca gagaacgcgg ttgatcggtg cctttttctg ccagcgccca
    74521 ggaggcataa gcccggctgg caaagctgtt ttgtttaccg gttggggaga ctttaagcgc
    74581 ggcttccgta aaggcgcgta tggtctgatt agctaacgct tcgtcaccgc cgaggttttc
    74641 taccagcaga tctttatcaa tgcagataag atataggtgt agaacagcgc agaaccgaat
    74701 ccggtttcat ctacatgacc ggcaccggca tcttcagaag cctggcgtaa atcatctacg
    74761 gcggtgaaaa aatcattttc gacaatcgtt tcactgacac caaacgcatg cgcgacctgg
    74821 caggcggctt ctacattaaa cccgggtttt ttcgccagca tacgaccaaa catagcgata
    74881 tctaccgcca tacggtcttt acgtaacaaa gcgagatctt tctcttttgg cgcgcgcttt
    74941 tcttctgcca gttgatgggc cagcgctttt acggcgtcaa attctgccgg gctgatatgg
    75001 acgagctgtt cagtttcggc gctagtgagc ggatcttttg gttttttgtc gtttttttgt
    75061 tttttgtctt ttttggcttt cccaagataa tcggctatct ctactgccca ttcgatggct
    75121 tttttatctt cgattccttt ctcaatcagg atagttgccg cctcacgcgc aatacgcccg
    75181 ctacgaacac caatatggcc cgccagcgcc tgttcaaaaa gttcagaagt gcgccaagca
    75241 cgcttcagac tttgcgagga aacgcgcaga cgcgttgctc cacccagaat cacagttttc
    75301 ggagagccgg aatcatcacg gttaagattg gcagcagggt aagcggttaa caaatgaagc
    75361 tgaataaatg ttgtcatgag atagtccttt attaattttg ttcgttatcg gcgtcgccag
    75421 cctggtaata ttccagcgcc cagcgcccag cgcccagcgc ccagcgccca gcgcccagcg
    75481 gatacggata aattctgttg gtcgctgctg gcggcggtga tgattgagca ggtcatcgct
    75541 ctcctggcac cagcggaaga ccccctcttc tagagagtca agattgacgg aaccgtttaa
    75601 taatttgacc gcacggcgta actgccgcaa taaatcatcc ggcgttttta ctgccaacaa
    75661 acgagtaaac cgcccctttt tcattaccgc cgccagttgc gcggcaaaag gctgccgttc
    75721 gtcaatagct ttaacattgg cgctaactgc tgctatcagc gctagtgcgg tgatacgcca
    75781 ctcgggttca tcctgccact ttatttgtct gttcttaaga aacaggcgaa atccatccgt
    75841 cagacaaaca tcattaaccg tcgtactacg ccgcagactg gcacgctcgc cgcgtttatc
    75901 ctgcaactct tcatgccatt tacgcagcgc aactttatgc tcatctttta caatactcat
    75961 tcagcagcct cctgcttttt ctccctggcg gcgttagcat tttgcttctc cgctgatgtt
    76021 gtaaaatatt tcttgcgtgc ggtcatgacg cgttccaaat caacgggctc atatggattg
    76081 gtaaagacac gatcgtcaaa atcctgacgc gcgaataacc acatttcctt ttgccattta
    76141 ctgagtaatt cgtccggatc ctgaccttct tcaattttgc gcactaatct caggaatcga
    76201 tgctgagttt tgttccagaa gtcaatatcc acaaaaccga aatcaagctt tgtggttttc
    76261 ttctcagaga accatgcttc tttcaacgct cggtgtaaca agcttagaat gtgtgatgcc
    76321 gcttgcgctg ccagacgcaa cttaggtatt aggtcttctt ttttgtttaa caacagcggg
    76381 aaatggtgtt cataccaaca acgaaccttc atatcttcaa aatcaaaacc aaatccccat
    76441 aagcccaccc ttgtctgctt tagattgctg gcattcaaaa gtttcacgac ctgtgcgggg
    76501 agttcagtat tgttttttga gttacctacc tctatgaggc ctaaccagtc gcgccagatt
    76561 aaaccaccag gttgtggttt aactgagtaa aaatctccac cctctttaag cggcagacgg
    76621 taaggtgtta gaggatggcg ccacatgacg tattgaacac catagttttt gagtgacatc
    76681 aagcccaaaa gagcatcgct ctgcctaccg caaatgtcgc agttacctat tgaggttgtc
    76741 ttaaaatcaa tacgtatacg tcgtggcatt ccccagtagg cctgaagttt attgacttgt
    76801 tcatccgtta ccacagcacc gtccagttcg ctggtacgcg tcggtgcaag ccaaggaaag
    76861 accaggtcat caaaggtttt tggtaaaggt aaatcggctt catcttgcgg cattacgttt
    76921 agccacagct ttcgccacaa aggggtttgt tgattgccct gatactccag caattctata
    76981 agtgtcgtca gaggccctcc gccacgtagc ccaggatagt aaccgcgtcc acctataggt
    77041 gcattcaact ggattgaaaa aagcgctaaa ggcacacaat gcaggcacag atgctgagtt
    77101 gtgtctcgct taataaaatg gtctgtattt cgttcttttg tttgttttcc tggtgcgtca
    77161 ggaagaagcg aagcaataaa agttgcctta acttttaatt catcaaaatc ctgcataaat
    77221 gaaggggaat ctggcccaaa ctgaaaagca tgttctaaag acagcaatgc ttcccttagc
    77281 ttttcagctt ccagtccgtc ttcccagata tcatcccaac tacgttgatc ttttggcgcg
    77341 aagctggttt gtagtagccc cagcaaaaac tgccaggccg ccccctggag atctgcccgc
    77401 ggcgcagcga tatcgacaac attttcatca gccagatcga ctggtgccag tttgcctgtc
    77461 gttccgtctt taaaacgaac gggcaaccac ggggttgtca gaagtgaaaa cgagttcata
    77521 cgcatcctct cgtaaaaaag accatcccag gtcggttgtc catccttcca tccttttctg
    77581 tatatcaact attcagagag tcttgtcgca gacatctcaa ccaactttgc caatcaatcc
    77641 ctccctggcc gaatacccgc aactttcgtc atcggtgact aaaatcacgt ttgccatttc
    77701 cggatcttgc cgctgttcaa cgcaccactg cctgaacgca tccccttcca gtaaagaaaa
    77761 ctcatcccga tgttttttcc accagcttcg gcgcactctg acaacgctca tttcccatgc
    77821 gtgagcaccg gtggcataag gcttcaccac accgtcaata caggtagcca gccacaggga
    77881 aacagactct tcagccagac gtgtcgacag tttttccgga aggtaatcgt tgatattggc
    77941 ggcatagccg agcctgaagt tcagcacata ctttttagcc cttgcgcgat cacagtaata
    78001 tttgcccact tgctcctgct cgctgcgggc aaatccttcc ggcattacca cgtcctcacc
    78061 gtaaactgat tcaataagaa gccgggctgc gtgcggcatc tgaatagcgc cttgctcacg
    78121 cagcacacgc tgcgtcagcc agattcgccc atgatcggga tagacatatg cactgttacg
    78181 catggcactg ccgaaccatt cgtcaccagg agcgtcatcc cagacggggg ccagaatcag
    78241 caattcaggg ggggaacgct cgtcttttcc gtcacgcttt aactgaccat taatatcgcg
    78301 gatatgccgc tgtaatcgcc ccgctcgctg gattagcaaa tcaatggggg ccaggtcgga
    78361 gatcatttcg tccaggtcaa tatccacgct ttgttcaatt acttgtgtac aaacaatgac
    78421 ttgagccgct ctctgtaaac tacaatattt accaaagcgt gccagcgttt ccgtttcaat
    78481 tcgctggcga tcgctaaaag caaagcggct atgaaaaagt gaaaggctgg aagcaggaat
    78541 gacgccgcgg gcaagcagct gacgatacac ctgaatagcg tcatcaacag aattccgaat
    78601 ccaggcgatg catttcccct gacttaccgc cgattcgata cgcgcaatac actcttgttc
    78661 actatggagc caacccacgc tcacgctacg ttcaacttct ttgcgcgtcg ctacccggtg
    78721 cgaatgtaca tcggttttcg tgacatgcgt cagccagggg taatcatctt ttcccaggag
    78781 tggggcttct tgctggccct ctgcaccacg cgcaaaggcg gcgacgagtt tgtcgcgctg
    78841 ctgttgagat aacgtagcag aaagcaaaat aacgctgttt ccgccacgcg cctgccgctc
    78901 gatcagccct tcaagaatgc acgacatata agcgtcacag gcgtggatct catcagccag
    78961 caggattttg ttactcagcc ccagaagccg cagattatta tgtttaaacg gcatcactgc
    79021 catcatcgcc tgatccaggg tgccgacgcc aatttcagcc agcaacgcct tcttgttact
    79081 gtcggcaaac taggccgcac aaccctggct gaaagcttgt tcatccggtt cttctgaccc
    79141 gattaaatca ccggaccaga g
//
LOCUS       DAJVTY010000003        68964 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVTY010000003 DAJVTY010000000
VERSION     DAJVTY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68964
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.003"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..384
                     /locus_tag="OQQ66_000184"
     CDS             <1..384
                     /locus_tag="OQQ66_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1903239.1"
                     /translation="INGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMRIDGT
                     TGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAW
                     PVNLVGGELETGKFQGTATITVMVR"
     gene            complement(427..1287)
                     /gene="rsmI"
                     /locus_tag="OQQ66_000185"
     CDS             complement(427..1287)
                     /gene="rsmI"
                     /locus_tag="OQQ66_000185"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1903240.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1351..3378
                     /gene="lpoA"
                     /locus_tag="OQQ66_000186"
     CDS             1351..3378
                     /gene="lpoA"
                     /locus_tag="OQQ66_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1903241.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTMPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3336..3731
                     /locus_tag="OQQ66_000187"
     CDS             3336..3731
                     /locus_tag="OQQ66_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1903242.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3751..4341
                     /gene="diaA"
                     /locus_tag="OQQ66_000188"
     CDS             3751..4341
                     /gene="diaA"
                     /locus_tag="OQQ66_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1903243.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4351..4926
                     /gene="yraP"
                     /locus_tag="OQQ66_000189"
     CDS             4351..4926
                     /gene="yraP"
                     /locus_tag="OQQ66_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1903244.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5040..6080)
                     /locus_tag="OQQ66_000190"
     CDS             complement(5040..6080)
                     /locus_tag="OQQ66_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1903245.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAICLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEARSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6153..6788)
                     /locus_tag="OQQ66_000191"
     CDS             complement(6153..6788)
                     /locus_tag="OQQ66_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1903246.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6916..7434
                     /gene="yhbO"
                     /locus_tag="OQQ66_000192"
     CDS             6916..7434
                     /gene="yhbO"
                     /locus_tag="OQQ66_000192"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1903247.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7414..7857)
                     /locus_tag="OQQ66_000193"
     CDS             complement(7414..7857)
                     /locus_tag="OQQ66_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1903248.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7908..8210
                     /gene="yhbQ"
                     /locus_tag="OQQ66_000194"
     CDS             7908..8210
                     /gene="yhbQ"
                     /locus_tag="OQQ66_000194"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1903249.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8197..8700)
                     /locus_tag="OQQ66_000195"
     CDS             complement(8197..8700)
                     /locus_tag="OQQ66_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1903250.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8694..9218)
                     /locus_tag="OQQ66_000196"
     CDS             complement(8694..9218)
                     /locus_tag="OQQ66_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1903251.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9427..10407
                     /locus_tag="OQQ66_000197"
     CDS             9427..10407
                     /locus_tag="OQQ66_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1903252.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10416..11294
                     /locus_tag="OQQ66_000198"
     CDS             10416..11294
                     /locus_tag="OQQ66_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1903253.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11500..12507
                     /locus_tag="OQQ66_000199"
     CDS             11500..12507
                     /locus_tag="OQQ66_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS1903254.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12625..13869)
                     /gene="mtr"
                     /locus_tag="OQQ66_000200"
     CDS             complement(12625..13869)
                     /gene="mtr"
                     /locus_tag="OQQ66_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1903255.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14023..15912)
                     /gene="deaD"
                     /locus_tag="OQQ66_000201"
     CDS             complement(14023..15912)
                     /gene="deaD"
                     /locus_tag="OQQ66_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1903256.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15905..15985)
                     /gene="yrbN"
                     /locus_tag="OQQ66_000202"
     CDS             complement(15905..15985)
                     /gene="yrbN"
                     /locus_tag="OQQ66_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1903257.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16092..16976)
                     /gene="nlpI"
                     /locus_tag="OQQ66_000203"
     CDS             complement(16092..16976)
                     /gene="nlpI"
                     /locus_tag="OQQ66_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1903258.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17085..19220)
                     /gene="pnp"
                     /locus_tag="OQQ66_000204"
     CDS             complement(17085..19220)
                     /gene="pnp"
                     /locus_tag="OQQ66_000204"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1903259.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19468..19737)
                     /gene="rpsO"
                     /locus_tag="OQQ66_000205"
     CDS             complement(19468..19737)
                     /gene="rpsO"
                     /locus_tag="OQQ66_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1903260.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19886..20830)
                     /gene="truB"
                     /locus_tag="OQQ66_000206"
     CDS             complement(19886..20830)
                     /gene="truB"
                     /locus_tag="OQQ66_000206"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1903261.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20830..21231)
                     /gene="rbfA"
                     /locus_tag="OQQ66_000207"
     CDS             complement(20830..21231)
                     /gene="rbfA"
                     /locus_tag="OQQ66_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1903262.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21395..24043)
                     /gene="infB"
                     /locus_tag="OQQ66_000208"
     CDS             complement(21395..24043)
                     /gene="infB"
                     /locus_tag="OQQ66_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1903263.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24068..25555)
                     /gene="nusA"
                     /locus_tag="OQQ66_000209"
     CDS             complement(24068..25555)
                     /gene="nusA"
                     /locus_tag="OQQ66_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1903264.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25583..26035)
                     /gene="rimP"
                     /locus_tag="OQQ66_000210"
     CDS             complement(25583..26035)
                     /gene="rimP"
                     /locus_tag="OQQ66_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1903265.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26242..26318)
                     /locus_tag="OQQ66_000211"
     tRNA            complement(26242..26318)
                     /locus_tag="OQQ66_000211"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26282..26284),aa:Met,seq:cat)
     gene            26666..28009
                     /gene="argG"
                     /locus_tag="OQQ66_000212"
     CDS             26666..28009
                     /gene="argG"
                     /locus_tag="OQQ66_000212"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1903266.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(28017..29642)
                     /locus_tag="OQQ66_000213"
     CDS             complement(28017..29642)
                     /locus_tag="OQQ66_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1903267.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISSAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPPEGQG"
     gene            complement(30101..30187)
                     /locus_tag="OQQ66_000214"
     tRNA            complement(30101..30187)
                     /locus_tag="OQQ66_000214"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30151..30153),aa:Leu,seq:gag)
     gene            complement(30202..30534)
                     /gene="secG"
                     /locus_tag="OQQ66_000215"
     CDS             complement(30202..30534)
                     /gene="secG"
                     /locus_tag="OQQ66_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1903268.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30762..32099)
                     /gene="glmM"
                     /locus_tag="OQQ66_000216"
     CDS             complement(30762..32099)
                     /gene="glmM"
                     /locus_tag="OQQ66_000216"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1903269.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIISKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32092..32940)
                     /gene="folP"
                     /locus_tag="OQQ66_000217"
     CDS             complement(32092..32940)
                     /gene="folP"
                     /locus_tag="OQQ66_000217"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1903270.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33030..34964)
                     /gene="ftsH"
                     /locus_tag="OQQ66_000218"
     CDS             complement(33030..34964)
                     /gene="ftsH"
                     /locus_tag="OQQ66_000218"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1903271.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35064..35693)
                     /gene="rlmE"
                     /locus_tag="OQQ66_000219"
     CDS             complement(35064..35693)
                     /gene="rlmE"
                     /locus_tag="OQQ66_000219"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1903272.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35819..36112
                     /gene="yhbY"
                     /locus_tag="OQQ66_000220"
     CDS             35819..36112
                     /gene="yhbY"
                     /locus_tag="OQQ66_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1903273.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36269..36745)
                     /gene="greA"
                     /locus_tag="OQQ66_000221"
     CDS             complement(36269..36745)
                     /gene="greA"
                     /locus_tag="OQQ66_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1903274.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36993..38426
                     /gene="dacB"
                     /locus_tag="OQQ66_000222"
     CDS             36993..38426
                     /gene="dacB"
                     /locus_tag="OQQ66_000222"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1903275.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38466..39638)
                     /gene="cgtA"
                     /locus_tag="OQQ66_000223"
     CDS             complement(38466..39638)
                     /gene="cgtA"
                     /locus_tag="OQQ66_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1903276.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39654..40619)
                     /locus_tag="OQQ66_000224"
     CDS             complement(39654..40619)
                     /locus_tag="OQQ66_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1903277.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40746..41003)
                     /gene="rpmA"
                     /locus_tag="OQQ66_000225"
     CDS             complement(40746..41003)
                     /gene="rpmA"
                     /locus_tag="OQQ66_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1903278.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(41024..41335)
                     /gene="rplU"
                     /locus_tag="OQQ66_000226"
     CDS             complement(41024..41335)
                     /gene="rplU"
                     /locus_tag="OQQ66_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1903279.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41594..42565
                     /gene="ispB"
                     /locus_tag="OQQ66_000227"
     CDS             41594..42565
                     /gene="ispB"
                     /locus_tag="OQQ66_000227"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1903280.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42794..43072
                     /gene="sfsB"
                     /locus_tag="OQQ66_000228"
     CDS             42794..43072
                     /gene="sfsB"
                     /locus_tag="OQQ66_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1903281.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43120..44379)
                     /gene="murA"
                     /locus_tag="OQQ66_000229"
     CDS             complement(43120..44379)
                     /gene="murA"
                     /locus_tag="OQQ66_000229"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1903282.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44434..44688)
                     /gene="ibaG"
                     /locus_tag="OQQ66_000230"
     CDS             complement(44434..44688)
                     /gene="ibaG"
                     /locus_tag="OQQ66_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1903283.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44848..45141)
                     /gene="mlaB"
                     /locus_tag="OQQ66_000231"
     CDS             complement(44848..45141)
                     /gene="mlaB"
                     /locus_tag="OQQ66_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1903284.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLQLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45141..45776)
                     /gene="mlaC"
                     /locus_tag="OQQ66_000232"
     CDS             complement(45141..45776)
                     /gene="mlaC"
                     /locus_tag="OQQ66_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1903285.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45795..46346)
                     /gene="mlaD"
                     /locus_tag="OQQ66_000233"
     CDS             complement(45795..46346)
                     /gene="mlaD"
                     /locus_tag="OQQ66_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1903286.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46351..47133)
                     /gene="mlaE"
                     /locus_tag="OQQ66_000234"
     CDS             complement(46351..47133)
                     /gene="mlaE"
                     /locus_tag="OQQ66_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1903287.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47141..47950)
                     /gene="mlaF"
                     /locus_tag="OQQ66_000235"
     CDS             complement(47141..47950)
                     /gene="mlaF"
                     /locus_tag="OQQ66_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1903288.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48160..49137
                     /locus_tag="OQQ66_000236"
     CDS             48160..49137
                     /locus_tag="OQQ66_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1903289.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49151..50137
                     /gene="kdsD"
                     /locus_tag="OQQ66_000237"
     CDS             49151..50137
                     /gene="kdsD"
                     /locus_tag="OQQ66_000237"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1903290.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50158..50724
                     /gene="kdsC"
                     /locus_tag="OQQ66_000238"
     CDS             50158..50724
                     /gene="kdsC"
                     /locus_tag="OQQ66_000238"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1903291.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50721..51296
                     /gene="lptC"
                     /locus_tag="OQQ66_000239"
     CDS             50721..51296
                     /gene="lptC"
                     /locus_tag="OQQ66_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1903292.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51265..51822
                     /gene="lptA"
                     /locus_tag="OQQ66_000240"
     CDS             51265..51822
                     /gene="lptA"
                     /locus_tag="OQQ66_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1903293.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51829..52554
                     /gene="lptB"
                     /locus_tag="OQQ66_000241"
     CDS             51829..52554
                     /gene="lptB"
                     /locus_tag="OQQ66_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1903294.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52602..54035
                     /gene="rpoN"
                     /locus_tag="OQQ66_000242"
     CDS             52602..54035
                     /gene="rpoN"
                     /locus_tag="OQQ66_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1903295.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54058..54345
                     /gene="hpf"
                     /locus_tag="OQQ66_000243"
     CDS             54058..54345
                     /gene="hpf"
                     /locus_tag="OQQ66_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1903296.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54463..54954
                     /gene="ptsN"
                     /locus_tag="OQQ66_000244"
     CDS             54463..54954
                     /gene="ptsN"
                     /locus_tag="OQQ66_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1903297.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            55000..55854
                     /gene="rapZ"
                     /locus_tag="OQQ66_000245"
     CDS             55000..55854
                     /gene="rapZ"
                     /locus_tag="OQQ66_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1903298.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55851..56123
                     /gene="npr"
                     /locus_tag="OQQ66_000246"
     CDS             55851..56123
                     /gene="npr"
                     /locus_tag="OQQ66_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1903299.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56337..56969
                     /gene="yrbL"
                     /locus_tag="OQQ66_000247"
     CDS             56337..56969
                     /gene="yrbL"
                     /locus_tag="OQQ66_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1903300.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56966..57694)
                     /gene="mtgA"
                     /locus_tag="OQQ66_000248"
     CDS             complement(56966..57694)
                     /gene="mtgA"
                     /locus_tag="OQQ66_000248"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS1903301.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISQWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57691..58344)
                     /gene="elbB"
                     /locus_tag="OQQ66_000249"
     CDS             complement(57691..58344)
                     /gene="elbB"
                     /locus_tag="OQQ66_000249"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1903302.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58574..60910)
                     /gene="arcB"
                     /locus_tag="OQQ66_000250"
     CDS             complement(58574..60910)
                     /gene="arcB"
                     /locus_tag="OQQ66_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1903303.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(61006..61935)
                     /locus_tag="OQQ66_000251"
     CDS             complement(61006..61935)
                     /locus_tag="OQQ66_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1903304.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62610..67070
                     /gene="gltB"
                     /locus_tag="OQQ66_000252"
     CDS             62610..67070
                     /gene="gltB"
                     /locus_tag="OQQ66_000252"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1903305.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67083..68501
                     /gene="gltD"
                     /locus_tag="OQQ66_000253"
     CDS             67083..68501
                     /gene="gltD"
                     /locus_tag="OQQ66_000253"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1903306.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68685..>68964
                     /locus_tag="OQQ66_000254"
     CDS             68685..>68964
                     /locus_tag="OQQ66_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903307.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLF"
BASE COUNT        16832 a        18742 c        17159 g        16231 t
ORIGIN      
        1 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
       61 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      121 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      181 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      241 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      301 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcacagcc
      361 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      421 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
      481 aatttctgcg accagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
      541 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
      601 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gttcgccaac
      661 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
      721 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtggattc
      781 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
      841 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
      901 taacgcagtg atagcagcac acggcccggg tagcggcacc acgcgaatcc ccgcttcacg
      961 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1021 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1081 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1141 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1201 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1261 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1321 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1381 gccgcgcgtt gtctgcctgt tgttctagca gccctgattt tcgccggttg tggcacccat
     1441 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     1501 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     1561 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     1621 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     1681 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     1741 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     1801 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     1861 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     1921 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     1981 tggtttgata accgtaacga tcccgatatg atgaaagccg ggatcgccga ctggcagaaa
     2041 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2101 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2161 gtatttggtc gcactattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2221 gtggcggctc aggtagctgc cgcagacgta gcagaacaac ctcagccgca aactgcggat
     2281 ggcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca gcctgcggcc
     2341 cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc acccgcaaat
     2401 ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca gatcttaagc
     2461 caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa taacgttgaa
     2521 gagttgctta agagcaacac cccgctgaac gtgctggtac tgaaccagcc ggagaatatc
     2581 gaaaaccgcg tcaatatttg ttacttcgcg ctttcaccgg aagacgaagc gcgcgatgca
     2641 gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc acgcagttca
     2701 ttgggcgatc gcgtagccaa tgcgtttgcg caggagtggc agaaactggg cggcggcacc
     2761 gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa cggcggttct
     2821 ggtatcgctt taacgggtac cccgattact cccagagcga caaccgactc cggcatgacg
     2881 accaacaatc caacgctgca aaccatgcca accgatgacc agttcaccaa taatggcggt
     2941 cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat caaaccgatg
     3001 atcgccatgc gtaacggtag ccagagcggt gcaatgctgt acgccagctc ccgcagcgca
     3061 caaggcaccg caggcccgga tttccgtctg gagatggaag gtttgcagta cagcgaaatc
     3121 ccgatgctgg cgggcggtaa tctgccgtta atgcagcagg cactcagcgc ggtgaataac
     3181 gattattcac tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgct ggcaaatcat
     3241 ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg aagcctgacg
     3301 gcaaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca acaaggtcag
     3361 gtagtccccg ccagttaacc accaaacaga ccggcgatgc gtgggaagta caagcgcgtc
     3421 gctggctgga aggcaaagga ctgcgatttg tcgccgctaa cgtgaacgag cgtggcggtg
     3481 agatcgatct gataatgcgt gaaggctgga ccaccgtttt tgtcgaggtg cgttaccgcc
     3541 gctctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa cacaaattat
     3601 tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact gtggattgcc
     3661 ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat gcctttaatg
     3721 accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc ttgcttcact
     3781 gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat ctcccgtgca
     3841 gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg tggtaatgga
     3901 acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt cgaaacggag
     3961 cggcccagct tacctgccat tgcactaaat actgataatg ttgtcttaac ggcgattgcc
     4021 aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg tcatgcggga
     4081 gatgtattgt tagccatttc cacccgtggc aacagccgcg atattgttaa agcagttgaa
     4141 gccgccgtta cgcgtgatat gaccattgtg gcattgaccg gctatgacgg cggcgaactt
     4201 gcaggtttgt tagggccaca ggatgtggag atccgcattc cttcgcatcg tagtgctcgc
     4261 attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga taacacgctt
     4321 ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat cgcagtcctt
     4381 atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac cgctgctgtg
     4441 ggtaccaaag ccgcaactga cccacgcagt gtcggtaccc aggtggacga tggtaccctg
     4501 gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga aacccgtatt
     4561 aatgtcacgg cgtatcaggg caaagtgctg ctggttgggc agtcaccaaa tgctgaactt
     4621 tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt gtataacgag
     4681 attcgtcagg gccagccgat tggtctgggc gaagcatcta acgatacgtg gatcaccacc
     4741 aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt gaaagtgacc
     4801 accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc gaaagcggcg
     4861 gcagatattg ccagccgggt gagcggcgta aagcgggtaa ccacagcgtt tacgtttatt
     4921 aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca ggcctacaac
     4981 gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt tccctcaaat
     5041 cagaacaaca gcgccagccc gccgacaatc acaccagaca ctgccaccat cgcccctgtc
     5101 agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg caaactcact
     5161 gccgggagcg tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc cagcatcatc
     5221 gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc tgctaccgcc
     5281 atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg gaataaccag
     5341 acgcgtgcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt actccagaaa
     5401 agtgtccata gcgccctgcc ccagcggcta aaaaaaccgc tccgtgcttc cggtatgtca
     5461 atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac cagcgtcgca
     5521 atcagcaaca ccatcaccag cccggccacc agacaaatcg ccgcaaaacc ccagccgagg
     5581 acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat ccagaatgcc
     5641 agcgcaccgc ccatcgatac ctgttggcga cgcatccccg ccgcgaccgg agccgcacag
     5701 caggtacaca tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc gcgaaagcgc
     5761 gattgcccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat caacgaaccg
     5821 aggatcaccc ccagcaccgc cgctttccat actgcgagga aatagatcat cgcgtaatcc
     5881 aacgccgctt gccacggatt agcatccgcc tgcgcaagga tagatttacc gatactgtgg
     5941 gtttcggctg cagtaaacgc tttgccgtag taatgttgcc atttcacata ccagagaccg
     6001 gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggtgt tgccgcctga
     6061 gatgaagact gaccagtcat agcattccct aggaataagt tatgataatt gagcgcgtga
     6121 atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg taattctgaa
     6181 gagcttaaaa tcgtcacgcc ttcatgctca ggccgcatcg cctctgccag catgacccgc
     6241 gccacgtctc tcgcatcaat ggatttccag ttacctggta acaaacggaa cagcggcgca
     6301 aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gtaacatcga cgggcgagca
     6361 atggtcaatt tcggccagtt ctgggcaatt aatgcttctt ccatctcccc tttgacgcgg
     6421 ttatagaaaa acggcgagtg ggcattggcc cccatagcac tgaccaccag catatgctgc
     6481 gcgcccagcc gccgcccggt taatgcggta tccactacca gcgtgtaatc ggcatgaata
     6541 aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg
     6601 atgggttcgg tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg attaaacacc
     6661 ccgggcatat cgcccaacgg acgtcgcgtc ggcgcagcaa tggcgttaac tttcggttca
     6721 ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt aatcagtacc
     6781 tgactcatct tcgctccttt acagaattgt ctgccttgcg ctccacggct catgcaccag
     6841 tcttaataga ccatgaggta attatccccg attgtgggaa attcgcctca tccaatgcaa
     6901 caacgcggag gaagcatgag taagaaaatt gccgttttaa tcagtgatga atttgaggat
     6961 tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt aattaccatt
     7021 gaaaagcaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt gaccatcgat
     7081 aaatccatcg atgaagtgac gccagcggag tttgatgccc tgctgctacc gggcggccat
     7141 tcaccggatt atctgcgtgg ggacaaccgt tttgtcacct ttacccgtga ttttgtgaat
     7201 agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag cgccgatgtg
     7261 attcgggggc gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa aaatgcgggc
     7321 gcggaatttt acgatcagga agtcgtggtt gataaagatc agctggttac cagccggaca
     7381 ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc ctgagtcgcg
     7441 tagccaaatc atttttttac caaagcccag cgtgttgtcg gtgaatttga tttcatcgag
     7501 ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgat gattgtacgc
     7561 cttgcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt taaactgcac
     7621 accgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa ccgcagcctg
     7681 cggcccactc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt
     7741 ctgcgcatca aacagataaa aagcattagc gcaccacagt tccccttcct gctgcacaca
     7801 ccaggtgaca acatgttgtt ttgccaacca acggctgatg gtgatgagtg tttccattgc
     7861 tgttctcttt tatactgtgg gcctgacctt aacatgcgac gcagacgatg acaccctggt
     7921 ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc acggatgtcg
     7981 aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc gggaaaggag
     8041 aactgacgct ggcgttttct gcgccagtgg gcgaccgttc gctggcacta cgggcggaat
     8101 atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa ggcgcagggt
     8161 ttgcagaact gttgagcagc ctgcaaaccc cggagattaa aagcgattga aatgctcgtg
     8221 atactcaacc agaccggtta cgccattcag cgcgtcatcc gctaaacgat gtacctggaa
     8281 ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca gttcaaagcc
     8341 gaaacgactg tacagcgccg gatcgcccag cgtcaccact gcggcatagc cgaactcatt
     8401 aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac ggtatttttc
     8461 atcaaccgcc aacggtgcca tgccgaccca ttgcaggtct tcgccctgca catcaaccgg
     8521 actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca gccccagcgt
     8581 cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac tttcgaatga
     8641 gcgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc gaattagcat
     8701 ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc ctcaacaaaa
     8761 tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag ttcaatggcg
     8821 tccatcaggt ttttcacatg cagccccagc tccgtatcgc cttcaatcac cagccgacgc
     8881 tggaagaaga gcgtatctgg atcttgttta cgagccgcaa tcatcagcag atcgctgacg
     8941 tcggcactaa aactcacatc agcttgcgcg ttctggctga ccaccagttt gccattcacc
     9001 accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca gcggccttca
     9061 agaaactcca gctcgccatc atccagcgcc tgacggaatt gccagcttaa gacctgctca
     9121 agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa cagagatggc
     9181 cccaaatgca caatacggga acgaagttta tccaacacga gttttactcc ctgtttcaac
     9241 aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa caattccata
     9301 tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat tgtcgcagca
     9361 aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgatagcgca accttcagga
     9421 aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa
     9481 aacggcgcag atgctgttta tatcgggcta aaagatgata ccaatgcccg tcacttcgcc
     9541 ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca tcaacatcgc
     9601 cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc ccgttggcag
     9661 cgcgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc cgacctcgcc
     9721 atgctggaat acgccgccga gcgttatccg aatattgagc gccacgtatc ggtgcaggct
     9781 tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgacgt tgcccgcgtg
     9841 gtgctgccgc gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt cacacctgta
     9901 ccactggaag tgtttgcttt cggcagcctg tgcattatgt cggaaggccg ttgctatctg
     9961 tcgtcgtatc tgacgggtga gtcgcctaac accgtgggcg cgtgttctcc ggcccgtttc
    10021 gtgcgctggc aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt gctgatcgac
    10081 cgttatcagg acggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg ttatctggtg
    10141 gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct ggaactgctg
    10201 ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca gcgtagcccg
    10261 gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat
    10321 ccgcaaagcg cgtggatgga gacgctcggg tcgatgtccg aaggcacgca gaccaccctt
    10381 ggcgcgtatc accgtaaatg gcagtgagaa gagcaatgaa atattcctta gggccagtgc
    10441 tgtggtactg gccaaaagag acgctggaag atttttatca gcaggccgcc accagcagcg
    10501 ccgacgtgat ttatcttggt gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg
    10561 actggctgga gatggcaaaa tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc
    10621 tggcgctggt gcaggcatcc tctgaactgg gcgaactgaa acgctatgtt gagaacggtg
    10681 agtttctgat tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc
    10741 cgttcgtcgc cgggcacgcg ctgaactgct acaacgcggt gacgctgaaa atattgctca
    10801 aacagggcat gatgcgctgg tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc
    10861 tgcttaatca gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct
    10921 acggtcatct accgctggcc tactccgctc gctgctttac cgcgcgttcg gaagaccgcc
    10981 cgaaagatga gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc
    11041 aggaaaacca acaagtgttt gtactcaatg gcattcagac catgagcggc tacgtttaca
    11101 acctcggtaa cgagctgaca tccatgcagg gattggttga tgtggtccgc ctgtcaccgc
    11161 agggtactga cactttcgcg atgctcgacg ccttccgcgc taatgaaaat ggcgctgcgc
    11221 cactattgtt gacggcaaac agcgactgta acggctactg gcggcggctg gcaggactgg
    11281 agctgcaagc ctaagtaaat atgctcgtca tacttcaagt tgcatgtgct gcatctgcat
    11341 tcgttcaccc cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc
    11401 ctgcaactcg aattatttag agcacagctc actttgttaa caactttaac tactctttaa
    11461 tgcagtatta aagattaatc ggtaacaaag tgagctgtta tgactgataa aaccattgcg
    11521 ttttcgttac tcgatctggc ccccattccc gaaggttctt cagcgcgaga agcattctcc
    11581 cactctctcg atctcgcccg tctggctgaa aagcgcggct atcatcgcta ctggctggca
    11641 gaacaccaca atatgactgg cattgccagt gctgccacgt cggtattgat tggctatctg
    11701 gcggcgaata ccaccacgct gcatctgggg tctggcggcg tgatgttgcc taaccactca
    11761 ccgttggtca ttgccgaaca gttcggcacg ctcaatacac tctatccggg gcgaatcgat
    11821 ttggggctgg gtcgtgcgcc aggtagtgac caacggacaa tgatggcgct gcgtcgccat
    11881 atgagcggcg atattgataa tttcccccgc gatgtcgcgg agctggtgga ctggtttgac
    11941 gcccgcgatc ccaatccgca tgtgcgcccg gtaccaggct atggcgagaa aatccccgtg
    12001 tggttgttag gctccagcct ttacagcgcg caactggcgg cgcagcttgg tctgccgttc
    12061 gcgtttgcct cacacttcgc gccggatatg ttgttccagg cgctgcatct ttatcgcagc
    12121 aacttcaaac cgtcggcacg actggaaaaa ccatacgcga tggtgtgcat caatattatc
    12181 gccgccgaca gcaaccgcga tgccgaattc ctgtttacct caatgcagca agcctttgtg
    12241 aagctgcgcc gcggcgaaac cgggcaactg ccgccaccga ttcaaaatat ggatcagttc
    12301 tggtcgccgt ccgaacagta tggtgtgcag caggcgctga gtatgtcgtt ggtaggtgat
    12361 aaagcgaaag tgcgtcatgg cttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt
    12421 atggtcaacg ggcagatttt cgaccaccag gcgcggctgc attcgtttga gctggcgatg
    12481 gatgttaagg aagagttgtt gggatagtgt gtcttaacgc gggaagcctt atccgagctg
    12541 gcaacgctgt cctacgtaga cctgataagc gaagcgcatc aggcattgtg taggcagcag
    12601 aaatgtcgga taaggcaccg ctgattactg atacaccggc agtaaattaa agctcgataa
    12661 aatatgtacc aatgcgttgc cgacgccaaa caccagaatc agcgtaatca tcggcttgcc
    12721 gccccagacg cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg ccagcagtgc
    12781 cggaacaatt gccgcccaga tggtagccgc taaaccagca taaccaatgg cgtacaggaa
    12841 tccattcggg aacagcagac cacccacaac aggcggtgca aaggtcaaca atgctgtttt
    12901 caagcggccc atagccgagt catcgaaacc aaacagatct gccagatagt caaacaaacc
    12961 cagcgttacg ccaaggaacg aactcgctac cgcaaagttt gagaacacga ccagcagaag
    13021 gtccaggcta cggctgttca gcacgccgct taatgcctgt accagcacat caatattacc
    13081 gcccttctct gcaataccga taaactccgg acgcgggatg ttacccatcg tcgccagcaa
    13141 ccagatggta tacagcgcca gtgccatcag cgtaccgtac accaggcatt tcacgatggt
    13201 tttcggatct ttgccgtaat acttcatcag gcttggcacg ttaccgtgat aaccaaacga
    13261 cgccaggcag aacggcaatg tcatcagcag atacggcgca taagatgcat tgctttctgc
    13321 aacgttgaac aatgtcgcag gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa
    13381 ggtaatgact ttcgccccca gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa
    13441 ccacaccaca aacgctacca gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt
    13501 tagcgacatc tctgcgaagg tgtgatgcag aattgaacca ctggcagaaa tataggcata
    13561 ggtcaggata tagagcacaa aggcaatgga aataccgttg accacgttcc agcctttgcc
    13621 cagcaaatct ttggtgatgg tgtcaaaact cgaaccgatt cggtaattca ggttagcttc
    13681 cagaatcatc aagccggaat gcagcataca gaaccaggta aagatcagcg ccgccattga
    13741 ccagaaaaac cacgccccgg acatgaccac tggcagagaa aacatccctg cgccaataat
    13801 ggtgccgccg ataatcacca cgccgccaag cagcgacggt gacgtttggg tggtggttag
    13861 tgttgccatg agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca
    13921 ctgtaccagt acgcgagtac aaaagacaga aaaaaagccc cgatggtaaa aatcggggct
    13981 gtatatatta ttttacagat tgtgttcgct gttcagcgat gattacgcat caccaccgaa
    14041 acgacgacga ccggtagagt catcacgacg cggagcgcgg ccttcacgac gttcgccgct
    14101 aaaacgacga ccatcaccac ggccaccttc acggcgttca ccgctgaagt tacgaccgcc
    14161 ttcacgacgt tcgccaccga aaccacgacc accgccacga cgctcaccgc cagtatgcgg
    14221 ctgtgcatcg cccagtaact gcatgttcat cggcttgttg agaatgcgag tgcgcgtaaa
    14281 gtgttgcagc acttcacccg gcataccttt cggcagttcg atggtggagt gagaagcaaa
    14341 cagcttgatg ttaccaatgt aacggctgct gatgtcgcct tcgttagcaa tcgcaccaac
    14401 gatatgacga acttcaacac catcatcacg gcccacttca atgcggtaca gctgcatatc
    14461 gccaacatca cgacgttcac gacgcggacg atcttcacgg tcaccacgcg ggccacggtc
    14521 gttacgatcg cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg
    14581 cgcatctggc ggtacgatca gagtacgttc accctgtgcc attttcagca gtgccgcagc
    14641 cagagtttcg agatccagct cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc
    14701 gcggtattga tccagatcgc tgctttccag ctgctgctgt actttagcgg cgaatttttc
    14761 cagacggcgt ttgcccagca gttctgcgtt cggcagttct acttccggaa tagtcagctt
    14821 catagtacgt tcaatgttgc gcagcagacg acgctcgcgg ttctcaacga acagcagcgc
    14881 gcggccagca cgacccgcac gaccggtacg accgatacgg tgaacgtaag actcagaatc
    14941 catcgggata tcgtagttaa ctaccaggct gatacgctca acgtccaggc cacgggctgc
    15001 aacgtcggtc gcaatcagga tgtccagacg accatctttc aggcgttcca gtgtctgttc
    15061 acgcagcgcc tggttcatgt caccgttcag cgcggcgctg ttgtagccgt tacgctcaag
    15121 agcttcagcc acttccagag tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa
    15181 atcttccgct tccaggaaac gtaccagtgc ttcgtttttg cgcatacccc agacagtcca
    15241 gtagctctgg ctgatgtcag gacgggtagt cacgctggac tgaatgcgca cttcctgcgg
    15301 ctctttcata aagcggcggg taatgcgacg aatcgcttcc ggcatggttg cagagaacag
    15361 agcggtctga tgaccttccg ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc
    15421 catgcgcagc atttcgtcag cttcatccag aaccagaccg ctcagtttag agaggtccag
    15481 agtgccacgt ttcaggtggt ccagcagacg gcccggagta ccgacaacga tctgcggccc
    15541 ctgacgcagg gcgcgtaatt gcacgtcata acgctggccg ccgtacagag caaccacatt
    15601 tacgccgcgc atgtgtttag agaaatccgt cattgcttca gcaacctgta ccgccagttc
    15661 gcgggtcggt gccagcacca gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa
    15721 cagaggtaaa gagaatgctg cagtttttcc gctccccgtc tgggccatac ccagaacgtc
    15781 gcggccattc agcagatgtg gaatacactc tgcctgaatt ggagatggtt tttcgtaacc
    15841 cagatcgtta agggcttcaa ggataggagc cttcaggccc agatctgcaa aagtggtttc
    15901 gaattcagcc atgtagtacg tgtgcctcaa aattaatggc ggccagtcta cataactcat
    15961 catgaaattg atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta
    16021 acgaacaaca acgccctcac cccttaaggt gatggcaatc aaaaaagatt acgggctgat
    16081 gtgtacgtca gctattgctg gtccgattct gccaggtcat cttggtcctg gcccaggagc
    16141 gataattcca acaatgcgta tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc
    16201 agtttgaaca gtgccgtggc gctgtccgaa tcccccagac ttaggtagta cttacctaaa
    16261 tagaagttgg tttcactgag atgctcagcg agcgaggtgt tatccgttgc gtccgccttg
    16321 agcctttcca ttaacgtttg ttcgctaatg ttgcccaggt agaactcgac aatgttccat
    16381 ccccactgtt ccttatccga tttttcgaag tgctgtttca acacttcttt agcctgcttc
    16441 tcatcgagct tctgctcggc gagataaagc cacagactac ggaaaggatc attgggatcg
    16501 tcttgataaa acgccagcag atcatcttgc gctaacttgt cacgaccgcc gtaatataat
    16561 gcgatcccgc gattcaagtg cgcgtagttg taagttggat caagctcaag tacagaatca
    16621 aacgcttcat aggcagcatc aaaattgcct gcctgcgtta aatatatgcc taagtaattg
    16681 aacacttcag gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat
    16741 gccctcagac cgagactatc atacaacact ccgcgctcat ataaaagctg tgcgcgttcg
    16801 tcatcggtta aagcccgact ggcaaggatt tgttccatac gtgccagaat cacttcctgc
    16861 tgtaaagtcg gttgcaatgg taccgcgagg acttcacttt tacgccagga agtattactg
    16921 catcctgcaa gcgtaagtgc tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc
    16981 cactcccgaa gaccacggtt gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg
    17041 cccggttaaa agccccccgc cgcagcggag ggcaaatggc aaccttactc gccctgttca
    17101 gcagccggag cttccggtgc tgcagcaggt tgagactgct cagtcgcttc tttaatgctc
    17161 agacggatac ggccctggcg atcaacttcc agaactttca ccggtacttc ctgacccatc
    17221 tgcaggtaat cggtcacttt ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga
    17281 ccttctttac cgccgccgat ggcaacaaat gcgccaaagt caacgatacg ggtcacttta
    17341 ccagtgtaga cgcggcccac ttcgatttct gcagtgatct cttcgatacg acgaatagca
    17401 tgtttcgctt tctcgccgtc ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg
    17461 atggtagtgc cagtttcttc ggtcagggca cggataacag agccgccttt accgataacg
    17521 tctttgatct tgtccgggtt gatcttgatg gtatggatac gcggtgcgaa ctcagagata
    17581 tcgccacgcg gcgcgttgat cgcctgttcc attacgccca ggatatgcag acgcgcacct
    17641 ttagcctggt tcagcgcaac ctgcatgatc tctttggtga taccttcaat tttgatatcc
    17701 atctgcaatg cagagatacc ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg
    17761 tgatcttcgt cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc tttcaccaga
    17821 cccattgcga tacccgcaac ggcagctttg atcggcacac ctgcgtccat cagcgccaga
    17881 gacgcgccgc acacggaagc catagaagag gaaccgttgg attcagtgat ttcagacaca
    17941 acacgtacgg tgtacgggaa tttgtccata tccggcatga ctgccagcac gctgcgcttc
    18001 gccagacgac cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct
    18061 acggagtacg gagggaagtt gtagtggaac aggaaggtat cggtacgttc gcccatcagt
    18121 tcatcaagaa cctgcgcgtc acgagcagta cccagcgttg cggtaaccag cgcctgcgtt
    18181 tcaccacggg tgaacagcgc agaaccgtga gtacgcggca gcacgccagt acgcacatcc
    18241 agaccacgga tcatatcttt ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg
    18301 ctacgaacaa cgtttttctc gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg
    18361 gtttcgtctt cagcaagcag cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca
    18421 taacgctctt gtttgtcggt gatgcggtaa gcatcgctca ggcgagcttc agccagtgca
    18481 gcaacgcgcg cgttcagcgc ttcgtttacc ggctccggct gccagtccca acgtggttta
    18541 ccggcttctt tcaccagttc attgatgttc tgaataacaa cctgctgttg ttcatgaccg
    18601 aacactactg cgcccagcat ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc
    18661 agaacagcgg cttcagtacc ggcaacaacc agatccagtt tgctctcttt cagctcgtcc
    18721 tgagtcgggt tcagtacgta ctggtcattg atgtaaccta cgcgagcagc accaatcgga
    18781 ccattgaacg gaataccaga cagagacagt gctgcggaag caccaatcat cgcgacgata
    18841 tccgggttaa cttgcgggtt aacagaaacc acggtggcga taacctgaac ttcgttgacg
    18901 aagccttccg ggaacagcgg gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg
    18961 ccttcgcttg gacgaccttc acgacggaag aagctacccg ggatacgacc agcagcgtag
    19021 gtacgctcct gatagttaac ggtcagtggg aagaagtcct gacctggttt ggcttttttc
    19081 tggccaacaa cggtaacaaa taccgcggtg tcatccatgc taaccataac agcggcagta
    19141 gcctgacgag ccatcatgcc ggtttccaga gtcacggtgt gttggccgta ctggaattta
    19201 cgaacgatcg gattaagcaa tgtaatatcc tttctctttc ttagacagta ccttacggca
    19261 ctggtgttaa tacccgatct tctgcgcatc ctcgcgacta atgacaaccc taacccagct
    19321 ctatgtgggt aaagcctctc attagccgcg cgaacctctg caacggaaga tcattcatag
    19381 caacaataca ttagtttcca gtgaattgct gccgtcagct tgaaaaaagg ggccactcag
    19441 gccccctttt ctgaaactcg gcaggaatta gcgacgcaga cccaggcgct cgatgagctg
    19501 ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc agtttacgac gctgagaaac
    19561 catgcgcagc agaccacgac ggctgtggtg atcttttttg tgctctgcaa agtggccctg
    19621 caggtggttg atctgtgcag tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt
    19681 tgcgtcacga ccaaattcag aaacgatttt agctgttgct tcagtactta gagacatttt
    19741 aaaactccaa agtatataga atgaaaggac gccgatctct aattcagcga tcccagtgta
    19801 cgttacgcaa agtgttaaac aatttacgcg acgttaagcg gcagtattct actcgtagcg
    19861 acttgttatc gcaagacggt taacattacg ccgggtattc aaccaccagg cgacgaggcg
    19921 cgacgcggcc ttcatcgtca atttcgccca taccgataaa cttgccgttc tcaccttctg
    19981 taacgcgaac cagtccttcc agcggcgcac cggatgtacg aactgggtta ccatttttga
    20041 agtaaacaga agacgttaac ggaagattca ccaccgggta gtccgaagct ggactgtcca
    20101 ttggcatcag taatggatca agtaactccg cagccggaat atcctgctgt tcggcttgtt
    20161 cgaccagttc acgcagatgt tccagggtca ccatccgttc aaccggatat ttacttaccg
    20221 ccagacgacg caggtaaata acatgcgcgc cacaaccgag tttttcaccc aggtcatcaa
    20281 tgatggtgcg gatataagtg ccttttgagc agtgaatttc cagctccagc tcattgcctt
    20341 cgtggcgaat aaacagcaat tcataaatgg taatcgggcg cgcttcacgc ggtacttcaa
    20401 tgccctgacg cgcatattcg tacagttttt taccctgata tttcagtgct gaatacatcg
    20461 aagggatctg ttcgatatcg ccacggaaag tatccagcgc cgccgccagt tgctctgcgc
    20521 taaaggttac cggacgttct tcaacgattt gtccgtcggc atcagaagta tcggtacgct
    20581 gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc cagcagatac tgggaaaact
    20641 tcgtcgcttc cccgaagcaa attggcaaca tgccagttgc cagcgggtcc agcgcaccgg
    20701 tatgcccggc acggttggcg ttataaatcc gtttcacttt ttgcagcgca tcattgctgg
    20761 acataccctg cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag
    20821 gacgactcat tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt
    20881 gaccacgctg gtcaccaggt ttgacatgcg catcccttca accagagagt tgtcgtagaa
    20941 gaaggtcagt tccggcacga tacgcaggcg catcgctttc cccagcaggc tgcggatgaa
    21001 accagaagct tcttgcaacg ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt
    21061 gaggaacgtc acatatactt tggcatacgc caggtcgcga gacatttcga caccggaaac
    21121 ggtggtcatc atgcccaggc gaggatcttt aatttcacgc tgcaggatga gagcgatctc
    21181 tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca taataaattc
    21241 tcctgacaaa aaagggactg ttagcccctt tttaaaatta atttcaggtg gaagggctgt
    21301 tcacgttggc ctgataagac gcgccagcgt cacatcaggc aatccatgcc ggatgcggcg
    21361 taaacgcctt atcccgcatg gaaccctaaa aaccttaagc gatggtacgt tggatctcga
    21421 tgatttcgaa tacttcgatc acatcgccag tgcggacgtc gttgtagttc ttaacgccga
    21481 taccacattc cataccgtta cggacttcgt taacgtcatc tttgaagcgg cgcagggact
    21541 ccagctcgcc ttcgtagata accacgttgt cacgcagaac gcggatcggg ttgtgacgtt
    21601 taaccacacc ttcggtaacc atacagcctg cgatggcacc aaatttcggc gatttgaaca
    21661 cgtcacgaac ttccgccaga ccgataatct gctgtttcag ttccggagac agcataccgc
    21721 tcatcgccgc tttcacttcg tcaatcaggt tatagatgac ggagtagtaa cgcagatcta
    21781 ggctttccgc ttcaatcact ttacgtgcag aggcatcagc acgtacgttg aagccaacca
    21841 ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc ggtgatacca cctacgccag
    21901 aaccgatgat cttcacttta acttcgtcag tagacagttt cagcaaggag tcggagatcg
    21961 cttccacaga accctgtacg tctgccttca ggacgatgtt cacttcgtga acttcgcctt
    22021 cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc agtttaactt
    22081 cgcggaattt accctgacga tacagtgcaa cttcacgcgc tttcttctcg tcacgtacaa
    22141 cggtaacttc atcacccgca gccggtacgc cggacaggcc gaggatttcc accggaatgg
    22201 acggacccgc ttccagcact tcctgaccca gttcgttacg catcgcacga acacgaccgt
    22261 attcgaagcc acacagaacg atatcgccct tgtgcagagt accttcacgt accagaacgg
    22321 ttgcaaccgg accacgacct ttatcgagga aggattcgat aaccgcaccg ctcgccatac
    22381 ctttacgtac cgctttcagc tccagaactt ccgcctgcag caggatagcg tccagcagtt
    22441 catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa ctggctttca ccgccccact
    22501 cttctggcag gatgccatac tgggagagtt cgttcttaac acgatccgga tcggcttctg
    22561 gtttatcaat cttgttcact gcaaccacca ccggtacctg cgccgctttc gcgtgctgga
    22621 ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc aacaaccagg actacgatgt
    22681 ccgttgcctg cgcaccacga gcacgcattg aagtaaacgc ggcgtgccct ggggtgtcca
    22741 ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc
    22801 cgcccgcttc gccagaggcc actttcgttg aacgaatgta gtccagcaga gaggttttac
    22861 cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc gcagcccccg
    22921 tgtcacggtc gctcattacc gcctcttcca gctcgttttc acgacgcagg ataactttgt
    22981 ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc gataacctgg ttgatggttg
    23041 ccattgcgcc cagtttcatc atcgctttga tgacctgaga gcctttaacc gccatcttgt
    23101 tcgccagttc gccaacggtg atggtttcgc cgatcacaac gtcacggtta acggcctgag
    23161 ctggcttctg gaagccttgc tgcagggaag aacctttacg ttttccgcct ttacctccac
    23221 gtactgctgc gcgagcttct tcacgatcag cttttgattc agcgtgtttg ttgcctttct
    23281 tcggacgcgc tgctttcgcg ttacgaccac ggccacggcc gccttcgact tcacgatcgc
    23341 tttcgtcttc tgcctggcga gcatgttgag aagtagtgac gtgataatcg ctggaatctt
    23401 cagtcggttc cgcgttatca gtccatttgt tttcttccgc catacgacgt gcttcttcag
    23461 caacgcgacg tgcttcttct tcgagtttac gacgcgcttc ttcttcagct ttacgcttga
    23521 gctctgcagc ttcctgctca cggcgggctt tttcagcctg ggcgttttta gtcatatcgt
    23581 cttgttgatt gctcactttg tctttttccg cagcttcacg tttcgcttgt tcagcagctt
    23641 cacgcttagc ttgttctgcg gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg
    23701 attcttctgc ctcacgacgg gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat
    23761 cgcgtttcac aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac
    23821 cggtaccagg aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc
    23881 ctgaattttt ctgattcagg tggtcaatca aagtctgttt ctcttgtgcg gacacagagt
    23941 cgtcagcaga cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg
    24001 tctgtcgctc tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta
    24061 cagtttatta cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc
    24121 cggctttttc gtcggtcacc ccttcgatat cagccagatc atcaatgccc tgttcggcga
    24181 gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctatcc
    24241 cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct
    24301 gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat
    24361 caaggccttc gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg
    24421 agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga
    24481 aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca
    24541 tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc
    24601 caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat
    24661 ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt
    24721 tatcatccca caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa
    24781 cacgcgcgcc acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt
    24841 tcaccgcgat tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt
    24901 cttcgccgat ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac
    24961 gagtgacgaa cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa
    25021 cgcggtcgcc agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt
    25081 cagcattgtt gcccagctcc agagagatgt tgtcgcggtt tacttttttc accacgccgg
    25141 tgatgatttc accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac
    25201 gcactttctg cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag
    25261 actcaatctg atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg
    25321 cttcaagggt gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga
    25381 aggtgtcaaa atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat
    25441 attttttctt tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag
    25501 gtagcgcctt ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg
    25561 gcttttcacc tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac
    25621 tcagcgcgaa cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg
    25681 ctttgataac gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca
    25741 cctcttctcc gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg
    25801 gtgaggagac ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac
    25861 ttacctggtg gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat
    25921 agatgcgcag tgtggatgtg tgaccgcgaa taaattcgat gccaaccagt tcaaaaccca
    25981 gggcctcaac tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca
    26041 cccccaagac ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa
    26101 ccccgataaa tcggggcttt atataactga accctataat cgcaactgcg gtctggagca
    26161 ctttccagaa ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg
    26221 aaaaaggact ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt
    26281 tatgagcccg acgagctacc aggctgctcc accccgcgcc tgaaactcgg caaattctac
    26341 tcgttttggg taaaagatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa
    26401 atctgcaggc attatagtga tccatgccac attttgtcaa cgtttattgc taatcacgtg
    26461 aatgaatatc cagttcactt tcatttgttg aatacttttg ccttctcctg ctctccctta
    26521 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
    26581 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
    26641 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
    26701 acgtattggt atcgcttttt ctggcggtct ggacaccagt gccgcactgc tgtggatgcg
    26761 acaaaaggga gcggttcctt atgcatatac tgcaaaccta ggccagccag acgaagagga
    26821 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
    26881 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
    26941 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
    27001 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
    27061 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
    27121 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
    27181 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
    27241 cacagactcc aacatgcttg gtgcaacgca tgaagcgaag gatctggaat acctcaactc
    27301 cagcgtcaaa atcgtcaacc cgattatggg cgtgaaattc tgggatgaga gcgtgaagat
    27361 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtggcgc tgaacggtaa
    27421 aacctttagc aacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
    27481 cctgggcatg agcgaccaga ttgagaaccg tatcatcgaa gcgaaaagcc gtggtattta
    27541 cgaagctccg gggatggcac tgctgcacat tgcgtatgaa cgtctgttga ccggtattca
    27601 caacgaagac accattgagc agtatcacgc gcatggtcgt cagttgggcc gtctactgta
    27661 ccaggggcgt tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt
    27721 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
    27781 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
    27841 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat
    27901 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
    27961 cacttcaggc gtgccgcagg tggagaatat ggaaaacaaa ggccagtaat tcgacatcaa
    28021 ccctgccctt cagggggggg gttaccttgc ttatcctctg taaactcctt tctgaacatc
    28081 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
    28141 atcacttcct caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata
    28201 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacgccatcc
    28261 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
    28321 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
    28381 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
    28441 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
    28501 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
    28561 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
    28621 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgca
    28681 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
    28741 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
    28801 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgctgcttat tgcttgatta
    28861 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
    28921 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
    28981 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
    29041 tgatgctctt tcgctgctaa agcaaaatag ttgagattga aaaacggcgt ataggtagca
    29101 aatcgcgacg ctagtatata tggactgaac gtttttttat tttttgcatc tttataagca
    29161 aattgacaag cggaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
    29221 ttttttgtcg gcaaggaaac atcatatttt attattactg cccaaaaaag aagggataaa
    29281 aaagcaaagg cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
    29341 tcactctgga gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
    29401 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
    29461 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
    29521 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
    29581 acacaaagat acaacaacca atgagattta aaacttctag caaatttgtt gaatactgtc
    29641 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
    29701 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
    29761 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
    29821 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
    29881 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
    29941 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
    30001 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
    30061 aacgaaaaaa gacgcttttc agcgtctctt ttctggagtt tggtaccgag gacgggactt
    30121 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
    30181 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
    30241 tggttgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
    30301 ggttttgttg ctgttgatgt tacccagcac cagactgatg atgaagaata acgttgccag
    30361 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
    30421 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
    30481 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
    30541 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
    30601 tctttcccac tgaagcgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
    30661 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
    30721 aaggcgacaa tcgccgcctt tttagccagt catttatcgt tttaaacggc ttttactgca
    30781 tcggcgatgc ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc caccatcacg
    30841 cgaattaacg gttcggtgcc ggatttacgc agcaacacgc gtccacggtt gcccagcgca
    30901 gcttcaacct ctgcggtcac ggctttaact gactcatgct caagcggatc gccgctacct
    30961 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
    31021 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
    31081 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
    31141 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
    31201 ggaataccta actgtttcag cgccagctca agccccatgt tactcatcaa tgtacccaca
    31261 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
    31321 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
    31381 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
    31441 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
    31501 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
    31561 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
    31621 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
    31681 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
    31741 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
    31801 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gatacgccac ggccggtgtt
    31861 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
    31921 gactccagca tatagccaga aatacgcgtg tctttactaa taatgatctt acgggagccg
    31981 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
    32041 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
    32101 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
    32161 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
    32221 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
    32281 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
    32341 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
    32401 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
    32461 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
    32521 atgcatcaga caaaccggta aaccggtttc tgccgccgcc tccagagcgc caggttcgga
    32581 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
    32641 tggtttggat gtatctaccg agagccagac ttcgaagcgt tgagcaattg cctcaaccac
    32701 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
    32761 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
    32821 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
    32881 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
    32941 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
    33001 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
    33061 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
    33121 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
    33181 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
    33241 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
    33301 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
    33361 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
    33421 ggacccaatt tctcagagaa gccccactga gtcaccatat tacgcgccag gttggtcgca
    33481 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
    33541 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
    33601 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
    33661 gtcactttgt gcaccggatc gtgttccggc accaagcgac cgataatcgc atgacccgct
    33721 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
    33781 cccatcatga ttttatcttt cgctttttcg aactcaatca tcgacacaac gcgtttgttg
    33841 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
    33901 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
    33961 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
    34021 cggtcgaaac ggccaggacg cagcagggct gggtcaagaa cgtccggacg gttagtcgcg
    34081 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
    34141 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
    34201 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
    34261 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
    34321 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcaacgt tttaccggta
    34381 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
    34441 ctcggctcgc gcagatactc taccagttca gcaacttctt cttttgcttc gtcgcagccc
    34501 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
    34561 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccag
    34621 acaccaatca gcaacagcat cgggaaccaa gagatgaaga tagaagccag caggcttggt
    34681 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
    34741 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
    34801 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
    34861 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
    34921 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
    34981 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
    35041 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
    35101 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
    35161 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
    35221 cctggcgcta atacatcacg acacatttct aacgccagtt ccaccagata catggcacgg
    35281 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
    35341 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
    35401 ccctgaagaa agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg
    35461 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
    35521 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
    35581 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
    35641 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
    35701 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
    35761 atatgggaga tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat
    35821 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
    35881 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
    35941 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
    36001 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
    36061 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
    36121 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
    36181 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
    36241 atcaacatct tgatgtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
    36301 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
    36361 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
    36421 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
    36481 caaaaataac gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca
    36541 gcttggcttc gatgtcttta atacggcctt cgcagaaacc ctgctgttca cgagctgcgt
    36601 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    36661 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    36721 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    36781 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    36841 aaaaaatacc gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat
    36901 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    36961 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    37021 tgaccagctg tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac gttactcaac
    37081 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    37141 ttgattacca cagtcagcag atggcgctgc ccgccagtac gcagaaagtg attactgcgc
    37201 tggcggcgtt gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag
    37261 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    37321 cgttaaaacg tcaggatatt cgcaatatga tcgcgacttt gaaaaaatct ggcgtcaacc
    37381 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    37441 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgct gccatagttg
    37501 accgcaactg tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta
    37561 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgag
    37621 gttctgccga agcgcaatac tgcgaactgg atgtcatacc aggcgacctg aaccgcttta
    37681 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    37741 atggagccag ctatgccggg gcaattctga aagatgagtt aaaacaggca ggtatcacct
    37801 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    37861 aacagtcggc cccgttgcac gatctgctta agattatgct gaaaaagtcg gacaacatga
    37921 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc
    37981 gggccgggtc ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    38041 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    38101 tgatgcaggt gttgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    38161 tgccgctggc gggctatgat ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    38221 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    38281 tcattaccac agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag
    38341 tagaaccggc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    38401 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt
    38461 gctgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcatca
    38521 tcccagtcct catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc
    38581 cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca
    38641 atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag
    38701 atcagataat atttatcttc ccagcccagc gcttcagcaa tcgctttcgc tttctcttca
    38761 gcttctgcct tatccagcag atcgatctta ttgaacacta accaacgcgg tttcgccgcc
    38821 agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg
    38881 gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc
    38941 ttcaggaagc ggatacccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata
    39001 tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga
    39061 gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcgcg aataaaggtc
    39121 gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc
    39181 agcagctcac gcttatcacc cggcgtaccg ttggttttct gccgtggtgt acggttaacg
    39241 gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc
    39301 tgaccgtgtt tagtcatatc gcccatggtt tcaccagtac cctggtcgat tacacgcgta
    39361 cctaccggca ctttaatcgt cacgtcttta ccgcgcttac cggtacaatc gcggcttgcg
    39421 ccgttctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc
    39481 aggttttcgt cggcttccat ccatacgtca ccaccatcac caccgtcgcc gccatccggg
    39541 ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct
    39601 gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg
    39661 cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga
    39721 ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg
    39781 tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac
    39841 agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc
    39901 atcgcttccg ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacat
    39961 gcgagctgcc agtggctaag ctgcgctatc actcctggct ttgccagagg gaagagcgca
    40021 attgtacata aagtgtacag caaaaacagg atctgcggtg aggccagccg acgcaataaa
    40081 accttttgcg ccacgccata actcacccaa accgtcgccg caccgacccc aaagatgact
    40141 ccccaggtgt aatcagtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc
    40201 accaggccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc
    40261 aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa
    40321 gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg
    40381 gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc
    40441 aacctcttct tcaccgcaag aatggcaccc aggccaatac tcgccatcaa gaaacggtaa
    40501 aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc
    40561 ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata
    40621 ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgctcat ttttaccggt
    40681 tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga
    40741 aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat
    40801 ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg
    40861 ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg
    40921 ccaccgaaac gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag
    40981 ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag
    41041 taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac
    41101 gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga
    41161 ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca
    41221 gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg
    41281 tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa
    41341 actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag
    41401 ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa
    41461 aaaaaacaca acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcacccta
    41521 tattcctaac cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag
    41581 cccggctttt gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg
    41641 tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc caactgatca atcagttagg
    41701 ctattacatc gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc
    41761 acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat
    41821 ccacacggcg actctgctac acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa
    41881 agctaccgcc aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta
    41941 tacccgcgcg ttccagatga tgaccagcct cggttcactc aaagtgctgg aagtcatgtc
    42001 agaagctgta aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc
    42061 ggacatcact gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga
    42121 ggctgccgcg cagtgttccg ggattctggc tggctgtacg ccggaggagg agaaaggcct
    42181 gcaggattat gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta
    42241 caatgccgat ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc
    42301 gacgctgccg ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg
    42361 taccgccatc gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa
    42421 cgcttgtgga tctcttgaat ggacgcgtca gcgcgccgag gaagaagcag acaaagccat
    42481 cgcagcgtta caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca
    42541 catcgctgtt caacgcgatc gttaatcccc tcccttcatc ccgcgcagtg cgcgggatga
    42601 ttccagtaaa ttccataaaa acactaattc agcgctataa cacctgaaaa aaaagggcga
    42661 tgaatcttta tatattctga atattcacac tctttacagg aactttttag agcaataggc
    42721 catcaggagt atagtgatgc ccgacagaag aagtgttctg aatgaaagcg actaacttaa
    42781 ggagtgagga aagatggaaa gtaatttcat tgactggcat ccccctgaca tcattgcagg
    42841 tttgcgcaag aaaggaacgt caatggcggc ggaatctcgc agaaatggtt tgagttcctc
    42901 aacgctggca aacgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    42961 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    43021 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    43081 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctt
    43141 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    43201 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    43261 tgatgcacgc agatcggttg ccataacctg tgcaccagaa agtttttcaa caccatgaca
    43321 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    43381 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    43441 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    43501 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    43561 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    43621 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    43681 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    43741 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    43801 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    43861 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    43921 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    43981 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    44041 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    44101 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    44161 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctgactta gcagcttcat
    44221 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    44281 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    44341 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    44401 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    44461 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    44521 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    44581 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    44641 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    44701 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    44761 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    44821 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    44881 caatttcgcc agggtataca ctttgtcgtt taccccctga agcgtcacat tgttgccctg
    44941 ctttttcgcc agatcgataa gctggagcag cagtgccagt ccccccgtat ccacgcggga
    45001 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    45061 cggtagcaaa acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    45121 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    45181 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    45241 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttg
    45301 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcgggcg gccattcgga
    45361 tcaataatgg taacgcgaat cggcacaatg gttttatcgc ccagcggctg ttctggcgca
    45421 atctgatagg tttgaccatg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    45481 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    45541 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    45601 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    45661 aacgttttct gcgccgcctc gtccatcagc ttataaggat tggtctggtc tgccgcggtt
    45721 gccgcactca gcggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    45781 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    45841 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    45901 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    45961 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    46021 tcccccagta ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    46081 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    46141 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    46201 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    46261 gccgccttca ggcaaacaaa cagagccgcc agcagtgctg ctaataaaaa gatacccacc
    46321 caaatttcat tttttttcgt ttgcatgtac tcaattccca aacatcaatg cggtcagcac
    46381 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    46441 aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt
    46501 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    46561 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    46621 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    46681 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    46741 actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    46801 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    46861 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    46921 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    46981 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    47041 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    47101 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    47161 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    47221 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    47281 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    47341 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    47401 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    47461 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccggaaagtt
    47521 cagaaggcat tagtttagcc gctccacgca gccccacggc ctccagcttc atcatcaccg
    47581 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    47641 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    47701 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    47761 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    47821 ccgatggccc catgatcgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    47881 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    47941 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    48001 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    48061 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    48121 tcagcggacc ggcgagtata cctgaagaaa ggacgttaga tgcttttagc tacggcactg
    48181 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    48241 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    48301 ggtacatcgt taccagaaat catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    48361 ttagccgttg gtacagccct cggctcaaac attatcaata ttttgctgat cctcggtctg
    48421 gctgcgctgg ttcgtccttt taccgtccat tccgatgttc tgcgtcgtga attaccctta
    48481 atgttgttgg tcagcgtagt ggccggttcc gtactctatg acggacaact tagtcgcagc
    48541 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    48601 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    48661 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    48721 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    48781 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    48841 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    48901 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacacc aggagagatt
    48961 gatccattgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    49021 ttgctgtgct ggcggcgctc cccgcaaccg ggccgtggtg taggggtatt attaactggc
    49081 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    49141 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    49201 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    49261 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    49321 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    49381 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    49441 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    49501 agtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    49561 ggcgcgcgcc gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    49621 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    49681 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    49741 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    49801 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    49861 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    49921 tttacgccgt gtcttcgata tgggagtgga tgttcgtcag ttaagtattg ccgatgtgat
    49981 gacgccgggg ggaatacgcg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    50041 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    50101 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    50161 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatagca
    50221 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    50281 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    50341 attcgttgtg cgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    50401 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    50461 aaactgatcg cctttagcga actgctggaa aaactggcga ttgccccgga aaatgtggct
    50521 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    50581 gtggccgatg ctcatccact gttgatcccg cgcgccgatt atgtgacgcg cattgctggc
    50641 ggtcgtggcg cagtgcgaga agtttgcgac ttattactcc tggcgcaggg caaactggat
    50701 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttaccatt gtgctatcac
    50761 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    50821 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    50881 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    50941 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    51001 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    51061 acgttgaagt caacgcactt gtgccggact ctcaacttcg cagaatcacg acggataacg
    51121 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    51181 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    51241 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    51301 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    51361 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    51421 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    51481 gtcgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    51541 gcaacgttct accagatgca ggacaacggt aaacccgttg aaggtcatgc ttcccagatg
    51601 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtc
    51661 gatagcaaca ttaagggcga taagatcact tacctggtga aagagcagaa aatgcaggct
    51721 ttcagcgaca aaggcaagcg cgtaacaacc gttctggtgc cgtcgcagct gcaggacaaa
    51781 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    51841 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    51901 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    51961 actttctata tggttgttgg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    52021 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    52081 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    52141 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    52201 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    52261 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctact cgacgaaccg
    52321 tttgccgggg ttgacccgat ctcggttatc gacattaaac gcatcattga acacctgcgc
    52381 gacagcggcc tgggcgtact gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    52441 gaacgcgctt atatcgtcag tcaggggcat ttgattgccc acggcacgcc tacagaaatc
    52501 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    52561 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    52621 tcaggcttag ccaacaactg gcgatgacgc cacagcttca acaggcgatt cgtctgttgc
    52681 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    52741 ttgagcaaat cgacactcat gaagaaatcg acaccagcga aacgcaagac agtgaaacgc
    52801 tggacaccgc cgacgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    52861 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    52921 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    52981 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    53041 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    53101 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    53161 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    53221 ataaaaccac gccgtggctg gaagaggcca gactgatcat tagcgatcat ctcgatctgt
    53281 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    53341 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    53401 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacgg
    53461 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    53521 gcaataacgc gcgcaacgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    53581 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    53641 tcgttgaaca gcagcaagcc ttctttgagc aaggtgaaga atatatgaaa ccgatggtac
    53701 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    53761 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    53821 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    53881 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    53941 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    54001 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    54061 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaga
    54121 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    54181 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    54241 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    54301 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    54361 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    54421 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    54481 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt tcactgtcag
    54541 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    54601 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    54661 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    54721 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    54781 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    54841 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    54901 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    54961 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    55021 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    55081 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaaccct ggccgatcgc
    55141 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    55201 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactcctgtt cctggatgcc
    55261 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    55321 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    55381 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    55441 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    55501 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    55561 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    55621 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgag
    55681 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    55741 accggcggga agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    55801 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    55861 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    55921 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    55981 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    56041 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    56101 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    56161 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    56221 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagaggcatt gtttaggttt
    56281 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    56341 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    56401 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    56461 agatccgccg tgagttaaag tactacgcgt atcttggtcg ccggttaaaa gactggagtg
    56521 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    56581 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    56641 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    56701 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccacatca
    56761 gtgagtcaga ggttatcccg gtcgtctgcg acaacattgg tgaaagcaca ttgattccac
    56821 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    56881 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    56941 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    57001 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    57061 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    57121 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa
    57181 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    57241 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    57301 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    57361 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    57421 ggtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cactgcgaga
    57481 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    57541 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    57601 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    57661 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    57721 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    57781 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    57841 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    57901 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    57961 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    58021 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    58081 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    58141 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    58201 aacatcaacc tgctgcttat caggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    58261 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    58321 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    58381 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    58441 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    58501 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    58561 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    58621 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    58681 gaaggtcagg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    58741 ccgccgcacc tttaatttta tgtccttcct caacaatgcc ttttttatcc tgcgccgtca
    58801 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    58861 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    58921 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    58981 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    59041 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    59101 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    59161 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    59221 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    59281 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    59341 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    59401 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    59461 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    59521 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    59581 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    59641 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    59701 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    59761 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    59821 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    59881 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    59941 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atgcggctca
    60001 gaccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    60061 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    60121 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    60181 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    60241 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    60301 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    60361 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    60421 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    60481 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    60541 tttcacgaac ggcaatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    60601 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    60661 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    60721 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    60781 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    60841 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    60901 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    60961 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    61021 tagccacgga cgtcccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    61081 ctcgaccatt cccgtccatc gattttcgca ccacagcgga gcaagcagcg tcgggcgacg
    61141 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    61201 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    61261 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    61321 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcaccag gcaggccgac
    61381 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    61441 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    61501 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    61561 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    61621 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tagtatacgc
    61681 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    61741 ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    61801 gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt
    61861 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    61921 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    61981 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    62041 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    62101 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgtcatta
    62161 ccaattaagg cagtataaaa tgctggtttt ctcgtcagtt caaggtagga taagggttaa
    62221 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    62281 agataaaatt cattttaatt tcagttactt aataaagaat tttgcgctaa agcacatttc
    62341 tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct
    62401 ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct
    62461 tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatacgaggc gcgcgtatga
    62521 cacgcaaccc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg gaagtggggt
    62581 tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata
    62641 actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta
    62701 ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa
    62761 ccggtgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc
    62821 aggagcgcgg ctggcgttta gcaaaaaact acgctgtcgg gatgctcttc ctgaataaag
    62881 atcctgaact cgcggctgcc gcacgccgca tcgttgaaga agagctgcaa cgcgaaacct
    62941 tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc
    63001 tctcctctct gccccgcatt gagcaaattt tcgttaacgc tccggcaggc tggcgtccgc
    63061 gggatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg
    63121 acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa ggtctgtgta
    63181 tgccggcgga tctgccgcgc ttttatctgg atcttgcgga cctgcgtctg gaatcggcca
    63241 tctgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg ctggcgcagc
    63301 cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac cgccaatggg
    63361 cgcgtgcgcg tacctataaa ttccagacgc cgcttatccc tgatctgcac gacgccgcac
    63421 cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactgctgc
    63481 tggcaggcgg gatggatatc atccgcgcca tgcgtctgtt agtgccaccc gcctggcaga
    63541 acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg
    63601 agccgtggga tggcccggcg ggcatcgtga tgtccgacgg tcgttttgcc gcctgtaacc
    63661 tcgaccgtaa cggtctgcgt ccggcgcgct acgtcatcac caaagataag ctcatcacct
    63721 gcgcctctga agtcggtatc tgggattacc agcctgacga agtggtcgaa aaaggccgcg
    63781 tcgggccagg cgaactgatg gttatcgaca cccgcagtgg gcgtattctg cactcggcag
    63841 aaaccgatga cgatctgaaa agccgccatc catataaaga gtggatggag aaaaacgtcc
    63901 gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaattggacg
    63961 acgacacgct tgccagctac cagaaacagt ttaactacag tgcggaagag ctggactccg
    64021 taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc gatgataccc
    64081 cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc cagcagtttg
    64141 cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca
    64201 ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg caccgtttaa
    64261 gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg atgaaagagg
    64321 agcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag
    64381 cgacagtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc accgtgctgc
    64441 tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg
    64501 ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg
    64561 tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggc ttcggcgcga
    64621 cggctattta tccatacctt gcctatgaaa cgctgggccg cctggttgac acccatgcga
    64681 ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac aaaggcttgt
    64741 acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt
    64801 ttgaagcggt cggtctgcac gatgatgtag tgggcctgtg cttccagggg gcggtcagcc
    64861 gcattggtgg ggcgagcttt gaagacttcc agcaggatct gctgaacctg tcgaaacgtg
    64921 cctggctggc gcgtaagccc atcagccagg gcggcctgct gaaatacgtc cacggcggcg
    64981 aataccacgc ttacaacccg gacgtggtgc gcacgctgca acaggcggta caaagcggcg
    65041 agtacagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctgc
    65101 gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg
    65161 caagcgaact gtttaaacgc tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg
    65221 aagcccacga ggcgctggcg gaagcgatga atagcatcgg cggtaactcg aactccggtg
    65281 aaggcggcga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg
    65341 cttctggtcg cttcggcgtt actccggcgt atctggtcaa tgccgacgtc attcagatta
    65401 aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc
    65461 cttacatcgc caaactgcgc tattcggtgc ccggagtgac gctgatctcc ccgccgccgc
    65521 accacgatat ctactctatc gaggacttag cgcagctcat tttcgacctc aagcaggtta
    65581 acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga
    65641 ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggtggcaccg
    65701 gcgcaagccc gctgtcgtca gtgaaatacg caggttgccc gtgggagctg gggcttgttg
    65761 aaacccagca ggcgctggtt gccaatggtc tgcgtcataa gatccgtttg caggtcgatg
    65821 gcggcctgaa aaccggtgtt gatatcatca aagcggcgat tctcggcgca gaaagcttcg
    65881 gcttcggcac tggcccaatg gtcgcgctcg gctgtaaata tctgcgtatt tgccacctga
    65941 acaactgcgc aacgggtgtg gcaactcagg atgacaaact gcgtaagaat cactatcacg
    66001 gcctgccgtt caaggtgacg aattactttg agtttatcgc ccgtgaaacc cgcgagctga
    66061 tggcacagct gggggtaacg cggctggtgg atctgattgg tcgcaccgac ctgctgaaag
    66121 agctggacgg tttcaccgcc aaacagcaga aactggcgct gtcgaagctg ctggagaccg
    66181 ccgaaccgca tccaggtaag gcactctact gcaccgaaaa caacccgccg tttgataacg
    66241 gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca
    66301 aaaccttctg gttcgatatc cgcaataccg atcgctccgt gggcgcgtcg ctttcaggct
    66361 atatcgccca gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca
    66421 acggcaccgc aggccagagt ttcggtgtat ggaacgcggg cggcgtggaa ctgtacctga
    66481 ccggggatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc gccattcgtc
    66541 ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac acctgcctgt
    66601 atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc
    66661 ggaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga
    66721 cgggcggtat tgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg
    66781 gcggcttcgc ttacgttctc gatgaaagcg gtgatttccg caaacgcgtt aacccggaac
    66841 tggtcgaggt cttaaacgtt gacgatctgg cgatccatga agagcatctg cgcggtctta
    66901 tcaccgagca tgttcagcat actggctctc agcgcggtga agagattctg gcgaactggt
    66961 caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc
    67021 tgggtcaccg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa
    67081 caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg cctaagaaac
    67141 cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gcagttttcc gaaggccagg
    67201 ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc
    67261 cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt atttttgaag
    67321 cggcggaact gtcgcaccag accaacaccc tgccggaagt ttgcggacga gtctgcccgc
    67381 aagaccgtct gtgcgaaggt tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg
    67441 gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt ccggatatgt
    67501 ccggcgtgaa gcaaaccggt aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg
    67561 cgtgtgcgga tgtcctgacg cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag
    67621 aaattggcgg gctgctgacc ttcggtattc cggccttcaa gctggaaaaa gaggtaatga
    67681 cgcgtcgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg
    67741 gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtcttc cttggcgtcg
    67801 ggacttatca gtcaatgcgc ggcggtctgg aaaacaaaga cgccgatggc gtgtacgcag
    67861 cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg
    67921 aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga
    67981 tggactgcgt gcgtacgtcc gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc
    68041 gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg
    68101 tagagttcaa attcaacgtc cagccactgg ggattgaagt gaacggtaac ggcaaagtca
    68161 gcggcgtaaa aatggtacgt accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg
    68221 cggagatcgt tgcaggttcc gaacatatcg ttccggcaga tgcagtgatc atggcgtttg
    68281 gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcacaag
    68341 gccgcatcat cgccccggaa ggcagcgaca acgccttcca gaccagcaac ccgaaaatct
    68401 ttgctggcgg cgatatcgtc cgtggttccg atctggtggt aaccgctatt gccgaaggtc
    68461 gtaaggcggc agacggtatt atgaactggc tggaagttta agctctgtcc cagcgccgcc
    68521 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    68581 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    68641 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    68701 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcacctg gtcgatagcg
    68761 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    68821 gccgacgaat tgtcgaattt gaacaaggtg gcgaggcaag ggctgcgtat ggtgcgcagc
    68881 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    68941 acttacgcca aatgaggctt tttt
//
LOCUS       DAJVTY010000004        66347 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVTY010000004 DAJVTY010000000
VERSION     DAJVTY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66347
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.004"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OQQ66_000255"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OQQ66_000255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1903308.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQQ66_000256"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQQ66_000256"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1903309.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQQ66_000257"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQQ66_000257"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1903310.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQQ66_000258"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQQ66_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1903311.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQQ66_000259"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQQ66_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1903312.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQQ66_000260"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQQ66_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1903313.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OQQ66_000261"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OQQ66_000261"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1903314.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10128
                     /gene="mrcB"
                     /locus_tag="OQQ66_000262"
     CDS             7606..10128
                     /gene="mrcB"
                     /locus_tag="OQQ66_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1903315.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10348..12537
                     /gene="fhuA"
                     /locus_tag="OQQ66_000263"
     CDS             10348..12537
                     /gene="fhuA"
                     /locus_tag="OQQ66_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1903316.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12588..13385
                     /gene="fhuC"
                     /locus_tag="OQQ66_000264"
     CDS             12588..13385
                     /gene="fhuC"
                     /locus_tag="OQQ66_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1903317.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13385..14275
                     /gene="fhuD"
                     /locus_tag="OQQ66_000265"
     CDS             13385..14275
                     /gene="fhuD"
                     /locus_tag="OQQ66_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1903318.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14272..16254
                     /gene="fhuB"
                     /locus_tag="OQQ66_000266"
     CDS             14272..16254
                     /gene="fhuB"
                     /locus_tag="OQQ66_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1903319.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="OQQ66_000267"
     CDS             complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="OQQ66_000267"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1903320.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAAIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17794..19215
                     /gene="clcA"
                     /locus_tag="OQQ66_000268"
     CDS             17794..19215
                     /gene="clcA"
                     /locus_tag="OQQ66_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1903321.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMEALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19239..19304
                     /locus_tag="OQQ66_000269"
     CDS             19239..19304
                     /locus_tag="OQQ66_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903322.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19297..19641
                     /gene="erpA"
                     /locus_tag="OQQ66_000270"
     CDS             19297..19641
                     /gene="erpA"
                     /locus_tag="OQQ66_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1903323.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19688..20311)
                     /locus_tag="OQQ66_000271"
     CDS             complement(19688..20311)
                     /locus_tag="OQQ66_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1903324.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20349..21148)
                     /gene="btuF"
                     /locus_tag="OQQ66_000272"
                     /pseudo
     CDS             complement(20349..21148)
                     /gene="btuF"
                     /locus_tag="OQQ66_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="OQQ66_000273"
     CDS             complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="OQQ66_000273"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1903325.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHGKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21923..23440
                     /gene="dgt"
                     /locus_tag="OQQ66_000274"
     CDS             21923..23440
                     /gene="dgt"
                     /locus_tag="OQQ66_000274"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1903326.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23570..24994
                     /gene="degP"
                     /locus_tag="OQQ66_000275"
     CDS             23570..24994
                     /gene="degP"
                     /locus_tag="OQQ66_000275"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1903327.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25149..26306
                     /gene="cdaR"
                     /locus_tag="OQQ66_000276"
     CDS             25149..26306
                     /gene="cdaR"
                     /locus_tag="OQQ66_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS1903328.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26395..26781)
                     /locus_tag="OQQ66_000277"
     CDS             complement(26395..26781)
                     /locus_tag="OQQ66_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1903329.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="OQQ66_000278"
     CDS             complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="OQQ66_000278"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS1903330.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="OQQ66_000279"
     CDS             complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="OQQ66_000279"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1903331.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="OQQ66_000280"
     CDS             complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="OQQ66_000280"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1903332.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31399..32193)
                     /gene="map"
                     /locus_tag="OQQ66_000281"
     CDS             complement(31399..32193)
                     /gene="map"
                     /locus_tag="OQQ66_000281"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1903333.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32561..33286
                     /gene="rpsB"
                     /locus_tag="OQQ66_000282"
     CDS             32561..33286
                     /gene="rpsB"
                     /locus_tag="OQQ66_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1903334.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33544..34395
                     /gene="tsf"
                     /locus_tag="OQQ66_000283"
     CDS             33544..34395
                     /gene="tsf"
                     /locus_tag="OQQ66_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1903335.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34542..35267
                     /gene="pyrH"
                     /locus_tag="OQQ66_000284"
     CDS             34542..35267
                     /gene="pyrH"
                     /locus_tag="OQQ66_000284"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1903336.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35559..36116
                     /gene="frr"
                     /locus_tag="OQQ66_000285"
     CDS             35559..36116
                     /gene="frr"
                     /locus_tag="OQQ66_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1903337.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36208..37404
                     /gene="ispC"
                     /locus_tag="OQQ66_000286"
     CDS             36208..37404
                     /gene="ispC"
                     /locus_tag="OQQ66_000286"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1903338.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37592..38368
                     /gene="ispU"
                     /locus_tag="OQQ66_000287"
                     /gene_synonym="uppS"
     CDS             37592..38368
                     /gene="ispU"
                     /locus_tag="OQQ66_000287"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1903339.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTGPVMKQPDGGRFC"
     gene            38362..39219
                     /gene="cdsA"
                     /locus_tag="OQQ66_000288"
     CDS             38362..39219
                     /gene="cdsA"
                     /locus_tag="OQQ66_000288"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1903340.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39231..40583
                     /gene="rseP"
                     /locus_tag="OQQ66_000289"
     CDS             39231..40583
                     /gene="rseP"
                     /locus_tag="OQQ66_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1903341.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSLLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40613..43045
                     /gene="bamA"
                     /locus_tag="OQQ66_000290"
     CDS             40613..43045
                     /gene="bamA"
                     /locus_tag="OQQ66_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1903342.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43167..43652
                     /gene="skp"
                     /locus_tag="OQQ66_000291"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43167..43652
                     /gene="skp"
                     /locus_tag="OQQ66_000291"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1903343.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43656..44681
                     /gene="lpxD"
                     /locus_tag="OQQ66_000292"
     CDS             43656..44681
                     /gene="lpxD"
                     /locus_tag="OQQ66_000292"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1903344.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44786..45241
                     /gene="fabZ"
                     /locus_tag="OQQ66_000293"
     CDS             44786..45241
                     /gene="fabZ"
                     /locus_tag="OQQ66_000293"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1903345.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45245..46033
                     /gene="lpxA"
                     /locus_tag="OQQ66_000294"
     CDS             45245..46033
                     /gene="lpxA"
                     /locus_tag="OQQ66_000294"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1903346.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46033..47181
                     /gene="lpxB"
                     /locus_tag="OQQ66_000295"
     CDS             46033..47181
                     /gene="lpxB"
                     /locus_tag="OQQ66_000295"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1903347.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLVKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47178..47774
                     /gene="rnhB"
                     /locus_tag="OQQ66_000296"
     CDS             47178..47774
                     /gene="rnhB"
                     /locus_tag="OQQ66_000296"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1903348.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMVVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47811..51293
                     /gene="dnaE"
                     /locus_tag="OQQ66_000297"
     CDS             47811..51293
                     /gene="dnaE"
                     /locus_tag="OQQ66_000297"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1903349.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51306..52265
                     /gene="accA"
                     /locus_tag="OQQ66_000298"
     CDS             51306..52265
                     /gene="accA"
                     /locus_tag="OQQ66_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1903350.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSSYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52364..54473
                     /gene="ldcC"
                     /locus_tag="OQQ66_000299"
                     /pseudo
     CDS             52364..54473
                     /gene="ldcC"
                     /locus_tag="OQQ66_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54530..54919
                     /locus_tag="OQQ66_000300"
     CDS             54530..54919
                     /locus_tag="OQQ66_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1903351.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54984..56282
                     /gene="tilS"
                     /locus_tag="OQQ66_000301"
     CDS             54984..56282
                     /gene="tilS"
                     /locus_tag="OQQ66_000301"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1903352.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56331..56591)
                     /gene="rof"
                     /locus_tag="OQQ66_000302"
     CDS             complement(56331..56591)
                     /gene="rof"
                     /locus_tag="OQQ66_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1903353.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56578..56778)
                     /locus_tag="OQQ66_000303"
     CDS             complement(56578..56778)
                     /locus_tag="OQQ66_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1903354.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYINE"
     gene            56944..57489
                     /locus_tag="OQQ66_000304"
     CDS             56944..57489
                     /locus_tag="OQQ66_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1903355.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57486..57908
                     /gene="arfB"
                     /locus_tag="OQQ66_000305"
     CDS             57486..57908
                     /gene="arfB"
                     /locus_tag="OQQ66_000305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1903356.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57922..58632
                     /gene="nlpE"
                     /locus_tag="OQQ66_000306"
                     /gene_synonym="cutF"
     CDS             57922..58632
                     /gene="nlpE"
                     /locus_tag="OQQ66_000306"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1903357.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58832..59652)
                     /locus_tag="OQQ66_000307"
                     /pseudo
     CDS             complement(58832..59652)
                     /locus_tag="OQQ66_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59705..61423)
                     /gene="proS"
                     /locus_tag="OQQ66_000308"
     CDS             complement(59705..61423)
                     /gene="proS"
                     /locus_tag="OQQ66_000308"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1903358.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61533..62240)
                     /gene="tsaA"
                     /locus_tag="OQQ66_000309"
     CDS             complement(61533..62240)
                     /gene="tsaA"
                     /locus_tag="OQQ66_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1903359.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62237..62641)
                     /gene="rcsF"
                     /locus_tag="OQQ66_000310"
     CDS             complement(62237..62641)
                     /gene="rcsF"
                     /locus_tag="OQQ66_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1903360.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62759..63574)
                     /gene="metQ"
                     /locus_tag="OQQ66_000311"
     CDS             complement(62759..63574)
                     /gene="metQ"
                     /locus_tag="OQQ66_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1903361.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63614..64267)
                     /gene="metI"
                     /locus_tag="OQQ66_000312"
     CDS             complement(63614..64267)
                     /gene="metI"
                     /locus_tag="OQQ66_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1903362.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64260..65291)
                     /gene="metN"
                     /locus_tag="OQQ66_000313"
     CDS             complement(64260..65291)
                     /gene="metN"
                     /locus_tag="OQQ66_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1903363.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65479..66051
                     /gene="gmhB"
                     /locus_tag="OQQ66_000314"
     CDS             65479..66051
                     /gene="gmhB"
                     /locus_tag="OQQ66_000314"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1903364.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15761 a        16431 c        18205 g        15950 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taattcgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgatcctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgagcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtgga
      481 gtgcggtaaa acgaagaaac ggcaagaatg tggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgc ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac
      661 gcggtgacgg ctcttcatcc aactcgttaa gcatcccttt ttgatctggt ggcgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcgttacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt catgccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtt gcaacttcga taatctccgg gccaaacatc acatgagcca
     1681 gacggaaacg gcgacccaca aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttattgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcgtccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctacctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgccgcg
     2521 cagctggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgacaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgttcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttctgc
     4021 gccatatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttagtt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc cacgcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaagggg agagaattcc attgcgacaa tccttgttat
     4381 tgcattagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacctggcgg cgggattgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggcggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctcggcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtttta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggaggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtagttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggagt tgagcggtgt tggattggtg
     5341 atccttgatg aatttcatga gcgtagcttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctaa ttatgtcggc tacgctggac
     5461 aacgaccgct tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgatgccgtt
     5581 gcggtagcca ccgctgaaat gctgcgtcag gaaagcggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg tgtgcaggaa caactggctt cgcgcatcgg cagtgatgta
     5701 ttgctctgcc cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ttttgatccg
     5881 cgcacggggc taacgcgact gattactcaa cgcgttagcc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atctgcctgc atctaatcgc caaagaacaa
     6001 gcagaacgcg ctgccgcgca aagtgaaccg gaaatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca ggggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttagcgc gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgtggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcgtcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctgccgg tcgatattga tgagttagta
     6661 caacgctgcc cgcagctggt acagcagtct gacactgtgg agtgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctgcaaatc ggtctgctga cggtgaaagt gcagccgctg
     6781 gcgaaaccct cggaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt attggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgact ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaagaga tgtttggcga ggccaccaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctggagt tgctttcacc tgcccaaagt
     7261 ccattacaaa tcacacgaga tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa
     7321 aaagagatga aagggcgtta tcccaaacat gtctggccgg acgacccggc aaatactgca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgaaca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcctgcagca
     7921 gtttatggcc gaatggtcaa tcttgagcca gatatgacca tcagcaagaa cgagatggtg
     7981 aagctgctgg aggcgactca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgca tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggctttttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca acgtctgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgc catttctacg agcatgatgg gatcagcctc
     8341 tactcaattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ctcttactgg
     8461 cgtaaagcga acgaagccta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgcggt
     8821 ggggtgatct ctcctcagcc agcctttatg caactggtgc gtcaggagct gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtgg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 ctggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacccgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcag ttacggatac ctggatcaaa ctgggcgtac cgaaagatca gctgcatccg
     9421 gttccggcga tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcgttc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatactt ggtttgcagg cattgacggc agcacggtga ccatcacctg ggcaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcaa cagcaacctg ctcagcaaga gcagaaagac
    10081 agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca tctaagcgtg
    10141 aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga tattaaccct
    10201 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
    10261 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
    10321 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
    10381 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
    10441 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
    10501 gaaagcgcat gggggcctgc tgcaactatt gcggcgaagc actctgctac tgcgactaaa
    10561 acggatacac caattgaaaa aacgccgcag tctatttcgg ttgtgacaaa tgaagagatg
    10621 cagatgcatc aatttcagtc tgtaaaagaa gcattaggtt atacacctgg cgttacggtt
    10681 agcagtcgtg gtgcttctaa tacatatgat tttgttatca ttcgtggttt ctcatctgtt
    10741 ggtctgaatc aaaataatta ccttgatgga ctaaaacttc agggcaactt ttataacgat
    10801 gctgtgattg atccttacat gctcgagagg gttgaactga tgcgtgggcc gacgtctgtt
    10861 ctctatggaa aaagtaatcc tggtgggatt atttctatgg tgagtaagcg tccgaccact
    10921 gaacccctga aagaaattca gtttaaaatg gggacggata atctgtttca gaccggattc
    10981 gatttcagtg atgcactgga tgataacggt gagttctctt accgtttgac gggcctcgct
    11041 cgctcaacaa acgaacagca gaaaaactct gaatctcagc gttataccat tgctccatca
    11101 ttctcatggc gtccagacga caaaaccaat tttactttcc tgtcctattt ccagaatgaa
    11161 ccagaaacgg gttattacgg ttggttgccg aaagagggga ccgttgagcc attgcctaat
    11221 ggtaagcgtc taccgactga cttcaatgaa ggtgcatcga ataatacata ctcccgtaac
    11281 cagaaaatgg tgggatatag ttttgaacat ggttttaatg acaccttcac cgtgcgtcag
    11341 aatctgcgtt tcagtgaaat gaaaacctca cagaaaagtg tttatggcac agggattgct
    11401 ggtgatggtc ataccctaaa ccgcgggaca gtggtggata atgagcgtct acaaaacttt
    11461 agcgttgata cccaacttga aagtaagttt gctacaggtg acgtcgagca tactttgctg
    11521 acaggggtag acttcatgcg gatgcgtaat gatatcaatg ccagctttgg atccgcacca
    11581 tccatcgatc tttataacaa atatcaccct gaatactttg catttggtag cgcagagcca
    11641 taccagatga atgaaagcaa acaaacaggt atttatgttc aggatcaggc ggaatggaat
    11701 aaatgggtat tcactctggg gggacgctac gattggtcta agcaagcgac cactgttcgt
    11761 gaaaactctt atacgccgac tgaaggttat attgagcgca atgatcatca gttcacctgg
    11821 cgcggtggtg taaattactt attcgataat ggtatttcac cttactttag ctatagccag
    11881 tcctttgaac cgagtgcttt cgatctgtgg agcaacccac gcatttccta taagccatcg
    11941 aaaggtgaac agtatgaagc tggcgtaaaa tatgttccga atgatatgcc ggtcgttgtt
    12001 acgggcgcag tctatcaatt gacgaaaaca aataacctga cagcagaccc aacaaaccca
    12061 ttagcgcaag tcccagcagg tgagattcgc gctcgtggtg tggaacttga agcaaaagcg
    12121 gcgttaaatg ccaatattaa cttgacggct tcttatacct acaccgatgc ggaatacacc
    12181 aaagacacca atctcaaagg taaaactcca gaacaagtac cggagcatat ggcatctctc
    12241 tggggggatt ataccttcaa tgaagggccg ctttctggtt taacattggg aacaggtggt
    12301 cgttttattg gttccagcta tggtgatccg gcaaacactt ttaaagtggg tagcgcagct
    12361 gtaatggatg ctgttgtaaa atatgatctg gcacgctttg gtatggcggg atccagcctt
    12421 gctgtgaacg tcaacaattt gctcgatcgt gagtatgttg ccagttgctt ccagacttat
    12481 ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt
    12541 ctcttttggg gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca
    12601 cgaatcattc cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca
    12661 cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc
    12721 acaacggttc tagtaaatcc actctgctca aaatgcttgg ccgtcatcag cctccgtcag
    12781 aaggggagat tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc
    12841 gcaaagtggc ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac
    12901 tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg gcgctttggg gcggcagatc
    12961 gcgaaaaagt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
    13021 ttgatagtct ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
    13081 atagccgttg tctgttgctc gacgaaccga cctcggcgct ggatatcgcc caccaggttg
    13141 atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg cctgacggtc attgccgtgt
    13201 tgcacgatat caatatggcg gcacgctact gtgattatct ggtcgccctg cgcggtggtg
    13261 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgataccctc gaaatgattt
    13321 atggcatccc gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt
    13381 attgatgagc ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc
    13441 tccgttgtta tggcagatga ataccgccca cgcggcggct attgatccca atcgtattgt
    13501 ggcgctggag tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt
    13561 agcggatacc atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat
    13621 cgacgtcggt ttgcgcacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt
    13681 tatggtctgg tcggcaggat atggcccttc agcagaaatg ctggcacgta ttgcgccggg
    13741 gcgtggattt aacttcagcg acggcaaaca gccgctggcg atggcgcgta aatcactgac
    13801 ggaaatggca gatttactta acctgcaaag cgcagcagaa acgcatttag cgcaatatga
    13861 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct
    13921 gacgacgctt atcgatccgc gccatatgtt ggtcttcggt ccaaacagct tgttccagga
    13981 aattcttgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
    14041 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
    14101 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
    14161 gtttgtccgc gccggacgct ttcagcgcgt acccgcagtc tggttttatg gtgcgacgct
    14221 ctcggcaatg cactttgtgc gcgttctgga taacgccatc ggaggtaaag cgtgagtaaa
    14281 cgaattgcgc ttttcccggc gttattgctg gcgctgctgg tgattgtcgc tacggcgctg
    14341 acctggatga acttttcgca ggcgctgccg cgtagccagt gggcgcaggc cgcctggtcg
    14401 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgctgcc gcgtctggcg
    14461 atttcgctgc tggtgggcgc aggcctgggg ctggtgggcg tgctgtttca gcaagtgctg
    14521 cgtaatccgc tggcggaacc gacgacactt ggcgttgcta caggcgcgca actggggatt
    14581 accgtcacta cgctctgggc gatcccaggg gcgatggcga gtcagtttgc tgcgctggcg
    14641 ggcgcttgcg ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg
    14701 gtaacgctga tcctcgcggg gctggtagtg agcctttatt gtggggcaat caatcagtta
    14761 ctggttatct tccaccatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg
    14821 acgcaaaccg actggggcgg tgttgagcgt ttatggccgc agttgctggg cggtgtgatg
    14881 ctgacgttgc tgctacttcg cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc
    14941 aatctcgggc tggccttgtc gctcgcgcgt ctggcggcgc tgtcgctggc gattgtcatc
    15001 agtgcgctgc tggtgaacgc ggtggggatt atcggcttta tcgggttgtt tgcgccgttg
    15061 ctggcaaaaa tgctgggggc gcggcgtctg ctgccgcgac tgatgctggc gtcgttgatt
    15121 ggtgcgctga tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg
    15181 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgttg
    15241 ccgcgtttac gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcggctgaa
    15301 cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg
    15361 gtggcgctgt cgtttggtcg tgatgcgcac ggctggatgt gggcgagcgg ggcgttgctc
    15421 gatgatttaa tgccctggcg ctggccgcga attatggcgg cgctatttgc gggcgtcatg
    15481 ctggcggtgg cgggctgtat tattcagcga ctgaccggca acccgatggc aagcccggaa
    15541 gtgctgggga ttagctccgg cgcagcgttt ggcgtggtac tgatgctgtt tctggtaccg
    15601 ggtaatgctt ttggctggct gttacccgcg ggaagtatcg gtgccgcggt gacgctgttg
    15661 attattatga tcgccgccgg gcgcggtgga ttttccccac accgcatgtt actggcgggg
    15721 atggcgttaa gtaccgcatt caccatgctt ttaatgatgt tgcaggcgag tggtgacccg
    15781 cgaatggcgc aagtgctcac ctggatctct ggttcgacct acaacgccac cgatgcgcag
    15841 gtctggcgta ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtaccgccgc
    15901 tggctgacca ttttacctct gggcggtgat accgcacgag ctgtgggaat ggcgctgacg
    15961 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgacctc gacgatgact
    16021 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggtttt
    16081 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtattc
    16141 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
    16201 acctttatcg gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaattgagt
    16261 gcctcgcccg gtgggcgagg caaaattatc acaacttcgc aaatacccga cgtgcagcat
    16321 cgatggtgtt attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg
    16381 ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt
    16441 ccacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa
    16501 taccgaacat gccgccaacg tggttaacga ccagcggagt tccggcttct tctgccgctt
    16561 ccagcagacc ttctgccaga cgtgttgtca gctcatccag cgtttcgtga acgcccggct
    16621 gcgcgacttc attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg
    16681 tacccgcctg atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac
    16741 cgaatgcgcc taccggcatt ccaccgccga tgattttgcc gaggcaggtt aaatctggca
    16801 ctacgccgta ataatcctgt gcgccagcca gtgccacgcg gaagccggtc atcacttcat
    16861 cgatgatcag caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg
    16921 gctgcggcgg aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct
    16981 cttgcgggta ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta
    17041 aggtatgttt ggcgaaatct gccggaacac ccggcgagtt tggctggcct aacgtgagtg
    17101 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
    17161 taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atggtcgctt
    17221 cagtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
    17281 gcgccatttt cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt
    17341 caatcgcggc attgcggata gccggatggt tatggcccag caccatcggc ccccaggaac
    17401 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
    17461 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
    17521 cagggagcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
    17581 gattcgtaga aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg
    17641 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
    17701 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
    17761 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
    17821 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
    17881 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggca
    17941 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatgga ggcgctggta
    18001 catactgctg ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg
    18061 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc
    18121 ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg
    18181 gtgaaattct ttggcgggct ggggacactc ggcggaggca tggtgctggg gcgcgaaggg
    18241 ccaaccgtgc agatcggcgg caatatcggt cgcatggtac tcgatatttt ccgcctgaaa
    18301 ggtgatgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc
    18361 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
    18421 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
    18481 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
    18541 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
    18601 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc
    18661 aaatgggtgc taatgggcgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca
    18721 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcagg gaatttcagc
    18781 atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta ccaccttact gtgcttctct
    18841 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
    18901 gctttcggaa tggttgtcgt tgagctgttt ccgcaatatc accttgaggc ggggactttt
    18961 gctattgccg gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc
    19021 attttggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt
    19081 cttggcgcaa cactattagc gcaatttacc ggcgggaaac cgctatattc ggagattctt
    19141 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
    19201 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
    19261 aatttgccca attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
    19321 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
    19381 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
    19441 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
    19501 ctggtggttg atccgatgag cctgcaatat ctggtcggtg gttccgttga ctataccgaa
    19561 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
    19621 ggttcttcct ttagcatcta atctgttgtt catatgccgg ataaactatc cggcacatct
    19681 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
    19741 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
    19801 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
    19861 cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc cagtacatca cgaatgatcc
    19921 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
    19981 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
    20041 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
    20101 ttaccattgc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
    20161 gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac cacgcccagt accagaacac
    20221 caaaagggtc catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg
    20281 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctaaaaag
    20341 gtcaggcgct aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata
    20401 cgtgggcttg cacgtcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20461 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct
    20521 gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccaaggaacc cggctgtctt
    20581 taaagatgtt ttctccgcca caaacttcga gtacctggtt ttgaatcgac tcttttccac
    20641 tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20701 attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt tcggccttat
    20761 ccggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20821 tcgcatcgac ccacatcact tttattcccg gcgaagccag ctggtcaacc tgccgctcgg
    20881 cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg cgttccagat
    20941 tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga tagtcggaat
    21001 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    21061 aaagcgtgat gacgcgcggt gccgcgttga gccacagtgg cgcgagaaaa gacagggcga
    21121 ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    21181 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21241 tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt gtggcagaca
    21301 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21361 ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca
    21421 gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21481 ttaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc cgtgacatcc
    21541 gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc ccactttcaa cgttggtgcc
    21601 aggccaccgg cagaaccggt gttaataatc acatctggct tgccgtgttc caacagcaaa
    21661 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21721 ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg acgattttcg
    21781 attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21841 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21901 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21961 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc ggatcttcga
    22021 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca
    22081 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    22141 ccagcaggtg gggcgctaca tcgccaaaga aattctaagc cgtatgaaag agcttaaatt
    22201 actggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg ttgagatgtc
    22261 atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cggcgataaa
    22321 tgattggttt cgccagcgtt tgcacccgga agatgccgaa agccagcctc tgactgacga
    22381 tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaaccgctta acgagctgcg
    22441 gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca ttcgtctggt
    22501 gcatacattg atgaggatga atctcacctg ggcacaggtt ggcggtattt taaaatatac
    22561 ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22621 gggttattat ctttctgaag aagcctatat tgcccggttg cgtaaagaac ttaatttggc
    22681 gctttacagt cgttttccat taacgtggat tatggaagca gccgacgaca tctcctattg
    22741 tgtggcagac cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca
    22801 tcatttgcac gaagcgtggg gccagcatga gaaaggttcg ctcttttcgc tggtggttga
    22861 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22921 tatgtattta cgggtaaaca ccctaaataa actggtaccc tatgcggcac aacgatttat
    22981 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    23041 cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca
    23101 tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    23161 ttatcgtcct ttattaagcc tgtcgttatc cgactttact gaactggtag aaaaagaacg
    23221 ggtgaaacgt ttccctattg aatcgcgctt attccacaaa ctctcgacgc gccatcggct
    23281 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23341 atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23401 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga
    23461 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat
    23521 ctgaagaaca cagaaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac
    23581 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23641 aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat
    23701 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23761 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23821 ggaaggttct ccgttccaga gctctccgtt ctgccagggt ggccagggcg gtaatggtgg
    23881 cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg
    23941 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccagctgag
    24001 cgatggccgt aagttcgacg cgaagatggt cggcaaagat ccgcgctctg atatcgcgct
    24061 gatccaaatc cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact
    24121 gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac
    24181 ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt
    24241 catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa
    24301 cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat
    24361 cggttttgca atcccgagca acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24421 ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactccg aactggcgaa
    24481 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc
    24541 cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat
    24601 cagcagcttt gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac
    24661 cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag
    24721 ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa
    24781 caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca
    24841 gatcggcctg aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat
    24901 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24961 cggcgacagc accatctacc tgttaatgca gtaatctcac tcaacccctt cctgaaaacg
    25021 ggaaggggtt ctccttacaa tctgtgaact tcaccacaac tccatacatc ttcatcatcc
    25081 tttaggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg
    25141 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta
    25201 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgtgggcga attatcggca
    25261 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg
    25321 gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg cggcaaggga
    25381 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25441 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25501 agtcgcggtt gatgcacttg ttggcgcagg atagccgttt gcgggaggaa ctggtgatga
    25561 acctgattca ggcagaggag aatactcccg cacttactga atgggcgcaa cggctgggga
    25621 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggtg
    25681 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
    25741 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25801 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    25861 tgaaagagta cggccggctg cgttttcgcg tttcactggg caactatttt accggtcctg
    25921 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    25981 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    26041 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    26101 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgtcacaat gtgcaaccgc
    26161 tggcaacgtc aaaggcgttg tttatttatc gtaataccct ggagtatcgg cttaatcgta
    26221 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    26281 cgttacaact ggatgaagag cggtaggtta tgcgttaagg ttgaggcggc gctgtctcat
    26341 ttccctcagc ccgaagggga gaggggaatg cccgaattaa ccgtcaattc gggattattt
    26401 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
    26461 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    26521 gcagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
    26581 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
    26641 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    26701 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    26761 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    26821 tagcatttgg cctagcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    26881 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg
    26941 ttctacacca gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa
    27001 ctgcccacgc cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg
    27061 taacgtttat cttcgacagg ggcaaaaggt tctccaacca acggtacgcg cagttgcccg
    27121 ccgtgagtat ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg
    27181 tcgggattat gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg
    27241 cattgtccgg cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg
    27301 atcaccgtag cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt
    27361 aagtgatttt tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc
    27421 gcacattcgg caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca
    27481 aataatacgt aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca
    27541 atcgcgtcag aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa
    27601 agaattttga atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca
    27661 aaccagcctg gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg
    27721 gcagtagcct gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg
    27781 gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg
    27841 ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg
    27901 tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgtga
    27961 ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg
    28021 cccatggaaa tgacggaacc ttcttcgaca atcacccctt caactacttc agagcgtgcg
    28081 ccaatgaagc agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg
    28141 ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg
    28201 gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac
    28261 ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca
    28321 acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt cattggcact
    28381 ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag
    28441 agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct
    28501 acacgcagtg caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg
    28561 gtgtctgcgt tggttggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc
    28621 tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt
    28681 ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagcgca
    28741 cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt
    28801 cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt
    28861 cctggctggt cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa
    28921 aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg acggggctgc
    28981 ggtggctgcc agctactttg cgtcagtact tgctccagac caaaccgaat aacctcatga
    29041 cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata
    29101 ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg
    29161 gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct
    29221 ccacgcgtag cctgcgggct aagcaatacc agtggtttgc ttaaatcatg ctgtaataaa
    29281 tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag
    29341 cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt
    29401 gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt
    29461 cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc
    29521 cacagcgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag atagcgcaga
    29581 cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg
    29641 cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt
    29701 gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga
    29761 atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc
    29821 agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt
    29881 tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg
    29941 taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac
    30001 gcgccgagca cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga
    30061 cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga
    30121 tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg
    30181 ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt
    30241 tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt
    30301 ttttcgtcgg caggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg
    30361 agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg
    30421 ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg
    30481 cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc
    30541 agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca
    30601 tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc
    30661 aagccgccag ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc
    30721 tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat
    30781 ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag
    30841 cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct
    30901 tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc
    30961 tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg
    31021 atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg
    31081 tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac
    31141 tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc
    31201 caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc
    31261 cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt
    31321 tctggaaggg tattcattgt gcgccactca taagattaaa ttatcgcatt aaaaaagccg
    31381 gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt
    31441 gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc
    31501 agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt
    31561 tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt
    31621 ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc
    31681 gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaact tctgaatcgc
    31741 cgcaccgatt tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct
    31801 ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat
    31861 tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc
    31921 gccatctttc agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga
    31981 gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc
    32041 attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac
    32101 atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc
    32161 gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata
    32221 ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt
    32281 ctgaatttcg ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg
    32341 ctgtacacag cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg
    32401 gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa
    32461 caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac
    32521 gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg
    32581 cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat
    32641 gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt
    32701 accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat
    32761 ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg
    32821 cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt
    32881 tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga
    32941 aaagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag
    33001 cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga
    33061 ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt
    33121 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat
    33181 ccgtgctgtg accctgtacc tgggcgctgt agctgcaacc gtacgtgaag gccgttctca
    33241 ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac
    33301 tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac
    33361 atcagtaagt gaccgggatg agcgagcgaa gataacgcat ctgcggcgcg aaatatgaag
    33421 ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc
    33481 ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt
    33541 agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc
    33601 atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa
    33661 aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac
    33721 ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag
    33781 actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca
    33841 gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt
    33901 gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa
    33961 ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa
    34021 ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa
    34081 ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg
    34141 gatatagcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc
    34201 atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc
    34261 aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc
    34321 gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg
    34381 tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat
    34441 cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag
    34501 tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac
    34561 ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct
    34621 tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac
    34681 tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg
    34741 cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga
    34801 acggcctggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg
    34861 ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc
    34921 gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact
    34981 cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag
    35041 ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac
    35101 tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc
    35161 tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc
    35221 gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga
    35281 taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt
    35341 attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt
    35401 gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac
    35461 gattacccgt aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg
    35521 tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag
    35581 atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac
    35641 gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtagaatat tacggcacgc
    35701 cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca
    35761 acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg
    35821 gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag
    35881 aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag
    35941 tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga
    36001 tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa
    36061 tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc
    36121 ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt
    36181 ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc
    36241 tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt
    36301 gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc
    36361 cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa
    36421 cagggtagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt
    36481 gaggatgttg atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt
    36541 gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc
    36601 ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc
    36661 gaacataacg ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct
    36721 gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt
    36781 gagacgccat tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac
    36841 tggtcgatgg ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa
    36901 tacattgaag cgcgttggct atttaacgcc agcgccagcc agatggaagt gctgattcac
    36961 ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg
    37021 ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac
    37081 tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat
    37141 tatgatcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg
    37201 acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc
    37261 cgctttacgg atatcgctgc gttgaattta tcggtactgg aaaaaatgga tatgcgcgaa
    37321 ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa
    37381 gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtctcg ctatttgtta
    37441 gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg
    37501 tcaggtatgc cgtggttttg cacggctttt tttgtatagg cttcagtatt cctgagtacc
    37561 gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga
    37621 aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg
    37681 ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg
    37741 ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag
    37801 tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg
    37861 ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg
    37921 cgataccagt cgctttaact cgcgtttgca agaatgtatt cgtaaatctg aagcgctaac
    37981 agccgggaat accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat
    38041 agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca
    38101 gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt
    38161 agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta
    38221 tgccgaactt tactttatag atgttctctg gcccgatttc gatgaacaag actttgaagg
    38281 ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccggcc cggtgatgaa
    38341 acagcctgat ggggggcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    38401 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    38461 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    38521 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38581 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg
    38641 ggttggtgga ttgtctcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    38701 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38761 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38821 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38881 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38941 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    39001 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    39061 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    39121 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    39181 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    39241 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcact gtgcatgaat
    39301 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    39361 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    39421 tcccgctggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    39481 tccgccacca tgccttcaat aataaatctg ttggccaacg agcggcgatt attgccgcag
    39541 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39601 tgcctggcgt acgtccggtg gttggtgaaa tagcagccaa ttcgatagct gcggaagcac
    39661 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39721 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39781 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39841 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggctg caaattgaac
    39901 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39961 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    40021 tccgggataa cccggggaaa tccttagcgt tagaaatcga aaggcaggga agtctcttgt
    40081 ctttgacatt aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    40141 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    40201 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    40261 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    40321 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    40381 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    40441 cgcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    40501 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40561 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40621 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40681 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40741 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40801 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40861 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40921 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40981 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    41041 cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    41101 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    41161 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    41221 gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct
    41281 tgaaaccctg cgcagctact atctggatcg cggttatgcc cgtttcaaca tcgactctac
    41341 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca ttaccgaagg
    41401 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    41461 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacca aagtgaccaa
    41521 gatggaagat gacatcaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41581 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc
    41641 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41701 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ctgggaagcg atctggtcga
    41761 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41821 acgtgttccg ggtagcccgg accaagtaga tgtagtctac aaggtgaaag aacgtaacac
    41881 cggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41941 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    42001 cgattaccag acctatgctg aattgtcggt aaccaacccg tacttcactg tagatggtgt
    42061 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    42121 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa
    42181 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    42241 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    42301 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    42361 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    42421 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    42481 cgatcacaaa tgggttgttc tggggcgtac ccgctggggt tatggtgatg gtttaggcgg
    42541 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tccgtggctt
    42601 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42661 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42721 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42781 gtttattagc gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac
    42841 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42901 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42961 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    43021 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    43081 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    43141 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagcggca ggtctcggtt
    43201 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    43261 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    43321 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc
    43381 agtccatgaa agcgggcagc gatcgcacta agctggaaaa agacgtgatg gctcagcgcc
    43441 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    43501 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43561 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43621 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43681 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43741 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43801 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43861 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    43921 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43981 cggcgaagct ggggaacaat gtatcgattg gcgctaacgc ggtgattgaa tccggcgttg
    44041 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    44101 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    44161 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    44221 gtggtaactg ggttaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    44281 tcggtgcctg cactaccatc gatcgcggcg cactggatga cactgttatt ggcaatggcg
    44341 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    44401 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    44461 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    44521 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44581 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44641 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44701 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44761 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44821 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44881 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44941 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    45001 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    45061 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    45121 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    45181 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    45241 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    45301 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcggaattgg
    45361 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    45421 taatgagatt tatcagttcg cctccatcgg cgaagttaac caggatctga aatatgctgg
    45481 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45541 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45601 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45661 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45721 ccatcagttc tgcatcattg gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca
    45781 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45841 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45901 taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45961 ggcggaaaca tatccggaag tgaaagcctt taccgatttc ttttcacgct caacgcgcgg
    46021 tctgattcgt taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    46081 tccggcgata tcctaggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    46141 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    46201 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    46261 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    46321 attgatgcgc ctgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    46381 accattcatt acgtcagtcc gtcagtctgg gcgtggcgac agaaacgtgt tttcaaaata
    46441 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    46501 tacaacgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc attagatcca
    46561 gataaaaatg ccgcccgtga tgtgctgggg atccctcacg atgcccactg cctggcgttg
    46621 ctaccgggga gccgtggtgc agaagttgaa atgcttagtg ccgatttcct gaaaacggcc
    46681 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccactggt gaatgccaaa
    46741 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacttttc agttcatttg
    46801 ctggatggga tgggccgtga ggcgatggtc gccagcgatg cggcgctact ggcgtcgggt
    46861 acggcagccc tggagtgtat gctggtgaaa tgcccgatgg tggtgggata tcgcatgaag
    46921 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46981 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    47041 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa tcagccacgc gatgcacgat
    47101 accttccgtg aactgcatca gcagatccgt tgcaatgccg atgagcaggc ggcacaagcc
    47161 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    47221 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    47281 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    47341 tggcgctctg tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    47401 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    47461 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    47521 taccgatgcc tgcgatggtt gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg
    47581 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47641 cgcaatatgg ttttgcccaa cacaaagggt acccaaccgc ttttcatctg gaaaaactgg
    47701 ctgaacacgg cgcgaccgaa caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg
    47761 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47821 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa
    47881 ctgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47941 tcaccaacct ttgtggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    48001 ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct gggtgatgag ttaacccacc
    48061 tgacggtact ggcggcgaac aataccggct atcagaatct gacgttgctg atctcaaaag
    48121 cgtatcagcg cgggtacggt gccgctgggc cgatcatcga tcgcgactgg cttatcgaat
    48181 taaatgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    48241 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    48301 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    48361 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    48421 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    48481 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48541 aggagatgtg tgagctgttt gccgacatcc ccgaagccct tgccaacacc gttgagatcg
    48601 ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48661 gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc
    48721 tggccttttt attccctgac gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac
    48781 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48841 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48901 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48961 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    49021 ttgacttctg tatggagaaa cgcgatcagg ttatcgagca cgtagcggac atgtacggtc
    49081 gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg
    49141 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc
    49201 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    49261 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    49321 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    49381 attttgcgcc gctttactgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    49441 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    49501 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc
    49561 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49621 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggatctgatc aagcgtctgc
    49681 aacctgactg cttcgaagat atgatcgcac tggtggcgct gttccgccca ggtccgttgc
    49741 aatcagggat ggtggataac tttatcgacc gtaaacatgg tcgcgaagaa atctcctatc
    49801 cggacgtaca gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatca
    49861 tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat accctcggtg
    49921 gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49981 gttctgtatt tgctgaaggt gcagaaaaga acggagtcaa cgccgaactg gcgatgaaaa
    50041 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    50101 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    50161 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    50221 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    50281 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    50341 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    50401 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    50461 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    50521 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50581 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50641 ggccggagca ggtggtgtta gatggggaac gtgaaacgtt aggtctgtac cttacgggac
    50701 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50761 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50821 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50881 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50941 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    51001 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctagcgggc
    51061 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    51121 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    51181 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    51241 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    51301 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    51361 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    51421 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacacg taaaatcttc gccgatctcg
    51481 gtgcatggca gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg
    51541 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata
    51601 aagctatcgt cggtggtatc gcccgtcttg acggtcgtcc ggtgatgatc attggtcatc
    51661 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51721 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51781 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51841 aagccattgc acgcaacctg cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg
    51901 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51961 tgctgcaata cagctcctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    52021 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    52081 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    52141 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    52201 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    52261 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    52321 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    52381 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    52441 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagt gttgatttgc tgaaatttat
    52501 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52561 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52621 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52681 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52741 taacattacg ccgccgttca cgaaagcctt gtttacctac gttaaagagc ggaagtacac
    52801 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgcct
    52861 gttttatgat tttttcggcg ggaataccct caaggctgat gtctctattt cggtcaccga
    52921 gcttggttcg ttgctcgacc acaccgggcc acacctggaa gcggaagagt acatcgcgcg
    52981 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    53041 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca attgtcataa
    53101 atcgctggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    53161 taatgcgttg gggattcttg gtgggatccc gcaccgtgaa tttactcgcg acagcatcga
    53221 agagaaagtc gctgccacca cgcaggcaca atggccggtt catgcggtga ttaccaactc
    53281 cacctatgat ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    53341 gatccacttc gattccgcct gggtgccgta cacccatttt catccgatct accaggggaa
    53401 aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc
    53461 tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
    53521 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc
    53581 gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca
    53641 aagaggtcca gcggttgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
    53701 cgcaggtgga tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg cacggcttta
    53761 acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt ttgataccgg
    53821 ggatggacga acagggcaat atgagcgagg aggggatccc ggcggcgctg gtggcaaaat
    53881 tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct
    53941 ttagtattgg tatcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
    54001 aacgctctta cgatctcaac ctgcggatca aaaatatgct acccgatctc tatgctgaag
    54061 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
    54121 ttcgtaaaca cgatcttccc ggtttgatgt tgtgggcatt cgatactttg ccggaaatta
    54181 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
    54241 tggaacaact ggtcggtaga gtatcggcaa atatgagcct gccttatcca ccgggcgtac
    54301 cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc gattttctac
    54361 tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt cacggcgcga
    54421 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
    54481 gagcggctta cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
    54541 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca aagctttcta
    54601 ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
    54661 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc
    54721 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
    54781 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
    54841 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
    54901 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
    54961 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac
    55021 gccagatttt ggtcgccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
    55081 tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt
    55141 taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
    55201 tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg
    55261 cgcggcaggc gcgttatcag gcatttgccc gtaccctatt gcccggcgaa gtgctggtca
    55321 ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg
    55381 gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc
    55441 gcccgttgct tgcccgcacg cggggggaac tggcgcagtg ggcattggcg catggtttac
    55501 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
    55561 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg
    55621 ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac
    55681 actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc
    55741 gccgcgcggc gattatccgt cgctggctgg cagggcagaa tgcaccaatg ccttcccgcg
    55801 aagcgctggt gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt
    55861 tacgcttggg cgcgtttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg
    55921 tcaccgggca aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg ctggaattac
    55981 cggcggggct gggaagcgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag
    56041 acgaagcggt cagcgtgcgt ttcaaatcgg caggattgct gcatattgtc gggcgcaacg
    56101 gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca
    56161 ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc
    56221 aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt
    56281 aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagtcaa tcaggactcg
    56341 ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc cagacgtagc
    56401 tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa
    56461 tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
    56521 tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg atacgtatca
    56581 ttcattaata taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
    56641 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
    56701 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
    56761 ctcacaatat ttttccacat gccctcctgt cagcacactg acttaaccgt ggatgcaagt
    56821 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
    56881 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
    56941 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
    57001 aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt
    57061 atgatgctgc gcctgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt
    57121 ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat
    57181 ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg cacccaggcc
    57241 gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat
    57301 cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg
    57361 gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggc
    57421 gtgatctggt tatcggacga taagaataat ctggaagtta acttaaccgc ctggcaacaa
    57481 ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg
    57541 ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg gccattcatc
    57601 tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt ctgctcgccg
    57661 ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca
    57721 gtcaggaact gaaccgcgaa gccgcgctag cccggctggt ggcagtgatt aaagatttaa
    57781 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
    57841 tggcatcgaa agcacaaaaa tcaagcgcga aggcgatgcg cggcaaagta cgcagcggtc
    57901 gggaataaaa agaaggaata gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
    57961 agcctcttta ctctgatggg atgtaataac cgggccgaag tcgatacgct ttctccagca
    58021 caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgctgat
    58081 tgtgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
    58141 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
    58201 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
    58261 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca gttcaactat
    58321 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
    58381 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
    58441 gcgaataacg cagagctgga gcgtggctac ctggctgcgc gcggtcacag tgaaaaaccg
    58501 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
    58561 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
    58621 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa acaggccaga aggatatatt
    58681 tcaacatttt gaatttgcac gttttttgta ggccggataa ggcgtttacg ccgcatccgg
    58741 caatggtgct caaagcctga tgcgacgctg gcgcgtctta tcatgcctac aacccccctc
    58801 ataccttaag gcggataagg catttacttt atcaccgggt caaaccaaca aagcgacccg
    58861 ccttgatata aatccctggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58921 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58981 gcgccagtgt aagtggatgt ccggctttgg caaatgcatc agtgacaaat tccgaacaaa
    59041 accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga
    59101 cgcactgctg gcgaaaatcc tcggagaact gattcagtga gcacatctgg cgagtcacca
    59161 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    59221 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    59281 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    59341 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    59401 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    59461 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    59521 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg gctggctgat
    59581 atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc agtacgcctt
    59641 tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag gaccctttct
    59701 ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc ttaattaact
    59761 gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg ttacggtcgc
    59821 ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc acgcccggac
    59881 gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg ctgtacagtt
    59941 tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga atcgccacct
    60001 ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc tgctcaatcg
    60061 ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg atttggttac
    60121 ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta cccagctgga
    60181 agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca tccgggcttg
    60241 gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca tcgcgatccc
    60301 agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa tcactcatcg
    60361 ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc agtgaacccg
    60421 gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa
    60481 cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc accagcagcg
    60541 caaccagcgg gaagctgctg ccttcaaccg ctttaaccag cagagtctta accgttttct
    60601 caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc ggcgtatcaa
    60661 ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata gcttctgcca
    60721 gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct tcaccgctct
    60781 gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg tcggcttgca
    60841 cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca tacattgcat
    60901 cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct ttcatcagga
    60961 attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac ttggtctgga
    61021 tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga atcaggtcag
    61081 tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg tcaacaaaac
    61141 gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa tcggctggct
    61201 gaaccaccgg catcgacacc tcgattgcac cggcgttgtt catctcttca cgcacgatgt
    61261 tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac ccggaggcca
    61321 gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg gcgtcggcag
    61381 gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta cggttccagt
    61441 tggaaggtag aacaggctca aggcgagcct gggacaaaaa agtgatttag tttaccagtg
    61501 caaaagaaat gtcaaaagag aagggcgtga atttaacgcg gttccagcgc aaagacttca
    61561 aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat gtagccagac ggcataggtt
    61621 ttgcccgttt cctcaccttt atgataggcc gggcgcgggt cctgcgccag tacttcgcgg
    61681 ataaacagcg ttaactgcgg ataacgcttc tccagcgtca aaagctgctt ttcgacctct
    61741 gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt gcgcatagct ggcactggca
    61801 tcgggaagcg attcggcaaa ggggagatac ggtttgatat ccactaccgg cgtaccatcg
    61861 accagatcca gactgccgag cttcagaatc acgctgtctt tatggcaaac aacctctttc
    61921 agctctacca gcgacatacc aattgggtta gggcggaaag tagagcgtgt tgcgaaaacc
    61981 cccattctgg cgttaccgcc gaggcgcggt ggacgcacag tcggacgcca gccgccttcc
    62041 atcgtttgat gaaagacgaa aaggatccat aaatggctga acgcttccag gccgcgaacg
    62101 gcgtcggcct ggttgtaggg agcaatgaga tgcagttcgc cgttggcgct ttttaccaga
    62161 cctggctggc gcggaacggc gaatttttct ttatagggcg agcgaataac gcctatttgc
    62221 tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc agagccgata catacagcct
    62281 gacgatagca gcctggcgta ccgctggtga cttcgcagct atgcagtaat accgcattgg
    62341 ctttcatttt agaggcgttg atttgcatcc gcttacgcgc ggttggaatg ctcggcggag
    62401 agtcctgatt agaggcctgg caagagtcgc cactgacttc accgagatcg cggaacggtt
    62461 tgccgactaa ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc ggcgctttcg
    62521 gttttgcagg ctccgctttc ggctggggtg cagtgctttg aacgggttcg acaggggatc
    62581 tgcttaacat ggaacagccg cttagcatga gtgctactaa acagatcggt aaagcacgca
    62641 tagtatttcc tcaatgtatg atcaaaacgt caatattgaa tcaggagctt gtaaaaatga
    62701 caagacgggc aagtgcccat cctgaatgat attacaaatt gtggaaacag cctaaaaatt
    62761 accagccttt aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa acttcatcag
    62821 actgataagc ctggacgaat ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta
    62881 cgatcaggtt tacgtacggg gagtctttat cttcgacaaa gataccgtct ttcgctggag
    62941 tcaggccaat ctggctggca taggtggtat tgataactgc cagagcgatt tgtgcgtcgt
    63001 ccagagatcg aggcagctgc ggtgcttcca gctcaacaat tttcagattt tttgggttct
    63061 caacaacatc aagtacggtc ggcagcaggc caacgccatc tttcagtttg atcaagccca
    63121 ctttttgcag cagcagcagt gaacgaccaa ggttagttgg atcgtttggc acggcaacct
    63181 gcgaaccatc ctgcagttca tccagtgatt tgattttctt ggagtaacca gcaatcggat
    63241 aaacaaaagt gttgcctact gcgaccagtt tgtagccacg atctttcagt tgctgatcaa
    63301 ggtacggttt atgctggaag gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg
    63361 gcagaacata gtcgttgaag gttaccagct caacgtccag gccatatttg tctttcgcaa
    63421 ctttctgcgc gacttctgca acctgctgtt cggcaccaac aatcacgccg actttaatgt
    63481 ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca atcagggctc
    63541 ccactgccgc aaaggttttg aatttgaacg ccatacctta ttccttcttc aattatttat
    63601 gttgtgttga acgttacttg cgagtgacag cccggacgat gcggtcgcct gcgaactgaa
    63661 ttaaataaac cagaatgacc agcaatacca gtaccgtatt catcaccgtc gcgttatagc
    63721 cgatgtagcc atactgatag ccaatctgac ctaaaccacc ggcaccgaca gcaccaccca
    63781 tcgcggaata accgactagg gtaatcaggg tgatagttgc cgcattcacc agacccggca
    63841 atgcttccgg taacagcact ttacggacga tctgcatcgg cgtggcaccc attgcacggg
    63901 aagcttcaat taacccggtt gggatctcca gcagagcgtt ctcgaccata cgggcaataa
    63961 acggtgctgc accaacggtt aacggaacaa tcgctgcctg caaaccaatc gatgtaccaa
    64021 caataacgcg ggtaaacgga atcatccata ccagcaagat aatgaacggg atagaacgga
    64081 aaatgttcac aatcgcagaa acggtacgat acagcttcgc gttagcaata atttgccccg
    64141 gacgcgtgac ataaagcaga acgccaaccg gcagaccaat cacaaagcca aaaaaaccgg
    64201 atacgaaggt cattgccagc gtttcccata cgccacgaac cagcagccac atcatcggct
    64261 cagacataac ccagtacctc tacttttaca tggtgttcct gcagccaggc aatggcggct
    64321 tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga tgccgaactt aacgccaccg
    64381 gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg cgcggtttca
    64441 gaaagcagtg gggcatcgac cgattgaccg gtaaactcca gacgcagcat cggcacgcag
    64501 tcagtaaatg gctccgcttg cagacgttcc tggtaatctt ccgggatatc cagatgcagg
    64561 gtcgactgaa taaacttctg cgccagcggc gttttcggat gcgagaacac ttcacttacc
    64621 gtgtcctgct cgatcagttc gccattgctg atgaccgcca cgcaatcaca aatacgcttc
    64681 acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg gttaatgtct
    64741 ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca gcgcgctggt ggcttcatca
    64801 cacagcaata ctttgggatt gctggctaat gcacgggcaa tcgccacacg ttgtttctgc
    64861 ccaccggaaa gattcgacgg gtagctatca tgcttatcgc caagaccaac caatgacagc
    64921 aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt tgtccagctc cagcggcaga
    64981 gccacgttgc caaaaacagt acgcgaagag agcaggttaa aatgctggaa aatcatacca
    65041 atctggcggc gagctttggt caactcggat tctgacagcg tggtcagttc ctggccatcg
    65101 accagcacgc taccctcggt tgggcgctcc agcaggttta cacaacgtat aagcgtactc
    65161 ttacccgcgc ctgaggcacc gataacgcca taaatttgtc cagctggcac atgcaggctg
    65221 acgttgttca acgcctggat gatgcgggtg ccctggtgga acactttggt gatattcgaa
    65281 agttttatca ttgattattt attatcgtca ttaagttagt cgtggcatct cgaatgcctg
    65341 aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc cagacgtcta aatcaatcag
    65401 gtttatgcga agagcacttt cttgcaggtc gaaacatgcg atactagcgt cacatgcctt
    65461 attaaggagc tataaaaggt ggcaaagagc gtacccgcaa tttttcttga ccgtgatggc
    65521 accattaatg tcgatcacgg ctatgtccat gagatcgaca actttgaatt tatcgacggt
    65581 gttattgacg ccatgcgcga gctaaaaaaa atgggctttg tgctggtggt agtaaccaac
    65641 cagtctggca ttgctcgcgg taaatttacc gaagcacagt ttgaaacgct gaccgagtgg
    65701 atggactggt cgctggcgga ccgagatgtc gatctggatg gtatctatta ttgcccgcat
    65761 catccgcagg gtagtgttga agagtttcgc caggtctgcg actgccgtaa accacatccg
    65821 gggatgcttt tgtcagcacg cgattatttg catattgata tggccgcttc ttatatggtg
    65881 ggcgataaat tagaagatat gcaggcagcg gctgcggcga acgtgggaac aaaagtgctg
    65941 gtgcgtacgg gtaaacctat tacgcctgaa gcagaaaacg cggcggattg ggtgttaaat
    66001 agcctggcag acctgccgca agcgataaaa aagcagcaaa aaccggcatg atgattaaaa
    66061 gatgagcgat tgaaataaaa atgcattttt ccacttgtct tcctgagacg actccctata
    66121 atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga
    66181 agagaaaaat cctgaaattc agggttgact ctgaaagagg aaagcgtaat atacgccacc
    66241 tcgcgacagt gcgctaaagt gcgtcgcaac tgctctttaa caatttatca ggcaatctgt
    66301 gtgggcactc gaagatacgg attcttaacg tcgcaaggcg aaaaatg
//
LOCUS       DAJVTY010000005        64031 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVTY010000005 DAJVTY010000000
VERSION     DAJVTY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64031
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.005"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..188)
                     /locus_tag="OQQ66_000315"
     CDS             complement(<1..188)
                     /locus_tag="OQQ66_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1903365.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWAL"
     gene            complement(190..576)
                     /locus_tag="OQQ66_000316"
     CDS             complement(190..576)
                     /locus_tag="OQQ66_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1903366.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            607..1173
                     /locus_tag="OQQ66_000317"
     CDS             607..1173
                     /locus_tag="OQQ66_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1903367.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1316..2488
                     /locus_tag="OQQ66_000318"
     CDS             1316..2488
                     /locus_tag="OQQ66_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCS1903368.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2498..3013
                     /locus_tag="OQQ66_000319"
     CDS             2498..3013
                     /locus_tag="OQQ66_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCS1903369.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3068..3370
                     /locus_tag="OQQ66_000320"
     CDS             3068..3370
                     /locus_tag="OQQ66_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1903370.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3385..3504
                     /locus_tag="OQQ66_000321"
     CDS             3385..3504
                     /locus_tag="OQQ66_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCS1903371.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3497..6574
                     /locus_tag="OQQ66_000322"
     CDS             3497..6574
                     /locus_tag="OQQ66_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1903372.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMVD
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6571..7056
                     /locus_tag="OQQ66_000323"
     CDS             6571..7056
                     /locus_tag="OQQ66_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1903373.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            7053..8153
                     /locus_tag="OQQ66_000324"
     CDS             7053..8153
                     /locus_tag="OQQ66_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCS1903374.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            8247..8465
                     /locus_tag="OQQ66_000325"
     CDS             8247..8465
                     /locus_tag="OQQ66_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1903375.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8701..10386)
                     /locus_tag="OQQ66_000326"
     CDS             complement(8701..10386)
                     /locus_tag="OQQ66_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1903376.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTAHSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10656..11033
                     /locus_tag="OQQ66_000327"
     CDS             10656..11033
                     /locus_tag="OQQ66_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1903377.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11063..11320)
                     /gene="grxA"
                     /locus_tag="OQQ66_000328"
     CDS             complement(11063..11320)
                     /gene="grxA"
                     /locus_tag="OQQ66_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1903378.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11480..11767
                     /locus_tag="OQQ66_000329"
     CDS             11480..11767
                     /locus_tag="OQQ66_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1903379.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            11751..12473
                     /gene="nfsA"
                     /locus_tag="OQQ66_000330"
     CDS             11751..12473
                     /gene="nfsA"
                     /locus_tag="OQQ66_000330"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1903380.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12534..13436
                     /gene="rimK"
                     /locus_tag="OQQ66_000331"
     CDS             12534..13436
                     /gene="rimK"
                     /locus_tag="OQQ66_000331"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1903381.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIVHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            13524..14000
                     /locus_tag="OQQ66_000332"
     CDS             13524..14000
                     /locus_tag="OQQ66_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1903382.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14351..15463
                     /gene="potF"
                     /locus_tag="OQQ66_000333"
     CDS             14351..15463
                     /gene="potF"
                     /locus_tag="OQQ66_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1903383.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15558..16691
                     /gene="potG"
                     /locus_tag="OQQ66_000334"
     CDS             15558..16691
                     /gene="potG"
                     /locus_tag="OQQ66_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1903384.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTQLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16701..17654
                     /gene="potH"
                     /locus_tag="OQQ66_000335"
     CDS             16701..17654
                     /gene="potH"
                     /locus_tag="OQQ66_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1903385.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17651..18496
                     /gene="potI"
                     /locus_tag="OQQ66_000336"
     CDS             17651..18496
                     /gene="potI"
                     /locus_tag="OQQ66_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1903386.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18556..19044
                     /locus_tag="OQQ66_000337"
     CDS             18556..19044
                     /locus_tag="OQQ66_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1903387.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19085..20212
                     /gene="rlmC"
                     /locus_tag="OQQ66_000338"
     CDS             19085..20212
                     /gene="rlmC"
                     /locus_tag="OQQ66_000338"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1903388.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(20387..21118)
                     /gene="artJ"
                     /locus_tag="OQQ66_000339"
     CDS             complement(20387..21118)
                     /gene="artJ"
                     /locus_tag="OQQ66_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1903389.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(21409..22077)
                     /gene="artM"
                     /locus_tag="OQQ66_000340"
     CDS             complement(21409..22077)
                     /gene="artM"
                     /locus_tag="OQQ66_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1903390.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(22077..22793)
                     /gene="artQ"
                     /locus_tag="OQQ66_000341"
     CDS             complement(22077..22793)
                     /gene="artQ"
                     /locus_tag="OQQ66_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1903391.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(22800..23531)
                     /gene="artJ"
                     /locus_tag="OQQ66_000342"
     CDS             complement(22800..23531)
                     /gene="artJ"
                     /locus_tag="OQQ66_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1903392.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(23549..24277)
                     /gene="artP"
                     /locus_tag="OQQ66_000343"
     CDS             complement(23549..24277)
                     /gene="artP"
                     /locus_tag="OQQ66_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1903393.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(24495..25010)
                     /locus_tag="OQQ66_000344"
     CDS             complement(24495..25010)
                     /locus_tag="OQQ66_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1903394.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            25136..25459
                     /locus_tag="OQQ66_000345"
     CDS             25136..25459
                     /locus_tag="OQQ66_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1903395.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25456..26286
                     /gene="amiD"
                     /locus_tag="OQQ66_000346"
     CDS             25456..26286
                     /gene="amiD"
                     /locus_tag="OQQ66_000346"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1903396.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26283..27293)
                     /locus_tag="OQQ66_000347"
     CDS             complement(26283..27293)
                     /locus_tag="OQQ66_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1903397.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(27392..28822)
                     /locus_tag="OQQ66_000348"
     CDS             complement(27392..28822)
                     /locus_tag="OQQ66_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1903398.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28833..29834)
                     /gene="ltaE"
                     /locus_tag="OQQ66_000349"
     CDS             complement(28833..29834)
                     /gene="ltaE"
                     /locus_tag="OQQ66_000349"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1903399.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29871..31589)
                     /gene="poxB"
                     /locus_tag="OQQ66_000350"
     CDS             complement(29871..31589)
                     /gene="poxB"
                     /locus_tag="OQQ66_000350"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1903400.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31722..32690)
                     /gene="hcr"
                     /locus_tag="OQQ66_000351"
     CDS             complement(31722..32690)
                     /gene="hcr"
                     /locus_tag="OQQ66_000351"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1903401.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32702..34354)
                     /gene="hcp"
                     /locus_tag="OQQ66_000352"
                     /gene_synonym="priS"
     CDS             complement(32702..34354)
                     /gene="hcp"
                     /locus_tag="OQQ66_000352"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1903402.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34498..35397)
                     /gene="lysO"
                     /locus_tag="OQQ66_000353"
     CDS             complement(34498..35397)
                     /gene="lysO"
                     /locus_tag="OQQ66_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1903403.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35891..36586)
                     /gene="aqpZ"
                     /locus_tag="OQQ66_000354"
     CDS             complement(35891..36586)
                     /gene="aqpZ"
                     /locus_tag="OQQ66_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1903404.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37012..38670
                     /locus_tag="OQQ66_000355"
     CDS             37012..38670
                     /locus_tag="OQQ66_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1903405.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38667..39623)
                     /locus_tag="OQQ66_000356"
     CDS             complement(38667..39623)
                     /locus_tag="OQQ66_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCS1903406.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39774..40889
                     /gene="macA"
                     /locus_tag="OQQ66_000357"
     CDS             39774..40889
                     /gene="macA"
                     /locus_tag="OQQ66_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1903407.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            40886..42832
                     /gene="macB"
                     /locus_tag="OQQ66_000358"
     CDS             40886..42832
                     /gene="macB"
                     /locus_tag="OQQ66_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1903408.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(42905..43129)
                     /gene="cspD"
                     /locus_tag="OQQ66_000359"
     CDS             complement(42905..43129)
                     /gene="cspD"
                     /locus_tag="OQQ66_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1903409.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43452..43772
                     /gene="clpS"
                     /locus_tag="OQQ66_000360"
     CDS             43452..43772
                     /gene="clpS"
                     /locus_tag="OQQ66_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1903410.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            43803..46079
                     /gene="clpA"
                     /locus_tag="OQQ66_000361"
     CDS             43803..46079
                     /gene="clpA"
                     /locus_tag="OQQ66_000361"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1903411.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46423..46510)
                     /locus_tag="OQQ66_000362"
     tRNA            complement(46423..46510)
                     /locus_tag="OQQ66_000362"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46474..46476),aa:Ser,seq:gga)
     gene            complement(46764..46982)
                     /gene="infA"
                     /locus_tag="OQQ66_000363"
     CDS             complement(46764..46982)
                     /gene="infA"
                     /locus_tag="OQQ66_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1903412.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47267..47971)
                     /gene="aat"
                     /locus_tag="OQQ66_000364"
     CDS             complement(47267..47971)
                     /gene="aat"
                     /locus_tag="OQQ66_000364"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1903413.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48013..49734)
                     /gene="cydC"
                     /locus_tag="OQQ66_000365"
     CDS             complement(48013..49734)
                     /gene="cydC"
                     /locus_tag="OQQ66_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1903414.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(49735..51501)
                     /gene="cydD"
                     /locus_tag="OQQ66_000366"
     CDS             complement(49735..51501)
                     /gene="cydD"
                     /locus_tag="OQQ66_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1903415.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51624..52589)
                     /gene="trxB"
                     /locus_tag="OQQ66_000367"
     CDS             complement(51624..52589)
                     /gene="trxB"
                     /locus_tag="OQQ66_000367"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1903416.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53134..53628
                     /gene="lrp"
                     /locus_tag="OQQ66_000368"
     CDS             53134..53628
                     /gene="lrp"
                     /locus_tag="OQQ66_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1903417.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            53763..57791
                     /locus_tag="OQQ66_000369"
     CDS             53763..57791
                     /locus_tag="OQQ66_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1903418.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            57946..58557
                     /gene="lolA"
                     /locus_tag="OQQ66_000370"
     CDS             57946..58557
                     /gene="lolA"
                     /locus_tag="OQQ66_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1903419.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58568..59911
                     /gene="rarA"
                     /locus_tag="OQQ66_000371"
                     /gene_synonym="mgsA"
     CDS             58568..59911
                     /gene="rarA"
                     /locus_tag="OQQ66_000371"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1903420.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60002..61294
                     /gene="serS"
                     /locus_tag="OQQ66_000372"
     CDS             60002..61294
                     /gene="serS"
                     /locus_tag="OQQ66_000372"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1903421.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61533..63977
                     /gene="dmsA"
                     /locus_tag="OQQ66_000373"
                     /pseudo
     CDS             61533..63977
                     /gene="dmsA"
                     /locus_tag="OQQ66_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
BASE COUNT        15747 a        16080 c        17259 g        14945 t
ORIGIN      
        1 agtgcccatg tgggtaatcc attttttcct gcaacacgaa ttttgtcatc tgggggcgta
       61 ttgattgcaa attcattata aacatcatca ctgacagcca gagcatcatc tggccatgaa
      121 tttgcattaa tgtaatcatc cttaagcgca ggattcacaa aaatgtttaa agatggacta
      181 taaaacatat taaaccccta tagcgatata acgacctaat acagcatttg cagcagtcgc
      241 tatgcttgaa aaaccgcgga actgatttga tgtaattgca gaagccgaca ggattcctgc
      301 gcctgaaggt gtatgcccca catgactaac tatcatccca taacatgctg acggaaaggc
      361 aaaaggaaaa tcattaagat atcctgcatc ttcaccacca gacccaccaa atctcgcctg
      421 cccccactga ataatcagtg ttctccggga acctgaaata attaacggaa tcgttacata
      481 cccattcagg ccaatgacac ccgatgcagt gccagccaga gataattctc ccaaaccaac
      541 gtttaagaaa atgcagagat aatggctaac tggcatcatc cccggttttt attcagggga
      601 ttgatcatgc ttattggcta tgtacgcgtg tcaacaaatg accagaacac cgatttgcaa
      661 cgtaatgcac tgaactgcgc gggatgtgag cggatttttg aggataaaat cagtggcact
      721 aagcccgaca gaccggggct taaaaaactg ctcaggacac tatcggcagg agacacgctg
      781 gttgtctgga agctggacag actgggacgc agtatgcggc atcttgttac gctgatagaa
      841 gagttgcgcc agcgtggtgt gaatttccga agcctgactg acagtattga taccagtacc
      901 ccaatgggcc gtttcttttt tcacgtcatg ggggctctgg ctgaaatgga acgtgaactg
      961 atagttgaac gtaccagagc agggctggct gcagctcgtg ccaaaggcag agtaggtgga
     1021 cgccgtccta agttgaccac cgaacagtgg gcacagattg gacgtttact cgaggccgga
     1081 gaatcaagac agcgtattgc actgattttt gatgtgggcg tttctaccat ttatagaaaa
     1141 tttccggcga atgagagcaa tgaatcgccc tgagtcagta ttattttgat ttttcctgta
     1201 agcggacaaa taccgtcatt ttgtgtgaat aacgacacaa ctgcgcttag ctgtttgtca
     1261 ggcacaatca cttcaacata gggcgaagcc taatccaatc aggaggttcg ccactatggc
     1321 tcaggattac caccacgggg tgcgcgttgt tgaagtcaac gaaggcaccc gatccattac
     1381 cacggtgagc accgccatcg tgggtatggt ctgcacgggc gatgatgccg atgcaaaaat
     1441 gtttcctctt aataaacctg tgctgatcac tgatgtgctg accgccagcg gtaaagcggg
     1501 tgagtctggc acgctggccc gttcgctgga tgccatcgct gaccaggcaa aacccgtgac
     1561 cgttgttgtg cgtgtgccgc agggtgaaac ggaagaagaa accacaacca atatcattgg
     1621 cgcagtgacc gccgagggta aaaaaacagg tatgaaagcc ctgttatctg cccagtcaca
     1681 gctcggcgtt aaaccgcgca ttctcggcgt gccaggtcac gacacgaagg cggtagctac
     1741 tgagttgctg agcgtggcgc aaagcctgcg tggatttgct tacctgtcag cgtatggttg
     1801 caagacggtg caggaagcaa tcacttaccg tgaaaacttc agtcagcgtg aaggaatgct
     1861 gatctggccc gactttactg gctgggacac ggtgctgaat gccgaagcaa cggcttatgc
     1921 caccgcccgt gcgctcggtc tgcgcgctaa aattgatgag cagaccggat ggcacaaaag
     1981 cctgtccaac gtgggcgtga acggtgtcac cggaatttct gctgatgtgt tctgggatct
     2041 gcaggacccg gcaaccgatg caggtctgct taaccagaac gatatcacca cgcttatccg
     2101 caaagacggt ttccgcttct ggggttcccg ctgtctgagc gatgatccgc tctttgcctt
     2161 cgaaaactac acccgcacgg cgcaggtgct gatggacacg atggcagaag cgcacatgtg
     2221 ggcggtggac aaaccgctga acccgtcgct ggcccgcgac attatcgaag gtatccgcgc
     2281 caaaatgcgt agcctggtca gtcagggcta tctcattggt ggtgattgct ggctggacga
     2341 gtcggtgaac gataaagaca cgctgaaagc cgggaaactc accatcgatt acgactacac
     2401 gccagtgccg ccacttgaaa acctgatgct gcgtcagcgc atcaccgatc agtacctggt
     2461 gaatttctcc agccaggtca gcgcgtaagg ggacaacatg gctttaccac gcaaattaaa
     2521 acacctgaac ctgtttaacg acgggaacaa ctggcagggg atcgttgagt cgctgacgct
     2581 gccgaaattc acccgcaaat atgagaagta tcgcggcggc ggtatgcccg gagcggtgga
     2641 tgtggatctg ggactggatg acagtgcact ggatacagaa ttttccattg gtggtactga
     2701 actgctgctg tttaagcaga tgggcaaagc cacggtggat ggcatccagc tgcgctttac
     2761 cggctctatt cagcgtgatg ataccgggga agtgcaggcc gtggagcttg tggtgcgtgg
     2821 acgtcacaaa gaagtagatt ccggcgagtg gaagacgggc gaaagcaaca ctaccaaagt
     2881 gaccagtacc aacagctacg cgaagctgac catcaatggt gaggtgctct atgaagtgga
     2941 cctgatcaac atggtggaaa ttgtggacgg tgtggacctg atggaagcgc accgcaacgc
     3001 cctcggcctc tgatgtatct gaacggcgcg gaatgccgcg ccagaaccta atttacagga
     3061 caacaaaatg agcgataagc agactgaaaa gaccattcaa ctggatactc ccatcatgcg
     3121 cggtaaaaca gaaattaccg aaattgtgtt gcgtaaaccg cagtccggtg cgctgcgcgg
     3181 cacacgcctg caggccatta tggatatgga tgtgaacgcg atgatgaccg tgatcccccg
     3241 catctccagt ccggcactga ctgcacagga aattgcagag atggacccgg cagatctcac
     3301 tgccatgtcg gttgaggttg tcactttttt gttgaagaag tcggtgcttg ccggtttacc
     3361 gacagcctga cggttgacga tctggtggct gatatcgcca ccatctttca ctggccgcca
     3421 tccgttactg acgttatgcc gctgaccgaa gtgctggaat ggcgggataa agcgattcag
     3481 agaagcgggg ccagccatga gtgacaataa cctgcgtctg caggtgattc ttaatgcggt
     3541 tgacaaactc acccgcccat ttcgatccgc gcaggccagt tcaagagaac tggctgctgc
     3601 tgtcaaaaaa tcccgcgatg caataaagca gcttgatcag gccgggagca gtctggacag
     3661 cttccgaaag ctgcaggcag aaaatcagaa attaggcgac aggctgaact atgcccgcca
     3721 gcgtgcaaat ttgctcagtc aggaactggg agcgatgggg ccgccttcgc aacgtcaggt
     3781 tgttgctctg ggccgtcaac ggctggctgt tcagcgccta gaagaacgcc agaaaaagct
     3841 gcagcagcag acggcgcttg tgcgtgcaga actttatcgt gctggtattt cagctaatga
     3901 tggcgccagt gcgacggccc gcattacccg tgaaacgatg cgttataaca ggcagctttc
     3961 tgagcaggaa gcgaggttac gacgtgtcgg ggagcaacag cgaaaaatgc acgccgcccg
     4021 gggggcttac gccaggcgtc ttgaggtaag ggatcgtatt gcaggtgccg gagccaccac
     4081 cacggctgca gggctggcaa tgggcgcacc agtgatggcg gcagtaaaaa gctataccag
     4141 catggtagat gccatgaaag gtgtggcaaa gcaggtcaat ggtctgcgtg acgataatgg
     4201 caaccgcact gcgcgttttt acgaaatgca gggtgccatc aaggctgcca gcgaacagtt
     4261 gccgatggaa aacggtgctg tggacttcgc cgcactggtt gaaggtggtg cgcgcatgaa
     4321 cgtcgcaaac cctgacgaca gctgggaaga ccagaaacgt gacctgctgg ccttcgccag
     4381 cacggcggca aaggcggcaa cagcctttga actgccagcg gatgaactgt cagaaagtct
     4441 ggggaaaatc gcccagctct acaaaatccc tacccgcaat attgagcagc tcggtgatgc
     4501 gctgaactat ctggatgata acgccatgtc gaaaggggcg gacatcattg atgtcatgca
     4561 acgcctgggc ggtgtggctg accgtctgga ttatcgtaaa gcggcggcgc tgggttccac
     4621 cttcctgaca ctcggcgctg cgcctgaggt tgcagccagt gcagcaaacg cgatggtgcg
     4681 tgaattgtcc attgccacca tgcaaagcaa gagtttcttt gaagggatga atctgctgaa
     4741 actcaatcca gaagtgattg aaaagcagat gacgaaggat gcgatgggaa ctatccagcg
     4801 ggtgctggag aaggtgaatg cgctgccaca ggataagcgc ctgtctgcca tgaccatgtt
     4861 gtttggtaaa gagtttggcg atgacgcagc gaaactggca aacaacctgc cggaacttca
     4921 gcgccagtta aaactgactg cgggcaatga tgcgctcggt tccatgcaga aagaatccga
     4981 catcaacaag gattcacttt ctgctcagtg gttgctggtc aaaaccggag cacagaacac
     5041 cttcagcagc ctgggggaaa cgctgcgcca gccgctgatg gatattctgt acacggtgaa
     5101 aagcgtcacg ggggcgttgc gtcgctgggt ggaagctaac ccggaactga cgggcactct
     5161 gatgaaagta gccgctgtgg tagctgccgt taccgtgggc ctcggcacct tagcggtggc
     5221 gctggctgca gtgctggggc cgctggcagt catccgtctg ggattctctg tgctgggtat
     5281 caaaacgtta ccttccgtta cggcaacagt aacccgaacc agcaacgcgt tgtcctggct
     5341 ggctggcgca ccactggcac tgctgcgacg cgggcttgct tcatcgggca acgctgcagg
     5401 tttacttact gcgccgttgt cgtctttgcg ccgcacggca tcactgacgg gaaatgtcct
     5461 gaaaactgta gcaggtgcgc cggttgcact tttgcggtct ggattatccg gtttacgtgc
     5521 ggttgctgtg atgtttatga atccactggc ggtactacgc ggcggactgg ctgccacagg
     5581 cacggtgctg cgtatgctcg catccggtcc actggcgatg ctgcgcgttg ccctgtatgc
     5641 cgtctctggt ctgttaggtg ctctgctcag tctgataggt cttgtggtta ctgcactggc
     5701 gggtgtggcg ctggttgtct ggaaatactg gcagcccatc accgcatttc tcggtggcgt
     5761 ggtggaagga ttcaaagcgg cggcaggtcc tgtcagtgcc gcgttcgaac cgcttaagcc
     5821 tgtgttccag tggattggcg acaaagtaca ggcgctgtgg ggctggttta ctgatctgct
     5881 gacgcccgtt aagtcgacct ctgccgaact gcagagtgca gcggcaatgg ggcgacgatt
     5941 cggggaggct ctggcggaag ggctgaatat ggtcatgcat ccgctggact ccctgaaatc
     6001 cggcgtttcc tggttgctgg agaaacttgg cattgtcagt aaagaggccg caaaggcgaa
     6061 actgccggaa agcgtgacgc gtcagcaacc tgcgacggtg aatgcagacg gtaaagtgat
     6121 gatgccatcg ggtggttttc cgtcatgggg atatggcttt gcggggatgt atgacagcgg
     6181 cgggtatatc ccgcgcgggc agtttggcat cgtcggtgaa aacgggccgg aaattgttaa
     6241 cggcccggca aatgtgacca gccggagaaa tacagctgca ctggctgccg ttgttgccgg
     6301 aatgatgggt gttgctgcta cgccagcaga gcttccaccg ttgcaccctt tggcacttcc
     6361 cgcgaaaggc ggtgaagcga tggtgagtcg tgcagccact gtgccgcccg ttcaacggat
     6421 tgaggcaccg atgcagatca tcattcaaac gcagccagga caaagtgcgc aggatattgc
     6481 gcgggaggtg gcccgccagc ttgatgaacg tgaacgcagg ctgaaggcaa aagccaggag
     6541 taactacagc gatcaggggg gatacgacgc atgatgatgg tgctgggatt gtacgtgttt
     6601 atgctgcgca ccgtgccgta tcaggagctg cagtatcaac gcagctggcg acatgcggca
     6661 aacagccggg taaaccgacg cccgtcaacg cagtttctgg gaccggacaa cgacatgctg
     6721 acgctttctg gtgttcttat gccggaggta acgggcggca ggctgtcgtt gctggctctg
     6781 gagcagatgg cagaacaggg aaaagcatgg cccctgattg aaggcagcgg cacgatttac
     6841 ggcatgtatg tgattgaggg actgaatcag actaaaacgg agtttttccg cgacggtatg
     6901 ccgcgccgga ttgagttcac cctgtcgcta aaacgcgtgg atgaatccct gtccgatatg
     6961 ttcggtgatc tcagtgcgca actgaataat ctgcagggaa cggcaacatc tgccttaagc
     7021 gatatcagta aaacggtggg agggctgctg tcgtgaattt cagctctgaa ctgcttaaca
     7081 aaggcaacaa aactccggca ttcagcatca gtattgaagg cagggatatc accactgtgc
     7141 tggacaaccg cctgatgggg ctgacgctga cggataaccg gggctttgaa gcggaccagc
     7201 ttgatctgga gctggacgac gccgatggaa aaatcgtgct gccgcgccgt ggggctgtca
     7261 ttacgctggc gctgggctgg aagggacagc cgcttttccc gaaaggggca ttcacggtgg
     7321 acgagattga acacactggc gcaccggatc gcctgactat ccgggcgcga agtgctgatt
     7381 ttcgtgaaac gctgaatacc cgccgtgaaa agtcgtggca caagaccacc gttggggaag
     7441 tggtgaagga aatagctgcg cggcacaaac tgaagatggc attgggtgaa gacttgtcgg
     7501 ataaactcgt ggagcatata gaccagacta atgagagtga cggcagtttt ttgatgcggc
     7561 tggcgcgcca gtacggtgct attgcgtcgg tgaaaaatgg caatctgtta ttcatccggc
     7621 aggggcaggg taaaagcgcc agcggtaaac cactaccggt gatcactatc acacgcaagg
     7681 acggcgacag tcaccgcttt accctggcag atcgcggagc ttacacgggc gtcattgcca
     7741 gctggttgca tacccgcgaa cccgcgaaga aagaaagcac cacggtgaag cgtaagcgca
     7801 ggactaagaa gcagaagaaa gagccggaag cgaagcaggg cgattacctg gtgggtacgg
     7861 atgaaaacgt gctggtactt aatcgcactt atgccaaccg gagcaacgcc gaacgggcag
     7921 cgaaaatgca gtgggaacgc ctgcaacgcg gcgttgcatc attctcgcta caactggcgg
     7981 aagggcgggc agatctctac acagaaatgc ctgtgaaggt cagtggcttt aaacagccgc
     8041 tagatgatgc ggaatggacc attacgactc tgacgcatac tgtcagcccg gataacggtt
     8101 ttacgaccag tctggagctt gaagtgagga ttgatgattt cgaaatggaa tgattcttcg
     8161 caatggagaa cttttaagtt tgcaaaatgg aataatgcgg tatcattatt gtgaatttag
     8221 caaaaatggg ggggggaact cgaaaaatga tgatttgccc actgtgtgga agtgccgccc
     8281 atactcgcag cagttttcag gtatcttcat tgaccaaaga gcgttacaac cagtgccaga
     8341 acattaactg cagccatact tttgttaccc atgaaacttt tgttcgttcg attgcaacgc
     8401 caaaagagtc aaatccggtt cagccgcatc caatgaaatc aggacaggtg gcgctctctc
     8461 tttgacgctg ccgccatttt gtcgccatcg ttaaaaaaca gtgcttctaa catcatgatt
     8521 ttaaaaggca taaatttcag acaacaaaaa acccatcaac cttgaaccga aatggcgggg
     8581 ttgatgggct ccacaaaatg gggacatcaa agaaaagcag tggcactaat taagactgat
     8641 gccctgcgga aaagttctgc ggttgtgcaa aaaaatttca ttttcagggc aacttcagtt
     8701 ttatcctaat cctggccata ccatgacgat gattgtccct gccagcgtca gcaggacgtt
     8761 ggcgattgcg taggtgcccg catagcccag cgcagggatg ttactgtgag ctgtatcact
     8821 gatgatctcc attgccggcg cgcaggtgcg tgcgcccatc attgcgccga acaacagtgc
     8881 gcggttcatt cgcaatacat aagcaccgaa caagaaacag ataaccacgg gcacaagact
     8941 gacaattaat ccggcaatca acatctgacc gccaatcgcg cccaggccgt tattaatacc
     9001 gctaccggcg ctcagaccaa cgcctgccat aaacaccatc aagccgaact ctttcaccat
     9061 gcttaatgca ccttgcggaa tgtaaccgaa ggtcgggtgg ttagcacgca taaagcccag
     9121 cataattccg gcgaataaca acccggcagc gttccccatg ccgaaactga atgtgctgaa
     9181 ctggaaggtg atcatcccga tcatcagccc aataacaaag aaggcgcaaa atgccagcag
     9241 gtcagtgacc tggctgtgaa tcgagataaa gccgatgcga tcggcgatgg tttttacgcg
     9301 acgggcatca ccgctgactt gtaaaacgtc acctttgtta agcacgacgt tgtcatctat
     9361 cggcatctca atctggctac gaatgacgcg gttaaggaag caaccgtgat cggtcaactt
     9421 cagttgtgcg agacgtttac ctacagcgtt atggttttta acgaccactt cttcagtgac
     9481 gatacgcatg tcgagaaggt cacgatcgaa aacttcttta ccgttacgga agctgggatc
     9541 gagtcgggca tgggcgtcgg gatagcctac caacgctatt tcatcgccca tttgtagcac
     9601 ggcatcaccg tctggatttg ccagaatccc gttacgtcga atacgttcaa tgtagcagcc
     9661 ggtttgtcga taaataccca gttcacgcag atttttgccg tcggtccagg ccaccagctc
     9721 cgggccgacg cgataggcgc ggatcaccgg taaataaacc ttacggttgg catcagtgtc
     9781 caggccacgt tcgcgggcga tttgctgggc gctggtctgt aagtcctgat gctgcaattt
     9841 cggcaagtaa cgcgcaccaa caatcaaact caccagaccg attaaatagg ttaaggcata
     9901 cccgaggctc agattatcca gtgccagtga gagctgcctg ctttccatgc cggaatgacg
     9961 cagtgtatcg ccagcaccga ccagaaccgg tgtcgacgtc atagagcctg ctaacatacc
    10021 ggccgtcagg ccaatatccc agccaaacag cttacctaac cctaaggcga tcaccagcgc
    10081 actgccaacc atcaccagtg ctaacattag gtaatttttc ccatcgcgaa aaaaaatgga
    10141 aaaaaagttc ggtccggctt cgaccccgac gcagaaaata aacagcataa agccaagatt
    10201 aagcgcatcg gtgttaatgc tgaaatgttg ttggcctaat aacagcgata cgactaaaac
    10261 gccaatggaa ttacccagtt ggatcgaacc aagtcgtaac tttccgagac atagcccaag
    10321 cgcgaggacc acaaataata acagaatgta attcccattt aacaattcgg cgacgtttat
    10381 attcacggag gctaacttct tgtttactag taagctgttg aaagaaatgg taatttacga
    10441 taatgttttt taccagaatt cagggcgcag attcattcag cgcacctaaa cgatagtaaa
    10501 gtaacaatat attttactag tgtaatcaca ttaggtatca acggctatat gaattgcgtt
    10561 ggcctatatt agcatggaat gcgaagcggc tttatcttac tgaacgccac actggcgaaa
    10621 aatgtgttcg atagacgcag tgtcaggagg aacgagtgaa acataaacaa cgttgggcgg
    10681 gggcaatctg ctgttttgtc ctcttcattg tggtgtgcct ttttctggcg acgcacatga
    10741 aaggcgcttt tcgggctgcc gggcatcctg aaatcggctt gctgtttttc attcttcctg
    10801 gagcagttgc cagtttcttt tcacagcgta gagaagtcct gaaacctcta tttggcgcaa
    10861 tgctggcggc accctgttca atgctcatta tgcggctgtt tttttcaccg acgcgctcat
    10921 tctggcaaga gctggcatgg ttactaagcg cggtgttctg gtgtgcgctg ggggcactgt
    10981 gtttcttatt tatcagtagt ttgtttaaac cacagcacag aaaaaatcag taaagccctc
    11041 aacgcgaggg cttgtcagac gatcaggcgt ccagattttc tttcacccat gcagcaaaat
    11101 cggtatagcc gccgatatgt tgctgatcga caaaaatctg cggcacggtt tctacgggtt
    11161 tacctgcctt ttgttgtaga tcttctttag tgatcccttc cgcacgaata tctacatact
    11221 gatactgaaa atcatcgcgt tcattgctca atttctcagc cagatctttt gcacgcacac
    11281 agtaagggca acccgaacga ccaaaaataa cggtttgcat tatttctctc ctcatagatt
    11341 tatgcctgta atgatcacgc taaaatgtat tcgctgaaag taggtttaac ctgttgcatt
    11401 aattgctaaa agctataact gttaaacaca atacagtgaa aagttttaga ctgaaggctc
    11461 actttgcaga gggaagcgta tgcgcgcgat cggtaaattg cctaaaggcg tgttgatact
    11521 ggaatttatc ggaatgatgc tactggcggt ggcgctgctg tcggtaagcg actccctgtc
    11581 gctgcctgag ccattttctc ggccagaagt gcagattctg atgatttttc tcggtgtttt
    11641 gctcatgctt cccgctgcgg tggtggttat tcttcaggtg gcaaaacgtc ttgccccaca
    11701 gctgatgaac cgtccaccgc aatattcacg ttcagaaaga gaaaaagata atgacgccaa
    11761 ccattgaact tatttgtggc catcgctcca ttcgccattt cactgatgaa cccatttccg
    11821 aagcgcagtg tgaggcgatt attaacagcg cccgtgcgac gtccagttcc agttttttgc
    11881 agtgtagtag cattattcgc attaccgaca aagcattacg tgaagaactg gtgacgctga
    11941 ccggcgggca aaaacacgta gcgcaagcgg cggagttctg ggtgttctgt gccgacttta
    12001 accgccattt acagatctgt ccggatgctc agctcggcct ggcggaacaa ctgttgctcg
    12061 gtgtcgttga tacggcaatg atggcgcaga atgcattaac cgcagcggaa tcgctgggat
    12121 tgggcggggt atatatcggc ggcctgcgca ataatattga agcggtgacg aaactgctga
    12181 aattaccgca gcatgttctg ccgctgtttg ggctgtgcct tggctggcct gcggataatc
    12241 cggatctcaa gccgcgttta ccggcctcca ttttggtgca tgaaaacagc tatcaaccgc
    12301 tggataaaga cgcactggcg cagtatgacg agcaactggc ggaatattac ctcacccgtg
    12361 gcagcaataa tcgccgggat acctggagcg atcatatccg ccgaacaatc attaaagaaa
    12421 gccgcccatt tattctggat tatttgcaca aacagggttg ggcgacgcgc taaaaccgcc
    12481 acgtcgatgt atgatacgcg ggcttttgac caggtctgac agagaggtgc agggtgaaaa
    12541 ttgccatatt gtcccgggat ggaacgctct attcgtgtaa gcggctgcgt gaagccgcta
    12601 tacagcgcgg tcacctggtt gaaattcttg atccgctttc ttgctacatg aacataaatc
    12661 ctgcggcgtc ttctattcac tacaaaggcc gcaagttacc ccattttgac gcagtgatcc
    12721 cgcgtattgg caccgccatt accttttatg ggacggcggc actgcgccag ttcgagatgc
    12781 tggggagcta tccgctcaat gagtcggtcg ccattgcccg ggcgcgtgac aaattgcgtt
    12841 ccatgcaact gctggcgcgt cagggcatcg acctgcctgt cacgggcatt gtgcattcgc
    12901 cggatgatac cagcgattta atcgacatgg tcggtggtgc gccgctggtg gtcaagttgg
    12961 ttgaaggcac gcagggaatt ggcgtcgtgc tggcggagac gcgtcaggcg gcggaaagcg
    13021 tgattgacgc tttccgcggt ctgaacgcgc atattctggt gcaggaatat atcaaagagg
    13081 cgcaagggtg cgatatccgc tgtctggttg ttggcgatga agtggtcgct gcgattgaac
    13141 ggcgggcgaa agagggcgat tttcgttcca atttgcatcg tggcggcgcg gcaagcgtcg
    13201 ccagtatcac accacaggag cgtgaaatcg cgataaaagc cgcgcgaacg atggcgctgg
    13261 acgttgctgg tgtggatatt ctgcgtgcta atcgcgggcc gttggtgatg gaagtgaatg
    13321 cgtcgccggg gctggaagga atagaaaaaa ccactggtat cgacatcgcg ggtaaaatga
    13381 tccgctggat cgaacgctac gctacgacag aatattgcct gaaaacgggt ggttagtcgc
    13441 aatcacatta ctgatcatgg ttttgcctgc gctttttgcg taagctgtgc cggtcttttt
    13501 atcgaaagag gttgtacaaa attatgacat cgctggtcgt tcctggtctg gatacgctgc
    13561 gtcaatggct cgatgacctg gggatgagtt tttttgaatg tgataactgt caggctctgc
    13621 atctgcccca tatgcagaat ttcgacggtg tctttgatgc caaaatcgat ctgatcgata
    13681 acacgatcct gttttctgcc atggcggaag tccgaccttc agccgtattg ccgctggcgg
    13741 cggatttatc tgccatcaat gccagttcgc tgaccgtgaa agcatttctt gatatgcagg
    13801 atgataatct gccaaagctg gtggtttgcc agtctttatc cgttatgcag ggcgtaacct
    13861 acgagcagtt tgcatggttc gtgcgtcaga gcgaagagca gatttcgatg gtcattcttg
    13921 aagctaatgc ccatcaactg ctgttaccga ctgacgatga agggcaaaac aacgttaccg
    13981 aaaactattt cctccactga taactccttt cgagcacgca gtcgctggtg cagtgactgc
    14041 gcactgcaaa attatctgct gtttttaacc ttttcttaaa gattatttca cttctcttgt
    14101 gccgatttgg ctttatcaca tagagcaaat acgcataaca atttgttaaa taccgttttt
    14161 taatccgagc tatagtctca aaccctggct aaagttattc ttgcgatgct tttatatagc
    14221 gagcagtgct ggccgggaga gagttatctt ttcttacacc gcgccgataa aaaatatgca
    14281 cgtttattgc atatctttta gtatgacaac ttttgttcgt ttgttaacga actttcagaa
    14341 ggaaagagat atgaccgcct taaataaaaa atggctttcg ggtctggttg cgggtgctct
    14401 gatggccgtc tctgtcggca cgctcgcggc tgaacaaaaa acactccaca tttataactg
    14461 gtctgattat atcgcccctg acacggtggc caattttgaa aaagaaaccg gtattaaagt
    14521 cgtctacgat gttttcgact ctaacgaagt actggaaggc aaattaatgg cgggtagcac
    14581 cggctttgac ttggtggttc catctgccag ctttcttgaa cgccagttga ctgcgggagt
    14641 tttccagccg ctggacaaaa gcaaattgcc ggagtggaag aatctcgacc cggagctgct
    14701 gaagctggtc gccaaacacg atcccgacaa taaatttgct atgccctata tgtgggcgac
    14761 gaccgggatt ggctataacg ttgataaagt taaagctgtg ctgggcgaaa acgcgcccgt
    14821 cgatagctgg gacttgatcc tcaaacctga aaatctggaa aaactgaaaa gctgcggtgt
    14881 ctctttcctg gatgcgccag aagaagtttt tgctaccgtg ttgaattatc tcggcaaaga
    14941 tcccaacagc actaaagcgg atgattacac cggaccggca accgatctgc tgttaaagct
    15001 gcgcccgaac attcgttatt tccattcatc tcaatacatt aacgacctgg caaacggcga
    15061 tatttgcgtc gctatcggct gggcaggtga tgtctggcag gcgtcaaacc gcgcgaagga
    15121 agcgaagaat ggagtgaatg tctcgttctc gattccaaaa gaaggggcga tggcgttctt
    15181 tgatgtattc gccatgcctg cggatgccaa aaacaaagac gaagcctatc agttcctgaa
    15241 ttacctgctg cgcccggatg tagtagcgca tatttccgac catgtgttct atgccaacgc
    15301 caataaagca gccacgccgc tggtgagtgc ggaagtccgt gagaacccgg gtatttatcc
    15361 tccggcggac gttcgtgcga agctattcac tctgaaagta caggatccga aaatcgaccg
    15421 tgtgcgcacc cgcgcgtgga ccaaagtgaa gagcggaaaa taatccgcag ccttagatgc
    15481 cgggcgggcg caccacaccc gccggcaatt cgcaccatca tggtgcgctt gcacacattc
    15541 aatgccggag agcagccgtg aatgacgcta tccctcgccc gcaggcgaaa acccgtaagg
    15601 cgctgacgcc gctattagaa atccgcaacc tcaccaaatc ctacgatggt cagcatgcgg
    15661 tcgatgatgt cagcctgacc atctacaaag gtgaaatctt cgcgctgctg ggcgcttccg
    15721 gctgtggcaa gtccacgcag ctgcgtatgc tggcaggttt tgaacaacct tctgccgggc
    15781 agataatgct cgatggcgtc gatttgtcac aggtgccgcc ttacctgcgc cccatcaata
    15841 tgatgtttca gtcttacgcg ctgtttccgc atatgaccgt ggaacagaac atcgcttttg
    15901 gcctgaaaca ggacaaacta ccgaaagcgg aaattgccag tcgggtccat gagatgctcg
    15961 gcctggtgca catgcaggag ttcgccaaac gcaaaccgca tcagctttcc ggtggtcagc
    16021 gacagcgtgt ggcgctggcc cgaagccttg ccaagcgccc gaaactatta ctgctcgacg
    16081 agccgatggg cgcgctggat aaaaagctgc gtgaccggat gcagcttgaa gtggtggata
    16141 ttctggagcg tgtcggcgtg acctgtgtga tggtcactca cgatcaggaa gaggcgatga
    16201 cgatggcggg acgtatcgcc attatgaatc gcgggaaatt tgtccagatt ggcgagccag
    16261 aagagatcta cgaacatcca accacccgct acagtgctga atttatcggt tcggttaatg
    16321 tctttgaagg tgtactcaaa gagcgtcatg aggatgggct tgtgctttat tcgcctgggc
    16381 tggtgcatcc actgaaagtc gacgcggatg cctcggtggt cgataacgtg ccggtacatg
    16441 tggcgctgcg cccggaaaaa atcatgcttt gcgaagagcc gcccgccaat ggttgtaact
    16501 tcgcggtggg ggaggtgata cacattgcct atctcggcga tctttcggtg tatcacgttc
    16561 gtctgaaaag tgggcagatg attagcgccc agctacaaaa cgcccatcgt catcgtaaag
    16621 ggttaccgac ctggggcgac gaagtgcgtt tgtgctggga agtggacagc tgtgtggtgc
    16681 tgacggttta aggagcaaag atgaatacac ttgaacctgc tgcccagtcg aaaccgccgg
    16741 gcggatttaa gctgtggttg tcgcagctgc aaatgaagca tgggcgcaaa ctggtcattg
    16801 cgttgccata tatctggttg atcttgctgt ttctgctgcc atttctgatt gtctttaaaa
    16861 taagcctggc ggagatggcg cgcgctattc caccttatac cgaactgatg gagtgggctg
    16921 acgggcaact gtccatcact cttaatctcg gtaattttct gcaactgacc gacgatccgc
    16981 tctatttcga tgcttatctc cagtcgctac aggtggctgc gatttcgaca ttttgctgtt
    17041 tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag taagccttcg acccgtaata
    17101 ttttattact actggtgatc ctgccgtcgt ggacctcgtt tctgatccgc gtttatgcct
    17161 ggatgggaat attaaaaaac aacggtgtgc tgaataattt tctgctgtgg ctgggggtta
    17221 tcgatcaacc gctgaccatt cttcatacca atctggcagt ttatattggc attgtttacg
    17281 cctacgtgcc gtttatggtg ctgccgattt ataccgcgtt gattcgtatt gattattcgc
    17341 tagtggaagc agcgctggat ctcggtgcac gaccgctgaa aacgttcttt actgtgatcg
    17401 tgccgctgac taaaggtggg attattgccg gatcgatgct ggtgtttatc ccggctgtgg
    17461 gcgagtttgt gatcccggaa ctgctcggtg gcccggacag catcatgatc gggcgcgtgc
    17521 tatggcaaga gtttttcaat aaccgcgact ggccggtggc cgcggcggta gcgatcatca
    17581 tgttgctgct gctaattgtg ccgataatgt ggttccacaa acaccagcaa aaaagcgtgg
    17641 gagaacacgg atgaataatt taccggtagt tcgttcgccc tggcggattg tgattttgct
    17701 gctgggcttt acctttctct acgcgccaat gctgatgctg gttatctatt cgtttaacag
    17761 ctcgaagctg gtgacggtgt gggccggctg gtcaacgcgc tggtatggtg agttattgcg
    17821 cgatgacgcg atgatgagtg cggttggttt aagcctgaca attgctgcct gtgcggcaac
    17881 ggcggcggcg atcctcggga ctattgcggc ggtggtgctg gtgcgctttg gcaggtttcg
    17941 cggatcaaat ggctttgcct ttatgatcac agcgccgctg gtaatgccag atgtcatcac
    18001 gggcttgtcg ctgttgttat tattcgtcgc gcttgctcat gccattggct ggcctgcgga
    18061 ccgcggtatg cttaccatct ggctggcgca tgtcactttc tgtacggctt atgtggcggt
    18121 cgttatttcg tcgcgtctgc gggaactgga tcgctcgata gaagaagcag cgatggatct
    18181 cggtgcgacg ccgctgaaag tgtttttcgt cattacgcta ccgatgatca tgcctgcgat
    18241 catttctggc tggttactgg cttttacttt gtcgcttgat gatctggtga tcgccagctt
    18301 tgtttccggg ccgggagcca ccacgttacc gatgctggtc ttttccagcg tgcggatggg
    18361 ggtgaatccg gaaatcaacg cccttgcaac gttaattctc ggtgcggtcg gaattgtcgg
    18421 atttatcgcc tggtatctga tggctcgcgc agaaaaacag cggatacgcg atatccagcg
    18481 tgcaagacat ggctgaagac actaaaattt gccaacctgg ctacataatg ccgcgcatgc
    18541 cgcggcattg ttttcatgga agacgaaacg ttgggatttt ttaagaaaac atcttcatct
    18601 catgctcgcc tgaatgtgcc tgcgctggtg caggtagcgg cgctcgccat tattatgatc
    18661 cgtggcctcg acgtgctgat gattttcaat acgctgggcg tgcgcggtat tggcgagttc
    18721 attcatcgca gcgtacaaac ctggagctta acgctggtat ttttaagcag tctggtgctg
    18781 gtttttatcg agatctggtg tgcgttttca ctggtgaaag ggcgtcgctg ggcgcgctgg
    18841 ctatatctgc tgacacaaat caccgccgca agttacttgt gggcggcttc gctggggtat
    18901 ggttatccgg agctgttcag cattcccggt gaatcaaaac gtgaaatctt ccatagcctg
    18961 atgctgcaga agctgccgga tatgctcatc ctgatgctgc tgttcgttcc ctcgaccagt
    19021 cggcggttct tccagttgca ataatgtgta taatcgtcgc ccctgatgat gtgaaggtca
    19081 atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt tcctgtcagt ggataacgca
    19141 gccgattcca gagcaactct ccgctaaaac cgccgatctt aaaaatctgc tcgccgattt
    19201 tccggttgag gaatggtgcg cgccggtgtc aggcccggaa caagggtttc gtaataaagc
    19261 caaaatggtg gtgagtggta gcgttgaaaa accactgctc ggtatgctgc atcgcgatgg
    19321 cacgccggaa gacctttgtg actgcccgct ttatcctgcc tcttttgcgc ccgtttttgc
    19381 ggcgctaaaa ccgtttatcg cccgtgcggg gttaacgccc tacaacgtgg cgcgtaagcg
    19441 tggcgaactg aaatacattc tgctgactga aagccagagc gatggcggca tgatgctgcg
    19501 ctttgtactg cgttctgaaa ccaaactggc acaactgcgt aaggcgctgc cgtggttaca
    19561 ggaacaacta ccgcaactga aagttattac cgtcaatatt cagccggtac atatggcgat
    19621 tatggaaggg gagacggaga tctacctgac tgaacaacag gcgctggcag agcgttttaa
    19681 tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag actaatccgg cggtcgccag
    19741 ccagctgtac gctaccgcgc gcgactgggt acggcagctg ccagttaaac atatgtggga
    19801 tctgttctgc ggtgtggggg gctttggttt acactgcgcg acgcctgaca tgcagttaac
    19861 cgggatcgaa attgcgccag aggccattgc ctgtgcaaag cagtcagccg ctgaactggg
    19921 cttaactcgt ttacaatttc aggcgctgga ctccactcag tttgccactg ctcaggggga
    19981 ggtgccggag ctggtgctgg ttaacccgcc gcgccgcggc attggtaaac cgctgtgtga
    20041 ttatctctca acgatggcac cgcgttttat catctactcc agctgtaacg cccaaactat
    20101 ggcgaaagat atccgcgaac tgcctgggtt tcgcatcgaa cgggtacagc tttttgatat
    20161 gttcccgcac accgcgcact atgaagtgct gacgctgctg gtgaagcaat aaaaaagccg
    20221 caggtgcggc ttcagattgc tgatcaggtg cgctttgttc atgccggatg cggcgtgtac
    20281 gccttatccg gcctacaaaa tcatgctaaa tcaatacatt gcagagttga tgtgggccag
    20341 ataagcgcag cgcatcaggc aatcttactt ttgtcatcag tctcaattac tgcgggaacc
    20401 actggtcatt gattttctga tatgtaccgt cagctttaat tgctgccagc gcgttattca
    20461 gtttttccag cagagctttg ttatccggac gtacagcgat gcccagaccg gtgccgaagt
    20521 attgcggatc ggtcactttt tcagtagcaa cgcccagttg cggattggtt ttcagccatt
    20581 cgtttaccac cgctgtgtca ccaaataccc catcaatacg accatttttc agatcgataa
    20641 aggcattctg ataactgtca taggagacag ttttcacttc ggggtgctga tcctgaatat
    20701 atttctggtg cgtggtgccg ttttccatcc caatacgttt gcctttcaga tcggcaaacg
    20761 ttttgtaggt atcttttttg gcaatcacga cggctgagtt ttcatagtag ggcgtggtaa
    20821 acgatacctg cttgctacgc tcgggcgtga tatccatacc ggagattacg gcgtcatatt
    20881 ttctgaattt cagtgacggg atcaggctgt cgaacgcgtg attagtaaaa gtacattctg
    20941 cctgcatttg tttgcacaag gcttttgcca gatcgatatc aaagccgaca atctcattat
    21001 tagcacctat agattcaaag ggtggatagg tggctgaaac gccaaaattg attttctctg
    21061 cggcagaagc accgaaagta aaggaagcaa gtaaagcggc aagaactaac tttttcatga
    21121 tggaactccc gtctgtcaat cttatgattt ttggccgtgt ctgcggcatg ggataacaat
    21181 gccatcaagt gaatttatat gcaataaata tgattaaata atttaaatga aataaaaaac
    21241 gacggataac ttagtgagtt gtccgtcttc attataagaa tttatgcact atgtaggccg
    21301 gataaggcgt ccacgccgca tccggcacag gcaccgtgct gatgtctgat gcgacgctgg
    21361 cgcgtcttat cagacctaca aaccccccgg cgaatgtacg cagctacatt aattccgccg
    21421 ttcgaatgcc agtgctttgc gctcgatcag acgcatcatc agcgtcagca ggccgttgac
    21481 gaccaggtaa ataatccctg ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa
    21541 caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag aggtactttt
    21601 gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg catacggcag
    21661 caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact gccactgacc
    21721 ttctgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg cactgttcag
    21781 cgataacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca gtgccggata
    21841 ctcctgcaaa gtcggaaact ggcccggccc gtaataaatc aggaagatct gcaccagcag
    21901 cggcgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca ccggcgtttt
    21961 cagcgtcagg atgatggtaa aaatcaatgc cagaatcagt gccacaatca gcgaggcaac
    22021 ggttagcgtc aggctggtgt gtagcccttt catcagttcg ggtaaatact caaacattag
    22081 ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg
    22141 agcagggtga tcaccaggta aatcgccgcc gccacaatat accaggtaaa tggttcctgg
    22201 gtacgagtag cgatgctttt tgtttgcagc attaaatcat tcacactaat caaactgacc
    22261 agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag cgcatgacgc
    22321 cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc cagcgcctga
    22381 ccagactccc actgacccac cggcaccgct ttcaacgcgc cccgaagcgt ttgcgaggca
    22441 taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacgggct aacgtcgaag
    22501 ttctcaatgt ccatctgcac tgggatctgc acgaacccaa gattgatagt gaagccatcc
    22561 gaaagcgtca gcagcagctg cgaggagcca aaatagataa acagcaccac cagaatttct
    22621 ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg acgccatttt
    22681 tccgactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg
    22741 gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt catcaggaat
    22801 tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt cactttttcc
    22861 agcgcagtgt tgagtttctg ctgcagctca gtgttgccct gacgtaccgc gatgccgagg
    22921 ccagtgccga agtaatcttt atcggtcact ttgtcgccca ccgccgccag tttcgggtta
    22981 tctttcagcc actcagtgac cactgcggtg tcaccgaaga cgccgtcgat acgcccgttt
    23041 tgcagatcca gttttgcgtt ctggtagctg tcatacggaa cggtagtgat ttccgggtgc
    23101 ttatccataa tgaatttctg gtgtgtcgtc ccgttctgta cgccgacttt tttgcctttc
    23161 agctgatcaa cactggtgta tttgccttgc tgacccacaa acagggcaga gttgtcatag
    23221 tacggggtgg taaacagcac ctgcttttca cgctccggag tgatatccat gcccgccatc
    23281 acggcttcta cgcgacggaa tttcaggctt gggatcaggc tgtcaaacgc ctggttagag
    23341 aaagtgcagg ttgcatcaat ctctttacac agcgcttgtg ccaggtcgac gtcaaaacca
    23401 acgatctggt tgtttgcatc aatcgattca aacggaggat aggatgcttc ggtagcaaaa
    23461 cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga
    23521 acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta aatgcttcgg
    23581 tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgacca ttttccatat
    23641 acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg atcacctggg
    23701 taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta atttccgggt
    23761 ccagtgcggc ggtcggttca tcgaacagca gtacctgcgg ttccatcatc aacgcacggg
    23821 caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga tcgctataag
    23881 gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga tctttactca
    23941 accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga tgcggccaca
    24001 ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag
    24061 agggggtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca
    24121 tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg gggccaagta
    24181 acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc
    24241 cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata ctggcagtct
    24301 gatagctatt gaggtcgaag atagtacctt tgacagaata attatgcaat atttctgctt
    24361 taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac gggggcggtg
    24421 gtcggcgagc ataaatgtcg gcattcctca cgaaatgccg gacaatttac ggggtttatt
    24481 ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc cgtggcatga
    24541 acgctgccgc ctaagtagcg tacatcgtcg atcacccaac actggccttc ctgaatcatt
    24601 aacacttcat cctgccagct ctgatcaccc tgttttagat ccacacgcag aggaatattt
    24661 cgcgcatcac gattcgggat tgttgaggca ctggcaacac gggcgctctc tggcaaggtt
    24721 gtgcgactgg agaatggatc gttggtcagt agttcccgat ggttattatc ccgggaggca
    24781 tcgctaagca gtgtcgccag tttgtcgctc agataagggc gcaaggcggt gatgtcgttg
    24841 ctgcggtgca aaatgcggta gtcataaaat tgctgggcca cgttacccgg gcctccttca
    24901 acgcaaggac cactgcgtgt gccgttatct ttataagctg gagtgactgt ggtgcaggca
    24961 ctgaggagca gtgcgcaggg gataagcatt gtcaatttgc tgtagcgcat aatgatttcc
    25021 ttataagcga tcgctctgaa agcgttctac gataataatg atatcctttc aataatagcg
    25081 tatcagtctg ataatgcttt tgagatcgaa ggcttagcaa acaaggagat cgatcatgca
    25141 attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg gtgtggtgac
    25201 cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta tccgcgatat
    25261 cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg agatcgcctt
    25321 tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtcgtcg gtattgatat
    25381 cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca gcggtacggc
    25441 ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct gttattggca
    25501 gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga tacccgacgc
    25561 caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac cgcagatgat
    25621 tttgatagct cgctggcgac actgaccgat aagcaggtca gctcgcatta tctggtccct
    25681 gcggtaccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa
    25741 ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct
    25801 attggcattg agctggaaaa ccgtggctgg caaaaatcgg ccggagtgaa atattttgcc
    25861 ccgtttgaac cggcacagat tcaggcgctt attccactgg cgaaagatat tattgcccgt
    25921 tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca gcgcaaagac
    25981 gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg tgcctggccg
    26041 gatgcgcagc gggttaactt ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc
    26101 gcgtcattgc tggagctttt ggcgcgctac ggttatgacg ttaaacctga tatgacaccg
    26161 cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac gttatataac
    26221 ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa atacgggcag
    26281 gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc gataccttca
    26341 tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat atccagcgta
    26401 aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt tgctgacttg
    26461 cggaaacgct ccatgctgcg ggcgatcata tccagcatcg ggtaggggac ggaacgaata
    26521 cgacagtcaa tattcaactc gtcgatcagc ttctgcacga tgctgcacag tgtgcgatgc
    26581 tcgccgttgg tgatgttgta cacacgcccg gaaggtagct tatcgcaggc ttcctggctt
    26641 gccagccaca ttgcgtgcac ggcattttca tagtaggtca tatcgaccag cgcactgccg
    26701 ccatgcggta acagaatact gccgtagtgg tgcatcatat gcgccagacg gggaataaag
    26761 actttatcgt gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga
    26821 ttcgcctgcg aaaacatatt gatcacttct tcgctggctg ctttgctgcg ggcaaactcg
    26881 ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg ataatcgaag
    26941 tacagggagg gggaagagat atgaataaag ttacgcacac cccaggcgac agcccattca
    27001 cccaggcggc gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt cccccagggt
    27061 gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg cgagcatcac tttagcttgt
    27121 gatgaaacca gctcggtcag atccgccgga acaaactctg cgcccatttt ttccagcaat
    27181 ttgcccattg cctcgttgcg accggtcgct cgcacgctga tgcctttctg gcataaaaac
    27241 tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt catatcaatc
    27301 cactgttgtt gagaaaataa cgtgcgcatt cttccgtgat ttcccccatg atgcaatggg
    27361 aaacatgaaa gaataacgca ggttttgtcg attaatctgt gctttgttct gccagtctgg
    27421 cgatttgttt tgccattccg cgaaaaataa acagatgcgc ggggatcatc aataaccagt
    27481 aaaacagccc cggcataccg tgcggatgcc agaaagcgcg gacatcgata gtacgatagt
    27541 cgcctttatc ttccaggcta aaacacagtc gtcccagccc cggcgctttc atgccaaata
    27601 acaacgtaag ttgtttttcc ggttcaacga caatcacttt ccagctatct accgcatcgc
    27661 cagtctgtaa atattcgcgc tccgggcggc ctttcgccag cttatgaccg atcgcgcggt
    27721 ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct ttaccgccga
    27781 tttggttcac tacctgccat aaagcagcaa ggctggcgga cgttttaacg gtaaaccccg
    27841 cctgtttggc aaaataaccg tactccggtc gccagcgggc aaaggcctga gcgtcgtagc
    27901 cccagtcgct ggagttgacc agtttttcct cctctttcaa cgtgctacgt actgcgtcat
    27961 cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc ggtatcatcc gccagcagat
    28021 cgtgtttcag cccctgaatc aacgccctgg cggtggtggg cggtacggaa gtaatcacat
    28081 tgagaaacca caccgaaatc cagcgggtgg ggagggggat ggggatcaac cagcggcgct
    28141 tgccgctcac cgccataaaa tgttcaaact gttgctgata actgagcacc tctggtccgg
    28201 cggcttcgaa gatgcggtgt tcgctggccg gatgatctaa caacgccacc agatagtgca
    28261 gcaagttttc cagcgcgatg ggcgtggtgc gtgaacgtac ccagcgtggc ggcgttaaca
    28321 ctggcaggtt gtagaccata tcgcgcatga cttcgaacgc cgctgaacct gcgccaacga
    28381 taattccggc tcgaagttcg gtcacaggta cattcgcttc acgaagaatg tccgccgtag
    28441 cctgacgagc acgcagatga tccgactgct catgtggcgg ggcctgcaac gaactgagaa
    28501 agattaattg cttaactggt acttcacgta gcgcatcgcg gacgttgaga gccacctggc
    28561 gctcctgagc gataaaatcg ccgccttcgc ccatgctgtg caccagaaaa tagacggtat
    28621 cgatatcctg caacagggcc ggaaggttat ccggccagct gagatcgact ttatggcaac
    28681 tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct
    28741 gatgcccttg ctggctgagt gtgcgcacca gatgctgacc aatgtagcca ctggcaccga
    28801 gaactaaaat gcgttgcggc acgtctctct ccttaacgcg ccaggaatgc gcgccagtgg
    28861 gcggcgactt ccgccagttg ttcgcgcgag acgtcaagat gcgtcaccag gcggacaatc
    28921 ggcgaggcgt taatcagcac gtttctcact ttcatgtatt cgcctaacgc ggcagcattt
    28981 tcttccccga cgcgaacaaa cagcatatta gtgtcctgac gcatcacatc cgcgcctgct
    29041 tcacgcagtt gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa ccgtgcgacg
    29101 ttatttttca gggcatacat cccggcggca gccagaatcc cggactgacg catcccgcca
    29161 ccggccattt tccgccagcg gatggcacgt ttaatgtaat cacgattacc gacgagtaat
    29221 gaaccgacag gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc acaatattgc
    29281 gtgatctctt tcagttcgca gccgtaagcc accacggcat taaagatgcg cgcaccgtca
    29341 acatgcagcg ccagattgcg ctcgcgggta aattcccatg cttctttcag gtattcacgc
    29401 ggcagcactt tgccgttgtg ggtgttttcc agactgagta atttggtgcg ggcgaaatgg
    29461 atatcgtcgg gtttgatttt catcgccact ttatccagcg gtagcgtgcc gtcggcagcc
    29521 gcgtcgatgg gttgcggctg aatactgccc agcaccgctg cgccaccggc ttcaaacaga
    29581 tagttatgcg cggcctgacc gacaatatac tcttcgccac gttcgcagtg actgagcaga
    29641 gcgaccaggt tggcctgagt gccggtaggc agaaaaatgg cggcttcttt accggaaagc
    29701 tctgctgcat agtcctgcag agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca
    29761 accggggcgg ccatcatcgc ttcgagcatg gcgcggctcg gtcgggtaac ggtatcactg
    29821 cgtaaatcaa tcatggcatg tccttattat gacgggaaat gccacccttt ttaccttagc
    29881 cagttcgttt tcgccagttc gatcacttca tcgccgcgtc cgctgatgat tgcgcgcaac
    29941 atatacaagc taaatccttt ggcctgttcg agtttgatct gcggtggaat ggctaactct
    30001 tctttggcga ccaccacatc caccagcacc ggaccgtcga tggagaaggc gcgttgcagg
    30061 gcttcatcaa cttcagacgc tttttctaca cggatacccg taatgccgca cgcttcggca
    30121 atgcgggcaa agtttgtgtc gtgtagttcg gtgccgtcag tcaaatagcc accagctttc
    30181 atctccatcg ccacaaagcc cagcacgctg ttgttaaaga cgacaatttt cactggcagt
    30241 ttcatctgca ctactgagag gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac
    30301 atggcgacca cctgacgttc tggctctgtc gcctgcgcac ccagcgcctg cggcatggcg
    30361 ttagccatcg aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt catttttaga
    30421 taacgtgccg cccacaccgt tggcgtacca acgtcacagg tgaaaatagc gtcatcggcg
    30481 gcaaaatgac taatttgctg cgccagatat tgcgggtgaa tggctttctc gctcggttta
    30541 gctaaatcgt ccagcccttt gcgggcgtcg cggtaatctt ccagcgcttt atccagaaac
    30601 ttgcgatcgg ctttttcttc caccaatgga agcaatgcac gcagagtcga cttgatatcg
    30661 ccgaccagtg ccatatccac cttgctgtga gcgccgatgc tggctgggtt gatatcaatc
    30721 tgaatgattt tggcatcggt cgggtagaag gcgcggtagg gaaattgcgt gccgagtagc
    30781 actaacgtgt cggcgttcat catggtatgg aaacctgacg agaagccgat taacccggtc
    30841 attccaacat catacggatt atcgtattcg acatgttctt taccgcgcag ggcatgaaca
    30901 ataggcgctt taattttccc ggcaaactca actaactctt tatgcgcccc cgcgcagccg
    30961 ctgccacaca tcagggcgat attgctggaa taacgcagca gttgcgccag tttgcgtaac
    31021 tcttcttctt ccggcgtcac gactggttgt ggcgcatgat accagtgcgt ggttgcccct
    31081 tctggcgcag gttttaacgc cacgtcgcct ggtaacacga caaccgaaac gccacggtta
    31141 agcaccgctt tgcgcatggc aatcgccagt acttgtggga tctgctccgg gctggaaacc
    31201 agctcgcaat agtgactaca ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag
    31261 ccgctgccaa tttcgctgga gggaatatga gcggcaatcg ccagtaccgg aacgtgattg
    31321 cggtggcaat caaacaggcc gttgattaag tgcaggttgc cggggccgca cgatccggcg
    31381 cagaccgcca gttctccgct aagttgtgct tcagcgccag cggcaaaggc cgccacttct
    31441 tcgtggcggg tggacatcca ctcgatggtg cccatgcgat taagactgtc actaagacca
    31501 ttcagagagt cgcctgtgac tccccagatg cgtttcaccc ctgccgattc gagtgttttg
    31561 gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt gataacggta
    31621 acaagtttag ttcatctgac ggagggggaa gggatgggag agaaaggagg cactaacggt
    31681 taaatagccc gatgaaagga atatcatcgg gcataaggcg attatgcgag aaccaaatcc
    31741 ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc gtcggtcagc
    31801 gtcattgtac tgctcaccgt atattcaccg gaaaccactt ttgtcttaca gcagccgcaa
    31861 acacccgcac ggcaggcagc gacaaccgga acgttattgc tttccagcgc ctccagtagt
    31921 gtggtgccaa ccggggcgta aaattctcgt gccggttgca gtttggtgaa tttcagaccg
    31981 ctggtcgccg cttttgctac tggggtgaag aatttctctt taaagaaacg cgtcacgccg
    32041 agcgctttca cttcctgctc tacccaatcc atatacggag ccgggccgca ggtcatcacg
    32101 gtacgtgaag ctaagtcagg tacacctgcc agcagttctc gagtgagacg accagcgata
    32161 aagccttcgg taacgttatt ttctgccacc agcgttaccg gatagttacg ccactcatcg
    32221 gcgaaaataa catcctgcgg cgtatgcacg ttgtagatca cccgcacatc ggcctgtgga
    32281 cggttcttcg caagccagcg acgcatcgac ataatcggcg tgacgccgca gcctgccgcc
    32341 agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat cgcgtccgaa
    32401 agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc gacaccgtca
    32461 tcaatccgcc gcacggtcag ggtgatatat tcactcacgc ctggcgtgga ggaaatggtg
    32521 taagcacgca gcgtttccgc tgagtttcgc acgctgacca gtgcatattg cccggcgcga
    32581 tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt ttcttgcgta
    32641 atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat cgacaaactc
    32701 cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga
    32761 atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa accaggtgca gtcgggccgg
    32821 tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacgatc gctttctgtt
    32881 caaaccagga gagcaccagc gacagcggca gatcgttcac accacagccc agtttctctg
    32941 ccagagtgac agccagaata atcgctgagt aagcatcgtt acattgacct gcatctacca
    33001 gacgcggcag accttcgata tcgccaaact caagtttgtt aaagcgatat ttaccacagg
    33061 cgagggtcag gatcaggcag tcatccggca cgatggtggc gaaatcggtg aagtagtggc
    33121 gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt ttttcacggc
    33181 tcaccagatc aatcagcgta tcagcagcgc caagcagcgt ctggcgacca aaacccacgg
    33241 tgataaggtg cggaatttcg ctgtacggga agcctgccat ctgttgcgcc tgggtgataa
    33301 ccgcagagaa atcatcaccg tccagatgac gcacgccagg ccagccaaca atgctgcggg
    33361 tccagatacg atcgtcataa gcgcttacgg ttgggtcgat gatgcagttc gaggtcatca
    33421 cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagccg ctgccgtagt
    33481 taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc
    33541 cgtgggtgta gacattaacg cccgtgcctt cggtctgttc cagcaggttg tagagatctt
    33601 tgagatcgtg accggaaatc agaatgcatt tacccgccgt cgctttgacg ttgacctggg
    33661 ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc atcactttga
    33721 agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg gcaggccagg
    33781 tccccagcca cgccatgatt ttatggtact gggcataaat atcgttgtcg tattgaccga
    33841 gaacatgcgc gtgttccata taggccgccg cacctttcag gccatacagg cacagcagac
    33901 gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta aattctgctg
    33961 cctgacgttg cagctcgccg agatcatcgc tcaccagttg caggtcagcc atcgggttat
    34021 cgacgcgcgc gttggcatct acagccaggc attgtgcttt cagcgcctcg cgcaaggcaa
    34081 tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa gttaacgttg gtcagggttg
    34141 agaaaaaggc acgtggcgcg aagctgtcaa catcgtggtt gatgatgccg tattcacgcg
    34201 cttttaccgc ccaggcagaa agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag
    34261 aagtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt cctgccggag
    34321 tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa gttatattta
    34381 atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt tcatgcaact
    34441 ttaagggaga ttgatttagc gcaattttgg cggcagggat ctaccgccag agaggtatta
    34501 cgcagagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac cgtgaacaat
    34561 tgccgccggg accatatcca gcccgccagt acgttgaaga acgggcaggg tgaaatccat
    34621 tgatgtggca ccgcataagc ccagtgcagt agagcggctg cggcgaatca gcccagggat
    34681 caacataata gcaatcagtt cacgggccag atcattaaaa aacgccgcgc tcccgattac
    34741 cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga aaccggaggc
    34801 cattgccagc gcggtattga tggggagatc aagaataaag gcgttaatta aaccaccaat
    34861 taatgaactg acaaccacca ccacggcgac aatcattccc cggcgattaa ggacaatctg
    34921 ctttaaggtc atgccattat tgcgcaactg aataccaacg aggaaaagta gcaaaattaa
    34981 cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca gaccaatggc
    35041 aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg caatacgcga
    35101 cgggagtttt tcttgctgat ggtggttgcg ccacggcagg cctcgctcca gccacatcag
    35161 ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat gcagaatcgc
    35221 caacaggtta ctggcgaggt tatcgagaaa cgccagactg atacccataa aaaagagaat
    35281 aaggtaaacc atccagctta atagctgatt aataactttt aacgcagctt gttggcgaag
    35341 cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag aaaacatgaa
    35401 aacccagtcc ttgcaaagat gaagtcgaaa tgcgcgatga cacactactg aaagcggaag
    35461 gacgagtaaa gttgcaatta aaaggaaatg ttatgcataa ggagcagtag agtattcgtt
    35521 ttcatttaaa gatattcttg cgctttaatt acaaactgca ccgatgttgg tggcgtcaaa
    35581 atcgccgagg cgttccctga aggccggggc agcccacatg gatgtgggct gagggcgcgt
    35641 tttacaggga tgttacctcg cgcccgaccc ggtagccgta agggataagt cgagggcacc
    35701 gcgcagcggc gattttgttc gccagagccc gggggggcag ggggcggcgg cgattggccg
    35761 ccccctgcgc gctccttgcg ccagtggcaa tatgtggctt agctcatgaa aggagcgcaa
    35821 caagatgatg aatcaacata taacaacatc ttaaaaaaag cctgacatta cgccaggcct
    35881 tctgcgttaa ttaatcacgc ttttccagca gggtccggta aatcagacca ccgataatgc
    35941 cgccgacaat tggcaccacc cagaagaacc acagttgttc taatgcccag ccgccctgga
    36001 agatagcaac cgcggtgctg cgcgccgggt taacagaagt gttagtcacc ggaatactaa
    36061 ttaagtgaat cagggttaag gccagaccaa tagcgatcgg cgcaaaacct gccggcgcga
    36121 atttggcggt tgcgccgtgg atcaccaaca ggaaacctgc actcaatacc agttcaacta
    36181 ccagcgcgga aagcatggaa taaccgcctg gtgaatgctc gccataaccg ttagaagcaa
    36241 aaccgctggc tgccgcgtca aaacccgttt taccactggc aattaaatac agcagcgccg
    36301 ctgcaacaat accgccgaca acctgggcaa ttacgtagcc aacgacttct tttgccggaa
    36361 aacgtccgcc agcccataaa ccaatagtga ccgccgggtt aaaatgacca ccagaaatat
    36421 gaccaacagc aaaggccatc gtcagaacgg tcagaccgaa cgccaacgcc acgccggcaa
    36481 aaccaatgcc taattccggg aagcctgcgg ccagtacagc actaccacag ccaccaaaaa
    36541 caagccagaa agtaccaaaa cattcagctg ctaattttct gaacatatcc acctcaatta
    36601 aaaattgacc ctgtgaaaaa tatggtcgtt ttatagggtc gtcgtaaaaa aatgacgacg
    36661 gaaataatgc gcggctattt taaaaacgaa ggcgagtcat tcaccagata aataaatcca
    36721 gtaaatttga tttagggcaa cagcgggttg ccccatatag tcatttgtct gattgacagt
    36781 gtagtgcacg caaaagattt aatcctttag gcgtaataaa aaataatata tcatgctaat
    36841 tatttgattt tgttgttttt gcagacttat cagcaagagg gagtataacg cgattattcg
    36901 ctcatttttc agacatttgc catgcttaaa tgtgatgtca tcacgtatta gcaaggcctt
    36961 tcccgttata ctgccagcgt aaaggataag tcacatattt ctggagggga tatgattctt
    37021 gagcgcgttg aaattgtggg ttttcgcggt atcaaccgtt tgtcgttgat gctggaacaa
    37081 aacaacgtcc tgattgggga gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc
    37141 ctgctgctat cgccagaatc agatctctac cattttgagc gcgacgattt ctggttcccg
    37201 ccgggagata tcaacgggcg agaacatcat ctgcatatta ttttgacctt ccgcgaatcg
    37261 ctgccaggcc gacatcgggt tcgccgttat cggccgctgg aagcgtgctg gacgccatgc
    37321 accgatggct atcaccgtat tttttatcgt ctggaagggg agagtgcgga agacggcagc
    37381 gtgatgacac tgcgcagttt tctcgataaa gacggacatc cgattgatgt cgaggatatt
    37441 aacgatcagg cacgccatct ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt
    37501 tttatgcgcc gtattcgtaa cggcacggtg ccaaatgtcc ctaatgtgga agtcaccgcg
    37561 cgccagctcg atttcctcgc ccgtgagtta tcctcacatc cgcaaaatct ctctgatggg
    37621 cagattcgtc agggactttc cgcaatggta cagctgcttg agcattattt ctctgagcag
    37681 ggggccggac aggcgcgata tcgtttaatg cggcggcggg ccagcaatga gcaacgaagc
    37741 tggcgctatc tggacatcat caaccggatg attgaccgac ctggagggcg ctcgtatcgg
    37801 gttattttgc tcggcctgtt tgctacttta ttgcaggcaa aaggcacatt gagactggat
    37861 aaagacgcgc gtccattgtt gctgatcgaa gatccagaaa cccgtttaca ccccattatg
    37921 ctttcagttg cctggcatct gttgaatctt ctgccattgc agcgcattgc caccaccaac
    37981 tcgggtgagt tgctttcgtt aacgccggta gagcatgttt gccgactggt acgtgagtcc
    38041 tcgcgcgttg ccgcctggcg tctggggccg agtggcttga gtaccgaaga tagccgacgc
    38101 atatcctttc acattcgttt taaccgtccg tcatcgctgt ttgcacgctg ctggttgctg
    38161 gtggaagggg aaacggaaac ctgggttatc aatgaactgg cgcgtcagtg cggacatcat
    38221 tttgatgccg aagggatcaa ggtcattgag tttgcccagt ccgggctaaa gccactggtt
    38281 aaatttgccc gccgaatggg gattgaatgg catgtactgg tcgatggcga tgaagcaggg
    38341 aagaaatatg ccgctacggt acgcagcctg ttgaataacg atcgggaagc cgaacgagaa
    38401 catttaacgg cgttaccggc gctggatatg gaacatttta tgtatcgcca gggattttcc
    38461 gatgtgttcc accgcgtggc gcaaatcccg gaaaatgtac cgatgaatct acgcaaaatt
    38521 atctcgaaag cgatccatcg ctcttccaaa cccgatcttg ccattgaagt ggcaatagag
    38581 gcaggacgtc gtggtgtgga ctccgtaccg acgctgctga aaaaaatgtt ctcacgcgtg
    38641 ctgtggctgg cgcgcggtcg cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga
    38701 atagcgtcga gcatctcata gcgccgacgg tattcagccc gttttttact ggcgatttcg
    38761 gcaatctctt ttcgtgctat ctgtgctgga aggcggtaat ggcgttcagc atcacatacg
    38821 ccgccaaccg attcccagaa agcgttgtaa tcagcgtgga tcttgccttc tttatcgcga
    38881 taacgcaggc tgcggtaaat atgcgtttca ttgctgacgg caataatctg ctctacctgc
    38941 aaacgttggg caaacagaca ggccgcttcc atcacgaggc gtttgggaaa tagcccgtgg
    39001 caggctttcg tcgcattctg gatttcctga tgtggaattt cccattttgc gccttgcagt
    39061 ccgccaataa acatcgttct tttcccctga tattcacaca gggtaaacgt gatctctgcc
    39121 agaggaatac cttcgctgtt gcgaaacagg attgtgctgt caccttcttt atccattgag
    39181 atcatcatgg tcagcttaag cgtgaactgc tcgccgtttt tgccttccag cttcgccagt
    39241 tgcagcccgg gggtattcaa atataagctg aattcttccg ccgacataca tccgcggagt
    39301 aacgcataat ggtaacgtaa cgcctccagc aattgcttac ggctaagatt cgccgcaagg
    39361 taagggcgat gcagacgcac aggcagtcgc ggctggcgcg ttaacaacac attgagatta
    39421 ggccagtggg aaagttcgtt catccactca acgcttaaac gcggcataat caacgagcgc
    39481 agcaaaaatt tctggcgaaa actacggcga tgccagaatt tacccggccg acactgtcca
    39541 cgtgccagac taagaaaaag tgacaggctg ctgagagatt cagatggcgt aaaggtccgt
    39601 tcagttagct gcgacatatt catgaaatca ttggttatac atgacgtcga tttcaccatt
    39661 gcgtatctta accaaacatc aatagtgtga ttactaacgt aaattttagg gttttgttga
    39721 tatttcgttg aagttaatga cccggattgg catatggagt attcagaata tttatgaaaa
    39781 agcggaaaac cgtgaagaag cgttacgtta ttgcgctggt gatagtcatc gccggactga
    39841 ttacgttatg gagaattctt aacgcacccg tgccgactta tcagacactg attgtgcgcc
    39901 ccggtgattt acagcaaagc gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg
    39961 atgtgggcgc gcaggtcagc ggtcagttga aaactctgtc ggtggcgatt ggcgataaag
    40021 taaaaaaaga ccagctttta ggggttattg atcctgaaca ggctgaaaac cagattaagg
    40081 aggtcgaagc aacgctgatg gagctacgtg cgcagcggca gcaggcggaa gcggagctga
    40141 aactggcgcg ggtgacgtat tcccgtcagc aacgtctggc acaaacgcag gcggtttcac
    40201 agcaggatct cgacaccgcc gcgacggaga tggctgtgaa acaggcgcaa attggcacca
    40261 ttgacgcgga aatcaagcgc aatcaggctt ctctcgatac ggctaaaacc aatctcgatt
    40321 acactcgcat cgttgccccg atggccgggg aagtcacgca aatcaccact ctgcaaggcc
    40381 agacggtgat tgccgcacaa caagcaccga acattctgac gctggcagat atgagcacca
    40441 tgctggtaaa agcgcaggtt tctgaagcgg atgtaatcca cctgaagccg gggcaaaaag
    40501 cttggtttac ggtgcttggc gatccactga cgcgctacga ggggcaaatc aaggatgtac
    40561 taccgacgcc ggaaaaggtt aacgacgcta ttttctatta cgcccgtttt gaagtcccca
    40621 accccaatgg tttgctgcgg ctggatatga ctgcgcaagt gcatattcag ctcaccgatg
    40681 tgaaaaatgt gctgacgatc cctctgtcgg cgttaggcga tccggttggc gataatcgtt
    40741 ataaagtcaa attgttgcgt aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac
    40801 gtaacgatac cgatgttgag attgtcaaag ggctggaagc gggcgatgaa gtggtgattg
    40861 gtgaggccaa accaggagct gcacaatgac gcctttgctc gaattaaagg atattcgtcg
    40921 cagctatcct gccggtgatg agcaggttga ggtgctgaag ggcatcaccc tcgatattta
    40981 tgccggtgag atggtagcga ttgttggcgc ttcaggttcc ggtaaatcga ccctgatgaa
    41041 tattctcggc tgtctggata aggccaccag cggcacctat cgcgtcgccg gtcaggatgt
    41101 tgccacactg gacgccgatg cgctggcgca actgcgccgc gagcatttcg gctttatttt
    41161 ccagcgttac catttacttt cgcatttaac cgccgagcag aacgttgaag tacccgccgt
    41221 ctatgccggt cttgagcgga aacagcggct gcttcgcgcc caggagttac tgcaacgact
    41281 ggggctggaa gaccgtacag agtattatcc ggcacagctt tcgggtggtc agcaacagcg
    41341 cgtcagcatc gcgcgggcat tgatgaacgg cggtcaggta attcttgccg atgaaccaac
    41401 cggcgcactg gacagccatt ctggcgaaga ggtgatggcg atcctgcatc agctgcgcga
    41461 tcgcgggcat atggtgatta tcgtcaccca cgatccgcag gtcgctgctc aggccgagcg
    41521 ggtgatcgaa attcgcgacg gcgaaattgt gcgcaatcct cccgccattg aaaaagtgaa
    41581 tgttgctggc gggacggagc ctgttgtcaa cacggtgtct ggctggcggc agtttgtcag
    41641 cggttttaac gaggcgctga cgatggcatg gcgggcgctg gcagcgaata aaatgcgtac
    41701 tttactgacc atgctgggga ttattatcgg tattgcgtcg gtggtttcca ttgtcgtggt
    41761 gggtgacgcc gccaaacaaa tggtgctggc ggatattcgt tctattggta cgaatactat
    41821 tgatgtctat cccgggaaag attttggcga tgacgatccg caatatcagc aggcgctgaa
    41881 gtacgacgac ttaatcgcca tccaaaaaca accgtgggtc gcctcagcca cacctgccgt
    41941 ctcgcaaaac ctgcgcctgc gttataacaa tgttgatgtt gctgccagtg ccaatggcgt
    42001 gagcggcgat tattttaatg tctatggcat gaccttcagt gaaggaaaca cctttaatca
    42061 ggagcagctg aacggtcgtg cgcaggtcgt ggttctcgac agtaatactc gccgccagct
    42121 tttcccccat aaagcagatg tggttggcga ggtgattctg gtcggcaata tgcccgccag
    42181 agtcattggt gtggcggaag aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt
    42241 ctggctacct tacagcacga tgtccgggcg agttatgggc cagtcgtggc tgaactccat
    42301 tactgtcagg gtaaaagaag gatttgacag cgccgaggcg gaacagcaac tcacgcgttt
    42361 actttcactg cgccacggaa agaaggattt ctttacctgg aacatggacg gcgtcttgaa
    42421 aactgttgaa aagaccacac gtactttaca actgtttctg acgctggtgg cggtgatttc
    42481 gctggtggtg ggcggtattg gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac
    42541 gcgggaaatt ggcattcgca tggctgtagg tgcgcgagca agcgatgttt tgcaacagtt
    42601 cctgatcgaa gccgtactgg tttgcttggt cggtggcgcg ttgggaataa cgctgtcact
    42661 gttaattgct ttcaccttgc agcttttctt acccggctgg gagattggtt tttcaccgtt
    42721 ggcactgctg ctggcgtttc tctgctcgac ggccaccggg attttatttg gatggttacc
    42781 cgcacgaaat gcggcacgac tggatccagt agatgctctg gtacgagagt aatttttgag
    42841 ataaaaatgc cagccgatcg ggctggcatt ttgcctttag gatgtacaca atgagacaga
    42901 agagctatgc gactgccgct tctacttcga cgggcacaat aacactggcg tgattgcctt
    42961 ttggcccctg gtggacatca aactgaacgg attgtccagc ttttagcgtt ctgtaaccat
    43021 ccatctgaat ggtggaataa tgagcgaaaa tatcttcgcc gccgccttca gggcagatga
    43081 aaccaaaccc tttggcattg ttgaaccact taacagtacc cttttccatg cttcgacatc
    43141 cttcgcaaat cttatacaag taagatggaa taaaccgggg tcagagaggg ggctgttcaa
    43201 aacctcgcca actctagaaa tacaatttag agaattaggg cgagccgtca agcgtttgac
    43261 aggggccagg gggcaggtat gaatcaaaaa tttgaagcag ttaacgctat tgacaggaat
    43321 gtgacagatg tcgctgatgc caacgataga tgatagttat ctatcatgtg gagtagattg
    43381 gtcaggcaaa taagctcttg tcagcggcag ggcgttctgc cgataaccgt aaccgaagat
    43441 gataactgac aatgggtaaa acgaacgact ggctggactt tgatcaactg gcggaagaaa
    43501 aagttcgcga cgcgctaaaa ccgccatcta tgtataaagt gatattagtc aatgatgatt
    43561 acactccgat ggagtttgtt attgacgtgt tacaaaaatt cttttcttat gatgtagaac
    43621 gtgcaacgca attgatgctc gctgttcact accaggggaa ggccatttgc ggagtcttta
    43681 ccgccgaggt tgcagaaacc aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc
    43741 atccattgct gtgtacgcta gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc
    43801 ctatgctcaa tcaagaactg gaactcagtt taaatatggc tttcgccaga gcgcgcgagc
    43861 accgtcatga gtttatgacc gtcgagcact tgttactggc gctgctcagt aacccatctg
    43921 cccgggaggc gctggaagcg tgttctgtgg atttggttgc gctccgtcag gaactggaag
    43981 cctttattga acaaaccaca cccgttctgc ctgccagtga agaggagcgc gacacacagc
    44041 cgacgctgag ttttcagcgt gtactgcaac gtgcggtctt ccatgtccag tcctccggtc
    44101 gcaatgaggt aaccggtgca aacgttctgg tcgctatctt tagcgaacag gagtcgcagg
    44161 cggcatatct gttgcgtaaa catgaagtca gccgtctcga tgtggtgaac tttatctctc
    44221 atggcacgcg taaagacgag ccgacacagt cttctgatcc tggcagccag ccaaacagcg
    44281 aagaacaagc tggtggggag gaacgtatgg agaatttcac gacgaacctg aatcagcttg
    44341 cgcgcgtggg cggaatcgac ccactgattg gtcgtgagaa ggagctggag cgtgctattc
    44401 aggttctctg ccgtcgccgt aaaaacaacc cgctgctggt gggggaatct ggtgtcggta
    44461 aaaccgcgat tgcggaaggt cttgcctggc gaattgttca gggcgatgtg ccggaagtga
    44521 tggctgactg tacgatttac tctctcgata tcggttctct gttagcgggc actaaatatc
    44581 gcggcgactt tgaaaaacgt tttaaagcgt tgctcaagca gctggagcag gacactaaca
    44641 gcatcctgtt tattgatgag atccacacca ttatcggtgc gggtgcagcg tctggtggcc
    44701 aggtcgatgc ggctaacctg atcaaaccgt tgctctccag cggtaaaatt cgcgtaattg
    44761 gttcgacaac ctatcaggag ttcagcaaca ttttcgagaa agaccgtgct ctggcgcgtc
    44821 gcttccagaa aattgatatt actgaaccgt cgatcgaaga aactgttcaa atcatcaatg
    44881 gcctgaaacc gaagtatgaa gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg
    44941 cagcggtaga gctggcggtg aaatacatta acgatcgtca tctgccggat aaagccattg
    45001 acgttatcga cgaagcgggc gctcgcgcac gcctgatgcc ggtaagcaaa cgcaagaaaa
    45061 ccgttaatgt ggcggatatt gagtccgtgg tggcccgtat tgcgcgcatt ccagagaaga
    45121 gtgtttctca gagtgaccgc gataccctga aaaacctcgg cgatcgcctg aaaatgctgg
    45181 tcttcggtca ggataaagcc attgaggcgc tgactgaagc cattaagatg gcgcgtgcag
    45241 gtttaggtca cgaacataaa ccggttggtt cgttcctgtt tgccggccct accggggtcg
    45301 ggaaaacaga ggtgacggta cagctttcga aagcgttggg cattgagctg ctgcgctttg
    45361 atatgtccga gtatatggaa cgccataccg tcagccgtct gattggtgcg cctccgggat
    45421 acgttggttt tgatcaggga ggtttgctga ctgatgcggt catcaagcat ccacatgcgg
    45481 tgctgttgct ggacgaaatc gagaaagcgc atccggacgt gttcaatatt ctgttgcagg
    45541 tgatggacaa cggtacgctg accgataaca acggacgcaa agcggacttc cgtaacgtgg
    45601 tgctggtgat gaccaccaac gccggggtac gtgaaactga gcgtaaatcc attggtctta
    45661 tccaccagga taacagcacc gatgcgatgg aagagatcaa gaagatcttt acaccggaat
    45721 tccgtaaccg tctcgacaac attatctggt tcgatcatct gtcaactgac gtgatccatc
    45781 aggtggtgga taaattcatc gtcgagttgc aggttcagct ggatcagaaa ggtgtttctc
    45841 tggaagtgag ccaggaagcg cgtaactggc tggccgagaa aggttacgac cgggcaatgg
    45901 gcgcacgtcc gatggcgcgt gtcatccagg acaacctgaa aaaaccgctc gccaacgaac
    45961 tgctgtttgg ttcgctggtg gacggcggtc aggtcaccgt cgcgctggat aaagagaaaa
    46021 atgagctgac ttacggattc cagagtgcac aaaagcacaa ggcggaagca gcgcattaat
    46081 ctgattgtta ggtaggttgg tcaagtccgt aatctcgaaa gaggttacgg actttttgtt
    46141 tatggggtgg aggaggttca gacccttttt ttaatgatga tggtaagttg ttgataatta
    46201 gtgctgcggg aaggtaagga caaaaaaggg tgctgcagga gaatgggatg gttttgcttt
    46261 atttacaacg ggctaaacgt gtagtatttg agttcactgc cgtacaggca gcttagaaat
    46321 tcacaggtaa catactccac ccgcccacca tgttcactgc cgtacagaca gataaaatgc
    46381 gaaaaaaaag ctcgcacttt cgtacgagct cttctttaaa tatggcggtg agggggggat
    46441 tcgaaccccc gatacgttgc cgtatacaca ctttccaggc gtgctccttc agccactcgg
    46501 acacctcacc aaattgtttt gctaccaaac ctcatgggtg gcaacggggc gctactatag
    46561 ggagttggag taaaacggtc aagaagaatt ttaatgataa ttattgtttg ctcatactgt
    46621 aaacaagttg tgcagtatat ctacatcgag acaagttacg gacttatact tccaaagtac
    46681 ttcatacata tcacaaaata aaaaggccgg ttaaaccgac cttttactcg ttctttctct
    46741 tcgcccatca ggcggtaaaa caatcagcga ctacggaaga caatgcggcc tttgctcagg
    46801 tcgtacgggg tcagttcaac agtcactttg tcgcccgtca ggatgcggat gtagtttttg
    46861 cgcattttac cggagatgtg tgcagtaacc acgtgaccgt tttctaactc tacgcggaac
    46921 atggtattag gcaacgtttc aagaacggta ccttgcattt caatattgtc ttctttggcc
    46981 atctaatcct ctggggtatc actaccgtaa tttgaaccgg caagataatg ccgaagttct
    47041 gtaaataagt aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac attactccga
    47101 aaacacacgg ctaagccgca ccaaaagcgc aacgtataag ggagcggtga gataaacgat
    47161 gggcgttacc tgacgcgaaa aattccttat cggcagcggg gtaatgagcg taaccaactc
    47221 tgcgaccgca attataacac tctggggaga aatgtgccga aaacattcat tcttgtggtg
    47281 aaaacaagca tcgtggtacc cagaaattat tcggcaatcg tccgaggcgc atttgattga
    47341 gataattaag gtaatcccgg cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt
    47401 taaggacctg gcagtcgata agcttaccgc catgaccgat aaactcctca cagaatacca
    47461 gaagcgccgt tttagacgca ttttccatcc ggctgaacat ggactcgcca caaaatagcg
    47521 ttccctgggc cacgccgtac ataccgccga caagctcatc ttcacgccag acttcaatgg
    47581 agtgggcatg gccgagttcg tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc
    47641 aggttccttc ttcgcgatcg ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca
    47701 tcgtgacacg atagggcgag cgtttatgaa atcgcttcat actacggctg atatgcagtg
    47761 attctggcca tagcaccgcg cggggatcgg gcgaccacca gaggatgggg tcgcctggag
    47821 aaaaccacgg aaaaatacca cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc
    47881 ccccaagtgc cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta
    47941 ttgaatggcg agaaagctga accaggcgca tgaccgcaaa actccacgca agtcggatcg
    48001 ttcaataata gcttacaaac cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag
    48061 ctctgcgtga gtaccttgct caataatttg cccgttgtcc atcactatta tttgttggaa
    48121 acgagagagt ccgcgaagtc gatgggtgac cattaacacc gttttctcac gcatcatttc
    48181 tgcaagcaat tcaaggatct ggctttcggt tgtggcatct aagccttcgg taggttcatc
    48241 cagcaacacc agtggcgcat catgtaacag cgcacgggcg atagccagac ggcgcagttc
    48301 accaccggag agctggcgtc cgccttcacc taaccaactg ttgagacctg catcctcgag
    48361 cagcttctcc agaccaacgc gacgcaagct ctccgccaga gcctcatcac tactgccagg
    48421 cgaggcgagt aacagattat cacgcagcgt ggcgctaaac agatgcactc gctgaggaac
    48481 aacgctgatg gtctgtcgta gagccgcttc attcaggctg gctatggggc tatcgttaag
    48541 caaaatctcg ccctgttgcg ggtcccaagc gcgggtcagc agttgtaaca gtgttgattt
    48601 gccgcatccg gttcgcccga gaatcgcaat atgttccccg gcgtttacct gaagagaaat
    48661 ccctttaagt gcctgttggg attgctccgg ataagtgaac tgaacatccc gtaatgtcag
    48721 cgaaacgcga tcggcaacac gagtttgggt atcagggaag gtgacttccg gtttttgatc
    48781 cgttaaatca gtgatccgaa cggcagaggc aatgacttgc cccagatgct gaaatgcacc
    48841 cgttactggt gccagtgctt caaacgcggc taacgcgcag aagacaaaca gggcaattaa
    48901 cgcgccgggt tgagcattgc cgccaacgcc gccagacgcc atccacagca tcaggatcac
    48961 cgctaacgcg ccaatgagca gcattatcgc ttgcgacaat gcggtcagtt cagattgacg
    49021 gcgttgcgct tccagccatt gaatttctgt attctctagt tgcgtgcgat aacgatcgct
    49081 ggcaccaaaa atggtcagct cagcttgccc ttgcagccag gccgtcagtt gttggcgata
    49141 ctgtccgcga agatgagtca gattttgccc ggtgcttttt cccgcacgat aaaacagcgg
    49201 tggcatcagg aaaagcgtca gtaacataat gccgcccagc gtaaaggcga gggtgaaatc
    49261 aaggaaactt aacccgattg tcaccatcat aatcaccaca aaagcgccca ccagcggcga
    49321 gataacgcgc aggtaaagat gatcgagcgt atcaacatcc gccaccacgc gattgagcaa
    49381 ttcgccctga cgatagcgcg ccagtccggc aggggagagg ggcaacaatt tgctgaaggt
    49441 gtaaatgcgc agatgctgca acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa
    49501 ataacgcccg gcagtacggg tgattgcggc accacgcacg cccgcagcgg gtagcatata
    49561 gttgaagctg tacagtccgg caacccccgc aaccgctgag gccgagagga accagccgga
    49621 aagtgtcaac agtccgatac tggcgagcag cgtcacaatt gccagcacaa taccaagact
    49681 taacatccat ttatgacgtt tatacagtgc tagatagggt agcaaagcgc gcatttaaat
    49741 ctcctcctga cgatgggcca gtaatgtggc gaatgggcca ccagccacac ttaattccgc
    49801 gtaacgtcct tgctcaataa tccggccatc ctgcataacc caaatgacat cccagtcagc
    49861 aagatcttct aactggtggg tgaccattaa cgttgtctgg cgcagagagg cggcattcag
    49921 cgcctccatt acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt catccaacag
    49981 taatagcgaa cagggattta gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac
    50041 ggaaaggcgg gcagcctggt cgccaacagg cgtatcaacg ccttgtggca ggagcggtag
    50101 aaactcgctg acccaggcgt tatccagcgc tgcttgtaat tcatgttcgc tggcatcagg
    50161 tcgcgccagt agtacgttat cccgtaatgt tgctgccggt aattgtggat tttgcccaac
    50221 ccaggagaga tgtttacgcc atgattctgg tgataaatcg cgtaattcta tcccgttgat
    50281 tcgtagcgat ccctgatatg agagaaaacc agaaagcgcg ttcaacagtg aacttttacc
    50341 tgaaccgctg cgaccaacca acacggcacg ttggcctgct ggcaaagtaa agttcagcgg
    50401 tccggccagc gttttacctt ccggcgacgt gataaacaga tcctcggcct caatggtcac
    50461 cggatcggtc gatgctaatt ccacctcgcc acactgcgga tgggcgagcg gggtttccat
    50521 aaacgttttc agactgtcag ctgcaccaac agcctgggct ttagcatgat aaaacgtacc
    50581 gagatcgcgt aatggctgga aaaactctgg cgcaaggatc agggccagaa aacccgcagc
    50641 cagcgtcacg ccagtatcgt agtgaccaaa atccagctcg ccaagatagg aaaaaccaaa
    50701 gtagaccgcc accagagcaa ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa
    50761 aaatgccagc cgtagcactt ccattgtccg ttggcggaaa tcttccgaag cagaacgaat
    50821 actttcaatt tcagcttcac cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg
    50881 atcgaggaaa tgcccactta agcgagcaag agcgagaaag ttacgtcggt tagcatcggc
    50941 agcccccatt ccaaccagcg ccataaacaa cggaattaac ggtgcagtgc ccagcagaat
    51001 gagcgccgca gcccagtttg aggggaagat tgccaccaca atcagcaacg gcaccgacac
    51061 tgccagcgcc atttgcggca ggtagcgtgc atagtaatca tgcatatcgt caatttgctc
    51121 gagtaccagc gtcgcccagc tccccgcagg tttaccctga atccacgctg gccctgcttg
    51181 ttgcagacgg tcgagaacct gccggcggat ggcaaagcgg atatgctgcc cggcgtgata
    51241 acccacccgt tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca gaaccagtaa
    51301 cgtaaaggga agcagcaggg cttcacgggg aatattctcc ataatcatat gttgcagaat
    51361 tcgcgccatg aaccaggcct gggcaatgat caatatgccg ctcacaaagc ccagcagacg
    51421 agaaatattc agccaacgtt gggagatgac gctttgctgt tttaaccagc gggttaactc
    51481 tttttgacga gatttattca ttgcacgctt agcaggtgag ttatcagaat tatttgcaga
    51541 gcaatgttac aacggggaaa aaataaaggc gacccatagt cgcaaggtgt cgccttcttt
    51601 acttttgtta ctgatttgta aaattatttt gcgtcagcta aaccatcgag gtagcgttcc
    51661 gcatcaagtg ctgccatgca gcctgtaccg gccgaagtaa tggcctgacg ataaatatga
    51721 tccatcacgt cacccgcggc aaagacgcca ggaatgctgg tctgggtggc attaccatga
    51781 atacccgact gtactttgat gtagccgttt tccagttcca gctgcccttc gaaaatggca
    51841 gtattcgggc tgtgaccgat agcaacaaac agaccggcaa cgtcgagtga ttcgatgtta
    51901 tcgctgtttt gcgtatcgcg cagacgaacg ccagtgacac ccatttgatc gccggtcact
    51961 tcttccagcg tccggttggt gtgcagaatg atgttgccgt tctccacttt atccatcaga
    52021 cgcttaatga ggattttttc cgcgcggaaa ccgtcacggc ggtgaatcag atgtacttcc
    52081 gaagcgatgt tagacagata caacgcctct tcaaccgcgg tattgccgcc gccgatgacc
    52141 gcaactttct ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg
    52201 cctttaaagg cttcttcaga gggcaggccg agatagcgtg cagaagctcc ggtggcaata
    52261 atcagcgcgt cgcaagtgta ttcgccgtta tcgccattca gacggaacgg acggttttgc
    52321 agatccacct tgttgatatg atcaaaaatg atttcagttt caaatttggt ggcatgttcg
    52381 tgcatgcgct ccattaataa cggaccggtc agatcgtttg gatcgccagg ccagttttcc
    52441 acttctgtgg tggtggtcag ttggccgcct ttttccatgc cggtaatcag cacaggttgc
    52501 aggttggcgc gcgccgcata gacagcagcg gtgtatcccg ccgggcctga acccaggata
    52561 agcagtttac tgtgtttggt cgtgcccatg agatccccat agttgttggc agacaatggg
    52621 caggattgta gggaatttac tgacgtaaaa aaagagtatg acgattttgt taacaatttg
    52681 tgcaatcggc agcatcgata agcaggtcaa attctcccgt cattatcacc tctgctactt
    52741 aaatttcccg ctttataagc tgattaaatg atgaataaac gcccctgtta atgaatatct
    52801 ggcatgttgt actaaaaatc gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac
    52861 attcgaggaa gaaaaaaaac agtattctta tatgcgcata accatgcatg taaataccat
    52921 gtttaccgtg ctagtgaaat ctacgtatgg cgtggacaga cgccattcgt gatgtcgata
    52981 gctgccacaa ggcaacggtc ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc
    53041 atgatttcgg tctatcgtga cgggtagcga ctctgaacag tgatgtttca gggtcagaca
    53101 ggagtaggga aggaatacag agagacaata ataatggtag atagcaagaa gcgccctggc
    53161 aaagatctcg accgtatcga tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt
    53221 tctaacgtcg agctttctaa acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg
    53281 cgtcggctgg aaagacaagg gtttattcag ggctatacgg cgctgcttaa cccccattat
    53341 ctggatgcat cacttctggt attcgttgag attactctga atcgtggcgc accggatgtg
    53401 tttgaacaat tcaataccgc tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta
    53461 tctggtgatt tcgactacct gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag
    53521 ttgctggggg aaaccctgct gcgtctgcct ggcgtcaatg acacacggac atacgttgtt
    53581 atggaagaag tcaagcagag taatcgtctg gttattaaga cgcgctaaca cggaacaggt
    53641 gcaaaatcgg cgtattttga ttacactcct gttaatccat acagcaacag tactggggta
    53701 acctggtact gttgtccgtt ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt
    53761 tcttgagcca ggaatacact gaagacaaag aagtcacatt gacaaagtta agtagcggcc
    53821 gtcgccttct ggaagcgttg ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg
    53881 ccttactaag ctttaaccct tcggacccca gctggtcgca aacggcctgg catgaaccta
    53941 tccataattt aggtgggatg cccggtgcgt ggctggcaga tacgctgttc tttatttttg
    54001 gcgtgatggc ttacaccatt cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc
    54061 atcagtccag tgacgaatac attgattatt ttgccgtttc gttacgcatc attggcgttt
    54121 tggcgctcat ccttacctcc tgtggtctgg cggcaatcaa cgctgacgat atctggtatt
    54181 ttgcctccgg tggcgtcatt ggcagcttac taagcactac gttacaacca ctgctacaca
    54241 gtagcggggg aactattgcg ctgctctgcg tttgggctgc gggcctgacg ctgttcaccg
    54301 gttggtcatg ggtgaccatt gctgaaaaac tcggcggctg gattttaaac attctcacct
    54361 tcgccagtaa tcgtacccgt cgcgatgata cctgggtcga tgaagatgaa tatgaagatg
    54421 acgaagagta tgaagatgaa aaccacggca aacagcatga atcacgccgt gcccgtattc
    54481 ttcgcggcgc gctagcgcgt cgtaaacggt tggcggaaaa attcattaat ccgatggggc
    54541 ggcaaacaga cgctgcgttg ttctccggta agcggatgga tgatgaagaa gagattatct
    54601 acactgcacg cggtgtggct gccgacccgg acgacgtcct attttcgggc aatcgtgcaa
    54661 cgcagccaga atatgacgaa tacgatccat tattaaacgg tgcgccaatt accgaacctg
    54721 ttgctgtagc agctgctgct accacggcga cacaaagctg ggctgcgccg gttgaacctg
    54781 tgacccagac gccgcctgtt acttctgttg atgttccacc ttcgcaacct acagtagcct
    54841 ggcagcctgt accgggtcca caaacgggag agccggttat tgctcctgca ccggaaggtt
    54901 acccacagca gccacaatat gcgcagcctg cagtgcaata taatgagccg ctgcaacaac
    54961 ctgtacagcc acagcagccg tattatgcgc ctgcagctga acaacctgtg caacaacctt
    55021 actacgctcc tgcggcggaa caacctgttc agcaaccgta ttacgcaact gcgccggaac
    55081 agcctgcgca acagccgtat tatgcccctg cgccagaaca accggtggca ggtaacgcct
    55141 ggcaagccga agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa
    55201 cttatcagca gccagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc
    55261 agcctgttgt ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt
    55321 actattttga agaagtggaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt
    55381 atcaaccgat tccagaaccg gttaaagaac cagaaccgat caaatcttcg ttgaaagcgc
    55441 cttctgctgc agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg
    55501 gcgtgaaaaa agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca
    55561 gtctggcaaa tagcggtgga ccgcgtccgc aagtcaaaga gggaattggc ccgcagttgc
    55621 cacgaccgaa acgtattcgc gtgccaactc gtcgtgaact ggcgtcttac ggtattaagc
    55681 tgccctcaca gcgtgcggcg gaagagaaag cccgtgaagc ccggcgcaat cagtatgatt
    55741 ctggcgatca gtataacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc
    55801 agttcgccca gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa
    55861 acgcagaaga tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc
    55921 aacaacaacg ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt
    55981 ttgaattttc gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc
    56041 caattgttga acctgtacag cagccgcaac aactggttgc accgcagcag cagtatcagc
    56101 agccacaaca gccggttgcg ccacagcagc aatatcagca gccgcaacag ccggttgcgc
    56161 cgcagccgca gtatcagcag ccacaacagc aggttgcgcc gcagccacaa tatcagcagc
    56221 cgcaacaacc ggttgcaccg cagcagcagg ataccctgct tcatccgctg ttgatgcgta
    56281 atggcgacag ccgtccgttg cataaaccga cgacgccgct gccttctctg gatttgctga
    56341 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggctcgcc
    56401 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgtcaat tactctccgg
    56461 ggccggttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    56521 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    56581 ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt
    56641 atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    56701 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    56761 tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga
    56821 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    56881 tggagctttc ggtttatgaa ggcattccgc atctgttaac ggaagtcgtt actgatatga
    56941 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgt tataaactga
    57001 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    57061 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    57121 atccggtgct gaaaaaagaa ccatacattg tggtgttggt tgacgaattt gccgacctga
    57181 tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    57241 ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    57301 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    57361 gtaccattct tgatcaggct ggcgcggaat cactgctggg gatgggggat atgctctact
    57421 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    57481 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    57541 cttccgacag tgaaagcgaa ggtggcgcgg gcggtttcga tggcgctgaa gaactggatc
    57601 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    57661 tacagcgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    57721 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    57781 cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    57841 caactgaaaa ttcagtattt tcttttttcc tcaagctgat tattagcctg gaatagagag
    57901 tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgcc
    57961 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    58021 gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg
    58081 actgacggta gcggtgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    58141 aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa
    58201 acactgtggt tctataaccc gttcgttgag caagctacgg caacttggct gaaagatgcc
    58261 accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    58321 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    58381 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    58441 caggacgatc agcgcagcag ttatcaactg aaatcccagc aaaatggggc tgtggatgca
    58501 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    58561 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    58621 gcgtatgcgg ccagaaaatt tagcacagta tatcggccag caacatttgc tggctgcggg
    58681 gaagccgttg ccgcgcgcta tcgaagccgg gcatctgcat tctatgatcc tctgggggcc
    58741 accgggtacc ggcaaaacaa ctctcgctga agtgattgcc cgctatgcga acgctgatgt
    58801 ggaacgtatt tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc
    58861 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    58921 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    58981 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg
    59041 tgtctatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    59101 ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg aaacacgacg
    59161 cgccattgct gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    59221 ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg aattactgac
    59281 cgaaatcgca ggtgaacgta gtgcccgctt tgataacaaa ggtgatcgct tttacgatct
    59341 catttccgca ctgcataaat cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    59401 tgcgcgaatt attactgccg gtggcgatcc gttatatgtt gcgcgtcgct gtctggcgat
    59461 tgcgtctgaa gatgtgggta atgctgatcc acgagcgatg caggtggcaa ttgcagcctg
    59521 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    59581 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ctgcgctggc
    59641 ggatgctcgc gaacgtccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    59701 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    59761 ttatgctgcc ggtgaggttt acttcccgcg ggaaatagca caaacacgct attatttccc
    59821 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    59881 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    59941 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    60001 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    60061 ccggggcttt aagctggatg tagataagct gggcgctctc gaagagcgtc gtaaagtatt
    60121 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    60181 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    60241 agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc
    60301 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    60361 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    60421 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    60481 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    60541 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    60601 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    60661 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    60721 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    60781 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    60841 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga
    60901 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agctgctggg
    60961 cctgccgtac cgtaaaatca tcctttgtac cggcgacatg ggctttggcg cttgcaaaac
    61021 ctatgacctg gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc
    61081 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa
    61141 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    61201 tgcagtaatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    61261 tccgtatatg aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag
    61321 cgcctgcggg cgcttttttt gtctcccttt gataccgaac aataattact cctcacttac
    61381 acgtaatact actttcgagt gaaaatctac ctatctcttt gattttcaaa ttattctatg
    61441 tatacaagcc tatatagcga gctgctatag aaataattac acaatacggt ttgttactgg
    61501 aatcaatcgt gagcaagctt gagtgagcca ttatgaaaac gaaaatccct gatgcggtat
    61561 tggctgctga ggtgagtcgc cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa
    61621 tggccagcag cgcattaaca ttacctttta gtcggattgc gcacgctgtc gatagcgcca
    61681 ttccaacaaa atcagacgaa aaggttatct ggagcgcctg tacagttaac tgtggtagtc
    61741 gctgcccgct acgtatgcac gtcgtggacg gtgaaatcaa atatgtcgaa acggacaata
    61801 ccggcgatga caattacgac ggcctgcact aggttcgcgc ctgcctgcgt gggcgttcca
    61861 tgcgtcgccg tgtctacaat ccggaccgcc tgaaatatcc gatgaaacga gtcggggcgc
    61921 gcggtgaagg caaattcgag cgcattagct gggaagaagc ctacgacatc atcgcgacca
    61981 atatgcagcg cctgatcaaa gagtacggca acgagtctat ctatctgaac tatggcaccg
    62041 gtacgctggg cggcaccatg acccgctcct ggccgccggg aaataccctg gtcgcgcggc
    62101 tgatgaactg ctgcggcggc tatctgaacc attacggcga ctactcctcc gcgcaaattg
    62161 cggaaggttt gaactatacc tacggcggct gggcagatgg caacagcccg tcggatatcg
    62221 aaaacagtaa gctggtagtg ctgtttggta ataaccctgg cgaaacgcga atgagtggca
    62281 gtggggtgac ttactatctt gaacaggcac gccagaaatc taatgcccgc atgatcatca
    62341 tcgatccgcg ctataccgac accggtgccg ggcgcgaaga tgagtggatc cctattcgtc
    62401 cgggaacaga tgccgcactg gttaacggtc tggcgtacgt catgatcact gaaaacctgg
    62461 tggatcaggc attcctcgat aaatattgcg ttggctacga tgagaaaacc ctgccagcca
    62521 gtgcgccgaa aaatggccac tataaagctt atattctggg tgaagggcca gatggcgtgg
    62581 ctaaaacacc ggaatgggcc tcacaaatca ccggtgttcc ggcagacaaa atcatcaaac
    62641 tggctcgtga aatcggcagt accaaaccgg cgtttatcag ccagggatgg ggcccgcagc
    62701 gtcacgctaa cggtgaaatc gcaacccgtg ctatctcgat gctggcgatt ctgaccggta
    62761 acgttggtat taacggaggc aacagcggcg cgcgtgaagg ttcatacagc ttaccgtttg
    62821 tccgtatgcc gaccttggaa aacccgatcc agaccagcat ttcgatgttt atgtggaccg
    62881 atgccattga acgtggcccg gaaatgacgg cgctgcgtga tggtgtacgc gggaaagata
    62941 agctggatgt gccgatcaaa atgatctgga actatgccgg taactgcctg attaaccagc
    63001 attctgaaat caaccgtacc catgaaatcc ttcaggatga taagaagtgc gagctgattg
    63061 tggttatcga ctgccacatg acctcatcgg cgaaatatgc tgacatcctg ctgcctgact
    63121 gcaccgcttc cgaacagatg gactttgcgc tggatgcatc ctgcgggaat atgtcttacg
    63181 tgattttcaa cgatcaggtg attaaaccgc gctttgaatg taagaccatc tatgaaatga
    63241 ccagcgaact ggcaaaacgt cttggcgttg agcaacagtt tactgaaggc cgtacacagg
    63301 aagagtggat gcggcatctg tatgcccagt cgcgggaagc gattcctgaa ctaccaacgt
    63361 ttgaagagtt ccgcaagcag gggatcttta aaaagcgcga cccacaaggg caccacgttg
    63421 cttataaagc tttccgtgaa gatcctcagg caaattcact gaccacgcca tcgggcaaaa
    63481 ttgagattta ttcgcaggcg ctggctgaca ttgccgctac ctgggaattg ccagaaggag
    63541 atgtgatcga tccactgccg atctacacgc cgggctttga aagttatcag gatccgttga
    63601 acaaacagta tccgctgcag cttacgggtt tccactataa atctcgcgtt cactcaactt
    63661 acggcgacgt tgatgtgctg aaagcagctt gccgtcagga aatgtggatc aacccgcttg
    63721 atgcgcaaaa acgcggtatc aacaacggcg ataaagtcag gatctttaac gatcttggtg
    63781 aggttcatat tgaggcgaaa gtgacgccac gaatgatgcc gggtgtggtc gcactgggtg
    63841 aaggtgcctg gtatgacccg gatacaaaac gtgtcgataa gggcggttgt atcaacgtac
    63901 tgaccactca acgtccgtct cctctcgcta aggggaatcc gtcacataca aaccttgttc
    63961 aggttgaaaa ggtgtaagga gtaaccgatg acaacccagt atggattttt tattgattcc
    64021 agccgttgca c
//
LOCUS       DAJVTY010000006        58357 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVTY010000006 DAJVTY010000000
VERSION     DAJVTY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58357
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.006"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..567)
                     /locus_tag="OQQ66_000374"
     CDS             complement(<1..567)
                     /locus_tag="OQQ66_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1903422.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKL"
     gene            complement(693..3830)
                     /gene="sbcC"
                     /locus_tag="OQQ66_000375"
     CDS             complement(693..3830)
                     /gene="sbcC"
                     /locus_tag="OQQ66_000375"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1903423.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALSDLVSHKT
                     RIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVKKI
                     NGLGYSKLESTFAVK"
     gene            complement(3827..5029)
                     /gene="sbcD"
                     /locus_tag="OQQ66_000376"
     CDS             complement(3827..5029)
                     /gene="sbcD"
                     /locus_tag="OQQ66_000376"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1903424.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            5219..5908
                     /gene="phoB"
                     /locus_tag="OQQ66_000377"
     CDS             5219..5908
                     /gene="phoB"
                     /locus_tag="OQQ66_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1903425.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            5966..7261
                     /gene="phoR"
                     /locus_tag="OQQ66_000378"
     CDS             5966..7261
                     /gene="phoR"
                     /locus_tag="OQQ66_000378"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1903426.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            7668..8987
                     /gene="brnQ"
                     /locus_tag="OQQ66_000379"
     CDS             7668..8987
                     /gene="brnQ"
                     /locus_tag="OQQ66_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1903427.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9063..10436
                     /gene="proY"
                     /locus_tag="OQQ66_000380"
     CDS             9063..10436
                     /gene="proY"
                     /locus_tag="OQQ66_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1903428.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            10592..12314
                     /gene="malZ"
                     /locus_tag="OQQ66_000381"
                     /pseudo
     CDS             10592..12314
                     /gene="malZ"
                     /locus_tag="OQQ66_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(12319..12900)
                     /gene="acpH"
                     /locus_tag="OQQ66_000382"
     CDS             complement(12319..12900)
                     /gene="acpH"
                     /locus_tag="OQQ66_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS1903429.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     YRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            12993..14063
                     /gene="queA"
                     /locus_tag="OQQ66_000383"
     CDS             12993..14063
                     /gene="queA"
                     /locus_tag="OQQ66_000383"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1903430.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            14119..15246
                     /gene="tgt"
                     /locus_tag="OQQ66_000384"
     CDS             14119..15246
                     /gene="tgt"
                     /locus_tag="OQQ66_000384"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1903431.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            15269..15601
                     /gene="yajC"
                     /locus_tag="OQQ66_000385"
     CDS             15269..15601
                     /gene="yajC"
                     /locus_tag="OQQ66_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1903432.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            15629..17476
                     /gene="secD"
                     /locus_tag="OQQ66_000386"
     CDS             15629..17476
                     /gene="secD"
                     /locus_tag="OQQ66_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1903433.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            17487..18458
                     /gene="secF"
                     /locus_tag="OQQ66_000387"
     CDS             17487..18458
                     /gene="secF"
                     /locus_tag="OQQ66_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1903434.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            18587..18934
                     /locus_tag="OQQ66_000388"
     CDS             18587..18934
                     /locus_tag="OQQ66_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1903435.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(19111..19995)
                     /gene="tsx"
                     /locus_tag="OQQ66_000389"
     CDS             complement(19111..19995)
                     /gene="tsx"
                     /locus_tag="OQQ66_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1903436.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20294..20833)
                     /locus_tag="OQQ66_000390"
     CDS             complement(20294..20833)
                     /locus_tag="OQQ66_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1903437.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTPLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            20984..21433
                     /gene="nrdR"
                     /locus_tag="OQQ66_000391"
     CDS             20984..21433
                     /gene="nrdR"
                     /locus_tag="OQQ66_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1903438.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            21437..22540
                     /gene="ribD"
                     /locus_tag="OQQ66_000392"
     CDS             21437..22540
                     /gene="ribD"
                     /locus_tag="OQQ66_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1903439.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            22629..23099
                     /gene="ribE"
                     /locus_tag="OQQ66_000393"
     CDS             22629..23099
                     /gene="ribE"
                     /locus_tag="OQQ66_000393"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1903440.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            23119..23538
                     /gene="nusB"
                     /locus_tag="OQQ66_000394"
     CDS             23119..23538
                     /gene="nusB"
                     /locus_tag="OQQ66_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1903441.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            23616..24593
                     /gene="thiL"
                     /locus_tag="OQQ66_000395"
     CDS             23616..24593
                     /gene="thiL"
                     /locus_tag="OQQ66_000395"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1903442.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            24571..25089
                     /gene="pgpA"
                     /locus_tag="OQQ66_000396"
     CDS             24571..25089
                     /gene="pgpA"
                     /locus_tag="OQQ66_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1903443.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(25143..26117)
                     /gene="yajO"
                     /locus_tag="OQQ66_000397"
     CDS             complement(25143..26117)
                     /gene="yajO"
                     /locus_tag="OQQ66_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1903444.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLFYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(26172..28034)
                     /gene="dxs"
                     /locus_tag="OQQ66_000398"
     CDS             complement(26172..28034)
                     /gene="dxs"
                     /locus_tag="OQQ66_000398"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1903445.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(28059..28958)
                     /gene="ispA"
                     /locus_tag="OQQ66_000399"
     CDS             complement(28059..28958)
                     /gene="ispA"
                     /locus_tag="OQQ66_000399"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1903446.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(28958..29200)
                     /gene="xseB"
                     /locus_tag="OQQ66_000400"
     CDS             complement(28958..29200)
                     /gene="xseB"
                     /locus_tag="OQQ66_000400"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1903447.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            29406..30854
                     /gene="thiI"
                     /locus_tag="OQQ66_000401"
     CDS             29406..30854
                     /gene="thiI"
                     /locus_tag="OQQ66_000401"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1903448.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(30908..31498)
                     /gene="yajL"
                     /locus_tag="OQQ66_000402"
     CDS             complement(30908..31498)
                     /gene="yajL"
                     /locus_tag="OQQ66_000402"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1903449.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(31461..32372)
                     /gene="panE"
                     /locus_tag="OQQ66_000403"
     CDS             complement(31461..32372)
                     /gene="panE"
                     /locus_tag="OQQ66_000403"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1903450.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            32540..33031
                     /gene="yajQ"
                     /locus_tag="OQQ66_000404"
     CDS             32540..33031
                     /gene="yajQ"
                     /locus_tag="OQQ66_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1903451.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(33159..34523)
                     /locus_tag="OQQ66_000405"
     CDS             complement(33159..34523)
                     /locus_tag="OQQ66_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1903452.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(34672..35562)
                     /gene="cyoE"
                     /locus_tag="OQQ66_000406"
     CDS             complement(34672..35562)
                     /gene="cyoE"
                     /locus_tag="OQQ66_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1903453.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(35574..35903)
                     /locus_tag="OQQ66_000407"
     CDS             complement(35574..35903)
                     /locus_tag="OQQ66_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1903454.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(35903..36517)
                     /locus_tag="OQQ66_000408"
     CDS             complement(35903..36517)
                     /locus_tag="OQQ66_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1903455.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(36507..38498)
                     /gene="cyoB"
                     /locus_tag="OQQ66_000409"
     CDS             complement(36507..38498)
                     /gene="cyoB"
                     /locus_tag="OQQ66_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS1903456.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(38520..39467)
                     /gene="cyoA"
                     /locus_tag="OQQ66_000410"
     CDS             complement(38520..39467)
                     /gene="cyoA"
                     /locus_tag="OQQ66_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS1903457.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(39928..41403)
                     /gene="ampG"
                     /locus_tag="OQQ66_000411"
     CDS             complement(39928..41403)
                     /gene="ampG"
                     /locus_tag="OQQ66_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1903458.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(41447..42025)
                     /locus_tag="OQQ66_000412"
     CDS             complement(41447..42025)
                     /locus_tag="OQQ66_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1903459.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            42330..42647
                     /gene="bolA"
                     /locus_tag="OQQ66_000413"
     CDS             42330..42647
                     /gene="bolA"
                     /locus_tag="OQQ66_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1903460.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            42991..44289
                     /gene="tig"
                     /locus_tag="OQQ66_000414"
     CDS             42991..44289
                     /gene="tig"
                     /locus_tag="OQQ66_000414"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1903461.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            44534..45157
                     /gene="clpP"
                     /locus_tag="OQQ66_000415"
     CDS             44534..45157
                     /gene="clpP"
                     /locus_tag="OQQ66_000415"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1903462.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            45283..46557
                     /gene="clpX"
                     /locus_tag="OQQ66_000416"
     CDS             45283..46557
                     /gene="clpX"
                     /locus_tag="OQQ66_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1903463.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            46745..49099
                     /gene="lon"
                     /locus_tag="OQQ66_000417"
     CDS             46745..49099
                     /gene="lon"
                     /locus_tag="OQQ66_000417"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1903464.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            49308..49580
                     /gene="hupB"
                     /locus_tag="OQQ66_000418"
     CDS             49308..49580
                     /gene="hupB"
                     /locus_tag="OQQ66_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1903465.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            49772..51643
                     /gene="ppiD"
                     /locus_tag="OQQ66_000419"
     CDS             49772..51643
                     /gene="ppiD"
                     /locus_tag="OQQ66_000419"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1903466.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            51794..52165
                     /locus_tag="OQQ66_000420"
     CDS             51794..52165
                     /locus_tag="OQQ66_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1903467.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            52259..52657
                     /gene="fadM"
                     /locus_tag="OQQ66_000421"
     CDS             52259..52657
                     /gene="fadM"
                     /locus_tag="OQQ66_000421"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1903468.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(52709..53404)
                     /gene="queC"
                     /locus_tag="OQQ66_000422"
     CDS             complement(52709..53404)
                     /gene="queC"
                     /locus_tag="OQQ66_000422"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1903469.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(53469..55169)
                     /locus_tag="OQQ66_000423"
     CDS             complement(53469..55169)
                     /locus_tag="OQQ66_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS1903470.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            55269..56051
                     /gene="cof"
                     /locus_tag="OQQ66_000424"
     CDS             55269..56051
                     /gene="cof"
                     /locus_tag="OQQ66_000424"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1903471.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTH"
     gene            56240..56698
                     /gene="decR"
                     /locus_tag="OQQ66_000425"
     CDS             56240..56698
                     /gene="decR"
                     /locus_tag="OQQ66_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1903472.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            56728..>58357
                     /locus_tag="OQQ66_000426"
     CDS             56728..>58357
                     /locus_tag="OQQ66_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1903473.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQNLHNLRQWGQGRTVIISAHRLSALTEASENVMN"
BASE COUNT        14068 a        14604 c        15669 g        14016 t
ORIGIN      
        1 cagctttcct ttcgcctcgt cgcgaatatc cggcacccaa aaatagaccg atgccatcac
       61 cgcaatatta aaaacagcga tcaataaaaa ggtgtaacgc cagctaaatt cctgacttaa
      121 atacgttccc agcggaatgc ccagcaaatt ggcgactgtc atcccggaaa ccatccccgc
      181 cacggcggcg gtgacttttc cgggtttgat aatttttgat aacacgatcg cgccgacgcc
      241 aaaaaatgcg ccatgcggaa agccggatac cagccgacca atggcgagca tcaggtaaga
      301 cgaagagagc gtgaacatgg cgttgccaat gacgcacaac gccaccagaa acaacaagat
      361 atgtttgagt gagtagcggc tggaaaagag tgcgatgatt ggcgcaccga ccaccactcc
      421 cagtgcataa tacgagatca tatgcccggc agcaggaatc gaaattccta cgttatgcgc
      481 cagctccgtg agcacgccca taatgccaaa ttcggccatc cccaaaccaa acgtgcccag
      541 agccaaagat aaaatgactt ttttcatacc accatcacaa ccaccagaac ggctaccaat
      601 caccgtagcc aataaagtga tagcttgcag gataacgacc agcaaggcca agagataatt
      661 cgcccctctg tattcattat cctgctgaat agttatttca ctgcaaacgt actttccagt
      721 ttgctgtagc ccaggccgtt gatctttttc actttgatct gcaccggaat acgctctttc
      781 atcgcttcta cgtggctaat cacaccgatg gttttgccac tggcgttcag ggcatccagc
      841 gcatcaaggg cggtatccag cgtttcgcta tccagcgtgc caaaaccttc atcaaggaac
      901 agcgagtcaa tacgtgtttt atggctgacc agatccgaaa gcgccagcgc cagactaacg
      961 aggaaacttt cgccgccgga aagggtacgg gtatcgcgta ccgcatctgc ctgccaggta
     1021 tcaacaacct cgacttccag cgcctcgctg gctttgcgct gtaacagata gcgcccgtgc
     1081 agccgggtaa gttgctgatt agcgagatgg actaaattat ccagcgtcag cccctgggca
     1141 aacttgcgga atttatcgcc ctctttggaa cctattagcg aattcagata tccccagtcc
     1201 tcaacctgct gcgtcatttg agcaatttgc tgcattaagg tttgttgttg ctgacggtta
     1261 tctgcatcct gcttcagctg ctggcgaatc tcgccttgac tcgtggtgtt ttcacgcaac
     1321 ttttggtgag tttgcgctaa ctcttgctga atctgctcca ccgtcacagt gagagccaac
     1381 ccgtcaggtc ggtgttgttg atgctgtgcc agcgtttctg ctgtctgagt gaccagcgtt
     1441 tgcgcctgac ggcgctggtt ttccagattc tgcttgagct gttccagctg cgttagtgtt
     1501 tgttcatcca ttagcgccgc aaggaacgcc tgctgatcgt caaagacgct ggcctgtagc
     1561 gcggtgtcaa actgcgcctg ggctttttgc agactttgcg ccgccagaac atcctgttgc
     1621 tgtaacgtct gctgctggct gtgtaatgcg agacattgtt catgaacctg ccgccagtta
     1681 tccagcgcca cagtttcttc gctgtgcggg agatcatcac tttgcggcaa cgtttccaga
     1741 atcggcgtca gctgctgaat acggttttgc agcgtggtta attcgttctg gcgttgctgc
     1801 cagctctgcg cttcttgctg acgtgtcgcc aaccagctct cttcttcatc ttcctgtggc
     1861 aatgtcagtg cataacccgc caatgccgtt aagagctgct gctggcgttg ttcaatttgc
     1921 tgttgatact ggataatttg ctgattatgc gcggcaatct gcccttgtaa ttcatgtcgt
     1981 tggctgagta accgcagctg gcgtttgtgc tcatcttgtg catccagcca cggttgaata
     2041 tcgtcctgtg gctgcaaggt gatattgagg ctggccgtga cggcttgcca ttgttgagta
     2101 agtgcttgct catcttgtcg gaggctttgc gcttcgtttt catcgcgctg aagctgcttc
     2161 gttaatgcat ccagttgccc gcgcagcgcc gcaccttctt cgccgagctt tttaacttcg
     2221 ttttccagcg ccagtaatcg agactgatta acaccaggct ccagcgcctg atacgccttg
     2281 accgccgggt ggctggtgga accacaaagt ggacaaggct gacccgcctg taactgtgca
     2341 cgctgggctt ccagcgtttt gatgcgcgct tcctgctcgc aaatggtttt cacatcggca
     2401 agttgctgcg tcttttcttt ataacgctgg cgcatttcgt taagtgcgac attacgttgc
     2461 gtctgttcca gagatccatt ctggatagtg accattaact gcgccagacg tttttgttgg
     2521 ggaacaatct gcccatgcag cgcgaccagg cgctgacgca gtgggcgttg ctcagcatgt
     2581 tgcgccaggg cggtagcaac ttcatcggcg gttaacgtca acgtgatcgc cgcaagcgca
     2641 ttaagttttt gctcagcatg ggttaactgt tgctgccatt gccgcagatg ctcgcgatcg
     2701 ctggtttgtt gggagaactg cgcacgccaa cccgccagtt cgttgttcca ctgacggaag
     2761 cggtcgtgtt cctgtaacca ggtattcagg ctttgttgct gctgctgtaa ttctgctgac
     2821 tgcttcgccg cgtggtggcg aatgctcgcg cgaagcgcca ttgtgctctg taagcgagta
     2881 tttacttctt caatctgctg gcgagtatgc gccagcgccg cgctgtgttc tgcgatgcgt
     2941 tcccagtgcg gacgaagatt tcgtgccggt tgtgccagac tgagcgccgc cagttgaggt
     3001 tgcgcttttt cttcttcggc taacgcctgt tgcaaggcct gctgacggcg gctggcttct
     3061 tgctgcaatt cgtccagacg cgttaaccag tttagtgatt gttgttcttg ctgctgcgcg
     3121 gtaagtaact gtttttcttc gtcagtaagt acctgcaaac tcgctgtcag cgattgcact
     3181 tgttccggcg tgagcaacgc gacgccgctg gcctgcgctt gcagcttctc cagctctgtg
     3241 cgggccgatt tgtgctgctc aaaaaccatc gccgagattt gcccgtagat ttcagtgccg
     3301 gttaactcct cgagcaattc cgcgcgttct ttgggtttgg cattcaggaa ggcagcaaat
     3361 tgcccctgcg aaagcagcat cgaacgggtg aagcgcccgt aatccagccc ggttaacgtc
     3421 gctgtcagtt ccagcttatc tttcactttg tcggcgagaa ttttgccgtc ggcgcagcgc
     3481 gccagctcta cgcgtggcac ctgcaaatta ccgtcgggtt ggttacgcgc ccgattctgg
     3541 ctccagaatg cacggtacgc ttcacctttc acttcaaact ccacctccgc cagacattcg
     3601 gcggtatcgc gggtcatgag atcattttgc gattgtgaaa cgttagagag acgcggagtt
     3661 tcgtgataca gcgccagaca aatggcgtcc agcagggtgg ttttccccgc accggttggg
     3721 ccggtaatgg caaacagccc gttgctggcg aacggctcgc gggtgaaatc aatcttccat
     3781 tcgcctttta atgagttcag gtttttcagg cgcaggctga gaattttcat gcttcgtgtt
     3841 ctccggcgag ggtatgcaac gtcgtggtga aaagatgctg cagacgttgc tgctgcgatt
     3901 catccagttc ttccagtgcc agacggcgat tgaacacctc ttcgacgctg agttcgctga
     3961 gggtttcacg ctgttggctg gctaacacac gctcgcgctg ttcacgactc cgacgtacca
     4021 gcaatacttc gacaggcaat gattcggcta atgcctggat tttgcgctga atatcatgca
     4081 gatactcatc agtagtgatt tcgatatcca gccagacagg tggctcctgc gatacatcgc
     4141 gccactgttc cagctgtgcg gtaatcgacg ccagatcgcc tttcagcacc gccatgggtt
     4201 gagttaccgg tacgttcagg ttttccacgc tctctaattt gccgtttgaa aatgtcacca
     4261 gatggacata tttactctta ccgcattcat caaaactcag tggaatgggg gaaccgcaat
     4321 agcgaacatg ttccatgccg ccaataatct gtgcgcggtg aatatgcccg agcgcgatgt
     4381 agtcggctgg tggaaagttt tgtgccggaa acgcgtccag cgtgccaata taaatgtcac
     4441 gcacggcgtc acttttactg gcacccacgg tcgttaaatg tcccgtggcg atgatgggca
     4501 gaggctgatc gccgcgcagt ttgcaggcat cggcatagtg ttgttggtaa taatcggtaa
     4561 tcgctgccag taaatgctgc tgtttttcaa taccgttaag ccccgcctgg ctggaaataa
     4621 tgtcacgcgg gcgtaaaaac ggaatggggc acagcactgc gcctggcgtc ccgtcgcgac
     4681 gaggcaagat ttgcggcgca tgtccggcgc tggcgaccac ggttgtattg aggaacgcca
     4741 tgatatcgcg cgattcattc agcgtggcga ccgagtcatg gtttcctgcc agtaccacca
     4801 gatgacagcc agtttgctgt aaattgacaa caaaacggtt gtataacgtg cgggcgtaac
     4861 tgggcggcga gccggtatcg aaaacatcac cagcaacaat aatcgcatcc acctgatggg
     4921 tttgtgctgt ctccagcagc cagtcaagaa aagcctgatg ttcagcttcg cggcttttac
     4981 tgtagaagtt ctggccgaga tgccagtctg aggtgtgaag gatgcgcata acggttccct
     5041 ggcgaaaaag catggtcgcg attataccca aacagatgtg ccatttgctt ttttctgcgc
     5101 cacggaaatc aataacctga agatatgtgc gacgagcttt tcataaatct gtcataaatc
     5161 tgacgcataa tgacgtcgca ttaatgatcg caacctattt attacaacag ggcaaatcat
     5221 ggcgagacgt attctggtcg tagaagatga agctccaatt cgcgaaatgg tctgcttcgt
     5281 gctcgaacaa aatggctttc agccggtcga agcggaagat tatgacagtg ctgtgaatca
     5341 actgaatgaa ccctggccgg atttaattct ccttgactgg atgttacctg gcggctccgg
     5401 tatccagttc atcaaacacc tcaagcgcga gtcgatgacc cgggatattc cagtggtgat
     5461 gttgaccgcc agaggggaag aagaagatcg cgtgcgcggc cttgaaaccg gcgcggatga
     5521 ctatatcacc aagccgtttt cgccgaagga gctggtggcg cgaatcaaag cggtaatgcg
     5581 ccgtatttcg ccaatggcgg tggaagaggt gattgagatg cagggattaa gtctcgaccc
     5641 gacatctcac cgagtgatgg cgggcgaaga gccgctggag atggggccga cagaatttaa
     5701 actgctgcac ttttttatga cgcatcctga gcgcgtgtac agccgcgagc agctgttaaa
     5761 ccacgtctgg ggaactaacg tgtatgtgga agaccgcacg gtcgatgtcc acattcgtcg
     5821 cctgcgtaaa gcactggagc ccggcgggca tgaccgcatg gtgcagaccg tgcgcggtac
     5881 aggatatcgt ttttcaaccc gcttttaacg ccttgctcat cggacgcaga gcagggctta
     5941 tgatttctta actggagtat cttacgtgct ggaacggctg tcgtggaaaa ggctggtgct
     6001 ggagctgcta ctttgttgcc tcccggcttt catcctgggt gcattttttg gttacctgcc
     6061 ctggtttttg ctggcatcgg taacaggact gcttatctgg catttctgga atttattgcg
     6121 cctttcatgg tggttgtggg tggatcgcag tatgaccccg ccaccggggc gtggtagctg
     6181 ggaaccgcta ctatacggct tacaccagat gcagctgcga aataaaaaac gccgccgtga
     6241 actgggcaat ctgattaaac gctttcgtag cggcgcggag tcgctgcctg acgcggtggt
     6301 gctgaccacg gaagagggcg gtattttctg gtgtaacggt ctggcgcaac aaattcttgg
     6361 tttgcgctgg ccggaagata acgggcagaa catccttaac ctactgcgtt acccggagtt
     6421 tacgcaatat ctgaaaacgc gtgatttttc tcgcccgctc aatctggtgc tcaacaccgg
     6481 gcggcatctg gaaattcgcg tcatgcctta tacccacaaa cagttgctga tggtggcgcg
     6541 tgatgtcacg caaatgcatc aactggaagg ggcgcggcgt aacttttttg ccaacgtgag
     6601 ccatgagtta cgtacgccat tgaccgtgtt acagggttac ctggagatga tggatgagca
     6661 gccgctggaa ggcgcggtac gcgaaaaagc gttgcacacc atgcgcgagc agacccagcg
     6721 gatggaaggg ctggtgaagc agttgctaac gctgtcgaaa attgaagccg cgccgacgca
     6781 attgctcaat gaaaaggttg atgtgccgat gatgctgcgc gttgttgagc gcgaggctca
     6841 gactctgagt cagaaaaaac agacatttac ctttgagata gataacggcc tcaaagtgtc
     6901 tggcaacgaa gatcagctac gcagtgcgat ttcgaacctg gtctataacg ccgtgaatca
     6961 tactccagaa ggcacgcata tcaccgtacg ctggcagcga gtgccgcacg gtgccgaatt
     7021 tagcgttgaa gataacggac cgggcattgc accggagcat attccgcgcc tgaccgagcg
     7081 tttttatcgc gttgataaag cgcgttcccg gcaaaccggc ggtagcggat tagggttagc
     7141 gatcgtgaaa catgctgtga atcatcacga aagtcgcctg aatattgaga gtacagtagg
     7201 aaaaggaaca cgtttcagtt ttgttatccc ggaacgttta attgccaaaa acagcgatta
     7261 atccgccttt gtcatctttt attgccataa gccagtcgat gctggcttat tttctttgca
     7321 gtcaaaatac gggcgttaga ttttacaacg gttggtgatt ttttgttcgc atgattagcc
     7381 atgtcttttt cacggaaata gtgttttata ctggttggtg atttcttatc gctatatacc
     7441 tctggttttt agatccctcc ttgctttaaa acgttataag cgtttaaatt gcgcttcagg
     7501 tgctgtcata ctgactgcat taacgcggta aatcgaaaaa ctattcttcg ccgcgccagg
     7561 ttgggagtat ttcccgctaa aattgtttaa atataccgct gtatcatccc cagggattgg
     7621 cacaaaaatt taacgttaca acaccacatc cacaggcagt atgatttatg acccatcaat
     7681 taagatcgcg cgatatcatc gctctgggct ttatgacatt tgcgttgttc gtcggcgcag
     7741 gtaacattat tttccctcca atggtcggct tgcaggcagg cgaacacgtc tggactgcgg
     7801 cattcggctt cctcattact gccgttggcc tgccggtatt aacggtagtg gcgctggcaa
     7861 aagttggcgg cggtgttgac agcctcagca cgccaatcgg taaagtcgct ggcgtgctgc
     7921 tggcaacggt ttgttacttg gcggtggggc cgcttttcgc tacgccgcgt acagctaccg
     7981 tttcctttga agtggggatt gcgccgctga cgggtgattc cgcgctgccg ctgtttatct
     8041 acagcctggt ctatttcgct atcgttattc tggtttcgct ctatccgggc aagctgctgg
     8101 ataccgtggg caacttcctt gcgccgctga aaattatcgc gctggtcatc ctgtctgttg
     8161 ccgctattgt ctggccggcg ggttctatca gcacggcgac tgaggcttat caaaacgctg
     8221 cgttttctaa cggcttcgtt aacggctatc tgaccatgga tacgctgggc gcaatggtgt
     8281 ttggtatcgt tattgttaac gcggcgcgtt ctcgtggcgt taccgaagcg cgtctgctga
     8341 cccgttatac cgtctgggct ggcctgatgg cgggtgttgg tctgactctg ctgtacctgg
     8401 cgctgttccg tctgggttca gacagcgcgt cgctggtcga tcagtctgca aacggcgctg
     8461 ctattctgca tgcttacgtt cagcacacat ttggcggcgg cggtagcttc ctgctggcgg
     8521 cgttaatctt catcgcctgc ctggtaacgg cagttggcct gacctgtgct tgtgcagaat
     8581 tctttgccca gtacgtaccg ctctcttatc gtacgctggt gtttatcctc ggcggcttct
     8641 cgatggtggt gtctaacctc ggcttaagcc agctgatcca gatctccgta ccggtgctga
     8701 ccgctattta tccgccgtgt atcgcactgg ttgtattaag ttttacacgc tcatggtggc
     8761 ataattcgtc ccgcgtgatt gccccgccga tgtttatcag cctgcttttt ggtattctcg
     8821 acgggatcaa agcatctgca ttcagcgata tcttaccgtc ctgggcgcag cgtttaccgc
     8881 tggccgaaca aggcctggcg tggttaatgc caacagtggt gatggtggtt ctggccatta
     8941 tctgggatcg cgcggcaggt cgtcaggtga cctccagcgc tcactaaatc actgaacatt
     9001 tgttttaacc acggggctgc gatgccccgt ggttttttat tgtgttgatg ggttaggaat
     9061 tgatggaaag taagaacaag ctaaagcgtg ggctaagtac ccgccacata cgctttatgg
     9121 cactgggttc agcaattggc accgggctgt tttacggttc ggcagacgcc atcaaaatgg
     9181 ccggtccgag cgtgttgttg gcctatatta tcggtggtat cgcggcgtat atcattatgc
     9241 gtgcgctggg ggaaatgtcg gtacataacc cggccgccag ctctttctcg cgttatgcgc
     9301 aggaaaacct cggcccgctg gcaggttaca ttaccggctg gacctactgc tttgaaatcc
     9361 taattgtcgc catcgccgat gtgaccgctt ttggtatcta tatgggtgtc tggttcccga
     9421 cggtgccgca ctggatttgg gtactgagcg tggtgctgat catttgcgcc gtaaacctga
     9481 tgagcgtgaa ggtattcggt gagctggaat tctggttctc gttctttaaa gtcgccacca
     9541 tcatcatcat gattgtcgcc ggtttcggca tcatcatctg ggggattggc aacggcgggc
     9601 aaccgaccgg tattcataac ctgtggagca acggcggctt cttcagtaac ggctggcttg
     9661 gcatggtaat gtcgttgcaa atggtgatgt ttgcttacgg tgggatcgaa attatcggga
     9721 ttaccgccgg tgaagcgaaa gatcctgaga aatcgattcc gcgtgcgatt aactccgtgc
     9781 cgatgcgtat tctggtgttc tacgtcggta cgctgttcgt cattatgtct atctacccgt
     9841 ggaatcaggt tggcactgcc ggtagcccgt tcgtgctgac gttccagcat atgggcatta
     9901 cctttgccgc cagcattctt aactttgttg tgctgaccgc ttcgctgtcg gcaattaaca
     9961 gtgacgtatt tggcgtaggc cgtatgctcc acggtatggc agagcagggc agcgcaccga
    10021 aaattttcag caaaacgtcg cgtcgcggta ttccgtgggt tacggtgctg gtgatgacta
    10081 ccgcgctgct gtttgcggtg tatctgaact acatcatgcc ggaaaacgtc ttcctggtga
    10141 tcgcttcgct ggcaaccttc gccacggtat gggtgtggat tatgatcctg ctgtcgcaaa
    10201 tcgccttccg tcgccgtttg ccgccagaag aagttaaggc gctgaaattt aaagtgccgg
    10261 gtggggtagc aacgaccatc ggcgggctga ttttcctgct ctttattatc gggttgattg
    10321 gttatcaccc ggatacgcgt atctcgctgt atgtcggttt cgcgtggatt gttgtgctgt
    10381 tgattggctg gatgtttaag cgtcgccacg atcgtcagct ggctgaaaac cagtaatcct
    10441 tgcgttctgt aagccggttc aggaggaaat cctgatccgg ctattccgaa agttatccgc
    10501 ccccgtcctc ctcccccaaa tatccttcag atgatgagtg atcctgcatt aggctatggc
    10561 aaggtgatca gattttcatc acaggggaat tatgatgtta aatgcatggc acctgccggt
    10621 gcccccattt gttaaacaaa gcaaagatca actgctcatt acactgtggc tgacgggcga
    10681 agacccaccg cagcgcatta tgctgcgtac agaacacgat aacgaagaaa tgtcagtacc
    10741 aatgcataag cagcgcagtc agccgcagcc aggcgtcacc gcagggcttc agccgaatgc
    10801 cgccggcacg actggagcag tttgccgtcg atgtaccgga tatcggccca caatgggctg
    10861 cggatcagat tttttatcag atcttccctg atcgttttgc gcgtagtctt cctcgtgaag
    10921 ctgaacagga tcatgtctat taccatcatg cagccggaca agagatcatc ttgcgtgact
    10981 gggatgaacc ggtcacggcg caggcgggcg gatcaacgtt ctatggcggc gatctggacg
    11041 ggataagcga aaaactgccg tatctgaaaa agcttggcgt gacagcgctg tatctcaatc
    11101 cggtgtttaa agctcccagc gtacatcaat acgataccga ggattatcgc catgtcgatc
    11161 cgcagtttgg cggtgatggg gcgttgctgc gtttgcgaca caatacgcag cagctgggaa
    11221 tgcggctggt gctggacggc gtgtttaacc acagtggcga ttcccatgcc tggtttgaca
    11281 ggcacaatcg tggcacgggg ggagcttgtt acaaccctga atcgccctgg cgcgactggt
    11341 actcgtttag tgatgatggc acggcgctcg actggcttgg ctatgccagc ttgccgaagc
    11401 tggattatca gtcggaaagt ctggtgaatg aaatttatcg cggggaagac agtattgtcc
    11461 gccattggct gacagcgccg tggagtatgg acggctggcg gctggatgtg gtgcatatgc
    11521 tgggggaggc gggtggggcg cgcaataata tgcagcacgt tgctgggatc accgaagcgg
    11581 cgaaagaaac ccagccggaa gcgtatattg tcggcgaaca ttttggcgat gcacggcaat
    11641 ggttacaggc cgatgtggaa gatgccgcca tgaactatcg tggcttcaca ttcccgttgt
    11701 ggggatttct tgccaatacc gatatctctt acgatccgca gcaaattgat gcccaaacct
    11761 gtatggcctg gatggataat taccgcgcag ggctttctca tcaacaacaa ttacgtatgt
    11821 ttaatcagct cgacagccac gatactgcgc gatttaaaac gctgctcggt cgggatattg
    11881 cgcgcctgcc gctggcggtg gtctggctgt tcacctggcc tggtgtaccg tgcatttatt
    11941 acggtgatga agtgggactg gatggtaaaa acgatccgtt ttgccgtaaa ccgttcccct
    12001 ggcaggtgga aaagcaggat acggcgttat tcgcgctgta ccggcgaatg attgtgctgc
    12061 gtaagaaaag tcaggcgctg cgtcgtggcg gctgtcaggt gctgtatgcg gaagataacg
    12121 tagtggtatt tgtccgcgtg ctgaatcagc aacgtgtact ggtggcaatc aaccgtggcg
    12181 aggcctgtga agtggtgcta cccgcgtcac cgtttctcaa tgccgtgcaa tggcaatgca
    12241 aagaagggca tgggcaactg actgacggga ttctggcttt gcctgccatt tcggctacgg
    12301 tatggatgaa ctaacgtttt ataacgcctt gtgtgacgcc tgcgccatca tccgcggata
    12361 aaactgccag aagcgggttc cgagggcggt ataatgggcg tttaaatcgt accaggagtc
    12421 acgcagggca tccagacgtg ggcgacggct tgccatgccg tttaacacgt tctggatgaa
    12481 atccatatcg cgatagcgca ccagccactg ctctgaccac aagtaattgt ttagattgat
    12541 aaaacgtggc ggtgagtccg gcaaaatcgt catcacttgc ttgcgggcat aacagataaa
    12601 ttcctgtagc ggaaagtccg gcgacagctg cgaccagtgg cgggaaagaa agtgatccca
    12661 catgacatcc agcgtaatag gcgcaacgcg gcgcgtttca cgacgaaacc actcccgtgc
    12721 ttcgcggact tccggcagat tgtcagtcaa tacgtcgata cgtcgataca tatgaatgcc
    12781 agccacgacg tcgggcggaa aactttcttc aggatttccg cgtacgaaat cagccagtaa
    12841 attgccggaa agcgagcttt ccgcgagatg ggctaaatgc aggtgagcta aaaaattcat
    12901 cgattatatt ctatccaaag gggggtaaag gttgcaggga gagcgccccg gcactagact
    12961 acccgcctct tattttagtc tgagtcagtg tcatgcgcgt taccgatttc ttctttgaat
    13021 tgcccgaatc cctgattgcc cactacccca tgcctgaacg cagtagctgt cgtttactgt
    13081 cgctggacgg gcctacgggc gcgctgacgc acggtacttt caccgattta ctcgataagc
    13141 tcaaccccgg cgatcttctg gtttttaata atactcgcgt gatcccggcg cgcctgtttg
    13201 ggcgtaaagc cagcggcggc aagattgaag tgctggttga gcggatgctc gacgacaaac
    13261 gcattcttgc gcatattcgc gcctcgaaag cgccaaaacc tggcgcagaa ctgctgctgg
    13321 gcgatgacga aagtattaac gcaacaatga ccgcgcgcca cggcgcactg tttgaagtcg
    13381 aattcaatga tgatcgttcg gtactggata tcctcaacag tatcgggcat atgccgctgc
    13441 cgccgtatat cgaccgtccg gacgaagacg ctgaccgcga actttatcaa accgtttata
    13501 gcgaaaaacc gggcgcggtt gcagccccga ccgctggtct gcattttgac gagcctttgc
    13561 tggaaaaatt gcgcgccaaa ggcgtggaga tggcgtttgt gacgttgcac gttggtgcgg
    13621 gcaccttcca gccggtgcgc gtcgacacca ttgaagatca catcatgcac tcggaatacg
    13681 ctgaagtacc gcaggatgtg gtagacgcgg tactggcggc gaaagcgcgc ggtaaccggg
    13741 tgattgcggt tggcaccact tcagtacgtt cgctggaaag cgcggctcag gcagcgaaaa
    13801 acgatctcat tgaaccgttc ttcgacgata cccaaatctt tatctatccg ggcttccagt
    13861 acaaagtggt cgatgcgctg gtgacgaact tccacttgcc agagtcgacg ctgattatgc
    13921 tggtttcggc ctttgccggt tatcaacaca ccatgaacgc ctataaagca gcggtagaag
    13981 agaaatatcg cttttttagt tacggtgatg cgatgtttat tacgtacaat ccgcaggcaa
    14041 ttaatgagcg cgtcggggag taattccgcg gcgctggttt aaaacgttgg actgtttttc
    14101 tgacgtagtg gagaaaaaat gaaatttgaa ctggacacca ccgacggtcg cgcacgccgt
    14161 ggccgcctgg tctttgatcg tggcgtagtg gaaacgcctt gttttatgcc tgttggcacc
    14221 tacggcaccg taaaagggat gacgccggaa gaagttgaag ccactggcgc gcaaattatc
    14281 ctcggcaaca ccttccacct gtggctgcgc ccgggccagg aaatcatgaa actgcacggc
    14341 gatctgcacg attttatgca gtggaagggg ccgatcctca ccgactccgg gggcttccag
    14401 gtcttcagcc ttggcgatat tcgtaaaatc accgaacagg gcgtgcactt ccgtaacccg
    14461 atcaacggcg atccaatttt cctcgatcct gaaaaatcaa tggagattca gtacgatctt
    14521 ggttcggata tcgtcatgat ctttgatgag tgtacgccgt atcctgctga ctgggattac
    14581 gcaaaacgct ccatggagat gtctctgcgt tgggcgaagc gtagccgtga gcgttttgac
    14641 agtctcggaa acaaaaatgc gctgtttggt atcattcagg gcagcgttta cgaagattta
    14701 cgtgatattt ctgttaaagg tctggtagat atcggttttg atggctacgc tgtcggcggt
    14761 ctggctgtgg gtgagccgaa agcagatatg caccgcattc tggagcatgt atgcccgcaa
    14821 attccggcag acaaaccgcg ttacctgatg ggcgttggta aaccagaaga cctggttgaa
    14881 ggcgtacgtc gtggtatcga tatgtttgac tgcgtaatgc caacccgcaa cgcccgaaat
    14941 ggtcatttgt tcgtgaccga tggcgtggtg aaaatccgca atgcgaagta taagagcgat
    15001 actggcccac tcgatcctga gtgtgattgc tacacctgtc gcaattattc acgcgcttac
    15061 ttgcatcatc ttgaccgttg caacgaaata ttaggcgcgc gactcaacac cattcataac
    15121 cttcgttact accagcgttt gatggcgggt ttacgcaagg ctattgaaga gggtaaatta
    15181 gagagcttcg taactgattt ttaccagcgt caggggcgag aagtaccacc tttgaacgtt
    15241 gattaatatt aataatgagg gaaatttaat gagctttttt atttctgatg cggtagcggc
    15301 aacgggtgca ccggcgcaag gtagcccgat gtctttgatt ttgatgctgg tggtattcgg
    15361 tctgattttc tatttcatga tcctgcgtcc acagcagaag cgcaccaaag aacacaaaaa
    15421 gctgatggac tccattgcca aaggtgatga agttctgacg aacggtggcc tggttggtcg
    15481 cgtaaccaaa gtagcggaaa acggctacat tgctatcgcg ctgaatgaca ccactgaagt
    15541 agttattaaa cgtgacttcg tagctgccgt cctgccgaaa ggcaccatga aggcgctgta
    15601 attaaaattt ttccctaagg gaattgccgt gttaaaccgt tatcctttgt ggaagtacgt
    15661 catgctgatc gtggtgattg tcatcggtct gctgtatgcg cttcccaacc tgtttggtga
    15721 ggatccggct gttcagatca ctggtgcgcg cggagtcgcc gccagtgagc aaacgctgat
    15781 ccaggtccag aaaacgttac aagaagaaaa aataactgct aagtctgtgg cactggaaga
    15841 gggcgctatt cttgcgcgct tcgactccac tgacacccag ctgcgcgctc gtgaagcatt
    15901 aatgggcgtt atgggtgaca aatacgtcgt ggcgcttaac cttgccccgg caacgccgcg
    15961 ctggctggca gctattcacg ctgagccgat gaagctcggc cttgacctgc gtggcggcgt
    16021 tcacttcctg atggaagtgg atatggacac cgcgcttggc aaactccagg aacaaaatat
    16081 cgatagcctg cgcagcgatc tgcgtgaaaa gggcatcccg tataccaccg ttcgtaaaga
    16141 aaacaactac ggcctgagca ttactttccg cgatgctaaa gctcgtgatg aagccattgc
    16201 gtatctgagc aagcgccatc cggacctggt gattagtagc cagggcagca accagctgcg
    16261 tgcggtaatg agcgatgctc gtctgagtga agcgcgtgaa tatgcggtgc agcagaacat
    16321 caatatcctg cgtaaccgtg taaaccaact tggcgtagcg gagccggtgg ttcagcgtca
    16381 gggtgctgac cgtatcgttg ttgaactgcc aggtattcag gacactgcgc gtgcgaaaga
    16441 gattctgggt gcgacggcaa cgctggaatt ccgtctggta aacaccaacg ttgaccaggc
    16501 cgctgcggca tccggtcgcg taccgggcga ctctgaagtg aaacagaccc gcgaaggtca
    16561 gccagttgtg ctgtacaaac gcgtaattct gaccggtgac catatcaccg actccacttc
    16621 cagccaggac gaatacaacc aaccgcaggt taacatctcg ctcgatagcg ctggtggtaa
    16681 catcatgtct aacttcacta aggacaacat cggcaaaccg atggcaaccc tgtttgtgga
    16741 gtacaaagac agcggtaaga aagatgcaaa tggtcgtgcg gttctggtga aacaggaaga
    16801 ggtgattaac atcgccaaca tccagtctcg tctgggtaac agcttccgta tcaccggcat
    16861 caacaacccg aacgaagccc gtcagctgtc actgctgctg cgtgccggtg cgttgatcgc
    16921 gccgattcag attgttgaag aacgtaccat tggcccaacc ctgggtatgc agaacattga
    16981 acaggggctg gaggcttgcc ttgccggtct gctggtttct attctgttca tgatcatctt
    17041 ctataagaag tttggtctga ttgcgaccag tgctctgatt gccaacttga tcttaatcgt
    17101 cggcattatg tcgctgttgc caggcgcaac gctgagtatg ccaggtatcg cgggtatcgt
    17161 cttaaccctt gcggtggcgg tcgatgcgaa cgtactgatc aacgaacgta ttaaagaaga
    17221 gttgagcaac ggacgtactg ttcaacaggc aattgatgaa ggttatcgtg gcgcattcag
    17281 ttctatcttc gatgcgaaca tcaccacgct gattaaagtc atcatcctgt acgcagtggg
    17341 taccggggca attaaagggt tcgcgattac taccggtatc ggtgtggcga cgtcgatgtt
    17401 taccgcgatt gtcggtacgc gtgccatcgt aaacctgcta tatggcggca agcgcgtcaa
    17461 gaagctgtca atctgaggag tgcgatgtgg cacaggaata tactgttgaa caactaaacc
    17521 acggccgtaa agtctatgac tttatgcgct gggactactg ggctttcggc atctctggtc
    17581 tgctgttaat cgccgctatc gttattatgg gcgtgcgcgg ctttaactgg gggctggatt
    17641 tcaccggtgg tacggttatc gaaattacgc tcgaaaaacc ggctgaaatt gacgtaatgc
    17701 gtgatgcatt gcaaaaagcc ggttttgaag agccaatgct gcaaaacttt ggtagcagcc
    17761 acgacatcat ggtccgtatg ccgcctgctg aaggcgaaac cggcggtcag gtgctgggca
    17821 gccaggttct gaaggtgatt aacgaatcca ccaatcagaa tgcagcagtg aagcgtattg
    17881 agttcgtcgg tccgagcgtg ggggcagacc ttacgcaaac cggtgcgatg gcgttgatgg
    17941 cggcgctgct gtctatcctt gtgtacgtag gttttcgctt tgagtggcga ctggcggcag
    18001 gggttgttat tgcgctggcg cacgacgtta tcattacgct gggtattttg tcgttattcc
    18061 atatcgagat tgacctgacc attgtggcat cgttgatgtc ggttatcggt tactcgctta
    18121 acgacagtat cgtggtatcg gaccgtattc gtgaaaactt ccgcaagatc cgtcgcggta
    18181 cgccttacga aatctttaac gtgtccttga cccagacgct gcaccgtacc ttgatcacat
    18241 ccggtactac cttgatggtt atcctgatgc tgtacctctt cggtggtccg gtactggaag
    18301 gcttctcgct gaccatgctt atcggtgttt ctatcggtac tgcatcttcc atctatgtgg
    18361 catctgcgtt ggctctgaaa ctgggtatga agcgcgaaca catgttgcag cagaaagtgg
    18421 aaaaagaagg ggcggatcag ccgtcaattc tgccgtaatc aagttcccgt tgatgttgaa
    18481 aatcccggtc agaagatcgg gatttttttt gatgtatgga gattgcgaaa atccccgcat
    18541 cttgggaaac tgcgcgtaac cctacatttc atccaggtaa ctcttcatgg ctatcatccc
    18601 aaaaaactat gcgcggttag aaagcggcta tcgcgaaaaa gcattaaaaa tatatccgtg
    18661 ggtctgcggt cgctgttccc gcgagtttgt ttattccaac ctgcgtgaac ttaccgttca
    18721 ccacattgat cacgaccata ccaataaccc ggaagatggc agtaactggg aattgctttg
    18781 tctctattgc cacgatcatg agcattcgaa atataccgaa gcggatcagt atggtacgac
    18841 cgttatcgca ggggaagatg cgcagaaaga tgtcggtgaa gcgaagtaca acccattcgc
    18901 tgacctgaaa gcgatgatga acaagaagaa gtgattaaaa cgtaaaattg cctgatgcgc
    18961 tacgcttatc aggcctacgt tatttcagca atatattgaa tttacgtgct tttgtgggcc
    19021 ggacaaagcg tttacgccgc atccggcatg aacaaagctc acgttgttaa caatcagaaa
    19081 tgccgggaat aaatcccggc attttcataa tcagaagttg taacctacta ccaggtaacc
    19141 accccagccg gtagagcgaa cgttgaagtt gccgttgccg aagttcagtt ctgcatcgtc
    19201 gttccactga ccaccgtcgt gccagtaacg agctacgaca gagtagtgcc agtgatcgta
    19261 gttcagagcc agaatatggc tggaagcgat agagttatta gtacgggtct taataccgtt
    19321 gattgcgtta ccgctgtcat cccctaaatc ggaaccccag tcgaagttgg tgaagccgat
    19381 gtagctcagc tgaccgcccc acagatcggt aatcggcaca aagtatttaa ttttgaaacg
    19441 gtaaccgtcc cactcgtttt cgttcgctgc gccatagttc tgccactggt atttcgcata
    19501 gacgttcatg gacaggctca tcggcagacc agtatcgatg tcggtaccca gacccatgta
    19561 ccaggtgctc tggcgaccat ttttattacg acccatgtcg taaatgtagt tgttcgcgaa
    19621 gtaccactct ttgaacggac cgaagttaag gtcagtattg gtcagcttgt cgatggagaa
    19681 acgtggttcg atttccataa acagcggaga accgtggttc cagatacctt tagcatcgga
    19741 gttaccgccg aagaataccg gcgcatccgc ataaccatag aagtcgaacc agtctttttt
    19801 agcgaatgct tcgtactcaa ggtaggtatc gttgcggatc tgcggtccga aacgggtgtg
    19861 atagcttccg acaacgttaa cgctctggtg ccaccagtcg gaaagatact gcggtttgtc
    19921 gttttcagct gcgttgacag taaaagacga agagagcgcc agtaccgcac cggctgccag
    19981 taatgttttt ttcatatgta tgccactgtt tgaaaatccc ttgcgggagt gaaaaaggcg
    20041 caaattgcgt ttctaaatat ttcgtataca gaggagccta ttataaaaat cattgctcac
    20101 aaaaatatgt ttcgtttcac agttctatca tttacgtaat cgattgcgtt cacgtttaca
    20161 cacattcggg cggggattgt actgactttc gcccttgttg caaactttta ctatcaatcc
    20221 aggtgatgta agcagagtaa caaaatgacg gggaaagatg ttgcgggacg cacaaaatcg
    20281 tcccgaaata cgtttactgc ataacaggtt gaatgtcgtg gatacgtaca aagcctaact
    20341 gatccacaga aatccccttt aattgtaacg gaatatcaac atcgctgggg gcgaggacgc
    20401 tggcaggggc attaatcaat tgattctgca cattgatttc ctgaaagttg tctattgttc
    20461 cctgaatttg cccatattca acagttccgc tgaatgcggg caagggatca ttcgactcac
    20521 cctgaatacg tagtgtaagg ggggtgccat ctgtatcagg cgtaatattc accagcgaca
    20581 tacgtaaagt accgatctgg ctttccagtc ttgccggtgt tttcgctcca ggcagaaggt
    20641 aacccccact gctggatttt gcattcagcc tgttttgctg agtgattttg actgtttctt
    20701 gattgagttg cgtcatctcc tggttcagcg tgctgacgct gtgtttcatc tgtcgcactt
    20761 cactttgctg cacacaggcg ctaaggctga acaggcttcc cagcaggagc agtcgaaaaa
    20821 cgttagtatt cataggtcgt ccctttctcg tcgcaattag cctaatggta gagggtaaaa
    20881 gcagcgaaag catcgatcct ttatctcaaa agcgttgcgc ctttgttgta tcgtcagttc
    20941 agggtaaaat agatttccgt taaccacctg gtcaggacgc cgtatgcatt gcccattctg
    21001 tttcgccgtg gacactaagg taattgactc tcgtctcgtg ggcgagggtt catccgtacg
    21061 ccgccgtcgg cagtgtctgg tgtgtaatga acgtttcacc acctttgaag tggcggagct
    21121 ggttatgccg cgtgttgtaa aaagcaacga cgtgcgtgaa ccgtttaatg aagagaaatt
    21181 gcgtagcgga atgctgcggg cgctggaaaa acgtccggtg agttccgatg acgttgaaat
    21241 ggcaatcaat catattaaat cgcagctgcg cgccaccggt gagcgcgaag taccgagcaa
    21301 gatgattggc aatctggtga tggagcaatt gaaaaagctc gataaagtcg cctatatccg
    21361 ttttgcctct gtctaccgca gtttcgaaga tatcaaagaa tttggcgaag agatcgcgcg
    21421 cctggaggac taagccgtgc aggacgagta ttacatggcg cgggcgctaa agctggcgca
    21481 acgaggacgt tttaccaccc accccaaccc aaatgtcggg tgcgtcattg tcaaagatgg
    21541 cgaaattgtc ggtgaaggtt accatcaacg agcgggtgaa ccacatgccg aagtacacgc
    21601 gttgcgtatg gcgggtgaaa aagccaaagg tgcaacggcg tatgtgacac tcgaaccttg
    21661 cagccatcac ggccgtacgc cgccttgctg tgatgcttta attgccgcag gtgtggcgcg
    21721 cgtggttgcc tcgatgcaag atcctaaccc gcaggtcgct gggcgtggac tttaccgtct
    21781 gcaacaggct ggcattgacg tcagccacgg cctgatgatg agtgaagccg agcaattgaa
    21841 taaaggcttt ctcaagcgga tgcgcaccgg ctttccttat attcagttaa aacttggcgc
    21901 atcgcttgat ggtcgcacgg cgatggcgag cggcgaaagc cagtggatca cttcgcccca
    21961 ggcgcggcgt gatgtacaac gactgcgcgc gcaaagtcat gccattttaa ccagcagcgc
    22021 cacggtgctg gcggatgatc ctgccttaac ggtgcgttgg tccgaactgg atgaacaaac
    22081 tcaggcgctc tacccgcaac aaaatctccg ccagccggta cgtattgtga ttgatagcca
    22141 aaatcgcgtg acgccggaac atcacattgt gcagcagccc ggcgaaacct ggttcgcgcg
    22201 tacgcaggaa gattctcgtg agtggccgga aacggtgcgt accttgctga ttccagagca
    22261 taaaggtcat ctggatctgg ttgtactgat gatgcaactg ggtaaacagc agattaacag
    22321 catctggggg gaagcggggc caacgctcgc tggcgcattg ctgcaggcgg gtttagtcga
    22381 tgagctgatt gtctatatcg cacctaaact attaggcagc gacgcccgtg gattatgcac
    22441 cctgccaggg cttgagaaat tagccgacgc cccccaattt aaattcaaag agatacgtca
    22501 tgtaggcccg gatgtttgcc tgcatttagt gggtgcatga tctcccggct cgaaagggaa
    22561 gcagcgcacg aaatattatg ctaaaatccg cccccctgcg gggccatact cgaacccgaa
    22621 ggaagaaaat gaacattatt gaagctaacg ttgctacccc ggacgctcgc gtcgccatca
    22681 ccatcgcgcg tttcaacaac tttatcaatg acagcctgct ggaaggtgca attgacgcac
    22741 tgaaacgtat cggtcaggta aaagatgaaa acattaccgt tgtttgggtg cctggtgcct
    22801 atgagctgcc gctggcggcg ggtgcactgg ctaaaaccgg taaatacgac gcggtgattg
    22861 cgctgggtac ggttattcgt ggtggcactg cccattttga atatgtcgct ggtggtgcaa
    22921 gcaacggcct ggcgcatgtt gcccaggaca gcgaaattcc ggttgctttt ggggttctga
    22981 ccactgaaag cattgaacaa gcgatcgaac gtgctggcac caaagctggc aacaaaggtg
    23041 cagaagctgc actgaccgcg cttgaaatga ttaatgtatt gaaagccatc aaggcctgaa
    23101 attagtaagg ggaaatccgt gaaacctgct gctcgtcgcc gcgctcgtga gtgtgccgtc
    23161 caggcgctct actcctggca gttgtcccag aacgacatcg ctgatgttga ataccagttc
    23221 ctggctgaac aggatgtaaa agacgttgac gtcctgtact tccgtgagct gctggccggg
    23281 gtggcgacta ataccgcata cctcgacgga ctgatgaagc catacctgtc ccgtctgctg
    23341 gaagaactgg gacaggtaga aaaagcagta ctgcgcattg cgctgtacga actgtctaaa
    23401 cgtagcgatg tgccatacaa agtggccatt aacgaagcga tcgaactggc gaaatcgttc
    23461 ggcgcagaag acagccataa gttcgtcaac ggtgtactcg ataaagcagc acctgtgatt
    23521 cgccctaaca aaaagtaata tccaggccgg tagattcacg gaagaccgtt ccatgatcgc
    23581 cggccttttc ttttttacct gctgaggcat aacgtatggc atgtggcgag ttctccctga
    23641 ttgcccgtta ttttgaccgt gtaagaagtt ctcgtcttga tgtcgaactg ggcatcggcg
    23701 acgattgcgc acttctcaat atccccgaga aacagaccct ggcgatcagc actgatacgc
    23761 tggtggcggg caaccacttc ctccctgata tcgatcctgc tgatctggcg tataaagcac
    23821 tggcggtgaa cctaagcgat ctggcagcga tgggggccga tccggcctgg ctgacgctgg
    23881 cattaacctt gccggacgta gacgaagcgt ggcttgagtc cttcagcgac agtttgtttg
    23941 atcttctcaa ttattacgat atgcaactca ttggcggcga taccacgcgt gggccattat
    24001 caatgacgtt gggtatccac ggctttgttc cgatgggacg agccttaacg cgctctgggg
    24061 cgaaaccggg tgactggatc tatgtgaccg gtacaccggg cgatagcgcc gccgggctgg
    24121 cgattttgca aaaccgtttg caggttgccg atgctaaaga tgcggactac ttgatcaaac
    24181 gtcatctccg tccatcgccg cgtattttac aggggcaggc actgcgcgat ctggcaaatt
    24241 cagctatcga tctctctgac ggtctgattt ccgatctcgg gcatatcgtg aaagccagcg
    24301 actgcggcgt acgtattgac ctggcattgc tgccgttttc tgatgcgctt tctcgccatg
    24361 ttgaaccgga acaggcgctg cgctgggcgc tctctggcgg tgaagattac gagttgtgtt
    24421 tcacggtgcc ggaactgaac cgtggagcgc tggatgtggc tctcggacac ctgggcgtac
    24481 cgtttacctg tatcgggcaa atgaccgccg atatcgaagg gctttgtttt attcgtgacg
    24541 gtgaacctgt tacgttagac tggaaaggat atgaccattt tgccacgcca taaagatgtc
    24601 gcgaaaagtc gcctgaagat gagtaatccg tggcatctac ttgctgtcgg attcggaagt
    24661 ggattaagcc cgatcgttcc tgggacgatg ggctcgctgg cagcgattcc gttctggtat
    24721 ctgatgacct ttttgccctg gcagctctac tcgctggtgg tgatgctggg gatctgtatc
    24781 ggcgtctatc tttgtcatca gaccgcaaaa gatatgggcg tgcacgatca cggcagcatt
    24841 gtctgggacg aatttattgg tatgtggatc acgctcatgg cgctgccgac caatgactgg
    24901 cagtgggttg ccgccgggtt tgtgattttc cgtattctgg atatgtggaa accgtggccg
    24961 atccgctggt ttgatcgcaa tgtgcatggc ggcatgggga tcatgataga tgatattgtc
    25021 gccggggtga tctccgctgg catcctgtac tttattggtc atcactggcc gctgggcatt
    25081 ctgtcgtagt tgtgcaccga tgcctgatgt gacgcttgtt gcgtctcatc aggcctggac
    25141 tcttatttaa atcctactac cggatgcggt ttatacggcg tttccagttc ggcaatctgt
    25201 tccggcttca aagtgatatc caccgcgttc aatagctcat caagctgttc ttcccgcgat
    25261 gtaccgataa tcggtgcggc aatgcccggt ttactcaaca accaagccag cgcaacttgt
    25321 gctcgtgttg caccgagttc ttcactgacg cccgttaacc gctctgcgat ctgcgcgtca
    25381 ttttcatcgc tttctttata gagatttttc cccacctcat cagacaccag tcgtgcggta
    25441 gtttctcccc acggacgcgt cagtcgcccc cgcgccagcg ggctccacgg aatcaccgcc
    25501 acgccctcct gataaaacag tggcagcatc tcgcgctctt cttcgcgata aatcagattg
    25561 tagtgatcct gcatactgac aaactgcgcc cagccgtgct gtttttggag ttccagtgcc
    25621 tgagcaaact gcgaagcgtg cattgatgac gcgccgatat aacgcgcttt cccggctttt
    25681 accacgtcgt tcagggcttc cagcgtctct tcgatcggcg tgttgtaatc ccagcgatga
    25741 atttgcagga tatcgacata atccatgccg agacgtcgca ggctgtcgtc gatagagcgc
    25801 aaaatttgtg cacgggataa tccttccggt aaatcaccaa cgcgatggaa cactttggtg
    25861 gcaacgacca cgtcttcacg acgggcgaaa tcccgcagtg cgcgaccaac gatctcttcg
    25921 ctgctaccat cggaatagct attggcggta tcaaagaaat ttatgccgcc ttccagtgcg
    25981 cgtttaatta tgggacggct gctctcttcc ggcagtgtcc atgcgtgatt accacgatct
    26041 ggctcgccaa aggtcataca gccgaggcaa agtcgggaaa cgcgaaggtc ggtttttcct
    26101 aaggggttgt attgcatgct gccactcctg ccatgaataa tttcttaagc atagcaggag
    26161 tggagtaggg attatgccag ccaggccttg attttggctt ccataccggc ggcatcgagg
    26221 ccgagttcgg cgcgcatttc ttcctgcgtt ccttgcggaa taaagaagtc aggcaggcca
    26281 atgttcagca cggggactgg tttacgatgg gccattagca cttcgttcac tccgctgcct
    26341 gcaccgccca taatggcgtt ttcttctacg gtaaccagtg cttcatgact ggcggccatt
    26401 tccagaatta acgcttcatc aagcggtttc acaaaacgca tatcgaccag cgtggcgttc
    26461 agcgattcgg cgactttcgc cgcttctggc atcagcgtac caaagttaag gatcgccagt
    26521 ttctcgccac gacgcttcac aatgcctttg ccaattggta gtttttccag cggcgtcagt
    26581 tccacgccga ccgcgttgcc acgcgggtag cgcaccgctg acgggccatc gttatagtga
    26641 tagccggtat agagcatctg gcgacattcg ttttcatcgc tcggggtcat aatgaccatt
    26701 tccggtatgc agcgcaggta agagagatca aaagcaccct gatgggtttg accgtcagca
    26761 ccaacaatgc ccgcgcggtc gatggcgaac aggaccggaa gcttttgaat tgccacgtca
    26821 tgcagcacct gatcataggc gcgttgcagg aaagtggagt agatcgcgac aatgggtttg
    26881 tacccaccaa tcgccagacc ggcggcaaag gtcactgcgt gttgctcggc aattgccacg
    26941 tcgaagtagc gatccgggaa tttacgagaa aactcgacca tgccggaacc ttcacgcatc
    27001 gccggagtaa tcgccatcag cttgttatct ttcgccgccg tttcgcacaa ccagtcgcca
    27061 aagatttttg aatagctcgg taaaccgccg ctacttttcg gcaaacaacc gctggaggga
    27121 tcaaatttag gcacggcgtg gaaagtgatt gggtcttttt ctgccggttc ataaccacga
    27181 ccttttttgg tcatgatatg caggaactgc gggcctttca ggtcacgcat gttcttcagc
    27241 gtggtgataa gccccagcac atcgtgaccg tcaaccgggc cgatgtagtt aaagcccagc
    27301 tcttcaaaca acgtgccagg gactaccatg cctttaatat gttcttcggt acgtttgagc
    27361 agctctttaa tgggtggcac gccagagaaa acttttttcc cgccttcgcg cagtgaagag
    27421 taaagcttac cggaaagcag ctgcgccaga tggttgttga gcgcgccgac attttcggaa
    27481 atcgacattt cgttgtcgtt gaggaccacc agcatatcag gacggatatc gcccgcgtga
    27541 ttcatcgctt caaacgccat gcctgcggta atcgcgccat cgccgattac acagacggta
    27601 cggcgatttt tgccttcttt ctcggcagca accgcaatac caattccggc actgatggag
    27661 gttgatgaat gcccgacgct taatacgtca tattcgcttt cgccgcgcca cgggaacggg
    27721 tgcaggccgc ctttctgacg gatggtgccg attttgtcgc ggcgtccggt caaaatttta
    27781 tgcggataag cctgatgccc cacatcccaa atcaattggt caaacggggt gttgtagaca
    27841 tagtgcagcg ccacggtcag ttcgaccgtg cccagcccgg aggcgaagtg cccgctggaa
    27901 cggctcacgc tgtcgagtaa atagcggcgc agttcgtcgc agagtttcgg taaactctct
    27961 ttcggcaaca gtcgtaactc ctgggtggag tcgaccagtg ccagggtcgg gtatttggca
    28021 atatcaaaac tcatcagggg cctattaata cttattgttt atttattacg ctggatgatg
    28081 tagtccgcta gcgcttccag tgccgaggta tcgggtgact gttcagccag ttgtttcagc
    28141 gactgacggg catcgtcgat cagatcccgg gctttcttcc gggcttgctc aagacccaga
    28201 agtgcagggt aggtactttt accaagttgc tggtcggcac cctggcgttt tcccaacgtt
    28261 gcagtatctc ctaccacatc caggatgtca tcctgaacct ggaaggcaag gccgatgctc
    28321 tctgcgtact tgtcgagtac tggcagagca cgacgccctt tatctccggc gcttaatgca
    28381 ccaagacgaa cggcggcgcg aatcaatgcg ccggttttat gacgatgaat acgctcaagc
    28441 gcgtccagag gtacgtgttt gccttccgcg tctaaatcta atgcctgacc accgcacatt
    28501 ccggcaatac cgctggcgct cgccagttca gaaatcatcg aaattctgtc gcgatccgac
    28561 acttccggca tatcagcatc gcttaaaatc gagaacgcca gcgtttgtaa agcgtcgcca
    28621 gcgagaatcg cgtttgcttc gccaaacttc acatggcagg tcggcaaacc gcgacgcaga
    28681 tcgtcatcat ccatcgccgg taaatcatca tgaattaatg agtaagcgtg gatacactct
    28741 acggcggcag cgggtgcgtc cagcgtgttt gtgctaacgc caaacatatg accggtggca
    28801 taaaccagga agggtcgcag acgcttacca cctaataatg caccatactg catggtttcg
    28861 accacgggag tgttctgaaa gggcagtggg gcgataaaac ggctcagcgc ctggttggcc
    28921 tgcttaacgc aggcttcgag ttgctgcgga aagtccatta ctcattgtcc ggtgtaaaag
    28981 gggttagaga ggcgtcttca ttgtcagaca gcagaatttg tacgcgctgt tcggcttgtt
    29041 gtaatttggc ctgcccctga cgtgccagct gcacgccgcg ttcgaactcg ttcagcgcct
    29101 cttccagcgg caggtcgcca ctttccagac gggttacaat ctgttccagc tcgctcagcg
    29161 ccttttcaaa gctggcggga gcctcatttt tcttcggcat aatgaatgtc tgactctcaa
    29221 tatttttcgc cccgtcatgg taacggactc agggcaaata gcaaataacg cgcaatggta
    29281 aggtgatgtg cgcagcaaag cgatgttagt ggtatacttc cgcgcctgga tgcagccgca
    29341 ggtgtgggct gctgtatttt tccctataca agtcgcttaa ggcttgccaa cgaaccattg
    29401 ccgccatgaa gtttatcatt aaattgttcc cggaaatcac catcaaaagc caatctgtgc
    29461 gcttgcgctt tataaaaatc cttaccggga acattcgtaa cgttttaaag cactatgatg
    29521 agacgctcgc tgtcgtccgc cactgggata acatcgaagt tcgtgcaaaa gatgaaaacc
    29581 agcgtctggc tattcgcgac gccctgaccc gtattccggg tatccaccat attctcgaag
    29641 tcgaagacgt gccgtttacc gacatgcacg atatttttga gaaagcgttg gttcagtatc
    29701 gcgatcagct ggaaggcaaa accttctgcg tacgcgtgaa gcgccgtggc aaacatgatt
    29761 ttagctcgat tgatgtggaa cgttacgtcg gcggcggttt aaatcagcat attgagtccg
    29821 cgcgcgtgaa gctgaccaat ccggaagtga ctgtccatct ggaagtggaa gacgatcgtc
    29881 tcctgctgat taaaggccgc tacgaaggta ttggcggttt cccgatcggc acccaggaag
    29941 atgtgctgtc gctcatttcc ggtggtttcg actccggtgt ttccagttat atgttgatgc
    30001 gtcgcggctg ccgcgtgcat tactgtttct ttaaccttgg cggcgcggcg catgaaattg
    30061 gcgtgcgtca ggtggcgcat tatctgtgga accgttttgg cagctcccac cgcgtgcgtt
    30121 ttgtcgctat taatttcgaa ccggtcgtcg gggaaattct cgagaaaatc gacgacggtc
    30181 agatgggcgt tatcctcaaa cgtatgatgg tgcgtgccgc gtctaaagtg gctgaacgtt
    30241 acggcgtaca ggcgctggtc accggcgaag cgctcggcca ggtgtccagt cagacgctga
    30301 ccaacctgcg cctgattgat aacgtctccg atacgctgat cctgcgtccg ctgatttctt
    30361 acgacaaaga gcacatcatc aacctggctc gccagattgg caccgaagac tttgctcgca
    30421 cgatgccgga atattgcggt gttatctcca aaagcccgac ggtgaaagcg gttaagtcga
    30481 agattgaagc ggaagaagag aaattcgact tcagcattct cgataaagtg gttgaagaag
    30541 cgaataacgt tgatatccgc gaaatcgccc tgcagaccga gcaggaagtg gtggaagtgg
    30601 aaaccgtcaa tggcttcggc ccgaacgacg tgatcctcga tatccgttct atcgatgaac
    30661 aggaagataa gccactgaaa gtcgaaggga ttgatgtagt ttctctgccg ttctataaac
    30721 tgagcaccaa gtttggcgat ctcgaccaga acagaacctg gctactgtgg tgtgagcgcg
    30781 gggtgatgag ccgtctgcag gcgctctatc tgcgcgagca gggctttaac aatgtgaagg
    30841 tatatcgccc gtaatttgtg gttgtaacgt cgcatctggt cagatgcgac gcttaccgca
    30901 tccgacacta ctcgtaataa ttataaatcc ctgccgccat caccagttgt gatgccactt
    30961 catgggcttt ctcgcgccca acctgcaggt cgataatttt cagaccaaag tcgatagctg
    31021 tccccggccc ctggctggtc agcaatttta cccgtgcatc ccagacgacg cgcttgtcct
    31081 gccattgttc agcgggaatt ttgtctttca gcgtcgggaa gccggtcata ttaccaatcg
    31141 ggaagatatc gtgcggcacc agcacggtgg ctggcgcggc gcaaatagcc gcgacgatac
    31201 gcccggaacg gtggaactgt ttaacggttt caaccagcag agtgctatcg cgaaaacact
    31261 ctgcgccttt aatgccacca ggcagtacga tcacgtcata ttcgccatca gccacctcga
    31321 ccagcggcgc atccgccagc agcttcacgc cgcgcgagca ggtaattgcc aggttgccat
    31381 cgctggctac gctggcagtg gtgactttga taccgccgcg aaccaagagg tcgatcgtgg
    31441 tgacggcttc agtctcttca ctaccagggg cgaggcaaac cagtgccgat gcgctcatat
    31501 tcactctcct ttctttttac catttcaaac aggcgggtgt tttccggtac ggcaatccca
    31561 tgcgcgcggg cgcggcgtaa gagaaaacca ttgatatagt cgatttcggt gtggcgcagc
    31621 gcgcggatat cctgcaacat cgacgagata ttttccgctg tggcatcaat cacctgcatc
    31681 acgtaatcac gcaaatcttc cgctgaagta tgatgccctt cgcgttcgat cactgccgcg
    31741 acttcttcgc atatttgcat aatttcttgc ggatgatgac gtaattcacc gttcgggcaa
    31801 ttccagatgg cggtcagtgg attaatcacg cagttgactg ccagcttgcg ccacagctcg
    31861 gcgcgaatat tgttatgcca ggcaacgtca ggcaacacgg tttgtaaaat atccgccaga
    31921 taactgtaat cgccatcctg ttgccgtgcc ggaccaatat gcgtgatacc gtttgccaca
    31981 tgaataatga cattgccgtc gcggcgggcg gcatgggtgg tggtgcccat cagtaatggc
    32041 tgctgaatat tttgcaactc ttcgatggtg cccatgccgt tgtgaattaa cagtattggc
    32101 gtggttacag gcagtgtgga cgcgaggctt ttgacggcat cggaaacctg ccatgctttc
    32161 agcgtcacca ggagcagatc gctggtggcg agaaaatcgg gatcgttggc ggtcagcgat
    32221 tcgttaaata tcgaaccatc tgtctcaacc agattcgcac tacaataagg ttgcggtacg
    32281 cgcagccagc cctgaacttc atgaccctgt ttgcaaagtg ctgtaagcca taattgccct
    32341 aaggcaccgc atcccaatac ggtaattttc attgttcctc ctcacccgca accactccgg
    32401 gtgttcaata aggctatccc ttaattgtgc atgctgttgc gactatgcac aattaaggga
    32461 tacgtcctgg tgcaggactg tcggttattt aactttgcgg gtattatgct tcgcatcaaa
    32521 aatgaaggga gaggaaaaga tgccatcttt cgatattgtc tctgaagttg atcttcagga
    32581 agcacgtaac gcggtcgata acgcgagccg cgaagtggag tcccgttttg acttccgtaa
    32641 cgttgaagcc tcatttgagc tgaacgacgc cagcaaaacc atcaaagtgt tgagcgagtc
    32701 cgatttccag gtcaatcagt tgctggatat tctgcgtgcc aagctgctga agcgcggcat
    32761 tgaaggcagt tcactggatg taccggaaaa tatcgttcat agcggtaaaa cctggtttgt
    32821 ggaagcgaaa ctgaagcagg gcattgagag cgcgacccag aaaaaaatcg tcaagatgat
    32881 caaagacagc aaactgaaag tgcaggcgca aattcagggc gatgagatcc gcgtgactgg
    32941 caaatctcgt gatgatttgc aggctgtcat ggcgatggtg cgcggtggcg atctgggtca
    33001 gccgttccag ttcaaaaact tccgcgatta atcgcgacaa cctgcgcttt gttcatgtca
    33061 gatgcggcat gaacgcctgg ttcggcctac aaaggcttgt aaatcagggg cttagtaggc
    33121 ctgatgagcg tagcgcagca ggcaatttcg cctcgctttt atgcctggcg aattgcctgt
    33181 tcaacatcaa agcgattcgt cactttgctg tcgattttca cataagctga atgttcttct
    33241 tctgcaatca acacttcttt gatgccttca gtttctagca aacgcacttt taacgcctcg
    33301 tttgcggcaa tgttcgccgg gatttcaatg cgcaaactgc tgacatacgc cggttctttc
    33361 atggtactgg cgactgccag ccacactgcg gccagcattg cgccagcgag aaatacgccc
    33421 tgaccgtcaa acatgccgtc aatccagccg cccagtgaac cgccaatcgc cacgccaaga
    33481 aactggctgg tggagtaaac acccatcgct gtacctttgt aacctgctgg cgactcttta
    33541 ctgataagtg aaggcagaag ggcttccatc aaattaaacg ctacaaagaa aagctgcacg
    33601 ccgaccacca gttgccagaa ctgcgtttgc gcgttccaca acacaatttc cgcaaccacg
    33661 atcaatccga cgcagaagac aaagacttgc ttcattttgc gcttaacttc agcgtagata
    33721 atgaaaggca cgaccgagcc aaaggcgatt agcattgtcg ccagatagac cttccagtgt
    33781 tcagccgccg ggaaccctgc atcagccagt tgtccgggca gggcaacaaa cgtcgacatc
    33841 agcaaaatat gcagacacat aatgccaaag ttgagtttca gcagccgcgg ttccgccagc
    33901 actttactga aactgccttt caccattccg gactcacgat taagtacgtg agtgctactg
    33961 ttgggcacaa cccaaatggt caacgcaatg ccggtcgttg ccagaatagc gatcatccag
    34021 aacagcgcgt gcagcccaag tttgtgagtg atgatcgggc caagcaccat cgcaatggca
    34081 aaggtaatgc caaagctcac gccgataaac gccattgctt tggtgcggtt ttgttcgcgc
    34141 gtgagatcgg aaagcagcgc cataacggca gcggcaatcg caccggagcc ttgtagcgcc
    34201 cggcctagaa taattcccca gatggagtca gagagcgcag cgataacgct accggcggca
    34261 aacaccgcca gcccaccgac aattaatggt ttgcgaccaa tacggtcgga aagcaggcca
    34321 aacggaatct gaaaaacggc ctgagtcaga ccataaatac caatggcaat accgattaat
    34381 gcttcgctgg caccttgcag agccatgcca tacgtggtca gaaccggcag aaccataaac
    34441 atgcccagca tgcgcaacga gaatacggtc cctaaacccc aggtcgcgcg cctctcacct
    34501 ggcgtcattt tataatcgtt cattaccacc tctgttttaa attcgcgact agtgtaaagc
    34561 ggcaaacgaa tggggtaaac atgcggattt tttagtaaat atttcgagag ggatatgttt
    34621 ctaatgctaa gaaaaaaggt gccgtagcac ctttttaata gagaggtttt gttaccacac
    34681 agcagccagc agcgtatgcg agtccggtac cataaaatca acggacatca tcaccgagag
    34741 ggcagtgatg gcgatgatag agaagccgaa cagcttgcgc gcccagattc tgtcatcagc
    34801 aactttataa ccgcgcagag ccatacctaa ccaccagacg ctaaccgccg cggcgaccac
    34861 cagatattta tatccagcgt aaccgccaag agagagcatc agcgtggcaa cggcaaaggc
    34921 gatgatatac agcgtgatgt gattcttcgc caccgaaatg ccttttacca ctggcaatac
    34981 cggaatgttt gccgcctggt aatccttaaa gcggaaaatg gcgatggcat aggagtgagg
    35041 catctgccac aggctgaaga tagccagcag gatcgctgcg ccgctatcga actcgccggt
    35101 tactgcacag tagccaatca ccggcggcgc agcgccggag agcgaaccaa tcaatgtgcc
    35161 gtagactgag tggcgtttca tgtacaggct atagacgccg acataaacca caaagcccat
    35221 cacccccagc cagcaggcca gcggattcgc gccaaaccac agcagcataa agccagcaat
    35281 acccagcaag gtggcgtaca ccagcgagac agcaggagag atcaggcctt taaccagcac
    35341 ccgattcttc gtcctttcca tctttctgtc gatatccctg tcgatgtagt tgttaaacac
    35401 acaacccgac gccacaacca gtgacacccc aaccagcgtg tagataaaca ggggataatc
    35461 aatgctgccc tttgaggcca gcaggaatcc cccaatcacc gagatcaggt tgccaaagat
    35521 gatgcctggt ttcgttactt gcaggtattg cttaaacatc ataaccgccg ctcttagtgc
    35581 atcatcatgt tgtagttgag gttccacata atccagatgg agcctacaac caggatagcg
    35641 atgattagca cggtgaagac aaacgccgtc atgttccagc cttcatctga tttggtattc
    35701 atgtgcagga agcacaccag atgcaccaga acctgtacca ctgccattgc caggattgtt
    35761 cccagaatta cggccggaga ggcagcccct gtcatcacca tccagaacgg aatcaccgtc
    35821 agaatgatcg acaggataaa gcctgtcatg taggttttta cgctgccatg ggacgcgccg
    35881 ctgtgatcgg tagaatgact cattacatcg cccccatcag ataaacaaca gtgaacacac
    35941 agatccaaac cacatccagg aagtgccaga acaggctcag gcacatgatg cgggtacggt
    36001 tagtgctggt caggccgcga cgagcgattt gtaccatcag caccgccatc cagataagac
    36061 cggaagtgac gtgcagaccg tgcgtgccga ccagcgcaaa gaacgctgac aggaagccgc
    36121 tgcgatccgg acccatgccg ttaacaatca ggtgatggaa ttcgtagatt tccatcccga
    36181 taaatccggc accaaacaaa caggtcaacg ccagccagga gataacctgg cttttgttgt
    36241 ttttgtacat ggcgatagcc gccatgccgt aggtgatgga gctgaacaac agcaagaaag
    36301 tttcaaccag aacgaacggc agttcgaaaa tgtccttacc tgtcgggccg cctgcggtgc
    36361 cgttcaccag aacggcatag gtagcaaaca agattgagaa cagaatgcag tcgctcatca
    36421 ggtagatcca aaatccgaag attttggttc cgcctgcatc gtggtgcccg tgttcgtgcg
    36481 cgtgggcagt cgcgtgcgtc aaagtatcag ttgccatttt tcagccctgc cttagtaatc
    36541 tcatcgaaat gctggttttc cagtttttcg atttctgcca ccggcacgta gtaatccacg
    36601 tcctcgtcga agcttttcac gatccaggtg atgatcatgc ctgcgaagcc aacaatcgcc
    36661 agccaccaga tatgccagat catggcgaaa ccgaagatgg tggagaaagc tgcaatgacg
    36721 atacctgcac cgctgttttt cggcatatga atttcttcat agtggtcagg ctttttatac
    36781 gcttcgcctt tctctttcat ttcccagaat gcatcacgct cgtgaacgtg cggcacaacg
    36841 gcaaagttat agaacggagg cggggaagag tttgcccact ccagcgtacg gccaccccac
    36901 gggtcgccag tcaggtcacg gttctggtcg cggtcgcgaa tagaaacgta catctgaata
    36961 acgaggcaga gaatacccag cgcaatcagt actgcaccgc tagctgcaat catcagcatg
    37021 gtgtggaact gcgggtcaat ctgctggctc aaacgacggg tcatgcccat gaagcccagt
    37081 gcatacagcg gcataaaggc aacgaagaag ccgatgatcc agaaccagaa cgcgcgttta
    37141 ccccaggttt cattcagttt gaaaccgaac gctttaggcc accagtaggt catccctgcg
    37201 aagcagccga agaccacgcc gccgatgata acgttatgga agtgcgcaat caggaacagg
    37261 ctgttatgca gaacgaagtc cgcgcccggt actgccagca gcacgccagt catcccgccc
    37321 accgagaaag tgacgataaa accgatggtc cacagcatcg cagaatggaa cacgatacgg
    37381 ccctgataca tggtgaacag ccagttgaag atcttcaccc cggtcgggat ggcgataatc
    37441 attgtggtga taccaaagaa ggcgtttacg ttcgcgcccg cacccatcgt aaagaagtgg
    37501 tgcagccaaa cgatgaacga cagcacggtg atacagacgg ttgcccatac cagcgaggta
    37561 taaccaaaca gacgtttacg cgagaaggtt gccgcaattt cggagaacac accgaaaaca
    37621 ggcaggatca ggatgtaaac ttccgggtgg ccccaggccc aaatcaggtt gatgtacatc
    37681 atcatgttgc cacccatatc gttggtaaag aaatgggtgc ccagatagcg atccagggtc
    37741 aacaacgcga cggtaaccgt cagaattggg aaggaagcaa taatcagtac gttcgcgcac
    37801 agtgatgccc aggtaaatac tggcatcttg aacatggtca tgcccggtgc gcgcatcttc
    37861 agaatggtaa cgaagaagtt gataccggta agcgtcgtac ctataccgga tagctggaga
    37921 ctccatatcc agtaatcaac accgactccc ggactgtact ctattcccga tagcggtgga
    37981 taggccagcc agccggtctg cgcaaattcg cccacgccga gagaaacgtt aaccagaatc
    38041 acaccaacaa cagtaaacca gaagcttaag ttgttgagga acgggaacgc aacgtcacgc
    38101 gcgccgatct gcagcggaac caccaggttc atcagaccga taacgaaagg catcgccacg
    38161 aagaagatca taatcacgcc gtgcgcggtg aagatctgat cgtagtggtg aggtggcagg
    38221 aagcccgctt cgcccgccga ggcaagagcc tgctggctac gcatcataat ggcgtcagca
    38281 aaaccacgca gcaacatcac aatcgccacg atgatataca tgataccgag gcgtttatgg
    38341 tcgacggagg tcagccactc tttccacagg taggtccact taccgaagta agtgatcagg
    38401 ccaacgagcg ccagacctcc caaaataatg ccagcgatcg taaccatgac gataggttca
    38461 tggaacggga ctgcatcaag tgataatttt ccgaacatct ttattcttcc tcaacccctt
    38521 taatgggcgg attccgcgtg gctcatgtcc atgccttcca taccttcgtg tgcgctgtgc
    38581 tcaccttctg gctgggtcat gtccatgctc ttaccgtgag ccataaactt gttaatcaca
    38641 tcagcaaaca agtctggttt cacgttggag aaatattcca cctggttgta ttcgctaggc
    38701 gcggccagtt tttcgaacgc agccatgtca gacatggtgt tcggcgactg cttcgctttt
    38761 gcgacccact ggtcgaattc ggcgcgatcc ggtgttgcaa tagctttgaa cttcatgcct
    38821 gagaagcccg ggccgctgta gctggcggag ataccgtcat aagtaccggg ttcgttggcg
    38881 atcagatgca ggcgagtctg cataccagcc atggcataaa tctggctacc cagacgcgga
    38941 atgaagaagg agttcatcac ggagttggag gtcactttga agtacaccgg agtgttcgcc
    39001 gggaaagcga tttcattcac ggtagcaatg ccctgttccg ggtagatgaa gaaccatttc
    39061 cagtccatgg aaaccacttc gatggtaatg ggcttctcgt cgtgtgccag cggcttgcta
    39121 ggctcaagag cgtgagtggt tttccaggtc agtaccgcaa ggaagatgat gattaagata
    39181 ggtaccgtcc agaccacagc ttccacttta ttggagtgtg accagttcgg gctgtactta
    39241 gcatctttat tgctcgcacg atacttccag gcgaaaccaa cagccatcaa gattgcggga
    39301 ataacgacaa tcaacatcag gccaaatgcc gtcagtatca gtgaacgttg ctccagacca
    39361 atctgtcctt tgggatctaa cagcgcagaa ttacagccac tgagcaatac agtgcctgca
    39421 aataatgaca accatcccaa acttttattg tatttcctga gtctcattta acgacctcaa
    39481 ttccacggga tctggtggcg tttaaagtgt gtgggcattt tatgggaagg ttacattact
    39541 gtaaacatga ttaaattcct gttacttgga tttgactgtc aggtcacata tgttgcaaaa
    39601 catatcaagt tgattttaag aagctgttgc aaaagggcgt tataacgaaa ggggagtgca
    39661 gaaggagttt ggatgggcaa ttggtataac caatctggaa taaaataatt gtttaacaaa
    39721 taattatcat tatggtgaca attgggtggg gggaaaataa agactcgtta gctgaatcgt
    39781 gtaagaaaaa atagaattca taaattgcgt caataatttc caaacgctaa gccgcacaaa
    39841 agaacaaata ttattaagcg atactgatgt ttatttagcc gtaataatta cggcgagtga
    39901 ttactacagc taaataatat ttacagatta cgtcagatgc gtttttcgta gagccagata
    39961 atccagcaaa ccaccaagca cgacgccaga aagggcgact aaaaccccga cttccagcag
    40021 agcaggcagg aaagagaagt gcgtcaaatc cagcgcgtcc atcgtcaaca gcagtaacca
    40081 cacggccaac aggctgacgc ccgccgccag tgtccacatg gcaaaggcat aacctgccgg
    40141 atattcggta cgggagataa agttgtcatt tactcgtgta tattcaagcg tctggcggca
    40201 aaccagcagc aaaataagcc ccggtacggc agcggcgacg gagaatagat agaatgtcga
    40261 ccagccgtgt gcttcaacaa accaacccgc cacggggccg acataaactc gtcctacagc
    40321 ggaaagcgct gagagcaggg caaattgagt agcggaaaat gacttattac acagcgtcat
    40381 taacagcgcg acaaaggctg atgtgcccat cccgccacag aggttttcga aaaagacggc
    40441 tgcgcccatg ctgtagagat gcttatcagt aatcgacagc agccagtaac cagcgttaga
    40501 cgcaccttgt aaaatgccga aaatcagcag cgcccggaac agcgacaggc gctgcatcaa
    40561 aatcccaccg tacaatgcgc ccacaatggt cgccagtaag ccaagcgttt tgttaaccac
    40621 gcctacttca cccgcatcaa acccgacgcc gcgaatcaaa aacgtggttg tcaggctcat
    40681 ggcgaatgcg tcgcccagct tatacagcac gataagaagc aaaataagcc aggcattatt
    40741 gcgaccaaag aaatctcgca gaggtgcaac aaccgcttgt tccagcgttt ttggcacagg
    40801 aatggtgtcg gtcggttctg gtgcaagcaa cgtcgcgata atacaaggga tcaacagcgc
    40861 cgccatcaac cagtacatgc cctgccagcc cagccattta tctgccagcc acagggctaa
    40921 gccgccggaa accagcatcc ctaaacggta acccagcacg ctgattgccg cgcccgcacc
    40981 acgttcttct gccgggagca catcggtttt ccacgcatcg aagacaatat cctgggaggc
    41041 cgagcaaaaa gcgatcacca ccgccagtgc cgccatccag cggagttggg tgccaggttc
    41101 gagaaaaccc atcgccgcaa tggcgactaa taacaggatt tgcgtggcga gcagccaacc
    41161 gcgccgccgc ccaaaaaatg ggggcgtgta gcggtccatc agcggtgacc agaggaattt
    41221 aaaaacgtaa gcctggccta ccagagagaa gaaaccaatg gttttgagat caatattctc
    41281 gaccgtcatc caggcctgta aggtaccgga tgtcagggcg agcggtagcc cggaagcaaa
    41341 acccaggatc agcaatatgg ctgaacgcgg ctgttgaaaa atacgtaaat attgactgga
    41401 catgggcgtc tacaggcccg gtttgcaccg ggccagaggg cagaaattaa cgcgcgttct
    41461 gcttgatgaa ttcgtgaatg ctggtgtcct gagacatatc agcgatggta tcggtcagca
    41521 cactgttaac cgcatcggcg atatttttgt tggaggcctg gaacgcacct tcaacgttgt
    41581 agctggcacg atagtttttg gttattttgt tgccattctg cgcggtagcg atgatggcga
    41641 tatccgcttt ggtagcgatg ttgtagcgca cgttgccctg ggacacgtca gcatacagtt
    41701 ggctaacgat gatttgcaga ttaaccgggc cattcggacc aaccatgtaa ccacgcgcgg
    41761 tcatctgttt ttccagcact tcttgcagca ggaaacgcag atcgcgggag gcggccaggg
    41821 taacgatttg attatcgcgg gtgacttttg ccagcgcctg atcggtacgc tgatcggcac
    41881 cattaatgct tacggtgacg cccatcaggc ttggatcctg ctgtggcagt gtaatcgtcg
    41941 gggaaacttc aatagttgtt ggcggttttg cgcatcctgc aagcataaac agagcaacta
    42001 acgggaagag gatttttttg aacatgttcg ggctctcaga gactcttaag cgtgtttggt
    42061 aaaaattccc gccatcataa cattgccaac ggcgagggga agtgggtaag gcatgtaaat
    42121 tcatcatgtt gacgaaataa tcacccctgg taaaagaaac gctgatgcga ggcctgtgtt
    42181 tcaatcttta aatcagtaaa cttcatacgc ttgacggaaa aaccaggacg aaacctaaat
    42241 atttgttgtt aagctgcaat ggaaacggta aaagcggcta gtatttaaag ggatggatga
    42301 catctcagcg ttgtcggagg agatatttca tgatgatacg tgagcggata gaagaaaaat
    42361 taagggcggc gttccaaccc gtattcctcg aagtagtgga tgaaagctat cgtcacaatg
    42421 tcccagccgg ctctgaaagc cattttaaag ttgtgctggt cagcgatcgt tttacgggtg
    42481 aacgttttct gaatcgtcat cgaatgattt acagtacttt agcggaggaa ctctctacta
    42541 ccgttcatgc gctggctctg catacttaca ctattaagga gtgggaaggg ttgcaggaca
    42601 ccgtctttgc ctctcctccc tgtcgtggag caggaagcat cgcgtaaaaa cgcatttgca
    42661 actgtcggcg ctttttcagt atgttgctaa agattttatg aaaaacggcc tgcgggccgt
    42721 tttgttttgt ctggattttg cgctttttgc ccagcattca gacgaaaatt gcccgggaat
    42781 tgtgaaaaaa tacgcgacag cgcgcaataa ccgttctcga ctcataaaag tgatgccgct
    42841 ataatgccgc gtcctatttg aatgctttcg ggatgattct ggtaacaggg aatgtgattg
    42901 attataagaa catcccggtt ccgcgaagcc aacaacctgt gcttgcgggg taagagttga
    42961 ccgagcactg tgattttttg aggtaacaag atgcaagttt cagttgaaac cactcaaggc
    43021 cttggccgcc gtgtaacgat tactatcgct gctgacagca tcgagaccgc tgttaaaagc
    43081 gagctggtca acgttgcgaa aaaagtacgt attgacggct tccgcaaagg caaagtgcca
    43141 atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc aggacgttct gggtgacctg
    43201 atgagccgta acttcattga cgccatcatt aaagaaaaaa tcaatccggc tggcgcaccg
    43261 acttatgttc cgggcgaata caagctgggt gaagacttca cttactctgt agagtttgaa
    43321 gtttatccgg aagttgaact gcagggtctg gaagcgatcg aagttgaaaa accgatcgtt
    43381 gaagtgaccg acgctgacgt tgacggcatg ctggatactc tgcgtaaaca gcaggcgacc
    43441 tggaaagaaa aagacagcgc tgttgaagca gaagaccgcg taaccatcga cttcaccggt
    43501 tctgtagaca gcgaagagtt cgaaggcggt aaagcgtctg atttcgtact ggcgatgggc
    43561 cagggtcgta tgatcccggg ctttgaagac ggtatcaaag gccacaaagc tggcgaagag
    43621 ttcaccatcg acgtgacctt cccggaagaa taccacgcag aaaacctgaa aggtaaagca
    43681 gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg aactgccgga actgactgca
    43741 gaattcatca aacgtttcgg cgttgaagat ggttccgtag aaggtctgcg cgctgaagtg
    43801 cgtaaaaaca tggagcgcga gctgaagagc gccatccgta accgcgttaa gtctcaggcg
    43861 atcgaaggtc tggtaaaagc taacgacatc gacgtaccgg ctgcgctgat cgacagcgaa
    43921 atcgacgttc tgcgtcgcca ggctgcacag cgtttcggtg gcaacgaaaa acaagctctg
    43981 gaactgccgc gcgaactgtt cgaagaacag gctaaacgcc gcgtagttgt tggcctgctg
    44041 ctgggcgaag ttatccgcac caacgagctg aaagctgacg aagagcgcgt gaaaggcctg
    44101 atcgaagaga tggcttctgc gtacgaagat ccgaaagaag ttatcgagtt ctacagcaaa
    44161 aacaaagaac tgatggacaa catgcgcaat gttgctctgg aagaacaggc tgttgaagct
    44221 gtactggcga aagcgaaagt gactgaaaaa gaaaccactt tcaacgagct gatgaaccag
    44281 caggcgtaat ttacgcagca taacgcgcta aattcgcaca aaggcccgtc accgccaggt
    44341 ggtgggcttt ttttgtcatg aattttgcat ggaaccgtgc gaaaagcctc tttcggtgtt
    44401 agcgtaacaa caaaagattg ttatgcttga aatatggtga tgccgtaccc ataacacagg
    44461 gactagctga taatccgtcc ataaggttac aatcggtaca gcaggttttt tcaattttat
    44521 ccaggagacg gaaatgtcat acagcggcga acgagataac tttgcacccc atatggcgct
    44581 ggtgccgatg gtcattgaac agacctcacg cggtgagcgc tcttttgata tctattctcg
    44641 tctacttaag gaacgcgtca tttttctgac tggccaggtt gaagaccaca tggctaacct
    44701 gattgtggcg cagatgctgt tcctggaagc ggaaaaccca gaaaaagata tctatctgta
    44761 cattaactcc ccaggcgggg tgatcactgc cgggatgtct atctatgaca ccatgcagtt
    44821 tatcaagcct gatgtcagca ccatctgtat gggccaggcg gcctcgatgg gcgctttctt
    44881 gctgaccgca ggggcaaaag gtaaacgttt ttgcctgccg aattcgcgcg tgatgattca
    44941 ccaaccgttg ggcggctacc agggccaggc gaccgatatc gaaattcatg cccgtgaaat
    45001 tctgaaagtt aaagggcgca tgaatgaact tatggcgctt catacgggtc aatcattaga
    45061 acagattgaa cgtgataccg agcgcgatcg cttcctttcc gcccctgaag cggtggaata
    45121 cggtctggtc gattcgattc tgacccatcg taattgatgc cagaggcgca actgtgccgc
    45181 tatacttatc cagggcggca caacgctgta agcggcttgc gcctgagaat ggcatttgcg
    45241 tcgtcgtgtg cggcacaaag aacaaagaag aggttttgac ccatgacaga taaacgcaaa
    45301 gatggctcag gcaaattgct gtattgctct ttttgcggca aaagccagca tgaagtgcgc
    45361 aagctgattg ccggtccatc cgtgtatatc tgcgacgaat gtgttgattt atgtaacgac
    45421 atcattcgcg aagagattaa agaagttgca ccgcatcgtg aacgcagtgc gctaccgacg
    45481 ccgcatgaaa ttcgcaacca cctggacgat tacgttatcg gccaggaaca ggcgaaaaaa
    45541 gtgctggcgg tcgcggtata caaccattac aaacgtctgc gcaacggcga taccagcaat
    45601 ggtgtcgagt tgggcaaaag taacattctg ctgatcggtc cgaccggttc cggtaaaacg
    45661 ctgctggctg aaacgctggc gcgcctgctg gatgttccgt tcaccatggc cgacgcgact
    45721 acactgaccg aagccggtta tgtgggtgaa gacgttgaaa acatcattca gaagctgttg
    45781 cagaaatgcg actacgatgt ccagaaagca cagcgtggta ttgtctacat cgatgaaatc
    45841 gacaagattt ctcgtaagtc ggacaacccg tctattaccc gtgacgtttc cggtgaaggc
    45901 gtacagcagg cactgttgaa actgatcgaa ggtactgttg ctgccgttcc accgcagggc
    45961 gggcgtaagc atccgcagca ggaatttttg caggttgata cctccaagat cctgtttatt
    46021 tgtggcggtg cgtttgccgg tctggataaa gtgatttccc accgtgtaga aaccggctcc
    46081 ggcattggtt ttggcgcgac ggtaaaagcg aagtccgaca aagcaagcga aggcgagctg
    46141 ctggcgcagg ttgaaccgga agatctgatc aagtttggtc ttatccctga gtttattggt
    46201 cgtctgccgg ttgtcgcaac gttgaatgaa ctgagcgaag aagctctgat tcagatcctc
    46261 aaagagccga aaaacgccct gaccaagcag tatcaggcgc tgtttaatct ggaaggcgtg
    46321 gatctggaat tccgtgacga ggcgctggat gctatcgcta agaaagcgat ggcgcgtaaa
    46381 accggtgccc gtggcctgcg ttccatcgta gaagccgcac tgctcgatac catgtacgat
    46441 ctgccgtcca tggaagatgt cgaaaaagtg gttatcgacg agtcggtaat tgatggtcaa
    46501 agcaaaccgt tgctgattta tggtaagccg gaagcgcaac aggcatctgg tgaataatta
    46561 accattccca tacaattagt taaccaaaaa ggggggattt tatctcccct ttaatttttc
    46621 ctctattctc ggcgttgaat gtgggggaaa catccccata tactgacgta catgttaata
    46681 gatggcgtga agcacagtcg tgtcatctga ttacctggcg gaaattaaac taagagagag
    46741 ctctatgaat cctgagcgtt ctgaacgcat tgaaatcccc gtattgccgc tgcgcgatgt
    46801 ggtggtttat ccgcacatgg tcattccctt atttgtcggg cgggaaaaat ctatccgttg
    46861 tctggaagcg gcgatggacc atgataaaaa aattatgctg gtcgcgcaga aagaagcttc
    46921 aacggatgag ccgggtgtaa acgatctttt caccgtcggg accgtggcct ctatattgca
    46981 gatgctgaaa ctgcctgacg gcaccgtcaa agtgctggtc gaggggttac agcgcgcgcg
    47041 tatttctgcg ctctctgaca atggcgaaca cttttctgcg aaggcggagt atctggagtc
    47101 gccgaccatt gatgagcggg aacaggaagt gctggtgcgt actgcaatca gccagttcga
    47161 aggctacatc aagctgaaca aaaaaatccc accagaagtg ctgacgtcgc tgaatagcat
    47221 cgacgatccg gcgcgtctgg cggataccat tgctgcacat atgccactga aactggctga
    47281 caaacagtcc gttctggaga tgtccgacgt taacgaacgt ctggaatatc tgatggcaat
    47341 gatggaatcg gaaatcgatc tgctgcaggt tgagaaacgc attcgcaacc gcgttaaaaa
    47401 gcagatggag aaatcccagc gtgagtacta tctgaacgag caaatgaaag ctattcagaa
    47461 agaactcggt gaaatggacg acgcgccgga cgaaaacgaa gccctgaagc gcaaaatcga
    47521 cgcggcgaag atgccgaaag aggcaaaaga gaaagcggaa gcagagttgc agaagctgaa
    47581 aatgatgtct ccgatgtcgg cagaagcgac cgtagtgcgt ggttatatcg actggatggt
    47641 acaggtaccg tggaatgcgc gcagcaaggt caaaaaagac ctgcgtcagg cgcaggaaat
    47701 ccttgatacc gaccattatg gtctggagcg cgtgaaagat cgcatccttg agtaccttgc
    47761 ggttcaaagc cgtgtcaaca aaatcaaggg accgatcctc tgcctggtag ggccgccggg
    47821 ggtaggtaaa acctctcttg gtcagtccat tgccaaagcc accgggcgta aatatgtccg
    47881 tatggcgctg ggcggcgtgc gtgatgaagc ggaaatccgt ggtcaccgcc gtacttacat
    47941 cggttctatg ccgggtaaac tgatccagaa aatggcgaaa gtgggcgtga aaaacccgct
    48001 gttcctgctc gatgagatcg acaaaatgtc ttctgacatg cgaggcgatc cggcctctgc
    48061 actgcttgaa gtgctggatc cagagcagaa cgtagcgttc agcgaccact acctggaagt
    48121 ggattacgat ctcagcgacg tgatgtttgt cgcgacgtcg aactccatga acattccggc
    48181 accgctgctg gatcgtatgg aagtgattcg cctctccggt tataccgaag atgaaaaact
    48241 gaacatcgcc aaacgtcacc tgctgccgaa gcagattgaa cgtaatgcac tgaaaaaagg
    48301 tgagctgacc gtcgacgata gcgccattat cggcattatt cgttactaca cccgtgaggc
    48361 gggcgtgcgt ggtctggagc gtgaaatctc caaactgtgc cgcaaagcgg ttaagcagtt
    48421 actgctcgat aagtcattaa aacatatcga aattaacggc gataacctgc atgactacct
    48481 cggtgttcag cgtttcgact atggtcgcgc tgataacgaa aactgtgtcg gtcaggtaac
    48541 cggtctggcg tggacggaag tgggcggtga cttgctgacc attgaaaccg catgtgttcc
    48601 gggtaaaggc aaactgacct ataccggttc gctcggcgaa gtgatgcagg agtctattca
    48661 ggcggcgtta acggtggttc gtgcgcgtgc ggaaaaactg gggatcaacc ctgattttta
    48721 cgaaaaacgt gacatccacg tccacgtacc ggaaggtgcg acgccgaaag atggtccgag
    48781 tgccggtatt gctatgtgca ccgcgctggt ttcttgcctg accggtaacc cggttcgtgc
    48841 cgatgtggca atgaccggtg agatcactct gcgtggtcag gtactgccga tcggtggttt
    48901 gaaagaaaaa ctcctggcag cgcatcgcgg cgggattaaa acagtgctaa ttccgttcga
    48961 aaataaacgc gatctggaag agattcctga caacgtaatt gccgatctgg acattcatcc
    49021 tgtgaagcgc attgaggaag ttctgactct ggcgctgcaa aatgaaccgt ctggcatgca
    49081 ggttgtgact gcaaaatagt gacctcgcgc aaaatgcact aataaaaaca gggctggcag
    49141 gctaattcgg gcttgccagc ctttttttgt ctcgctaagt tagatggcgg atcgggcttg
    49201 cccttattaa ggggtgttgt aaggggatgg ctggcctgat ataactgctg cgcgttcgta
    49261 ccttgaagga ttcaagtgcg atataaatta taaagaggaa gagaagagtg aataaatctc
    49321 aattgatcga caagattgct gcaggggctg atatctctaa agctgcggct ggccgtgcgt
    49381 tagatgctat tattgcttcc gtaactgaat ctctgaaaga aggggatgat gtagcactgg
    49441 taggttttgg tacttttgcc gttaaagagc gtgctgcccg tactggccgc aacccgcaga
    49501 ccggtaaaga gatcaccatc gctgctgcta aagtaccgag cttccgtgca ggtaaagcac
    49561 tgaaagacgc ggtaaactaa gcgttgtccc cggtggggat gtgacgaagt tcaagggcgc
    49621 atctactgat gtgccttttt tatttgtatt cggtgacttt ctgcgtcttg tgggctgaca
    49681 attgcccccg tttcttgtca caataggcct ttgcgcgcat cgatacgttg cgtgaggtac
    49741 acagtcatct acagcggagt gttgttacac catgatggac agcttacgca cggctgcaaa
    49801 cagtctcgtg ctcaagatta ttttcggtat cattatcgtg tcgttcatat tgacaggcgt
    49861 gagtggttac ctgattggcg gaggcaataa ctacgccgca aaagtgaatg accaggaaat
    49921 cagccgtggg cagttcgaga acgcctttaa cagcgagcgt aatcgcatgc agcaacagct
    49981 gggcgatcaa tactccgagc tggcagcgaa tgaaggctat atgaaaaccc tgcgtcaaca
    50041 ggtgctgaat cgtctgatcg acgaggcgct gttggatcag tacgctcgtg agctgaaact
    50101 gggtatcagt gatgagcagg ttaaacaggc gattttcgcg accccagcct tccaggttga
    50161 cggcaaattt gataacagcc gctataacgg tatcctcaac cagatgggga tgaccgccga
    50221 tcagtacgcc caggcgctgc gtaaccagct cactacccaa cagctgatta acggtgttgc
    50281 cggtaccgat tttatgctga aaggtgaaac cgacgagctg gcggcactgg tcgcgcaaca
    50341 acgcgtggtg cgtgaggcga ctatcgatgt taacgcgctg gcggcgaagc agcctgtgac
    50401 cgaacaggag attgccagct actacgaaca aaacaaaaac aatttcatga cgccggaaca
    50461 attccgcgtg agttacatca agctggatgc cgcaacgatg cagcaaccgg ttagcgatgc
    50521 ggatatccag agctactacg atcagcatca ggatcaattc acccagccgc agcgtacccg
    50581 ctacagcatc atccagacca aaactgaaga tgaagcgaaa gcggtacttg atgagctgaa
    50641 taaaggcggt gattttgctg cattagccaa agaaaaatct gccgatatta tctctgcccg
    50701 taacggcggc gatatgggtt ggttagaaga tgccactatc cccgacgaac tgaaaaatgc
    50761 tggtctgaaa gaaaaaggcc aactgtctgg tgtcatcaaa tcttcggtcg gtttcctgat
    50821 tgtacgtctg gacgacattc agccagcgaa agtgaaatcg ttagacgaag tacgtgacga
    50881 cattgcggcg aaagtgaaac acgaaaaagc cctcgatgcg tactatgcgc tgcagcagaa
    50941 agtgagcgat gcggcaagca acgacaccga gtctctggcc ggtgcagagc aagctgccgg
    51001 cgttaaagcc actcagacgg gttggttcag caaagataac ctgccggaag agttgaactt
    51061 caagccggtt gccgacgcta tctttaacgg cggtctggta ggtgaaaacg gcgcgccggg
    51121 catcaactct gacatcatca ccgtagacgg cgaccgtgca ttcgtgctgc gcatcagcga
    51181 gcacaaaccg gaagcggtga aaccgttggc agatgttcag gaacaagtta aggcactggt
    51241 tcagcacaac aaagctgaac aacaggcgaa agtggatgct gagaaactgc tggttgattt
    51301 gaaagccggc aaaggtgcgg aagctatgca ggctgccggt ctgaaatttg gcgagccgaa
    51361 aaccttaagc cgttccggtc gtgacccgat tagccaggcg gcgtttgcac tgccactgcc
    51421 agcgaaagac aaaccgagct acggtatggc gaccgatatg caaggtaatg tggttctgct
    51481 ggcgctggat gaagtgaaac aaggttcaat gccggaagat cagaaaaaag cgatggtgca
    51541 gggtatcacc cagaacaacg cacaaatcgt ctttgaagct ctgatgagta acctgcgtaa
    51601 agaggcgaaa atcaaaattg gcgatgcgct ggaacagcaa taatcctgaa gccgcctcgc
    51661 aaaaaaatgc gttgcagctg taacaagaaa aggtcgcttt cgcggccttt tccatttctg
    51721 aacattgcca tttgtttact gttttcactg ccgttaaggt gattccactg ttaacaaaca
    51781 aggagaaaac agtatgaaac acggaattaa agcactgctc attaccctgt ccctggcctg
    51841 tgccggaatg tctcatagcg cgctggcggc agcttctgtg gcgaaaccga cggcggtaga
    51901 aaccaaagcg gaagctcctg cagcacaaag taaagcagca gtaccggcga aagccagtga
    51961 cgaagaaggc acccgggtca gcattaataa tgccagcgcg gaagagctag cccgcgcgat
    52021 gaatggcgtt ggcctgaaga aagcgcaggc gattgtcagt tatcgcgaag agtacggtcc
    52081 gtttaaaaca gtggaggatc taaagcaggt gccggggatg ggcaattcgc tggtggaacg
    52141 taatctggcg gtattaaccc tgtaattaat ttgcatagtg gcaatttttt gccagactga
    52201 agaggtcata ccagttatga cctctgtact tataacaaca acgtaaggtt attgcgctat
    52261 gcaaacacaa ataaaagttc gtggatatca tctcgacgtt taccagcacg tcaacaacgc
    52321 ccgctacctt gaatttctcg aagaagcccg ctgggatggg ttggaaaata gcgacagttt
    52381 tcagtggatg acggcccata acatcgcctt cgtcgtggtc aatatcaata ttaactatcg
    52441 tcgcccagcg gtattaagtg acctgttaac tattaccagt cagttgcagc aattaaacgg
    52501 taaaagcggc atcttaagcc aggtcattac actggagccg gaagggcagg tggtagcgga
    52561 tgcgcttatt acgtttgttt gtattgatct taaaacgcag aaagcattag ctctggaagg
    52621 ggaattgcgc gaaaagctgg agcagatggt taagtaaacg ttttgtggtg ccggatgctc
    52681 aagccgcatc cggcgacacc cggaataatt acctcaaccc ggttttctgc ttcatcgctg
    52741 ccatcaccgt cggtttatcg gccagataat gattcaaacc gttggcgcgt aaattacatg
    52801 ccgcacaatg accgcaaccg tcgcctttaa tgccgttata gcaggtcaac gtttcgttac
    52861 ggactaaatc cagtttgccg taataatctg ccagcgccca ggtttccgct ttatcaatcc
    52921 acatcagcgg cgtttcaaaa cgaatatctt tcgccatgcc caaactgacg gcatggttta
    52981 gtgctttcac aaactcatcg cggcaatccg ggtagccgga gaaatccgtt tcacagacgc
    53041 cggtaattac ggcttctgct tttacctgat acgcatatat tgccgccagc gtcaggaaca
    53101 aaatattacg ccctgggaca aacgtattcg ggatgccatc ggcttcaggt tcataatcag
    53161 gcaccggaat gctgtcacgt gtcaggctgc tgaccgccag ctcgttgagc agcgttacat
    53221 caagaacctt atgcgcgcgt gctcccagtt tcagcgccag ttcgcgtgcc acgtcgattt
    53281 ctgcgcgatg tcgctgaccg taatcgaacg tcacgcaatg gacttcatca tattgttgta
    53341 atgcctgcac cagacaggtg gtggaatcct ggcctccact gaacacaacg acagcacgtt
    53401 tcatagataa tcctgcttga acaataaaag cgttatggta acgcctgcga ttaacccgga
    53461 ccagctattc attgcgacgg cgcgggaagc caggcttcgg taaattcaaa ccagccgcgc
    53521 ggtgtcagtc gcacaccgtt cacgccggga ggggcactaa tgcgatagtg atagttgaac
    53581 agcggcgtca gcgtcgcgtc tgccattaac tggctaaaaa ccgctttcag ggcattaaat
    53641 cggttttcct catcaggcat tacttgcacc gcgtccagcg tcgattgcag atgtgcatat
    53701 gctggagcgt cgaaaacatg tggccatagt ggatcgcaac gtagccattg ctccagagta
    53761 tattccggtg cttcgccaat taatctgtcg cccatcatga ggtctgcgtg tgccagtagg
    53821 gtcgtgtcgt cccagttttt tgcgttatga aaaataattg tgagttcaca gccttccgct
    53881 gccagtgtcg cctgtaggcg ttctgccatg gtatgaagtt ctatcggtag gtgataaacc
    53941 agcgtcaagg ttttcggtag tttgacttca tccggtactt gccagtgcgg aatagtccag
    54001 cctggcagta atgcatgact ggcggtgatc aggttttctc cgacttctaa cgtttgtaat
    54061 aaaccggatt gatgaataat ggagatcact tttcgcgcct gccagaggga gagtcgggga
    54121 cttttgcgca acgttaaata gcaaaaacct aaactgatgc cactactgac ctggctgacc
    54181 cgttgcagct cctccggttt gccgatggtg atttgcacgg gatgccgaca actggttccc
    54241 aaatcttttt cgaaaagcgg cggagttatc cagtactcaa ccgctttaag cagcggatga
    54301 cgcaaatggt aataatcatg gctctccagg cgcaccagct ccgctgtgaa ttgtgttaag
    54361 cgaaaaggac cagtgccgat cagtgggaat tgcggatgcg ccagatggct gcaatagctc
    54421 gccagccggt gcgcaagcca gtaatcgggg cgatgtaaaa agaaggtcag acactgcgga
    54481 tgggtgactt caatacgctt cacgctaata aataattgat ccagtgctgg cagttgtaac
    54541 agcatcaata atcgctggtg taagtgtgag gcttttactg catcgccgtt atgccagtgt
    54601 agggttgaac gaagataaaa gtcccagcgt aacccgtcag tagaggtttc ccagtgatgc
    54661 gctaaatcgc caatcgggcg ctgagtatta ttatcgaagc gggtcaggcc ggaaaatatc
    54721 tgcccggcga gatgctgctc ggcacggccg ggcaaaaagc ctggttgtag cggttcgagc
    54781 gggcgatagt agggaatacg caacgtgggt gtatcgtttt gccattgtcc gcccataaac
    54841 ggctgtaaca gagtgcgtag ctcacctggg gccagttgcg ccagctccag cacatcttgc
    54901 tgctttccgg tttccagtgc ctgttccatc atcgcattgc gtagcgattc cggcgtgacc
    54961 agaaagcgta attgtccgcg ctttccgcgt cctgactgcg cctgccactc cagccatccc
    55021 gcctcctgtg cctgacgcaa cagcgtacga acatggcgtt cgctgcaaaa acagcgttcg
    55081 gccagttcgc tgacggtgac ggtttgcggc tttccggctg aaggttgcca cagacgttga
    55141 tactggttaa gacggttgag caatcgcata taaacccgga acaatattat ttaactattc
    55201 actattactt ccgtatatat caggtgatac tcaatctcca ttaaccgtgt cacagagtgg
    55261 agaaagaaat ggctcgtctg gcagcatttg atatggatgg cactttattg atgcctgacc
    55321 atcatttagg tgagaaaacc ctctctactt tggcgcgact gcgtgaacgc gacattaccc
    55381 tcacttttgc cacggggcgt catgcgctgg agatgcagca tattctcggg gcgctatcgc
    55441 tggatgcata tttgattacc ggcaacggaa cgcgcgtgca ttctctggaa ggtgaacttt
    55501 tacatcgtga tgatttacct gcggatgtcg cggagctggt gctgtatcag caatgggata
    55561 cccgagccag catgcatatc ttcaatgacg acggttggtt taccgggaaa gagatccctg
    55621 cgttgttgca ggcatttgtc tatagcggtt ttcgttatca gataatcgat gtcaaaaaaa
    55681 tgccactcgg cagcgtcacc aagatctgct tctgtggcga tcacgacgat cttacacgct
    55741 tgcagatcca gctatacgaa gcattaggcg agcgtgcaca tttgtgtttt tccgccacgg
    55801 attgcctcga agtgctgccg gtgggctgca ataaaggcgc tgcattgacg gtgctgaccc
    55861 aacatctagg tttatcgttg cgcgattgca tggcctttgg tgatgcgatg aacgatcgcg
    55921 aaatgttagg cagcgtcggt agcgggttta ttatgggcaa tgcgatgccg caactgcgcg
    55981 cggagctccc gcatttaccg gtgattggac attgccgaaa tcaggctgtc tctcactatt
    56041 tgacgcactg actggactat ccacatctac cttattcccc cgaataatga gatcccttcc
    56101 agcaccgggc aattgcccgg ttttttttgc gttgaatttg tcattttgtg ccgtggtgtt
    56161 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
    56221 aattctctgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
    56281 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
    56341 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
    56401 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
    56461 tcacagtagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
    56521 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
    56581 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
    56641 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
    56701 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
    56761 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
    56821 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
    56881 actaccgggc agatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
    56941 ttcctgcgtt acgtctggcg ggtattgctg tttggtgcgt cttatcaact ggctgttgaa
    57001 ctgcgtgaag attattaccg tcagctaagc cggcagcatc ctgagtttta cctgcgtcat
    57061 cgcacgggtg acctcatggc tcgtgcgacc aatgacgtcg atcgcgtcgt gtttgcagcc
    57121 ggagaagggg tgctgacgct ggtggattca ctggtgatgg gctgcgctgt gttgattatg
    57181 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
    57241 gcgatcatga tcaagcgcaa cggcgatgcc ttgcatgaac gctttaagct ggcacaggcg
    57301 gcgttttcta gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
    57361 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
    57421 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
    57481 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
    57541 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
    57601 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcata cagccgtatt
    57661 cgcgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
    57721 cgtggcgaac tggatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
    57781 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
    57841 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt cagcgagggg
    57901 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
    57961 ctggcggtag ttagccagac accgttcctt ttttccgaca ccgtagcgaa caacatcgcg
    58021 ttgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
    58081 catgacgata ttttgcgtct accgcaaggt tacgatacag aagtgggcga gcgcggcgtg
    58141 atgctttccg gtgggcaaaa acagcgtatc tccattgctc gagcgttatt agtcaacgcg
    58201 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagaac
    58261 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
    58321 tcggcactga cggaagccag tgaaaatgta atgaacg
//
LOCUS       DAJVTY010000007        47207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVTY010000007 DAJVTY010000000
VERSION     DAJVTY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47207
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.007"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            17..754
                     /locus_tag="OQQ66_000427"
     CDS             17..754
                     /locus_tag="OQQ66_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1903474.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLANTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            865..1875
                     /gene="pyrD"
                     /locus_tag="OQQ66_000428"
     CDS             865..1875
                     /gene="pyrD"
                     /locus_tag="OQQ66_000428"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1903475.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2049..2591
                     /gene="zapC"
                     /locus_tag="OQQ66_000429"
     CDS             2049..2591
                     /gene="zapC"
                     /locus_tag="OQQ66_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1903476.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OQQ66_000430"
     CDS             complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OQQ66_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1903477.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3941..6049
                     /gene="rlmKL"
                     /locus_tag="OQQ66_000431"
     CDS             3941..6049
                     /gene="rlmKL"
                     /locus_tag="OQQ66_000431"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1903478.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPLKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6061..7968
                     /locus_tag="OQQ66_000432"
     CDS             6061..7968
                     /locus_tag="OQQ66_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1903479.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8098..9351
                     /gene="pqiA"
                     /locus_tag="OQQ66_000433"
     CDS             8098..9351
                     /gene="pqiA"
                     /locus_tag="OQQ66_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1903480.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9356..10996
                     /gene="pqiB"
                     /locus_tag="OQQ66_000434"
     CDS             9356..10996
                     /gene="pqiB"
                     /locus_tag="OQQ66_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1903481.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            10993..11556
                     /gene="pqiC"
                     /locus_tag="OQQ66_000435"
     CDS             10993..11556
                     /gene="pqiC"
                     /locus_tag="OQQ66_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1903482.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11812..11979
                     /gene="rmf"
                     /locus_tag="OQQ66_000436"
     CDS             11812..11979
                     /gene="rmf"
                     /locus_tag="OQQ66_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1903483.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OQQ66_000437"
     CDS             complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OQQ66_000437"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1903484.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12636..14396)
                     /locus_tag="OQQ66_000438"
     CDS             complement(12636..14396)
                     /locus_tag="OQQ66_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1903485.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14582..15034
                     /gene="matP"
                     /locus_tag="OQQ66_000439"
     CDS             14582..15034
                     /gene="matP"
                     /locus_tag="OQQ66_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1903486.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OQQ66_000440"
     CDS             complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OQQ66_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1903487.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OQQ66_000441"
     CDS             complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OQQ66_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1903488.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17235..17864
                     /gene="sxy"
                     /locus_tag="OQQ66_000442"
     CDS             17235..17864
                     /gene="sxy"
                     /locus_tag="OQQ66_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1903489.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17827..19989)
                     /gene="yccS"
                     /locus_tag="OQQ66_000443"
     CDS             complement(17827..19989)
                     /gene="yccS"
                     /locus_tag="OQQ66_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1903490.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(19999..20445)
                     /locus_tag="OQQ66_000444"
     CDS             complement(19999..20445)
                     /locus_tag="OQQ66_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1903491.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20568..22622
                     /gene="helD"
                     /locus_tag="OQQ66_000445"
     CDS             20568..22622
                     /gene="helD"
                     /locus_tag="OQQ66_000445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1903492.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(22654..23112)
                     /gene="mgsA"
                     /locus_tag="OQQ66_000446"
     CDS             complement(22654..23112)
                     /gene="mgsA"
                     /locus_tag="OQQ66_000446"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1903493.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23208..23870)
                     /locus_tag="OQQ66_000447"
     CDS             complement(23208..23870)
                     /locus_tag="OQQ66_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1903494.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24043..24456
                     /locus_tag="OQQ66_000448"
     CDS             24043..24456
                     /locus_tag="OQQ66_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1903495.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24501..24818)
                     /gene="hspQ"
                     /locus_tag="OQQ66_000449"
     CDS             complement(24501..24818)
                     /gene="hspQ"
                     /locus_tag="OQQ66_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1903496.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24876..26066)
                     /gene="rlmI"
                     /locus_tag="OQQ66_000450"
     CDS             complement(24876..26066)
                     /gene="rlmI"
                     /locus_tag="OQQ66_000450"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1903497.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26161..26439
                     /gene="yccX"
                     /locus_tag="OQQ66_000451"
     CDS             26161..26439
                     /gene="yccX"
                     /locus_tag="OQQ66_000451"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1903498.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26436..26765)
                     /gene="tusE"
                     /locus_tag="OQQ66_000452"
     CDS             complement(26436..26765)
                     /gene="tusE"
                     /locus_tag="OQQ66_000452"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1903499.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26856..27515)
                     /gene="yccA"
                     /locus_tag="OQQ66_000453"
     CDS             complement(26856..27515)
                     /gene="yccA"
                     /locus_tag="OQQ66_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1903500.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27722..27809)
                     /locus_tag="OQQ66_000454"
     tRNA            complement(27722..27809)
                     /locus_tag="OQQ66_000454"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27773..27775),aa:Ser,seq:tga)
     gene            28236..29354
                     /gene="hyaA"
                     /locus_tag="OQQ66_000455"
     CDS             28236..29354
                     /gene="hyaA"
                     /locus_tag="OQQ66_000455"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1903501.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29351..31144
                     /gene="hyaB"
                     /locus_tag="OQQ66_000456"
                     /pseudo
     CDS             29351..31144
                     /gene="hyaB"
                     /locus_tag="OQQ66_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            31163..31870
                     /gene="hyaC"
                     /locus_tag="OQQ66_000457"
     CDS             31163..31870
                     /gene="hyaC"
                     /locus_tag="OQQ66_000457"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1903502.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31867..32454
                     /locus_tag="OQQ66_000458"
     CDS             31867..32454
                     /locus_tag="OQQ66_000458"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1903503.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32451..32849
                     /gene="hyaE"
                     /locus_tag="OQQ66_000459"
     CDS             32451..32849
                     /gene="hyaE"
                     /locus_tag="OQQ66_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1903504.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32846..33703
                     /locus_tag="OQQ66_000460"
     CDS             32846..33703
                     /locus_tag="OQQ66_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1903505.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33837..35381
                     /gene="appC"
                     /locus_tag="OQQ66_000461"
     CDS             33837..35381
                     /gene="appC"
                     /locus_tag="OQQ66_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1903506.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35393..36529
                     /gene="appB"
                     /locus_tag="OQQ66_000462"
     CDS             35393..36529
                     /gene="appB"
                     /locus_tag="OQQ66_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1903507.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36542..36634
                     /gene="cbdX"
                     /locus_tag="OQQ66_000463"
     CDS             36542..36634
                     /gene="cbdX"
                     /locus_tag="OQQ66_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1903508.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36714..38012
                     /gene="appA"
                     /locus_tag="OQQ66_000464"
     CDS             36714..38012
                     /gene="appA"
                     /locus_tag="OQQ66_000464"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1903509.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38127..40307)
                     /gene="etk"
                     /locus_tag="OQQ66_000465"
     CDS             complement(38127..40307)
                     /gene="etk"
                     /locus_tag="OQQ66_000465"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1903510.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40327..40773)
                     /gene="etp"
                     /locus_tag="OQQ66_000466"
                     /gene_synonym="yccY"
     CDS             complement(40327..40773)
                     /gene="etp"
                     /locus_tag="OQQ66_000466"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1903511.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40761..41900)
                     /locus_tag="OQQ66_000467"
     CDS             complement(40761..41900)
                     /locus_tag="OQQ66_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1903512.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41946..44042)
                     /locus_tag="OQQ66_000468"
     CDS             complement(41946..44042)
                     /locus_tag="OQQ66_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1903513.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44042..44788)
                     /locus_tag="OQQ66_000469"
     CDS             complement(44042..44788)
                     /locus_tag="OQQ66_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1903514.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVDPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44785..45429)
                     /gene="gfcB"
                     /locus_tag="OQQ66_000470"
     CDS             complement(44785..45429)
                     /gene="gfcB"
                     /locus_tag="OQQ66_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS1903515.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45536..45841)
                     /locus_tag="OQQ66_000471"
     CDS             complement(45536..45841)
                     /locus_tag="OQQ66_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCS1903516.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46283..46495)
                     /gene="cspH"
                     /locus_tag="OQQ66_000472"
     CDS             complement(46283..46495)
                     /gene="cspH"
                     /locus_tag="OQQ66_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1903517.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46781..46993
                     /gene="cspG"
                     /locus_tag="OQQ66_000473"
     CDS             46781..46993
                     /gene="cspG"
                     /locus_tag="OQQ66_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS1903518.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47004..47192
                     /locus_tag="OQQ66_000474"
     CDS             47004..47192
                     /locus_tag="OQQ66_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1903519.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11622 a        11729 c        12410 g        11446 t
ORIGIN      
        1 cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa
       61 cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg aattgcgggc
      121 aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat cgatatctat
      181 tttgctggct aataccagtc aggaatcctg gctgattaac agtaaaatca accgcccaac
      241 gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg ctccgccact
      301 tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat
      361 cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc catccggcaa
      421 agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc
      481 cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc
      541 gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt
      601 aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc gtcagacaag
      661 ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg attacgacgg
      721 gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac gtttgcgttt
      781 tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgt ccccggtttg
      841 cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag
      901 ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat tacaggaacg
      961 ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg
     1021 acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgac
     1081 gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag
     1141 ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt
     1201 atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa ggcccattat
     1261 gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga gcagggcaaa
     1321 gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat cgccatcaat
     1381 atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc gctggatgat
     1441 ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg
     1501 ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca ggttgccgat
     1561 agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact cgatcgttct
     1621 cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg tccgcttcag
     1681 ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg cttaccgatc
     1741 atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggtgcc
     1801 tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc
     1861 gttacccata tctaattatt tcttcgaatt cctctttata agataatcag ggctttattt
     1921 tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg tacttttatt
     1981 gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg
     2041 agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg
     2101 atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaga
     2161 tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt
     2221 tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg
     2281 tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa agctggcatt
     2341 ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga
     2401 gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcactat
     2461 gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca
     2521 ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac
     2581 aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag caaagaatgg
     2641 tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct aaaatctgaa
     2701 cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt
     2761 ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa
     2821 tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc
     2881 cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc
     2941 cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc
     3001 ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc
     3061 tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa
     3121 tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc accagattgg
     3181 ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg
     3241 cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc
     3301 gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa
     3361 atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc
     3421 aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc
     3481 catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccatct
     3541 ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata aagatgcgat
     3601 cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg cgcatcgatt
     3661 taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata
     3721 agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc
     3781 gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct cgaaatcctc
     3841 acgtacttgt gtacgctccg gtttctccgc gctgtccatg tccagactga ctgcaacaat
     3901 tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag
     3961 tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga
     4021 atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag
     4081 cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta
     4141 cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc
     4201 tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta
     4261 cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg
     4321 tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac
     4381 cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg
     4441 tgctggtatt gcgccgctca aagaaaccct ggcagccgct attgtgatgc gatccggctg
     4501 gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc
     4561 ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg gctttagcgg
     4621 ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc
     4681 ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg acgcacgggt
     4741 gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga
     4801 ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gcacagtgtt
     4861 gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag
     4921 cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt tgtttagtgc
     4981 ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa
     5041 cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc cagacagcaa
     5101 accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca aaaaattcga
     5161 gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctgccgga
     5221 atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt atgcaccgcc
     5281 aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat
     5341 ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg
     5401 caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa
     5461 cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc tcgatcaccg
     5521 tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca acctgttctc
     5581 ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca ccaccaccgt
     5641 ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcctaac
     5701 cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga
     5761 acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga
     5821 tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg
     5881 tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg
     5941 cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt
     6001 cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta
     6061 atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat
     6121 aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg caacggcgcg
     6181 ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt
     6241 atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag
     6301 ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc
     6361 tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg
     6421 gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa ccgcatcaac
     6481 gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct ttccggcggc
     6541 tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt
     6601 gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg gttccttaaa
     6661 accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg
     6721 acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag
     6781 tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat
     6841 cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt
     6901 aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga acgtcgcgaa
     6961 gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc
     7021 gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc
     7081 caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg caaaaccacg
     7141 ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca cgttggcacc
     7201 aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg
     7261 atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta
     7321 ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg
     7381 ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac
     7441 ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa
     7501 gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc
     7561 gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc
     7621 ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg
     7681 gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc
     7741 aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg
     7801 gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt cagtcagcca
     7861 catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa
     7921 gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt
     7981 cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc
     8041 atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg agtaccaatg
     8101 tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg
     8161 ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc
     8221 gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc actgttcatg
     8281 ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa
     8341 attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc
     8401 tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg
     8461 gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa
     8521 ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa
     8581 ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt
     8641 gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc
     8701 ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg
     8761 cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt
     8821 agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta
     8881 acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta
     8941 ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct
     9001 tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc
     9061 gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg
     9121 catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt
     9181 atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg
     9241 ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac
     9301 ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga
     9361 atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt
     9421 tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca gccatcaggg
     9481 accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat
     9541 taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac
     9601 gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagacac
     9661 cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc tgggaacgct
     9721 gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata
     9781 cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct
     9841 cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg
     9901 ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca gttatcaact
     9961 gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct ggaaagatag
    10021 tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct cattgacaac
    10081 gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag ggcagccagt
    10141 ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta
    10201 caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg
    10261 agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc
    10321 gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga
    10381 gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc accttggcga
    10441 attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact
    10501 gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa
    10561 tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc aacgactgat
    10621 ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag
    10681 tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa
    10741 caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg
    10801 tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt
    10861 ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct
    10921 caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa
    10981 gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc
    11041 agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa
    11101 agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat
    11161 ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac
    11221 aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt
    11281 acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca ggacacgctc
    11341 aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag
    11401 tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga aggagtgcaa
    11461 actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag tcaggaagcc
    11521 gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt aagtataacc
    11581 gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc
    11641 tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg
    11701 aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt
    11761 agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga
    11821 caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga
    11881 cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc
    11941 tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa
    12001 cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc agaaggcaga
    12061 cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac gaccatcaac
    12121 cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc gtttaaagtg
    12181 aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc
    12241 aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctactag
    12301 ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa tgaagtggca
    12361 tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa
    12421 gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt tcggtgctgg
    12481 caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa ggtcttcttt
    12541 tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc
    12601 aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc
    12661 caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt
    12721 tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat
    12781 aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc
    12841 gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg gataatgaca
    12901 ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc gattttctca
    12961 tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata
    13021 ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc agccatcgag
    13081 gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg
    13141 atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc
    13201 gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc accatcgcca
    13261 atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat
    13321 tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt
    13381 tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc
    13441 tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg
    13501 acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg
    13561 gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg ggcaggtaag
    13621 tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga ctccgcatcg
    13681 acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc
    13741 atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat
    13801 ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa
    13861 cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta
    13921 cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta
    13981 atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca
    14041 gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg
    14101 atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc gctttgtagc
    14161 gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc cttagccagc
    14221 ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagccgcggt
    14281 tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc aaatatttcc
    14341 tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt
    14401 gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg
    14461 tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg gagattcttt
    14521 tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc
    14581 tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa
    14641 gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg cccaggaagc
    14701 cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct ggattgacaa
    14761 gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca gacggaagcg
    14821 ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg aatttattgt
    14881 ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt
    14941 tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa
    15001 gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta
    15061 aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact taagcctgcg
    15121 gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca
    15181 ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
    15241 tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt
    15301 aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga
    15361 tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct
    15421 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt
    15481 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
    15541 gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa acacccaagc
    15601 tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc
    15661 actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccgccagcga
    15721 agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta gtgtctgcac
    15781 gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca
    15841 gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt
    15901 acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt
    15961 aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg ttgttgatga
    16021 aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt
    16081 tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg
    16141 tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag
    16201 ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc
    16261 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    16321 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atcttttttg
    16381 tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    16441 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    16501 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg
    16561 agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    16621 aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacact
    16681 gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag gggagagctg
    16741 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag
    16801 tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa
    16861 tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat
    16921 aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa
    16981 cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta
    17041 ctgtatgaat gtacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc
    17101 ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa
    17161 gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt
    17221 ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc
    17281 tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg
    17341 acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa
    17401 gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat
    17461 ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg
    17521 tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc
    17581 gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagg
    17641 tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt tggttgcgac
    17701 tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa ggtgccatta
    17761 tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca gaatgggctg
    17821 actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc gttgcagacg
    17881 accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca gaggttcttt
    17941 gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta atgcctcatt
    18001 gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc aaactgcgtc
    18061 atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga
    18121 gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt cgcgaatttg
    18181 cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct cagcatcacg
    18241 gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta
    18301 ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt cgagcatgcg
    18361 cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc acgcaatggc
    18421 acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt cacccagtaa
    18481 gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt
    18541 acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc cttccagtga
    18601 tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac caataatcct
    18661 taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag
    18721 caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc cgaagcacaa
    18781 caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct
    18841 aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct cattttcgtc
    18901 attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt gggcatcaat
    18961 ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt
    19021 atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc
    19081 atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg cctggccctg
    19141 catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa
    19201 tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg cgacaaagta
    19261 ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag
    19321 cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag ccaaatcgta
    19381 cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact tgagctcaag
    19441 ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa
    19501 cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat
    19561 cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa tggcgatcag
    19621 caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc
    19681 gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca gcaattctac
    19741 tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc
    19801 cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt
    19861 cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat
    19921 acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag gacttagcat
    19981 aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg
    20041 cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag cggcaatttt
    20101 aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga tgcccgttgc
    20161 aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt
    20221 accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga catgaatagc
    20281 ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg
    20341 taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca gagtggtggc
    20401 aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac
    20461 tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc
    20521 tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg gaactgaaag
    20581 cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg
    20641 ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat caattactgg
    20701 cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg ccggacgaaa
    20761 aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac catcatcttg
    20821 atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc
    20881 agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa
    20941 cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccggttaacc
    21001 gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag
    21061 atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg cttaccgagt
    21121 atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag gcccgggcag
    21181 tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg
    21241 tgctggtggc ccgtgcaggc tggttgctgg cacgtggtga agcgtcccct gagcaaattt
    21301 tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt cgcgaacggc
    21361 tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat attattcagc
    21421 agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct gcccgtcatg
    21481 aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct
    21541 ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg
    21601 atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt ctgatgcgga
    21661 tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt
    21721 tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt gcgctgaagg
    21781 cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt ctggagaaag
    21841 gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat atctcgccgc
    21901 agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg ttgttcgctg
    21961 ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg
    22021 ctttccatga aaactttggt gaaggcgaac gttgtgattt agacacgact taccgtttta
    22081 acagtcgtat cggtaaggtg gcaaaccgtt ttattcagca gaacccaggc cagctgaaaa
    22141 agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg gatgagagtc
    22201 aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag cgcattctga
    22261 tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca acacgctggc
    22321 cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag gcggattacg
    22381 tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg cgggagtcga
    22441 ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa gaacggcggt
    22501 taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt aacaaagaga
    22561 atccctctcc ctttgttgaa atactgaaaa atctggatgt gccggtggcg agaaaaccgt
    22621 aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg cgagataacg
    22681 ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact ggattatgaa
    22741 gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca gacgcagcaa
    22801 ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga aaatcaatac
    22861 atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca ttgggccact
    22921 caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt
    22981 tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc tcatcagcat
    23041 ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt
    23101 cgtcagttcc ataatgtaca tccgtagtca actttcctac agattactgt aagcacttat
    23161 cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca ggaagatggt
    23221 tgtttttccg cccactgcag gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa
    23281 tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc cagctcggaa
    23341 acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg
    23401 cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt
    23461 gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc atccaacaat
    23521 tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag gcgaggcagg
    23581 cgaaaattga cctggttgat gagctgggta ttaaaactga ccactagcgg tggggagata
    23641 tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa cactaactga
    23701 tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc
    23761 ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg
    23821 gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat aggtagctcc
    23881 tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat acttactaac
    23941 atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat ggttggcatg
    24001 tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga aaccgatatt
    24061 gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga caaacccgat
    24121 cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcatgt cattccggtt
    24181 tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac gctggcagac
    24241 gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgtg
    24301 gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg ggtgattaat
    24361 gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga tcgctgcccg
    24421 gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag gcaaaaacct
    24481 tccggggatt cgttcaggga tcagttacgc agacgcgggg cctggagttg tttgcggatg
    24541 gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa ttcgctactt
    24601 agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc catcactacg
    24661 tgataccacg gagcggcgcg aagctcgtca tttaccgcca gctcatcagg cgacggttca
    24721 gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa cagggaatgg
    24781 cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg ggaaatcttt
    24841 acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca ggcaaaccct
    24901 ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc ctgacggaac
    24961 tgctctataa attgtacatc acggccggca tcaattgcgg catccgcgat gattttctga
    25021 aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc gccttcattc
    25081 agcaactgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc catcaactgg
    25141 cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga
    25201 tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc cttgctcaga
    25261 tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcctg ggaggtatca
    25321 acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc accggtatag
    25381 gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag gcggctgtct
    25441 cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag cagcttcatg
    25501 ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag gcccagggtc
    25561 agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat cgcacattcc
    25621 gggtacagcg tttgcagggc actaactaat gccgcgcgct gatattctgc cccagcactc
    25681 agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg caggccatca
    25741 gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag ccagtcacgc
    25801 catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg
    25861 tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc gcgtgctaac
    25921 cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt accttccatg
    25981 cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt ttcgcgccct
    26041 ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa gaaatgggcg
    26101 tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag gagagcagca
    26161 atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc
    26221 tacaccacac agtacgaagc gaaaagactg ggtttaaccg ggtacgccaa aaatcttgac
    26281 gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa attaatgcag
    26341 tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat
    26401 catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac cggttttggc
    26461 aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg gtataaatag
    26521 cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag catacgaatc
    26581 gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt
    26641 tccggtgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg ctcactccac
    26701 tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc ttcgaagatc
    26761 agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac aaagccccgc
    26821 ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc gaagcccaga
    26881 atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac cgtggcacga
    26941 atatagttcg tttcaccgcc atgaacgatg ttgctggttt caaacaaaat agcgccagag
    27001 gagatcaaaa tgaagaccgc actgatcgcc agatgcagag caggcagctg caggaagata
    27061 ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc gccgaggaac
    27121 gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac taacgccgtt
    27181 ccgcccagag ccatagcgat tacgtccccc attccggcag acagataggt gttcagaata
    27241 ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat cccggtcggc
    27301 ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag cgtcagaatc
    27361 agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc
    27421 agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct aagcagtgat
    27481 gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat gacagatgta
    27541 attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac ccatctttac
    27601 gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa atataacgcc
    27661 ctgagaattt cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc cttatcttat
    27721 ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc
    27781 ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac atcccgatgc
    27841 ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc
    27901 gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt tactcgttta
    27961 gcaaccggct aaacatcccc accgcccggc aaaaagaaaa ataggtccat ttttatcgct
    28021 aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa
    28081 caatactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt cagcatctca
    28141 cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa gcgcccggtg
    28201 tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa acattttacc
    28261 aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt agtctggctg
    28321 ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg ctggagaaca
    28381 aaccccgcat tccggtggta tggatccacg gtctggaatg cacctgctgt accgaatctt
    28441 ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt tccctcgatt
    28501 atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttc gaagacatca
    28561 tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg ctgggcgagc
    28621 aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa cgtgccgctg
    28681 ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc gtgcaggccg
    28741 cgcggcccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac aaacccatta
    28801 tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt acttacatgg
    28861 tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgctgatg ttctatggtc
    28921 agcgaatcca cgataaatgc tatcgccgcg cccacttcga cgccggagag ttcgtccaga
    28981 gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc
    29041 ctaccaccta taacgcctgt tcctccacac gctggaatga tggcgtttct ttcccaatcc
    29101 agtctggtca cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc ggttcgttct
    29161 acagccgcgt ggtcgatatt ccgcaaatgg gtactcattc caccgccgat accgtcggtt
    29221 taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg
    29281 accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca ggcaatgagg
    29341 ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata atgccggacg
    29401 ccgcctggtg gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg aagtgaatat
    29461 taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc gcgggctgga
    29521 gatcatccta caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg
    29581 cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg ctatcggtat
    29641 taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc tctggtgcca
    29701 cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg tgttagatgc
    29761 gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct cttggccgaa
    29821 atcatcccct ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg ttgaaggcgg
    29881 gcagttgggg atcttccgca atggctactg ggggcacccg cagtataaac tgccgccaga
    29941 agctaacctg atgggctttg cccactatct cgaagctctc gatttccagc gtgaaattgt
    30001 caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg tcggcgggat
    30061 gccttgcgcc atcaacattg acgaaagcgg cgcggtcggg gcagtcaata tggaacgcct
    30121 gaacctggtg cagtcaatta tcacccgcac ggcggacttc attaacaacg tgatgatccc
    30181 cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca ctggtctttc
    30241 tgataaatgc gttctcagct acggcgcatt cccggatatt gccaacgact ttggcgagaa
    30301 aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg tgctgccagt
    30361 ggatttggtt gatccgcagc aggtgcagga gtttgtcgac cacgcctggt atcgatatcc
    30421 caacgatcag gtcgggcgtc atccgttcga tggcatcacc gacccgtggt acaaccccgg
    30481 cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac gctactcgtg
    30541 gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg cgcgcacgtt
    30601 aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca tgatgtcggc
    30661 gttgaacctg ccgctttccg gtatccagtc aacgttaggc cgcattttgt gccgcgcgca
    30721 cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gacaagctga tgaccaacct
    30781 gaaaaacggc aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa cctagccgac
    30841 agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc actgggccgc
    30901 cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct ggaacgccag
    30961 cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga acaccaaaat
    31021 ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg acccgtgcct
    31081 cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctccg tgcaggtgcg
    31141 ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca gccactatgt
    31201 ctttgaagcg ccagtccgca tctggcactg gttgacggtg ttatgcatgg cggtgttgat
    31261 ggtcaccgga tactttatcg gcaagccgct accttccgtc agcggcgagg cgacgtatct
    31321 gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt ttaccgtggt
    31381 tttgctgatg cggatctact gggcttttgt tggcaatcga tactcccgcg agctgtttat
    31441 cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc gctggtatct
    31501 gtttctggca aaacgtccga gtgccgatat aggccataat cccatcgccc aggcggcgat
    31561 gttcggctat ttcctgatgt cggtctttat gatcatcact ggttttgcgc tgtacagcga
    31621 acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt tctactggac
    31681 gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc tgattggcgc
    31741 gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg acggcacggt
    31801 gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa gtaacaagga
    31861 gtgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct gtgggccgat
    31921 gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg gcccgagtat
    31981 gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt cgaaagcgcc
    32041 agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaac gctgcgaacc
    32101 tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct gcatcagaac
    32161 agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc acatattgcc
    32221 ctcgtcgggc tgcaacccgc gatgctcgac gactacggcg gcagcctgag cgaactggca
    32281 cgggagcaac tgctcgctgc ggaacaatcg gcgctggcgc agttggctgc gtggggaagt
    32341 gtgccgcaac tggctaatga atcgcgttgt ctcaattatg actgtctgtc gatggaaaat
    32401 tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg atgagcaacg
    32461 acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg ccggtcagtg
    32521 aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta ttaagcagtg
    32581 acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa ttactgcacg
    32641 agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc gaagccatcg
    32701 gcgatcgctt tggcgtcttt cgctttccta ccactttagt gtttaccggc ggaaactatc
    32761 gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg cgcgggcttg
    32821 tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc tggggccagg
    32881 aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct gtcaggtgaa
    32941 tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg tgattgcgct
    33001 gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg aggtgctggc
    33061 agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc ttctcgggga
    33121 aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt aaaagtgaaa tacaggaggc
    33181 gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc tggaggacaa
    33241 actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa atctcttgcc
    33301 gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac cgttggcgca
    33361 tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta atctgacgca
    33421 attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc cgggaaatat
    33481 tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt tacgccatgt
    33541 ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt atgaaatctg
    33601 cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg cgcagcggct
    33661 tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg gtaagtatcg
    33721 ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt ttcaggataa
    33781 agagggagat ctaacattat cgggttattt ttctctcttc gcctacagga gtgcgcatgt
    33841 gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat cactttttat
    33901 ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc atttacgtgg
    33961 tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc ttcggtatca
    34021 attttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt actaactggg
    34081 cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg gaagcattaa
    34141 tggccttctt cctcgaatcc acctttgtcg ggcttttctt cttcggctgg cagcggctga
    34201 ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat ctctctgcat
    34261 tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat tttgatatcg
    34321 acaccctacg tatggagatg accagcttca gcgagctggt ctttaacccg gtcagccagg
    34381 tgaaatttgt gcacaccgta atggcgggct acgtgaccgg ggccatgttt attatggcga
    34441 tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc tcgtttgcca
    34501 tcggttccgt cttcggtact ctggcgatta tcggcacgct acaactcggg gacagttctg
    34561 cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg gagtggcaaa
    34621 cggaacctgc tcctgcaccg ttccatgtgg ttgcctggcc ggaacaggat caagagcgta
    34681 acgcctttgc catcaaaatt cccgcgctgt tagggatcct cgccactcac tcattagata
    34741 aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg caacgcggac
    34801 gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc
    34861 aggcattccg ggaactggaa ggtgacctgg gctacggcat gttgctctcc cgctatgcgc
    34921 cggatatgaa tcatgtcaca gccgcacagt accaggcggc gatgcgtggc gcgatacctc
    34981 aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc ctgctgctac
    35041 tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag catcgctggg
    35101 tgctgaaaat ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa gccgggtggt
    35161 ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg acatactccg
    35221 cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc gtagggcttt
    35281 acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt ctggggccga
    35341 gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aagaagaaaa tcatgtttga
    35401 ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg tggtctttat
    35461 gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg cgcgtaatga
    35521 tgatgaacgc cggatagtga taaacagcgt tggtgcacac tgggaaggca accaggtctg
    35581 gttgatcctc gctggtgggg cattatttgc cgcctggccc agagtgtatg cagcggcgtt
    35641 ttccggcttt tatgtggcga tgatcctggt gctgtgctca ctgttcttcc gcccgctggc
    35701 ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg acgccggtct
    35761 ggtcatcggc agtctggtgc cgccggtagt cttcggtatc gccttcggca acttgttgct
    35821 cggcgtgccg tttgccttca caccgcaatt acgcgtggag tatctcggca gcttctggca
    35881 actgctgacg ccattccctt tattgtgcgg attgctcagc cttgggatgg taattttgca
    35941 aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgagtt cacagctggc
    36001 caccaaacgc gctgcactgt tggtgatgct gtgctttttg ctggcgggtt actggctgtg
    36061 ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt ccaatccgtt
    36121 aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg aatcgcccgt
    36181 tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga tggcgattta
    36241 tcgtggtcgc ccaggttggg gatttttaat ggcgtcattg atgcaatttg gcgtgatttt
    36301 cacggcaggc atcacgctgt tccccttcgt catgccgtca agcgtgagtc cgatctccag
    36361 cctgacgttg tgggacagca cttccagcca gctgacgctg agcattatgt tggtaatcgt
    36421 gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca aaatgtgggg
    36481 gcgcatgaca acagaaactc tccgccgtaa cgaaaacgag ttgtactaag gagcaggaac
    36541 aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt
    36601 gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taggcctgat gcggcgcatt
    36661 agcatcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata tcgatgaaag
    36721 cgatcttaat cccattttta tctcttctga ttccgttaac ctcgcaatct gcattcgctc
    36781 agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg
    36841 ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg ccaacctggc
    36901 cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcctat ttcggacatt
    36961 accaacgcca gcgtctggtg gccgacggat tgctggcaaa aaagggctgt ccgcagcctg
    37021 gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc gaagccttcg
    37081 ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc
    37141 ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataat gcgaacgtga
    37201 ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg catcggcaaa
    37261 cggcgtttcg cgaactggaa cgggtgctta attttccgca atcaaacttg tgccttaaac
    37321 gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga
    37381 gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggagatat
    37441 ttctcctgca acaagcacag ggaatgccag agccggggtg gggaaggatc accgattcac
    37501 accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttgcta caacgcacgc
    37561 cagaggttgc ccgcagccgc gccaccccgt tattggattt gatcatggca gcgttgacgc
    37621 cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagta ctgtttattg
    37681 ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc
    37741 ccggtcagcc ggataacacg ccgccaggtg gtgaactggt gtttgaacgc tggcgtcggc
    37801 taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta cagcagatgc
    37861 gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg accctggcag
    37921 gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg caaatcgtga
    37981 atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt cagttacctg
    38041 aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg aaaaggcgtt
    38101 cacgccgcat ccggccactt tcagttttac tctttctcgg agtaactata accgtaatag
    38161 ttatagccgt aactgtaagc ggtgctggcg cgtttaatca caccattgag gatagcgcct
    38221 ttaatattga cgcctgcctg ttccagacgc tgcattgaca aactcacctc tttggcggtg
    38281 ttcaagccaa aacgcgcaac cagcaggctg gtgccaacag aacgccccac gaccgcggca
    38341 tcactcaccg ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg gtcgttcgcc
    38401 cattccagta attgacgcat ccgatcgcgc atcagcagtt cagacgggtt aggtggcacc
    38461 tgaccgcgag taatcacatc aaagcctcct ttgccaaaat gctggatcac tttgttgagc
    38521 tcatctttac ctgccagata ttccgacaag ccatgttcat tactcacggt aaacaggtta
    38581 tgcgaataac cacggcgtaa gtcggcatca ataaataaca ctttttgatc ggactgggcg
    38641 atcaccgctg ccagagttga actgacaaac gttttaccac tgtctggcgt cgcaccggtg
    38701 atcatcagaa tgttattctc cgtctccatc atagcgaagt gcagactggt tcgtagcgca
    38761 cgtacggctt ccacagcaga atccgccggg ttatccaccg ccaggaaggg gatattttta
    38821 gtacgatggc gctgctgatt agaaaataaa tttttcttac gcagacgggt acgtttatcc
    38881 agccactcgg acattgggat agtggcataa acgctgatgc cgtgctcttc cagttgttcc
    38941 ggggcttcta caccacgacg caacatcgca cgcgccagca cggcacccac cgaaataaac
    39001 aggccaagaa taaaaccaag caccacgttc aacgcttttt tcggtttcac tggctgcggc
    39061 tgagtgactg ctgggtcgat aatccgcacg ttaccaatgg cactggattt cgaaatactc
    39121 aactcctgct ggcggttaag taattgcaga tataccgcac ggcccgcttc tacgtcacgg
    39181 cttaaacgta acacttcctg ttgagtggaa ggcattgccg atacccgctt attcaggcgt
    39241 ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg cacgataagt tgggtgatct
    39301 ttcttataca gctgggagat ctctgcctcg cggaaagtca gctcattgag ttgattatca
    39361 acgttcacaa tctgctcaag aacggctttg gcttctaggt taaggtcaac cgaatcgcgc
    39421 tgctggcgat aaacgttgag tttttcttcc gcctggtcca gctcgctgcg cacttcaggc
    39481 aactggcgct gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg agcgatattc
    39541 tgttgcaaat agttgttagc gatgctgttc agaatacgag taatcaactg gggatcatca
    39601 ccagtcatgg taagttccag catcccgctt tctttactgc gttcgctaac ggtaaaggtt
    39661 tcctgcaatg cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa ctgtgttcct
    39721 ggtttggcct taatgtccgc gatagtcagc gcaacgccat ctttttccag acgctggccg
    39781 accataccat tgacggtgaa ctcttcacct tccagtgtat agtggccgtt ttccccaacc
    39841 gtgagtgtca gttgctgatc ctgaccattc agttgtggaa tatgcatcca gctgatcgcc
    39901 agctcacctg gtttttcttt ggttaatctc gcccagccgc gacccacaat cggaaaatac
    39961 ttctgctcaa ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag aatcatgcgc
    40021 gattgcagca gttggatctc cggtgcagac tcgggcgatg agttagggat catatcgctc
    40081 aggccgctga gaatggcgtt gccctgtttt tgctcaacct ggaccagagt atctgcctga
    40141 taaattggtg tgcttaacag cgagtaagcg acagcgatca gcgtgaataa cgcggttacg
    40201 ctgataataa acttacggtg atcccataac tcgccgacca gacgaagcag atcgatctca
    40261 ttttcctgag tgctgcctgg tggcgtattc atatttttag ttgtcatcgt attttataaa
    40321 ctcaaattac cggctgaggc gcttcgccca ttcctgactg gcgcgctcca acataccgta
    40381 gacatgttca aatgcgtcct gacttttacg atagggatcc gggatctctt tctgttccag
    40441 ccattgccca aacagcattg tcttcccgcg gacctcggga gcgattgcgg taacctgagc
    40501 aatatgctct gactccattg ccagaatcag atcgtaattt cgtgccatct cagcagtgag
    40561 cttacgtccg gcatgtcctt caagagatac gccatgatta gcggcaacat ctgctgctgt
    40621 cgcatctgca gggtgtttta ccagaccatg aacacctgcc gatttgactt ttacgcctgg
    40681 cagacgttta cgcagcaagc gctcgccaat gggcgaacgg caaatgttgc cggtacaaac
    40741 caccaggatg gagttaaatt ttagttgggc catgtttgaa tccaccgtga agtctctgtc
    40801 aggtcatgca ccccggaaat cgtcggtacc agcagagaaa tcacacggtt ccaacgagcc
    40861 agcggcgcgg tagtgacata aacaatgtcg taaggttcaa gctggaattc cgttccgagt
    40921 atcattgccg atgcgtcctt cgcattcagc tgataaatat tggcgatttt gccattctgc
    40981 ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat cagcgacatc ctgattcata
    41041 ccctcagcgt tacccagcgc ttccgccagg gtcataccgc tgcggtccat tttcagcgtg
    41101 ctctgtttac ccacttcacc catcacgaag actttcagat catcgttgcg cggaataaat
    41161 aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt cgccacgctg catcagcgca
    41221 tagaggttca ctttggtctt tacgccattc tgggtgagca ccacgttgcg ccagtctgca
    41281 tctgcggtca gaccgcccgc ggcgttaatc gcatccatga tggtcagagg aatattggta
    41341 atcggctgct gacccgattt gctgacttcg ccggtgacgt acgctttttg cgatcggaac
    41401 gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg aatcgagacg cgctgtaatc
    41461 tcgttgcgta cttgggtaag tgttttcccg gccactttca ggcgaccaat ataggggtag
    41521 aaaatcgcac catcggcgtt aacccagttg ccggtatcac tggcgctacg gtattgccct
    41581 gcgggggtgg ttagttccgg gtgatcccag accgtaacca tcaacacatc accaatcccg
    41641 atacgatatt catagtttgc gatttgctgc tccagctccg ggttagcctg cgacataatc
    41701 ggtttggcgc gcaactgatc aatcaatcct ggcgtgacgg gatagatatt gaccattttg
    41761 tccaggtcat actgattgtc aggtagttca atgacatcct tgttcctggt cgataaattc
    41821 tgaccaggaa caagggtaca tgctgtcaaa gacagaacag ccaatgtcag taccgaaaac
    41881 ttaaaaatgt tctttttcat tggtttatat ttatcagtca atgtaatgtt atgaattacg
    41941 tcaggttatt gtccaaccgg tacttctcgc tctgccgtca ttggatagag ctggtatttg
    42001 cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc acgaaaccac cgcgcggttg
    42061 cggttaggtc caatggtcat caagtcgaat ggaatcgaga tataaaaacc tttactaaag
    42121 ccgccttcgc cgtagtcatc tttagatacg ttactgattg ccgcccatac ccctaccgcc
    42181 acgccgctgt caaagcgttt ggcgacgtcg atcgttgctc ctttatcttt tgccagatat
    42241 tgcccaacgc taagtttcat cagtacgcca ttgagcgtcg gcgggttcca gtaagccgtc
    42301 acgaaaccag ttggcgtgga ataatcggtg aagcgcatca tgttgtccca gtcacgttgc
    42361 ttcacgtagt taacgtccac acccagcgcc cagctggcat ctagcgggcg ataaagcagc
    42421 tcggaaccga caccggcgta catcgtttcc agataaccgc catacacctg gccatagaaa
    42481 ccattgccta agtcggcaaa gtagttcgcc tgcaagttgt tgagataaac gtcattgcga
    42541 acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag agtccgcggg caacagcgaa
    42601 gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa taccgccatc aagcagcagg
    42661 tggtcggtaa accagtagcg ggcactggac atcagcccca gctggaacat atagaaatct
    42721 tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag agtagctaaa gcgatcttca
    42781 cgaatacggt agccccgacc aaaggcagaa agatcttctg cttcaacacg ttgttgttga
    42841 agttgctctg attgaccagg cgctgtacca gccagctgtt tgcgcaggct ggctacgtcg
    42901 gtttcggtag tcaccatcgc catatgctcg cgcttttgcg tcacgctaat cttctcaacg
    42961 ccttgcggca ggttattcac cagaatccgg ttggcacgat cgaccgcttc gcgagaatca
    43021 cggtatttgt attgttgacc agacatatac agcgtcttat cgcgcagctg aatttccggt
    43081 gcttcaaaac ctgcgttgta cttcagcgcg gtaagttggt tcgctactgt ggtgtactgc
    43141 aatccttcag attcaggcgc aggttgatat gccggttttg gcgtatcgcg cagggcaggg
    43201 cgcagatcgt tgaaattggt ccgtaacgtg aagccaaaca tcaacgtgtt accgcgttca
    43261 taactcaggt tgagatctgc ccagctggca gcgcgataaa ctgcgccgac gttgaaatgg
    43321 cttgcctgag gcagtttgcc agcgaaatca ttctggtaat tgttgccatc gtattcgagt
    43381 ttcagacgca ggggattcca cggcgtttga tactcaatgc cgccaaagat ggaagccggg
    43441 ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt gagactctgc gcgatgacaa
    43501 tatttatcgt tcacccggca aaacgggttg gtaatattgc ccgcattacc ggcgtagccc
    43561 catgccatcc cgagggtgaa atcaaatggc cccgcttgct tgctggccac cagatactca
    43621 ccgtcaaaca gacccgtacc agcaatatca cgtttaccaa acgccacttg cggtagccag
    43681 taaccttctt cccacaggcg aagtttaaaa tcgaatgatt tgtctttgta tgactgatcg
    43741 ccgctgaaat cttcccactg gctatatttg cgtgtgcgca catccgtata acgaatggtg
    43801 ccttccagcc atgggaacag cgccacggag gtggagtaga accggtattg atcgttatcc
    43861 cggtagttga cgctgaattc accttccggt gcgatgcgcg catttggcat ctgcaacaat
    43921 cctgtgccgc caaagtctga ttgcgacgaa cccagcggat ccgggtaggt taatacttca
    43981 gcatgacagg cactggagac ggcaatggcc aggcagctta aaagataaga attcttcttc
    44041 atcagtcagg aacacgttgc gttaaaacag acacaatctg gtcatttaaa tcagcatatt
    44101 tttcgggcag tacatgcgca gaaaagccca accaaagctg gctgccaggc ggtgggtcca
    44161 catgtctttt attccacagg gcaacagggg cgacgacagt ctcaccctca ggcgtaatca
    44221 ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg atcttgcaga taatcggtaa
    44281 cgctacggcc tgcctgccag ggcaattgcc cggccccaga aaccgcaccc agcagagtaa
    44341 tagtcaccgg acgctgtacg gtatagagcg tatagtcccc gaccaacggg ggattgctgt
    44401 tctcatccac tcgaacaaaa tcagggtcca gtttgaccgg taagcgacca gtgatattga
    44461 gattgagtaa ctgctggcgc acagatttaa tcgttgccgc cacatcgtcg tcagcttctg
    44521 cttcccagga agcaagctgt gccatgacat gttggtagtc tttaagcgct ttagctttcg
    44581 ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg atcgcgcagt tgcggttgtg
    44641 tcaccaactg gacaacattt tccatggggc caacggaaag tgtctgttgt tcgccaggca
    44701 gataaatagt caccgttcct tgcgcaaagg catggggtgt cattacgtaa agtacgctgg
    44761 caatgaaata cgactgtaat ttattcatgg tgcgggttta agaaacgtca tttctacggg
    44821 aataacccct gcgcctaaca tttgccgact ttgacgaacc tggccagtag ccgaatcgac
    44881 ccagaatgtg ttttgccact gtttttctgg ccgcgtggaa gtcacctctt cctgccagac
    44941 attacacagc acattgcgac ccgcaatatt cagcgtctct aatccagcaa aggaaaaagt
    45001 agaactcagg gtcgcagagc gaaaatggtt gtcttcactc cagagaatat cgcgcgtcca
    45061 gcggctgcca tcttttattg ccagcgcgtt acgcagaggg tcctgcccgg aatttgtcac
    45121 ttccagcaga ttattattaa gtttgacggt ttttaaaaga cgtccattgt gggtaaccag
    45181 catggcgtta tcctgggata accatttgct ttgttcttgt tctatatatc ccagtaccac
    45241 aaagattcgt tgcccttcat taaggcgtaa atacatcgtg gaataaggca acgcctggat
    45301 ctgctgatcc gctacggtga tatcctgatt atcgaaaagg ctggcgcgaa atgtatcgac
    45361 catactttgc tggctgtgcg tacatcccgc aagaaatagt gcgaaaatcg ataaaataag
    45421 agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat gcaaatcttt aaagcccggt
    45481 acgaatatcg caaccgggta tcatttaact ttaatttcga cgcgttaaag cagtattagc
    45541 gtgtagtgga cgtggtggtt gaggtgttat gagaaccgcc atcgccactg tcatttgctg
    45601 caacaactgc caacgtcgcc acaccaacag ccgttgccac accgacaact gtcgccgtgc
    45661 cgacagcgcc agcagccacg ccaccagtgg tagtagcagt cgtggttgcg ccggtagtgg
    45721 tcgttgtggt gccagtagta cccgtcgttg cctcggtccc gtttgttgct tcaggggcag
    45781 caaacgcagc tggcgtcgtc aacatgaagg ccataaggat tgcagaaagc ttgtgtttca
    45841 taacttttcc tttattcatc gcatggacaa tacgtacgct tgagatcagg attgtctact
    45901 ttgagtataa catccttgtg aaatctccct ttcctcatac cgcttgcact atatactgcg
    45961 ggtaggaaag gcgcgcagag ggaaatataa gattgttaac tatccccctc tggaaatacg
    46021 acttgtgagc ataagataaa tcttagcaaa atacaaatta aagataagga aattgttacg
    46081 aaagctatta attattgctg gtaatatagt ttcaagtggt actataactg gctgttaaat
    46141 ggcatttata actattaggt gcagagatat tcgcttaaat ggattagttt ctttgtggaa
    46201 aagtaactga tgttataata tatgatgttg actattgcac tattccaata aaaccagtca
    46261 gctttaaaca agcagcgtca tattaagaga gataaacatt tgccgctgtt ggtcctcgca
    46321 ggccatttac gcggcaaaat tccacacgta atcctggtat aagcacttct gcgtcgcggg
    46381 gagtgaatgc ggaaatatgg acctgaactt ctttacgacc gtcggagggg ataatgaatc
    46441 ctttgccgct tttgcgatca aaggttttga caattcctgt cattttacgg gacaaacaaa
    46501 ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag ccagcgatat
    46561 ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat gcgttaatag
    46621 ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag ttctcattat
    46681 caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt ctgtattaaa
    46741 gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata aaatgactgg
    46801 tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg atgatggcag
    46861 caaagacgtt ttcgtccatt tcactgccat ccagagcaat gaattccgca cgctgaacga
    46921 aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg cagcgaacgt
    46981 tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt ccatgttgtc
    47041 atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaatcct ttgggcgcta
    47101 agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcacgctg caaatacgca
    47161 ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcg
//
LOCUS       DAJVTY010000008        43881 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVTY010000008 DAJVTY010000000
VERSION     DAJVTY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43881
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.008"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            85..627
                     /gene="lpfE"
                     /locus_tag="OQQ66_000475"
     CDS             85..627
                     /gene="lpfE"
                     /locus_tag="OQQ66_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCS1903520.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPIPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYSTNNFTS"
     gene            867..2558
                     /gene="eptB"
                     /locus_tag="OQQ66_000476"
     CDS             867..2558
                     /gene="eptB"
                     /locus_tag="OQQ66_000476"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1903521.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2650..2726
                     /locus_tag="OQQ66_000477"
     tRNA            2650..2726
                     /locus_tag="OQQ66_000477"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Pro,seq:cgg)
     gene            3470..5077
                     /gene="dppA"
                     /locus_tag="OQQ66_000478"
                     /pseudo
     CDS             3470..5077
                     /gene="dppA"
                     /locus_tag="OQQ66_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5385..6404
                     /gene="dppB"
                     /locus_tag="OQQ66_000479"
     CDS             5385..6404
                     /gene="dppB"
                     /locus_tag="OQQ66_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1903522.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6414..7316
                     /gene="dppC"
                     /locus_tag="OQQ66_000480"
     CDS             6414..7316
                     /gene="dppC"
                     /locus_tag="OQQ66_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1903523.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDIAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7327..8310
                     /gene="dppD"
                     /locus_tag="OQQ66_000481"
     CDS             7327..8310
                     /gene="dppD"
                     /locus_tag="OQQ66_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1903524.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8307..9311
                     /gene="dppF"
                     /locus_tag="OQQ66_000482"
     CDS             8307..9311
                     /gene="dppF"
                     /locus_tag="OQQ66_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1903525.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9341..10612)
                     /locus_tag="OQQ66_000483"
     CDS             complement(9341..10612)
                     /locus_tag="OQQ66_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1903526.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11088..11195
                     /locus_tag="OQQ66_000484"
     CDS             11088..11195
                     /locus_tag="OQQ66_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1903527.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11282..12961)
                     /gene="bcsG"
                     /locus_tag="OQQ66_000485"
     CDS             complement(11282..12961)
                     /gene="bcsG"
                     /locus_tag="OQQ66_000485"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1903528.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12958..13149)
                     /gene="bcsF"
                     /locus_tag="OQQ66_000486"
     CDS             complement(12958..13149)
                     /gene="bcsF"
                     /locus_tag="OQQ66_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1903529.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13146..14717)
                     /gene="bcsE"
                     /locus_tag="OQQ66_000487"
     CDS             complement(13146..14717)
                     /gene="bcsE"
                     /locus_tag="OQQ66_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1903530.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14990..15178
                     /locus_tag="OQQ66_000488"
     CDS             14990..15178
                     /locus_tag="OQQ66_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1903531.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15190..15942
                     /gene="bcsQ"
                     /locus_tag="OQQ66_000489"
     CDS             15190..15942
                     /gene="bcsQ"
                     /locus_tag="OQQ66_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1903532.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAIAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15939..18551
                     /gene="bcsA"
                     /locus_tag="OQQ66_000490"
     CDS             15939..18551
                     /gene="bcsA"
                     /locus_tag="OQQ66_000490"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS1903533.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPLDPVRYLIQTCWLLIGTSRKETPKPRRR
                     AFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLIL
                     ALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTS
                     TLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIF
                     VPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTH
                     EHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFS
                     PDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVE
                     TVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNP
                     LTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLP
                     HMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLV
                     EEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGG
                     AVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEG
                     QKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQ
                     DSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRPER
                     SETAQPSDQALAQQ"
     gene            18562..20901
                     /gene="bcsB"
                     /locus_tag="OQQ66_000491"
     CDS             18562..20901
                     /gene="bcsB"
                     /locus_tag="OQQ66_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1903534.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            20908..22014
                     /gene="bcsZ"
                     /locus_tag="OQQ66_000492"
     CDS             20908..22014
                     /gene="bcsZ"
                     /locus_tag="OQQ66_000492"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1903535.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21996..25469
                     /gene="bcsC"
                     /locus_tag="OQQ66_000493"
                     /pseudo
     CDS             21996..25469
                     /gene="bcsC"
                     /locus_tag="OQQ66_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25551..27539
                     /gene="hmsP"
                     /locus_tag="OQQ66_000494"
     CDS             25551..27539
                     /gene="hmsP"
                     /locus_tag="OQQ66_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1903536.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27722..29008
                     /gene="dctA"
                     /locus_tag="OQQ66_000495"
     CDS             27722..29008
                     /gene="dctA"
                     /locus_tag="OQQ66_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1903537.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29228..30724
                     /locus_tag="OQQ66_000496"
     CDS             29228..30724
                     /locus_tag="OQQ66_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1903538.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="OQQ66_000497"
     CDS             complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="OQQ66_000497"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1903539.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31981..32748
                     /gene="pdeH"
                     /locus_tag="OQQ66_000498"
     CDS             31981..32748
                     /gene="pdeH"
                     /locus_tag="OQQ66_000498"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1903540.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32818..34878
                     /locus_tag="OQQ66_000499"
     CDS             32818..34878
                     /locus_tag="OQQ66_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1903541.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35112..36434)
                     /locus_tag="OQQ66_000500"
     CDS             complement(35112..36434)
                     /locus_tag="OQQ66_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1903542.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="OQQ66_000501"
     CDS             complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="OQQ66_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1903543.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(37907..38806)
                     /locus_tag="OQQ66_000502"
     CDS             complement(37907..38806)
                     /locus_tag="OQQ66_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1903544.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39326..39928
                     /locus_tag="OQQ66_000503"
     CDS             39326..39928
                     /locus_tag="OQQ66_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1903545.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39979..41628)
                     /locus_tag="OQQ66_000504"
     CDS             complement(39979..41628)
                     /locus_tag="OQQ66_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1903546.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42032..43429
                     /gene="ccp"
                     /locus_tag="OQQ66_000505"
     CDS             42032..43429
                     /gene="ccp"
                     /locus_tag="OQQ66_000505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1903547.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43640..>43881
                     /locus_tag="OQQ66_000506"
     CDS             43640..>43881
                     /locus_tag="OQQ66_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1903548.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10543 a        11003 c        11920 g        10415 t
ORIGIN      
        1 tcccgttagc actaccggaa aattacccgc tgccggggat tatagcggta ttgctaccat
       61 acatgtcgaa ctggagtaac agtcatgaag cgaacaatac ttgcgttatg cctgctgcct
      121 ctttcctttt cgtcattcgc ggcaaccacg gatttagggc cgaaaggaac attgaaattc
      181 acccttaaaa tcagccaggg tgcttgcgaa ctggtgaaag acagcgttga ggttgacatg
      241 ggcaccgcgg ttctcaaaaa gccggtaaga atcggaacgg aaattaatcc cactcctttt
      301 tccatcggcc tgaaaaactg tagcgaagtg gtcagagcct acgtgactat ggacggtaca
      361 cccaatgcag acgactcaaa tctttttgcg ctggatgcgg gtggcgctac aggcgttggg
      421 ctgaaaatca aaaccgctgc tggagtacaa cagttcccgg aaaatacaac gccgataccg
      481 gttgagtttg caattaacgg tgatggcgca catcaactga actatatcgc cagctacgtg
      541 cctattcgcg ccgatgcaac tattggtcgc gcagatgcaa cggtcgactt tactgtacag
      601 tacagtacga ataatttcac atcttaaggg ccagttaact ggcccttttt actgcttttt
      661 gtaacgctaa agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc
      721 tttccccaca ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta
      781 tctgactacc tccgcacttt tcccctgccg ggcctgaaaa gccgctaagc agggtgttat
      841 cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc
      901 tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc
      961 gcttcggcag ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac
     1021 tggccgccac cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc
     1081 gcagctggcg tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca
     1141 tgaccttcct taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata
     1201 tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg
     1261 cattgcctct tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc
     1321 gaaccccagg gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt
     1381 gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg
     1441 atttgccgag ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg
     1501 cgttggggct gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta
     1561 atccggcgaa gaagtttaca taccaggctc cacaaaacgt tgatgatact tatgtcgtgt
     1621 ttatcatcgg tgaaaccacg cgttgggacc atatgggtat tttcggctat gagcgtaata
     1681 ccacgccgaa actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata
     1741 ccgcaaccaa actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc
     1801 cgcagcgcac attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt
     1861 ctgacctcta cgctatgcag agcgaaatgt ggttctacag caacacgatg gcggacaaca
     1921 ttgcttatcg tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata
     1981 tgttgctggt agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga
     2041 tcattctgca taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg
     2101 cgcagtggaa gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca
     2161 actcctatga caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg
     2221 ttcgcgataa gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac
     2281 gcgagcacct gcacggcacg ccgcgtgaat tggcaccgcc ggagcagttc cgcgtaccga
     2341 tgatggtctg gatgtcagat aaatatctgg aaaatccggc caatgcgcag gcatttgcgc
     2401 agctgaaaaa agaagccgac atgaaagtgc cacgccgtca cgtagagctg tacgatacca
     2461 tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt
     2521 gtcacatccc gcaggcaaaa gaggcagcgg ctaactaagc ggcctgctga ctttctcgcc
     2581 gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg
     2641 ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
     2701 aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcagcgag caggcttttt
     2761 tgcatttgaa gcccatgagg atgagaacct ccgggggctg ggaggttcga ctcgacgtag
     2821 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg
     2881 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     2941 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     3001 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     3061 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     3121 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     3181 caaatttctc tgctgcaaat agctgtttga agttttttaa tctttgtttg tgggttctca
     3241 ccgttggtgt aaatcccggt tggctgtatt gacgttttca cattctgttg acagattgta
     3301 ggtcacgagg ggcattttat ggaggatccg cactgttaca ctgatgttaa ttagtacggc
     3361 atccccacct cataacgttg acccgaccgg gcaaaaaaca aaaaaggtca ggtagcgaca
     3421 acccactgca aagggttaaa acaacaaaca tcacaattgg agcagaataa tgcgtatttc
     3481 cttgaaaaag tcagggatgc tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc
     3541 aagtgttcag gctaaaactc tggtttattg ctcagaaggc tctccggaag ggtttaaccc
     3601 gcagctgttt acctccggca ccacctatga cgcctcttcc gtaccgcttt ataaccgcct
     3661 ggttgaattt aaaatcggca ccactgaagt gataccgggc ctcgctgaaa agtgggaagt
     3721 cagctaagac ggtaaaatct ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa
     3781 taaagaattc aaaccgacgc gtgaactgaa cgccgatgac gtggtgttct cgttcgaccg
     3841 tcagaaaaac gcgcaaaacc cgtaccataa agtctctggc ggcagctacg aatacttcga
     3901 aggcatgggc ttgccggagc tgatcagtga agtgaaaaag gtggacgaca acacagttca
     3961 gttcgtactg actcgcccgg aagcgccgtt cctcgctgat ctggcaatgg acttcgcctc
     4021 tattctgtca aaagaatatg ctgatgcgat gatgaaagcc ggtacaccgg aaaaactgga
     4081 cctcaaccca atcggaaccg gtccgttcca gttacagcag taccaaaaag attcccgtat
     4141 ccgctacaaa gcgtttgatg gctactgggg caccaaaccg cagatcgata cgctggtctt
     4201 ctctattacc cctgatgctt ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt
     4261 gatgcagtac ccgaacccgg cagatatcgc tcgcatgaag caggataaat ccatcaacct
     4321 gatggaaatg ccggggctga acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct
     4381 ggatgacgtg aaagttcgcc aggcactgac ctacgcggtg aacaaagacg cgatcatcaa
     4441 agcggtttat cagggcgcgg gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg
     4501 ctataacgac gacgttcagg attacaccta cgatcctgaa aaagcgaaag ccttgctgaa
     4561 agaagcgggt ctggaaaaag gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc
     4621 gtataacccg aacgctcgct gcatggcgga gatgattcag gcagactggg cgaaagtggg
     4681 cgtgcaggcc aaaatcgtca cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg
     4741 cgaacatcag acggtaatga tgggctggac tggcgataac ggggacccgg ataacttctt
     4801 cgccaccctg ttcagctgcg ccgcctctga acaaggttct aactactcaa aatggtgcta
     4861 caaaccgttt gaagatctga ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt
     4921 tgaactgtac aagcaatcgc aggtggtgat gcacgatcag gctccggcac tgatcatcgc
     4981 tcactccacc gtgtttgaac cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt
     5041 aggcaaacat cacttcgaaa atgtctctat cgaataatta aaagccatac aagactgatg
     5101 gtaaaggcaa aactgcctga tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta
     5161 ttgaatttac acgattttgt aggccggata aggcgttaac gccgcatccg gcataaacaa
     5221 agcgcacttt gtcaacaatc tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat
     5281 ccggcagcaa tacttcattc tctgtccgac aagggcggga attgatttgt gagcaataca
     5341 gacacgcagt tccaggctgc gggtcactat agagaatccg ggttatgttg cagtttattc
     5401 tccgacgttt gggactcgtc atccccacgt ttatcggtat tacccttctc acatttgcct
     5461 ttgtccacat gatcccgggc gatccggtga tgatcatggc aggcgaacgt gggatctccc
     5521 ctgagcgtca cgcgcagctg ctggctgaac tcggcttaga taaaccgatg tggcagcagt
     5581 atctccatta catttggggc gtaatgcacg gcgatctagg catttcaatg aaaagccgaa
     5641 ttccagtatg ggaagagttc gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg
     5701 cgatgatttt tgctacggca gtcggtattc cggttggtgt gctggctgcg gttaaacgcg
     5761 gttccatttt cgatcacact gcagttggcc tggcgctgac cggctactcg atgccgatct
     5821 tctggtgggg catgatgctg atcatgctgg tttcggtgca ctggaacctg acgcccgcct
     5881 ccggtcgcgt gagcgatatg gtgttcctcg atgactccaa tccgttaacc ggttttatgc
     5941 taatcgacac cgccatctgg ggtgaagacg gcaactttat cgatgccgtc gcccatatga
     6001 tcttgcctgc cattgtgctg ggtactattc cgctggcggt cattgtgcgt atgacacgct
     6061 cctcgatgct ggaagtgctg ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa
     6121 cccgcatgcg ggtgattatc gtccatgcgc tgcgtaacgc gatgctgccg gtggtgaccg
     6181 ttatcggcct gcaggtggga acattgctgg cgggggcgat tctgaccgaa accatcttct
     6241 cgtggcccgg tctgggacgc tggttgattg acgcactgca acgccgcgac tatccggtag
     6301 tgcagggcgg cgtattgctg gtggcgacga tgattatcct cgtcaacttg ctggtcgatc
     6361 tgctgtacgg cgtggtgaac ccgcgtattc gtcataagaa gtaaggggac atcatgtcac
     6421 aggttactga aaataaagtg attagcgcac cggtgccgat gaccccgtta caggagttct
     6481 ggcactattt taaacgcaac aaaggcgcgg tcgtcgggct ggtttacgtc gtcatcgtgc
     6541 tgttcatcgc gatctttgcc aactggattg caccctataa cccggcggaa cagttccgcg
     6601 atgcactgct cgccccgcca gcctggcagg aaggcggcag catggcgcac ttgctgggca
     6661 ccgatgacgt aggccgtgat atgctgtcgc gcctgatgta cggtgcgcgc ctgtcgctgc
     6721 tggttggctg tctggtagtt gtgttatcgc tgattatggg cgttattctc ggcctgatcg
     6781 ccggttactt tggcggccta gtcgataaca tcattatgcg cgtggtcgat atcatgctgg
     6841 cgctgccaag tctgctgctg gcgctggtgc tggtggcaat tttcggcccg tcgattggta
     6901 acgccgcgct ggcactgacc ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg
     6961 ccgtgctggt ggaagttaac cgcgattacg tcaccgcgtc tcgcgtggcg ggtgccgggg
     7021 cgatgcgtca gatgtttatt aacatcttcc cgaactgcct tgcgccgctg attgttcagg
     7081 cgtcgctcgg tttctctaac gccattctcg atatagctgc tcttggtttc ctcggcatgg
     7141 gggcacagcc gccaacgccg gagtggggca ccatgctctc cgacgtgttg cagttcgcgc
     7201 aaagcgcctg gtgggtcgtg accttcccgg gtctggcgat cctgctgacg gtgctggcat
     7261 ttaacctgat gggtgacggt ctgcgtgacg cgctcgatcc caaactgaag cagtaagagg
     7321 ttcgagatgg cgttattaaa tgtagataaa ttatcggtgc atttcggcga cgaaagcgca
     7381 ccgttccgcg ccgtagaccg catcagctac agcgtaaaac agggcgaagt ggttgggatt
     7441 gtgggtgagt ccggctccgg taagtcggtc agttccctgg cgattatggg gctgattgat
     7501 tatccgggcc gcgtaatggc ggaaaaactg gtgtttaacg gtcaggattt gcagcgtata
     7561 tcagaaaaag agcgccgcaa cctggtgggt gccgaagtgg cgatgatctt ccaggacccg
     7621 atgaccagcc ttaacccgtg ctacaccgtg ggtttccaga tcatggaagc gattaaggtg
     7681 catcagggcg gcaacaaaag tacccgccgt cagcgggcga ttgacctgct gaatcaggtc
     7741 ggtattcccg atccggcttc gcgtctggat gtttacccgc atcagctttc cggcggcatg
     7801 agccagcgcg tgatgatcgc catggcgatt gcctgtcgac caaaactgct gattgccgat
     7861 gagccgacca ccgcgctgga cgtgaccatt caggcgcaaa tcatcgaact actgctggag
     7921 ctacagcaga aagagaacat ggcgctggtg ttgattaccc atgacctggc gctggtggcg
     7981 gaagctgcgc ataaaatcat tgtgatgtat gcaggccagg tggtggaaac gggcgatgcg
     8041 cacgccatct tccatgcgcc gcgtcacccg tatactcagg cattgctgcg tgcgctgcca
     8101 gaatttgctc aggacaaaga acgtctggcg tcgttgccag gtgtcgttcc cggcaagtac
     8161 gaccgcccga acggctgcct gcttaacccg cgctgcccct atgccactga cagatgtcgc
     8221 gctgaagaac cggcgctgaa tatgctcgct gacgggcgtc agtccaaatg ccattaccca
     8281 cttgatgatg ccgggaggcc gacactatga gtacgcaaga ggccaccctg caacaaccgc
     8341 tgttgcaggc tatcgacctg aaaaaacatt atccggtgaa gaaaggcatg ttcgcgccgg
     8401 aacgtctggt taaagcgctg gatggcgttt cgtttaacct tgaacgtggc aaaacgctgg
     8461 cagtagtggg cgaatctggc tgcggtaaat cgaccctcgg tcggttgctg acgatgattg
     8521 aaacgcccac cggtggcgag ctgtattacc aggggcagga tctgcttaag cacgatccgc
     8581 aggcgcagaa gctgcgtcgg cagaaaatcc agatcgtctt ccagaaccct tacggttcgc
     8641 tgaatccgcg taaaaaagtc gggcaaattc ttgaagagcc gctgctgatc aacaccagct
     8701 taagcaaaga acagcgtcgg gaaaaagccc tgtcgatgat ggcgaaagtc ggcctgaaaa
     8761 ccgagcacta tgaccgctat ccgcatatgt tctccggcgg tcagcgtcag cgtatcgcca
     8821 tcgcccgtgg tctgatgctc gacccggatg tggtgattgc cgatgaaccg gtttccgcgc
     8881 tggatgtttc agtgcgcgcg caggtgctga atctgatgat ggatttgcag caggagttgg
     8941 ggctgtctta tgtctttatc tcccacgacc tgtcggtggt ggagcacatt gctgatgaag
     9001 tgatggtgat gtacctgggc cgctgcgtgg agaagggaac gaaagaccaa atcttcaata
     9061 acccgcgcca tccgtacact caggcgctac tttccgcgac gccgcgcctg aacccggacg
     9121 atcgccgcga gcgcatcaag ctcagcggtg aactaccaag cccactgaat ccaccgccgg
     9181 gttgcgcctt caacgcccgc tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc
     9241 agctaaaaga ctacggcggt caactggtag cttgttttgc tgttgatcag gatgaaaatc
     9301 cgcagcgtta actcacaaaa ccggggccat gcgctccggt ttactcaatc agcttcaaca
     9361 gtggcgacaa gcatagcaaa atgccgtaaa gcagaattag ccaggcttta aatccgcgaa
     9421 gtttttccag ttgtgcgact ttatagatca ggaaaaacgg aatgaggcac gacacaatgc
     9481 catataacgg gcttcccaac tgaaagaaca ccagcaccga tacacgaaac gaaacccaaa
     9541 tcgtcaacgt aatgacgata aaagcgcaga tcgccagagt cagcatgcgt gagttaattt
     9601 tcttggtatc aataattcgg cttaacagat tgagaataat gcctttaata gcctcgtgga
     9661 aaccgagata aatgccaaag aatgcggtca gtacggcaaa gatattcagc accgtagagg
     9721 tgatatgaat gatatgtcca gggatcacct gcgcggccag tgccagtgcc gagatatttt
     9781 gttcaaaggc agaaacggct tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa
     9841 acaggatcac cgcgatgagc gtgatataac taattcggtg ggtacgcagc gcgagccggg
     9901 ttgccagtac tttatctgct tcccgtttac gataggcaat attcattggg ttaagtacct
     9961 gaataaatac cgcagaaaag aagcaaaatg gaattgtaag caggacatcg cggaaaaaga
    10021 cggaggcttg cgggaaggcg gttatattgg cgaaattcca gtgcgggatc atcgcaaaac
    10081 cgaacacgac aataatccct actttgacca ccaccattgg cccggaaatc ttaaacagta
    10141 atcgttcacc accagacgca atcgctacca gtacggcgaa aatagcgact ttataaagta
    10201 gagattgtga aagatcggca tcggttaaac cgaaggtttt caggtacgag gcgctgtcga
    10261 aaaccacgga gagagagtag ataaaaatcc cgtggataat catcaaaaag tagataaccc
    10321 cgaggaaaat tccccagttc ttccccaggt aatgactgat aatatcggtg tagtcattac
    10381 aggaatcgct ttcagaaagg gtttttaaat aaatgtcctg cactacccag gtggcaggat
    10441 aagcaatgat cgctgcggta ataaataccc aaattccctt caagccaatt tgtactggca
    10501 tcagcacggt tccggcacca atcgccatgc ctatgcataa taaaacccag ccgaagtcgt
    10561 agcgtgtaaa cggcaatttc tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct
    10621 gtccagaaag aagtcggcct cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt
    10681 ggagaagtcg aatacgcagt atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg
    10741 aggaggcgca attctacaaa attgcgcatt tcactaccag tctggagtca gcccggatat
    10801 gaactgcaac tattgcaaat aaagaaatta agctggaagg catcgcgcaa tgaggaagat
    10861 tgccgggtgc tgacagtaaa aagcaatcgt aaaatcagcg gcaagccgaa agggttccgg
    10921 tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga gagagaaaac
    10981 ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gctaatcttg aacccaaaca
    11041 actcaagaat agccgcgaaa cgttggcatt acaacacagg cggctatatg acgctcgcag
    11101 aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca
    11161 gtatgatcgt gaactggctg aataagcgga agtaacgtgt catgcgggcg tcaggctgcc
    11221 gtaatggcaa tttgcgcccg gatcaggccg cagaggttaa actctgcggc ctttttcgtg
    11281 cttactgcgg gtaaggcacc cagtcgccgc cgttcaggcg aacgtacggt ttatcctggt
    11341 attgaataac tactgcattt gagttctcgg agaccggtgc tgtttgtggc aacccactgg
    11401 tgagtttttt ccagtcaaca ttgtcttcgg tgaaaatctt gccatcaaga acgcgaacca
    11461 ccagatcgga gatagccagg aagctgctcg gttggtcgat gacaatcggt gccccctgat
    11521 gtggtgcctt catgccgaag aatttcaccc caacggggac gtctgtgata gacgggctag
    11581 ggatatcacg taggccagat acctgcattc tgtcgccctt cagcgcgccg ccgtgttccg
    11641 gcaccacgac caccatcact ttacgacccg atttctccag ttcagtaaag aaggcgtcca
    11701 gttcatcaaa gaatttctgc gcccgcgctt tgtaatccgc tgttttgctg acccccggat
    11761 aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat
    11821 ctttttcggt aacgtccagc cagcggttaa gcacggtggt atcgtcataa accggcgaac
    11881 catcaaagcc cagcaaaata accggcagat ttgtttgatc catcaattca gtctgcatgc
    11941 cgccattttc gcgaacttct ttcaaaaaac cgccgaactg gccgttatgt cccatcatca
    12001 ggtgctgggt aaagcccagt ttcgaaaggt tatcaaacag atagcagtcg ttatttgccg
    12061 gttgatacag attagtgtgc gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg
    12121 ccgggccact gtaggaggtg gcggagttaa agttcttgaa ctcaatatcg aaatgcgacc
    12181 acagtggatg cgacatcaac ccggcggctt ctatatccga ccaggaaagc gaacagatgt
    12241 taatcaccag tagttcaaat ggctgagcat cagcgggcag cgaagacggg aaggtcgatt
    12301 tacgtttcgc ctccgcgtta tagaaattat tcagccaggc gttaaggttc gccgttgttg
    12361 gcggtgtagt ttgtgcgggc atatcaccca ctaccggtgc gccacccgtc gtctcaacgg
    12421 ttgccgctgc gttaccaccc gtcgttgtta cagtggtcgt cggttgtccg gctggccaca
    12481 aggagaaact tggtcccgcc agggtaagta cgttcagcca tagcagtatg gcaaccacaa
    12541 aaacggtaat gcgaatccat tgtgacagga ataaccaggc cactaataaa acaaaaatgg
    12601 ccccaatcat ctgccagtta ataaagcgtg tgacaaggtc gattaaataa tcggtactga
    12661 accccgccac ctgcgaaccc tggctcatta tgctttccgg gccaggcaac caggtgtcat
    12721 gccagaacaa agcaaagccg atcggcaggg caatccagtg gcgcaagcga tgcaggctgt
    12781 agcgcggaat gggcatcagc agaaacgcgg caaacaccaa attgaggagc ggatggaagt
    12841 taagatatcc cgcccacaac aggccgaact taaccagaaa atagaagttc cagccggaaa
    12901 ggccgcgcca gtattgccag agggaagaag gcatggcggt attttgcgta aattgagtca
    12961 ttttttggtt gccctggctt tttccgtgcg gcgtaatgtc ccggccggtt taacataacg
    13021 aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt cgcaaagagt gccgcgccag
    13081 atagcccagc gggaaaaata tcagtgcgca aacgacaata atttcaatga tatcgctgat
    13141 ggtcatcatg atgagcgctc cacagcatca tctaacagtc gcatgggttc tggtattcgt
    13201 cgccagtggc gaccatcgtg ctcggcattg atgaccggtt tagaactttg cgttaaaggc
    13261 agcggcatgc cccattgttc tggggcaagc aggcgcatct gcaccagctc ggcactgatt
    13321 tgatcatctt caaaccagac catacggttt gagaaaatgt cgccagtagg caatgggaaa
    13381 atatgattca acgcggtatc cagatcgtta atccgacaga atgagagaaa cagcaccagc
    13441 cgattaccgc caatggtcat gatatcgccg gtacggttag ggcgacacag cgtcagggct
    13501 tgttcaacgc ggatacccgg taccggacgt agggcaacca gaacgccttt accgtgggca
    13561 ggtaatagag ggttattcat catgttgttg acggcattac agaacacatc ccacttctgg
    13621 aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg tagtgatatc ttccggcaca
    13681 tagcgactaa acttctgccc ttgcacgctt tcgatcatcg tcagacaacg ggagagtggc
    13741 gcattccacg gaataaccat atttgcaccg caggccaata acaaacgttc atcggtggcg
    13801 cgcaggctag cggtattttc ccgcacgagg attttcatcg cactaccgcg ctggcgacgc
    13861 agggtatgaa tgctgcgggc cagtggctcg atttgcgcat tttgctgtaa agaaaagacc
    13921 accgtcgccg cctgagcggt acgggcttca ttgaacagga cttcgttatt gttgaacagt
    13981 tgccagtgtt ccgatagcgg cggcgcacct tccagtacag caacattact cagaatgcgt
    14041 ttttcgtcgc tgcgtggttg gatctccgcc tcttcgcttt gaactaatgt ccagatacca
    14101 ttttgttgct gaacgctaag ctgctgacgg gcgctgaccc ctttttcgtt gcaccagaag
    14161 gcaatatcca gcaaatgttg gtcaccctga aaacgcaaac tggcaagacc aaaaagtgaa
    14221 cggtattcct caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc
    14281 aaaagcgaac aatgggttaa cctgctccat ttattcattt tatccaacca tgagcgaagc
    14341 cgctcggcag gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta
    14401 tggggatcaa tagaacactg caaatcacgg gtcaaatagt atagaccttt ttcatgattt
    14461 agcattgaaa ataattttat tttttccggc ccttgataat catctaattg aaagattttc
    14521 gccggatcgc tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt
    14581 gtttgattcg ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg
    14641 cctgctggca tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg
    14701 cccacaatgt ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag
    14761 aaataaacct accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag
    14821 gtcacaagac gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa
    14881 gtcaaaaagt gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg
    14941 ttagaattgt ctcatgaacg gtacggttat ttcataggga tcaagcaaaa tgaataacaa
    15001 tgaaccagat actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc
    15061 gttaaagcag gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca
    15121 gttggccgcg gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg
    15181 gattggtgaa tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc
    15241 atcaccgccg cattagcctg gtcattacaa atgttgggag aaaatgtcct ggtggtcgat
    15301 gcctgcccgg acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc
    15361 tgggccagag cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg
    15421 cagctcgatt tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac
    15481 aggcaaaccc ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt
    15541 taccagtgga ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg
    15601 agtttgtgcg atcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg
    15661 catcagcaag cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt
    15721 caggttcagg acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg
    15781 ctcattcatc gtgatgaagc gatagctgaa tgcctggcgg ctaagcaacc agtaggtgaa
    15841 tatcgcagtg atgcgctggc ggctgaagag atactgacgc tggcgaactg gtgcctgttg
    15901 aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg acccggtggt
    15961 tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat cgtcgtcacg
    16021 gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc tggattttta
    16081 ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac ctgtatccgc
    16141 atatcaacgc ctcgcgtccg ctggacccgg tccgttatct cattcaaaca tgctggttac
    16201 tgatcggtac atcgcgcaaa gaaacgccga aaccgcgcag gcgggcattt tcaggtctgc
    16261 agaatattcg tggacgttac catcaatgga tgaacgagct gcctgagcgc gttagccata
    16321 aaacacagca tcttgatgag aaaaaagagc tcggtcattt gagtgccggg gcgcggcggt
    16381 tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc gttactcaac
    16441 cgtttaaccc gctggcgcag tttatcttcc tgatgctgct gtggggtgta gcgctgatcg
    16501 tacggcggat gccggggcgc ttctcggcgc taatgttgat tgtgctgtcg ctgaccgttt
    16561 cttgccgtta tatctggtgg cgttacacct ctacgctgaa ctgggacgat ccggtcagcc
    16621 tggtgtgcgg gcttattctg ctcttcgctg aaacgtacgc gtggattgtg ctggtgctcg
    16681 gctacttcca ggtcgtatgg ccgctgaatc gtcagccggt gccattgccg aaagatatgt
    16741 cgctgtggcc gtcggtggat atctttgtcc cgacttacaa cgaagatctc aacgtggtga
    16801 aaaataccat ttacgcctcg ctgggtatcg actggccgaa agacaaactg aacatctgga
    16861 tcctcgatga cggcggcagg gaagagtttc gccagtttgc gcaaaacgtg ggggtgaaat
    16921 atatcgcccg caccactcat gaacatgcga aagcgggcaa catcaacaat gcgctgaaat
    16981 atgccaaagg cgagttcgta tcgattttcg actgcgacca cgtaccaacg cgatcgttcc
    17041 tgcaaatgac catgggctgg ttcctgaaag agaaacagct ggcgatgatg cagacgccgc
    17101 accacttctt ctcgccggac ccgtttgaac gcaacctggg gcgttttcgt aaaacgccga
    17161 acgaaggcac gctgttctat ggtctggtgc aggatggcaa cgatatgtgg gacgccactt
    17221 tcttctgcgg ttcctgtgca gtaattcgtc gcaaaccatt ggatgaaatt ggcggtattg
    17281 ctgtcgaaac ggtaaccgaa gatgcgcaca cgtctctgcg cctgcaccgt cgtggctaca
    17341 cctctgcata tatgcgtatt ccgcaggcgg cggggctggc gaccgaaagt ctgtcggcgc
    17401 atatcggtca gcgtattcgc tgggcacgcg ggatggtgca aatcttccgt ctcgataacc
    17461 cgcttaccgg taaagggctg aaattcgccc agcggctgtg ctacgtcaac gccatgttcc
    17521 acttcttgtc gggcattcca cgactgatct tcctgactgc gccgctggcg ttcctgctgc
    17581 ttcatgccta catcatctat gcgccagcgt tgatgatcgc cctgttcgtg ctgccgcata
    17641 tgatccatgc cagcctgacc aactcgaaga tccagggcaa atatcgccac tctttctgga
    17701 gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg gtggcgctga
    17761 ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg gaagaagagt
    17821 acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctgctcaac ctggtgggtg
    17881 ttgcggtcgg catctggcgc tacttctatg gcccgccaac cgagatgctc accgtggtcg
    17941 tcagtatggt gtgggtgttc tacaacctga ttgttcttgg cggcgcagtt gcggtatcgg
    18001 tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc gcggcaattg
    18061 cccgcgaaga tggtcacctc ttttcgtgta ccgttcagga tttctccgac ggtggtttgg
    18121 ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat ctgttgctta
    18181 aacgcggtca gcaggaatac gtcttcccga cccaggtggc gcgcgtgatg ggtaatgaag
    18241 ttgggctgaa attaatgccg ctcaccaccc agcaacatat cgattttgtg cagtgtacgt
    18301 ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat aagccgctgg
    18361 aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg gcggagtttg
    18421 cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc tgggttgtat
    18481 cgtttattct gcgccgcccg gagcggagcg aaacggcaca accatcggat caggctttgg
    18541 ctcaacaatg atgataacgc gatgaaaaga aaactattct ggatttgtgc agtggctatg
    18601 gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca accactgatc
    18661 aatgctgagc cagctgtagc cgcccagacg gaacaaaatc cgcaggtggg gcaagtgatg
    18721 ccgggcgtgc agggcgctga tgcgccagtc gtggcgcaga acggtccttc gcgtgatgtg
    18781 aagctgacct ttgcgcaaat tgcgcctccg ccgggcagca tggtgctacg tggcattaac
    18841 ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa ggcgatgctc
    18901 aacctcgaat acatgccatc gccatcgtta ctgcctgtcc agtcgcagtt aaaggtttat
    18961 ctcaatgatg agctgatggg cgtactgcca gtgaccaaag aacagttggg taaaaaaacg
    19021 ctggcgcaaa tgcccattaa cccactgttt attaccgact tcaaccgtgt gcggctggag
    19081 tttgtcggcc attatcagga cgtgtgcgaa aacccggcca gcaccacgct ttggctggat
    19141 gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg
    19201 tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac cttgccgatg
    19261 gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt cgcctcgtgg
    19321 tttggttcgc gttctggctg gcgtgggcag aacttcccgg tgctctataa ccaactgccg
    19381 gatcgtaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct gcgcgatcat
    19441 ccggcggtaa aagccccggt gattgagatg atcaaccatc cgcagaatcc ttacgtcaaa
    19501 ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct
    19561 cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa accgctgcta
    19621 ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt cacatttggc
    19681 gaactgaaaa cctatgaaga acagttacaa tccagcggtc ttgagccagc agcgattaac
    19741 gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat tgatatggat
    19801 attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga tatcagcctg
    19861 aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa ccgcctgctg
    19921 ctgcggattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc tattccggcg
    19981 ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa cccgatgccg
    20041 ggcggttcgg tggataactg tattaccttc cagccggtgc agaatcatgt ggtgattggt
    20101 gacgactcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc ggatctacgc
    20161 gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca aaccatcacc
    20221 gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac tgttggtttt
    20281 atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga tggcagcacc
    20341 attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa actgaaagac
    20401 gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac accgatgcgc
    20461 cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga aacccggtca
    20521 acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc gtataacgac
    20581 cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat gcttaacgat
    20641 gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt gatccgcgag
    20701 tccggtatca acagcctacg tgttggcgac gtttattacg taggtcatct gccgtggttc
    20761 gagcgcgtgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct ggcggctatc
    20821 agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag tcgtcgtcgt
    20881 cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat cgtgacgatg
    20941 ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg ggagcagttt
    21001 aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc
    21061 accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa cgaccgtgca
    21121 gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc tttaaaagaa
    21181 cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt gctggacagc
    21241 aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg
    21301 tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat cgcgcgggag
    21361 gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt gggttttgct
    21421 gaggataaca gctggcgttt taaccccagc tacctgccgc cgacgctggc gcagtatttc
    21481 acccgctttg gcgcgccgtg gactacgctg cgcgaaacca atcaacgttt attgctggaa
    21541 accgccccga aaggcttttc gccagactgg gtgcgttatg agaaagacaa aggctggcag
    21601 ctaaaagccg aaaaaacatt gatcagcagc tacgacgcca tccgcgttta catgtgggta
    21661 ggcatgatgc ctgacagcga tccgcagaaa gcgcggatgc tcaaccggtt taaaccgatg
    21721 gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc tacggggaaa
    21781 gcgcagggta aaggaccagt tggtttttct gccgccatgc tgcccttttt acaaaaccgc
    21841 gatgcgcagg ccgttcagcg tcagcgcgtg gccgataact ttcccggcag cgatgcctat
    21901 tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt ccgcttctcg
    21961 acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca ctaaacatat
    22021 tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca accgctcagc
    22081 aacagttgct ggagcaagtt cggttaggcg aagcgaccca tcgtgaagat ctggtgcaac
    22141 agtcgttata tcggctggaa cttattgatc cgaataaccc ggacgtcgtt gccgcccgtt
    22201 tccgttcttt gttacgttag ggcgatattg atggcgcgca aaaacagctc gatcggctgt
    22261 cgcagttagc gccgagttca aatgcgtata aatcgtcgcg gactacgatg ctactttcca
    22321 cgccggatgg tcgtcaggca ctgcaacagg cacgattgca ggcgacgacc ggtcatgcag
    22381 aagaagctgt ggcgagttac aacaaactgt tcaacggtgc gccgccggaa ggtgacattg
    22441 ctgtcgagta ctggagtacg gtggcgaaaa ttccggctcg ccgtggcgaa gcgattaatc
    22501 agttaaaacg catcaatgcg gatgcaccgg gcaatacggg cctgcaaaac aatctggcgc
    22561 tattgctgtt tagtagcgat cgccgtgacg aaggttttgc cgtcctggaa cagatggcaa
    22621 aatcgaacgc cgggcgcgaa ggggcctcta aaatctggta cgggcagatt aaagacatgc
    22681 ccgtcagtga tgccagtgtg tcggcgctga aaaaatatct ctcgatcttt agtgatggcg
    22741 atagcgtggc ggctgcgcaa tcgcaactgg cagaacagca aaaacagctg gccgatcctg
    22801 ctttccgcgc tcgtgcgcaa ggtttagcgg cggtggactc tggtatggcg ggtaaagcca
    22861 ttcccgaact acaacaggcg gtgcgggcga acccgaaaga cagtgaagct ctgggggcgc
    22921 tgggccaggc gtattctcag aaaggcgatc gcgccaatgc agtggcgaat ctggaaaaag
    22981 ccctcgcact ggacccgcac agcagcaaca acgacaaatg gaacagtctg ctgaaagtaa
    23041 accgctactg gctggcgatc cagcagggcg atgctgcgct gaaagccaat aatcctgacc
    23101 gggcagaacg cctgttccag caggcgcgta atgtcgataa caccgacagt tatgcagtgc
    23161 tggggctggg cgatgtggcg atggcgcgaa aagattatcc cgccgccgaa cgttattatc
    23221 agcagacctt gcgtatggac agcggcaaca ctaacgccgt gcgcgggctg gcaaatattt
    23281 accgccagca atcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagtcagc
    23341 ggcgtagcat tgatgatatc gaacgtagcc tgcaaaacga ccgtctggca cagcaggcag
    23401 aggcactgga aaaccagggc aaatgggcgc cggcggcagc acttcagcgg caacgactgg
    23461 cgctggaccc cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg
    23521 gacaacgcag ccaggccgat acgttaatgc gcaatctggc gcagcagaag tcgaacgacc
    23581 cggagcaggt ttacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc
    23641 tggcgcatat caacagcctg ccgcgcgcgc agtggaacag caatattcag gagctggtta
    23701 atcgactgca aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag
    23761 aagctgaagc ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc
    23821 tggctgactg ggcgcagcag cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg
    23881 tcctgacgct ggagccaact aacgccgatg ccattctggg gctgacggaa gtggatattg
    23941 ctgccggtga cacagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg
    24001 cctcgctgaa cacacagcgg cgcgtggcgc tggcgcaggc gcagcttgga gataccgcag
    24061 cggcgcagca gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg
    24121 aaagcgcgat ggtgctgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc
    24181 aggcgctgga aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc
    24241 aggataacga cacattcacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac
    24301 gcggcgtgcg cagcgatgcg gcgggcctct atcgccagca ggatcttaac gtcaccctcg
    24361 agcacgatta ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta
    24421 ccatgttgca ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg
    24481 tcaatatgaa cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg
    24541 gcacctgtac attacaggac tgtagtggca accgcagcca gtcggactcc ggtgccagcg
    24601 tggaggtcgg ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct
    24661 tcaacgtggt ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt
    24721 acaccgttaa cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa
    24781 aagactcccc gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc
    24841 taagtctgag ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc
    24901 agttaaccgg taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact
    24961 ataaggtcat taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc
    25021 attacgacaa agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg
    25081 aatacctgtc gtttgctata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg
    25141 agctgggggc gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc
    25201 tgatgaatct gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg
    25261 gcagcagtca gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca
    25321 actggtttgt tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt
    25381 tcctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc
    25441 agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct
    25501 gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa
    25561 gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt
    25621 ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta
    25681 cgcaactgga aagcattgct cgctctgtac gcgaaccctt atcttcagct attttgaaag
    25741 gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc
    25801 gtgccgatgt agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc
    25861 gcccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg
    25921 tttactcgct cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg
    25981 attccttccg tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact
    26041 tacttttgtc gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc
    26101 atccgttacg caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc
    26161 accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt
    26221 acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc
    26281 gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg
    26341 tcgcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg
    26401 cgggcgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca
    26461 aatcggtact gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca
    26521 ttgccaacgg tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacta
    26581 tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg
    26641 tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg
    26701 catttaccgc tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg
    26761 aagccgccca gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc
    26821 agtttgctat ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg
    26881 aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg
    26941 atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct
    27001 gtcgattgct tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct
    27061 ctgcgctgca actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct
    27121 atcgcattca accgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc
    27181 ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg
    27241 atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa
    27301 tcgacgtact gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga
    27361 ttgctgcaat tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg
    27421 agactgaagc acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc
    27481 tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc
    27541 taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt
    27601 tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta
    27661 ttttaaggcc gcaggtacct cataacctta caagacctgt agttttacta aaggacaccc
    27721 tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg
    27781 tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg
    27841 cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg
    27901 cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta
    27961 ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca
    28021 gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta
    28081 cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag
    28141 cgtcattggc gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt
    28201 tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg tgatcgaaag
    28261 tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc
    28321 gttcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg
    28381 gcagctgatt atctgtttct atattacctg tatcctgttt gtggtgctgg tattgggttc
    28441 aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact
    28501 gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat
    28561 ggagaaactc ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt
    28621 taaccttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac
    28681 taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc
    28741 taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc
    28801 ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc
    28861 agaagctcgt gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa
    28921 gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga
    28981 tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg
    29041 agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca
    29101 ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt
    29161 taacgtcatt tagactgact gacaacgggc agttttttat taccaggata gttgatcagg
    29221 ggttcacatg cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac
    29281 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    29341 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    29401 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    29461 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    29521 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    29581 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    29641 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    29701 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    29761 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    29821 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    29881 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    29941 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    30001 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    30061 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    30121 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag
    30181 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    30241 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    30301 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    30361 gaaactggag ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat
    30421 gggtcagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    30481 gaaactgcgg caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg
    30541 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    30601 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc
    30661 cgccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    30721 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    30781 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    30841 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    30901 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    30961 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac
    31021 taacccttcg ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    31081 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    31141 cgcgtcttca tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta
    31201 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    31261 cacttttccg ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    31321 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    31381 ttcgcaaatc gccgtagact gctcactctc cagccagaat ttggcggcgg cttcgttccg
    31441 ccagtagtag aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag
    31501 acggttttcc atccgttggg tcagggaaat atcaacgttc tcgccgtgcc aggcgtccag
    31561 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    31621 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    31681 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    31741 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    31801 ttgctggtca acaatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    31861 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    31921 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    31981 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    32041 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    32101 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    32161 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    32221 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttataga gcacggcctg
    32281 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    32341 ctgcgccaaa ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    32401 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct gggtgatttt
    32461 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    32521 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gacgcacact cttttctcag
    32581 cttttacatc taatgaatcg ctattgtcgc ggggtgattg ttgagggcgt agaaacgccg
    32641 gaagagtggc gtgatgtaca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    32701 cgcccggcac cgatagaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    32761 tccgcctggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    32821 agcaaggcag gcaaaataac tgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    32881 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    32941 gtctctgcgg agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag
    33001 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    33061 gacatcattc ttggcaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    33121 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    33181 aagctcgaaa agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg
    33241 tttaatctcg ccaacgatga taacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    33301 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    33361 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact
    33421 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg
    33481 cagggacgtt ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg
    33541 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    33601 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    33661 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    33721 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    33781 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    33841 gtctacacca ccggaaagcc gcgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    33901 cggctggcgg acctgggacc gttgattggc gttgattcgg ggaaaggggc agaaaagtcg
    33961 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    34021 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggc
    34081 cgacgtatcg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    34141 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    34201 gcgaatatcc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    34261 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    34321 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    34381 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    34441 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    34501 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtagc gcgcccgcag
    34561 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    34621 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    34681 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    34741 ccgctgattg cccgtggtgc cgttgcggcg gcactggcaa cgctggtaac accggcggcg
    34801 gcgttattgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    34861 tcgcagatga agaagtgatg aggttggatt gtgtttgctg gtgagttcgg gagcagcgtg
    34921 ttcccctcac cctaatcctc tccccaaagg ggcgaggaga ctgttttgtg cgtgttgaaa
    34981 caacgagtac cgcttttccg ttcactttct gggggagggc tgttatgttc gcgatataac
    35041 atctcgctcc gcttttctcc ctctcccttt cagggagagg gctggggtga gggtggctgc
    35101 ccacacgata cttacaacga ctgatgttgc gtctcatggg tcagcagcag ggcgattaac
    35161 gtcaagccag ccatcgccgc cagatataac cccaccgcac ctaacccgta gttagtctgc
    35221 aaccaggctg cgatatacgg cgcaacggaa gccccgagaa tcgacgatac gttgtaagag
    35281 aacgatgctc cggtgtaacg cacttctgtc gggaacagct ctggtaacag cgcgcccatc
    35341 ggaccgaagg tcagccccat cagacttaac cccagcagca ggaaggcaaa aaccagaatc
    35401 gggttgccag aaccgagcaa agggttaaag gcgaatagcg cgaacaggat gagcagcgtg
    35461 gtgatgatta ccatgctttt acggcgacca aaggcatcag ccagtaatcc agcgactggc
    35521 accatcacgc caaaaccaat aactgccatc atcaacatcc acaacacttc gttacgcggc
    35581 aggccaagcc caactggcgc ggcggcggta ctaaaggtca tggagtagac cgtcatgatg
    35641 taaaacagcg tataggttgc cagcataatg aacgtaccca gtacggttac gcgaacatgt
    35701 ttggtcagca gcgtacccag cgggatcttc acctgttttt tcgctttagc gactttctca
    35761 aacaccggcg actcatgcag cgacacgcga acatacaggc cgataatgac cagcaccgcc
    35821 gagaagataa atggcacacg ccagccccag ctcataaact gctcgtcggt cagtagccag
    35881 gaaagcagca agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc cagctgcggg
    35941 aaggagccgt acaatgcacg tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg
    36001 ccgccccatt caccacctaa gcccagaccc tgaccaaatc gagccagcgc cagcagcagc
    36061 ggggcgaaaa taccaatcgt ggcatagcct ggcagcagac caatcaccac ggtcgaaatc
    36121 cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc gccaaaatga
    36181 ccaaaaacgg cagagccaat agggcgcgcg acgaaggcga tggcgaaggt ggcgagcgac
    36241 tgtagcgttg ctgccgtagg atcgccctgc gggaagaaga tatgcggaaa cacaataacg
    36301 gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg ctgtgccaat gagagaggcg
    36361 acaaggactt tattacgcga gttgatcggc gtgtattctt gctcgtggtc gagtgttgtg
    36421 gctgttgctt gcataatctt ttcttatttt ttggctaacg aatagccata ttacgcacag
    36481 caaaagcgat atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg
    36541 ggataatgtt taggccagtg atttttcttc tatgaaatgc ttcacataat tttataaata
    36601 gtggataaaa ctggtttcag gttaaataaa agttaccaac tggatttata tgctggggtt
    36661 atgagttaga atgaaattgt ttgctcccaa atagatggct gaagaggctc tggggacgag
    36721 tttcaagagg ttatgtgcgc attatagccg gatgcgatgc tggcgcatct tatccggcct
    36781 acggggagcc aggccgaata aaatcgtatg ccggataagg cgtttacgcc gcatccggcc
    36841 tattttaagg ctgcgttttc cctggcattc gcgggtcgtc tttatattcg gcggtggcaa
    36901 tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca
    36961 gcacggaacc aaatgctgcg ccagaggggg atttcatcag cgatggcagg gtgtaggtca
    37021 tcacgatttt aatcacttca aaaccaatag cagccagaaa tgttccgcga atcagggctt
    37081 ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc agatagttgg
    37141 cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg tttcagccac tcaatgctgt
    37201 tcaggtgcag ggcgctaata atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa
    37261 gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc agatatttta
    37321 cccagaactt ctcctgatct tgcggcgagc gttcccagac atcacgcgac tgggcacgaa
    37381 tcgcttcacg caggttaccc atccagttga tgccggaata aagtgccacc gccaggccga
    37441 caagccctac agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg
    37501 ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg
    37561 gatgggaggc cagcacaaag ccccctgcgg caaacgacac catcaaaatc gggatcatcg
    37621 acaaaaatga gaaataggtg atagccgcac caaactggtt acccagccga tcgttaaagc
    37681 gttctgtcgc gcgaatcagg tgcgcaataa ccggttggcg ttggactttt tcggcagtgg
    37741 tggtgacggt ttccagcgcc tggctaactt tgccgccttt ctcgctatta tccaactgct
    37801 taatcggctc gtgctccaga tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc
    37861 gctcttttgt ccttctgtcg tggtcattct taaaaagtat agtcggtcag tccaggtgct
    37921 ctttcattac gccagccagc cactgcataa acaggtttac acgccgggaa agctcccgac
    37981 gctgcgggta aaccagcgaa agggagagcg gctcggcacg gtagccaggt aatacttcaa
    38041 taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga ataatcccca
    38101 gaccggcaag accggcggcg agatagtttt cgctactatt taccgtcaac atgccgccag
    38161 acttaaacca ctggacgcca ttaacgctgg caacctcaaa acctaacgga tgtacaccca
    38221 ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc atcggggctt tgcggataac
    38281 caaagcgcgt cagatagtgc ggactggcac agttgaccat ggtcagtttg ccgaggggac
    38341 gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat
    38401 gaagaatatc taccggacgg tcatggctac tcagttccag ctcaattccc ggatgcagat
    38461 agagaaattc cgacaggcgc ggcagtaaca ggtttttcgc gattcctggc gggatgtcga
    38521 tgcgtaattt accgctgata ctggtggcat cctgttgaaa cagaccgtcc agttcgttga
    38581 gattactcaa cacatctttt gcccgttgat aataggtcat gccttctggc gtgagtttga
    38641 cccgtcgcgt ggtgcgctgg agaagctggg tgccaagttg atgttccagt gcctgtatct
    38701 ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc tgcgcgggaa aaactttcca
    38761 gctccgcgac tttgatgaac aactgcattg cgtgaatttt atccatttct ttgaccgatt
    38821 gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt tattgttttt tctaacatct
    38881 aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga tttctcagca ggaaaagggc
    38941 ggaatagctt tatgagaaag gtgaatcccc ttaaacagag cgtatttttc agtgattccc
    39001 tttaaagtga atagaggacg tattggctat ttgtgcttat ttttgtctca ttgccatttg
    39061 ttattttggc ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta accaccaata
    39121 taaggaaatc ctcaaaattg ttttaaatag tgccctcgac atgtaatttc aggcgtttag
    39181 ctgtaactat ttatatcggt aactattttc tacgcgtcat ctctgtagaa tggccaccgc
    39241 caaatggcga attcttattt tttctcgcgg gtaataccct tccactactt aagtcttttt
    39301 aacatattca ggtggagatt cagttatgca aatagtcatg tttgacaggc agtcaatatt
    39361 tattcatgga atgaaaatca gtttacagca gcgtattcca ggggtgagta ttcagggggc
    39421 cagtcaggca gacgagttat ggcaaaagct ggaaagttac cctgaagcct tagttatgct
    39481 cgatggcgat caggatggtg agttttgcta ctggttgctg caaaaaaccg tggtgcagtt
    39541 tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat aaacggtggt tacaggaagt
    39601 tatccatttt aatgtgctgg ccattgtgcc tcgtgattca accgtcgaga catttgcgtt
    39661 ggcggtaaat agtgcggcga tggggatgat gtttctgcct ggagactggc ggacgacgcc
    39721 ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc cagcgggaga ttttaaccat
    39781 gttagcggcg ggcgagtcaa ataaggagat cggcagagcg ctgaatatca gtaccggaac
    39841 ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa gtgaaaaatc gcacccaggc
    39901 ggcaatgatg ttaaatatct cctcctgaag aggataattt tacagccagc aggaagttag
    39961 cggctggctg taaaaatatt atggttcgcc gtacaaacca attaaacggc gtaccacacc
    40021 gttagtccag ccaaacccat cctgcaacgg atactcgcca ccgccgcctt cgcgtggaac
    40081 accatcggca atatggtatt tttcgatcag tttgtgctgt tccagataga actgattcac
    40141 cgtcttcagc cagcttcgcg cgatttcatc acccagaagg tcatcgccgt acattttaaa
    40201 tccctgaatc gccatccatt gtaacggtgc ccagccgttg ggtttatccc actgttcacc
    40261 ggtttcgtac tcgcttgcca gaatcccgcc aggtgtcagt aaccgactgc gcacggcatt
    40321 tgccagacga tcggcctgtt catggttcgc cataccgaca tagagtggca caatggcggc
    40381 agcggaaaac agcgccagtt gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc
    40441 atcatcccag aggtaacgct ttaccgcatc gcgacgggca ctggccttct ggcggaacag
    40501 tgcttctgtc tctttctcgc ctttcagcgc cgagatgttg gcgatggcgc tctccagttt
    40561 aaacaggaag gcattcagat cgatggggat gaactgggtg gtacgaatac tcgccagacg
    40621 accagtatca cgcagccaac gggaagagta atcccaaccg gaggccgccc ccgctcgtaa
    40681 atcgcggtac acctcgttgg gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag
    40741 ccaggattcg tcacgcggcg tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc
    40801 cggcatccgc acaacatggc gataggcctg attagggatc aacgattctg caccgtccat
    40861 ccagaaggca tattccattt tcaggtggtc gagatagcgg cgcgcaccgc gtacaccatc
    40921 ttcttcaaac aactccacca tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata
    40981 ggtgcggttg ccgttgggga tgtgaccgta gttttcgatc atccaggcga agttatcggc
    41041 catgcatttc agcaaatctt cccgaccact ttccgccagc cccagcatgg tgaaatagga
    41101 atcccaatag taggtttcgc taaaacggcc gcccgggaca atatatgact gcggtagcgc
    41161 cagcagagaa gaccacggaa tgtgatcctg tggttcgcgg gttagcaccg gccacagctg
    41221 gtcgatatgc tctttcaggg aattttgcgg gtccgataca tactcgctgg agtagacctc
    41281 cggcagccag aagtgatttt caacaaactt gcgcaagtca aaatcacgat gacggcgcac
    41341 tttacggtag cggattaaaa tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt
    41401 tttactgtcg gggaaaattt tcgccgactg aacatgctcg aacagttcga gatagcgatc
    41461 ggcaggcgtc agcgcatcag aggcgggcag cccttcaatc atctcgggtt ccggttctgc
    41521 ctcgatcatc tcatccagtt ttaattcata cgggtccagt tcatagcaga gatcgacttc
    41581 gatcatcagt tcgtctggat tagggttttg aattttctga ttgagcataa ggatccaacc
    41641 tccgaaattc gtcgctaatt atcggctttt ctttaagggt agtcattcgc tccgcctggt
    41701 gggcgacaaa gcgtgcggtg gatcacgttt attaacttgt caaaaaatat aacgcactaa
    41761 tatcaatgga atttataaaa atacggattt ggtgttgttt ttcattttca aaacattcaa
    41821 tcctgctctc gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat
    41881 cttattgata gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg
    41941 ttttttatga tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt
    42001 cacaataact caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat
    42061 cggcctggct ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga
    42121 taataaacgc agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt
    42181 tttaggcttt ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc
    42241 cgcctattat tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata
    42301 taaatctttt aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca
    42361 aagcgatctg aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta
    42421 taccgcgcta cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg
    42481 gattgcaaaa cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa
    42541 tgaaccggtg cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg
    42601 ctttgcgctg tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg
    42661 ccatgcgttg aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc
    42721 agttgggccg attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg
    42781 ggatggtcgt gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga
    42841 aatggcgtcg aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa
    42901 agcgcagttc ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat
    42961 tgctgaattt gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg
    43021 agatgaaaat gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa
    43081 atgtgcaact tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct
    43141 gaaaaaagac tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac
    43201 taaagaagag cgtgataaat tgcgccagaa agtacccggt ttacgtaacg ttgctttaac
    43261 ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct
    43321 gcgctatcag gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct
    43381 gcacagtctg aacggggtgt acacgccgta tatgcaggat aaacaataac taatttgatc
    43441 gcccgaacag gaatgtttgg gcgattttta ttacgataat aaagtctgtt tttaatatta
    43501 tcatgttaaa tgtttatatt ataaaaagtc gtttttctgc ttaggatttt gttatttaaa
    43561 ttaagcctgt aatgccttgc ttccattgcg gataaatcct acttttttat tgccttcaaa
    43621 taaatttaag gagttcgaaa tggaccagaa gctgttaacg gatttccgct cagaactact
    43681 cgattcacgt tttggcgcaa aggccatttc tactatcgcg gagtcaaaac gatttccgct
    43741 gcacgaaatg cgcgatgatg tcgcatttca gattatcaat gatgaattat atcttgatgg
    43801 caacgctcgt cagaacctgg ccactttctg ccagacctgg gacgacgaaa acgtccataa
    43861 attgatggat ttgtcgatca a
//
LOCUS       DAJVTY010000009        43471 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVTY010000009 DAJVTY010000000
VERSION     DAJVTY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43471
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.009"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            164..421
                     /locus_tag="OQQ66_000507"
                     /pseudo
     CDS             164..421
                     /locus_tag="OQQ66_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            complement(471..1727)
                     /gene="nupG"
                     /locus_tag="OQQ66_000508"
     CDS             complement(471..1727)
                     /gene="nupG"
                     /locus_tag="OQQ66_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1903549.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1929..3008)
                     /gene="mltC"
                     /locus_tag="OQQ66_000509"
     CDS             complement(1929..3008)
                     /gene="mltC"
                     /locus_tag="OQQ66_000509"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1903550.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(3073..3348)
                     /locus_tag="OQQ66_000510"
     CDS             complement(3073..3348)
                     /locus_tag="OQQ66_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1903551.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3376..4428)
                     /gene="mutY"
                     /locus_tag="OQQ66_000511"
     CDS             complement(3376..4428)
                     /gene="mutY"
                     /locus_tag="OQQ66_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1903552.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4589..5308
                     /gene="trmB"
                     /locus_tag="OQQ66_000512"
     CDS             4589..5308
                     /gene="trmB"
                     /locus_tag="OQQ66_000512"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1903553.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5308..5634
                     /locus_tag="OQQ66_000513"
     CDS             5308..5634
                     /locus_tag="OQQ66_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1903554.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5818..6537
                     /locus_tag="OQQ66_000514"
     CDS             5818..6537
                     /locus_tag="OQQ66_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1903555.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6713..7759
                     /gene="ansB"
                     /locus_tag="OQQ66_000515"
     CDS             6713..7759
                     /gene="ansB"
                     /locus_tag="OQQ66_000515"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1903556.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7876..8883
                     /locus_tag="OQQ66_000516"
     CDS             7876..8883
                     /locus_tag="OQQ66_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1903557.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(9038..10174)
                     /gene="hemW"
                     /locus_tag="OQQ66_000517"
     CDS             complement(9038..10174)
                     /gene="hemW"
                     /locus_tag="OQQ66_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1903558.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10167..10760)
                     /locus_tag="OQQ66_000518"
     CDS             complement(10167..10760)
                     /locus_tag="OQQ66_000518"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1903559.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10768..11058)
                     /locus_tag="OQQ66_000519"
     CDS             complement(10768..11058)
                     /locus_tag="OQQ66_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1903560.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(11055..11621)
                     /gene="yggT"
                     /locus_tag="OQQ66_000520"
     CDS             complement(11055..11621)
                     /gene="yggT"
                     /locus_tag="OQQ66_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1903561.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11639..12343)
                     /gene="yggS"
                     /locus_tag="OQQ66_000521"
     CDS             complement(11639..12343)
                     /gene="yggS"
                     /locus_tag="OQQ66_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1903562.1"
                     /translation="MNDIAHNLAQVRDKILAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12361..13341
                     /locus_tag="OQQ66_000522"
     CDS             12361..13341
                     /locus_tag="OQQ66_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1903563.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13517..13933)
                     /gene="ruvX"
                     /locus_tag="OQQ66_000523"
     CDS             complement(13517..13933)
                     /gene="ruvX"
                     /locus_tag="OQQ66_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1903564.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13933..14496)
                     /locus_tag="OQQ66_000524"
     CDS             complement(13933..14496)
                     /locus_tag="OQQ66_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1903565.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14605..15555)
                     /gene="gshB"
                     /locus_tag="OQQ66_000525"
     CDS             complement(14605..15555)
                     /gene="gshB"
                     /locus_tag="OQQ66_000525"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1903566.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15568..16299)
                     /gene="rsmE"
                     /locus_tag="OQQ66_000526"
     CDS             complement(15568..16299)
                     /gene="rsmE"
                     /locus_tag="OQQ66_000526"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1903567.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16379..17086)
                     /gene="endA"
                     /locus_tag="OQQ66_000527"
     CDS             complement(16379..17086)
                     /gene="endA"
                     /locus_tag="OQQ66_000527"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1903568.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17181..17678)
                     /locus_tag="OQQ66_000528"
     CDS             complement(17181..17678)
                     /locus_tag="OQQ66_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1903569.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17755..19149)
                     /gene="galP"
                     /locus_tag="OQQ66_000529"
     CDS             complement(17755..19149)
                     /gene="galP"
                     /locus_tag="OQQ66_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1903570.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19585..20739)
                     /gene="metK"
                     /locus_tag="OQQ66_000530"
     CDS             complement(19585..20739)
                     /gene="metK"
                     /locus_tag="OQQ66_000530"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1903571.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(21043..21258)
                     /locus_tag="OQQ66_000531"
     CDS             complement(21043..21258)
                     /locus_tag="OQQ66_000531"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903572.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21394..21525
                     /gene="yqgB"
                     /locus_tag="OQQ66_000532"
     CDS             21394..21525
                     /gene="yqgB"
                     /locus_tag="OQQ66_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1903573.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21534..23510
                     /gene="speA"
                     /locus_tag="OQQ66_000533"
     CDS             21534..23510
                     /gene="speA"
                     /locus_tag="OQQ66_000533"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1903574.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23647..24567
                     /gene="speB"
                     /locus_tag="OQQ66_000534"
     CDS             23647..24567
                     /gene="speB"
                     /locus_tag="OQQ66_000534"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1903575.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24773..25531)
                     /gene="loiP"
                     /locus_tag="OQQ66_000535"
                     /gene_synonym="yggG"
     CDS             complement(24773..25531)
                     /gene="loiP"
                     /locus_tag="OQQ66_000535"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1903576.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25801..27792
                     /gene="tkt"
                     /locus_tag="OQQ66_000536"
     CDS             25801..27792
                     /gene="tkt"
                     /locus_tag="OQQ66_000536"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1903577.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            28106..28549
                     /gene="cmtB"
                     /locus_tag="OQQ66_000537"
     CDS             28106..28549
                     /gene="cmtB"
                     /locus_tag="OQQ66_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS1903578.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHR"
     gene            28577..29965
                     /gene="cmtA"
                     /locus_tag="OQQ66_000538"
     CDS             28577..29965
                     /gene="cmtA"
                     /locus_tag="OQQ66_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS1903579.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSTNAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29980..31257
                     /locus_tag="OQQ66_000539"
     CDS             29980..31257
                     /locus_tag="OQQ66_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1903580.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31254..32219
                     /gene="yggF"
                     /locus_tag="OQQ66_000540"
     CDS             31254..32219
                     /gene="yggF"
                     /locus_tag="OQQ66_000540"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1903581.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32241..32750
                     /gene="fumE"
                     /locus_tag="OQQ66_000541"
     CDS             32241..32750
                     /gene="fumE"
                     /locus_tag="OQQ66_000541"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1903582.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32747..33460
                     /locus_tag="OQQ66_000542"
     CDS             32747..33460
                     /locus_tag="OQQ66_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1903583.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33432..34109)
                     /locus_tag="OQQ66_000543"
     CDS             complement(33432..34109)
                     /locus_tag="OQQ66_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1903584.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(34103..34780)
                     /locus_tag="OQQ66_000544"
     CDS             complement(34103..34780)
                     /locus_tag="OQQ66_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1903585.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34768..35475)
                     /locus_tag="OQQ66_000545"
     CDS             complement(34768..35475)
                     /locus_tag="OQQ66_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1903586.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILVIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35476..36054)
                     /locus_tag="OQQ66_000546"
     CDS             complement(35476..36054)
                     /locus_tag="OQQ66_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1903587.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(36077..36508)
                     /locus_tag="OQQ66_000547"
     CDS             complement(36077..36508)
                     /locus_tag="OQQ66_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1903588.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36880..37899
                     /gene="epd"
                     /locus_tag="OQQ66_000548"
     CDS             36880..37899
                     /gene="epd"
                     /locus_tag="OQQ66_000548"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1903589.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37949..39112
                     /gene="pgk"
                     /locus_tag="OQQ66_000549"
     CDS             37949..39112
                     /gene="pgk"
                     /locus_tag="OQQ66_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1903590.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39327..40406
                     /gene="fbaA"
                     /locus_tag="OQQ66_000550"
     CDS             39327..40406
                     /gene="fbaA"
                     /locus_tag="OQQ66_000550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1903591.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40764..41624
                     /gene="mscS"
                     /locus_tag="OQQ66_000551"
     CDS             40764..41624
                     /gene="mscS"
                     /locus_tag="OQQ66_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1903592.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            41763..42398
                     /gene="argO"
                     /locus_tag="OQQ66_000552"
     CDS             41763..42398
                     /gene="argO"
                     /locus_tag="OQQ66_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1903593.1"
                     /translation="MFSYYFQGLDLGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            42491..43231
                     /locus_tag="OQQ66_000553"
     CDS             42491..43231
                     /locus_tag="OQQ66_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1903594.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT        10629 a        10848 c        11581 g        10413 t
ORIGIN      
        1 atctaacccg acgtacaatg tgttattgtt gttcatgctg gcctccgtgg tggaaatacc
       61 ggggtgtagc aacagctcac ccatgcggct ctgacaacga tgttaacccg cgttaaatat
      121 cccacgttgg ccagcatctg tctcacggaa agtcatactg ccttatattc gcggtgaagt
      181 ggagttggtg cggattcgtg atgccgaagg gcgaattgcg gcagaagggg cgttgcctta
      241 tccacctggc gtgctttgcg tggtacccgg ggaagtctgg ggtggggcgg ttcaacgtta
      301 tttccttgca ctggaagaag gggtaaattt gttgccggga ttttcgccgg agctgcaagg
      361 tgtttatagc gaaaccgatg cggatggcat gaaacggttg tacggttatg tgttgaagta
      421 agaataaaaa aaacgggtca ccttctggcg acccgttttt ctttgcgtaa ttagtggcta
      481 accgtctgtg tgcctgtcgg gacacgaacg tgtttatatt tgaacatcgc catgaacgcg
      541 aaggccagaa ccacggagta accagcgaaa atcaaccata cggtctgcca gtcggtaatg
      601 ccgttttggg tgtacatctc aacaacttta ccgctcacga tgccgccgag gatacagccg
      661 aagccgttag tcatcatcag gaacatccct tgtgcactgg cgcgaattgc cgggctaact
      721 tctttttcga caaacaccga accagagatg ttgaagaagt cgaatgcgca accgtaaacg
      781 atcatcgaca gtaccagcag tacagtaccg aacggagtcg ggtcgccgta agcaaacagc
      841 gcaaaacgca ggatccacgc cacaatactg atcatcatta cgttcttaat accgtagcgg
      901 cttaagaaga acgggatggt cagaatgaac agggtttcag agatctgcga aatcgacatg
      961 atgattgacg catgctgcac aataaagctg ctggcaaaca tcggatcttt gtcgaagctg
     1021 tgcaggaagg tattaccgaa catgttggta atctgcagtt ccgcgcccag cagcattgag
     1081 aagataaaga agattgccat acgcttgttt ttaaacagcg cgaatgcatc gaggcccagc
     1141 agggttgtcc agctctgatt cgcttgctgt ttagcaaccg gaatatgcgg cagagtcagg
     1201 gtaaacagaa ccagaatggc ggaaagtgct gcgccaatat acagctgcat gtggcttaat
     1261 tcgaagccag acaggctcac cacccacatt gccatgataa agccgatggt gccccagata
     1321 cggattggcg ggaagtcagt aacgatatcc atcccggcat tttgcaggcg atagtaagag
     1381 atggtgttga ttaacccaag cgttggcata taagcaaacg agttaatcaa tatcacaagg
     1441 aacatcgcct ccggtgtcgt gacctgtgcc gccatgaaca gcgtgatagc gccaatggtg
     1501 tggcaaatgg catataccca tttcgcactt aaccatttgt cggccacaat ccccagcagc
     1561 gcaggcataa agaccgctgc gatacccagt gagctataaa ctgcgccaat agaagcaccg
     1621 tcaaacttca gggtaacaaa catataggag ccgagggtcg tcagccaact tccccacaga
     1681 cagaactgca gaaaagagag gattttcagc tgcagcttaa gattcatgtt aatttcctca
     1741 catcgtgatg cggatgagtg tttgaaaaag cacatttgac cgtgcgagat gatgggattc
     1801 tatcaacttc gcggactggt tttttgttac ctgtggcaaa taattgcaaa cattcccaat
     1861 ttgcaaaacg aaattgtgat gtggataaca tttttgcccc tgagcatcgt caggggcggt
     1921 taatggaatt atcggcggcg gtaggatttt tgcgcggtat tcactttata aagataacgg
     1981 cgagattccg cagagggatg gcgggtcgtc agcgtctgat aaacatcgcc cggcgtcatg
     2041 gtgttaataa tattggcagc ctgaatctta tcattcgaaa agactcgcag cacgctgcct
     2101 gcgccgccgt tataggcggt gatgacggca taacgccgcg atgttgggtt atcaattccg
     2161 ccgagataaa cattgttcag catcgccaga tacgcggtgc cggtatcaat attgctggca
     2221 ggatcaaaca agaaactgcg gctcggcgtg ccggatttcc cctgcgagcg gaacacatct
     2281 ttcccggcag tatgttgtac cacctgcatt aatcccagcg catcggaacg gctgaccgca
     2341 tacgggttaa aggaagattc ggtctgcata attgccagaa tcagcgactc atcaacgcca
     2401 tatttccgtg acgcctggcg gaccatgccg agatatttgt gcgcacgttt atcaaggtgg
     2461 ttcggtacca tgttaatggt gacgctgtag atgatacgca gcccgttact gcggctttgc
     2521 agacggtttt tcagcagata atccgcgaag ttgcttgcgc gaccttccca gcgaatcggc
     2581 tgcccggtgt tgtccaccac ctgaccgtaa aggaaaggtt ctttcgaaat cgtaatatca
     2641 tcaacgtcgg aatagagatc gaccgaactc ggatcgtcac ccatcagcaa cgttttgata
     2701 attgcccggc gcaaatgcgc ggcaggttct gtcccggcga tagtttcgat agtaatcgta
     2761 ccgtcatcga agttgatgtg gctgcgggtc tgatattgat cggtgtattt cacgtagtcc
     2821 ttaggaccgg cgatcaccac ctctttgaat ccccagatgt tctcaatatt gtgggcaaat
     2881 tgccccatca gaatatcaaa accgttggta tctttgaccc aggcttcgtt ataggtatcg
     2941 ccttttttgg tcgtcgaaca ggagatgagc aacggcgcaa tcaaagccag cgcgagatat
     3001 tttttcatca ttccgggtgc gtgttgtgtt tgtttttatg caagttgccg gaggcgtgct
     3061 ccggcactgt ttttattttt tatcttccgg cgtatagccc tcgatatgca cctctttacc
     3121 ctcgaacagg aagttgacca tctcctgctc aagcagcttg cggtgctcgg cattcatcat
     3181 gttgagtttc ttttcattaa tcagcatggt ttgcttgtgc tgccactgcg cccaggcttc
     3241 tttggagatc tcgttataga tgcgttttcc cagctcgccg gggtacagct gaaaatcctg
     3301 accttctgct tcacgttgca ggaaagtaca aaaaatcgtt ctgctcataa atcatcctct
     3361 ttatcgactc acgcgctaaa ccggcgcgcc agtgcgtaac tgctgtaaca aacgctccac
     3421 gggagccgcc aggccaactg acggcggttg cgctaagtta taccagagcg cattgccttc
     3481 atccatgcag ccggtgaatg acgacacggg aagccacata ggcacaatat ctaagtggaa
     3541 atggctgaag gtatgccgaa acgcggtcag ttgcgtcagg ttatcggcag caatctgccg
     3601 ttgcgccagc cactgccgca aactttcttc gtcggcaaac tgcgggaaac agtataaacc
     3661 gccccacaat ccgctcggcg gacgctgcgc cagcaatact tcatcttcgt gctgtaacag
     3721 caaaaagtag ccggtgcgct ccggcagcgt ctgtttcggt tttttgcccg gataaagcga
     3781 ccagctatta ttggcggcgg caatacatcc gttttgtagc ggacagagcg aacatttcgg
     3841 cttcgagcgc gtacaaatca tcgcgcccaa atccatcatc gcctgattaa accgttccac
     3901 gccgaccgcg ggcgtcacct gctcgctcaa actccataat ttattctcga cctctttttt
     3961 cccaggccag ccgcttacag catagcagcg cgccagcacg cgtttgacgt taccgtcgag
     4021 aatcggaaag tgcttaccca gagaaagcga gagaatcgcg cctgcggtgg aacgcccgac
     4081 gcccggtaac gccgcgactt cttcaaaggt ttccgggaat ttaccgccgt gtaaggtcgc
     4141 cacttgttgt gccgctttat gcagattgcg cgcgcgggcg taatagccaa gcccggtcca
     4201 caagtggaga acttcgtcga gcggcgcatt ggcgagatcg gtcaccgtcg ggaagcgcgc
     4261 cataaagcgt tcaaaatagg ggataacggt cgcaacctga gtttgttgca acatcacttc
     4321 tgagagccat actttgtagg gcgtcttgtc aatttgccag ggcagcgttt ttcgcccgta
     4381 tttatcgtac cagtccagaa cctgggctga aaattgcgac gcttgcatgg tcaccgaatt
     4441 cactgttgtt gggggcaaga ttgcagcaca gcgacggcag ggtgtaaacc ggaactttcc
     4501 gcagccacgg ccttatcatg cttgcatccg gcaactaact ttggataatg cccgttttca
     4561 gaacactttc acaagcgact aaatctttat gaaaaacgac gtcatttcac cggaatttga
     4621 tgaaaacggc cgcccactgc gccgtatccg tagttttgtg cgccgccagg ggcgactgac
     4681 caaaggccag gaacatgcgc tggaaaacta ctggccggtg atgggcgttg agttcagcga
     4741 agatatgctg gatttccccg cgctttttgg ccgtgaagcg ccggtgacgc ttgagattgg
     4801 ttttggcatg ggggcgtcgc tggtggcaat ggctaaagat cgccctgagc aggacttcct
     4861 cggcattgaa gtgcattcac cgggcgttgg tgcgtgcctg gcttctgcgc atgaagaagg
     4921 tttaaacaac ctgcgagtga tgtgtcacga tgcggttgaa gtgctgcata aaatgattcc
     4981 tgacaattca ttgcgcatgg tgcagctctt tttccctgac ccgtggcaca aagcgcgcca
     5041 taataaacgc cgtatcgtac aggtgccgtt tgccgaactg gtaaaaagca aactgcagct
     5101 ggggggcgta ttccatatgg cgaccgactg ggaaccttat gcggaacata tgcttgaagt
     5161 gatgtcttct attgacggtt ataaaaacct gtcagagagc aatgattacg taccgcgtcc
     5221 ggcatcacgt ccggtgacga aatttgaaca acgtggtcat cgtcttggtc acggagtatg
     5281 ggacttaatg ttcgagaggg tgaaataatg gcaaagaacc gtagccgtcg tctgcgtaaa
     5341 aaaatgcaca tcgacgaatt ccaggaatta ggattttcgg tggcatggcg attcccggaa
     5401 ggtacatcgg aagaacagat tgataaaacc gttgatgatt ttattaacga ggttatcgaa
     5461 ccgaacaaac tggcctttga cggcagcggt tatctggcct gggaaggtct gatctgcatg
     5521 caggaaatcg gcaaatgcac cgaagaacat caggcgattg tgcgtaagtg gctggaagag
     5581 cgcaaactgg atgaggtacg caccagcgaa cttttcgacg tttggtggga ctaagaaagc
     5641 atacgggcga tgacaaatgc aaaactgcct gatgcgctac gcttatcagg cctggaaaga
     5701 tgcacgatcg agtaggcggg ataaggtgtt tacgccgcat ccggcatgga aaacgcgtac
     5761 tttgttatca atctggggcc agcaaatgct ggcctgattt tttcttgagg gaagactatg
     5821 atgcgcaaaa tgctgctggc ggcagcactt tcagtgacgg caatgaccgc tcacgccgac
     5881 taccagtgca gcgtcacgcc gcgtgacgat gtgattgtca gcccgcaaac cgtgcaggtg
     5941 aagggcgaaa acggcaatct ggtgatcacg ccagacggca acgtgatgta taacggtaag
     6001 caatattccc tgaatgccgc ccagcgcgag caggcgaagg attatcaggc tgaactacgc
     6061 agcacgctgc cgtggattga tgaaggcgcg aaaagccgcg tcgaaaaagc ccgtattgcg
     6121 ctggataaaa ttatcgttca ggagatgggc gaaagcagca aaatgcgcag ccgtctgacc
     6181 aaacttgatg cgcaactgaa agagcagatg aaccgcatta tcgaaacgcg cagcgatggc
     6241 ctgacgtttc actataaagc cattgatcag gttcgcgccg aaggccagca attagtgaat
     6301 caggcaatgg gtggaatttt acaggacagc attaatgaaa tgggcgcgaa agcggtgctg
     6361 aaaagcggcg gtaacccatt acagaatgtg ctgggaagcc tgggggggct gcaatcctca
     6421 atccaaaccg agtggaaaaa gcaggaaaaa gatttccagc agtttggcaa agatgtttgt
     6481 agccgcgttg tgactctgga agatagccgc aaagccctgg tcgggaattt aaaataatcc
     6541 tctattttaa gacggcataa tactttttta tgccgtttaa ttcttcgttt tgttacctgc
     6601 ctctaacttt gtagagctcc aaaatatatt cacgttgtaa attgtttaac gtcaaatttc
     6661 ccatacagag ctaagggata atgcgtagcg ttcacgtaac tggaggaatg aaatggagtt
     6721 tttcaaaaag acggcacttg ccgcactggt tatgggtttt agtggtgcag cattggcatt
     6781 acccaatatc accattttag caaccggcgg gaccattgcc ggtggtggtg actccgcaac
     6841 caaatctaac tacacagcgg gtaaagttgg cgtagaaaat ctggttaatg cggtgccgca
     6901 actaaaagac attgcgaacg ttaaaggcga gcaggtagtg aatatcggct cccaggacat
     6961 gaacgataat gtctggctga cactggcgaa aaaaattaac accgactgcg ataaaaccga
     7021 cggcttcgtc attacccacg gtaccgacac gatggaagaa accgcttact tcctcgacct
     7081 gacggtgaaa tgcgacaaac cggtggtgat ggtcggcgca atgcgcccgt ccacgtccat
     7141 gagcgcagac ggtccattca acctgtataa cgcggtagtg accgcagctg ataaagcatc
     7201 cgctaatcgt ggcgtgctgg tggtgatgaa cgacaccgta ctggacggtc gcgatgtaac
     7261 caaaaccaac accaccgacg tagcgacctt caagtctgtt aactacggtc ctctgggata
     7321 cattcacaac ggtaagattg actaccaacg taccccggca cgtaagcaca ccagcgatac
     7381 gccattcgat gtctctaagc tgaatgagct gccgaaagtc ggcatcgttt ataactacgc
     7441 taacgcatcc gatcttccgg ctaaagcact ggtagatgcg ggctatgatg gcatcgttag
     7501 cgctggtgtg ggtaatggta acctgtataa atccgtgttc gacaccctgg caaccgccgc
     7561 gaaaaacggc actgcagtag tgcgttcttc ccgcgtaccg acgggtgcta ccactcagga
     7621 tgctgaagtg gatgatgcga aatacggctt cgtcgcctct ggcacgctga acccgcaaaa
     7681 agcgcgcgtc ctgctgcagc tggctctgac gcaaaccaaa gatccgcagc agatccagca
     7741 gatcttcaat cagtactaat cacctcgctc cggtatcgtg ccggggcttt ttcacttcag
     7801 acttacgtcc attgccaatt ttcattaccc taatgataat caccggaata aattatttcg
     7861 cgtgaggttt ttcgggtgaa aaagcaatgg attgttggca cggcactgct tatgttgatg
     7921 actggtaatg cctgggcaga tggcgaaccg ccaactgaaa atatcttaaa agatcaattc
     7981 aaaaagcagt atcacggcat tctcaagctt gatgctatca ccttaaaaaa tcttgatgct
     8041 aagggtaatc aggccacctg gtcagcggaa ggcgatgtct cttccagtga cgatctctat
     8101 acctgggtcg gtcagttggc agattacgaa ctgctcgaac agacctggac gaaagataaa
     8161 ccggtaaaat tctcggcgat gttaaccagt aaaggaacgc ccgcgtctgg ctggtcggtg
     8221 aacttttact cttttcagtc ggcagccagc gatcgtgggc gggtggtcga cgatatcaaa
     8281 acgaataata aatatctgat cgtgaatagc gaagatttca attatcgctt tagtcagctt
     8341 gaatctgcgt tgaataccca gaagaactcg attcctgcgc tggaaaagga agtgaaagcg
     8401 cttgataagc aaatggtggc ggcgcagaaa gcagcggatg catattgggg aaaagatgca
     8461 aacggtaagc aaatgacccg cgaagaagcc tttaaaaaaa ttcaccaaca gcgtgatgag
     8521 tttaataaac agaacgatag cgaggcgttc gcggttaaat atgacaaaga ggtttatcaa
     8581 ccggcgattg cggcatgtca taaacagagt gaagagtgtt atgaagtgcc gattcagcag
     8641 aaacgagatt tcgatatcaa cgaacaacgg cgacagactt ttctgcaatc acaaaaactc
     8701 agccgtaaat tgcaggatga ctgggtaacg ctcgaaaaag ggcaatatcc gttaacgatg
     8761 aaagtttcag aaataaacag taaaaaagtc gcgattttga tgaaaattga cgatatcaac
     8821 caagctaatg aacgctggaa aaaagatacc gaacaactgc ggcgcaatgg ggtgattaag
     8881 taagtttgat atagtgcacg atttgtagca ggatgcggcg ttgaccccgc atccggcaat
     8941 caacgtctga tgcgacgctt gcgcgtctta tcagacctac aaccgttgcc acatgtaggc
     9001 tggataaggc gttcacgccg catccggcaa tacaagttta ctcagccaga aaaagctcca
     9061 gcagcgaatt taaaaacagc ttcccatgtt ccgttatctg ccagtaatcc gcacattctg
     9121 tgagataacc ctgggcaatc gcctcgtcta actgtgggcg aatcacatct tcgcaaagcc
     9181 cggtatacgc actaaactct gcgcgtggcg cggcctccag caagcgaaag cggttcataa
     9241 agaactcaaa cggcttatct gcggcttcga catcacgctg gctttccaga taccttcctt
     9301 gcataaaacc acgaggatga cgcgttttag tggtacgcag aatgcgccca tccgggaagg
     9361 tgactttgcc gtgcgcgccg cagccaatac cgatgtagtc accaaatcgc cagtagttga
     9421 gattgtgctg gcactgataa ccgggtttgg cgtaagcgga agtttcatat tgctgataac
     9481 ccgctgcggt taataactga tgcccctgtt cgaaaatatc ccacagcgcg tcatcgtccg
     9541 gcagcaccgg tggtcgcgaa ccaaacagcg tattgggttc gatggtcagt tgataccagg
     9601 aaagatgcgg cggattcagt tcaatggcct ggcgtagatc gccaagcgcc tcttccagtg
     9661 attgatccgg tagtccatgc atcaaatcaa ggttaaagct acgtaaccct aaaccgctcg
     9721 ccagctttgc cgctcgtttc gcttcttgcg ggccatgaat gcgcccaagt cgtttcagct
     9781 tttcttcgct aaaactctgc acaccaatag agatgcggtt cacaccagca cgctgataat
     9841 cgacaaagcg atcggcttct accgtaccag ggttcgcttc catagtaatt tctgcatccg
     9901 ctgccagcgg caaacgcgca cgcacgccgt ccagcagcgt ttgcatcgcc gggccggaaa
     9961 gcaggctcgg cgtaccaccg ccaataaaga ttgtctttac ttcacggccc tgagcgtaag
    10021 ccacatcatt gtccagatcg caaagcagat gctgaacata atcgtcgtgc ggcacttctc
    10081 ctttcaacgc gtgagagttg aaatcgcagt acgggcattt ctgcacgcac cacgggatgt
    10141 gaatgtagag actcagcggc ggtaatttaa ccattacgta aagcgtccag cagcagtttc
    10201 aacgcctgac cacggtggga aatggcgctc ttttcttcgc gggtcagttc ggcggcggtt
    10261 ttcccttcgg aaggtacgaa gaagattgga tcataaccaa agccaccagt gcccgctggt
    10321 tcgcgagtaa tcacgcccgg ccagctgccg tggcatacca gcggagtcgg atcttccgcg
    10381 tgacgcagat ataccagcac gcagtggaaa cgtgcctgac gttggtcgtc cggtacgtct
    10441 ttcagtgttt ccaacagttt ttgcagattc ttttgatcgg tcgcgtcttc accggaataa
    10501 cgcgcggagt aaatacccgg cgcgccaccc agcgcatcta ccgccagacc agagtcgtcg
    10561 gcaattgccg gtaaaccggt cacttttgcc gcatggcgcg ctttcagaat cgcgttttcg
    10621 ataaaggtta ggccggtttc ttcagcggaa tcaacgccga ggtctgtttg tgccacgata
    10681 tcaagaccaa agtcgctaag cagcgacgcc agctcacgca ctttaccggc attgccggtt
    10741 gcgaggacaa ctttttgcat aggataccta attaattaac gccgcgattt ctggcgggat
    10801 ttgttgcgga ttaatgattt taatttgttt gtggcggcca agttcgcctt tttcaatcac
    10861 cacctggctt ttggcaaccc ggaattgctt accgagaaac ttcaccagat gactgttggc
    10921 ctggccgtca actggcggcg cggtaatggc gactttaact tcgtcgccat gtaaaccgac
    10981 aatagaatca cggctggctt tcggctgaat atagagccgt aaaaccagac cgtcatcatt
    11041 aactgttacg gcattcataa cgccatccac atccccggca gcaggatgtt tccggttgcc
    11101 tgtaatactt ctgcgacacc catattgatg acatacagca gcagaacgag gatcatcggc
    11161 gagaaatcaa tcccacccat tgccggtagc aagcggcgaa tcgggcgcag cagcggatcg
    11221 gccagctgaa tcagcacgta ttcaatcggg ctacgaccct ggcttaccca gctcataatc
    11281 gccatcacca gcaggaccca gaaaatcagc aagccgatgg ttttcagcag aatcagtaaa
    11341 ccggcaatcc agatgattgg caggaaggtc accactttaa acagcacaat ggctttgata
    11401 aaactgagaa tataggcaac cagcagcgag gcgctgtcaa tgggacccat tgccggaata
    11461 acgcggcgca gtggcccgat aattggctgc gttaccttca ctacaaactg tgagaagggg
    11521 ttgtaaaaat cacaatgagc ccactgcatc cagatgcgta ataacagcac catggtatac
    11581 agctcaatga ccgttgaaag caggaaagtc aacgtattca tggcgttcct ttaattcctt
    11641 attttttaga gtaatcacgc gcaccaaaaa ttgcagtgcc gatacgaacc atcgtgctac
    11701 ctgccgcaat ggcggcttcc atatcgtccg acattcccag agagagcgtg tcgatatgcg
    11761 ggtagcgcgt tttcagtccg gcaaatgcta cagccatttg gcgtgcaact tcaaactgcc
    11821 ttacatattc tgactcaggc gcagggattg ccatcagccc gcgcagacgt aaacgcggta
    11881 gttcagcgac cgccgctgcc agctcgtcca gttctgccag ttgaatcccg gacttactgt
    11941 tttcatcact aatattaatt tgaatcagta cgttaagagg gggaagttct gccgggcgct
    12001 gatcgttgag gcgggtagca atgcgcaaac ggtcgatggt atgacaccag tcgaaatgct
    12061 ctgccaccag gcggctttta ttagactgca acgggccaat aaaatgccat tctaatcctg
    12121 ttacgcccag ttcctgaaag tggcgaattt tatctacccc ttcctgaacg tagttttcac
    12181 caaattgacg ctgcccggca tcaatggctt ctgcgatggc gctcgcaggt tttgttttac
    12241 tgactgcaag cagcgtaatt tcttctggag aacggccgca acgcgttgca gccgctaaga
    12301 ttttgtcccg gacctgtgcc aggttatgcg caatatcgtt cattttccga ggatcttagt
    12361 atgaatatgg aagaaattgt ggcccttagt gtaaagcata acgtctcgga tctacacctg
    12421 tgcagcgcct ggcccgcacg atggcgcatt cgcgggctaa tggaagctgc gccgtttgat
    12481 gcgctggacg tcgaagagct actgcgggag tggctggatg acgatcagcg ggcaatattg
    12541 ctggaaaatg gccagctgga ttttgccgtg tcgctggcgg aaaaccagcg attgcgtggc
    12601 agcgcattcg cgcaacggca tggcatttcg ctggcgttac ggctgttacc ttcgcactgc
    12661 ccgcagctcg aacagcttgg cgcaccaacg gtattgccgg aattactcaa gagcgagaat
    12721 ggcctgattc tggtgacggg ggcgacgggg agcggcaaat ctaccacgct ggcggcgatg
    12781 gttggctatc tcaatcaaca tgccgatgcg catattctga cgctggaaga tcctgttgaa
    12841 tatatctatg ccagccagcg atgtttgatc cagcagcggg aaattggttt gcactgtatg
    12901 acgttcgcat cgggattgcg ggccgcattg cgggaagatc ctgatgtgat tttgctcgga
    12961 gagctgcgtg acagcgagac aatccgtctg gcactgacgg cggcagaaac cgggcatttg
    13021 gtgctagcaa cattacatac gcgtggtgcc gcgcaggcag ttgagcgact ggtggattca
    13081 tttccggcgc aggaaaaaga tcccgtacgt aatcaactgg caggtagttt acgggccgtg
    13141 ttgtcacaaa agctggaagt ggataaacag gaaggacgcg tggcgctgtt tgaattattg
    13201 attaacactc ccgcggtggg gaatttgatt cgcgaaggga aaacccacca gttaccgcat
    13261 gttattcaaa ccgggcagca ggtggggatg ataacgtttc agcagagtta tcagcagcgg
    13321 gtgggggaag ggcgtttgtg acgttgccat gcccgatgcg caagtttata gggcctataa
    13381 aggcaataaa tgtctgatgc gacgctaacg cgtcttatca gacctacagc cgctgccaga
    13441 tgtgtgctgg ataaggcgtt cacgccgcat ccggcaagat ggcggtaacg actatccgcc
    13501 aggcttttag atcgccttaa tatccctgct cgaaatagct ttcgagaata ataaccgcag
    13561 aggcagagtc gactttgcct ttgttgagcg cccgatagcc gccctgttca aacagaccgg
    13621 aacgggcttc cacggtgcta agacgttcgt catggagctt tacttcaaca ccgaaacgac
    13681 catgaatacg gttggcaaat ttacgcgctc tggcagtcaa tggttgctcg gtgccgtcca
    13741 tattcagcgg caaaccgacg atgatttcgt ccggctgcca ctctttcagt aaacgttcga
    13801 tgaggttcca gtccggcgta ccgtcctgtg ctttaattgc aggcaaaggg cgagcagtgc
    13861 cggtaatgcg ttggccgacc gctacgccaa tgcttttggt gccgaagtcg aaggcgagta
    13921 aggttccact catcaggcgt gccctgccac accaggcatg gtgagaatat ccacaccaat
    13981 cagttttgcc gcctcgcgcc agcggtcggc aatcggcgtt ttgaacagaa tattcagatc
    14041 cgccgaggcc gttagccacg cgttatcgag aatttcttgt tccagttgac ctttttccca
    14101 ggaggcatag cccagagcca ccaatacgtc agacggttgt ttatcggtgc cgagcgtttc
    14161 cagcacatcg cgggaagtgg tcatcaccgt gttgtctgaa atgcgaatgc tggaagcaaa
    14221 attggagggc ggagtatgca aaataaaccc gcgatcttca gccagtgggc cgccgagcat
    14281 aaccggttta tccagacgga ttgattcatc acgcggctcc ggcgtgatct tcagcttttc
    14341 cagaatccct tcaattttga gattttccag cggcttgttg acgatgatcc ccattgcacc
    14401 attggtatta tgttcgcaaa tgtagaccac ggaacgacgg aaaatcggat cctggagagc
    14461 aggcatggca ataagaaagt gatgctgtaa attcattgtc agaggttctg tttcctggtt
    14521 caaaaaagca acagcgccca gtatgcgtgg aaagaagggc gctgtcactc agagtctcaa
    14581 cgagatcctt ctcgctaagg tgggttactg ctgctgtaaa cgtgcttcga tggcatccat
    14641 taacattccg gtgatcgaca ccggaaactc tgcttcaatc tcacgaatac aggttgggct
    14701 ggtgacgtta atttcagtca gacggtcgcc gatgatatcc agaccaacaa aaatcagccc
    14761 tttttctttc agcgtcggcc cgatctgacg ggcgattttc cagtcacttt ccgtcagcgg
    14821 ccgaggttca ccgcgaccac cggcagccag attgccacgg gtttcgcccc cctgcggaat
    14881 acgcgccagg cagtacggta ccggctcgcc atccaccacc agcacgcgtt tgtcgccatc
    14941 tttaatggct ggcaggtaat tttgcgccat gcagtagcga gtgccatgct cagtcagggt
    15001 ttcggcaatc acgccgaggt ttggatcgcc ttctttcacg cggaaaatcg acgcgccgcc
    15061 cataccgtcc agcggcttaa gaatgatgtc gctgtgtttc tcccagaacg cttttagctg
    15121 cgctttattg cgcgtaacca gcgtttctgg cgttaagtca gagaaccagg cggtaaacag
    15181 tttctcgtta cagtcgcgca ggctctgcgg cttgttaacg atcagcgtcc ctttctcttc
    15241 ggcacgttcc agaatatagg tcgcgtagat aaactcggta tcaaacggcg ggtctttacg
    15301 catcaggagc acatcgagat cggccagcgg cagatcctgt tcaccgacga acgaaaacca
    15361 ctcttcgtag ttctgcttca cgttcagcgt gcgggtatgg gcgcgagctt cgccattgat
    15421 cagatacaga tcgcccatct ccatatagtg aagttcgtaa ccacgacgct gtgcttccag
    15481 caacatagcg aaactggaat ctttcttgat gttgatgttt gcgatggggt ccatcacgat
    15541 gccgagcttg atcattattc ttctccgtta gcccaaatcg ccaaatcgta cttgtagcgc
    15601 ggtaatggcg gtgagcgcag ttgtctctgt acgcaaaacg cgaggtccca acaggatatc
    15661 agtaaattga tagcgggcag tcatggcaat ttcatctgcc gataaaccgc cttccgggcc
    15721 aatcagcagg cggacgcgtt caaccggtaa cggcaacgta ttgatgctgt tactggcgcg
    15781 cgggtgaaga ttcagtttca gtccttcatc ctgctctgca caccaggctt ccagatccat
    15841 cgctggacgg atttccggca cccggttacg accacactgc tcacaggcag caattgcaat
    15901 cttctgccac tgctggagct tcttgttcag acgttcacta tccagtttaa cgccgcagcg
    15961 ctcagaaaaa agtggcgtaa tgaggcttac accgagttcg atcgatttct ggatcgtaaa
    16021 ttccattttt tcaccacgcg acatcacctg accgaggtga atatgcagcg gagattcgcg
    16081 atcgtcgatc ttgccttcca gcaccttcac ttccacgctt tttttgctgg cgctggtaat
    16141 ttcggcgtca aagacctggt tgctaccgtc aaacaattgc aacgcctgcc ccggccccat
    16201 gcgcagtacg cgcccgatat ggttggcggc atcttcgcaa agcgcgatgt gagaatggct
    16261 ggtcagtggt tcaggatgat aaatgcgggg gatacgcata gttaaaaatc cgcgtcgtct
    16321 ccccacgcgg ttgtacgcgt ggggtagggg ttaacaaaaa gagtcccgct agtgtaggtt
    16381 agctctttcg cgcctggcaa gcgcgttgca catacgggtt atgattgcct tgcaccttcg
    16441 cgatgcgttc atcgcgctcg cactcccagt cggtaaccgg atacatcttg ttccatgcgt
    16501 tgaacagctg cgtttgctgg cgagagagtg tcaggttgta ttggtcgcgc atatagaagt
    16561 aggtgcgcgc aatggcaccg cgtgcacgcg ctggtggttc ggcagctttt tctttgaaat
    16621 cgaccttcat ggcgcattga ccgtactggc cttcgccgcc attccactgg ctgtacataa
    16681 agttgccgcg atcgccattc acctcaccga ctgacggctg caggttatgc atatcgcttt
    16741 ccatcttgcg atagaccgga tctttagcgc agtttttacg tccaccgtcc tgccagcact
    16801 ggcgctggtg accgaactgc caggcgggaa cgacgtgttc ccactctacg cggctggctc
    16861 ggttttcatt tttgcgcacc tgatagccgc acgattgcag atcaacaacg ccttttttgc
    16921 cctgccagtt aattttacat ccgcaataaa acgtaccggg cgcgtcagcg tggactttta
    16981 ccgccgcggc tttcgcctga gaaaaactat tgataccttc ggccaacgcc gggccggaaa
    17041 atgctgcgct cagtaccacc gcagcaatag acaaataacg gtacatcact tactccgtgt
    17101 taaaacgagc cagcaacgta gcgaaagttg ttttggtatg caatcagcca gatcaggaaa
    17161 gttcctgata gttcagatgg ttattttgcg accagttgtt cgccgcagtg aacacagcga
    17221 tagacggctt cgccacgaac tacgcgatta tggcggcgta cggtaagctg atgctcctgg
    17281 cacttgcagc ggtaggggaa ggtgttgcga cgcacggatt gcagttcgaa ctgatgcgta
    17341 cgacgggcgg gaacacccag cacgttttcc atcatccact tccactcttt gccatgtggt
    17401 gctacgcgcc cgaaatgttt ccataccagc aaatgtgcca gttcgtgcgg caccacttct
    17461 tcaataaagg cttcactgtt ttccaacagc aaaacgggat tgaggcggat ttcatagctt
    17521 tccagccagg ctgttccggc ggaggtgccg cgctgggtat aagagagttt tggctccggg
    17581 tagttacgcc ccaatttcag gttagcctgg gcgagttttt cccgcaggcg acgcataacg
    17641 gcctgttgga tggcgatagg gagacgggat gttttcatag cggcagagga tagagcgaag
    17701 aaaagatgac tgcaagaggt ggcttcctcc gcgatgggag gaagcttggg gagattaatc
    17761 gtgagcgcct atttcgcgca gtttacgacc tttcatcaga ttacgttcaa tatgttccag
    17821 cgaaacgtgt ttggtttccg gtaccagcca caatgtcagc aggataaaca gtacgttcag
    17881 agccgcatac acccagaagg tgttggcgtt acccagtgtg ttgagcatgg tcaggaacgt
    17941 tgcgccaacg atcatgttgg caatccagtt ggtggcggta gagcaggtga tgccaaaatc
    18001 gcggcctttc agcggctgaa tttcggagca cagtacccaa atcagcggac cggcactcat
    18061 ggcaaaaccg acaataaaca tcagcagcat ggcgatggcg aaatactgcg ctgacggaga
    18121 gtgaataccg atatgcatca ttgtaccgag tacgcccatg ccagcagcca tcaccaggaa
    18181 gcccagcgtt agcgttggtt tacgtcccca gcggtcaaca aggccgattg cgataaaggt
    18241 ggcaagtacg ttggtcaggc cgacaatcac tgtcccccac atttgctcgg tagtgttggt
    18301 ataacccgcc agttcgaaga ttttcggcgc gtaatacatg atgacgttca tcccggtgaa
    18361 ttgctgcatt acctgtaaca gtacgccaag gaacaccgcg cggcggaagt tgctattctc
    18421 tttaaacagc gcccagccac tctgtttaac ctgcaaactt tcacggattt catccagttc
    18481 gcgtttcgct tccgcgctgg tgtcacgcag gcgtagcagc acgcgttcgg catcaacaaa
    18541 acggcgtttg gcggcaaacc aacgtgggct gtctggcagg aagaagacac caatcagcag
    18601 caaaattgcc gggatgataa tcacacccag catccagcgc catgcaccgg tgtagctgaa
    18661 ggcggtatca gaaagataag caccgaggat cccgatagtg atcatcaact gatacatcga
    18721 gatcatactg ccacgaattt tttccggcgc aatttcagag aggtacagcg gtgcggtata
    18781 agaggccaca cccaccgcca gccccagtag aacgcgggaa agaatcagta cttcaacgtt
    18841 tggcgcagcc gcagagaaca gcgaaccggc aacaaacaaa attgcgccga tcatcaggct
    18901 ctttttgcgc ccgagtttaa aggagagcca gccgctgccc accgcaccga ctgccgcacc
    18961 gaacatcatg gagcttacga cccattcttg cgtgtgcgaa gtaatctgga attcatctgc
    19021 aataaacggc agtgcgccag caattacacc gatatccagg ccaaagagta atcccgccag
    19081 agcggcaagg aagcagacga aaaacgtcat tgccttgttt gaccgcccct gttttttagc
    19141 gtcaggcatg atgccctcca atatggttat tttttattgt gaattaagat aggtgagtac
    19201 gacgtaaaaa gatgtgaagc aaatcacatc agtgtaatcg cttacactaa catttatctt
    19261 agttatggtt taagtgcatg aaaataattg agttatatca tcatagatta aacgctgtta
    19321 tctgcaatta agactttact gaaaagaaat gtaacaactg tgaaaactga ataataatat
    19381 gaaaatatta caaatatttg gctggtgtta ttggtgtaat cggtttcagt agtgatgata
    19441 atttgcgata aaacgtgttg aggaagaatg atgttattgt gcgggcagct ttttgccgga
    19501 tgcggcgtga atgcctatcc accgcggtga ggtaaaagaa aaaggccagc gcgaggctgg
    19561 cctttgaaag caggtgaaga aagattactt cagaccggca gcatcgcgca gcagctgcgc
    19621 tttgtcggtt ttttcccacg ggaaatgttc acgaccaaag tgaccgtatg ctgcggtttc
    19681 tttgtagatc gggtgcagca gatccagcat ctgaatcaga ccgtatgggc gcaggtcgaa
    19741 gaactcacgt accagcagag tcagttgttc agaaggcact ttctcagtac cgaaagtttc
    19801 taccatgatg gaggtcggtt cagccacgcc gattgcgtag gaaacctgaa tttcacaacg
    19861 atcggccagg ccagcagcaa cgatgttttt cgcgacataa cgtgctgcgt aggctgcgga
    19921 acggtccact tttgatggat ctttaccaga gaatgctccg ccaccgtgac gtgccatgcc
    19981 gccgtaggta tcaacgataa ttttacgacc agtcagaccg cagtcaccca tcgggccacc
    20041 gataacaaaa cgaccggtcg ggttgatgaa gaatgtggtg gcagaagtca gccattcagc
    20101 gggcagaatt ggcttgatga tctcttccat taccgcttct tgcagcgatt tctggtcgat
    20161 ctcttcagag tgctgagtgg aaagcacgac agcatcaata ccaacgattt tgccgtcgtc
    20221 atactggaaa gtcacctggc ttttcgcgtc cgggcgcagc cacggcagag tgccgttttt
    20281 acgcacttca gcctgacgct gtaccagacg gtgtgcatag gtgataggtg ctggcatcag
    20341 cacgtcggtt tcattagttg cgtagccaaa catcagaccc tggtcacccg cgccctgttc
    20401 cagcggatcg gcacggtcaa cgccctggtt gatgtcagga gactgtttgc cgatagcgct
    20461 cagaaccgca caggagttag cgtcaaagcc catgtcggaa tgcacatagc caatttcgcg
    20521 aacggtgtta cgagtgatct cttcgatgtc tacccaggcg ctggtggtga tttcgccgcc
    20581 aactaaaacc atgccggttt ttacgtaggt ttcgcaagca acgcgtgctt tcggatcctg
    20641 ttcgaggatc gcgtctaaaa cggcatcaga gatttggtca gcaattttgt caggatgccc
    20701 ttcagagacg gactcggacg taaaaaggtg ttttgccata tttaatatca cctaaagaga
    20761 atttggttag ctcaaactgt tgtgtggatt ttctgtggta gcggatccta ccacgactct
    20821 gcaggttaaa aacactggca gtctgagtgt taatcggtat ggatggatta acatctagat
    20881 ggctatttta ggtcaattct tcacccgatt tccacttttt tttgaatcgt gtctcattct
    20941 gttaaaaacg tggctggaaa ttttttctga caatgccggc attctgcgta tttatatttt
    21001 gcaattttct gccattgtgg ggtataaaac gcggcgcgcg gcttaaataa aaagcacacg
    21061 acgtttcttt cgtgttgcca cttccagccg ggttgaaatc agagttttgg cttgtgggtt
    21121 cgtcttaaca ggcggccgtg gaggtgatac gaaataatga accgtagtct gctgcttaac
    21181 ctgtctcacc gttctggtga agattcgttc cccgcactct gcatctctgc tttgcatacc
    21241 tgccgatgtt atacccatct cggcgcttct caggattcaa gagctggtta cagttactga
    21301 ggactgaaca agggcgctct tgtaaaaaca agagttttct cgtggtttcg ccgaactgtc
    21361 acacttacgt tcggttatgt gcttaataat gttatgaaaa agaaaccggt tgcgcagttg
    21421 gagcgtcagc attcactgct ggaaaatcca tgtgcttatg ggttgttatc gcagttccag
    21481 gctgcgatag tcgttaactg ttttacactt aataaaataa tttgaggttc gctatgtctg
    21541 acgacatgtc tatgggtttg ccttcgtcag cgggcgaaca cggtgtacta cgctccatgc
    21601 aggaggttgc aatgagctcc caggaagcca gcaagatgct gcgtacttac aatattgcct
    21661 ggtggggcaa taactactat gacgttaacg agctgggcca catcagcgtg tgcccggacc
    21721 cggacgtccc ggaagctcgc gtcgatctcg cgcagttagt gaaaactcgt gaagcacagg
    21781 gtcagcgtct gcctgcactg ttctgtttcc cacagatcct gcagcaccgt ttgcgttcca
    21841 ttaacgccgc gttcaaacgt gcgagggaat cctacggcta taacggcgat tacttccttg
    21901 tttatccgat caaagttaac cagcaccgcc gcgtgattga gtccctgatt cattcgggcg
    21961 aaccgctggg tctggaagcc ggttccaaag ccgagttgat ggcagtgctg gcacatgctg
    22021 gcatgacccg tagcgtcatc gtctgcaacg gttataaaga ccgcgaatat atccgcctgg
    22081 cattaattgg cgagaagatg gggcacaagg tctatctggt cattgagaag atgtcagaaa
    22141 tcgccattgt gctggatgaa gcagaacgtc tgaatgtcgt tcctcgtctg ggcgtgcgtg
    22201 cacgtctggc ttcgcagggc tcgggtaaat ggcagtcttc cggcggggaa aaatcgaagt
    22261 tcggcctggc tgcgactcag gtactgcaac tggttgaaac gctgcgtgaa gctgggcgtc
    22321 tcgacagcct gcaactactg cacttccacc tcggttcgca gatggcgaat attcgcgata
    22381 tcgccacggg cgttcgtgaa tccgcgcgtt tctatgtgga actgcacaag ctgggcgtca
    22441 atattcagtg cttcgacgtc ggcggcggtc tgggcgtgga ctatgaaggc acccgttcgc
    22501 agtctgactg ctccgtaaac tacggcctca atgaatatgc caacaacatc atctgggcga
    22561 ttggtgatgc gtgtgaagaa aacggtctgc cgcatccgac ggtaatcacc gaatcgggtc
    22621 gtgcggtgac tgcgcatcac accgtgctgg tgtctaatat catcggcgtg gaacgtaacg
    22681 aatacacggt gccgaccgcg cctgcagaag atgcgccgcg cgcgctgcaa agcatgtggg
    22741 aaacctggca ggagatgcac gaaccgggaa ctcgccgttc tctgcgtgaa tggttacacg
    22801 acagtcagat ggatctgcac gacattcata tcggctactc ttccggcacc tttagcctgc
    22861 aagaacgtgc atgggcagaa caactctatc tgagcatgtg ccatgaagtg cagaagcaac
    22921 tggatccgca aaaccgtgcg catcgtccga taatcgacga actgcaggaa cgtatggcgg
    22981 acaaaatgta cgtcaatttc tcgctgttcc agtcaatgcc ggacgcatgg gggatcgacc
    23041 agttgttccc ggttctgccg ctggaagggc tggatcaagt gccggaacgt cgcgctgtgc
    23101 tgctggatat tacctgtgac tctgacggtg ctatcgacca ctatattgat ggtgatggta
    23161 ttgccacgac aatgccaatg ccggagtacg atccagagaa tccgccgatg ctcggtttct
    23221 ttatggtcgg cgcatatcag gagatcctcg gcaacatgca caacctgttc ggtgataccg
    23281 aagcggttga cgtgttcgtc ttccctgacg gtagtgtaga agtggaactg tctgacgaag
    23341 gcgataccgt tgcggatatg ctgcaatatg tgcagctcga tccgaaaacg ctgttaactc
    23401 agttccgcga tcaagtgaag aaaaccgatc ttgatgctga actgcaacaa cagttccttg
    23461 aagagttcga ggcaggtttg tacggttata cttatctgga agatgagtaa gtccgtatta
    23521 cttgaatccg cttaatttag cggtgataat ccgccacaat ttattgtgac aaatccaacc
    23581 cttcctcgtc gggcctaacg acgcggaagg gtttttttat atcgactttg taataggagt
    23641 ccatccatga gcaccttagg tcatcaatac gataactcac tggtttccaa cgcctttggt
    23701 tttttacgcc tgccgatgaa cttccagccg tatgacagcg atgctgactg ggtgattact
    23761 ggtgtgccgt tcgatatggc cacttctggt cgtgcggggg gacgccacgg tccggcagcg
    23821 atccgtcagg tttcgacgaa tcttgcctgg gaacacaatc gcttcccgtg gaatttcgac
    23881 atgcgtgagc gcctgaacgt cgtggactgc ggcgatctgg tatatgcctt tggcgatgcc
    23941 cgtgagatga gcgaaaaact gcaggcacac gccgagaagc tgctggctgc cggtaagcgt
    24001 atgctctctt tcggtggtga tcactttgtt acgctgccgc tgctgcgtgc tcatgcgaag
    24061 catttcggca aaatggcgct ggtacacttt gacgcccaca ccgataccta cgcgaacggt
    24121 tgtgaatttg accacggcac catgttctat accgcgccga aagaaggtct gatcgacccg
    24181 aatcattccg tgcagattgg tattcgtacc gagtttgata aagacaacgg ttttaccgtg
    24241 ctggacgcct gccaggtgaa cgatcgcagc gtggatgacg ttatcgccca agtgaaacag
    24301 attgtgggtg atatgccggt ttacctgacc tttgatatcg actgcctaga tcctgctttc
    24361 gcgccgggaa ccggtacgcc agtgattggc ggcctgacct ccgatcgcgc cattaaactg
    24421 gtacgcggcc tgaaagatct caacattgtt gggatggacg tagtggaagt ggctccggca
    24481 tacgatcagt cggaaatcac cgctctggct gcggcgacgc tggcgctgga gatgctgtat
    24541 attcaggcgg cgaaaaaggg cgagtaagca ccagatgcga tgcgcacggg taaaacgtgc
    24601 cattaatgtc ggatgcggcg tgaacgcctt atccgaccta cgttcggcac ccgtaggccg
    24661 gataagatgc gccagcatcg catccggcaa tgcgcacaag gtaacaaatg tgccattaat
    24721 gtcagatgcg gcgtgaacgc cttatctgac ctacgttcga caccaccagg ctttacttaa
    24781 tcccatccgc gctcatgcga tcgcgaatat gctgggcgcg ttcggcggat gccggatggt
    24841 cgtcaaacat tgagctttgg cggccttctt ccagtttcgc cagtttttca aagctggtgg
    24901 caagacctgc cgggctgatg ccgcgttggc gcagaagatc gtaagaataa tcatcggctt
    24961 ctgcttcctg gcgctgggag aattgcgaat tgactaattt ctcgcccaga tcaccaagtt
    25021 gtgattgaga taaactgccg acaatcccgc ccgcagaggc cgcagctact cgcacggcat
    25081 ttgtaccaag cgccacctgc atccctttct tcacatggcc taacgccacg tgccccatct
    25141 cgtgaccgat caccgcttcg acttcgttat ccgtcatcat atccatcagc ccgctataga
    25201 cgcggataca gccgttagcc attgcaaagg cgttcacatc cttcgccata tacactttgt
    25261 aattcaccgg ctgaccgttg atattgttgc ctagcgcatt ggcaatagtt gtcagacgtt
    25321 tagcgtattc gctattggct ggcgcaatcg tcgccttgct gtccatctcc tgacatgcct
    25381 gatcgctcag ggttttcacc tgcgcatcac tcaaactgta agcctgaaaa gcttccgctc
    25441 ctgatgagag cagtccgttg gagtccatat tctggcaacc agtcagtacc gttgccacgc
    25501 tcattgctac caataaggcg cgaattttca tgttttctct tccgcacaag gtcaggatta
    25561 ttctaaaaat gcgccgtttg caggtgaatc gacgctcagt ctcagtataa ggaaaagcgc
    25621 agcggacggg cgagtagatt gcgcaacatg cgagcatgat ccagagattt ctgaagcggc
    25681 aaaaggatgt tccatgtaca tgacgcgcgg cttgcggtaa attgttggca aattttccgg
    25741 cgtagcccaa aacgcgctgt cgttaagggc gtgcccttca tcatccgatc tggagtcaaa
    25801 atgtcctcac gtaaagagct tgccaatgct attcgtgcgc tgagcatgga cgcagtacag
    25861 aaagccaaat ccggtcaccc gggtgcccct atgggtatgg ctgacattgc cgaagtcctg
    25921 tggcgtgatt tcctgaaaca caacccgcag aatccgtcct gggctgaccg tgaccgcttc
    25981 gtgctgtcca acggccacgg ctccatgctg atctacagcc tgctgcacct caccggttac
    26041 gatctgccga tggaagaact gaaaaacttc cgtcagctgc actctaaaac tccgggccac
    26101 ccggaagtgg gttacaccgc tggtgtggaa accaccaccg gtccgctggg tcagggtatt
    26161 gccaacgcag tcggtatggc gattgcagaa aaaacgctgg cggcgcagtt taaccgtccg
    26221 ggccacgaca ttgtcgacca ctacacctac gccttcatgg gcgacggctg catgatggaa
    26281 ggcatctccc acgaagtttg ctctctggcg ggtacgctga agctgggtaa actgattgcg
    26341 ttctacgatg acaacggtat ctctattgat ggtcacgttg aaggctggtt caccgacgac
    26401 accgcaatgc gtttcgaagc ttacggctgg cacgttattc gcgacatcga cggtcatgac
    26461 gcggcatcca tcaaacgcgc agtagaagaa gcgcgcgcag tgactgacaa accgtccctg
    26521 ctgatgtgca aaaccatcat cggtttcggt tccccgaaca aagccggtac tcacgactcc
    26581 cacggtgcgc ctctgggcga cgctgaaatt gccctgaccc gcgaacagct gggctggaaa
    26641 tatgcaccgt tcgaaatccc gtctgaaatc tatgctcagt gggatgcgaa agaagcaggc
    26701 caggcgaaag aatccgcatg gaacgagaaa ttcgctgctt acgcgaaagc ttatccgcag
    26761 gaagccgctg aatttacccg ccgtatgaaa ggcgaaatgc cgtctgactt cgacgcgaaa
    26821 gcaaaagagt tcattgctaa actgcaggct aacccggcga aaatcgccag ccgtaaagcg
    26881 tctcagaatg ctatcgaagc tttcggtccg ctgttgccgg aattcctcgg cggttctgct
    26941 gacctggcac cgtctaacct gaccctgtgg tctggttcta aagcaatcaa cgaagatgct
    27001 gcgggtaact acatccacta cggtgttcgc gagttcggta tgaccgcgat tgctaacggt
    27061 atctccctgc acggtggttt cctgccgtac acctccacct tcctgatgtt cgtggaatac
    27121 gcacgtaacg ccgtacgtat ggctgcgctg atgaaacagc gtcaggtgat ggtttacacc
    27181 cacgactcca tcggtctggg cgaagatggc ccgactcacc agccggtaga gcaggtcgct
    27241 tctctgcgcg taaccccgaa catgtctaca tggcgtccgt gtgaccaggt tgaatccgcg
    27301 gtcgcgtgga aatacggtgt tgagcgtcag gacggcccga ccgcactgat cctctcccgt
    27361 cagaacctgg cgcagcagga acgaactgaa gagcaactgg caaacatcgc gcgcggtggt
    27421 tatgtgctga aagactgcgc cggtcagccg gaactgattt tcatcgctac cggttcagaa
    27481 gttgaactgg ctgttgctgc ctacgaaaaa ctgactgccg aaggcgtgaa agcgcgcgtg
    27541 gtgtccatgc cgtctaccga cgcatttgac aagcaggatg ctgcttaccg tgaatccgta
    27601 ctgccgaaag cggttactgc acgcgttgcg gtagaagcgg gtattgctga ctactggtac
    27661 aagtatgttg gcctgaatgg cgctatcatc ggtatgacca ccttcggtga gtcagctccg
    27721 gcagagttgc tgttcgaaga gttcggcttc accgtcgaca acgtcgttgc gaaagcaaaa
    27781 gaactgctgt aattagcatt tcgggtaaaa aggtcgcttc ggcgaccttt tttattacct
    27841 tgatatgtcc gtttgcggac aagcaataga taaagcgtgt tgcagatcac aaatatttat
    27901 atgcaataaa tatcaattat gtaatatgca tcacgatatg cgtattgaca tttgttgtta
    27961 tatctataac tcaatgttat ataagaaatt aataattcac tgttttcaaa acaccggttt
    28021 ccctgctcaa ttgctttcat taaaccgcac gagtatttta tttttaccgc cggacggtac
    28081 cctaaaaata aaataaggat aatttatgcg gcttagtgac tattttccag aatcatcaat
    28141 ctcagttata cattcagcaa aagattggca ggaagctatc gatttctcga tggtatcatt
    28201 gctggataaa aactatatca gcgagaatta cattcaggca attaaagatt ccaccattaa
    28261 caatggccct tattatattc tcgcaccagg cgtggcaatg cctcatgcgc gaccggaatg
    28321 tggggcgctt aaaaccggga tgtctttgac attacttgaa caaggtgttt attttccggg
    28381 gaatgacgaa ccaattaaat tactcatcgg actctctgct gccgatgccg attcgcacat
    28441 tggcgctatt caggcgttaa gtgaattact gtgcgaagaa gaaatactcg aacaactctt
    28501 aacagcatca tcagaaaaac aattagcgga cattatcagc cacagataat tactctctcc
    28561 tctacaggat aatattatgg aaaacaagtc tgctcgtgca aaggtccagg cttttggggg
    28621 ctttttgact gcaatggtca tccccaatat tggtgctttt attgcctggg gttttattac
    28681 tgcgttattt attcccaccg gttggctgcc taacgaacat ttcgccaaaa ttgtcggccc
    28741 gatgattacc tatttattgc ccgtgatgat tggttctaca ggtggtcatc tggtcggcgg
    28801 taaacgcggg gcggtcatgg gcggaatagg tactattggt gtgatcgttg gcgcagagat
    28861 cccgatgttc cttggctcaa tgatcatggg gccgctcggt gggttggtca taaaatatgt
    28921 cgataaggca ctggaaaaac gcatacctgc cggttttgag atggttatca ataacttctc
    28981 attaggtatc gcggggatgc tcctttgtct gctgggtttt gaagtgatcg gcccggcggt
    29041 gttaattgcc aatactttcg tcaaagagtg tatcgaggcg ctggtacatg cgggttatct
    29101 gcctctgctg tcagtcatca atgaaccggc gaaagtgctt ttccttaata atgcgatcga
    29161 tcagggcgtc tattatccgc tgggaatgca acaggcttcg gttaacggta aatccatctt
    29221 ctttatggtg gcctctaacc caggtccggg cctggggctg ctgctggcgt ttaccttgtt
    29281 tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg attattcact tcctcggtgg
    29341 gatccacgaa ctgtatttcc catatgtgct gatgaagccg ctgactatta ttgccatgat
    29401 tgcgggcggt atgtctggta cctggatgtt taacttactg gacggtggtc tggtggctgg
    29461 cccaagtctg gggtctatct ttgcttacct ggcactgacg ccgaaaggct cgttcctggc
    29521 gacaattgcc ggtgttacgg taggtaccct ggtgtccttt gctattactt cgctgatact
    29581 gaagatggaa aaaacagtgg aaacggagag cgaagatgag tttgctcagt caaccaatgc
    29641 ggttaaggcg atgaaacaag agggtgcatt ctcgttaagc agggttaagc gtatcgcctt
    29701 tgtttgcgat acggggatgg gctccagtgc gatgggcgcg accaccttcc gtaaacgcct
    29761 ggaaaaagcg gggctggcaa tagaagtaaa acattacgcg atagaaaacg tgcctgcgga
    29821 tgccgatatc gtcgtcactc acgccagtct ggaagggcgc gtgaaacgtg tgacggataa
    29881 accgctgata ttgattaata actatattgg cgatccaaaa ctcgacactt tatttaatca
    29941 attaaccgcc gaacataaac actgattaca gaggtaaaaa tgaaaaccaa agttgctgct
    30001 atttatggca agcgggatgt ccgtctgcgc gaatttgaac tgccagaaat taccgataat
    30061 gaactgttag tgagtgtaat ttctgacagc gtctgtttat cgacctggaa agcggcgtta
    30121 ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt cattaccggg
    30181 catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggcaa atataaaaaa
    30241 ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc agcaggctac
    30301 agctacgaat attttggcgg taacgccacc tatatgatta ttcccgaaat cgccattaat
    30361 ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct ggtagagcct
    30421 atgtgctgca ttattggtgc ttatcatgcc aattatcaca ccacgcaata tgtttatgag
    30481 catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc gggtccgatg
    30541 ggcattggcg ctatcgatta cgccattaac ggtggcatac aaccgtcacg ggtggtggtg
    30601 gtcgatatcg acgacaaacg cctggcgcag gtgcagaagc tgctgccggt ggatctggcg
    30661 gccagtaaag gcattgagct ggtgtatgtg aataccaaag ggatgagcga tccggtccag
    30721 acgctgcggg cgctgacagg agatgtcggg ttcgatgaca tttttgttta tgcggcggtg
    30781 cctgctgtcg ttgagatggc tgacgaatta ctggcggaag atggctgtct gaacttcttt
    30841 gccgggccga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt ccattacaac
    30901 agcacgcacg tagtcggtac atccggcggt tcaacggacg acatgaaaga ggcgattgcg
    30961 ttaagcgcca cggggcagtt gcagccgtcc tttatggtca cgcatatcgg tgggctggat
    31021 gcggtgccag ataccgtgct caatctgccg gatatccctg gcggtaaaaa actcatttat
    31081 aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg caaaaccgat
    31141 ccgctattta aagagttggc gcggctggtt gaggaaacgc acggcatctg gaatgaacag
    31201 gccgagaaat atctgctggc acaatttggc attgatatcg gggaggccgc gcaatgatgt
    31261 ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc tggccgcaaa
    31321 ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg cgccaggcat
    31381 taaacgacgt cgcttttcgt ggacgagtgg ttatcggcga aggagaaatt gaccatgcgc
    31441 cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc gatatcgcgg
    31501 ttgatcccat tgaaggcacg cggatggtgg cgatggggca gagcaatgcg ctggcggtca
    31561 tggctttcgc ccctcgggat agcctgttac atgcgcccga tatgtatatg aaaaagctgg
    31621 ttgttaatcg actggctgcc ggggtgattg atctgtcttt gcctctggcg gacaacttgc
    31681 gtaacgtggc gcgggcgtta ggtaaaccgc tggacaagct gcgcatggtt acgctggata
    31741 aaccgcgcct gagtgccgcg attgaggaag caacacagct gggcgtgaag gtttttgccc
    31801 tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat ccttacgatg
    31861 tgatgtacac catcggcggt gcgccagaag gcgtgatttc tgcctgtgcc gttaaagcat
    31921 taggcgggga tatgcaggcc gagctgattg acttttgtca ggctaaaggg gattacacgg
    31981 aaaatcggca gattgcagag caggagagca agcgttgcaa ggcaatgggc gttgacgtta
    32041 accgtgtgta ctcgctcgat gaactggtga gggggaacga tatcctcttt agcgctacgg
    32101 gcgtgacggg cggcgagttg gtgaacggta tccaacagac ggcgaatggg gtgcggacgc
    32161 agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc ctgcattgat
    32221 ggtgatctat cggaagacca atggcgaagc tgacagaaga tgatgtgctt gagcaactgg
    32281 atgcacagga caatttattt tcatttatga aaactgcgca ttctattttg ctccagggga
    32341 tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagatcgtg gaatatgcag
    32401 tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgcg ctgcgtttga
    32461 tttatgcgct ggggaaaatg gataaatggc tgtacgccga tatcacgcat ttttcccagt
    32521 tctggcatta cctgaacgaa caggatgaaa cgcccggatt tgccgatgac atgacctggg
    32581 attttatcag caacgtcaat agcataaccc gcaatgcgat gctctatgac gcgttgaaag
    32641 cgatgaagtt cgccgatttc gccgtctggt cagaggcgcg ttttagcggg atggtcaaaa
    32701 cggcgctgac gctggcagta acgacaatct taaaggaatt aacgccgtga aaattgaatt
    32761 aacggtgaat gggctgaaga tacaggcaca gtaccagaac gaagagattg aaaatgtaca
    32821 taaaccgttg ctgcgtatgc tggcggcctt acagacggta aatccgcagc ggcgtacggt
    32881 ggtttttctt tgcgctccgc cggggacggg taaatctacc ctaaccacct tctgggaata
    32941 tctcgcgcaa caagacccgg agctgcccgc tattcagacg ctcccgatgg atggttttca
    33001 ccattacaat agctggctgg acgcgcatca attgcgcccc ttcaaaggcg caccagagac
    33061 atttgacgtt gcgaaactgg cggaaaatct gcgccaggtg gtggaagggg attgtacgtg
    33121 gccgcagtac gatcgacaaa agcatgatcc tgttgaagat gcgttgcacg ttaccgcacc
    33181 actcgtcatc gtcgaaggaa actggttgtt gctggacgac gagaagtggt gccagcttgc
    33241 gcaattttgt gatttctcga tctttatcaa cgcgccagct actgcgttgc gggagcgtct
    33301 ggtggggcgc aagctggcgg gaggggtatc gctggcagat gcagaagcct tttatgaccg
    33361 taccgacggg ccgaatgttc gccgggtgct ggaagagagt ctgcctgcaa atttgacctt
    33421 aatgatgacc gctacgggtg aatatcgtct ggtggattaa cgttcctgat tacaccatct
    33481 tccagtcgca ccacacgaga gaaatgacgc cgggtaaatg cggcgtcatg actgattgct
    33541 accacgctcg tgcctcgctg gcggcacttc tccagccagc tttcaaacac gctcaaccaa
    33601 ttttcgtcaa aatcccgact cggctcgtcc agcagtaata gtggcggaga gagtgcttcc
    33661 aggcaggcga cggcgaccat ccgtcgctgc gcactatgca gatccaacgg gtgcgagttg
    33721 gcagtgtctg ctaactgaca acattgcagg gcagcatggg tacgttgagt gatttcatcc
    33781 gccgggcatt tttgcagttt caaaccaaat gcgatctcgt cagcgacggt gctgtgaaaa
    33841 agttggtttt ccgcttcctg aaacagtacg ccaacctttg ccgcgcgctg gcggtttttc
    33901 aggtttttca tcgcctgttg ttgcagcata accgtaccgg ctgtgggcgt aagaagacct
    33961 gccattactc gtaacagcgt cgatttccct gcgccattgt caccggtcag cgccaaccat
    34021 tcaccttgtt tgagttgcag agaaatgtcg caaagacaat ccgttgccgc gccaggccag
    34081 cgataagata tctgatttaa cgttaacata gtggctggag cgctccgttt tgcaattgcc
    34141 acgcttgctg gcagtagttc aggaaaggtg tgcgatgacg ttcaaaaaga ataatcagcg
    34201 aaccacgttc aaacgcccag tgttgtaagc gctgcaacag catttcgctg gcctgcggcg
    34261 tcaggcggct aaaggcttca tcaagaatta agagctttgg ctgcatcgcg atggcgcagg
    34321 cgatgaccac gcgctgtgtc tctccaccgg agagtgttgc cgggtgacga tggcgcaacg
    34381 gctggcactc ggtcagagtc agggcggcat caatccgcgc cataatctct gcttcagcaa
    34441 gacacagatt ttctggccca aaggcaactt cttcttccac gctaaaggta cagccggaga
    34501 gctgcaaata gggcgattgc tggacgagct ggatggtggc ggattgttca ttaagcggga
    34561 gatgcccaat gggcgtaccg aggagcgtcc ccgtgcctgt tatctctcct ggcagaaagt
    34621 ccggatacca gcccgccatc agctgtgcca gcgtactttt gccactgccg ttgtcaccaa
    34681 agatggcgac catccccggt ttgacatagt gaaagtcata acagaacgga ggatgcgttg
    34741 agtgtgtcgg gcaataacgg aactgttcta acgtaaccat atccacgctc cgaattctgt
    34801 cagcatcagc aagatcatgg tgtaacgcgc tacgcgttgt agggtgctat cggctggtgc
    34861 ccacaacgtt gttcgtcggt tgtggatccg aaatgcccgc atatccagcg ccgcaccacg
    34921 gacagccaga tcgttaagcg cattgtgtgt tagcggaata attaacgcgg gcatcgcacg
    34981 taaacgctga taccagccct catccagcgg cacgccgcgg gcacgctgcg cttcatgaat
    35041 aatcgctaat tgccgtttta actgctcaac gaccaataac ggcccggcaa acagataagc
    35101 gacgcctggc ggcaggcgag aagcaaacag agcgcgaata aaacattgca cggggacgta
    35161 ctgcatccac aactgtgagg ttgaaacaat caccagaatc cgcaaccaga gcgtaatagc
    35221 atgcgaccac cgttctggcg agcgtggcgt gccgcttagc cattctggta gccagccacc
    35281 gtgtaccagc cacagcccgg ctcccagcga gaacatcagc cagacgacat attttgcccg
    35341 ccgccgcgtg gcctttagcg caataaggca gaaaaaggtc gccgcgctat agatcggcaa
    35401 aatggtctgc gcgggcagga taagcgtggt gcaggccgcc aatgcccaca gtgttaatga
    35461 ggtaaatgga tgcatttagc gcacggcagc cattgccgga aaatggcgcg tggtgcgaat
    35521 cggcagctgg cgcagtaaaa gccagacaat caccgctgta agaattttat cgaccaggtt
    35581 cgcgcctatc accgtaatcg ccaccgactc caccaggttt tgccccatcg agtgcatcca
    35641 ggcaacgaac aaatcggctc cgctaccggt gacaccgccg aacagcgcag tacgcagcgg
    35701 tacagcgacg accgttaccg caagcgtaat aatcacgccg ctgacaacga cttttggcag
    35761 ggtgcgaaac caacccgctc gcgccagcca accggcgacc aggccgatga ccatcgcgac
    35821 gggtgcaaat gcggcggcga tcgggtcggt cagcaatccc cagagtaaat tggtcagtaa
    35881 gccggtcagc attccgatca ccggaccaag cagtaccgcg ctaataagtg tgccgatgga
    35941 gtcgagaaat ataggtaatt tgaccatgct ggccagttgc ccgccgatca tattgatggc
    36001 gatagatata acgatcaaca ccagtgcctg actggaaaag tggctacgcg ccataagaaa
    36061 acctctttga aagtagttat ttaacggggg aaatatgcaa ctcgtcgtaa ccagaaagcg
    36121 cgcaaggttg gcggctaaac gccagttcct caaggctacc gatcaccagt tcaagcgtgg
    36181 tccgcacggt acagccgggc atcatatgac cgccaagcat cgtgccgtgt ggatcggaaa
    36241 cgcagagatg aagatgctca ccactctgtt ccagcgtgcc gtttagtgag atcacttcga
    36301 atgtaccgtt caggagcgtg gtgccttctt gcctggcata gcgcaaggcg acgtcagtca
    36361 ggctaccggt acagcctgca atccaggcgg catgaagttg atgctgctgt gcaaaagcgt
    36421 gtagctggga aaaaacttcc tgcccgggga ggaggcgcag tgcgtaaaag cgtgcggagg
    36481 aagaatatgg gtagggcaat gtcatcattg gagtactccg aaactcggac gtattgcgga
    36541 taatatcgac gctgcatcag tcatattctg atataagtca gggtttgtga cggcacaacg
    36601 aaaagatttt gctacgctta ttcgtgattc tgcaaagtgg aataaattct acaggggcgg
    36661 ggctgaaaac gtgacgtaag tcagataatt gctttctttg actggacaat cattcctttt
    36721 attccacgtt tcgcttatcc tagctgaagc gtttcagtcg attaaatgtt cgacaattaa
    36781 ccaatcagtc gcagtttgcg acaggtaagg tttccccgga cgatttgctg gattactctg
    36841 tctggcaaat ttgctcaggg aaaaccttgc aggagatcta tgaccgtacg cgtagcgata
    36901 aatggcttcg gtcgcatcgg gcgtaatgtg gttcgtgctt tgtatgaatc cggacgccgg
    36961 gcggaaatta ccgtggtggc aatcaacgaa ctggcggatg ctgcgggcat ggcgcatttg
    37021 ttgaaatatg acaccagcca tggccgtttt gcatgggaag tacgacagga acgcgatcaa
    37081 ctttttgttg gtgatgacgc catccgcgta ttgcatgaac gttcactgca atcgctcccc
    37141 tggcgtgaac ttggcgttga tgtagtcctc gactgcaccg gcgtatatgg ctcccgcgag
    37201 catggcgaag cgcatattgc cgccggggcc aaaaaagtgc tcttttcaca tcctggcagt
    37261 aacgatctcg acgcgaccgt tgtttacggc gtcaatcagg atcaacttcg tgcggaacac
    37321 cgcatcgttt ctaacgcttc ctgtaccacg aattgcataa ttcccgtcat caaattgtta
    37381 gatgatgcgt acggtattga gtccggcact gtgaccacaa ttcactccgc catgcacgat
    37441 caacaggtta ttgatgcata ccatcctgac ctgcgtcgca cccgggcagc cagccagtcg
    37501 atcattccgg tcgatactaa actggccgcc ggtatcacac gattttttcc gcaatttaac
    37561 gatcgctttg aagcgattgc ggtacgtgtg ccaaccataa atgtgacggc aatcgattta
    37621 agcgtgacgg tgaagaaacc tgtaaaagcc aatgaagtca acctgttgct gcaaaaagca
    37681 gcacaaggtg catttcatgg tatagttgac tatacggaat tgccgttggt ctctgtagat
    37741 tttaaccacg atccgcacag tgccattgtc gatggcaccc aaacccgggt cagtggcgca
    37801 cacctgatca aaacgttggt ctggtgcgat aacgaatggg gctttgctaa ccgaatgctc
    37861 gacacgacgt tagctatggc tactgttgct ttcaggtaag acgcaagcag cgtctgcaaa
    37921 acttttagaa tcaacgagag gattcaccat gtctgtaatt aagatgaccg atctggatct
    37981 tgctgggaaa cgtgtattta tccgtgcgga tctgaacgta ccagtaaaag acgggaaagt
    38041 aaccagcgac gcgcgtatcc gtgcttctct gccgaccatc gaactggccc tgaaacaagg
    38101 cgcaaaagtg atggtaactt cccacctggg tcgtcctacc gaaggcgagt acaacgaaga
    38161 attctctctg ctgccggttg ttaactacct gaaagacaaa ctgtctaacc cggttcgtct
    38221 ggttaaagat tacctcgacg gcgttgacgt tgctgaaggt gaactggttg ttctggaaaa
    38281 cgttcgcttc aacaaaggcg agaagaaaga cgacgaaacc ctgtccaaaa aatacgctgc
    38341 actgtgtgac gtgttcgtaa tggacgcatt cggtactgct caccgcgcgc aggcttctac
    38401 tcacggtatc ggtaaattcg ctgacgttgc gtgcgcaggc ccgctgctgg cagctgaact
    38461 ggacgcgctg ggtaaagcac tgaaagaacc tgctcgcccg atggtggcta tcgttggtgg
    38521 ttctaaagta tctaccaaac tgaccgtttt ggactccctg tctaaaatcg ctgaccagct
    38581 gattgttggt ggtggtatcg ctaacacctt tatcgcggca caaggccacg atgtgggtaa
    38641 atccctgtac gaagctgacc tggttgacga agctaaacgt ctgctgacca cctgcaacat
    38701 cccggttccg tctgatgttc gcgtagcaac cgagttctct gaaactgcac cggctaccct
    38761 gaaatctgtt aacgatgtga aagctgacga gcagatcctg gatatcggtg atgcttccgc
    38821 tcaggaactg gctgaaatcc tgaagaatgc gaaaaccatt ctgtggaacg gtccggttgg
    38881 cgtgttcgaa ttcccgaact tccgcaaagg tactgaaatc gtggctaacg ctatcgcaga
    38941 cagcgaagcg ttctccatcg ctggcggcgg cgacactctg gcagcaatcg acctgttcgg
    39001 cattgctgac aaaatctcct acatctccac tggcggcggc gcattcctcg aattcgtgga
    39061 aggtaaagta ctgcctgcag tagcgatgct cgaagagcgc gctaagaagt aaaaaatcac
    39121 agggcaggga aacctgccct tgtttcagcg cgcttttaga gcacgcattt tttcatactc
    39181 taaataattc gagttgcagg aaggcgacaa gagagtgaat ccccaggagc ttacataagt
    39241 aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat gatgagtata
    39301 aggcccgacg atacaggaca agagacatgt ctaagatttt tgatttcgta aaacctggcg
    39361 taatcactgg tgatgacgta cagaaagttt tccaggtagc aaaagaaaac aacttcgcac
    39421 tgccagcagt aaactgcgtc ggtactgact ccatcaacgc cgtactggaa actgctgcta
    39481 aagttaaagc gccggttatc gttcagttct ccaacggtgg tgcttccttt atcgctggta
    39541 aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct gggcgcgatc tctggtgcgc
    39601 atcacgttca ccagatggct gaacattatg gtgttccggt tatcctgcac actgaccact
    39661 gcgcgaagaa actgctgccg tggatcgacg gtctgctgga cgcgggtgaa aaacacttcg
    39721 cagctaccgg taagccgctg ttctcttctc acatgatcga cctgtctgaa gaatctctgc
    39781 aagagaacat cgaaatctgc tctaaatacc tggagcgtat gtccaaaatc ggcatgactc
    39841 tggaaatcga actgggttgc accggtggtg aagaagacgg cgtggacaac agccacatgg
    39901 acgcttctgc actgtacacc cagccggaag acgttgatta cgcatacacc gagctgagca
    39961 aaatcagccc gcgtttcacc atcgctgcgt ccttcggtaa cgtacacggt gtttacaagc
    40021 cgggtaacgt ggttctgact ccgaccatcc tgcgtgattc tcaggaatat gtttctaaga
    40081 aacacaacct gccgcacaac agcctgagct tcgtattcca cggtggttcc ggttctactg
    40141 ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa aatgaacatc gataccgata
    40201 cccaatgggc aacctgggaa ggcgttctga actactacaa agcgaacgaa gcttatctgc
    40261 agggtcagct gggtaacccg aaaggcgaag atcagccgaa caagaaatac tacgatccgc
    40321 gcgtatggct gcgtgccggt cagacttcga tgatcgctcg tctggaaaaa gcattccagg
    40381 aactgaacgc gatcgacgtt ctgtaagata ttcctttctg cttatctcaa ggcccgctct
    40441 gcgggtcttt ttttcgccaa aagcaaaatt gcctgatgcg ctacgcttat caggcctaca
    40501 cgtatcctgc aatatattga atttgcaggt ttttgtaggc cggataaggc gttcacgccg
    40561 catccggcat gacaaagcgc acattgatat ctgtctgagc cccgctctgc ggggattttt
    40621 tcgccaaaag caaggtgatt cagatgagaa atctgtgacc tatttggcaa aattatgctt
    40681 tattgtttac ccttgtcaga ctgcccgtca taaggcggcg gagtgtattt ctccattttg
    40741 agtcagttga aaaggaatat tgaatggaag atttgaatgt tgtcgatagc ataaacggcg
    40801 cgggaagctg gctggtagct aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg
    40861 cggcactcgc gatcatcatc gttggtttga ttatcgcgcg gatgatttcc aacgcggtga
    40921 atcgcctgat gatctcccgt aaaatcgatg ccactgttgc tgattttctt tctgcattag
    40981 tccgttacgg tattatcgcc tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa
    41041 cagcgtcagt cattgctgta ctcggtgccg caggcttagc tgttggtctg gctttgcagg
    41101 ggtcactttc taacctggcc gctggcgtgt tacttgtcat gttccgaccg ttccgtgccg
    41161 gagaatatgt tgacctcggc ggcgtagccg gtactgtgct gagtgtgcag attttctcca
    41221 ccaccatgcg tactgcagac ggtaaaatta tcgttattcc gaacggtaaa attattgccg
    41281 gaaatattat taacttctcc cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg
    41341 cgtatgattc cgatatcgat caggttaagc agatcctgac caatattatc cagtctgaag
    41401 atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga
    41461 ttaatttcgt ggtccgcgtc tggagcaaca gcggcgatct gcaaaacgtg tactgggatg
    41521 tgctggagcg tattaaacgt gaatttgatg ccgccggtat cagcttcccg tacccgcaaa
    41581 tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag
    41641 ataatactct ctggcctctc tcttattagt tattctgatt gccaattaat attatcaatt
    41701 tccgctaata acaatcccgc gatatagtct ctgcatcaga tacttaattc ggaatatcca
    41761 acgtgttttc ttattacttt caaggtcttg atcttggggc agctatgatt ctgccgctcg
    41821 gcccacaaaa tgcttttgtg atgaatcagg gcattcgtcg tcagtaccac attatgattg
    41881 ccttactttg cgctatcagc gatttggtcc tgatttgcgc ggggattttt ggtggtagcg
    41941 cgttattgat gcagtcgccg tggttgctgg cgctggtcac ctggggcggc gtagtcttct
    42001 tgctctggta tggttttggc gcttttaaaa cagcgatgag tagtaatatt gagttagcta
    42061 gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc caccatgttg gcagtgacct
    42121 ggctgaatcc gcatgtttac ctggatactt ttgttgtact gggcagcctt ggcgggcagc
    42181 ttgacgtcga accaaaacgt tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt
    42241 tctttggtct ggctattctc gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac
    42301 agcgcattat caatctggta gtgggatgtg tcatgtggtt tatagccttg cagctggcga
    42361 gagacggtat tgctcatgca caagccttgt tcagttaggc gctatctgat ggaaaaataa
    42421 aacagaggcg ctaagcttgc ctccagaggt cctgaatttt gggcaagtca attaacacgg
    42481 agagactaac gtgaagttca aagttatcgc cctagcggca ttaatgggta ttagcgggat
    42541 ggcagcgcag gctaacgaat tgccggatgg accgcatatt gtcacctccg gtacggcaag
    42601 tgtggatgcg gtgccagaca ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga
    42661 tgccgctaca gccaagaaac aggcagatga gcgcgtcgca caatacattt ccttccttga
    42721 actcaatcag atcgcgaaaa aagatatcag ctcagcgaat ttacgcaccc agccagatta
    42781 tgattatcag gatggtaaaa gtatcctgaa aggctaccgc gctgtgagaa cggtggaagt
    42841 cacgctccgt cagttagaca aactgaattc cttgctggat ggcgcgctga aggcgggtct
    42901 taacgaaatt cgttcagtgt cgctgggcgt ggcgcagccg gatgcctata aagacaaagc
    42961 gcgtaaggca gcgattgata acgcgattca tcaggcgcag gaactggcga acggctttca
    43021 tcgtaaactg gggccggtat atagcgtgcg ctaccatgtt tccaactatc agcccagccc
    43081 aatggtgcgg atgatgaaag ccgatgccgc gccggtgtct gcccaggaaa cttacgagca
    43141 ggccgctatt cagtttgatg atcaggtcga tgtggtcttc cagttagaac ctgtggatca
    43201 acaacccgct aaaacacctg cagcacaata atacttgtag cccgacgtat tcgtcgggct
    43261 aatgccttta cccgatattg cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat
    43321 cacctatctt tgctcgcggt ttcatcaagg ataggctgac tgaaacggtt aagcggacaa
    43381 ggatgcgggg aggtgcccct attaagttgt caagcatgtt atgacccctg cggggtataa
    43441 aaagtcccgt cgcgcatcat ggcgaacaga a
//
LOCUS       DAJVTY010000010        42149 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVTY010000010 DAJVTY010000000
VERSION     DAJVTY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42149
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.010"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..466
                     /locus_tag="OQQ66_000554"
     CDS             <1..466
                     /locus_tag="OQQ66_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1903595.1"
                     /translation="LSAVAEAEQQGKNEEQRQHDEWVAERNREIQQEKQRRANAQVAA
                     NKRAATAAANKKARQDKLDAEASADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRE
                     NEFIDQELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
     gene            complement(561..2138)
                     /gene="guaA"
                     /locus_tag="OQQ66_000555"
     CDS             complement(561..2138)
                     /gene="guaA"
                     /locus_tag="OQQ66_000555"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1903596.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2207..3673)
                     /gene="guaB"
                     /locus_tag="OQQ66_000556"
     CDS             complement(2207..3673)
                     /gene="guaB"
                     /locus_tag="OQQ66_000556"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1903597.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            3835..5205
                     /gene="xseA"
                     /locus_tag="OQQ66_000557"
     CDS             3835..5205
                     /gene="xseA"
                     /locus_tag="OQQ66_000557"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1903598.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5202..5417)
                     /locus_tag="OQQ66_000558"
     CDS             complement(5202..5417)
                     /locus_tag="OQQ66_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1903599.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5486..6958)
                     /gene="der"
                     /locus_tag="OQQ66_000559"
     CDS             complement(5486..6958)
                     /gene="der"
                     /locus_tag="OQQ66_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1903600.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7076..8254)
                     /gene="bamB"
                     /locus_tag="OQQ66_000560"
     CDS             complement(7076..8254)
                     /gene="bamB"
                     /locus_tag="OQQ66_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1903601.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8265..8885)
                     /gene="yfgM"
                     /locus_tag="OQQ66_000561"
     CDS             complement(8265..8885)
                     /gene="yfgM"
                     /locus_tag="OQQ66_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1903602.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(8903..10177)
                     /gene="hisS"
                     /locus_tag="OQQ66_000562"
     CDS             complement(8903..10177)
                     /gene="hisS"
                     /locus_tag="OQQ66_000562"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1903603.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10288..11406)
                     /gene="ispG"
                     /locus_tag="OQQ66_000563"
                     /gene_synonym="gcpE"
     CDS             complement(10288..11406)
                     /gene="ispG"
                     /locus_tag="OQQ66_000563"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1903604.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11433..12446)
                     /gene="rodZ"
                     /locus_tag="OQQ66_000564"
     CDS             complement(11433..12446)
                     /gene="rodZ"
                     /locus_tag="OQQ66_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1903605.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12731..13885)
                     /gene="trmG/rlmN"
                     /locus_tag="OQQ66_000565"
     CDS             complement(12731..13885)
                     /gene="trmG/rlmN"
                     /locus_tag="OQQ66_000565"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1903606.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14035..14466)
                     /gene="ndk"
                     /locus_tag="OQQ66_000566"
     CDS             complement(14035..14466)
                     /gene="ndk"
                     /locus_tag="OQQ66_000566"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1903607.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14615..16927)
                     /gene="pbpC"
                     /locus_tag="OQQ66_000567"
     CDS             complement(14615..16927)
                     /gene="pbpC"
                     /locus_tag="OQQ66_000567"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS1903608.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDCTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(16928..21889)
                     /gene="yfhM"
                     /locus_tag="OQQ66_000568"
     CDS             complement(16928..21889)
                     /gene="yfhM"
                     /locus_tag="OQQ66_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS1903609.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAP
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22089..22934
                     /gene="sseA"
                     /locus_tag="OQQ66_000569"
     CDS             22089..22934
                     /gene="sseA"
                     /locus_tag="OQQ66_000569"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1903610.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(23427..24203)
                     /gene="sseB"
                     /locus_tag="OQQ66_000570"
     CDS             complement(23427..24203)
                     /gene="sseB"
                     /locus_tag="OQQ66_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1903611.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24345..25628)
                     /gene="pepB"
                     /locus_tag="OQQ66_000571"
     CDS             complement(24345..25628)
                     /gene="pepB"
                     /locus_tag="OQQ66_000571"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1903612.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(25806..26006)
                     /gene="iscX"
                     /locus_tag="OQQ66_000572"
     CDS             complement(25806..26006)
                     /gene="iscX"
                     /locus_tag="OQQ66_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1903613.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26018..26353)
                     /gene="fdx"
                     /locus_tag="OQQ66_000573"
     CDS             complement(26018..26353)
                     /gene="fdx"
                     /locus_tag="OQQ66_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1903614.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26355..28205)
                     /gene="hscA"
                     /locus_tag="OQQ66_000574"
     CDS             complement(26355..28205)
                     /gene="hscA"
                     /locus_tag="OQQ66_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1903615.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28222..28737)
                     /gene="hscB"
                     /locus_tag="OQQ66_000575"
     CDS             complement(28222..28737)
                     /gene="hscB"
                     /locus_tag="OQQ66_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1903616.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(28833..29156)
                     /gene="iscA"
                     /locus_tag="OQQ66_000576"
     CDS             complement(28833..29156)
                     /gene="iscA"
                     /locus_tag="OQQ66_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1903617.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29173..29559)
                     /gene="iscU"
                     /locus_tag="OQQ66_000577"
     CDS             complement(29173..29559)
                     /gene="iscU"
                     /locus_tag="OQQ66_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1903618.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29587..30801)
                     /gene="iscS"
                     /locus_tag="OQQ66_000578"
     CDS             complement(29587..30801)
                     /gene="iscS"
                     /locus_tag="OQQ66_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1903619.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(30913..31401)
                     /gene="iscR"
                     /locus_tag="OQQ66_000579"
     CDS             complement(30913..31401)
                     /gene="iscR"
                     /locus_tag="OQQ66_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1903620.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(31671..32411)
                     /gene="trmJ"
                     /locus_tag="OQQ66_000580"
     CDS             complement(31671..32411)
                     /gene="trmJ"
                     /locus_tag="OQQ66_000580"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1903621.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32530..33333
                     /gene="suhB"
                     /locus_tag="OQQ66_000581"
     CDS             32530..33333
                     /gene="suhB"
                     /locus_tag="OQQ66_000581"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1903622.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33478..34332
                     /locus_tag="OQQ66_000582"
     CDS             33478..34332
                     /locus_tag="OQQ66_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1903623.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34523..35803
                     /gene="csiE"
                     /locus_tag="OQQ66_000583"
     CDS             34523..35803
                     /gene="csiE"
                     /locus_tag="OQQ66_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1903624.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35795..36934)
                     /locus_tag="OQQ66_000584"
     CDS             complement(35795..36934)
                     /locus_tag="OQQ66_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1903625.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGAFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37094..37984)
                     /gene="hcaR"
                     /locus_tag="OQQ66_000585"
     CDS             complement(37094..37984)
                     /gene="hcaR"
                     /locus_tag="OQQ66_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1903626.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38120..39481
                     /locus_tag="OQQ66_000586"
     CDS             38120..39481
                     /locus_tag="OQQ66_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1903627.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39478..39996
                     /locus_tag="OQQ66_000587"
     CDS             39478..39996
                     /locus_tag="OQQ66_000587"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1903628.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            39996..40316
                     /locus_tag="OQQ66_000588"
     CDS             39996..40316
                     /locus_tag="OQQ66_000588"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1903629.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            40313..41125
                     /gene="hcaB"
                     /locus_tag="OQQ66_000589"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40313..41125
                     /gene="hcaB"
                     /locus_tag="OQQ66_000589"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1903630.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41135..42149
                     /locus_tag="OQQ66_000590"
                     /pseudo
     CDS             41135..42149
                     /locus_tag="OQQ66_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
BASE COUNT         9951 a        11663 c        10593 g         9942 t
ORIGIN      
        1 actctctgct gtagcagaag cggaacagca aggtaaaaat gaagagcaaa ggcagcatga
       61 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
      121 ccaggtcgcc gctaacaaaa gagcggcaac ggcagcagca aataagaaag ctcgtcagga
      181 taaactggac gccgaagcct ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      241 acgcagctta gagattcaga aacaaaaact ggcgctggca aaagaagaag cccgcgttaa
      301 gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
      361 gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag
      421 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
      481 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacagaggg ttttgttcat
      541 tcatagtgca gggattaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
      601 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
      661 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
      721 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtacc
      781 ggcaggaaca cagtgaacgc ctggctgact ttgttgtaca ggtccgcttt acgcagttct
      841 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
      901 agcacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
      961 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1021 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1081 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
     1141 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaacacttc aacgaaaacg
     1201 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1261 cgatcttctg ccggtacgtg aacaatgttc agaccaaagt gatcgccaaa catatccaga
     1321 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcagattt
     1381 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     1441 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     1501 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     1561 atgtgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     1621 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     1681 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     1741 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     1801 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     1861 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     1921 tgcggcgcac gcggactgtt ttcttcagta gtactttccg ggccgccgga aagaataatg
     1981 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2041 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2101 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2161 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2221 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2281 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     2341 tcaggcccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     2401 ctttataggc tacgcgacct tcgatacctt ccggcactag tttgtcggca gcgttgtcac
     2461 tctggaagta acggtcagaa gaacctttgg acatcgcacc cagagagccc ataccacggt
     2521 aagatttgta agaacggccc tggtagagtt caatttcacc cggagattct tcagtacccg
     2581 ccagcatgga acccaccatt accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     2641 cggagaagcg aataccacca tctgcgataa ccggaatacc tgtgccttcc agcgcttcaa
     2701 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     2761 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     2821 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     2881 gggtttcacg gatacgctgc aaaacgcctt ctgagtggcc gtgggaggag tcgatcagca
     2941 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3001 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3061 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3121 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3181 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3241 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3301 taacaaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     3361 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     3421 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     3481 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     3541 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     3601 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     3661 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     3721 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     3781 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     3841 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     3901 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     3961 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4021 ttccgcaaca gcaaccgccg ggtgaccttc cgtccacagc atggacaaca agttttagtt
     4081 cgcgccaata ttacgctcta cgagccgcgc ggtgactacc agataatcgt tgagagtatg
     4141 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4201 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4261 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4321 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcaccg
     4381 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     4441 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     4501 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     4561 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     4621 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     4681 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     4741 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     4801 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     4861 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     4921 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     4981 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5041 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5101 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagtt
     5161 aaaaacatcc agccagtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5221 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
     5281 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     5341 tccccgcagg atgggcaacg cgcatggccg ttatcctgat caagcacatg ctgacattgt
     5401 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     5461 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     5521 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     5581 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     5641 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     5701 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     5761 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     5821 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     5881 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     5941 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6001 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6061 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcatct tcaatggcct
     6121 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6181 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6241 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6301 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     6361 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccact tcaaattgcg
     6421 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     6481 gcagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     6541 tttcacctaa gccaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     6601 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     6661 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     6721 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     6781 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     6841 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     6901 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     6961 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7021 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7081 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7141 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7201 cgacattaat ccagtgcaga taaccttcac tgtcaccgac caccaggttg ccatcataca
     7261 gcaccggaga agtcagcagg cgatgcaaca gatcgctttg tgtccacagc gtaacgccgc
     7321 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     7381 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     7441 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     7501 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     7561 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     7621 cgccccccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     7681 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt
     7741 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     7801 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     7861 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc acaccgccag
     7921 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     7981 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8041 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8101 cggaagtact ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8161 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8221 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8281 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     8341 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     8401 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     8461 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     8521 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     8581 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     8641 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     8701 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     8761 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     8821 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     8881 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     8941 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9001 tcacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9061 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc tgtaattcat
     9121 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9181 ggtatatatc gacaatagga tcggctttaa attccggatt aacggcctgt actaacaata
     9241 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt
     9301 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
     9361 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
     9421 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
     9481 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
     9541 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
     9601 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
     9661 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
     9721 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
     9781 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
     9841 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
     9901 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattga
     9961 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10021 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10081 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10141 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10201 aaatgaaccc tcaacgcttc cctcaatgtt tggggagcca tgcgggttca atcatacacg
    10261 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10321 cgcttcatcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    10381 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    10441 gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    10501 aatagaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    10561 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt tgatccctcg
    10621 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    10681 ggccgccagc gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    10741 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    10801 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    10861 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatgacg
    10921 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    10981 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11041 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11101 gacgccgtat tccgctactt tcagcgcaat gcgatagtcg aagtggatgt cagccaccag
    11161 cggcacgtta acccgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11221 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11281 ttcgacatct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    11341 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    11401 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    11461 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    11521 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtacgg tgcctggccg
    11581 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    11641 tcagtgacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    11701 accggcgtgg tcacgccagc ctgatcggtt ggcaacggcg cagcaccatc tggcgttgtt
    11761 gctgccgttg gtgccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    11821 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    11881 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    11941 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12001 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12061 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtca
    12121 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatcg gcgcaacttt cgcagcccgt
    12181 agtggagcct gcttttccag ccctagcagc agttcttctt ctggaatatg taccagacgc
    12241 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12301 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    12361 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    12421 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    12481 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    12541 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    12601 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    12661 attgcaactt aatacttcat tcataaaaga ttacgccacg gtacataaag taaccgtggc
    12721 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    12781 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    12841 caccacgagt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    12901 ggtcgatacg gctgttcgaa ctgcgtccat acggcgcgcc cgggaacggg ttccacggga
    12961 tcaggttgat cttacacggc gtatctttca gcagctccgc cagttggtgc gcgtgttcag
    13021 tgccgtcgtt aacgtggtca agcatcacgt attcaatagt gactcggccc tgattggcgt
    13081 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13141 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13201 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13261 tgacgcgacg tttagacagg ccaaaaccga aatcatccag catgatttcc atcgccggaa
    13321 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    13381 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccga
    13441 taatttccga cacccgcagg ttgcgattaa agccctgctg ggcggtggaa cagaatttac
    13501 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    13561 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    13621 atgaacgctg ttcttcaacc acttccggtg cgcggatttc cgccacctct ttcagtttgc
    13681 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    13741 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    13801 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    13861 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    13921 cctggagggt tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    13981 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14041 ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg atttcgcgag cggcagattc
    14101 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14161 agtaccagcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14221 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14281 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgtgcct gttcaacggt
    14341 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    14401 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    14461 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    14521 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    14581 aatgagtttt agcaacaatc agggacaaaa cagactattg catgacaaat ttcactgtcg
    14641 cgatttgccc cacgtcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    14701 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    14761 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    14821 taatcgcgcc atcgcgaacg cctgtcagct gcagcgggag ctgggcatag tgaccgtatg
    14881 gcgggcaact tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    14941 agggttccag cggtagcggc cagacattta tcatttcttg tcgcgcttgc gggcaatcag
    15001 cggcaacacg tttgccattt tcatccagcc agatggggaa acgaatgcca ttaatgcctt
    15061 cctgctccgg cagtaataga gttggcggct gactcccgtc cagcagccag gttgccaggc
    15121 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15181 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15241 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15301 gcgtgcagtc cggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    15361 cccaggcgtc acgataacca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    15421 gcaaggcact atccggcaag ggttgcgctt catcagccat aatccggcga atgatccacg
    15481 ccgcccccga cgacattaaa ggccattcaa gcagcggatc gtcaggctgt aagcgcaatt
    15541 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    15601 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc accgttgggc aaatataacg
    15661 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    15721 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    15781 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    15841 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    15901 ccgatcctgg cgatcggatc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    15961 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16021 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16081 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16141 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16201 cttcccttga ctcttttacc tgctcacggg accacacacc ttgcacggcc atccgttcga
    16261 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcgc agtcggctgg
    16321 gcgcttgcgg taacaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgatt
    16381 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    16441 ggttaagata caagatcaga atttcacgct tagacaaatg ccactccagt tgcaacgcgc
    16501 gccagagctg gagaattttg ccaccaaatg ttttggggtg aggatcaagc agacgagcaa
    16561 cctgcatagt gagcgtgctg ccaccagaaa taacccgtcc cgaagtgaga tcttgccatg
    16621 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag cgatcttcat
    16681 aattgatcag cgcttcaagg taacgtggag acacatcttc gattgttacc ggataacgcc
    16741 agatgccatc agcatcggcg aagcgccaga gcggcgtacc atcctgcgcc acgaccactc
    16801 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    16861 gaaaaaggag aaatggcaca gctgccagcg ttatccagca gccgcgtttg gttaacaagc
    16921 gaagcattta cggtcggaca atcagcagat cttcagccgc gccggtcgcc cgccattggg
    16981 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17041 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17101 gatcgtcacg gaactcaatg tgcttaatgc tcgcctgctg catctggttc agtaagtttt
    17161 gcacttcgtc accactttgc tccaggctgg cgctaccgtt cgccagattc tgattttcca
    17221 gttccagacc cgcaggcagc agatccacga ctaacgcatc cggcacgctg ttactggctt
    17281 ttacctgcaa ccagaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    17341 taccatcagt accaagaata tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg
    17401 cggattgcgg ataaccgctg gcatccatac gcaaccataa cggctgatcg ccactgtttc
    17461 tcacctgcaa ggtggcaagt tgatcgctat tcagattgct gtttagcgtt ttctcgcctg
    17521 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    17581 taggggcagc caggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaacg
    17641 cctgctgcga aagtgtgttc agcaaagtgt actgctcatc gggtagcagt ttattttctt
    17701 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    17761 tccgctcatc actatttctc ggcgttttca gcgccagcac aatcgcttct tcaccacgcg
    17821 ttgcatcacc catggttttc agcgcaatgc caagttgcag cagcggtaaa ccagaagcgg
    17881 catctgcacg atgctcccag atttcacgca gcgcacccag tggtgctttt tgctgacggg
    17941 ccagcaccag cgcagcgtaa gactgaacgg cgaatttact ggctttgaga ttatccgcgt
    18001 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18061 taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18121 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18181 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18241 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18301 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    18361 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    18421 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    18481 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    18541 tatgctgatc ggcaacggtt tcacccggta acgctaaccc gctaatggtg gcctgaattt
    18601 ctccatcgcc ataacccggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    18661 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    18721 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    18781 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    18841 ctatcacttt gctttcgttg ctaccgaaat catctgccgt ccaggcttgc gccatgacgc
    18901 gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    18961 gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg attgaccagc ggtttaccac
    19021 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19081 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19141 cctgccacgg atctggcgtg acgtagtcag taatattcag cacaccacta tcaacggcag
    19201 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19261 cggttaatgg ttgattggga cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19321 ggttttcatc gccaagcggc agatgcaaca caccaaccgc gcgttttggc gtcgcggagc
    19381 gagatttatc gctaggacgt accaccagcg tacttaaata cagatcatga cgattccagg
    19441 ttttatcgac cggaatcgtc agatccagcc cttgagccgg aacatcaatc tcttgccacc
    19501 acagcggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcga
    19561 tatgcaactt aatggtgtcg ccagggcgat aactggcttt atccagtttc agcgtgacac
    19621 ggtcgggtcg cactgcgccg ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga
    19681 aacgaacact actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    19741 ctaccggaaa actgaccttg ccggtttcgt ccgctttcag atccagagtt tgttcatttt
    19801 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    19861 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    19921 cttgtgcatc gctataaacg atgtcaaaag cggcgttact gccttcatca acaatgggct
    19981 gtttcaccgt gctgtcagtg cgataatcgt aaaccgattt caaggcgaac tgcggacgga
    20041 gccccggcaa tgcattggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20101 cgcccgattc cagcaaactg ccctggaaaa taacctgcaa tggggaatgc gtttccttcc
    20161 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20221 ccagcgtgcg ggaaaggttt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20281 acacagcctc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    20341 gcgcaccgta caggtagtat cccaccacgg agaatttcac ttcatcattc ggcgtaagcg
    20401 gggttttctc accggtcaga ttcagcgcca tacgctctgg cataaaatct tcgacgtgga
    20461 aatcccacat ccggtactgg ttatcgcccg tgttggcgcg aatatgccac ataccggttg
    20521 ccgcattgct atcgagtggc caggtaaagt ggtagaggcc attctccggc tgactaacga
    20581 cgctcctgag tacctgcccg tcaggtttaa tcacgtctaa cttgatgggt tgatcgggca
    20641 atgctttacc gtctgcatcg cgcagtaaac cattgaggat taccgtttca cctgggcgat
    20701 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    20761 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    20821 tacgtgccag cagtaatgcc gcatttttat cattttccag ctgcacatgc ccctgagcgt
    20881 cactggttgc ctgagtcaga gtctgccctt tctcatttaa taaagagact tcaattcctt
    20941 gctgggccgc gccgttttcc agactttggg taaagatatc cagacgattg tgataacggt
    21001 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21061 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21121 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21181 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21241 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21301 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    21361 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    21421 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    21481 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtag ctctttaaga ttatctgaca
    21541 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    21601 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    21661 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    21721 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    21781 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    21841 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatc cctgccacaa
    21901 tggcccgtta gcaacgtcga ataattatta tgcgtgagaa tttacgttat gtaattcagt
    21961 atcacgccca tacaatccga attttagtga gaattgtggc tcagacagcc aaatcagcct
    22021 ctttcgcttg tcgcttttca ctaaacatcc gacaatttag cctaaccccg gcaaaaatgg
    22081 agatgcctat gtccacgaca tggtttgtag gagccgactg gctcgccgaa catattgatg
    22141 acccggaaat tcagattatc gatgcccgca tggcgtcgcc tggacaggaa gatcgtaacg
    22201 ttgctcagga gtatctgaat ggacatattc ccggcgcagt gttttttgat atcgaagcgc
    22261 tttctgatca cacttccccg cttccgcaca tgctgccgcg cccgaaaacg ttcgccgtgg
    22321 cgatgcgtga attaggcgtt aaccaggata agcacctgat tgtctatgac gaaggtaatc
    22381 ttttctcagc cccacgagca tggtggatgc tgcgcacctt tggtgtagag aaagtgtcga
    22441 ttctgggggg tggacttgca ggctggcagc gcgatgatct gctgttagaa gaaggtgcag
    22501 tagagctgcc ggaaggagag tttaacgccg cgtttaatcc tgaagccgtg gtgaaagtaa
    22561 ccgatgtatt attggcaagc catgaaaata cggcgcaaat tattgatgcc cgcccggctg
    22621 cacgttttaa cgcagaagtt gatgaacctc gcccaggttt acgtcgcgga catattcccg
    22681 gagcactgaa tgttccgtgg acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg
    22741 aactggatgc gatatttttt ggtcgcggcg tcagctacga caaaccaatt atcgtcagct
    22801 gcggctctgg tgtaacggca gccgtggttt tgttagcact cgcgacgctg gatgtgccaa
    22861 acgtgaaact gtacgacggc gcatggagtg aatggggcgc gcgggcagat ttaccggttg
    22921 agccagtgaa ataagtattt tacaggcaat aaaaaaccgc cgaatttggc ggttttttat
    22981 tgctggtctg gttcgcggcc tttccagcag gttgtattac cgtagtaatg caagcgcgtc
    23041 tcagcggaga caatactcgc cagtaactct ctttttgtca agcaaaagag agttattatt
    23101 gttctgttag tatattatcc actgcggccc tttccgccgt ctcgcaagcg ggcggtggct
    23161 taaggaaagg atgttccgcg accgttagag caagcgcgtc tcagcgcttg ctatcgcggc
    23221 aatatcgcca gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa tgaagatacg
    23281 gtgcttctgt atcgtactta ttgtttctgg tgcgctgttt tcccaagtaa ataataaccg
    23341 gagtctctcc ggcgacaatt tactggtggt taacaacctt cagagcagca agtaagcccg
    23401 aatgccgccc tttagggcgg catattttag attatccggt tttgtttaaa gtcacgcaaa
    23461 aaacctcccc agcgacgttc atagaatggc acaatatgtt cggtaataaa gtggctaatt
    23521 cctttttccc cttttttcac ctgacaaata tcgattggtt catcgccagg taatgtatcg
    23581 gtcgctacac ttcccgtcgc ctgaataatt tcttcgatat caccatcggc ttcaatgcca
    23641 ataaataaat taggctgtgc ctcttcgttc tctttaattg aacaaataaa agcacgcttc
    23701 accggcttaa tggttttaaa taaggtggtg agtgaatcaa tcatttgtgc tggcggctct
    23761 gcaacttccg ataatatcag cgattcaccg ccttccagga tttcctggct gctcagcgga
    23821 tttccctctt caccaatcaa caaactgatt tcacgcggca taaattcttt accggtcggc
    23881 agtttggcat taaggaagag cgtttcgcca agggtcatct caaacaatgt gcgaacgggc
    23941 attacgacaa atgcctgttc gtcttcaacc gcctgttgaa gcgcttctaa cgaggtgaaa
    24001 aaaggaatga cgctggtgcc gtcttctttt tcccagtgct gtaaatcaag cgcactatct
    24061 tcaaccacag cctcgccctg cgccgccgta ccaggcaccc agacggtgga ttccagtaga
    24121 gtacggaaaa aggccgggcg gtgtgccggt tcggttgctg ctttttccag caggtcttca
    24181 agttcgtttt ttgtttcgga cataagaacc acaattcatt caacgttcgg ggcgcaaatg
    24241 cctgatgcgc tacgcttatc aggcctacaa gggattcgca atttgttgaa tttgcagaat
    24301 ttgtaggccg gataaggcgt ttacgccgca tccggcatat tagtttacgc cgttaacaga
    24361 ttagctatcg tgcgcacacc aagtcccgta gcgcccgcag accactgttc aaccggcgct
    24421 ttacggtaag tcgccgagca gtcgatatgc agccagcctt gctgatagtt ctcaacaaag
    24481 tgcgacagga agcccgccgc cgtgctcgcg cctgccggat acgccgcgct tccggtattg
    24541 ttcagttcgg caaagttaga cggcagctgg ctgcggtgga attccgccag cggcagacgc
    24601 cagaatggtt cgttctcttg tgaggcactc gccagcaaac gaccggcaag cgcatcgtca
    24661 aaactgaaca gcgcgtgata atcattaccc agcgcagttt tcgccgcccc ggtgagggtc
    24721 gccgcatcaa tgatcatttc cggtttctgc gcactggcat caatcagacc atcggcaagc
    24781 accagacgcc cttcggcatc agtgttcatc acttcaactt ttttaccgtt gcgataggtg
    24841 atgatatcgc ccagcttgaa cgcattgccg ctaatcaggt tatccgcaca gcagaggaac
    24901 agcttcacgc gcttgttcag tccgcgcgta atggcaaatg ccagcgcccc ggtaaccgtt
    24961 gccgcgccgc ccatgtccga cttcatcgag tccataaacg cagtctgttt gatgctgtag
    25021 ccgccggagt caaaagtgat acctttacct accaggcacg cgtacactgg cgcttcttta
    25081 tcgccagttg ggttgtaatc cagcgccagc aataccggag aacgttctga agcgcgtccg
    25141 acggtgtgca gccccatata accttgctca cgcagatctt cgcctttggt gatccgataa
    25201 gtcacacgat cgcccgcgac gttgctgatc agatcaacag cacgctgtgc cagttgcgat
    25261 ggtcccaatt cttctgccgg tgcgttgatg gtgtcacgca cccagtcgat gatcatcagg
    25321 cggttatcca gttcctggcg ctgggcatcg tccagatccg gccactccac tttacgcgtg
    25381 cctttcgggg ctttgtaacc ttgccagaat gcccagcagc gatccgcatc ccagccttca
    25441 ccgcttaact gaacatgctt gatgcccaga ccgtcaatct tgcgcgccgc acgctggatc
    25501 agccccagat cgtctgcccc gttcaaatgc agggtaatgc cgtcattatt aatgctgtaa
    25561 gttgcttttt ctccccagcg cgcgtcggca ggttgggtag agagggtaat cttcatcgct
    25621 tctgtcattt tagttatcct tctttgtaaa agggccgcca gttggcagcc ccaggatttt
    25681 tccgctaaat gattcgcgtt gcaggaaggc ggcaagtgag tgaagcccca ggagcataga
    25741 taactatgtg actggggtga acgagcgcag ccaacgcatc tgcggcgtga agcatgacgc
    25801 ggaaattatt cggcctcgtc cagccagact aacaaaatcg cttcgaggat tttctcgttg
    25861 gatgcctgcg ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg catatcggtg
    25921 aatcgaaccg ttttcggatc cagatcagga tacgcatcgt acagtgcttc gccaatttcg
    25981 cggctatcgg tccacttaag tcccatacta acctctgtta atgctcacgc gcatggttga
    26041 tagtgtaacg cgggatttcg actactaaat cttcgtcggt aacgcgtgcc tggcagctta
    26101 aacggctttc gggctccagt ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc
    26161 tttctggcag cgagtcaaaa ccttcacgaa cgatgcagtg gcaggtggtg caagcacagg
    26221 atttttcaca ggcgtgttca atctcgatac cgttacgcag cgctgcgtcg agaatggttt
    26281 caccgctatt agcttccaga acagcgccat cagggcagag gtcctgatga ggcaaaataa
    26341 caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac
    26401 tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt
    26461 tcgatggcgt caacatcatc gccctgagcc acttcactca ggtgagcggc agcattgtca
    26521 atgacctgac gctctgcggc gcttaacagc gcggcatcag cagccagcgc gccgtgcaga
    26581 ctttccagca cacgcgccgc ttctactttt tgttctgcca gcatacgggc ttttacgtcc
    26641 tgctcggcat agctcattga atctttgatc atcgaagcga tttcgctatc ggtcagaccg
    26701 taagaaggtt tgacctgaat agacgcctca acgccggtgg atttctccat cgccgtcacg
    26761 ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc gccagccggt
    26821 agcgccggaa taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg
    26881 cgctcaccct gcattacatg gatagacatc gccgtctgac catctttaaa ggtggtgaaa
    26941 tcctgagcgc gggccaccgg aatagtggta ttacgcggaa tcactttctc caccaggccg
    27001 cccatcgttt cgaggcccag cgacagtggg atcacatcaa gcagcagcat ttcgctgtcc
    27061 ggcttgttac ccaccagaat atccgcctga atcgccgcgc caatagcgac gactttatcc
    27121 gggtcgatgg aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc acgcaccagc
    27181 ggcacgcgag tagaaccgcc aaccatcacc acttccagca cttcatcagc ttctacgccc
    27241 gcgtctttca gcgcacgacg acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt
    27301 tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac ggtcacggag
    27361 tccgcatcgc tcagcgcgat tttggctgca atggcggcat ccagcagttc acgctgaacg
    27421 cggttatcgc tacgatcagg aatgtccgcc tgctcgcgaa tgtaatccgc cagcagatgg
    27481 tcgaaatcat cgccgccgag cgcggaatca ccgccggttg ccagcacttc aaacacgccg
    27541 cgacttaagc gcagaatgga aatatcaaac gtcccgccac cgaggtcata aacggcgatc
    27601 acgccttcct gaccggaatc cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt
    27661 aagcgcagaa catgcaaccc cgccagacgc gccgcgtctt tggtgccctg acgctgggca
    27721 tcgtcaaagt acgccggaac ggtgataacg acaccatcca gctcgcctgc cagggcttca
    27781 gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc
    27841 cccgccgccg tttcaatcat tggcaggccg ttttcgctgg cctggaattg ataaggcaaa
    27901 tgcggatagc gttgctggat atcagccagc gagcgtccca tcaggcgttt aacagaacta
    27961 atggtgttgg cggtatcgag cgctgcgtta gtacgcgcgt cataacccac cgaatgccct
    28021 tgctgttgat agtgaacaac agatggcagc aggtgacggc cttcatggtc ggctaacgtt
    28081 tcggcctgac cgctgcgcac tgtcgctacc agcgagttgg ttgtgcccag gtcaataccg
    28141 gccgccagac gacgctgatg cggcgcagca ctcaaaccag gttcactaat ttgtaataag
    28201 gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt gttcggcact
    28261 gcttcgcagt ttatcgagaa aacgcagctt acgcacggta tccgccgccg cgtcccacgt
    28321 ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct ttttcacacg
    28381 tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt ccagttcttc
    28441 gcgcagctcc agctgttcca tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc
    28501 gagatcaaaa ccgtgcaaag aaagcaaata ttccgcgcgc attaacggat gacgcagcgt
    28561 ttgccaggcc tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct
    28621 tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac gcaggctcag
    28681 tgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt aatccataac
    28741 attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc
    28801 aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc gccgcaacca
    28861 cactcatctt taacgttcgg gttggtgaat ttaaaccctt cattcaggcc ttcttttacg
    28921 aagtccagct gcgtaccgtc cagaaattgc aggcttttgc catcgaccac gactttcacg
    28981 cctttgtctt caaacacgat gtcttccggc gtcggttcgt caacaaattc cagtacataa
    29041 gccatacctg aacatccgga ggttctcacg cccagacgca ggccaaaccc tttgccgcgg
    29101 ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc
    29161 aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg cggctttgat
    29221 cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa gttcttcagc
    29281 aatatcggtg tttttgatcg cctgcgcttc gacgagagac ttccctttca cccattcggt
    29341 gaccagggag ctggaagcga tagcagaacc gcagccgtaa gttttaaaac gcgcgtcttc
    29401 aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac aggccggtgc
    29461 acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg
    29521 attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc ctgattccga
    29581 taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct tgtacatttc
    29641 ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact cgatggtgta
    29701 gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc tatgtgccag
    29761 ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca
    29821 ggcggaacct gaagaaactg cgaggtcttt cagcgccata atcagcgact caccttcaac
    29881 gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta
    29941 aacttcttcg atatctttga tgccgttcca cagacggtta cgcaggccgc gcagacgttc
    30001 catctcggtc gccatctctt cttttgcgat gcgataggcc tcgcccattc cgacgatctg
    30061 gtgaacaggc agagtgccgg aacgcatacc gcgctcgtga ccgccgccgt gcatttgcgc
    30121 ttcgatgcgt acgcgcggtt tacgacgtac atacagcgca ccgatacctt tcgggccata
    30181 gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcaggt cgataggcag
    30241 tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac ggcacatttc
    30301 gccgatagcc gcgatatcct gcaccacgcc gatttcgtta tttacgtgca tgatggaaac
    30361 aaggatggtg tcgtcacgca tcgctgcttc aagttctttc aggtcgataa tgccgttacg
    30421 ctgcggtgcc aggtaggtga cttcaaaacc ttcgcgctcc agctgacggc aggtatccag
    30481 taccgctttg tgttcggttt tgctagtgat gatgtgcttg ccttttttct gataaaagtt
    30541 ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa agacgatttc
    30601 acgcggatca gcgccgacca gatcggctat ctgattacgg gcgatatcta ccgcttcttc
    30661 agcctgccag ccgaaacggt gagaacgaga ggccgggtta ccaaaggttc cgtccatcgt
    30721 catgaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg cggagtagtc
    30781 gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat gcaaggaatc
    30841 aggctaccgg ctggatgtac gaccgtgttt acggagtatt tagcactccg gcctgattct
    30901 gaattctttt tattaagcgc gtaacttaac gtcgatcgcg tcttgtgtgc gggtgcgtgg
    30961 cgcgtcgtga gtatgctgac gaccagacac atccagcact tcctggttat taaccagttc
    31021 gcctaaagta atgttgttga gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc
    31081 gtgggtcagg catttatcgc cgccctggca gccgccttta ccctgacaac gggtggcatc
    31141 tacagattcg tcaacggcgc taatcacttc gccaacggcg atgctgctgg catctttgcc
    31201 taacagataa ccaccgcctg gtccacgtac gctggaaacc agaccatttt tacgcagacg
    31261 ggaaaacagt tgttccagat aagaaaggga aatgccctga cgttcggaaa tatcagccaa
    31321 cggtaccggg cccgcttcag agttgagcgc aacgtcaagc attgcggtca cggcatagcg
    31381 ccctttagat gtcagtctca tgtcttactt cacctcaaac tcgcccctgc ccggggtttt
    31441 ttattgtaaa agtgggggta ttgaatagca gggtcaagtc tgacattccc gagtaaattg
    31501 gtcaactatt tacttgactg atttagtcgg gtatttaacc ttcagtgcca tttttttatc
    31561 gtggcgtttg tacctgttgt cggatgcggt gtgaacacct tatccgacct acggttctgt
    31621 acattgtagg cctgataaga cgcgttaagc gtcgcatcag gcaactactg ttattcggcc
    31681 ttgttacctt tattctgctg ctcaatagaa gccagaatcc cgcgcaggat attcaattcc
    31741 tggctttccg ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac ctgccccgga
    31801 tggttttcac ggataaaacc ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc
    31861 tccagatcat cgaccagcgg atacggcgtc tcttcatgct cgacctgctc gccgttttcc
    31921 tgagttgcca gccaggccat gcgcacttca taggcaataa cctgaaccgc catcgccagg
    31981 ttcagcgagc tgtattccgg gttagccgca atcgcgacat gataatggca tttctgcaac
    32041 tcttcattgg tcaagccgac gcgctcgcga ccaaacacca gcgccaccgg ggtatttgcc
    32101 gcttcagcga cgcttttcag gccgcattcg cgcgggtcga gcatcggcca tggcagcgtg
    32161 cgggaacgtg cgctggtgcc caccaccaag ctacaaccgg ctaacgcttc gtcgagcgta
    32221 tcgacgatgt gagcattacc aatcacatcg ctggcccctg ctgccagggc aatcgcctgg
    32281 gagtcgggtt tcaccagtgg attaaccagc cacagattgg ttaatcccat tgttttcatg
    32341 gcacgggcaa cagaacccat attgccggtg tgtgacgtct ccaccagcac aattcgaata
    32401 ttttgcagca ttgtctttct tcatctaaag attattcacg catcttatca taaaacgaag
    32461 acagatgccg atctcgctgc tatactctgc gccgttttcc cgttctttaa catccagtga
    32521 gagagaccga tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat
    32581 ttaattgcca aaaactatga aaccccggac gctgtagaag cgagccagaa aggcagtaac
    32641 gatttcgtga ccaacgtaga taaagctgcc gaagcggtga ttatcgacac gattcgtaaa
    32701 tcttacccac agcacaccat catcaccgaa gaaagcggtg aacttgaagg tactgatcag
    32761 gatgttcaat gggttatcga tccactggat ggtactacca actttatcaa acgtctgccg
    32821 cacttcgcgg tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac
    32881 gatcctatgc gtaacgaact gttcaccgcc actcgcggtc agggcgcaca gctgaacggc
    32941 taccgtctgc gcggtagcac cgctcgcgat ctcgacggta ccattctggc gaccggcttc
    33001 ccgttcaaag caaaacagta cgccactacc tacatcaaca tcgtcggcaa actgttcaac
    33061 gaatgtgcag acttccgtcg taccggttct gcggcgctgg atctggctta cgtcgctgcg
    33121 ggtcgtgttg acggtttctt tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag
    33181 ctgctggttc gtgaagcggg cggcatcgtc agcgacttca ccggtggtca taactatatg
    33241 ctgaccggta acatcgttgc tggtaacccg cgtgttgtga aagccatgct ggcgaacatg
    33301 cgtgatgagt taagcgacgc tctgaagcgt taatgactca ggcgggtgat gtcactcacc
    33361 cgccctcgcc tttcaggcgc tattccgaaa tacttcctca ccgctttact ttctttcgtc
    33421 actctcccac catctttccc cgattaatgg atgataaaga agtcacaagg aaaggttatg
    33481 gcgctgccag tgaacaaacg cgttctcaaa attctgttta ttctctttgt tgttgccttc
    33541 tgcgtttatt tagtgccgcg cgttgccatc aacttcttct attatcctga cgataaaatt
    33601 tacggtcccg acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt
    33661 ctgcaaggct ggtttattcc ttcttcgacg ggccctgctg acaatgccat cgcaaccatc
    33721 attcatgctc acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta
    33781 cccgagcgta atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc
    33841 acgccgtccc aggccggatt gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat
    33901 cgcagtgatg taaacccaca acggctggtg ctgttcgggc agagtattgg cggggcgaat
    33961 attcttgctg ttattggtca ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc
    34021 acatttgcct cttatgcaac catcgccaat cagatgatcc ccggcagtgg ctacttactt
    34081 gatgagagtt acagcggcga aaattatatc gccagcgtca gcccgatccc gcttttactc
    34141 attcacggta aagctgatca cgttatccca tggcagcaca gcgaaaagtt gtatagcctg
    34201 gcaaaagaga caaaacggtt gatcctaatc ccggatggcg aacacattga tgctttttcc
    34261 gatcgtcacg gcgatgttta tcgcgaacag atggtggact ttatccttag cgcgttgaat
    34321 ccgcagaact aacccatgat cgctagcacg ataatcattc acaaaaccac cttaagacat
    34381 gctaatccac tggtcagaac agtttaagat gagaaaaatt ctgtgacgct tgccaacatt
    34441 tctgatgatt agcattccct tcgccattta cctgagcaaa ctttagctat tcttatcaat
    34501 tatgcttatg ggagatctac agatgatgcc tacgcttgct ccaccatctg tcctttcggc
    34561 tccccagcgc cgctgccaga tcttgctgac gctctttcag ccggggttga ccgccaccat
    34621 ggcaaccttc agcgagctta atggtgtgga tgatgatatt gccagtcttg atatcagcga
    34681 aacaggacgg gagatcctgc gctatcatca actcacactg acgactggtt atgacggtag
    34741 ctaccgggtt gaaggtacag tgcttaacca acgtttgtgt ttatttcact ggctacgacg
    34801 tggtttccgt ctgtgtccgt catttattac cagccaattc acccccgccc tgaagagtga
    34861 actgaagcgg cgcggaattg cgcgtaactt ttacgacgat accaatctac aagcgttagt
    34921 gaatctctgc tcccgacggc tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg
    34981 tctgtatctg caatattgtt tgctgcaaca ccacgctgga attacgcctc agtttaatcc
    35041 gctccaacgt cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg aaattggacg
    35101 ccactggcag cgtcgggcgc tccagcctgt accgcctgat gagccactgt ttatggcatt
    35161 acttttttcc atgttgcggg ttcccgatcc attgcgtgat gcgcatcagc gagacagaca
    35221 attgcgtcag tctatcaaac gtctggtaaa ccattttcgt gagctgggaa atgttcgttt
    35281 ttatgatgaa caggggttat gcgatcagct ttatacccac ctcgcccagg cgttaaatcg
    35341 cagtttgttt gccatcggta ttgataatac cctgccagaa gagttcgcca gactgtaccc
    35401 acgcctggtg cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat acggcgtcca
    35461 tctttctgat gaggaaagtg gtctggtcgc ggtgattttc ggtgcctggc taatgcagga
    35521 aaacgatctg catgaaaaac agattattct tcttaccggg aatgatagcg agcgagaagc
    35581 gcagattgag cagcagttac gcgaactaac gttactgccg ctcaacatta aacatatgtc
    35641 ggtaaaggca tttttgcaga caggcgctcc gcgcggcgcg gcactgatta ttgcgcctta
    35701 taccatgccg ttaccgctct tttcaccacc gctgatctat acggacctga cactgacaac
    35761 acatcaacag gagcagatcc gcaaaatgct cgaatcagca tgagggaaca actttcgggc
    35821 gcaaaaacat tgccggaagc gccaccagcg ccattaccca gaacacgccg tggcccagat
    35881 attgatacag gaaaccggcg aaaacggtca tgatagctat actgccgccc atcgcgacgg
    35941 cagagtaaac cgcctgtaaa cggatgactt cgctaccctg gcgagcggca atatagcgca
    36001 tggcggccag gtggcagacc gtgaaggcgc cgcaatgcag aatttgcacc actatcaacc
    36061 acggcaacgc cgtagttgct cccataatgc cccagcgcac tacgccgcaa atcgccgaga
    36121 tcaacagcat atcgcgtgca ctacaacggc ggaaaagttt attactcagc gcaaagataa
    36181 tgacttccgc caccacgccc agcgaccaca aataccccac cgccgaggcc gagtagccag
    36241 ctgcctgcca gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc tgcaataaac
    36301 aaacgcaggc cagaaagcgc cagttctgaa gaaccagcgc caaccacgct gaccaaccgg
    36361 tgctctcctg ctggcggctt gccccttgtg gctgaatcgt cggacggatg agaaagccga
    36421 gcagcataga tgccacgccc aatgttaaca gcgcgaggat cacctgataa tcaaacatag
    36481 tgaccagttt gcccgtcagc gccgagccaa tgacaaacgc caccgagccc cacagtcgca
    36541 ctttgccgta atcaagcggg aactgctttt gccacgtatt cgccagtgca tcggtcaacg
    36601 gtaccagcgg tgagaaaaag aggttaaagc caatcatcac cagcatcagc cacgctacgt
    36661 gcgcccccgc ccagaaggcg acagcaaaga gaagtgtcat cattgccagc acgcgcaagg
    36721 cggaaatcag gcgagaagga tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac
    36781 gggcaaccag acctgccccc aataacaggc cgatggtttc tggcgttaaa ccaatccctt
    36841 taagccagac gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc
    36901 cgagcgccaa ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag
    36961 tctggggtta atcgaatgtt ggtgcaagtt gaaaaataag aatattcgag cctgcttccc
    37021 gatgtctgta acgccagttc acaagcacta aaaaactgcg atccgcgacg catgttgcaa
    37081 aaataatgcg taattatgcc gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat
    37141 cgcgtaatgc cggcttcatc tcccctttct tccacgccat cagtaaagca atggaaggca
    37201 catttccggc aatgggacga aaaacaacct gtccggtatt aaaattattc atataaccgg
    37261 gtatcaaagt gacgcccagc cccatgccca ccagattcat ggtcaccaga atattcgttg
    37321 ccacctggac gatatttggc tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg
    37381 caagcgaacc ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg
    37441 cggtgatctc tttttcatgt gctaaagggt gatcaaccgg taacacaacc actaatggtt
    37501 cgtcaaaaag ctccagataa tcaatctccg ggctataaac aggatggcgc attaagccga
    37561 cgtcgagttc accacggcga attttttcct cctgttgcgt ggtgattaaa ctcaccagct
    37621 caatcaaggt gtctggctgt ctgagacgaa acatcggtaa tacttttggc agtaaattca
    37681 cttccgccga tggcacaaag ccaatggtta attgtctgtc ttcctgaaca gttttccgcg
    37741 cccgtaattt ggcattttcc gcttgttcga ggattgccag cgcgtcctgg agaaaacatt
    37801 ctcccgccgc cgtcaacgcg actttgcgct tatccctcac cagtaacgga acaccgacac
    37861 agttttcaag atcgcggatc tggctgctta acgaaggctg tgaggtatgc agtttttccg
    37921 ccgcacgggt aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt
    37981 ccatcacctt ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta actatttcac
    38041 atattagcaa ccaaccagca acatccttat ggcacaaaaa tagaaggtca atacatctta
    38101 tctttcagga ttaaaaaata tgaccacacc ctcagatttg aacatttacc aactgattga
    38161 cacccaaaat ggtcgggtca ctccgcgtat ttataccgac ccggatattt accaactgga
    38221 gcttgagcgt attttcggtc gttgctggct atttctcgcc cacgaaagcc agatcccaaa
    38281 acccggtgat ttctttaaca cctacatggg agaagatgcg gttgtcgtag tgcgtcagaa
    38341 agacggcagc atcaaggcgt ttctcaacca atgccgccac cgggccatgc gtgtgagtta
    38401 tgcagattgc ggcaacactc gcgcctttac ctgcccgtat cacggctggt cttatggcat
    38461 taacggcgag ttgatcgatg taccgctgga acctcgcgcc tacccacaag ggttgtgtaa
    38521 atcccactgg gggctaaacg aagttccttg tgtggagagt tataaagggc tgatttttgg
    38581 caactgggat accagcgcac cgggcctgcg tgattacctt ggtgacattg cctggtatct
    38641 ggatggcatg ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg
    38701 ggtgatcaac tgtaactgga aatccccggc agagcagttc gccagtgacc aatatcatgc
    38761 tctgttcagc catgcttctg ccgttcaggt attaggggcg aaagatgatg gcagcgataa
    38821 gcgtctcggt gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca
    38881 atttggtcag gacggtcacg gtagcggttt cttctttact gaaaaaccgg atgctaatgt
    38941 ctgggtcgat ggcgcggttt ccagctatta ccgcgaaacc tatgccgaag cagaacaacg
    39001 tttaggtgaa gttcgcgccc tgcgcctggc gggtcataac aatatattcc ccacgctttc
    39061 atggctaaac ggcactgcca cactccgcgt ctggcatccg cgcggccctg atcaagttga
    39121 agtgtgggcg ttctgtatta ctgacaaagc cgcctccgat gaagttaaag ccgcttttga
    39181 aaacagcgcc actcgtgctt ttggtcctgc tggttttctc gagcaggatg actcggagaa
    39241 ctggtgtgaa atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct
    39301 ggaaatgggg cttggtcagg aaaagcgccg cgacgacggc attcctggca ttactaacta
    39361 tatcttttca gaaactgccg ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag
    39421 cgaaagctgg caggaagtgc tcgataaaac cgccgcttac cagcaggagg tgatgaaatg
    39481 agtgcgcaag tttcactaga gttacatctc cgcattagcc agtttctctt tcacgaagcc
    39541 agcttactgg acgactggaa atttcgtgac tggctggcgc agctcgacga agagattcgt
    39601 tacaccatgc gcaccacagt taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca
    39661 ccgacaacct ggatttttaa tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg
    39721 gaaacgggca tggcctgggc agaagagccg ccgtcacgca cccgtcactt aatcagcaac
    39781 tgccagataa gcgaaaccga catcccaaac gtatttgctg tgcgggtaaa ttatctgctt
    39841 tatcaggcac aaaaagagcg cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt
    39901 cgccgtctgg aagatgacaa ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg
    39961 gtaatcactt cccataacct gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg
    40021 ttgcggatgt gccggagggt gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt
    40081 tcaacgttgg cggcgagttt tatgccatta acgatcgttg cagccatggt aatgcgtcga
    40141 tgtcagaagg gtatctggaa gatgacgcca cggtggagtg cccgctacac gccgccagtt
    40201 tttgcctgaa aacggggaaa gcgttatgcc tgcccgccac cgatccgctc accacttatc
    40261 cagtacacgt tgaaggtagt gacattttca tcgacttacc ggaggcgcag ccatgagcga
    40321 tctgcataac gagtccattt ttattaccgg cggcggatcg ggattagggc tggcgctggt
    40381 cgagcgattt atcgaagaag gcgcgcaggt tgccacgctg gaactgtcgg cggcaaaagt
    40441 cgccagtctg cgtcagcgat ttggcgaaca tattctggcg gtggaaggta acgtgacctg
    40501 ttatgccgat tatcaacgcg cggtcgatca gatcctgact cgttccggca agctggattg
    40561 ttttatcggc aatgcaggca tctgggatca caatgcctca ctggttaata ctcccgcaga
    40621 gacgctcgaa accggcttcc acgagctgtt taacgtcaac gtactcggtt acctgctggg
    40681 cgcaaaagcc tgcgctccgg cgttaatcgc cagtgaaggc agcatgattt tcacactgtc
    40741 aaatgccgcc tggtatcctg gcggcggtgg cccgctgtac accgccagta aacatgccgc
    40801 aaccggactt attcgccaac tggcttatga actggcaccg aaagtgcggg tgaatggcgt
    40861 cggcccgtgt ggtatggcca gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac
    40921 ctcgataatg cagtctctga cgccggagaa aattgccgcc attttaccgc tgcaattttt
    40981 cccgcaaccg gcggatttta cggggccgta tgtgatgttg gcatcgcggc gcaataatcg
    41041 cgcattaagc ggtgtgatga tcaacgctga tgcgggttta gcgattcgcg gcattcgcca
    41101 cgtagcggct gggctggatc tttaaggaag cacgatgaaa gaaaaaacga tcattattgt
    41161 cggtggcgtg caagcggcgg caatggctgc ggcctcgcta cgccagcaag ggttcaccgg
    41221 tgagctgcat ctgttttccg atgagcgaca tcttccttat gaacgacctc cactctcgaa
    41281 atccatgttg ctggaagatt ccccgcagtt acagcaggtg ttacccgcta actggtggca
    41341 ggaaaacaat gttcatctgc attccggtgt aaccatcaaa acgctgggtc gcgacacacg
    41401 agagttagtg ttaaccaacg gcgaaagctg gcactgggat cagcttttta tagcaaccgg
    41461 cgcggcagct cgaccgctgc cgttgcttga tgcactggga gaacgctgct ttaccctacg
    41521 ccatgccggt gatgccgcca gactgcgaga agttctgcag cccgaacggt cagtcgtgat
    41581 tgtcggtgcc ggaactattg gtctggaact ggctgccagc gccacgcagc gcagcgcagc
    41641 gcagatgtaa ggtgacagtg attgaactgg cggcaaccgt catgggccgt aatgcaccac
    41701 cgcccgtgca acgctatctt ttacagcgcc accagcaggc tggtgtgcgc attctgctca
    41761 ataatgccat tgaacatgta gtcgatggtg aaaaagtaga actgacgctg caaagtgggg
    41821 aaacgcttca ggctgatgtg gtgatttacg gtattggtat cagcgccaac gagcaactgg
    41881 ctcgcgaggc caaccttgat actgccaatg gcattgtcat tgatgaggct tgccgcacct
    41941 gcgatcccgc gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac
    42001 tacaccgctg tgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa
    42061 tgttagggct accgctaccg ctactgccgt ggttctggag cgatcagtac agtgataact
    42121 tacagtttat tggcgatatg cgtggcgat
//
LOCUS       DAJVTY010000011        40505 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVTY010000011 DAJVTY010000000
VERSION     DAJVTY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40505
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.011"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1388)
                     /locus_tag="OQQ66_000591"
     CDS             complement(<1..1388)
                     /locus_tag="OQQ66_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1903631.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFI"
     gene            complement(1385..2092)
                     /locus_tag="OQQ66_000592"
     CDS             complement(1385..2092)
                     /locus_tag="OQQ66_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1903632.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2255..3232
                     /locus_tag="OQQ66_000593"
     CDS             2255..3232
                     /locus_tag="OQQ66_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1903633.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3302..3784)
                     /locus_tag="OQQ66_000594"
     CDS             complement(3302..3784)
                     /locus_tag="OQQ66_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1903634.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4019..6601
                     /gene="leuS"
                     /locus_tag="OQQ66_000595"
     CDS             4019..6601
                     /gene="leuS"
                     /locus_tag="OQQ66_000595"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1903635.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6616..7197
                     /gene="lptE"
                     /locus_tag="OQQ66_000596"
     CDS             6616..7197
                     /gene="lptE"
                     /locus_tag="OQQ66_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1903636.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7197..8228
                     /gene="holA"
                     /locus_tag="OQQ66_000597"
     CDS             7197..8228
                     /gene="holA"
                     /locus_tag="OQQ66_000597"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1903637.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8230..8871
                     /gene="nadD"
                     /locus_tag="OQQ66_000598"
     CDS             8230..8871
                     /gene="nadD"
                     /locus_tag="OQQ66_000598"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1903638.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIANKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8895..9505
                     /locus_tag="OQQ66_000599"
                     /pseudo
     CDS             8895..9505
                     /locus_tag="OQQ66_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            9765..10082
                     /gene="rsfS"
                     /locus_tag="OQQ66_000600"
                     /gene_synonym="rsfA"
     CDS             9765..10082
                     /gene="rsfS"
                     /locus_tag="OQQ66_000600"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1903639.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10086..10553
                     /gene="rlmH"
                     /locus_tag="OQQ66_000601"
     CDS             10086..10553
                     /gene="rlmH"
                     /locus_tag="OQQ66_000601"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1903640.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10584..12485
                     /gene="mrdA"
                     /locus_tag="OQQ66_000602"
     CDS             10584..12485
                     /gene="mrdA"
                     /locus_tag="OQQ66_000602"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1903641.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12488..13600
                     /gene="mrdB"
                     /locus_tag="OQQ66_000603"
                     /gene_synonym="rodA"
     CDS             12488..13600
                     /gene="mrdB"
                     /locus_tag="OQQ66_000603"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1903642.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13611..14699
                     /gene="rlpA"
                     /locus_tag="OQQ66_000604"
     CDS             13611..14699
                     /gene="rlpA"
                     /locus_tag="OQQ66_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1903643.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14839..16050
                     /gene="dacA"
                     /locus_tag="OQQ66_000605"
     CDS             14839..16050
                     /gene="dacA"
                     /locus_tag="OQQ66_000605"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1903644.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16161..16424
                     /locus_tag="OQQ66_000606"
     CDS             16161..16424
                     /locus_tag="OQQ66_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1903645.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16525..17166
                     /gene="lipB"
                     /locus_tag="OQQ66_000607"
     CDS             16525..17166
                     /gene="lipB"
                     /locus_tag="OQQ66_000607"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1903646.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17425..18378
                     /locus_tag="OQQ66_000608"
     CDS             17425..18378
                     /locus_tag="OQQ66_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1903647.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18587..19552
                     /gene="lipA"
                     /locus_tag="OQQ66_000609"
     CDS             18587..19552
                     /gene="lipA"
                     /locus_tag="OQQ66_000609"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1903648.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19653..19856)
                     /gene="tatE"
                     /locus_tag="OQQ66_000610"
     CDS             complement(19653..19856)
                     /gene="tatE"
                     /locus_tag="OQQ66_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1903649.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19985..20773)
                     /locus_tag="OQQ66_000611"
     CDS             complement(19985..20773)
                     /locus_tag="OQQ66_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1903650.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20866..21249
                     /gene="crcB"
                     /locus_tag="OQQ66_000612"
     CDS             20866..21249
                     /gene="crcB"
                     /locus_tag="OQQ66_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1903651.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21304..21513)
                     /gene="cspE"
                     /locus_tag="OQQ66_000613"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21304..21513)
                     /gene="cspE"
                     /locus_tag="OQQ66_000613"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1903652.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21688..22248)
                     /gene="pagP"
                     /locus_tag="OQQ66_000614"
     CDS             complement(21688..22248)
                     /gene="pagP"
                     /locus_tag="OQQ66_000614"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1903653.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            22837..24222
                     /gene="dcuC"
                     /locus_tag="OQQ66_000615"
     CDS             22837..24222
                     /gene="dcuC"
                     /locus_tag="OQQ66_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1903654.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24263..24943)
                     /gene="dpiA"
                     /locus_tag="OQQ66_000616"
                     /gene_synonym="citB"
     CDS             complement(24263..24943)
                     /gene="dpiA"
                     /locus_tag="OQQ66_000616"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS1903655.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(24912..26569)
                     /gene="dpiB"
                     /locus_tag="OQQ66_000617"
                     /pseudo
     CDS             complement(24912..26569)
                     /gene="dpiB"
                     /locus_tag="OQQ66_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            26918..27976
                     /gene="citC"
                     /locus_tag="OQQ66_000618"
     CDS             26918..27976
                     /gene="citC"
                     /locus_tag="OQQ66_000618"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1903656.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            27991..28287
                     /gene="citD"
                     /locus_tag="OQQ66_000619"
     CDS             27991..28287
                     /gene="citD"
                     /locus_tag="OQQ66_000619"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1903657.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28284..29192
                     /gene="citE"
                     /locus_tag="OQQ66_000620"
     CDS             28284..29192
                     /gene="citE"
                     /locus_tag="OQQ66_000620"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1903658.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29203..30735
                     /gene="citF"
                     /locus_tag="OQQ66_000621"
     CDS             29203..30735
                     /gene="citF"
                     /locus_tag="OQQ66_000621"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1903659.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            30739..31289
                     /gene="citX"
                     /locus_tag="OQQ66_000622"
                     /pseudo
     CDS             30739..31289
                     /gene="citX"
                     /locus_tag="OQQ66_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31264..32141
                     /gene="citG"
                     /locus_tag="OQQ66_000623"
                     /pseudo
     CDS             31264..32141
                     /gene="citG"
                     /locus_tag="OQQ66_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32192..33655
                     /gene="citT"
                     /locus_tag="OQQ66_000624"
     CDS             32192..33655
                     /gene="citT"
                     /locus_tag="OQQ66_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS1903660.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            33766..34572
                     /gene="rna"
                     /locus_tag="OQQ66_000625"
     CDS             33766..34572
                     /gene="rna"
                     /locus_tag="OQQ66_000625"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1903661.1"
                     /translation="MKEFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSRGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
     gene            34802..35212
                     /gene="rnk"
                     /locus_tag="OQQ66_000626"
     CDS             34802..35212
                     /gene="rnk"
                     /locus_tag="OQQ66_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1903662.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(35443..36681)
                     /locus_tag="OQQ66_000627"
     CDS             complement(35443..36681)
                     /locus_tag="OQQ66_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS1903663.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            36902..37330
                     /gene="uspG"
                     /locus_tag="OQQ66_000628"
     CDS             36902..37330
                     /gene="uspG"
                     /locus_tag="OQQ66_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1903664.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(37451..39016)
                     /gene="ahpF"
                     /locus_tag="OQQ66_000629"
     CDS             complement(37451..39016)
                     /gene="ahpF"
                     /locus_tag="OQQ66_000629"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1903665.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNLGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(39187..39750)
                     /gene="ahpC"
                     /locus_tag="OQQ66_000630"
     CDS             complement(39187..39750)
                     /gene="ahpC"
                     /locus_tag="OQQ66_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1903666.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            40122..>40505
                     /gene="dsbG"
                     /locus_tag="OQQ66_000631"
     CDS             40122..>40505
                     /gene="dsbG"
                     /locus_tag="OQQ66_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1903667.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPY"
BASE COUNT        10049 a        10369 c         9978 g        10109 t
ORIGIN      
        1 aagataaaaa tgtagaaaat aattttgatg acgcccagac agcccggccc accatcgtca
       61 acgggcattg gctgcggttg cggctccggt tcaacagttt gcttctcggt aaaattcgcc
      121 gcctgaatta ataatcgacg aataattaat gcttccagtg gacaacgatc ttctgcatca
      181 agacggctta cgatcggcat taatgattct tctgtcggtt ggctggcctg cactaattgt
      241 ttggcaacta aatataatgg ccataagaga tgttctgtat cacgcgtgag tgagatccgc
      301 ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac gccccagcgc agagagtttt
      361 tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa
      421 agtagctgtt taccctgatc gttaaccagt tcacgagctt caatcatcat caccactaac
      481 gggtaataat cgacccgatg ctgacggcac cattgcaata ttaaagcatc cgcctgggtg
      541 gagggatact cgcgccagta gtcacaaagt tctgccagca agctttcgcc ttctccacaa
      601 tagacgcgtt gcgcgtattc ataataacgc gcatcttcat tatcaggttc agcttcccgc
      661 cacgaccggg cgtaatccag aagttcgggg atatccaggc gacctgcgct ataccagcgg
      721 agtaaatttc tgtgcagttt tgcatcatcg gggatgatca ctgcgcgatg ttgcgctaaa
      781 tacaatgtca gccattctgg atgcgatgac caaatccgag cgaacatttg gtaaaaatcg
      841 atggtgtcat tttgcacggc aacgggcaga tgcagaatgg tctggaagtt aaaaacattc
      901 cccttcgcaa tggcatataa aaaggattcc agttcttctt cgtctatttc cccgacgttt
      961 tcctcctgca accaccgcaa tctttctgct aataacacca cgcactcgaa ggataaatgg
     1021 gcagtgttca tggcgattgt gcacagcgac cagcgtaatt catcaatctc ttccatcgag
     1081 caataattta attgctgaat gaatcgctgc caggcggagg gcagaaaacg ttcgctatca
     1141 gaggcaagta acgcgcgaaa ggcgagcaga atttcatgtt ctgctacctc atattcttct
     1201 ggttgccaaa cagatttagc aggcgactgc gcaatccgta gcgcctcctc atacgcctga
     1261 cgaagttgtt taaaaccctg cggttcggtt tctggatgaa aggacggtaa cagcgccaga
     1321 taagcgcggc ggataatatc gacgtcagtc gtttcctcta tatcgaggat cttccagcaa
     1381 tttttcataa gtcgctcata tccttaagcg tttccgggca tgtggggtag tcagccagtg
     1441 gttgccagga caaggtaggg taatacgcat catcactggc aaataattga taaaacatat
     1501 cattgccgga atcgtcgtat tctttacgcg ctaagaattc gcgtaataaa ggcagatgat
     1561 catcttcggg agactgccaa tattgccgac caaaggtata accggcaaag tattgccgcc
     1621 agttactata ataacgtaga gcccgtagat gaatacgcga ctggatccat agactttctt
     1681 cttcactcaa ccagttatta agtacgccca ttcgacttaa aaaacccatc cggacataat
     1741 cccatgcttt aattcctccc tcaccacagc attcggctgt gctggcgaca aattgcgcat
     1801 aagcctgacc ttgctcattt aattcagcaa gatagtcacg ccattcacat ggagaataac
     1861 gaaaccagcg gtgataaaac cctgccaacc gagcggcatg accgttctcg atcattcggt
     1921 gaattgtgcg cagcaggtca tcacggtcat caattcccca tgaggaagaa aggtcaaaaa
     1981 agcatgtacc taaatacatt ttgggggatg aatgcgaagg taaacagcat tcattgcgta
     2041 tttccatcgg agtggaaagt gcaaacaata ttttttgcga ccccatgtcc atataattta
     2101 tccttaaaca ttcatttaat gaatgtaaac tacatttcga gactcagtta tttatacttt
     2161 tctgacgcga cataatacgc ataaaataag gcttacgcaa aaatggaaca cataaaatat
     2221 aatatgtttt atctacaacc ataaggaaat gataatgatt tttacgtcaa gttgctgcga
     2281 taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata
     2341 tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg acgaaaaggc
     2401 cttttactgg ctgaagctgg ccgccgagca aggtcattgt gaagcacaat attccttagg
     2461 acaaaagtat actgaggata aaagccgtca taaagataat gagcatgcca tcttttggct
     2521 gaaaaaaact gccctacaag gccatacttt cgcttccaac gcccttggct ggatactgga
     2581 tcgtggagaa gaccccaatt ataaagaagc ggttgtctgg tatcagatag ccgcggagag
     2641 cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca acggagtcgc
     2701 aaaagactat gcgctggcat ttttctggta caaacaagct gcattacaag gccatagtga
     2761 cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc aaaacaagac
     2821 actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcacg cccagtttca
     2881 aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta
     2941 ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat
     3001 gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa
     3061 agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg
     3121 agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc
     3181 cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt agagataaaa
     3241 acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg aaatcagact
     3301 attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg catagcgtca
     3361 gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca
     3421 gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc
     3481 gccattcaag ctgcgtttta tcggtgatat ctgccagctc ctgccataag ctttctttaa
     3541 tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga
     3601 actcttccag gtcacgtctt acagctcgcg tcagctcatc gacctcggtt cgcgttaact
     3661 cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc
     3721 cattgcgcag gcgttcgctc agtgacgcga ccagttcacg gtaatattga gcaaccttgt
     3781 tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg
     3841 ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc
     3901 aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct
     3961 gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat
     4021 gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa
     4081 gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct
     4141 tccctatcct tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt
     4201 gatcgcccgc taccagcgta tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc
     4261 gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac
     4321 gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg
     4381 gagccgcgag ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac
     4441 cgagctgtat aaaaaaggcc tggtatataa gaagacttct gcggtcaact ggtgcccgaa
     4501 cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac
     4561 caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga
     4621 gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa gcatgcagcg
     4681 taactggatc ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa
     4741 cacgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt
     4801 agctgcgggt catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt
     4861 tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa
     4921 aggcgtcgat actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg
     4981 ggcagcaaac ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca
     5041 cgaccagcgc gactacgagt ttgcctctaa atacggcctg aacatcaaac cagttatcct
     5101 ggctgctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct
     5161 gttcaactct ggcgaattca acggtcttga ccatgaagcg gccttcaacg ccatcgccga
     5221 taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg
     5281 tgtttctcgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac
     5341 cgtaatgccg accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga
     5401 cggcattacc agcccgatta aagcagatcc ggagtgggca aaaactaccg ttaacggtat
     5461 gccagcgctg cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc
     5521 gcgctacact tgcccagagt acaaagaagg tatgctggat tccgaagcgg ctaactactg
     5581 gctgccggtg gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt
     5641 ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa
     5701 acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg
     5761 cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat
     5821 cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc
     5881 caagtcgaag aacaacggta tcgacccaca ggtgatggtt gaacgttacg gcgcggacac
     5941 cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctagaat ggcaggaatc
     6001 cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggttt acgagcacac
     6061 agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg actgaagatc agaaagcgct
     6121 gcgtcgcgat gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac
     6181 cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccaac
     6241 cgatggcgag caggatcgcg ctctgatgca ggaagcgctg ctggccgttg tccgtatgct
     6301 taacccgttc accccgcaca tctgcttcac gctgtggcag gaactgaaag gcgaaggcga
     6361 tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct
     6421 ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc accgttccgg tggacgcaac
     6481 ggaagaacag gttcgcgaac gtgctggcca agaacatctg gtagcaaaat atcttgatgg
     6541 cgttaccgtg cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta
     6601 agcgcgggag gaagcgtgcg atatctggca acattgttgt tatctctggc ggtgttaatc
     6661 accgccgggt gtggctggca tctgcgtgat accacgcagg ttccttccac tatgaaggtc
     6721 atgatcctgg actcaggcga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta
     6781 cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg
     6841 cgtttgggta aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca
     6901 gcagagtatc agatgatcat gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc
     6961 tacccgatta gcgccaaagt cttccgttcg ttctttgata acccgcaaat ggcgttagcg
     7021 aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg
     7081 attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg
     7141 accacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga
     7201 ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc
     7261 ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag
     7321 ctgcggcaca aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga
     7381 atgcgatctt ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc
     7441 tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg
     7501 gacttctgca tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag
     7561 aaaatgccgc ctggtttact gcgcttgcga atcgcagcgt gcaggtgacc tgtcagacac
     7621 cggagcaggc tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac
     7681 tggatgacgc ggcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg
     7741 ctcaggcact ggagcgttta tcgctgctct ggccagacgg caaattgaca ttaccgcgcg
     7801 ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt
     7861 tgatgggaaa aagtaagcgc acattgcata ttcttcagca actgcgtctg gaaggcagcg
     7921 aaccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac
     7981 gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc
     8041 gccggggcat gatgggcgag gcgttaaatc gcttaagtca gacgcagtta cgtcaggccg
     8101 tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg
     8161 cagagctgga agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg
     8221 acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat
     8281 ggtcatctaa aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc
     8341 atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa
     8401 cacatgcttg aactggcgat tgccaacaag ccattattta ctcttgatga acgcgagcta
     8461 aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaagga
     8521 ccggacgtgc cgctggcgtt tattattggt caggattcac tgctgacctt tccgacctgg
     8581 tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac
     8641 ccacttgaaa tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac
     8701 caggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt
     8761 aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta
     8821 ttgccggaac cggtactgac ttacattaac caacaaggct tgtatcgctg atacctgtcg
     8881 ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatatcg
     8941 atggtcttta cagcggtcat gcgccccccc cctgaccgcg cgcggtattg agcaagcgca
     9001 aaatctgcat acgctgctac atggtgtttc ctttgatctg gttttatgca gtgaactgga
     9061 acgggcacag cataccgcgc gactggttct cagtgaccgc cagctccccg tgcaaatcat
     9121 ccctgaactc aacgaaatgt tttttggcga ctgggagatg cgacatcatc gcgacctcat
     9181 gcaagaagat gccgaaaact atagcgcgtg gtgcaatgac tggcagcatg caatccccac
     9241 gaacggtgaa ggatttcagg cattttcgca acgtgtggaa cgctttatcg caaggcttag
     9301 tgaatttcag cactatcaga atattttagt cgtcagccat cagggtgtac tgagtctgtt
     9361 aatcgcccgt ttaattggca tgcctgccga ggctatgtgg cattttcgcg ttgaccaggg
     9421 gtgttggagc gccattgata tcaaccaaaa attcgcgacg ctacgcgtcc tcaatagccg
     9481 tgccatcggg gtcgataatg catgactttt ctgttttttt acgggtaagc cgcaacgacc
     9541 attgacagac ccgggcaggc tgatattctc cgcagccaga cttttttcgc cagacacgac
     9601 tttgtagaaa ttgttttaca aaaatggcga tgcaatctgc ggcgcggggt gggatgatag
     9661 cccactttcg aaagccgatt cggcgacaat tgtcccgaaa tcgcctctgg ttcaggtata
     9721 ctgacagacc atttttatct atttgattca cccaggggga aaacttgcag ggtaaagcac
     9781 tccaggattt tgttatcgac aaaattgatg acctcaaagg tcaggacatc atcgccttag
     9841 acgttcaagg caaatccagc atcaccgact gcatgatcat ctgtacgggt acgtccagcc
     9901 gtcatgttat gtccattgct gaccacgttg tgcaggagtc tcgcgcagcg ggcctgttac
     9961 cgctcggcgt agaaggtgaa aacagcgccg actggattgt cgtggatttg ggcgatgtga
    10021 ttgtccatgt catgcaggaa gagagccgtc gcctgtatga actggaaaaa ctctggagtt
    10081 aatgcgtgaa gctgcaactt gtcgccgtgg gaacgaaaat gccggactgg gtacaaaccg
    10141 gttttaccga gtacctgcgt cgttttccga aagatatgcc cttcgagctg attgaaattc
    10201 cggccggaaa acgcggcaag aatgcggaca tcaagcgcat actcgacaaa gagggtgagc
    10261 agatgttggc ggccgcaggc aaaaaccgca ttgtcaccct cgatattcca ggcaagccct
    10321 gggatacgcc gcagttagcc gctgagctgg aacgctggaa gctggatggt cgcgacgtca
    10381 gtctactgat tggcgggcct gaagggttgt cgcctgcctg taaagcggcg gcggagcaga
    10441 gctggtcgct gtcggcgctt accctccccc atccgctggt tcgcgtgctg gtcgcagaga
    10501 gtctgtaccg ggcgtggagc atcaccacca accatcctta tcaccgtgag tgataaggga
    10561 gctttgagta gaaaacgcag cggatgaaac tacagaactc ttttcgcgac tatacggcag
    10621 agtccgcgct gtttgtgcgc cgggcgctgg tcgccttttt ggggattttg ctgctgaccg
    10681 gcgtgcttat cgccaacctg tataatctgc aaattgttcg ctttaccgac taccagaccc
    10741 gctctaatga aaaccgcatt aagctggtgc ctatcgcgcc cagccgcggc attatttacg
    10801 accgtaatgg tatccctctg gccctcaacc gcactatcta ccagatagaa atgatgccag
    10861 agaaagtcga taacgtgcag caaacgctgg acgctttgcg cagcgtggtt gatctgaccg
    10921 atgacgatat tgctgcattc cgaaaagagc gcgcacgttc acaccgtttc acctctattc
    10981 ccgtgaaaac taacctgacc gaagtacaag tagctcgctt tgccgtcaat cagtaccgtt
    11041 ttccgggggt tgaagttaaa ggctataaac gtcgttacta tccttacggt tcggcgttga
    11101 cccacgtcat cggctatgtg tcgaaaatca acgataaaga cgtcgaacgc ctgaataatg
    11161 acggcaaact ggccaactat gcggcaacgc atgatatcgg taagctgggc attgagcgtt
    11221 actatgaaga cgtgctgcac ggtcagaccg gttatgaaga ggttgaagtt aacaaccgtg
    11281 ggcgtgttat ccgccagtta aaagaagtac caccgcaagc cggacacgat atttacctga
    11341 cgctggatct caaactccag caatatattg aaacgctgct ggcgggtagc cgcgcagctg
    11401 tggtagtcac cgatccgcgt acaggtgggg tgctggcgct ggtttccacg cctagttatg
    11461 acccaaactt gtttgttgac ggtatctcca gcaaagatta ttccgccttg ttgaacgacc
    11521 cgaatacacc gctggtgaac cgcgccacac agggggttta tcctcccgcg tctacagtta
    11581 aaccctatgt ggcggtttcg gcattgagcg ccggggtgat cacgcgcaat acgacgctgt
    11641 ttgacccagg ctggtggcaa ctgccaggtt cggaaaaacg ttatcgtgac tggaaaaaat
    11701 ggggccacgg gcgtctgaat gtcacaagat cgctggaaga atctgcggat accttcttct
    11761 atcaggtggc ctacgatatg gggatcgatc gcctctccga atggatgggt aaattcggtt
    11821 atggtcatta caccggtatc gacctggcgg aagaacgttc cggcaacatg cctacccgcg
    11881 aatggaaaca gaaacgcttt aaaaaaccgt ggtatcaggg tgacaccatt ccggttggta
    11941 tcggtcaggg ttactggaca gcgaccccaa tccagatgag taaggcactg atgatcctga
    12001 ttaatgacgg tatcgtgaag gttccgcatt tgctgatgag caccgccgaa gacggcaaac
    12061 aggtgccatg ggtacaaccg catgaaccgc ccgtcggcga tattcattcc ggttactggg
    12121 agctggcgaa agacggtatg tacggtgttg ctaaccgccc taacggtacg acgcataaat
    12181 actttgccag cgcaccgtac aaaattgcgg cgaaatctgg taccgctcag gtcttcggtc
    12241 tgaaagcgaa cgaaacctat aatgcgcaca aaattgccga gcgtttacgt gaccacaaac
    12301 tgatgaccgc ctttgcgcca tacaacaatc cgcaagtggc tgtcgccatg attctggaga
    12361 acggcggcgc gggtccggcg gttggtacgc tgatgcgtca gatcctcgac cacattatgc
    12421 tgggtgataa caacaccgat ctgcctgcgg aaaatccagc ggttgccgca gcggaggacc
    12481 attaatcatg acggataatc cgaataaaaa aacattctgg gataaagtcc atctcgatcc
    12541 cacaatgctg ctgatcttac tggcattgct ggtttacagc gccctagtta tctggagcgc
    12601 cagcggtcag gatattggca tgatggagcg taaaatcggc caaatcgcga tgggtctggt
    12661 catcatggtg gtgatggcgc aaattcctcc acgcgtttat gaaggctggg ccccctatct
    12721 ctatatcatc tgtattattt tgctggtggc ggtagatgct ttcggtgcca tctctaaagg
    12781 tgctcaacgc tggctggacc tcggtattgt tcgttttcag ccgtcggaaa ttgccaaaat
    12841 agccgtacca ctgatggttg cgcgctttat caaccgcgac gtttgcccac cgtcgttgaa
    12901 gaacacaggc atcgcgctgg tgctgatatt tatgcccacg ctgctggtgg ctgcacagcc
    12961 tgacctggga acatcaatcc tcgttgcgct ttccggtctg tttgtactgt tcctctctgg
    13021 ccttagctgg cgtctgattg gcgtcgcagt agtgctggtg gcggcgttca ttcctattct
    13081 gtggttcttc ctgatgcatg attaccagcg ccagcgcgta atgatgctcc tagacccgga
    13141 atcagaccca ctcggcgcgg gctatcacat tattcagtct aaaattgcta ttggctccgg
    13201 cggattacgc ggcaaaggct ggctgcatgg cactcagtca caacttgaat ttctacccga
    13261 acgccatacc gactttatct tcgcggtact ggcggaagag ctgggattag tgggcatttt
    13321 gattctgctc gctctctaca ttctgctgat catgcgcggg ctgtggatag ccgccagagc
    13381 gcaaaccacc tttggtcgcg tcatggctgg cggcttaatg ctgatattat tcgtttatgt
    13441 cttcgtaaat attggtatgg taagcggtat tctgccggtt gtaggggttc cgctcccact
    13501 ggtcagttat ggaggatcag cgctaattgt gctgatggct gggttcggga ttgtaatgtc
    13561 aatccacacc cacaggaaaa tgttgtcgaa aagcgtgtaa gaggtgcgca atgcgtaagc
    13621 agtggctcgg gatctgcatc gcggcaggaa tgctcgcggc atgtacaagc gatgatggtc
    13681 agcaacagac ggtaagtgta ccgcagcctg cggtatgtaa cggccccata gttgaaatta
    13741 gcggggcgga cccgcgtttc gaaccactga acgcgacggc aaatcaggat taccagcgcg
    13801 acggtaaaag ctacaaaatc gtgcaggatc cgtctcgatt tagccaggcg ggactggcgg
    13861 caatctatga tgccgaacca ggcagtaacc tgacggcctc tggcgaagct ttcgatccga
    13921 cacagctgac ggcggcccat ccaacgcttc cgatccccag ctacgccaga atcactaacc
    13981 tggctaacgg gcgaatgatc gtggtgcgca ttaatgatcg cggcccttac ggcaacgacc
    14041 gcgttatttc gctttctcgc gcggcagctg accgtcttaa cacgtcaaac aacaccaaag
    14101 ttcgtatcga tccgattatt gtcgcccagg atggttcgct ttctggtcct ggtatggctt
    14161 gtaccacagt cgccaaacag acttacgccc tgcctgcacc tcccgattta agcggtggcg
    14221 cgggaacaag ttcagtgtct ggcccgcagg gtgacattct tccggtcagt aattcgacgc
    14281 taaaaagcga agatccgacc ggcgcgccgg taaccagtag cggtttcctc ggcgcaccaa
    14341 cgaccttagc gcctggtgta ctggaaggca gcgaaccgac gcctgcgcca cagcccgttg
    14401 ttacagctcc gtcgacaacg cctgcaacct cgcctgcaat ggtgacaccg caagccgcct
    14461 cgcaaagcgc cagcggcaac tttatggtgc aagtcggggc cgttagcgat caggctcgtg
    14521 cgcaacagta ccaacagcaa ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa
    14581 atggcgcggt ctggcggatc cagcttggcc ccttcgccag caaagccgaa gccagtacct
    14641 tgcagcaacg tttgcaaacc gaagcccaat tacagtcatt tattaccacc gcgcagtagc
    14701 gtaaagcagg catctgatgt gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa
    14761 gtcagatgcc tgccggtagt gcatttgcta tagtagggca ctttttttaa ttccatcacg
    14821 gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa gcgcctggcg
    14881 ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga cctgaatatc
    14941 aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct gattgactat
    15001 aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg
    15061 accaaaatga tgaccagtta cgttatcggc caagcaatga aagccggtaa atttaaagaa
    15121 actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt gtttaaaggt
    15181 tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct gatccgcggt
    15241 attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg ctgattttgc cgctggtagc
    15301 caggacgctt ttgttggctt gatgaacagc tacgttaacg ccctgggcct gaaaaacacc
    15361 cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc gcgcgatatg
    15421 gcgctgatcg gccaggcgtt gatccgtgac gtaccgaatg aatactcgat ctataaagaa
    15481 aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt atgggataac
    15541 agcctgaatg tcgacggcat caaaaccgga cacactgaca aagcaggtta caaccttgtt
    15601 gcttctgcga ctgaaggcca gatgcgcttg atctctgcgg tgatgggcgg acgtactttt
    15661 aaaggccgtg aagccgaaag taaaaaactg ctgacctggg gcttccgttt cttcgaaacc
    15721 gttaacccac tgaaagtagg taaagagttc gcctctgaac cggtttggtt tggtgattct
    15781 gatcgcgctt cgttaggggt tgataaagac atgtacctga ccattccgcg tggtcgcatg
    15841 aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag
    15901 aatcaggtcg tcggaactat caacttccag cttgatggca aaacgatcga gcaacgcccg
    15961 ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat tgattacatt
    16021 aaattaatgt tccatcactg gtttggttaa aaattaaaca cttgaaagtg taatttccgt
    16081 ccccatatac taagcatcag taaaaaaact cccgccttct ggcgggagtt gctatttaat
    16141 tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa ttccctactc
    16201 cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag gtggttgaag
    16261 tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc agcaaaggca
    16321 actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt gaaatactgt
    16381 atgaagaact gggcaaaatc gatattgtcc gcatggttct gtaactcgct tctccgttac
    16441 ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat atacttttcc cccactttta
    16501 ctcattctcc acggagatgc cgttttgtat caggataaaa ttcttgtccg ccagctcggt
    16561 cttcagcctt acgagccaat ctcccaggct atgcatgaat tcaccgatac ccgcgatgat
    16621 agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca aggtcaggca
    16681 ggaaaagcgg agcacatttt aatgccgggt gatattccgg tgatccagag cgatcgcggt
    16741 gggcaggtga cttatcacgg gccgggacaa caggtgatgt atgtgttgct taacctgaaa
    16801 cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt ggtgaatacc
    16861 ctggctgaac tgggtataga agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg
    16921 gaaaagaaaa tttgctcact gggtttacga attcgacgcg gttgttcatt ccacggtctg
    16981 gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg tgggtatgcc
    17041 ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa taatattgct
    17101 ccacgtttac tggaaaatat tttagcgcta ctaaacaatc cggacttcga atatattacc
    17161 gcttaattcc atacatcaat ggcccaattt acactgggtc attactcata atacctgcta
    17221 atgctttaca tctccttcgc gtttgtatat tttctttatc acttgcagaa atgcggtgcc
    17281 acgccttgct ccgtaactca ccccattgat taacctattg aaatagttga ttatggtgag
    17341 caaaagcgtt ccgttccaat tagacataag attattgtaa ttttttaaat attatttttc
    17401 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaacc ctgtttaagc
    17461 agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgctcttaac
    17521 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
    17581 cttaatctgc ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
    17641 gacccattgt ttattcgcaa aggccaaggt gtcactccta ccgcatttgc gatgcatcta
    17701 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
    17761 tatgataagc aacgaacgat aactattgcg accactccct cggtcggagc cctggtcctt
    17821 cctgtcatct atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
    17881 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    17941 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    18001 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    18061 gcgcatgtac ttctgttacc ggaagagcaa aatttcagcg gtctgcggca gagagttcaa
    18121 gaaatgtttc cggaccggca aatcaatttc accagctaca acattttgac aatcgctgca
    18181 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagc
    18241 cgctgctggc cgctggaaaa actgcctttt ccgtccttaa ataaggagca aatagatttc
    18301 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt aatagatgtt
    18361 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
    18421 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
    18481 ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa caatctattg
    18541 aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga gtaaacccat
    18601 tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta tcccggttaa
    18661 aaacgtggca atagagcgcg aagccctgct gcgcaagccg gaatggatga aaatcaagct
    18721 tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa atggcctgca
    18781 ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc acggcacagc
    18841 aacgtttatg atcctcggcg ctatttgtac ccgccgttgt ccgttctgtg atgttgccca
    18901 cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga ccattgccga
    18961 tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg
    19021 tgcccagcac tttgcggatt gcattactgc cattcgggaa aaaagcccgc aaatcaaaat
    19081 tgaaactctg gtgccggatt tccgtggtcg tatggatcgt gctctggata ttctgactgc
    19141 aacgccacca gatgtgttca accataacct ggaaaacgta ccgcgtattt accgtcaggt
    19201 acggcctggg gcagattaca actggtcgct gaagctgctg gaacgcttta aagaagcgca
    19261 tccggaaatc ccgaccaagt ctggtctaat ggtgggactg ggtgaaacca atgaagaaat
    19321 tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc tggggcaata
    19381 tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg atgagttcga
    19441 cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg gtccgtttgt
    19501 ccgttcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt aatcctgtaa
    19561 taattactta catctgtctg ataaaaaacc cgctttttga agcggttttt ttgtatcgaa
    19621 caagatattg agggagcgtc ctgctcgcca cgtcactctt tatgagagag cttttcagcc
    19681 tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcat catcattcat cgccttcttg
    19741 aaccctttaa tggccgctcc aaggtctccg cccagcgtac gtaacttctt agtcccaaac
    19801 agcagaacga ccagcgccgc aactactagc agtttggtaa tactaatctc acccatagat
    19861 accttcttga catataaccg gcaacagaac gcgccttaaa aaccagagaa tattaacggt
    19921 cattcgacgc gcgcacacaa tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa
    19981 aacatcataa taattgcggc ggcgcaaagc gacggttgtt taagacgggc agttgcgcgc
    20041 gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc atttctgacg
    20101 ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgcccgata tttttgttcc
    20161 cgcactcgcc cgccgccacc atataacagg tggtatccag cgcacgagcg gcaagcaacg
    20221 ttgaccagtg atgctctttg agcggcccgc gaacccaggc ggcaggaagt accaggattt
    20281 cagctccctg taatgcctgt gccagcgcca gctctggaaa gcgtaagtca taacaggtca
    20341 tcagaccgac cttcatccct tccacctcca gtaacggagc gatttcatta ccagcatcaa
    20401 cacggcgtga ttcctgaatg gcaaatgcat catagagatg cagtttggca taacgggcga
    20461 cgatgtttcc tgcctgaagt gccaccagca tattccatgc gcgccccggc gttgaaggaa
    20521 catgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt aaacgtccga
    20581 ggaattcgcc ttccagcagc tgtgctgatt taaccgatag atctgcatca tgatcatcgc
    20641 gtgccagcaa tgcttccggc aggacaaaca gcgatacgtc gttttccgcc gcctgcgcca
    20701 tcaacgaggc acaaatctca gcgttctttt cccacacaga tgtaacagca aactgtcctg
    20761 cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg
    20821 gcgttacact tatcactcat acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt
    20881 ttagcagttt ttattggcgg tggtacggga agcgtggcga gatggctgtt aagtatgcga
    20941 tttaacccac tgcatcaggc gattccgttg gggacgctga cagcaaacct gattggggca
    21001 ttcatcatag gaatgggatt cgcatggttc agcaggatga cgaacattga tccagtgtgg
    21061 aaagtattaa tcaccaccgg attttgtggc ggtctaacaa ccttctcaac attttcggca
    21121 gaagtggtgt ttttgttaca agagggtcgc tttggctggg cattactgaa cgttttcgtc
    21181 aaccttctgg ggtcttttgc catgacagca ctggctttct ggctgttttc ggcctcaacc
    21241 gcacactaaa gacccaaaaa aaacccgctg attaagcggg ttttgaattc ttgctgacgt
    21301 atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag tgatttcgaa
    21361 ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg cagagaagtg
    21421 tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt
    21481 aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat gaaaaataga
    21541 cacaaatgct gcgtcggtta ccagtacacc aattgtggta cgctttgtcc agtgcaactt
    21601 tactaaaaag tgataaaagt cgcgttattt ttgatgttac ttattttagc tattgatttt
    21661 aaagaagtta ctaaaacttc atttgtctca aaactgaaag cgcatccagg caaagtacac
    21721 attgccattg ttgtaggtac ccggaatgta ggtcatctga aaagtcactg ggccataacc
    21781 cacggaggcc aatggcagta gaaccgggag agggatgtaa ttccagttat cgcgtgccgt
    21841 tacgccagcg gtgaatccca gacctaaatg aaaattttca tccgccagcg gtcgccaggt
    21901 actttcccat ccgtatccgg caatcggttc ccatttgttc cacgagtcct taaatgccat
    21961 ggcatacagg ccatgccagt ttcctttttc atcccaacgc gacaggccaa aaccgccacc
    22021 ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca
    22081 ggtgatggca ggaatatata aatcataatg ttcaggctgt tgccaggttg gtgcaatatt
    22141 ttctctaaac gttgtcatcc actcatctgc gttagcaaaa actttaccaa cgctgattaa
    22201 ctgaataaaa acaaaggaaa agatagcgac atatttactc acgttcattt gtgaccataa
    22261 aacatttatc aaaaatctac tactagcata gcaaagctac aattaacata accttaatag
    22321 acccaacata aagaataatc tgaatagctg ctctatttat ttccgcttgt tcaagttaaa
    22381 tatttccgcg catcgattaa agatgatgcg tcgttttctg cgatgggaat agtcaaaaaa
    22441 gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg aaaaattatt ttatttttta
    22501 acgagataca acaatcacct taacaaagta tataatatat atcgtcaacc tatgctcata
    22561 ttagattatt ttttgagcgc acagccgcag ctaacaccat tgcaattaac aaatttgcat
    22621 caatccacca tcaatttgca cacattatta tgtgataatt gccaaccgct aaatatgcgt
    22681 tttgttatct atgtataaaa acagtaactt caatgtctta atggcagttt ttcttgattt
    22741 taatcagcat tcatcgccaa tttattgggc atattttttc cttaagcttt aggaattttt
    22801 tattatttac tttggggcct ggagacagga aaaattatgc tgacattcat tgaactcctt
    22861 attggggttg tggttattgt gggtgtagct cgctacatca ttaaagggta ttccgccact
    22921 ggtgtgttat ttgtcggtgg cctgttattg ctgattatca gtgccattat ggggcacaaa
    22981 gtgttaccgt ccagccaggc ttcaacaggc tacagcgcca cggatatcgt tgaatacgtt
    23041 aaaatattac taatgagccg cggcggcgac ctcggcatga tgattatgat gctgtgtgga
    23101 tttgccgctt acatgaccca tatcggcgcg aatgatatgg tggtcaagct ggcgtcaaaa
    23161 ccattgcagt atattaactc cccttacctg ctgatgattg ccgcctattt tgtcgcctgt
    23221 ctgatgtctc tggccgtctc ttccgcaacc ggtctgggtg ttttgctgat ggcaacccta
    23281 tttccggtga tggtaaacgt tggtatcagt cgtggcgcag ctgctgccat ttgtgcctcc
    23341 ccggcggcga ttattctcgc accgacttca ggggatgtgg tgctggcggc gcaagcttcc
    23401 gaaatgtcgc tgattgactt cgccttcaaa acgacgctgc ctatctcaat tgctgcaatt
    23461 atcggcatgg cgatcgccca cttcttctgg caacgttatc tggataaaaa agagcacatc
    23521 tctcatgaaa tgttagatgt cagtgaaatc accaccactg ctcctgcgtt ttatgccatt
    23581 ttgccgttca cgccgatcat cggtgtactg atttttgacg gtaaatgggg tccgcaatta
    23641 cacatcatca ctattctggt gatttgtatg ctgattgcct ccattctgga gttcctccgc
    23701 agctttaata cccagaaagt tttctctggt ctggaagtgg cttatcgcgg gatggcagat
    23761 gcgtttgcta acgtggtgat gctgctggtt gccgctgggg tattcgctca ggggcttagc
    23821 accatcggct ttattcaaag tctgatttct atcgctacct cgtttggttc ggcgagtatc
    23881 atcctgatgc tggtattggt gattctgaca atgctggcgg cagtcacgac cggttcaggc
    23941 aatgcgccgt tttatgcgtt tgttgagatg atcccgaaac tagcgcactc ttccggcatt
    24001 aacccggcgt atttgactat cccgatgctg caggcgtcaa accttggccg taccctttcg
    24061 cccgtttctg gcgtagtcgt tgcggttgcc gggatggcga agatctcgcc gtttgaagtc
    24121 gtaaaacgca cctcggtacc ggtgcttgtt ggtttggtga ttgttatcgt tgctacagag
    24181 ctgatggtgc caggaacagc agcagcggtc acaggcaagt aaatagtaat gccggaggca
    24241 agtttcctcc ggctttatca tgtcacccac tgtggtatat gcgttgtggt ctgccaactt
    24301 tgccgtgaac aatttcagca ataatcagat ggcggctggc gcaatattca agataacgtc
    24361 tggcagacgt gcggctgatg gtcagtgcct gcgccaccgt ttctgccgta tgttgcacac
    24421 caggctcttt aaacagtttt cgcaccgcgt ttagcgtcaa gggatcaatg ccggtcggca
    24481 gttcgtcttt aggttcaccg cgagcatagg cattaaacat ctcatcaatt tgcttctggc
    24541 tggcgctatc aatactttcc agcatatgtt tacgctggcg gaaacgggtt agcgtttgcc
    24601 ccagccgttc ataggcaatg ggcttaatga gataatcaaa tacaccacaa cgtacggctt
    24661 cagacaccgt ttccatatcg ctggctgcag tggtaaacac cacgtcgccg ggataatgcg
    24721 cctgcaccag ttcatgcaat aaattaatcc ctctgccgtc aggaagatag ttatcgagca
    24781 agattagccc cggcttaaaa cgctcgatca tcattcgggc ctgcgccagg tttcccgcca
    24841 gtaatatctg actgaatccg ggaatgtgac gaatatattc cgcatgcatc tctgccagcg
    24901 gcgtttcgtc ctcaacgatc aatagggtta atggagctgt cattaggttt cactttcgga
    24961 atatagattg aaaataaggt accgcaggga tcattatctt cgagagtgat aacaccaccg
    25021 cagcgcgtta cgtagctggc aatcaagtac aacccaatgc catgttcacc gggctcgtca
    25081 gcacgcgtac tgaccccctg ctcaaatatt ttgtctcgta gagactctgg aacgccgcag
    25141 ccctgatcgg cgacttcaat caccacatca tcgccttcat cgctgaggaa taattcaacg
    25201 atcttgtttc cttcatcgct acgcaggctg gcttcgaagg cgttatcaag taaattgccc
    25261 acaatggctg caaactcggt gctatccagt cctggcggca gttgcgaaag ctggctaccg
    25321 gggacaatga tcatttttag ccccagttcc cgggcgcgct gcactttacc aaaaagcagc
    25381 cccgccacct agcgatcggc aaacgcctcg cgcaggctgt caataagctg ttgctgggcc
    25441 tgagactccc cctgcaccat cgccagcacg cgatcatact ctttcatctg caacagacca
    25501 ttgagcgtcg acatccaatt gagatgctcg tgtcgcaatg tacgaaggct ctcaacgtat
    25561 tgttttattt gcgtcagttg cgcattgagg gtggaaattt cgtctttact gcgaaagctg
    25621 ataatggccc ccagcaaatc atcacctgaa cgaatagctt cccgattggc aataacgctc
    25681 agaccgttaa agttagccac catatcctga cgtttttcat caatctgttc ggtaaagaaa
    25741 tcggcgggcc tgaccacttc agcaatgggt ttacccaacc attgccgtcc gggggaactc
    25801 agccccagca tctttcttgc gttacgattg atggcggtaa tgtaaccgtg cggatccacc
    25861 gcaatcagcc cttcataaac cgaactaaac agcgcctctt gctgacggac cacgcgtgcg
    25921 atttgctttg gctccatgcc catcatctgc cgacggatat gcgcggccag gaaccacgac
    25981 agcaacatca gaatccctaa cagcacgaca aacacacctg ccatcggtaa taaaaactca
    26041 gcccgccagc tatcgatttt actcaccagg tagccaatcg acaccacgcc gatgactttt
    26101 ccatcgtcat caaagattgg cgttttggcg cgcatcgcca tccccattga ccctttcccg
    26161 gtaatgaagt agctctcccc tttctccagc gcgccctgtt tggtgaactg cataggataa
    26221 ccaattttct ccggattagg atggtaaagg cggatcgagt gccggtcgcc atcaccacat
    26281 aatcaaaatc ggtatctctt tgtaatttgt tagcgatggt cgccagccgt ttgtagtcac
    26341 gcgttttcac cgcagagatg acactgtcat tggaggcaat aattttcgcc tgattcattg
    26401 ccatgtcgcg tacatgaagc gttaaatagt cctcaaaact ggccgtaaaa tattgcgcca
    26461 aggctgcaat gacaaatatt gagaacacca gaatcagcaa aaagatacgc agcggaaatg
    26521 ccagtctttg gaaaaatgca aactgtttat tctcgttaag ctgcaacatt gttttccttg
    26581 cgggttatcc cgagcctctt tgacagatgt aattaaaatg ttgaatcaaa atacatagat
    26641 attaattatg atcgtggcaa attttattca ttaaatcaat taaatcactt aaacctgcag
    26701 gtcatttcgt aattacatta attaaaaaac ttaaaccatt aaataaataa aaatttcacg
    26761 ccgaaatact ccttaggatg tatagcgaaa ggagaaaaag atatacctcg atcacccccc
    26821 tttctcccaa gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa
    26881 atcgataaca acatgcacct tcaggatact atttattatg ttcggcaatg atattttcac
    26941 ccgcgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa
    27001 tgatttgagc gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct
    27061 tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc gttgctatca gtgaatccgt
    27121 tcgcggtgaa ggactggcgc tgacattagc cactgaactg ataaacctcg cctatgagcg
    27181 gcacagcacg catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg
    27241 cggtttttcc acgctgacca gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac
    27301 gcgactgaaa cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg
    27361 ctgcattgtg atgaacgcca atccctttac gaatggtcac cgttatctga ttcaacaagc
    27421 tgcggcacag tgcgactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc
    27481 ctatgaagac cggctggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt
    27541 gcatcgcggc tccgaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga
    27601 acagagcgtc attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct
    27661 cgctcccgca ctgggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac
    27721 cgcccagtac aaccaggata tgcgctactg gctgaaaacg ccgactatct ccgcaccgcc
    27781 catcgaactg gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg
    27841 ggtacgtcaa ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt
    27901 cacgctgcat tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca
    27961 aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc
    28021 acccttgagt ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac
    28081 ctgcaaatca acagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg
    28141 gacgttctcg cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcaca
    28201 ctggactgca ttttacgtgc acgactggaa gccctgctgg cacgcgccag cggtatcccg
    28261 actctgccat gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca
    28321 cccgccgcag catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca
    28381 tctacccggc tgatgccctg atgtttgacc tcgaagactc cgtggcatta cgtgaaaaag
    28441 acaccgcccg ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa
    28501 ccattgtgcg tgtcaacgcg ctggattccg aatggggtgt taacgacctg gaagccgtcg
    28561 ttcgcggtgg tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg
    28621 atattgaaaa agagatcctg cgtatcgaaa aagcctgtgg tcgtgaaccc ggcagcaccg
    28681 gcctgctggc ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg
    28741 cttccgagcg tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta
    28801 cagaacgctc cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg
    28861 cgcgctctgc gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg
    28921 gattcctgca agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca
    28981 acccgcgtca gattgatctg ctgcacaacc tctacgcacc gacccagaaa gaagtggatc
    29041 acgcccgccg cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt
    29101 ccctgaacgg caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc
    29161 gtgcagaact ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa
    29221 caatctcaac gacaagaacg ggtagcggcc tggaatcgtc gcgctgaatg cgatcttgcc
    29281 gctttccaga actcaccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg
    29341 tgtgccaacc tggaagaagc gattcatcgc tctggtttac aggacggcat gacggtttcc
    29401 ttccatcacg ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg
    29461 aagatgggct ttaaaaacct gactctggcc tccagctccc tgagtgattg ccatgcgccg
    29521 ctggtagaac acattcgcca gggcgtggtt acccgcattt atacctctgg cctgcgcggt
    29581 ccactggcgg aagaaatctc ccgtggtctg ctggcggaac cggtgcaaat ccactctcac
    29641 ggcggacgtg tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc
    29701 gtcccatcct gtgatgaatt cggtaatgcc aacggttaca ccggtaaagc ctgctgtggc
    29761 tccctcggct atgcaatggt tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa
    29821 gaactgctgc cttatccgca taatccggca agcattgagc aagagcaggt tgatttgatc
    29881 gtcaaagttg atcgcgttgg cgatgctgca aaaatcggcg ctggtgcgac ccgtatgacc
    29941 actaacccgc gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactctggc
    30001 tacttcaaag aaggtttctc catgcaaacc ggcaccggcg gcgcttcgct ggcggtaacc
    30061 cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt
    30121 attactgcaa cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgtg
    30181 cagagctttg acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc
    30241 agcgccaacc agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg
    30301 gtggtactga gcgcgctgga aattgacacc cagttcaacg ttaacgtgct gaccggctct
    30361 gacggcgtac tgcgtggtgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt
    30421 tccatcatcg tcgcgccgct ggtacgcggt cgtattccga ctctggtgga taacgtgctg
    30481 acctgcatca ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt
    30541 aacccggcac gtccggagct ggcagaacgt ctgcaggaag cgggcattaa agtggtttcc
    30601 attgagtggc tgcgtgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc
    30661 accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtacat
    30721 caggtgaagg aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca
    30781 attcccgagc tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgt ctggctcaag
    30841 cgccatcctg ttccactggt ctcctttacc gtggttgcgc ctaggccgat taaagacagt
    30901 gaggtcacac gccgaatttt taatcatggc gtgacagcct tgcgtgcctt agccgcaaaa
    30961 cagggctggc aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggtatg
    31021 ttgagcattg ccgcccctgc tcgcgacctc aagctcgcca ccattgagct tgaacatagt
    31081 catcctctcg ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc
    31141 cgccgcgact attcactgcc gcctcgccgc tgcctgttgt gcgagcaaag cgcagccgtc
    31201 tgcgcgcgtg gaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga
    31261 acgatgtcga tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat
    31321 gagtacgccc tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca
    31381 ggtctcgtgg atcgcattaa ctgcggtgcg cataaagata tggcgctgga agatttccac
    31441 cgcagcgcgc tggcgattca gggctggcta ccccgtttca ttgaatttgg tgcctgtagt
    31501 gcggaaatgg caccagaagc ggtactccac ggattacgcc caattggtat ggcttgcaaa
    31561 ggtgatatgt tccgcgccac cgcgggcgta aacacgcata agggcagcat tttttcttta
    31621 gggctactgt gtgcggcaat tggccgtttg cttcagctca accagtcgct aacgccaaca
    31681 accgtttgtt ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc
    31741 aataattcac aactgacggc aggtcaacgg ttgtaccagc agcttgccct taccggcgca
    31801 cgcggtgaag ccgaagcggg ttatccactg gtgatcaacc acgccttgcc gcattacctc
    31861 actctgctgg atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg
    31921 atggcgatca acggcgatac caacgttgct tcgcgcggtg gcgagggggc ctgcgctggc
    31981 tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc gccgatctcg
    32041 attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg
    32101 ccgaccagct gatccttacc tggtttttag cacagattta attatttaag cacttgataa
    32161 atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa
    32221 actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccaacggtat
    32281 gccaccacag gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct
    32341 cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag
    32401 taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca
    32461 ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc
    32521 atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct
    32581 ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat
    32641 tctgctggcg ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt
    32701 cattaaaaac ctgccgccgt tgtttaaatc attccccaac gatccgtccg cgcgtcgtat
    32761 tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt
    32821 caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca
    32881 gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat
    32941 tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc
    33001 aacctgggcg ggtgatgaac tgaaaaccat gggtgcgctg acacgcagag agtggacact
    33061 gattggcctt gtattgctca gcttaggttt gtgggttttt ggcagtgaag tcattaatgc
    33121 tactgcagtt ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg tgccttggaa
    33181 agatattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt
    33241 gatggctaac ggcctgactc gttctggctt tattgactgg ttcgccggta ccatgagtac
    33301 gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt
    33361 tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct
    33421 ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct
    33481 gtctatcggt atcatgggct gtctgacgcc ctatgcaacc ggtcctgggg tgattattta
    33541 cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat
    33601 ttatatctct atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt
    33661 tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc gggcgtgatg
    33721 atttaataga tagtattcag tgtatgtatg agttccacac ccattatgaa agaattctgg
    33781 cgtaacgccg cgttgctcgc ggtttctctg cttcccttct cttctgccaa cgccgtagcg
    33841 ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc tggccctctc ctggcaaacc
    33901 ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg tcgcctgcaa
    33961 accgaaacga ccaacaaagc tgattttctg accgtacatg gtctgtggcc aggattgcct
    34021 aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg ttgcgctact
    34081 cgcccaatcc cgaatctacc agaagcgcac gccagccgga tgtgttcatc gccggaaacc
    34141 gggttatcac tggaaacggc cgctaaacta agtgaagtca tgccaggagc tggcggacgt
    34201 tcctgcctgg aacgctacga atatgccaaa cacggtgcct gctttggttt tgatccggac
    34261 gcatacttcg gtacgatggt acgcctgaat caagaaatta aagagagcga agccggaaaa
    34321 ttccttgcgg ataattacgg taaaacagtg agccgccgtg actttgacgc cgcctttgcc
    34381 aaaagcaggg gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg taaccctgcg
    34441 tatttgactg aaattcagat ctcgatcaaa gctgacgcca tcaacgctcc gctttctgca
    34501 aactcattct tgccacaacc acacccaagc aactgtggca aaacctttgt gattgataaa
    34561 gtgggttatt aatcatctga tttacaaaac acgctaattc gtcttccata aaccgtgacg
    34621 attaagaatt atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact
    34681 tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg
    34741 agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa
    34801 aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct
    34861 ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg
    34921 cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt
    34981 taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa
    35041 aatgaccgat agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg
    35101 gctgcgcgtt ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga
    35161 agtgctggaa ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct
    35221 gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca
    35281 gcaatttttc ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg
    35341 ggaataacca tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt
    35401 atcgtcatgg ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg
    35461 catcgcattc accagacctg aaactgcctt ctgcgcctcc tctgcgcttt gtgcaccggg
    35521 taccagaatc accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc
    35581 ggcctcttca aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc
    35641 aattaacggt aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt
    35701 caacccttta tcaaaggcgt caccaaacag gaaaccgtga ataaatccag cgtagacgcc
    35761 cggtacgcta acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc
    35821 gctgctgccc tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc
    35881 aaaaccgacg gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat
    35941 tgactgtgcc ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc
    36001 ggcgaaactc aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa
    36061 ccgtgcacag gcgattgtca acaatcccac tggaccggca ccatagactg caacgcttga
    36121 accttgttgg atctgcgcat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag
    36181 gaaaagggct ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt
    36241 agggacgcgg acatattccg cctgtccacc aggaacgcca ccatacaggt gactataacc
    36301 aaacaatgcc gcgggagctg gtatctgttt tttattgagc gcagcgcctt tacccgcatt
    36361 ggtattttcg caggcggcat attgctgcat tcgacagaaa aaacagtcgc cacaagcaat
    36421 gacaaacgga attaccaccc ggtcgccttt ttgcaaattt tttacgtcct ttccggtttc
    36481 aacgacttcc cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt
    36541 gcctcgataa agatggagat cagagccaca gatcgccgtt gccgtaatac gcagaataat
    36601 atcatctgcc tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc
    36661 gtgataagtc aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta
    36721 agggtagcta tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctggtg
    36781 ttcagtgaga atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat
    36841 cagggataaa tattataatc atggcgaatg atttgttcat gattaacagg gagaaagggt
    36901 tatgtataag acaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc
    36961 tgttcgccac gctgaattcc tcacccagga tgacggagtt attcatctac ttcacgtact
    37021 acccgggtca gccagcctga gcctggaccg ttttgccgct gatgtgcgtc gttttgaaga
    37081 gcatctgcaa catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga
    37141 tccttcccgc attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt
    37201 ggcagaagaa ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac
    37261 ccatctgtta ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt
    37321 cgttcgctaa cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt
    37381 ttaatgccat aaaaaagccg ctaggtttga ccccggcggc taagcaattg caggtgaatc
    37441 ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt
    37501 tggcaccttc gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg
    37561 ctgcgaatac gcctttgacg ttggtttcgc atttcgcatc aatgataatc tcacccatgc
    37621 ggttacgttc gactgcgcct tcgagccagt tggtgttcgg cagcagacca atctgcacga
    37681 aaataccggc cagttcgatg ttgtgaatat cgccgctgac acgatctcga tattccagac
    37741 caacgacttt gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga
    37801 cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa
    37861 attccagcag cgttacgtgc tcaacgatac ccgccaggtc aattgccgct tccacgccgg
    37921 agttaccgcc gccgataacc gctacgcgtt tacctttaaa cagcgggccg tcgcagtgcg
    37981 ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc
    38041 attttgcacc ggtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt
    38101 caatctgatg cagaccacct tcaactgctg ctgggatcag tttgctggcg ctctggctgt
    38161 cgatcacatc aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc agcttctgcc
    38221 cttcagtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac
    38281 caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg
    38341 ctgcacccgc cgggccggaa ccgacgatta atacgtcata agcatcacgc ttgttcagct
    38401 cttctgccgc acgtttttcc gcgcccgtat caattttggc aacgatttca gtcaacgtca
    38461 tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc
    38521 gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt
    38581 tcagtacgct catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg
    38641 agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact
    38701 gcgcttcttt cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact
    38761 cgtggcccag cggggagcct gcaaaacgtg gcccctggtt ggaacctagg ttggtgatca
    38821 ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca
    38881 gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg
    38941 tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca
    39001 tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctcta
    39061 cggctacctg gatgcagctt gcatcatagt gcggggaaga aaatcgggtg ccgcagcacc
    39121 cgaagaatta ggtgaatttc cgaagtattt cgcgccggag ttggtagcgc gaaagacgaa
    39181 ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt caccttcttt
    39241 ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgtgctg ctttgatttt
    39301 acgcagcagg tcagacgcgt cacggccaat gccttcagcg gtaacttcga ttgcctggat
    39361 gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat cttcacgcat
    39421 gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat atttgatttt
    39481 agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat cggtagatac
    39541 tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa cgtcacccag
    39601 ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc tccagcggcc
    39661 ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct ggtttttaaa
    39721 aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt cgatgagatg
    39781 taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat cgataagttt
    39841 taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg cgataaaaag
    39901 taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt atcttcagcg
    39961 cctgcctgca ggctgaggcg ttgcccttac aaatgcaaca acgacatgga ttacaacacc
    40021 ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg ataggttatg
    40081 ccttttactc gacttttgca ctgactgaaa aggacaaatt aatgttaaaa aagatacttt
    40141 tactggctct gcttcctgca atcgccttcg cagaggaact tcctgctcca gtaaaagcga
    40201 ttgaaaaaca gggcattaca atcatcaaaa cgttcgatgc ccccggagga atgaaaggtt
    40261 atctcggaaa gtatcaggat atgggcgtca ccatctacct gactccagat ggtaagcacg
    40321 ctatctctgg ttacatgtac aacgagaaag gtgaaaacct gagtaacaca cttatcgaaa
    40381 aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa tcccactggc
    40441 tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt cttcgccgat ccgttctgcc
    40501 catat
//
LOCUS       DAJVTY010000012        40293 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVTY010000012 DAJVTY010000000
VERSION     DAJVTY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40293
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.012"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..2298)
                     /locus_tag="OQQ66_000632"
     CDS             complement(<1..2298)
                     /locus_tag="OQQ66_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1903668.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALA"
     gene            complement(2395..2871)
                     /gene="greB"
                     /locus_tag="OQQ66_000633"
     CDS             complement(2395..2871)
                     /gene="greB"
                     /locus_tag="OQQ66_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1903669.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3099..3818
                     /gene="ompR"
                     /locus_tag="OQQ66_000634"
     CDS             3099..3818
                     /gene="ompR"
                     /locus_tag="OQQ66_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1903670.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3815..5167
                     /gene="envZ"
                     /locus_tag="OQQ66_000635"
     CDS             3815..5167
                     /gene="envZ"
                     /locus_tag="OQQ66_000635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1903671.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5243..6865)
                     /gene="pckA"
                     /locus_tag="OQQ66_000636"
     CDS             complement(5243..6865)
                     /gene="pckA"
                     /locus_tag="OQQ66_000636"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1903672.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7244..8968
                     /locus_tag="OQQ66_000637"
     CDS             7244..8968
                     /locus_tag="OQQ66_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1903673.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKIALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9103..9981)
                     /gene="hslO"
                     /locus_tag="OQQ66_000638"
     CDS             complement(9103..9981)
                     /gene="hslO"
                     /locus_tag="OQQ66_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1903674.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10006..10407)
                     /gene="hslR"
                     /locus_tag="OQQ66_000639"
     CDS             complement(10006..10407)
                     /gene="hslR"
                     /locus_tag="OQQ66_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1903675.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10418..11086)
                     /gene="yrfG"
                     /locus_tag="OQQ66_000640"
     CDS             complement(10418..11086)
                     /gene="yrfG"
                     /locus_tag="OQQ66_000640"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1903676.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11151..13286)
                     /gene="igaA"
                     /locus_tag="OQQ66_000641"
     CDS             complement(11151..13286)
                     /gene="igaA"
                     /locus_tag="OQQ66_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1903677.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13606..14166
                     /gene="nudE"
                     /locus_tag="OQQ66_000642"
     CDS             13606..14166
                     /gene="nudE"
                     /locus_tag="OQQ66_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1903678.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14331..16883)
                     /gene="mrcA"
                     /locus_tag="OQQ66_000643"
     CDS             complement(14331..16883)
                     /gene="mrcA"
                     /locus_tag="OQQ66_000643"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1903679.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17003..17782
                     /gene="hofM"
                     /locus_tag="OQQ66_000644"
     CDS             17003..17782
                     /gene="hofM"
                     /locus_tag="OQQ66_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1903680.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17782..18321
                     /gene="hofN"
                     /locus_tag="OQQ66_000645"
     CDS             17782..18321
                     /gene="hofN"
                     /locus_tag="OQQ66_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1903681.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18305..18745
                     /gene="hofO"
                     /locus_tag="OQQ66_000646"
     CDS             18305..18745
                     /gene="hofO"
                     /locus_tag="OQQ66_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1903682.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18735..19139
                     /gene="hofP"
                     /locus_tag="OQQ66_000647"
     CDS             18735..19139
                     /gene="hofP"
                     /locus_tag="OQQ66_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1903683.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19051..20289
                     /gene="hofQ"
                     /locus_tag="OQQ66_000648"
     CDS             19051..20289
                     /gene="hofQ"
                     /locus_tag="OQQ66_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1903684.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20690..21211
                     /gene="aroK"
                     /locus_tag="OQQ66_000649"
     CDS             20690..21211
                     /gene="aroK"
                     /locus_tag="OQQ66_000649"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1903685.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21268..22356
                     /gene="aroB"
                     /locus_tag="OQQ66_000650"
     CDS             21268..22356
                     /gene="aroB"
                     /locus_tag="OQQ66_000650"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1903686.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22448..23734
                     /gene="damX"
                     /locus_tag="OQQ66_000651"
     CDS             22448..23734
                     /gene="damX"
                     /locus_tag="OQQ66_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1903687.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23841..24677
                     /gene="dam"
                     /locus_tag="OQQ66_000652"
     CDS             23841..24677
                     /gene="dam"
                     /locus_tag="OQQ66_000652"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1903688.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24695..25372
                     /gene="rpe"
                     /locus_tag="OQQ66_000653"
     CDS             24695..25372
                     /gene="rpe"
                     /locus_tag="OQQ66_000653"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1903689.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25365..26123
                     /gene="gph"
                     /locus_tag="OQQ66_000654"
     CDS             25365..26123
                     /gene="gph"
                     /locus_tag="OQQ66_000654"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1903690.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26116..27120
                     /gene="trpS"
                     /locus_tag="OQQ66_000655"
     CDS             26116..27120
                     /gene="trpS"
                     /locus_tag="OQQ66_000655"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1903691.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27389..28294
                     /locus_tag="OQQ66_000656"
     CDS             27389..28294
                     /locus_tag="OQQ66_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903692.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28311..28673
                     /locus_tag="OQQ66_000657"
     CDS             28311..28673
                     /locus_tag="OQQ66_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1903693.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28757..29920
                     /locus_tag="OQQ66_000658"
     CDS             28757..29920
                     /locus_tag="OQQ66_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1903694.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            29920..31146
                     /locus_tag="OQQ66_000659"
     CDS             29920..31146
                     /locus_tag="OQQ66_000659"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1903695.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31143..32021
                     /locus_tag="OQQ66_000660"
     CDS             31143..32021
                     /locus_tag="OQQ66_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1903696.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32032..32385
                     /locus_tag="OQQ66_000661"
     CDS             32032..32385
                     /locus_tag="OQQ66_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1903697.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32397..33701
                     /locus_tag="OQQ66_000662"
     CDS             32397..33701
                     /locus_tag="OQQ66_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1903698.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33713..34798
                     /locus_tag="OQQ66_000663"
     CDS             33713..34798
                     /locus_tag="OQQ66_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1903699.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34950..35681)
                     /gene="phoB"
                     /locus_tag="OQQ66_000664"
     CDS             complement(34950..35681)
                     /gene="phoB"
                     /locus_tag="OQQ66_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1903700.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35781..36566)
                     /gene="frlD"
                     /locus_tag="OQQ66_000665"
     CDS             complement(35781..36566)
                     /gene="frlD"
                     /locus_tag="OQQ66_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1903701.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36563..37393)
                     /gene="frlC"
                     /locus_tag="OQQ66_000666"
     CDS             complement(36563..37393)
                     /gene="frlC"
                     /locus_tag="OQQ66_000666"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1903702.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37443..38465)
                     /gene="frlB"
                     /locus_tag="OQQ66_000667"
     CDS             complement(37443..38465)
                     /gene="frlB"
                     /locus_tag="OQQ66_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1903703.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38486..39874)
                     /gene="frlA"
                     /locus_tag="OQQ66_000668"
     CDS             complement(38486..39874)
                     /gene="frlA"
                     /locus_tag="OQQ66_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS1903704.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40119..40286)
                     /locus_tag="OQQ66_000669"
     CDS             complement(40119..40286)
                     /locus_tag="OQQ66_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1903705.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9322 a        10223 c        11078 g         9670 t
ORIGIN      
        1 cgccagcgca tccatcatcg cgctattacc ggcaggctgc gcttcacgac cgcgtggttt
       61 ggctgccggg cggttgtttt gcgggcgttc attaccgccg ccgcgacggg cgttggtttc
      121 gccaggctgc tcgtccagac gcatagtcag ggcgatacgt ttacgctgaa gatccacttc
      181 cagcactttc accttcacaa tgtcgcccgc tttcaccacg gtatgcggat cttccacaaa
      241 cttgttcgac aatgaagaga tgtgaaccag gccgtcctga tgcacgccga tatcgacaaa
      301 cgcgccaaag ttggtgacgt tggttaccgc accttcgagg atcatacccg gttgcaggtc
      361 gttcattgtc tcgacgccat cggcaaactg agcggtttta aattccggac gcggatcgcg
      421 acccggtttt tccagctctt tgatgatgtc agttactgtc ggcacaccga atttttcatc
      481 agtaaagtca gacgctttca ggttacgcag ttcgctgctg ttacccatca gatctttcag
      541 tgcctgctgt gttgctgcca gaatgcgttc caccaccgga taggcttccg ggtggacggt
      601 agaagcatcc agcgggttat caccgtggtt aatgcgcaag aagcccgcgc actgctcgaa
      661 ggctttcggc cccagacggc tgactttcag cagttgctga cggttctgga actggccgtt
      721 ctcatcgcgc caggccacga tgttttgcgc catcatgcgc gtcaggcccg ccacgcgggt
      781 taacagcgga acagaagcag tgttgagatc gacgccaacg gcgtttacgc agtcttctac
      841 tactgcgtcc agtttgcggg ccagttgcgt ctggctgacg tcatgctgat actgacctac
      901 gccgatagat ttcggatcga ttttcaccag ctccgccagc ggatcctgca aacggcgggc
      961 gatagacacc gcgccacgca gcgaaacgtc gagatccggg aactcctgcg ctgccagctc
     1021 ggaagctgag taaaccgacg cgccagcttc gctgacgatc actttctgtg cggtcacttt
     1081 cgggaactgc ttctgcacgt cgagatagaa acgttcagtt tcgcgggaag ctgtaccgtt
     1141 gccgatcgcc accagttcaa cgttatgttt ttcacacaag gcagcaacgg tcatcgctgc
     1201 ttttgcggct tgtccggtgt gcgggtaaat ggtatcggtc gctaccagtt tgccagtggc
     1261 atcgaccacc gccactttta ccccagtacg cagacccgga tcgaggccca tcgttgcacg
     1321 cagtccggca ggggccgcca tcagcagatc gtgcaggtta cgggcaaaga cgttgattgc
     1381 ttcatcttcc gcgcgttcgc gcacggtacc catcagttcg gtttccagat gcatcagcac
     1441 cttgatgcgc caggtccagc tcactacgcc tttgcgccag ctatccgccg gggcattgtt
     1501 caggcgcagg ccaaggtgat ccatgatgat ttgctcgcaa tagctctctt tgggcggctc
     1561 atcgaactgc ggatcggcat tcagcgaaag ctggagtacg ccttcgttac gcccacggaa
     1621 catcgccaga gcgcggtgag aaggcaccgt ggacaacggt tcgtgatgat cgaaatagtc
     1681 gcggaatttc gccccttcct cttctttacc gctcaccacc gtagaaacca aatgcgcgtt
     1741 cttccacaga taatcacgca ctttcgccag cagcgcggca tcttcggcaa accgttccat
     1801 caggatatag cgcgcgccat ccagcgcggc tttggtatct gccacgcctt tatcggcatc
     1861 aacatattgt gcagcggcga cttctggcgt gtgtgacgga tcgctccaca gcaggtcagc
     1921 caacggctca agccctgctt caatggcgat ttgcccgcgg gtgcggcgtt taggtttgta
     1981 gggcaggtag aggtcttcga gttcggtttt gcttagggtg gcgttgatgg ccttcgccag
     2041 atcatcggtg agtttgcctt gctcggaaat ggacttgagg atcgcctgac gtctctcttc
     2101 cagctcgcgc agatagctca gacgcgtttc cagattacgc agctgcgtgt catccagacc
     2161 gccggtgatt tccttacgat aacgtgtgat aaacggcacg gtattccctt cgtcaagcag
     2221 gcgaacggca gcgtcaacct gttccgggcg cgcctgaatt tcacccgcaa taatgcggca
     2281 gaacgaatca ttcatcatgg tttgagttca tcttttcgga tcaaaaatca ggggatagtt
     2341 atacggactg gctggcaaaa atgccagcca tcggcaggag gttaagactc ttccttacgg
     2401 tttcacgtac tcgatagcat taacatacca gctcgcttcc ccggcagggg tattcaccac
     2461 cgccagatcg ccgacttctt ttttcagcaa tgcgcgggcc atcggggaat cgatagagat
     2521 gtaatcttta cggccaaaaa tttcatcgta gccgacaata cggaaacggt gagtcacgcc
     2581 atcgtcgttt tcaatctcca cccacgcgcc aaaaaagact ttgccttcct gctgagggga
     2641 gtaatcgacg attttgagat tttccatgca tttagtgaga tagcgcacgc gacggtcgat
     2701 ttcacgcaga cgctttttat tatactgata gtcagcattt tcgctgcggt cgcccagact
     2761 tgcggcccag gtcacctttt ttgtgacctc cgggcgttct tcacgccaga gataattaag
     2821 ctcttgtttg agtttttcat acccttcccg ggtaaccagg ggcgttttca tctcgttgat
     2881 tccctttgtc tgtttgataa tgcgcacatt gggtataacg tgatcatatc aacagaatca
     2941 ataatgtttc gccgaataaa ttgtatactt aagctgctgt ttaatatgct ttgtaacaat
     3001 ttaggctgaa attcatacca gatttagctg gtgacgaacg tgagcttttt taagaataca
     3061 cgcttacaaa ttgttgcgaa cctttgggag tacaaacaat gcaagagaac tacaagattc
     3121 tggtggtcga tgacgacatg cgcctgcgtg cgctgctgga acgttatctc accgaacaag
     3181 gcttccaggt tcgaagcgtc gctaatgcag aacagatgga tcgcctgctg actcgtgaat
     3241 ctttccatct tatggtactg gatttaatgt tacctggtga agatggcttg tcgatttgcc
     3301 gacgtcttcg tagtcagagc aacccgatgc cgatcattat ggtgacggcg aaaggggaag
     3361 aagtggaccg tatcgtaggc ctggagattg gcgctgacga ctacattcca aaaccgttta
     3421 acccgcgtga actgctggcc cgtattcgtg cggtgctgcg tcgtcaggcg aacgaactgc
     3481 caggcgcacc gtcacaggaa gaggcggtaa ttgctttcgg taagttcaaa cttaacctcg
     3541 gtacgcgcga aatgttccgc gaagatgagc cgatgccgct caccagcggt gagtttgcgg
     3601 tactgaaggc actggtcagc catccgcgtg agccgctctc ccgcgataag ctgatgaacc
     3661 ttgcccgtgg tcgtgaatat tccgcaatgg aacgctccat cgacgtgcag atttcgcgtc
     3721 tgcgccgcat ggtagaagaa gatccagcgc atccgcgtta cattcagacc gtttggggcc
     3781 tgggctacgt ctttgtaccg gacggctcta aagcatgagg cgattgcgct tctcgccacg
     3841 aagttcattt gcccgtacgt tattgctcat cgtcaccttg ctgttcgcca gcctggtgac
     3901 gacttatctg gtggtgctga acttcgcgat tttgccgagc ctccagcagt ttaataaagt
     3961 cctcgcgtac gaagtgcgta tgttgatgac cgacaaactg caactggagg acggcacgca
     4021 gttggttgtg cctcccgctt tccgtcggga gatctaccgt gagctgggga tctctctcta
     4081 ctccaacgag gctgccgaag aggcaggtct gcgttgggcg caacactatg aattcttaag
     4141 ccatcagatg gcacagcaac tgggcggccc gacggaagtg cgcgttgagg tcaacaaaag
     4201 ttcgcctgtc gtctggctga aaacctggct gtcgcccaat atctgggtac gcgtgccgct
     4261 gaccgaaatt catcagggcg atttctctcc gctgttccgc tatacgctgg cgattatgct
     4321 attggcgata ggcggggcgt ggctgtttat tcgtatccag aaccgaccgt tggtcgatct
     4381 cgaacatgct gctttgcagg tcggtaaagg gattattcca ccgccgctgc gtgaatatgg
     4441 cgcgtccgag gtacgttcgg ttacccgcgc ctttaaccat atggcggctg gtgttaagca
     4501 actggcggat gaccgcacgc tgctgatggc gggggtaagt catgacttac gcacgccgct
     4561 gacgcgtatt cgcctggcga ccgagatgat gagcgaacag gatggctacc tggccgaatc
     4621 gatcaataaa gatatcgaag agtgcaacgc catcattgag cagtttatcg actacctgcg
     4681 caccgggcag gagatgccga tggaaatggc ggatctcaac gcagtactcg gtgaggttat
     4741 tgctgccgaa agtggctatg agcgggaaat tgaaaccgcg ctttaccccg gcagcattga
     4801 agtgaaaatg cacccgctgt cgatcaaacg cgcggtggcg aatatggtgg tcaacgccgc
     4861 ccgttacggc aatggctgga tcaaagtcag cagcgggacg gagccgaatc gcgcctggtt
     4921 ccaggtggaa gatgacggtc cgggaattgc gccggaacaa cgtaagcacc tgttccagcc
     4981 gtttgtccgc ggcgacagtg cacgcaccat tagcggcacg ggattagggc tggcaattgt
     5041 gcagcgtatc gtggataacc ataacgggat gctggagctt ggcaccagcg agcggggcgg
     5101 gctttccatt cgcgcctggc tgccagtgcc ggtaacgcgg gcgcagggca cgacaaaaga
     5161 agggtaaata aacgggaggc gaaggtgcct cccgttttgc tttctataag atactggata
     5221 gatattctcc agcttcaaat cattacagtt tcggaccagc cgctaccagc gcggcacccg
     5281 caggggtgtc ggtgtattta tcgaagttgt cgataaacag tttcgccagg gtttcggctt
     5341 tttcctgcca ctgttccgga gaagcgtagg tgttacgcgg atcgagaatc ttcgtgtcta
     5401 cgcccggcag ttcggttggg atcgccaggt taaacatcgg cagagtgaag gtttctgcat
     5461 tatccagcga accgttgagg atggcgtcga taatggcgcg ggtatcttta atcgagatac
     5521 gtttgcctgt gccgttccag ccagtgttaa ccagataagc ctgcgcgccc gccgcctgca
     5581 tacgtttcac cagcacttct gcgtactgag tcgggtgcag cgacaggaat gccgcgccga
     5641 agcaagcgga gaaggttggc gtcggttcgg tgatgccacg ctcagtaccg gccagtttgg
     5701 cggtgaagcc agagaggaag tgatactggg tttgatcggc agtcaggcga gaaaccggcg
     5761 gcaacacgcc gaaagcatca gcagtcagga agataacctt agtcgcgtgg cccgctttgg
     5821 aaaccggctt aacgatgtta tcgatgtgat agattggata agaaacgcgg gtgttctcgg
     5881 tttttgaacc atcatcaaag tcgatagtgc catcttcacg cacggtgacg ttttccagca
     5941 gcgcatcacg acggatagcg ttgtagattt caggttccgc ttctttcgac agcttgatag
     6001 tttttgcgta gcagccgcct tcgaaattaa acacgccgtc atcgtcccag ccgtgttcgt
     6061 catcgccaat caggcggcgt ttcgggtcgg tggaaagggt ggttttaccg gtgccggaaa
     6121 ggccgaagaa caccgcaaca tcgcctttct cgccaacgtt ggcggagcag tgcatagaag
     6181 cgataccttt cagcggcagc aggtagttca tcatcgagaa catccctttc ttcatttcgc
     6241 cgccgtacca ggtgccgcca atcagctgca tgcgctcggt caggttaaac gccacgaagt
     6301 tttcggagtt cagaccctgt tctttccact gcgggttagt gcacttcgcg ccgttcataa
     6361 cgataaagtc tggtttgaaa cctgccagct cttcatcgct cgggcgaata aacatgtttt
     6421 tgacaaaatg cgcctgccag gccacttcgg tgatgaaacg gacggaaaga cgagtatccg
     6481 ggttcgcacc acagaaagcg tcgacaacga acagacgttt gccggaaagc tgcctggtca
     6541 ccaggccttt cagatgctgc caggtttccg gagagagagg tttgttgtcg ttcttacctt
     6601 tgcctttgtc tgcccaccag aaagtatcgc gagtggtatc gtcacggacg atatacttat
     6661 cttttggtga acgaccggtg aagatcccgg tatcgacggc aacggcaccc agattagtta
     6721 acaccccgcg ctcataacct gtcaggctcg gatcgagctc ttcctgatac agcaggtcgt
     6781 agcttgggtt gtaaacgata tcatgtacgt cactgatacc ataagcctcg agttcttgcg
     6841 gggtcaaacc attgttaacg cgcatttcac tgctccttag ccaatatgta ttgcctgaat
     6901 agtaaagtct ttttgggggt gttaaccgcg acaaggctca tagatttacg tatctggaga
     6961 aattcatatt tgacaaaaaa ccccgtgatt cctgttacga aacggttgct attatcgcag
     7021 aaatagcatt ttaggtgtgg aaaatgttct caaaaggtta aatcttggtt tatatggcgg
     7081 gaatattgtg agtggaatcg cattccttaa tgaaattgat gcaaattcaa tcggtaaagt
     7141 atcgattcac cacgctaact ttttttgatt attttctgaa aaaagcaaaa tactactgcc
     7201 cggatgcgat aattaaccca ccttttgatt ctggataacc aacatggata acgttgaact
     7261 ttcaccggca acacgctggg ggatgattgc taccggatta cttcaggggc tggtttgcta
     7321 cctgttgatc gcctggcttg ccggaaaaaa tcacagttgg attgtttatg gcgtgcctgc
     7381 gaccgttgcg ttttcatccg ttttactctt ctccgtgatc tcttttaaac agaagcgcct
     7441 ttggggatgg ctggcgctgg tgtttattgc cacgacaggg atgagcggtt ggctgaagtg
     7501 gcaaactgat ggcatgaacc cctggagagc tgaaaaggcg atttgggatt ttggctgcta
     7561 tctgctgttg atggcaatgt tgttgctacc gtggatacaa caaagccttc gtatccgcaa
     7621 ttgcagtagc cgctatagct acttttacca gtcagtatgg cataacgtac ttatattact
     7681 ggtgattttt ctcgccaatg gcctgacgtg gctggtgctt ttactgtggg gtgagttgtt
     7741 taaacttgtt ggcatcaagt ttttcaatac gctctttttt gcaaccgact ggtttatgta
     7801 tcttacatta ggtctggtta ccgcactggc ggtgattctc gcgcgcacac agtcacgttt
     7861 aatcgactct attcaaaagt tgttcacgtt aatcgccacg gggttgctgc cgttagtatc
     7921 attgctaacc ctgatgttta tcatcactct gccgtttacg ggcctgagcg cgatttctcg
     7981 ccacatctcc gccgccgggt tgctgttaac gctggccttt ttgcaattga tcttaatggc
     8041 tattgtccgc gatccgcaaa aagcgtcact tccctggacg gggccgttgc gttgcctgat
     8101 taaaatcgct ttgctggttg ctccgctgta tgtgttcgtc gccgcctggg cgttatggct
     8161 gcgggtcgct cagtacggct ggactgtcga ccgcttgcag ggcgcgctgg cggtgctggt
     8221 gttactggtg tggtcgctgg ggtattttgt cagtatcgtc tggcgtaatg ggcaaaatcc
     8281 ccttgtcctt cagggcaaag tgaaccttgc ggtttcgtta ttggtgttgg tgatactggt
     8341 gcttcttaat tcgccggtgc tggacagtat gcgtattagc gtgaacagtc atatggcgcg
     8401 ttatcagagc ggcaaaaaca cgccagacca ggtaactatc tacatgctcg aacagagcgg
     8461 tcgctatgga cgtgcggcgc ttgagtcgct gaaaagcgat gccgggttta tgaaagaccc
     8521 gaaacgcgcg cgggatctgc tgatggcgtt agatggagag caacatcttc aggaacaggt
     8581 atcggaaaaa gtattagccg aaaatgtgtt aattgcccct ggttctgtta aaccggatgt
     8641 gacattctgg tcggccttaa tccaggatcg ctataacgtg atgacctgta ttgaaaaaga
     8701 cgcctgcgtc ctggtcgagc aagatctgaa tagtgatggt caggcggagc ggatcctgtt
     8761 tgcttttaat gatgacagag tcattgtcta tggctttgac tcagacagaa aagaatggga
     8821 cgcgcttgat atgagtttac ttccgaacga aataacgaaa gaaaaattac tcacagctgc
     8881 gaaggatgga aaactgggga cgaggccgaa agcgtggcgc gatctgacgg tagatggtga
     8941 aacgctggaa ataaacttaa gtaagtgaca acgcttttat cggatacgac gctgtatctg
     9001 accaaaataa tttgcgattt atttcatagg cgctggtaag cgcagcacat caggccatta
     9061 agttgtaaaa tccggcagag ccctctctgc cggacatact cattaatgaa cttgcggatc
     9121 tgccggagac gcgttgttgc ggatttcagc aatatccatc gcattgaaca gatagtggtt
     9181 accgcagtaa tcacaatgca tgtcaatttc gccatcttcc gccaggatgc tatcaacttc
     9241 ttcatcaggc agcgttttca gcgcatcggc gcaatgttca cgcgagcagg tgcatttgaa
     9301 ctccacatcc tgcggatcgt aaaccgtcac ctcttcttcg tgatacaaac gccacaacac
     9361 ttcgtttgcc ggtaaggtca gcagttcttc ggttttgatg gtttcggtta gcgtcgccag
     9421 gtggtcaaag tcgtcctgct gggcattttg cgcaggcatt acctgcaaca acataccgcc
     9481 tgcagccggt ttgccgtcta cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc
     9541 agaacgcata aagtaatctt ccaggcaggc cgccagggta tcaccttcca gaccaactac
     9601 gccctgatag cgttcgcctt cgctcggggt aatggtgatc accacgtaac cattgccgac
     9661 cagcgttttc aggtcggcat tttctggaat ttcgccctgc acgcgcgcca caccgcgcat
     9721 ttgctggtta ttgttaccgt taataaccgc cagattcatc ggaccgtcgc cctgcagctg
     9781 tacggtgata tcaccatcaa acttcagcgt agcggttaac aggctggtcg caaccagcag
     9841 ttctgccagc acgtttttaa cgggctgcgg ataatcgtgg ttctcaagga tctgttgcag
     9901 ggtttccgaa acggttacca gttcgccgcg aacggcaaaa ttttcaaaca gatagcgatg
     9961 taattggtca tgttgcggca taatcatctc tcttgcaggt gacagttatt cactgtcgcc
    10021 gtgtttaaat cgtaacaggt cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg
    10081 catggttaag gcattaagtt tacgtgccat cgccattttt tcgcgtttct ctacactttc
    10141 cgcagtctct tcatacagca aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc
    10201 ctttacaatc accgtgcgtt cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc
    10261 gacgattttg ctcggcttgc tgcgctgccc gttgtaatgc accttaccgc cttcaatcat
    10321 ttcacgggcc agcgcgcggg ttttataaaa acgggcagcc catagccatt tatccagtcg
    10381 aacctcaaca gcaggtttct ctttcatggc gtctccttca cattagcgag gggatcaggc
    10441 ggcggtagtc attcagtgac ggatggcgtt gatactgttt ctcggcaatc ccggaatcag
    10501 gattagtcac gccgaggcag taacgaatac caaattgcgc ggcagcatcg agaatcgctt
    10561 cgctgtcatc aataaacagc gttctttcag ctttcagacc cgtagcttcg gccaccgcat
    10621 gccataaccg ctgatcctct ttcggataac caaatgtgtg ggtggaaagt aataaatcaa
    10681 ggtgtgcgtc cagacccgta tgctcaagtt ttaccgccag gttgtgcgga tgagcattgg
    10741 tgagcaaaat tcgctgctta ccgctggctt tcagtgcctc aagaaacgga atggtatctt
    10801 cacgcagtac ggcgcgcggt cccatctcgg tggtcattgc acagatatcc agacccagtt
    10861 gctcactcca gtaatcaaga cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct
    10921 ggcgcatata ttccatcgct tcctgtggcg taaccccgtt tttcgcgccc catgtttcag
    10981 gcaccagctt ttgccagaaa tagttatcga aggcgaggtc gagcaacgtg ccgtccatat
    11041 ccagcagaac ggtatctacg tcctgccagg caatgttgat atgcatgagg gaaatctcca
    11101 gagtgaagca atttgcgcga cagggtagca taacctgtcg cgcaaacgtg ttattcgata
    11161 aggctttctg aaggggtgat cagttgcggg ttcaggcagc tttcataata ggcctgaatc
    11221 tccatcatgc gagtgcgatg acgctggtaa cgacgccagg cctgtacgcc attgtaaatg
    11281 gcgctaccca gcatcgtcag tagcagcaat gtggtgctga gatagcgcca caggccggaa
    11341 cgatccggga tcggatgaag gccaatatgc tgcgtaccat tggcgtcagt gaagattttt
    11401 gtgacgatac cttcggcgtt aaacggcgta tgcatcagca tttgtgccag tttctggaaa
    11461 gcgttccact gttcttgcgg cgggtagtcg taaagtgatg ccgaaggcca gggctgatca
    11521 acaaaatcgc tgccttcgtc gctgacaatc aggaatccgc cgggggccgg actgtttagt
    11581 gattgtgccg ctcgcgccgt ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg
    11641 ttatccagcg attccgcact caccgggcgt aataacacat tcacgccatc gagcttcccg
    11701 gcgttggcgc gttttaccag cgcgtcccag tctttactgt tgccgagatt gaccagcgca
    11761 tttttcagtc gcacacagtc atctttggca gaacataaat ccgctgtctt cagtacaatg
    11821 tcgccaaaat catcaagcaa taccatgccg gatttttgaa tcgctgaacg taatgaggca
    11881 ctgacgcgag attcatcttc cggtttaggg tgcagctggc gattaactgc ttcagtcaat
    11941 gccgtcgctt tgttgaccag ttcagattct ggtaatggta atgagcgggc gtcattccag
    12001 atgatctgcg agcagtcaaa cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg
    12061 gaagtgcgaa tattacacat tcccgtaccg ctaatacgca atgtatcgcc tacccgcacg
    12121 ccagcgtcag ccagttgttt tacgctggtg gcttcaatgg tctgcgcgcc tttcatccat
    12181 gagagagtga atttcagcgg catatccagc gggatccaga ataacagcat aaacagcacc
    12241 agcagcgagc ccgcagcgat aatcgtactg cgcagccagt gctgtaacgg aaagtttttt
    12301 acttcatcat gcagcgaaag atatcgcccc tgacgcacta cgtggcagtc gagatagata
    12361 tcaatatcgg tttgttgatc gagatcctga gcaatgtatg gctgccagtg tgcgggatag
    12421 accaggtcga taataccgag tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac
    12481 aatccccaac gacggggtgt accgcgcaga caatgaattt cccgcaggga ggattttgcc
    12541 gggggcgcga atagccccca cagacctgcg cccagcagca gtaatgcgcc gcctgccagc
    12601 cacggaacaa atacatccgg ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg
    12661 atcagcaacg cttcacgcag cccgcgcgga cgactcagcg catattcttc atgcgtttct
    12721 ttgcgaatat tgagtagctc gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg
    12781 gacggaacag gttgcaaggc atagccgcgc gtttcctgca tgtactcctg tagcgtatga
    12841 ccgttgagtg aaataaccaa cggcagcgaa tcggtatgaa tcagttcaac ggtattctca
    12901 tcgttgatgt actgttccca gaaggggggc aggtggactt ctaccgaatc gaggtagtaa
    12961 cgccatttat tcggatcgtc ggtagagatg ccgtaacgtg tgatagcgtg tgtcagcatc
    13021 atgacgttgt tgctttcggc attcagcgcc agagagatcg gtgccgcgct agctcccgtt
    13081 gggccaggca cctgtaatac ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt
    13141 tcttcaggtg tgagtttacg cgtttgcgca tccgcgaagg cgttggtcca gggcagctga
    13201 ctccgtctgg atcgcacttt tatcagccat cccgcaagta gtgagcaggc cagcaaagca
    13261 gctaaaaaaa tcacaatggt gctcatgctt tccccatctt acttagtctg tcaggcgtgg
    13321 tcagtcgcac cagacacaat acatgattct gcggttggct atcggcaagc gtggagttta
    13381 acttgagcat cattaagcac atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg
    13441 gaatatcagc aaattcctag agttatttca acctattgac ttttaatttg atttgaggac
    13501 gattatttat taagttacat aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt
    13561 ccgatatcgc acaataaccg ctgatttcac agcatagatc ccacgatgag caaatcatta
    13621 caaaaaccca ccattctgaa tgttgaaact gtagcccgtt cccgactgtt taccgtcgag
    13681 agcgtggatc tggagttcag caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc
    13741 aaccgggaag cagtgatgat tgtgccgatt gtggacgatc acctgatcct gatccgcgaa
    13801 tacgcagtgg gaactgaatc ctacgaatta ggtttttcga aaggattaat tgatccgggt
    13861 gaaagcgtct acgaagccgc taaccgcgag ctaaaagaag aggttggatt tggtgcgaac
    13921 gatctgactt ttttgaagaa gctcagcatg gcaccgtctt acttttccag caaaatgaat
    13981 atcgtggtcg cgcaagatct ctacccggaa tcgctggaag gcgatgagcc agagccgcta
    14041 ccacaggtgc gctggccgct ggcgcatatg atggatttgc tggaagaccc tgacttcaac
    14101 gaagcgcgta atgtcagcgc gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga
    14161 gtgtaaatgc gtgaagtttg cctgatgcac aacgcttatc aggcttatgg ggttcctgca
    14221 atatattgat ttttcacgat tttgttagcc gggtaacgcg gtcacccgca tccagcataa
    14281 acaaatgcac tttgtcagcc aactgaaaag gcgccgaagc gcctttttaa tcagaacaat
    14341 tcctgtgcct caccattatc gataatggtc gttcccacct cgtgcaccgc ctgttgtgtc
    14401 ggctgcgtac cttcgatgaa atactcttcg cggctgttgc caccattagc taactgcccg
    14461 gtgctgcgat cgatattcac cgtcacaata cccggtggcg gcgtcagcgg ctgctccggc
    14521 acaccttcaa gaacggcttt catataagcg tcccatgcag gctgggcact cttggctccg
    14581 ccttcgtaac ctgagatctg atctttaatc gctccggaag ccgttgtatg accgagatta
    14641 cgacggtgat catcaaagcc aatccagacc gaggtcacaa cgcccggacc gtaacccgag
    14701 aaccacgcat ctttcgaact gttagtggta ccggttttcc cgccgatatc gcgacgctgc
    14761 aaatcacgac ctgcacgcca gccagtaccc tgccagcctg gctcaccaaa gatattggtg
    14821 ttcaaagcac tcttaatcag gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc
    14881 tgcgcgccag tcttcgccac taacgcctga tttgcctgct ccagctgcgg cattggtaca
    14941 gaaacattct gctgctcgcg ggagatagcg acatcttcaa catcgttatt ttccagcacg
    15001 ttcgatttct gcgtatcacc gtaaatcacc ggaatatcgc attccgggca ggctactttc
    15061 ggtttcgctt cgaaaatcac gccgccctga tcgttttcaa ttttgctgat aaaccacggg
    15121 tccaccagga agccgccgtt cgccatgacc gcgtagccgc gcgccacctg cattggggtg
    15181 aaggacgctg aacccagcgc cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg
    15241 aagcgttgca gatattctgc agcgtagtcg acgcccatag cccgcattgc acgtaccatc
    15301 accacgtttt tcgactgacc cagcccctga cgtaagcgaa ttggaccagc atattgtggc
    15361 ggcgagtttt tcggctgcca gtcagaaccg gcacctgcgt cccagcgaga aattggcaca
    15421 tcgttcaaca tacttgccag cgtcagacct ttatccatcg ccgcggtgta gaggaacggt
    15481 ttgatgttgg aacccacctg acgcagtgcc tgggtggcgc ggttaaactt gctctgattg
    15541 aaatcaaagc caccgaccag cgccataacg gcaccgtttt gcggattgat cgacaccagc
    15601 gccgagttca cttccggcac ttgtgccagc caccatgcat cgccaacctg acgaacccag
    15661 atttgctgac ccgtttgcag aacatcggtc actttacgcg gcgtcggtcc ctgctgagta
    15721 tccgaacggt aaggacgcgc ccagcgaacg ccttccatac tcaatgcgac ggtcgacccg
    15781 tccgccagca tcgccgtcgc tccctgagga ttggcgctgg tgactgcggc aggcagcagc
    15841 ggaccatagg tcggcagcgc cttcagcgta tcggtaatct tgttgttatc ccacgccgac
    15901 tcgcccactt tccacagcac atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc
    15961 agcacgttat tacgtaccgc ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg
    16021 taaatgcgat aaccgtcttc ataggcactt tcgccataac ggttatacat ctcctggcgc
    16081 accatttcgc tcaggtacgg cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta
    16141 atcgcctcag tgcgtgtctg atcgaactgt tgttgggtga tatacccttc atccagcatc
    16201 cgcgacagta cgacgttacg ccgcgcgacg gcacgatcca ttgagtagag cggattaaag
    16261 gtggaaggcg ctttcggcag cccggctatc acagccattt cgttcagcgt cagttggtcg
    16321 accgttttac cgaaatagac ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg
    16381 taaatcttat tcagataaag ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga
    16441 atcgcgagga agacttcctt aatcttacgc atcagcgtgc gttctggact gaggaagaag
    16501 tttctcgcca gctgctgggt aatggtactc gccccttgtg acgcgtggcc ggagaacagc
    16561 gccacacttg ccgcacggaa gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg
    16621 ctgtcttccg tcgcgataaa ggctttcacc atctccggcg ggatttgatc caacgtaacc
    16681 ggaatacgac gtttctcacc gtattgagca atcagctcgc catcggcgct gtaaatctgc
    16741 atcggaattt gcaggcgaac atcttttaat gtcgccacat ccggcagttg tggctcgatg
    16801 tagcggtata ggccataaat cgagcctgct cccagcagaa tgcaacagac tgcaaggatc
    16861 aaaaaatact ttacgaactt cactggaaat ttcccattta gtttcatttg ggcagtttat
    16921 aaacaaacgc gcggtagtat aaaggcaagc cagacgcatt gatatacccg tcagagtgac
    16981 gggtgataag gagatcatca caatggcatt taagatctgg caaattggtt tgcatttaca
    17041 acagcaagaa gcggtagcgg ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg
    17101 ctggtggcgg ttgccgctgg agaacgacat tatcaaagat gggcggattg ttgatgcgca
    17161 gcagctggct aaaacgttgt taccttggag tcgcgaactg ccgcagcgtc atcacattat
    17221 gttggcgttt cccgccagtc gcacattaca gcggtcattt ccgcgcccgt cgatgtccct
    17281 tggtgagcgg gagcaaacgg cctggctgtc agggacgatg gcccgcgagc tggatatgga
    17341 tccggactcc ctgcgcttcg attacagcga agactcactc agtcccgcct ataacgtgac
    17401 tgccgcgcaa agcaaagagc tggcaacgct gcttactctg gcagaaaggt tgcgtgttca
    17461 tgttagtgcg atcaccccgg atgccagtgc attacagcga ttcctgcctt ttttaccttc
    17521 tcatcagcaa tgtctggcct ggcgtgataa cgaacagtgg ctgtgggcga cgcgctatag
    17581 ctgggggcgc aaactggcgg tggggatgac tagcgcgaag gagctggcgg cagcgttatt
    17641 cgttgatccc gacagcgtcg cgatatgtgg cgaaggcgga tttgacccct gggaggccgt
    17701 ttctgttcgt cagccgctgc taccgccgcc tggtggagac tttgccatcg cgctggggct
    17761 ggcgcttggg aaggcgtact gatgaacccg ccaattaatt ttttgccctg gcgacagcaa
    17821 cgccggaccg cttttctgcg tttctggttg ctgatgttcg ttgcgcctct gctgctggcc
    17881 gtcgggataa cgctaatact gcgtctgaca ggcagcgccg aagcgcgcat agacgcagtt
    17941 ttgcttcagg cggaacaaca actcgcccgc agcttacaga taacgaagcc acgtttgctg
    18001 gagcaacagc agttacgcaa acagcgttcg caacggcagc gccagcgaca atttacccgc
    18061 gactggcaat ctgcgctgga agcactggcg gcgcttttac ccgagcacgc ctggctgaca
    18121 acgataagct ggcagcaggg aacgctggag atcaaggggc tgacaacaag cattaccgcg
    18181 ttaaacgcac tagaaacgtc actccgccag gatgcgtctt ttcatctcaa tcagcgggga
    18241 gccacgcagc aggatgcgca gggacgctgg caatttgagt atcagttaac aaggaaggtt
    18301 agcgatgaac atgttctttg actggtggtt cgccacatca ccccgcctcc gccagttttg
    18361 ctgggcattc tggttgctga tgttagttac gctcattttt ctgtcatcga cacaccatga
    18421 agagcgcgac gcattaattc gattacgggc aagtcatcac cagcagtggg ccacactgta
    18481 tcgcctggtt gacaccactc ccttcagcga agaaaaaacg ctgccctttt cgccactgga
    18541 ttttcagttg cccggcgcgc aactggtttc ctggcatcca tccgcgcagg gaggtgagtt
    18601 ggcgttgaaa acgctgtggg aagcagtgcc gtcggcattc acacggctgg cagagcgcaa
    18661 cgtcagcgtg agccgttttt cgttaagcgt ggaaggtgat gatcttttgt tcacgctaca
    18721 actggagacg ccgcatgagg gttaaacgct ggttgttggc aggtattgca ttgtgccttt
    18781 taaccggtat gcgtgaccct tttaaaccgc cggaagatct atgccggatt agcgaactta
    18841 gccagtggcg ctatcagggg atggtagggc gaggcgagcg catcatcggt gtaataaaag
    18901 acgggcaaaa gaaatggcga cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa
    18961 ttttacagct gacgccagac gtactaacgc tgggtaccgg gacaaactgc gaaccgccac
    19021 aatggttgtg gcaacggcaa ggagatacaa atgaagcaat ggatagccgc actactgttg
    19081 atgctgatac ccggcgtaca ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac
    19141 gttccggtag ctcaggtgtt gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg
    19201 tcgccagacg tcagcggtac ggtgtcgtta catctaacag atgttccctg gaagcaggca
    19261 ctacaaactg tagtgaaaag cgccggactg ataacgcggc aggaaggcaa cattctctca
    19321 gtgcattcca ttgcctggca gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg
    19381 gcgcaggcaa atctgccgct ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga
    19441 gaactggcga aagcggggga gaagctactg agtgccaaag ggagtatgac cgtcgataaa
    19501 cgcaccaatc gccttttgct acgagataac aaaacggcgt taagcgcgct tgaacagtgg
    19561 gtagcgcaaa tggatctgcc ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt
    19621 aatgaaaaaa gtttgcgtga gttaggcgtg aaatggacgc tggccgatgc gcaacacgct
    19681 ggtggcgttg ggcaagtcac cacgcttggt agcgacctct ccgtagcgac ggcgacaacg
    19741 catgtcggtt ttaacattgg gcgcatcaac ggacgcttgc tggatcttga gctttccgcg
    19801 ctcgaacaaa aacagcagct ggatattatc gccagtccgc gtctgctggc ctcacatctt
    19861 cagcctgcca gcattaaaca ggggagcgaa attccatatc aggtttccag cggggaaagt
    19921 ggcgcgacgt cggtggaatt taaagaggcc gtcctgggga tagaggtcac gcccacggtg
    19981 ttacaaaaag gtcgcatccg gctgaaatta cacatcagcc agaacgttcc ggggcaggtg
    20041 ctacagcagg ccgatggcga agtgctggcg attgataagc aggagatcga aacgcaggtc
    20101 gaggtcaaaa gcggagaaac gttggcgctg ggcggcattt ttacccgtaa aaataaatcg
    20161 ggtcaggata gcgtaccgtt gcttggcgac attccctggt tcgggcaatt atttcgtcat
    20221 gacggaaaag aatatgaacg acgcgagtta gtggtgttta tcacgccacg actggtttcc
    20281 agtgagtaaa cagccgtaaa agcggtaatg tttttatgct gaacgtgttt catctatttg
    20341 acgcgcgcag gtatttagca tacaaggagt accgatttga gagttggcgc tcttcgctgc
    20401 ctgcgttcca tgatgatgat ttatcattca ggcggcattt tgctgtcttt tttacgctaa
    20461 tcttacccgg tgatttatcg ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca
    20521 gatatgcaga gggatgggtg atttattcag ttgccaaacc cgctggagta ttgagataat
    20581 tttcagtctg actctcgcaa tatcttatga ggtttcagtt catgtcctgc ggcgctctct
    20641 gagcgaagcg ggtttatcat taacgaatag tcttagtagt accgaaaaaa tggcagagaa
    20701 acgcaatatc tttctggttg ggcctatggg tgccggaaaa agcactattg ggcgccagtt
    20761 agctcaacaa ctcaatatgg aattttacga ttccgatcaa gagattgaga aacgaaccgg
    20821 agctgatgtg ggctgggttt tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga
    20881 aaaggtcatc aatgagttga ccgagaaaca gggtattgtg ctggctactg gcggcggctc
    20941 tgtgaaatcc cgtgaaacgc gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga
    21001 aacgaccatc gaaaagcaac ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca
    21061 cgttgaaaca ccgccgcgtg aagttctgga agcgttggcc aatgaacgca atccgctgta
    21121 tgaagagatt gccgacgtga ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa
    21181 ccagattatt cacatgctgg aaagcaacta attctggctt tatatacact cgtctgcggg
    21241 tacagtaatt aaggtggatg tcgcgttatg gagaggattg tcgttactct cggggaacgt
    21301 agttacccaa ttaccatcgc atctggtttg tttaatgaac cagcttcatt cttaccgctg
    21361 aaatcgggcg agcaggtcat gttggtcacc aacgaaaccc tggctcctct gtatctcgat
    21421 aaggtccgcg gcgtacttga acaggcgggt gttaacgtcg atagcgttat cctccctgac
    21481 ggcgagcagt ataaaagcct ggctgtactc gataccgtct ttacggcgtt gttacaaaag
    21541 ccgcatggtc gcgatactac gctggtggcg cttggcggcg gcgtagtggg cgatctgacc
    21601 ggcttcgcgg cggcgagtta tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta
    21661 ctgtcgcagg tcgattcctc cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa
    21721 aacatgattg gcgcgttcta ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa
    21781 acgcttcccc cgcgtgagtt agcgtcgggg ctggcagaag tcatcaaata cggcattatt
    21841 cttgacggtg cgttttttaa ctggctggaa gagaatctgg atgcgttgtt gcgtctggac
    21901 ggtccggcaa tggcgtactg tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc
    21961 gccgacgagc gcgaaaccgg gttacgtgct ttactgaatc tgggacacac ctttggtcat
    22021 gccattgaag ctgaaatggg gtatggcaat tggttacatg gtgaagcggt cgctgcgggt
    22081 atggtgatgg cggcgcggac gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag
    22141 cgtattataa ccctgctcaa gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc
    22201 gcgcaggcgt atttaccgca tatgctgcgt gacaagaaag tccttgcggg agagatacgc
    22261 ttaattcttc cgttggcaat tggtaagagt gaagttcgca gcggcgtttc gcacgagctt
    22321 gttcttaacg ccattgccga ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta
    22381 tcaagcggtc tattagcttc aggttaattg caacgtggta agcattaacc ttttagtggg
    22441 gtgttaaatg gatgaattca aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg
    22501 tactggtcgt tctcgtcaat cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca
    22561 gatcaatttt gatgatattg aacttgatga cactgacgat cgccgtccga ctcgtgcgca
    22621 aaaagagcgc aatgaggaac cggaaatcga agaagaaatt gacgaatccg aagatgaaac
    22681 cgtggatgaa gagcgcgtag agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc
    22741 cgcttctcgt cagtatatga tgatgggcgt cggcattctg gttctactgc tgttgatcat
    22801 cggtatcggt tctgcgctaa aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg
    22861 cgataagagt attgatcttg caggcaatgc gaccgatcag gcgaatgggg tgcagccagc
    22921 gccgggaacc acgtctgcgg aaaatactca gcaggatgtt tctctgccac cgatctcttc
    22981 tacgccgact caagggcaaa ccccggcggc aacggatggt caacaacgtg ttgaagtgca
    23041 gggtgacctg aacaatgcgc tgactcagcc acaaaatcag caacagttga acaatgtggc
    23101 ggtcaattcc acattgccga ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc
    23161 atcgcgtgac acggcgaaaa cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg
    23221 tcagcaggcg gtgattgaac cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc
    23281 ggtagcacag acgccgaagc gtactgaacc agctgcccct gtggcgagca cgaaggcacc
    23341 ggctgcgact tctacgccag caccaaaaga gacggcgact acggctccag tacagacggc
    23401 atccccggcg caaaccacgg caacaccagc cgctggaggg aagaccgcag gtaatgttgg
    23461 ttcgttgaaa tcggcaccgt ccagccatta cactctgcag ctgagcagtt cctctaacta
    23521 cgacaacctg aacggttggg cgaagaaaga gaatctgaaa aactacgttg tctatgaaac
    23581 gacgcgtaat ggtcagccgt ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga
    23641 ggcgaaaaaa gcggtatcta cattgccagc agatgttcag gccaaaaacc cgtgggcgaa
    23701 accgctgcgt caggtacagg ccgatctgaa gtaatcaagg ttatctcccg caatggttta
    23761 tcgttgcggg agctgcctga agcgctggat gctgtcggag ctttctccac agccggagaa
    23821 ggtgtaatta gttagtcagc atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca
    23881 agtatcccct gcttgatgat attaaacggc atttgcccaa gggcgaatgt ctggttgagc
    23941 cttttgtagg tgccgggtcg gtgtttctca acaccgactt ttctcgttat atccttgccg
    24001 atatcaatag cgacctgatc agtctctata acattgtgaa gatgcgtact gatgagtacg
    24061 tacaggccgc acgcgagctg tttgttcccg aaacaaattg cgccgaggtt tactatcagt
    24121 tccgcgaaga gttcaacaaa agccaggatc cgttccgtcg ggcggtactg tttttatatt
    24181 tgaaccgcta cggttacaac ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc
    24241 cgtttggccg ctacaaaaaa ccctatttcc cggaagcaga gttgtatcac ttcgctgaaa
    24301 aagcgcagaa tgcctttttc tattgtgagt cttacgccga tagcatggcg cgcgcagatg
    24361 atgcatccgt cgtctattgc gatccgcctt atgcaccgct gtctgcgacc gccaacttta
    24421 cggcgtatca cacaaacagt tttacgcttg aacaacaagc gcatctggcg gagatcgccg
    24481 aaggtctggt tgagcgccat attccagtgc tgatctccaa tcacgatacg atgttaacgc
    24541 gtgagtggta tcagcgcgca aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca
    24601 acggcggcac acgtaaaaag gtggacgaac tgctggcttt gtacaaacca ggagtcgttt
    24661 cacccgcgaa aaaataattc tcaaggagaa gcggatgaaa cagtatttga ttgccccctc
    24721 aattctgtcg gctgattttg cccgcctggg tgaagatacc gcaaaagccc tggcagctgg
    24781 cgctgatgtc gtgcattttg acgtcatgga taaccactat gttcccaatc tgacgattgg
    24841 gccaatggtg ctgaaatcct tgcgtaacta tggcattacc gcccctatcg atgtacatct
    24901 gatggtgaaa cccgtcgatc gcattgtgcc tgatttcgct gccgctggtg ccagcatcat
    24961 tacctttcat ccagaagcct ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa
    25021 tggctgtaaa gcgggtctgg tatttaaccc ggcgacacct ctgagctatc tggattacgt
    25081 gatggataag ctggatgtga tcctgctgat gtctgttaac ccaggttttg gcggtcagtc
    25141 gttcattcca caaacactgg ataaactgcg cgaagtacgt cgtcgtatcg acgagtctgg
    25201 ttttgacatt cgactggaag tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc
    25261 tgcggcgggc gcggatatgt tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa
    25321 aaaagtcatt gatgaaatgc gcagtgaact ggcaaaggta agtcatgaat aagtttgaag
    25381 atattcgcgg cgtcgctttt gatctcgacg gtacgctggt tgacagtgct cccggtcttg
    25441 ctgctgcggt agatatggcg ctgtatgcgc tggagttacc cgtcgcaggt gaagaacgcg
    25501 ttattacctg gattggtaac ggcgcagatg ttctgatgga gcgtgcattg gcctgggcgc
    25561 gtcaggaacg tgcgactctg cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc
    25621 cggcagaaga acaggtacgt attctgcgta aactgttcga tcgctactat agcgaggttg
    25681 ccgaagaggg gacgtttttg ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta
    25741 aaggcctgcc gctaggcctg gtcaccaaca aaccgacgcc gttcgtcgcg ccactgctcg
    25801 aagccttaga tattgccaaa tactttagcg tggttatcgg cggcgatgat gtgcaaaaca
    25861 aaaaaccgca tccggacccg ctgttactgg tggctgagcg gatgggaatt gccccacaac
    25921 agatgctgtt tgtcggcgac tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc
    25981 catcagttgg cttaacctac ggatataact acggcgaggc tatcgatctc agccagcctg
    26041 atgtaattta tcagtctata aatgaccttc tgcccgcatt agggcttccg catagcgaaa
    26101 atcaggaatc gaaaaatgac taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa
    26161 ttgaccattg gtaactacat gggtgcgctg cgtcagtggg taaacatgca ggatgactac
    26221 cattgcattt actgtatcgt tgaccaacac gcgatcaccg tgcgccagga tgcacagaag
    26281 ctgcgtaaag cgacgctgga tacgctggcc ttgtatctgg cttgtggtat cgatcctgag
    26341 aaaagcacca tttttgttca gtcccacgta ccagaacatg cgcagttagg ctgggcactg
    26401 aactgctata cctacttcgg cgaactgagc cgcatgaccc agtttaaaga taaatctgcg
    26461 cgttatgccg agaacatcaa cgctggtctg tttgactatc cggtgctgat ggctgcggac
    26521 atcctgctgt atcaaactaa tctggtaccg gtgggtgaag accagaaaca gcacctggaa
    26581 ctgagtcgcg atatcgccca gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg
    26641 gagccgttta ttccgaaatc tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag
    26701 atgtccaagt ctgacgataa ccgcaataac gttatcggcc tgctggaaga tccgaaatcg
    26761 gtagtgaaga aaatcaaacg cgcggtcact gactccgacg agccgccggt agttcgctac
    26821 gatgtgcaga acaaagcggg cgtttccaac ctgctggata tcctttctgc ggtaacgggc
    26881 cagagcatcc cagaactgga aaaacagttc gaaggcaaga tgtatggcca tctgaaaggt
    26941 gaagtggctg atgccgtttc cggtatgctg actgaattgc aggaacgcta tcaccgtttc
    27001 cgcaacgatg aagccttcct gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta
    27061 cacgcttccc gtacgctgaa agcggtgtac gaagcgattg gttttgtggc gaagccgtaa
    27121 tatcaaaacg ctcggacggt cccctcgccc ctttggggag agggttaggg tgaggggagc
    27181 aggcaagcgc cagtgggaat taccctttgc tcgcattgtc gaataaatca cttcgtcatc
    27241 actctgaaac cggggaaacc cggttttttt atcctcattt gcaataatcc gaaaaaatgt
    27301 gaagcgcctc gccgtttcca catctcacag ttgccactta tcttcatttt aatgaagata
    27361 taaacattca ttttattgaa aatatattat gcgtcgaacg tttatcaaaa aagaaggcgt
    27421 cgtcatcacg acgctggccc gttatttgtt gggtgaaaaa tgcggtaatc gactgaaaac
    27481 catagatgag ctggcaactg aatgccgttc atcggttggc ctgacgcagg ccgcgttgaa
    27541 aacgttggaa tcaagtggag cgatacggat tgaacgccgt gggcgcaatg gcagttatct
    27601 ggtcgaaatg gataacaaag cgctgctgag tcatgtggat atcaataacg ttgtgtgtgc
    27661 catgcctttg ccctataccc gcttgtacga aggtttagca agcgggctga aagcccagtt
    27721 tgatggcatt cctttttatt atgcgcatat gcgtggcgcg gatattcgtg tggagtgttt
    27781 gcttaacggt gtctatgaca tggcagtagt ttcccgcctg gcggcggaaa gttatctcag
    27841 ccaaaacaat ttgtgcatcg ccctcgagct tggcccgcat acgtatgttg gtgaacatca
    27901 actgatatgc cgcaaaggtg agtctggaaa tgttaagcga gtaggtctcg atagtcgttc
    27961 ggcggatcag aaaatcatga ccgatgtctt ttttggcgat agtgatgtgg aacgtgtcga
    28021 tctctcttat cacgagagtt tacaacgcat tgttaaaggc gatgtcgatg cggttatctg
    28081 gaacgtggtg gcggaaaacg aactgaccat gctgggatta gaggcgacgc cgctcacaga
    28141 cgatccgcga tttttacagg ccaccgaagc cgtggtcctg acgcgagtcg atgattaccc
    28201 aatgcaacaa ctgctgcgtg ccgttgtaga taaacacgcc ctgctcgccc atcaacaacg
    28261 ggtagtgagt ggggaacagg aaccgagtta ttaaacgaaa ggcatctgat atggaaacca
    28321 gactcaacct gctttgcgag gcaggcgtta ttgataagga catctgcaaa ggcatgatgc
    28381 aggtcgtcaa cgtactggaa acagagtgcc atctgccggt gcgcagtgag caaggaacga
    28441 tggcgatgac acatatggcg agtgcactga tgcgcagtcg ccgtggtgaa gaaatagagc
    28501 cgctggataa cgaattgctg gcagagctgg cgcaatccag ccactggcaa gccgttgtgc
    28561 aattgcatca ggtgttgttg aaggaattcg cactggaagt taatccgtgt gaagaaggct
    28621 atttgctggc caacctttat ggattatgga tggctgctaa cgaagaggtt tgaagctccc
    28681 gacaaatcca cgtcgcacaa cctaaatatt caaaaaaatg aatatttaat tcaaggaaca
    28741 gaaacgaggc aacaagatgt ttgtagaagc attgaaacgc cagaacccgg cgctgatttc
    28801 cgccgcacta agtttgtggc agcagggcaa gatcgccccg gacagctggg tgatcgacgt
    28861 ggatcaggta ctggaaaacg gtaagcgact gattgagacg gcgcggcttt acggcattga
    28921 actgtatctg atgaccaagc agtttggccg taacccatgg ctggcggaaa aattgctggc
    28981 attaggttac agcggcattg tggcggtgga ttacaaagag gcgcgagtca tgcgccgcgc
    29041 tggtttgtcg gtggcgcatc aggggcatct ggtacaaatc ccttgtcatc aggttgctga
    29101 cgccgttgaa cagggcaccg acgtcatcac cgtgtttact ctcgacaaag cgcgggaagt
    29161 ttctgcggca gcggtgaagg ccggacgtgt gcagtttgtg ctgctcaaag tttatagcga
    29221 cgatgatttt ctttatccgg ggcaggagag cggttttgta cagcattcgc tgcacgaggt
    29281 ggttgctgaa attaagaaac tgccgggtct gcatttagcc ggacttaccc atttcccttg
    29341 cctgctttgg gatgaggctg ccggaaaagt tttgccgaca ccgaatcttc acacgctgat
    29401 acaggcacgg gatcaactgg cgaaatctgg tattgcactt gagcaactga acgcgccttc
    29461 agcgaccagc tgcacttcgc tgccattact ggcgcaatac ggtgtgactc atgccgaacc
    29521 cggtcatgcg ctgacgggca ctattccggc aaaccagcag ggcgatcagt ctgaacgtat
    29581 cgcgatgctc tggttgagtg aaatctccca tcatttccgt ggcgacagct actgctacgg
    29641 cggcggttac taccgtcgtg gtcatgcgca acatgcgttg gtgtttacgc cagaaaatca
    29701 aaagattact gaaaccaatc tcaaaactgt ggatgacagc agtatcgact acaccctgtc
    29761 gctggcaggc gagtttccgg taagcagtgc agtggtgctc tgtttccgca cgcagatttt
    29821 tgtcacccgt agcgatgtgg tgctggtgtc cggtattcac cgtggcgaac cgaaaatcgt
    29881 tggtcgttat gacagtcttg gaaactctct gggggcgtaa tggcgcgatt tgtggtgtta
    29941 gtgattgata gctttggcgt aggggcaatg aaagatgtca cgctggtgcg tccgcaagat
    30001 gcgggagcga atacatgtgg tcacatcctg agccagttgc cgcatttgca gctaccaacg
    30061 ctggagaagc tggggctaat caacgcattg ggttatgcgc caggcgatat gcagccgtca
    30121 gattccgcaa cctggggcgt ggcagagctg caacatgaag gtggcgatac ctttatgggg
    30181 catcaggaaa ttttaggcac gcgcccgtta ccgccgctgc ggatgccttt ttgcgatgtg
    30241 attgaccgtg ttgagcaggc attagtatcc gccggttggc aggtggagcg ccgtggcgat
    30301 gaactgcaat ttctgtgggt caatcaggcg gttgcgattg gcgataatct cgaggcggat
    30361 ttaggccaag tctataacat taccgccaat ctctctgtga tctcttttga cgacgcaatc
    30421 aaaattggtc gtatcgtgcg tgagcaggta caggtcggtc gggtcattac atttggtggc
    30481 ctgttaaccg acagtcaacg cattctcgat gccgcagaaa gcaaagaagg gcgctttatt
    30541 ggtatcaatg cgccgcgttc tggcgcttat gacaacggct tccaggtcgt gcatatgggc
    30601 tacggtgtcg ataaaaaagt gcaggtgccg caaaaactgt atgaggcggg cgtgccaacc
    30661 gtgctggtgg gtaaggtggc agatatcgtc agcaatcctt atggcgtgag ctggcaaaac
    30721 ctggtggata gccagcggat tatggatatc accctcgacg aatttaacac ccatccgacg
    30781 gcgtttattt gcaccaatat ccaggaaacc gacctcgctg gtcatgcaga agacgtcgca
    30841 cgttatgccg aacgtttgca ggtcgttgac cgtaaccttg cccggcttgt tgatgcgatg
    30901 cagccagatg attgcctggt cgtgatggcg gatcacggca acgatccgac cattggtcac
    30961 agccaccata cccgcgaagt ggtgccgctg ctggtttatc agcaaggcct ggctgctacg
    31021 cagctcggtg tgcgcaccac gctttctgat gtgggagcta ccgtgtgtga atttttccgt
    31081 gcgccaccgc cacaaaatgg tcgctctttt ctttcctccc tccggtttgc aggagacacc
    31141 ctatgagttt tgatccgacg ggttacaccc tggcccatga gcatctgcat attgatctct
    31201 ccggctttaa aaacaacgtg gactgccgcc ttgatcagta tgcgttcatt tgccaggaga
    31261 tgaacgacct gatgacccgg ggtgtacgta atgtgattga gatgaccaac cgttacatgg
    31321 ggcgcaatgc gcaatttatg ctcggtgtaa tgcgcgaaac cgggatcaac gtggtggcct
    31381 gtactggtta ttaccaggat gcgtttttcc cggaacatgt ggtgacccgc agcgtgcagg
    31441 aactggcgca ggagatggtc gatgaaattg aacagggtat cgatggcacg gagctgaaag
    31501 ccgggctcat cgcggagatc ggctccagcg aaggaaagat tacgccgctg gaagagaagg
    31561 tatttattgc cgctgcgctg gcgcataacc agaccggacg cccgatctcc acgcatacgt
    31621 cgttcagcac catggggctg gaacaactgg ctctgttgca agcccacgga gttgatcttt
    31681 cgcgcgtcac cgttggtcac tgcgatctga aagacaacct cgacaacatt ttgaagatga
    31741 tcgatctcgg cgcgtacgtg cagttcgaca ccattggtaa gaacagttac tacccggacg
    31801 aaaagcgcat tgcgatgctc cacgcgctac gtgaccgtgg gttgctgaac cgcgtcatgc
    31861 tgtcgatgga tattacgcgc cgctcccatt taaaagccaa tggtggttat ggctatgact
    31921 atttattgac cacctttatt ccgcaattgc gccagtcagg attcagtcag gccgatgtgg
    31981 atgtgatgtt acgtgaaact ccctctcaat ttttccaata aggacagact catgaaaaag
    32041 attggcgttg caggcttaca gcgtgagcag attaaaaaaa ccattgaagc gacggctcct
    32101 ggcagttttg aagttttcat tcacaacgac atggaagcgg caatgaaagt gaaatccggg
    32161 caactggatt actacatcgg tgcgtgtaat accggtgcgg gcgcggcatt gtcgattgcc
    32221 atcgcggtga ttggctataa caaaagttgc accattgcca aaccaggcat taaagcgaaa
    32281 gacgagcata tcgccaaaat gatcgctgaa ggaaaagtgg cgtttggcct ttccgttgag
    32341 cacgtcgaac acgcgattcc gatgctgatt aaccatctga aataaaaggc acgactatgg
    32401 atctgtatat tcagattatc gtggtggcgt gcctgacggg tatgacatcg cttctggcgc
    32461 atcgctcggc ggctgttttt catgacggca tccgcccgat cctgccgcaa ctgattgaag
    32521 gctatatgaa ccgtcgcgag gcggggagta tcgcttttgg tctgagcatt ggttttgtgg
    32581 cctcggtggg gatctctttt accctgaaaa ccgggctgct caacgcatgg ttactctttc
    32641 ttcctaccga tatcctcggc gtactggcga taaacagcct gatggcgttt ggtcttggcg
    32701 ctatctgggg cgtgttgatc cttacttgcc tgttgccagt aaaccagctg ctgaccgcgc
    32761 tgccggtgga tgtattaggt agcctcgggg aattaagctc gccggtagtt tctgcttttg
    32821 cactgttccc gctggtggcg attttctacc agtttggctg gaagcaaagt ctggtcgccg
    32881 ccgtggttgt tctgatgacc cgtgtggtag tcgtgcgcta tttcccacat cttaaccctg
    32941 aatccatcga aatctttatt ggcatggtga tgctgctggg gatcgcgata actcacgacc
    33001 tgcgtcatcg tgatgaaaat gatattgatg ccagcgggct ttcggtgttt gaagaacgca
    33061 cgtcgcggat tatcaaaaac ttaccctata tcgccatcgt gggagcattg attgccgccg
    33121 ttgccagcat gaagattttc gccggcagtg aagtgtcgat cttcactctg gagaaagcgt
    33181 actccgcagg cgtaacgccg gaacaatcgc aaacgctgat caaccaggcg gcactggcag
    33241 aatttatgcg cgggctgggt tttgtaccgt tgattgccac caccgcctta gcaacgggtg
    33301 tgtatgcagt tgcgggcttt acctttgttt atgcggtggg ctatctctcg ccgaatccga
    33361 tggttgcagc ggtattaggc gcagtggtta tttcggcgga agtcttgctg cttcgttcta
    33421 tcggcaaatg gctgggacgc tacccgtcgg tgcgtaatgc gtcggataac atccgtaacg
    33481 ccatgaatat gctgatggaa gtggcgctgc tggtcggttc gattttcgca gcaattaaga
    33541 tggcgggtta taccggattc tctatcgcgg ttgccattta cttcctcaac gaatccctgg
    33601 gccgtccggt acagaaaatg gcggcaccgg tcgtggcagt aatgatcacc ggtattctgc
    33661 tgaatgttct ttactggctt ggcctgttcg ttccggctta aggaaggcac ctatgaagac
    33721 gtttcctctg caaagcctga cgcttattga ggcgcagcaa aagcagtttg cgctggtgga
    33781 cacgatttgc cgtcactttc ccggcgcgga gtttctaacc agcggtgatt tgggcttaac
    33841 gccggggctg aatcaaccgc gtattaccca acgggtggag caggtgctgg ctgatgcatt
    33901 tcacgcacag gctgcggcgc tggtgcaggg cgcggggact ggcgcgattc gcgccgggct
    33961 ggcagctttg ctcaaaccgg ggcagcgtct tctggtgcat gacgcgcctg tttacccgac
    34021 gacacgggtt attattgagc agatggggct gacgcttatt actgttgatt tcaatgacct
    34081 gtcggcactg aagcaggtcg tcgacgagca acaaccggat gcggcgctgg tgcagcatac
    34141 gcgccagcag ccgcaggaca gctacgtgct ggcagatgtg ctggcaacgt tgcgcgcggc
    34201 aggtgttcca gcgttaaccg atgacaacta tgcggtgatg aaggtggcgc gaatcggctg
    34261 tgaatgcggc gcgaatgtct cgacattttc ctgcttcaag ctatttgggc cagagggtgt
    34321 tggtgcagtg gtcggcgatg ctgatgttat caaccgtatt cgcgccacgc tttactccgg
    34381 cggtagccag atccagggcg cacaggcgct ggaagtattg cgttgtctgg tgtttgcgcc
    34441 agtgatgcac gcggtgcagg caggggtatc tgaacggttg ctggctttgc ttaacggtgg
    34501 tgaggtgccg gaagtgaaaa gcgcggtgat tgctaatgcg cagtcgaagg tgttgattgt
    34561 cgagtttcat cagccgattg ccgccagagt gctggaagag gcgcaaaagc gcggtgcctt
    34621 gccttacccg gtgggtgcag agtcgaaata tgaaatcccg ccgctctttt atcgcctttc
    34681 cggaacgttt cgccaggcga atccacaatc agaacattgt gcgattcgca ttaacccgaa
    34741 tcgcagcggt gaagagacgg tgctgcggat tttgcgtgag agtattgcca gtatttaatg
    34801 ctggatagca ccgtcaagct aaattccgta ctgaacggtc ccctcgcccc tttggggaga
    34861 gggttagggt gaggggaacg tacgggatgt aaaattcaac gcgccagcat cgccggatgc
    34921 gatgctggcg catcttatcc ggcctacggt taatgtcttt tattatcaat agttaaggtg
    34981 atgcgattgg cgcggcagaa gagttcggag acgtgcaccg gtttgtcatt ctgatcaaag
    35041 gcgattttgc tgatgcgaaa caacggttcg cccagttcgc atttcagcca ttttgcctgc
    35101 gggcgggtgg cggcaaagat atcgatggtc tttttgtcgc tgaccactcg cgtgtcaaaa
    35161 cgctcctgaa ataactgata ggtggagctt ccttcgacgt aaatctcatc aaagtcagga
    35221 taacgcgaca gcgggatcca tgaactatca ataaacagcg gctctttatc gagatacatc
    35281 acccggcaga gatggaacac ttcgctgttt ccggggatgt tcagcttttt gcaaaacggc
    35341 gcggcgctga cccgttcctg ttcgatcact ttctctttcg tcgccttgcc ttgtgagacg
    35401 ccaaaatcgg taaaaccact gacagtaagc agggcgtttt caactttctg gctttgtaca
    35461 aaggtgcctt ttccctgcca gcggatcagt acgccgtccg ccactaagtc gctgatggct
    35521 ttgcgaatgg taatgcggct gacgttatat tgtgtacaaa gctcgttttc ggtagggatc
    35581 tgttgcccgg cctggtaaac cccctgcgcg atatcatcca gcagtcgctg gcggacggta
    35641 gcgtagagga gttgatgaga gtagcggtcc gtagctgaca taatcgaccc tgaaataaag
    35701 aatgaattac tcggattata gggtcgtttt ccggcaagta acagcacgtt ggcgtgaaga
    35761 tgctcacgcc aacgttatac ctaccaggca ccgtggtact gaatggtttt cgccgcgcac
    35821 gccgttccct gcgccatcgc ctgcggtaat gtcatccccg cagaccagcc gcaaaggaat
    35881 ccggcaatga acgaatctcc ggcacccatg gtgtcgataa ccgtcaccgg ttcaggagcc
    35941 tgacgccaga actgcgcgcc atcccaggca atgctgccgt tttcacccag cgtgacaatc
    36001 actgttcctg cgccacgggc aacaatcgct ttcatcttca gacgcagcgt ttcgtcttct
    36061 tgcggtgcgg aggcaaaggc aaaatcgaga tgcggcacca gtgtctgcca gagcgggctg
    36121 tcccacttgt cggagaagtc gaaagcggta agtttgcccg cagcgtgcag ctgtgggaat
    36181 gcgtcttccg catgtcccca gattgccgcg tgcacaatgt catactgcgc cagccaggcg
    36241 taatcctctt cactcagggc aaagtcggcc atcacgcctt cggtgtagtc gccaaaaacg
    36301 cgatcattgt cgtgcagttc cacctgagtt tgtgcggtaa cgccgtgttt cgtatggaca
    36361 tggctgatat cgacgcccat gcgggcgaga tcctgcttca gctttgtgcc gtagtcatcg
    36421 tcacccaccc aggtaatgca tcccggctgt atgccgtagc gagtgcagta caccgccaca
    36481 ttgaccgcat taccgccaga aaacgcttta ttcagttgcg ggtagatatc gacgcagtta
    36541 tcgccgattg tcgccagggt tttcatctct catcctccgg cagcagcgcg cgaaagcgtt
    36601 ccagcgcctg gcgggcatag agtctgggct cgttcatata catcgtcacc agctccaccg
    36661 tacagtaacc ctcatagccc cgctcaataa tatcgcgcat cagttcccgc agcggcattt
    36721 tgccttctcc aggaatgtaa tgcgtgtcgc tggccccgtc gctgtcgaca atatgcagat
    36781 gacgtaattt atcgcccagt ttgtcgaaat aactcatcac cggttccgcc tggacatacg
    36841 gcgcgcaaat gtcgaccatg ctgaacaggc gcggcgaagg caccagcgcc agcgcatgaa
    36901 gcacatcatt agcattacac acgatgttcg attcatacgg cgttaacggt tcgaggatta
    36961 gatccatgcc gatgttttcc gcgtactcat acagttcgct taggttctct gccagccgtc
    37021 cccagataac attaggtggc gtgagatagc ccgcgtgggc cgcggaaatc agcgtataac
    37081 ccgcgttcat ctcttttgcc atatccatcg ccagcttgat catgtcgagg ctttcgcgac
    37141 gcatatgttc atcgcccagc atcatgttat acggatagcc attggtttct ggcgtatagc
    37201 cgataatcgg catctgatac gtctgcgcca gcgccttgat ttgtttgatg ccgcccgctt
    37261 ttaagtccgg cgcgaacgcg tgcgggcgac cgccccaaat ttcgatgccg tcgtaaccca
    37321 gctcgcttgc atcacgaaat gcgtgttcaa taggcagccg ctggtggccg caggtaaaca
    37381 tacctgtttt catcgggtaa ctccttgcgt aaatccggcc cgttgccggg ccgggcgagg
    37441 gattaatatt ccaccagacc accgtaatag cggcgttcat ccgggttgtg atctttgtaa
    37501 atagacaggt agtagcagag ccactccatt ggcacgaaca tcaggaacgg tgccagccac
    37561 gggtgcagcc cttgcgaaat ttcggcgtaa tcgatgacga tcacgttgtc agtacgctgt
    37621 ttaacaaagt taatggcgcg ttcggtggtg tggcgacttt catcattgcc gagcaggaac
    37681 aggaacggaa cgcccggttc gacaatctcc agcgggccat ggcggaactc tccgctctca
    37741 atcacgcagc cgtgcgtcca ggtaaattcc atcagcgtta caatgccttc tttgtaaccc
    37801 agcggacgca gcggacccgc agcaacggta taaatcatcg gccactggct ggccagttca
    37861 ccaagctggc ggcctttttc ttcccaggtg cgtaccagat gaccgagcgc attcggcaac
    37921 tgcttgagat cgtttttgat cttgccgatt tccgcgttcg gcgcgaggcg ggtgatcatc
    37981 tccagcacca cgctgtagca gagcagcagg tgaatttccc agatacagtc ggcctgataa
    38041 tcaatgctaa attccgccgc cgaggtaatc gggctgtccg cgcgtttggt gaacgccgca
    38101 gtgagtgcgc cgcaggcccg gcccagctcc agcgctttga ttacctcttc cgttttaccg
    38161 tagtcagaaa cgccaatcac cgcgcaacga tcgtcgaggc gatacggggt gttatcgcag
    38221 aactcccagc cggaaatggc gtagacctga agatcggaaa agcgatccgc cagatgtttc
    38281 gccgcttgcg ccgcgttgag tggcgagccg caggcaacga aataaatacg atcaatgtcg
    38341 cgcttcacca tctcttccac gatggcgtgc accagcggaa cgtcatggct gagaactttt
    38401 tccacttcct gaaccatatt ttcggtgacc agaaagtcca cggtgctttt atcaatatcc
    38461 aacattttat tttctccaga taacattacg acaaagcgtt gagctggcgg ctacgcttcg
    38521 cccagaaggc gtaagcaggc agaccagtag caataacgat gacggcgcag ataaggccgg
    38581 gaatcggtgc ccagacaaag gttgaggcga ccagaatgag gcttgacgca atggcgaggg
    38641 tggtcatcag cccgaaagca ggagtacgcc acagcggttt gtaatcgtcg cgtttacgac
    38701 accagatgat ggagccgaag gtgagggtat ttttgaaaca catcaccagg gtgaaataac
    38761 ccagcaggct ggtgagatcg gaaacgaaga tgaagaagag ccccagcgcc ccttgcagga
    38821 tgatggagac atccggcgtg ttgtatttcg gatgcacatg gccgaagcat ttaaagaaca
    38881 ggttgtcttt cgccatcgcg tattccagcc gcggctggta catcacgcag ctggaaagcg
    38941 aaccaagaat gacgatcatc gccgtgatgg caacaaagat cccagcggtg ctgccgagtg
    39001 cggggatcca ggtcagggcg tcggaaatcg gcgtttcaga attggcgagt ttgtcgaagg
    39061 gcatcaggtc ggaaatcacc agcgccagca gggtgtagag caccagaacc agcagacagg
    39121 aaccaatcag cgctcgtggc atggtttttc cggggttttt aatttcgccg gtcatataac
    39181 agatagaggc catgccggta tacgaccaac tggtggcaga gatccccgcc agcagcgcca
    39241 taaagctgcc cgttgcgcca atcgcagtgg tggtaggggc ggcaaaattc tccgctttaa
    39301 accagaagat cccaaggcca atgacgatag tgaacgggat aattttggcg atggtaatta
    39361 gcgtctgaaa cgctgcgccg ccttcaacgg agcgcaggtg tagcaacata aaggcgataa
    39421 ttaatccggc ggcgataaat ttaccgggca acggatcgat aggcgttaaa aagccaagat
    39481 tgctgacaat cgccagcgcc ataatcgaca atgacggcgc atcgttggcc cagaagctgg
    39541 cccagccgga gaggaaagcc agcggtcggc ttccggcatt tttcagataa acataatctg
    39601 cgccattttc cggataagcg gtggatagtt ccgcatagac gcacatttgc gggatcacaa
    39661 ttaacccgcc aatgacaaac gtgagcaccg taagccacgg cgtgcccgct gcttttgcca
    39721 cttcacccac agatacaaaa ataccggagc cgacggttgt cccgacggcg attgcaagaa
    39781 cggcccaaaa tccgagcttg cgttggagtt cctggcttcc catagcgata cctttttctt
    39841 gtccaacacg ttgtagggga gagtttgcag tcatttaaaa ggtaacatga caatgcatga
    39901 tgaatataat aacatatgat gttatgtaat gcggaggaag atcacaaaat ttcgcacagg
    39961 atcgcgctgt ggctaatgaa tgtagttatc caattgaaat taaagtgaaa atatttttac
    40021 ggggggggag caagaaggat atagaaacaa atacgccctc ggaaaatcca gagggcgtcg
    40081 ggcaattaaa ccggtgttag ccgatttctg tcagagactt actgtgcagt aggaccgcaa
    40141 ccgccaatga ttttagaaat agaaattgcc gggtgcagca ggtagtcgta agagcagttc
    40201 ttatcacggt tttcaatatg accagtacat gcagttaacg tagccagtac tgcacctacc
    40261 agtgcaactt taataaattt gttcatttac att
//
LOCUS       DAJVTY010000013        40176 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVTY010000013 DAJVTY010000000
VERSION     DAJVTY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40176
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.013"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(350..556)
                     /locus_tag="OQQ66_000670"
     CDS             complement(350..556)
                     /locus_tag="OQQ66_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1903706.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            869..958
                     /gene="kdpF"
                     /locus_tag="OQQ66_000671"
     CDS             869..958
                     /gene="kdpF"
                     /locus_tag="OQQ66_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1903707.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            958..2631
                     /gene="kdpA"
                     /locus_tag="OQQ66_000672"
     CDS             958..2631
                     /gene="kdpA"
                     /locus_tag="OQQ66_000672"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1903708.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2654..4702
                     /gene="kdpB"
                     /locus_tag="OQQ66_000673"
     CDS             2654..4702
                     /gene="kdpB"
                     /locus_tag="OQQ66_000673"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS1903709.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4711..5254
                     /gene="kdpC"
                     /locus_tag="OQQ66_000674"
                     /pseudo
     CDS             4711..5254
                     /gene="kdpC"
                     /locus_tag="OQQ66_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5247..7931
                     /gene="kdpD"
                     /locus_tag="OQQ66_000675"
     CDS             5247..7931
                     /gene="kdpD"
                     /locus_tag="OQQ66_000675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS1903710.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7928..8605
                     /gene="kdpE"
                     /locus_tag="OQQ66_000676"
     CDS             7928..8605
                     /gene="kdpE"
                     /locus_tag="OQQ66_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1903711.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8821..9078)
                     /locus_tag="OQQ66_000677"
     CDS             complement(8821..9078)
                     /locus_tag="OQQ66_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903712.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9201..11399
                     /gene="speF"
                     /locus_tag="OQQ66_000678"
     CDS             9201..11399
                     /gene="speF"
                     /locus_tag="OQQ66_000678"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS1903713.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKISHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGFDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11396..12734
                     /gene="potE"
                     /locus_tag="OQQ66_000679"
                     /pseudo
     CDS             11396..12734
                     /gene="potE"
                     /locus_tag="OQQ66_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
     gene            12810..12959
                     /locus_tag="OQQ66_000680"
                     /pseudo
     CDS             12810..12959
                     /locus_tag="OQQ66_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            13083..13733
                     /locus_tag="OQQ66_000681"
     CDS             13083..13733
                     /locus_tag="OQQ66_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCS1903714.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(13774..14268)
                     /locus_tag="OQQ66_000682"
     CDS             complement(13774..14268)
                     /locus_tag="OQQ66_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903715.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(14483..16123)
                     /gene="pgm"
                     /locus_tag="OQQ66_000683"
     CDS             complement(14483..16123)
                     /gene="pgm"
                     /locus_tag="OQQ66_000683"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1903716.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(16149..16694)
                     /gene="seqA"
                     /locus_tag="OQQ66_000684"
     CDS             complement(16149..16694)
                     /gene="seqA"
                     /locus_tag="OQQ66_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1903717.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIAEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            16879..17643
                     /gene="ybfF"
                     /locus_tag="OQQ66_000685"
     CDS             16879..17643
                     /gene="ybfF"
                     /locus_tag="OQQ66_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1903718.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            17783..18076
                     /gene="ybfE"
                     /locus_tag="OQQ66_000686"
     CDS             17783..18076
                     /gene="ybfE"
                     /locus_tag="OQQ66_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1903719.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            18216..18746
                     /gene="fldA"
                     /locus_tag="OQQ66_000687"
     CDS             18216..18746
                     /gene="fldA"
                     /locus_tag="OQQ66_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1903720.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            18956..19042
                     /gene="uof"
                     /locus_tag="OQQ66_000688"
     CDS             18956..19042
                     /gene="uof"
                     /locus_tag="OQQ66_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1903721.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            19035..19481
                     /gene="fur"
                     /locus_tag="OQQ66_000689"
     CDS             19035..19481
                     /gene="fur"
                     /locus_tag="OQQ66_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1903722.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(19566..19892)
                     /locus_tag="OQQ66_000690"
     CDS             complement(19566..19892)
                     /locus_tag="OQQ66_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903723.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(19942..21348)
                     /gene="chiP"
                     /locus_tag="OQQ66_000691"
     CDS             complement(19942..21348)
                     /gene="chiP"
                     /locus_tag="OQQ66_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1903724.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLLGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(21795..23459)
                     /gene="glnS"
                     /locus_tag="OQQ66_000692"
     CDS             complement(21795..23459)
                     /gene="glnS"
                     /locus_tag="OQQ66_000692"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1903725.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(23662..25608)
                     /gene="nagE"
                     /locus_tag="OQQ66_000693"
     CDS             complement(23662..25608)
                     /gene="nagE"
                     /locus_tag="OQQ66_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1903726.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            25941..26741
                     /gene="nagB"
                     /locus_tag="OQQ66_000694"
     CDS             25941..26741
                     /gene="nagB"
                     /locus_tag="OQQ66_000694"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1903727.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26801..27949
                     /gene="nagA"
                     /locus_tag="OQQ66_000695"
     CDS             26801..27949
                     /gene="nagA"
                     /locus_tag="OQQ66_000695"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1903728.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            27958..29178
                     /gene="nagC"
                     /locus_tag="OQQ66_000696"
                     /gene_synonym="nagR"
     CDS             27958..29178
                     /gene="nagC"
                     /locus_tag="OQQ66_000696"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1903729.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            29226..29978
                     /gene="nagD"
                     /locus_tag="OQQ66_000697"
     CDS             29226..29978
                     /gene="nagD"
                     /locus_tag="OQQ66_000697"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1903730.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            30375..32039
                     /gene="asnB"
                     /locus_tag="OQQ66_000698"
     CDS             30375..32039
                     /gene="asnB"
                     /locus_tag="OQQ66_000698"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1903731.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            32420..32496
                     /locus_tag="OQQ66_000699"
     tRNA            32420..32496
                     /locus_tag="OQQ66_000699"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32454..32456,aa:Met,seq:cat)
     gene            32505..32589
                     /locus_tag="OQQ66_000700"
     tRNA            32505..32589
                     /locus_tag="OQQ66_000700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32539..32541,aa:Leu,seq:tag)
     gene            32613..32687
                     /locus_tag="OQQ66_000701"
     tRNA            32613..32687
                     /locus_tag="OQQ66_000701"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32645..32647,aa:Gln,seq:ttg)
     gene            32722..32796
                     /locus_tag="OQQ66_000702"
     tRNA            32722..32796
                     /locus_tag="OQQ66_000702"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32754..32756,aa:Gln,seq:ttg)
     gene            32812..32888
                     /locus_tag="OQQ66_000703"
     tRNA            32812..32888
                     /locus_tag="OQQ66_000703"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32846..32848,aa:Met,seq:cat)
     gene            32937..33011
                     /locus_tag="OQQ66_000704"
     tRNA            32937..33011
                     /locus_tag="OQQ66_000704"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32969..32971,aa:Gln,seq:ctg)
     gene            33049..33123
                     /locus_tag="OQQ66_000705"
     tRNA            33049..33123
                     /locus_tag="OQQ66_000705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33081..33083,aa:Gln,seq:ctg)
     gene            complement(33277..34452)
                     /gene="ubiF"
                     /locus_tag="OQQ66_000706"
     CDS             complement(33277..34452)
                     /gene="ubiF"
                     /locus_tag="OQQ66_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1903732.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMVDNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            34598..36022
                     /gene="miaB"
                     /locus_tag="OQQ66_000707"
     CDS             34598..36022
                     /gene="miaB"
                     /locus_tag="OQQ66_000707"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1903733.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            36175..37215
                     /locus_tag="OQQ66_000708"
     CDS             36175..37215
                     /locus_tag="OQQ66_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1903734.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            37212..37679
                     /gene="ybeY"
                     /locus_tag="OQQ66_000709"
     CDS             37212..37679
                     /gene="ybeY"
                     /locus_tag="OQQ66_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1903735.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            37769..38647
                     /gene="corC"
                     /locus_tag="OQQ66_000710"
     CDS             37769..38647
                     /gene="corC"
                     /locus_tag="OQQ66_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1903736.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            38672..>40176
                     /gene="lnt"
                     /locus_tag="OQQ66_000711"
     CDS             38672..>40176
                     /gene="lnt"
                     /locus_tag="OQQ66_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1903737.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAA"
BASE COUNT         9569 a        10290 c        10456 g         9861 t
ORIGIN      
        1 gaacaggcca cgccggtggg ggcaccaatg cgcaccccgg ctgaaccctg aacaatgcta
       61 ccgccatact gcgtcatgtc gccctgacgc gctgccggtt ttccgctcat ttctcatcct
      121 tgttttatcg ggttatttgt ccgctgacag gcaaaaccct acagatttat aagaaagcct
      181 taagtgagat atccatcaga tagaaacaat taggaaaata tttatatatt tttcctgaat
      241 atatttcagc tgggtaatac catatctaaa tatttacctg aacatcttcc taataaaaaa
      301 accgccagca tctccactag cggttttcaa tgtattcaaa actcggcaat cagtaaaaat
      361 caccggttgc cttttcggcc tgttccatcc aaaccggttt atcgctggtt ttcgaccaga
      421 cgcggtgcag gtagctgtaa aagcgggcgc ggtctttcca gaaaagcatg aaaggcagcg
      481 ccagaacgcc cgctgcaacc gcatatgtac ggcgtaaaaa gataagccat gcaggatatt
      541 ctctgtagag ttccatactt ttctccccct tttgtatcta cccggtgaat agcaccggaa
      601 aaatgaattt gtttatctga tgaaaatagt accgcctttt gtgtaatttt actactcatc
      661 cgaccactta tttttgctta ttgatggttt atttacattc actctgtaat taagttgcac
      721 aaaagttaaa ttaatgctaa acattagtta aaccatggct tttgccattt ttatactttt
      781 tttacacccc gcccgcagat ttttgcgaaa tctttgcagc cagaattcta ctcttccggt
      841 atcactttta ggccactgga ggtgcactgt gagtgcaggc gtgatagccg gcgtgttgct
      901 ggtgttttta ttactgggtt atttggttta tgccctgatc aatgcggagg cgttctgatg
      961 gctgcgcaag ggttcttact gattgccacg tttttactgg tattaatggt gctggcgcgt
     1021 cctttaggca gcgggctggc gcggctgatt aatgacattc ctcttccagg tacagcgggc
     1081 gttgaacgta tactctttcg tttacctggc gtctctgacc atgagatgaa ctggaagcaa
     1141 tatctttgtg ccattctcgg cctgaacatg ctggggctgg cggtgctgtt ttttatgttg
     1201 ctcggtcagc actatctgcc gcttaatcca cagcagttgc cggggctgtc gtgggatctg
     1261 gcgctgaata ccgccgtcag ctttgtcacg aataccaact ggcaatctta tagcggtgaa
     1321 accacgttga gctatttcag ccagatggca ggcttaacgg tacaaaactt tctttctgcc
     1381 gccagcggga ttgcggtgat ttttgccttc atccgtgcgt ttactcgcca gagcatgagc
     1441 acactcggga atgcctgggt cgatctgcta cgcatcacgt tatgggtgct agtccctgtg
     1501 gcgttgttga tcgcactgtt ttttattcaa caaggtgcgc tgcaaaactt tctgccctat
     1561 caggcagtga ataccgttga aggagcgcaa cagctgttac ccatgggacc agtagcttct
     1621 caggaagcga tcaagatgct cggtactaac ggcggtggct tctttaatgc caactcgtcg
     1681 catccgtttg aaaacccaac cgcactgacc aacttcgtgc agatgctggc gatcttcttg
     1741 atcccaacag ctctgtgctt tgcctttggt gaagtgacgg gcgatcgccg ccaggggcgc
     1801 atgttgctgt gggcgatgtc agtgattttt gtcatctgcg taggcgtggt tatgtgggca
     1861 gaagttcagg gtaatcctca tctgctggcg ctcggcgcag acagcagcat caatatggaa
     1921 ggtaaagaga gccgtttcgg cgtgctggtc agtagcctgt ttgcggtcgt gactacggcg
     1981 gcgtcctgtg gcgcggtgat tgcgatgcat gattcgttta ccgctctcgg cggcatggtg
     2041 ccgatgtggc taatgcaaat tggtgaagtg gtgttcggcg gtgtcggttc tggtctttac
     2101 ggcatgatgc tgtttgtcct gctggcggtg tttattgccg ggctgatgat tggtcgtaca
     2161 ccggaatatc tgggtaaaaa aatcgacgta cgcgagatga aactgactgc actggcgatt
     2221 ctggttactc caacactggt actgatgggc gcggcgttgg cgatgatgac cgacgccgga
     2281 cgtagcgcca tgcttaaccc tggcccgcat ggttttagcg aagtgctgta cgccgtgtcg
     2341 tccgccgcta acaacaacgg cagcgccttt gccggattaa gcgccaactc tccgttctgg
     2401 aactgtttac tggcgttctg catgtttgtc ggtcgcttcg gggtgattat cccggtgatg
     2461 gcaattgccg gttcgctggt gagtaaaaag agccaacccg ccagctccgg cacgctgcca
     2521 acgcacggcc cgctgtttgt tggcctgtta atcggcaccg tgttgctggt tggcgcactg
     2581 acctttatcc ctgccctggc gcttggtccg gtggcggaat atctctcctg atgatattga
     2641 gtgagcactg aatatgagtc gtaaacaact ggcgctattc gaaccaacac ttgtcgttca
     2701 ggcgctgaaa gaagcggtga aaaaattaaa cccgcaggcg caatggcgca atccggtgat
     2761 gtttatcgtc tggatcggca gtctgctgac cacctgtatt agcatcgcga tggcaagcgg
     2821 tgcgatgcct ggcaatgcgc tgtttagcgc ggcaattagc ggctggctgt gggtcaccgt
     2881 actgttcgct aatttcgccg aggcgctggc agaaggccgc agtaaagcgc aggccaacag
     2941 tcttaaaggg gtgaaaaaaa ctgcctttgc ccgcaagctg cgtgagccga aatatggcgc
     3001 tgcggcggac aaagttcctg ccgatcaact gcgtaaaggt gacatcgtgc tggtagaagc
     3061 tggcgatatt atcccctgcg atggtgaagt cattgaaggc ggcgcatcgg tcgatgaaag
     3121 cgccatcacc ggggaatcgg caccggtgat ccgtgaatcc ggcggcgatt ttgcctccgt
     3181 caccggcggc acgcgtattc tttctgactg gctggtgatt gagtgtagcg ttaaccccgg
     3241 tgagacattt ctggatcgga tgatcgcgat ggtggaaggc gcacagcgac gcaaaacacc
     3301 gaacgagatt gccctgacta tcctgctgat tgctctgacc atcgtctttt tactggcaac
     3361 cgccacgctg tggccgtttt ccgcgtgggg cggtaatgcc gtgagtgtaa cggtactggt
     3421 ggcgctgctg gtctgtctga tcccaaccac tattggcggc ctgttgtcag cgatcggcgt
     3481 cgccgggatg agtcggatgc tgggcgcgaa tgtgattgcc accagcggac gtgcagttga
     3541 agcggcaggt gacgttgacg ttctgctact ggataaaacc ggcaccatca cactcggtaa
     3601 ccgtcaggcg tcggagttta tccctgcgca gggcgtggat gaaaaaacgc tggctgacgc
     3661 cgcacaactg gcttcgctgg ctgatgaaac gccggaaggc cgcagtattg tgatcctcgc
     3721 caagcaacgt tttaacctgc gcgagcgcga tgtgcagtcg ctccatgcca cctttgtacc
     3781 gtttactgcg caaagccgga tgagcgggat caacatcgac aaccgcatga tccgtaaagg
     3841 ttctgtcgat gccattcgcc gccatgttga ggctaacggc ggtcacttcc ctgccgatgt
     3901 tgatcaaaaa gtcgatcagg ttgcgcgtca gggggccacg ccgctggtgg tggtggaagg
     3961 ttcccgtgtg ctgggcgtta ttgcgctgaa agatatcgtc aaaggcggta ttaaagagcg
     4021 cttcgcccag ctgcgcaaaa tgggcattaa aacggtgatg attaccggcg ataaccgtct
     4081 gactgccgcc gccattgctg cggaagcggg cgtcgatgat tttctcgccg aagcgacacc
     4141 ggaggccaag ctggcattga ttcgtcagta tcaggcggaa ggccgtttgg tggcgatgac
     4201 cggcgacggc accaacgatg ctccggcgct ggcgcaagca gatatcgcgg tggcgatgaa
     4261 ctccggcacc caggcggcga aagaggcggg caatatggtc gatctcgact ctaacccgac
     4321 caagttgatc gaggtggtgc atattggcaa acagatgctg atgacccgtg gctcgctgac
     4381 caccttcagc attaccaacg atgtggcgaa atacttcgcc attattccgg cggcgttcgc
     4441 ggcaacgtat ccgcagttaa atgcgctgaa catcatgcgc ctgcattcgc ccgactccgc
     4501 cattctcagt gcagtcattt tcaacgcctt gattatcgtc tttttgattc ctctggcgtt
     4561 aaaaggcgtg agttataaac cgcttaccgc ttctgccatg ttgcgccgta acttatggat
     4621 ttacggtctg ggtgggctgc tggtgccgtt tattggtatc aaagtcattg atttactgct
     4681 gaccgtttgc ggtctggtgt gaggtttacc atgcgtggct tacgtccggc attatcgaca
     4741 tttatctttc tgttattgat tactggcggc gtttacccgc tgctgaccac cgtgctgggg
     4801 caatggtggt ttccctggca ggccaacggt tcgttgattc gtgaaggtga tacggtgcgg
     4861 ggttcggcat taatcgggca gaattttacc ggcaacggca gaaatgccct ataatccaca
     4921 ggcttctggc gggagcaatc tggcggtcag taaccctgag ctggataaac aaatagccgc
     4981 acgcgttgct gcattacggg ccgctaaccc ggatgccagt acgaacgttc cggttgaact
     5041 ggtgacggca tcggcaagcg ggctggacaa taatatcacc ccgcaagcgg cggcctggca
     5101 gatcccacgc atcgcgaaag cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc
     5161 aaaatacagc caacaaccgc tggtgaaata tatcggccag ccggtcgtca acattgtcga
     5221 actcaatctg gcgctggata agcttgatga ataacgaacc cttacgtccc gaccccgatc
     5281 gtctgctgga acaaactgcc gcgccgcatc gggggaagct gaaagttttc ttcggtgcct
     5341 gtgcaggcgt cgggaagacc tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag
     5401 ggctggatat tgtggttggc gtggtagaaa cccacgggcg aaaagatacc gacgcaatgc
     5461 tggaagggct ggctgttctg ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg
     5521 agtttgacct cgatgccgcc ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg
     5581 cgcacagtaa tgcgctaggt tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc
     5641 tggaagcagg cattgatgtt ttcactaccg tcaacgttca gcatctggaa agtctgaatg
     5701 atgtggtcag cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat ccttttttcg
     5761 atgccgccga cgacgtggtt ctggtggact tgcccccgga cgatctgcgc cagcggctga
     5821 aagaaggcaa agtctatatt gccgggcagg cggagcgcgc cattgaacat tttttccgca
     5881 aaggcaatct gatcgccctg cgcgaactgg cactgcgccg tactgccgat cgcgttgatg
     5941 agcaaatgcg cgcctggcgg gggcatcctg gcgaagagaa agtctggcac acgcgcgacg
     6001 cgatcctttt atgcatcggc cataacaccg gtagtgagaa gctggtccgc gcggcggcgc
     6061 ggctggcgtc acggctgggt agcgtctggc acgcggtgta tgttgaaacc cctgccctgc
     6121 accgcttacc ggaaaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc
     6181 tgggcgcgga gacggcaaca ctttctgatc cagcggaaga aaaagcagta gtgcgttatg
     6241 cccgtgaaca taatctcggc aagattattc tcggtcgccc ggcctcgcgc cgctggtggc
     6301 gtcgggaaac gtttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg
     6361 tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctctttta
     6421 aagacaagtg gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta
     6481 tcactttaat tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt
     6541 atctgcttgg cgtggtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcta
     6601 ccgtcattaa tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg
     6661 tctctgatgt gcaatatctg ctgaccttcg cagtgatgtt aaccgtcggg ctggtgatcg
     6721 ggaaccttac tgctggcgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca
     6781 cacggcactt atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg
     6841 ccgccaccat cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc
     6901 ccgatgacaa cggtaaattg cagccgttaa cacatccgca aggaatgacg ccatgggacg
     6961 ataccatcgc gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt
     7021 tacccggtgt accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg
     7081 tggtagtgga gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg
     7141 agacgtttac gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag
     7201 aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa cgccctactg gcagcgcttt
     7261 cgcatgattt acgcacgccg ctcacggtgc tgttcggtca ggcagaaatc ttaacgctcg
     7321 atctggcaag cgaaggatcg ccccacgccc gccaggccag cgagattcgt cagcatgtgc
     7381 tgaacactac ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct
     7441 ttaatttgaa gaaagagtgg ttaacgctgg aagaagtggt cggtagcgcg ctgcaaatgc
     7501 tggaaccggg tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc
     7561 acgttgacgg gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat
     7621 atgcgggtgc gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac
     7681 tggatgtctg ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata
     7741 agtttgctcg cgggaataaa gagtcggcag taccgggggt agggcttgga ctggcaattt
     7801 gtcgggcgat agtggatgta cacgggggca ctattaccgc gttcaaccga ccggaaggtg
     7861 gtgcctgttt tcgtgttaca cttccccagc aaactgcccc tgaacttgaa gaatttcatg
     7921 aggatatgtg acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg
     7981 cacggcgctg gagggcgacg ggatgcgcgt ctatgaggcc gaaacgctgc aacgcggctt
     8041 gctggaagcg gctacccgta agccagattt gattattctc gatctcggcc tgcccgatgg
     8101 tgatgggatt gagtttatcc gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct
     8161 ttccgcacgc agcgaagaga gcgacaaaat cgccgcgctg gatgccggag cggatgatta
     8221 tctgagtaag ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg
     8281 ccactccgct accgccgcgc ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt
     8341 agccgcccgc gtgattcacc ggggtgagga agaggtgcat ctcacaccaa ttgagttcca
     8401 cctgctggcg gtgctgctca acaatgccgg aaaagtactc acccagcgcc agctccttaa
     8461 ccaggtgtgg gggccaaacg cggtcgaaca cagtcactat ttgcgtattt atatgggaca
     8521 tctgcgacaa aaactggaac aggatcccgc ccgcccacgc catttcatta ctgaaaccgg
     8581 tattggctat cggtttatgc tttgaatatt aattttaata cagcctgcct tttattaatt
     8641 aaagccgtaa taataaatac ggctttttat cttaaacaac acacaaaaat aacaattcaa
     8701 tattttatat tactgagtaa aaagctcatc attaaataaa ttaagattga tcatttttta
     8761 ttgatcacct tcacagttca accgtatttc ctggatagaa tatcttcacc ttcattcaca
     8821 tcaggaaagg taaattaaat ggaaaataac agccgcacta tgccccatat aaggcggaca
     8881 actcatatta tgaagtttgc tcatcgcaat agcttcgact ttcacttctt caatgcccgt
     8941 tagtctaccg actaagggca cttcagcgta caggtcttcc tgactctctg tattacaggc
     9001 atatgcctgt attccgcagt gctcattctc tgttaaccct tgtattatca gccaccggac
     9061 tggtaagggt ttctgcattc tgaaaaaagc aatctacctg atttatttat cagcggggta
     9121 actttgcttt tttccggcac gatcgatttc tcattcgaga aattgaggac ctgctattac
     9181 ctaaaataaa gagatgaaaa atgtcaaaat taaaaattgc ggttagtgat tcttgcccgg
     9241 actgttttac cacgcagcga gaatgtatct acattaatga aagtcgtaat atcgatgtgg
     9301 cggcaatcgt tttatcgctc aacgatgtta catgcggaaa actcgatgaa atcgatgcca
     9361 cgggttatgg catcccggta tttattgcta ctgaaaatca agaacgtgta cccgcagagt
     9421 atttgccctg tatttcgggt gtctttgaga attgcgaatc gcgacgagaa ttttatggtc
     9481 gtcagttaga aaccgctgcc agccattatg aaactcaact gcgcccacct ttcttccgcg
     9541 cactggtcga ttatgtcaat caaggtaaca gcgcgtttga ttgccctggt catcagggcg
     9601 gcgaattttt ccgtcgccat ccggcgggga atcagtttgt ggaatacttt ggtgaggcgc
     9661 tgttccgttc cgacttgtgc aacgcagacg tagcaatggg tgatctgctg attcacgaag
     9721 gcgcgccatg cattgcacag caacatgcgg caaaagtgtt caatgccgat aaaacatact
     9781 tcgttctaaa tggcacttca tcttctaaca aagtggtttt aaacgccctg ctgacaccgg
     9841 gtgatctggt gctgtttgac cgcaataacc acaaatctaa ccaccacgga gcgttgctac
     9901 aggctggtgc aacaccggtt tatctggaaa ccgcacgtaa cccgtatggc tttatcggtg
     9961 gcattgatgc gcactgtttt gaagaaagtt acctgcgtga gctgatcgcg gaagtcgcac
    10021 cgcagcggac aaaagaggct cgtcctttcc gcctcgccgt tattcagcta ggcacctacg
    10081 acggtacgat ttataacgcc cgccaggtgg tggataaaat tagtcatctg tgtgactaca
    10141 ttctgtttga ctccgcatgg gtcggctatg agcaatttat cccgatgatg gctgactgtt
    10201 caccgctgtt gctggatctt aatgagaacg atccgggcat tctggttacg caatctgtgc
    10261 ataaacagca ggctggtttt tctcaaactt cacaaatcca taaaaaagac agccacatca
    10321 aagggcaaca gcgttatgta ccgcacaaac gcatgaacaa cgcctttatg atgcatgcct
    10381 ccaccagccc gttctatccg ctgtttgccg cactggatat caacgccaaa atgcatgaag
    10441 gtgtcagcgg tcgtaatatg tggatggatt gtgtggtaaa tggcattaat gcccgcaagc
    10501 tgatcctcga taactgtcag catattcgtc cgttcgtacc tgaactagtg gatggtaaac
    10561 cctggcagtc gtatgaaaca gcgcaaattg cggttgatct gcgcttcttc cagtttgtac
    10621 caggggaaca ctggcactct tttgaaggct atgcagagaa tcaatacttt gtcgatccat
    10681 gcaaactgtt gctgacaacc ccaggttttg atgcacgtaa cggcgaatat gaagcgttcg
    10741 gtgtacccgc gacgattctt gctaacttcc tgcgcgaaaa tggcgtagtg ccggaaaaat
    10801 gcgatcttaa ctccatcctc ttcctgctga ctccggcaga agatatggcc aaacttcagc
    10861 aacttgttgc cctgctggta cgcttcgaaa aactgcttga gtccgacgcg ccattagcag
    10921 aagtgctgcc ttccatctac aaacagcatg aagagcgcta cgccggttat acccttcgtc
    10981 agttatgcca ggaaatgcat gatttgtatg cccgtcacaa cgtgaaacaa ccgcaaaaag
    11041 agatgttccg taaggaacac ttcccacgcg taagcatgaa tccacaagaa gccaactacg
    11101 cctattcacg cggtgaagtg gagctggttc gtctgccgga tgccgaaggc cgtatcgctg
    11161 ccgaaggtgc gcttccttat cctccgggtg tgctgtgtgt tgttccgggt gaaatctggg
    11221 gtggtgctgt tttgcgttac ttcagcgctc tggaagaagg gatcaacctg ctgccaggtt
    11281 ttgcaccgga gctgcagggt gtctatatcg aagaacatga tggtcgtaag caagtttggt
    11341 gctatgtcat caagcctcgt gatgcgcaaa gcaccctgtt gaaaggggaa aaattatgag
    11401 tcaggctaaa tcgaacaaaa tgggcgtcgt tcagttaacc atactgacga tggtcaacat
    11461 gatgggctcc ggtatcatca tgctgccgac aaagcttgcc gaagttggga caatctcaat
    11521 tatctcctgg ctggtgacag ccgtaggctc aatggcactg gcatgggcat tcgcaaaatg
    11581 cggtatgttc agccgtaaat caggcggtat gggcggttat gcggagtatg ctttcggtaa
    11641 gtccggcaat tttatggcga actacaccta cggtgtctcg ctgctgattg ctaacgtcgc
    11701 gattgctatt tcggcggttg gttacggcac cgaactgctc ggcgcgagtt tgtcgccagt
    11761 gcagattggt cttgcgacca tcggagtgct gtggatttgt accgtggcta actttggtgg
    11821 tgcgcgcatt accgggcaaa tcagtagcat tactgtgtgg ggagtcatta ttccggtcgt
    11881 cggtctgtgc attattggct ggttctggtt tagtccgacg ctgtacgcta actcatggaa
    11941 cccgcaccat gtgccgttct ttagcgctgt aggttcttcc atcgccatga cgttgtgggc
    12001 tttcctcggt ctggagtcag cctgtgccaa cgccgaagta gtggaaaacc cggaacgtaa
    12061 cgtgccgatt gcagttcttg gcggtacttt aggtgcggcg gtgatttata tcgtctccac
    12121 caacgtgatt gccgggattg tgcctaatat ggagctggca aattcaacgg caccatttgg
    12181 cctggctttc gcacagatgt tcacaccaga agtgggtaaa gtcattatgg cgctgatggt
    12241 gatgtcctgc tgcggttcac tgcttggctg gcggttcact gcttggctgg cagttcacca
    12301 ttgcccaggt gtttaaatct tcatctgatg aaggctactt ccctaaaatt ttctcccgtg
    12361 taaccaaagt ggatgcaccg gtgcagggaa tgttgaccat tgtgattatt cagagtggat
    12421 tggcactgat gaccattagc ccgtcactga acagccagtt caacgtgctg gttaacctgg
    12481 ccgtggtgac caatatcatt ccgtatattc tgtcaatggc ggcattagtc attattcaga
    12541 aggtcgctaa tgtgcccccc tcaaaagcga aagtagcaaa ctttgttgcc tttgttggtg
    12601 cgatgtacag tttctatgcg ctgtactcat ccggggaaga agccatgctg tacggttcta
    12661 tcgtgacctt ccttggttgg acactgtatg gtctggtatc accatgcttt gaactgaaaa
    12721 ataaacacgg ttaataaaac aaagtaaaaa agggcgattg aatgatcgcc ctttctgttt
    12781 gttggcaaat agttcgttat ttatgccgca tccggcagat gtagggtcta aacgcgatcg
    12841 aacacagcaa ctatttgcgt aattatatgg ggcatctgcg acaaaaactg gaacaggatc
    12901 cggcccggcc tcgccatttt attactgaaa ccggtattgg gtatcggttt atgctttgaa
    12961 aaaagtggca atgtatggat aaagtcattt tttattcaaa tataaggaac ttaatattta
    13021 aaaaatgttc catacaattc ctcatcataa atgacaccgt agcacacaag gagcaagtag
    13081 cgatgcgtga aatgaatcag ttagtaaata ctgaagattc agcctggcca ataatccaga
    13141 actggttaaa agacgccaca aaccataccg aactcttacc ggttaataaa aatttagccg
    13201 aaaccgcact ttatcaatta caggtgacaa caaaatcacc gatgggtgcg cttgtctatg
    13261 gctccggagg tctgctgatt gataatggtt ggttacgcat agcaggatct ggtcaccccc
    13321 ggttgccacg agatccagta agttggacac aacgccctga attcgcaggc gtgcgggctt
    13381 tacccatcgc tgacgatgtc gcagggggaa tttttgctct taatggtggc gatcttgggg
    13441 aagatactgg ctgcgtttat tattttgcgc cagatacgct gaattgggaa agtctggaag
    13501 ttggatattc tgagtttctt cagtgggcat tatccggtga tttagatacc ttctatgaaa
    13561 atgtacgatg gcagcaatgg cgagaagacg taataaaatt atcggcaacg gaagccttca
    13621 cgttctaccc tttcttatgg gtacaaagtg aagaagcaag aacaagaaaa gttatttcgc
    13681 ttactgaact ctgggaaatg cagtatcaaa tgaaagaaac attcacgcaa taagacatgt
    13741 tcaggtccgg gtttcattcc cggactcact attttaatgt gtatttatcg ctttcaattc
    13801 ttgctggcaa ttttctgact ggcagcgtcg taactcaata atcccgacat gcatcatcga
    13861 gccaaaaaat ccgctttcgc tgacataatc ctcattgact atcggtcgag tgccgggctt
    13921 gccctggcaa acccgcataa aaaatgaggt gtagcattgc caaccgggat ttaaaccgac
    13981 cttttcttgt gctgacatat tatgaagata agtaagttct ttttcgttcc cgcctgacaa
    14041 ctgccagcgg cgggtattat caggtaagtt atcaccacgg caatttgtct tacaccggga
    14101 aggtgcaata tttaccgcaa tgctacccgt taatcttctg gttttatctt taggggtcag
    14161 taaccagtcg ccagctaatg atgattgttc aggttgggcg catgcagtca gcagtaatgt
    14221 ggcggtaatt agtgacacta tgacgaccag actcctgttg gtcttcattg ggatttcctt
    14281 gagctactca gtttgttgac aaagtgttcg ttgtttttgc cggatgcggc gtgaacgcct
    14341 tatccggcct acaaaatctc gtaaattcaa tatattgcac catcctcgta ggcctgataa
    14401 gcgtagcgca tcaggcaatt ctgtgtttgt catacgtaaa aaagggcgat cttccgaccg
    14461 ccctttttct tatttaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg
    14521 cttctttctc aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat
    14581 atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca
    14641 gaccgccgat agaagcacca ttgcccggtg ccgccgtcag gcgcgcggtg atcgggtcgc
    14701 ctgccagggt gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg
    14761 cggaagtcgc agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt
    14821 cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac
    14881 acatgatgat gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag
    14941 cccctgcgct ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc
    15001 atttgaaacc taccgggact tctaccagct tgcggcccaa gtcattgacc acacggtcga
    15061 tcatcgcaga agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat
    15121 gctggaacag gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg
    15181 gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat
    15241 cacgcagtgc cagcaggccc gccatcgcac actcggagga gcagtccata cggatcgcgc
    15301 cgtctttatc aaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca
    15361 ggttgaggtt gtaatactcg ccaatacgct tccagtattc gataccggaa ccgcccagcg
    15421 gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca
    15481 gaccttccac aaacggctgc accagatcct gctctttcac atgaccggat gccatcgctt
    15541 cgtcgaggga gatacgcttc acgcctttca ggccatcggc cagcagtgcg ttggccctgt
    15601 cttccaccac tttggtgacg ttggtatcag ccgggccacc atttggcgga ttgtacttga
    15661 taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcggac
    15721 cacctttttt attgtgaacc aggatggcat tggaaacggc aggcgttgga gtgaagccat
    15781 tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga
    15841 acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt
    15901 ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc
    15961 tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat
    16021 tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat
    16081 cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct
    16141 gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac
    16201 tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa
    16261 tacggcgtgc ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt
    16321 tcatctgccg caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac
    16381 gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc
    16441 ttttgctctg cgtattcgtc agaaagcaga agttcacgca ttgcgcgaac cttgtcttta
    16501 atcgttttga ccggcttcgc ttcggcgata gcaggtgacg caacgcgaac ctctttcgtc
    16561 accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat
    16621 gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact
    16681 tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta
    16741 tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa
    16801 accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc
    16861 gaaaaaaaga actttgccat gaaattgaat atccgcgcgc aaactgcaca aaaccagcac
    16921 aataattctc ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta
    16981 ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt
    17041 ctttcaccga gagatccggt aatgaattac ccggcgatgg cgcaggatct tgttgatact
    17101 ctggatgcac agcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg
    17161 gtaatggcac ttacggcact ggcccgcgat cgcatcgata aactggtggc gatcgatatc
    17221 gcaccggtcg actatcacgt acgccgtcat gatgagattt tcgcggctat caacgcggtc
    17281 agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat
    17341 gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac
    17401 gtgccggtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg
    17461 gatcaccctg ccctgtttat ccctagcggc aattctccgt atgttagcga gcagtaccgt
    17521 gatgatttac tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac
    17581 tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat
    17641 taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg
    17701 actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt
    17761 ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct
    17821 gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca
    17881 gctgcgtatt aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt
    17941 cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg agtcgcgcaa
    18001 tatgagccgt agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag
    18061 tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc
    18121 tgattccgaa agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc
    18181 cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg
    18241 gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag
    18301 acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttatgaca
    18361 ttctgctgct gggcatccca acctggtatt atggcgaagc gcagtgtgac tgggatgact
    18421 tcttcccgac tctcgaagag attgatttca acggcaaact ggttgcgctg tttggttgtg
    18481 gtgaccagga agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca
    18541 ttgaaccgcg cggtgcaacg atcgttggtc actggccaac tgcgggctat catttcgaag
    18601 catcaaaagg tctggctgat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc
    18661 agccggaact gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc
    18721 atctcgacga aattctcaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat
    18781 taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg
    18841 tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt
    18901 attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat
    18961 acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac
    19021 aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa
    19081 cgcttcctcg tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg
    19141 cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat
    19201 atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg
    19261 gtaaatccgt atttgaactg acacagcaac atcaccacga tcacctgatc tgcctcgact
    19321 gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg
    19381 caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag
    19441 gcgattgccg cgaagatgaa catgcgcacg aaggcaaata agccagcctg aatgagaaaa
    19501 agccaacctg cgggttggct tttttatgca agggaaataa gaaaagcgag agttacagct
    19561 ctcacttatt tgttattact gcgaatttcc tgccaaactt tatcacaatc ggctttcacc
    19621 gcctgatcat ttccggtttg cgttgcacgc agacactgct gataatccag tacgcggaca
    19681 ctttcctggt cggcaaaatg ctgatgttgt ttctctttct tcagcacgtt taacacgctc
    19741 tggcaggctt caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc
    19801 tctttcagac gactgtcttc ctgtggcgcg gttgattgcg cacaagctac cagccccgat
    19861 gccattatgg cgatgagaat cagttttttc atagcaaatt cctcaaacgt gcggcaggca
    19921 caaaacctgc cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac
    19981 gtcacgctca tcctggaaga tgttgccgta accaccgccc cagcttggga tgtcggagtg
    20041 gttgtcgtat tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat
    20101 ggtgtagacc gcatccaggc tgtatgcaga ctcttcaata gtccggtttt tgtcgtagta
    20161 cgcatccgga ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc
    20221 gccgatggcg aagcctagaa gattccagtt tttcaggtca tacatcgcac cgaagaagac
    20281 cgctttttcg ccgttagcgt tgaagtcgga acggttatcc caccagatat ccaggcggcc
    20341 gttggaggaa gcgtaggctg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc
    20401 cttaacccag gtgccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt
    20461 caacgcttgc agccaggcgg tgccgtcata aaggtcgttg acgctgcgat cgtcaacttt
    20521 atcgcgcgta ccatagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt
    20581 gtagctggct ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgccgcttc
    20641 cagtacgaag ttatttttga agtcgtattt cgccccaagg gagtgcagat aatcaacttt
    20701 ggtggtttta tcgttctgat aaaactcatc catttccaga tgccacggcg ctttgtattc
    20761 gttggtccac atgtaggaga aactcaacgc accagcatcg ccgtaatcaa aattcgcccc
    20821 ggcttccgcc ccctgataag tacctggcat aaagctccag tgcggcgcta acagcgtttg
    20881 accagttggc tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc
    20941 agctttatac aggcttatac cgcttttgtc gccggaccag tcttcgtcat aggctttatt
    21001 actttttgaa aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat
    21061 cgccgtaaac acagcaatat caaggccgaa catatcagca gcataaccgg actgaaaatc
    21121 gaggttggca ttccaggtgg agtgagaaag gttggttttg tatttgtcgc cgtcggtaac
    21181 atctttacgg tcgcgttcac gctgccagta atatataccg ccggttaagg ttgaatcgtc
    21241 gatgaatcct tcggcgcgag cctccggcat tgccataaag cccgacattg ctgtaacacc
    21301 ggcgatagcc agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc
    21361 tttgacgtat aaattgctgc accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt
    21421 agcattgaga aaagtctcgc tgactacagg aatagactat ttttcgcgac gcgaattatc
    21481 aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt tgatgatttt
    21541 tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagc gtaaatttcc
    21601 agcaagatga aagcggttac attttattta attatggttt gtgattttaa atttatttta
    21661 tggataaatt gcgaagcgga attaattcgc gaagaaaagc agaaaaaacg ccgcagagtg
    21721 cggcgtttgg gattgtttgc tatttcttgc cggatgcgac gtaaatgcct cacccggcct
    21781 ggtagaactt aaaattactc gcctactttc gcccaggtat cacgcagccc aacggtgcgg
    21841 ttaaataccg gtttttccgc tgtggaatgg cggctgtcga ggcagaagta accttcacgc
    21901 tcaaactgga atgctttacc cgcaaccgca tctttcagcg acggttcagc aaagccctgt
    21961 ttgatcacca gcgattccgg gttaatcacc gacaggaaat catccgcagc acctgggtta
    22021 ggcacgctga acagacggtc gtacagacgg atttcaaccg gcagcgcatg tgccgcgctc
    22081 acccagtgaa taacaccttt gactttacga ccatctgccg gatctttgct taaggtgtcg
    22141 gcgtcataag tacagaagat ggtggtgata ttgccttcgg catctttctc cacgcgttct
    22201 gccttaatga cgtaagcatt acgcagacgc acttctttac ccagcaccag acgtttgtac
    22261 tgcttgttag cttcttcgcg gaaatcggcg cgatcgatcc aaatctcact gctaaacggc
    22321 acctggcggc tacccatttc cggtttgttc ggatggttcg gcatggtaac catttcgcct
    22381 tcgccctgat agttttcgat aaccagtttc accggatcga taaccgccat tgcgcgcggg
    22441 gcgttttcgt tgagatcttc acggatgcag gattccagcg acgccatctc aatggtgttg
    22501 tcctgcctgg tcacgccgat gcgtttgcag aactcacgaa tagaagcagc agtgtaacca
    22561 cgacgacgca gaccggaaat ggtcggcata cgcgggtcat cccagccttc aacgtgcttg
    22621 tcggtcacca gcaggttcag cttacgcttg gacatcacag tgtattccag attcaggcgc
    22681 gagaattcgt actggcgcgg gtgaacagga atggtgatgt tgtccagcac ccagtcgtac
    22741 aggcgacggt tgtcctggaa ctcaagcgta cacagagagt gcgtaatacc ttccagggca
    22801 tcgctgatgc agtgggtgaa gtcgtacatc gggtagatgc accacttgtt gccagtctgg
    22861 tggtgttcag caaatttaat gcggtacagc accggatcgc gcatcacgat aaacggtgaa
    22921 gccatgtcga ttttcgcacg caggcaggct ttaccttctt caaaaccacc ggcacgcatt
    22981 ttttcgaaca gcgccaggtt ctcttcaacg ctgcggtcgc ggtacgggct gtttttaccc
    23041 ggttgcgtca gggtgccgcg gtattcgcgg atctgttccg gcgtcagttc atcaacgtac
    23101 gccaggcctt tattgatcag ttcgatcgca taggcgtgga gctgatcaaa ataatcggag
    23161 gagtaacgga cgttaccaga ccagtgaaaa cctaaccact ctacgtcgtt tttgatcgac
    23221 tcaacatact cgatatcttc ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg
    23281 cctttatagt cctgggcgat cccgaagttc aggcagatag atttcgcatg gccaatatgc
    23341 agatagccat tcggctccgg cgggaaacgg gtgtgtactg tggtgtgctt accactggcc
    23401 agatcttcat cgatgatctg acggataaag ttagtcgggc gggcttctgc ctcactcatc
    23461 gtggattcct caaagcgtaa acaacgtata acggcgtatg atcttataag cggaacaggc
    23521 tgacaactgt tagtttttta aaattgataa tctaacaagg attatgggga tgattgatga
    23581 aaaaaaaagc ggcagagaag atctccaccg cttgtcattg ttggatgcga cgctcaagcg
    23641 tcgcatcagg cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac
    23701 aacatggccc tgagctttaa tgatcaagcc actgaaatcg tcgatattgc tgcaaaccac
    23761 cgggctaatc atcgagcggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg
    23821 ttgccctgcg cttacctgcg caccctcttc caccagacgt ttaaagcctt taccttccag
    23881 cgctacggtg tcgataccca tatggacgac gatctccgcg cctttttcgg tttccaggca
    23941 gaacgcgtgg ttggtgttga agattttcac gattgtccct gcggctggtg atacgacgat
    24001 tttatctgtc ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc
    24061 aggaacctga tccagtgcca cgacatcacc ggtaatcggc gataccagct ccgcgataga
    24121 taccgcgttt ggtacagcct gcggttttgc tacaggcgcg gcagttgacg gagttgcttc
    24181 agctgacgca gcggctaccg gaccacgggc aacgactttc ttcatcgcat cgccgatgga
    24241 ttctgctttc gcgccaacaa tcacctgaat agtctgtttg ttcagtttca ctacaccaga
    24301 agcacccaga cgtttacaca tcgtatcgtt gacgcgggca gagtcagcca cggtcaggcg
    24361 cagacgggtg atacaggcgt caattgcttt caggttgtca gtgccgccaa ccgcagcaat
    24421 atagttggtc gccagttgat tgaggccttc ttcagtgttg ctgttggctt cttcagtaac
    24481 gatctcatct tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa
    24541 actgaacacc acgaagtaga tagcgaagaa gacaacaccc atcaccagca gcatccagac
    24601 gttctggctg gcggccggca ggttatacat caacgcgtag tcgatagccc ccgcagagaa
    24661 agagaagcca gcatggatac ccagcagtgt tgccacaaac aggctgatac cggtcagcag
    24721 tgcgtgcagg aggtacagca gcggtgcaag gaacatgaac aggaattcca gcggctcagt
    24781 cacaccggtc aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg
    24841 acgctctttc ggtgctgcga agtacatcgc cagcgccgca cccggcagac cgaacatcat
    24901 aatcgggaag aagccggaca tgaacatccc cgcggtgccg tcaccggcat agaagcggtt
    24961 gatatcaccg tggaaaaccg tacccgccgc gttggtgaat tcaccaatct ggaaccaggc
    25021 gatggtgttc agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa
    25081 gataccggaa cccagcgcgc ccgcagaaac gatccactcg cctcctgcat ggatagcgtg
    25141 ctgtaccggc ggccagacgt aaccaaaaat ggccgccagc accaggcaga agaagccggt
    25201 ggcaatcggc acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc
    25261 ggaccaacgg ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc
    25321 catgtttatt tctgggttga tggtcaccat cgctttggtt aacacaaagt aacctaccgc
    25381 acccgccagc gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca caccgatggc
    25441 gaagattaat gcgaggttat caaaaatcgc accgcccgcc tgggcaataa acgcaacgtt
    25501 aagtaaatct ggctgaccga atcgcagcaa cagtgccgcc accggcagca ccgcgatagg
    25561 gagctgtaac gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct
    25621 acgagaaccc tatttggctc gtttaaagcc gtatttttat tttgctgcaa attgtactgc
    25681 cgatgttctg taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg
    25741 tatcgcaaat taaacgcgtg tcttttgtga gttttgtcac caaatatcgt tatcatcact
    25801 cccttttact ggctaaacca gaaaacttat tttatcattc aaaaaatcag gtcggattga
    25861 cgcctgtctg cgcaaatcca agttacgctt aaagatgcct aatccgccaa cggcttacat
    25921 tttacttatt gaggtgaata atgagactga tccccctgac taccgctgaa caggtcggca
    25981 aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact gccgatcgtc
    26041 cgtttgttct gggcctgcca actggcggca cgccgatgac cacctataaa gcgttagtcg
    26101 aaatgcataa agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat
    26161 atgtcggtct gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct
    26221 tcgatcacgt tgatattcca gcagaaaaca tcaaccttct caacggcaac gccccggata
    26281 tcgacgccga atgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt
    26341 ttatgggcgg tgtaggtaac gacggccata ttgcatttaa cgaaccggcg tcttctctgg
    26401 cttctcgtac tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct
    26461 ttgataacga tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacattgc
    26521 tggatgccga agaagtgatg attctggtgc tgggtagcca gaaagcactg gcactgcagg
    26581 ctgcagttga aggttgcgtg aaccacatgt ggaccatcag ctgtctgcaa ctgcatccaa
    26641 aagcgatcat ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat
    26701 atttcaatga attagaagca gaaaatatca aaggtctgta attgttatcc ctgccctggc
    26761 tccttgctca gggcaatatt ttttaaaatc gggggtcaga atgtatgcat taacccaggg
    26821 ccggatcttt accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct
    26881 gattaaaagc gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa
    26941 cggggccatt ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca
    27001 gtttaacgac accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa
    27061 tgagaaatca ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat
    27121 gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaga catccgaatc aggcgttagg
    27181 tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt
    27241 tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg acgtcattac
    27301 caaagtgacc ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc
    27361 cgggattgtg gtttctgccg gtcactccaa cgcgacgttg aaagaagcga aagccggttt
    27421 ccgcgcgggg attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg
    27481 tgaaccgggc ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc
    27541 tgatggcctg catgttgatt acgccaacat tcgtaacgct aaacgcctta aaggcgacaa
    27601 actgtgtctg gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt
    27661 tgcgggtaaa acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag
    27721 cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc
    27781 actggatgaa gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg gcgttgagaa
    27841 acgtctcggc acactcgccg caggtaaagt agccaacctg accgcattca cacctgattt
    27901 taaaatcacc aagaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg
    27961 acaccaggcg gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg
    28021 gcggtttatc gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa
    28081 agccagcttg cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg
    28141 atcaaagaag ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc
    28201 gaaacccgca atttccacgc aatcggcgta cggcttggtc gtcacgacgc caccatcacc
    28261 ctgtttgatc tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc
    28321 cagcaaacgc tggaacatgc cctgttgaat gccattgctc agtttattga tagctaccag
    28381 cgcaaactgc gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt tgacccggac
    28441 agcggcaaaa ttcattacat gccgcatatt caggtagaaa actgggggct ggtagaagct
    28501 ctggaagaac gttttaaagt gacctgtttc gttggtcacg atatccgtag tctggcgctg
    28561 gcggagcact acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat
    28621 cgcggaaccg gggccgggat tatctctaac gggcgcattt ttattggccg caacggtaac
    28681 gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac
    28741 tttggctgcc tggaaactat cgccgccaac gctgccattg aacaacgggt gttgaatctg
    28801 ttaaagcagg gctaccagag ccgcgtgccg ctggacgact gcaccatcaa aactatctgc
    28861 aaagccgcga acaaaggcga tagtctggcg tcggaagtaa ttgagtatgt cggtcgtcat
    28921 ctgggtaaaa ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgcc
    28981 ggtgaaatca ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc
    29041 caggcgctga aggcgtttcg cactaatctg ccggtggtac gttctgagct ggatcaccgc
    29101 tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag
    29161 catttgctgg aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt
    29221 agtccatgac cattaaaaat gtaatttgcg atatcgatgg cgtgctgatg cacgataacg
    29281 tcgccgtacc gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg
    29341 tgttgctgac caactatcct tcgcagactg ggcaagatct ggcgaaccgc tttgccaccg
    29401 caggtgtcga tgtacctgac agcgtgtttt atacctctgc gatggcgact gccgatttcc
    29461 tgcgtcgcca ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg attcatgaac
    29521 tgtacaaagc cggtttcact attaccgatg tgaaccctga tttcgtgatt gttggcgaaa
    29581 cgagttccta caactgggac atgatgcata aagcagccta tttcgtcgct aacggtgcac
    29641 gttttatcgc caccaatccg gacacccacg ggcgcggttt ttatcccgcg tgtggcgcgt
    29701 tgtgtgcagg catcgaaaaa atctccgggc gtaaaccgtt ctatgttggt aaaccgagtc
    29761 cgtggatcat ccgcgcggca ttaaacaaaa tgcaggcgca ttcggaagaa acggtgattg
    29821 tcggcgataa cctgcgtacc gatattctgg cgggcttcca ggcaggtctg gaaacgattc
    29881 tggtgctttc tggtgtttca tcgctcgacg atatcgacag tatgccgttc cgcccaagct
    29941 ggatttaccc gtcggtcgct gaaatcgacg ttatctgaat agcaaccacg tctgcggacg
    30001 tggttggctc tgatgacaaa cgccaaactg cctgatgcgc tacgcttatc aggcctacgc
    30061 agctcctgca atatattgaa ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc
    30121 atccggcatg aacaaattgc actttgtcag caatttggcc cccgtaaaat acaaacattc
    30181 aataaaaccc gctgaaaatt atcgattcat caataaatat atcctcacac cagacctttt
    30241 ttcaccaatc gacaatcacc attacgtttt ttattttttc accgccaaat cgcttgcgca
    30301 cagtgctgct ttgcggcatt tttttaaaca agcaaacaca acaagcaaca aataccagat
    30361 taacggagaa ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca gacgcagttg
    30421 agctgcgtaa gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt
    30481 ccggtattta tgccagcgat aacgccattc tcgctcacga acgtctgtca attgttgacg
    30541 ttaacgcggg ggcgcaacct ctctacaacc aacaaaaaac ccacgtactg gcggtaaacg
    30601 gtgaaatcta caaccaccag gcattgcgcg ctgaatatgg cgatcgttat cagttccaga
    30661 cagggtctga ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg
    30721 acgacttgca gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcctacctga
    30781 ttggtcgcga ccatctgggg atcatcccac tgtatatggg ctatgacgaa cacggtcagc
    30841 tgtatgtggc ctcagaaatg aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc
    30901 cggcggggag ctatttgtgg agccaggacg gcgaaatccg ttcttattat catcgcgact
    30961 ggttcgactt tgatgcagtg aaagataacg tgaccgacaa aaacgagctg cgtcaggctc
    31021 tggaagattc cgttaaaagc catctgatgt ctgatgtgcc ttacggtgtg ctgctttctg
    31081 gtggtctgga ttcctcaatt atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg
    31141 aagatcagga acgctctgaa gcctggtggc cgcagttaca ctcctttgct gtaggtctgc
    31201 cgggttcacc ggatcttaaa gcagcccagg aagtggcaaa ccatctgggc acggtgcatc
    31261 acgaaattca cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca
    31321 tcgaaactta tgatgtgacc actattcgcg cttcaacacc gatgtattta atgtcgcgta
    31381 agatcaaggc gatgggcatt aaaatggtgc tgtccggtga aggttctgat gaagtgtttg
    31441 gcggttatct ttacttccat aaagcgccca acgctaaaga actgcatgaa gagacggtgc
    31501 gtaaactgct ggccctgcat atgtatgact gcgcgcgcgc caacaaagcg atgtcagcct
    31561 ggggcgtgga agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca
    31621 ttaacccgca ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat
    31681 gttttgagtc atacctgccc gcaagcgtgg cctggcggca gaaagagcag ttctccgatg
    31741 gcgtcggtta cagttggatc gacaccctga aagaagtggc ggcgcagcag gtttctaatc
    31801 agcaactgga aactgcccgc ttccgcttcc cgtacaacac gccaacctca aaagaagcgt
    31861 atctgtaccg ggagatcttt gaagaactgt tcccgcttcc gagcgccgct gagtgcgtgc
    31921 ctggcggtcc ttccgtcgcg tgttcttccg ctaaagcgat cgaatgggat gaagcgttca
    31981 agaaaatgga cgatccatct ggtcgtgcgg ttggtgttca ccagtcggca tataagtaag
    32041 aaatgcgaaa cagccccggt gaaatccggg gctgtttcag ttattgaggt gcttgttgat
    32101 tctgatatgc agtaaggaaa tgttccgggt ttgccacatc actgtttgac aactcatcac
    32161 tgtaggccag tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc
    32221 ctgtacgaca aaagaatggc tgtagggtgg ggaaaaaaca caaagtaaac aataattgac
    32281 gaatatagcg ccacgctgtt cgcaacctaa ccaaatagtc actttcgagc aattttcctt
    32341 gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag
    32401 gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc actcataatg
    32461 atggggtcac aggttcgaat cccgtcgtag ccaccatctt tttggcggga gtggcgaaat
    32521 tggtagacgc accagattta ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct
    32581 cccgcaccat tcaccagaaa gtgttgtacg gatggggtat cgccaagcgg taaggcaccg
    32641 gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatct tcttcgagta
    32701 agcggttcac cgcccggtta ttggggtatc gccaagcggt aaggcaccgg tttttgatac
    32761 cggcattccc tggttcgaat ccaggtaccc cagccataga agaaacaatc tggctacgta
    32821 gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt
    32881 agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg
    32941 gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt cgaaccctcg
    33001 taccccagcc aatttattca agacgcttac atcgtaagtg cacccagttg gggtatcgcc
    33061 aagcggtaag gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag
    33121 ccacattaaa aaagctcgct tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga
    33181 atgcgatgtt gacacgtctt atccttcaat gtcggatgcg acgctgccgc gtcttatccg
    33241 acctacggtt ggcacgcatc cggcaatgtt gtaaggctac aaccctaacg catatttcag
    33301 cgcctgacgt ttcaacacgc cagcacgctc tgccgccatt aacccgagat tacgcacaaa
    33361 acgcagcggc ggcagattat tactgaatcc ggcataaaac agatccatac cactttgcat
    33421 aatgaagtta tccaccatgc gccgcatctg gtaacgcttg aggacaggat aactggccca
    33481 cgcttcaccg taactgcggg cattcaccag aacatcaatc agggcatcga catcacgata
    33541 accaagattc accccctgcc ccgccagcgg atggatggta tgcgcggcat cgcccaccag
    33601 cgcaagcccc ggctgcacat actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc
    33661 ggcaagcggt gtaacgtaac ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag
    33721 ttgcgccata ttcatattct gcaactggcg aatacgcgcc ggagagtcat accacaccag
    33781 cgatgcccag ttatcaaaca acggcagaaa cgcacgcggt ccgtccgggg taaattgctg
    33841 ccaggtgctg tcgccgggat cgttctcaca ctgtacgcta atcaacatgc acgactgcgc
    33901 atactgccag gcatgaacgc caattcccgc catctgccgc acctgagaat ttgcgccgtc
    33961 ggcaccaatc accagtttcg cgcggatcgt ttcaccgccc ttcagttcca gctcctgaag
    34021 atcattatct cgatgtaatg caatcagcga gcctggcaca cgtaacgtta ctttcggatg
    34081 cgcttccagc gcctgccaca gcgcctgttg cagtacggtg ttttccacca tatagccaag
    34141 caacggtagc ttaagttcag cggcgtcaaa caccacatgc gccgtttccc actcccacgt
    34201 ttccagtctg cggtaaggat ggcaacgcat agcctgtact gcatcccaga cccctaaccc
    34261 tttaagcaat gataccgatg cggcgctgat cgccgagatc cgcacgtccg gttggctatc
    34321 agcgacaaac ggtgcaggtt ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc
    34381 cagccccagc gccagcgcgc cgccgaccat tcctccgccg acaatggcaa tttccgttgg
    34441 ttgatttgtc atggtcaatc atcctgttag caatatgctt taagtttaca ggatttttac
    34501 ccccaagggc tgataacgct catactggcc acaacggcag caaagcatta cactatgcgc
    34561 cctgcattcc tggctactat ttcgcaagag caagtcgatg accaaaaaac tccatattaa
    34621 aacctggggc tgtcagatga acgagtacga ttcatcgaag atggccgatc tgctggatgc
    34681 cacccacggc tatcaactga ccgacgtggc ggaagaagcg gatgtgctgc tgctgaacac
    34741 ctgctcaatc cgcgagaagg ctcaggaaaa agtcttccat cagttgggtc gctggaaact
    34801 gttaaaagag aagaatccag acctgattat cggcgtcggt ggctgcgtgg catcgcaaga
    34861 aggcgagcac attcgccagc gcgcccacta tgtcgatatt atttttgggc cgcaaacgct
    34921 gcaccgtctg ccggagatga tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat
    34981 cagcttcccg gaaatcgaga agtttgaccg tctaccggaa ccgcgcgccg aagggccgac
    35041 cgcgtttgtc tccatcatgg aaggctgcaa taaatattgc acctactgcg tggtgcctta
    35101 cacccgtggt gaagaggtaa gccgtccgtc tgacgatatt ctgtttgaga ttgcccagct
    35161 tgcggctcag ggcgtgcgtg aagtcaacct gctcggtcag aacgtgaacg cctggcgtgg
    35221 tgaaaactac gacggctcca ccggatcgtt tgccgatctg ctgcgtctgg ttgctgcgat
    35281 cgacgggatc gatcgtattc gctttaccac cagccatccg atcgaattca ccgacgatat
    35341 catcgaagtg tatcgcgaca cgccggagct ggtgagcttc ctgcatctgc cggtacagag
    35401 cggttccgat cgcattctga acctgatggg ccgtactcat acggcactgg agtacaaagc
    35461 gatcatccgt aaactgcgtg cggcgcgtcc agatattcag atcagttctg atttcatcgt
    35521 tggcttccct ggcgaaacca ccgaagactt cgagaaaacg atgaagctga ttgccgacgt
    35581 caatttcgac atgagctaca gctttatctt ctctgcacgt ccgggtacac cagccgccga
    35641 tatggttgat gatgttccgg aagaagagaa gaagcagcgt ctgtatattc tgcaagaacg
    35701 tatcaatcag caagcgatgg catggagccg ccggatgctc ggcaccaccc agcgtattct
    35761 ggtagaaggg acttcgcgta agagcatcat ggagctttcc gggcgtaccg aaaataaccg
    35821 cgtggtcaac ttcgagggca caccggatat gatcggtaaa ttcgtcgatg tagaaattac
    35881 cgacgtctac ccgaactctc tgcgcggtaa agtggtgcgt actgaagatg aaatgggcct
    35941 gcgcgtggca gaaacaccgg aatcggtgat tgcccgtacc cgcaaagaaa acgaccttgg
    36001 cgtgggttat tatcagccgt aattctcagg cccgccgttc cggtgggcct tgttttttct
    36061 ctctttatcc ccatctttat cgcaatgctt gcgccttttt cctgtggcgt gaataatttc
    36121 cttataggcc ggtgaatgtt cagcttccgg cggcatacag ataagaggaa cggtttgaac
    36181 atagacactc gcgaaatcac cctggagcca gcagacaacg cgcgtctgtt gagcctgtgc
    36241 ggcccgtttg atgacaacat caagcagctc gaacgccgtc tcggcatcga gatcaatcgc
    36301 cgcgacaacc actttaaact gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt
    36361 ctgcgtagcc tgtatgtcga tacagccccg atgcgcggtc agattcagga tatcgaaccg
    36421 gaacagatcc accttacgat taaagaagcg cgagtactgg agcaaagtgc ggagagcgtg
    36481 ccggagtacg gcaaagcggt caatatcaaa accaaacgcg gcgtaattaa gccgcgcacg
    36541 ccaaaccagg cgcagtacat cgccaatatt ctcgaccatg acattacctt cggcgttggc
    36601 ccggcgggta cgggtaaaac ctacctggca gtggctgcgg cagttgatgc cctggagcgt
    36661 caggagattc gccgtattct gctgactcgt ccggcggtcg aagccggtga gaaactgggc
    36721 ttcctgcctg gcgatttaag ccagaaagta gacccgtatc tgcgcccact gtacgacgcg
    36781 ctgtttgaaa tgctgggctt tgagaaagtt gagaaactga ttgagcgcaa cgttattgaa
    36841 gtcgcgccgc tggcctatat gcgtggtcgt acgctgaacg acgcgtttat cattctcgat
    36901 gagagccaga acaccaccat cgaacagatg aagatgttcc tgacccgtat cggttttaac
    36961 tcaaaagcgg ttatcaccgg cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca
    37021 ggcttacgtc acgccatcga agtactggcc gatgtcgaag agatcagctt taacttcttc
    37081 cacagcgaag acgtggttcg tcaccctgtg gtggcgcgta tcgttaacgc ctatgaagcc
    37141 tgggaagaag ccgaacaaaa acgaaaagcg gcgctggcgg cagaacgcaa gcgcgaagaa
    37201 caggaacaaa aatgagtcag gtgatcctcg atttacaact ggcatgtgaa gataattccg
    37261 ggttaccgga agagagccag tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg
    37321 aagaatcgga agtgactatt cgcgtggtcg ataccgccga aagccacagt ctgaatctga
    37381 cctatcgcgg taaggataag ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg
    37441 gcatggaaat gtcgctactg ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag
    37501 ctcaggagca aggcaaacca ctggaggcgc actgggcgca tatggtggtg cacggcagtc
    37561 tgcatttgtt aggttacgat cacatcgaag atgacgaagc agaagaaatg gaagccctcg
    37621 aaacagagat tatgcttgct ctgggctatg aggatccgta cattgccgag aaagaataat
    37681 ttgccaggca tttgactggc agacgtcccc gccgttggtg attaacgacg gcgttttgat
    37741 taactaacat gagaacccat acgacgccat gagcgacgac aattcacaca gtagtgacac
    37801 gataagcaac aagaagggat ttttctccct gttactcagc caacttttcc acggtgaacc
    37861 gaaaaaccgt gacgaactgc tggcgctgat ccgtgattcc gggcagaacg accttatcga
    37921 cgaagatacg cgcgatatgc tcgaaggggt gatggacatc gcagaccaac gcgttcgcga
    37981 catcatgatc ccccgctccc agatgattac cctgaaacgc aaccagacgc tggacgaatg
    38041 ccttgatgtc atcatcgagt ccgcccactc acgtttcccg gtgattagcg aagacaaaga
    38101 tcacattgaa gggattctga tggcgaaaga cttgctgccg tttatgcgca gcgatgctga
    38161 agccttcagc atggacaaag tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt
    38221 agaccggatg ctgaaagagt ttcgctctca gcgttaccac atggcgatcg tcattgacga
    38281 attcggtggg gtttccggtc tggtgaccat tgaagacatc ctggaactga ttgttggtga
    38341 gattgaagac gaatatgacg aagaagatga tatcgacttc cgtcagctga gtcgtcatac
    38401 ctggaccgtg cgcgcactgg cttccattga agacttcaac gaagcgttcg gcacccactt
    38461 tagcgatgaa gaagtcgaca ctatcggtgg tctggtgatg caggcgtttg ggcatcttcc
    38521 ggcgcgtggc gaaactatcg acatcgacgg ttaccagttc aaagtggcga tggccgacag
    38581 tcgacgtatt attcaggttc atgtcaaaat cccggatgac tcaccccagc cgaagctgga
    38641 tgaataaaac cgaaactgga tagataacta catggctttt gcctcattaa ttgaacgcca
    38701 gcgcattcgc ctgctgctgg cgttattatt cggtgcctgc ggaacgctgg ccttctctcc
    38761 ttacgacgtc tggcctgcgg cgattatttc gttgatgggg cttcaggcgt tgacctttaa
    38821 ccgccgtcca ctccagtctg ccgctattgg cttttgctgg ggatttggcc tctttggcag
    38881 cggtattaac tgggtctatg tcagcatcgc gacctttggc ggaatgcctg gcccggttaa
    38941 catcttcctg gtggtgctgc tggcggcgta tttgtcgctg tataccggac tgtttgctgg
    39001 cgtgctgtcg cgtctgtggc cgaaaaccac ctggctgcgc gtggcgattg ccgcccctgc
    39061 tctctggcaa gtgaccgagt ttctgcgcgg ttgggtactg acaggcttcc cgtggttaca
    39121 gttcggctat agccagattg atggcccgtt aaaagggctg gcaccgataa tgggcgtgga
    39181 agccattaac ttcctgctga tgatggttag tggcctgctg gcactggcgt tggtcaaacg
    39241 caactggcgt ccgctggtgg tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta
    39301 catccaatgg tttaccccgc aaccggagaa aaccattcag gtttcgatgg ttcagggcga
    39361 tattccgcaa tcactgaaat gggatggaga tcagcttctt aatacgctga agatttacta
    39421 caacgcaacg gctccgctga tgggcaaatc atcgttgatt atctggccgg agtcggcgat
    39481 aaccgatctg gaaattaatc agcaaccgtt cctcaaagca ctggacggtg agttgcgcga
    39541 taaaggtagc tcgctggtaa ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta
    39601 cgatacctac aacaccatca tcacactggg taaaggtgca ccgtacagct acgaatcagc
    39661 cgatcgctat aacaaaaacc atctggtgcc gtttggcgag tttgtcccgc tggagtcgat
    39721 tctgcgtccg ttagcaccgt tctttgatct gccgatgtcg tcgttcagcc gtgggccata
    39781 tatccagccg ccgctgtcag taaatggtat tgagcttact gcggctattt gctacgagat
    39841 cattctcggc gagcaagtgc gcgataactt ccgcccggat accgactatc tgctgactat
    39901 ctccaacgat gcgtggtttg gtaaatctat tggtccatgg caacacttcc agatggcgcg
    39961 aatgcgtgcg ctggagctgg cgcgcccact gttgcgcagc accaacaacg gcattacggc
    40021 ggtgattggc ccgcagggtg agattcaggc gatgatcccg cagttcaccc gcgaggtgtt
    40081 aaccactaat gtgacaccga ccaccggact cacaccgtat gcacgtaccg gcaactggcc
    40141 actgtgggtg ctgacggcac tgtttggttt tgccgc
//
LOCUS       DAJVTY010000014        39729 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVTY010000014 DAJVTY010000000
VERSION     DAJVTY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39729
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.014"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            7..438
                     /locus_tag="OQQ66_000712"
     CDS             7..438
                     /locus_tag="OQQ66_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1903738.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            521..1423
                     /locus_tag="OQQ66_000713"
     CDS             521..1423
                     /locus_tag="OQQ66_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS1903739.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1468..3630)
                     /gene="uvrD"
                     /locus_tag="OQQ66_000714"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1468..3630)
                     /gene="uvrD"
                     /locus_tag="OQQ66_000714"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1903740.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3714..4430)
                     /gene="yigB"
                     /locus_tag="OQQ66_000715"
     CDS             complement(3714..4430)
                     /gene="yigB"
                     /locus_tag="OQQ66_000715"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1903741.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4430..5326)
                     /gene="xerC"
                     /locus_tag="OQQ66_000716"
     CDS             complement(4430..5326)
                     /gene="xerC"
                     /locus_tag="OQQ66_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1903742.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5323..6030)
                     /locus_tag="OQQ66_000717"
     CDS             complement(5323..6030)
                     /locus_tag="OQQ66_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1903743.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6027..6851)
                     /gene="dapF"
                     /locus_tag="OQQ66_000718"
     CDS             complement(6027..6851)
                     /gene="dapF"
                     /locus_tag="OQQ66_000718"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1903744.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7090)
                     /locus_tag="OQQ66_000719"
     CDS             complement(6896..7090)
                     /locus_tag="OQQ66_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1903745.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            7553..7873
                     /gene="cyaY"
                     /locus_tag="OQQ66_000720"
     CDS             7553..7873
                     /gene="cyaY"
                     /locus_tag="OQQ66_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1903746.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7913..10459)
                     /gene="cyaA"
                     /locus_tag="OQQ66_000721"
     CDS             complement(7913..10459)
                     /gene="cyaA"
                     /locus_tag="OQQ66_000721"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1903747.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10846..11787
                     /gene="hemC"
                     /locus_tag="OQQ66_000722"
     CDS             10846..11787
                     /gene="hemC"
                     /locus_tag="OQQ66_000722"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1903748.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11784..12524
                     /gene="hemD"
                     /locus_tag="OQQ66_000723"
     CDS             11784..12524
                     /gene="hemD"
                     /locus_tag="OQQ66_000723"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1903749.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12546..13745
                     /gene="hemX"
                     /locus_tag="OQQ66_000724"
     CDS             12546..13745
                     /gene="hemX"
                     /locus_tag="OQQ66_000724"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1903750.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            13748..14944
                     /gene="hemY"
                     /locus_tag="OQQ66_000725"
     CDS             13748..14944
                     /gene="hemY"
                     /locus_tag="OQQ66_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1903751.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15623..15784
                     /locus_tag="OQQ66_000726"
     CDS             15623..15784
                     /locus_tag="OQQ66_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903752.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15868..17103)
                     /locus_tag="OQQ66_000727"
     CDS             complement(15868..17103)
                     /locus_tag="OQQ66_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1903753.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17250..17326)
                     /locus_tag="OQQ66_000728"
     tRNA            complement(17250..17326)
                     /locus_tag="OQQ66_000728"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17290..17292),aa:Pro,seq:tgg)
     gene            complement(17369..17455)
                     /locus_tag="OQQ66_000729"
     tRNA            complement(17369..17455)
                     /locus_tag="OQQ66_000729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17419..17421),aa:Leu,seq:cag)
     gene            complement(17476..17551)
                     /locus_tag="OQQ66_000730"
     tRNA            complement(17476..17551)
                     /locus_tag="OQQ66_000730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17516..17518),aa:His,seq:gtg)
     gene            complement(17610..17686)
                     /locus_tag="OQQ66_000731"
     tRNA            complement(17610..17686)
                     /locus_tag="OQQ66_000731"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17650..17652),aa:Arg,seq:ccg)
     gene            complement(17789..19174)
                     /locus_tag="OQQ66_000732"
     CDS             complement(17789..19174)
                     /locus_tag="OQQ66_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1903754.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19365..20105)
                     /gene="rffM"
                     /locus_tag="OQQ66_000733"
     CDS             complement(19365..20105)
                     /gene="rffM"
                     /locus_tag="OQQ66_000733"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1903755.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20108..21460)
                     /gene="wzyE"
                     /locus_tag="OQQ66_000734"
     CDS             complement(20108..21460)
                     /gene="wzyE"
                     /locus_tag="OQQ66_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1903756.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21457..22536)
                     /gene="rffT"
                     /locus_tag="OQQ66_000735"
                     /gene_synonym="wecF"
     CDS             complement(21457..22536)
                     /gene="rffT"
                     /locus_tag="OQQ66_000735"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1903757.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22533..23783)
                     /gene="wzxE"
                     /locus_tag="OQQ66_000736"
     CDS             complement(22533..23783)
                     /gene="wzxE"
                     /locus_tag="OQQ66_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1903758.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23785..24915)
                     /gene="rffA"
                     /locus_tag="OQQ66_000737"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23785..24915)
                     /gene="rffA"
                     /locus_tag="OQQ66_000737"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1903759.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24920..25594)
                     /gene="rffC"
                     /locus_tag="OQQ66_000738"
                     /gene_synonym="wecD"
     CDS             complement(24920..25594)
                     /gene="rffC"
                     /locus_tag="OQQ66_000738"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1903760.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25572..26453)
                     /gene="rfbA"
                     /locus_tag="OQQ66_000739"
     CDS             complement(25572..26453)
                     /gene="rfbA"
                     /locus_tag="OQQ66_000739"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1903761.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26472..27539)
                     /gene="rffG"
                     /locus_tag="OQQ66_000740"
     CDS             complement(26472..27539)
                     /gene="rffG"
                     /locus_tag="OQQ66_000740"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1903762.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLIYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27539..28801)
                     /gene="wecC"
                     /locus_tag="OQQ66_000741"
     CDS             complement(27539..28801)
                     /gene="wecC"
                     /locus_tag="OQQ66_000741"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1903763.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28798..29928)
                     /gene="wecB"
                     /locus_tag="OQQ66_000742"
     CDS             complement(28798..29928)
                     /gene="wecB"
                     /locus_tag="OQQ66_000742"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1903764.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29984..31030)
                     /gene="wzzE"
                     /locus_tag="OQQ66_000743"
     CDS             complement(29984..31030)
                     /gene="wzzE"
                     /locus_tag="OQQ66_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1903765.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31042..32145)
                     /gene="wecA"
                     /locus_tag="OQQ66_000744"
     CDS             complement(31042..32145)
                     /gene="wecA"
                     /locus_tag="OQQ66_000744"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1903766.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMALGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32385..33644)
                     /gene="rho"
                     /locus_tag="OQQ66_000745"
     CDS             complement(32385..33644)
                     /gene="rho"
                     /locus_tag="OQQ66_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1903767.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33729..33830)
                     /gene="rhoL"
                     /locus_tag="OQQ66_000746"
     CDS             complement(33729..33830)
                     /gene="rhoL"
                     /locus_tag="OQQ66_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1903768.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33971..34300)
                     /gene="trxA"
                     /locus_tag="OQQ66_000747"
     CDS             complement(33971..34300)
                     /gene="trxA"
                     /locus_tag="OQQ66_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1903769.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34431..35696
                     /gene="rhlB"
                     /locus_tag="OQQ66_000748"
     CDS             34431..35696
                     /gene="rhlB"
                     /locus_tag="OQQ66_000748"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1903770.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35830..37314
                     /gene="gppA"
                     /locus_tag="OQQ66_000749"
     CDS             35830..37314
                     /gene="gppA"
                     /locus_tag="OQQ66_000749"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1903771.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37361..39382)
                     /gene="rep"
                     /locus_tag="OQQ66_000750"
     CDS             complement(37361..39382)
                     /gene="rep"
                     /locus_tag="OQQ66_000750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1903772.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9864 a        10789 c        10046 g         9030 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg ttgcgggaga taaagtcggc atcattcgtc tggatgcctt taaaaccagt
      121 aaagagattc agtttgcggt gcagtcaggg ccaatgttat tagaaaacgg cgtaattaat
      181 ccacgcattc acccgaatgt tgcctcgcgt aaaattcgta acggtgtagg gattaataaa
      241 caagggaacg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ttgctttatc ttgatggcac aatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttaagc attgtacact cgaggaggcc agacaaactg ggactggcta
      481 cacagctgga aaatcatcag ataaatgaag ggaaacaaaa atgaaaaaaa cgctcgcaac
      541 gttattttta ttaacgtgtc tgggatcatc atccgcctat gcggataacg ctttgatttt
      601 acaaactgat tttagcctga aagatggtgc cgtctcggcg atgaaagggg tcgcattcgg
      661 cgttgaccat aatcttaaaa tctttgattt aacacacgaa attccaccgt ataacatctg
      721 ggaaggcgct tatcgcctgt atcagactgc cagttactgg ccgcaaggtt cggtgtttgt
      781 tagcgtggtc gatccgggcg taggcactga ccgtaaatcg gtagtgctga aaaccaaaag
      841 cggtcaatac ttcgtttcac cagataacgg tacgctcact ttggtggcac aaactctggg
      901 tattgatagc gtccgggaaa tcgacgaaaa agctaaccgc ctgaaaggtt ctgaaaagtc
      961 atataccttc cacggtcgtg acgtgtatgc ctacaccggt gcacgtctgg cttccggggc
     1021 gattacgttc gaacaggtgg ggccagagct tccagcaaaa gttgtcgaac tttcttatca
     1081 gaaagcaaaa gcaacaaaag gtgaagtgaa gggcaatatc ccgattctcg atattcagta
     1141 cggcaacgtc tggagcaata tcagcgacga gttgctgaat caggcagaaa tcaaactcaa
     1201 cgacacgttg tgtgtgacca tttccgaagg ttcccagcaa aaatacgcag ggaaaatgcc
     1261 gtatgtcgca agcttcggcg atgtgccgga aggccagccg atggtgtatc tgaacagctt
     1321 attgaatgtt tccgtggcgc tgaatatgga taatttcgcg cagaaacatc aggttgcgtc
     1381 tggtgctgac tggaatattg atgtgaagaa gtgcgctaag taaggtgctg atgcggaatg
     1441 cggtgtggcg caccgcatcc cgcaacgtca caccgtctcc aaccgggcat aagccgccac
     1501 cagccattta ataccctggc cctggaatgc cacctgcaaa cggctatgtt caccgctgcc
     1561 ttccatattg acgatggtgc cttcaccaaa cttagcgtgg cgtacacgct ggccgagctt
     1621 gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctga ccgggcggct
     1681 taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcagctcgc caataaagcg
     1741 cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt aggtcagcgt
     1801 cagtttctgc atcgcgcggg ttacgccaac gtaggccaga cggcgttctt cttccagacg
     1861 cccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac cgacgataaa
     1921 cacctgcggg aactccagac ctttcgccga gtgcatggtc atcagttgta ccgcatcctg
     1981 ccaggtatcc gcctgccctt cgcctgcttc cagcgccgca tgggatagga acgcctgcag
     2041 cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg tcaccagttc
     2101 ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt acatggtacg
     2161 caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat cggcagtttc
     2221 ctgcgctaag gcgtcgatca gttccataaa ccgctgtaag gcgctggcag cacgtccggc
     2281 gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact ggcgatcgcg
     2341 cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg gcgtattcac
     2401 cacacgctca aaggccgcgt cgtcgttacg gttggcaatc aggcgcagat acgagagcgc
     2461 atctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt acggcatact
     2521 cgcctgcaac agtgcttctt ccagcacacg cgactgggcg ttactgcggt agagaatggc
     2581 gcactcggca agcgccccgc cgttgtcctg ccaggttttg atgcggttaa ccacaaaacg
     2641 cgcttcatcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt ccgcgccatc
     2701 ggtccacagt tttttaccca gacgcccgtt attgttttca atcagggcgt tagcggcgct
     2761 cagaatattg ctggtagagc ggtagttttg ctccagacga atagtttcgg caccggggaa
     2821 atcattaagg aaacgctgaa tattctccac ctgcgccccg cgccagccgt agattgactg
     2881 gtcgtcatca ccgacgatca tcactttgcc ggtgtcgccc gccagcaggc ggatccacgc
     2941 gtactgaatg ttgttggtat cctggaattc gtccaccagg atattggtaa aacgttcgcg
     3001 gtagtgttgc aggatatgcg gcttgttaag ccacaactcg tgagcgcgca gcagcagctc
     3061 ggcgaagtcc accaggcccg cgcggtcaca cgcttcctga tacgcctgat acaccttctg
     3121 ccaggtctgc tccaccggat taccgtagct ttgaatatga tgtggacgca ggccttcatc
     3181 tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct cgtcgaggtt
     3241 catcgccttg attaggcgtt tgagcaggcg tagctggtcc tcgctgtcga ggatctgaaa
     3301 atcctgcggc agattggcgt ccatatggtg cgcacgcagc agacggtgcg ccagcccgtg
     3361 gaaggtgccg acccacatgc cgccctggct ggtgcccatt agctgcccga tacgatgacg
     3421 catctccgcc gccgctttgt tggtaaacgt caccgccata atcgagtatg gcaagcagtt
     3481 ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac cactgcccgc
     3541 gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct gtttgtcatt
     3601 aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat aggttgggcg
     3661 gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttgc tgattatatc
     3721 agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg gctgtcccag
     3781 gtttgcatca gatcgccatt ttccggtctg atccaacagg cctgcattcc gctgcgaatt
     3841 gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc gatcggcacg
     3901 ttgagttttt ccgcagccag aaagtacata tcgctgaacg gttttgagcg cccgtgcggg
     3961 ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg ctgggcgtta
     4021 ccgttggtga tcgccaccag cggccatttc ttcgccagct gttttaaggt gtcgtgagtt
     4081 tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt tgatcattgc tgcgtgtgcg
     4141 cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc aatcgaacga
     4201 aaacgccagc gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac cgcctggcgc
     4261 aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt ttgcacaaag
     4321 gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag ggtatcatcc
     4381 aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat tatttccccc
     4441 gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa tcaagatgag
     4501 tatagatttg cgtggtggag aggttggcat gacccagtag ctcctgcaca ccacgaagat
     4561 cgccgctcga ctccagcata tgcgtagcga acgagtgacg taatttatgt gggtgaacgt
     4621 gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca ttacgcgcgg
     4681 agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca aacaggtcgc
     4741 gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc aggcggcgct
     4801 ctttgctgcc tttccccatt acccacactt caccagactc caggtcgagg tgtttgatat
     4861 ccagccccac cagctcggaa agacgcagac cagcgccgta catcacttcc agcattgcac
     4921 ggtcgcgtac agcgagggga tcgttgatat caatatccag cagccgattc atatcgtcga
     4981 cgtcgatatt tttcggcaga tgacgcggcg ctttcggtgc cgaaacacct ttcgccgggt
     5041 tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc gcagaaagcc
     5101 gtaacgccag acttgctgct cccagccctt tacggcgact gcgtacagca aaattgcgca
     5161 ccatcgtcac atcacattgc tgccagcttt gcaggccgtt ttcgctggca aaattgatga
     5221 tcgcctcaag ctgacgctgg tagttaagca gggttatcgg gctaagctgg cgctccacgc
     5281 tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc gttcaatcca
     5341 acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg ttccttgccc
     5401 ttgttgatag tgactggcat cgcgactggt aaacagcacg acacccaaat cagcatcgct
     5461 tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca ccaccagcag
     5521 ctctggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct gaatacgcag
     5581 cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg cacctaagcg
     5641 ccagcgatcc ggaaacaggc gcagactcgc acctgccagg ccgagatcgc gcgcccagcg
     5701 gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc gctgcaggta
     5761 gagtaggcga taaaacaggc cttcgttggc gatagcctgt tccatcaaca gcgccatgtt
     5821 ctcttccaga acatgaatat gattacgtgc gcgggccatg tgccactcga ccaacgaaac
     5881 ggtgccgcgt accggatgtg gcacacgtat cgcttctact gcgcgcgcat tacggataaa
     5941 aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct ccgtgagtgt
     6001 ttcctgcagt tcttccccta gttgcttcat agatgaataa atccgtcgta gacatgtacc
     6061 gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc aagacgaccg
     6121 ccggggagtt ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc tactgcaacc
     6181 gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc ataaacgcgt
     6241 aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg ctccggaaaa
     6301 cgctcgtggc tttccagaac aggaccaagc gtttctaccg ccgcggtatc gacatcatcg
     6361 acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat tgtctgctcg
     6421 gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac agcggaaggt
     6481 tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac ggtcagaacc
     6541 atccgcccgt tggcggtgct gacgcggata tcgcgcttat tggtcagccc tttcaggcgc
     6601 acaaaacggg caaagcagcg cgcaccgttg ccgcactgcg ccacttcact gccatcagca
     6661 ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac caccagcagc
     6721 tggtcaaacc ctacccccag gtgccgatcc gccagccgac gaatcagctc cggtgaaaaa
     6781 aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc atgcattttc
     6841 gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta gtaattcacc
     6901 tgggatggac catcccagtg gcgcgatcgt ttttatccgg caccgtggat tgcgtttgcg
     6961 tctctaccgg tttggtcggc ggcggtgcat ttttatctgc aggcgggaaa tagagcggac
     7021 ctttcagacc gcagcccgtc aggctgaaga gagtaagtaa tacagtgagt accttaaaca
     7081 cgtttttcat tatggattgc ctgtaaagtt catcgctttc tgctttctat catcgcagga
     7141 gaaggcgtaa aagcaagcgt ttagcgactc tttatgaagt ctgaaaagtg aggaaatgct
     7201 gcgagtataa cgcttactcg tgaggattta tgttctgctg gcagtttcgt gatcggcacc
     7261 tcgcgccagg atggctttat gcactatcgc aatgtggttt gtttgttggc ttttttagtt
     7321 ccgtcagcgt gaggatgctc gctcggtaaa cctgatcata atgtttacca gaaacaggga
     7381 aaggcgtggt gtaactgcgc cctaatgaag agacaaatct ttcgccacac agagacatca
     7441 ttattccggt gacggatgaa tcatgccgga acgcagcagc agaggtgcaa actttgaagc
     7501 cctgacccca tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga
     7561 cagtgaattt catcgcctgg ctgatcaact gtggctgacc attgaagagc atctggacga
     7621 ctgggatggc gacagcgata tcgactgcga aatcaacggc ggcgtgctga ccattacctt
     7681 tgaaaacggc agcaaaatca ttatcaaccg ccaggaaccg ctgcaccagg tatggctggc
     7741 aaccaaacaa ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgcag
     7801 cggcgaaacc ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt
     7861 cagtttccgc taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa
     7921 aatattgctg taatagcggc gtatcgtgat cctgattggc aggcggcatg ttaccgatag
     7981 attttgtgcg gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact
     8041 gcggcaggtt gaagttgatg aagcttgagc cgtaggtgaa gcggtcgtgc gatgacgagt
     8101 agaagcgact gacgtcacgc accagttcct ctttgctgcc ttcgcagtgg tgatacacct
     8161 caacccggtt gctttcgtca agaatgtaga tattaaagcc attctcgtct tgcgtttctt
     8221 cgaagaagaa ctggatgatc ccttcactag caaagccgtc caccaccgcc ggtaatttga
     8281 cgtgattggt ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atagcgccat
     8341 aaaactcgat ggcgttctcc agcttctgta ccgatacatt caggcgttcg aagaacaagc
     8401 cccaggtttg accagaaacg cgcagcgcct tgaaacgccc ggtttcctgg cgggtgctgg
     8461 aaaggcgcag ttcaatacac tcagaaacca gttgctgcac gcgagtacgg attaacccgc
     8521 gcagatgctg gctataacag aagacttcca cgctatctgg cggtgcggcg tcctgatgca
     8581 ttttgccgag aatagttttc agggcttcga tcatcgattg ctcgccgttg aagtgcagcg
     8641 tacgcacttc gttccacgag ttgcggtaca gcaggtcaac gctacctacc aggcaatttt
     8701 gattctcgcc aaagctgaag acatccagct tacggaaatc aaaatgcacc acctgattgc
     8761 ggaacgccgc tgtcgggtca tattccaggt taacgataat cgccagatgg cggatctcac
     8821 acgggctgta gagcgccttc ggtgtcggtg caggtaagcg cagcgggaaa tggtgcgaca
     8881 cgtcggcgac catctcctgc aacttaggca aatcgacaat gccgttacct ttaatataca
     8941 aacgggtgcg cgaggtcagc aggccgttaa accatgccca cgccaccagt ttgttcaggt
     9001 aacggttata ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat
     9061 acagatacca acctgaacgg ttagcacggc ccggcggcac ataaataaag gtcagattcg
     9121 gttccgagag atcgggtgaa atctgcgggt ttaccagcgt cactttacct ggtaatgctt
     9181 caaacgcggc atacagctta cgcgtcagca cgccgatatc ctgcggactg gcggagacgc
     9241 taaggttatt gcgacgcgca aagcggatca gattacggta gctctgcatc atcgcgtcga
     9301 gcaactcgtt gtgcgcctca cgcacctgat caatcttcca gttagcgcgg ttatcgagca
     9361 ttgccagacg agcttcgtcc caaccccact cgctcactaa ctggctcaac actgcgcgac
     9421 gccagcctac gcaggcgcgt tcacggctga gcttttcgca cacttttaaa tagaagcagc
     9481 gacgtactaa atccagacgg gtaaaatctt caatcgccgt caggtattca gtaacacgct
     9541 ccagcatcat gcagtatgga tcgagaccaa acgatacaat ctcaccgtcg tgcaaacgct
     9601 gtttgatatc tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca
     9661 gcagtgtttt cagtaccgct ttgtatgggg aatcgatact cttgtagagc tgccaaaggc
     9721 tggcaccaaa gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat
     9781 ttggcgtcag cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgctcttctt
     9841 cgtcgcacgg caccatattc cacagaatac gcttaccggc gagacgcacg gcggtacgat
     9901 aaaattcgtc aagcagcagt atatgctggg tggagccaca atcttcgccc cccaagctgc
     9961 cgctttcatt atgacggaag cggttttcat caatcaggaa gaagctgact tccacaccca
    10021 gcgaggcggc ccagctttcc agcagactac atttacgttg tagtaactgg cgctcttcgc
    10081 tatcgagcca ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta
    10141 cggacgaggt gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggtggat
    10201 cctgtactga cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat
    10261 caggcgtgta aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg
    10321 gatggtgata gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca
    10381 tagcagcaag cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag
    10441 tctcaatata gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc
    10501 gccaacatca acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta
    10561 ccacttttcg cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca
    10621 atttaacacc ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt
    10681 ttattctctg taaattcctt attacgacgg cgtgaaacgc ctgtcaggat ccactgccag
    10741 acctcatttt acggtttgcg caggcgtcta cgtttcacca caacactgac atcactctgg
    10801 caaggatgtt aggatggacc acggatgata atgacggtaa caagcatgtt agacaatgtt
    10861 ttaagaattg ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac
    10921 aagttgatgg cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc
    10981 gatgtgattc ttgatacgcc gctggcgaaa gtaggcggaa aaggcttatt tgtaaaagag
    11041 ctggaagtcg cgctcctcga aaatcgcgcc gatatcgccg tacattcaat gaaagatgtg
    11101 ccggttgaat tcccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc
    11161 gatgcctttg tgtccaataa ctatgacagt ctggatgcgt taccagcagg cagtatcgtc
    11221 gggacgtcca gtttacgtcg ccagtgccaa ctggctgaac gccgcccgga tctgattatc
    11281 cgctccctgc gcggcaacgt cggcactcgc ctgagcaaac tagataacgg cgaatacgat
    11341 gccatcattc ttgccgtagc cggactaaaa cgtttaggtc tggagtcccg cattcgcgcc
    11401 gcgttaccac ccgagatttc tcttccagcg gtaggacaag gtgcggtcgg tattgaatgc
    11461 cgccttgatg atgcacgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca
    11521 ctgcgcgtta ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtgcca
    11581 attggtagct acgccgagct tattgatggt gaaatctggc tgcgtgcgct ggtcggcgcg
    11641 ccggacggtt cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaga tgccgaacaa
    11701 atggggattt cgctggcaga agagctactg aataacggcg cgcgcgagat cctcgctgaa
    11761 gtctataacg gagacgcccc ggcatgagta tcctggtcac ccgcccgtct cccgctggag
    11821 aagagttagt gagccgtctg cgcacactgg ggcaggtggc ctggcatttt ccgctgattg
    11881 agttttctcc gggtcgacaa ttaccgcaac ttgctgatca actggcggcg ctgggggaga
    11941 gcgatctgtt gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctgcatc
    12001 agcaagatcg taaatggccc cgactacctg attatttcgc cattggacgc accaccgcac
    12061 tggcgctaca taccgtaagc ggacagaaga ttctctaccc gcaggatcgg gaaatcagcg
    12121 aagtcttgct acaattacct gaattacaaa atattgcggg caaacgtgcg ctgatattac
    12181 gtggcaatgg cggtcgtgag ctaattgggg ataccctgac agcgcgcggt gctgaggtca
    12241 ctttttgtga atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc
    12301 gctggcaatc ccgcgaggtg acgatggtcg ttgttaccag cggtgaaatg ttgcagcaac
    12361 tctggtcgct gatcccacaa tggtatcgtg agcactggtt actacgctgt cgactattgg
    12421 tcgtcagtga gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaaggtcg
    12481 ccgataacgc tgacaacgat gcgcttttac gggcattaca ataactctca taacaggaag
    12541 ccataatgac ggaacaagaa aaaacctccg ccgtggttga agagaccagg gaggccgtgg
    12601 acaccacgtc acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc
    12661 tcagcgcggt ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta
    12721 aacaacaggc cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat
    12781 tgcaaaaagc ccaggagagc caaaaagccg agctggaagg cattattaag caacaagctg
    12841 cacagcttaa gcaggcgaat cgtcagcaag aaacgctggc aaaacagttg gatgaagtcc
    12901 aacaaaaggt cgccaccatt tccggcagcg atgctaaaac ctggctgctg gctcaggccg
    12961 attttctggt gaaactcgcc ggacggaagc tgtggagcga tcaggacgtc acgaccgctg
    13021 cagcgttgct gaaaagtgca gatgccagcc tggcggatat gaatgacccg agtctgatta
    13081 ccgttcgtcg ggcaattacc gatgatatcg ccagcctttc tgcagtttcg caggtggatt
    13141 atgacggcat catccttaag cttaatcagc tttcaaatca ggtagataac ctgcgtctgg
    13201 ccgataatga cagcgatggt tcgccgatgg attccgatgg tgaagagctt tccagttcca
    13261 tcagcgaatg gcgtatcaat ctgcaaaaaa gctggcagaa ctttatggac aacttcatta
    13321 cgattcgccg tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc
    13381 tgcgcgaaaa tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcaccagg
    13441 aagagactta tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata
    13501 ctgatgatgc caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata
    13561 tctcgatgga tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga
    13621 ctcgcgtgcg taacctgctg gcacaaccgg cagcagggac aacggaagct aaacctgcac
    13681 ctgcacctgc acctgcacct gcaccgcaag gtgatactcc ggcagccgcg ccgcaaggag
    13741 aataaccatg ctaaaagtgt tattactctt tgttttgctg attgcgggga tagtggttgg
    13801 cccgatgatt gccggtcatc agggttatgt gctgatccag accgacaact acaatatcga
    13861 aaccagcgtc acgggcctgg cgatcatatt gattctggcg atggtagtgc tgtttgccat
    13921 tgagtggcta ctgcggcgga tcttccgcac tggcgcgcac acccgtgggt ggtttgtcgg
    13981 gcgtaagcgt cgccgtgcac gtaagcagac cgaacaggcg ctgctgaaac tggcggaagg
    14041 cgattatcag caagttgaaa agctgatggc gaaaaatgcc gatcacgctg aacaaccggt
    14101 ggtgaactat ctactggctg ccgaagccgc gcaacaacgt ggtgatgaag cgcgcgccaa
    14161 ccaacatctg gaacgcgcag cggagctggc cggcaatgac accattccgg tagaaatcac
    14221 ccgtgtacgt ctgcaactgg cccgtaatga aaaccatgct gcacgccacg gcgtggataa
    14281 gctgctggaa gttacgccac gccatccgga agtattacgt ctggcggaac aggcgtatat
    14341 ccgcacaggt gcatggagtt ctctgctgga tatcatccca tcaatggcga aagcccatgt
    14401 tggtgatgaa gaacatcgtg caatgctgga acaacaggca tggattggcc tgatggatca
    14461 ggcgcgtgcc gataacggta gtgaaggttt gcgtaactgg tggaaaaacc aaagccggaa
    14521 aacgcgtcat caagtagcgt tgcaggtggc aatggcggaa catcttattg aatgtgacga
    14581 tcatgatact gcccagcaaa ttatcatcga tggcctgaaa cgccagtacg acgatcgcct
    14641 actgctgccg attcctcgac tgaaaaccaa caatccggaa cagctggaaa aagtgctgcg
    14701 ccagcagatc aaaaacgtcg gcgatcgccc gctgttgtgg agcacactgg gccagtcact
    14761 gatgaagcac ggagaatggc aggaagcatc gctcgccttc cgcgcagcgc tgaaacaacg
    14821 tccggacgcc tacgattacg catggcttgc cgacgcgctg gacagactgc acaagccgga
    14881 agaagctgca gcgatgcgtc gcgacggttt gatgttaacg ttgcagaaca acccaccaca
    14941 gtagttcctt ctcacccgga ggcaagcacc tccgggacct ttctgatgca taaaaaaacg
    15001 cctgctctta ttacggagca ggcgttaaaa caggtctgta tgacaacaag tgggtgcttc
    15061 actcaacgtt gtgtccatgg tgtctgatga ggcataagcg acatctgtca gtggacgata
    15121 agcaccgtaa acggtgctgc gtcattccgg agtttatgag gcactaaggc gaacataaga
    15181 gatggaatga gcatctactc gtttattatg ccacagagaa tcgggaaata acatccctta
    15241 acacttgtta tgagataatt ctgtaatcct ctttgcttcc tgagtaataa cttcctgagt
    15301 gaatatttaa cctgagcttg atcctacaca tacttattat gaatgataaa attcattcaa
    15361 ttaataacac atatattaat tgccgttaaa actaaaaaaa gcatcaataa tcaacgcgat
    15421 ataataaacc tgccttacat atcaactgcg ccagaggtag gattgaaaac gctctcctga
    15481 ttttccaatt cattttctgg ataaataaat aatttatttt tgtcactatt atttatgtaa
    15541 tcatcctgtc agggagaggg atctcaatta tcaatgctta attacgtcat cattttgatg
    15601 acatgaaacc ggagcatttt gcatggaatt ttcgttttca cccaaacttc ttgttgttgc
    15661 tgtcgccgcc gctcttcctc tcatggccag cgcagcagat accccgtcaa ctgccaccgc
    15721 acgcaaaggc tttgccggat acgatcaccc aaaccagtat ccacagaacg ctgcaattta
    15781 ttgaatttgc acgatcatgt aggccggata aggcgtttac gccgcatccg gcaatcaacc
    15841 gcaggcggcc acaaaatggc cgctatttta cttattcacc accagcaaat gtgcctgcat
    15901 aatgtcactg gccgggcgac cgtgcgccag caaatcagcc attgctttaa gatatggcgg
    15961 tagatggcgg aaataacgct gatacccggc acacaaataa ttcagccccg gtttgccgct
    16021 ggcatcgagc atgaagcggt gtttcgggca gcctccccag cacgctttta acacgttaca
    16081 actgcgacac tgcgccggta attgcttaaa tttatcttca ccaaacacct gctgttgcgg
    16141 ggaatcgatc atttctgcaa ttgtttgctg atgcatattc cccagccgat attgcggata
    16201 aacatagtga tcgcaggcgt aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca
    16261 ggttggctga tgatggcaaa ccgcacccgg cgcaccgaca aaattggcaa acgcccattc
    16321 gatattcatc acgaaaatct tgccgacgtc gcgtttgatc cagtggtcaa aaatcgccac
    16381 cagaaattca ccaaattcat cggggcgcac agaccattcc gtcagttccc cctgaatatc
    16441 accaggcgca tgtagtttca gtccatcgct ggcagctgtt tcatcagcca ggcgctcgac
    16501 caccggaata aactggatga attcgactcc cgcatcgcac aaaaaatcat aaacctgcaa
    16561 cggttgctgc gcgctggtgc ggttgacgca gaccagcacg ttatagtcga catgatgttt
    16621 ttgcagcagc gtcagggcac gcatcaccag cttatgcgtt ggtctgccac ctttggtcac
    16681 gcgatattga ttgtggatct cagccggacc gtccagcgat aacccaacaa gaaaatgatt
    16741 ttctgccaga aatgcacacc atttatcatc gagcagcacg ccgttagtct ggaagctgtt
    16801 acttatcttc ctgccagcac cgtatttcgc ctgtaacttt acggcacggc ggtaaaaatc
    16861 cagcccgagt aacgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc
    16921 gctggcagcg atatagtgac ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac
    16981 tggcttttcg cggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt
    17041 acaatcggaa ccactcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgcag
    17101 catggtcact ccttgcctga agtcatcctt caatacgtta agggcgaaat cataccgtgg
    17161 ggcaatgagt tattacgggc gtttattgcg aagagagggt gacgaaatgc acagaaaaca
    17221 aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg aacctccgac
    17281 ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt
    17341 gaatttttag ttcaattatt taaagtcgtg gtgcgagggg ggggacttga acccccacgt
    17401 ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt
    17461 cacaacttct aataatgggg tggctaatgg gattcgaacc cacgacaact ggaatcacaa
    17521 tccagggctc taccaactga gctatagcca ccactacaaa tcttgttacg cggtattact
    17581 accaccgcag ctcaagcgcc gggactaaat ggcgcgcccg acaggattcg aacctgagac
    17641 ctctgcctcc ggagggcagc gctctatcca gctgagctac gggcgcttag cgccgttgcg
    17701 ggggtggata atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg
    17761 tttggttacg gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttcccgtggc
    17821 gattaaggcc aaaaacttta taaatcgccg tcaccgccag cataaagatg atgccaacaa
    17881 acagcgacat acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg
    17941 ccattgttac gtagttggcc cacgggaaca ggattgagcg gaacggatgg ctggcaatcg
    18001 ccgctttatg tgcacgccga aaacgcagct ggcttatcaa tatcacaaac catggcacca
    18061 tccccggaag cacgctggca ctgtagacgt agacaaacac acgctgcgga ttgggaatga
    18121 tgtagttcag gcatgagcca attagcagaa tagcaataga tactgccaca cccgcaactg
    18181 gtacgccgtg acgggaaact ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg
    18241 cgtagagcat acgtccgcaa ctgtacatgc cgctgttaca gccagagagc gcagccgtca
    18301 gcaccacaaa gttgataatg cccgccgctg cggtaatacc gattttggca aaagtcagta
    18361 cgaacgggct gccgttgctg ccactttcat tccacgggaa gatggtgacg ataacgaaaa
    18421 tcgcgcctac gtagaaaatc aggatccgcc acagcacttt gcctacggca ctgcgcaacg
    18481 tcacctgcgg attcttcgct tcaccggcag taatgccaat cagctccacg ccctggtagg
    18541 acgccaccac aatacacagc gcggtcagga accctttcca gccacccgca aagaaaccgc
    18601 catgctctgt gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa
    18661 tcacgcccag gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc
    18721 agaactcgat ttcgccgtac aaccgcaccg ccgccagatt cgccaacgcc accagcgcca
    18781 ctgcgatcaa tgcgggtatc cactgcgcca tctccgggaa ccagaactgg acataaacgc
    18841 caacggcggt gatttctgag atccccaccg ccatccacat aaaccaataa gaccaggcgg
    18901 tgagataacc aaagaacggg ctcatataac gatgcgcata aacggcgaac gaaccggtaa
    18961 ccggttcgag gaacaacatt tcgcccattg aacgcatgat gaaaaagacg aatagcccgg
    19021 cgatgatata ggccaacaat acggatggcc cggcccattt caaggtactg gcggccccca
    19081 taaacaggcc gacgccaatg gtgcccccca gggcgatgag ttcgatatgt cgagcttcca
    19141 gcccacgctg tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct
    19201 ttccggtgtt accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc
    19261 aggagaaatg gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac
    19321 aggcgacgga gtgaccactc cgtcgcttta caaagagagg aaaatcagag gttgccggtg
    19381 tagtgccagc gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa
    19441 agcaggcggt agagccactc cagtcccagc gtttgccaga ttttcggtgc gcgttttacg
    19501 tgaccggtga aaacatcgta agtcccgcca acgcccatat acagcgcatc tggatgcacc
    19561 agacggcagt cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt
    19621 tgcgctccgc tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa
    19681 ccatcctgac tgccaacgat attcacattc cactggttgc gcagtttagc ttcagtttgc
    19741 gccagcactt caggtttacc gcccacaaga aataccggcg tcccttcttt gcctgcgcgc
    19801 gccatcagct cttcccagag atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac
    19861 tttttacgta ctgaacgtac aacgctgatg ccatccgcat atttaaattc ggcagcgtta
    19921 attaactccc tgacctcggc gttatcttca atggtcagca ttttttcagc attaatggca
    19981 accagcgttc cctgcttaag ctgcccgtca gcaaacagat aatcgagggc gtgctgcata
    20041 tcacgccaac caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta
    20101 ttcattgtta tccttcaacc tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc
    20161 cggcgctttc aaaaagccag tacaacagtt ttgcgatcat cagacatgcg ccgaagacca
    20221 cgataaaaaa gactacgcgt gagacaaacg aatccagccc ttcacgtgcc agcacgatca
    20281 tattgaagat cgccccaaag cagaaactgt gcaatatcgc agccttatag cgattaggct
    20341 cgcggttgcc cagctcatac agccagtcga accatttgat gatcagacca accacgatcg
    20401 ccccgagcgg gatgaacaac gcgccgccca tcaccaccag tgagcctata agcgtaggcg
    20461 agatcgccag tccggagtgg ttattcagca cttccctggt aaagtagttg gctgagttca
    20521 gcaccatact cgggcgaccc ggccacagcc aggaagggat aaagacatag aaatcgcgga
    20581 caattggagc caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat
    20641 tctcccacgg cgagaaggtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc
    20701 cgctcacatt cattccatag cgttttagcg ccagccagaa catgcccaca atccccagca
    20761 cgcccgccgc cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga
    20821 agatagcgaa tgcgatgatg atattggcgc gagtgccgcc gacaatcata taagtcagca
    20881 agccaaaggc gaccgtgctg acgaggaaaa acagccacgc tttgctatcc tggcgcagaa
    20941 agtagaccac cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg
    21001 agacttcact ggagaagatc tgactgtagg agttaagccg gaacagcaaa aagccgttgt
    21061 gcatgaagaa gatgccgacg cttaccagcg cgatgcccat caggatcacc cacgtaagat
    21121 tggtctccac gcggttcatg gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc
    21181 gtaggcgggt tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg
    21241 cctgcaacaa gatttctggc ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg
    21301 tcagcgggaa gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt
    21361 taaagcgcac acggcgaaac tcaaaccaag tcagcgtggc aataaacagc gtgcagagca
    21421 gccagaccac aaacaggcca ctgaattgca gcagactcat gcgacctccc cggcggcaat
    21481 cgccaacacc cgctgccagc cttgtagata gttagggcta aagaaggcaa tggtgttttt
    21541 atccaccgac gccaactggc gctgcgcttc acgcacaata tcctcgttga gatcgtcggt
    21601 agtaaacagc accggcaaat gctgttccgt catatcctgc cagaacggat tttcccggtt
    21661 aagcacacaa ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc cctgctggcg
    21721 ggcaaaaata aagtaaccaa gatcgcactg acgaagtagc gccagatagg cgtcaaattc
    21781 cagtttttcg ctcaaaattt gcagattttc ttcgctgaat aactccagcc ccgcctgacg
    21841 aacttcctca atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac
    21901 cgtatcgcca aattgctgat gaacggcgcg caaggcagca atatggtcat tgctgcggtc
    21961 accggagttc cccaccagaa tggtcatttt cccttcacgt tgccgatcgt tcgccatcgt
    22021 attgagagaa gggtccatcc gcgtcgggaa gtacagcagt tcgccccgca cctttggatg
    22081 cgttttggca aaaaagctca aatcaccgcg ggtggcaaat acacagccga ctcgcttttg
    22141 cgccaggcga cgtagcgggt aaaaaagctt atatctcaag ccactggaaa gctcatacag
    22201 gtctgccccc cagatatgcc agaaaaactg gctgggctta atgccaccac tcagcagagc
    22261 cagccacagt gtgggattga actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt
    22321 tgctttcgcg atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag
    22381 cgccggacag ctatcactta agccgtcgtc cttgccaaca accataaact cgcgcgcgtg
    22441 ctcgctcgtc gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg
    22501 gatatccgat cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac
    22561 acgccacaac aaagagaaaa atagacgata taagttgcca tatatgcctg cgccgcgccc
    22621 agtgcaccat gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg
    22681 acttccgcca gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca
    22741 taagcgccca cttttagcac atcacccact aactgccagg caaagagatc gcgcatagcg
    22801 gtaaatttat tcgacaacag caaccagata gcaaaatcac gcagcaacca gacggtaaaa
    22861 ctcgccgccg ccactgccgg taagacgaat tttagcgatt taaccacttc ccgggtgata
    22921 tcgcgctttt ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta
    22981 atgaattgca ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacttcatcc
    23041 cagctatact gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc
    23101 gacgtaatca acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag
    23161 ctgggtttca gatagcttaa cgggatgaca ccacgtttga tcaacatgat ggcggcagga
    23221 attaccacca gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa
    23281 cggtaactga cgtagtacgc gagaacgcca atcaagctgc cgacaatcag cgataacgca
    23341 ttacctgcgg catcgcgaaa gcctttcatc agcgccagta acaggttgcc ccaggcgatc
    23401 cccatttgca ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacaat
    23461 ccctggctga ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt agagaaacca
    23521 agtaccatcg ctgatgaagt gccgaccacg cggcgcagct gttgcggatt atcatggtac
    23581 tgggcaacgt atttggttac accgttaaag atgccagccc cggcaagcac gccgagcacg
    23641 gtaatcaact ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc
    23701 agcaacttac cgaccagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac
    23761 aaggacgctt ttgccaacga catatcagga gaagtagttc aacaaagtcg caattaccgt
    23821 acgctgattg acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc
    23881 tttggtggtg tagcgatctt caccgtggaa ctcaccaaag tgttcccccg cagggcaacc
    23941 gtgcagcgga atgtaatgaa acaccgccat gatttccgct tctttcagaa agttaatcaa
    24001 cgcgctccgg tcatcaatat cccgcagttt aatgtagaac atatgcgcgt tctgcacgca
    24061 gccatcagga atcgacggca gctcgatacg cccggctttc gccagaggcg ctaacgcatc
    24121 gtagtaattt tgccacagag ccagacgttg ctggttgata cgatccgctg cttccagttg
    24181 cgcccacagg tatgcagctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca
    24241 ggtatattta tcgacctgac cacggaagaa ctggctgcgg ttagtgccct tttcacggat
    24301 gatctcggct cgttcgatta acgctttatc gttaatcagc gtcgcgccgc cttcaccacc
    24361 cgccgtgtag tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag
    24421 tgcacgccct ttgtaagtgg acatcacgcc ctgagcggca tcttccacca caaacaaatt
    24481 atgctttttc gccaacgcca taatggtgtc catttcgcag gccacacccg cgtaatggac
    24541 cggcacgata acgcgcgttt tgtcggtgat cgctgcttca atcaacgttt cgtcgatgtt
    24601 catggtgtcc gggcgaacat ccacaaagac gattttcgcg ccacgcagca caaaggcatt
    24661 ggcggtggag acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag
    24721 cagcagcgcc gccatctcca gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct
    24781 gccaaaacgt tgctccagcc actgctggca gcgacgggta aaaccgccat cgccacacag
    24841 tttgccgcta cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc
    24901 gttaaatgga atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac
    24961 cactttgtat gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag
    25021 ttgttttacc gcgagcatac gcccagttta gcgccgtttg catcagctca gcacctgcac
    25081 cgcgtccagc cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat
    25141 agccgcgaat atcgccggac gccgcacgta gaatcagaca ttgatgatca aaggtgccgc
    25201 gcacggcatt ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg
    25261 gcgcacgaaa acggctttgc gcaaatgcgg cgctagctaa ctgacgtaat gcgggaatat
    25321 cggtctcttg tgccacgaca gcaccgctat cactgacatt gttcacgggt agcgccaaat
    25381 caacttcacc ttctaccagg gagaatccca gctgttgcag ggcatccagt tcacccgtat
    25441 ttgatgccgc aattttggcc tgcacccgtg accacggcgc taacgcgtct ggcgtcagga
    25501 gcggtgcttc agacgtaatg cgcacgatgg cgctgttaac accaaagaag gcgttttccc
    25561 aggttagtgg ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg
    25621 tagccagttt tcgctaatga actggcagca cgcttcacac cctcgtcatc gagccagccg
    25681 ttacgccagg cgatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc
    25741 tgcacaaagg tactggcttc aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat
    25801 ccgcgcccga gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag
    25861 gtaatctcca gttcaccacg ctccgacggc ttcacctgct ttgcgtactc cacgacttta
    25921 ctgtcgtaga aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct
    25981 tccagcgaga tagcgcggaa attgtcgtca aactccacca cgccaaagcg ttcctggtcc
    26041 atcacctgat agccaaatac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc
    26101 ggactgaagc cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcaccg
    26161 ttgaggaagg tttcaccgat gataaaggcc tgcaccagac cgtccgggct gggctgttcg
    26221 gcgtattcca gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct
    26281 ttatcttccg gcgtagtgat gatgagaatt tcgcggatac cggccaacat cagcaccgac
    26341 agcggatagt aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc
    26401 gtaatcggat gcaatcgggt gccggaaccg cccgccagga taataccttt catgtttgcc
    26461 tccgctgaaa attagccttt cagacctaaa cgctcgccct gatagctgcc gtcctgcacc
    26521 tgcttccacc agctttcatt agccagatac cactgcaccg ttttacgcat tccactttca
    26581 aaggtttcct gtggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa
    26641 cgcagatcat gccccggacg gtcagcgaca aaggtgatca agtcacgata gtgcaccacg
    26701 ccgtgcggct tgttcggagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg
    26761 agattcttac gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc
    26821 gccacgcaat acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc
    26881 ccgttgccat ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc
    26941 agtttttccg gaaagtggta aggaccgtag ttattcgagc agttggtgat aagcgtcggc
    27001 agaccgtagg tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa
    27061 taagggctgc tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg
    27121 tcaccatata cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc
    27181 gtcagcgtat tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg
    27241 gtttcaataa acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga
    27301 tgcatgacac agtctggctg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag
    27361 atatcaactt tctcaaaggc aaagcgatcg ctttgcgcga ccggtgccag cgacatcagg
    27421 tttccggcgt agatcagctt atcgaccact accaccgcgt cgctcgtttc gttgatgata
    27481 taacgcacca gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc
    27541 agcgccagac tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa
    27601 ctttaaactg actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt
    27661 caagctgcgc cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct
    27721 caacaaccag agtttcgccg ctatgccact gggcgatcag ttcagcgatt tccatcgccg
    27781 ggctttcgcg caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca
    27841 gttcactggc gcgtttatcg gtagccgcca ggcaatcagc caccgccgct ttcacctgat
    27901 cgataaccca gaacggtttg tgatcgttca cttcgcgcgc ggtacggata agccgcgcct
    27961 gctgggggtt ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc
    28021 cagggccagg ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc
    28081 agacgttaat cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca
    28141 catcgcggaa gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga
    28201 cacactcacc ttcgaggaaa attttgtaca gttcgctggc gcgggtcgaa caaaccggcg
    28261 tcataccacc aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaaca
    28321 cgcgttccgg gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag
    28381 tgagatccgg acgcatctct gctaaccatt ctgccatctt ctcggttgac cccaccggcg
    28441 aggtggattc aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag
    28501 ccgattcaac gtaggtcata tctggctcat gatcgccttt aaacggcgtg ggtacagcaa
    28561 tcagccaggc atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg
    28621 cagtttttac tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg
    28681 tatcaaccgc gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg
    28741 ctgctgctgt aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca
    28801 tagtgatatc cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc
    28861 atacgggtta tgggcgcggc tcatagtttg atattcgttt tcgtctttta aaagacgcgt
    28921 cacttcctcg acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac
    28981 cgcttccgga cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc
    29041 ttcttcctga atgccgcctg agtcggtcaa aatcagccag gcgtggttca tcaaccagac
    29101 aaacggtaaa tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg
    29161 attgaccggc tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg
    29221 gtgcgtggtg gcaatgtctg ccaacgcgtg gcagatttct tcaaaaccac gaccgaaact
    29281 ctcacgcctg tgaccggtca ccagaatcat ctttttattg gggtcgataa acgggtaatt
    29341 tgccaccagt tctgaacgca gcgtgtcgct gctcatcacc tgatcacgca cccataacag
    29401 tgcatcaatg actgtattac cagtaatgaa gattcggcta tccgcaacgt tttcacgcag
    29461 caagttttgc cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa
    29521 tgtacggtta gcctcttccg gccacggcga atagagatcg cccgtgcgta aaccagcctc
    29581 aacgtgacca acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgttgt
    29641 tgtgtcgccg tgaaccagca cgacgtctgg tttgaactcg gcaagaatag gttttagccc
    29701 ttccagaatc cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc
    29761 gtagtcaggt acaatggaaa agagtttcag cacctgatcg agcatctccc gatgctgcgc
    29821 agtgacgcaa actttagcct caaaaaaagg agcttttgcc aacgcatgca ccaacggagc
    29881 catcttgatg gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt
    29941 cgaataagcg gcgagcgcct ttgcgctcgc cgcagcagtg ttgctatttc gagcaacggc
    30001 gggttaatgc gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga
    30061 aggcacgacg tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg
    30121 tctgaaaacg cggatccagg gttggaccaa cattcagggt gtttaacatg gcccgattct
    30181 gatcatagtc gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga
    30241 gcattggacg cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg
    30301 tcgcactgcg cgaaatatta tgctgctcag caattttcag cgcctgctca atgctgttca
    30361 tccggcggtc gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct
    30421 ggatggtgcg tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct
    30481 ggctggcaaa agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa
    30541 taagcttcac gctgtcattg accgcgcggg taaagtctcc ggggataaac tggatgttgt
    30601 taatcatttc atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt
    30661 aatagtcggt ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa
    30721 actctttata ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga
    30781 catccaggtt acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg
    30841 gacgatcggt aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg
    30901 cgattaacgc aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac
    30961 gaaacaaccc acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg
    31021 gttgtgtcat cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg
    31081 cagtctgcgt tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata
    31141 tccatagagg aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg
    31201 agaatattct gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac
    31261 aaacgcctga cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc
    31321 aggagagaat gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat
    31381 tagcggaatg gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg
    31441 ggtcgtttcg agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc
    31501 cataaagact ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat
    31561 ggcggcgatc atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat
    31621 cataccgatt gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc
    31681 aaccatgttg aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa
    31741 cggtccgagc gccatctccc aggagccaaa gatataaccc aggctactga gataaagttt
    31801 gccgaacacc atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct
    31861 gatatcaaaa cggtcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata
    31921 gagagatgca tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta
    31981 aaccgaaatc cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt
    32041 atccactaaa ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa
    32101 taaaaaaata ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag
    32161 agaaaattat tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc
    32221 cgaacccacc agtcctgtgc aaaaattaac catttgatat gcaaattgca taacatgcca
    32281 gcaaattcca ggaattttcc taacatcgcc agcgcggcat taagattaca gatataaaag
    32341 caaaacgcca cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc
    32401 atttcgaaga aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt
    32461 gcatcgattt cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct
    32521 tcctgagtcg tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg
    32581 aagacgcgtt tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta
    32641 aactcttcgt aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata
    32701 atggtcaggc tgccgccctc ttccacgtta cgcgccgcac cgaagaagcg tttcggacga
    32761 tgaagggcat tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg
    32821 ttgtaagcgc gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc
    32881 tcaaccaggc gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg
    32941 ggttcgtcaa aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact
    33001 tcttccggac gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca
    33061 atgctctgag caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc
    33121 agaccacgct gaccacgacc gataggtgat gccagatcca gtacgcgagc ggttaaatct
    33181 tccgtagaac cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag
    33241 ttctcaaaga ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact
    33301 ttcagcagcg caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta
    33361 tcaccagtgc ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga
    33421 ccggcgaggt aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc
    33481 agtacgccat caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa
    33541 ataatgtcct gcttacgcat acgagccagg ttttccagcc ccatattttc gccgagagtg
    33601 atcagctcag aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt
    33661 aaacttgggg taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct
    33721 gtttacggtc atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg
    33781 aaacgacaag acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa
    33841 acgggaagcg ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca
    33901 acactaagtt agcatgactc acgccgggcg tccagttttt aacgacgggg cacccgaaca
    33961 tgaaattccc ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg
    34021 cacccacttt ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac
    34081 ggatgccata tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca
    34141 gtttgccctg atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac
    34201 cgcaccactc tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt
    34261 caaaactgtc gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa
    34321 gcctggcgtg ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt
    34381 caccggatac gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa
    34441 cacatttaac agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagtcc
    34501 ttgaaaaaaa agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc
    34561 tggcgggtcg tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc
    34621 ttacgtcaac gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc
    34681 aaccacgtgc cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg
    34741 cagaaccgct ggcggaagct accggcctga agctgggtct ggcttacggt ggtgatggct
    34801 acgacaaaca gctgaaagtg ctggaaagcg gcgttgacat tctgattggc actacggggc
    34861 gtttaattga ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtggtggtac
    34921 tggacgaagc cgatcgcatg tacgatctgg gctttattaa agatatccgc tggctgttcc
    34981 gccgtatgcc gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc
    35041 gggtacgtga actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg
    35101 aacagaaaac gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa
    35161 tgcgtttgct gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca
    35221 ataccaaaca ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg
    35281 gtttgttgac aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc
    35341 gtggcgatct ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg
    35401 cagtgacgca cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta
    35461 ttggtcgtac aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt
    35521 atgcattgaa tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat
    35581 acaatccgga cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccacgta
    35641 caggcaatgg tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa
    35701 aatgtcatac tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga
    35761 gcatactgta gtaagtgatt caggtgaaag aatgcagcca acacagagac agattgaagg
    35821 atgaagagta tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt
    35881 tttcatatgc tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaataaaa
    35941 cgcaaagtgc gtctggctgc tggcctgaat agcgaaaatg ccctgtctaa tgaagcaatg
    36001 gagcgcggtt ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg
    36061 caaattcgcg ttgtcgctac ggcgacatta cgtcttgccg tcaatgcggg tgattttatt
    36121 gccaaagcgc aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagca
    36181 cgtctgattt atcagggcgt tgctcacaca actggcggtg ccgatcagcg cctggtggtg
    36241 gatataggcg gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg
    36301 ttcagcctgt cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg
    36361 gggcaggaaa attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc
    36421 gatgaattac ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg
    36481 ttacaggaaa tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag
    36541 caattgaaac agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg
    36601 acgctggaac gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc
    36661 gaactgaata ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac
    36721 ggcatgttgc atctggccgt cgagcaggat attcgcagcc gtacgctgcg taatattcag
    36781 cgccgcttta tgatcgatat tgatcaggcg cagcgcgtag ccaaagttgc ggctaacttc
    36841 ttcgatcagg tggaaaagga atggcatctt gaagcaataa gccgcgattt gctcatcagc
    36901 gcctgtcagc ttcatgaaat cggcctgagc gttgacttta aacaagcgcc gcaacacgct
    36961 gcttatctgg tgcgtaatct ggatcttccc ggttttaccc ccgcacagaa aaaattgctg
    37021 gcgacgctac tgctcaacca gactaatccg gtcgatctct catcgctgca tcagcaaaat
    37081 gccgtaccgc cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc catcattttt
    37141 gccagccgtc gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg
    37201 ttgaccttga cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt
    37261 gctcaggaaa gccagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta
    37321 agtgccggat gcgatgctga cgcatctttt ccggccttga ttatttccct cgttttgccg
    37381 ccatcatcgc tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcgc
    37441 tgaccacttt gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc
    37501 ggctcggctc cgggcgcacc agctcgccgt actgacggcg ttctttacac agcgtaaagg
    37561 tcaattcctt ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat
    37621 tatcttcatc gatgctgctc tggtgcggca aaaacccttc ttccataccg accatgtaga
    37681 cataaggaaa ctccagccct tttgacgcgt gcagagtcat cagttgcacc tgatccagct
    37741 cttcttcact ctcaccacgc tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg
    37801 tgagcgtcat cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca
    37861 gttggttgac gttcttcatg cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca
    37921 gccaggattc ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct
    37981 ccgccagacg ctggatttct gccaaccagt gagtaaagcg ggtcaatgct tcataaccac
    38041 gtccgctaag cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc
    38101 gcgtcatcgc ccactcaccc agctttttca gcgtcgccgg gccaatctct ctcttcggcg
    38161 tgttaacgat acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag
    38221 ccagcaagtc cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg
    38281 ggatgcggtt ttgcatcagg aatttttcaa acactcgcga ctgatggtta ccgcgataaa
    38341 gaatagcgta atatttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag
    38401 tgacgcgctc ggcttcatgt tcttcgttat tcgcgcttaa aactttaagc tctgtgccgt
    38461 aacccagttc ggagaacaga cgcttctcaa agacgtgcgg gttattggcg atcaggatgt
    38521 tcgccgcttt caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca
    38581 gcgccggaaa atcctgactc agcagcacca ggttttgtgg acgtgcgccg cgccaggagt
    38641 agatcgactg gtcgtcatcg cccaccacgg taaagcgcgc gcggctgccc accagcagtt
    38701 tcaccagttc atactggctg gtgttggtgt cctgatactc atccaccagc agatagcgaa
    38761 ttttgttctg ccagcgctca cggacttctt cattgcgttg cagcagcaac gtcggcaata
    38821 aaatcagatc atcgaagtcg agaacattgc acgctttcag gtgtgcatca tacagcccat
    38881 aacaatgggc aaaaatacgg tcccgctcac caatcgcact ggctgccgcc tgggacggtg
    38941 ttttgagatc gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt
    39001 catcttcaat cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa
    39061 acagcgaaaa gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc
    39121 ccagcgtgtg gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc
    39181 cgacacgctc tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc
    39241 gcgcctgata accgcaaccg cggatcagat gggcgatttt attggtgatc acacgagttt
    39301 taccggaacc cgcgcccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt
    39361 gttggccggg gtttagacgc ataggtgtat tgctcaatct tcgaacgggg ggaggattgt
    39421 agcagaatcg catttgaatg tgaccgcgtg taagccgcta accggttggc tgcggatgtt
    39481 taaatcagag agggaagacc gaaaccggag ttcatcgtga atccattaga cttagaaaat
    39541 atcgggtcgt aacgttgtgt tattactttt tcagagttta ctaaggagag gtgtactcgt
    39601 ggcgattaat ttcagtccta aggttggtga aatactggaa tgcaactttg gtaactatcc
    39661 tgttagccag aatggacagt tcagcaccac gtattacgat ggccgcattc ctcctgaaat
    39721 gataaaaaa
//
LOCUS       DAJVTY010000015        39338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVTY010000015 DAJVTY010000000
VERSION     DAJVTY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39338
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.015"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="OQQ66_000751"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="OQQ66_000751"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1903773.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OQQ66_000752"
                     /pseudo
     CDS             complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OQQ66_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2717..2907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3021..4121
                     /gene="trmA"
                     /locus_tag="OQQ66_000753"
     CDS             3021..4121
                     /gene="trmA"
                     /locus_tag="OQQ66_000753"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1903774.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4161..4520)
                     /locus_tag="OQQ66_000754"
     CDS             complement(4161..4520)
                     /locus_tag="OQQ66_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1903775.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OQQ66_000755"
     CDS             complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OQQ66_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1903776.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5491..6891
                     /gene="sthA"
                     /locus_tag="OQQ66_000756"
     CDS             5491..6891
                     /gene="sthA"
                     /locus_tag="OQQ66_000756"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1903777.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OQQ66_000757"
     CDS             complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OQQ66_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1903778.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8058..9431)
                     /gene="argH"
                     /locus_tag="OQQ66_000758"
     CDS             complement(8058..9431)
                     /gene="argH"
                     /locus_tag="OQQ66_000758"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1903779.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9492..10268)
                     /gene="argB"
                     /locus_tag="OQQ66_000759"
     CDS             complement(9492..10268)
                     /gene="argB"
                     /locus_tag="OQQ66_000759"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1903780.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10276..11280)
                     /gene="argC"
                     /locus_tag="OQQ66_000760"
     CDS             complement(10276..11280)
                     /gene="argC"
                     /locus_tag="OQQ66_000760"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1903781.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11434..12585
                     /gene="argE"
                     /locus_tag="OQQ66_000761"
     CDS             11434..12585
                     /gene="argE"
                     /locus_tag="OQQ66_000761"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1903782.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGHECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12937..15588
                     /gene="ppc"
                     /locus_tag="OQQ66_000762"
     CDS             12937..15588
                     /gene="ppc"
                     /locus_tag="OQQ66_000762"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1903783.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIR
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15771..17504
                     /locus_tag="OQQ66_000763"
     CDS             15771..17504
                     /locus_tag="OQQ66_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1903784.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17719..18528
                     /locus_tag="OQQ66_000764"
     CDS             17719..18528
                     /locus_tag="OQQ66_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1903785.1"
                     /translation="MYHDISYLLSRLINGPVPDLAYQVDFPRLEIVLEGEFVDTGAGA
                     TLVPGDVLYVAAGGWNFPQWKTPTTTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVAR
                     RGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQIQTASRSQALFEAI
                     RDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKG
                     YDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE"
     gene            complement(18515..18856)
                     /locus_tag="OQQ66_000765"
     CDS             complement(18515..18856)
                     /locus_tag="OQQ66_000765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1903786.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18858..19736)
                     /locus_tag="OQQ66_000766"
     CDS             complement(18858..19736)
                     /locus_tag="OQQ66_000766"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1903787.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19702..21999)
                     /locus_tag="OQQ66_000767"
     CDS             complement(19702..21999)
                     /locus_tag="OQQ66_000767"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1903788.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22050..22370)
                     /locus_tag="OQQ66_000768"
     CDS             complement(22050..22370)
                     /locus_tag="OQQ66_000768"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1903789.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22385..23464)
                     /locus_tag="OQQ66_000769"
     CDS             complement(22385..23464)
                     /locus_tag="OQQ66_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1903790.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23773..26274
                     /gene="ptsP"
                     /locus_tag="OQQ66_000770"
     CDS             23773..26274
                     /gene="ptsP"
                     /locus_tag="OQQ66_000770"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1903791.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26286..26948
                     /gene="fsa"
                     /locus_tag="OQQ66_000771"
     CDS             26286..26948
                     /gene="fsa"
                     /locus_tag="OQQ66_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1903792.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            26959..28062
                     /gene="gldA"
                     /locus_tag="OQQ66_000772"
     CDS             26959..28062
                     /gene="gldA"
                     /locus_tag="OQQ66_000772"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS1903793.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28336..28953
                     /locus_tag="OQQ66_000773"
     CDS             28336..28953
                     /locus_tag="OQQ66_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1903794.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(28980..29885)
                     /gene="yijE"
                     /locus_tag="OQQ66_000774"
     CDS             complement(28980..29885)
                     /gene="yijE"
                     /locus_tag="OQQ66_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1903795.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(29979..32159)
                     /gene="katG"
                     /locus_tag="OQQ66_000775"
     CDS             complement(29979..32159)
                     /gene="katG"
                     /locus_tag="OQQ66_000775"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1903796.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32488..33378)
                     /gene="metF"
                     /locus_tag="OQQ66_000776"
     CDS             complement(32488..33378)
                     /gene="metF"
                     /locus_tag="OQQ66_000776"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1903797.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33727..36159)
                     /gene="metL"
                     /locus_tag="OQQ66_000777"
     CDS             complement(33727..36159)
                     /gene="metL"
                     /locus_tag="OQQ66_000777"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1903798.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36162..37322)
                     /gene="metB"
                     /locus_tag="OQQ66_000778"
     CDS             complement(36162..37322)
                     /gene="metB"
                     /locus_tag="OQQ66_000778"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1903799.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37599..37916
                     /gene="metJ"
                     /locus_tag="OQQ66_000779"
     CDS             37599..37916
                     /gene="metJ"
                     /locus_tag="OQQ66_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1903800.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38100..38708
                     /locus_tag="OQQ66_000780"
     CDS             38100..38708
                     /locus_tag="OQQ66_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1903801.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38853..39338
                     /locus_tag="OQQ66_000781"
                     /pseudo
     CDS             38853..39338
                     /locus_tag="OQQ66_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         9638 a        10886 c         9886 g         8928 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacta gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccaccactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acggatatcg agcaacggtg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaacatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcacgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2341 gccagattca ggcgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2401 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2461 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2521 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2581 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2641 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2701 ttcatcaata ttacgcgatg atgagacgtt ggccggcagg tcttcgggct tggaggggta
     2761 tctaagatac taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca
     2821 tcgcaccttt ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact
     2881 cacaggaccg gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac
     2941 aaataggaat tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact
     3001 ttaatttttc aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc
     3061 agttagccga aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc
     3121 cggaagtgtt tcgctcgccg gtcagtcatt accggatgcg tgcggagttc cgcatctggc
     3181 acgatggcga tgacctgtat cacatcattt tcgatcaaca aaccaaaagc cgtatccgcg
     3241 tggatagctt ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg
     3301 gtgtgcgtaa taatcccgtt ctgcgccaca agttgttcca gattgattac ctcaccacgc
     3361 tgagtaatca ggcggtggtt tccctgctat accataagaa gctggatgat gagtggcgtc
     3421 aggaagcgga ggccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg
     3481 gtcgggcaac gaaaaccaaa atcgagctgg atcaagatta catcgacgag cgtctgccgg
     3541 tcgcagggaa agagatgatc taccgtcagg tagaaaacag ctttacccag ccgaacgcgg
     3601 cgatgaatat tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt
     3661 tactggagct gtactgcggc aacggtaact tttcattagc gctggcgcgc aattttgatc
     3721 gggtattagc caccgaaatc gctaagccgt cggttgctgc tgcgcaatac aacatcgcag
     3781 ctaaccatat tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga
     3841 tgaatggtgt gcgcgagttt aaccgcctgc aagggatcga cttaaagagt tatcagtgcg
     3901 aaaccatttt tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc
     3961 aggcgtatcc gtgtattttg tacatctcct gtaacccgga aacgttatgc aagaatctgg
     4021 aaacattaag ccagacgcac aaggtcgaac gtctggctct gtttgatcag ttcccctaca
     4081 cgcaccatat ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgcc
     4141 agcatccggc ataataccga ttactcagca acttcctgct tacggttacg catcttcgcg
     4201 ccaatccaga acaccataat gacggagagt actgccggga agaagttaga gccgatatcc
     4261 ggatattccg cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaggcagct
     4321 gccagacccg gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa
     4381 accgtcagca ccagggaaat aatcgggaat acagaaaatg gcacaatgga gctaaacagc
     4441 gctgcgaaag taccattaat cgataagcca gcaactaacg ccagcagcag cgtacctcta
     4501 tcttgatttg cttgtttcat tactcgtcct tcacatttcc cggaataatt gcggttttct
     4561 cttgttcacg gcgataccag taataagccc ctttcgaaat cattcgcagt tgcagtacca
     4621 gtcgctcttc taattgccga cgttgttcga cgccgacgtc caacgcctcg gcacccgcac
     4681 tgaagacaat tgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt
     4741 tttcgagttc cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg
     4801 cggcacgaaa cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag
     4861 gattattacc gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt
     4921 tggcgatacg ctgacgcgcc tggcgcatga gttggcgtag cattaaaccg ctctcatcaa
     4981 ccatggtcag acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa
     5041 tgcccgcttc acgcgccact tcacgcaaac tcaggctggc gaagctgcgt tcagcactta
     5101 attggctaaa tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta
     5161 tgcccatcac gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga
     5221 acatactgga ttgctccagt attgtgaatg aacggtaacg caataaaaca tatttactgg
     5281 ccggttcttt tacgtacagc ggaaacctgc cgcttaaacg gagagtatcg tcgataaaaa
     5341 tccaataaaa cgtcagggca aaagtaagaa acagacaaag caaaggccgc tcaggatata
     5401 gccagataaa tgacggggat caattggctt acccgcgata aaatgttacc attctgttgc
     5461 ttttatgtat aagaacaggt aagccctacc atgccacatt cctacgatta cgatgccata
     5521 gtaataggtt ccggccccgg cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg
     5581 cgcgtcgcag ttatcgagcg ttatcaaaat gttggcggcg gttgcaccca ctggggcacc
     5641 atcccgtcga aagctctccg tcacgccgtc agccgcatta tagaattcaa tcaaaaccca
     5701 ctatacagcg accattcccg actgctccgc tcttcttttg ccgatatcct taaccatgcc
     5761 gataacgtga ttaatcaaca aacgcgcatg cgtcagggat tttacgaacg taatcactgt
     5821 gaaatattgc aggggaacgc tcgctttgtt gacgagcata cgttggtgct ggattgcccg
     5881 gacggcagcg tcgaaacact aaccgctgaa aaatttgtta ttgcctgcgg ctctcgtcca
     5941 tatcatccaa cagatgttga tttcacccat ccacgcattt acgacagcga ctcaattctt
     6001 agcatgcacc acgaaccgcg ccatgtactt atctatggtg ctggagtgat cggctgtgaa
     6061 tatgcgtcga tcttccgcgg tatggatgta aaagtggatc tgatcaacac ccgcgatcgc
     6121 ctgctggcat ttctcgatca agagatgtca gattctctct cctatcactt ctggaacagt
     6181 ggcgtagtga ttcgtcacaa cgaagagtac gagaagatcg aaggctgtga cgatggtgtg
     6241 atcatgcatt tgaagtcggg taaaaaactg aaagctgact gcctgctcta tgccaacggt
     6301 cgcaccggta ataccgattc gctggcgtta cagaacattg ggctggaaac tgacagccgc
     6361 ggacagctga aggtcaacag catgtatcag accgcacagc cgcacgttta cgcggtgggc
     6421 gacgtgattg gttatccgag cctggcatca gcggcctatg accaggggcg cattgccgcg
     6481 caggcgctgg taaaaggcga agccaccgca catctgattg aagatatccc taccggcatt
     6541 tacaccatcc cggaaatcag ctctgtgggc aaaaccgaac agcagctgac cgcaatgaaa
     6601 gtgccatatg aagtgggccg cgcccagttt aaacatctgg cacgcgcaca aatcgtcggc
     6661 atgaacgtgg gcacgctgaa aattttgttc catcgggaaa caaaagagat tctgggtatt
     6721 cactgctttg gcgagcgcgc tgccgaaatt attcatatcg gtcaggcgat tatggaacag
     6781 aaaggtggcg gcaacactat tgagtacttc gtcaacacca cctttaacta cccgacgatg
     6841 gcagaagcct atcgggtagc tgcgctaaat ggcttaaacc gcctgtttta acactttatc
     6901 gaaatggcca tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag
     6961 cggtgagcca ggacgataaa ccaggccaat cgtgcggcga ggttccggct taatacacgg
     7021 caaataaaca accccatcgc gtttgcgctc cggcggcaca gccagcgcag gcagtaaagt
     7081 gatcccgcta cctgccgcca ccatgttacg cagtgtctcc aggctggtcg cgcggaagtg
     7141 tgtatcttca tccgccccgg cttcaaagca gaaacccatt gcctgatcgc gcaaacagtg
     7201 accatcttcc agcatcagca gtttttcccc tgccagatcg gccatcggta cgcattcgcg
     7261 gttcgcccac gggtgatctt catagatagc cagcaacatt ggctcatcaa acaacggcac
     7321 ttcaatgaat gcttcgctct ctttcaccag cgcgaggatc acgcaatcga gtttgccgct
     7381 gtccagttgc gccagtaact ggtgggtctg tgcttcatgc agatacattt ccagctttgg
     7441 aaatgtctgg tgcagcatcg ggataatatg cggcagcagg tacggtccaa ccgtaggaat
     7501 caaaccaata tgcagcggtc cggacatcgt ctcgccctgc tggcttgcca tctctttaag
     7561 gactttcacc tcacgcagca cggtacgcgc ctgatccacc agcagcattc ccgcctgggt
     7621 gaacaacact ttacggctgg tccgctccag caacatcacg cccagctcat cttccagctt
     7681 acgaatttgg ccgctaagcg tcggctggct aacgtggcag gaatctgccg cacgccgaaa
     7741 atggcggtgt tcagccaatg ccaccaggta ctcaagatca cgaatattca ttatccatcc
     7801 tccatcgcca cgatagttca tggcgatagg tagaatagca atgaacgatt atccctatca
     7861 agcattctga ctgataattg ctcacagaaa cggagcggca cctcttttaa cccttgaagt
     7921 cactgcccgt ttcgagagtt tctcaactcg aataactaaa gccaacgtga acttttgcgg
     7981 atctccagga tccgcttttt tttgccataa aaaagcccgg cgataagcca ggctcaaatt
     8041 tgtacatata aatgttctta ccccaaccgc gcctgcgcaa aagcaatcgc ttgggccacc
     8101 tgctgcggtg agacgccgcc ttttgccgca cgcttgtcga ggcacgattg tagcgacaga
     8161 atcggataga catcttcgcc aatcacgtgg ctgaatttct gcaactcgtc gagcggcaga
     8221 tcttccagcg gtttgccctg acgaatggct tccaccaccg cttcacccac gatatggtgc
     8281 gcctcgcgga aaggtacgcc tttcgccacc aggtaatccg ccagttcggt ggcgttggcg
     8341 taaccctgct gagccgcttc ctggcaacgt ggacgtttca cctgaatgcc gtccagcacc
     8401 agcgccgcca tatgcaggca gtccagccag gtatcgaacg cgtcgaacag accttctttg
     8461 tcttcctgca tatctttgtt gtaagccagc ggcaaacctt tcagcgtcat catcatgccg
     8521 gttaacgcac cctgcacccg accgcattta ccgcgaatca gctccagcgc atccgggttt
     8581 ttcttctgcg gcattaatga tgaaccggaa gtcacgcggt cagaaagctc cacaaacccc
     8641 gcttcgccgg tgttaaagaa aatcagatct tcagcaaaac gcgacagatg caccatgccg
     8701 atagcggcag ccgacagcag ttccaacaca tggtcacggt cagaaacgct gtccagactg
     8761 ttacgggtcg ccgaagcaaa gcccagccag cctgctaact gttcacggtc gatttcatag
     8821 gccgttcccg ccagcgcgcc acagcctaat gggctgacat ccagacgctt aagcgcatcc
     8881 tgcaaacggc tttcatcacg cgccagcatc tcaacatagg ccaggcacca gtgcgcgaac
     8941 gtcaccggct gggcgcgttg caggtgagtg taacctggca ttaccgcgtc ctgattgttt
     9001 tgtgcggttt ccaccagcgc cgattgcagc tgccggttag ccgtcagtaa ctcgctaacg
     9061 gtatctttgc accacagttt caggtcagtc gctacctgat cattacggct acgcccggta
     9121 tgcagctttt tgcctaactg gcccactttg tcgatcagtt tgccttccac ccagctatgg
     9181 atatcttcgg cgtcgctttc aaggatttgt tgtggcctgg cgcgaacatc ttccagcaac
     9241 acgttcagcg cctcttccag ttgcgcctgc tcttctgcgg ttaacacgcc gaccgttacc
     9301 agggctttgg accaggccac agagccaaca atatcctgct ccgccagacg gtaatcaaag
     9361 cgcagtgagt cgttgaattg tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc
     9421 caaagtgcca taactctgtt tccttatttt gaaattcaat gccggaaagc tgcgcctccg
     9481 gctaacaaaa cttaagctaa aatccgcgta cccatcggca taccgttaaa cagtgccgga
     9541 agctgctccg catgacgcca ggaggcgata tctaccggac ggcccagcgt gcgggccgca
     9601 tccagcgccg cgttcacttt cactatcatg ccgtcagtaa taatgccctg ctcaatcagt
     9661 tgttctgctt tcgcggcggt catttcggca atgcgttgcc ctttgccgtc gagaatgccg
     9721 ctgacgtcgg agagcaaaat cagatccgcg cccagcgttg ccgccagcgc cgttgccgcc
     9781 tggtcggcat tgacgttcat cagttgccct tcgtctgtta cgccaatgga gctgaccacc
     9841 ggcagataac cgttctccag caaggagttg ataagcttag gcgaacctgg ctgcgccagt
     9901 ccaacatgac ctaactcttc atcaagctgg gtcactttga cgctgtcgcc atcaccgaga
     9961 aacaaaccta cagccgcaat ctgatgtttc ttcgcccatg ccaacagggt tttatttgcc
    10021 gttcccgcca gtgctccggt gataatgtct atctgatcag caggcgtcac ccgcaggccg
    10081 tttttctttt tcaccggcag attcagccct ttcatcagct catccaccac gcaaccgccg
    10141 ccgtgcacaa tcaccagcgg acgctgatgt gactcacgat aattcaccag tgcgctaaac
    10201 agacgttcca gcgcctcttc actatccagc agtacgccgc ccagtttgat aattaatgga
    10261 ttcatcattg cacccttaaa taagagactg cgtttccgca tagccgaaac gaatattggc
    10321 gcactgtacc gcttgtgccg ccgcgccttt cagtaagttg tcttcggtcg ccacaataat
    10381 cagatgctcg ccctgaacgg caaacccgat atcgcaaaat ggcagcccaa cgacattttt
    10441 cagcgccgga acgcctttgt catacagccg caccagcggt ttatgggcat acgcctgctg
    10501 taacacttgc gcgacttgcg cctgggtcac acccgatttc aggcggcagg taatggtttc
    10561 gagaatgccg cgcgggaaat tgcccagatg tggggtgaag ataacgtcag caccgaggtg
    10621 tgtggcgatc tctggttgat ggcgatgagt aaagacgcca tacggttgca ggctaacttc
    10681 acaaaaacta tttgaaatcg ctgccttacg tcctgcaccg ctcacgccgc tggtggcgtt
    10741 aatcgccggc cactgattga ggtcaagaag atcggcatca atcaacggtt tcagcgccag
    10801 ctgtgccgcc gtcggataac agcctggcac cgcaatcaaa ttcgcttctt ttaatttatt
    10861 accgcaccac tccgccagac cgtaggctgc ctgttccagc agttccgggt attgatgggt
    10921 aaagccgtaa tatttttcat agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc
    10981 gaacaccacg cagcccgctt caagaaactg cggcgctaaa tcgtggctaa cttcatgggc
    11041 ggtggcgaga aacactacgt ccacccctgg gctaaactcg ctgatatccg acatcggctg
    11101 caacggcagc tcaacgatgc cttttagctg cggatgcaaa tcggagatta actttcccgc
    11161 atcattgctt tgcgctgaaa cagtcaaagc ggttatgttc atatgcggat ggcgatttac
    11221 ataggtcact agctctgcgc cagcgtagcc gctggcaccc acaatcagcg tattcaacat
    11281 cggggctatt caccttctta tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg
    11341 gctggtgggt tttattacgc tcaacgttag tgtattttta ttcataaata ctgcatgaat
    11401 attgatacta tcatgaccag aggtgtgtca acaatgaaaa acaaattacc gccatttatc
    11461 gagatttacc gcgctctgat tgccacacct tcaataagcg ctacggaaga ggcactcgat
    11521 caaagcaatg cagatttaat cactctgctg gcggactggt ttaaagattt gggcttcaat
    11581 gtggaagtgc agcctgttcc aggaactcgc aacaaattca atatgctggc aagtatcgga
    11641 cagggggctg gcggcttgtt gctggcgggg cataccgata cggtgccatt tgatgacggt
    11701 cgctggacgc gcgatccgtt tacactgacg gagcatgacg gcaagcttta cggcttaggc
    11761 accgccgaca tgaaaggctt ttttgcgttt atccttgatg cgctacgcga tatcgacgtc
    11821 acgaaactga aaaaaccgct ctacattctg gcgactgctg atgaagaaac cagtatggcc
    11881 ggagcgcgtt attttgccga aactaccgcc ctgcgcccgg attgcgccat cattggcgaa
    11941 ccgacgtcac tacaaccggt acgcgcacat aaaggtcata tctctaacgc catccgtatt
    12001 cagggccagt cggggcactc cagcgatcca gcacgcggag ttaacgctat cgaactaatg
    12061 cacgacgcca tcgggcatat tttgcaattg cgcgataacc tgaaagaacg ttatcactac
    12121 gaagcattta ccgtgccata ccctacgctc aacctcgggc atattcacgg tggcgacgct
    12181 tctaaccgta tttgcgcttg ctgtgagttg catatggata ttcgtccgct gcctggcatg
    12241 acactcaatg aacttaatgg tttgctcaac gatgcattgg ctccggtgat cgaacgctgg
    12301 ccgggtcgtc tgacggtcga cgagctgcat ccgccgatcc ctggccatga atgcccaccg
    12361 aatcatcaac tggttgaagt ggttgagaaa ttgctcggag caaaaaccga agtggtgaat
    12421 tactgtaccg aagcgccgtt tattcaaacg ttatgcccga cgctggtgct cggtccgggt
    12481 tccatcaatc aagctcatca gcctgatgaa tatctggaga cgcggtttat taaacccacc
    12541 cgtgagttga ttatccaggt aattcaccat ttttgctggc attaaaacgt aggccggata
    12601 aggcgctcgc gccgcatccg gcaccgttgc caaactccgg tgccgcaata atgtcggatg
    12661 cgatgcttgc gcatcttatc cgacctacac ctttggtgtt atgtatggcg attttttaac
    12721 atttccataa gttacgctta tttaaagcgt cgtgaattta atggcgtaaa ttcctgctat
    12781 ttattcgttt gctgaagcga tttcgcagca tttgacgtca ccgcttttac gtggctttat
    12841 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
    12901 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
    12961 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
    13021 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt ccaaatcttc acgcgctggc
    13081 aatgatgcta accgccagga gttactcacc accttacaaa atttgtcgaa cgacgagctg
    13141 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac
    13201 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
    13261 cgtaaactga aaaaccagcc ggaactgagc gaagacacca tcaaaaaagc agtggaatcg
    13321 ctgtcgctgg aactggtcct cacggctcac cctaccgaaa ttacccgtcg tacactgatc
    13381 cacaaaatgg tggaagtgaa cgcctgctta aaacagctcg ataacaaaga tatcgctgac
    13441 tacgaacaca accagctgat gcgtcgcctg cgccagttaa tcgcccagtc atggcatacc
    13501 gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag ccaaatgggg ctttgccgta
    13561 gtggaaaaca gcctgtggca aggcgtacca aattacctgc gcgaactgaa cgaacaactg
    13621 gaagagaacc tcggctacaa actgcccgtc gaatttgttc cggtccgttt tacttcgtgg
    13681 atgggcggcg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
    13741 ctactcagcc gctggaaagc caccgatttg ttcctgaaag atattcaggt gctggtttct
    13801 gaactgtcga tggttgaagc gccccctgaa ctgctggcgc tggttggcga agaaggtgcc
    13861 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
    13921 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaaggcct gctgacacaa
    13981 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
    14041 ggtattatcg ccaacggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
    14101 ccgctggtcc gtattgatat ccgtcaggag agcacccgcc ataccgaagc gctgggcgag
    14161 ctgacccgct acctcggtat cggcgactac aaaagctggt cagaggccga caaacaggcg
    14221 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc
    14281 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc
    14341 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtactggc tgtccacctg
    14401 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
    14461 gatgacctga acaacgccaa cgatgtcatg acccagctgc tcaatatcga ctggtatcgc
    14521 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
    14581 ggcgtgatgg cagcgtcctg ggcgcaatat caggcacagg atgcattaat cagaacctgc
    14641 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
    14701 ggtgcacctg cccatgcggc actgctgtca caaccgccag gaagcctgaa aggcggcctg
    14761 cgcgtaaccg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
    14821 agcagcctgt cgctttatac cggggcgatt ctggaagcca acctgctgcc accgccggag
    14881 ccgaaagaga gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatgtctac
    14941 cgcggctacg tacgtgaaaa caaagatttt gtgccttact tgcgctccgc tacgccggaa
    15001 caagaactgg gcaaactgcc gttgggttca cgtccggcga aacgtcgccc aaccggcggc
    15061 gtcgagtcac tacgcgccat tccgtggatc ttcgcctgga cgcaaaaccg tctgatgctc
    15121 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagacgg caaacagagc
    15181 gagctggagg ctatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
    15241 atggtcttcg ccaaagcaga cctgtggctg gcggaatact atgaccaacg cctggtagac
    15301 aaagcactgt ggccgttagg taaagagtta cgcaatctgc aagaagaaga catcaaagtg
    15361 gtgctggcga ttgccaacga ttcccatctg atggccgatc tgccgtggat tgcagagtct
    15421 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
    15481 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga acaagcgtta
    15541 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctcttct
    15601 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
    15661 tttcaatata tttctgtctg cattttattc aaattctgaa tataccttca gatatcctta
    15721 aggaattgtc gttacattcg gcgatatttt tttcaagaca ggttcttact atgcattcca
    15781 cagaagtcca ggctaaaccc ctttttagct ggaaagccct gggttgggca ctgctctact
    15841 tttggttttt ctctactctg ctacaggcca ttatttacat cagtggttat agtggcacca
    15901 acggcattcg cgactcgctg ttattcagct cgctgtggtt gatcccggta ttcctctttc
    15961 cgaagcggat taaaattatt gccgcagtaa tcggcgtggt gctatgggcg gcctctctgg
    16021 cggcgctatg ctactacgtc atctacgggc aggagttctc gcagagcgtt ctgtttgtga
    16081 tgttcgaaac caacaccaac gaagccagcg agtatttaag ccagtatttc agcctgaaaa
    16141 ttgtgcttat cgcgctggcc tatacggcgg tggcagttct gctgtggaca cgcctgcgcc
    16201 cggtctatat tccaaagccg tggcgttatg ttgtctcttt tgccctgctt tatggcttga
    16261 ttctgcatcc gatcgccatg aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg
    16321 ataacctggc ctcgcgtatg gagcctgccg caccgtggca attcctgacc ggctattatc
    16381 agtatcgtca gcaactaaac tcgctaacaa agttactgaa tgaaaataat gccttgccgc
    16441 cactggctaa tttcaaagat gaatcgggta acgaaccgcg cactttagtg ctggtgattg
    16501 gcgagtcgac ccagcgcgga cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg
    16561 agctggatgc gctgcataaa accgatccga atctgaccgt gtttaataac gtagttacgt
    16621 ctcgtccgta caccattgaa atcctgcaac aggcgctgac ctttgccaat gaaaagaacc
    16681 cggatctgta tctgacgcag ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa
    16741 ccttctggat caccaaccag cagacgatga ccgcccgcaa taccatgctg acggtatttt
    16801 cgcgccagac cgacaagcag tactacatga accagcaacg tacgcagagt gcgcgtgaat
    16861 acgacaccaa cgtgctgaag ccgttccagg atgtgctgaa tgaccctgcg ccgaagaaac
    16921 tgatcatcgt tcatctgctg ggtacgcata tcaaatacaa ataccgctac ccggaaaatc
    16981 agggcaagtt tgatggcaat accgatcatg ttccgccagg attaagcgca gaagagctgg
    17041 aatcatataa cgattatgac aacgctaact tgtataacga tcatgtggtt gccagcctga
    17101 ttaaagactt taaagcggca gacccgaacg gattcctggt ttacttctct gaccacggtg
    17161 aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc
    17221 gccacatgta caccattccg ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc
    17281 cccgtgattt ctcacaggat gtcgatcgta aatacagcct ggcggaactg atccacacct
    17341 ggtcagattt ggcgggctta tcttacgacg gttacgatcc aacccgttca gtggtgaatc
    17401 cgcagttcaa agaaactacc cgctggattg gtaacccgta caagaaaaac gcgctgatcg
    17461 attacgacac tctgccgtat ggcgaccagg taggtaatca gtaattatca gccaggcgct
    17521 atttgccgga tgcgacgctt gacgcgtctt atccggccta cagatcgcgg cggtaggctg
    17581 gataaggcgt ttacgccaca tccggcaata caactgccgc aatgtttaca tcgaactccc
    17641 ctcaggccac acttttcagg tggccttttt taataatgcc agttactgtt ttcccgtcta
    17701 catacctaag ggaataacat gtatcacgac atcagctatc tgctctcccg cctgatcaac
    17761 ggcccggtgc ccgatcttgc gtatcaggtc gattttccac ggctggaaat tgtgctggaa
    17821 ggtgagtttg ttgataccgg cgctggagca acgttagttc ccggcgatgt gctgtacgtt
    17881 gccgctggtg gctggaattt cccacaatgg aaaacaccca ctaccacctt tagcgtgctg
    17941 tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg atggcaaaca atatcaaaat
    18001 ctggcgaagc aacacgtcgc ccgacgcggc ccacgtatag gttcttttct gctacaaacg
    18061 ctcaatgaaa tgcaaatgca gccgcaggag cagcaaacgg caaggcttat cgtagccagc
    18121 ctgcttagcc actgccgcga tttgctcggc agccaaatcc agaccgcctc acgcagccag
    18181 gcactatttg aagctattcg cgattatatc gacgaacgct atgcctccgc gcttacccgc
    18241 gaatctgtcg cacaggcatt ctatatttca ccaaactatc tctcgcacct gtttcaaaaa
    18301 acgggggcca ttggttttaa cgaatacctg aatcacacgc gactggagca cgctaagacg
    18361 ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac
    18421 agcaactact tctgccgtct gtttcgtaaa aacaccgaac gctcgccgtc ggaatatcgc
    18481 cgacagtacc acagccaact gacagaaaaa ccgactactc cagaataaga tgggtttgct
    18541 gcggcgcaga aagcactttg cgcaccgcgc tcattacccg ctgcggctca cgcaggaatg
    18601 cgtagatgct gcattgcaca tagcggcaat gttcaaatcg ctcggcaccg gcaagctcga
    18661 tatccgtaat caacagagca acatccgccc gacggatatc ctcgtccgct aaacgattct
    18721 ccgttcccag cgcgccctga gtttcaatgc tgacgcccca cttctctaac tggcacaact
    18781 tttccagccg ttccgccgcc atataagtat gcgccacgcc gcttacgcag gcggttactg
    18841 ccaccaggta tgccatttta acctcccacg gtaacctgaa atccggcccg ttctgccatt
    18901 tcgcgcatcg ttgccacatc ggctgatgac ggcgcagcaa catccttcat cgaccatgtt
    18961 ttccccagca ggcggtattt cggctcgccg tactgatgaa acggcaacag atggatctgc
    19021 ctgatattca gcgggatcag cacgtccagc gcctgctgca tattctcccg gctgagtgtg
    19081 aaaccgggga tcagcggcaa acgcgggatc acgttgacac cctcactcac cagcaaacgc
    19141 aggttctcca gtacgcgtgg caggttcatc ttcaccacat cccgcgcctg agtcgcgtcc
    19201 ataattttta aatcgaacaa cacttcatcg cacaatttcg ccagcggtaa cagcttgctg
    19261 gctggtgcgt ctccggcagt ttcaatggca catgagacac cccacagccg cagtcgctgt
    19321 aaaaaacggg tagcaaactc cgcctgcatt aacacttcgc cgccagaaag cgtgacgccg
    19381 ccgccggacg tgcgaaaaaa aatgtcatct ttcatcactt cccgttccag agcgtcaagg
    19441 ctgatatcgc gaccaatccg ttcaaacgcc ccggaggggc attcatccgc atcacgcaaa
    19501 catttcgcac agtgcagaca tttcgcctct ctgcgtaccg tctggatttt gccggagatc
    19561 gactccggat tagcacacca cgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta
    19621 cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt tgaaaatgcg cgccacatca
    19681 ttgaggcgcg tttccacaac gttacagctg atgcgctgtc cggcggatga tgtcatcctg
    19741 gatctccttc gacagttcga caaagaaggc gctgtatccg gcaacgcgca ccaccagccc
    19801 ggcataatct tgtgggcgct gttgcgcttc ccacaacgtg tcggcgttca ccacgttaaa
    19861 ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag aagtcggcca gtttgcgtaa
    19921 tcccgcttca ccttccaggg tcgccggagt gaatttcacg ttcagcaacg taccgttaga
    19981 cagcagcgta ttatcgagct tactgactga cttcagtacc gctgttggcc cttgttcgtc
    20041 ctggcccagc ataggtgaca agccgccgtc tgccagctgt tctccggcaa aacgaccgtc
    20101 tggcgtcgcg ccaaccaccg atcccaacgg gacgtgagca gaaacggtat acgatcccgg
    20161 cgtgaagtaa ccaccgcgcg ggttctggta tttttccact tctttgcagt agtggcgcaa
    20221 cagttcggcg ctaatgttat ccacctcgtc gatatcgtta ccatatttct caaagcggtt
    20281 aattaagcga gcgcggactt tttcgccttc tggcgttgca aagttggctt ttaataccga
    20341 cagcaattcg tcaaaactta aacgctgttg ctcaaaaacc atccctttca acgcatggag
    20401 agaatcgctc aggttggcga taccgatccc ctgtacgccg gagaagttat aacgcgcgcc
    20461 gccatcggta atatcgcgac ctttttccag acaatcgctg ataaaagacg agagcagcgg
    20521 tacaggtgcc cagtcgcgat ggccgatatc acaaatattg ctgccctcaa ccatcagggt
    20581 gatgtagtgg ctgatcttgg cgcggatctg ctccagtaaa ccttcataag tcagcgcagc
    20641 attgccttca ttttcatgca ggcagatttc catcactttc agcagattaa acatcgcgat
    20701 gtcatgcaag ccgtaggttc tgccgggaat agataattcc acacagccca ctacggaata
    20761 gtcgcgcgca tcttccagcg aaacgccacg gttgaggaac gctggtacca ccacttcatc
    20821 gttaaagatt tgcggaatac cggtgccgag gcgaatggtt tcggcggttt tcatcaggaa
    20881 tggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc ggttgcggta attgcacgct
    20941 ttgataggcg tcaaggcaaa ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt
    21001 taacccgccg agcagtgcgg tatagccggt cgggaaacct gcgaaataac gcgcgctgct
    21061 ggtggagcgc aacagcacga tgtcgttgca tttcacccat aaagattcga gcagttcttt
    21121 caggaacgcc ggatcttcgc cctgggttaa agatgcctga tagaacggca acatatactg
    21181 gtcgaagcgc cctaaagaca gcgaactggc gttggattcg tattgcagaa tgatgttcat
    21241 gtaccagaat aactggcacg cctgccagaa cgtctgcggc ttatgttcgg cgttatggcg
    21301 agagatctcc gcaatagtca gcagttcttc gcgacgctgg gcatctgtgc agtttgccgc
    21361 catcgtttcc gccagtacag cgtaacgcaa aatgtgtttc tgcgaggctt ccagcagtaa
    21421 cagcgctgcc tgataaaagt gattctccgg ctgttgctga caatgttgct gcatctgtgc
    21481 caccaactcc cccagcccgt gattcaacag gcgtgggtaa tcaataataa tgtgcccctg
    21541 gcctttatcc gtctggttaa tgctgaaaat ctgcgtgctg gtcgcggctt ttacttcatc
    21601 cgtcatctgc ccgttgatga aatctttcat cgaacgtttt tcccagtacg ggaacaactc
    21661 ttcacgatag atacgtttat cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg
    21721 gaattgatcc agctctttca gcagccagta agggtccatt tccggcgaca taatcccggc
    21781 gcgcggtttt acggtgcggt taccggcaat cagttcttca tcacgaatcg aaatttcaac
    21841 atgttcaagg atatacgctg ttgctttcgc ccggcgcaat atcaccggtt cgccttcggt
    21901 ttgccgatgg ctggcggtat aaagcagcgc ccgctccagc gagatttcac gggtattggc
    21961 aaacagtgca gttttgaggc gagagatacg attcgtcatg aggaactcct gtgggaaagc
    22021 gaacccgccc ttgcgagcgg gagaagaact taagcggttt gcgccaggtg agcttcaatt
    22081 ttgctcataa tggcgtcagc acgttttact gcatcgctga tgttgacgcg cacgatggtc
    22141 ttgccagcaa aacgctcttc aaacttgatg ccgatatctt tggtcagaat caccatatca
    22201 gcgctcgcca catcttccgc agtcagttca ttttccagac caattgaccc ctgggtttct
    22261 actttcactt cccagccttt cgctttcgcg gcactttccc gcgcctctgc tgccatatag
    22321 gtatgggcaa cacctgaagg acatgcggtt actgcaataa ttttcgtcat ggctcgttcc
    22381 tcaattaatt aatttcaaaa tccaaatcca ggtcgtcttc tttttcatca gtcgaagacc
    22441 catttttacg cgccagactt ttcagcacgt tcacacaaac agccgtcacc actgctccga
    22501 cagccactgc tgcgatataa cccagcttgc cttcaaccac cggcagcaca atcagaccac
    22561 cccagcctgc atagcactgc gcaccgacca gcgccgcagt tactgcacca caaactgaac
    22621 cgaccatgat ggaaggaatg acacgcagcg gatcggcagc ggcgaaagga atcgcccctt
    22681 cagtaacgcc aacacagccc atcaccagtg ccgctttgcc ggtttcgcgc tcttctgcgg
    22741 agaaattttt acgaccaatc agcgtcgcca gccccatccc cagcggcggg atacaaatac
    22801 ccacggcagc gatgaccacc acggtataaa caccctgagc aacgcaaatc agcatgaagg
    22861 cataggctac tttgttaacc ggaccgccca tatcgaacgc cagcatcaga cccatgatca
    22921 ccgccagcat aacaatgctg ccctgctgca tcccctgaag ccactgagtc aggctgttgg
    22981 tcaacgcccc tacaggctcg cccaagcccc acatcatgat gcctgcggta atcagtgtgc
    23041 cgacgatagg aataatgaag ataggcatca ccgagcgcag aactttatgc accggaattt
    23101 tcttcaggta atgcaccacg atgccgccga taatcccggc gatcagtgca ccaaagaagc
    23161 ccgcaccaaa gctgttacca acccaggcac cgatagcgca cggagccagc gcagaacgct
    23221 ctgcaatgga gtaaccaatg taagcggcga ggaaaggcac catcagcgtc aaacctgcaa
    23281 cgccgatatc aaacagtttt ttcaaattcg gatcggctac ggcatccggc actgcgcctt
    23341 tgccatacaa catgacggaa accgccagca aaataccgcc cgataccacg aagggaatca
    23401 tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt caggatctgc accaactcat
    23461 tcataagctg ctcccgggct tgcgaaagcc catgtccata tttttgatgg aggaaacaat
    23521 aggcaatcgc aggcggagca aacagtggat aaaaaggcca tttactggac ttttgtaata
    23581 tcagtacaaa aatgcgatcc gcctcataac ttgcgataaa ccggaagcat gccgcaaaac
    23641 ggcgtcttgt gagacgcgtc gcaaattgac ggttacatta catttgtcca gcttttggca
    23701 gatttgtcac atggcaggcc gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc
    23761 gggagagagg caatggccct gattgtggaa tttatttgtg agctacctaa cggcgtacat
    23821 gcgcgtccgg caagccacgt tgaaacgctg tgtaatactt tttcatcaca aattgagtgg
    23881 cataacctgc gcactgaccg caagggcaac gccaaaagcg cccttgcgct gattggcacc
    23941 gatacgctgg cgggcgataa ctgccagtta ctgatttccg gggccgacga acaggaagcg
    24001 caccagcgtt taagccaatg gctgcgcgat gaattccccc actgcgatgc gccgctggcg
    24061 gaagttaaat ctgacgaact ggaaccactg ccggtttcac tgaccaatct gaatccgcaa
    24121 attatccgcg cccgcaccgt gtgcagcggt agtgcaggcg gcattctgac gccgatctct
    24181 tctttagatc tcaatgcgct gagtaatctt cccgcagcca aagacgttga cgccgagcaa
    24241 tccgcactgg aaaatggcct gacgctggta ctgaaaaaca ttgagtttcg tctgctggat
    24301 agcgacggtg ctaccagcgc gatactggaa gctcaccgat ccctggctgg cgatacatct
    24361 ttacgtcagc acttactgac aggcgtcagc gagggattaa gctgtgccga agcgattgtc
    24421 gccagtgcga atcacttttg cgaagagttc gcccgttcca gcagcagcta cctgcaagaa
    24481 cgtgccctgg acgtacgcga cgtctgcttc cagttactcc agcaaatcta cggtgagcaa
    24541 cgcttcccgg caccgggcaa actgacgcag cccgccattt gtatggctga tgaactgact
    24601 cccagccagt tcctcgaact ggataaaaat cacctcaaag gtctgttact gaaaagtggc
    24661 ggcaccacct cacataccgt aattcttgcc cgttcgttca acattccgac actggttggt
    24721 gtagatattg atgcccttac tccgtggcag catcaaacga tttatatcga cggcaacgcc
    24781 ggggcgattg tggttgagcc aggggaagcc gtggcccgtt attatcagca agaagcccgc
    24841 gtacaggacg cccttcgtga gcaacagcgt gtctggctga cccaacaagc ccgtaccgct
    24901 gacggtatcc gcattgaaat tgccgctaac atcgctcact ccgtggaagc gcaggccgca
    24961 ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg aaatgctcta tatggatcgc
    25021 accagtgcac cgggcgaaag cgagctgtac aacatttttt gtcaggcgct ggagtctgcc
    25081 aacggacgca gcattattgt gcgcactatg gacattggcg gcgacaaacc cgttgattat
    25141 ctgaacattc cggcagaagc taacccgttc ctcggttatc gcgctgtgcg tatttatgaa
    25201 gaatacgcct cgctgttcac cacacaacta cggtcgatcc tgcgcgcctc cgctcacggc
    25261 agcctgaaaa tcatgatccc gatgatctcc tcaatggaag agatcttatg ggtgaaagaa
    25321 aaactggcgg aagccaaaca gcaactgcgt aacgaacaca ttccgtttga tgagaagatc
    25381 cagcttggca ttatgctgga agtaccgtcg gtgatgttca tcatcgatca atgctgcgaa
    25441 gagattgatt tctttagtat tggtagtaat gacctgacgc aatatctgct ggcagtggat
    25501 cgcgataacg ctaaggttac tcgtcactac aacagcctga atccggcatt cttgcgggcg
    25561 ctcgattacg ccgtgcaggc ggtgcatcgc cagggcaaat ggattggtct gtgcggtgag
    25621 ctgggagcga aaggttccgt gctgccgttg ctggtcggct tagggctgga tgaactcagc
    25681 atgagcgcac catcaattcc ggcggcgaaa gcgcggatga cgcaacttga tagccgtgag
    25741 tgccgcaagt tgctcaacca ggcaatggcc tgccgtactt cgctggaagt ggaacacctg
    25801 ctggcgcaat tccgcatgac ccaacaggac gcaccgctgg tcaccgccga gtgcatcacg
    25861 ctggaaagtg actggcgcag caaagaagaa gtgctcaaag gcatgaccga taacctgctg
    25921 ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg acttatgggc gcgcgaggcc
    25981 gttttctcta ccggtctggg ctttagtttt gccattccac acagcaaatc agaacacatt
    26041 gagcaatcca ccatcagcgt ggcgcgtctg caagcgccgg tgcgctgggg cgatgatgaa
    26101 gcgcaattca tcattatgtt aaccctgaac aaacacgctg cgggcgatca gcacatgcgc
    26161 attttctctc gcctcgctcg ccgcatcatg cacgaagaat tccgtaacgc gctggttaac
    26221 gccgcctctg ccgacgctat cgccagcctg ctgcaacatg aactggaact gtaaaaggaa
    26281 acatcatgga actgtatctg gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca
    26341 tattccccat tgccggggtg acaactaacc cgagcattat cgctgccagc aaggagtcca
    26401 tctgggaagt tctgccgcgt ctgcaaaaag cgattggtga tgagggcatt ctgtttgctc
    26461 agactatgag tcgtgacgcg cagggtatgg tggaagaagc gaagcgcctg cgcgacgcca
    26521 ttccgggcat tgtggtgaaa atcccggtga cttccgaagg tctggccgcc attaagatgc
    26581 tgaaaaaaga gggtattact acgcttggca ccgcagtgta cagtgctgca caagggttac
    26641 tggccgcgct ggcaggggca aaatacgttg ctccgtatgt taaccgcgta gatgcccagg
    26701 gcggggacgg cattcgtacg gttcaggagc tgcaagcgct gttagaaatg cacgcaccag
    26761 aaagcatggt gctggcggcc agctttaaaa cgccgcgtca ggcgctggac tgtttactgg
    26821 caggatgtga atccatcacc ctgcccttag atgtagcgca acaaatgctc aacacccctg
    26881 cggtagagtc agctatagag aagttcgaac acgactggaa tgccgcattt ggcactactc
    26941 atctctaaag gagcaattat ggaccgcatt attcaatcac cgggtaaata catccagggc
    27001 gctgatgtga ttaatcgtct gggcgaatac ctgaagccgc tggcagaacg ctggttagtg
    27061 gtgggtgaca aatttgtttt aggttttgct caatccactg tcgagaaaag ctttaaagat
    27121 gctggactgg tagtagaaat tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgat
    27181 cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga
    27241 aaaaccctcg atactgccaa agcactggca catttcatgg gtgttccggt agcgatcgca
    27301 ccgactatcg cctctaccga tgcaccgtgc agcgcattgt ctgttatcta caccgatgag
    27361 ggtgagtttg accgctatct actgttgcca aataacccga atatggtcat tgtcgacacc
    27421 aaaatcgtcg ctggcgctcc tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca
    27481 acctggtttg aagcacgcgc ctgttcccgt agcggcgcta ccactatggc gggcggcaag
    27541 tgcacccagg cggcgctggc actggcagaa ctgtgctaca acactctgct ggaagaaggc
    27601 gaaaaagcga tgcttgccgc cgaacagcat gtagtaaccc ctgcgctgga acgcgtgatt
    27661 gaagcgaaca cctatttaag cggtgttggt tttgaaagcg gcggactggc ggcggctcat
    27721 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    27781 gtggcattcg gtacgctgac gcagctggta ctggaaaacg cgccggttga ggaaatcgaa
    27841 accgtagctg catttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    27901 aaagaagatg tcccggcgaa aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa
    27961 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccac tctgctggta
    28021 gccgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    28081 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    28141 tctctgcaat atattgaatt tgcatgcttt ttaggccaga taaggcgttc acgccgcatc
    28201 cggcactctg tgccagtaat ctgaaactcc tacgttgttc aggagttttt tatccgcttc
    28261 tggacatttt ctctacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    28321 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    28381 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    28441 aaccttgcca tcgcagacgg tgccagacag caaatcggca acacgctatt ttacgatccg
    28501 gcgtatgtgc ggcttaccta tcctggcggt gatgtcccgc aagaacgcgg tgtatgttcc
    28561 gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    28621 gatatggcga agaattttgc cgagtacccg caaaaatgga agttaaagcg cccggacagc
    28681 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    28741 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    28801 gataacgggc tggcgcatat tggggtggtg tcagatggct tcgcccgcga cggtacgcca
    28861 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    28921 atggtggggc attatcgtta ttttgttaaa taagcgatcg ccccacatga agaaaaaatt
    28981 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    29041 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    29101 gaaaagagta cgccgcaaac gggaacggcc agcgtgctta agctggcaat actggcaggc
    29161 aagtttttca atacaaacaa ccataagctc cacgccagcg ccgtcgccag aatcgcactg
    29221 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    29281 gcgaccacac tcatcaccag cgccgcatag agcatctgcc aggatgttaa cgataataaa
    29341 tccacgcgcg gatgacgggt gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    29401 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    29461 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    29521 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    29581 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    29641 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gtgcattccg
    29701 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    29761 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    29821 cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt tcctgcggca
    29881 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    29941 agcgactgcc tgaaagacag tcactgaaga aggtcagatt acagcaggtc gaaacggtcg
    30001 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    30061 ctgctggcgt aaacttccgc taccgcacgc aggacagagt tagaaccaaa taccagatcc
    30121 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    30181 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    30241 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgctatc gaagttggca
    30301 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    30361 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    30421 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    30481 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    30541 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    30601 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    30661 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    30721 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    30781 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    30841 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    30901 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    30961 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    31021 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    31081 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    31141 tgtacccact catatttgaa caggttctcg aagaaatagt tgctccactg ggtcggcgtc
    31201 tgggtccaga caacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    31261 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctaca
    31321 tttgatgtcg gaccggcacc gtgagtttta cccagcgtat gaccacccgc aatcagcgcc
    31381 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cacggatagc tgctgccgca
    31441 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag acccatctcg
    31501 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    31561 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    31621 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    31681 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    31741 tcaaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    31801 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaggc catacgaata
    31861 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    31921 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    31981 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    32041 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    32101 tgatggaacg ggcatttgcc agtggctgtc gtgttatgga tatcgtctga cgtgctcatc
    32161 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    32221 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    32281 gtcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    32341 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    32401 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    32461 gtgtgatttt cacaaaaacc acactattta taaaccaggt cgaacaccca gcgtatggca
    32521 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    32581 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    32641 ggcatcatca tccaggccgt cgaacatttg cgccatccac gccggaatac gcacgttggt
    32701 catatcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaattc ccggaataat
    32761 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    32821 gaagaagaac tgagtaatcg cgcggttggc tccggcatcg actttgcgtt tcagattaag
    32881 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggagat
    32941 atcgaaatct gccacttctt ttaatagcgt caccaggtca gaagcataca tttctggctt
    33001 accacttccc ggcggcagat cgccacgcag cgccacgata tgacgaatac cgttattcca
    33061 gtagtcgcgt gcaatggtgc gcaactcgtc gggcgtcgca tcaatgcaag taagatgcgg
    33121 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    33181 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    33241 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    33301 cgaaacgtta atctgcccct ggacttctgc caggcgctga ttcagggcat cccgctggct
    33361 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    33421 cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    33481 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    33541 aagaaaaaga aaatccggta tgcaaagcaa accggactgc aaatataaac taaccatgta
    33601 cttattgctt cttgccggat tcgtcgtaaa cgcttaatcc ggcctaatcc tatgataatt
    33661 caatatattt cattagaaac cataaacccg aaaacatgag taccgggcat tattaaattt
    33721 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    33781 gtcgcgcccc gcgccaggcc cacggatcac cagcgggttg tcgcgatacc agcggctttc
    33841 gatggcaaag acgttatcgc acggcagcag tgatgccagc ggatggtctt cacgcaccgc
    33901 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    33961 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    34021 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    34081 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaattacca gcttgcgcat
    34141 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    34201 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    34261 cgtgccggag aagatcccgc tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg
    34321 cacggtgtgg ttgatcggca agcccgcacc gacggtggca ttgtacagcc agtgacgccc
    34381 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgctgg ctcccgccag
    34441 tttgttggcg ctgataacgt ggaaaccgtg gctggcgaaa tcaagatact gatcagcaag
    34501 ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg cgcgcatcca
    34561 caggaacaac gactcttcat cctgctcaac cgcttcatcg ttgaagaagg ctaacgcgcg
    34621 gctggcgtcc agcccgtcat agctcaacag gctgcggcgg ctgtccacca cacctgccag
    34681 cacaaactca aagccggtac gtgccgaaag cgtgctctgc tcacgggcga acagttccag
    34741 ccaacgggaa ccgatattgc ccttaccgaa caataccagg ccgatgcgtt tttctgcgcg
    34801 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    34861 caccaggctg atgccgtcat cggactgcca ggtaaattcg accggctggc ctttcagttg
    34921 ctgccagaag cggtggcaat gcagcgggtt acgggtgacg cctgcaccga ccatcgccac
    34981 cagcgccagc ccctgacgca ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt
    35041 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
    35101 tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg atttggtcga tctctttatg
    35161 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    35221 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    35281 ttgatccggc gtgtagctac agcgcagttg caggtcgatt tcgctgccag aaaccggctg
    35341 taaagtacgg gcgtgaagaa cgggagccgc caggcgcgcc agttcgctgg cctcatccag
    35401 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    35461 ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc agcgcaccga tttgtgtcgc
    35521 ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt tgcggctgat
    35581 aaatccggtc accaccagac gtttgcccgg atgttgcacc agcagctgtt gcagcaacgg
    35641 gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt tcagcgcgta aaaactcgcg
    35701 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgtgc
    35761 cgaccatact tccccgtggc ccaccacttc cgcatacact gcgtcgttaa taccgctgtc
    35821 gagcagcgcc gccaggcgct caaggtcgct gacaaaagcg ctaatgaggc tatcagcttc
    35881 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    35941 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    36001 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact ccgccataat
    36061 gcccgcaaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    36121 ttgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    36181 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    36241 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    36301 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    36361 aacaacgaca gcccgcccag gaaacgacgc agcgtctgct catcgccatc cagttcaaaa
    36421 ctcaacattg cgccaaagcc tttttgctgg cgcgcggcaa tttcatgccc ctgattttcc
    36481 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    36541 gcctgcgcgt tgcgctgggc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    36601 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgtcca ccaggcaagt
    36661 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    36721 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    36781 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    36841 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    36901 accagtttag gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    36961 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    37021 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccaag
    37081 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    37141 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    37201 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    37261 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    37321 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    37381 ttaatggacg tcaatacatc tggacatcta aacttcttta cgtatagatt gagcaaatcc
    37441 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    37501 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtgtggt
    37561 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    37621 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    37681 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    37741 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    37801 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    37861 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagca
    37921 aaaaagagcg gcgcggagtg gaatcgcctg atgcgctacg cttatcaggc ctacgtcata
    37981 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    38041 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    38101 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    38161 cacaaaccgg cgacatcatc tttcagatct ctcgctcatc gcaaagtaaa gcgatccaac
    38221 tggcgaccca ttccgattat agccacaccg gtatgctggt gatacgcaac aaaaagccct
    38281 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg atcgcccatg
    38341 gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    38401 aaaaactggc gcaaacggta aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    38461 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    38521 gaatgcgcgt gggcgagcag cagaagttga aagagttcga tctcagcaac ccgctggtcc
    38581 aggctaagct caaagaacgc tacggcaaaa atattccgct tgaggaaacg gtagtttcac
    38641 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    38701 cgtggtaatg gatgatgaaa aaaataattc ccgccgtagt ctgtttgttg ctttgtagtt
    38761 gcacgacctt tccgcaaact gtcgctccgg taaacggtga tgcctccaat tagttgaatt
    38821 aaggatataa tactcgcttt taaggttgtt tcatggtcac agttaacctt cattgccccc
    38881 gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc aaaaagacat gatcgattcc
    38941 gcggccgtga ctgtcaccgg gtatttcagc tcagttactg ttacgagccc cgtaaaccgg
    39001 gagttaaaga ccaaatcgct gaaatgactt tcaacggtgc cggggttcgc gatactgccc
    39061 gtacgctgaa aatcggcatt aatacagtca tccgaacttt aaaaaactct cgccacgaag
    39121 aataacgtca tccccggtcg ctcatgctga tgtggcactc atctgcgaac ttagtcagca
    39181 atggagcttt gtcggcagta aagcgcgaca gcactggctc tggtacgtgt acaacacgaa
    39241 aactggtggt gttctggcct acacttttgg cccccggact gacgaaacct gtcgtgaatt
    39301 gctggcgctg cttacccttt taccatcggc atgttgac
//
LOCUS       DAJVTY010000016        37322 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVTY010000016 DAJVTY010000000
VERSION     DAJVTY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37322
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.016"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(2..811)
                     /locus_tag="OQQ66_000782"
     CDS             complement(2..811)
                     /locus_tag="OQQ66_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCS1903802.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(825..1256)
                     /locus_tag="OQQ66_000783"
     CDS             complement(825..1256)
                     /locus_tag="OQQ66_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903803.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1256..1459)
                     /locus_tag="OQQ66_000784"
     CDS             complement(1256..1459)
                     /locus_tag="OQQ66_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS1903804.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1572..2420)
                     /locus_tag="OQQ66_000785"
     CDS             complement(1572..2420)
                     /locus_tag="OQQ66_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903805.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILDIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2516..3775)
                     /locus_tag="OQQ66_000786"
     CDS             complement(2516..3775)
                     /locus_tag="OQQ66_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1903806.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3966..4829)
                     /locus_tag="OQQ66_000787"
     CDS             complement(3966..4829)
                     /locus_tag="OQQ66_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1903807.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4956..5672
                     /gene="rph"
                     /locus_tag="OQQ66_000788"
     CDS             4956..5672
                     /gene="rph"
                     /locus_tag="OQQ66_000788"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1903808.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5738..6379
                     /gene="pyrE"
                     /locus_tag="OQQ66_000789"
     CDS             5738..6379
                     /gene="pyrE"
                     /locus_tag="OQQ66_000789"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1903809.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6416..7012)
                     /gene="slmA"
                     /locus_tag="OQQ66_000790"
     CDS             complement(6416..7012)
                     /gene="slmA"
                     /locus_tag="OQQ66_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1903810.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7119..7577)
                     /gene="dut"
                     /locus_tag="OQQ66_000791"
     CDS             complement(7119..7577)
                     /gene="dut"
                     /locus_tag="OQQ66_000791"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1903811.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7555..8775)
                     /gene="coaBC"
                     /locus_tag="OQQ66_000792"
     CDS             complement(7555..8775)
                     /gene="coaBC"
                     /locus_tag="OQQ66_000792"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1903812.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8947..9615
                     /gene="radC"
                     /locus_tag="OQQ66_000793"
     CDS             8947..9615
                     /gene="radC"
                     /locus_tag="OQQ66_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1903813.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9832..10068
                     /gene="rpmB"
                     /locus_tag="OQQ66_000794"
     CDS             9832..10068
                     /gene="rpmB"
                     /locus_tag="OQQ66_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1903814.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10089..10256
                     /gene="rpmG"
                     /locus_tag="OQQ66_000795"
     CDS             10089..10256
                     /gene="rpmG"
                     /locus_tag="OQQ66_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1903815.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10354..11163
                     /gene="mutM"
                     /locus_tag="OQQ66_000796"
     CDS             10354..11163
                     /gene="mutM"
                     /locus_tag="OQQ66_000796"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1903816.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11202..11681)
                     /gene="coaD"
                     /locus_tag="OQQ66_000797"
     CDS             complement(11202..11681)
                     /gene="coaD"
                     /locus_tag="OQQ66_000797"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1903817.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11689..12966)
                     /gene="waaA"
                     /locus_tag="OQQ66_000798"
     CDS             complement(11689..12966)
                     /gene="waaA"
                     /locus_tag="OQQ66_000798"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1903818.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13379..14437
                     /gene="rfaQ"
                     /locus_tag="OQQ66_000799"
     CDS             13379..14437
                     /gene="rfaQ"
                     /locus_tag="OQQ66_000799"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1903819.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14434..15558
                     /locus_tag="OQQ66_000800"
     CDS             14434..15558
                     /locus_tag="OQQ66_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1903820.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15551..16348
                     /gene="rfaP"
                     /locus_tag="OQQ66_000801"
     CDS             15551..16348
                     /gene="rfaP"
                     /locus_tag="OQQ66_000801"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1903821.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16364..17380
                     /gene="waaO"
                     /locus_tag="OQQ66_000802"
                     /gene_synonym="rfaI"
     CDS             16364..17380
                     /gene="waaO"
                     /locus_tag="OQQ66_000802"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1903822.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17397..18392
                     /locus_tag="OQQ66_000803"
     CDS             17397..18392
                     /locus_tag="OQQ66_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS1903823.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18402..19094
                     /gene="rfaY"
                     /locus_tag="OQQ66_000804"
     CDS             18402..19094
                     /gene="rfaY"
                     /locus_tag="OQQ66_000804"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1903824.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19120..20145
                     /locus_tag="OQQ66_000805"
     CDS             19120..20145
                     /locus_tag="OQQ66_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS1903825.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20230..21213
                     /locus_tag="OQQ66_000806"
     CDS             20230..21213
                     /locus_tag="OQQ66_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1903826.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRYCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21259..22512
                     /locus_tag="OQQ66_000807"
     CDS             21259..22512
                     /locus_tag="OQQ66_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1903827.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22582..23562)
                     /gene="rfaC"
                     /locus_tag="OQQ66_000808"
     CDS             complement(22582..23562)
                     /gene="rfaC"
                     /locus_tag="OQQ66_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1903828.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(23566..24612)
                     /gene="rfaF"
                     /locus_tag="OQQ66_000809"
     CDS             complement(23566..24612)
                     /gene="rfaF"
                     /locus_tag="OQQ66_000809"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1903829.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24622..25554)
                     /gene="rfaD"
                     /locus_tag="OQQ66_000810"
     CDS             complement(24622..25554)
                     /gene="rfaD"
                     /locus_tag="OQQ66_000810"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1903830.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25768..26964
                     /gene="kbl"
                     /locus_tag="OQQ66_000811"
     CDS             25768..26964
                     /gene="kbl"
                     /locus_tag="OQQ66_000811"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1903831.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26974..27999
                     /gene="tdh"
                     /locus_tag="OQQ66_000812"
     CDS             26974..27999
                     /gene="tdh"
                     /locus_tag="OQQ66_000812"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1903832.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28238..29272
                     /gene="waaH"
                     /locus_tag="OQQ66_000813"
                     /pseudo
     CDS             28238..29272
                     /gene="waaH"
                     /locus_tag="OQQ66_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29259..30218)
                     /locus_tag="OQQ66_000814"
     CDS             complement(29259..30218)
                     /locus_tag="OQQ66_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1903833.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30222..31505)
                     /gene="envC"
                     /locus_tag="OQQ66_000815"
     CDS             complement(30222..31505)
                     /gene="envC"
                     /locus_tag="OQQ66_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1903834.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31515..33059)
                     /gene="gpmM"
                     /locus_tag="OQQ66_000816"
                     /gene_synonym="pgmI"
     CDS             complement(31515..33059)
                     /gene="gpmM"
                     /locus_tag="OQQ66_000816"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1903835.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRNPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33304..33735
                     /locus_tag="OQQ66_000817"
     CDS             33304..33735
                     /locus_tag="OQQ66_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1903836.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33877..34128
                     /gene="grxC"
                     /locus_tag="OQQ66_000818"
     CDS             33877..34128
                     /gene="grxC"
                     /locus_tag="OQQ66_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1903837.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34191..34658
                     /gene="secB"
                     /locus_tag="OQQ66_000819"
     CDS             34191..34658
                     /gene="secB"
                     /locus_tag="OQQ66_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1903838.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34658..35677
                     /gene="gpsA"
                     /locus_tag="OQQ66_000820"
     CDS             34658..35677
                     /gene="gpsA"
                     /locus_tag="OQQ66_000820"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1903839.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35757..36578
                     /gene="cysE"
                     /locus_tag="OQQ66_000821"
     CDS             35757..36578
                     /gene="cysE"
                     /locus_tag="OQQ66_000821"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1903840.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36631..37104)
                     /gene="trmL"
                     /locus_tag="OQQ66_000822"
     CDS             complement(36631..37104)
                     /gene="trmL"
                     /locus_tag="OQQ66_000822"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1903841.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9248 a         8882 c         9298 g         9894 t
ORIGIN      
        1 gttacgcatg gtttacctcc agacgtttag cgagccagcg ttgcgacagg cgggtgagtt
       61 ctcctttgcg ctgctcatat tccatgccca tatcaattaa cgtgatattg gtgctttcga
      121 gataggcgag gtgttcaagc tgtgctgcgt tcatgctgtc ccgtggttcg ccagtaatgc
      181 catttgcctg cgcccactgc ttcgccgtca tgccacccag cacgatacgg gcgatcatgt
      241 tgctctcgtt gctgtagtgg cgctgctgcg tcgttttgcc catctcggca cgggccatat
      301 tcagcgcatc gcacattggc ttaaagttgt ttgcggcact gagacgggct tttagctggc
      361 gacgatagcg ggcggcgatt tcaggcacgc tacgctgtaa ttcttcctcg cactggatga
      421 aataacggcg gatagcgcgg ccctgctcgt tgcgttcaac cattgccact tctttagcca
      481 tatccagcga tagcaggtaa tcatgctcta tttgctggcg agtttttgcg ctcgcccgtt
      541 ttggcgtgct caaattttca acacggataa aatcggttcc ggcaataaaa ccgtactggt
      601 caattcggcc tttaatccag ttagtaaagt cgcggcccac ccccagcgct ttatgcaacg
      661 ccttagcgct cacaatattg gcttcacgcc cgccaatgcg tccggcaata acgggaacaa
      721 tagcggcaaa gtcgttgccg ttaattactc ccgtgttaac gttgggttgc ggggcggccc
      781 ccgaactgaa tcggcttttt tcgatagtca tattttcggc tccgttatgc ggcggtgaga
      841 ttgtccgggt atagattgag aatgtcggca atttcaggct gagaaagtcc ctgatagccg
      901 ccagcgtcgg cgttatggtt cacaagccga ataactttta gaacgtcacc gcgcccggta
      961 aagcgatagc gtaaatgtga ccctatcccg tcggggttct tttcgtctat acgctcaagc
     1021 tgaatatcaa gcctgcgctc taactcgctg gcgtagttcc ttccagacga aaggcggcaa
     1081 taacgcagaa tgtcattttc tgtccatccc tctataccag tacgcagcat atagactctg
     1141 gcacgatgct ttttcggtgt gcgctttggt gcttgaaggg attttgtaag gggcgtaaca
     1201 tcagatacgc gaatatctga attagccgcc tgtaatgggc ggtttttctt ttccatcagg
     1261 ccaccttccc gcggcgttct gccagccagt tgtgaatctc aaccgcatca aacgcagtta
     1321 ttttttctcc cagctttacc ggacgtggta gtgaaccgtt tcttacttta cggtcgatgg
     1381 tggatacact aacgcccagc aattcagcca ggcggaagcg tctgatatag ccttttgtag
     1441 ggatagtgtc gatgttcatg attcccgtaa cctcttagcg atagttgttg cttcgttacg
     1501 ggtatttaat ggcgtgcacg ctcaaaaagg aatgatcgta ttttttacta tcgtgatagt
     1561 aaaatttact atcacctttt tttccctgcc gtggtgcgtt cttccggtgt tctgtctgcg
     1621 tcggctatga gtttcttcag gtaatctatg gatggctcat catagccgcc ccccaaaagc
     1681 gcccagtggt ttattgtggc gttcgcccat gcttcgttgg tttccttccc gttcttccct
     1741 ttacactctt ctgggtagtg ccgcttcaca taaatcaacg ccaagaggca aatttcgcgc
     1801 ttttgcctgt ggcgctctct gtttccgtgc tcaacgtttt taacatcctc ataaagagct
     1861 tgtaagttta tgcgagtttc ggggctgtat atgggctggt ttttgtccat gcattcgccg
     1921 atatattcaa cccacggcct tgttatgtac aaatcctcta cggatatttc gtggaaggca
     1981 tgttttgtat aatcgagaaa aaaataataa ttcgcactta cgtctagttc tctttttctg
     2041 tgtgcggggc tgaatatgcg tgcgggagtg tcaatagaat caagccctgc gatgtcgagt
     2101 atataggagg cgttaacatg ccatagccca agcacatggc aatctatatg atttacaacg
     2161 cctgccttaa agcttttttc gtcctctact tcagtgatta tgtcggcaag taataagcag
     2221 tcttctttga cgtctgggaa taaagagcaa ttgcggtaaa gactcgcgat gctatgtaat
     2281 gtgccgtcgt agccgcaaag gtacgcgctc aaatatatag attctgccgc cgccataatc
     2341 aaaagatcat caatatctat ttctaataat tcggctgctc tgttaagcct gaaaaaaggg
     2401 gcgggtggta attttttcat tgtgtcacct tttacgctgc gccttatttt gtgattgagc
     2461 caggcggtaa ggcttcccgc ttttcgcccc gtcgagctag gctcaatcta ttcagttagt
     2521 tcgcccttct gagaggcgtt acgttgtagt cttttccgct ttccagtgcc accagcagat
     2581 cagcccactg tgccagtgct gctttacgct catcaaaata ctgatggcgg ttataaatcc
     2641 cctctacgcc cttaatgcga tgattgaggc atcgttccgc cacaacagga tcaacaccca
     2701 acgctgctaa atgagtacgt gcggtgcgtc gaaagtcgtg aatcgtgaaa ttaggcacat
     2761 ccggcatttc ggcgcgaacc ttagccagtg ctacgggtaa agtgctttcc tgaatatgtg
     2821 ggatcattct gttttgcatc ttcctggccg gaagcaccca tgcgctatta catgaaaagg
     2881 tgtgtagctc tctcaaccat tcaacggctg gggaaggtag aggtatatca atagggtctc
     2941 cgtttttgct gcgttcttcc ggcaaatgcc ataccgcacc atctaaatca aactcttccc
     3001 atcgtgcggc gcataattcc attttgcgga cgcataacgc taacagtaat ttgaacgtga
     3061 tttcgttctg gcgactgaat cccttagccg tgcgcatggc tttgaatagc tggattaact
     3121 catcgcgggt taaccagcgg tcacgggcag cttctttccc tccggcgtct gccacctcaa
     3181 aggctgaaca ggggttaatc tctagcgcgt gccgtttgat tccgtagtcg aatatgcggc
     3241 gcgtccatct cagcacgtcc gttgctatgg tcggcgctcc acggtcaaca atacctttca
     3301 gcatgtcatc gatatggcgc ggtttcacgt cctctacctt catgctgcca atgcaggggt
     3361 ttatatcttt gtcgatacgg cggcggagta tatcggggtg cttccagcgc gggaggatct
     3421 gacgctcaaa gtattcagcg gcaagctctg aaacgcgcat agcgttcttc tctgcttcca
     3481 tcttcgccag tgcttcggtt ttgcgcttct gcttctctcc tgcaacgtca tagcccagcg
     3541 caacgcgagc cgatagctct ttggctgtct ctctggcctt tgatagtgat agctcgctat
     3601 acgaaccaat tagcatggcg cgggacttcc ctgcgagttt atagcggaat ctccatgtgg
     3661 gggttttgtc ggcttcacgg taacgtaggt ataaaccgtt accgtctgcc cgcccctcga
     3721 atcgctctcc gctcttaatc catgcgcgga tctgcatgtc tgttagtttt ggcattttgc
     3781 gaatatccaa actttgaata gggggtgtac ccaaagcaat gtacccaaac ctcattgggt
     3841 acacctttgt gtacaccata atcatgcgct ttgttgcgtc tttatgatag cccatgataa
     3901 caaaaaagcc cgctgggttg cgggcttggt gtgatttatg cgttgtccta aatgttgctg
     3961 atagcttact cgatattctg aatctgctcg cgcatctgct caatcaacac tttcagctca
     4021 atcgcggagt ttgtcacttc ggcattgata gacttcgacg caagagtgtt cgactcgcgg
     4081 ttgaattcct gcatcataaa gtccagacga cgaccaacgg cttctttttt cttcagaatg
     4141 ttgtaggtct ctttgacatg cgcttccagg cggtccagtt cttcggcaac gtcaattcgt
     4201 tgtgccagca gaaccagctc ctgctccaga cggttgtttt ccaactgaac ctgagcatct
     4261 tccagcttcg cgaccagacg ctcacgctgc cattgcagga tttccggcat atgggcgcgg
     4321 actttgacca cttcggcggt gacgccttcc agacgctgct cgatcaatgc tttcagtgcc
     4381 tgaccttcgg tttcgcgcgc gacaataaag tcgtccagcg taccatccag cgccgcgaga
     4441 atttcagcgg caatggcgtc aagatcctgc tcctgggctg ccatcacgcc cggccagcgt
     4501 agaatatcaa ccgggttgat ttccccttcg tcactctgca tttttaccca gttcgcggca
     4561 gttaccagct gtttagccag tttttcgttg aggatcagct caccttgcgc gctaatatct
     4621 ggctcatagc gcagggtaca ttccacttta ccgcgcgtca ggcgagaacg aatacgctcg
     4681 cgaacgacag gttcaaggct acggaactgc tccggcagac gaaagtaagt ttccagataa
     4741 cgctggttta ccgagcgcat ttcccaggtt gcgctccccc attcaccctt gatttcacgc
     4801 cgggcgtagg cggtcatact gcggatcata gacgttcctg tttataaaag gagaggtgga
     4861 aggattatag ccatcgatgc cttgtcagga taggaataac cgccggaagt ccgtataatg
     4921 cgcagccaca tttgtttcaa gccggagatt tcaatatgcg tccagcaggc cgtagcaata
     4981 atcaggtgcg tcccgttacc ctgactcgta actatacaaa acatgcagaa ggctcggtgc
     5041 tggtcgaatt tggcgatacc aaagtgttgt gtaccgcctc tattgaagaa ggcgtgccgc
     5101 gcttcctgaa aggtcagggc cagggctgga tcaccgcaga gtacggcatg ctgccacgtt
     5161 ctacccacac ccgtaacgct cgtgaagcgg cgaaaggtaa gcagggtgga cgcacaatgg
     5221 aaatccagcg tctgatcgcc cgtgctcttc gcgcggcagt agatttgaaa gcgctgggtg
     5281 agttcaccat tacgctggac tgcgacgtgc ttcaggctga tggtggcacg cgtaccgcgt
     5341 cgattacggg tgcctgcgtg gcgctggcag atgcgctaca gaagctggtg gaaaacggca
     5401 agctgaaaac caacccgatg aaagggatgg tagccgcagt ttctgtcgga attgtgaacg
     5461 gcgaagcggt ttgcgatctg gaatacgttg aagactctgc cgcagagacc gacatgaacg
     5521 tagtgatgac cgaagacggg cgcatcattg aagtgcaggg gacggcagaa ggcgagccgt
     5581 tcacccatga agagctactc accttgttgg ctctggcccg agggggaatc gaatccattg
     5641 tagcgacgca gaaggcggcg ctggcaaact gatttttaag gcgactgatg agtcgccttt
     5701 tttttgtctg tagaaaagta agatgaggag cgaaggcatg aaaccatatc agcgccagtt
     5761 tattgaattt gcgcttggca agcaggtgtt aaagtttggc gagtttacgt tgaagtccgg
     5821 gcgcaaaagc ccctatttct tcaacgccgg gctgtttaat accgggcgcg atctggcact
     5881 gttaggccgt ttttacgctg aagcgttggt ggattccggc cttgagttcg atctgctgtt
     5941 tggccctgct tacaaaggga tcccgattgc caccactacc gccgtggcgc tggcggagca
     6001 tcatgacctt gacctgccgt actgctttaa ccgcaaagag gcaaaagacc acggtgaagg
     6061 cggcaatctg gttggtagcg cgttacaagg acgcgtaatg ctggtagatg atgtgatcac
     6121 cgccggaacg gcgattcgcg aatcgatgga gattattcag gctaatggcg cgacgcttgc
     6181 tggcgtgttg atttcgcttg atcgtcagga acgggggcgc ggcgagattt cggcgattca
     6241 ggaagttgag cgtgattaca actgcaaagt gatctctatc atcaccctga aagacctgat
     6301 tgcttacctg gaagagaagc cggaaatggc ggaacatctg gcggcggtta aggcctatcg
     6361 cgaagagttt ggcgtttaaa gaaactcgcc ggatgaaaag tcatccggcg tcatattact
     6421 gcaactgtgc cgcaattagc ggccagcggg cgtcaaaatc atccgtcggg cggtatttaa
     6481 attcgctgcg gacaaaacgt gacagcatac cttcacagaa ggccaggatc tggcttgcca
     6541 gcagggtttc atcggtggtg taaccttcac cctcacgcat tctcttttca cgcaatacct
     6601 gacgcagctg cgcttcaata cgctcgaaca gctggttgat gcgcccttgc aggcgatcct
     6661 gttcaaacat tagcgcatga ccagtgagga tgcgggtcag gccaggatta cgctcaccaa
     6721 aaccgagaag cagcaacaca atcagacgca ggcgcgctgt ggtgtctttc tcatctttca
     6781 gaatcaggtt gatgcgagta atcaggctat cttcgataaa ctcaatcagg ctatcgaaca
     6841 tgcgggtctt actggggaag tggcgataca gtgccgcttc ggaaacgccg acagaggcgg
     6901 ccagttttgc cgtcgtgata cgttggcttc catcgctgga ttccagcatc agcgccagag
     6961 actgaagtat ttcctcgcga cggttccttt tcgcagtttg tttttctgcc atgttacaaa
     7021 atacccctga aaataagcac ttgccaggcg gcacccacgc tatgaccgca aacgaaatgt
     7081 ttgcggctat gttatgacgt tattcggatg cgtatgtgtt actgacgacc agagtgacca
     7141 aagccgcctt caccgcggtc ggtggcgtcg aaatcttcca ccagattaaa ttcagcctgt
     7201 actaccggaa caaaaatcat ctgggcgatg cgttcgccag gttgaatggt aaagctgtcc
     7261 tgaccacggt tccacacgga aatcatcaac tggccctgat agtcggaatc gatcaatcct
     7321 accaggttac caagcacgat accgtgctta tgtcccaatc cggagcgcgg cagcatcatt
     7381 gccgccagtg aaggatcggc aatatgaatc gccagcccgg tcggaaccag cgtagtgtca
     7441 cccggagcca gttctacggc gtcgtcgaga caggcacgca ggtcaagtcc ggcagagcca
     7501 gaggtggcat aagtcgggag cggaaattcc ttcccaacgc gcgggtccag aatcttaacg
     7561 tcgatttttt tcatcataac gggtcacgat ctcgtcgagt aatagttggc caaggagctc
     7621 tttgcgctca agcggtaaga ctttatctcc gtcctgccag aaaaggtgta atgcgttgtt
     7681 ttcgctgtta aatccttgag ttggctggga aacatcgttc gcgcagatca gatcaaggtt
     7741 tttacggata cgtttttgcc gggcgtattc ttccacatta tttgtttcgg cggcaaatcc
     7801 aacgacgtag ggtcgatggt cttttagtgc ggcaacgcct gcgacgatat cggggttttt
     7861 aaccattttt attgttaatt catcaccctg cgtggcctgc tttttgattt tttctggggc
     7921 cacggtagct gcgcgataat ccgccacggc ggcgcagccg ataaaaatat tttgctgctg
     7981 tacagaagca ttcacggcgg cttccatttc cagcgcggtc atcacatcaa cacgtttaac
     8041 aaacggtggc gtcggtagtg aaaccggacc tgataccagc gtgacgttcg cgccacgacg
     8101 ggcagcggcg gcggcgatag caaaacccat cttgccggag ctgtgattag agatataacg
     8161 cacgggatcg agcggttcac gcgtcggacc ggcggtaatc ataatgttca gatgtttcag
     8221 gtcgttgacg ggcgaaaaat gcgccaccgc catatccaca atggttaacg gatcgagcat
     8281 tcgcccagga ccgatatcac cacaagcctg actgccactg tctggccccc agatgagcaa
     8341 accacgggaa gcaagcccct ctaaattatg ctgcgtggca gcggcacggt acatctgctg
     8401 gttcatggcg gggagcacgg ctacaggcgc aggtgtagcc agacaaatcg tcgataccag
     8461 gtcattcgcc attccggcag caacacgggc aatcaaatct gccgtggcag gggcgagaat
     8521 cactaaatca gcccatttac ccagctcaat atggcccata gcggcttcgg ctgccgggtc
     8581 cagcaaactg tcggaaacgg gataaccaga aaccgcctgc aagctaagtg gggtgataaa
     8641 ggcttttgcc gcttcggtca tggctacgcg gacgtcggcc ccgcgatcgc gcaaacgacg
     8701 caccagttca ggggttttat aggcagcaat accgccgcta acgccgagaa cgattttttt
     8761 accggccagg ctcatgatga tttttcctgt tgggtgacac cagaagttgg cgattttatc
     8821 acaatacttt tgttgtcgtg ccttcacctg agaatcactt tgcgaggcgc tttccaggat
     8881 tgaaaactgg ccgtcgattt aacggaacgg ctatgacagg atgcgagcac cacaaaggag
     8941 gtgaaggtga aaaacaattc acagctgttg atgccgcgcg aaaaaatgct gaagtttggt
     9001 attagcgcct taacggatgt cgagctgctg gcgctatttc tgcgtaccgg aacgcgcggt
     9061 aaagatgtat taaccctggc aaaagagatg ctggagaatt tcggctctct ttatggcttg
     9121 ttaacctctg aatatgaaca atttagtggc gttcatggaa ttggcgtggc gaaatttgcc
     9181 cagttaaagg ggattgctga actggcgcgg cgttactaca acgtgcggat gcgtgaagaa
     9241 agccctttac tcagcccgga gatgacgcgg gaatttttac aaagccagct tacgggtgag
     9301 gagcgggaga tctttatggt gatctttctc gactcacaac accgggttat aacgcatagc
     9361 cgtctttttt ccggcacgct aaaccatgtt gaagtccatc ctcgggaaat tatccgcgaa
     9421 gcgataaaaa taaacgcctc ggcgctgatc cttgcacata atcacccttc gggttgtgct
     9481 gaacccagta aagcggataa actcattact gaacggataa taaagagttg tcagttcatg
     9541 gatttacgcg tgctcgacca tatcgtgatt gggcgtggag agtatgtttc ttttgccgaa
     9601 cgcggctgga tttaacccgc tatgcgcgat ccttcgggat ctttgtctgt tcgggacttg
     9661 agcacatcgc tgagtcagcg tatactacgc cacctttgag aatctcgggt ttggcatttg
     9721 ggcctggcaa tcgagagttc acatagaact gcgatgactg ggctgtaaag cctgacgagg
     9781 cgccaatacc ccatacgaag ctcgagctaa tttgattttt ggagaataga catgtcccga
     9841 gtctgccaag ttactggcaa gcgtccggtg accggtaaca accgttccca cgcactgaac
     9901 gcgactaaac gccgtttcct gccgaacctg cactctcacc gtttctgggt tgagagcgag
     9961 aagcgttttg tcaccctgcg cgtatctgct aaaggtatgc gtgtaatcga taaaaaaggc
    10021 atcgatacag ttctggctga actgcgtgcc cgtagcgaaa agtactaagt acttagagga
    10081 aataaatcat ggctaaaggt attcgtgaga aaatcaagct ggtttcttct gctggtactg
    10141 gtcacttcta taccactacg aagaacaaac gtactaagcc ggaaaaactg gaactgaaaa
    10201 aattcgatcc agttgttcgc cagcacgtga tctacaaaga agcgaaaatc aaataattct
    10261 cgctttgatg taacaaaaaa cctcgctccg gcggggtttt ttgttatctg cttgccccca
    10321 tattgactgc atctgttcat tcctggagat gctatgcctg aattacccga agttgaaacc
    10381 agccgccgcg gcatagaacc gcatctcgtt ggtgcaacca ttcttcatgc agtggtgcgc
    10441 aacggacgct tgcgctggcc ggtttcagaa gagatctacc gtttaagcga ccaaccagtg
    10501 cttagcgtgc agcggcgggc taaatatctg ctgctggagc tgcctgaggg ctggattatc
    10561 attcatttag ggatgtctgg cagcctgcgc atccttccag aagaacttcc ccctgaaaag
    10621 catgaccatg tggatttggt gatgagcaac ggcaaagtgc tgcgctacac cgatccgcgc
    10681 cgctttggtg cctggctgtg gaccaaagag ctggaagggc ataatgtgct ggcccatctt
    10741 ggaccggagc cgcttagcga cgatttcaat ggtgagtatc tgcatcagaa gtgcgcgaag
    10801 aaaaaaacgg cgattaaacc gtggctgatg gataacaagc tggtggtagg ggtagggaat
    10861 atctatgcca gcgaatcact gtttgcggcg gggatccatc cggatcggct ggcgtcatca
    10921 ctgtcgctgg cagagtgtga attgttagct cgggtgatta aagcggtgtt gctgcgttcg
    10981 attgagcagg gtggtacaac gctgaaagat tttctgcaaa gtgatggtaa accgggctat
    11041 ttcgctcagg aattgcaggt ttacgggcga aaaggtgagc cgtgtcgggt gtgcggtacg
    11101 ccgattgtgg cgactaaaca tgcgcagcgg gcaacgtttt attgtcggca gtgccagaag
    11161 taattcatgc gcgccggatc gcataccatc cggcataaac gctacgctaa cttcgccatc
    11221 agcgcctgat ggacattctc cggcaggaaa tgggtgacat cgccctgatg gcgcgccacc
    11281 tctttcacca acgatgaaga gataaacgac cactctttcg acggcatcag aaacacactt
    11341 tccagttccg gcattaagtg gcgattcata tgcgccagct gcatttcata ttcaaaatcc
    11401 gccaccgcac gcaggccacg aatcagcacc gtagcgtgtt gattacgggc gaagttcgcc
    11461 atcaaatcac taaacccaac tacttccacg ttccccagat gcgcggttgc ctgctgtgcc
    11521 agcgccacac gctcttccag ggtaaacatc ggttttttac tggggctggc ggcaatcgcc
    11581 agaataacgt gatcgaacat ttgcgtggcg cgcgtcacga tatcgatatg accattggta
    11641 atgggatcga aagtacccgg ataaatcgcc cgtttttgca taacaacctc aatgcgtttt
    11701 cggtggcagg taaggttcca gcagttgaag caggcgctgt agcgcgccct ggttttgata
    11761 cagtacttca acggcatgac ggccatagaa actacggtaa tcggcgtcgg tgagtaaaga
    11821 ggaaacctct tttgcaagcg tagtggcatc ggtaacggta atcagcccgc ttgcctgctc
    11881 cagccgcgcg caaatgtctt taaaattaaa agtatgcggc cccatcaata ccggaatagc
    11941 gtgtgcggca gcttccagcg gattatgccc accacgttca accagtgaac cgccaacaaa
    12001 ggcgagatcg gcaatgccgt acagtaacat caactcgccc atcgtatcgc caaccacaac
    12061 ctgcgtgctg gtggagggga cttcccctga agagcgtgtg atatagctta gtccagcctg
    12121 gcggacaagg ttaatcgcat ccgggaagcg ttctggatga cggggtacca ggatgagcaa
    12181 taaattcggg aattgctgta acaatacctg atgtgcggcg atcaccacac tctcttcacc
    12241 ttcgtgagtg ctggtggcaa tccataccgg gcggtgtggt gcccactggc ggcgcagcgt
    12301 cacggcttta gcagccaact gcggcgttac agaaatatcg aatttcaggc taccggtaac
    12361 ggttacctgg ttattttttg cgcccagcgc cacaaaacgt gcaccatctt cttcattttg
    12421 ggcggcaatc agcgtaatac gacgcagcaa gcgacggacg aatttaccca gtttggcata
    12481 acctgcggcc gagcgggcag agagtcgcgc gttcgcgatc accagcggaa ttttacgttt
    12541 atgtagcgcc gcaatcaggt taggccatag ttcggtttcc ataatcaaca ccagtttagg
    12601 gtcgacttta ttcaggaaac gattaagtgc atcgggcagg tcatacggca gataaacgtg
    12661 ctgaacatcc ttcccgaaag ccgattgtac gcgctccgaa ccggttggcg tcatggttgt
    12721 aacggtaatc ggtaaatcag gataacgatg acgcagcgcg cgcaccaacg ggatcgccgc
    12781 cagagtttca ccgacggaga cggagtgcag cataatgccg cctggtttta gcggatggcg
    12841 gtaaaaaccg taacgttcac cccagcgttt tcgataggcc ggagccttac gtccgcgcac
    12901 ccagagccgt atccagatca gcggctgaat aaggtagaga agggcggtgt aaagcaattc
    12961 gagcatagta aatagctgac ttatggatgt gctggggatt ctatgtattt agctgtggct
    13021 ttaccattac ttttcccgtt tttgacttaa atagcaattt acccgccaat tcttgctgaa
    13081 catctatact ttttttgtat ttttatgcga ttcattgatt tctgctgtac gtgcaggaag
    13141 gaatcgcaga gcaacagtga aagaatatgg ttgctaatga ttttatttga ttgatttctt
    13201 tattgaaaat caacaatgtg cttaaaaatc attcgggtcg gattgccgcg aaagaagtga
    13261 tacactagcg cgtcaatatc agtgcggtat cagtattcag gtagctgttg agcctggggc
    13321 ggtagcgtgc tttttctgct tcaccttaat cggataatct caacaaaaag agttacttgt
    13381 ggataagcta tttcgaagaa ttttgctcat taagatgcgt tttcatgggg atatgttatt
    13441 aactactccc gtcattagtt cgctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct
    13501 gctttatcag gacaccatcc cgatcctgtc tgaaaatcca gagattaacg cgctctacgg
    13561 cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc aacttttttc atctcatcaa
    13621 ggtattacgt gccaataagt atgaccttat cgtcaatctc accgatcaat ggatggttgc
    13681 tatactggtt cgcttattaa atgcccgtgt gaaaatttcc caggattatc atcatcggca
    13741 gtctgctttt tggcgtaaaa gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt
    13801 ggaaagtaac ttatccgtgc tgaccccatt gcgagttgat tcgttggtga agcagacaac
    13861 catgagttac ccgcctgcaa gctggaaacg tatgcgtcgc gaacttgatc atgctggtgt
    13921 tggacaaaat tatgtggtta tccaacctac ggcgcggcaa atcttcaaat gctgggacaa
    13981 cgccaagttt tccgctgtga ttgatgcctt acatgctcgt ggttatgaag ttgttctgac
    14041 gtccggccca gataaagacg atctggcctg cgtcaatgaa attgcgcagg gatgccagac
    14101 gccaccagta acggcgctgg ctggaaaggt gaccttcccg gaacttggtg cgttaatcga
    14161 tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg catattgccg ctgcagttaa
    14221 tacgccgctg atatcgctgt ttggtgcgac agaccatatt ttctggcgtc cctggtcaaa
    14281 taacatgatt caattctggg cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga
    14341 ccgaaatgag atgtatcttt cggttattcc ggcggcagat gtcattgctg ctgtcgataa
    14401 attactgccc tcctccacga caggtacgtc gttatgatcg ttgctttttg tttatataaa
    14461 tattttccct ttggcggttt gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc
    14521 cgaggtcatc atgttcgggt ttatacccag tcgtgggaag gcgaataccc tgatgtattt
    14581 gaactgatca aagtgccggt taaatcgcat accaatcacg ggcgcaatgc ggagtatttt
    14641 gcctgggtgc aaaaacattt acgcgaacat cccgtcgata aagtcgttgg attcaacaaa
    14701 atgccggggc tggacgttta ttatgccgct gatgtttgtt atgccgagaa agtcgcgcag
    14761 gaaaaaggct ttttctatca cctgacgtca cgttatcgcc attatgccgc ctttgagcgg
    14821 gcaaccttcg aacagggcaa gccgacacag ctgctgatgc tgacagataa gcaaatcgcc
    14881 gatttccaga aacattatca gactgaagcg gagcgttttc atattctgcc accggggatt
    14941 tatcctgatc gtaaatatag ccagcagcca gctaatagcc gtgaaatctt ccgtaagaag
    15001 aatggaataa ccgaacaaca atatttattg ttgcaggtcg gttcagactt cacgcgtaaa
    15061 ggtgtcgatc gttccattga agcacttgct tcgttaccgg attcgctgcg ccacaacaca
    15121 ttgctatatg ttgttggaca ggataaaccg cgaaaatttg aggcgctggc agaaaaacgc
    15181 ggcgtgcgca gtaatgttca cttcttctcg gggcgcaacg atgtctcgga attaatggcg
    15241 gcggcggatt tattactgca tcctgcctac caggaagcag cgggaattgt gctactggaa
    15301 gcgattactg caggattacc ggtactaaca acagccgttt gtggctatgc gcattatatt
    15361 gtcgacgcca attgcggcga ggctattgct gagccattcc gccaggaaac attgaatgag
    15421 attttacgca aagcgttaac gcaatcttca ttgcgccagg cctgggcgga aaatgcgcga
    15481 cattatgctg atacacaaga tttatacagt ctgccagaga aagcggcgga tatcataacg
    15541 ggtggtctgg atggttgaac ttaaagagcc gtttgccacg ttatggcgcg gtaaagatcc
    15601 ttttgaggaa gttaaaacct tgcagggtga ggtttttcgt gaactggaaa ctcgccgcac
    15661 tctgcgattt gaaatggcgg gcaaaagcta ttttctcaaa tggcatcgcg gcacgaccct
    15721 gaaagagata atcaaaaatt tactctcatt gcggatgcca gtattaggcg cagaccgcga
    15781 atggagtgcg attcatcgac tgcgggatgt cggcgttgat actatgtatg gggtggcatt
    15841 tggcgaaaaa ggtattaatc cgctgagcag aacctcgttt attattaccg aagatctgac
    15901 cccaaccatc agtctggaag attactgtgc tgactgggcg accaacccac cagatgttcg
    15961 cgtaaagcgt atgcttatta agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg
    16021 cattaaccac cgtgactgtt atatctgtca tttcctgctg cacttgcctt tttccggtaa
    16081 ggaagaggag ttaaaaattt cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt
    16141 tccacgtcgt tggcgcgata aagatcttat agggctttat ttttcttcaa tgaatattgg
    16201 cctgactcag cgggatatct ggcggtttat gaaagtgtat tttgccgccc cgcttaaaga
    16261 cattctcaag caggaacaag gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag
    16321 ggaaagaacg attcgaaaat cgttgtaatt tccggagaga aatatgagtg cccactattt
    16381 taatccacaa gagatgatca ataagacaat catcttcgat gaaaggccag cggcgtcagt
    16441 ggcatcatca ttccatgttg cttatggcat tgataaaaac tttctttttg gttgtggtgt
    16501 ttcaatcacg tcagttttgt tacataacaa cgacgtgagt tttgttttcc acgtttttat
    16561 tgatgatatc cctgaagccg atatccagcg tttagcccaa ttggcgaaaa gctatcgtac
    16621 ctgtatccag atccatctag taaattgtga acggcttaag gcattaccga cgaccaaaaa
    16681 ttggtctatt gccatgtatt tccgttttgt aattgcagat tactttattg atcaacaaga
    16741 taagatcctt tacctggatg ctgatatcgc ctgtcaggga aacttaaagc cgctgataac
    16801 aatggatctt gccaataacg ttgctgctgt tgttactgaa cgcgatgcta actggtggtc
    16861 gttacggggt caaagtctgc agtgtaatga acttgaaaag ggctacttta attcaggtgt
    16921 cctgttaatt aatacactag cgtgggcgca ggagtccgtt tctgctaaag cgatgtcgat
    16981 gcttgctgat aaagccatcg tttcccgttt cacctatatg gatcaagata tccttaatct
    17041 tatcctgtta gggaaagtta aattcattga tgctaaatac aatacgcaat ttagtttaaa
    17101 ttatgaatta aaaaaatcat ttgtttgtcc aattaatgat gaaaccgtat taattcatta
    17161 tgtcggcccg acaaaaccct ggcattactg ggccggttat ccaagtgcgc aaccttttat
    17221 caaagccaaa gaagcatcgc cctggaaaaa tgaaccgtta atgcggccag ttaactcaaa
    17281 ctatgctcgt tattgcgcca agcataattt taaacaaaat aaaccaatta acgggataat
    17341 gaattatatt tattattttt atttaaagat aataaaatga aattgagaaa agttaaatga
    17401 atgaatttat aaaagaacgg ttttcgtatt tagcagataa taaaaaagaa aacgccccag
    17461 agctaaatgt ttcctacggt atcgataaga attttttgta tggtgctggc gtttcaattt
    17521 cttccgtttt gattaataat tcagatatta attttgtctt tcatgttttc actgattatg
    17581 tggatgatga ttatttaaag tcatttaatg aaacagcaaa acaatttaat acctcaatta
    17641 ttgtatattt aattgacccc aaatactttg ctgatctgcc gacgtcacag ttttggtcat
    17701 acgcgacata cttcagggta ttgtcttttg aatatctgag tgaaagtatt tccacactgc
    17761 tgtatctgga tgccgatgtt gtttgtaaag gaagcctgaa acctctcaca gaaattatat
    17821 ttaaagatga gtttgctgcg gtcattcctg acaatgatag tactcaggcg gcatgtgcaa
    17881 aacgcctcaa cattcccgaa atgaatggac gttatttcaa tgcaggcgtt atctatgtca
    17941 atcttaaaaa atggcatgaa gcaaatttga caccgtattt actcaaactt ttacgagggg
    18001 aaactaaata tggctctctt aaatatttag atcaggatgc gttgaatatc gcatttaata
    18061 tgaataatat ctaccttgcg aaggattttg atactattta taccctgaaa aacgaacttt
    18121 atgatcgtag tcatcgaaag tatcagcaaa ccattaccga taaaacagtg ttgattcact
    18181 atacagggat aactaaacca tggcatagct gggctggata tccgtctgca tcatacttta
    18241 atatcgcgcg tgaacaatct ccctggaaga aatatcctct taaagaggcg cggactgttg
    18301 cagaaatgca gaaacaatat aagcatctgt ttgcccatgg tgagtatata aaaggcataa
    18361 cttcattaat taagtacaag cttaagaaat aaattactct tatgattaca agtatacgtt
    18421 atcgcggctt ctcattttat tacaaagata atgataataa atataaagaa atctttgatg
    18481 aaatactggc ctataatttt aaaacagtaa aagtattatg taatattgat gataccaaag
    18541 tatcgttgat tgatacgaaa tatggtcgct atgtttttaa agtctttgct ccgaaaacga
    18601 aaaggaatga acgtttttta aaatcttttg taaaaggtga ctactatcag aatttgatcg
    18661 ttgaaaccga tcgtgtcaga agtgcaggtc ttacttttcc taatgatttt tattttctgg
    18721 ctgagcgtaa aattttcaac tatgccagtg tttttatcat gcttatcgag tatgtcgagg
    18781 gcgtagagct caatgatatg ccgattattc cagaaaatgt taaagcagaa attaaggctt
    18841 cgatggaaaa attgcatgcc ttaaatatgc tatcgggcga cccacaccgg ggtaacttta
    18901 tcgttagtaa agatggtgtc aggataattg acctatccgg taaaagctgt acggcagagc
    18961 gtaaagctcg tgatcgtctg gccatggaac gtcacctggg tattgccaac gaaataaaag
    19021 attatggcta ctattcagta atctacagaa ccaaactacg caaatttatt aaaaaattaa
    19081 aaggcaaagc gtaaaccaca cagtcaaaac ggaaccaaca tggatttatt agcagagagt
    19141 attactgaag tcgctgtctc tggggaaatt gctaacaccg atcgtgtgtt aaatatcgct
    19201 tacggtattg accgtaactt tttatttggt gcggcagtat ctatgcaatc agttgttatg
    19261 cataacccgg accttgcggt taagtttcat ctctttaccg actacattga tgaagattat
    19321 ctacaacgtg ttaatgcttt taccagcaaa aatgctaacg ttgaagtaat aatttataaa
    19381 gtctccaatg cctttattga tatcttcccc agcctgaaac agtggtctta tgcaacattc
    19441 ttccgtttag ttgcgttcca gtatttgagt gaaactattg aaaatctgtt atatatcgat
    19501 gctgatgtca tctgtaaagg ctcattagct ggattgcttg atattaattt tgatgaggat
    19561 aagttcgcag ctgttattaa agatgtgcct tttatgcagg aaaaaccagc gaagcgtctg
    19621 gctatagagg gacttccagg gaattatttc aacgccggtg tagtatatct gcagcttgaa
    19681 gcatgggcga aaaatgattt tatgaataaa gccattgcta tgctggcaag tgacccgcag
    19741 cacacgaagt ataaatgcct tgatcaggat attttaaata ttctgttctt tggtcattgt
    19801 aattttatta gcggcgatta tgattgcttt tatggcattg actatgagtt aaaaaataaa
    19861 agcgatgaag attataaaaa gaccattacc gatgatacta agctgattca ttatgttggc
    19921 gtaacgaagc cctggaacga ctggacgaat tatccctgcc agaagtattt taatgaggct
    19981 tatcaggctt cttgctggaa tgatgtggcg tttattccag ccacgaatga aaagcagtat
    20041 caagtgaaat atcaacatgc aaagaaaaat ggtgatacgt ttaacgcttt tatttacttc
    20101 attaaattta aattaaataa gtataaaaga aaactatttg gataataata aaaatcatgg
    20161 atgtctgagt tacaataata attaaggatg agtactatca tattttatta gttcaggcaa
    20221 gagaacacga tgagcaatga ttacccttta gtatctataa taataccgac gtataattca
    20281 tctgattaca ttactgaaac tctaacgaaa ttagaaaaac aaacttaccc aaattttgaa
    20341 attgttattg ttaatgatgg ttctaaagat aacacatcaa acgttttgag agagtatggg
    20401 ttaacccact ctcgattaat tattgtcaat aaagaaaatg gcggtgtttc gtctgccagg
    20461 aatacaggta tccgcaaggc gcaaggacag tttatatgtt ttatggatga tgatgatgag
    20521 atagatccta actatctgct gaagatgtat accagacaac atgagacggg aggggatgcg
    20581 atttattgtg ggctttatgg ccattatata aaagatggtg ttacttactc acctataaat
    20641 acagagttta atgaaggatc tttacttttc gacttttttt ataaaaaggt tagattccat
    20701 atagggtgct tgtttataag aaaacaactt ctggaagaga ataatctttt ttttgatgaa
    20761 gatttacgac taggagaaga tcttgatttt atctatcgac tgctaattac atgcgatatg
    20821 tatgcggttc catattatat gtataagcat aactatagag aaaattcctt aatgaactca
    20881 tgtagaacca tcactcatta tcgacatgag tcatttgcgc acgaaagaat ctactcttct
    20941 gtgatgcagt tatacaaagg taaccggaaa gaagaaattc atacattatt gagtcaaaat
    21001 agagcttatc ataaaactcg ttatttatgg aatgttctac ttaatggtga ttttaaacta
    21061 ttgaatcaat tagttgaaag caatgaaaaa gaattaagat attgtaatct tcctggcaag
    21121 agagataaga gacgagcaaa aatattagca tcaaaaaatt atattctctg gaggatggta
    21181 agactggtaa atagaaaaaa gaataaacgt tagatcttgc attaaataac ccatcctcgt
    21241 agcataggtt gaaattatat gtcgttttgt tggaatgaaa ttaattctgg tataaagtct
    21301 ttaattctta tattatgtat tttttcttta atgactttgt ctttatggga tgatgttgca
    21361 acaaagtttc ttcatgcagc tggaattata tctgcattgt attttcttgc gacaccaaaa
    21421 aaaacaataa ctaataatcc tactttgtta attttcatct cattatgtct tttgggtatc
    21481 gtaaatatca tctggtattc acattataaa gtttcaggct ctgtttatac caatgcatat
    21541 cgtggcccaa tggaaactgg aaaaattgcc ttgtgtagcg cttttatttt cttagttctt
    21601 tttgctaaaa atgagatgag aacaaaaata aaatttggga aactaattct gttcgcatcc
    21661 ctggcaacgc agttactttt ttttgcgcat gccatgtggc aacatttcta tttaaacgtc
    21721 gaccgtgttg cattatcagc ttcccacgct acaacagcag gctacatcat cctttttcct
    21781 tctttactgg catcaattct cattttaaaa tccgacttta gacataaaac aacattatat
    21841 acaattaact tcatgcttag cttatgtgct gtcatagtaa ctgagacgcg tgcagccata
    21901 ttagtgtttc cattctttgc gttaatatta atcgtaatgg atagttatat taataagcga
    21961 attaattata agttatattg ttttattgcg attgcattat tagcaggtgt attttctttt
    22021 aaagatacat tgcttatgag aatgaatgac ttaaataacg atttagttaa ttattcgcat
    22081 gataacacca gaacttcagt cggtgcccgt ctggcaatgt atgaagttgg cttaaaaaca
    22141 tattctccaa taggacaatc actggaaaaa cgtgcagaaa aaatacatga gctagaagaa
    22201 aaagagccta gattgagtgg cgctttacct tttgtagatt ctcatttgca taacgatctc
    22261 atagatacgt tatcaacgcg tggtattcct ggagttgtat taacaatttt agcattttca
    22321 gcaatactca tatatgcctt aagaactgct aaagaacctt atattttaat cttgcttttt
    22381 tcactactgg tagtaggcct aagtgatgta atactctttt ctaaaccggt tccgactgct
    22441 gtgtttgtca ccataatatt gctttgtgct tattttaaag cacaatcaga ccaatattta
    22501 ttagataagt aatgaatata tgaaataaaa tcagcaagga ggctgatttt atttcaaaat
    22561 tatctattaa ggggttgtaa tttaaatcat agcagcgttt tcagcgtttt cttcaatgaa
    22621 tcgcttaaca gcatttgctg tcaattgaga caactcattc cccggagccc tacaaaccat
    22681 ttgattcttc ccatacccac caattaatcc cggatcggtt ggtccataaa ccgtgatatt
    22741 gggtctatcc agtgccgccg ttaaatggct taaccccgta tccaccgaca ctacaaattt
    22801 agccccggcc agcacgcggg caacgccttc cagactcatc ttcggcaata cttcaacata
    22861 atcaaatcct tccgccagtc gtttcgcccg ttcttcctca tgcggcgcgc cccacggaag
    22921 tttaatccgt attcctgaat cagccagtaa accaatcaat tctcgccagt gttcttccgg
    22981 ccagtgttta tcatcacggg tcgtcgcatg aagaaatacg gcatattcgc cagcatctgt
    23041 aggcaggttc gtcagaaaat gctgtgcgat agcataatcg ccctgggttt gcggtttgct
    23101 atagcccaaa cttttggcaa acagttcgcg ggtgcgttct acggcgtgct gctgttttgc
    23161 aatatgatgc ttacaattgt aaaacaggct ggctaaaggt tcgcgagcgg tttgccagtc
    23221 caatccatgc tttacgccat gcgccagacg tgtcaccagt gccgcgcttt ttaccagccc
    23281 ctgagcgtcg ataactgcgt catagttctc tgcttgtagc gcttcacgaa acgctttgcg
    23341 ttcagctttt atgggggccg agaaccaggc tttacgccag cgacgtattg ccacaggaat
    23401 aactcgctca acggcagcgt gccaggaagg aatctgtgcg aacccttctt ccaccaccca
    23461 gtcaaactta atccctggga ttgcctgctg ggcatcagtg agtgcgggca acgtatggag
    23521 aacatcgccc atcgacgatg ttttaacgat caaaacccgc atccgtcagg cttcctcttg
    23581 taacaatagc gtgttgagtt cttccagtac gcgctgggga gtaatgtcga tcaagctctg
    23641 gtgataaccc tccgcagcgt cacctttacg cactttgtga tagccggtaa tcagacggat
    23701 cacgcgcgct ttatgggata gcggcggtgt gaagtccggg ctacttggac catacagggc
    23761 aaccagcgga cgattgagcg ccgccgcaac gtgcatcagg ccagaatcgt tagtgacaat
    23821 ggctttacag gctgcaatca ggataaccgc ttgatcaagc tgtgtttccc ccgccaggtt
    23881 ccgacaccat gcctgctgct cggtattcaa agcggcaaga atctcattgc ccgcttcatg
    23941 atctttcgca gagccaaaca gaaccacctg ataaccttca tcaatcagct gctttgccag
    24001 ttccgcatag tggtagtgtg gccagcgttt tgccggacca aactccgcac ccgggcaaaa
    24061 gccaatcatc ggacgttctg atgaaagcga aaattgatta caggtatatg atttttcacc
    24121 ttcgctcacc tgcaactgcg gccataacaa tggctgcggc agatcttgtg cggtacgcat
    24181 aatgcctttg tcataggcca gcgcgacata gcgttccacc attagcggcc aggcttcttt
    24241 atcgagcacg cgtacatcgt tgagtaaacc gtagcgcatc tcgccgcgcc agccggtgcg
    24301 atgaggaata cccgcgaaga aaggcactaa tgcagatttg aaggagttgg gtaagacgta
    24361 ggcgcggtcg tagcgctttt cacgcaggct atgacccagt ttgcggcgtt cgccgatttc
    24421 cagcgctccg tgaccgagag gcatagggat agcttcgtta acttccggca tccgcgataa
    24481 taatggacgg caccatgccg gtgccatcac atcgattatc gcctggggat agcgcgcctg
    24541 gagcgtgcga tagagacttt gcgacatcat catgtcgcca acccaagacg ggccgatcac
    24601 cagtattttc atgcagagct cttatgcgtc gcgattcagc caggccatgt attccgttac
    24661 accttcagca acggttttga acggtttgtc gtaacccgcc gcgcgcagat ttgtcagatc
    24721 tgcctgagtg aacgcctggt agcggccttt cagcttatcc gggaacggaa tgtattcgat
    24781 ctggcctttc ttgtgataag ccagagtagc atctgctacg gcctggaagg attccgcacg
    24841 accagtaccg aggttgaaga taccggaaac gccattttcc aggaaccaca ggtttacatc
    24901 tgccacgtcg cccacataga cgaagtcacg tttgaagttc tcgctacctt caaacagctt
    24961 cggtgattca ccgttgttaa gctgagtgtt gagatggaaa gcgacgctcg ccatgctgcc
    25021 tttatggcct tcacgcggtc cataaacgtt gaaatagcgg aagccaacaa tctgcgagtt
    25081 cgcttcaggc aggatttggc gaacatattc atcaaacagg aattttgagt aaccgtagac
    25141 gttcaacggt ttttcgtact cgcgggattc aataaagtcg gaggtgcgtc cgccgtaggt
    25201 ggctgcggaa gaagcgtaca ggaacgggat ttcgcgctcc aggcagtagt gcagcagctc
    25261 cttggagtat tgatagttgt tatccatcat atacttgccg tcccactcgg tggtggaaga
    25321 gcatgcgcct tcgtggaaaa tcgcttcgac atcgccgaac tcttcgccag ccataatctg
    25381 gatcaggaag tcttccttat ccatatagtc cgcgatatcc agatccacca ggttcacaaa
    25441 cttggtgccg tctttcaggt tgtccaccac cagaatatcg gtgatgcctt tatcattcag
    25501 ggccttaacg atgttgctgc cgataaagcc cgcgccgccg gtaacgatga tcataactgt
    25561 aaccttcgaa ttatggagtc agagacaatc tcagacacga atacttctat catatcacta
    25621 tacggcgttg gcttcagcca ttcgccaaaa gtgagattga tggacgttat ttatcctgaa
    25681 ttttgcagaa gtgttaacgc gtcatctcgt cgcgacgtat aagtttgggt aatatgtgct
    25741 ggaatttgcc ctgtctggag aatcgcaatg cgtggagaat tttatcagca gttaaccaac
    25801 gatctggaaa ccgcacgggc ggaagggttg tttaaagaag agcgcattat tacgtctgcg
    25861 cagcaagcag atatcactgt ggctgatgga agccacgtca ttaacttttg tgccaacaac
    25921 tatctcgggc tggcgaatca tccggatctg attgcggcag caaaggcggg aatggattct
    25981 cacggtttcg gcatggcttc ggtgcgtttt atttgcggca ctcaggacag ccataaagag
    26041 cttgaacaaa aactggcggc attcctgggg atggaagatg cgattctcta ctcttcctgc
    26101 tttgatgcta acggtggact gtttgaaacg ctgctgggtg cggaagacgc cattatctcc
    26161 gacgcactga accacgcgtc tattattgat ggtgtgcgtc tgtgcaaagc taaacgctat
    26221 cgctatgcca acaacgatat gcaggagctg gaagcacgtc tgaaagaagc gcgtgaagcc
    26281 ggtgcgcgtc atgtgctgat tgccaccgat ggtgtgttct caatggacgg cgtgattgcc
    26341 aacctgaaag gcgtttgcga tctggcagat aaatacgatg ccttggtgat ggtggatgac
    26401 tcccacgcgg tcggttttgt cggtgaaaat ggtcgtggtt cccatgaata ctgcgatgtg
    26461 atgggccggg tcgatattat caccggtacg cttggtaaag cgctgggcgg ggcttctggt
    26521 ggttataccg cggcgcgcaa agaggtggtt gagtggctgc gccagcgttc tcgtccgtac
    26581 ctgttctcca actcgctggc accggccatt gttgccgcgt ccatcaaagt actggagatg
    26641 gtcgaagcgg gcagcgaact gcgtgaccgt ctgtgggcga acgcgcgtca gttccgtgag
    26701 caaatgtcgg cggcgggctt taccctggcg ggagccgatc acgccattat tccggtcatg
    26761 cttggtgatg cggtagtggc gcagaaattt gcccgagagc tgcaaaaaga gggcatttac
    26821 gtcaccggtt tcttctatcc ggtcgttccg aaaggtcagg cgcgtattcg tacccagatg
    26881 tctgcggcgc atacccctga gcaaattacg cgtgcagtag aagcattcac gcgtattggt
    26941 aaacaactgg gcgttatcgc ctgaggatgt gagatgaaag cgttatccaa actgaaagcg
    27001 gaagagggca tctggatgac cgacgtccct gtaccggaac tcgggcataa cgatctgctg
    27061 attaaaatcc gtaaaacagc catctgcggg actgacgttc acatctataa ctgggatgag
    27121 tggtcgcaaa aaaccatccc ggtgccgatg gtcgtaggcc atgaatatgt cggtgaagtg
    27181 gtaggtattg gtcaggaagt gaaaggcttc aaaatcggcg atcgcgtttc tggcgaaggc
    27241 catatcacct gcggtcactg tcgcaactgt cgtggcggtc gtacccatct gtgccgcaac
    27301 acgatcggcg tcggcgttaa ccgtccgggc tgcttcgccg aatatctggt gatcccggcg
    27361 ttcaacgcct tcaaaatccc agacaatatc tccgacgacc tggcttccat ttttgatccc
    27421 ttcggtaacg ccgtgcatac cgcgctgtcg ttcgatctgg ttggcgagga tgtgctggtc
    27481 tctggtgcag gtccgatagg tattatggct gcggcggtgg cgaaacacgt tggtgcacgc
    27541 aatgtggtga tcactgatgt taacgaatac cgccttgagc tggcgcgcaa aatgggtatc
    27601 acccgtgcgg ttaacgtcgc gaaagaaaac cttaatgatg tgatggctga actgggcatg
    27661 accgaaggct ttgatgtcgg tctggaaatg tccggtgcgc cgccagcgtt tcgtaccatg
    27721 cttgacacca tgaaccacgg cggccgtatt gcgatgctgg gtattccgcc gtctgatatg
    27781 tctatcgact ggaccaaagt gatctttaaa ggcttgttca ttaaaggtat ttacggtcgt
    27841 gagatgtttg aaacctggta caagatggcg gcgctgattc agtctggcct cgatctctcg
    27901 ccgatcatta cccatcgttt ctctatcgat gatttccaga agggctttga cgctatgcgt
    27961 tcgggccagt ccgggaaagt aattctgagc tgggattaac acgaacaagg gctggtattc
    28021 cagccctttt atctggggat aatctgttga atatgtaaaa tcctgtcagt gtaataaaga
    28081 gttcgtaatt gcgctgatct cttatatagc tgttctcatt atctctctac cctgaagtga
    28141 ctctctcacc tgtaaaaata atatctcaca ggcttaatag tttcttaata caaagcctgt
    28201 aaaacgtcag gataacttca gaggtcgtcg gtaatttatg atgaacagca ccaataaact
    28261 tagtgttatt attccgttat ataatgcggg cgatgatttc cgcacttgta tggaatcttt
    28321 aattacgcaa acctagactg ctctggaaat cattattatt aacgatggtt caacggataa
    28381 ttctgttgaa atagcaaagc attacgcaga aaactatccg cacgttcgtt tgttgcatca
    28441 ggcgaatgct ggcgcatcgg tagcgcgtaa tcgtgggatc gaggtggcga cgggcaaata
    28501 tgtcgctttt gtcgatgctg acgatgaggt ctatcccacc atgtacgaaa cgctgatgac
    28561 catggcgtta gaggacgacc tcgacgtggc gcagtgcaac gctgactggt cttttcgtga
    28621 aacgggagaa acctggcaat ccatccccag cgatcgcctt cgctcaaccg gcgtattaac
    28681 cggcccggac tggctgcgga tggggctttc ttcgcgccgt tggactcacg ttgtctggat
    28741 gggggtttat cgccgtgatg ttattgttaa aaataacatt aaatttattg ccggattaca
    28801 tcatcaggat attgtctgga caacagaatt catgtttaac gcgctgcgtg cgcgatatac
    28861 cgagcaatca ttatataaat attatctgca taatacgtca gtgagtcggt tgcatagaca
    28921 agggaataaa aaccttaatt atcaacgtca ctatattaag attacccgcc tgctggagaa
    28981 attaaatcga aattatgccg acaaaattac gatttatccg gaatttcatc agcaaataac
    29041 ttacgaagca ttgcgtgttt gccatgcggt gcgcaaagag ccggatattc ttacccgcca
    29101 acggatgatt gccgagatat ttacttccgg tatgtataag cgcctgatta ccaatgtgcg
    29161 cagcgtgaag gtcggttacc aggcgttact gtggtctttc cgcttatggc aatggcgcga
    29221 caaaacgcgg tcgcaccatc gcattacgcg tagcgccttt aatttgcgct agcgttaaac
    29281 ttcgccgctt cgggctgttt gccccagcct tgccactgat gctggaagta aacgatcagc
    29341 gtgctctggc tgatgctttc gcttaatact tcaaagaagc gattagcata aacaggttct
    29401 atcggtttct tcggtttgca cagcttcacg ccacggaacg gattacgcgg tgcatcaggc
    29461 acagcgtttt tcggcggtgt agaagtatca acctgcggtt cattcagcaa gctgctggct
    29521 ttcaccagcg taatgtctgg cggcaggtta taaaccatct gttgtagcac gcgtaccgtt
    29581 aaagggtgag gatgcccaat ggcaatggtt gaaccgttgc gacgcgccag atcaattgcg
    29641 cgattaaatt gcacacggat gtccgcttcg ttctgcgaat cgtcgaggaa caccttccgt
    29701 ttaataacct tcacgccagt accttgcgcg gcgcgcatcg cctgggtatt accgatggtt
    29761 acgctgtcga ggaagtaaag attgtaacgc tccagtgcct gcatcacttt ctgcatacca
    29821 aacaggttag aggtcatctt gctacccatg tggttgttga tccccacggc atagggcacg
    29881 ttattgaccg cactacgaat aatgcgctca atttcgtcgc tgctcatctc cgggcgcagc
    29941 gtatttttct ccagcggctg tttactcagt ggtgccatcg ggagatgaat caacacttcg
    30001 tgcccgctgt tatgcgcttt ggtcgccatc tctctggcgt gcggtgaatc gggtaataca
    30061 gcgacggaga tagcggaggg catcgccagc acctggtttt cgttgtgcgg gcgataccca
    30121 aaatcatcaa tgacgatggc aagtttgcca gcaagtacgg gggaggagag cgccaacaga
    30181 gcggcaaatg caagaacgtt acgacgaaat ggaaacaaaa cttatcttcc caaccacggc
    30241 tgtggattga ccgcctgacc ctggcggcga atttcgaaat agagtgaagg ccgaccctga
    30301 ccgccactgc tgcccaccag tgcaattggc tggcccgcgc gaacctgcga accaacgctc
    30361 accagtgcgc tctgattata gccgtaaaga ctcatgtcgc ctttaccgtg ctcaaccacc
    30421 accaccagac cgtaaccttg cagccagtca gccagaatca ctcgaccgtc ggcaattgct
    30481 ttaacttcag taccttcaga ggcaccgata accatccctt tccagcgtaa ttcaccctgt
    30541 agctgctcgc catagcgatg cagcgtcggc ccgcgaaccg gccagaatgc ctgaccacgc
    30601 ggcgcaccca gaccaccagt tcgggacatc agcgattttt cgctttcggt cggtttgtag
    30661 gtggtgcctt tgcgcgtcgc ttctttctgg cggtcgcgaa ccgcctgggc ctcacgcgct
    30721 tctcgttcag cacgcgcttt cgccgcggct tccgcacggg caatgctgtt acgcagacgg
    30781 gattcgttgg cacgcagttc acttagctgc tgctgacctt gctggatgga agattccagc
    30841 cccgccagcg tctttttacg ctcgttcaga gcctgagtca gtttcgcctg ttgggcgcgc
    30901 tgttcatata acagcgtttg ttgctcgctt tgtttctctt ccagttcagc acgctgcatg
    30961 gcgacttctt cacgcgtttg cttcaactgg gcaatggttt cttgtcgcgc ctggttgaga
    31021 tagccgaaat aagcctgtaa acgctgtcca cactggcttt cttcaccgct gagaattagc
    31081 tgaataccgg tatgctcgcc ctgacggaat gcggcatcca gttgtgcggc gaggctgcgc
    31141 tcctgggcgg ctttttgctg ctccagtttg gcaatcgacg cgttcatctc atcaatctgt
    31201 ttattgagtt gattgagcgt gttttgcgtt tcgcgcagct tacgggtggc ttcagagatc
    31261 gcttcttcct gctttttcaa ttgtgcgagc aggcttgcgc gttgttgttg cttttggcgt
    31321 accgcgcgct cttttgcggc gatatcggcc tgaatagatt tgagttggtc acgctcatcc
    31381 gcgtgggcgg aaaaggcgca caacaatacg ccagcgctaa gaacgctggc gtagatgatg
    31441 ggcctgattg caaacctgcg cggtttcacg gcccgtgtca tggtattaat cgcctttccc
    31501 ctcatgggga gggattattc cacgatgaac agcggcttac cagtcatctc ttgcgggatt
    31561 tccataccca tcagcgacaa catggtcggc gcgatgtcag aaagtttgcc gcctgcaacc
    31621 gctttcacgt tcttatcacc aacgtaaatc agcggaactg gcaggttggt gtgtgccgtg
    31681 tgtgcctgac ccgttgccgg attgcgcatc tgttcagcgt taccgtggtc agcggtgatc
    31741 agcagttgtc cacccacgga ctcaaccgct ttcgcgactt cttccacgca gtgatccagt
    31801 gcttcaaccg ctttaaccgc cgcttccatc accccggtgt gacctaccat gtcgccgttc
    31861 ggatagttac agataatggt gtcgtatttg ccgctcttga tggccgcaac cagtttttcg
    31921 gtcagctctg cggagctcat ttccggttgc agatcgtagg tagccacttt cggtgagttg
    31981 atcagaatgc gatcttcgcc tttgaatgac tcttctacgc caccgttgaa gaagaaagta
    32041 acgtgggcat atttttcggt ttcggaaata cgcaactgag ttttgtcgtt tttcgccatc
    32101 cactcgccga aggtgttaac cagggaagcg ggtgggtaag caaccgcagt tttgatgtca
    32161 gcggcgtatt cggtcagcat cacgaaatcg acgttaacca ctttcttacg cgcgaagccg
    32221 tcgaaatctg cgtttacgaa tgcacgagtg atttcgcgcg cgcggtcagc acggaagttc
    32281 atgaaaatca gcgcatcgcc gtcttccatt gccgcatctg gctgaccttc tgcacgaata
    32341 acggtcgctt tcacgaactc gtcgttttcg tcgcgagcat aagctgcctg caaaccagca
    32401 acggcagtat cggcctggaa ctcaccctgc gccagagtca gcaggtcata ggctttttca
    32461 acgcgatccc aacggttatc gcggtccatc gcgtagtaac gaccaatgat ggacgctacg
    32521 cggcctttac ccagcgcagc aaatttctct tcaaattttt tcagcgagga ttcagcactg
    32581 cgcggcgggg tgtcgcgacc gtcaaggaat gcgtgcaggt agattttttc tgcgccgcgt
    32641 tcagctgcca gttctaccat cgccatgatg tgatcttcgt ggctgtgtac gccgcctgcg
    32701 gagagcagac ccataatgtg tactgcttta cctgcgtttt tcgctttatc taccgcgcca
    32761 gtcagcactg gattagcaaa gaaagcgcga tctttgattt caacgtccag acgagtcagg
    32821 tcctgataca cgatgcggcc ggcacccagg ttaacgtggc ctacttcgga gttacccatc
    32881 tgacggtcag gcagaccgac ttccagaccg gaagcgtcga tcagggtatg cggacgattg
    32941 gcccacagtg catccattac cggggtttta gcactaaaaa tggcgttatc ctgctgttct
    33001 tcacgatagc catagccatc cagaatcacc agtaccatag gttttttaga aaccgacatt
    33061 gcgacaacct catactcaag agtcaaaatt tgcgtaattt tactacagct gaatcgataa
    33121 aatagcctct gaagatcaaa atatacgacc gctcgcggca agccgatgcc agatttgcgc
    33181 ttttttttcg tcgtgccccg caaaattgca ttccagttaa cgcgctggct gtatttgccg
    33241 caccgcgcag gtatactcct ttcctggttt ttttaatcac tacgtcggga gttgttaccc
    33301 cccatgcaag aaattatgca atttgttggc cgtcatccca tactgagtat cgcttggatc
    33361 gcgttactgg tggcggttct tgtgactacg tttaagagcc tgacctcgaa agtgaaggtg
    33421 attactcgtg gtgaagctac gcgtctgatc aacaaagaag acgctgtggt tgtggattta
    33481 cgtcagcgtg atgacttccg taaaggccat atcgcaggtt ctatcaatct gttgccgagc
    33541 gaaatcaaag ccaacaatgt tggtgagctt gagaagcaca aagacaaacc ggttatcgtg
    33601 gtagacggtt ctggcatgca gtgccaggag cctgcaaacg cgctgacgaa agctggtttt
    33661 gcgcaagtat tcgtgctgaa agagggcgtt gctggctggg ctggcgaaaa cttgcctctg
    33721 gtgcgcggca aataatttac ctgtcgtctt tcatgatgca ggccgttagc tgcaattagg
    33781 cccccagcga cttattgtta taagcttctg gggacactcc attttgcagc cgccctgcaa
    33841 catgaaattt cagggtaaaa aaacaggagc taaattatgg ccaatgttga aatctatacc
    33901 aaagaaacct gcccgtattg ccatcgtgca aaagcactgc tgagcagcaa gggcgtgagt
    33961 tttcaggagt tgccgatcga tggcaatgcc gccaagcgtg aagagatgat caaacgcagc
    34021 ggtcgcacca cggttcccca gatttttatt gacgcacagc acattggcgg ctgtgatgac
    34081 ttgtatgcat tggatgcacg tggtggactg gatcccctgc tgaaataacg tgtgaacgtt
    34141 ggcattacat tgcgcagtat ttaaggacaa cacttaaggg ttttctacac atgtcagaac
    34201 aaaacaacac tgaaatgact ttccagatcc aacgtattta taccaaggat atctctttcg
    34261 aagcgccgaa cgcgccgcac gttttccaga aagattggca accagaagtt aaacttgatc
    34321 tggatacggc atcttcccaa ctggcagatg acgtatacga agtggtactg cgtgttaccg
    34381 taacggcctc cctgggcgaa gaaaccgcgt tcctgtgtga agttcagcag ggcggtattt
    34441 tctccatcgc gggtatcgaa ggcacccaga tggcacattg cctgggcgca tactgcccga
    34501 acattctgtt cccgtatgct cgtgagtgca tcaccagcat ggtatcccgc ggtacattcc
    34561 cgcaactgaa ccttgcgccg gttaacttcg atgcgctgtt catgaactat ttgcagcagc
    34621 aggctggcga aggtactgaa gaacatcagg atgcctgatg aaccaacgta atgcttcaat
    34681 gactgtgatc ggtgccggct cgtacggcac cgctcttgcc attaccctgg caagaaatgg
    34741 ccacgaggtt gtcctctggg gccatgaccc tgaacatatc gcaacgcttg aacgcgaccg
    34801 ctgtaacgcc gcgtttctcc ccgatgtgcc ttttcccgat acgctccatc ttgaaagcga
    34861 tctcgccact gcgctggcag ccagccgtaa tattctcgtc gtcgtaccca gccatgtctt
    34921 tggtgaagtg ctgcgccaga ttaaaccgct gatgcgtcct gatgcgcgtc tggtgtgggc
    34981 gaccaaaggg ctggaagcgg aaaccggacg tctgttacag gacgtggcgc gtgaggcctt
    35041 aggcgatcaa attccgctgg cggttatctc tggcccaacg tttgcgaaag aactggcggc
    35101 aggtttaccg acggctattt cgttggcctc taccgaccag acctttgccg atgatctcca
    35161 acaattgctg cactgtggca aaagtttccg cgtttacagc aacccggatt tcatcggtgt
    35221 gcagcttggc ggtgcggtga aaaacgtcat tgccattggc gcggggatgt ccgacggtat
    35281 cggttttggt gcgaatgcgc gtacggcact gatcacccgt gggctggctg aaatgtcgcg
    35341 tcttggcgcg gcgctgggtg ccgagcctgc cacctttatg ggcatggcgg ggctgggcga
    35401 tcttgtgctt acctgtaccg acaaccagtc gcgtaaccgc cgttttggca tgatgctcgg
    35461 tcagggcatg gatgtacaaa gcgcgcagga gaagattggt caggtggtgg aaggctaccg
    35521 caatacgaaa gaagtccgcg aactggcgca tcgcttcggc gtagaaatgc caataaccga
    35581 ggaaatttat caagtattat attgcggaaa aaacgcgcgc gaggcagcat tgacgttatt
    35641 aggtcgtgca cgcaaggacg agcgcagcag ccactaaccc cagggaacct ttgttaccgc
    35701 tatgacccgg cccgcgcaga acgggccggt cattatctca tcgtgtggag taagtaatgt
    35761 cgtgtgaaga actggaaatt gtctggaaca atattaaagc cgaagccaga acgctggcgg
    35821 actgtgagcc aatgctggcc agtttttacc acgcgacgct actcaagcac gaaaaccttg
    35881 gtagtgcact gagctacatg ctggcgaaca agctgtcatc gccaattatg cctgctattg
    35941 ctatccgtga agtggtggaa gaagcctacg ccgctgaccc ggaaatgatc gcctctgcgg
    36001 cctgtgatat tcaggcggtg cgtacccgcg acccggcagt cgataaatac tcaaccccgt
    36061 tgttatacct gaagggtttt catgccttgc aggcctatcg catcggtcac tggttgtgga
    36121 atcaggggcg tcgcgcattg gcaatctttc tgcaaaacca ggtttctgtg acgttccagg
    36181 tcgatattca cccggcagca aaaattggcc gtggcattat gctcgaccac gccacgggca
    36241 tcgttgtggg tgaaacggcg gtaattgaaa acgacgtatc gattctgcaa tctgtgacgc
    36301 ttggcggtac gggtaaatct ggtggtgacc gtcacccgaa aattcgtgaa ggtgtgatga
    36361 ttggcgcggg cgcgaaaatc ctcggcaata ttgaagttgg tcgcggcgcg aagattggcg
    36421 caggttccgt ggtgctgcaa ccggtgccgc cgcatacaac cgccgctggc gttccggctc
    36481 gtattgtcgg taaaccagac agcgataagc catcaatgga tatggaccag catttcaacg
    36541 gtattaacca tacatttgag tatggggatg ggatctaatg tcctgtgatc gtgccggatg
    36601 cgatgtaatc atctatccgg catacagtaa ctaatctctc aacaacgctc ccggatatcc
    36661 cagctgccgc caggcttcat acaccactac cgacaccgca ttggacagat tcatgctgcg
    36721 gctgtccggc accatcggaa tgcgaatttt ttgttcagcg ggcagggcat caagaatgct
    36781 cgctggcagg ccgcgtgttt ccgggccgaa catcagataa tcgccatcct gatagcttac
    36841 ggcgctgtga gcaggcgtac ctttcgtggt gagggcaaac aggcgctggg gattttctgc
    36901 ttcgaggaac gcgcgatagt catgatgacg cgtaacggcg gtaaactcgt gatagtccag
    36961 ccccgcgcgg cgcaggcgct tatcgtccca ggcaaatccc atcggttcga tgatatgcag
    37021 acgaaagccg gtattagcgc aaagacggat gatgttgcca gtatttggcg gaatttctgg
    37081 ttcgtaaagt acgatgttta gcatactgcc cccttagtgc gggggcagga tagcagaaaa
    37141 ttggtgaacc gtgcggcctt ttcccctcac cccggccctc cccctgtctc ttgatcagga
    37201 gatctgatca agagacagct ccctgaggga gagggttagg ggggtgcccc tattaagttg
    37261 tcaagcatgt tatgacccct gcggggtata aaaagtcccg tcgcgcatca tggcgaacag
    37321 aa
//
LOCUS       DAJVTY010000017        36987 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVTY010000017 DAJVTY010000000
VERSION     DAJVTY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36987
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.017"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..171
                     /locus_tag="OQQ66_000823"
     CDS             <1..171
                     /locus_tag="OQQ66_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1903842.1"
                     /translation="GIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYISYNKSSLNNLV
                     FSRFIDRLNESF"
     gene            318..1835
                     /locus_tag="OQQ66_000824"
     CDS             318..1835
                     /locus_tag="OQQ66_000824"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCS1903843.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1836..2985)
                     /gene="ybdL"
                     /locus_tag="OQQ66_000825"
                     /pseudo
     CDS             complement(1836..2985)
                     /gene="ybdL"
                     /locus_tag="OQQ66_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3094..4182
                     /gene="hcxA"
                     /locus_tag="OQQ66_000826"
     CDS             3094..4182
                     /gene="hcxA"
                     /locus_tag="OQQ66_000826"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1903844.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4192..4389)
                     /locus_tag="OQQ66_000827"
     CDS             complement(4192..4389)
                     /locus_tag="OQQ66_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1903845.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4571..6676)
                     /gene="cstA"
                     /locus_tag="OQQ66_000828"
     CDS             complement(4571..6676)
                     /gene="cstA"
                     /locus_tag="OQQ66_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1903846.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6857..7270)
                     /gene="entH"
                     /locus_tag="OQQ66_000829"
     CDS             complement(6857..7270)
                     /gene="entH"
                     /locus_tag="OQQ66_000829"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1903847.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7273..8019)
                     /gene="entA"
                     /locus_tag="OQQ66_000830"
     CDS             complement(7273..8019)
                     /gene="entA"
                     /locus_tag="OQQ66_000830"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1903848.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8019..8876)
                     /gene="entB"
                     /locus_tag="OQQ66_000831"
     CDS             complement(8019..8876)
                     /gene="entB"
                     /locus_tag="OQQ66_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1903849.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8890..10500)
                     /gene="entE"
                     /locus_tag="OQQ66_000832"
     CDS             complement(8890..10500)
                     /gene="entE"
                     /locus_tag="OQQ66_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1903850.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10510..11685)
                     /gene="entC"
                     /locus_tag="OQQ66_000833"
     CDS             complement(10510..11685)
                     /gene="entC"
                     /locus_tag="OQQ66_000833"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS1903851.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12060..13016
                     /gene="fepB"
                     /locus_tag="OQQ66_000834"
     CDS             12060..13016
                     /gene="fepB"
                     /locus_tag="OQQ66_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1903852.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13020..14270)
                     /gene="entS"
                     /locus_tag="OQQ66_000835"
     CDS             complement(13020..14270)
                     /gene="entS"
                     /locus_tag="OQQ66_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1903853.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14381..15385
                     /gene="fepD"
                     /locus_tag="OQQ66_000836"
     CDS             14381..15385
                     /gene="fepD"
                     /locus_tag="OQQ66_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1903854.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15382..16374
                     /gene="fepG"
                     /locus_tag="OQQ66_000837"
     CDS             15382..16374
                     /gene="fepG"
                     /locus_tag="OQQ66_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1903855.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16371..17186
                     /gene="fepC"
                     /locus_tag="OQQ66_000838"
     CDS             16371..17186
                     /gene="fepC"
                     /locus_tag="OQQ66_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1903856.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17183..18316)
                     /gene="fepE"
                     /locus_tag="OQQ66_000839"
     CDS             complement(17183..18316)
                     /gene="fepE"
                     /locus_tag="OQQ66_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1903857.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18531..22412)
                     /gene="entF"
                     /locus_tag="OQQ66_000840"
     CDS             complement(18531..22412)
                     /gene="entF"
                     /locus_tag="OQQ66_000840"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS1903858.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22409..22627)
                     /gene="ybdZ"
                     /locus_tag="OQQ66_000841"
     CDS             complement(22409..22627)
                     /gene="ybdZ"
                     /locus_tag="OQQ66_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1903859.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22630..23832)
                     /gene="fes"
                     /locus_tag="OQQ66_000842"
     CDS             complement(22630..23832)
                     /gene="fes"
                     /locus_tag="OQQ66_000842"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1903860.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24075..26315
                     /gene="fepA"
                     /locus_tag="OQQ66_000843"
     CDS             24075..26315
                     /gene="fepA"
                     /locus_tag="OQQ66_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1903861.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATRDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26490..27110
                     /gene="entD"
                     /locus_tag="OQQ66_000844"
     CDS             26490..27110
                     /gene="entD"
                     /locus_tag="OQQ66_000844"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1903862.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27232..27384)
                     /gene="hokE"
                     /locus_tag="OQQ66_000845"
     CDS             complement(27232..27384)
                     /gene="hokE"
                     /locus_tag="OQQ66_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1903863.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27837..28955
                     /locus_tag="OQQ66_000846"
     CDS             27837..28955
                     /locus_tag="OQQ66_000846"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1903864.1"
                     /translation="MPLPDFHVSEPFTLGIELEMLVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29021..29269
                     /locus_tag="OQQ66_000847"
     CDS             29021..29269
                     /locus_tag="OQQ66_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1903865.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29334..29702
                     /locus_tag="OQQ66_000848"
     CDS             29334..29702
                     /locus_tag="OQQ66_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1903866.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29796..30448
                     /gene="nfsB"
                     /locus_tag="OQQ66_000849"
                     /pseudo
     CDS             29796..30448
                     /gene="nfsB"
                     /locus_tag="OQQ66_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
     gene            30556..31803
                     /gene="ybdG"
                     /locus_tag="OQQ66_000850"
     CDS             30556..31803
                     /gene="ybdG"
                     /locus_tag="OQQ66_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1903867.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGT
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(31871..33247)
                     /gene="pheP"
                     /locus_tag="OQQ66_000851"
     CDS             complement(31871..33247)
                     /gene="pheP"
                     /locus_tag="OQQ66_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1903868.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33349..36492)
                     /gene="cusA"
                     /locus_tag="OQQ66_000852"
     CDS             complement(33349..36492)
                     /gene="cusA"
                     /locus_tag="OQQ66_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1903869.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(36504..>36987)
                     /gene="cusB"
                     /locus_tag="OQQ66_000853"
     CDS             complement(36504..>36987)
                     /gene="cusB"
                     /locus_tag="OQQ66_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS1903870.1"
                     /translation="AAIPESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAAT
                     RTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQGLIDTGSEQRVITIDADGR
                     FVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANISGALERMRSESATHAH
                     "
BASE COUNT         8862 a        10333 c         9441 g         8351 t
ORIGIN      
        1 ggaatagtcc cctatgaact ttataactcc caccgtgact ttcttaattt aaaagagata
       61 aaactagaac atccgttacc ttcaataaag ctttacattt cctataataa atcttcttta
      121 aataatttgg tgttctcccg atttattgac cgtctaaatg agagtttttg atatataact
      181 acagacagct actataactt tcatctattt attcacagcg cgaatgaaaa ataaaacact
      241 ctgctttaca gtgtttactt tataattaac agtcttatct cactgttcac cctgtaacgt
      301 tcagagagcg tcttcagatg tctatttata aaattcctct tccgctcaat attctggaag
      361 ctgcacgaga gcgtattacc tggacactca acaccctacc ccgcgtttgt gtctcatttt
      421 ctggcggcaa agactctggt ttaatgctgc atctgacagc cgaacttgcc cgacaaatgg
      481 gcaaaaaaat ctgcgttttg tttatcgact gggaggcgca attctcttgc actattaact
      541 atgttcagtc cctgcgcgag ttgtacaccg atgtcatcga agagttttac tgggttgcgc
      601 tcccgcttac gacgcaaaat tccctttcac aataccaacc cgaatggcag tgctgggaac
      661 ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct gactttttct
      721 gcttttacca gccaggcatg accttcgaac aatttgtacg tgagtttgcc gaatggtttt
      781 cacaaaaacg tccggcggcg atgatgatcg gcatccgtgc ggatgagtcc tacaaccgtt
      841 ttgtcgccat cgccagttta aataaacaac gttttgccga cgataaacca tggactacgg
      901 ccgcaccagg cggtcatagc tggtacattt accccattta cgactggaaa gtggctgata
      961 tctggacctg gtatgcaaat catcaaagtc tctgcaaccc actgtataac ctaatgtatc
     1021 aggcaggcgt tcctctgcgt catatgcgaa tttgcgaacc ttttggcccg gagcaacgac
     1081 aaggattatg gctctatcac gttatcgaac cggatcgctg ggctgctatg tgcgcacgag
     1141 tcagcggggt aaaaagtggc ggcatttacg ccggacatga caatcatttc tatgggcatc
     1201 ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg ctgctcaata
     1261 gcatgccgga agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg atccatcagg
     1321 ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac agccaactta
     1381 tgcctaatga ttacaaccca aacaacgtgg caccacccga aaaaaaactg ctgcaaaaat
     1441 ccattgaaat agatggtttt acccaaccca ttgtggtcac acatacggat aaaaatgcca
     1501 tggaaatcgt ggatggtttt caccgccatg aaattggtaa aggctcatca tcattaaaat
     1561 tacgcttaaa aggttatttg ccggtgacct gtctggaagg tacgcgtaat caacgcatcg
     1621 ccgcgactat tcgtcataat cgcgctcgtg gacgccatca aatcaccgcc atgtcggaaa
     1681 tcgtccgtga acttagccag ttgggatggg acgataataa aattggcaaa gagctgggaa
     1741 tggacagcga cgaagtgctg cgcctgaagc aaattaacgg cctgcaagag ttgtttgccg
     1801 accgtcaata ttcccgcgcc tggacggtta aatagctaaa gctggcgcag gcgttcagct
     1861 gctgccagca acgtcgattc cttcttggca aaacagagac gaatcagttt atgtgggaag
     1921 ggatcggcgc aaaacaccga cagcggaatc gccgccacgc cgtgctcccg cgtcagccac
     1981 tggcaaaact caacatcatc cagggtagaa acctcgctgt aatccaccag caaaaagtat
     2041 gtaccttcac acggtaaaat ctccagccgg ctttcattta aagcattcac cagaatatcg
     2101 cgcttctggc gataaaagtc cggtaacgca agataatgct caggttctgc acgtagcata
     2161 tcagcaagcg ccagctgtgc cggggtattc accgaaaagg tcagatactg atgtaccttg
     2221 cgaatttcgg cgctgatggg cgctggcgca acacaataac ccactttcca gccggtcata
     2281 tgatagatct tgccaaatga agaaaccgcc actgcccgct cacgcagctg cggatgcgcc
     2341 agcacactgg catggccctg ttgtgaaaag ttgatgtgct cgtagacttc atcgctaatg
     2401 acaaaaatct cgtgcccggc gatcgcctgc cacaaagcgg cgaaatcagc ctgctgccag
     2461 acagttgcac tggggttatg cggagtgttg aggatcacca gtctggtgcg ctcgcttaat
     2521 aatgcggcaa attcctgcca gtcaaggtgg ttgcagtgcc atacgcttca ctattccccc
     2581 agaaagcgcg atggcggggg cgtaactgtc atagctggga tcaaaacaaa tcacttcatc
     2641 gccattgcgc accagtgcgg taatcgccgc gtataacgct tccgtcgccc ctgccgttac
     2701 ggtgatatcg ctatcggcat ctggttgata gccatacaaa cgttccgttt tctgagcaat
     2761 cgcctcgcgc aaggcctgca cgccggtcat gggcgcgtat tggtttgccc cctgggcaac
     2821 gtggtacgcc agccgctcct gtaaatagcg cggaccatca aaatcaggaa agccttgcga
     2881 caggttaatc gcctggtgtt gctgcgccag cgcgctcatc tgggtgaaaa tagtggtgcc
     2941 aagttgtgga agtttgcttt gtggaatcag agggttattt gtcattttta ttgtacctga
     3001 cgaagttgcg tgttgaaggc actataacac gatgctagta tttggcaatc aagacgttta
     3061 gatgtctaaa taaaacaata aggacaacac aacatgcctc acaatcctat ccgcgtggtc
     3121 gtcggcccgg ctaactactt ttcacatcca agaagtttca atcacctgaa cgattttttc
     3181 actgatgaac aactttctcg cgcggtgtgg atctacggca aacgcgccat tgctgcggcg
     3241 caaaccaaac ttccgccagc gtttggactt ccaggggcaa agcatatttt gtttcgtggt
     3301 cattgcagcg aaagcgatgt acagcaactg gcggctgagt ccggtgacga tcgcagcgtg
     3361 gtgattggcg tcggcggcgg cgcactgctc gacaccgcga aagccctcgc ccgccgtctc
     3421 ggtctgccat ttgttgccgt tccgacgatc gccgctacct gcgccgcctg gacaccgctc
     3481 tccgtctggt ataacgatgc cggacaggcg ctgcattatg agattttcga cgacgccaat
     3541 tttatggtgc tggtggaacc ggagattatc ctcaatgcac cgcaacaata tctgctggcg
     3601 gggatcggtg acacgctggc gaaatggtat gaagcggtgg tgctggctcc gcaaccagaa
     3661 acgttgccgc taaccgtgcg gctggggatc aataatgcgc aggccattcg cgacgtcttg
     3721 ttaaacagta gcgaacaggc acttgccgat cagcaaaatc agcagttaac gcaatcattt
     3781 tgcgatgtgg tggatgctat tattgctggt ggtgggatgg tcggtggtct gggcgatcgt
     3841 tttacgcgtg tggcggcggc tcatgccgtg cataacggtc tgactgtcct gccgcaaacc
     3901 gagaagtttc ttcacggcac caaagtcgct tacggaattc tggtgcaaag cgccttgctg
     3961 ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc agcgttttca tctgccgact
     4021 acgctggcgg agctggaagt ggatatcaat aatcaggcgg agatcgacaa agtgattgcc
     4081 cacaccttgc gcccggtgga gtccattcat tacctgccag tcacgctgac accagatacg
     4141 ttgcgtgcag cgttcgaaaa agtggaatcg tttaaagcct gacgtgcaaa tttagcagca
     4201 gcgcgcgccg ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa actcctcgta
     4261 ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt tcgacatagt tgtcgtaatc
     4321 aggcatacca atcatcagct tcgccgcctg acctaaatat tttccggctt ttgccagtga
     4381 atcaaacatc ttggcctccg ctaaccatgt tgctgaaaga gtgccggatg cggcgtgaac
     4441 gctttatccg gcctacaaat gctatcaacg cactcggtca gtcccctcgc cccatcgggg
     4501 agagggttag ggtgaggggg aaccctcggc actatgccaa cattcgccaa catcccccct
     4561 cactctgact ttagtgcgcg cctttcgcct gcgctacgat ctcctcgaca ttttccggca
     4621 tcggctcata cggcgtttct ttcgccgttg gcttcggatc tttcaatgcc gcaagtgccg
     4681 tcttaatcga gaacagtgcc agaaccacca cgaccaccat aaagaagatg gttaacccgg
     4741 catccagacg gttgttgaac accagttgcg ccagctgtga ctcagtatac tgcgacggaa
     4801 tattgccgct gtcgatcatt gcctggaact tgttagcaat ggccaggaag ccgactttcg
     4861 catccgggct aaacgctttc tgccagccag cggtcagggt acaaatcagc agccaggccg
     4921 ttggtaccag cgccacccag gcgtaacgtt gacgtttcat cttgaacaac accacggcac
     4981 agagcatcag cgccatccct gccagcatct ggttggcaat accaaacagc ggccacagag
     5041 tgttaatacc gcccagcgga tcgaccacgc cctgatggag gaagtagccc cacgccagta
     5101 cgcacagcgc cgttgccagc aggttagcag gcagtgaatc ggtccgcttc aggccaggag
     5161 acaccacgcc cagcagatcc tgcaacataa agcgcgcagc acgcgtaccc gcatctaccg
     5221 ccgtcagaat aaacagagct tcaaacagaa tggcgaagtg ataccagaac gccacatcca
     5281 tcataccacc cagcgcaccg tgcaggatgt aagccatccc caccgccaac gtcggcgcac
     5341 cgcccgcacg ggaaatgatc gactgttcac ccacttcgct ggcaatctgg ttgagcgtat
     5401 ctggtgtaat gctaaagccc cagctactca ccacctgcgc ggcagaagcg accacatccg
     5461 ccgtccctgc tggagccagc accgccattg ggctgttcat ggcaaagtaa acgcccggat
     5521 cgatgataca ggcggaaacc agcgccataa tcgccacgaa ggattccatt aacattccac
     5581 cgtagccgat aaaacaagcc tgcccttcgt tcgccagcat cttcggcgtg gtgccggaag
     5641 agatcagcgc atggaagcca gacaccgcgc cacaggcgat ggtgataaac aggaacggga
     5701 acaggttacc ggtccatacc gggccagtgc catcgacaaa tttggtcagc gcaggcatgg
     5761 tcagcgtcgg gcgcataatc aaaatgccta ccgccagacc gacgatcgtc ccgattttca
     5821 ggaaggtaga gaggtagtca cgcggggcca gcagcaacca caccggcagc accgccgcca
     5881 caaaaccgta acccaccagc atccaggtca gctgcacgcc ggtaaagtca aagtacggtg
     5941 cccaggtcgg actttctgcc acccagccgc cagagataat ggcgaaaatc aggaatacca
     6001 gaccgatgac cgacacttca ccaatacgcc ccggacgcag atagcgcagg tagatcccca
     6061 taaacagcgc cagcggaatg gtgaacgcaa cggtatacgt tccccacggg ctatgagtca
     6121 gggctttcac cacgatcatc gccagtactg caaggataat gaccatgatc ataaagcagg
     6181 ccaccagcgc tatcaccccg gcggttggcc ccatctcttc tttgaccagc tcacccagcg
     6241 atcgaccgtc acggcgcgta gaaacaaaca gcaccatgaa atcctgcacc gcaccggcga
     6301 gaaccacccc ggcgagtagc cagatcatcc ccggcaggta gcccatttgc gctgccagca
     6361 ccggcccaac caacggacct gctccggcga tggccgcaaa atggtgaccg aacagcactt
     6421 tcttgtccgt cggcacatag tccagcccgt cgttatggcg caccgctggc gtcatacgct
     6481 tcgggtcaac cgccagcaca tttttggcga tatacaaccc ataaaaacgg taagcgatca
     6541 gatagataca gaccgacgcc accacaatcc acagcgcgtt gatctgttcc ccacgattta
     6601 aagcaatgta tcccagagca aatgctccca ttacagagag cactgtccag acgaggtatt
     6661 tccctgattt gttcatagtt gttatccgtg tgcgtgtcca tagagatgtt acattttgtt
     6721 tctataacat ctttgtacga tttaacaaca cggtaactat gtaaatcgga gttgaaacca
     6781 atatttaact taacaatgtt aactcgatca ctccgttaac cgagttttcc tgcaatctca
     6841 tttaattctg tcgggctcat cccaaaactg ccgtacccag ccgacaagtg cagcaacgcc
     6901 gcccctgttc atcgaaaacg acgatttccc agctctgatt ttgccgccca agatgcagcg
     6961 gctggcagac gccgcgtacc ttgccttcag acaccgggcg atgatgggtg gcgttaagtt
     7021 ccgtgcccac cacacactgc ccgtcacggg tcatcataaa tccggccatc gatcccagcg
     7081 tttcagccag cgccgccgac gcgccgccat gcagcaggcc aaacggctga tgagtacggg
     7141 tatcaaccgg catttcggct tccagcacat catctcccag acgggtatac acaattccca
     7201 gatgcgccac cattgtgtta tcgctggtgg cgttcagttc gtcgagcgtt aaatggcgtt
     7261 tccagatcat gcttatgccc ccagcgttga gccgccatcg accacaatat cctgtagggt
     7321 gatatggctg gcgaggtcag aggcgaggaa caaaatcgtg ttggcgatct cttgtggacg
     7381 ggcgattttc cccagcggaa tgccgagttt aaactgctcg ccaaagccgc gaatacgctg
     7441 ttcttcggcg tcatcgctca cccacagcgt gcgttgcata tcggtgtcgg tggagccagg
     7501 cgaaaccaca ttacagcgca cgccgctacc cgccagttcc agcccgacgc tcaacgccag
     7561 gcttttcagc gccgctttcg atgcgccata agcactcata ccaatacgcg gcgtgtgtgc
     7621 ggcgtcggac gccacagtga caatcgcccc gccccgctga cggcgaaact ggttcatggt
     7681 ttgctggaac aggttaaacg caccgccgac gttaaccgca aaagtctgct gccagtcctc
     7741 tttactgagc tgatcggtcg cgcccatgcg caaaattccc gccgcattga tcagcacgtc
     7801 cagtcgctcc gtttcagcta acagtcgctg acatacctgc gccacctgcc cggcgtcggc
     7861 aacatccatt acttcggtcg caaagggata ttgctcctga gtgaacgctt gatcaaaacc
     7921 tgtaactttc gctcccgcct caacaaacgc cagcgccgtg gcgtagccga tacctttacc
     7981 tgcgccggtt acccagacat ttttaccgct gaaatccatt atttaacctc gcgggagagt
     8041 agcttccacc aggcgtcgat ggtcgggttt ttcgccagca tgacaaagtc gatatcacca
     8101 tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca ccgaatccag accgtagtcg
     8161 atcaggttgt cgtcatcgaa cggttcatcg gactcgtcca gcaacggcag gataacctca
     8221 cgcagcgccg ctttgctggc ggggacaggt gctggcagta attcttcagt catcaccacc
     8281 cggccagacc gcccggccac atatttcagc gacatcaaat gctcgtcacg gctgaaatcg
     8341 gccagcgcat ccgccaccat aaacggttta atatcgcgca taaatgcgtc ggttgcggtg
     8401 gtcatacagc caatgtgggc atataccccg gtaataatca gctggttacg tccactctct
     8461 ttgagcattt gctccagcgg agaacgatga aacgcgctgt agcgccactt caccagcacc
     8521 gtgtcgtcgg catctggcgt caggcgatcc accacctttt gctgttccgg cgaacgggtc
     8581 aggcccggcc cccacatatc attcaacagc gcccgatctt catcgctctg ctctttcggc
     8641 tgggcggtgt aataaactgg gatattgtgc tgtttgcagt agtcgcgcag cgcagcaata
     8701 ttcgcgatca cctgctccat catcgggcag ttctcgcccc agaagctgac aaaatagtcc
     8761 tgcatatcat ggattaacaa cgcggcacgt tgcagttcaa aggcccagtc aactttattc
     8821 tgcggaatat cgtgagactc cggcagtgcg taagcctgta attttggaat agccatcgtg
     8881 ttctctcctt caggctgatg cgcgtgacgc cagccactga cttaattgtt ttttatcgac
     8941 tttcccgacc gccgtcagcg gaagtgaatc tacacactcc acgcgatccg gtaatttaaa
     9001 ttcggcaata ccctgttcac gcaggaaacg acgcacctgc accgcgcgca gcggctcttt
     9061 taccaccaga taagcacagc ttttttcgcc catcagctca tcttccatgc tcaccagtgc
     9121 ggcatagatc accgccggat ggcgcagcag caggttttcg atctcttcgg cagcgatctt
     9181 ctcgccgcca cggttgatct gatctttctc gcgcccctgc acggtgatgt aaccctctgg
     9241 atcaatagag atcagatcgc cggaacagta aaaaccgttg gcatcaaagg cgctggcatt
     9301 gtgctgcgga cttttgtaat aaccgcggaa ggtgtacggc ccgcgcgtca tcaggcgtcc
     9361 gacttcccct tgcggcagtg gatttccttc ggcatcggca acccacactt catcgtccgg
     9421 acacattggg taaccctggg tatggataat tttctccgcg ctatcatcaa gacgggtgta
     9481 gttcaccagc ccttccgcca tgccaaacac ctgctgcaac tggcagccaa tctcagcggg
     9541 aatacgcgcc gcaagggtgg cagaaagacg tgcgccgccg acctgtaaca gtttcagcga
     9601 ggcaagctgc gcccggcttt cgccttcagc cagcgcctgc aaccacaggc tgactgccgg
     9661 cggcaccagc gcggtgacgt tcacctgatg tttttcaatc aatgggaagc aaagcgtagc
     9721 gctgggatcg gccgccagaa caaccgttcc tccggcaaga aagacgccca gcgatcctgg
     9781 cgaactcatg gcgtagttat gagccgccgg gatcgcgcac aggtagcgcg tctgttgtgt
     9841 gaactgacaa atctcgacgc tacgacgcac gctgtagtag tagtcgttat gagtgcgcgg
     9901 gatcagcttc ggtgtaccgg tggtgccgcc ggaaagctgg aaataggcca cttcatcagc
     9961 aggtgatggc gtggcagtaa aaccgtctgc cggatggtta atcgcatcct gcaagttatg
    10021 ctcaccgctg tcgttgtgca gttgcaccac gcgaatggag gaatgttctg tgacgaaagt
    10081 attgaggaaa tcatccccgc taaacagcgc atgttggcga tcggcaatca gcaatgcggg
    10141 ttcaatctgg ctggcatagg cgttcagttc actacgctga tggctgaaca acgccagcac
    10201 cggcgcaacg cccagtttca gcagcgcgaa aaaggtaata tacaattcag cgacgttacc
    10261 cagttgtacc agcgcggttt caccaggttt aatgccctga cggcgtaaag tacacgcgag
    10321 gttatccgcc gcctgattca gctcccggta actcaactgt agctcgccgt cgataaccgc
    10381 gatgctgtca ctcgcagcgt ggcgagtcag aatgtcggtc agcggcaaat cctgccagta
    10441 gcctttttcc cgatagcgac gggcaaactc ttccggccag cgggtgaatg gaatgctcat
    10501 cctcgctcct taatgcaatc caaaaacatt caacatggta gaaagtttga cgcctgtttc
    10561 gcgccactca cccaacggtg acgacgcaag cacaatcccc gctccggcaa acagacgcac
    10621 ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc cattcgccgt taccttcgct
    10681 gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg aacggctcca gttcagcaat
    10741 aacctgagtc gcggcctgat gcgggaaacc gctcagcgcg ggggtcggat gcagcagaca
    10801 ggccagagtc agtgcgtttt cttgcgaatt cgctttacct tcaaagggag ttgcgagatg
    10861 ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga acgtgtaact cactactgcg
    10921 ttcgcgcagt acctctttca tcgcctgagt caccagttca tgttcatggc gatctttttc
    10981 tgacgccagc agacgattac ccgcttcgcg atcgagcact tcatccggct gacgacgcgc
    11041 ggaaccggct aacggaatgg agctaaaacg ctcgccgtct ttacgtagca gcagttccgg
    11101 gctggccccc agcaggacgc caccatcagc cagcgggaca tggaagttgt aactaaccgg
    11161 gttttgcgca atcaaccgtt ccagcaatac gccactatca atggcggcgt cagtggtgat
    11221 atcaatcaac cgtgacaaca ccactttgtc aacctgcggc gtggcggtaa gtgcggcagc
    11281 gcgggcaacc atctgttcaa acgtggtttg ttccggaatt gcctggcgtt ccaccacatt
    11341 cagcgactgg ctgcgggtga aacggcgggc ggaagcttgt ttttcctgac gggagaacga
    11401 ctgccaggat tcaggaatat acagcgacga aggctgacgt ggatcgaagg gaatcgcccc
    11461 gaccatcacc ggatttttga tgccctgcac tttggcatcg gcaaacagcg cggcgagttt
    11521 ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc ggttcatcga agcgggcgaa
    11581 acatcctgac gtcgtaaaac tgcggtacgg cgacataaag aaaaagcgat tgggcgcaag
    11641 tgttgccatg gtctgctgta cttcctcagc cagtgacgta tccatatcat cctccacaaa
    11701 atgataaagg ctttaataat gattatcatt tatattttcg gtcgctaacc taaaagcaaa
    11761 cgcgcactat gtcaactctt gaggtaacgc aatctgcaac tcggggttgc atctggttgt
    11821 ggttacaatg aaaatgagaa gcattattga tggattcgca taagcgcaat gtgatgtcct
    11881 gcgccgttct tccccctctc ccttccaggg agagggctgg ggtgagggtc aatattcgca
    11941 ccagtgctgg ctgctcccat caccctaacc ctctccccaa aggggcgagg gtacggatag
    12001 tgcgctttgt cgaatttgtc attacgccct taaccttatt aataacagga agctgatttg
    12061 tgagactcgc cccgctctac cgcaacgccc ttctattaac aggacttttg ctttccggaa
    12121 tagccgcagt tcaggccgcc gactggccgc gtcagattac tgacagccgg ggcactcata
    12181 ccctggaaag ccagccgcag cgtattgttt ccaccagcgt caccctgacc ggctcactgc
    12241 tggcgattga tgctccggtg attgccagcg gcgcgaccac gccaaataac cgcgtcgcgg
    12301 atgaccaggg ctttttacgc cagtggagca aggtggcgaa agaacgcaaa ctacaacggc
    12361 tctatatcgg cgaaccgagc gccgaagccg ttgccgcgca aatgccggat ctgattttaa
    12421 ttagcgcaac cggcggggat tcggcgctgg cgctgtatga tcagctttcc accatcgccc
    12481 cgacattaat catcaattac gacgacaaaa gctggcagtc gctgttaacg cagcttggcg
    12541 aaattaccgg gcatgagaaa caagcggcag agcggattgc acagtttgat aagcaactgg
    12601 cagcggcgaa agagcaaatc aaattaccgc cgcagccggt cactgctatt gtctataccg
    12661 ccgctgcaca cagtgccaat ctctggacgc cggaatcagc acaagggcag atgctggaac
    12721 aactcggctt tacgctggcg aaattgccca cagggttaaa cgccagccaa agccagggca
    12781 aacgccatga catcattcag cttagtggag aaaatctggc tgcagggtta aatggcgagt
    12841 cactattcct gttcgccggt gatcagaaag acgccgatgc gatttatgcc aatccactgc
    12901 tcgcgcacct gcccgcagta caaaacaagc aggtttatgc gctgggaacc gagacgttcc
    12961 gcctggatta ctacagcgcc atgcaagtgc tggaacgact taaggcgctg ttttaagcat
    13021 taactgtcgg acgctgtcac ctgcggcggc gtctggcgaa aacgtcgcaa ctccaccagc
    13081 accagcaata acaacacgcc gataatcaac aaaccaaaac cgctcgcgct tgcggaagca
    13141 accggtgtca tcatcgctcc cagaccgcct aacagcgccg cgcctatggc atcgcccgtc
    13201 acgttttgtg ccgtccacaa accgttgatt cgccctaaca ttgcttccgg cgtttgcgtt
    13261 tgcagcattg tatattgcag caacgaactg accgcactca gccagccgaa cagcgccaga
    13321 caaaccacgc ctaaaatcca catcggcatc aggccaaata aaccaatggc gaggaacgat
    13381 cccaacgtgg agagcaacat caacaatcct ggtcgcgcgc tatgcgccag tttcccactg
    13441 gttaatgcgc caatagccgc accgagcggg atcgccgcat agagaaaacc aatctctgcc
    13501 gccgacatct gccagttgtc agccagcgcc ggatacagta cccgcaccgc gctcgccatc
    13561 gtcaataaac cacccagcaa cgcaatcccg cccaccagcg ggctggcgag cagaaaacga
    13621 aatcctgcca gtaatgattt caacggatgc tcgcgcggct gcggtggcgg tggcaacgcc
    13681 ggaaggctta acaacggtag caaggtaata aacgtgcccg ccgccgccag cccgtagttc
    13741 caggctacgc caccggtcgc cagcaataaa ccgccaatca tgggcgaaat caccgacccc
    13801 agacgcacgg tcaacatggt gatcgccccg gcctgcatta agttttcacg ccctaccagt
    13861 gctggtgtcg ccgccaatag cgccgtaacg ccaagcgatg cgaaaaaacc atcccataaa
    13921 ccaagtaaat agattgccag caatgacggc tccggcagca gtgcattaag gcacagtcca
    13981 atgaagccaa tgccacaggt gccgcgcgcc agcaaaatca cttttttgcg ctcatagcga
    14041 tccgccagca caccgccaac catcaggcca acaaacatcg cgccgccggt cagcgtcacc
    14101 gaaagcccca ccagccaggt agaatgcgtc atcatctgga tctgcaccgg caccgcgacg
    14161 ccgagcaaac ccagagacac aattgagata aaacgagcga ggaatactgc gcgaaacgcc
    14221 gggtgcgttt tcaacaggct gaggttaagc agccaggatt gtttattcat tacaatgcct
    14281 tgccatcaaa tattttaaat acctattctt aggctgcaca tgctaacata tccaaataag
    14341 atcgataacg ataattaatt tcattatcat ggaagttcgt atgtctggtt ctgttgccgt
    14401 gacacgcgcc attgccgtgc ccggattgct gttattactg attatcgcga cggcattaag
    14461 cctgctcatt ggggcaaaat cactccccgc ttccgtagtg ctggaggcct tctccggcac
    14521 ctgccagagc gccgactgca ccatcgtgct cgacgcgcga ctgccgcgca cccttgccgg
    14581 tttactggca ggcggcgcgc ttggccttgc cggggcgttg atgcaaaccc tcacccgaaa
    14641 tccacttgcc gaccccggct tgcttggcgt gaacgccgga gccagctttg ccattgtgct
    14701 gggtgcggcg ctgtttggtt actcatccgc gcaggaacaa ctggcgatgg ccttcgccgg
    14761 ggcgctggtg gcctcattga ttgttgcctt taccggcagt cagggcggcg ggcagttaag
    14821 tccggtgcgt ttaaccctgg cgggcgtggc gctggcggca gtgctggaag ggctgaccag
    14881 cggcatcgcc ctgcttaatc ctgacgtcta cgatcaactg cgtttctggc aagccggttc
    14941 gctggatatt cgcaatctac ataccttaaa agtggtgctg atcccggtgc tgatcgccgg
    15001 agcaaccgcg ctattactga gccgcgcgct gaacagttta agcctcggca gcgacaccgc
    15061 gacggcgctg ggcagtcgcg tggcgcgcac acagttaatt ggtctgctgg cgattaccgt
    15121 gctttgtggt agtgcgacag cggtagttgg cccgattgcc tttattggcc tgatgatgcc
    15181 gcatatggcg cgttggctgg tgggtgccga tcatcgctgg tcgctgcccg taacgctgct
    15241 tgctacccct gccctgctgc tgtttgccga tatcatcggg cgggtgattg tgcccggtga
    15301 actgcgcgtt tctgtggtca gtgcctttat tggtgcaccg gtgctgatct tcctcgtgcg
    15361 acgtaaaacg cgaggtggtg catgatttac gtctctcgcc gattactcat cacctgtttg
    15421 ctgcttattt ccgcctgtgt ggttgcaggt atctggggat tacgcagcgg tgccgtcacg
    15481 ctggaaacct cgcaggtatt cgccgcgctg atgggcgatg cgccgcgcag tatgacgatg
    15541 gtggtcaccg aatggcgttt accacgcgtg ctgatggcgc tgttgattgg cgcggcactg
    15601 ggcgtcagcg gtgcgatttt tcagtcgctg atgcgtaacc cgctcggcag ccctgacgta
    15661 atgggcttta acaccggggc gtggagcggc gtgctggtgg cgatggtgct gtttggtcag
    15721 gacctgacgg ctatcgcgct ggcagcaatg gtgggcggca ttgtcacttc tctgttggtc
    15781 tggctgctcg cctggcgtaa cggtatcgac acctttcggt tgattattat cggcatcggc
    15841 gttcgcgcca tgctggtggc ctttaatact tggctgttgc tgaaagcgtc tttagaaacg
    15901 gcgctaacag cgggtttgtg gaatgccgga tcgctcaatg gcctgacgtg ggcaaaaact
    15961 tcgccttccg cacccatcat tatattgatg ctcattgccg ccgccttgct ggtgcggcga
    16021 atgcgcttgc tggagatggg cgatgatacc gcctgcgcgc tgggcgtcag cgtcgagcgt
    16081 tcgcgtctgt taatgatgct ggttgcagtg gtgcttaccg ctgcggcaac ggcgcttgcc
    16141 gggccgattt cctttattgc tttagtcgcg ccgcacattg cccgacgcat tagcggcacc
    16201 gctcgctggg ggctgaccca ggcggcgctg tgcggagcgc tgttactgct ggcggccgat
    16261 ctctgcgccc aacaactgtt tatgccgtat caacttccgg ttggcgtcgt taccgtcagc
    16321 ctcggcggta tttaccttat cgtcttgtta attcaggagt ctcgcaaaaa atgaccgaat
    16381 cagtagcccg tttgcgcggc gaacagttaa ccctgggata tggcaaatat accgttgcgg
    16441 aaaatctgac tgtagaaatt cctgatggtc acttcacggc aattatcgga ccaaatggct
    16501 gtggtaaatc cacgttactg cgtaccttaa gccgcctgat gacgcctgct cacgggcatg
    16561 tctggctgga tggcgagcac attaaacatt acgccagtaa agaagttgca cgccggattg
    16621 gtctgttggc gcaaaatgct accacgccgg gcgatatcac cgtgcaggag ctggtggcgc
    16681 gtggacgtta tccgcatcaa ccgctgttta cccactggcg caaagaggat gaagaagcgg
    16741 taacgaaagc gatgcaggcc acgggaataa ctcatctggc agatcaaagc gtggataccc
    16801 tttctggcgg acaacgccag cgagcgtgga tcgcgatggt gctggcccag gaaacggcaa
    16861 ttatgctgct cgacgaaccc acgacctggc tggatatcag tcatcagatt gatctactgg
    16921 aactgttaag cgaactgaac cgcgagaaag gctataccct ggcggcggtg ctgcacgatc
    16981 ttaatcaggc ctgtcgttac gccagccatt tgattgcatt gcgggaaggg aaaattgttg
    17041 ctcagggagc gccgaaggag attgtcactg ctgaactgat tgagcgcatt tatggtctgc
    17101 gatgcatgat cattgacgat ccggtagccg gaacgccgct tgtggtgccg ctcgaacgaa
    17161 cggcaccttc aaccgcaaat agttaaacta agtggtctgc catcatggca tcctgttttc
    17221 tggatgccat cgcatagcgc aataacacgc taccacaagc caccatcccg ccgatcagcg
    17281 cggaaaggat cacaataatc gccttacccg gaccgtcttt tttcactggc aatgacgggc
    17341 ttaactgata tttaaacggc gtaaaattca catcgttgac atttgctttt gttaattgct
    17401 cgacaagata ctgccgatta cgtaattcac cgttcagttc cgcaacgtca gtaaccgctt
    17461 tttcaatttc cagtttgcgt tcaataccgt ctgcgccgag agaaatagag aaatcggggt
    17521 catctttaac ggcctgacca ttactgtata cgggcttttt aattcctgcc gcgttggcaa
    17581 tgtcgagtga ataattgagg cgctgaatgt ttgcatcaag ttgatttttc gttttaatgc
    17641 gatcctgagc cagtttttct ttttcaaact gggttttgat ctccagttta ttacggacgt
    17701 tttctatcga ctctttcacc accaacgcag agatataatc gatatacccg ctcaaaacgg
    17761 tctgcgcctc tttactggtt ggcgcggtaa aacttagcgt ccaggaggta tacagtgacg
    17821 gttcatcttt tttcttactg gcattgtcat caaccgcttt cattttttcg ctcaatgcaa
    17881 caatagcgcg atgcaaatcc agttcatcga ttttcgcctc ttttaattgg tccatcacat
    17941 aaggtgatga acgcaggtac tcttccagca agctaacaga ctgaaacttc ttgataaaca
    18001 ggttaaatgc accgccacgg tcgatattaa catcgagatc aagaacgcgg agtttggtaa
    18061 aggttttctc caggtcctgc cactgaatgg cttcggcggg agtaataacg gctgagctgg
    18121 tccatttttg cggcaggatg aaagagatca gtaaacctgc gcaggcaaat gcaaaaacga
    18181 ccgccatgac cgtttttttg gcccgccata aaacttcgat tagattaagt aaatcaattt
    18241 cattactact gggcgacgcc agaggatact cggaaaaatg agcgtcactt ccctgtttaa
    18301 tattcagtga tgacatgggt ataacctgaa aatgagtcca atagggggaa atctccacgg
    18361 cgctcacttt accagttggt gagaattatc cgaagctgaa gtttgtaaat tccttccact
    18421 gaatgcaaca ttcactctat ttaagagaaa tattcatcaa acctataaat caaacaaata
    18481 aaaaacccat aattattttc tgtaattatg ggtttataga taatattaat ttacctgttt
    18541 agcgttgcgc gaataatcgg cccaattttt acaaacgccc ctggagagat aatatccaca
    18601 tgcgcacaat cctgacgata gatatccagc tcggcaatcc agggcgacca agcgcgttcg
    18661 gggctcatac cttcctgaag cgtgcgttca gcaacaaaca gcgtcgcttt accatcaaac
    18721 ggtacgctat gagcagtcgt cagcagacgc acagcatcag cgtagttgcc ttcaatggtg
    18781 gtaaacaact ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgttcgcgg
    18841 ttaatctccg ccagcacttc cgggtccaga ccattagcct ctttttcctg ccagttttgc
    18901 gtttctggcg gccaggtatc cagcaaacca agaaatgcca cctgttcgcc acgggcacgc
    18961 agtcgcgccg caatgccctg cgccagcgta ccgccaaggg aataccccag caggtaataa
    19021 gggccgtgcg gttgttgttc aagtaacgtt gccagatgcg cttcacagac ttcatccaga
    19081 tttgccgcgg tatgcatggg gccattgggg cgcggcgact gaatgccgat aatcgaccat
    19141 tgtggatcga tataacgcga gagcacgcta aactgccagg caaaaccgga tgccggatgg
    19201 aaacaaaaca gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccata
    19261 cgccgggtgc tgtcttcttc agcatcaata atcgttgcca gtttagcgac ggttgacgcg
    19321 accatcactt gccccggcgt cacctggcgg gcaacctgcc gacttaactg cgctgccagt
    19381 ttcattgcca gtagcgaatg accaccaagc gcaaagaaat cagcatcggc atcctgcacg
    19441 tcacaaccca gcaacgacgc gaatgccgcg gcaataatcg tttcactgcc cgcttttggc
    19501 gcacgccccg gcgcttgtgc cttcagttca ggcaacggta aggctttgcg atccagcttg
    19561 ccgttggcgc taagaggtaa cagtggaagt tgcagcagaa ccacgggcac catatgtggt
    19621 ggcaatgttt cgcgaagctg cgcctgtaat gcgctggtat ccaacggcag acctgattgc
    19681 gacaccagat agcccaccaa ctgacgcgca tcaccaccgg tggctgccgc ctggttaatc
    19741 acacaggcgt gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt
    19801 tcgcccagtt caatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac
    19861 tccaccgcgc cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcacca
    19921 gggacaaaag gatcggcaat aaagcggctg gcggtcagat cgggtcgtcc aagatacccc
    19981 tgcgccagtt gaatgccggt gagatagaga tcacccgcca cacccggcgg caccggatgc
    20041 atcatcgcat caagaatacg cagacccgta ttccataccg gataaccaat cggcacactg
    20101 ctgccgcgca cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc
    20161 gcttccgtcg ggccatatag attatgcaac ggcacgctgg ttaactgttg ccattcgcgg
    20221 cataaatcag ccggtaaggc ctcaccacta cagaaaacct gtttcaacgt cgcgcaactc
    20281 tggcgagcgg tttgcggcgt cagcgatgca acaaatgctg ccagcatcga cggcacaaag
    20341 tgcgtggtcg ttacgccata ttcggcaaag aattgctgca tagcgagcgg gtcgcggtgc
    20401 gcttccggtt cagccatcac cagtttagcc ccggcaataa acggccagaa aaactcccac
    20461 accgagacat caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcaga
    20521 taatgatttt gcatccacaa caggcggtta acgatagccg tctgcccgac cattacccct
    20581 ttcggcctgc cggtggagcc agaggtaaag atgatataag ccgtgtgatg cggttgtgaa
    20641 agttgcagcg gcgcactgcc ctgcggttta agcggggcgt tatagcaaag gttggttaaa
    20701 tctggaacat cggcaaagcg cggcagttga tcgtcggtgg tgattaacag cgacggacgc
    20761 gcatcttcca gcatcatttt caggcgatcg tccggatagc cggtatccag cggtagccag
    20821 gccgcgccag cttcaactat cgcatgtagc gccagggtca aaaagaccga gcgcggtagc
    20881 gccaccgcca cgctgtcgcc cggtttaacg ccacgctcac gcagcagatt cgccagcgcc
    20941 accacctgct cgcgcatttc ccgatagctg aactggtaac gcgcatctgc cagcgccgga
    21001 gcatccggtg tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc
    21061 tcaacctgag tggcgttgat ctgcgccagc tgcgcatact cacctggcag cataatatcg
    21121 acatcgccgc acaacagcgc cggatccgcg gcgaactggg caatcagcat tttcaggcgt
    21181 tcagcatgct ggattaacgt tggctcatcg taacgctgtt tattggcgag gatctcaata
    21241 ctcaaatcac cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt
    21301 gccaaggtat gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaataccttg
    21361 atattgagta ccggaccaaa cagcggttca tcacctgccg ctcgcccgct gtcacggaca
    21421 atttgttcgg catcgtaacg ttgatgacga cgcatttttt tcagttgtgc tgccagtcgg
    21481 gttgccagtt ccggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg caaaacgttg
    21541 agcacgggtc cggtagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatcca
    21601 gcggcgtagt ccatgcgatt gcacaatcgc cccagccaca aagccgtcag cgcaagggct
    21661 aaatcggtac gctgcacacc tgaaagttgc gtagccaact ggcggaattc cccgtcggta
    21721 aattccattt tcagacgtaa aatatctgcc gaagcgctgc gccccggtaa aggtgccgga
    21781 gaaagtgacg cgggcggcgg cagttgacga cgctgttctg cccagaatgc cgcatcacgc
    21841 tgccaggctt cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta
    21901 aacggcgaag caggcgttgg ttcaccacgc agccatgtgc agtaaatatt ggcgatctgg
    21961 cgggtaatgg ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag
    22021 taccagcggt tatccgccac ctgtatcagc tgatgaaaga ccagtggttt accgctatcg
    22081 acgcgcagat cttgttgcaa atccgcctgc attaatgcct gcgcagtacc gtgcggatca
    22141 atgttggttc gcaggtcgat aatttctggc agttcgaacg tcagcgcatc atcgacccac
    22201 tgccagactt cgccgttatc ttccgtaaaa cgcatccgca gcgtatctgc ttgcgctagt
    22261 ccgtcaacca ccgcgcgggc cagtaatggc gcatcaacct ctccggttaa ctcaacgtaa
    22321 tgcgctacgc tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc
    22381 tgtgcggcga ccaaaggtaa atgctggctc attgcgcctc ctgcaactgg gtgaaattcg
    22441 ccggtgtcag agtacgccag tgggcttcaa gccactgctg gcaggactct tgtgactgcg
    22501 gctgacacac aatgtcccag cctgccggta aggcgcattg ttgcggccac agactgaatt
    22561 gcccctgcgc attgcgcaaa atgtaaaacg ctccctgtgg atcatcgaag ggattactga
    22621 atgccatatt caactcctgt catggaaaag tggttgccag aggtcgatta gcccctgcat
    22681 caagccaccg cgccaacaaa gcgcatcatg tccgccgtca acctggcgcc agaaaatgga
    22741 ttcttttatg gggtgtaatt gcgcatacag cgcctgattg gcccgcataa tcatcggctc
    22801 gcgaataccc gcttccagca caatgcgcag accttcggcg ctaacttcac cagctttaag
    22861 cttttcaagt aacacgccct cttgctgccc gccccgatgc ggccaccagt acgatcctga
    22921 ctggcttaat acacaaccaa aacgttcagg ccagtgcagt ccggcataca gcgcggaaag
    22981 tccaccaaaa ctctgcccgg caaccacggt gcgatcggcg cgatcgctaa aaggggcaat
    23041 agctttcacc aggggtaata actcttgctg tactgcgagc cagaaatccg cattacacgg
    23101 cagttcgtga gcgcggtgcg tggtgtcgat agcgtcgatc aacacataca cggcgggagg
    23161 aagttgctga cgatgggtca gcgaagtcag cactggccag acgggcatac tttgcgccca
    23221 aaattcgcca tcgagcaaaa ctgccagcgg gcgttcttct gctgttgcat cgccggtggt
    23281 aaaaatccat acacgacgtg aatttttcaa ccgttcactt ttccagataa tttctttggc
    23341 tggtatttct ggcgcttgcg gacaatccca tcccggttgc agaggcgctt gcggcatttc
    23401 gagtgcagaa acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc
    23461 tattgcctgg ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg
    23521 tgcagaaaaa atgtcatcgc gttcggtggg aataaagcag tagctgccgc gccagttggc
    23581 attgagttgt gtcgtccact gccagacatc agtacctgca atgcgctgca tcgactgggg
    23641 ctggctgttc tgatgatgat cggtcacacc agtgatgtag acccatacgc gctttatcgg
    23701 cgagtattct tcagaacctt ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc
    23761 attcagacgc tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt
    23821 taacgccgtc acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata
    23881 tattgataat attattgata actatttgca tttgcaatag cgtaatggcg cgccgtggga
    23941 agcgcggaca ttaattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg
    24001 tagcgggatg cgtcgtgttg aggacgacca tgcccgacag ttgcaattcg tggcaaaaat
    24061 gcaggaataa aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga
    24121 tttatggggt agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca
    24181 ttgtcgttac cgccgccgag cagaacttgc aggcacctgg cgtttcgacc atcaccgcag
    24241 atgaaatccg caaaaacccg gttgcccgcg atgtgtcgga gatcatccgt accatgccag
    24301 gcgttaacct gaccggtaac tccaccagtg gtcagcgtgg gaataaccga cagattgata
    24361 ttcgcggtat gggtccggaa aacacgctga ttttaattga cggcaaaccg gtcagcagcc
    24421 gtaactcggt gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acttcctggg
    24481 tgccgcctga aatgattgaa cgtattgaag ttctgcgtgg tccggctgca gcgcgttatg
    24541 gcaacggcgc ggcgggtggc gtagttaaca tcattaccaa aaaaggcagc ggtgagtggc
    24601 acggttcctg ggacgcttat ttcaatgcgc cagaacataa agaggaaggt gccactaaac
    24661 gcaccaactt cagtctgacc ggcccgctgg gcgacgaatt cagcttccgc ttgtatggca
    24721 acctcgacaa aacccaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg
    24781 ccggaacgta tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaac aaagatatta
    24841 atggcgtggt gcgctgggac ttcgcgcctc tgcaatcact cgaactggaa gcgggttaca
    24901 gccgccaggg taacctgtat gcgggcgata cccagaacac caactctgac gcttacactc
    24961 gctcgaaata tggcgatgaa accaaccgcc tgtatcgcca gaactactcg ctgacctgga
    25021 acggtggctg ggataacgga gtgaccacca gcaactgggt gcagtatgaa cacacccgta
    25081 actcgcgtat tccggaaggt ctggcgggcg gtaccgaagg gaaatttaac gaaaaagcga
    25141 cacaggattt cgtcgatatc gatcttgatg acgtgatgct gcacagcgaa gttaacctgc
    25201 cgattgattt cctcgttaac caaacgctga cgctgggtac ggagtggaat cagcaacgga
    25261 tgaaggactt gagttccaat acccaggcgc tgaccggggc gaacaccggc ggcgctattg
    25321 atggtgtgag tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctatttg
    25381 ccgaaaacaa catggagctg actgacagca ccatcgtaac gccggggctg cgtttcgatc
    25441 atcacagtat tgtcggcaat aactggagcc cggcgctgaa catatcgcaa ggtttaggcg
    25501 atgacttcac gctgaaaatg ggcattgccc gcgcctataa agcgccgagc ctgtaccaga
    25561 ctaacccgaa ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct
    25621 gctatctgca aggtaacgat gacctgaaag cagaaaccag catcaacaaa gagattggtc
    25681 tggagttcaa acgcgacggc tggctggcgg gcgtcacctg gttccgtaac gattatcgca
    25741 ataagattga agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc
    25801 agtgggataa cgtgccgaaa gcagtggttg aaggtctgga aggatcgtta aacgtaccag
    25861 ttagcgaaac ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa
    25921 ccacgggcga ccgtttgtcg atcatcccgg agtacacgtt gaactcaacg ctgagctggc
    25981 aggcgcggga agatttgtcg atgcaaacga ccttcacctg gtacggtaag cagcagccga
    26041 aaaaatacaa ctacaaaggt cagccagcgg ttggcccgga aaccaaagaa atcagtcctt
    26101 acagcattgt gggtctgagc gcgaccaggg atgtgacgaa gaatgtcagt ctgaccggcg
    26161 gcgtggacaa cctgttcgac aaacgtttgt ggcgtgcggg taatgcccag accacgggcg
    26221 atctggcagg ggccaactat atcgcgggtg ccggggcgta tacctataac gagccgggac
    26281 gtacgtggta catgagcgta aacactcact tctgatgcta acgtcagatt gttgataaag
    26341 tgcgcgtcgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa aaatcttgcc
    26401 aattcaatat attgcaggac catgtaggcc tgataagcgt agcgcatcag gcagtttagc
    26461 gtttgtcacc agtctctaat atggtcgaca tgaaaactac gcatacctcc ctcccctttg
    26521 ccggacatac gctgcatttt gttgagttcg atccggcgaa tttttgtgag caggatttac
    26581 tctggctgcc gcattacgcg caactacaac acgctggacg gaagcgcaaa gccgaacatt
    26641 tagccgggcg gatcgctgca atttatgcgc tgcgggaata tggctataaa tgtgtgcccg
    26701 caatcggcga gctacgccaa ccggtgtggc ctgcggaggt atacggcagt attagccact
    26761 gcggggctac ggcattggcc gtggtttccc gtcaaccgat tggcgttgat atcgaagaaa
    26821 ttttttctgc gcaaacagcg accgaattga cagacaacat tattacacca gcagaacacg
    26881 agcgactcgc agactgcggt ttagcctttt ctctggcgct gacactggca ttttccgcca
    26941 aagagagcgc atttaaggca agtgagatcc aaactgatgc aggttttctg gactatcaga
    27001 taattagctg gaataaacag caggtcatca ttcatcgtga gaatgagatg tttgctgtgc
    27061 actggcagat aaaagaaaaa atagtcataa ctctgtgcca acacgattaa ttgacaacat
    27121 ctggtacgat tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgcctt
    27181 accatcctga cctgacaacc ggatatgcgg ggattgctcc ccgcacaacg gctacttctt
    27241 cggttcgtaa gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca
    27301 cagcgaatcc ccgaccagaa gcgtaaatcc cagcaccgtt aaacacagca ctatgactgc
    27361 cgcaagggca tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat
    27421 ccaaattgtt caggtttggg taggtagccc cagggttgat agaaatatcg tcctggggct
    27481 tcatctttcc agacctcaga gtaaacctga aaccggaaag cttcaggcac ccgcagttat
    27541 cttacccttg cttagcaaaa tagctgttta tttatacagt aagtcctgtt tcggacttct
    27601 cactccccat ttgatcaact gaagctgaac ccgttttttt ctgcatgcct caatcgtcaa
    27661 aattcttcgc cacagtcagc actgccataa cttgtcacat tgcaaaagta gccaaattgc
    27721 ttctgtcata atcagttcac catcaccaaa aaaaagaaac attccagaac ttccgcatct
    27781 cgcccagccg ctcccacttc tatactgaat agaaaacgcc aacataagag aaacctatgc
    27841 cattacccga ttttcatgtt tctgaacctt ttacactcgg tattgaactg gaaatgctgg
    27901 tggttaatcc gccgggctat gacttaagcc aggactcttc aatactgatt gacgcggtta
    27961 aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg
    28021 cgacggatgt ttgccgtgat atcaaccagg ctgccgggca attttcagcg atgcagaaag
    28081 tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc acacacccgt
    28141 ttcagaaatg gcagcgacag gaggtatgcg ataacgagcg ctatcaacga acgctggaaa
    28201 actttggtta tctcattcaa caggtgaccg tttttggtca gcatgttcat gttggctgcg
    28261 ccagtggcga tgacgccatt tatttgctgc acggcctgtc acggtttgtg ccgcacttca
    28321 tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga tacgcgtttt gcctcctcac
    28381 gaccgaatat tttttccgcc tttcctgata atggcccgat gccgtgggtc agtaactggc
    28441 aacaatttga agccctgttt cgctgtctga gttacaccac gatgatcgac agcattaaag
    28501 atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt cgggtgatgg
    28561 ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag gctaccgccc
    28621 actggttact gacagaacgc ccgttcaaac atcaggagaa agattacctg ctgtataaat
    28681 tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt cattaccgat ccgcacactg
    28741 gcgatcgtcg accgctaacg gaagacacct tgcgattgct ggaaaaaatc gccccttccg
    28801 cacataaaat tggtgcatcg agcgcgattg aggccctgca tcgccaggtc gtcagcggtc
    28861 tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg attgggctgg
    28921 tgaaaaagca ttgtgagatc tgggccggtg actaagtagg gaattgcctt acagaagaat
    28981 atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc ctttagaaac
    29041 cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc
    29101 ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc tgatggatct
    29161 tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg ctattgagta
    29221 ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa cgtcgattat
    29281 cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg tttatggata
    29341 agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt gtcgagcttt
    29401 gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt tttatgctgt
    29461 cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa aaatccctgt
    29521 taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa aagcactgga
    29581 tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg atcaacgatt
    29641 cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg cgtccaggct
    29701 aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac cttgtaatct
    29761 gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct gtcgccttaa
    29821 agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa caggccgagc
    29881 agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg tggcatttta
    29941 ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg
    30001 tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg
    30061 cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat ggccgctttg
    30121 ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct gatatgcacc
    30181 gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc aacgtcggta
    30241 acttcctgct cggcgtggcg gctctgggtc tggacgcggt acccatcgaa ggttttgacg
    30301 ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt ctggtggttg
    30361 ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgcgaaat ctcgtctgcc
    30421 gcaaaacatc accttaaccg aagtgtaatt ctctcttgcc gggcatctgc ccggctattt
    30481 cccctcagat tctcctgatt tgcataaccc tgtttcagcc gtcatcatag gctgctgttg
    30541 tataaaggag acgttatgca ggatttaata tcccaggttg gggatttagc gggtattgag
    30601 atcgatcaca ccacctcgat ggtgatgatt ttcggtatta tttttctgac cgccgtcgtg
    30661 gtgcatatta ttttgcattg ggtggtactg cggaccttcg aaaaacgtgc catcgccagt
    30721 tcacggcttt ggttgcaaat cattacccag aataaactct tccaccgttt agcttttacc
    30781 ctgcagggga ttatcgtcaa tattcaggcg gtattctggc tgcaaaaagg caccgaagcg
    30841 gcagatattc tgactacctg cgcgcagttg tggatcatga tgtatgcgct gctttcagtc
    30901 ttctcgttgc tggatgttat tttgaatctg gcgcagaaat tcccggcggc atctcagtta
    30961 ccgctgaaag ggatatttca ggggattaaa ctgatcggcg caatcctggt cggcattttg
    31021 atgatctcgc tgctgattgg tcagtcgcca gcgattctga tcagcggtct tggtgcaatg
    31081 gctgccgtgc tgatgttggt atttaaagat ccgattcttg gtctggtggc aggtattcag
    31141 ctttccgcga acgatatgct gaaactgggc gactggctgg agatgccgaa atacggcgcg
    31201 gatggcacgg tgatcgatat tgggttaacc accgtcaaag tgcgtaactg ggacaatacc
    31261 attaccacta ttcccacctg gtctctggtt tctgactcct ttaaaaactg gagcgggatg
    31321 tcagcatctg gcgggcgacg tattaagcgc agtatcagta ttgatgtcac cagtattcgt
    31381 tttcttgatg aagacgaaat gcaacgtctg aataaagcgc acttgttaaa accttattta
    31441 accagccgcc atcaggaaat taatgagtgg aatcgccagc aaggttctac ggagtcgata
    31501 ttaaacctgc gccgaatgac caatattgga acctttcgtg cctatctgaa cgaatatctg
    31561 cgtaaccatc cgcggattcg taaagatatg accttaatgg tacgccaact ggctccaggt
    31621 gataacggtt taccgctcga gatctacgcg tttaccaaca ccgtggtgtg gctggaatat
    31681 gaaagcattc aggctgatat attcgatcac atatttgcga ttgtcgaaga gtttggtctg
    31741 cgacttcatc agtcgccagc cggcaatgat attcgctctc tggcgggtgc atttaagcag
    31801 taattaaaaa aaccgctctc atcgaatgga tgagagcgga catcgttttg tcggatgtag
    31861 cgtgaatgcc ttatttccga cgcagcgttt taaatgccac aaacaggaat acaatccaca
    31921 ccggcagcag gatcgctgac aagcgcatat catccatcgt gcacatcagc agcaaaatca
    31981 tgccgaggaa ggcaatgcaa agatagttgc cgaacggata aagcagcgcc ttaaactgtg
    32041 tttcacgtcc ctgacgtcgc atcgccgcgc gaaaacgcag atgcgccagg cagatcataa
    32101 tccagttcaa cagcagcgtt gctaccacca gcgccatcag cagaccaaac gctttttgcg
    32161 gcagcagata gttgattaac accaccagcg aagtgatcgc tccggaaagc atcagcgagt
    32221 taatcggcac gccgcgatgg ctgacgcgag tcaaaaactt cggcgcatta ccctgcacag
    32281 aaaggccaaa cagcatgcgg ctgttagagt aaaccccgct gttatacact gacagcgatg
    32341 ctaccagaat gacgaagttc agcgcagaag ctacctcgtt gctgtcgaga ttatggaaaa
    32401 tcatcacaaa cgggctactg ttggatttca cttccaccca cggatagagc gccagtaaaa
    32461 ccaccagtga acagatgtaa aacagcagga tgcgatacac cacctgattt accgcttttg
    32521 gaatgctttt ttccggatcg cgcgcttcag cggcagtaat cccaatcagc tccagaccgc
    32581 cgaaggagaa cataattacc gccagcgaca aaatcagccc attccagccg gtggcgaaga
    32641 aaccaccgta gcgccagagg ttgtcgatac tggctttctc gccgccgtga ccagaaaaca
    32701 gcagccacag gccaaagccg atcataccga tgattgccag cactttaatc aacgcaaacc
    32761 agaactcggt ttcgccatat aagcgcacgt tcaccaggtt aacggcgttg atgataataa
    32821 agaaggcggc agcccaaatc cacgttggaa catccgggaa ccagtactgc atatagatgc
    32881 ccgcagcggt cagctctgcc attcccacca gcacgaacat tacccagtag ttccagccag
    32941 agaggaagcc cgcaaacggt ccccagtatt tataggcaaa gtgggcaaat gaaccggata
    33001 ccggctcttc gaccaccatc tcgccgagct ggcgcataat caggaaagcg atgatcccgg
    33061 cgacgccgta gcccagcaat acagccggac ccgccatctg aatcgccggg ccaatgccaa
    33121 gaaacagacc agtaccaatt gcgccaccca gcgcaatcag ttgaatatga cggttatgta
    33181 atccgcgatg aagcgtcggc tcttgatttg acgcagtatc ttccgatacg gttgacgcgt
    33241 ttttcacgcc tttcccctgt gtgtcttttt tgttgagggg caccttttaa catttagtgc
    33301 ccatcgtcgc aagacacaat ccacacggtt aaacggggta tcctgctttt atttccgtac
    33361 ccgatgtcgg tgcagccaca tcagcttata cgccgccggg ataataaaca gcgacagcaa
    33421 aggcgcggtg atcatgccgc caatcatcgg tgcggcaatc cggctcatca cctctgaacc
    33481 agcccccgtt ccccacagaa tcggcagcag accggcgata atcaccgcca ccgtcatcgc
    33541 tttcgggcgc acgcgcagta ccgcgccgtg atacaacgcc tcatccagct tctgctcact
    33601 gaatgtttgc ggattattca acgacggctc ggcctctatg gcgtgacgta aatacatcag
    33661 catcaccacg ccaaattcgg cggcgccccc ggcaagggca ataaaaccgg tgcccgttgc
    33721 cacagaaaga tgaaagccca tccaccacag caaccagatg ccgcccacca gcgcaaacgg
    33781 tacgctgctg ataatcagca acgcttcgcc cacccgacgg aatgccagat acaacagcac
    33841 gaagatgatc atcagtgtca tcggcaccat gagtttcagc ttatggttgg cgcgctccag
    33901 tagctcgaac tgcccggaga atgccacgct ggtgcccggt tttaactgca ctttttcaac
    33961 tatcgctttt tgcaaatcgt gaaccaccga caccatgtca cgatcgcggg catcgatata
    34021 aatccagctc gtcgggcgcg cattctcggt tttcagcatc gacggtccgg tagagacttt
    34081 aacgtcggcc acgtctgcca gggtgatttg ctgcttcatc ggcgtcagga tcggtagctg
    34141 gcgcagtgcc tgcgggctat cgcgccagct ttgcggataa cgcagattaa tgggataacg
    34201 ggcaatccct tccaccgttt cgccaaccat cgccccgccc accgcagaag tcacaaacaa
    34261 ctgcacatcc gccaccgtca taccgtaacg cgcggctttt tcacggttaa tctcaacgtt
    34321 gatatagcgc ccaccttcca gacgctcggc aagcgcagaa gccacgcctg gcaccgttcg
    34381 cgctacttct tcaatttgct cagccatcgc gtcgatatcc gccagcacag tgccggaaac
    34441 tttaatgccg atggggcttt taatgccggt tgagagcata tcgatacggt tacgaattgg
    34501 cggcacccac agattcgcca gccccggcag ccgcacggtg ttatccagtt cctcaatgat
    34561 tttgtccatc gtcatgccag gccgccactg atcctgcggc ttaagctgga tggtcgtttc
    34621 aaccatctcg agcggagcag aatcggtggc ggtttccgct ttcccggttt tgccaaatac
    34681 ccgcgccact tcaggtacgc tcataattag cttgtcgttt ttttgcagca tactcgccgc
    34741 ctctgctgcg gaaatccccg gcagcgtcga tggcatatac aacaagtcgc cttcattgat
    34801 ctgcggtaaa aattccccgc caactttatt gagcggccag agaaccgtca gcaccgaaag
    34861 cgctgccacc aacagcgtgg ttttcggcca gtgcagtact ttcagcaata gaggatgata
    34921 aacacgaatc aaaaagcgat tgagcgggtt actgctttcc ggcggaattt tgccacggat
    34981 ccagtaaccc atcaggatcg ggatcactac gatcgccagc agcgccgcac ccgccatcgc
    35041 atacgttttg gtgaacgcca gcgggccaaa cagacgccct tcttgccctt ccagggtgaa
    35101 gattgggata aacgacagcg tgataatcag cagactgata aacagcgccg gccccacttc
    35161 aacagatgca tcggtaatca cctgccagcg cgttttatta tccagcgtgg cgtcaggatg
    35221 ctggtgctgc cactcttcca gccgtttatg cgcattctcg atcatgacga tagcagcatc
    35281 gaccatcgcc ccgacggcaa tcgcaatgcc acccaacgac ataatattgg cattcagtcc
    35341 ctggaagggc atgacaataa aagcaataca caaccccagc ggcaacgaaa taatcgccac
    35401 cagcgccgag cgcacatgcc agagaaacag cgcacagact accgccacca caataaactc
    35461 ttccagcaac ttgccgctga ggttgtcgat ggcgcggtca atgagctggc tgcgatcgta
    35521 tgttgtaact atctccacgc cttccggcag actacttttc agcgtttcca gtttgtcctt
    35581 cacggcggcg atcacttctc gggcgttttt gccggagcgc aggatcacca ccccgcccgc
    35641 cacttcgcct tcgccgttca gttcggcaat gccccggcgc atctccgggc caacctggac
    35701 cttcgcaaca tcgcgcagat aaacgggcac gccattttca ctggctttta aaacgatgtg
    35761 attaaagtcg tcgagcgttt gcagatagcc gctggcgcgc accatatatt ccgcttccgc
    35821 cagttcgatc gacgaaccgc ccgcttcctg gtttgaagca tccagcgcgc tttttacttc
    35881 ggcgagactg atgccatact gcgccaggcg ctggggatcg ataaccacct gatactcttt
    35941 caccacaccg cccaccgacg ccacttccgc aacgtcaggg atggttttca gctcatattt
    36001 gagaaaccag tcctgtaatg agcgtaaatc ggccagatcg tgcttaccgc tgcgatccac
    36061 cagtgcatat tcatagatcc agccaacacc cgtggcatct ggccccagct cggcgctgac
    36121 tcccgcaggc agcttaccct gtacctggtt gaggtactcc agcacccgcg aacgcgccca
    36181 gtacggatcg gtaccatctt cgaaaatgac atacacataa gagtcgccaa actgcgagaa
    36241 accacgcaca gtcttcgcgc caggcaccga caacatggtg gtggttagcg gataagtcac
    36301 ctgattttca acgatttgcg gtgcctgccc gggatagctg gttttaataa tcacctgcac
    36361 atcggagaga tccggcagcg catccactgg cgtattaatg atggtccagg tgccccagat
    36421 gctaagaaac aacgcgccca tcagcaccaa aaaacggttc gccaccgagc gacgaataat
    36481 ccattcaatc attggttatt ccctcaatgc gcatgggtag cactttcaga gcgcatccgc
    36541 tccagtgcgc cagaaatatt ggcttcggaa tcaatcagga aaaggccgct ggaaaccacc
    36601 ttttcacctt ccgccagacc agagcgtaat gcggtgacac cttgcgatgc ctggaaaacg
    36661 gcaacgcgtt tcggtacaaa gcgcccgtcg gcatcaatgg taatcacccg ctgttcgcta
    36721 ccggtatcaa tcagcccttg tgacggaatg agcagcatcg gttcgctggc ggtattgagt
    36781 tgcaaccagg cgttcatgcc cggttttagc gcctcgtcgg cgttgtcgac ttccagacgc
    36841 agctgcagcg tgcgggtcgc ggcatccacg ccgggtagca gcgtccattt gcggatggtg
    36901 agtgttttat ccggtcgtgc cggaacggtg agcgtaaact gcgaggcatc tttcaccagc
    36961 caagcgatag actccgggat cgcagca
//
LOCUS       DAJVTY010000018        36504 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVTY010000018 DAJVTY010000000
VERSION     DAJVTY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36504
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.018"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            258..344
                     /gene="leuL"
                     /locus_tag="OQQ66_000854"
     CDS             258..344
                     /gene="leuL"
                     /locus_tag="OQQ66_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1903871.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            437..2008
                     /gene="leuA"
                     /locus_tag="OQQ66_000855"
     CDS             437..2008
                     /gene="leuA"
                     /locus_tag="OQQ66_000855"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1903872.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2008..3099
                     /gene="leuB"
                     /locus_tag="OQQ66_000856"
     CDS             2008..3099
                     /gene="leuB"
                     /locus_tag="OQQ66_000856"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1903873.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3102..4502
                     /gene="leuC"
                     /locus_tag="OQQ66_000857"
     CDS             3102..4502
                     /gene="leuC"
                     /locus_tag="OQQ66_000857"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1903874.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4513..5118
                     /gene="leuD"
                     /locus_tag="OQQ66_000858"
     CDS             4513..5118
                     /gene="leuD"
                     /locus_tag="OQQ66_000858"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1903875.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5167..6249)
                     /locus_tag="OQQ66_000859"
     CDS             complement(5167..6249)
                     /locus_tag="OQQ66_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1903876.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMVVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6351..6482)
                     /gene="sgrT"
                     /locus_tag="OQQ66_000860"
     CDS             complement(6351..6482)
                     /gene="sgrT"
                     /locus_tag="OQQ66_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1903877.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6571..8226
                     /gene="sgrR"
                     /locus_tag="OQQ66_000861"
     CDS             6571..8226
                     /gene="sgrR"
                     /locus_tag="OQQ66_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1903878.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8260..8359
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8390..9373
                     /gene="thiB"
                     /locus_tag="OQQ66_000862"
     CDS             8390..9373
                     /gene="thiB"
                     /locus_tag="OQQ66_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1903879.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9349..10959
                     /gene="thiP"
                     /locus_tag="OQQ66_000863"
     CDS             9349..10959
                     /gene="thiP"
                     /locus_tag="OQQ66_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1903880.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10943..11641
                     /gene="thiQ"
                     /locus_tag="OQQ66_000864"
     CDS             10943..11641
                     /gene="thiQ"
                     /locus_tag="OQQ66_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1903881.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11754..12518)
                     /locus_tag="OQQ66_000865"
     CDS             complement(11754..12518)
                     /locus_tag="OQQ66_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1903882.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12604..13482)
                     /gene="araC"
                     /locus_tag="OQQ66_000866"
     CDS             complement(12604..13482)
                     /gene="araC"
                     /locus_tag="OQQ66_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1903883.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13821..15521
                     /gene="araB"
                     /locus_tag="OQQ66_000867"
     CDS             13821..15521
                     /gene="araB"
                     /locus_tag="OQQ66_000867"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1903884.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLNWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15532..17034
                     /gene="araA"
                     /locus_tag="OQQ66_000868"
     CDS             15532..17034
                     /gene="araA"
                     /locus_tag="OQQ66_000868"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1903885.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17234..17929
                     /gene="araD"
                     /locus_tag="OQQ66_000869"
     CDS             17234..17929
                     /gene="araD"
                     /locus_tag="OQQ66_000869"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1903886.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18004..20355
                     /gene="polB"
                     /locus_tag="OQQ66_000870"
     CDS             18004..20355
                     /gene="polB"
                     /locus_tag="OQQ66_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1903887.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20519..23425
                     /gene="rapA"
                     /locus_tag="OQQ66_000871"
     CDS             20519..23425
                     /gene="rapA"
                     /locus_tag="OQQ66_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1903888.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23437..24096
                     /gene="rluA"
                     /locus_tag="OQQ66_000872"
     CDS             23437..24096
                     /gene="rluA"
                     /locus_tag="OQQ66_000872"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1903889.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24213..25028)
                     /gene="djlA"
                     /locus_tag="OQQ66_000873"
     CDS             complement(24213..25028)
                     /gene="djlA"
                     /locus_tag="OQQ66_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1903890.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25283..27637
                     /gene="lptD"
                     /locus_tag="OQQ66_000874"
     CDS             25283..27637
                     /gene="lptD"
                     /locus_tag="OQQ66_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1903891.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27690..28976
                     /gene="surA"
                     /locus_tag="OQQ66_000875"
     CDS             27690..28976
                     /gene="surA"
                     /locus_tag="OQQ66_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1903892.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28976..29965
                     /gene="pdxA"
                     /locus_tag="OQQ66_000876"
     CDS             28976..29965
                     /gene="pdxA"
                     /locus_tag="OQQ66_000876"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1903893.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29962..30783
                     /gene="rsmA"
                     /locus_tag="OQQ66_000877"
     CDS             29962..30783
                     /gene="rsmA"
                     /locus_tag="OQQ66_000877"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1903894.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30786..31163
                     /gene="apaG"
                     /locus_tag="OQQ66_000878"
     CDS             30786..31163
                     /gene="apaG"
                     /locus_tag="OQQ66_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1903895.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31170..32012
                     /gene="apaH"
                     /locus_tag="OQQ66_000879"
     CDS             31170..32012
                     /gene="apaH"
                     /locus_tag="OQQ66_000879"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1903896.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32090..32569)
                     /gene="folA"
                     /locus_tag="OQQ66_000880"
     CDS             complement(32090..32569)
                     /gene="folA"
                     /locus_tag="OQQ66_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1903897.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32761..34623)
                     /gene="kefC"
                     /locus_tag="OQQ66_000881"
     CDS             complement(32761..34623)
                     /gene="kefC"
                     /locus_tag="OQQ66_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1903898.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34616..35146)
                     /gene="kefF"
                     /locus_tag="OQQ66_000882"
     CDS             complement(34616..35146)
                     /gene="kefF"
                     /locus_tag="OQQ66_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1903899.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35254..36504)
                     /locus_tag="OQQ66_000883"
                     /pseudo
     CDS             complement(35254..36504)
                     /locus_tag="OQQ66_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8817 a        10037 c         9654 g         7996 t
ORIGIN      
        1 tttgcaacgc gtgcattttg tccccttttt cctcgttgat tagatgcaaa aatttattct
       61 gaaatatgtc aacctatgaa aagtgtcggt cgttaaacag aaattaatat cgcttgcttt
      121 aaccaccgca gcacaattag ctaattttac ggatgcagaa ctcgcgctgg cgggacgttt
      181 ttattgcgtc agggttgaca tccgtttttg tatccagtaa ctctaaaagc atatcgcatt
      241 catctggagc tgatttaatg actcacatcg ttcgctttat cggtctacta ctactaaacg
      301 catcttcttt gcgcggtaga cgagtgagcg gcatccagca ttaagccagc acgcagtcaa
      361 acaaaaaacc cgcgccattg cgcgggtttt tttatgcccg aagcgaggcg ctctaaaaga
      421 gacaaggacc caaaccatga gccagcaagt cattattttc gataccacat tgcgcgacgg
      481 tgaacaggcg ttacaggcaa gcttgagtgt gaaagaaaaa ctgcaaattg cgctggccct
      541 tgagcgtatg ggtgttgacg tgatggaagt cggtttcccc gtctcttcgc cgggcgattt
      601 tgaatcagtg caaaccatcg cccgccaggt taaaaacagc cgcgtatgcg cgttagctcg
      661 ctgcgtggag aaagatatcg acgtggcggc cgaatccctg aaagtcgccg aagccttccg
      721 tattcatacc tttattgcca cttcgccaat gcacatcgcc accaagctgc gcagcacgct
      781 ggatgaagtg atcgaacgcg ctatctatat ggtgaaacgc gcccgtaatt acaccgatga
      841 tgttgaattt tcttgcgaag atgccgggcg tacccccatt gccgatctgg cgcgagtggt
      901 cgaagcggcg attaatgccg gtgccaccac catcaacatt ccggacaccg tgggctacac
      961 catgccgttt gagttcgccg gaatcatcag cggtctgtat gaacgcgtac ctaacatcga
     1021 caaagccatt atttccgtac atacccacga cgatttaggc ctggcggtcg gcaactcact
     1081 ggcggcggta catgccggtg cacgccaggt ggaaggtgca atgaacggga tcggcgagcg
     1141 tgccggtaac tgttcgctgg aagaagtcat catggcgatt aaagttcgta aggatattct
     1201 caacgtccac accgccatta atcaccagga gatatggcgc accagccagt tagttagcca
     1261 gatttgtaat atgccgatcc cggcaaacaa agccattgtt ggcagcggcg cattcgccca
     1321 ctcctccggt atccaccagg atggcgtgct gaaaaaccgc gaaaactacg aaatcatgac
     1381 accagaatct attggtctga accaaatcca gctgaatctg acctctcgtt cggggcgtgc
     1441 ggcggtgaaa catcgcatgg atgagatggg gtataaagaa agtgaatata atttagacaa
     1501 tttgtacgat gctttcctga agctggcgga caaaaaaggt caggtgtttg attacgatct
     1561 ggaggcgctg gccttcatcg gtaagcagca agaagagccg gagcatttcc gtctggatta
     1621 cttcagcgtg cagtctggct ctaacgatat cgccaccgcc gccgtcaaac tggcctgtgg
     1681 cgaagaagtc aaagcagaag ccgccaacgg taacggtccg gtcgatgccg tctatcaggc
     1741 aattaaccgc atcactgact ataacgtcga actggtgaaa tacagcctga ccgccaaagg
     1801 tcacggtaaa gatgcgctgg gtcaggtgga tattgtcgcc aactacaacg gtcgccgctt
     1861 ccacggcgtc ggcctggcca ccgatattgt cgagtcctcc gccaaagcca tggtgcacgt
     1921 actgaacaat atctggcgtg ccgcagaagt cgaaaaagag ttgcaacgca aagctcaaca
     1981 caacgaaaac aacaaggaaa ccgtgtgatg tcgaagaatt accatattgc cgtattgccg
     2041 ggggacggta ttggtccgga agtgatgacc caggcgctga aagtgctgga tgccgtgcgc
     2101 aaccgctttg cgatgcgcat caccaccagc cattacgatg taggcggcgc agccattgat
     2161 aaccacgggc aaccactgcc gcctgcgacg gttgaaggtt gtgagcaagc cgatgccgtg
     2221 ctgtttggct cggtaggcgg tccgaaatgg gaacatttac caccagacca gcaaccagaa
     2281 cgcggcgcgc tgttgccttt gcgtaagcac ttcaaattat tcagcaacct gcgtccggca
     2341 aaactgtatc aggggctgga agcattctgt ccgctgcgtg ctgacattgc cgctaacggc
     2401 ttcgacatcc tgtgcgtgcg cgaactgacc ggcggcatct atttcggtca gccaaaaggc
     2461 cgcgaaggta gcggacagta tgaaaaagcg tttgataccg aggtgtatca ccgttttgag
     2521 atcgaacgta tcgcccgcat cgcgtttgaa tctgcccgca agcgtcgcca caaagtcacc
     2581 tcaatcgaca aagccaacgt gctgcaatcc tctattttat ggcgggagat cgttaacgag
     2641 atcgccacgg aatacccgga tgtcgaactg gcgcatatgt acatcgacaa cgccaccatg
     2701 cagctgatta aagatccatc acagtttgac gtcctgctgt gctccaacct gtttggcgac
     2761 attctgtctg acgagtgcgc aatgatcact ggctcgatgg ggatgttgcc ttccgccagc
     2821 ctgaacgagc aaggttttgg tctgtatgaa ccggcaggcg gctcagcacc agatatcaca
     2881 ggcaaaaaca tcgccaaccc gattgcgcaa attctgtcgc tggcactgct gctgcgctac
     2941 agcctggatg ccgatgatgc ggcttgcgcc attgaacgcg ccattaaccg cgcattagaa
     3001 gaaggcattc gcaccgggga tttagcccgt ggcgctgccg ccgttagtac cgatgaaatg
     3061 ggcgatatca ttgcccgcta tgtggcagaa ggggtgtaat catggctaaa acgttatacg
     3121 aaaaattatt cgacgctcac gtagtgtacg aagccgaaaa cgaaaccccg ctgttatata
     3181 tcgaccgcca cctggtgcat gaagtgacct caccgcaggc gttcgatggt ctgcgcgccc
     3241 acggtcgccc ggtacgtcag ccgggcaaaa ccttcgccac tatggatcac aacgtctcta
     3301 cccagacgaa agacattaat gcctgtggtg aaatggcgcg catccagatg caggagctga
     3361 ttaaaaactg caaagaattt ggcgtcgaac tgtatgacct gaatcacccg tatcagggga
     3421 tcgtccacgt aatggggccg gaacagggcg tcaccttgcc ggggatgacc attgtctgcg
     3481 gcgactcgca taccgccacc cacggcgcgt ttggcgcact ggcctttggt atcggcactt
     3541 ccgaagttga acacgtactg gcaacgcaaa ccctgaaaca gggccgcgca aaaaccatga
     3601 aaattgaagt ccagggcaaa gccgcgccgg gcattaccgc aaaagatatc gtgctggcaa
     3661 ttatcggtaa aaccggtagc gcaggcggca ccgggcatgt ggtggagttt tgcggcgaag
     3721 caatccgtga tttaagcatg gaaggtcgta tgaccctgtg caatatggca atcgaaatgg
     3781 gcgcaaaagc cggtctggtt gcaccggacg aaaccacctt taactatgtc aaaggccgtt
     3841 tgcatgcgcc gaaaggcaaa gatttcgacg acgccgttgc ctactggaaa accctgcaaa
     3901 ccgacgaagg cgcaactttc gataccgttg tcactctgca agcagaagaa atttcaccgc
     3961 aggtcacctg gggcaccaat ccaggccagg tgatttccgt gaacgacaat attcccgatc
     4021 cggcttcgtt tgccgatccg gttgaacgcg cgtcggcaga aaaagcgctg gcctatatgg
     4081 ggctgaaacc gggcattccg ctgaccgaag tggctatcga caaagtgttt atcggttcct
     4141 gtaccaactc gcgcattgaa gatttacgcg cggcagcgga gatcgccaaa gggcgaaaag
     4201 tcgcgccagg cgtgcaggca ctggtggttc ccggctctgg ccaggtaaaa gcccaggcgg
     4261 aagcggaagg tctggataaa atctttattg aagccggttt tgaatggcgc ttgcctggct
     4321 gctcaatgtg tctggcgatg aacaacgacc gtctgaatcc gggcgaacgt tgtgcatcca
     4381 ccagcaaccg taactttgaa ggccgccagg ggcgcggcgg gcgcacgcat ctggtcagcc
     4441 cggcaatggc tgccgctgct gctgtgaccg gacatttcgc cgacattcgc aacattaaat
     4501 aaggagcaga ccatggcaga gaaatttatc aaacacacag gcctggtggt tccgctggat
     4561 gccgccaatg tcgataccga tgcaatcatc ccgaaacagt ttttgcagaa agtgacccgt
     4621 acgggttttg gcgcgcatct gtttaacgac tggcgttttc tcgatgaaaa aggccaacag
     4681 ccaaacccgg acttcgtgct gaacttcccg cagtatcagg gcgcttccat tttgctggca
     4741 cgagaaaact tcggctgtgg ctcctcgcgt gagcacgcgc cctgggcatt gaccgactac
     4801 ggttttaaag tggtgattgc gccgagtttt gctgacatct tctacggcaa tagctttaac
     4861 aaccagctgc tgccggtgaa attaagcgat gcagaagtgg acgaactgtt tgcgctggtg
     4921 aaagccaatc cggggatcca tttcgacgtg gatctggaag cgcaagaggt gaaagcggga
     4981 gagaaaacct atcgctttac catcgatgcc ttccgccgcc actgcatgat gaacggtctg
     5041 gacagtattg ggcttacctt gcagcacgac gacgccattg ccgcttatga agcaaaacaa
     5101 cctgcgttta tgaattaatc cccttgcccg gtcaaatgac cgggctttcc gctatcgtcc
     5161 acgtcatcaa acgtctttaa cctttgcggt taaaaataat gcgacaacag aaataaccgc
     5221 aattacccag tagacagcaa agtgccccca actttgtgca attgctccct gaataacgcc
     5281 agccagaatt accccggtag aaatactgtt agtaaataag gtggtagctg cccccgctct
     5341 tccaggcatt aaatcctgaa accatagcat cccaataccc gcaacaatgc cgataaatac
     5401 agcgttaaaa agttgcagcg tcatcaacgc catacggcta tgaaagaaaa tcaatccggt
     5461 gtaaaacagt actcctgccg ccactgctat gaccaccatt cgccgcttac cataacgttt
     5521 gacatagtag ccagccagaa tcattgctgg tatttccagt ccagctgccg tccccatcag
     5581 gaaacccgcc agtttgtctg gcaatcctaa ctcgctactg atccacaacg gcatatcaat
     5641 aatgtacatg gtgttgcagg tccacattaa cgtcgaggcg acaaataaca tccgtacgtt
     5701 actatcctgc cagccacctt gcattgataa agcattttcc gacggcagtt ctacccgcgc
     5761 cacagacgga agcataaatg caatcaatac cagactgagt gtgaatatcc cggcggcaat
     5821 cgaaaacatc accacttctc gcgccgagtt atccgcatat tcccgcgcca gagcaaataa
     5881 ctgtggcatt gccgtattgg ccagagatgc cagaagcaca ccacaggtga taagcgtcag
     5941 ataatgacga ttaaatgcaa acaatggcgc attgccgata gccatcaaac agcaaaatat
     6001 aatcagtttt cgccgatcgc cctgactgtc agaacgtttt gccaaccaga ggcttacgcc
     6061 gatcccagca atagtattca ccgtataaaa gagtccgatc cagaaaggtt gcgcgccaac
     6121 ctcacgactc agaaataagc tcaatgtagg agcctgtagc gccccggcca cccccatcat
     6181 aaaagcgacc agcataaatg ccgcgtaaac accgttcata cggcgagcca tcgtcattat
     6241 ccagatcata cgttcccttt ttagcgcggc gagaataaaa aaaaccagca ggtataatct
     6301 gctggcgggt gattttacac caatactcag tcacacatga tgcaggcaag tcaactttca
     6361 gaattgcggt catcccactg catcagtcct tcaagcatgg ttaatcgttg tgggacgctt
     6421 aaccaacgca accagcacaa cttcgctgtc gcggtaaaat agtgctgata aaactgacgc
     6481 atggggcacc tccttgcttc atcgttagga attattgact taatataggg aaaataaaat
     6541 tgctgtcttt tgcgcaggag ttcccctttt atgccatctg ctcgtctgca acaacagttc
     6601 atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca caacgctcaa cgaactggca
     6661 gcgttattga gctgctcgcg tcgtcatatg cgtaccctgc tcaacaccat gcaggatcgc
     6721 ggctggctga cgtgggaagc ggaagtcggg cgcggtaaac gctcgcgtct gacattcctc
     6781 tataccgggc tggcgcttca gcaacagcgg gcggaagacc tgctggagca ggatcgtatc
     6841 gaccaactag tgcagttggt tggcgacaaa gcgactgtgc ggcaaatgct ggtttctcat
     6901 ctgggccgca gcttccgcca ggggcggcac atcctgcgcg tgctctacta tcgtccgttg
     6961 cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa cccatattgc ccggcaaatc
     7021 ttcagttcgc taacgcgcat aaatgaggaa aatggggaac tggaagcaga catcgcccac
     7081 cactggcagc aaatatcacc gcttcactgg cgtttctttt tgcgtccagg agtccatttt
     7141 catcatggtc gtgaactgga aatggacgac gtgatcgcct ctttaaaacg aatcaatacg
     7201 ctgccgctct attcgcatat tgcggacatt gtgtcgccga cgccctggac gctggatatc
     7261 catctcacgc aaccggaccg ctggttaccg ttactgctgg ggcaagttcc ggcgatgatc
     7321 ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc atcccatcgg caccggtccg
     7381 tatgcggtga tccgcaacac caccaatcaa ctgaaaattc aggcattcga tgacttcttc
     7441 ggttaccggg cattaatcga tgaagttaac gtctgggttc tgccggaaat tgccgacgag
     7501 ccagctggag gtctgatgct aaaaggtcca cagggtgagg aaaaagagat tgaaagccgc
     7561 ctggaggaag gttgctacta tttactattc gacagccgca cccatcgcgg agcgaatcag
     7621 caagtcaggg actgggtaaa ctatgtgctt tctccaacta atctggtcta tttcgctgag
     7681 gaacagtacc agcaactgtg gttcccggct tatggactgc tcccccgttg gcaccatgcc
     7741 cgcaccataa agagcgaaaa accggctggc ctggaaagcc tcaccctgac cttttatcag
     7801 gatcacagtg agcatcgggt gattgccggg atcatgcagc agattctggc aagtcaccag
     7861 gtcacgctgg aaatcaaaga gatcagctac gatcagtggc atgaaggaga gatcgagagc
     7921 gatatctggc ttaatagcgc caactttacg ctgccgctgg atttttcgct gttcgcgcac
     7981 ttgtgcgagg tgccgctgct ccaacactgt attccgatcg actggcaagc cgacgccgcc
     8041 cgctggcgca atggcgaaat gaacctggcg aactggtgcc agcaactggt cgccagtaaa
     8101 gcaatggtgc cgcttatcca ccactggctg atcattcagg gacaacgcag tatgcgcggc
     8161 ctgcgtatga ataccctcgg ctggttcgat tttaaatcag cgtggtttgc gccgccggat
     8221 ccatgattgc tggtagataa caaaatcact acactaacgc cgttctcaac ggggtgccac
     8281 gcaaacgcgt gcgctgagaa aatacccgtc gaacctgatc cggataacgc cggcgaaggg
     8341 atttgaggct ccttctcaag tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa
     8401 atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc gctaaacccg ttctgactgt
     8461 ttatacctac gattccttcg ccgccgactg ggggcctggt ccaaagatca aaaaagcttt
     8521 tgaagccgac tgtaactgcg aactgaaact ggtggcgctg gaagatggcg tttcgcttct
     8581 caaccgtcta cggatggaag gcaaaaatag taaagccgat gtggtgctgg ggctggataa
     8641 caatctgtta gacgccgcca gtaaaaccgg actatttgcc aaaagcggtg tggcagcgga
     8701 tgccgttaac gttcccggcg gctggaataa tgacactttc gtcccgtttg attacggcta
     8761 tttcgccttc gtctatgaca agaacaaact gaaaaacccg ccgcaaagcc tgaaagaatt
     8821 ggttgagagc gatcaaaact ggcgggtgat ttatcaggat ccgcgcacca gtacgccggg
     8881 gctgggcctg ctgttgtgga tgcaaaaagt ctatggcgat gacgccccac aagcctggca
     8941 aaaactggcg aagaaaaccg tcacggtcac caaaggctgg agcgaagcct acggcctgtt
     9001 tttaaaaggt gaaagcgatc tggtactgag ttacaccacc tctccggctt atcacattct
     9061 cgaagagaaa aaagataact acgccgccgc gaactttagc gaaggtcact atctgcaggt
     9121 ggaagtcgcc gcccgcaccg ctgccagcaa gcagccggag ctggcgcaaa aattcctcca
     9181 gtttatggtt tctccggctt ttcagaatgc gatcccaacc ggtaactgga tgtatccggt
     9241 ggcaaatgtc acgctgcctg ccgggtttga acaattgacc aaaccggcca ccacgctgga
     9301 gttcacacca gctgaagtgg cggcacaacg tcaggcatgg attagcggat ggcaacgcgc
     9361 cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa gcgccgccac gctggtggta
     9421 gcggttgcgc tcgcggcgtt tctcgccctg tggtggaacg cgccgcaggg taactgggtg
     9481 gcagtctggc aggacagcta cctgtggcat gtggtgcgct tctccttctg gcaggcgttt
     9541 ctctcggcgc tactctctgt cgtacccgcg atattcctcg cccgcgccct ctatcgcagg
     9601 cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa tgaccttgat cctcccggtg
     9661 ctggttgctg ttttcggcat tcttagcgtc tatggtcgcc agggctggct ggcatcgctc
     9721 tgccaatcgc tcggtctgga gtggaccttt tcgccctacg gcctgcaagg tattttgctg
     9781 gcgcacgtat tttttaatct gccgatggcg agccgcttat tactccaggc actggaaaac
     9841 attcccgacg aacaacgtca gcttgccgcc cagcttggga tgcgcggctg gcattttttc
     9901 cgcttcgtcg aatggccgtg gttacggcga caaatcccgc cggttgctgc acttatcttt
     9961 atgctctgtt tcgccagctt cgccaccgtg ctatcactgg gcggcggtcc gcaggcgacc
    10021 actatcgagc tggctattta tcaggcgctg agttacgact acgatcctgc ccgcgcggcg
    10081 atgctggcgc tgatccagat ggtgtgttgc cttgggctgg tactgttgag tcagcgattg
    10141 agtaaggcca ttgcgccagg caccacgctg ctgcaaggct ggcgcgaccc ggacgatcgt
    10201 ctgcatagcc gcatttgcga cacggtgtta atagtgctgg cgttgctgct gttgttgcca
    10261 ccgttgctgg cggtgatcgt cgatggggta aatcgccagt tgccggaagt gctggcacaa
    10321 ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg cgctggcggc aggtgtattg
    10381 tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg aacttcgggc acggcagaaa
    10441 atgctggcgg gtcaggcgct ggagatgagc ggcatgttga ttctcgccat gccggggatt
    10501 gtgctggcta ccggcttctt tttactgctc aacaacacta tcggcctgca acaatctgct
    10561 gacggcattg tgattttcac caatgcgtta atggcgatcc cttatgcgct gaaagtactg
    10621 gaaaacccga tgcgcgatat caccgcccgc tacagtatgt tgtgtcagtc gctgggcatt
    10681 gaaggctggt cgcgcttaaa agtggtggag ctgcgcgccc tgaaacgtcc actggcgcag
    10741 gcgctggcct ttgcctgcgt gctgtcgatt ggtgattttg gcgtggtggc gttgttcggt
    10801 aacgatgatt tccgtaccct gccgttttat ctctaccagc aaattggctc ctatcgcagc
    10861 caggacggcg cggtcaccgc gttaattctg ctgctactct gttttctgct gtttaccgtg
    10921 attgaaaaac taccggggcg aaatgttaaa actgactgat atcacctggc tttaccaaca
    10981 tttgccgatg cgttttagct taacggtgga acgcggcgag caggtggcga tcctcgggcc
    11041 aagcggcgca ggtaaaagta ccctgctgaa tttgattgcc ggttttctga cgccagccag
    11101 cggttcgctg actatcgatg gcgttgatca caccacgacg ccgccgtcac gccgtccggt
    11161 gtcgatgctg tttcaggaga acaacctgtt cagccacctg acggtcgcgc agaacatcgg
    11221 gctggggctt aatccaggat tgaagctgaa cgcggcacag caggagaaaa tgcacgctat
    11281 cgcccgtcag atggggattg ataatttaat ggcgcggtta ccgggcgagc tttccggcgg
    11341 ccagcgacag cgcgtggcgt tagcgcgttg tctggtgcgc gaacagccga ttttattgct
    11401 cgatgaaccg ttctctgcgc tcgatccggc gttacgtcag gagatgctga cgctggtgag
    11461 cacgagctgc cagcagcaaa aaatgacgct attgatggtg tcgcacagtg tggaagatgc
    11521 ggcgcggatc gccacacgct cggtggtggt cgccgacggg cgcatcgcct ggcagggtaa
    11581 gactgaagaa ttgttgagcg gtaaggcgag tgcttcggca atattgggga ttacgggtta
    11641 gtatgcactg tcagatgcgg cgtgaacacc ttatccgacc tacggacagt gcgttttgta
    11701 ggcctgataa gaccggtcag cgtcgcatca ggcaacccgc acaagacaat cccctaaacc
    11761 ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg aattaacacc
    11821 accagcgcca ccacgccgat ggcagaaatc aacggcgtca gccacaacaa acggccgcgc
    11881 gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg ccagcacagc
    11941 cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa ctcgccgctc
    12001 tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac
    12061 ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac cggcagatcc
    12121 agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg accgaccaga
    12181 atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa tgctttgttt
    12241 ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag ccagaaggag
    12301 atccagtcgc ccagcagaca accgacaatc cctgccagcc aggcgtgcca gaaacttaac
    12361 tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg tagaatcaaa
    12421 ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa tgaatacacg
    12481 gtagattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc aaatggacga
    12541 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
    12601 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
    12661 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
    12721 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
    12781 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
    12841 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
    12901 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat
    12961 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
    13021 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
    13081 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc
    13141 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
    13201 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg
    13261 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc
    13321 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
    13381 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
    13441 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
    13501 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
    13561 ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
    13621 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
    13681 ccacattgat tatttgcacg gcgtcacact ttgctattcc atagcatttt tatccataag
    13741 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
    13801 tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc agtgattctg
    13861 tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc gtagagtggt
    13921 atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc cgtcatcatc
    13981 cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca gagcttagcg
    14041 tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac
    14101 cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa aacccaaacg
    14161 cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt
    14221 tgtgccacgc gccgggcaac gttgactact cccgctacat tggtggtatt tattccagcg
    14281 aatggttctg ggcaaaaatc ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg
    14341 ccgcatcgtg gattgagctg tgcgactggg tgccagctct gctttccggt accacccgcc
    14401 cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg cacgaaagct
    14461 ggggcggctt gccgccagcc agtttctttg atgagctgga cccgatcctc aatcgccatt
    14521 tgccttcccc gctgttcact gacacctgga ctgccgatat tccggtgggc accttatgcc
    14581 cggaatgggc gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg
    14641 actgccatat gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta aaagttatcg
    14701 gtacttccac ctgcgacatt ctgattgccg acaaacagag cgttggcgag cgggcagtta
    14761 aaggtatttg cggtcaggtt gatggcagcg tggtacctgg atttatcggt ctggaagcag
    14821 gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc tggccgctgg
    14881 aacagcttgc cgcccagcat ccggaactga aagcgcaaat caacgccagc cagaaacaac
    14941 tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac ctgccggtgg
    15001 tgctcaactg gtttaacggc cgccgcacac cgaacgctaa ccaacgcctg aaaggggtga
    15061 ttaccgatct taacctcgct accgacgctc cgctgctgtt tggcggtttg attgctgcca
    15121 ccgcctttgg cgcacgcgca atcatggagt gctttaccga tcaggggatc gccgtcaata
    15181 acgtgatggc gctgggcggc atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg
    15241 acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg
    15301 cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa
    15361 tggccagtgc ggtagagaaa accctgcaac cgcgcagcga acagacacaa cgctttgaac
    15421 agctttatcg ccgctatcag caatgggcga tgagcgccga acaacactat cttccaactt
    15481 ccgccccggc acaggctgcc caggccgttc cgactctata aggacacgat aatgacgatt
    15541 tttgataatt atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg cccggaaacc
    15601 ctgcgtcagg tcacccaaca tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa
    15661 ctgccctgca aactggtgtt gaaaccgctg ggcaccacgc cggatgaaat caccgctatt
    15721 tgccgcgacg cgaattacga cgatcgttgc gctggtctgg tggtgtggct gcacaccttc
    15781 tccccggcca aaatgtggat caacggcctg accatgctca acaaaccgtt gctgcaattc
    15841 cacacccagt ttaacgcggc gctgccgtgg gacagtatcg atatggactt tatgaacctg
    15901 aaccagactg cacatggcgg tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa
    15961 catgccgtgg ttaccggtca ctggcaggat aaacaagccc atgagcgtat cggctcctgg
    16021 atgcgtcagg cggtctctaa acaggatacc cgtcatctga aagtctgccg ttttggcgat
    16081 aacatgcgtg aagtagcggt caccgatggt gataaagttg ccgcacagat caagttcggt
    16141 ttctccgtca atacctgggc ggttggcgat ctggtgcagg tggtgaactc catcagcgac
    16201 ggcgatgtta acgcgctggt cgatgagtac gaaagctgct acaccatgac gcctgccaca
    16261 caaatccacg gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga gctggggatg
    16321 aagcgtttcc tggaacaagg tggcttccac gcgttcacca ccacctttga agatttgcac
    16381 ggtctgaaac agcttccagg tctggccgta cagcgtctga tgcagcaggg ttacggcttt
    16441 gcgggcgaag gcgactggaa aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc
    16501 ggtctgcagg gcggcacctc ctttatggag gactacacct atcacttcga gaaaggtaat
    16561 gacctggtgc tcggctccca tatgctggaa gtctgcccgt cgattgccgt agaagagaaa
    16621 ccgatcctcg acgttcagca tctcggtatt ggtggtaagg acgatcctgc ccgactgatc
    16681 ttcaataccc aaaccggtcc agcgattgtc gccagcctga ttgatctcgg cgatcgttac
    16741 cgtctgctgg ttaactgtat cgacacggtg aaaacaccgc actccctgcc gaaactgccg
    16801 gtggcgaatg cgctgtggaa agcgcaaccg gatctgccaa ctgcttccga agcgtggatc
    16861 ctcgctggtg gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa cgatatgcgt
    16921 cagttcgccg agatgcacga cattgaaatc acggtgattg ataacgacac ccgcctgcca
    16981 gcgtttaaag acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc
    17041 acatcaggta tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct acacgctgcg
    17101 attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg
    17161 cgtcttatca ggcctacgcg ctgcgatttt gtagaccgga taagcgaagc gcatccggca
    17221 cgaaggagtc aacatgttag aagatctcaa acgccaggta ttagaagcca acctggcgct
    17281 gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg
    17341 cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat
    17401 ggtcgtggtt agcatcgaaa caggtgaagt ggttgaaggt acgaaaaagc cctcctccga
    17461 cacgccaact caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac
    17521 gcactcgcgc cacgccacca tctgggcgca gtcgggtcag tcgattccag caaccggcac
    17581 cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga
    17641 aatcaacggc gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca
    17701 gggtatcgat gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg
    17761 gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat
    17821 ggagatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga
    17881 taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa
    17941 aaccacagcc aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc
    18001 agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca
    18061 gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa
    18121 gagtccgtgg cgtttattcc cgccgatcag gttccccgcg ctcagcatat tttgcagggt
    18181 gaacaaggct ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat
    18241 ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt
    18301 ggcgttaccg tctacgaggc cgatgtgcgc ccgccagaac gctatctgat ggagcggttt
    18361 atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt
    18421 ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc
    18481 acccgccacg gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg catcgtttat
    18541 atgctggggt cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc
    18601 agccgcccgc agttgctgga aaaactcaac gcctggtttg ccaactacga tcctgatgtg
    18661 atcatcggtt ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt
    18721 taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc
    18781 tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag
    18841 gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag
    18901 ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc
    18961 cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg
    19021 acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgggc aacggtgaac
    19081 ggcctgccgg tggaccgaca cggcggttcg gtggcggcat ttggtcatct ctattttccg
    19141 cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc gcacgccagc
    19201 cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac
    19261 tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtg
    19321 gaaggcatgg cgcagcctga tccagagcac agtaccgaag gttttctcga tgcctggttc
    19381 tcgcgagaaa aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa
    19441 gccaaacgcc agggtaacaa accgctgtcg caggcgctga aaatcatcat gaatgccttt
    19501 tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc atcgtcgatc
    19561 accatgcgtg gtcatcagat catgcggcaa accaaagcgt tgattgaagc acagggctac
    19621 gacgttatct acggcgatac cgactcaacg tttgtctggc tgaaaggcgc acattcggaa
    19681 gaagaagcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa
    19741 acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac ccatttctgc
    19801 cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg ctacgccggg
    19861 ctgattcagg agggcgataa acagcggatg gtgtttaaag ggctggaaac cgtgcgcacc
    19921 gactggacgc cgctggccca gcagtttcag caggagctat acctgcgcat cttccgcaac
    19981 gagccatatc aggaatatgt acgcgaaacc atcgacaaac tgatggcggg tgaactggat
    20041 gcgcgactgg tttaccgtaa acgccttcgc cgtccgctga gcgagtatca gcgtaatgtg
    20101 ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg tggtcgcccc
    20161 ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg cccggagccg
    20221 ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca gctacaaccc
    20281 gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat gaccgggcaa
    20341 cttgggctat tttgagcaaa aaaagagttc gccagatacc attttgatgc gtgacgaatg
    20401 ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac accactacct
    20461 cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc gaacacatat
    20521 gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg gacttggaac
    20581 cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg gtgaaaaccg
    20641 tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat
    20701 taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa atggcttgct
    20761 gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc gtgaagtttt
    20821 ccttgatagc aaactggtgt tcagcaaacc gcaggaccgc ctgtttgccg ggcagattga
    20881 ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg aacagttccg
    20941 tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcacc agctcaacat
    21001 cgctcatgat gttggccgcc gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt
    21061 agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg gcgctgctga
    21121 acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag aaatgctgcg
    21181 ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag ctcagcacga
    21241 tgcttacaac ccgttcgaca ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg
    21301 tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg tggtcgatga
    21361 agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg ccattgaaca
    21421 actggcagag cacatgccgg gcgttctgct gctgaccgcg accccggaac agctggggat
    21481 ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg attttgcgca
    21541 gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca tgctgctggc
    21601 aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg gcgagcagga
    21661 tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca
    21721 ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac
    21781 gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc tgccgctacc
    21841 gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga
    21901 agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg aaggtgataa
    21961 cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc tgaccagcca
    22021 tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc aactggagca
    22081 ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat
    22141 cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac aggtactgct
    22201 gtgctcagaa atcggttctg aaggacgtaa cttccagttc gccagccaca tggtgatgtt
    22261 tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg
    22321 ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc agtcggtgct
    22381 ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga ccggacgcac
    22441 tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg atcaaaccga
    22501 aggctttgac gatctgatca aaaactgccg cgagcaacat gaagcgctga aagcacagct
    22561 ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa aagcccaggc
    22621 actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct tcgccatgaa
    22681 cctgctcgat attatcggta tcaatcagga cgatcgcggc gacaacatga tcgtgctgac
    22741 gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg gcatcaccat
    22801 cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta cctgggagca
    22861 tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta gcagcacgat
    22921 ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac tgatttatgt
    22981 ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac cgacgccggt
    23041 acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt ttgaaacctt
    23101 taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta acgccgtgca
    23161 gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc
    23221 attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc tgtctcgtct
    23281 ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg ccattgagag
    23341 caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg
    23401 tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca
    23461 ccgcaggaac cctggttggt tatcctgtat caggatgacc acattatggt ggttaacaag
    23521 ccgagcggtt tgttgtctgt gccggggcgt ctggaagagc acaaagacag cgtgatgacg
    23581 cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat ggctaccagc
    23641 ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg ccagttccgc
    23701 gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc ccctgcggaa
    23761 ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt
    23821 tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc ggcggataac
    23881 acggcaagag tggtgttaaa accgattact gggcgttcgc atcaattgcg tgtgcatatg
    23941 ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga agcgagagcg
    24001 atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc ggcgtatggc
    24061 aatagtatga cgtttaaagc gccagcggat ttttaatagt cacgctggcg cagttcggta
    24121 ggcggataag gcgttcacgc cgaatccggc aattgtgcgc cgattgcctg atgcgacgcg
    24181 ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg ctgctttatc
    24241 agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat catctcaggc
    24301 ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt acggtaggct
    24361 cgtttaatgg tggtcgcatc atccgtcggc ttcacaccca gcacattaca ggcatcttcc
    24421 agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg ctgataaccg
    24481 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
    24541 atccctaatt cttctgcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
    24601 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
    24661 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc tgattttccc
    24721 acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat tcggtccatc
    24781 agctggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
    24841 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac
    24901 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
    24961 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
    25021 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
    25081 gtgcttttac cgttttccgg ctgcagtcaa ttgtgggaca tcgcctataa tttgcattat
    25141 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
    25201 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
    25261 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
    25321 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
    25381 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
    25441 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
    25501 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
    25561 ggcaccagga caaccggagc cggtacgaac cgttgatgcg ctcggtaatg tccattacga
    25621 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
    25681 caacgtctgg gaaggtgatt accagatggt gggtcgccag ggccgcggta aagcggacct
    25741 gatgaaacaa cgtggtgaaa accgctatac cattctggat aacggtagct ttacctcctg
    25801 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
    25861 acaagttgcg gagatctgga acgcccgctt taaggtgggt ccagtaccga tcttttatag
    25921 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
    25981 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
    26041 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
    26101 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
    26161 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttgtt
    26221 ctactggcaa cattccgggg tcatggatca agtgtggcgt ttcaacgtag actacaccaa
    26281 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
    26341 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
    26401 aaccaagcag ttccaggtct ttagcgagca gaacaccagt agctactcgg cagagccgca
    26461 gttagacgtt aactactacc agaatgatgt tggtccgttt gatacgcgta tttacggcca
    26521 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
    26581 accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg aagcgaagtt
    26641 gctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa acacgaccaa
    26701 gctggacgaa tccgttaacc gcgttatgcc gcaattcaaa gttgacggca aaatggtctt
    26761 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
    26821 gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact cgtctctact
    26881 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
    26941 ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
    27001 acattttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
    27061 caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag gcgatactta
    27121 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
    27181 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    27241 gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta agtactattc
    27301 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gctggccaat
    27361 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    27421 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    27481 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    27541 ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc aagagatgct
    27601 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    27661 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    27721 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    27781 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    27841 tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc caccaaatca
    27901 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    27961 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    28021 tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacacc tatcgtaacc
    28081 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    28141 ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    28201 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acttctgatc
    28261 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    28321 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    28381 tgggctgggg ccgtattcag gagttgccgg gtatcttcgc ccaggcatta agcaccgcga
    28441 agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    28501 acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    28561 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg tgtgaaactg gaacagattg
    28621 ctgccgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctctcagg
    28681 atccaggctc tgctaaccag ggcggtgatc tcggctgggc tacaccagat attttcgatc
    28741 cggccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca ccggttcact
    28801 cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat aaaaccgacg
    28861 ctgcgcagaa ggatcgtgca taccgcatgc tgatgaaccg taagttctcg gaagaagcag
    28921 caagctggat gcaggaacaa cgcgccagcg cctacgttaa aatcctgagc aactaatggt
    28981 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    29041 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    29101 ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt attcccccaa
    29161 ctcccctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg cgctacgcga
    29221 atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg agacgctggc
    29281 gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    29341 taaaggcgtc attaacgacg caggtattcc gtttactggt cataccgagt ttttcgaaga
    29401 gcgctcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    29461 ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg cacttttgca
    29521 cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtattg ccgaaccgcg
    29581 cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga
    29641 gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga tactcaacgg
    29701 gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg acgccgtgct
    29761 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    29821 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    29881 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    29941 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    30001 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    30061 aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc attgaccgag
    30121 ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga tctggcggca
    30181 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    30241 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    30301 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    30361 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    30421 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt gatcccggta
    30481 ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    30541 ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgtgtgtt gagccgcatc
    30601 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    30661 agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga aaatatctct
    30721 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    30781 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggcac
    30841 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    30901 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    30961 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    31021 agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg cagggtcact
    31081 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    31141 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    31201 gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct gggaaagata
    31261 ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct
    31321 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    31381 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    31441 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctttg ctgcaaatcg
    31501 acgaagagaa aaagttggtg atggcccacg cagggatcac gccgcagtgg gatctacaga
    31561 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctatc gagtgactcc tatcccttct
    31621 ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    31681 gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc ccgaacggtc
    31741 aactggatat gtacagcaaa gaatcgccgg aagaggcccc tgccccactg aaaccgtggt
    31801 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    31861 tggagggcaa aggtacgccg gaaggtatat acgcgctgga taccggctgc tgctggggtg
    31921 gtacattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg tcgaaccggc
    31981 ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatatagga aggccggata
    32041 agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaaat taccgccgct
    32101 ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga
    32161 ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg
    32221 cgtcgatatg cgtcagatac agtttctgcg cttttggcag gaactgctca taaacgcgac
    32281 cgccgccaat caccatgatt tctggtacgt caccacacgc cgcgatggct tcatccaccg
    32341 acttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc
    32401 gtcctggcaa cggacgaccg attgattccc aggtatggcg gcccataatc acgggtttat
    32461 ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt
    32521 ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt gagatttccc
    32581 gataaaaaaa attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga
    32641 tgatgttgag gaaaatttta tattctgctg gcgagtccac gctctctccc tggactcgcc
    32701 gcattacaat gaaacaaaaa caaacagtta gctgtaaagt gtgatttatg tcactcttta
    32761 ttaggatgag ggtttcgttt ccggttcatc cgccatgttg ccggtatgtt taccttcttc
    32821 ggttccctgc cagccatgtc gttgaattaa tgacagatgt tcgcggtcct cggtaatgat
    32881 ctcacttaac atcgcgctgg tgcgtttata gaccgccgcg cgggctttgg tgtcgttctc
    32941 aaccattgcc atctcttcca ccatctgaat attaaagcgg cggaacacat cggcacgttc
    33001 tcgcgcttca tacggcccca gacctaaact ttccagtgcc agacgcccgg ttttcagcgc
    33061 accttcgaag gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg
    33121 gtcgacatcg cgggcgcggg caataatctg caaatgcggg aagtgttctt tcaccatctc
    33181 tgtcagttgc aggttggttt gcggatcgtc gatggcgtta atcagcactt ccgctttcgc
    33241 cgctcccgca gattccagta aatccatccg cgtggcatcg ccataaaaca ctttcatacc
    33301 aaatttacgc aaggtttcga tatggtccgg atcgtgatcg agcaccacca ttttcacccc
    33361 gctggagagc agtaaacgtc cggtgatctg cccaaaacga ccgaatccgg caatgattac
    33421 ccgcggctgt tcttcgtcga tctcatcggc ttcacgcgcc tcttcagtag aagattgctc
    33481 aaggcgattg aggatcacca gcagaatcgg cgttgccgcc atcgacagcg ccaccgccag
    33541 ggtcagcgat ttcgcccact ccggctccag cacattcgcc atctgcgccg cgccaaatac
    33601 cacaaaggca aactcactgc cctgccctaa caacaccgca aaccaacgac gctgtttatt
    33661 tggcacttgc aacggtcggg caatcagcca cagcatggcg attttgatga tgaggaaacc
    33721 gagcagcaaa atgacaatgc gcaatgggtt ttcaagcagc gtgccaaagt ctatcgacat
    33781 gccaacaccg atgaaaaaca gccccaacag caaaccttta aagggttcga tatcgctctc
    33841 cagcgcatga cggtattccg agcttgccag cagtacgccc gccagaaacg cgcccatcgc
    33901 catcgacaag ccgacctctt ccagcagcaa accaaagcca aacacgagga ataacgccac
    33961 ggcactaaac acttcccgca agccagagcg ggctacaaaa cgcagcgccg gacgcgtgac
    34021 atagcgcccc agcaatacca ccagcaccag cgcacccgcc acttttaacg ccgagagagc
    34081 aaatgcgccc atcgtcgtcg aggcactgct cgttgccagt agcggaatca tcgccaccag
    34141 cgggatcgcc gcgatatcct ggaacagcag caccgcaaag gcactgcgac ccatttgcgt
    34201 caccatcaga ttgcgttcat tcatcgcctg catggcaatc gccgtagagg agagcgccag
    34261 cgtcatgccg atcaattccg cgacctgcca gcgcagccca agtaacatgc agaacagccc
    34321 cagcaggccg ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag
    34381 cttccacagc ctttgtggat cgagttcgag gccgataata aacagcatca gcaccacccc
    34441 aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc cccacgggcc
    34501 aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac gtaccgcaat
    34561 gggtacaatc agcgctgccg aaccgagata aatcagcgcc tgaatcagcg tatggctatc
    34621 catgatgggc ctcctgccat tccagcagac gttgcttata gtgacgcgcc tgcccttcga
    34681 gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc
    34741 cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc ggatgcgcac
    34801 caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa tgtttgccat
    34861 gcagcgcctt gccgccgtga ccgtaagccc agccgtgcga caaaacttta tcgatccaaa
    34921 gtttaagaag cggaggaatg ctgtaccact gcatcggatg ctgccagacg atcagatcgg
    34981 cgcgagacag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga tagagttgat
    35041 aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc cgtttattcg
    35101 catgggaatg atgcggatac ggatgcgcat aaattataag aatcataggt agcccgcgtg
    35161 attacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta tagtcaataa
    35221 tattgattca gaaaagagag aaatctgaac aatttacccc attctctgcc cgcggatcgt
    35281 catattgctg gtttgcgcca atgacatccc tcgatgtctc cggggcaaac gctacggaaa
    35341 tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg ataccgtaat
    35401 tattgataaa gatcggcagt gcccaggtcg aaacaatggt gccaatacgg cttaaggaca
    35461 taatcacgcc cacggcagag gcgcggatat ctgtcgggaa gagttcatta ggatagagcc
    35521 actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc atcactacca
    35581 gccagatccc catatccggg atcagcccca aaaccgccag cgccagcgtc atcatggcaa
    35641 agctgccaat caacaatgga cgccgtccgg cagtgtttaa ccacagcatc ggcggaatac
    35701 agccgagcat aaagaacagg ctaatcacca cgttccccag cgccgcgttt ttgccaacac
    35761 ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac attgggatca
    35821 cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca
    35881 gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt tcgccaaaca
    35941 gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac cagcggggag
    36001 attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgcc gcgctaccca
    36061 gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccaga tcggcgcagg
    36121 tcgcgccgac ataccacatg gcggcgataa agctgatgga aaatgcccgc tgacgggtac
    36181 tggagaactc ggtgatcatt gaggtggcga tgggataatc ggcaccgatg acaatgccga
    36241 taagtacccg catcaccaac agttcgacgg gggatgaaac aaacatcgtc gccaccgata
    36301 tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact ttatcggaaa
    36361 tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccaacaagc
    36421 caatccagtc agcgtccagt tttagcgccg gagtcagttg ctccagcgcc acgccaatca
    36481 ttaccagtac ataaccatcc agaa
//
LOCUS       DAJVTY010000019        36434 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVTY010000019 DAJVTY010000000
VERSION     DAJVTY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36434
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.019"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            22..918
                     /locus_tag="OQQ66_000884"
     CDS             22..918
                     /locus_tag="OQQ66_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1903900.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(915..2393)
                     /gene="scpC"
                     /locus_tag="OQQ66_000885"
     CDS             complement(915..2393)
                     /gene="scpC"
                     /locus_tag="OQQ66_000885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1903901.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETDKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2417..3202)
                     /gene="scpB"
                     /locus_tag="OQQ66_000886"
     CDS             complement(2417..3202)
                     /gene="scpB"
                     /locus_tag="OQQ66_000886"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1903902.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3213..4208)
                     /gene="meaB"
                     /locus_tag="OQQ66_000887"
     CDS             complement(3213..4208)
                     /gene="meaB"
                     /locus_tag="OQQ66_000887"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1903903.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4201..6345)
                     /gene="scpA"
                     /locus_tag="OQQ66_000888"
                     /pseudo
     CDS             complement(4201..6345)
                     /gene="scpA"
                     /locus_tag="OQQ66_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6549..7442)
                     /gene="argP"
                     /locus_tag="OQQ66_000889"
     CDS             complement(6549..7442)
                     /gene="argP"
                     /locus_tag="OQQ66_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1903904.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7825..8484
                     /gene="rpiA"
                     /locus_tag="OQQ66_000890"
     CDS             7825..8484
                     /gene="rpiA"
                     /locus_tag="OQQ66_000890"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1903905.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8739..9971
                     /gene="serA"
                     /locus_tag="OQQ66_000891"
     CDS             8739..9971
                     /gene="serA"
                     /locus_tag="OQQ66_000891"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1903906.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10200..10259
                     /gene="ibsC"
                     /locus_tag="OQQ66_000892"
     CDS             10200..10259
                     /gene="ibsC"
                     /locus_tag="OQQ66_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1903907.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10592..10651
                     /locus_tag="OQQ66_000893"
     CDS             10592..10651
                     /locus_tag="OQQ66_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1903908.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10752..11354)
                     /locus_tag="OQQ66_000894"
     CDS             complement(10752..11354)
                     /locus_tag="OQQ66_000894"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1903909.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11375..11558)
                     /gene="ssrS"
                     /locus_tag="OQQ66_000895"
     ncRNA           complement(11375..11558)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQQ66_000895"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11600..11929)
                     /gene="zapA"
                     /locus_tag="OQQ66_000896"
     CDS             complement(11600..11929)
                     /gene="zapA"
                     /locus_tag="OQQ66_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1903910.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12097..12675
                     /gene="ygfB"
                     /locus_tag="OQQ66_000897"
     CDS             12097..12675
                     /gene="ygfB"
                     /locus_tag="OQQ66_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1903911.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12701..14026
                     /gene="pepP"
                     /locus_tag="OQQ66_000898"
     CDS             12701..14026
                     /gene="pepP"
                     /locus_tag="OQQ66_000898"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1903912.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHSARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14023..15201
                     /gene="ubiH"
                     /locus_tag="OQQ66_000899"
                     /gene_synonym="visB"
     CDS             14023..15201
                     /gene="ubiH"
                     /locus_tag="OQQ66_000899"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1903913.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15224..16426
                     /gene="ubiI"
                     /locus_tag="OQQ66_000900"
                     /gene_synonym="visC"
     CDS             15224..16426
                     /gene="ubiI"
                     /locus_tag="OQQ66_000900"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1903914.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16500..16583)
                     /locus_tag="OQQ66_000901"
     CDS             complement(16500..16583)
                     /locus_tag="OQQ66_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903915.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16873..17967
                     /gene="gcvT"
                     /locus_tag="OQQ66_000902"
     CDS             16873..17967
                     /gene="gcvT"
                     /locus_tag="OQQ66_000902"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1903916.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17991..18380
                     /gene="gcvH"
                     /locus_tag="OQQ66_000903"
     CDS             17991..18380
                     /gene="gcvH"
                     /locus_tag="OQQ66_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1903917.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18499..21372
                     /gene="gcvP"
                     /locus_tag="OQQ66_000904"
     CDS             18499..21372
                     /gene="gcvP"
                     /locus_tag="OQQ66_000904"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1903918.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSHEVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21638..22381
                     /locus_tag="OQQ66_000905"
                     /pseudo
     CDS             21638..22381
                     /locus_tag="OQQ66_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22438..23871)
                     /gene="bglA"
                     /locus_tag="OQQ66_000906"
     CDS             complement(22438..23871)
                     /gene="bglA"
                     /locus_tag="OQQ66_000906"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1903919.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWCWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23916..24227
                     /locus_tag="OQQ66_000907"
     CDS             23916..24227
                     /locus_tag="OQQ66_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS1903920.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24391..25050
                     /locus_tag="OQQ66_000908"
     CDS             24391..25050
                     /locus_tag="OQQ66_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1903921.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25127..26107)
                     /gene="ygfZ"
                     /locus_tag="OQQ66_000909"
     CDS             complement(25127..26107)
                     /gene="ygfZ"
                     /locus_tag="OQQ66_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1903922.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26350..26616
                     /gene="sdhE"
                     /locus_tag="OQQ66_000910"
     CDS             26350..26616
                     /gene="sdhE"
                     /locus_tag="OQQ66_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1903923.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26597..27004
                     /locus_tag="OQQ66_000911"
     CDS             26597..27004
                     /locus_tag="OQQ66_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS1903924.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27044..27565)
                     /gene="fldB"
                     /locus_tag="OQQ66_000912"
     CDS             complement(27044..27565)
                     /gene="fldB"
                     /locus_tag="OQQ66_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1903925.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27677..28573
                     /gene="xerD"
                     /locus_tag="OQQ66_000913"
     CDS             27677..28573
                     /gene="xerD"
                     /locus_tag="OQQ66_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1903926.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28598..29308
                     /gene="dsbC"
                     /locus_tag="OQQ66_000914"
     CDS             28598..29308
                     /gene="dsbC"
                     /locus_tag="OQQ66_000914"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1903927.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29314..31047
                     /gene="recJ"
                     /locus_tag="OQQ66_000915"
     CDS             29314..31047
                     /gene="recJ"
                     /locus_tag="OQQ66_000915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1903928.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31138..32236
                     /gene="prfB"
                     /locus_tag="OQQ66_000916"
     CDS             join(31138..31212,31214..32236)
                     /gene="prfB"
                     /locus_tag="OQQ66_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1903929.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32246..33763
                     /gene="lysS"
                     /locus_tag="OQQ66_000917"
     CDS             32246..33763
                     /gene="lysS"
                     /locus_tag="OQQ66_000917"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1903930.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITK
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33806..34354)
                     /gene="idi"
                     /locus_tag="OQQ66_000918"
     CDS             complement(33806..34354)
                     /gene="idi"
                     /locus_tag="OQQ66_000918"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1903931.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34409..34480
                     /locus_tag="OQQ66_000919"
     CDS             34409..34480
                     /locus_tag="OQQ66_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903932.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34477..34602)
                     /locus_tag="OQQ66_000920"
     CDS             complement(34477..34602)
                     /locus_tag="OQQ66_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903933.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34604..36052)
                     /gene="uacT"
                     /locus_tag="OQQ66_000921"
     CDS             complement(34604..36052)
                     /gene="uacT"
                     /locus_tag="OQQ66_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1903934.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8780 a         9167 c         9741 g         8746 t
ORIGIN      
        1 gtacgaataa agtaacgggg gatggacatt tttatttcga aaaagatgcg caatttcatt
       61 ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat gacggcttcg
      121 ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct atttacccgc
      181 aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa actgttcccg
      241 gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg tcgttcacga
      301 gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca gttgattagt
      361 ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga aaacggcgtt
      421 atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca tgtatctgca
      481 cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg attgggcttt
      541 ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga gctgccctgg
      601 ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc gaatatgcga
      661 ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc caaaatcagc
      721 gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg actggtgaat
      781 ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca ggaatatctt
      841 tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc tgaagatcgt
      901 gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg gtgtaagtcg
      961 aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag atagcgatgc
     1021 agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc gcgttgaagc
     1081 ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt gacgctgtgt
     1141 tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc ttctttagca
     1201 atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc gctgccgccg
     1261 atgccgttca tcagatcgac cccggctacg tgcgttgaat ttgcatgccc gtaaatatca
     1321 aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc cgggttattg
     1381 gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt gtcgtaaatc
     1441 ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct gattttgtcg
     1501 gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat gaacggagga
     1561 atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc cacgccactt
     1621 tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa taagaacgtg
     1681 accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat attaccggcg
     1741 tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg cacatagcgg
     1801 gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg tggcgcgcca
     1861 ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt aagttcaatg
     1921 atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct ggttaaccag
     1981 actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat gtcgccgaag
     2041 aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa actcaccgcg
     2101 ccctgattga tctttttacg taaaccggac gatgtttgat atggcgcacg ccaggaaaca
     2161 gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc cgtcagcagg
     2221 cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc aatcgcggtg
     2281 ggtagggctt tcggcgaacc cgccggggta aagccgctaa atgccaccat gtcgttatgc
     2341 tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc catttttact
     2401 ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg aggaaagcgt
     2461 tcattccttc ctggtaatct tcgctgtcat aaaccgcgcg tcgcatcccc tgaatacgtt
     2521 caaattcatc ggagttcatg gtgtgcgctt cgcccagtac acgcagctct tctttgataa
     2581 cagcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag gtgaaatctt
     2641 ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg cgctgggcgg
     2701 tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg tcgcgggtca
     2761 ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc attgagaagg
     2821 ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat gcgccacccc
     2881 aaacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc atgcgggtga
     2941 tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc agttcgtgaa
     3001 tatcgtgacc tgcggagaag actttggatc cactcggcgc gcgcaaaatg atacagcgaa
     3061 tttccggccg gttgagatcg cttaacgcct gcataagatc atcaataaag actttactta
     3121 aggcattaag ttttcggcca tagttaaact caatgaccgc cactttgttg atagtggcaa
     3181 cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt ctggataaat
     3241 tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac cgctaaaagc
     3301 gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag tacttcttct
     3361 tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg taaacgacca
     3421 ctggcagtta gcgcggtttt gaactcgatg atggcgtgcc agatctcatc gattccacgt
     3481 ttttccagtg cgctacaagt cagaacccgt ggctgccatt cgtcgtattt acgtcgcaga
     3541 atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg gttatcgcca
     3601 tcgtctttgt tgataacgat cagatcagcc acttccatca gccctttttt aatgccctgc
     3661 agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat gcgggcgact
     3721 tctgtttccg actgcccgac gccaaccgtt tcgacaatca ctacgtcata acccgccgct
     3781 tcgcataaca gcattaattc ccgcgctcgc tgactggcac cgcccagatg accggaggat
     3841 ggtaccgggc gaataaacgc cgcttcggca cgcgccaggt cattcatgcg gattttatcc
     3901 ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac cgcgaccttt
     3961 aatccctctc gaatcaacaa catgccaaag gcctcaagaa aggtactttt ccccgcgctg
     4021 ggggtgccgg taacgcccag tcgcagggtg ttaccgcagt acggcataat ggcatcaagc
     4081 agctgcgtac ttagtgcctg atgacgcggg tgacggcttt ccaccagcgt catggcctgg
     4141 gcgagtgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgccag cgtggcttca
     4201 ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga gcataggtgt
     4261 acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat cctgcggtgg
     4321 aatgacgcca ccggcgacta cgcagatatc ttcgcgtccc cattttttca gcgcctcgac
     4381 cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca ctacgtgaac
     4441 gtcattttct actgccaggc gggcgatctc ttcaggtgta gagaacatcg ggcttaaatc
     4501 tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgagccgc gatcgtgccc
     4561 atcctggccc attttggcga tcagaatgcg caggcgacga ccattgtcgg caaggaactg
     4621 ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagatt gatgatagct
     4681 ttgcgcaatc acgccggtaa cacactggct tggcaccaga taacggtcga aagcggcttc
     4741 cagcgcatcg gaaatttcac ccagggtggc gcgaacgcga gcggcattaa cagcggcagc
     4801 cagcaggttt tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg cggttacggc
     4861 ggcatcatca cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt tacgcaccat
     4921 cacgttgtcg atctcaagta catcggtttc gtcttcgtga tccagtttgt acttgttgac
     4981 accaacgatg acacgtttgc cctggtcgat aagcgactgt tcgcgcgctg aggcctattc
     5041 gatcattcgt tttggcagac ctgcttcgat cgctttcgcc ataccaccgg cttcgtcgat
     5101 ctgttggata atagctctgg cttgtttgac gatttgatcg gtcagcgact caatgtaata
     5161 ggatccggcc agtggatcga cggtgcggca gagttctgat tcttcctgga tgatgatctg
     5221 ggtgttgcgg gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt cgtcaaaggc
     5281 gttggtatgc agtgactgag taccgcccag cgtcgcagcc agtgcttcaa tggtggtgcg
     5341 gataacgttg ttatacggat cctgttcagt caggctccag cctgaggtct ggcagtgggt
     5401 acgcagcgcc agtgatttcg ggtcctgtgc gccaaatcca ctgaccgctt cgctccatca
     5461 ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga tccatgccga tgccgaagaa
     5521 gaacgacagg cgaggagcga agtcatcaat tttcagtccg gcagagattg ctgctttgat
     5581 gtactcaatc ccatcagcga gcgtaaatgc tacctgctgc acgcagttgg cacccgcttc
     5641 acccatgtgg taaccgctga tactgatggt attaaatcgc ggcatgttgc cggaacacca
     5701 ggcgatgatg tcggcgataa tgcgcattga cggttttggc gggtaaatat aggtgttgcg
     5761 gcagaggtac tctttgagga tatcgttctg aatggtgccg gtcagtttat caggtgtaac
     5821 accttgctct tccgcggcga cgatataaaa cgccagtact ggtagcactg cgccattcat
     5881 ggtcatcgaa accgacattt tatccagcgg gatctggtcg aacaggactt tcatatcttc
     5941 cacggtgtcg atagcgacgc ccgctttgcc gacgtcgccc gccacgcgcg ggttatcgga
     6001 gtcgtagcct cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt gcccggcggc
     6061 caggttacgg cgataaaaag cgttggactc ttttgctgtt gaaaaaccag catactgacg
     6121 gatggtccac ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt agggcggcaa
     6181 accaggaagg gtacctgtca cctccagatt atcgagatcg gcttcggtat acagcggctt
     6241 gatggtgatc ccttccgcgg tttgctgaac cagcgagtcg acagttttct cccgacggct
     6301 caattccttg ttggcaagct gttgccactc ctgcacgtta gacattattt gctccgtaaa
     6361 acgcaatcaa aatgagggta tttgcgccta cggtgagctt tttacgtggg tttgtcgcca
     6421 gcaaaaaaaa tgcattcaga cgtcgttttt tgccgcctga gatttgtcta ttttgtgatg
     6481 tgattcaagg aatacaaaaa gaagtgagga caacgcctga tatgtgctat caggcattat
     6541 ttgatggatt aatcctgacg aaggactttg tgaccataat cgagcaacgc atcagtgact
     6601 ttacgcatca tgcggctttc aggagcaaag cggtgccagt agagcatccg gcgttgaaat
     6661 agccctggcg ttaagtcaat cagttcaccg ctggctagtt ctttctcgat ttgcaggtgc
     6721 gggatcatac agcaggtggt gccctggcga gcaagttgca cgaacgcttc tgaagaatta
     6781 acgatatggc agggcacgct gcctggaggc agatcgaagt tttgctgcaa aaaggcctgg
     6841 tgcatatcgt caagatggtc aaaggcgacc acaggtgctt tcaataatgc cgaacgcgtt
     6901 acgccgttag ggaaatattt ttcggcaaac ggttttgagc tgacgaacag atagtcaagc
     6961 gcaccgagct tatcgacaag gcaactcggc agcgcctgat gttgaatact caccgcgccg
     7021 accacttcgc cgcggcgcag acgttcctga gtacgggttt catcttctac ctgcaagttg
     7081 aggcggatag gcgaatcagc caatacagga gccagtgccg gaagcaacca cgtcgccaga
     7141 ctgtcggcgt tgaccgccag tgaaagcagc agcggagtcg aaccggtttg ttcatcgccc
     7201 agccactctt cttccagcaa ctccacctgg cgcagcagtg ccagcagttt ttgcccttgt
     7261 tccgtcgggc gcggcggtac ggtacgcacc aacagcggct gcccgaacat attttccagt
     7321 tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc cgcgcgctca
     7381 aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc cgggcgtttc
     7441 attgctgttg tctgctcctg gtaaatgctt tttcctgcac tatgacacaa ttttgtgtca
     7501 ggttgcatat accgcagccg tcggcgggta acatttgtat caatggttaa acgcgcagct
     7561 taaaagatca gggatttgcg ctattgcatt tcgcttagcg acttcgcgcc ctggttatgt
     7621 tgaatggcgt ggcgttattg cctctgttcg caccgtggag cggcataccc agaccgttgt
     7681 atgctttatt tgcggggcga aaggggatgc ccgccattgc gcgatatttt atggatgagt
     7741 taaccacccg gcttgccaac ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa
     7801 tttcatacca caggcgaaac gatcatgacg caggatgaat tgaaaaaagc agtaggatgg
     7861 gcggcacttc agtatgttca gcccggcacc attgttggtg taggtacagg ttccaccgcc
     7921 gcacacttta ttgacgcgct cggcacgatg aaaggccaga ttgaaggggc cgtttccagt
     7981 tcagatgctt ccactgaaaa actgaaaagc ctcggcattc acgtttttga tctcaacgaa
     8041 gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa tcaacggcca catgcaaatg
     8101 atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt tgcggaaaaa
     8161 tttatctgta ttgccgacgc ctccaagcag gttgatattc tgggtaaatt cccgctgcca
     8221 gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa actgggcggt
     8281 cgtccggaat accgtcaggg cgtggtgacc gataatggca acgtgatcct cgacgtccac
     8341 ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg
     8401 gtgactgttg gcttgtttgc taaccgtggc gcggacgttg cgctgattgg cacacctgac
     8461 ggtgtcaaaa ccattgtgaa atgatctgac gggggaacct cccccgttaa aaaaattctc
     8521 ttcattaaat ttggtgacat gtgtcacgtt tttacctggc tattgtcgat tgctctaaat
     8581 aaatcctcta aaccagcata ttcaaccaag aaataccttt gcgtgatatt tcctcaacat
     8641 cgcgacgcaa acgttcatat tgccgcaata ttattttttg atatgttgaa aggcggatgc
     8701 aaatccgcac acaacatttc aaagacagga ttgggtaaat ggcaaaggta tcgctggaga
     8761 aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg gaaagccttc
     8821 gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat gaacaattaa
     8881 aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat ctgactgaag
     8941 acgtgatcaa cgccgcagaa aagctggtcg ctattggctg tttctgtata ggaacaaacc
     9001 aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca ccgttctcaa
     9061 atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg cgcggcgtgc
     9121 cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg ggttcttttg
     9181 aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg caattgggca
     9241 ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaat aaactgccgc
     9301 tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc gatgtggtga
     9361 gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa gaaatttcac
     9421 taatgaagcc cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg gatattccgg
     9481 cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac gtattcccga
     9541 cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc gacaacgtac
     9601 ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc ggcctggaag
     9661 ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg gtgaacttcc
     9721 cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac gaaaaccgtc
     9781 cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac atcgccgcgc
     9841 aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa gctgacgaag
     9901 acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt cgcgcccgtc
     9961 tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt gcccgttttt
    10021 ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc tgctccagaa
    10081 aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac gatggcggga
    10141 cagtatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg agaagggtta
    10201 tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga gcttattaac
    10261 agtcagtctc tggggaggag tcatcctccc ttacccttac tcactaaatt aggtcaaaga
    10321 atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac tttttgatag
    10381 ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa ttatctctgt
    10441 tttgcatggg gtttttcgaa cacgctcgaa gaaaagattt aaaggtagat caatgcgttg
    10501 tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg gtaaagccct
    10561 caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca tactgattgt
    10621 actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg agcaatcctc
    10681 ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag ggcgatgcag
    10741 gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata
    10801 tcccactctt caacggggag tttttccacc aactgacaat catgcgcata acccaccggt
    10861 tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc gcccattccc
    10921 aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc taatcgggaa
    10981 aggggtagca cgtcacgcac atccagtttt ggctcatgga ttttcaacct gttcatcacc
    11041 agttcgcttt gcggatggta attcaggaac agcaaattac cagcactaaa gggatgcaaa
    11101 actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg ctgggtgtcg
    11161 agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac cgggggataa
    11221 gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg cgttaacgca
    11281 cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagttct
    11341 ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc
    11401 tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg
    11461 acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc
    11521 tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac
    11581 cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt
    11641 gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg
    11701 ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata ttcaatgcgg
    11761 caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc
    11821 gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca
    11881 cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct
    11941 gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata
    12001 gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca
    12061 tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct
    12121 ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac ccctgccgag
    12181 atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc atggctgccg
    12241 ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcactg
    12301 cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt
    12361 tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt ggcaggttgg
    12421 gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggataa agtgaccggc
    12481 gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta cgacgaagac
    12541 gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt tcgtgttgcc
    12601 gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa
    12661 ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca
    12721 agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct
    12781 gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa
    12841 cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg tgctgattaa
    12901 aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc tgacggcgga
    12961 gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg
    13021 cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg gcctggatgt
    13081 ggtttaccat gcccagggcg aatatgcata tgctgatgaa atcatgaaca gtgcgctgga
    13141 aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga tcgactggcg
    13201 tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg tactccgccg
    13261 cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc gtccgggaat
    13321 gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccaca gtgcgcgcta
    13381 tccgtcctat aacaccattg tcggcagcgg tgaaaacggc tgcattctgc actacaccga
    13441 aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt gtgaatacaa
    13501 aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca cccaggccca
    13561 gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc tgtatcgtcc
    13621 gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta gcggcctggt
    13681 aaaactcggc attctgaaag gtgatgttga tgaactgatc gctcagaatg cccatcgtcc
    13741 tttctttatg catggcctta gccactggtt agggctagat gtccatgacg ttggcgttta
    13801 tggtcaggat cgctcgcgca ttctggaacc gggcatggta ctgaccgtag agccagggct
    13861 gtatattgcg ccggatgcag aagtgccaga acaatatcgc ggtatcggca ttcgtattga
    13921 agacgacatt gtgattaccg aaaccggtaa cgaaaacctc accgccagcg tggtgaaaaa
    13981 gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc
    14041 ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg
    14101 ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat
    14161 ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtctgg
    14221 caatctctgg cggattgcgc aactgccatc accaccgtgc atgtcagcga tcgtggtcac
    14281 gctggatttg tcaccctcgc cgcagaagat taccaactgg cggcgctggg acaggttgtc
    14341 gaattgcaca atgtcgggca acggctgttt gcattgctgc gtaaagcacc tggcgtaacg
    14401 ctgcattgcc ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt tgaagtgacg
    14461 ctggaaagtg gcgagacgct gacgggccgc gtgctggtag cagctgatgg cacccattca
    14521 gcgttagcca ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca actggccgtg
    14581 attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg cttcacgcaa
    14641 catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt ctggtgtcat
    14701 ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc
    14761 cagtcggcct ttggctggcg acttggaaaa attacccacg ccggtaaacg cagtgcttat
    14821 ccgctggcgt taacccgcgc cgccagagct attacccatc gtaccgtgct ggtgggcaat
    14881 gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat gcgagatgtc
    14941 atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat cggtgattac
    15001 ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac catcggcgtc
    15061 acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt cgggcgcaac
    15121 atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc gcagcgcacc
    15181 ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaat gtgttgatgt
    15241 agccattgtt ggtggcggca tggtggggct ggcggttgcc tgcggcttac aggggagcgg
    15301 cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcgg atgcaccacc
    15361 acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt
    15421 ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag tgtgggacaa
    15481 agacagtttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc atcttgggca
    15541 tatcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga
    15601 tatcactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa atgaaacttt
    15661 cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg cggacggtgc
    15721 taattcctgg ttgcgcaaca aagcagatat ttcgctgact ttctgggatt atcagcatca
    15781 cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc ggcaggtttt
    15841 ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcaattgt
    15901 ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag atgaatttaa
    15961 tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg agagcgcgcg
    16021 tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc gtctggtgct
    16081 agtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggagtaa atctcggctt
    16141 tatggatgct gcagagctga ttgctgaact gaaacggttg catcgtcagg ggaaagacat
    16201 cgggcagtac atttacctgc gccggtatga gcgtagccgc aagcacagtg cggcgctgat
    16261 gctggctggt atgcagggat tccgcgatct gttttccggt accaatccgg cgaaaaaact
    16321 gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaaac cacaacttat
    16381 ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcttctg
    16441 ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg agattttcct
    16501 catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat gtcattattt
    16561 tttgatttag tgttttttga cattttttta gctcttaata ttgccttatt caaattagct
    16621 ttctaatggc atcatcttta tatagaaact ggtgtgtttt ttggtttttt attctgtcgc
    16681 gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca
    16741 gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc
    16801 tccgatgctg ggtttcgtgg tgataatttc accatgaaaa gttgtcagcc ccgcttattc
    16861 aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct ttgcggcgct
    16921 cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca aatcgacgaa
    16981 catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac catcgtcgat
    17041 ctccgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga tgtggcgaag
    17101 ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg cggtgtgata
    17161 gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt taactccgcc
    17221 acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg catcgaaatt
    17281 accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca ggcaaaagct
    17341 gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc gttctttggc
    17401 gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc gggctatgaa
    17461 attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga agcgggtgtt
    17521 aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat gaatctttat
    17581 ggtcaggaga tggacgaaac tatttctcct ttagccgcca acatgggctg gactatcgcc
    17641 tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca gcgtgagcat
    17701 ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg taatgaactg
    17761 ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac cagcggtact
    17821 ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg tattggcgaa
    17881 acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa acctgttttt
    17941 gtgcgtaacg gcaaagccgt cgcgtgattt acttttttgg agattgattg atgagcaacg
    18001 taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa gccgacggca
    18061 cttacaccgt tggtattacc gaacatgctc aggagctgtt aggcgatatg gtgtttgttg
    18121 acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt gccgaatcag
    18181 taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg gtaaacgacg
    18241 cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggtggc tggatcttta
    18301 aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc gcatacgaag
    18361 cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat cagggtgagg
    18421 aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat tcactgcacg
    18481 tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag cggcgctttt
    18541 attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggc
    18601 gcacaatcgt taaacgcgct gaccggccag attgtaccga aagatattca gcttgcgacc
    18661 ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact caaggctatt
    18721 gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagcta
    18781 ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtacactccg
    18841 tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca gcaggtaacg
    18901 ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc caccgctgcc
    18961 gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc
    19021 gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt
    19081 ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga cgtcttcggc
    19141 gtgctgttac agcaggtagg cactaccggt gaaattcacg actacactgc gcttattagc
    19201 gaactgaaat cacgcaaaat tgtggtcagc gttgccgccg atattatggc gctggtgctg
    19261 ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgcttcggc
    19321 gtgccgatgg gctacggtgg cccacacgcg gcattctttg cggcgaaaga tgaatacaaa
    19381 cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg
    19441 cgcatggcga tgcagactcg cgagcaacat atccgccgtg agaaagcgaa ctccaacatt
    19501 tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg
    19561 gttggcctga aacgtatcgc taaccgcatt caccgtttga ccgatatcct ggcggcaggc
    19621 ctgcaacaaa aaggtctgaa gctgcgccat gcgcactatt tcgacactct gtgtgtggaa
    19681 gtggccgaca aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa cctgcgtagc
    19741 gatatcctga acgcggttgg gatcaccctt gatgaaacca ccacgcgtga aaacgtaatg
    19801 cagcttttca gcgtgctgct gggcgataac cacgggctgg agatcgacac gctggacaaa
    19861 gacgtggctc acgacagccg ctctattcag cctgcgatgc tgcgcgacga cgaaatcctc
    19921 actcatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg
    19981 ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg
    20041 aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc cgaactgcac
    20101 ccgttttgcc cgccggaaca ggctgaaggt tatcagcaga tgattgcgca gctggctgac
    20161 tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc cggcgcacag
    20221 ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa cgaagggcat
    20281 cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc tgcacatatg
    20341 gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga tctgactgat
    20401 ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt gacttatccc
    20461 tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc
    20521 ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg
    20581 ggctttattg gcgcggacgt ttcgcacctc aacctgcata aaactttctg cattccgcac
    20641 ggcggtggtg gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc accgtttgta
    20701 ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc agtttctgcg
    20761 gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc
    20821 gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc
    20881 cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa
    20941 tgtattctcg atattcgccc gctgaaagaa gaaaccagca tcagcgagct ggatattgcc
    21001 aagcgcctga tcgactacgg tttccacgcg ccgacgatgt cgttcccggt ggcgggtacg
    21061 ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg
    21121 atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc gctggaagat
    21181 aacccgctgg tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg ggcgcatccg
    21241 tacagccatg aagttgcggt attcccggca ggcgtggcag acaaatactg gccgacggtg
    21301 aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt accgattagc
    21361 gaataccagt aattcactga ttcgactatt ttctaaaggc gcttcggcgc ctttttagtc
    21421 agatgacaaa gtacaaaagt gctcagacag tcccctcgcc cctttgggga gagggttagg
    21481 gtgaggggaa caggccggca ctggcgcgaa tatttaccct caccccggcc ctctccctga
    21541 aagggcgagg ggaaaagcgt gccaacattg aagattgagc cagtttgtta gcaatctcaa
    21601 agatacgtca acgaattaat ttttctcgga aaaacaaatg gctatagcac ttgtgaccgg
    21661 tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgaaagaag agtatacggt
    21721 ggcggttaat tatcaacaaa acctccatgc ggcgcaggaa gtggtgaact taatcacgca
    21781 agccggtggc aaggcattcg tgctccaggc ggatatcagc gacgaaaatc aggtcatcgc
    21841 aatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca ataacgccgg
    21901 gatcttgttt acccagtgca ccgttgaaaa ccttaccgca gagcgaatca accgagtact
    21961 ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac gcatggcgct
    22021 taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct cacggttggg
    22081 ttcgccaggg gaataggttg attacgcggc atcgaaaggg gcgattgata cgttaaccac
    22141 cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc ggccagggtt
    22201 tatttatacc gaaatgcacg ccagcggcgg cgagcttgga cgcgtcgatc gcgttaagtc
    22261 gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct
    22321 actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata
    22381 aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta
    22441 aagcttctcg ccgttgctgg caatcacctc tttgtaccag ttaaagctct tcttacgtga
    22501 acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt
    22561 gctgtactgc ccggtagtga atgacacgca gtcgatgcag ccccacggtg tgtagcccat
    22621 cagatccaca ccatcgtaag tcactgcttt tttcatctct tcaatatggg cgcgcaggta
    22681 gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa
    22741 accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata
    22801 gcgcagacct actggatcaa tctgccagca ccagtcagac gctttaacat acgggttcgg
    22861 tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt
    22921 gatcatgtaa tagctgaaac caagataatc gcaggtgcct tcacgcagta catccagatc
    22981 gccatcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca cataggacgg
    23041 gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc
    23101 gaacatcaca tcgtccgggt tacaggagta aggatagagc ggcaccatcg ccagcataca
    23161 gccgactttc atctccgggt taatgcgacg cgcggctttc actgctaggg cgctggcgac
    23221 aaactggtga tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct cggtatacac
    23281 cacgccggag cagcagtaac caaacagcgg cgcacgccag ttacgctggt tgtttatttc
    23341 gttgaaggtc atccagtatt tgactttatg cttgtagcgt tcgaatacca cttccgcgaa
    23401 acggacaaag aaatcaacga ctttacggtt ggtccagcta ccgtattgct gcaccagatg
    23461 cagcggtatt tcaaagtggg agagggttat caccggttcg atgttgtatt tcagcagttc
    23521 atcgaacaga gagtcgtaaa acttcagccc ttcttcgttt ggctgagctt catcaccttt
    23581 tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa
    23641 tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta
    23701 ttttcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag
    23761 aacgtcacaa atgctcggtc ctttgccgcc tttgttccag ccgccttcga cctgatgagc
    23821 ggcaacggcg ccaccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg
    23881 ctccagatga gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt
    23941 ctcaacgttt ccaggatgac attctggctg ggcgtaaaac catcaccatc cgcgacgagt
    24001 ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg acgttttgaa gatgacggtt
    24061 atttttgcac gattgaagtc accgcaacct caaccgtaac gctggatacg ctgacggaaa
    24121 aacatgcaga gcaggaaaat atgaccctga ctgaactgat aaaggtcatt gccgacatct
    24181 atcccggtca gacacagttt tatgtgattg aatttaaatg tctttaaatt gaactcacgc
    24241 ctgttagttg aaatttctca tttaatttca tgatttttag gagattagtc tcaaatcaga
    24301 atttatttta gctaacaggt attcactgga actattctca gttacgctgg agaggtatac
    24361 atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat taagcaggga
    24421 tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt
    24481 atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag cgccacagcg
    24541 ataaccagct acagcctcta tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc
    24601 tatcacgcca ttcctcatca acgggcaaaa atgtggctga agaaatttga ccattgcgct
    24661 atttacctgt tgattgccgg aacctacacg ccgtttttgc tggtggggct ggattctccg
    24721 ttagcgcgcg ggttgatgat tgttatctgg agcctggcat tgctggggat tctgtttaaa
    24781 ctgaccatcg cgcaccgatt caaaatttta tctctggtga cctatctggc gatgggctgg
    24841 ctgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcgggcag cgttacctta
    24901 ctggcggtag gcggcgtggt ttattcgctc ggagtgattt tctatgtctg caaacgcatt
    24961 ccctacaatc atgccatctg gcacggcttc gtgctcggcg gcagtatgtg ccactttctg
    25021 gcgatctatt tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc tgataagacg
    25081 cgccagcgtc gcatcaggcg ataaatgcct gatgcgatac agagatttac tcttcgagtg
    25141 aatacggcag cggctcgata tgcagtgtat tcgcatcgtc gcgtacgcgg aagatgctgt
    25201 ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct tccagtttta
    25261 ccgcagccag caccgtaccg gtacgacgcc agttctcgcc cattttcagc tctaagtctt
    25321 caccagcttc cggcagtcgg ctggcgctac ctgccagcaa ccagagcgca cgtttattgg
    25381 caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct ttcttaaaac
    25441 tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg ctgttcgcgg
    25501 catcaatcac cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg ctattgttca
    25561 gttccgcttc accgcgcagt ttatcggtca gcatattggc agtagcttca tcggttacga
    25621 tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct tctttaacta
    25681 cctgtttttc tctcgaaggt agttcactaa agagattggc gagcgcggcg cgcgcctgaa
    25741 aaccggcaac accaagcagc acacgctcgt cgtctgccgc gatggtcact ttagagaaca
    25801 ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt tcaatccacg
    25861 caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct ttggcgtcgc
    25921 aatgggcggc gagcaggtgc tgattttctg tcatctggct gacatctgct gtcacctgac
    25981 cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag tcatcaagcg
    26041 tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acggggagga aaaggtgtaa
    26101 aagccataat agagtcctga gttgcttaac ggcgtattaa taactaatgg taaaagagct
    26161 atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc gacttccaaa
    26221 aaaatgaagc tcttgaattg ttgtgaatta atttcgcgat cgcgatggtg tttactgata
    26281 ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg gtactggatt ttcgtgagac
    26341 aggaagaaga tggacattaa caacaaagcc cgcattcatt gggcatgccg ccgtggtatg
    26401 cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag cttaagcgat
    26461 gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct gtttaactgg
    26521 ctgatgaatc acggtaaacc ggccgatgca gaactggaaa tgatggtccg actcatccag
    26581 acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca
    26641 gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca tgccctggcc
    26701 gctcagttac accccgttat ggatggtgtt actttcgctg gtggtatttg attgcgttcg
    26761 cagccagcgg cgtattaatg ctcgccaggg ggaaattcga ttgttgatgg acgggcgttt
    26821 gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta agagcggcat
    26881 gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg cagccgacag
    26941 catggacgaa gctgaatggc gggatttacg gcggattttg ttgcaacaag agacgcaaag
    27001 ataaatatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg taatgctctg
    27061 ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact
    27121 ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc accggtttcg
    27181 ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg cctttggtcg
    27241 agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat cccagttgat
    27301 cgccaagccc atacagcgca acaattttac cttcaaggtt caggtcgtcg agctgatccc
    27361 agacggcttc ccagtcttcc tggatttcgc caaaatccca ggtcgggata cccagaatga
    27421 gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt aaggtcacca
    27481 gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa caggtgctgg
    27541 aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt ggcttttgcg
    27601 gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat caaatgagtg
    27661 accataaggg gcgcaagtga aacaggatct ggcacgcatc gagcagtttc ttgatgctct
    27721 gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat
    27781 gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa gtgacgattt
    27841 gcaggcatta ctggcagaac ggctagaggg cgggtataaa gccaccagct cagcgcgttt
    27901 gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc gtgaagacga
    27961 tcccagtgcg catctcgctt caccgaaatt gccacagcgt ttgccaaaag atttaagcga
    28021 agcgcaggtc gaacgtttat tacaggcacc attaattgat cagccactgg agctacgcga
    28081 taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac tggtcgggct
    28141 gacaatgagt gatatcagcc tacgtcaggg cgtagtgcgg gtcattggta aaggcaacaa
    28201 agagcgcctg gtgccgttag gtgaagaggc cgtttactgg ctggaaacct atctggaaca
    28261 tgggcgtccg tggctgttga atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc
    28321 gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgccg tgctggcggg
    28381 tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca ctcatttatt
    28441 aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg atctctccac
    28501 cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc aacagcatca
    28561 cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt
    28621 tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa ttcaacaaac
    28681 gttagccaaa atgggcatta aaagcagcga tattcagcca gcacccgtgg ctggcatgaa
    28741 gactgtgctg actaacagcg gtgtgttgta catcaccgat gacggtaaac atatcattca
    28801 ggggccaatg tatgacgtta gtggcgcggc tccggtcaat gtcaccaata aaatgctgct
    28861 aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca
    28921 cgtcatcacc gtgtttactg atattacctg tgggtactgc cacaaactgc atgagcaaat
    28981 ggcagactac aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc gccaggggct
    29041 ggagagccag gctgagcaac aaatgaaagc catctggtgt gcgaaagata aaaacaaagc
    29101 gtttgatgat gtgatggctg gtaaagctgc cactccggca agttgcgata tcgatattgc
    29161 cgatcattac gcacttggcg tccagcttgg cgttagcggc actccggcaa ttgtgctgag
    29221 caatggcaca cttgttccgg gttaccagcc gccgaaagag atgaaagaat tcctcgacga
    29281 acaccaaaaa atgaccagcg gtaattaatt cgcgtgaaac aacagataca acttcgtcgc
    29341 cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct gcgccgttta
    29401 tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa aggtatgttg
    29461 ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc
    29521 gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac cagcacggct
    29581 ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct ggtaccaaac
    29641 cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca tgcccgtggc
    29701 gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggggt tgaacacgct
    29761 cgctcgttgg gcattccggt tattgttacc gatcaccatt tgccaggcga aacattaccc
    29821 gcggcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc gaaatcactg
    29881 gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag
    29941 ggctggtttg atgagcgcgg catcgcaatt cctaacctgg cagaactgct ggatctggtc
    30001 gcgctgggga cagtggcgga cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg
    30061 caggggatga gtcgcatccg tgccggaaag tgccgtccag ggattaaagc gctgctggaa
    30121 gtggcaaacc gtgatgcaca aaaactcgcc gccagcgatt taggttttgc gctggggcca
    30181 cgtctcaatg ctgccgggcg actggacgat atgtccgtcg gtgtggcgct gttgctgtgc
    30241 gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa tcagacgcga
    30301 aaagagatcg aacaaggaat gcagattgaa gccttgaccc tgtgcgagaa actggagcga
    30361 agtcgcgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca tcagggcgtt
    30421 gtcggtattc tggcttcgcg catcaaagag cgttttcacc gtccggttat cgcctttgcg
    30481 ccagcaggtg atggtacgct gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt
    30541 gatgcgctgg agcgattaga cacactctac cccggtatga tgctgaagtt tggcggtcat
    30601 gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca acaacggttt
    30661 ggcgagctgg ttaccgagtg gctggaccct tcgctattgc aaggcgaagt ggtgtcagac
    30721 ggcccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg cgatgctggc
    30781 ccgtgggggc agatgttccc ggagccgctg tttgatggtc atttccgtct gctgcaacag
    30841 cggctggtgg gcgaacgtca tttgaaagtc atggtcgaac cggtcggcgg cggtccgctg
    30901 ctggatggta ttgcttttaa tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa
    30961 gtgcaactgg cttacaagct cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt
    31021 atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga gcgtggattg
    31081 ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa tcagaccatg
    31141 tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc cgacgttctt
    31201 agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa acgccgagct
    31261 ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta aagagcgttc
    31321 ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg aagatgtttc
    31381 tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg aagccgttgc
    31441 tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta tgttctctgg
    31501 cgaatatgac agcgccgact gctacctcga tattcaggcg gggtctggcg gtacggaagc
    31561 acaggactgg gcgagcatgc ttgagcgtat gtatttgcgc tgggcagaat cgcgtggttt
    31621 caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat
    31681 caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg ttcaccgcct
    31741 ggtgcgtaag agcccgtttg actccggcgg tcgtcgccac acgtcgttca gctccgcgtt
    31801 tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg atctgcgcat
    31861 tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt
    31921 gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc gttcccagca
    31981 caagaataaa gaccaggcca tgaagcagat gaaagcgaag ctttatgaac tggagatgca
    32041 gaagaaaaat gctgagaaac aggcgatgga agataacaaa tccgacatcg gctggggcag
    32101 ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca ccggggttga
    32161 aacccgcaat acgcaggccg tgctggacgg cagcctggat caatttatcg aagcaagttt
    32221 gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt
    32281 agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc tgcgtgagca
    32341 gggaattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat tgcacgcaga
    32401 attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg ccgttgctgg
    32461 ccgcatgatg acccgccgta ttatgggtaa agcctctttt gttaccctgc aggacgttgg
    32521 cggtcgtatt cagctgtacg ttgcccgtga cgatcttccg gaaggcgttt acaacgagca
    32581 gttcaaaaaa tgggatctcg gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa
    32641 aaccggcgaa ctgtctatcc actgcaccga gctgcgtctg ctgaccaaag cactgcgtcc
    32701 gctgccggac aaattccacg gcttgcagga tcaggaagcg cgctatcgtc agcgttatct
    32761 ggatctcatc tctaacgatg aatcccgcaa cacctttaaa gtgcgttcgc agatcctctc
    32821 tggcattcgc cagttcatgg taaatcgcgg ctttatggaa gttgaaacgc cgatgatgca
    32881 ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc catcataacg cgctggatct
    32941 tgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg tcggtggctt
    33001 cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg tacgtcataa
    33061 cccagagttc accatgatgg aactctatat ggcttacgca gattacaaag atctgatcga
    33121 gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga cggaagtcac
    33181 ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca tgcgtgaagc
    33241 gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gacaacttcg actctgcaaa
    33301 agcgattgct gaatctatcg gcatccacgt tgagaagagc tggggtctgg gccgtatcgt
    33361 taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct tcattactaa
    33421 atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg aaatcacaga
    33481 ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg agctgaatga
    33541 cgcggaagat caggcgcagc gcttcctgga tcaggttgcc gcgaaagacg caggtgacga
    33601 cgaagcgatg ttctacgacg aagattacgt caccgcactg gaacacggct taccgccgac
    33661 agcaggtctg ggaattggta tcgaccgtat ggtaatgctg ttcaccaaca gccataccat
    33721 ccgcgacgtt attctgttcc cggcgatgcg tccagtaaaa taagcattac gttatgctaa
    33781 taaccccggc aaatgtcggg gttttttaat tgtgctgcgc gaaagcagac aacaattttc
    33841 ttgcttcact attggctgcc tgcattacca tccacggact gaacgcccac ggcgtggcat
    33901 caataccgtg taatacatct gctaaatcac accattgata atccatcact tcatcatcgt
    33961 tgatctgtaa cgcactgttg gtgcgtgcgg caaataccgg acacacttca ttttccacaa
    34021 tgccattcgg atcggtggcg cgatagcgaa agtcaggata gatagattca ggaggcgtaa
    34081 tttccacgcc aagctcataa cggcaacggc ggatcactgc gtcttcgttg ctttctccca
    34141 gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccatgctttt ttgctcagtg
    34201 cgcggcgggt aactaataat tgccccttgg cattaaacag ccaactggag aacgcgagat
    34261 gtaagagggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg ggaactccct
    34321 gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa ttctgatcac
    34381 tcatcattat agataattga agagtgaaat gattaaccaa gtgagcgttt atcgacaacc
    34441 gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca tcatatattt
    34501 gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag agagagtaaa
    34561 aagcaacagc agactgaata tagcgcgcat taaaagattt atattattct ccatgctcat
    34621 ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgactttt aaatctttat
    34681 cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag aagacgttca
    34741 gcacaacggc gctaagggtt gcgagcataa tgccgctatg tagcagcggt tgcagtacgg
    34801 ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg ccgagactga
    34861 tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac agaattcgaa
    34921 tccctgtcgc cagtaccatg ccgaacatca ctagaccagc gccgcccagc acaaattgcg
    34981 gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt aatataattc
    35041 ccgaagaaat acacacccag cggctatgaa cgcgcgtcac gctaaccagg ccaacgtttt
    35101 gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc tatcattgtc cctacgccat
    35161 cgacacgcag cccgcgaata atatcgtgtg aagagagttt acgaccgact atttcaccca
    35221 gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca gtcatggtca
    35281 gaatggaaac gggatcgaaa atcggcatac caaacgacat cggggtgaca atcgcaaacc
    35341 acaaggcatc atgtagcccg gataaattga cttcattcat catccacgaa agtaaaaagc
    35401 caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca tagcgagtaa
    35461 tgagcaagat aaaaattaag acggcaaagg agatacctga ataaacggga ttaccatatt
    35521 gcggattccc tttacctccg gcggcccagt caatacccac ctgaatgatg ctaagtccga
    35581 cagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag cgaccgataa
    35641 gtggcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat atccccagca
    35701 ggccaatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca aaggtcaccg
    35761 acataatcac cggcaggcgg atccccataa agcggccgat accgatacat tgcaataatg
    35821 tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagct tcttttgaga
    35881 ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac atgacaagga
    35941 cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca acctcatcag
    36001 ttgggcggtc ttgcccagaa gatgagagaa gttgggaatc tatggcgctc atcagtgttt
    36061 ctcctttcgc ccgccagagt aaaaagcaac cgtttacgtt aatgaaactg tctaaacaca
    36121 tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat tgcggaggca
    36181 atcacataat cgtggttaaa ccacgaaaat gagaagagaa cctgacaatg gaattatctg
    36241 taattttctc atattgatat ttcattaatc attctggcat gtcgtactct cgcatttaat
    36301 cgtttttatc tggatagcac tcttttgatc ggcaatgttg aaataatgaa agcggcatag
    36361 attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt catgctacct
    36421 aatatatgcc cgtt
//
LOCUS       DAJVTY010000020        36083 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVTY010000020 DAJVTY010000000
VERSION     DAJVTY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36083
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.020"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(51..1700)
                     /gene="actP"
                     /locus_tag="OQQ66_000922"
     CDS             complement(51..1700)
                     /gene="actP"
                     /locus_tag="OQQ66_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1903935.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1697..2011)
                     /locus_tag="OQQ66_000923"
     CDS             complement(1697..2011)
                     /locus_tag="OQQ66_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1903936.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2143..4102)
                     /gene="acs"
                     /locus_tag="OQQ66_000924"
                     /pseudo
     CDS             complement(2143..4102)
                     /gene="acs"
                     /locus_tag="OQQ66_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4495..5931
                     /gene="nrfA"
                     /locus_tag="OQQ66_000925"
     CDS             4495..5931
                     /gene="nrfA"
                     /locus_tag="OQQ66_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1903937.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5976..6542
                     /gene="nrfB"
                     /locus_tag="OQQ66_000926"
     CDS             5976..6542
                     /gene="nrfB"
                     /locus_tag="OQQ66_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1903938.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6539..7210
                     /gene="nrfC"
                     /locus_tag="OQQ66_000927"
     CDS             6539..7210
                     /gene="nrfC"
                     /locus_tag="OQQ66_000927"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1903939.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7207..8163
                     /gene="nrfD"
                     /locus_tag="OQQ66_000928"
     CDS             7207..8163
                     /gene="nrfD"
                     /locus_tag="OQQ66_000928"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1903940.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8183..9901
                     /locus_tag="OQQ66_000929"
     CDS             8183..9901
                     /locus_tag="OQQ66_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1903941.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9894..10277
                     /gene="nrfF"
                     /locus_tag="OQQ66_000930"
     CDS             9894..10277
                     /gene="nrfF"
                     /locus_tag="OQQ66_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1903942.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10274..10870
                     /gene="nrfG"
                     /locus_tag="OQQ66_000931"
     CDS             10274..10870
                     /gene="nrfG"
                     /locus_tag="OQQ66_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1903943.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11211..12524
                     /gene="gltP"
                     /locus_tag="OQQ66_000932"
     CDS             11211..12524
                     /gene="gltP"
                     /locus_tag="OQQ66_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1903944.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12715..13346)
                     /locus_tag="OQQ66_000933"
                     /pseudo
     CDS             complement(12715..13346)
                     /locus_tag="OQQ66_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13440..15587)
                     /gene="fdhF"
                     /locus_tag="OQQ66_000934"
     CDS             complement(13440..15587)
                     /gene="fdhF"
                     /locus_tag="OQQ66_000934"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15168..15170),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1903945.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGSCNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15785..17251)
                     /gene="mdtP"
                     /locus_tag="OQQ66_000935"
     CDS             complement(15785..17251)
                     /gene="mdtP"
                     /locus_tag="OQQ66_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1903946.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17248..19299)
                     /gene="mdtO"
                     /locus_tag="OQQ66_000936"
     CDS             complement(17248..19299)
                     /gene="mdtO"
                     /locus_tag="OQQ66_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1903947.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19299..20330)
                     /gene="mdtN"
                     /locus_tag="OQQ66_000937"
     CDS             complement(19299..20330)
                     /gene="mdtN"
                     /locus_tag="OQQ66_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1903948.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20349..20624)
                     /locus_tag="OQQ66_000938"
     CDS             complement(20349..20624)
                     /locus_tag="OQQ66_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903949.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20833..22818)
                     /gene="yjcS"
                     /locus_tag="OQQ66_000939"
     CDS             complement(20833..22818)
                     /gene="yjcS"
                     /locus_tag="OQQ66_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS1903950.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSHRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23071..23388
                     /locus_tag="OQQ66_000940"
     CDS             23071..23388
                     /locus_tag="OQQ66_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS1903951.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23457..23786
                     /gene="yjdP"
                     /locus_tag="OQQ66_000941"
     CDS             23457..23786
                     /gene="yjdP"
                     /locus_tag="OQQ66_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS1903952.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23933..24691)
                     /gene="phnP"
                     /locus_tag="OQQ66_000942"
     CDS             complement(23933..24691)
                     /gene="phnP"
                     /locus_tag="OQQ66_000942"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS1903953.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(24693..25157)
                     /gene="phnO"
                     /locus_tag="OQQ66_000943"
     CDS             complement(24693..25157)
                     /gene="phnO"
                     /locus_tag="OQQ66_000943"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS1903954.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLN
                     VGSKLLAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25144..25701)
                     /gene="phnN"
                     /locus_tag="OQQ66_000944"
     CDS             complement(25144..25701)
                     /gene="phnN"
                     /locus_tag="OQQ66_000944"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS1903955.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25701..26837)
                     /gene="phnM"
                     /locus_tag="OQQ66_000945"
     CDS             complement(25701..26837)
                     /gene="phnM"
                     /locus_tag="OQQ66_000945"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS1903956.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(26834..27514)
                     /gene="phnL"
                     /locus_tag="OQQ66_000946"
     CDS             complement(26834..27514)
                     /gene="phnL"
                     /locus_tag="OQQ66_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS1903957.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(27625..28383)
                     /gene="phnK"
                     /locus_tag="OQQ66_000947"
     CDS             complement(27625..28383)
                     /gene="phnK"
                     /locus_tag="OQQ66_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS1903958.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28380..29225)
                     /gene="phnJ"
                     /locus_tag="OQQ66_000948"
     CDS             complement(28380..29225)
                     /gene="phnJ"
                     /locus_tag="OQQ66_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS1903959.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(29218..30276)
                     /gene="phnI"
                     /locus_tag="OQQ66_000949"
     CDS             complement(29218..30276)
                     /gene="phnI"
                     /locus_tag="OQQ66_000949"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS1903960.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            complement(30276..30860)
                     /gene="phnH"
                     /locus_tag="OQQ66_000950"
     CDS             complement(30276..30860)
                     /gene="phnH"
                     /locus_tag="OQQ66_000950"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS1903961.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30857..31309)
                     /gene="phnG"
                     /locus_tag="OQQ66_000951"
     CDS             complement(30857..31309)
                     /gene="phnG"
                     /locus_tag="OQQ66_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS1903962.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(31310..32035)
                     /gene="phnF"
                     /locus_tag="OQQ66_000952"
     CDS             complement(31310..32035)
                     /gene="phnF"
                     /locus_tag="OQQ66_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS1903963.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32056..32835)
                     /gene="phnE"
                     /locus_tag="OQQ66_000953"
     CDS             complement(32056..32835)
                     /gene="phnE"
                     /locus_tag="OQQ66_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS1903964.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(32961..33977)
                     /gene="phnD"
                     /locus_tag="OQQ66_000954"
     CDS             complement(32961..33977)
                     /gene="phnD"
                     /locus_tag="OQQ66_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1903965.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNDDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(34002..34790)
                     /gene="phnC"
                     /locus_tag="OQQ66_000955"
     CDS             complement(34002..34790)
                     /gene="phnC"
                     /locus_tag="OQQ66_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1903966.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34923..35366)
                     /gene="yjdN"
                     /locus_tag="OQQ66_000956"
     CDS             complement(34923..35366)
                     /gene="yjdN"
                     /locus_tag="OQQ66_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1903967.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(35526..35861)
                     /locus_tag="OQQ66_000957"
     CDS             complement(35526..35861)
                     /locus_tag="OQQ66_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1903968.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7785 a         9685 c        10209 g         8404 t
ORIGIN      
        1 gctggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
       61 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcacg gaacagttca
      121 cgctcgcgcg caccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
      181 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
      241 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcaacccc
      301 agccagccgc ccagcatcgt gccacgggta gtcagtttcg accagtacat ggaaagcaga
      361 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
      421 ttctgattct caaacagcat gccgaggata atcgcaatca cacccaaaat caggacggtg
      481 attttcgata cccgcagttc ttcacgttcg gtcgcacctt ttttgaagac gttagcgtac
      541 aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
      601 gtggcgaaag caaccgctga aataaaaccg aggaacaggt tgccgcccac tgcattcgcc
      661 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
      721 ggattcgcac caaccagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
      781 tagcccataa acccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
      841 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagaccc
      901 agagagagcg cggagatcgg atctttcacc agcccgcccg gcttcatgat gtcgacacct
      961 ttcgggtgta ccgccatcgc ttcgctgaac agattattga agctaaagcc gacgtgtttc
     1021 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacggcttt aataatttgc
     1081 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     1141 agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     1201 accatctggg cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     1261 cgaatcggcc cttgtttcag gcggtaagag gctacatcgg caaaggtgta gcgtcccagg
     1321 ttacgcagac gttcggcaat caggaacaaa atgatcggcc agcccaccag gaagcccagc
     1381 gagtaaatta agccgtcata gccggaggta aacaccagcg cggagatccc caaaaatgag
     1441 gcggcggaca tatagtcacc ggcaatcgcc agcccgttct ggaagcctgt gatattgccg
     1501 cctgccgtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     1561 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     1621 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     1681 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     1741 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     1801 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     1861 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     1921 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     1981 cttctatccg ctgataaata gtgccattca tcacagattc tccagaggta atgtagggat
     2041 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     2101 taggccggat aaggcgttta cgccgcatcc ggcaattgtg ggttacgatg gcatcgcgat
     2161 agcctgcttc tcttcaagca gcttctcgac tacgccagga tcggcaagcg tcgaggtatc
     2221 gcccaggttg ctggtatcgc ccgccgcaat tttgcgcaga atacggcgca taattttgcc
     2281 ggagcgggtt ttaggcaggg agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc
     2341 aatctcttta cgcacccagt tgcggacttc tgcgtacagt tctggtgacg gttcctcccc
     2401 gtgattaagc gtgacgtagg cgtagatcgc ctgaccttta atattgtgcg gaatacctac
     2461 tacggcggct tcggcaatct tcggatgcgc caccagcgcc gactcaatct ctgccgtccc
     2521 cagacggtga ccggagacgt tcagcacgtc gtccacacgc ccggtgatcc agtaatagcc
     2581 atcttcatcg cgacgcgcgc cgtcgccgct gaaatacata tttttgaagg tggagaagta
     2641 ggtctgttca aaacgttcgt gatcgccaaa cagcgtacgc gcctgacccg gccaggagtc
     2701 ggtgatcacc aggctgcctt cggtagcgcc ttccagcggg ttaccttcgt tatcgaccag
     2761 cgccggttgc acgccgaaga acggacgtgt tgccgaaccg gctttcagcc cggtagcgcc
     2821 aggcagtggc gtgatcatga aaccgccagt ttcggtctgc caccaggtat cgaccaccgg
     2881 acatttctcg ttgccgattt ttttccagta ccactcccac gcttccgggt taattggctc
     2941 gcccacggaa ccgagaatgc gcagcgacga acggtcggta ccctcgatcg ctttatcgcc
     3001 ttccgccatc agcgcgcgga tcgccgtggg tgcggtatag agaatattga cctgatgctt
     3061 gtccaccacc tgcgccatac gggcaggcgt cggccagttg ggtacgcctt caaacatcag
     3121 cgtggtcgca ccgcaggcca gcgggccgta cagcaagtaa ctgtgtccgg tcacccagcc
     3181 cacatcggcg gtgcaccagt agatatcacc cggatgataa tcaaagacat atttaaaggt
     3241 cagcgccgcg tacaccagat aaccgccggt agtatgcagc acaccttttg gcttaccggt
     3301 agaaccggag gtgtagagaa taaacagcgg atcttcggcg ttcatctctt ccgcttggtg
     3361 ctgatcgctc gcttgctcaa ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc
     3421 aattttcccg ccagtacgct tcagtaccac cacatgctct acgctggtga cgttcgggtt
     3481 tttcagcgcg tcatcaacgt ttttcttcag cggaatactg ccgcccggca cgcacacctt
     3541 cgtcggaagt gatcaccagt cgtgagttgg aatcaataat gcgcccggca acggcttccg
     3601 gcgagaagcc gccgaaaatc accgaatgca ccgcgccaat gcgggcgcag gccagcatcg
     3661 caaccgcggc ttccggcacc atcggcatat aaatcgccac cacatcacct tttttaatgc
     3721 ccagctcgag cagggtattg gcgaagcggc agacgtcgcg gtgcagctct ttatagctga
     3781 tatgtttgct ctggctgacg tcgtcgcctt cccagatgat ggcggtacga tcgccgtttt
     3841 cttgcagatg gcggtcaagg cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt
     3901 taatggacac attaccgggg gcaaaggagg tgtttttcac cttctggtaa ggtttgatcc
     3961 agtcaagaat ttttccctgt tcgccccaga aggtatcagg tacgttaata gattgttgat
     4021 acattgcctc gtactgctga gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg
     4081 tgtgtttgtg aatttggctc atgcttttgt tctccttgta gaatgttaat aatatgtgac
     4141 ataagcgtta aatgtagggg tattggcagt tttgtttagt atttgggcga cagatcacgc
     4201 aaaagtagaa ttgtgcaaat aaacggcagg gtaatttttg aaggtcagca acaaaagttg
     4261 attaattctt tgaggaacat gcagttatgc atgctgttga aaaagaggaa gatactgact
     4321 aactctaaag tggtatttta catgcactta caattgatta aagacaacat tcacagtgtg
     4381 gttatttgtt acacataggg gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt
     4441 gaaaaacaat aaaaaccgcc attgcaacaa tggcgcaatt cggatgaagc ccctatgaca
     4501 aggataaaaa taaacgcacg ccgtatcttc agcttattga ttcctttttt ctttttcact
     4561 tctgttcacg ctgaacaaac ggcagctccc gcaaaacctg taactgtgga agcgaagaat
     4621 gaaacctttg ccccgcagca tcccgatcaa tatctctcct ggaaagccac ctcggaacag
     4681 tcagagcgtg ttgacgccct ggcggaagat ccacggctgg tgatcctgtg ggcggggtat
     4741 cccttctcgc gcgattacaa caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt
     4801 gaaaccctgc gtaccggtgc gccgaaaaat gctgaagatg gtccgctacc gatggcgtgc
     4861 tggagttgta aaagcccgga tgtggcgcgt ctgatccaga aagacggcga agatggctac
     4921 ttccacggta agtgggcgcg cggcggcccg gaaatcgtca acaacttagg ttgtgccgac
     4981 tgccataaca ccgcctcacc agagttcgcc aaaggcaaac cggagttaac cctttcccgt
     5041 ccgtatgcgg ctcgcgcgat ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc
     5101 gaccagcaat cgatggtttg cggtcagtgc catgtggagt attacttcga cggcaaaaac
     5161 aaagcggtta aattcccgtg ggatgacggc atgaaagtcg aaaatatgga gcagtattac
     5221 gacaaaattg ccttctctga ctggactaac tccctgtcga aaacgccaat gctgaaagcg
     5281 cagcacccgg aatatgaaac ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt
     5341 atcgactgcc atatgccaaa agtgcagaac gccgaaggca aactctacac cgaccataaa
     5401 attggtaatc cgtttgataa cttcgcccag acttgtgcga actgccatac ccaggacaaa
     5461 gctgccttgc aaaaagtggt cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag
     5521 gttgaagatc aactggttca cgctcacttc gaagcgaaag cagcgctgga tgcaggcgcg
     5581 acggaagctg aaatgaagcc aattcaggac gatatccgtc atgcccagtg gcgctgggat
     5641 ctggcgatcg cttcccacgg cattcatatg cacgcaccgg aagaaggttt acggatgctc
     5701 ggtacggcga tggataaagc ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc
     5761 aaaggcatca cccatgaaat ccagatcccg gatatctcaa ccaaagagaa agcccagcag
     5821 gccattggcc tgaacatgga acaaatcaag gccgagaagc aggacttcat caaaacggtg
     5881 atcccgcagt gggaagaaca ggcacgtaaa aacggtctgt taagccaata accccgttcc
     5941 gcctcgcaag gggcggaaaa cacaatggag tgaatatgag cgtattacgt tcgttattaa
     6001 ctgctggggt gctggcgtcg ggcctgttgt ggagcctgaa cggaattacc gctacccctg
     6061 cggcgcaggc atctggtgat cgctacgaag ttacccagca gcgtaacccg gatgccgcct
     6121 gtctggactg tcataaacca gataccgaag gtatgcatgg caaacatgct tccgttatca
     6181 acccgaataa caaattgccg gtcacctgca ccaactgtca cggccagcca tcaccgcaac
     6241 accgcgaagg ggtgaaagat gtgatgcgct ttaacgagcc gatgtacaag gtcggagagc
     6301 agaacagcgt ctgtatgtcc tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc
     6361 acgatgtcca cgtaaccaaa gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag
     6421 atacgatgca gacgttaagt gacaaaggac ggattaagat ttgcgtcgat tgccacagcg
     6481 atcagcgcac caatccgaac tttaacccgg cgtctgttcc gttgcttaag gagcagccat
     6541 gacctggtct cgtcgccagt ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac
     6601 cgcagggcgc gtggtggcga aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca
     6661 tgacgagtcg ttatgcatcg gctgtaccgc ctgtatggat gcttgtcgag aagtgaacaa
     6721 ggtgccggaa ggcgtctcgc gcctgacgat tattcgtagc gagccgcagg gcgaatttcc
     6781 tgatgtgaaa tatcgcttct tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt
     6841 tgacgtctgc ccgaccggtg cgtcttttcg tgatgccgct agcgggattg tcgatgttaa
     6901 tcctgatctc tgcgtcggtt gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt
     6961 tatccatccg gtcacgaaga cggcggacaa atgcgatttc tgccgtaaaa ccaatttgca
     7021 ggccggtaag ctgcccgcgt gcgttgaggc ttgcccgacg aaggcgctga cgtttggcaa
     7081 tctggacgat cctaacagtg agatttcgca actgctgcgc cagaagccta cttaccgcta
     7141 caagctggcg ctgggaacca aaccgaagct gtaccgcgta ccgtttaaat acggggaggt
     7201 gagccaatga cgcagacttc cgcatttcat tttgaatcgc tggtgtggga ctggccgatt
     7261 gccatctacc tgtttttgat tggtatttct gccggtctgg tgacgctggc cgtgctgtta
     7321 cgtcgcttct acccgcaggc gggcggtgca gacagtacgt tgctgcgcac cacgctgatt
     7381 gtcgggccgg gcgcggtgat cctcggtctg ttgatcctcg tcttccacct gacaagaccg
     7441 tggaccttct ggaagctgat gttccactac agttttacct cggtgatgtc gatgggggtg
     7501 atgctgtttc agctttacat ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat
     7561 gatttgcttg cccagcaaca acgctggttg ccgaagctgg ggatcgtgca aaaggttctg
     7621 agcctgctga cgcccgttca tcgcggactg gaaacgctga tgctggtgtt ggcggtgctg
     7681 ctgggggctt ataccggctt tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac
     7741 ccgatcctgc cggtgctgtt cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg
     7801 atcgccatgg cgatacgcca acgcagtaac ccgcattcca cggaagcgca ctttgttcac
     7861 cggatggaaa tccccgtggt atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt
     7921 ctggcgctgg gcgatgacgg taaagttcgt gcgctggtgg cggcattagg tggcggtttc
     7981 tggacgtggt ggttctggct tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc
     8041 aaatcgtggg tcaatcgcag ttccggcatt cctgccgtgc tggcggcgtg tggggccagt
     8101 ctggtcggcg tgttgatgct gcgctttttc attctctacg ccggacagtt gacggtggcg
     8161 taagccagaa aagaggtggt ttctggacgt attccttcct gaagtcggtt ttctggcgtt
     8221 gttgttaagt ctcggggtca acgtgttgac cccgttgacg gcctttgcgg gagtgcggtt
     8281 gcgctggcct gccatgatgc gactcacttg catcggcatt ctggcgcagt gcgcgctcct
     8341 gctgctcgcc tttggcgtac tgacgtattg ttttctcatc agcgatttct cggtcattta
     8401 tgtcgcccaa catagctaca gcctgctgtc gtgggaactc aaactggcgg cggtgtgggg
     8461 cggtcatgaa ggttcgctgc tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt
     8521 tgcctggcat tatcggcagc aaaccgatcc gctatttccg ctgacgctag ccgttttatc
     8581 tctcatgctc gccgcactgc tactgtttgt ggtgctgtgg tccgatccct tcgtgcggat
     8641 atttccacca gcaatcgaag gccgcgatct caatccgatg ctgcaacatc ccggtcttat
     8701 ctttcatcca ccgctgcttt accttggcta tggcggtttg atggtagcgg cgagcgtggc
     8761 gctggcgagt ctactgcgcg gcgagtttga tgcggccagc gcccgaattt gctggcgctg
     8821 ggcgttacct ggctggagtg cattaacggc ggggatcatc ctcggttcct ggtgggccta
     8881 ctgcgaactc ggctggggcg gctggtggtt ctgggatccg gtggaaaatg cctctttatt
     8941 accctggctt tctgccactg cgctgctgca cagtttatcc ctgacacgcc agcgggggat
     9001 ttttcgccac tggtcgctgt tactggcgat agttactctg atgctgtcgc tgctgggcac
     9061 cttaattgtc cgttctggca ttctggtttc ggttcatgcg ttcgcgctgg ataacgtccg
     9121 cgccgtgccg ttgttcagcc tgtttgcact gattagcctt gcgtctctgg ctctgtatgg
     9181 ctggcgagcg cgggacggtg gcccggcggt gcgtttttcg gggttatcgc gggaaatgtt
     9241 aatcctcgct acgctgttgc tgttttgcgc agtgctactg atcgtgctgg tgggaacgct
     9301 ttatccgatg atttacggtc tgctgggctg gggacgcctc tccgttggcg cgccgtattt
     9361 taaccgcgcg acgttaccgt ttggtctgtt gatgctggtg gtgattgtgc tggcgacgtt
     9421 tgtctctggc aaacgcgtgc agcttccggc gctggtagct catgcgggcg tgctgttatt
     9481 tgccgctggg atcgtggttt ccagcgtcag ccgtcaggag atcagcctga atttacagcc
     9541 gggtcagcag gtgacgctgg caggatacac cttccgtttt gagcgcctcg atctgcaagc
     9601 caaaggcaat tacaccagcg aaaaagcgat agtggcactg tttgaccatc agcaacgcat
     9661 tggtgaactg atgccggagc ggcgttttta cgaagcacgt cgtcagcaaa tgatggaacc
     9721 gtcaattcgc tggaacggca tccatgactg gtatgtggtc atgggtgaaa aaaccggagc
     9781 ggatcgttac gcttttcgct tgtatgtaca aagcggtgtg cgctggatct gggggggagg
     9841 attgttgatg attgcgggcg cattgttaag cggatggcgg gggaggaagc gcgatgaata
     9901 aaggacttct cacgctactg ctgttattta cctgttttgc gcgcgctcag gtcgtagaca
     9961 cctggcaatt cgccaatccg caacaacagc aacaggcgtt aaatattgcc agccagttac
    10021 gttgtccgca gtgccagaat caaaacttac tggaatccaa cgcgccggtg gcagtcagta
    10081 tgcgccatca ggtttacagc atggtggcgg aggggaagaa cgaagtcgaa atcattggct
    10141 ggatgaccga acgctacgga gattttgttc gctataaccc accgttaacg ggtcagacgc
    10201 tggtgttgtg ggcgctgcca gtggtgttgt tactgctgat ggcactgatc ctctggcgag
    10261 tgagggcgaa gcgatgaaag agcccaaaat accggtgaaa atgctgacaa cactcacgat
    10321 tttgatggta tttctctgtg tcggcagtta tctgttaagt ccaaaatggc aggcggtaca
    10381 tgcggaatat cagcgtcagc gcgatccgct acatcagttt gccagccagc aaaccccgga
    10441 agcgcagctt caggcattgc aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg
    10501 ggcgttactg ggcgagtact atctgtggca aaacgattac agcaattcgc tgctggcgta
    10561 ccgtcaggcg ttgcaactac gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt
    10621 gctttattac caggccagcc agcatatgac cgcccagact cgcgcaatga tcgacaaagc
    10681 cctcgcgctg gactctaacg aaatcaccgc tctgatgctg ctggcttccg atgcgtttat
    10741 gcaggcgaat tacgcgcaag ccatcgaact atggcaaaaa gtgatggatc tcaactcacc
    10801 ccggatcaac cggacacagc tggtcgagtc gattaatatg gcgaaattgt tgcagcggag
    10861 atcggattaa tcatctggtg atgaagatta tgttattgag ctgcataaaa aataatcgaa
    10921 tgaacatatg ccaaaaataa tcactaatca gtattattgc agattaaaca aataaaaatc
    10981 tttccataac aaatggttat tcattaatcc tgacataaac tcgcgttatt atgcattaat
    11041 gcagcgaaaa gctctgttgt taaagggttg cgcaacatac cgcgcaaatg atactgatca
    11101 taagcgttaa aaaaatctac aaaccaacgc aacacaattc atgcctggca gtatgtcacg
    11161 ttctcgcgtt tctgaacggg gaacggcgct ccattgagga agttattctg atgaaaaata
    11221 taaaattcag cctggcctgg cagattctgt ttgctatggt gctgggcatt ctcctgggaa
    11281 gctacctgca ctaccatagc gacagccgcg actggctggt cgtcaatttg ctctctccgg
    11341 cgggtgatat cttcatccat ctgattaaaa tgattgttgt gccgattgtg atctccacgc
    11401 tggtggtggg tatcgcgggt gtgggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa
    11461 ccattattta cttcgaggtg atcaccaccg tcgccatcat tttggggatc actctggcga
    11521 acgtcttcca gcccggtgcc ggggtggata tgtcgcagct ggcgaccgtc gatatctcga
    11581 aatatcagag cactacggaa gcggtacaaa gcagttccca cggcattatg ggcacgattt
    11641 tgtcgctggt gccgacgaac attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca
    11701 tcttcttctc ggtgctgttt ggtctggggc tttcctccct gcccgcgacg catcgtgaac
    11761 cgctggtgac cgtgttccgc tccatctctg aaaccatgtt taaagtgact cacatggtga
    11821 tgcgttatgc accggtgggt gtgtttgcgc tgattgcggt gacggtggct aactttggtt
    11881 tctcttctct gtggccactg gcgaaactgg tgctgctggt gcatttcgcc attctgttct
    11941 tcgcgctggt agtgctggga attgtggcgc gcctgtgcgg gttaagcgtc tggatcctga
    12001 ttcgtattct gaaagatgag ctgattctgg cgtactccac tgccagctct gaaagcgtac
    12061 tgccgcgaat tattgagaag atggaagcct acggagcacc ggcgtcgatc accagcttcg
    12121 tggtgccgac cggttactct tttaaccttg atggttcgac gctgtatcaa agtattgccg
    12181 ctattttcat cgctcagctg tatggcattg acctgtccat ctggcaggaa atcattctgg
    12241 tgctgacgct gatggtgacc tcgaagggga ttgctggcgt gccgggcgtg tcgtttgtgg
    12301 tgttgctggc aacgctgggt agcgtaggta ttccgctgga aggtctggcg tttattgctg
    12361 gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg
    12421 cggtgctggt gattgccaag tgggaacaca aatttgaccg caaaaaggcg ctggcttatg
    12481 agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca ataattgaag attgccgggg
    12541 ttatccaccc ggcaatgtgt ggatgcctga tgcgacgctg gtgcgtctta tcaggcctac
    12601 gccagacagc gcaatagcct gatttagcgt gattttgtag gtcggataag gcgtttacgc
    12661 cgcatccgac atcaacgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccttaacc
    12721 gttggttaat ttttcaaact cttcacaccc ggtatcaaac ccttccatac agctcaggtt
    12781 caaccagtgc agcgcctttt gtttgttctt ctcgataaaa ccttcttcac cgtttaagaa
    12841 catcattccc gcccagtact cggaataacc ggttcgggaa attgcagagc tgcgtttgaa
    12901 atactaggtt gccttgtcat cgacggcaga gtcgttctcc agatcttccg aggcttttct
    12961 aacagcgaaa tcgcctttgg ataatccggt ttacccgctt gagtatttac cagaatatgc
    13021 gccagcgtta cttcaccttc tttactgccc gcttgcgccg ctttctctgc aagaactttt
    13081 gcttgtggat agtccagact gcccggattg gtgattttga tctgcgccaa cagcgcgcag
    13141 gcatcagcat caccgctgtc ggcggctttc tgtgcccaat attcggcttt gctcaaatcg
    13201 ccggagctaa accagctgtc ggcaagaaaa tattgtgcgc gtcggtcccc ggcctctgct
    13261 gcctttaaat actggctgcc aggctcggag tcgttggcgt gggcaaagaa tgtcaaaaac
    13321 aacatcaatg cgataatttt tttcattttg ataatacgat tgggtaggga gtaaccagta
    13381 taaagagttc gaatggataa aaaaacagcc tccgaaagga ggctgtagaa aggactgtat
    13441 tacgccagtg ccgcttcgcg caggcgagtt ttcaacttgt tgtactcgtc aatcacgtac
    13501 tgctcggcgg cgcgctgatc ggcgatcggc tcgacgcgca cggcgcagta tttgtattcc
    13561 ggcgttttcg taatcgggct taagttttcg gtaaccagct cgttacagga accaatccac
    13621 cactggtagg tcatgtaaat cgcccctttg ttcggacgat cgctgacctg cgcacgggtg
    13681 ataattttgc ctttacgcga gtgcacccaa accaatgcct catcttcaat acccagacgt
    13741 ttggcgtctt cggtattgat ttgtgcgtag ccaggttcat cagccagcgc cgccagtgcc
    13801 gcacagttac cggtcatcga acggcaagag tagtgaccaa cttcacgcac cgttgacagt
    13861 accatcgggt actcgtcggt gagtttgtcg attggcgcta cccagtcgca ggtgaagaac
    13921 tgcgccagac cgttcggggt atcaaacttc tctttaaaca gataagaagt cccctgatcg
    13981 gcatctgaag tatcgcggca aggccactga atgaagccct gttcgcccat tttctcgtaa
    14041 gtcgcaccgt agaaatccgg gcacagatga cgcaactcat cccagatctc ctgggtgttg
    14101 ttgtagtgca tcggataacc catacgggtg gcgatttcac tgatgatttg ccagtccgtt
    14161 ttcagatccc atttcggttc aactgctttg aagaaacgct ggaagccacg gtcagccgca
    14221 gtaaacacgc cttcatgctc gccccacgac gttgacggca aaatgacatc cgccgccgac
    14281 gcggttttgg tcataaagat gtcctgaacg atgaccagtt ccagatcttc aaaggcttta
    14341 cgtactgccg acagctccgc atcagtttgc agcggatctt cgcccataat gtacgcggca
    14401 cgcacttcgc catgcgctgc gcggtgcggc agctcgctga tgcgatagcc ggtatgagca
    14461 ggcaggcttt ccacgcccca ggctttggcg aatttctcgc ggttagccgg atctttcacg
    14521 tactggtatc ccggatacgt atccggcagc gcgcccatat cgcaggcacc ctgcacgttg
    14581 ttctgaccac gaaccgggtt tacacccgca tgcggcttac cgaggttacc ggtcagcatc
    14641 gcgaggctgg tcagagaacg cacggtttcc acgccctggt agaactgggt tacacccatg
    14701 ccccacagga tggcggcgct tttcgcctgg gcatacatcc gtgccgcctg acgaatctca
    14761 ctggcgctga cgccggtgat atcttcaacc gactccggcg tgtagccttc aacgatttta
    14821 cgatactctt caaagccttc tgtacgggaa gcgacgaacg ctttgtcgta cagattttct
    14881 tcaataatga catggcccat cgcattcaac agcgcgatgt tcgagccgtt tttcagtgca
    14941 atgtgcatgt cagcaatgcg cgcggtttca attttgcgcg gatcgcagac gataattttc
    15001 gccccgttac gtttagcgtt aattacgtga ttcgccatga ttgggtggga atccgccggg
    15061 ttgtacccga aaacgaacac taaatcggta ttatcaattt cgttaatagc attgctcatt
    15121 gcgccattac cgaccgattg gtgcagacct gcaaccgatg ggccgtgtca gacacgagcg
    15181 cagcagtcaa cgttattggt accaataacg gcgcgcgcaa atttttgcat tacatagttg
    15241 gtttcgttac ccgtaccacg cgaggagccg gtcgtctgga tggcatccgg accgtacttc
    15301 tctttgatgg cgctcaggcg ctcggcaacg taattcagtg cctcatccca ggaaacaggt
    15361 tcgagtttgc cgccacgctg gcgacggatc atgggggttt tcaggcgcgg ggtcaggatc
    15421 tgggtatcgt taatgaagtc ccagccataa taacccttca gacacagggt accctggttg
    15481 gttttcccct gcgctgcctc cgcccggacg attttgccgt tatcgacgac caggttgatt
    15541 ttgcaacctg atgcgcaata ggggcaaacc gtgacgactt ttttcatcgg tctcgctcca
    15601 gttaatcaaa tcacgcatac gcgctctcga ctacagtatg catcttttat gccacatttt
    15661 atttggggtc attccctgat attacgggca ctatttattc aaaactctga cgaaaaacag
    15721 gctgtcgtca gttttgacgt gacgaaacga aataccgcgt gacagccatc acgcggcaga
    15781 cattttattt tttctcgacg acgggagccg cctgataccc gccgcccagc gatttcatca
    15841 actgaatgct gtggatcacc cggcggctgt ccagcatcag taatgacatc tcttcggcaa
    15901 gcactggcaa ccgggcttcg gtggcctgta agcggctggt taagccgcgc tgataggcgg
    15961 cctcggcagc gcgctgggtg aagcgcgttg cttccacgcg ttcagcctgc atttctcgtt
    16021 cgtcgttgag cgtttgcaga cgcgtgccgt tgacggcaac gtcacgcacc gcgttcagta
    16081 ctgactggtt gtaacgttca atcatcatgt tgctggcggc gcgagtgcct tcgagattgg
    16141 cattcaaccg tccaccgtca aacagcggca atttcagacc cgggatgaag ttgaactggc
    16201 gactggtttt tttgaataag gtatccaggt ggatggagtc cagaccgaaa aacgctttga
    16261 tatcaaagct cgggtagaac agtgcccgcg cggaatccac ctgatctaat gacgcctgaa
    16321 cataccagcg catggcttgc agatccgggc gtctggcgag caactcataa gagagtgtcg
    16381 ccggaatgcc ggtctggact cgtggtaatg ccaccggttt gatctccggc atatcgctgg
    16441 cccccgcgcc aatcaatgca cgcagagatt ctcgcgtttc ggtgatttgc cctttgacgg
    16501 cagcaatttg tttatcgacc gccagaatct gtgcccgcgc gccgtggaaa ggcacttgtg
    16561 cttccagacc gtgcgccact ttactctggt gcgctttcac cgcgtaatca atcacatcgc
    16621 gagtttgttc taacagatcg agcatctgat agctggcctg catactgtaa taaagctgcg
    16681 ctacgcccgt ggtcagcgat agctctactg ctgcggtttc tgccagcgcg gcattatgcg
    16741 cgccaatggc ggcggcaacc gctgagcgat gcacacccca caaatcaaga tccagtccgg
    16801 caaacaaacc tactgtggct tccgtatagt acggcccgtc catacccagt gagggcgcat
    16861 ccatcgcata agggcttaaa aagccgttcg ccgagacgcg ttggcggttg agcatcccta
    16921 acgctgcgac ctgtaattgt gaaccggcat ctaacaaatc ggcctgggac tgcgcttttt
    16981 cttcccgcag tttcgcttcg gcgagggtgt gtgaaccact tagcgtccgt tggatcagcg
    17041 catccagctg cgggtcattg agttgtttcc accactgcgc ctgcggccag ccggagctgg
    17101 caagatgaat atcgtcggcc agtttgattt gttccggttt gagctgttga tgaggtgcag
    17161 aatccttacg taccagggca cagccggaaa tcagcgtcgt gctgccgaga atgctgcaca
    17221 acagcagacg tgaaagttga cgattgatca ttgcgtggct ccttgtgcct gttccgtggc
    17281 gggcgttaat gccggggctg tccagtctgg caagcgggcg aaaagctggc agacgtgttg
    17341 ctcctgtttt aataaggtgg gcaggatggc ctgcgaggcg ggtgtctctt caagcgttat
    17401 ttgaggagaa cagctgagtg cggttgccag accggcagcg tatttttcca gggcgtcggc
    17461 aaactgacct gctctgtccg gctgtagtgc gttatccagc gcctgcgccg agttccaggt
    17521 ggcatcccag gcgtgaagaa gatcgcggcc ctgacgaata accgtttgcg aacgttcaat
    17581 cagtaaggcg cgttcttcgc tgtccagttg acgctccagc gccacgcgtt ggcacatctc
    17641 ttcacaggca ttaaacgccg catgcagacc tatacgaatt tgcagataag tgcgcagagc
    17701 cgtgacttcc tgctggcgtg ggatccgcat tactttactt agcataccca gcgcaccggc
    17761 tagtttttgc ggcagagtgc gcgcttcact ttcaggccag acaaaggtgt aaatcaccgc
    17821 ggacaccacg gtaccaatga tgatacccag ggcgcgatcg cgaatttcca ccaggtcgta
    17881 cactgggcca aaaacgtttt cgagcgtggc gagcgcgaag gtgacgacca tctgtgtgcc
    17941 gatataagaa gagcgttcag agctggtggc aatccatgcg cccaacagga aaatcggtgc
    18001 cagcacaaac agcaattcga caatattgtc cagccagggc atgaccagta gcgtgaataa
    18061 cagcgccaga atcgcgccgc aaaaggcccc gccaaaacgc agcaccatct tctggtacga
    18121 cgaaccgaca tttggattag cgacgatcac gcaggtcagc atacaggtgt gaatgccttc
    18181 ccagtccacg ccgctgtaaa aggtgtaaca gatcaaacag gcgagcaatg tttttaccgc
    18241 gtagcgtata tagtctggat tggtaaaagc atcggcgacc attgatggcg gtttggcggc
    18301 gggcgttggc ggcgtattcg ggttcatctg gcccagttgt aacaacgtct ggcagatatt
    18361 ctccaggtta cattcccgtg ccgccaccgc ttcactttca ctgatccgcc agtcgctttg
    18421 ccagcactga ccttcagcaa cggcatgctg cagcttgttg atttctgagg ctaatttttg
    18481 ccgaaattca ataattgcct gagaatcagc aaaagaggtg ggatcgtagc gattcagcgt
    18541 cgagtaaatg taggttaccg ttgccacgca gctttgccac catgcgcttt gagttcgcca
    18601 gttggcatcg tccgcgagac aaaagacatt cagtttttgc agcgccagcg cctctctttc
    18661 aatccgcgtt tcgggtagcg gtgcgaggct gtctgtcagg tgactaatgg catcatcaag
    18721 ccgatcatta agcgcctgat gcatttgcga aatggcacga ctgggaaacc acagcacgcc
    18781 gattaacgtc atcagcaagg ttggatagag gccaacaacg atacaccaca gcgttaagcg
    18841 cacgaccact tccggatagt cgagcatggc ggggaaggtt tgcccgtaaa tggcgacaat
    18901 ggcgacagcg aaaaagacca gtcccagccg atgggtgcgc atcaaaaaca tgcagcccat
    18961 caggatcggc ccggcgatga tcaatcggat caacggttcg ccgtatgacc atttgtagat
    19021 caaaaacagg ctgccgatct ccagcaccgt ggcaaccaca aacaagatcg ccacaaattt
    19081 ggtgtaaaac gcgttcgact gaataccgta aaacagcact gccagcgata acgccacaaa
    19141 agggatctca aaagtcatcg agatcagaat caccagcagg cagcctaccc agagttgcac
    19201 gatctgcggc acgcggccgg gtcgccgctc gcttaactct tcatgaaaga acgccagcag
    19261 cctgaccacc ggtaatggca gggagttgag cgcgctcatt attgcggctc aagattagcg
    19321 actgccgaag cgccgatgcg gaacatttcc gggtcaggtt tatcgaccat gattttgacc
    19381 ggaaaacgct gggcaacgcg gacccagtta atagagcgag aaactttcgg caggccgccc
    19441 agcaccaggc cgccgtcatt cggtagcaca ccgtagccaa tcgaatccac tttaccctcg
    19501 aaggttttgc cgctgtcact catcaggcga atcgttgcgg gtgtacctga gcgaatattt
    19561 ttcagatcgg tttcgcggaa gttggcgatc acgtaccagt gacgagtgtc gattagggta
    19621 aaaatagggc gcatggcaga agcaaattgc ccgacggagg ttttgaggga aatgacccgg
    19681 ccatcaaacg gcgcgcgaac ggtcgccatt tccagatgca gtttggtcag ggcaatatcc
    19741 gcttcgaccg ccgcacgctg ggcaactaat acatccacgc cgctgacggc gctggcggct
    19801 gactgcgcct gtaacaatac ggcattaaga tccgcttctg cggcgcgctg cgccgttctt
    19861 gcacggtcaa catcttccgc tgagacaaaa ccttctttca gtaatggctc ggtgcggcgt
    19921 aatgtatctg tggcctgttt cgcggcggca cgtgcttttt ctaccgtggc attaaccgag
    19981 tcggcaccaa actgttgggc gtcaacgcta cgctgggtga gcataatttg cttatccagc
    20041 gccgcgaggg aggcttcagc tttcgccaga ttggcttcgt acgggcgcgg gtcaatgcgg
    20101 aacagcaaat cgccctgttt gactgcctgg ttgtcggtga ccgccagttc tacaatgcgg
    20161 ccgctgactt ccggcaccac atcaatggta tctgctgacg cgtaagcgtc attagttgat
    20221 ggcgcactgt ctacgcgcca gataacgaaa acaagggcaa ccagcgccaa cgcaaccact
    20281 aacagagcag ggaatttact gcgaggagct tttttcggcg tactttccat gagcgcgtct
    20341 caaatagatt agaaaaatgc cagccagaac agcatggcgt agacagcaaa aagggcggta
    20401 taaataattc cgacaaatgc cagattgata tttgcccgct gaataattcg cctcgtgatt
    20461 agcgtcaaaa taaggctggc aatggcgcag aaaaaccagc tgggataata agcgccgatc
    20521 accgggattg ccggagataa tgagcatccc gaaaccatga agacgggaat tatccaggct
    20581 gtttttttga gttgcatcgc catgcgagag agaactgttg gcatattaaa tacatcctgt
    20641 ttgctgcgaa caatatctgt agtgaatatc aatatccatt gaaggcgtca gtatggctat
    20701 tttcttgatg aataaaaata gctttattca gccaccgcat tcataactta tatattttat
    20761 gtgttaattg attgtatgta tttttataat aaatgctttt gggggcttaa gcgcccccaa
    20821 aagtgagtgg cattaatttg gggttacgat attcacccac aaatcgaaat tatccagaca
    20881 ggcaataatt tcttccagtt tcgcgccatt gccaataatt ttggctttct tcgcttttac
    20941 cagatccgcc attttggctt gtcctgtcag cacggcgtgc agatcttcac ggctgatgta
    21001 gaaagaggcg tcggcttgcg gttgcagtgt tttacggtag ttaagcacgc tatcgttcag
    21061 cgtcaggttg aggttatcgc cgttgctcat attgaagttc aggctgatat ttttacccgc
    21121 agctttcgcg ctatcgaggc gaacggacat aaagtcgaac agcatttcga ccgacatccc
    21181 gcgaatggtg tccggggaac cggtggtgcc gtggctgaac ttatgcaccc cttcgcgcag
    21241 ctctttcgcg ccggtcaggt agaaaccgcg ccaggtggcg gactcggcct gatagcccag
    21301 ctgttcaaag ttattcgctt gcagattctt cgcgacctgg tcacccgggt tggcggcaat
    21361 cacctgtttc agcagttctg ccgaccagcg gtaatcacct tgcttgttcg cttcttgcgc
    21421 caggctgatg acacgggcag aaccgcccag cgcctgcacg taacgtttac ccatctccac
    21481 ctgaccatac ggatgcaggt tagccgggtt accgtcgtaa tagccaagat agaagttata
    21541 caccgcgcgg gcgttgtggc tgacagaacc gtaatagcca cggctggccc agttattggc
    21601 aagtgcaggc ggcagtttaa tcatgtcgcc gatttcattc atggtgtagc cctggttcgc
    21661 caggtgcagg gtctggtcgt gaatgtactt gatagtatcg cgatatttac caatataatc
    21721 attgatatgc ttattgcccc ggaccggcca ggtgtgcggc ataaacagga cttccgcgtc
    21781 gttaccccac atatccagcg tttcgttcag atactcggtc cacttgctgg tgtcgcgggt
    21841 tttcgcgccg cgcagagtgt agaagttgtg cagggtatgc gtggcgttct cggcggtaca
    21901 cagcgctttc agggccggaa tatagaagtg catttcggct ggtgcttcgc tacctggggt
    21961 catcaggaag tcaaactcca ggccgtcgat aatcatcttc tcaccggttt tggtgatggt
    22021 tttgttcggt gcgatgatgc tcgggtagcc cgatgccagc gttacgccaa gaccattgcc
    22081 gatgttaccc tgagggttat gcgccagcag caggccgtag gagtaaagtg cacggtggct
    22141 cataatatta cccgccagta cgttttcgct gatggcttcg tccataaagc cagcaggggc
    22201 gataacctgc actttaccgg atttcacatc agcttcagag ataatgcctt tcacgccgcc
    22261 atagtggtcg gtatggctgt gggtataaat aaccgcaaca atcggttttt gcgggcgatt
    22321 ttggaagtaa aggtcaaggg cggcttttgc gctcggtggc gtcaccagcg ggtcgatgac
    22381 aataatcccg gtatcacctt cgataaaggt gatgttggag atatcctgac cgcgaacctg
    22441 gtacattctg tcggtgactt taaacaggcc ggaaataccg tttaactgcg actgacgcca
    22501 caggctaggg ttaacggttt cgggcgcggg cgcattgata tcaaatttat aatcttcccc
    22561 gcgataatat ggtttgccat tagcatcgcg cagaatacct tcatccagca gcggagcgat
    22621 aaatccacgc tgagcgtcgt caaaatcctg acgatcgcta aacggtaatg atttggcgta
    22681 attctgatta acctgttggg tatattgagt ggcggctttc gcttcctctt tcgcgttagc
    22741 ggtatttacc atcatgccgc tggcggcagt taacgcaata acaattctgc tcaaacggaa
    22801 taaccgagag ttattcatat aaatctccat ttatcattta tgtcgtaaat atgtaatcca
    22861 tacaaaatac gtcatttaaa gctggggtta tcataattgg gaatttgcga ctttaataag
    22921 tggaagtgtg agctgaacga tccattttat taggcatttg tgctgaagag agaaaattac
    22981 gcgaatttat tatctctgta aataatatga ggtgttttat atatttatta tatatattgt
    23041 acttcggcaa atactgagca caatttatca atgcaaccgg tgtttctttt taaaaaacgt
    23101 accggagcaa tgggcggtca tgtcgctggc accgcaggat tctgcgttgc tgtgccagcc
    23161 ttttattcat ggtcaatgag tgaggtgatt gataaagaca ccaggtcgtc agagcgcact
    23221 gattatccgc attatgccag tgaagttgca ctggcttttg gttcacgagt ggttggcctc
    23281 gaactggcaa aaatgattgt ggatgcgtgg ctgggcgcac agtacgaagg cggtcgtcat
    23341 caacaacgcg tggaggcgat tacggcaata gagcagcgga gaaattgaga ttcatccact
    23401 acttgcatgg atgagtaatg attaatgtgg atagagtttc tttttgaggt tggctaatga
    23461 aacgctttcc gctatttctt ttattcaccc tgctcacgct gtccaccgtt ccggcccagg
    23521 ccgatattat cgacgacacc attggcaata ttcagcaggc gattaacgac gcttataacc
    23581 ctgaccgtgg gcgagactat gaagattcgc gcgacgacgg ctggcagcgt gaagtgagcg
    23641 acgatcggcg cagacaatat gacgaccgcc gccgccagtt tgaagatcgc cgcaggcagc
    23701 tggacgatcg ccagcgccag cttgatcagg agcgtcggca gctagaagat gaagagcgga
    23761 gaatggaaga tgagtatggg cgatgaggtt gggtatggag caggcatcgc ccctcacccc
    23821 aatggggaga gggagaaaac cagcgcaata ttcaatatcg agcacaatcg gtcccctcgc
    23881 ccctctgggg agagggttag ggtgaggggg ccagcgcgca cattccctct catcacgcca
    23941 ccccaatctc catcccatca aatcccgcct caaaccccga cggcagtgcg ttttccatca
    24001 gccacgcatc aaactggtgg ctgatatggg tcagaatcac ccgtggcgag cggataacct
    24061 gattcagcgc cagcacggtg tttaaatcac agtgactacg tggcgcatcc tcgcgcggcg
    24121 ggtggctgca atcaatcacc attacctgcg gctgattatt gcgtaaaaat ttcagcgttt
    24181 tttccggcaa gcctgcggtg tcagacagcc acgccacccg gctgtgtgcc gtttccagca
    24241 gataaccgaa ggtcagtttt gagtggttga gcggcagggg cgtgacctgt aacccctgca
    24301 aatcaaacac cacaaacggt tccaccgtgt ggctgaaatc aagcaggccc ggatgtttaa
    24361 acagatcgtc gcagccctgt tcatccggcg ggccgtaaac cgggatcgga tcgcccacgc
    24421 cccagcgcag cgggaatagc ccctgcacgt gatccatatg gtagtgcgtc agcaaaaact
    24481 gctggaacga tccgggcggc cagcgatcgg cgagatcgtg cagcccggcg tcgatcaggg
    24541 tgattgcgtc gttaaacttc actacgccgc tgcatggctg gcggcgatac tgcggtgagc
    24601 gccgcgctct ggcgcaggct acgcactcgc agccccatgc cggaacgccc tgtgcgccgc
    24661 cggtgccggt gagcgtgagg gtcaggctca tgttacagcg ccttggtgaa gcggaagtgg
    24721 ctctgctcgt agccttcacg caggtaaaaa cggtgcgcgt cgtggcgctt cacgttggtg
    24781 gaaagttcgg tcatttcggc cccggcctgg cgggcttctt cttctgccca cgccagtaac
    24841 ttactgccga cgttcagacc gcacgcctgc ggcatcacca ccagttcctg aatttcgccg
    24901 atccagttga catgatgcag atgaaactgc aaatgcaggc cgatcatgcc gacaacttcg
    24961 ccatccagca gcgccagatg gtagcgcatg ctcgggtagc gcatgctcgg gtagcgcatg
    25021 ctcgggtcgc gcaggttggc gttaaagccc acgcgaaacg cgtggtggtc aaactccgcc
    25081 tgttttagct cacaaatcag cgcgtaaacc gcgtcggtat cgtactgcgt ggccgggcga
    25141 agctcacaag caggcatggt gtttctcctt ctgatgaatc agcgtcagca gtgtgtcgac
    25201 cgactggcgc aggctgccgt cattgttgag cgtatggcaa tcctgtggag tatagcgggc
    25261 ggcgcgcgcc agccgggcgt taatttcact ggtgttttca cggccacggt ttttcaggcg
    25321 ctggcgcagg atctccggcg aaacctgtaa acagacgggc agcagcgccg attgatagcg
    25381 cgcccgtgcc tgcggcaaat gcgcgcgtga accgttgacg actacgtcga atccagcgtg
    25441 cagccagagg tcaatctcga tgccgacgcc ataatacaga ccgttagcgt gccagcttaa
    25501 ggccagcagg ttttgccccg cgcgggtaaa aaactcccgc tcactcaggg cgatatggtt
    25561 ttcacttccg gcgctggcgt cgcgcgtgat gtagcgatgc gccaccagta actgcgtttg
    25621 ttcccgcagg cggagttccg ccagcaggct gtctttcccg gagccggacg gccccattaa
    25681 ccaaatcagt tttcccatca tcagaacacc cttttaccct gacgccagac gtggtcgata
    25741 tgaatgtgat tgccctggcg atgcgccagc accaggtcag cgcgtttacc ctcgccaatc
    25801 accccgcgat cctgaagatt cagcgcctgc gccggatttt tggtcaccag cctcaccgcc
    25861 tgcggtagcg taaagcggtt gctctcgtca tcggcgacgc ggaacgccgc atccagcagg
    25921 ctggcggggt agtagtcgga agagaggata tccagcagac caagctgcgc cagttcactg
    25981 gccgccacat tgccggagtg cgagccgcca cgcacgatat ttggcgcacc catcagcacg
    26041 ttcatgccat gcttacgcga ggcttccgcc gcttcgaacg tggtggtaaa ttcggcgatc
    26101 acgctgccaa gttggtggga ttcggcaacg tgggcgtgcg tggcgtcatc gtggctggcg
    26161 agcgcaattt gtcgcgcgcg gcacagggcg gcgatggatt cgcgattcgg ctgcgaccag
    26221 cgtgcggcga gcgccaattg ctcttcttcg tactgctgca tctgcgcatc ggtgagggag
    26281 tatttgccct gataatattc gcgatacttc tcgcggttag cgaactggcg ctggcccggt
    26341 gagtggtcca ttagcgacac cagcgtcacc ggttcgcgct gaaccagttt ttcaaacagc
    26401 ggcagtgtgg tgtgatgcgg cagctcgcag cgcaggtgca gacggtgctc ggcgcggttg
    26461 acgccgcgtt tctgcgtctc ttcgatggtg ttgatcatct tctccagatt ctccagctga
    26521 tcgccgccgt cgcgcacgtc gccaatcgcc accgcgttca gcacggtggt gatgccgctt
    26581 gccaccatca gcgcgtcgtg gctgctcatc gccgaatggg cgggccagtc gactttcggg
    26641 cgcggggtga agaatttatc gaggttgtcg gtatgcagct caatcaggcc gggtagtagc
    26701 cagccacctt cgccgtccat cgcttctggc aggcggctct ggctttcggc aaaggcgcgg
    26761 atttcgccat cctgcacctc cagcgaaccg tgcaccacct cgttttccag caccagctta
    26821 acgttattga taatcatgaa gaggctccca ttgggtgcag gcggtcggcg acgtcattac
    26881 gtacagcttc gtcatggaag atgccgacga tggctgcgcc acgggctttg gcttcgtgaa
    26941 tcagttccac caccgcggcg ctgtttttgg cgtccagcga ggcggtaggt tcgtcgagca
    27001 gcagaatggg gtagtcgacg ataaagccgc gggcgatgtt gacgcgctgc tgttcgccgc
    27061 cggagaaggt cgatggtgcc aggtgccaca ggcgttccgg cacgttcagg cgggtgagaa
    27121 gacgcgcggc tttagcggcg caggcttcac gcggaacgcc ggtatcgagc agcggctgca
    27181 tcaccacttc cagtgctgag atacgcggga tgacgcgcag aaactggctc acccagccga
    27241 ctgtggtttt gcggatttcc accactttgc gcgctggcgc ggtgaccagg tctacccact
    27301 cgtcaccgtg tttgatctgg atttgacctt cgtcgggtag atagttggcg tacagcgagc
    27361 gcagcagggt tgatttgccg ctgccggagt gaccgtggag caccacgcat tcgcctgcgt
    27421 tgacggtgag cgaggcacgt tgtaagacgg gcaggcgtac gccgttttgc tgatgcagga
    27481 tgaaggtttt actgacgttt tgtacgttaa tcatttttcg cctcgtggtt cgggtgtcgg
    27541 atgcgacgct gacgcgtctt atccgaccta cggggagcgc atttgtaggc cggataaggc
    27601 gtttacgccg catccggcac cggctcaatt ctgcaaaacc gatgacacca gcagttgtgt
    27661 atacggatgg tgcgggtcgt cgagcacgcg gtcggttaac ccactctcca ccacttgccc
    27721 ctgcttcatc accagcaaac ggtccgccag caggcgggcg acgcctaaat cgtgggtgac
    27781 aatcactacc gcgaggttca gctccaccac caggccgcgc agcaggtcga gcaggcgtgc
    27841 ctgtaccgac acatccagtc cgccggtcgg ctcgtccata aacaccagct tcggatgcgt
    27901 caccaggttg cgggcgatct gcaaacgttg ctgcatgccg ccggaaaagg tggtcggcag
    27961 gtcgtcgatc cggttggcgg gaatttccac ctcttccagc cacttctgcg cggtggcacg
    28021 aatatcgccg taatgacgtg ccccggtcgc catcagccgc tcgccgatat tgccgcctgc
    28081 cgacacctgg cggcgcaggc cgtcgagcgg atgctgatgc actacgcccc attcggtgcg
    28141 cagcaggcga cggcggtcgg cctcgctcat tgcgtacagc gaacggctct catagtgaat
    28201 ctccccctgc tgcggcgtca ggcgcgcgga gattgatttc agcagcgtgg tctttccgga
    28261 gccggactcc ccgacaatgc ccagcacttc ccccggccat aaatcaaaag agacatcgct
    28321 aaagcctttg cccggcgcgt aaaggtgggt caggttattg accgaaagta acggttgatt
    28381 cattggtttt ttgcctcgct ctgttggcgg caataatcgg tatcggagca gacaaacatg
    28441 cggtttccgg cgtcatccag caccacttca tcaagatagc tgtgggtcga tccgcagatg
    28501 gcgcatggct catcccactg ctgaacggtg aacgggtgat cgtcgaaatc gagactttcc
    28561 acgcgggtaa acggcggcac cgcatagatg cgcttctcgc gccccgcgcc gaacagttgc
    28621 agggcgggca tcatgtccat ttttgggtta tcgaatttcg ggatcggcga cgggtccatc
    28681 acgtagcgcc cgttcacctt caccggatag gcgtaggtag tggcgatatg accgaagcgg
    28741 gcgatatctt catacagttt cacctgcatc acgccgtact cttccagcgc gtgcatggtg
    28801 cgggtttccg tttcgcgcgg ctcgataaag cgcagcggtt cggggatcgg cacctggaag
    28861 ataatgatct gatcttcggt cagcggcgtt tcggggatgc gatggcgcgt ctggataagc
    28921 gtcgcatcgt ccgtacgttc agtggtgttt accccggtca cgcgcttaaa gaagttgcga
    28981 atcgacacgg cgttggtggt gtcgtctgcg ccctggtcaa tcaccttcag cacgtcgctt
    29041 tcgccaatca cgctggcggt gagctgaatg ccgccggttc cccagccgta gggcatcggc
    29101 atctcgcgcc cgccaaacgg cacctgataa ccggggatcg ccaccgcttt taagatggcg
    29161 cggcggatca tgcgtttggt ctgctcgtcg aggtaggcaa agttgtagcc gctcagatta
    29221 gccatggttc tgctcctgtt gcagacgttt gagtagctcc agttcggcct ggaaatcgac
    29281 gtagtggggg agtttgaggt gcgagacaaa gcctgcggct tcgacgttgt cggcatgtgc
    29341 cagcacgaac tcttcatcct gcgccgggcc tgtcgcgtgc tcgccgtact ccggagcctg
    29401 caacgcacgg tcaaccagcg ccatcgcttt gcgctcgctc atgccgaaca ccagcccgta
    29461 gccgcgcgtg aagtgcggcg gctcatccgg cgggtcaata aaaccgttga ccatttcaca
    29521 ctcggtcatc agcagttcgc cgacgttcac cgcaaatccc agctcttccg gcataatcga
    29581 cacgtcgatg taaccactgc ggatttcgcc cgcgaacggg tgattgcgtc cgtaaccgcg
    29641 ctgggtagag taggccagcg ccagcaaata gccttcgtcg ccgcgcatca actgctgcaa
    29701 acgggaagaa cgtgaacagg ggtaaactgg cggagtgcgg gtgatgtcat ccggctgtgc
    29761 gccgctatcc tcttcaaact tcgccagccc ctgacgcgcc agcaggctga aaacgtgcgg
    29821 cgacggggat tgttcgctgt ctgcggtggt cagcgtcggc gcttcgccgt ttgccagcag
    29881 ggtgaaatcg agcaggcgat gggtgtagtc gtaggttggg ccaagcaact gaccgccggg
    29941 aatatcttta taaaccgctg aaatacgccg ttcgagacgc atctccgtgg tgtcgagcgg
    30001 ctcgcttacc gccagcttcg ccagcgtggt gcggtaggcg cgtagcagga aaatcgcttc
    30061 gacgttatcg ccgctggcct gtttcagcgc cagcgccgcc agttcgcggt cggcaatgcc
    30121 gccttcggtc atcacgcgat ctactgcaag attaagctgc tgttcaatct gggcgacgct
    30181 cagttcaggc aaatcggtat tgccccggcg tcggctctct tgcagggcgt gggcggcgtc
    30241 gatctccttc tcgccccctt tcacggcaac gtacatcagc acacctccac atgagtggtt
    30301 cgcgggatag ccagcagccg ctcgccgcag gtcaggatca ggtcgatgcc gagcgggaac
    30361 gggtgcgggc gctcggtgag ttcgtgcaga atgcactccg gcagctgcgg agcgatcatt
    30421 cgttcttcgg caatacccgc gccagtaagg cgcagcatgc gcccgccgct caggctgacg
    30481 acctgtaaaa tcagcgtcgc gcccgcttcc ggcgcaacag cggtgccgct ggaaagagcg
    30541 ttgagctgtt cgctggaaat cgcctcatcc gttaccgcga aggtcgccag ttccgactga
    30601 ttgaccagcg gcgcgttggt atgaaaacgc aggctctggc tgacgatatc gttacttaat
    30661 ggcgcggaaa gccacaccgg cgtgtcgtta tcagccagcg tcagcagcac gctggtggtg
    30721 gcgatattca gcggctgcca gccgcgtttg agctgatgca gggcgacaat cacgcccggc
    30781 tcgctcatgg cctttaacag gcgacgaaaa ctgtgctggg catcctgcac aggaagcata
    30841 aaagcggttt ccagggtcat gcgttgtctc cgcgaaccat cgtaaagaag tcgacccggc
    30901 tggcgttcac ttcggcctgg cgtgcggcaa tgcgtgccat acggtcagcg tccagtgggg
    30961 caataagggt ttctgataag ttttgaaaat ggcgagattg ctgcatcaac gcgtcaatca
    31021 gcgcgcagcg ttcggcgtgc tgtttgtcgc gccccagcac ccagctgtag ccgagggggc
    31081 cgtcagtcag gcgtacggcg gcgcgggtca gcgtggcgtc gccggcaaaa aagcgttcgc
    31141 cggtaccgcc cattcgcggc tgaatctgta ccaggccggt ttcggcggtg cggatcactt
    31201 catagttggc ggtgatgttc agcgcgttca ggcgggctgc cagttcagcc ggttggctgt
    31261 gcgccagcat ggacatccag tgctggcggg tcgcgatatc tgcgtgcatt cagtgctcca
    31321 tagtgaattc aatcatgtcg gcgcgcgtca ggctgacgga gtactccgcc gggctgcttt
    31381 caccgtcacg gtggttaagg gtgcgcacgc acagcagcgg cgacatattc gggatttcaa
    31441 gacgctggca ctctttggcc tgggcgcggc gggcgctgat ccgcgtctgg ctgcggcgca
    31501 gcgcaatccc ggtttgctcg cgcagaaaat cgtgcagcga gccgctgtcg aagcgttgca
    31561 gcgtcggcca aagggtgagg tccgcaaagt agtggtcgat taaacagagc gcgatgccgt
    31621 tgacccggcg cagggtgcgc aggtggatga cgttctcccc ctcggttatc cccagcgcgt
    31681 cagcgacgtg accagatgcc gggcgcagca ccgacagcag tttttcgctg gtgggatggc
    31741 tgccctgatc cagcaggttc tggctaaaac gcgcctgggc gttgagcggg taatcgaacg
    31801 ggcgcatcag caccagcacg ccgacgccct gacggcgctg tacccagcct ttttccacca
    31861 gttggtcgat ggcgcggcgc agggtgtggc gatttacctc aaagcgcgcc gccagctgct
    31921 gctcggcggg aagatagtcg ccgcagcgat agtgttgacg aagctcctgc tcaagttttg
    31981 cggctatctc ctgatagcgt gttgggtagc tggtcggatg tgtagacaag tgcatagata
    32041 tcaatgcctc gcttatcaga taaagtgctt acgcaaccgt tgagagagga aatccagcag
    32101 gctgaccgtg acgatgataa gcaccatcag ggcgcaggtt tgttggaact gaaaaccgcg
    32161 aatcgcttcc cacagggtga cgccgatccc gcctgcaccg accatgccga cgacggtcgc
    32221 cgagcggacg ttggattcga agcgatagag ggagtaggag atcagcagcg gcattacctg
    32281 tggcagcacg ccgtaaagga tctcttccag cttgttggcg ccggtggcgc gaatgccttc
    32341 taccgggccg ggttcgatcg cttccaccgc ttcggaaagc agcttggaga gcacgccggt
    32401 ggtgtggata aatagcgcca gcacgccagc gaacgggccg agaccgacgg cgaccacgaa
    32461 cagcatggcg aagaccattt cgttaatggc gcggcaggca tccatcaggc ggcgaacggg
    32521 ctggtaaacc caccacggca ccaggttttc ggcgctcatc agaccgaagg ggatggagag
    32581 aaccactgcc agcgcggtgc cccagacggc gatttgcagc gtgacggcca tttcggtgag
    32641 gtaatcctgc cactggctga aatcgggcgg gaagaagtcg gcaacgaagg ttgccatgtt
    32701 gccgccgtct ttaatcagcg taagcggggc catttccgcg ccctgccacg agacgaccag
    32761 cacggcgaga acaacggccc agctcagaag cgagaaccag ctacgcttgg gtggggcgat
    32821 ggtgatggtt tgcatgtttg gctccgttta atgttttctc cctctccctg tgggagaggg
    32881 ccgggatgag ggcatcagcg tgcgcatttc cccctcaccc taaccctctc cctcaaggga
    32941 gaggggaccg atcgagcaca ttactgcacc gctttactca ccgaactcat cgcgcttaac
    33001 gcgttgttca ggcggtccaa gtcatccagc tgcgcctgaa tctcggtggt tttcgccagc
    33061 ttgtcctgct cattcagtcc tttattgctt tttacgccct gcatctcttt aaacagtgcg
    33121 agctggcgaa tcggcaccag ttgcaggtcg ctggaggcgc ggaacggggc ccagcccagg
    33181 cgttccagca ccgctttctc ttccggcgtt tttccgtagt tcataaagaa gtcgtagatc
    33241 ttgtctttgg tggtttcgga gagattcttg cgccagacga tcggatcgcc tgggatcagc
    33301 ggcgacttcc agatcacctt cagttctttc agcttctctg gcgcggaggt tttcagcttg
    33361 tcgaggtttt cggtgttgtt ggtggcaaca tccacctgct tgttggcgac ggccagcgcg
    33421 ttggtttcat gcccagcgtt gacggtgcgc ttaaagtcgc tggcggagat attgtttttg
    33481 gcaaagacgt agtaaccggg gacgaggaag ccagaggtgg agttaggatc gtcattgccg
    33541 aaggtgagat ctttccgctt cgccagcaga tcgttcaggt tgttgatcgg actatctttg
    33601 ttgacgatca acacgctcca gtaacctggc gatccatccg ccgcgaccgt ctgggcgaag
    33661 acctggccgt tggcgcgatc caccgcttcc attgccgaca ggttgccgta ccaggcgata
    33721 tccactttat tgaagcgcat tccctggata atgcctgcgt agtctggggc aaagaaggcg
    33781 ttcaccttca cgcccagctt cttctccata tcctgtaaga atggcgtcca ttgcggtttc
    33841 aggttttgct gtgattccgt tgaaataatg ccgaaattca gcgccttttc ctgctcttcg
    33901 gcgtgtgccg ggcttaacag ggtgctgagg ctgaacatgc tggtgaaggc cagcgaggca
    33961 attatcttag cgttcattcg ttttcctcaa tgatggggat gtcaggcagc tttcgcgttc
    34021 tcttcgatgc gattaatgct gcggtagaga tggtcaaaac gttcgttatc aaactgttgg
    34081 ctgctgccgt cgtagaaaac gtgcccctgg cgcagggcga cgatgcgttc gcagtagcgc
    34141 agggcgtaat ccacctgatg cagcgtgacg actacggtga tgccgtcgtt ctgattgatg
    34201 tcgcgcaggg tgtccatcac gatgcgggcg gattccgggt ccagcgaggc gatgggttca
    34261 tcggccagaa tcaccttcgc ctgctgcatc agcgcgcggg caatcgccac acgctgctgc
    34321 tgtccgccgg agagggtgga aacgcgttga tgggcaaaat gcaccatgcc aacgcgggtc
    34381 agcgcctgta acgcgcgttg tttctgctcg cgggtgaacc agctaaaaca ggtgcgccag
    34441 aacggcgtgc tgccgagcgc gccaatcagc acgttctcca gtacgctcag gcgattcacc
    34501 aggttgaatt gttggaagat gtagccggta tgggcgcggc ttttgcggat atcgcgcgcc
    34561 agacggcctt cgcgctggac tgtgcggccc agcagctcga tatggctgcc ggcggattta
    34621 tcgccggtaa tcaaaccgct taagtgacgt aaaagtgtgg atttgccgga acccgacggc
    34681 ccaagcagag ccaccatttc actgtgatga atgttcagat caaccgcatg cagcgcttga
    34741 tgctgattga aggttttggc gagcttctcg acacggataa tcgtttgcat gatgcagcct
    34801 ccctaaaaaa gtggctccat cgtggcggat tattgtgacg atttggttaa ttaaaagtga
    34861 ctaacagatg aagagttaac gggaattcga tgacagtgcg ggagggcggg cctcccggcg
    34921 ggttattgcg ttggttgttg tttgacgaca ttaatcatcc acggtacgcc aaatttatcg
    34981 gtgactttgc cgaagccatg cgcccagaaa gtttcctgcc aggccatttc aatttttccg
    35041 ttagcggcaa ggttatcaaa ccagcgtttt ccttcttcga cctgttgcga atcgagcacc
    35101 agcgtaaagc cggagtagct ggcttttcct gacggtatag catcgctcat catgatgtcg
    35161 cttccggcaa tgcgcacgtt ggcgtgggcg atggcggtat cgggaaattg cattccggaa
    35221 gggcagttct cggcgctgtc ctgcgctgat tttggcattt cgccgaagct gattttatag
    35281 agcagttccg cgcccaacgt acgttgataa taggcaatcg cgtcggaaca gttaccggca
    35341 aaagagaggt agggacttaa cggcatgatg gtgacctcag ttaagagaag ccagttaagt
    35401 gtagttcgaa atttatacag atgagaggga agcctgataa ggcgcggtca gcgtcgcatc
    35461 aggcagtcgg catcgttgcc ggatgtggcg cgagcgcctt atccggctta ccgatcacaa
    35521 tacaatcagt tctttttcac aaactcagat ttcagtttca tcggaccaaa accgtcgatt
    35581 ttgcaatcga tgttatggtc gccttcaacc aggcggatgt ttttcacttt ggtgccaatt
    35641 ttcagcatcg aagagctacc tttcaccttc agatctttaa tgatggtaac gctgtcgccg
    35701 tcagccagca gattgccgtt agcatcttta acgatcagct cgtcgctttc ctgtgcaggt
    35761 tctgtgtcgt tccattcgta ggcacattcc gggcagatgt acatgccgtt atcttcgtaa
    35821 gtgtattcgg agttgcattt tgggcagtgt ggtaatgaca tgtggatttc ctcaaaagtc
    35881 agcagggcaa aagcgccaaa aaacggcaga ttgccgaaaa aggccgcaat tatacacaaa
    35941 atccctgagt ttgtcggggg tactgctgat tttagacgac gatcgttttc atttgaaagg
    36001 aaacgcaaaa agaaaataat ctttttgcct ctatttatta tttaagataa tgttaatgct
    36061 ctacctgttc tttttttatt cac
//
LOCUS       DAJVTY010000021        35013 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVTY010000021 DAJVTY010000000
VERSION     DAJVTY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35013
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.021"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OQQ66_000958"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OQQ66_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OQQ66_000959"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OQQ66_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1903969.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OQQ66_000960"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OQQ66_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1903970.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OQQ66_000961"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OQQ66_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1903971.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OQQ66_000962"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OQQ66_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1903972.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OQQ66_000963"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OQQ66_000963"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1903973.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OQQ66_000964"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OQQ66_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1903974.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIVYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OQQ66_000965"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OQQ66_000965"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1903975.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OQQ66_000966"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OQQ66_000966"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1903976.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OQQ66_000967"
     CDS             complement(14507..15139)
                     /locus_tag="OQQ66_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1903977.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OQQ66_000968"
     CDS             15435..15710
                     /locus_tag="OQQ66_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1903978.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OQQ66_000969"
     CDS             complement(15787..16029)
                     /locus_tag="OQQ66_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1903979.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OQQ66_000970"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OQQ66_000970"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1903980.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OQQ66_000971"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OQQ66_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1903981.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OQQ66_000972"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OQQ66_000972"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1903982.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21106
                     /gene="ypdA"
                     /locus_tag="OQQ66_000973"
     CDS             19409..21106
                     /gene="ypdA"
                     /locus_tag="OQQ66_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS1903983.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21121..21855
                     /gene="ypdB"
                     /locus_tag="OQQ66_000974"
     CDS             21121..21855
                     /gene="ypdB"
                     /locus_tag="OQQ66_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1903984.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21868..22725
                     /locus_tag="OQQ66_000975"
     CDS             21868..22725
                     /locus_tag="OQQ66_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1903985.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22728..25223)
                     /gene="ptsP"
                     /locus_tag="OQQ66_000976"
     CDS             complement(22728..25223)
                     /gene="ptsP"
                     /locus_tag="OQQ66_000976"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1903986.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25248..26285)
                     /gene="ypdE"
                     /locus_tag="OQQ66_000977"
     CDS             complement(25248..26285)
                     /gene="ypdE"
                     /locus_tag="OQQ66_000977"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1903987.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26285..27370)
                     /gene="ypdF"
                     /locus_tag="OQQ66_000978"
     CDS             complement(26285..27370)
                     /gene="ypdF"
                     /locus_tag="OQQ66_000978"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1903988.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27385..28632)
                     /locus_tag="OQQ66_000979"
     CDS             complement(27385..28632)
                     /locus_tag="OQQ66_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1903989.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28654..28980)
                     /locus_tag="OQQ66_000980"
     CDS             complement(28654..28980)
                     /locus_tag="OQQ66_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1903990.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29199..30164)
                     /gene="glk"
                     /locus_tag="OQQ66_000981"
     CDS             complement(29199..30164)
                     /gene="glk"
                     /locus_tag="OQQ66_000981"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1903991.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30368..31624
                     /locus_tag="OQQ66_000982"
     CDS             30368..31624
                     /locus_tag="OQQ66_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1903992.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31739..32065
                     /locus_tag="OQQ66_000983"
     CDS             31739..32065
                     /locus_tag="OQQ66_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1903993.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32206..33444)
                     /locus_tag="OQQ66_000984"
     CDS             complement(32206..33444)
                     /locus_tag="OQQ66_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1903994.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33780..34982
                     /gene="nupC"
                     /locus_tag="OQQ66_000985"
     CDS             33780..34982
                     /gene="nupC"
                     /locus_tag="OQQ66_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1903995.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9234 a         8692 c         8083 g         9004 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaagggggaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataaacaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgaat tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tacacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttcaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccgggc acggcatcga tccaaaggtg attgagcggg tcgaagcgaa
    20941 tgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta
    21001 tggcgagggg ctgcatatcc gccgcctgga gccggggacg gaaattgcgt tttacattcc
    21061 taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt
    21121 gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt
    21181 aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag
    21241 tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat
    21301 ggcgtgttgc tggcgcaaaa catcagccag ttcgcccata aaccgtttat tgtgttcatc
    21361 accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga ctacattctc
    21421 aaaccgtatc aggagtcacg tattaccggg atgctgcaaa aactggaagc ggcctggcaa
    21481 caacagcaga ccagcagtac gcctgccgcg acggtaacgc gtgagaatga caccattaat
    21541 ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta cgccgaagcg
    21601 cacgagaaaa tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt
    21661 accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg ctcattttgt
    21721 gtcaatctga acaaaatacg cgaaatcgaa ccgtggttta ataacaccta cattctgcga
    21781 ctgaaagatc tggattttga agtgccggtc agccgcagca aagtgaaaga atttcgccag
    21841 ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa
    21901 tttttcgccg atctgttcag cggcctggtg cttaactcgc aactactggg gcgcgtctgg
    21961 tttgccagcc agcctgcctc gttaccggtg ggcagtttat gtattgattt tccccgtctg
    22021 gatatcgtgc tgcgcggcga atacggcaac ctgctggaag caaagcagca gcgaatggtg
    22081 gaaggagaaa tgctgtttat tccggcacgc gcggctaatt taccgatcaa caacaaaccg
    22141 gtgatgctgt taagcctggt gttcgcaccg acctggcttg ggttatcgtt ttacgatagc
    22201 cgcaccacgt cgttgttgca tcctgctcgc cagatccagc ttcccagcct gcaacgcggt
    22261 gaaggtgaag cgatgcttac cgccctcact catcttagcc gttcgccgct ggagcagaat
    22321 atcattcagc cactggtgtt aagtttgctg catctttgcc gcaacgtggt gaatatgccg
    22381 ccgggtaatt cgcaaccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag
    22441 gataattata cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc
    22501 aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt
    22561 tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc aattcatgaa
    22621 gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag
    22681 tttggtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat
    22741 tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg
    22801 aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct
    22861 tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag
    22921 tcaatcggtt tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg
    22981 gtgtgcggga tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc ccgctgccag
    23041 acatcttctt ccagctcaaa cggatgttca gtgcgcccgt taacgccgag gttgccgcac
    23101 aggaactgga tcgcctgctc tttattgcta aaatcctgat caacaaagat attctccagc
    23161 gccagcagtg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc
    23221 tgcgcactgc ggcattcaca tgcctgacgc gccagttccc gacacgcctc gctatccagt
    23281 tgacgaagct ggcttttcac cgccggaata cgcgggctac tcatactcag ctcatccagg
    23341 cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct
    23401 acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg caggaacgat
    23461 ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac cgcatacaga
    23521 tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtggtcg
    23581 atgatgtagc acaccgacgg cacttccacc atgatcccaa gcgtaatcgt ctctgcatga
    23641 cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccctt cacccataag
    23701 atctgatcga gactgtggac catcgggatc atcaactggg cgttgccaaa actggcggcg
    23761 cgcaaaatcg cccgtagttg agtgcggaac aggccagcaa attccgggta aatacgtacc
    23821 gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta
    23881 tcgccaccga tgtccattgt gcggaagata atcggcttgt cgcccgctgc cagtagcacc
    23941 tgctggtagg cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc catatagagc
    24001 atttcagtac ggaacagtcc aacgccttcc gcgccgttgg caaacgcgcc tggcgcttcc
    24061 agagcggtgc cgatattcgc cgcaatatca atacgcttgt tatcacggga ataggccagc
    24121 tgcgcggcgg cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg tgcgacctga
    24181 taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg ggcgtcaagc
    24241 accgccggtt gcccggcata acgggcaatc gcatccagcg gcaggccgct cagtaccggg
    24301 atcgccgagg cgcgggccag aatcagggta tgcgaggtgc gcccagtttt ctccagaatc
    24361 atgcctgcaa gattttttaa atcgaggctc aaaaactggc ttggggttaa atcctcggct
    24421 accagaatag tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc cggccaggtg
    24481 atatgcagca actgttcgct aatatcgcga atgtcgctaa cacgttcgcg cagataatca
    24541 ctggcagaag cagaaagttt ggcgcaaacc tgctccatat tgctgatgat cgccgccccc
    24601 agcccttgat gctgttctgt catcagacga cggatattgc ccgcgaattc atcatcctga
    24661 atcagcgaca aatgggcgct aaggatagtt ttgctttcgc cgtcactctc acgcaattgc
    24721 tggttcagtt gctcggcaag cgttgcaagg ctgtgctcca gccgggtgga atcttgcgca
    24781 ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc
    24841 acgccgacgc tgcttgccag cacattgccg tacagtaaat ccgggttcag gcggcttaat
    24901 gaacgcggca gcggatgcgc cgtcagttct gcctgcgttg gctggatgct gtcgctgtcg
    24961 ataaagcgca cctggatgta ctcttccaac acgcgctgcg cctgctcttc atcgctgccg
    25021 ctaatgttca ggctgcaact gtcattaaat aaagtaccgg tgccaatcag cgccagcgag
    25081 cttttcgcat cggcctttgc gttctggcga tggttgataa aggtgatttc gctttgccac
    25141 tggctgcact gttctttaag ttcccacgcc ggacgagcat gaagaccgtt tggcaaagga
    25201 cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc
    25261 gtcagttgaa caaccgtctc acgcgtaaga cgtttaataa gggcagataa aagctgctgc
    25321 atctgcaaaa tgtcacggca atcggcaatc gatgcggcgc aatgtccgtg gcgggttgct
    25381 ggccccatca ccactgtagg cacgccggtg ccggttaagt gcaccgctcc gccgtccgtg
    25441 ccgccgttgc tgaacatatc tgcctgtaac ggcacgccaa tttctgccgc cacggtttcg
    25501 atccacgcgg taagttttgg cggcgcaatc agcgacttgt cgcttaacac cagcatcggc
    25561 ccgttacaaa tctgacgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg
    25621 gtatcaagca caatggcgac atccggcgac accgcgcggg tggcagtttg cccgccgcgt
    25681 acccccacct cttcgctgga actggccacc agccacactt ccgcaggcag ttcagcgctg
    25741 tgtagttcgc gcagtaacgt caccagcagg tagcaaccga ggcggtcatc aaaggctttc
    25801 cccatcaccc gctggtgagg gagaacctga aaagttgtat caaaggtgac gcgatcgcct
    25861 ggacgaattc ccgcctgcat cacttcgtca tacgagcgcg taccaatgtc cacgcgcatg
    25921 gcgctgacgt cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca
    25981 cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc
    26041 agcacatcaa ttgccccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata
    26101 tgggcgcaga tcatcacctt cggccctgtc gattcattga ggcggatcag caccgatccc
    26161 agaccatcaa atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc
    26221 acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgcttttaa tagcgataaa
    26281 tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc
    26341 cttgcggggt aaccagcaca acatcttcga tgcgcacgcc cccttgccct ggcaaataaa
    26401 tccccggctc cacggtcagc aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa
    26461 aacgcggatc ttcatggact tcaatgccga tagcgtgacc agtgttatga ccgaaatagt
    26521 cgccaaatcc agcctcggta atgacccggc gcgcggcgtc gtcaatctgc tggcagcgca
    26581 cgccggggcg aattgcagag attgccgcga gctgtgcctg caggacaatc tgatagacgt
    26641 caaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca
    26701 tatcagagca gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg
    26761 caacaatctt gtcgctggct ttgccgtgcg gcagcacccc gcgccagccg ctggcgacaa
    26821 tggtgtcaaa agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg
    26881 ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat
    26941 cggcaatccc acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca
    27001 gcacatccgg cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg
    27061 tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga
    27121 tttgattgac gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt
    27181 gcgcacgggc ttccacctca acgaaatagc tcgaatctac cagaatgtgc gcactttcac
    27241 ggctaataac cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc
    27301 gtgaggagag aagcactgca tccagttgtt gctccttaag ccagtcgcgc agcgaagcga
    27361 gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa
    27421 catcatcaga cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc
    27481 cgccatatag acgccaaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa
    27541 ccattgcact gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata
    27601 cgacggaatg gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc
    27661 cataaaggca aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcg
    27721 tttgccggtt aaacgacggt caataatggt cgccagcccc agaccaatcg gaggaataac
    27781 gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac
    27841 gaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc
    27901 cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacggtac ggataacgcc
    27961 gttgatccag ccgccaaagg gggtaatgac gtagtacatc gccagcatga caaaaatggc
    28021 ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat
    28081 tttctggttc atccacttca caaggtaacc cgcgacgata gaaataatca gcgcaccaat
    28141 gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag
    28201 cagttgcgtc ggctgggtag acatcaagcc gccgataaaa ccagccggga acgccagttt
    28261 gccaccgata gagttcgcca caaaagcagc aaacatcgga atggcaaaac caaacaacac
    28321 gccgccgaag gactgcgaca gccaggcaaa tttcagtaag gagaggtcga aaccggagaa
    28381 tttcccgcta ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata
    28441 agcaatcagc tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg
    28501 tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg
    28561 cggattctta actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt
    28621 tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc
    28681 ttcgatttct ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata
    28741 aacgtcgcgt gattcgaaac gctcattatc ttccggggta actgccacgg agtggatgat
    28801 gatggttgct tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg
    28861 ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccaacg cctgagcggc
    28921 cataaaggtg tgagccaggc ccattgggca ggcacataag gcaatcagtt tcttactcat
    28981 cgttaattcc tcattcagtg atagttgaca agtgaaatta tgaacgtgca aaacaaatcg
    29041 ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac
    29101 gcaggtcggc cttgtgaggg gacacaaaga aaaattgaat taactgtatg atttaaacga
    29161 ttatcgggag agttacctcc cgatataaca ggaaggattt acagaatgtg ccctaaggtc
    29221 tggcgtaaat gtgcaccgga accgagaaga cccggattgt catggacgat gagatacacc
    29281 ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcagc acggaaacca
    29341 gaggctttga agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca
    29401 aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc
    29461 gacaacgcgc ggcggcaatc ggtacagctg tcagccagcg cgcgttcggt aatatctttt
    29521 ggcttgagat tttctggcag gcggttgtca gctttcacaa ttgcgcgata caaattcacc
    29581 agcccagggc cagaaagcac gcgctccgcc gaaacatgac caatttccgc acgcaatatt
    29641 tcgaggataa tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc
    29701 ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag ccctgttccg
    29761 gcaccgtaaa ccgcaatagg tttaccttct accggttctg cgccaccaaa ctgaatcaga
    29821 tgctcttttt tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc
    29881 agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg
    29941 gtcattgcca cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc
    30001 tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc
    30061 cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt
    30121 gcgttggtgc cgcccacatc accgactaat gcatacttta tcattcttca actgctccgc
    30181 taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg
    30241 aaaggcaggc tccctgtaaa tatcgatctg ggtcacacat ttactttatc gtttcagcac
    30301 caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga
    30361 atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc
    30421 aattgggatt gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca
    30481 gaatttgctc tggcaacgac tgccgggaac tctggggata gcccaggatt cacctctctg
    30541 gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca
    30601 gggtcatgcc ggaccagacc ccgcctgtga accgctgatc ggcgcaccag ttccgccctc
    30661 tgcgctacca ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg
    30721 gccggaacat ccgatcatga ccatcaatat cgcccttgcc gtcgccattg gcgctcgtct
    30781 gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc
    30841 gctgtttggc acacctgttg cagcggcgtt gatattttcg caaaccttaa atggcagtag
    30901 tgaagttccg ctatgggatc gtctctttgc gccgttaatg gcggcagcag ctggtgcact
    30961 aactaccgga ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat
    31021 ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcagggat
    31081 ggtcgccgta tggtgcttac cacggttgca cgcgatgatg aatcaaatga aaaatccggt
    31141 gctcgtgctg ggtattggcg gatttattct cggtattctg ggggttattg gtggaccagt
    31201 ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac
    31261 cagcgattac tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag
    31321 tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat
    31381 gctgcatgag cacgtacccg ccgtaccagc ggcaataacc gtttcttgcg ctattctcgg
    31441 catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg cggtcgttgt
    31501 acccaatacc acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt
    31561 agcaggtaag ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataacgt
    31621 ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg gggctttacc
    31681 tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat
    31741 gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaacgc
    31801 aggtgaaatc accctactgc catcaatcaa attacaaatt ggcgatcgcg atcattacgg
    31861 taattactgg gacggtggtc actggcgcga ccgtgactac tggcatcgca attatgagtg
    31921 gcgcaaaaac cgttggtggc gtcatgataa tggctatcat cgtggctggg ataagcgtaa
    31981 agcgtatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg
    32041 ccgcggacat ggacaccgcc attaaacaca ttcgccaatg gagcacaatg cctgatgcga
    32101 cgctagcgcg tcttatcagg cctacaaacc ggatctttaa cgcgtagggc ggataaggcg
    32161 tttacgccgc atccgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc
    32221 cgtccccacc aacaaccaga tattcagcgc cacgaccagc actacaatca cccagcctgt
    32281 ctgttttacg cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat
    32341 cagcagtgga accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag
    32401 aatccgtgtc ggatctaatc ccatcagaat gacaataaat gacggcagca tggtgactgt
    32461 acgacgcacc cacagcggga tatggaagcg aatgaacccc tgcatcacca cctgccctgc
    32521 cagtgtcccc actaccgttg aggacagtcc ggcagcaacc agacttaacc caaagaccgt
    32581 tgccgcagca tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcggcaac
    32641 accagtatga ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac
    32701 aaaaccggca atagtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg
    32761 acgcgaaccg ccatgtaaat gctgagtgag cgaggagtgc aaataaatca catgcggcat
    32821 aatcgtcgcc cctaacacgc ctgctgccag gaagaccgct tccgaagtag gtaaactcgg
    32881 gatcaccatt cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat
    32941 gtaagccgcg gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc
    33001 gcgacgttgc agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa
    33061 caacgaaaca ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc
    33121 ggttgccatc gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata
    33181 gtgatcgcga atctgctccg ccagattttt accggtggca atccctagtt tggcagagag
    33241 gatctgaatc agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc
    33301 gaagctggca cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc
    33361 cgcaatgaac gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct
    33421 actctcaacg cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca
    33481 tgctatgata aacgagaatg attatcaaat tcatttaaat ggattgtggt gatttctgtg
    33541 atagaccagg attatgacta cgaaaagatt gatgacttga atgttatggt tatgtttaat
    33601 gttagcacat ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacaga
    33661 tcactatttt tgaagcctgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc
    33721 ttaaccatgt tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata
    33781 tggaccgcgt ccttcatttt gtactggcac ttgccgttgt tgcgattctc gcactgctgg
    33841 taagcagcga ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag
    33901 tgttactggc gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct
    33961 ccgaaatgtt cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta
    34021 gcatgaatga tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta
    34081 tctctgcact gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatc atccgcgcaa
    34141 ttggtttcct gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca
    34201 gctccctgat tctgggtcag tctgaaaact ttattgccta taaagatatc ctcggcaaaa
    34261 tctcccgtaa tcgtatgtac accatggctg ccacggcaat gtccaccgtg tcgatgtcca
    34321 tcgttggtgc atacatgacc atgctggaac cgaaatacgt cgttgctgcg ctggtattga
    34381 acatgttcag cacctttatc gtgctgtcgc tgatcaaccc ttaccgtgtt gatgccagtg
    34441 aagaaaacat tcagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg
    34501 aatacattct ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg atcggcttta
    34561 tcgccctgat cgctgcgctg aacgcactgt ttgccaccgt gactggctgg tttggctaca
    34621 gcatctcctt ccagggcatt ctgggctaca tcttctatcc gattgcatgg gtgatgggtg
    34681 ttccttccag tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg
    34741 agttcgttgc gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag
    34801 gcatcatctc tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcag
    34861 gtgcagttaa aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac
    34921 tggtttacgg ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt
    34981 aagaccatac ataaaaaagc cggggataat tcc
//
LOCUS       DAJVTY010000022        34690 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVTY010000022 DAJVTY010000000
VERSION     DAJVTY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34690
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.022"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..3724)
                     /locus_tag="OQQ66_000986"
     CDS             complement(<1..3724)
                     /locus_tag="OQQ66_000986"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCS1903996.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTVRPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGR
                     TPGHRTPLWQQRLRASQLLEIAQGYPDFPVILEILRECLQDVYDLPALERLMRRLNGG
                     EIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVD
                     PGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEV
                     ASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLR
                     DLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRR
                     LRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMR
                     VIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVAL
                     HLQAYAAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSA
                     LQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTR"
     gene            complement(3817..4464)
                     /gene="rnt"
                     /locus_tag="OQQ66_000987"
     CDS             complement(3817..4464)
                     /gene="rnt"
                     /locus_tag="OQQ66_000987"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1903997.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(4567..4974)
                     /gene="gloA"
                     /locus_tag="OQQ66_000988"
     CDS             complement(4567..4974)
                     /gene="gloA"
                     /locus_tag="OQQ66_000988"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1903998.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5055..6152)
                     /gene="nemA"
                     /locus_tag="OQQ66_000989"
                     /pseudo
     CDS             complement(5055..6152)
                     /gene="nemA"
                     /locus_tag="OQQ66_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(6189..6788)
                     /gene="nemR"
                     /locus_tag="OQQ66_000990"
     CDS             complement(6189..6788)
                     /gene="nemR"
                     /locus_tag="OQQ66_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1903999.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6891..7130
                     /locus_tag="OQQ66_000991"
     CDS             6891..7130
                     /locus_tag="OQQ66_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1904000.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7179..8075
                     /locus_tag="OQQ66_000992"
     CDS             7179..8075
                     /locus_tag="OQQ66_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1904001.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            8156..8677
                     /gene="sodC"
                     /locus_tag="OQQ66_000993"
     CDS             8156..8677
                     /gene="sodC"
                     /locus_tag="OQQ66_000993"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1904002.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(8678..10689)
                     /locus_tag="OQQ66_000994"
                     /pseudo
     CDS             complement(8678..10689)
                     /locus_tag="OQQ66_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(10689..11546)
                     /locus_tag="OQQ66_000995"
     CDS             complement(10689..11546)
                     /locus_tag="OQQ66_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1904003.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(11612..11785)
                     /locus_tag="OQQ66_000996"
     CDS             complement(11612..11785)
                     /locus_tag="OQQ66_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1904004.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            11986..12420
                     /gene="slyA"
                     /locus_tag="OQQ66_000997"
     CDS             11986..12420
                     /gene="slyA"
                     /locus_tag="OQQ66_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1904005.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(12467..12934)
                     /gene="slyB"
                     /locus_tag="OQQ66_000998"
     CDS             complement(12467..12934)
                     /gene="slyB"
                     /locus_tag="OQQ66_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1904006.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            13208..14317
                     /gene="anmK"
                     /locus_tag="OQQ66_000999"
     CDS             13208..14317
                     /gene="anmK"
                     /locus_tag="OQQ66_000999"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1904007.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            14415..14744
                     /gene="mliC"
                     /locus_tag="OQQ66_001000"
     CDS             14415..14744
                     /gene="mliC"
                     /locus_tag="OQQ66_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1904008.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            14803..15459
                     /gene="pdxH"
                     /locus_tag="OQQ66_001001"
     CDS             14803..15459
                     /gene="pdxH"
                     /locus_tag="OQQ66_001001"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1904009.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            15588..16862
                     /gene="tyrS"
                     /locus_tag="OQQ66_001002"
     CDS             15588..16862
                     /gene="tyrS"
                     /locus_tag="OQQ66_001002"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1904010.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            16921..17784
                     /gene="pdxY"
                     /locus_tag="OQQ66_001003"
     CDS             16921..17784
                     /gene="pdxY"
                     /locus_tag="OQQ66_001003"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1904011.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(17828..18433)
                     /gene="gstA"
                     /locus_tag="OQQ66_001004"
     CDS             complement(17828..18433)
                     /gene="gstA"
                     /locus_tag="OQQ66_001004"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1904012.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(18539..20041)
                     /gene="dtpA"
                     /locus_tag="OQQ66_001005"
     CDS             complement(18539..20041)
                     /gene="dtpA"
                     /locus_tag="OQQ66_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1904013.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(20653..21288)
                     /gene="nth"
                     /locus_tag="OQQ66_001006"
     CDS             complement(20653..21288)
                     /gene="nth"
                     /locus_tag="OQQ66_001006"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1904014.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(21288..21983)
                     /gene="rsxE"
                     /locus_tag="OQQ66_001007"
     CDS             complement(21288..21983)
                     /gene="rsxE"
                     /locus_tag="OQQ66_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1904015.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(21987..22607)
                     /gene="rsxG"
                     /locus_tag="OQQ66_001008"
     CDS             complement(21987..22607)
                     /gene="rsxG"
                     /locus_tag="OQQ66_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1904016.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(22611..23669)
                     /gene="rsxD"
                     /locus_tag="OQQ66_001009"
     CDS             complement(22611..23669)
                     /gene="rsxD"
                     /locus_tag="OQQ66_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1904017.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(23670..25892)
                     /gene="rsxC"
                     /locus_tag="OQQ66_001010"
     CDS             complement(23670..25892)
                     /gene="rsxC"
                     /locus_tag="OQQ66_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1904018.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(25885..26463)
                     /gene="rsxB"
                     /locus_tag="OQQ66_001011"
     CDS             complement(25885..26463)
                     /gene="rsxB"
                     /locus_tag="OQQ66_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1904019.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(26463..27044)
                     /gene="rsxA"
                     /locus_tag="OQQ66_001012"
     CDS             complement(26463..27044)
                     /gene="rsxA"
                     /locus_tag="OQQ66_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1904020.1"
                     /translation="MTDYLLLFVGTILVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(27121..27561)
                     /locus_tag="OQQ66_001013"
     CDS             complement(27121..27561)
                     /locus_tag="OQQ66_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1904021.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(27647..27862)
                     /gene="ydgT"
                     /locus_tag="OQQ66_001014"
     CDS             complement(27647..27862)
                     /gene="ydgT"
                     /locus_tag="OQQ66_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1904022.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(28135..28260)
                     /gene="blr"
                     /locus_tag="OQQ66_001015"
     CDS             complement(28135..28260)
                     /gene="blr"
                     /locus_tag="OQQ66_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1904023.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            28473..29513
                     /locus_tag="OQQ66_001016"
     CDS             28473..29513
                     /locus_tag="OQQ66_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1904024.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(29548..30549)
                     /gene="add"
                     /locus_tag="OQQ66_001017"
     CDS             complement(29548..30549)
                     /gene="add"
                     /locus_tag="OQQ66_001017"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1904025.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(30652..31824)
                     /gene="malY"
                     /locus_tag="OQQ66_001018"
     CDS             complement(30652..31824)
                     /gene="malY"
                     /locus_tag="OQQ66_001018"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1904026.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCNMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(31834..33426)
                     /gene="malX"
                     /locus_tag="OQQ66_001019"
     CDS             complement(31834..33426)
                     /gene="malX"
                     /locus_tag="OQQ66_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1904027.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            33601..34629
                     /gene="malI"
                     /locus_tag="OQQ66_001020"
     CDS             33601..34629
                     /gene="malI"
                     /locus_tag="OQQ66_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1904028.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         8704 a         8897 c         9023 g         8066 t
ORIGIN      
        1 ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
       61 tgtcgctggt gatgacgcct tgccagacca gcgcccataa cgcctcttgc agggcagaga
      121 gatcaaccga ttcgccgatt ttgtcgcgta tccgttggct gatttgttgt gcaaaccagg
      181 ctcctccgtc agccagaaca gcgactatcg cttgttgcag cgccgaacga ttcgcctgat
      241 ccgcttcggc gggagtgaac gattctgcag catatgcctg tagatgcagt gccaccaggc
      301 cgtcatcttc acccagtttt ttttgccccg accagataac cgcaccggtt gccagtaatt
      361 catcgagcat ttctgacgaa tagtcgcgta cgcgggcagg caggatctgg ctttcccaga
      421 gtgaggcggg taaaccgact ccggcaagct gttcgatcac ccgcatcacg ccatctacgc
      481 cctcataaac gcctggcgat gttgaggcaa agagcgccgg gctaccatcg gtggcgggta
      541 atacgccctg acgttccagc agcaatcgcg cataggtcgt ggctgcaacg ggacgcgtcg
      601 cttctctggc ggcttgcagc gagcgcaaac gcagacgacg aaatacttca tcgctcaccc
      661 agatgtcttg ttgcagattc atcaccagac cctgttcacg cagttgctga agctgctctt
      721 cgacaatggc aataccgaga ctaaactcat gagccagttg ttcagccgtg accagagcat
      781 gagcccggag atagcgcaga aagaggtcgc gaagcgggta actgactcta tgtaaataaa
      841 tctctggcaa tgactctggt agtcgtacgc cgagggcatc acgcagcctg gcggcatcat
      901 ccatacaggc aagacgctcc tgtccgctaa tcatcgctgg gaagattcgt tttactgcaa
      961 gaagattttc cagatacgac gcaacctctt cactgctgcc tgtatgccgt tgcgccaggt
     1021 cttcaacggt cattggcccc agttcgcgca gcaggtcgaa caatccttct tcacctttcg
     1081 ctcttctgcc aggagccagt cgttgcaact cttcttccac ctggcgaatg acctgcgggt
     1141 cgagtaattc ccccggatcg acctgtccga gtagattgcg cagtaactcg ctgtccagcg
     1201 acagtacgga tgcccggcgc tctgccagcg gggcgtcgct ctggtacata aattccgcga
     1261 catagccgaa caataaactt gtggcgaaag gcgagggcgt agtggtcgtt acatcggata
     1321 tttgaatttc gccaccgttc aggcgacgca tcaaacgttc cagtgcggga agatcataaa
     1381 catcttgcag acattcgcgt aggatttcga gaatgaccgg aaaatctgga tatccctgag
     1441 cgatttccag caactgactg gcgcgcagcc gttgttgcca aagcggggtg cgatggcccg
     1501 gagtgcgccc cggcattaat aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa
     1561 gtgccgagct gcctaccgcc tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa
     1621 aaatcgcggc gtcgggcaat ttaccatcag tgtcaggaat gcgcgcaaca atgccgtcat
     1681 cactggcgac cacagacgcg tcagcgcccc atagggcatg tattcgcccg gcaatcgcca
     1741 gcgcccaggg ttcatgcacc cgtcttccat agggagagtg caaaataata cgccagtcgc
     1801 caatttcatc acggcaccgt tcgaggacca gatggcgact ccccggaacg atgcccgtcg
     1861 cgttgcgttg ctcgtcaatc aacccctgaa tattggcgtt cgtattttct tctgccagcc
     1921 acgggggaat agtcccggaa aagaacgcgc catccgccag taaatgaaga aaatcgccga
     1981 tcatctcgcc taattcagcc ggacgtccgt taccttcacc acgccagaag gggagccggg
     2041 cagaacgacc cggagcagga gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc
     2101 atgaggtagc gccgagagtg ataatgtcgt tcacccgcga ctcatatacc atctcctcat
     2161 ccagttcacc cacccgccgc gaaccggcct tttcttcacc ttcgggtaat aacacgctat
     2221 acatgccacg atccggaatg gtgccgccgc tggtaaccgc caacaattga gcgccaggtc
     2281 ggacggtcaa tatcccggtc tccctgttcc agaccagttt ggggcgaaaa gcagaaaaat
     2341 cgccagaggg atagcgcccg gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca
     2401 gatctttcca cggtgcggca cggcgtacgc gggagtacca ttcgtctacc tgtaatgcct
     2461 ccatcgccgc ggcggcaacg gtttgctgcg caaggacgtc gagtggatta tgcggcggtg
     2521 tcaggttttc cagcctgcct gcgaacatac actctacaat gactgcggaa tcgactaagt
     2581 cacgccgggt acgagggaaa aacagccctt tagatacacc gccaacctga tgtccggcgc
     2641 gaccaatgcg ttgtaaccca ctggcaacag aaagcggcgt tgccacctga atcaccagat
     2701 ccactgcgcc catatcaatc cccagttcga gactggaggt tgcgaccacg cagcgtaact
     2761 cacccgattt cagcgcctgc tcggtgattg ctcgttgttc tttagagacg gagccgtggt
     2821 gtgagcgggc aataaaaacg tcgctacttt gtacacggtt agaggttgcg ccggaggtcg
     2881 actcgaaatg ggccgcatca acggcgatag acggggaacg ctgtaagcgt gcggcgtaaa
     2941 gctcatttaa tcgtgccgtc agtttttccg ccagcccacg tgaattagta aagacaatgg
     3001 tcgagcgatg gcgcaacact tcatcaagga tacccgtttc aatatatggc cagatggagc
     3061 cttcccggcc ggcatggctg tcttcgccgg tgctgctggc gaccgatgag acatcatcca
     3121 tattggcgac cggtacgaca attcgtatct gcggatggcg cattgcgggc gggttgacta
     3181 cagtaaccgg gcgatcgcca ccaagaaagg ctgccacatc gctggctgag cgcacagtgg
     3241 cagaaaggcc aattcgctgt gctgaggtgt ggagcagcgc atcgagccgc tccagactta
     3301 acgccagatg cgcaccacgt ttactgcccg ctaccgcgtg gacttcatca ataattaccg
     3361 tttcgccgcg ccgccgccgt tcatcggcaa tacccttcaa cgggagctgc aagttgcgct
     3421 gaacgtcggt gcccagagct tttatcggtg aaatatagag gatgcgtgag gttttacgct
     3481 tatgcgcctc gcgggtatct tcgccgcctt cgcggaagag ccgatcgagg gcgtagagaa
     3541 atgctgccag cgttttcccg gagccggtcg gtgcaatcac cagcgcatgt tcgcttcgcg
     3601 ccgccacatg ccaggtttgc gactggacag cggtcggctg tttaaaggcg cgaagaaacc
     3661 agtcgcgggt cgccggtgaa aacacgtccg gcaggagcga tgaagggtct ggattatctg
     3721 ccattaacgc tattccccgt gaatatcatg caggatgcgt ggaatagcct cactatagat
     3781 gcctgaatca ttgcatgtca tcaggcatcg actcgattac acctcttcgg cggcaggtag
     3841 cggccagcct cccagacgtt tccagcggtt gacgatttca caaaacagca cagcagtgcg
     3901 ttcggtgtcg tacagcgcgg agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg
     3961 gcaagccttt gacaatacgg tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa
     4021 agtggcgaaa gggtggaacg ggttacgttt cagtgaggcg cgttctgcgg cggctatcat
     4081 aaagctgtga tcaaaattgg cattgtgcgc caccataatg gcgcggttac agccgctcgc
     4141 tttaatacct ttacgtacaa ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc
     4201 gccacgatcg ggatcgttcg ggtcaatgcc gttgaaggcg agggcttctg gttgcaaatt
     4261 tgcgccgaca aatggttcga cgtggaaatg taatgtggtg tccggcatca gccagccttg
     4321 ttcatccatt ttcagggtga tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa
     4381 tccggctgtt tcaacatcga tcacaacagg ataaaaacca cgaaaacggt cgcacagacc
     4441 ggtaagttga gcgttatcgg acatctgatt ctcttaatac ggataaattg cagcgcgcat
     4501 tatgacaaat attacttgtc gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc
     4561 aggagattag ttgcccagac cgcgaccggc gtctttctct tcgattaact caattttgta
     4621 accgtccgga tcttccataa acgcgataac cgtagtaccg ccttttaccg gacccgcttc
     4681 acgggtcacg ttacccccgt tttgacggat tttttcgcac gcttcagcgg cgttatctac
     4741 gctaagcgcg atgtgaccat aagcagtgcc gagttcgtat ttatccacgc cccagttgta
     4801 ggtcagttca atcaccgctt cttcggtttc cgggccgtag ccaacaaacg ccagtgagta
     4861 tttgtattcc gggttttcgc tggtacgcag cagtttcatg cccagcactt tggtataaaa
     4921 atcgatggag cgttgcaaat cgccaacgcg cagcatggta tgaagaagac gcatcttttt
     4981 atcctcaaaa tggctaacag attcaaaatg ttgttttagt atagcggcgg ctttacgccg
     5041 ctcgcaatgt tggattacaa cgtcgggtaa tcggtatagc cttccgcgcc gccaccgtag
     5101 aaactttcgg cacgctgtgg gttaagctca gctttgcgct gcaagcgggc gaccagatcc
     5161 gggttcgcaa tccagtcacg accaaatgcc accgcatcaa ttaacccttt gccgatcagc
     5221 gtttcagctt tttctactgt gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg
     5281 cgtacttttt cgcggaacgc atcagtatac ggttcacccc ccgcccaatc tggttctgac
     5341 atatgcagat aagcaatgcc gcgtttaccc agttgttcaa tcagatacag tgcatcggct
     5401 tcttcattcg ggccgttatc cgtgttctgg aaagtaccga ttggcgaaat gcgaatgcca
     5461 atgcgatcgg caccccattc ttcaatcccg gcatcgacca cttccagtac cagacgtgcg
     5521 cgattttcca cgctgccgcc gtactgatcg gtacgatggt ttgaagaagg agaaaggaac
     5581 tgatgcagca aataaccgtg agcagagagg agctctacca gatcaaaacc ggcttcacgc
     5641 gcgttagcaa tggcctgacg gaaatcattg acgatacctg gaatctcttc tagttcaagc
     5701 gcacgcggca tggatgtttc aacacggatc gcctgaccat tttcatcgcg cagagaagta
     5761 cgtgttcccg cgctaagtgc tgaaggcgct accggtgcct gaccgccagg ttgcaggctg
     5821 gcgtgagaaa tgcgtccggt gtgccacagc tacacggcca tatgaccatt ttcagcatga
     5881 acgccagcgg tgattttttt ccatgcggca atttgctccg gactatggat gccaggcgca
     5941 cctgcatatc cttttacctg ggcagaaatt tgcgtggctt cactaataat caaaccggca
     6001 ctggcacgtt ggcgatagta ttccgccatc aacggggtag gaatgtcacc cggttcaata
     6061 ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg ccgccgtgat cgcgcccact
     6121 ttcagtgggg aatacagttt ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa
     6181 aatgcctgct aaacggcagg cgtcgcaata atgtttttta catgggccag cgcgttttcc
     6241 agtggctcga aactgcgcga aattttggcc tgcagattcg cacccagcca tagtgcgtaa
     6301 agcacttgtg cctgttgtag cggttcgcca caaaaggtta aacaatggtt ctcacggcca
     6361 ttttccagcg cctgagagag cagggcgatc acgccgcgag cacctttatc catcgcgctg
     6421 cgcatatctt ctgacagatc gcacacttcg gcagagagtt ttactgtcag gcaaccactg
     6481 atggttccat gttggcaaaa ctggttcagt gtttgctggt aataagccag tatgcggtcg
     6541 cggtagttac cttcgccgga ttgcagcaac tcagtcagtc gctggtgata tgcggtgtaa
     6601 tgacgctcaa gcatggcaac gccaaacgct tctttagagc gaaagtagtg atagaaggac
     6661 cctttcggca cttcagcggt ttttagtaat tcgcttagcc ccatcccggt gaatccacgt
     6721 tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc gagtatcatg ttcggtgtgt
     6781 ttgttcatgc gttggagtgt agtagaccag tcggtctagt gcaagcaaag ttgctacttt
     6841 ggacattaac agcttcaata atggtaataa tcttatctga ggagggcgaa gtggcggagc
     6901 aattagagtt ctttcccgtc cagagcccgt gccggggaat ttgccagtct gacgaacgcg
     6961 ggttttgtcg cggctgtttt cgtagtcggg atgagcgttt taactggaat aaaatgagcg
     7021 atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg
     7081 caaataagcc agcgtcatca gacgaaccgg agcaaccatc actcttttga acctaatctt
     7141 gcgtatactc ctctcatatc tgataagagg aagcgattat ggttcagcgt attactattg
     7201 cgccgcaagg cccggagttt tcccgttttg tgatgggcta ctggcgattg atggactgga
     7261 atatgtccgc ccgccagctg gtcagtttta ttgaagagca tctggatctc ggcgtgacca
     7321 ccgtggacca tgctgatatt tatggtggct atcagtgcga agcggcgttt ggcgaggcac
     7381 tgaaactggc acctcacctg cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga
     7441 cgaccgcgcg tgaagaaaac gtcattggtc attacatcac tgaccgcgat cacatcatta
     7501 agagcgccga acagtcgcta attaatctcg cgaccgatca tctggatctg ctgttaatcc
     7561 accgaccaga cccgttaatg gatgccgatg aagtggcgga cgcgttcaaa catctgcatc
     7621 agagcggcaa agtgcgtcat tttggcgtat cgaactttac gcctgcgcaa tttgccctgt
     7681 tgcaatcacg tctgccgttt acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc
     7741 agccgttact gctggatggc acgctcgacc aactacaaca actgcgtgtt cgtccgatgg
     7801 cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg
     7861 atgaactggc tgtggtggca gaggagttaa acgcgggctc gattgaacag gtggtttacg
     7921 cctgggtatt acgtttacca tcgcagccgc tgccaattat cggttcaggt aaaattgagc
     7981 gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta
     8041 ttcgtaaagc ggcactgggg tacgacgtac cgtaagcact tttaggaata gccgccgttc
     8101 aaaaatgtgt cactggttta cacttattca ggaatgcaca atgaacggag gtcctatgaa
     8161 acgttttagt ctggctattc tggcgctggt tgttgcaacc ggcgcacaag ctgccagtga
     8221 aaaagtcgag atgaacctcg tcacgtcgca aggggtaggg cagtcaattg gtagcgtcac
     8281 cattactgaa accgataaag gtctggagtt ttcgcccgat ctgaaagcat taccccccgg
     8341 tgaacatggc ttccatattc atgccaaagg aagctgccag ccagccacca aagatggcaa
     8401 agccagcgcc gcggaatccg caggcgggca tcttgatcca caaaataccg gtaaacatga
     8461 agggccagaa ggtgccgggc atttaggcga tctgcctgca ctggtcgtca ataatgacgg
     8521 caaagctacc gatgccgtca tcacgcctcg tctgaaatca ctggatgaaa tcaaagacaa
     8581 agcgctgatg gtccacgttg gcggcgataa tatgtccgat caacctaaac cgctgggcgg
     8641 tggcggtgaa cgctatgcct gtggtgtaat taagtaatta agaggcctgc gtaccttgcg
     8701 gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg ccagacaatt gccgccagct
     8761 cacgggcggc aggttgatga tgacgggcaa ggctgtcgca aatccgctgt aattcttcaa
     8821 gtgtggccgc cagtgatttt tgctgaacgc cacgctcact catcactccc cgcaacagtg
     8881 aaatacagtt atcccgtact cgcgataacg gatcggaacg cgattcccag tcgcgcaatt
     8941 gccagacaac atgagaacag ttcagcagca ctacacccca gcgtaataac caacggcgag
     9001 ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg ataagtgagc gattcaaatt
     9061 cgctttcact cagtgttgga tggcggctta gctgatcgac aaaatcccgg cgcagcgcgc
     9121 gaatatggcg gcggctttta cgagcatccg atcctggacg cagaatggcg aacgctagcc
     9181 aagccagcgc aacgccaacg atttttgcca gattatcgtt aagaaagtca gcaaaatcat
     9241 acaccggggg attagtgaca gcgataaacg aacccataaa aacaatcagt tgcccccaca
     9301 gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga
     9361 gaaacagtaa aaattgccac agatcgctaa tctggaccat caggccaaat ttgaccacaa
     9421 agctgaatag cgaaagtaac accagcgtgc gcatcagcag tgacaacgac ttaaacggtg
     9481 ctgcgacggc ggagtagagt acgcagctaa ttgctgccag cgttaatgca ttggcaccgg
     9541 catcctattg cgatgcaata ctccatgcgc caatcatcat caacgtacaa aatgtacgca
     9601 gcccgctcca catagcttcg gcgttgtcgg tatgacgagc cagagcggat gtccgtgcga
     9661 gtctggcatg atcatctaca ccgctttgca gacgatgtaa ttcctgacta ctttgcagat
     9721 aaaggcggca aaaataacgt agtcgctgcc agaaggcgac gtgccggtag tcggcgacgt
     9781 tggtcgggcg tagcggggcg ataatacgtg cgacggtgta agcatctgtt tgcgaactgg
     9841 cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc acctggcggt gagggccagt
     9901 tgagcaacat acgtcgcagg ctggagatga cactggtcat acgtaattgc tggtggagta
     9961 gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg gctccagaaa gcctggatac
    10021 gcagcaaatt catggtcagt atctgcccaa tccccccctc atgtgctgca cgaatggcat
    10081 cgtttgtttc aggctgccag agtaaactgg catgttccag taatcgggcg tgcatgtttt
    10141 tcaatgcggt taaaagggct gtagcatcgg aactgctagg caggatcatc atcatcatgc
    10201 cgccgcacaa aataccgact atcacctcgc aaacgcgcgc ctgagcgata tcctacagct
    10261 ggctggcctc agtaatatta accatcggaa aggcgatgat ggcagccgtg tagccgccag
    10321 ttgaaatgca tacgcgacgt tattcgtgaa gtgcgcacag gcccaggtac aaaagccaag
    10381 ccacgccgac atgctcaata gaaaaaacca cggctcattg agcgtatgcc cggcaagaag
    10441 cagtgccgca atggctccca gtaaactgcc agcgatgcgt ccgaggcttt tgctgatgac
    10501 accgccaacg gtgggaaagc taaccactgc agccgaggtc atcgcccaat agggttcatc
    10561 cagattcaaa taataggcaa ccgtcagcgc cagacacatg gcaatggtat tgcgtaacgc
    10621 ataacgccat tgcgccagcg tggccttgaa ccagggcaaa ttgcgcaagg accatgacga
    10681 tgcgttcatt attgacctac ggcaatgcta caggtggtgc ccgacaccag cgtgacatcg
    10741 cctggaactt tatccagcgc aaagcggacg ggaacgcgtt gggcgagacg aacccagggg
    10801 acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctttg atcataaatc
    10861 gcccgaccga tactggaaac gtgaccccgt aacgttttgt tatcactata gagggtaatt
    10921 tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata accaatgacg
    10981 taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc gtaatcgccg
    11041 atgcgggtag tgaggttagt cacccaacca ctcaccgggg cgcgaatttc cgtttgcgcc
    11101 agttgccatt gtgtctgttt cagcgtggcc tgtgcggcat taacactggc ctgcatcgct
    11161 ttaacattga ggttagcggt atcgagttct tcggcagaaa taaaattttg tgagagatga
    11221 cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt cgccagctga
    11281 gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag cagaagatcc
    11341 cccgcgttaa ccagttggtt gtcttttata ttcagctcaa caatacgtcc cgacacctgt
    11401 ggggtgatag aaaccttttc tgcgcgtatt ttcccgtcgc gtgtccacgg agattgcatg
    11461 taataattcc acagccacca tccggcaaga accgcaacta tcgctacaat aattattgag
    11521 aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc agagcaaggc
    11581 aaacgcaaat cgcaaacagc gataaatcca tttacaaggg atgccagatg tcaccggcgt
    11641 agatccagcc acgaagcagg cggtgtacga caagccagat gacgaatcca aacgcgaagg
    11701 ctttgaaaaa cggaggaaaa tagacggacg caccgaacac cagatcttgt aagggcaatc
    11761 ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat atattgcgtt
    11821 agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa aaatatgtct
    11881 gacggtaacc aaatgcagca atacatttgt tttagcaata aaattgctgc acactattct
    11941 aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga atcgccacta
    12001 ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca tcgcctgaaa
    12061 ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca gttacctcca
    12121 gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc actggtccgt
    12181 actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc cagcgatcgt
    12241 cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga aatggaagct
    12301 gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga actggagcaa
    12361 ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc caaagggtga
    12421 aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag cgcggagaaa
    12481 cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag aaacgagtgt
    12541 tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt tccagctcga
    12601 caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca cctgcaacag
    12661 cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc cccaggaaac
    12721 caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg ccctgaatct
    12781 gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt ttcgcttcag
    12841 aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca agagacagac
    12901 ctaccattga aacaaccaat acgcgtttaa tcattgaaaa atctcctgtt caccatgaaa
    12961 cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca tggttacatg
    13021 ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata aacaaaacat
    13081 tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa tcggtcattc
    13141 cgcatggtat tcacgaaaag tttatgtttg agtggggcgc aaacgtcaga ttgaaaaagg
    13201 atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc ttgatggtgt
    13261 tgatgttgtg ttggcgacaa tagatgaaca ccgggtcgca cagctggcaa gcttgagctg
    13321 gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc agcagcttac
    13381 actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg atgcggtcaa
    13441 tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg gctgtcacgg
    13501 tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac actttacaga ttggcgataa
    13561 caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc gtcgcgatat
    13621 tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct tgctggctca
    13681 cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt cactgctcat
    13741 tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga tggatgcctg
    13801 gatctggcgt caggccggta aaccttacga taaagatgcc gagtgggcac gggcgggtaa
    13861 agttattctc ccactgctgc aaaatatgct cagcgaccca tatttctcgc aacctgcacc
    13921 gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt tacgccattt
    13981 tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg ccgtgaccat
    14041 ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg gtggaggtag
    14101 tcgtaatccg ctactcatgg cgcgtctggc ggcattactg ccaggcacag aagtcaccac
    14161 caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg cctggcttgc
    14221 ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg caagccagga
    14281 gacggtactg ggggctattt tccctgctaa cccgtgatat cagagttaac tgaatttttc
    14341 ttaccgcgtc tgccgttaca cttggaacgt tagggagggc gtattgccct ccagaccagg
    14401 aaagtcttcg ggaaatgtct atgaaaaaac tgttactgat ctgtttgccg gtgcttctca
    14461 ccggctgtag cgcgttcaat caactggttg agcacatgca aaccgctacg ctggaatacc
    14521 agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa gtcagttttg
    14581 tttacgataa tcaactactg catctcaaac agggcatttc agcgtctggt gcacgttaca
    14641 ctgacggaat ctatgttttc tggtcgaaag gcgatgaagc gactgtctat aaacgcgacc
    14701 gcatcgtatt gaataactgt cagttacaaa atccacagcg ttgagatttt tccaggggcg
    14761 gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga taacgacgaa
    14821 ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg ccgtcgcgat
    14881 cttcccgccg atccattaac cctttttgaa cgttggctct ctcaggcgtg tgaagccaaa
    14941 ctggcagacc ctaccgcgat ggtggtcgct accgtggatg aacatgctca gccttatcaa
    15001 cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac caacctcggt
    15061 agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt cccgtggcat
    15121 acccttgaac gccaggtgat ggtgatcggt aaagcagaac gactttcgac tctcgaagtg
    15181 atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt ttcgaagcag
    15241 tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct gaagcagaag
    15301 tttcaacagg gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt cagccttgaa
    15361 cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt gtaccagcga
    15421 gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa aatcttgctt
    15481 taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca tctggcgaaa
    15541 agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg gcaagcagta
    15601 acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac gaggaagcgt
    15661 tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat cctaccgctg
    15721 acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc caacaggcgg
    15781 gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac ccgagcttca
    15841 aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg gacaaaatcc
    15901 gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct atcgcggcga
    15961 acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat attggcaaac
    16021 acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc aaccgtgaag
    16081 atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat gacttcgcct
    16141 gtctgaacaa acagtacggt gtggtgctgc aaattggtgg ttctgaccag tggggtaaca
    16201 tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt ggcctgaccg
    16261 ttccgctgat cactaaagca gatggcacca aatttggtaa aactgaaggc ggcgcagtct
    16321 ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc aacactgcgg
    16381 atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa gagatcaacg
    16441 ccctggaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat gtactggcgg
    16501 agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa cgtattaccg
    16561 aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa cagctggcgc
    16621 aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag gcgctggtcg
    16681 attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc aatgccatca
    16741 ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa gatcgtctgt
    16801 ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt tgctggaaat
    16861 aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta tacagggaac
    16921 atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc gggtaacagt
    16981 gcggcagagt ttcctatgcg ccgcctgggc gcgaacgtct ggccgctgaa caccgttcaa
    17041 ttttctaatc acacccagta cggcaaatgg actggctgcg tgatgccgcc cagccattta
    17101 accgaaattg tgcaaggcat tgccgccatt gataaattac acacctgtga tgccgtgtta
    17161 agtggctatc tgggatcggc ggagcagggt gaacatatcc tcggtatcgt ccgtcaggtg
    17221 aaagccgcga atccgcaggc gaaatatttt tgcgatccgg taatgggtca tccggaaaaa
    17281 ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtgc ggcacggttt gcctgccagc
    17341 gatatcattg cgccgaatct ggttgagctg gaaatactct gtgagcatgc ggtaaataac
    17401 gtcgaagaag cggttctggc agcgcgcgaa ctcattgcgc aagggccaca aattgtgttg
    17461 gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt ttgaaatgct gctggtcacc
    17521 gccgatgaag cctggcatat cagccgtccg ctggtggatt ttggtatgcg ccagccggta
    17581 ggtgttggtg atgtgacgag cggtttactg ctggtgaaac tgcttcaggg ggcaacgctg
    17641 caggaggcgc tggaacatgt gaccgctgca gtctacgaaa tcatggtgac caccaaagca
    17701 atgcaggaat atgagctgca agtggtggct gctcaggatc gtattgccaa accagaacat
    17761 tacttcagcg caacaaagct ctgaaatatc tcatttcggc ccacaacgac ctgtgggccg
    17821 atggctatta ctttaagcct tccgctgaca gtgcgtcttg tacttccgga cgttcagcca
    17881 tacgttgcat aaatgctgca atgtgctcta acccttccag attcagtttc accgcgtatg
    17941 cccagcgcag aaccgtaaac agataggcat cagcaattgt aaatctttgc ccgcagatcc
    18001 agtgctcatc cttcagtgtc tcgttcacat attgcagctt cttctccagc tgcgcgcgaa
    18061 ctgtcggttt gtactcttcc ggtgtatcag ggcgaaacag aggtgtgaaa cctttatgca
    18121 gctcggtggc gatgtaattc agccattcga tggttttata gcgggaaata ctgtttaccg
    18181 gtgccagcaa ctggcggtcg gggacgctgt cggcaagata ctgcataatc gctacgcctt
    18241 ccgtcagcaa agtaccgtca tccagcagca atgcaggcac ctgcccctta gggttaacgg
    18301 caaagtaatc gtcaccgttt tcgagacgtt ttttcattaa atccacactg acgagggtaa
    18361 aatcctttcc gctctcacgc agggtgatat gggaagcgag agagcaggca cccggtttgt
    18421 agaacaattt catcggtaac tccttttgtc tgtggtttaa agtatggtag tgcgttgctg
    18481 aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta aaagagtttc cctaaatttt
    18541 acgctacagc agctttcgcc gctttgtctg cagcgtcatc ctgcgtcata cggtgcagtt
    18601 taggcgcggt cagcagcatc agtactgcaa taatggcagt agcgacacca atctgcaaga
    18661 atacgcgacc ataaacttcc agtgacatca gcgggtcggt aacgttatcc ggcacagcca
    18721 tcatacccgc aacataacca ccaatcaggt ttgcaccggc agtggtcagg aaccagctac
    18781 ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc aaccattgcc agacccagac
    18841 cagagatcat cagttccccg atgctctgca ggccatagct tgcgaccagc cagcttacag
    18901 acacgatacc agcgtcagac gcgaatttcg cacccagcgg cagaatcagg aacgcaccag
    18961 aacacatcac catgccgatt gcaaacttgg ttggcatcgg cagggtatcg cccatcttgt
    19021 tatagatagc ggccagaatc ggactaccga tgatgatcca gaacgggttc agtgcctgat
    19081 actgctcagg ttctacggcc agacccagaa tggagtgctc aacgttacga atcgcaaaga
    19141 agttcagtga cgttggcatc tggctgtaca gcacgaagaa gataatggct tcgagcatca
    19201 ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt catcgcgaag gcttctttac
    19261 cgaagataac cacgataccg aaggcaacaa cgcccagcgc catacgcgca acttcctgat
    19321 tgtgcagcag ccaggtggcg atagcgatca gtgccacaac accaataatg gtcagtagca
    19381 ggttacggta gttgataggc tcgaagtctg gttttgaacc gtactgttta acccagcgtt
    19441 ggcagaaggc gaagttaacg atagtgatca gcaggcctac aacgctcaac gcaaacgcaa
    19501 cactccagcc gtatttcgcg gccagccacg gcgtagcaat catagagaag aaagagccga
    19561 tgttgacgga catgtagtac atggtgaatg caccgtccag acgcgggtcg tttttctcat
    19621 agcatgtaga aagcagagaa gacgggttag ctttaaacag gccgttaccg accgcaatag
    19681 ccgccatacc catataaacg ataccggcgt cgtgaccaga ccaggcaacc agagcataac
    19741 caatcgccag cacaatagcg ccgagcataa ttacgcgttt agtacccagt accttgtcac
    19801 ctaaccagcc gccgatagcg accagaccat aaaccagggc actaaaggaa gagaaaaggg
    19861 tgattgaatc cgcttcagac atacccagtt gtttaaccag gtaaacagcc ataattcctt
    19921 gtaggccgta ataaccaaaa cgttcccata actcaatcga gaagatgaga tagaacgcct
    19981 tcggttgttt gaaagcgttc aaactgacgc tttcagttgg tttttggttt gcagtggaca
    20041 cttttacctc tttattttac atcccatatt aacgggggtg ctttctcgcg ttatgtctaa
    20101 aaaacatacg cgcaattgtt atagtgggag gggaaacggc aggtaatgtt cactatcttg
    20161 cactttcaga caagtccttt gtaataatct gttacatata actgaggcgg ggaattcaac
    20221 cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc agattttttc
    20281 actgcactga gaggggggtg atggcttaac ggcgatatgt tctgctattt agcgttttta
    20341 ctagtgagat agtccagttt ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt
    20401 gttgcgtgtg taaatttaaa tgattttaac ctgattcttg caatgttttt acattgcgac
    20461 agcatcgtga tcttgatcac gaatgaatag aaaatttcgc tattaaaaaa acaattaacc
    20521 tgcatttttg gttatccgca aacggattac ctgatgataa cgctaagcaa tggcatcatt
    20581 cacgttaaaa agatgtttta aaagaggata aagaaaggtt atcaatgggg taatcggtgt
    20641 tacccctttt cttcagatgt caactttctc tttgtattca caaagatctt caataataca
    20701 agagccacag cggggcttgc gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg
    20761 gtggcagtcg actttaaact ctgccggaac cactttcagt agcttttctt ctacctgttc
    20821 aacgtttttc cccggcgcaa attgagtacg attacaaacg cggaaaatgt gcgtgtcgac
    20881 agcaatagtc ggccagccga atgcagtgtt taatacgacg ttggctgttt tacgacctac
    20941 gccgggcagg gcttcaagcg cagcacgatc ttccggaacc tcgccattat gctgctccag
    21001 taagatacgg caggttttga tgatattttc tgctttgctg ttataaagcc caatcgtttt
    21061 gatataggtt ttaacccctt caacgcccag ttcaagcatc gctgcaggcg tattcgccac
    21121 cgggtagagt ttcgccgtcg ccttattaac actgacatcg gtcgcctgag cggaaagcag
    21181 tacggcaatc agcaattcaa aaggcgaact gaaattaagc tcggtggtgg gatgaggatt
    21241 gttctcacgc aggcgagtga ggatctccag gcgttttgct ttgttcatca gacattccct
    21301 gtttcaccgt ttggcaatgc acgttcggca gctgcttcag tacggcgctt tttcattctt
    21361 tcatcaatca ggtattttcc tgccagcatc agtcccaggc caataaatgc acctggtggc
    21421 agcatcgcca gcaggaaagg ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc
    21481 cagctaccta acagcgcatc tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt
    21541 agtgaaccca gcacgaacat ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt
    21601 gccgaaagcg ccggaccttt tttggcggcg aaggcttcag cgcggcccac aacgatacag
    21661 ttagtgacaa tcagcggaat aaaaatccct aatgattgat acaggccaaa ggcgtaggcg
    21721 ttgatcagca tctgtacagc gctgaccacc gaggcgatga tcatcacgta aatgggaatg
    21781 cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt
    21841 accagcgtag tcgcaagtcc taaacccaga gcattagtgg cagtggacgt gaccgccaac
    21901 agaggacaaa ggccgagcaa ctggaccagc gcagagttgt ttttccacaa cccctgaaca
    21961 ataacgtctt taatttcgct cacgctttat tctccacagg caggaagttg agaaagttgt
    22021 gccggtaacg tctgagcgta caatccggcg cgttttaccg cattaaccac cgcgcgggga
    22081 gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc
    22141 gcatcatccg caccactgat ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc
    22201 agttcgattt tatcgccaag ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt
    22261 accgtgccgt taaaatcggc tcccaccagc agctgaatcg caccggaata gccatctggc
    22321 gcggttgctt ccagaacggc ggctaccggt ttgtcatcct gtttggcgat gtaaacccga
    22381 tgctcacctt tacctaactc tggcgcagtt accagatagc aactctgtgc cagcgcattg
    22441 ttatagcgtt cggctggcag cacctgatca aataatgcct tttgttgcag acttgcctgt
    22501 tcagcaatcg tcgttttggt catctggttg atggccgcag ttaaccctgt tgaacccgct
    22561 gcaaatagcg ctaacgtaat gccgtgtttt cggatagttt tcagcatggt ttaccctttg
    22621 cgatggccgt agacgcgcgg acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc
    22681 agcaggacgg caaaagccac gccgtcagga tagccgccga aactgcggat caaccagact
    22741 aataagcccg caagcgcgcc gaaaataaga cgaccacgat tggtcgtaga agcggtaacc
    22801 gggtcagtca aaataaagaa tgcgccgagc atggtcgcac cagacagcag atgaatttgc
    22861 ggtgctgcca gtgtttctgg tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt
    22921 actaagaagc tgaggggaat atgccagcga atcgctttct gccatagcaa ccacaggccg
    22981 ccagccagcc aggcgagatt tacccattgc caaccagcgc ccgccagaat accgctgtag
    23041 atcggatatt gcataatctg ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta
    23101 tccagcggtg tcgcctgact aatgccatca atacctaagt gtagtgtgtt catatcagca
    23161 ccactggcgg tatgcccgct gaaaataacc tgtatggcgt cgataaaacc agggatgttg
    23221 accgcaattt catgtggcgg taaccagctg gtcatctgca ccgggaagga gatcagtaag
    23281 accacataac caatcattgc cggattaaac gggttttgcc ccagaccgcc atacaactgt
    23341 ttagcgatga tcaccgcaaa caccgtaccc agcacgacca tccaccatgg cgcgaggggg
    23401 ggaatactta ccgccagcaa taagcctgtc agcaatgctg agttatcttt caacgttgcg
    23461 gctaccgact gcttgcgtag tttgagtacg agagcttcgg ctaacagagt actaaccgat
    23521 gccaacagga tctgaacgag agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct
    23581 ggcacggctg cgagcaacac caacagcata atgcgcgatg tctggcgctg gttatgggta
    23641 taaggggagc tagctattct gaataccatt tagtcctcgt ttacaacctt ttgctgggcg
    23701 gcttttttgg cctgagcgcg ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc
    23761 tgttcttctg gttctgcgtt agtctgttgc tgttccagct tgcgcgcttt ggcacgggca
    23821 atagcggctt cgacggcggc tttgcgcgga tcaacctgtt cttcaggtac cgcattagcc
    23881 tgttgctgtt ccagcttgcg tgctttggcg cgtgcaatag ccgcttcgac ggcagctttg
    23941 cgcggatcaa cctgttcttc tggctccgca ttagcctgtt gctgttccag tttgcgcgct
    24001 ttggctcggg caatagcggc ttcgatggcg gctttgcgcg gatcaacctg ttcttctggt
    24061 tccgcattag cctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg
    24121 acggcggctt tgcgcggatc gacctgttct tctggttccg cattagcctg ttgctgttcc
    24181 agcttgcgcg ctttggcgcg ggcgatagct gcttcaacgg cagttttacg tggatcagca
    24241 acggttgctg cgtcgttagt ttgctgcagt tctgcctgtt tcgctctggc ttgcgcttta
    24301 cgggcttccc gtgctgcaat aattgcactg ttatccgggc gttcgcccgc tttaatcaca
    24361 ataggctgtg tagcctgggc ctgtttctct ttcacccgcg ccagagcggc agcaatcgca
    24421 tctttatctt tggctgcagg ttgaacggct gcgctcttat gtcgttcaag gcgagccgct
    24481 ttttcgcgct ccagacgagc ctggcgcgct tcgaaacgcg ctttggcttc tgcggcgcgc
    24541 ttttcttcct gacgaatagc cgcaatttca gctttttcct gacggaaata ttgcaccagg
    24601 ggaatattgc tcgggcaaac ccacgcgcaa gccccacatt caatgcaatc agcaatgtta
    24661 tgcgtggtag ctttatcgtg ttgctgacct ttgctgaacc agtacaactg ttgcggcaaa
    24721 agatccgcag ggcaggcgtc agcacaggcg ctacaccgga tgcagctttg ttcttcctgt
    24781 ggttcgccaa gttcattggc agagggagcc aacagacagt tggtaatctt tacgaccggg
    24841 acatccagcc atggcaaggt aaagcccatt agcgggccac ccataatcac catttgatcg
    24901 gcagaggggc agaatcgggc atcattcaat aaatgacgca ctggcgtccc cagccgtgcc
    24961 cagacgttgc ccgggcgagc gattgcttcg ccagtcaggg ttacaacacg ctcggtaatc
    25021 ggctcgccat caataacggc acgtttcact gcataagcag tgccgacgtt ttgcattaat
    25081 acgccgatat cggatgaacg cccgccatgt ggaacctgct tcccggtcag aatgtaggtt
    25141 aattgtttag caccgccaga aggatatttg gttggaatca cccgcagaga aatatcgtta
    25201 gagtccgcca gcaccgcgcg cagcatggaa atcgcctgcg gtttgttatc ttcaatgccg
    25261 ataagaattt cgcgtggctg cagaatatgc gcaagaatgc gaataccctc tacgacctga
    25321 gccgcgcaat cctgcatcaa acggtcatcg gcggtaatgt acggctcgca ctcagccgcg
    25381 ttgataatca acgtttcaat cttatctccg ccaccctgca atttaacgcc tgtcgggaat
    25441 cctgcaccgc ccagcccggc aacaccaaac tgatgtatgc gctcgattaa ctcttcgcga
    25501 ctacgagtgc gataatcggc ccagccgtcg cgcgggatcc agcagtcttc accatcggca
    25561 tcaataatca cgcttaattc agctaaagct gaaggatgag ccgtagagtg gggcgcaata
    25621 gccgtaacgg tacccgaggt gggcgcgtga acaggcagca ttttgccgcg accacgggta
    25681 agcggctggc cgcgcaatac tttatcgccg acgctaacgc acaactcacc ttcagcgcca
    25741 atatgctgtt tcagtggaat aacaaaacgc tgcgccagtg gtacctggcg caggggggta
    25801 ccgttggact gggttttcat ctccggtgga tggatgccgc cgttgaaatc ccagatttta
    25861 ttttttctga atgcagagaa taacttaagc atggtgttcc acgggaatga tacgcacggg
    25921 aatggtgttc agatcccatt tccaggagtc aggtgtttct gcgaccggtt gcaacgagat
    25981 gcagtgcgtc gggcacggat caacacataa attgcagccc gtacagagat cactcattac
    26041 cgtatgcatg gcacgggtag cgccaacgat ggcgtctacc ggacaagcct gaatacattt
    26101 agtgcagcca atacagttat tttcatcaat aaccgccacc atccgcgcag gcgttagctc
    26161 ttgcgcttcg ccatccagcg gctgcggctc gacattaagc aactcggcaa tttttagcat
    26221 cacagcttcg ccacctgggg cgcaacggtt gattttttca ccgttacagc tgatggcttc
    26281 cgcgtagggg cgacagccgg gataaccgca ctgaccacac tggctctgcg gtaagatttc
    26341 gtcaattttc tcaacgaccg gatcgtcttc caccgcaaaa cggcgggagg cataacccag
    26401 aatggcgcca aacgccaggc ccagcaggct cacggcggca acggcaatcc agatagcatt
    26461 cattacaact tcaccaaacc actaaagccc ataaaggcca gagacataag acctgcggta
    26521 attaacgcaa tggcattacc gcgaaaaggt gccgggacat cagccacagc aaggcgttcg
    26581 cggatggcgg tgaagagcac catcaccagc gagaaaccga cagcggcgga aaaaccgtac
    26641 agcgccgact gcaagaaatt gtgcccgaga ttgatattca gcaacgccac gccgagcact
    26701 gcacagttgg tggtgataag cggcaaaaaa atccccagca gacggtaaag caccgggctg
    26761 gttttgcgca ccaccatctc ggtgaactgc acgaccacag caatcaccag aataaatgcc
    26821 agggtgcgca ggtaaatcag attaagtggg atcaaaatcc acgtatcgat aagccaggcg
    26881 caaatagacg ccagcgtcat cacaaacgtt gttgccagcc ccatgcccat cgcggtttcc
    26941 agttttttgg aaacccccat aaacggacag agaccgagaa acttgaccag cacaaagtta
    27001 ttgaccagta tagttccgac aaacagtaac aggtagtcag tcatttttcg gcctgaaata
    27061 aaaaagccgc ctattatccg ttaatccaga gcaggcgaca acaggttaac tgtaaggtta
    27121 ttacggattc acaaacgtcg cttttacccg cgacgagcgt ttgaaatagg agacgatcaa
    27181 tgcggttgcc aacaaagtaa acagcaactg gcgaacggct atgccatctt ccacgggtga
    27241 aaaagcgaat gcttttactg ccagcaatac ggaaataagc agccagataa tatagtgttt
    27301 aggaacacaa cgccgacgtt taaagaaagc gatggtcaac cacagtgtgt aataccacat
    27361 ggcgatagcg gtaataaaag aaacgcccca taagatttgt gtcgtcagtg cttgtccgcc
    27421 gagcgtttga aatgtttgcg gagaagataa cgcagcggta tacagcaaca acgccagcgt
    27481 cgtactcaac agagcgacta aaagccaggc caacgggcca agcaaccagc ctccaatacg
    27541 ttgtggcgtt gtggtggtca taggttctcc agaatagcga cgcaaacagg caaatctaat
    27601 tttaggggcg ttattataag gcttttataa ccgatcgcta ctttctttat tggacatagt
    27661 gccagacgga cttcggtacc tggccgaggt caaacaaacg cccgccagaa accagctctg
    27721 cgcgacggtg atcggcagca cgatacatat tgatcagttc ctgatcgtcc gtcagggtgt
    27781 aattaagatg gtcgtagagt ttttccagac tttcgagtga actgattttg cgaaatttta
    27841 ataagtagtc ctgaacagtc ataaaaggtc cataaaataa ttaataccaa aggggtaggt
    27901 attattagtg atgataaaat ttcaccaaaa gtgaacgctt gtgttattac aataataaga
    27961 atgcgctgat ggcaaatatt cagggatgaa aaactgctct gaaaagagta acgcgcttta
    28021 ctatccctga caatcaccaa caacatcgaa caagataata aattcctggt ttaatatccg
    28081 acaagtgaaa acatgcaacc ggacgggcag catggcgctc tacaagcgca gagcttactt
    28141 gtgttgtacc gaagcactct gttcaggtgg ctgatagttg tcaatgtgac tcgccacgcc
    28201 aagaagaatg actgaaacga caagaacgat ccaacctgtt aattcaataa gacgattcat
    28261 tacagcccac atctctcttg attgatccat taacttcagg gggtaaatgt tacttagcaa
    28321 taatagctcc agcaagattt ttactgaggt ttttgcgata tttagctttt gtcgttggaa
    28381 aattcgctat ttttttgact taatattaaa ctgttgagcg tgggtaaagg atagtgtcaa
    28441 atagcaatca tacttcaatg agaggcaatg acatgagcga caacatccgt gttgggttga
    28501 ttgggtatgg ttatgcgagc aaaaccttcc atgcgcccct gattgcgggc acgcccgggc
    28561 tggaactggc ggtaatctcc agcagtgatg aaacaaaagt aaaagccgac tggccaacgg
    28621 ttacggttgt ctctgagccg aagcatctgt ttaacgatcc caacatagac ctgattgtca
    28681 ttcctacacc caacgatacc catttcccgt tagccaaagc ggcgcttgag gcgggtaaac
    28741 atgtggtcgt tgataaaccc tttaccgtga cactgtcaca agcgcgagag ctggatgcgc
    28801 tggcaaaaag cctggggcgt gtgctgtctg tattccataa ccgtcgttgg gatagcgatt
    28861 tcttgacgct aaaaggtttg ctcgcggaag gcgtgctggg tgaagttgct tactttgagt
    28921 ctcattttga ccgcttccgt ccgcaggtgc gcgatcgttg gcgtgaacag ggcggcccag
    28981 gcagcggtat ctggtacgat ttagcaccgc atcttcttga tcaggccatt acgctatttg
    29041 gtttaccggt cagcatgacg gtagatttgg cacagttacg gcccggagcg cagtcgaccg
    29101 attatttcca cgccatcttg tcctatccac agcggcgagt cattttacac ggtaccatgc
    29161 tggcagccgc tgagtcagca cggtatatcg tgcatggatc ccgaggcagt tatgtgaaat
    29221 atggcctcga tccacaggaa gaacgtctga aaaatggcga gcgtctgccg caggaagact
    29281 ggggctacga tatgcgtgat ggcgtactta cccgagtgga aggtgaggaa cgtgtcgaag
    29341 aaacgctgtt gacggtacct gggaattatc cggcttacta tgcggctatt cgtgatgcgt
    29401 taaatggcga tggtgaaaat ccggttccgg caagccaggc aatccaggta atggagttga
    29461 ttgagctggg catcgaatcc gccaaacatc gcgcgacttt gtgccttgca tgaaaaatgg
    29521 atggtgcaat ttgcaccatc cattttgtta cttcgcggcg actttttctc gcagtgcgcg
    29581 tttttcctca gcgctgagga aagccatttc cagaccatta atctgtgcct ggcggatttg
    29641 ctcgcgggat aacccagcag ctggcgcggc aacggtatat tcgtgaatga tatccactcc
    29701 ctgtacgccg ggatcgtcag tgttaatgcc ggcacgaatg ccatgctcaa ggaacgtttt
    29761 cagcggatgt gcagccagct ctgctacggt gctggtctga atattggagg tcagacagga
    29821 ttcaatacca atttgttgct cggcgagaaa atccatcagc gcccgatctt caatggcttt
    29881 tacgccatgt ccaatacgct ccgcacccag ttcacgaatc gcctgccaga tgctttccgg
    29941 cccggcagct tcgcctgcat ggacggtaat atgccagccc gcatcacgcg caaggttgaa
    30001 gtgagaaagg aacagacttc ccgggaaacc aagttcatca ccggctaaat caagggcggt
    30061 aatctggtca cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg cttcgccgaa
    30121 ggttcggctc ataatgccga taagcttcgc ctgcacacca aaagtgcggc aaccttcacg
    30181 tacgccatcg atcaccgctt cgacaacacc cgctacaggc agcttatgtg ccattgccat
    30241 gtagcctggt gaaaaacgca gctcgacata gtgcaggccg ttacgggctg catcttcaat
    30301 gttttcaaat gccacgcggc ggcaggcatc aagagaggcg agaactttaa cgccccagtc
    30361 aagtttggtc agaaagctca ccagatcggg ttcgttggca atgacctgaa cgtggggaat
    30421 cagtgtttcc agggattgtg caggaagcga gatattatac tggcggccaa gttcaagaat
    30481 ggtctgggga cgaatgttgc catcaaggtg gcgatggata tcagttaatg gcagggtggt
    30541 atcaatcatg gtcgcactct ttttctggat ataaaagtgc gcaccattat aaaaacaaag
    30601 cagggtaaaa atccacgatt gcgcaacgat ttttacgttg cgcaattggg gttaacgaac
    30661 agcgcggatg gcgttaatta atccagccac acctttttcc agtttcgaac gtgggcagcc
    30721 ggcattgaga cggacaaaac cacgaccttc ttcaccgtag gtataccccg gcatgatcgc
    30781 gactttttct tgttcgataa gtgctttttg caacgcgttg tcgtcaatat tcaacggacg
    30841 taaatcaagc catgccagat aagtggattg tgggatctgc cagttgagtt caggaaacgc
    30901 ggcgttcatt ttatctgcga tatacgtcag gttatctttc agatagatgc gtaaggcatc
    30961 cagccacggc gcgccttgct gataggcggc gatatgggca gttaacgcca gtaccgaagg
    31021 ggaagaaagc ccatcacggc ctttcagtgc cgataaatag gcatcgcggc tactgctatt
    31081 ttctataatc ccgtaagcac cggtcagggc gggaatattg aaacttttcg agcccgacgt
    31141 tagcaacgcc cagtctccgc gagccacatt actccaggga atatgcggct gctcgcccca
    31201 aaccatatcc atatggattt catcggaaat aacccgcaca ccatgacgct cgcacaggtc
    31261 agccatgatc tccagctcat cgcacgtcca cactttcccg gtaggattct gtgggctaca
    31321 caggagcata attttacatt ctggtttcgc caacacggct tccaacttgc ccatattgca
    31381 aaaccaacca tcagcctgct tctctaaagc aacgggcatt actgtgcgct ggttaccttc
    31441 aatggccttg taaaatgcgt cataggcggg tgtgtggatc accacgcctt cacctgtttc
    31501 agaccactga cgaatcagtt ctgaaaccat atagatgaca gaagggccat acaccaccgt
    31561 ctgagaatcg atggcggtgt aatgctgggt ggaaaaccag tgggcaatag cagcgagaaa
    31621 ctcatcgttt ttccagcggc tgtagccaaa tacgccgtgc atcaggcgct gattcagcgc
    31681 ctctataatg cagggggcag tggcaaaatc catgtctgaa atcgtgaacg gtaacaggtc
    31741 agcagtgccg aaacggtcag cgacataatc ccactgtgta caccatgtgc catgacgatc
    31801 cacgaccttt gaaaaatcga acatatctta tccttatgcc tggacagtat gcatcagacc
    31861 ggccatttca tcttttactg actgaacttg tggcccgata acaacctgca ggttatgttg
    31921 attaagttgt actacgccaa ttgcccgatt gtccttcagt gcctgcacat taacaagcga
    31981 catatctttc acagacaaac gcagacgggt aatgcagtta tcgaggctga caatattgtc
    32041 ggcaccgcct aatgcttcga ggattgcagg aacgttgtaa cctgatttac ccggcgcagc
    32101 ggcaacggct ttttcgattg agctggcaac ttcgctatcg cgccccgggg ttttgagatt
    32161 gaagcgggtg atagcgaaac ggaagatggc gtagtaaacg acaaaccaga ttgccgccac
    32221 cactggcacc atgtaccact tggttgacag accatgcaaa ataccgaaca ccacgaagtc
    32281 gatgatattg ccgtcggtat taccgatggt gacgccgagc acagacatga cggtgaagcc
    32341 gaggccggtt aacagcgcgt ggatgacata cagaactggc gctacgaaca ggaacaggaa
    32401 ttccagcggt tcggtagtgc cgccaacgac gcaggcgatc aggccagaaa tcagcagacc
    32461 tttaatttta tggcgatttt ccgggcgcgc gcagtgatac atagctaacg ctgcagctgg
    32521 cagaccgccg agaaacgcag gcattttacc ttgcgaaagg aaacgcgtgg cgctttcaga
    32581 aaaaccgtga gtggtcgggc aactcaattg cgtctggaag atggtcagtg cgccgctgac
    32641 ggtttgaccg cagacttcct gcgtgccgcc tgcgtcggta aagcgaatta atgccaccag
    32701 aatgtgatgc agaccaaacg gcaacagcag acgttcaccg gtaccaaaca gcatcggtcc
    32761 gaaatcaccc gcgctgttta tcatatggcc caagccgcta atacccatgg cgaaaatcgg
    32821 ccagactaat ggaatcacca ggccgacaag gcccatcacc agcgaggaga taattggtac
    32881 gaagcgcgta ccgccgaaga atgccagcgc atccggcagg cggatattat ggaaacgctc
    32941 atgcagcatc cagacgataa taccggcgat caccgcaccg aggatcccgg tatcgatcga
    33001 ctggatccca agaatgctct ggatgttatt ggctttcaga accgcggcat ccgtggttgg
    33061 cagaatgcct ttattggtca accagaagtt taccgcgagg ttcattaccg cataaccgac
    33121 gaagccagcg aatgccgcta cgcctttatt ttcgcgtgcc aggcccagcg ggatggcgat
    33181 acagaacatg acaggcagga aactaaaagc aaacgagcca atcttactca tccaggtaaa
    33241 gatagcctgc aacacggggt tgcccaggac cgggatcagg gttatgacat catggctgct
    33301 aagagaacta ccaatgccga gcataatgcc gcagaacgac aataatgcca cgggtaacat
    33361 gaaggttttg cctaactgct ggaagaactc ccacagcgtg actttcggtg ctgttttcgc
    33421 cgtcataaaa cgactcctcg tagagaacaa tctggattca gtaaattgcg cgagaagata
    33481 aaacgtttta tcaaatttta gtgaggcata aatcacatta cgcaacgata atagcgggta
    33541 taagataaat aaaaggtaaa acgttttatc tgtcacataa tcagggagta ggtcatctgc
    33601 atggctaccg ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg cgtgtcggta
    33661 agtaccgttt cgctggtgct tagtggcaaa gggcgaatct ctaccgccac aggagaacgc
    33721 gttaacgccg ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc ggcgctgcgc
    33781 ggcgggcaaa gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc gttttacgcc
    33841 gaattgacgg ccggattgac ggaagctctg gaagcgcagg gacggatggt ttttttgctt
    33901 cacggcggta aagacggcga gcagctggca cagcggtttt cactgttact gaatcagggg
    33961 gtcgatggtg tggtaattgc cggggctgca ggaagcagcg atgacctgcg acggatggca
    34021 gaagaaaaag ctatcccggt gattttcgct tcccgtgcca gttatcttga tgatgttgat
    34081 acggttcgcc cggacaacat gcaggctgca cagttgttga cggagcatct cattcgcaat
    34141 gggcatcagc ggatcgcctg gctgggaggg caaagttcct cattaacccg ggcagaacgg
    34201 gtgggaggct attgtgcaac tctactaaaa tttggtctgc cgtttcacag cgattgggtg
    34261 ctggagtgca catccagcca gaagcaagct gcggaagcta tcacggcgct tttacgtcat
    34321 aacccgacca tcagtgctgt ggtttgctat aacgaaacta ttgcgatggg ggcatggttt
    34381 ggtttgctga aagcagggcg gcaaagcggg gaaagcggag tcgatcgtta ctttgagcaa
    34441 caagtttcgc tggcggcatt taccgatgcg acaccaacca cacttgatga tatacccgtt
    34501 acctgggcca gcacgcctgc gcgggaactt ggtaccacac ttgcggatcg catgatgcaa
    34561 aaaatcaccc atgaagagac gcattcacgc aatcttatta ttcccgcccg gctcattgca
    34621 gcgaaataat cctctcttta tctgctatac ctggtagtgt cctttcctca aggttaatga
    34681 taaaaaatgt
//
LOCUS       DAJVTY010000023        34145 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVTY010000023 DAJVTY010000000
VERSION     DAJVTY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34145
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.023"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..318)
                     /locus_tag="OQQ66_001021"
     CDS             complement(<1..318)
                     /locus_tag="OQQ66_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1904029.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSV"
     gene            1672..2451
                     /gene="ipaJ"
                     /locus_tag="OQQ66_001022"
     CDS             1672..2451
                     /gene="ipaJ"
                     /locus_tag="OQQ66_001022"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCS1904030.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3396..4325)
                     /gene="virB"
                     /locus_tag="OQQ66_001023"
     CDS             complement(3396..4325)
                     /gene="virB"
                     /locus_tag="OQQ66_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCS1904031.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4542..4778)
                     /locus_tag="OQQ66_001024"
     CDS             complement(4542..4778)
                     /locus_tag="OQQ66_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1904032.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4782..6683)
                     /gene="ipaA"
                     /locus_tag="OQQ66_001025"
     CDS             complement(4782..6683)
                     /gene="ipaA"
                     /locus_tag="OQQ66_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCS1904033.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6692..7690)
                     /gene="ipaD"
                     /locus_tag="OQQ66_001026"
     CDS             complement(6692..7690)
                     /gene="ipaD"
                     /locus_tag="OQQ66_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCS1904034.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7741..8832)
                     /gene="ipaC"
                     /locus_tag="OQQ66_001027"
     CDS             complement(7741..8832)
                     /gene="ipaC"
                     /locus_tag="OQQ66_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCS1904035.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8852..10594)
                     /gene="ipaB"
                     /locus_tag="OQQ66_001028"
     CDS             complement(8852..10594)
                     /gene="ipaB"
                     /locus_tag="OQQ66_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCS1904036.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10600..11067)
                     /gene="ipgC"
                     /locus_tag="OQQ66_001029"
     CDS             complement(10600..11067)
                     /gene="ipgC"
                     /locus_tag="OQQ66_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCS1904037.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11125..11751)
                     /gene="ipgB1"
                     /locus_tag="OQQ66_001030"
     CDS             complement(11125..11751)
                     /gene="ipgB1"
                     /locus_tag="OQQ66_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCS1904038.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11767..12156)
                     /gene="ipgA"
                     /locus_tag="OQQ66_001031"
     CDS             complement(11767..12156)
                     /gene="ipgA"
                     /locus_tag="OQQ66_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCS1904039.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12169..13653)
                     /gene="icsB"
                     /locus_tag="OQQ66_001032"
     CDS             complement(12169..13653)
                     /gene="icsB"
                     /locus_tag="OQQ66_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCS1904040.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13967..15583
                     /locus_tag="OQQ66_001033"
     CDS             13967..15583
                     /locus_tag="OQQ66_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCS1904041.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15593..15955
                     /gene="ipgE"
                     /locus_tag="OQQ66_001034"
     CDS             15593..15955
                     /gene="ipgE"
                     /locus_tag="OQQ66_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCS1904042.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15955..16413
                     /gene="ipgF"
                     /locus_tag="OQQ66_001035"
     CDS             15955..16413
                     /gene="ipgF"
                     /locus_tag="OQQ66_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCS1904043.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16422..17537
                     /gene="mxiG"
                     /locus_tag="OQQ66_001036"
     CDS             16422..17537
                     /gene="mxiG"
                     /locus_tag="OQQ66_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCS1904044.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17545..17796
                     /locus_tag="OQQ66_001037"
     CDS             17545..17796
                     /locus_tag="OQQ66_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCS1904045.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17809..18102
                     /gene="mxiI"
                     /locus_tag="OQQ66_001038"
     CDS             17809..18102
                     /gene="mxiI"
                     /locus_tag="OQQ66_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCS1904046.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18108..18833
                     /gene="mxiJ"
                     /locus_tag="OQQ66_001039"
     CDS             18108..18833
                     /gene="mxiJ"
                     /locus_tag="OQQ66_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCS1904047.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGGFKRNKI"
     gene            18830..19357
                     /locus_tag="OQQ66_001040"
     CDS             18830..19357
                     /locus_tag="OQQ66_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCS1904048.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19299..19994
                     /gene="mxiN"
                     /locus_tag="OQQ66_001041"
     CDS             19299..19994
                     /gene="mxiN"
                     /locus_tag="OQQ66_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCS1904049.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19987..20394
                     /gene="mxiM"
                     /locus_tag="OQQ66_001042"
     CDS             19987..20394
                     /gene="mxiM"
                     /locus_tag="OQQ66_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCS1904050.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20378..20806
                     /gene="mxiM"
                     /locus_tag="OQQ66_001043"
     CDS             20378..20806
                     /gene="mxiM"
                     /locus_tag="OQQ66_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCS1904051.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20936..21568
                     /gene="mxiE"
                     /locus_tag="OQQ66_001044"
     CDS             20936..21568
                     /gene="mxiE"
                     /locus_tag="OQQ66_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCS1904052.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21555..23255
                     /gene="mxiD"
                     /locus_tag="OQQ66_001045"
     CDS             21555..23255
                     /gene="mxiD"
                     /locus_tag="OQQ66_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCS1904053.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23274..24341
                     /gene="mxiC"
                     /locus_tag="OQQ66_001046"
     CDS             23274..24341
                     /gene="mxiC"
                     /locus_tag="OQQ66_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCS1904054.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24354..26414
                     /gene="mxiA"
                     /locus_tag="OQQ66_001047"
                     /gene_synonym="virH"
     CDS             24354..26414
                     /gene="mxiA"
                     /locus_tag="OQQ66_001047"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCS1904055.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26427..26828
                     /gene="spa15"
                     /locus_tag="OQQ66_001048"
                     /gene_synonym="spaK"
     CDS             26427..26828
                     /gene="spa15"
                     /locus_tag="OQQ66_001048"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCS1904056.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26832..28124
                     /gene="spaL"
                     /locus_tag="OQQ66_001049"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26832..28124
                     /gene="spaL"
                     /locus_tag="OQQ66_001049"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCS1904057.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            28217..28555
                     /gene="spa13"
                     /locus_tag="OQQ66_001050"
                     /gene_synonym="spaM"
     CDS             28217..28555
                     /gene="spa13"
                     /locus_tag="OQQ66_001050"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCS1904058.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28542..29420
                     /gene="spa32"
                     /locus_tag="OQQ66_001051"
     CDS             28542..29420
                     /gene="spa32"
                     /locus_tag="OQQ66_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCS1904059.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29414..30295
                     /gene="spa33"
                     /locus_tag="OQQ66_001052"
                     /gene_synonym="spaO"
     CDS             29414..30295
                     /gene="spa33"
                     /locus_tag="OQQ66_001052"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCS1904060.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30300..30950
                     /gene="spa24"
                     /locus_tag="OQQ66_001053"
                     /gene_synonym="spaP"
     CDS             30300..30950
                     /gene="spa24"
                     /locus_tag="OQQ66_001053"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCS1904061.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30965..31225
                     /gene="spaQ"
                     /locus_tag="OQQ66_001054"
     CDS             30965..31225
                     /gene="spaQ"
                     /locus_tag="OQQ66_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCS1904062.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31225..31995
                     /gene="spa29"
                     /locus_tag="OQQ66_001055"
                     /gene_synonym="spaR"
     CDS             31225..31995
                     /gene="spa29"
                     /locus_tag="OQQ66_001055"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCS1904063.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32002..33030
                     /gene="spa40"
                     /locus_tag="OQQ66_001056"
                     /gene_synonym="spaS"
     CDS             32002..33030
                     /gene="spa40"
                     /locus_tag="OQQ66_001056"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCS1904064.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33043..33285
                     /locus_tag="OQQ66_001057"
     CDS             33043..33285
                     /locus_tag="OQQ66_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904065.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33892..34145)
                     /locus_tag="OQQ66_001058"
                     /pseudo
     CDS             complement(33892..34145)
                     /locus_tag="OQQ66_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT        10622 a         5014 c         6854 g        11655 t
ORIGIN      
        1 aaccgagaga gaacgaatga atgccctgag aatggtcatt ccgccacgat atccctggtc
       61 tctgatctcg cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg
      121 ttgacgaata taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata
      181 ttttggcggc tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag
      241 ttctctggca atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac
      301 tgtctcaaaa gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg
      361 atttcaggcg ttgggtgtgg atcactattg caccgttcgt gacacttgtt gactgcctgt
      421 gcggcatctg gtatcaaggt ggcgtgaagc cggttcaggt tctcatgcgg aacgttggaa
      481 cctatatccc cccgatacca aaggcgaaat gtaagtgaca aaatgcaatg aacacgttct
      541 ccgttaactg aggagcatgg tgataaataa aggtaaatgc agaaggtgta gagtctcgca
      601 gatttctgac atcatagagc ctaacattag atgcataacg ctacagattg tagaatctct
      661 acaggtgaaa tatacactgg ttggcgttta tgtcgcaaaa catgtcattc aggttcatct
      721 caccaataag gatatgagtg aagtggagga taagtgagtc tgctgtcaga gttttctggt
      781 gtatgtcagt aactgcagaa ccatgtctgc ttgatatggg agcttatgga ggctctctgt
      841 acaaggttag ggagctaaca aagcagggat aaaaagtccg cctgatgcag gcacggttcg
      901 ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat gttcaggcta tctttatctt
      961 gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca atagaagaac tgcaggcagt
     1021 actggtccca cattgtttgc tcagtcaact ggtgaagttc cgtagcgcgt aaattaatgc
     1081 tttgcatgaa acgttactgg agtggatgaa agaatacctg catatatcat tactgcgttg
     1141 gattaccagt acaacagatt gaatgaactg gacatgttga tagacggcat tgaaaagcag
     1201 cttataagca tagtgcggca gagttaattg gagagcactt cgccttctga gtattaagta
     1261 atcatggtgg tacctgcgga tactatctat tcactgtgct cggtcggcag ccttactgac
     1321 caaggagttg ggggagttga taacaaagtt gaaaaaacag ctctcaacca aggtggttat
     1381 tgtaacaata gcaaataagc tggtgtgacc tgtatcacaa taaccgatta tggacgtaaa
     1441 tacgacaaaa atatgtcagt atcaggcggt attaattgct gatgctgatg gacattaact
     1501 cttaataaaa gggtggatgc tgaaaggttg ttatggtgac ggacgtgatg acgacggaaa
     1561 agagtatgag gttttggatt taaagttcgt tcggggaata aggacccggt cagctaattc
     1621 tcatagtagt tcgcattgct ggatgcaata ctgtgctgca attactcgtc gatgtcggaa
     1681 caacggaagc cttgcaaacg gggatgtatt catacaggtg ttatgttata tggggtttta
     1741 ttgcaggggg ctataccaag agaatatatg atatctcatc agactgatgt gagggtaaat
     1801 gagaatagag taaacgaaca aggttgtttt ttggcgagga agcagatgta tgacaattct
     1861 tgtggagctg ccagtttact ctgtgctgca aaggaattag gggttgataa aataccacaa
     1921 tacaaagggt ctatgtccga aatgacaagg aagagttctc ttgatttgga taatcgttgt
     1981 gagcgggatt tatacttaat aactagtggt aattataatc cgagaataca caaagataat
     2041 atcgcggatg ccggttattc tatgcctgat aagatagtca tggctacgag gcttcttgga
     2101 ttgaatgctt atgtcgttga agaaagtaac attttttcgc aagtaattag ttttatttat
     2161 cccgatgcaa gggacttatt aattggcatg ggatgtaata tagtacatca acgagatgtg
     2221 ttatcttcta accaaagagt tttggaagct gttgctgtat cctttattgg agttcctgtc
     2281 ggcctccact gggtgttgtg tagacctgat ggaagctata tggatccagc tgtaggagaa
     2341 aattattctt gtttttcaac tatggaatta ggtgcaagaa gaagtaattc taactttata
     2401 ggatatacta aaataggtat ttccatagtt ataactaatg aggctttgta aattgtcgtt
     2461 ttttatctgt gaaggagctt gaatattttg gggcgggggg cataacaaat gatattactg
     2521 agtacaaatt tagctatatt gggcggatta gttgtgtggg aagacgaaat gtttctcttg
     2581 aaataagtga actccctcaa ggaatgattg tgtgcttaaa tatttaagtg ctggtaagtt
     2641 tgagaaggct gacatatttt gcagagcaaa cagtgattga cttggatgat atttttattt
     2701 tagtcactcg ttgtaattgg agtatgcttt attttatttg aaaatagatg tattttatac
     2761 ccgcaattta aattgtaagc cctatgaggg gtatggatat tataagttac tctttgacta
     2821 agcataactt ttggggttag aaaaataaat ctcttgttaa taattaatat gattttaaaa
     2881 tcaaacttgt ttataattat aaaataatgt aagtcggtca ttttatggga agattaggag
     2941 aataaaacag taattatatt tgagaaatct gctaatatat tcttaaagct tttttgatac
     3001 tgtgaacgaa gtctgttaat ggtggatttt ttgtttatca ccatgtaaaa aaattgagta
     3061 gtaaaagcgt tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa
     3121 tattttcggc cagtcactca tcttcacata aaattttgac catcatgccg catcccttag
     3181 ttgcacaatt cattttcaat gatatgaata ttactaattt caccagataa tgttctcgtg
     3241 gtcagcaggg tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat
     3301 aacagatagc cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat
     3361 tatctgaatt gggcagttta catcagtgtt cgagtttatg aagacgatag atggcgagaa
     3421 attatatctc gaatagcttc atctatttct ctctgaactg ttttgtttat tttagataat
     3481 gtaaatatta gagttctgtt tgttttattc tcttttcttt ttatgtaagt ccgtttgtct
     3541 ttagaaataa ataacgagtc aacttttagc gaagggtttt gctttctgtt tttgcttttc
     3601 tttatgatgt ttaaaatttc ttttttatat atgtccgggg gcaaatttgt atcaaggtct
     3661 tttatttctt cctttaatat tggtagtgta gaactaagag attcattagc cttttcaagt
     3721 cctttataat aattgaataa tatcttgtag tcattaaagt taagttctga cgcgattgga
     3781 aatagagata ttatttcctg tggaacgctt gctgcctgaa aggcacgagt gactttcgcg
     3841 cgagacagat tctctttttt ggctatgtct ttataggaca tccctgatac tttcagaaaa
     3901 ttaagaccaa taccaagttc tcggatgcta tgctcttttg ctgtttgtat atcgtttgct
     3961 agttttctgg catcaagagt agatatatat tcttttgaat atagaacttc aagatctgct
     4021 cctgcatata ttgcagatgc tcttctacga gtgccatcca gaatttcaat tctaccatca
     4081 atctcccttc ctattacagg gaagaattgt tgtagcttta tagtttttat gatatcggct
     4141 aatgattctt ctgttaggga ttcttgatcg cgtacattcg ttttttggtt gacgaaggtt
     4201 aaatcttgga tccttttatg aggaatcttg gctttgataa aggaaacttt attaccagaa
     4261 tggagtgtaa actctttctt ttggccttcc tttatactta acaagtcgtt gcacaaatcc
     4321 accatatcac accctgttta ttcatattga tttatattga caaaagttaa atgcagtgtg
     4381 agcaagattg tatgtaactg ttgatgatag tcaacggtag tcttaaaaat tactatttta
     4441 atagctggat tgatgcaatt tatctttatt ggataaaaga ataatattct tgtggagctc
     4501 tgatgtgaga gctccataga tgaaaaaatg cttatcaatt actatattga ttttgtacta
     4561 tttttgacta cagaaataag atctccaata gtaataatgt gttctaatgc agattcatct
     4621 atacgtagat tgaactcaaa actaatcata tccagcattt caatcagaga aagagaatcg
     4681 gcagaaagat cttcaataaa tttagtttct tcagatagtt tgctgtacgc tataccatag
     4741 caaaaggcca ctattgataa tattttttct tttatcatat tttaatcctt attgatattc
     4801 tttaatactt ttgataggga agtggttaca tcttttgctg ctttatatat attattgttt
     4861 tcagctgtaa tatcattgtt attttttaaa tcagatatat catctgtaag taattctgca
     4921 gaggtatcgt caatttttga caataccttg ctgagggcgc tagatacttc ttttgccttt
     4981 tcatagatcg catgattttt atcaattgta tctgcgttgg tttctgttac gtagtttatt
     5041 gtatttgcat ctgtaacttt taatttgttg tttattctgt gttctgttgt ggattttgta
     5101 atgtcctttg atttttcctg taactctcgt tcttgtgttc ctgatttagt tccaatgaga
     5161 gatataagat tacttaggga gtttgttact ttttttgaag cttcaaatat cgtttcccgt
     5221 gttaaatatg tgtctccatt tcttgataaa aaatcagtct gaagtacttt tgaatcagtg
     5281 tttgagttga atttattgat ttgcgaagct actctatgag aaagagaagc gctactttta
     5341 gttgtatctg atgagttatc attactgctt gtcactcctt ccgggtggag agggggggtg
     5401 gttgaagagt tctgtatgtt cagaggcctg tgtccccgag aaagagagtt atttgtagat
     5461 ctttcttgac tactcgtagt taacaggtct gaacctattc catccgaatt atctggagta
     5521 tggttgaaaa ccttttctaa agctgaatta tttgcaaatg tctggacagt atctgatata
     5581 gagtgtggta gaacggaagt tactttttga actagtgctc tttgtggatt tagtaatgag
     5641 tttccgttac gggataatag atcgtttgtc tgagaagtag gagactgtgc atcattttct
     5701 gggttctcgt gatagctatt gtcatatact ctgttgtcaa aaactctatt atcgtaagtt
     5761 ctattatcat tatttatatg atagtgaatt acaggttggg atgttttctc attggctccg
     5821 ggggttggtc cgccatctgg catatcttcg ggaggtgtag ggaggccatc ttggatagga
     5881 tgcgaatctt ttatatcttc taaaatatca ttaaggactt ttctcaacag ctcaggaaac
     5941 acggctactg tatttaagtc tcttgacagt ttacctatac caaatccttt actatcaata
     6001 gtatttttac gttgattgcg caaactatca aaggcaagtt ttgcctgtgc gtcaactaaa
     6061 tcatctagtt tgagagatga aacagtttca ttacttaact ttaattcagc aagaatatta
     6121 ttcttgataa atgatgatac ttcagtattg aggagttttt gtcccaatga taaagcttca
     6181 ccagatgtgt gttgttgtgc taatagtgca agtctgtgct caatgttttg tcgtaattga
     6241 tggagtttct tattctttgc cgaccatggt ccaaaggcag aatcttggga taatttatct
     6301 tttataatta gttccgaata ctgtgcgaca attttttgaa gtcccttttc gttttcaaaa
     6361 ttaagatcat tttgatgtac taaggagtac aaaacatttg aaaattcgag aatctcgctt
     6421 ttggcatacg cattcattga gttggaataa atgcttctta acacatcttc tttccctgag
     6481 gaagaaaata aaaaatcaag acatttctga ttaaaagacg ttgcaaagtt ttctttttca
     6541 gacactgatg ctggtgtttt tagagaagtt tgcgaactat ggatatcgga tattttgctt
     6601 tttaactcgc tgtattctgt tgatgatggt gaagttgcct tatataagaa tgttggcgct
     6661 tgagtattat ttacattatg catggcgcac ctcagaaatg gagaaaaagt ttatctgtat
     6721 ctgtacatga gcttattgta ctactcaaaa cctttactaa attatcaaaa atactattgg
     6781 cattactgta tttttgaact aaagtttgaa gattattttt cattgtttca tcttcggcag
     6841 agaatccggc attccatgcc tgatattttg cattatctag cacaacctcg ccatttccac
     6901 ctagattatc taagcttttt aacatattgt ctattggggt catgtttata ttgacaacat
     6961 atcccccgtt tttttcagat accttgccga ttgttccacc taattctgta agccatttat
     7021 ttgcttgttc cttactaaca gtattatttg ctggatatag cggtttatct ttatattttt
     7081 ccttgagctt tgtcaattcg tctttaagcg atttgacttg taatttcacg gagtttccgt
     7141 cgttacctcc gggagagatc cagccggcaa gactggaaag aacagcgcta aaatcttgat
     7201 acatttgagt atatgaacta acggcatgtt catatacttt cagatactgt tcattaatat
     7261 tattgattga ctttgcaatt ttatcccaca gttctctgtg agatatcatt tcatatccat
     7321 caagctcggc ttctttcggg gctgaatgta ataattctct tgcgtcttta ttaattggat
     7381 attctttctt ggaaagaata tccaatagtt gagcggattt gtttacatcc atgctaatct
     7441 gagatgaatg taatgctatt tcttctagcg atgttttagt caacgttttt tgtgagagat
     7501 ctttctttaa tgcctgattt gttgttctga tattatgaag ggtgtcgttg agcatagtaa
     7561 gggaacttac aggatgagaa ctggtcgttg tttttatatc agaattaact gtttcggttg
     7621 atgaaccgtt ggtattgttt gggctgaatg atgaggtgga aatactatta gtcagagttg
     7681 ttatattcat ggttatttcc ttataggaag agccatatat tattatggct cttggatata
     7741 ttaagctcga atgttaccag caatctgact ggctgtagaa ttccttgatt ggttgatgtt
     7801 gtcaattata ttcaataatt tttgtattaa ttgatttgtc gcttgggatg cttctttaga
     7861 tacttgggat gcttcctctg cttgtttact gctggcctga ctgattagtt gttcttcttc
     7921 ttcaagagca gatgtataac gccctcctga tgtggatata ttaccagcaa cggctgatgt
     7981 ctccattgtt gcacggccaa tgttttgttt ttgctgagca gaaagggtat tgagctcgta
     8041 agctcttctc tgggtgtcaa ttttatcctg caaagaaata tcgggagaac taagagaagt
     8101 tttatgttca gttgacaggg atatattatc tggtgccagt ttatttttac ctaaaatttt
     8161 tgtgctagag ttagtttgtg gtgaggtgat atttgtatct atttgtttat ttaaccctaa
     8221 tttagaacct gcaagctctt tttcaagaga ttgagcagtg gcaaggttct ttcttaaggc
     8281 tcctttttgt tcgctaatcc ctgaatgcgt ttttttggca ccgatacccg ttatacctac
     8341 ttgtgtgacc gctccagtaa tgcttgatga tagggctgct aggccttgcc gaacaatgtt
     8401 ctctgcagct gattttgtag catcgaacgc aatcaatgac aattgagagc ccaatctagt
     8461 ttctgcagtg cggagagcag aaagtagaac ggctacacta ataattaaag aaacagcatt
     8521 agaagaaaga ctggaaatat ccagggtgtt ctctggggta attgtgtgac tcatttctga
     8581 aagatctttc atctttttgt cagtaaggcg agcaatgatt tctgaatcat gctgaacctg
     8641 ctctgataac tttaaaagtt gatcatcatt taatgtagtt gttaatacgg gatttttacc
     8701 gacattaagt ggaatatgtg ctgctaactg ctgataattt tgtgattttt gtgtttcgga
     8761 agaactttga gtttgttttg tagatatatc tgtatataaa atcggggctg attttgtgtt
     8821 ttgaatttcc ataacattct ccttatttgt atcaagcagt agtttgttgc aaaattgctt
     8881 ttgcaacatc agttctatta gcctgagagt tggacattga ggctaacagc tctgcaatta
     8941 cttcctgcaa ttggccgaat ttttctattg cgtcaccgat gtattttgac agttgttcaa
     9001 cttgatattt cgataatgtc aggtctgcta gatttttcga cgcgttgttt tggaaaacag
     9061 cagaagcgac atttcctccc gcttgtgtgg cagagttaac actttctcct aataaaactg
     9121 cttggtttaa atgggtggaa ataatttgtt tggatattac ttcatcaccc gctgcaccaa
     9181 gaaatttatt taatctggca acagcattag tgattaaatc gtccagttga gaagaaaaat
     9241 tcttgagaaa ctttggtata aggtctgtga gggttttacc tattattttg ccaatattct
     9301 ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac gagaacgact gctgcaacca
     9361 agacaagagc gcctgcgatt gcccccagaa tagagccaat cattttggct tttttcgagt
     9421 cgacgcccaa gccttcgagc atttttgtaa atgcatctga aaggagtttg attaagggtt
     9481 caatgactgc tttcatgatc ggattcaggg cttgttccat gaaggaattg ccggtcgctg
     9541 cttgtactat agcatccgta accataagag ctaaaccaac agctgccagt gctagagatg
     9601 ctcctccaga aaaagctgct gcaacaacac taacgatagt taaaagtgcc ccaagtattt
     9661 tcccaacaca acccattact ctgttgagtt cttctgcttt acgtacttca gcagcatact
     9721 catcagattt tctctccatt tcagtttttc ttgattcttg gagagactgg aatagagcca
     9781 gatcattttt taaagattct tcattatttt ttccaactag ttgaataaag gttgccatca
     9841 attgagtaac actggcaagt ccggttaatg atttctgctg ggttgatagc tgttcagcag
     9901 atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt ttcgagttgc attgatttat
     9961 ctgtaagttt gctatgaatt gacagagttt tctgctcaat caatgtcctg tctttaactg
    10021 ctgcgtcttt tttgatagtg agttgtattt cttcccgtct taatttttta ttttctggtg
    10081 aatctggggc gagttcggat aatcttgttt gaatttggtt aattttattt tctaggtctt
    10141 ttattttaga atctgcgttt tttagtttat taatttgttt ttcatagtct ctggttagtc
    10201 cttcagtttc agatagaaga gtattaattt tatcggagaa ttctaggttt ttttgctgtc
    10261 ttgcctgttg ttgggacttc caggcagtaa ttttatttgt taatgcagtt aaagattttt
    10321 caccaagtat ttgaataagg tttccaatta aaagcgttag ttgggaactt gcatttaatg
    10381 actttggtgc tttaagttca gggattaata tattcgaagt tgagtgtgca ttagttgtat
    10441 taatattttt gttagcagca agatcagcaa ttgtaagaga aaataatttg ttagctgcat
    10501 catttgcagc ctggatagta ttgtctccaa gctcagtgga agccaatatt ttggcaagag
    10561 gcaaaccagt ggttgtggtg cttacattat gcataataat tactccttga tatcctgaat
    10621 tgcgtctaag tatgattgtg cttttatttt taatttttca tcattgctgt gttgaattac
    10681 gagttcgaag cactctttgg ctttcaaggg ggctttcaac cgaagctggc attgtccagt
    10741 atggaatacc ggtgtatagt cattttttcc taatgcaaaa gcgacagcat aaaggtctgc
    10801 tgcttgttgg aactgttctt ttatctgata aatagctgcg agtcccataa tgtagtctac
    10861 attgtaaaag tcgtatatac ataaaaatct gaagaaaact tcagcttcct ctattcttcc
    10921 tttgttgtaa aagtcataag catatgaata aatgtcatcc atcatatcat caggaattgc
    10981 attaatatct ttcagtgtag cgccagagtt aattgcatca attactgcag tagagatgct
    11041 ttcattttca gtgatattta aagacataag gtctcctttt tgtctgcatt actttaataa
    11101 gtataagatt taatataaac agatttaatt tgtattgctt tgacggtata cagcctttag
    11161 aacctctgcg cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta
    11221 gacggaactg gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc
    11281 actgctataa cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc
    11341 tgtatgactg atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt
    11401 ttcaatagct gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat
    11461 ggccatttca tctatacttc tgtttatcat tgaagagaca taagaggttc gctcttggct
    11521 catccaacaa aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc
    11581 cctgatttta tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa
    11641 gttaaaaaca gataatattt tctttacaag cttattgtga cttaacgaat caaatgatgg
    11701 gcgaggaatc gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc
    11761 tatatgttag ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa
    11821 ttcgtacaaa gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt
    11881 caaaacacat tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa
    11941 agagtagtta aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg
    12001 ttcattaaga agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat
    12061 ttgttcttcg agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt
    12121 tatttcataa agtttatcat atagtttgcg acacatttct atggccttct atatattaga
    12181 atgagagtta ttcaataaaa gcttattgac ctgtaataag tcgtgaatgt aaagtttggt
    12241 tccaagatct ggcgatttaa gagaattgta ataatcaata gtttttattg acttttcgat
    12301 aattacttct tttttatctg ttgctccggt ttttaattcg tttaatacac gagacatatt
    12361 gctgggtgct gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc
    12421 tgaagataca ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt
    12481 tggggtgtgg ataaggttaa ttttattgaa acttttaaga tagctacgtc gtaattgagt
    12541 caatctggaa agatcaaaat cagattcaat atacttcatt atctcgttat aagactgatt
    12601 gagactttca atgcgttgcc taactttatt tgcatacgcg tatacattgt taggtgtagc
    12661 aaccaattta acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt
    12721 aagtgccata ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct
    12781 gaaagcatct gctttctttt ggcatataaa ttttgacatg tttgtttcat caaggccaaa
    12841 aaggttatag ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac
    12901 tttatcagca ctgacccccc aataaccata gggatctttt ttttgattag ctctgggaaa
    12961 aaatattttt ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg
    13021 gcctgcatca agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgtttata
    13081 ggtatcgaca ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt
    13141 aacattcccg ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt
    13201 ttttatactg atactggtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa
    13261 cactaaatgt ctttcaagat ttttggctgt accgatgcca tgaaaactct ctgcggtatt
    13321 caacattaat ttgatgtctt tacctgtaaa agaatgttga attgaatttt tgactgcttc
    13381 ataggcggca aacgcacttt ctgcagattt ttcgctagct aatgtcttga ggaaaagcaa
    13441 taaggatttt tgattcgcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt
    13501 tatctttgca ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg
    13561 tgctaccaat ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct
    13621 tgcgtcaatg aaattgctaa ttttgagaat catactttat taactctcca ttacgtggtg
    13681 attgtgggga tgtaaaatgt atttgatata taatatattc aattaataaa tttagaaact
    13741 tgagcctgtt aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat
    13801 atgtagtgct ggtttcatca tgaaatccca caagttaaag tgtctgatat atcaggctcg
    13861 gagtgttata gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag
    13921 gtacaaaact gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa
    13981 tttgggattg catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac
    14041 cggaaaccaa aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagagagaaa
    14101 taagggggtc gaagcgctta atcgattata tttacagaac caaacaagct taacagggaa
    14161 aggccttttg tttgcgcgtg acagagctgc agttttttac gaggcaataa agttggcagg
    14221 tggtgatacg tcaaaaatta aagccatgat ggaacaatta gatacctata aacttggtga
    14281 agttgataaa agaaatatta atgagcttaa taaagtaata agtgaagaga taagagcaca
    14341 gctaggcatt aaaaataaaa aggaattgca atcggaaata aaacaaatat ttactaatta
    14401 tttaaataat aagaattggg aaccgatcaa caaaaatatt aattataatg ggaaaaatta
    14461 tggttttcaa ttaacacccg cctctcatat gaagataggg aataaaaata tatttgtcaa
    14521 agagtataat ggaaaaggaa tttgttgtgc ttctacgaga gagagtaagc atattgcgaa
    14581 tatgtggctt tcaaaagtgg tggatgacga ggggaaggaa attttttcgg gtatcagaca
    14641 tggagttata tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgcagctcg
    14701 taataaagct gaggagttag caagcgcagc attatatagc agacaagaat tattatcaca
    14761 ggctttgtct ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac
    14821 cagtttaact gggggagagg agagtatgtt aaaagatcag gtaaaggcat taaaagagtt
    14881 aaactctaaa aggggggaac caacgaaatt attaattcgg aatagtgatg gtcttctgca
    14941 agaagtaaag gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct
    15001 aaaaatgggc ttaggctgga ggaatgttga caagcttaat ggtgaatcaa tatgttcttt
    15061 gttgggggat aatttcctca aaaatggtgt gattggaggt tgggctgcag aagctataga
    15121 aaaaaatcca ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattct
    15181 aaacaagaag ttacaaaaga atgataatgg agagccgtat aaattgtcac aaagaatggc
    15241 tctattggct tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag
    15301 aacaggtatg caggatgctg aaattaaaag agaaataata agaaaacatg aaacagggca
    15361 gttttctcaa ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct
    15421 aatgaatagt ggtaatatgg aaatccaaga gatgaatact ggtgtgcctg gaaataaagt
    15481 tatgaaaaaa ttaccgctat cttcgttaga gctgtcttat gctgaaagaa taggggaccc
    15541 caaaatatgg aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga
    15601 tttagcagat gttatttgcc gagccttggg tatcccttca attgataccg atgatcaagc
    15661 aatcatgctt gatgatgatg tgttaatata tattgaaaaa gaaggagatt caataaattt
    15721 attatgccct ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct
    15781 aaattactca gaaaagatat gtttagctac tgatgatgaa ggtggaagtc taattgcgcg
    15841 attagatctt accgggataa atgaattcga ggatatatat gttaatacag aatattatat
    15901 ttcgcgtgtg cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc
    15961 cgtttcgtat ttatttttct gtgttttatc ccatatttag ggagagctga ttgttgggat
    16021 aaggctggtg aaaggtacaa cattccatcg agtttgttaa aagcgattgc ggaaaaagaa
    16081 tccggattta ataaatttgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata
    16141 atgcaaataa atgactttca ttctaaaaga cttagagaaa tgggatatac tgaggaaatg
    16201 ctaattagtc atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg
    16261 ctgatgtatg gaagagggtg ggaggctgtt ggtgcctata atgcaggaac atctccgaaa
    16321 aagaaaaaag aaagattaaa gtacgcagag gatatctata aacgctattt gcggatagca
    16381 gcagagagca agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact
    16441 caaatcttgc accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc
    16501 cgttatatta tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg
    16561 gaaacgataa tttcccagaa aatattattc cagtaacaga tagtaaatcg gatggtataa
    16621 tttatttggc cataagtaaa gataatatct gtcaatttag tgatgaaaaa ggtgaacaaa
    16681 ttgatatcaa ttcacaattt aactcatttg aatatgacgg agtttctttt caacttaaaa
    16741 atatgatgga ggataaatcg agagttcata tactaaacgg aatgtataat aaaccctcgg
    16801 tgtttttttt ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga
    16861 aagatgaagt aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca
    16921 caggacagtg caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcatctgtag
    16981 ccttaaataa aagcggattt accaaatgca gatatattct ggtgtccaac aaagaaataa
    17041 atagaattca acaatatatc aatcaacgat tcccatatat caatttgtat gtgttgaatt
    17101 tagtgagtga taaagcggaa ttactggtat ttttaagtaa agaaagaaat agttcaaaag
    17161 ataccgaact tgagaaattg aaaaatgctc tgatagttga atttccgtat atcaaaaata
    17221 ttaaattcaa ctatcttagc gatcataatg caagggagga tgctaaaggt attttcacaa
    17281 aggtaaatgt ccagtataaa gagatttgtg aaactaataa agttacatat tctgtcagag
    17341 aagaactgac agatgaaaag cttgagctca ttaatagact gatttctgat cataaaaatg
    17401 tatatggaga tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta
    17461 aatcatatct taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt
    17521 tagataaaaa taagtgagga taaaatgagt gttacagtac cggataaaga ttggactctg
    17581 agttcattat ctgaaacttt tgatgatgga actcaaacat tacaagggca actaacatcg
    17641 gcactaaatg cattagctga aaatccttcg aatccacagt tgctggctga ataccaaagt
    17701 aaattatctg aatatacatt atataggaac gcgcaatcta atactgtgaa agtgattaag
    17761 gatgttgatg ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt
    17821 tatccagtca atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca
    17881 agcctggaag acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa
    17941 gtatcacaaa taatggagat ggtaagccat ccagaatcat taaacccaga atctttggcc
    18001 aagttacaga cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca
    18061 agaaaaacag tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata
    18121 aatgttttat tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa
    18181 tttctaattt atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata
    18241 ttactgctag aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga
    18301 catttgcatc ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg
    18361 atatctcaca aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc
    18421 gtttatatag tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta
    18481 tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga
    18541 tgcatatatc agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta
    18601 atattaagcg atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca
    18661 tattaactcc gaaagaagaa tatgtttata caaatgtaca acctgttaag gaaattaaat
    18721 cggaattttt aacaaatgaa gtaatatatg tatttctcgg gatggctgta ctagttgtca
    18781 ttcttttggt atgggcattc aaaacagggg ggttcaagag aaacaaaata tgataagaat
    18841 ggatggaatt tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag
    18901 aaatcggttg aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa
    18961 taatctcata attaacaaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac
    19021 cgctatgttt gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca
    19081 agagtctcgg ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag
    19141 taagcaaggg gaggccgcta ttcgaagtgg tttttggcat cttattgagt ggaaggaaaa
    19201 tatatctgtt ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg
    19261 aaattatacg cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa
    19321 aaggcacttt accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg
    19381 aaaaggaatt atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt
    19441 gtgttataaa ggatgctacg aaaaaagctg aatcactgcg tatagatgct gtatgcgatg
    19501 gttatcaaat tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga
    19561 agttaaagca aaatgaaaac agaagaaata ttgaggacta tataactagt ttgctttcag
    19621 aaaatttaca tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc
    19681 gtaatacagt aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa
    19741 agttagaact tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg
    19801 aagggaataa ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg
    19861 taatatcagg tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt
    19921 tcaaagagtt agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc
    19981 ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa
    20041 gaatttgtta atttaaatga gagattatca tcttcgcaat catttgatga gaatataatc
    20101 tatgaaataa tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg
    20161 cacaataaga ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa
    20221 agttcacttc ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt
    20281 tatgcatcat tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat
    20341 tcattaatta gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa
    20401 gttgaaaata tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc
    20461 atcatctaat tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat
    20521 tccaaatgat ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa
    20581 atgtattagt ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa
    20641 tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt
    20701 ttctatgctg aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat
    20761 agagaaggat ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta
    20821 aatataaagg tctaaataca agtaatatgt tttacattta ctctagtgga cataaaccag
    20881 ttaacgttga gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga
    20941 agggtttttt tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta
    21001 tcgctttaac attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc
    21061 tctagtagag catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg
    21121 ggatagttgt aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc
    21181 tgaccagaat gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt
    21241 tctttatctt ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga
    21301 gcattatggt gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa
    21361 agtcaaagaa caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca
    21421 caatcaaaca atcacttctg ctgccatgaa taatgggtat gcatcaacat cccatttttc
    21481 aaatgagata aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt
    21541 agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt
    21601 taccattgat agttaatgct aataatattg actctcattt attagaacaa aatgacatag
    21661 cgaaatatgt tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat
    21721 taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg
    21781 atttatctaa tccagaagaa atgcttgaaa aaatgacatt acttgtggga ctcatatggt
    21841 ataaagatgg aaatgcatta tacatatatg actctggaga attaataagc aaagttattt
    21901 tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg
    21961 atcacagata tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc
    22021 cggcattggt tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta
    22081 ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca
    22141 gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac
    22201 gtttattaaa taatggtaag gctctgagta acagacaagc caaaaatgat ccgatgccac
    22261 cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta
    22321 ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat
    22381 tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact cagttagatg
    22441 tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa
    22501 ataaccttgg cgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcatcat
    22561 ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag
    22621 ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac
    22681 aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg
    22741 agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct
    22801 tttcatctca gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc
    22861 agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca
    22921 aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc
    22981 atgaaacgaa ctctaacgaa atagtatcta ttccttttct ctcctctatt cctgtaatcg
    23041 gtaatgtgtt caagtataaa acaaggaata tcagtaatat tgtaagggtt tttctgattc
    23101 aaccccgtga gataaaggag tcaagttatt ataatactgc agaatataag agcctcatat
    23161 ctgaaagaga aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag
    23221 acgaaaaatc attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg
    23281 atgttaaaaa tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa
    23341 attcagatga tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca
    23401 agtatattga ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag
    23461 accttgagaa actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag
    23521 aagatgaaat taatcacaag atttttgatt taaagagaac tttaaaagat aaccttcctc
    23581 tagatcagga ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct
    23641 tagcattaag ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaatcattaa
    23701 ctaaaattat taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt
    23761 cagctataca ggctaaatta tttggcaaca aaatgaaact tgaaccgcag cttttgcgtg
    23821 catgttatcg tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc
    23881 ttggtaattt tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga
    23941 ttgtagacat ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat
    24001 ctaaattaat tgcaattaag atgattagaa cttcagatgt aatttttatg aagaaactgg
    24061 aatcctcaag cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat
    24121 tatatatttt tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagacgtat
    24181 cacgagccag tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa
    24241 gtcctcatga tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag
    24301 agcttgtcac aagtgcatac aagaaagagc tttctagata acaggagatg aaagtgatcc
    24361 agtcttttct taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta
    24421 tgatcattgc tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc
    24481 ttaatattgt acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga
    24541 gtttttctac attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa
    24601 taagtactag tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg
    24661 gccagtttgt cattggtgat agtctggctg taggttttgt tattttttca atagtaactg
    24721 ttgtgcagtt cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct
    24781 tttctcttga cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa
    24841 ttattgatgc cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt
    24901 atggttcatt tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta
    24961 ttatttttgt caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtctc
    25021 tatctggtgc attatctact tataccatac taactattgg tgacggattg gtctctcaga
    25081 ttcctgctct gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata
    25141 gcgataatat gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg
    25201 ttacatcagc tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt
    25261 tcctgatagc agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa
    25321 aaagtctgtc tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc
    25381 atgactcatc tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt
    25441 taattttatt atttgccgaa aataagataa gtgcaaatga tatggaaggt cttattgaaa
    25501 ggataagaag tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa
    25561 caagcaatga gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata
    25621 gttttaatat atactttgac aaagtgtgta ttacagatga aaatggagat atagatgctc
    25681 taggtattcc tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt
    25741 catatacaga aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt
    25801 atcaccagtt gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa
    25861 caaaaaatat gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc
    25921 gacatgtgac tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt
    25981 ctgttcgcaa tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag
    26041 atgtcataac attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa
    26101 tagctgtttc tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa
    26161 gaaaagggat aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg
    26221 atgaggtaat ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt
    26281 tcgttctttt ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat
    26341 ttaagagtat actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat
    26401 taaagactat ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag
    26461 atagtctttt cacgattggt tgtcctccat caattattac agatttagac agccattcag
    26521 cgattaccat ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg
    26581 ttatgttatg ggctaatttc gatgcaccaa gtgatgtaaa acttcagtca tctgcttata
    26641 acatattaaa tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc
    26701 atcgttctga tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg
    26761 ggattgtttt tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgagg
    26821 tcttataatc aatgagctat ataaaattgc tcactcaatt atcttttcct aatagaatct
    26881 cggggccaat cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc
    26941 aggctggaat tgaaagtaat gaaattattg caagagctca ggttgtagga tttcatgatg
    27001 aaaaaacaat attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta
    27061 agcccactgc ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca
    27121 atcctttagg ggaggttact gataagtttg ctgttacgga taacagtgaa attctttatc
    27181 gacctgtaga taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt
    27241 taacaggtgt taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga
    27301 tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg
    27361 gtgctgatat atatgttatt gggttaattg gtgagcgagg tcgagaggtt acagaaacgg
    27421 ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg
    27481 attactcttc ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt
    27541 ttaggactga aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag
    27601 cattacgtga tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt
    27661 cggtttttga tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct
    27721 ctattactgc attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg
    27781 aagaggtaag atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag
    27841 gacaatttcc tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg
    27901 atgaaaaaca tcgtattatg gccactgcat ttcgggagct actttctgaa atagaagagc
    27961 taaggacaat tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa
    28021 tctacaacaa aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta
    28081 cttatgagca gacaatggag cttcttggtg aaacaattag ataaggtttt aaaaatcaaa
    28141 gataaatatc aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa
    28201 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
    28261 gaatgacttg gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
    28321 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
    28381 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
    28441 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
    28501 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
    28561 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
    28621 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
    28681 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
    28741 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
    28801 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa
    28861 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
    28921 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
    28981 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
    29041 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
    29101 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
    29161 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
    29221 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
    29281 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca
    29341 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
    29401 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
    29461 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
    29521 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
    29581 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
    29641 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
    29701 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
    29761 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
    29821 taatcaagtt tctcttgact tattgcatca tcttttagag ttttgcttag gttcatctaa
    29881 tgtaaggctg gctactttaa aaagaattcg tactggtgat ataatcatag ttcagaaact
    29941 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
    30001 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gatcacacag aagtttcttt
    30061 ggcattattc aattatgatg atatcaatgt aaaagtggac tttatccttt tagaaaaaaa
    30121 gatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
    30181 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
    30241 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
    30301 tgctgagtga tatgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
    30361 ttgctgctgg tacttgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
    30421 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
    30481 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggcccgcaaa
    30541 aatttgatac aatatcagat attgtccgtt tttctgattc tggcttgatt gaatacaagc
    30601 aatacttgaa aaagcataca gatcttgagt tggcacgttt tttccaaagg tctgaagaag
    30661 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
    30721 catatgcgct aagtgaaatt aaagatgcgt tcaaaatagg tttttattta tatttgccat
    30781 ttattgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
    30841 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
    30901 ggatcctgtc taaggccttg attgaacaat atatcaatgt tcctgcttag atgatggagg
    30961 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
    31021 cttatggcca gtaggtatag ctacggttat tggattaact attggtttat tacagacagt
    31081 gactcaactt caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
    31141 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
    31201 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
    31261 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
    31321 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
    31381 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
    31441 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
    31501 ttttggatat ttcatgctgt tggtagtatt attgacaacc agcgtggggc aacgcttagt
    31561 agttcaattg atcctgccaa tggtgttgat acgtctgaat tggcaaaatt tttcaatctt
    31621 ttttctgcag ttgtatttct atacagtggt ggaatggtct ttattttaga atccatacaa
    31681 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
    31741 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
    31801 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
    31861 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
    31921 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
    31981 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
    32041 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac agttgttatt
    32101 attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta
    32161 tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg
    32221 gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg
    32281 ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt
    32341 tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa
    32401 tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt
    32461 aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt
    32521 tggggggggc tatttaagga tataatatta tttttcttag cattttctat ttttgttatt
    32581 attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag
    32641 gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt
    32701 gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta
    32761 gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct
    32821 gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca
    32881 aatgaagttg gtataccaac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca
    32941 catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt
    33001 tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt
    33061 aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt
    33121 aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaacaa ggatataata
    33181 aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt
    33241 tgtgctcttg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact
    33301 cttttgttga tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag
    33361 aaaattgtta tgtttttgtt aggaactgaa ctaaataata tatgataaga cagcaaaaac
    33421 gactgaccat aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg
    33481 ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac
    33541 taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta
    33601 aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac
    33661 caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt
    33721 tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg
    33781 gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag
    33841 gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct
    33901 cttaaaacga tagtggctca ggctttcatt tttcagcgtt ccccagaaga tttccatcgg
    33961 agcgttgtcg taacagttac ctttacgcga cattgatgtt ttcagaccag actgctcctg
    34021 tatgacccgg taatcgtatg cgcagtactg tgaacctcga tcggtgtggt ggattagccc
    34081 ggcaggtggg cgctggctcc tgagcgccat aaacagggct ttacccgtca attctttcgt
    34141 catgc
//
LOCUS       DAJVTY010000024        33936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVTY010000024 DAJVTY010000000
VERSION     DAJVTY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33936
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.024"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(270..482)
                     /gene="rpmE"
                     /locus_tag="OQQ66_001059"
     CDS             complement(270..482)
                     /gene="rpmE"
                     /locus_tag="OQQ66_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1904066.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            685..2883
                     /gene="priA"
                     /locus_tag="OQQ66_001060"
     CDS             685..2883
                     /gene="priA"
                     /locus_tag="OQQ66_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1904067.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNACPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3039..4064
                     /gene="cytR"
                     /locus_tag="OQQ66_001061"
     CDS             3039..4064
                     /gene="cytR"
                     /locus_tag="OQQ66_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1904068.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4156..5115
                     /gene="ftsN"
                     /locus_tag="OQQ66_001062"
     CDS             4156..5115
                     /gene="ftsN"
                     /locus_tag="OQQ66_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1904069.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5208..5738
                     /gene="hslV"
                     /locus_tag="OQQ66_001063"
     CDS             5208..5738
                     /gene="hslV"
                     /locus_tag="OQQ66_001063"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1904070.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5748..7079
                     /gene="hslU"
                     /locus_tag="OQQ66_001064"
     CDS             5748..7079
                     /gene="hslU"
                     /locus_tag="OQQ66_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1904071.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7146..8072
                     /gene="menA"
                     /locus_tag="OQQ66_001065"
     CDS             7146..8072
                     /gene="menA"
                     /locus_tag="OQQ66_001065"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1904072.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8165..8650
                     /gene="rraA"
                     /locus_tag="OQQ66_001066"
     CDS             8165..8650
                     /gene="rraA"
                     /locus_tag="OQQ66_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1904073.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8735..8980)
                     /gene="zapB"
                     /locus_tag="OQQ66_001067"
     CDS             complement(8735..8980)
                     /gene="zapB"
                     /locus_tag="OQQ66_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1904074.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9406..10251
                     /gene="glpF"
                     /locus_tag="OQQ66_001068"
     CDS             9406..10251
                     /gene="glpF"
                     /locus_tag="OQQ66_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS1904075.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10274..11782
                     /gene="glpK"
                     /locus_tag="OQQ66_001069"
     CDS             10274..11782
                     /gene="glpK"
                     /locus_tag="OQQ66_001069"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1904076.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11917..12927
                     /gene="glpX"
                     /locus_tag="OQQ66_001070"
     CDS             11917..12927
                     /gene="glpX"
                     /locus_tag="OQQ66_001070"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1904077.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAVLGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13024..13770
                     /gene="fpr"
                     /locus_tag="OQQ66_001071"
     CDS             13024..13770
                     /gene="fpr"
                     /locus_tag="OQQ66_001071"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1904078.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13775..14203)
                     /gene="uspD"
                     /locus_tag="OQQ66_001072"
     CDS             complement(13775..14203)
                     /gene="uspD"
                     /locus_tag="OQQ66_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1904079.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14230..14529)
                     /locus_tag="OQQ66_001073"
     CDS             complement(14230..14529)
                     /locus_tag="OQQ66_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1904080.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14741..15181)
                     /locus_tag="OQQ66_001074"
     CDS             complement(14741..15181)
                     /locus_tag="OQQ66_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1904081.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15282..15881
                     /locus_tag="OQQ66_001075"
     CDS             15282..15881
                     /locus_tag="OQQ66_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1904082.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15989..16756
                     /gene="tpiA"
                     /locus_tag="OQQ66_001076"
     CDS             15989..16756
                     /gene="tpiA"
                     /locus_tag="OQQ66_001076"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1904083.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16811..17566)
                     /gene="cdh"
                     /locus_tag="OQQ66_001077"
     CDS             complement(16811..17566)
                     /gene="cdh"
                     /locus_tag="OQQ66_001077"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1904084.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17673..18662)
                     /gene="sbp"
                     /locus_tag="OQQ66_001078"
     CDS             complement(17673..18662)
                     /gene="sbp"
                     /locus_tag="OQQ66_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1904085.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18982..19944)
                     /gene="pfkA"
                     /locus_tag="OQQ66_001079"
     CDS             complement(18982..19944)
                     /gene="pfkA"
                     /locus_tag="OQQ66_001079"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1904086.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGSMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20125..21027)
                     /gene="fieF"
                     /locus_tag="OQQ66_001080"
     CDS             complement(20125..21027)
                     /gene="fieF"
                     /locus_tag="OQQ66_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1904087.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21175..21687)
                     /gene="cpxP"
                     /locus_tag="OQQ66_001081"
     CDS             complement(21175..21687)
                     /gene="cpxP"
                     /locus_tag="OQQ66_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1904088.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21837..22535
                     /gene="cpxR"
                     /locus_tag="OQQ66_001082"
     CDS             21837..22535
                     /gene="cpxR"
                     /locus_tag="OQQ66_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1904089.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22532..23905
                     /gene="cpxA"
                     /locus_tag="OQQ66_001083"
     CDS             22532..23905
                     /gene="cpxA"
                     /locus_tag="OQQ66_001083"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1904090.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24011..24685)
                     /gene="yiiM"
                     /locus_tag="OQQ66_001084"
     CDS             complement(24011..24685)
                     /gene="yiiM"
                     /locus_tag="OQQ66_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1904091.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24834..25799)
                     /gene="kdgT"
                     /locus_tag="OQQ66_001085"
     CDS             complement(24834..25799)
                     /gene="kdgT"
                     /locus_tag="OQQ66_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1904092.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26078..26698)
                     /gene="sodA"
                     /locus_tag="OQQ66_001086"
     CDS             complement(26078..26698)
                     /gene="sodA"
                     /locus_tag="OQQ66_001086"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1904093.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26982..28016
                     /gene="rhaT"
                     /locus_tag="OQQ66_001087"
     CDS             26982..28016
                     /gene="rhaT"
                     /locus_tag="OQQ66_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS1904094.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28013..28879)
                     /gene="rhaR"
                     /locus_tag="OQQ66_001088"
     CDS             complement(28013..28879)
                     /gene="rhaR"
                     /locus_tag="OQQ66_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1904095.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28935..29771)
                     /gene="rhaS"
                     /locus_tag="OQQ66_001089"
     CDS             complement(28935..29771)
                     /gene="rhaS"
                     /locus_tag="OQQ66_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1904096.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30059..31471
                     /gene="rhaB"
                     /locus_tag="OQQ66_001090"
     CDS             30059..31471
                     /gene="rhaB"
                     /locus_tag="OQQ66_001090"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1904097.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31468..32727
                     /gene="rhaA"
                     /locus_tag="OQQ66_001091"
     CDS             31468..32727
                     /gene="rhaA"
                     /locus_tag="OQQ66_001091"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1904098.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNFHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32810..33634
                     /gene="rhaD"
                     /locus_tag="OQQ66_001092"
     CDS             32810..33634
                     /gene="rhaD"
                     /locus_tag="OQQ66_001092"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS1904099.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33644..>33936
                     /gene="rhaM"
                     /locus_tag="OQQ66_001093"
     CDS             33644..>33936
                     /gene="rhaM"
                     /locus_tag="OQQ66_001093"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1904100.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8325 a         8849 c         8795 g         7967 t
ORIGIN      
        1 gaacaggcca cgccggtggg agcaccaatg cgcaccccgg ctgaaccctg aacaatgcta
       61 ccgccatact gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat cgtaaaatcc
      121 ttttgaaata aataagaacc agaagcctag cgccagtcaa caacaggaaa tgataatgag
      181 ttaacacccc ggaatttttc agaatttata aaaggcaaaa ttcaggcaca aaaaaagcgc
      241 cgtgcggcgc ttttttcgga aatccggtct tatttgctgc ccgggatgtt gaaacgcttg
      301 ttgaagcggt caacacggcc accggtagca acatcacgct gtttgccagt gaagaacggg
      361 tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa cagtggagcg gattttcatt
      421 acgttaccgc aagagcagct agcagtaatt tcttcgtatt tcgggtgaat atcttttttc
      481 atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc
      541 gatgttaaaa gtatagcttc aatacgatca tttcgtacga aggcgcgaaa tcatacagaa
      601 attaaccagc gtatgcaaac tgatccgcac tcttctacgg caatgtgtat actaacccac
      661 cgaatttcaa gtcaggatga tgctatgccc gttgcccacg ttgccttgcc cgttccgctt
      721 cctcgtacct ttgactatct gctgccagaa ggcatgacgg ttaaagccgg gtgtcgcgtg
      781 cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg tggtgtcagt tagcgatgtc
      841 agtgagctgc cgcttaacga gctgaaagcg gtagtggaag tgctggacgt tgagccggta
      901 tttactcact ccgtctggcg gttactgctc tgggcggcag attactatca tcatccgatt
      961 ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc tgcggcgaac
     1021 gcgccgatgt ggtactggtt tgccactgaa caaggccagg cggtggatct gaacagcctg
     1081 aaacgctccc ccaagcaaca acaggcgctg gcggcgttac ggcaaggcaa aatctggcgc
     1141 gaccaggtcg ccacgctcga atttaatgat gccgcgttgc aggcgctacg caaaaaaggt
     1201 ctgtgtgatt tagcaagtga aacaccagag tttagcgact ggcgaacgaa ctatgccgtt
     1261 tctggtgagc ggttgcgatt gaataccgaa caggccaccg ccgttggcgc aattcatagc
     1321 gcggcagata ctttttctgc ctggctgctg gcgggcgtta ccggttccgg taaaacggag
     1381 gtttatctca gtgtactgga aaacgtgctc gctcagggca aacaggcgct ggtgatggtg
     1441 ccggaaatcg gcctgacacc gcaaactatc gcccgttttc gtgaacgttt taatgccccc
     1501 gtggaagttc tgcattccgg cctgaacgac agcgagcggc tttctgcgtg gttgaaagcg
     1561 aaaaatggcg aggcggcgat tgtgatcggc acccgctccg cgctgtttac gccgtttaaa
     1621 aatctcggcg tgattgtcat tgatgaagag cacgacagct cctacaagca gcaggaaggc
     1681 tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc acagcgagca aatcccgatt
     1741 attcttggct ccgcaacgcc cgcgctggaa acgttatgca acgtccagca gaaaaaatac
     1801 cgcctgctgc gcctgacccg tcgggcaggg aatgcttgtc cggcaattca acatgtgctg
     1861 gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc
     1921 cagcatttac aggccaacaa tcaggttatc ctctttctta atcgtcgggg ttttgcgcct
     1981 gcgctgctgt gccacgactg cggctggata gccgaatgcc cacgttgcga tcactactac
     2041 acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg
     2101 ccgcgccagt gcccttcctg cggttccacg cacctggtcc ccgtggggct gggcaccgaa
     2161 cagcttgaac agacgcttgc gccgatgttc cccggcgtgc ccatttcacg tatcgaccgc
     2221 gataccacca gccgcaaagg ggcactggaa cagcaactgg cggaagtgca tcgcggcggc
     2281 gcgaggattt tgatcggcac acaaatgctg gcgaaaggtc accactttcc ggatgtgacg
     2341 ctggtggcgt tactggatgt ggacggcgcg ctgttttctg ccgattttcg ctcggcagaa
     2401 cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg ggcgtgcggg taaacagggc
     2461 gaagtggtgc tgcaaacgca ccatccggaa catcctctgt tgcaaacgtt gctctataaa
     2521 ggctacgacg cctttgccga acaggcgctg gctgagcgac gcatgatgca gctgccgccg
     2581 tggaccagcc atgtgattgt gcgtgcggaa gatcataaca atcagcacgc gccgctgttc
     2641 ctgcaacaac tgcgtaatct gatcctttcc agcccgctgg cagacgacaa actgtggatt
     2701 ctcggcccgg ttccggctct ggcacctaag cgtggtggcc gctggcgctg gcagatcttg
     2761 ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta gtggtacgct ggcgcttatt
     2821 aatacaatac cggattcccg caaggtgaaa tgggtgttgg atgtcgatcc catcgaaggc
     2881 taatgcgcaa acgttgcgag gcggatcgaa aaattcaata ttcatcacac ttttcatgaa
     2941 aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat
     3001 ggatgtagta cgcctgacgt accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa
     3061 ctgccgcgac catgaaagac gttgccctca aggcaaaagt ctctacagcg actgtctccc
     3121 gagcattaat gaatcccgat aaagtctccc aggccacccg taatcgggtt gaaaaagcgg
     3181 cccgggaagt gggttattta ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc
     3241 gcaccattct ggtgattgtc ccggatatct gcgatccctt ctttagcgaa attattcgcg
     3301 gtatcgaagt tacggcggca aatcacggat atctggtgct gattggcgac tgtgcgcatc
     3361 aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt gatggcatgt
     3421 tgctgctggg ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt aatctgccgc
     3481 cgatggtgat ggcgaacgaa tttgcaccgg aactggagct gcctaccgtt catatcgaca
     3541 atctgaccgc cgcatttgat gcagtaaatt atttatatga gcaagggcat aaacggattg
     3601 gctgtatagc cggtcccgaa gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc
     3661 aggcgctgcg tcgctgcgga attatggttg atccacaata catcgcccgt ggcgacttca
     3721 ccttcgaagc cggaagcaaa gcaatgcaac agctgcttga tcttccacaa ccgcctactg
     3781 cagtcttctg ccatagcgat gtgatggcgc tcggcgcact ttctcaggca aaacgccagg
     3841 ggctgaaagt cccggaagac ctttccataa tcggttttga taacatcgac ctgacgcaat
     3901 tttgtgatcc gccgctgaca accatcgcgc agccgcgtta cgaaatcggt cgggaagcta
     3961 tgctgttatt gcttgatcaa atgcaggggc aacacgttgg cagtggctct cgtttaatgg
     4021 actgcgaact tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac
     4081 tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg aacgaataaa
     4141 tacagcgaaa cgatagtggc acaacgagat tatgtacgcc gcagccaacc ggcaccttcg
     4201 cggcgaaaaa agagcacctc acggaaaaag caacgaaatc tgcctgcggt ttctcccgct
     4261 atggtcgcta ttgctgccgc cgttcttgtg acctttatcg gtggtctgta cttcattacg
     4321 catcacaaga aagaagagtc cgagacgctg caaagccaga aagtgaccgg aaacggacta
     4381 ccaccaaaac cagaagaacg ctggcgctac attaaagagc tggaaagtcg ccagccgggt
     4441 gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga aaacgccgga gcaactgaca
     4501 ccagaacaac gtcagcttct tgaacaaatg caggctgata tgcgccagca gccaacgcag
     4561 ctggttgagg ttccgtggaa tgaacagacg ccggaacagc gtcagcaaac gctacaacgc
     4621 cagcgtcagg cgcagcaatt agcggagcag caacgcctgg ctcagcagtc gcgtacgact
     4681 gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg
     4741 cgccagtcta aaccagcttc tactcagcag ccgtaccagg atctgctgca aacccctgcg
     4801 cacacgaccg cgcagtcgaa gccgcagcaa gctgcaccgg ttgctcgtgc cgctgacgcg
     4861 ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc
     4921 agaggcgcgg aacaggcaga gacggtacgt gctcagctgg cgttcgaagg atttgactcg
     4981 aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag
     5041 aacgcagaca gcaccctcaa tcggttgaag atggcgggtc atacaaactg cattcggctc
     5101 gccgccgggg gttgaaaccc tcaaaatccc ccccatctat aattgcatta tgcctcgtac
     5161 ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg acaactatag
     5221 taagcgtacg ccgtaacggc catgtggtca tcgctggtga tggtcaggcc acgttgggca
     5281 ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg
     5341 cgggctttgc gggcggtact gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac
     5401 tggaaatgca tcagggccat ctggttaaag ccgccgttga gctggcaaaa gactggcgta
     5461 ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa actgcatcgc
     5521 ttatcatcac cggtaacggt gacgtggtgc agccagaaaa cgatcttatt gctatcggct
     5581 ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg
     5641 cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga catttgcatc tataccaacc
     5701 atttccacac catcgaagaa ttaagctaca aagcgtaagg atctcccatg tctgaaatga
     5761 ccccacgcga aatcgtcagc gaactggata agcacatcat cggccaggac aacgccaagc
     5821 gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc
     5881 gccatgaagt gaccccgaaa aatatcctga tgatcggccc gaccggtgtc ggtaaaactg
     5941 aaatcgcccg tcgtctggct aagctggcga atgcgccgtt catcaaagtt gaagcgacca
     6001 aattcaccga agtgggctat gtcggtaagg aagtggattc tattattcgc gatctgaccg
     6061 atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag
     6121 aactggcaga agaacgtatt ctcgacgtgc tgatcccacc tgctaaaaac aactggggac
     6181 agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg
     6241 aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg
     6301 aaattatggc tcctccaggc atggaagaga tgaccagcca gctgcagtcc atgttccaga
     6361 acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat caaagacgcc atgaagctgc
     6421 tgattgaaga agaagcggcg aaactggtga acccggaaga gctgaagcaa gacgctatcg
     6481 acgctgttga gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg
     6541 gcgagtcttc cggtccggat gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg
     6601 tagaaggttg caccgtttcc accaaacacg ggatggtcaa aactgaccac attctgttta
     6661 tcgcttctgg cgcgttccag attgcgaaac cgtctgacct aatcccggaa ctgcaaggtc
     6721 gtctgccaat ccgcgttgaa ctgcaggcac tgaccaccag cgacttcgag cgtattctga
     6781 ccgagccgaa tgcctctatc accgtgcagt acaaagcact gatggcgact gaaggcgtaa
     6841 atatcgagtt taccgactcc ggtattaaac gcatcgcgga agcggcatgg caggtgaacg
     6901 aatctaccga aaacatcggt gctcgtcgtt tacacactgt tctggagcgt ttaatggaag
     6961 agatttccta cgacgccagc gatttaagcg gtcaaaatat cactattgac gcagattatg
     7021 tgagcaaaca tctggatgcg ttggtggcag atgaagatct gagccgtttt atcctataat
     7081 cgcgttcaat cattttcatc attgtttgat ggggctgaaa ggccccattt ttattggcgc
     7141 gtattatgac tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt ttacgaccta
     7201 aaaccctccc cctcgccttt gctgcaatta tcgtcgggac ggcgctggca tggtggcaag
     7261 gtcattttga tccgctggtc gccctgctgg cactgattac cgccgggcta ttacagatcc
     7321 tttctaacct cgccaatgat tacggcgatg cggtaaaagg cagcgataga cctgaccgca
     7381 ttgggccgct acgcggcatg caaaaagggg tcattaccca gcaagagatg aaacgggcgc
     7441 tcattattac cgtcgtgctc atctgtctct ccgggctggc actggttgca gtggcgtgcc
     7501 atacgctggc tgattttgtc ggtttcctga ttcttggcgg gttgtcgatc attgccgcta
     7561 tcacctacac cgtgggcaat cgtccttatg gttatatcgg tctgggggat atttccgtac
     7621 tggttttctt tggctggtta agcgtcatgg ggagctggta tttacaggct catacattga
     7681 ttccggcact gatccttccg gcgaccgcat gcggcctgct ggcaacggca gtactgaaca
     7741 ttaataacct gcgtgatatc aatagcgacc gagaaaacgg caaaaacacg ctggtggtgc
     7801 gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct gctgatgggc tctctggtat
     7861 gtctggcgct gtttaatctc ttttcgctgc atagcctgtg gggctggctg ttcctgctgg
     7921 cggcaccgtt gctggtgaag caagcccgtt atgtgatgcg ggaaatggac ccggtggcga
     7981 tgcgaccgat gctggaacgc actgtcaagg gggcgttact gactaacctg ctgtttattt
     8041 tagggatatt cctaagccag tgggcagcat aactgacaaa tatcaattaa caattgatga
     8101 ttttgccaac agcccacata gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa
     8161 gcctatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt
     8221 ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt
     8281 aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg
     8341 tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc
     8401 gcgtctggca gtacaaaatg aatgggaagg tctggttatt tacggcgcgg tgcgtcaggt
     8461 agatgacctg gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc
     8521 cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg taaccttctt
     8581 ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag atccgctgga
     8641 tattgaatga taagaaaggc accgcaaggt gccttttttc tgcgttacct gttggcctac
     8701 acagtaaaga aattacgcga aagatgaagc gtaatcagac ctcttccatg cgacccagca
     8761 gggcctgcag acgttcctgc cagccgttct gctgttcttt cagatggttg ttctcacgct
     8821 ccagctcttc gcgctgatgc tgggcatttt gaacttcctg cgacagtgag ttgttttttt
     8881 ctttcagctc ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc tgctgtactt
     8941 ttgcttccag tttctcaaac acttctaatg acattgtcat acctctcctg aattgcaagg
     9001 cgttgatgga taaaaaatcc tcgtcccgat taccggtgac gccttaataa atacgagcgc
     9061 actttagtta gctccgattg tatgaagccg cgccatcgct gtccagcggc acgccttgca
     9121 gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca tcaatcgaaa
     9181 atgttaataa atttgttgcg cgaatgatct aacaaacatg catcatgtac aatcagatgg
     9241 aataaatggc gcgataacgc tcattttatg acgaagcaca cacattttaa gttcgatatt
     9301 tctcgttttt gctcgttaac gataagttaa cagcatgcct acaagcatcg tggaggtccg
     9361 tgactttcac gcatacaaca aacattaact cttcaggatc cgattatgag tcaaacatca
     9421 accttgaaag gccagtgcat tgctgaattc ctcggtaccg ggttgttgat ttttttcggt
     9481 gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt
     9541 gtcatttggg gactgggggt ggcaatggct atctacctga ccgcaggggt ttccggcgcg
     9601 catcttaatc ccgctgttac cattgcattg tggctgtttg cctgtttcga caagcgcaaa
     9661 gttattcctt ttatcgtttc acaagttgcc ggcgctttct gtgctgcggc tttagtttac
     9721 gggctttact acaatttatt tttcgacttc gagcagactc atcacattgt tcgcggcagc
     9781 gttgaaagtg ttgatctggc aggcactttc tctacttacc ctaatcctca tatcaatttt
     9841 gtgcaggctt tcgcagttga gatggtgatt accgctattc tgatggggct gatcctggcg
     9901 ttaacggacg atggcaacgg tgtaccacgc ggccctttgg ctcccttgct gattggtcta
     9961 ctgattgcgg tcattggcgc atctatgggc ccattgacgg gttttgccat gaacccagcg
    10021 cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc
    10081 ggcggcagag acattcctta cttcctggtg ccgctttttg gccctatcgt tggcgcgatt
    10141 gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt tgccttgcga tatctgtgtt
    10201 gtgaaagaaa aggaaaccac aactccttca gaacaaaaag cttcgctgta atatgactac
    10261 gggacaatta aacatgactg aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag
    10321 ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt
    10381 tgagcaaatc tacccaaaac caggttgggt agaacacgac ccaatggaaa tctgggccac
    10441 ccaaagctcc acgctggtag aagtgctggc gaaagccgat atcagttccg atcaaattgc
    10501 agctatcggt attacgaacc agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa
    10561 gcctatctat aacgccattg tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt
    10621 aaaacgtgac ggtttagaag attatatccg cagcaatacc ggtctggtga ttgacccgta
    10681 cttttctggc accaaagtga agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc
    10741 acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg cttatctgga aaatgactca
    10801 gggccgtgtc catgtgaccg attacaccaa cgcctctcgt accatgttgt tcaacatcca
    10861 taccctggac tgggacgaca aaatgctgga agtgctggat attccgcgcg agatgctgcc
    10921 agaagtgcgt cgttcttccg aagtatacgg tcagactaac attggcggca aaggcggcac
    10981 gcgtattcca atctccggga tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg
    11041 cgtgaaagaa gggatggcga agaacaccta tggcactggc tgctttatgc tgatgaacac
    11101 tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc accatcgcct gcggcccgac
    11161 tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg
    11221 gctgcgcgat gagatgaagt tgattaacga cgcctacgat tccgaatatt tcgccaccaa
    11281 agtgcaaaac accaatggtg tgtatgtggt tccggcattt accgggctgg gtgcgccgta
    11341 ctgggacccg tatgcgcgcg gggcgatttt cggtctaact cgtggggtga acgctaacca
    11401 cattatacgc gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc tggaagcgat
    11461 gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa
    11521 caatttcctg atgcagttcc agtccgatat tctcggcacc cgcgttgagc gcccggaagt
    11581 gcgcgaagtc accgcattgg gtgcggccta tctcgcaggc ctggcggttg gcttctggca
    11641 gaacctcgac gagctgcaag agaaagcggt gattgagcgc gagttccgtc caggcatcga
    11701 aaccactgag cgtaattacc gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc
    11761 gtgggaagaa cacgacgagt aatgtaaatg ccggatgaag cgtttatgcc gcatccggta
    11821 gtcctgaaac gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct
    11881 acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa
    11941 ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat
    12001 aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac
    12061 attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt
    12121 ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa
    12181 ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat
    12241 aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt cgggccggga
    12301 gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtagcggcg
    12361 gtgctcggca aaccgttgag cgaactgacg gtaacgattc tagctaaacc acgccacgat
    12421 gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggatggcgac
    12481 gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt
    12541 ggtggcgcgc cggaaggcgt agtttctgcg gcggtgatcc gcgcattaga tggcgacatg
    12601 aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt
    12661 ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgc
    12721 ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc
    12781 gatctgctgg aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc
    12841 cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga tcgcaaagac
    12901 ccggaaatgc aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg
    12961 ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa
    13021 aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg
    13081 tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt caccaagctt
    13141 ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgttaa ctcgcccgat
    13201 aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgattg
    13261 gcggcactga aaccaggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt
    13321 ctggatgaag taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc
    13381 ccttatttat cgattctgca actaggcaaa gatttagatc gcttcaaaaa tctggtcctg
    13441 gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa
    13501 aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg
    13561 tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag cgcgattggc
    13621 ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc
    13681 gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg
    13741 ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga
    13801 gcaaatccgc actcatctta ttgatcatcc cgcgatatgc tggcatcaaa cggttgataa
    13861 atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca
    13921 gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt
    13981 tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg
    14041 ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg
    14101 tcaggtgagc gtcattatgt ctggcgagct ccagagcttt attcaccagt aaggcatctt
    14161 cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa
    14221 gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca
    14281 tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttgtaggc
    14341 cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat
    14401 aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc
    14461 gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac
    14521 atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg
    14581 cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt
    14641 gcttaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc gtggaaataa
    14701 caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt
    14761 cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct tgtgtgccaa
    14821 caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacacccca
    14881 ccgcccattg ccagacaccc ggtaaaatcg cccagttacc cgccagcagc atccacgcca
    14941 caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacagtta
    15001 ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta
    15061 aatttttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa
    15121 tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca
    15181 tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat
    15241 atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc
    15301 tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag
    15361 atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca
    15421 gccagtttcg agaagacttt aacagccaga atcctagcct gccactgaac gaatttcgtg
    15481 ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa attaacgaga
    15541 acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga
    15601 cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct caggaataca
    15661 tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac
    15721 tgcagtcgct gctaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac
    15781 tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta
    15841 agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt
    15901 gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt
    15961 tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa
    16021 actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc
    16081 aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa
    16141 gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc
    16201 cggcgcattc accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat
    16261 catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac tgatcgcgaa
    16321 aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg gtgaaaccga
    16381 agctgaaaac gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact
    16441 gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc
    16501 aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg
    16561 tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg
    16621 ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct
    16681 ggttggcggt gcgtctctga aagctgacgc tttcgcagta atcgttaaag ctgcagaagc
    16741 ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta
    16801 actcttcgcc ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac
    16861 ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc
    16921 gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca
    16981 gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt
    17041 catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt
    17101 tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt
    17161 gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc
    17221 cgtacttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg
    17281 aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat
    17341 attgcagtgg gccattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg
    17401 cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct
    17461 tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg gcagccacaa
    17521 cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt
    17581 acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga
    17641 aaccaccggg tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta
    17701 ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg
    17761 gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc agcgtcgcgc
    17821 ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg
    17881 taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac caccgacacg
    17941 gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt
    18001 tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc aatcccgcgc
    18061 tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac
    18121 agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag
    18181 ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt aggcgtgatc
    18241 accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attgccctta
    18301 cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc
    18361 cactctttat caatccgccc gcgttccgca attgcgtcca cgtcataggc cagagccagc
    18421 gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc tgaaccaccg
    18481 tgtgactgac gcatcaccac gttatcacca gtttgctgtt tccagtgggc gctgaatgcc
    18541 ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata
    18601 tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc
    18661 atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa
    18721 cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg gattaaaaag
    18781 gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg
    18841 cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga attcatgtag
    18901 gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca ggctaaagga
    18961 atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcaccttt
    19021 gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt
    19081 ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc
    19141 catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc
    19201 cagcacagtt gcgcgggttt cacgacctgt ttctttctcg atgaaatgcg ccagttcgtc
    19261 aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc
    19321 cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa
    19381 ttcacagcca ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac
    19441 ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc
    19501 tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt
    19561 gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac gcattgaacc
    19621 catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt ttttcaggtt
    19681 ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag
    19741 gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct gtaccatacg
    19801 gtcttcatac agacccagat agccgtcata aatacccatt acttccagac cttctgtcag
    19861 cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt
    19921 caacacaccg attttcttaa tcatgactac ctctgaactt tggaatgcaa aatgaaatct
    19981 gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa
    20041 tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc
    20101 gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac
    20161 gacggaacag gggtcctgat ggataattac atccgatccc ggaaaacgcc gtaaaatagc
    20221 ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc
    20281 caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc
    20341 gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa
    20401 tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat
    20461 atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa
    20521 ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc
    20581 agcccgcacc gcctggcttt gcgtccggcg caccacccaa cgctgaaacg agacaaggat
    20641 aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat ctatcatcgg
    20701 tgttggagat atcagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat
    20761 aaacatactt tgcgccagcg ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt
    20821 atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat
    20881 atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc
    20941 aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact
    21001 gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac gctagtataa
    21061 cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc
    21121 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    21181 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    21241 gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    21301 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    21361 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    21421 gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    21481 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    21541 catatggctc tgcgtactgc gctgcgtact gcgctgcgta agttcttcac ccggatgcca
    21601 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaagc
    21661 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    21721 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    21781 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    21841 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    21901 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    21961 acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca
    22021 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    22081 gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt
    22141 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    22201 agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttagtgctga
    22261 atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt
    22321 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa
    22381 gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat atgcacattt
    22441 ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc
    22501 gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    22561 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    22621 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcaggggc tgatgattga
    22681 gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    22741 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac
    22801 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    22861 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    22921 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    22981 cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac tgctgattgt
    23041 caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    23101 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga
    23161 actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc agatggtcac
    23221 cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct ctcacgagct
    23281 gcgcaccccg ctgacgcgtc tgcaactggg tacggcatta ctgcgccgtc gtagcggtga
    23341 aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga
    23401 tctgttggtg atgtcacgta atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc
    23461 caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa
    23521 gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg tacggcaatc cgaacgccct
    23581 ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga
    23641 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    23701 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcacg
    23761 cgatcgtgaa tctggcggta caggattggg gctggcgatt gttgaaaccg ccattcagca
    23821 gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg
    23881 gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc
    23941 cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac
    24001 actccggggg ttattttccc cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc
    24061 ggcgcttttg catcgtccgc gtccagcttt tcgataaccc ggcggcggag agtaaacggt
    24121 gatactgatc gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca
    24181 catcgctgac acgggaaacc aactccagcg gcgcgtccgc agacactttc cctggcgcta
    24241 tcacgctata caaccacccc accttacctg tgttttgcat caactgcgct atatcgctga
    24301 tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg
    24361 cctctcccca gcggaaaata tcgcccatat aaacattact ttccgtcagg ccgtcggttg
    24421 agaggttttc accaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgcg
    24481 cccagtagag ataatgctca cgaggataat gacacagcgc tctgtctggc ccaccatgaa
    24541 cctttttctc cgcctgctcg tcaccttcca gccccagctc cgtcagcatc aactcaccat
    24601 caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg
    24661 tgtatacatc aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac
    24721 catatttatt ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca ggaactcaat
    24781 agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc
    24841 accgtaccta aaatttcgat tttcgctgct ctggctttga ctttacgtga ccagatagag
    24901 gtaagaattg gcaccagaat cgaagtcaca atgaccgccg tcgctaccag cgaagttgct
    24961 gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcatagg tgtcgctacc
    25021 gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat
    25081 ttatcggcga taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc
    25141 cccagcaaac cagtctgggc aatcacagtc aaatcaatgg tattgcccag cgcgaaggca
    25201 aagaatggaa tcagcgtttg caccgctttg ctgaaaaatt ctcgtaattc agggtcaagg
    25261 ttccccaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca
    25321 aacgaggcaa tccctgcagt gcccagaata atcatcgtca tgagcggccc ggactccaac
    25381 gacatcaaca caaatgcccc agcttcttct tttgtgccgt actgctgcat gatggaagcg
    25441 taaagtccgc cattggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca
    25501 aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc
    25561 cacgcgacgg caatttttgt taccaccagc gtaccggatt tacgcaatac cgttcccgtc
    25621 gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat ggacaccgta
    25681 ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg
    25741 tggcacagtg cgccaaggaa tagcgggacg agcatcatcc ccccccggga ttttctcaat
    25801 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
    25861 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
    25921 gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt atcggatcat tgtttttatt
    25981 ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
    26041 gcggcgtaaa cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcgaaacgtg
    26101 ccgctgcttc atcccagttc accacgttcc agaactcttt gatgtagtcc ggacggcggt
    26161 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    26221 aagcgccaga aatagcttca cccatcagcg gggaatcctg gttagcagta gaaaccaccg
    26281 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
    26341 ctttctcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
    26401 tcaggtcacc ctgtaaggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
    26461 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    26521 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
    26581 tggcgttgtt gacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    26641 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
    26701 tcatctccag tattgtcggg cggccgattg ttaatgcgcg taagcagttg gttcattata
    26761 gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcgtaag ggtatggttt
    26821 tgcaggaaaa ttcccgagat gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa
    26881 atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc acgattttta cactcatctc
    26941 gtcggagatg tgacgcgacg aaaaatgatg aggataagaa gatgagtaac gcgattacga
    27001 tggggatatt ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac gctccgttca
    27061 aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt tggtgggatt gtttcgtgga
    27121 ttattctgcc gtgggccatc agcgccctgt tactgccgaa tttctgggcg tattacagct
    27181 cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta
    27241 atatcaacta cggcctgacc atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca
    27301 ttggcattac gttgattgtc ggtacgctga tgacgccaat tatcaacggc aatttcgatg
    27361 ttctgcttaa taccgaaggc ggacgcatga cgttgctcgg cgttctggtg gcgctgattg
    27421 gtgtagggat tgtgactcgc gccgggcagt tgaaagagcg caaaatgggc attaaagccg
    27481 aagagttcaa cctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt ttctctgccg
    27541 gtatgtcttt tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc gcactgggcg
    27601 tcgatccact gtatgtcgct ctgccaagct atgtcatcat catgggcggc ggcgcgatca
    27661 tcaacctcgg tttctgcttc attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg
    27721 acttctcgct ggcaaaaccg ctgatcattc acaatgtgtt actctcagca ctgggcgggt
    27781 tgatgtggta tctgcaattc tttttctatg cctggggcca cgcccgcatt ccggcgcagt
    27841 atgactacat cagttggatg ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg
    27901 ggctggtgct gaaagagtgg aacaatgcag gtcgccgtcc ggtaacggtg ttgagcctcg
    27961 gttgtgtggt gattattgtc gccgccaaca tcgtcggcat cggcatggcg aattaatctt
    28021 tctgcgaatt gagatgacgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca
    28081 ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc
    28141 ggctatgctg gagaagatat tgcgcatgac acactctgac ctgccgcaga tattgattga
    28201 tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct
    28261 catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccagc cgggtaatca
    28321 gcttatccag caacgtttcg ctggatgttg gcggcaacga atcactggtg taacgatggc
    28381 gattcagcaa catcaccaac tgcccgaaca gcaactcagc catttcgtta gcaaacgaca
    28441 catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgc gccatcccca
    28501 cgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgctccct
    28561 gccagtcaag attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat
    28621 cgttaacgga agcgtaggag tgtttatcgt cagcatgaat gtaaaagaga tcgccacggg
    28681 taatgcgata agggcgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca
    28741 gctcacaaaa atcatgtgta tgttcggcaa aaacatcttg cggataacgg tcagccacag
    28801 cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca
    28861 ccgtggctac ctcggccaga gaacgaagtt gattattcgc aatatggcgt acaaatacgt
    28921 tgagaagatt cgcgttattg cagaaagcca tcccgtccct ggcgaatatc acgcggtgac
    28981 cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccgcagcga
    29041 taggcgatgt cagtaacgct ggcctcgctg tgacgtagca gatgtcgggc cttcatcagt
    29101 cgcaggcggt tcaggtaacg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt
    29161 agcgtacgca gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca
    29221 aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc
    29281 tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg
    29341 gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc
    29401 acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagttct
    29461 tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc
    29521 acattggtca gacacagatt atcggtatgt tcatacagat gccgatcatg atcgcgtacg
    29581 aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg
    29641 ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc
    29701 gggagccggg gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc cacactatgt
    29761 aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca
    29821 ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac
    29881 agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaatgaagc aaattgtgaa
    29941 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
    30001 ggtcacgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat
    30061 gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc
    30121 gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg
    30181 cctgcatagc cagaacggtt atgtcacctg gaatgtggat agcctggaaa gtgccattcg
    30241 ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac
    30301 ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc
    30361 ttatcgcgat agccgctcca atggcctaat ggcgcaggca cagcaacaac tcggcaaacg
    30421 cgatatttat caacgtagcg gcatccagtt tctgcccttc aatacgcttt atcaactgcg
    30481 tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc
    30541 ggattacttc agctatcgcc tgaccggcaa gatgaactgg gaatatacca atgccaccac
    30601 cacacaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg
    30661 ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat
    30721 ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc
    30781 ggttatcgcc tcgccgttaa acggttcacg cgctgcttat ctctcttctg gcacctggtc
    30841 attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat
    30901 aaccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg
    30961 gctgcttcag cgagtgcttc aggagcggca aatcaacgat ctcccggcgc ttatcgccgc
    31021 gacacaggca cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa
    31081 tcctgaggcg atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc aaccgatccc
    31141 ggaaagtgat gctgaactgg cgcagctacg cggtgaagat ttctcgcaac tgcatattgt
    31201 cggcggcggc tgccagaaca cgctgctcaa ccagctatgt gccgatgcct gcggtattcg
    31261 ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat atcggcatcc agttaatgac
    31321 gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct
    31381 gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc agcttcactc
    31441 tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc ctgggagctg
    31501 gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg ccaacttgat
    31561 cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt tgaaaacccg
    31621 gaaggttcgc tgaccggggg gattcaggcc acaggcaatt atccgggcaa agcgcgtaat
    31681 gccagtgagc tacgtgccga tctggaacag gctatgcggc tgattccggg gccgaaacgg
    31741 cttaatttcc atgccatcta tctggaatca gatacgccag tctcgcgcga ccagatcaaa
    31801 ccagagcact tcaaaaactg gggtgaatgg gcgaaagcca atcagctcgg tctggatttt
    31861 aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctctc gcatcccgac
    31921 gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt ttcggcctat
    31981 tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga tggtatgaaa
    32041 gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact ggatgaggtg
    32101 atcagcaaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa attgtttggc
    32161 attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta tgccaccagc
    32221 cgccagactg cgctgtgcct agacgccggg cacttccacc cgactgaagt gatttccgac
    32281 aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt
    32341 cgctgggact gcgatcacgt agtgctgctg gatgatgaaa cccaggcaat tgccagtgag
    32401 attgtgcgtc acgatctgtt tgaccgggtg catatcggcc ttgacttctt cgatgcctct
    32461 atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt
    32521 gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga ttacactgcg
    32581 cgtctggcac tgctggaaga gcagaaatcg ttgccgtggc aggcggtctg ggaaatgtat
    32641 tgccaacgtc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg ggcttatgaa
    32701 aaagaaattt tgagccagcg tgggtaaaca ctgccggatg cggcgcgagc gccttatccg
    32761 gcctacgggt cgtgcatctg acaacaccga atttacagga acacagaaca tgcaaaacat
    32821 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg
    32881 ctgggacgaa cgcaacggcg gcaacctgac gctgcgcctg gatgacgccg atatcgcgcc
    32941 ttataaagac aacgtccatg cacaaccgcg ctatatcccg ctcagccagc ccatgccttt
    33001 actggcaaat acaccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
    33061 cgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gatggcgcgg gctaccatat
    33121 tctctggggg ttaaccaacg aagccgtccc gacatccgaa ctcccggcgc actttctctc
    33181 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
    33241 caccaacctg atcgccctca cctatgtact ggaaaacgac accgcggtct ttactcgcca
    33301 actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc
    33361 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
    33421 ttcgctggtg ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac
    33481 attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
    33541 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
    33601 cgttacacca ctagccagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
    33661 tgtcatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg
    33721 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    33781 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc
    33841 ggtagccagt actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
    33901 taacccggat aacagcccgg tgagtagcga gctgca
//
LOCUS       DAJVTY010000025        32308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVTY010000025 DAJVTY010000000
VERSION     DAJVTY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32308
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.025"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            197..991
                     /gene="panB"
                     /locus_tag="OQQ66_001094"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="OQQ66_001094"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1904101.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="OQQ66_001095"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="OQQ66_001095"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1904102.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALSR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OQQ66_001096"
     CDS             complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OQQ66_001096"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1904103.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="OQQ66_001097"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="OQQ66_001097"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1904104.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4900)
                     /locus_tag="OQQ66_001098"
                     /pseudo
     CDS             complement(3700..4900)
                     /locus_tag="OQQ66_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4995..5435)
                     /locus_tag="OQQ66_001099"
     CDS             complement(4995..5435)
                     /locus_tag="OQQ66_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1904105.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5540..6310)
                     /locus_tag="OQQ66_001100"
     CDS             complement(5540..6310)
                     /locus_tag="OQQ66_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1904106.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6307..7233)
                     /locus_tag="OQQ66_001101"
     CDS             complement(6307..7233)
                     /locus_tag="OQQ66_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1904107.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7342..8004
                     /gene="can"
                     /locus_tag="OQQ66_001102"
     CDS             7342..8004
                     /gene="can"
                     /locus_tag="OQQ66_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1904108.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="OQQ66_001103"
     CDS             complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="OQQ66_001103"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1904109.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8787..11177
                     /gene="gcd"
                     /locus_tag="OQQ66_001104"
     CDS             8787..11177
                     /gene="gcd"
                     /locus_tag="OQQ66_001104"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1904110.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="OQQ66_001105"
     CDS             complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="OQQ66_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1904111.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12940..13287
                     /locus_tag="OQQ66_001106"
     CDS             12940..13287
                     /locus_tag="OQQ66_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1904112.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13392..14258
                     /gene="speE"
                     /locus_tag="OQQ66_001107"
     CDS             13392..14258
                     /gene="speE"
                     /locus_tag="OQQ66_001107"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1904113.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14274..15068
                     /gene="speD"
                     /locus_tag="OQQ66_001108"
     CDS             14274..15068
                     /gene="speD"
                     /locus_tag="OQQ66_001108"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1904114.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15105..15467)
                     /locus_tag="OQQ66_001109"
     CDS             complement(15105..15467)
                     /locus_tag="OQQ66_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1904115.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15642..18239)
                     /gene="acnB"
                     /locus_tag="OQQ66_001110"
     CDS             complement(15642..18239)
                     /gene="acnB"
                     /locus_tag="OQQ66_001110"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1904116.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18594..20447
                     /locus_tag="OQQ66_001111"
     CDS             18594..20447
                     /locus_tag="OQQ66_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS1904117.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20518..21942)
                     /gene="lpdA"
                     /locus_tag="OQQ66_001112"
     CDS             complement(20518..21942)
                     /gene="lpdA"
                     /locus_tag="OQQ66_001112"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1904118.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22267..24159)
                     /gene="aceF"
                     /locus_tag="OQQ66_001113"
     CDS             complement(22267..24159)
                     /gene="aceF"
                     /locus_tag="OQQ66_001113"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1904119.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24174..26837)
                     /gene="aceE"
                     /locus_tag="OQQ66_001114"
     CDS             complement(24174..26837)
                     /gene="aceE"
                     /locus_tag="OQQ66_001114"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1904120.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26998..27762)
                     /gene="pdhR"
                     /locus_tag="OQQ66_001115"
     CDS             complement(26998..27762)
                     /gene="pdhR"
                     /locus_tag="OQQ66_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1904121.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28303..29676
                     /gene="aroP"
                     /locus_tag="OQQ66_001116"
     CDS             28303..29676
                     /gene="aroP"
                     /locus_tag="OQQ66_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1904122.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29719..30573)
                     /gene="ampE"
                     /locus_tag="OQQ66_001117"
     CDS             complement(29719..30573)
                     /gene="ampE"
                     /locus_tag="OQQ66_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1904123.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30570..31121)
                     /gene="ampD"
                     /locus_tag="OQQ66_001118"
     CDS             complement(30570..31121)
                     /gene="ampD"
                     /locus_tag="OQQ66_001118"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1904124.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31209..32102
                     /gene="nadC"
                     /locus_tag="OQQ66_001119"
     CDS             31209..32102
                     /gene="nadC"
                     /locus_tag="OQQ66_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1904125.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGDGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
BASE COUNT         7790 a         8786 c         7964 g         7768 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaagcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gccttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctcg gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccata
     4561 atggcgcact gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaattaagt
     4621 tctgattact cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct
     4681 gaactggctc gagatgccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt
     4741 aataacttgc ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg
     4801 tcaccatctt gtcgccaatt tgcgcccaga tcgcagcatt ttcgatggtt tgcatataac
     4861 gggcaggtaa cgcggcacta acgcttgcgg taaacagcat cagcaggatg aataacagct
     4921 tgtttataca tgaaaaaata accgagggtg aaacaacgct tgtattgtag caaaagcgcc
     4981 atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacaaaagg cggatttttt
     5041 tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga
     5101 aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg
     5161 acaccagaaa tcacttcaca acgggagtgt ttatggctta ataatgaagc cacgcggtac
     5221 ggtggcgctc cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc
     5281 agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca gaaattcacg
     5341 gcccggcact gaagaagtgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc
     5401 cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca
     5461 gtctatcctg accattgttg ataaagggtg atagccgtca aaactcctct cccccgaatc
     5521 cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac
     5581 agatcaaata aaacaccaca ataaagacca ccagtacgcc aaaggtagtg gccagcggca
     5641 catcatttat accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca
     5701 ggtgcgacag cccttgccag aacggcggca acaaagtcag ggagtaaaac accccgccca
     5761 aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg
     5821 caaacacgcc gttcagcaaa cccgcaaggg agaatagcac cgccgtgagc accagggtta
     5881 aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa attgccgtca
     5941 ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga
     6001 cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa
     6061 aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac cgacatcatg atcagccccg
     6121 gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc aatacgcgaa cctatcaggt
     6181 taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc
     6241 agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa
     6301 gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag
     6361 acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa
     6421 tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag
     6481 tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa
     6541 ggtttccaat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc
     6601 gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtagt
     6661 gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg
     6721 aagttcaata tccacgcctg cggtcggttc gtcgagaatc agtagtttag gttcatgcat
     6781 taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatac gcgcacgttc
     6841 gttgcgtttt ccccataaat cgagttgttt gagatatttt tcgctgcgga tgtacgcttc
     6901 tttgcgctcc acgccgtagt accctgcctg attcaccaca atttgctgca cggtttcaaa
     6961 cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc
     7021 cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct
     7081 gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc
     7141 acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt
     7201 tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac
     7261 actttatctg tggtttaaat cgtcccggag ttaccctata ttagccaaac gtaattattt
     7321 ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta
     7381 tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa
     7441 aaaccgcact ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc
     7501 ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc
     7561 gacctgaact gcctttccgt ggttcagtat gcagtggatg tactagaagt tgaacacatt
     7621 attatctgtg gccactacgg ttgcggcggc gtacaagccg cagttgaaaa cccggaactg
     7681 gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg
     7741 ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa
     7801 caggtgtata acctaggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa
     7861 gttaccattc acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtt
     7921 actgccacca accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag
     7981 ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc
     8041 acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg taacgctgtg
     8101 cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg ataaattcta
     8161 ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc gccagcgact
     8221 tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga
     8281 tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt
     8341 tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt
     8401 catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca
     8461 gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca
     8521 gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca
     8581 tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca
     8641 acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa
     8701 agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata
     8761 ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac
     8821 tcgtcacgct aacagtcctt tttgcagcgc tttgcgggct gtatctactc attggcggag
     8881 gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt gtgatgctcg
     8941 gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc
     9001 tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc
     9061 cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc
     9121 gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg
     9181 gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg
     9241 ccgatgccac acctacggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg
     9301 gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc
     9361 ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg
     9421 gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg
     9481 ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg cattacgatc
     9541 ctaagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcgtc tcttatcatg
     9601 aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc
     9661 ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa
     9721 ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc aaaccgggtc
     9781 tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag
     9841 ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca
     9901 ccggggagct gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg
     9961 acgaacacac ctttaccttt aactcgccaa actcctgggc accagcggcc tatgacgcga
    10021 agctggatct ggtctatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc
    10081 gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc actaccggga
    10141 aactggcgtg gagctaccag accgttcacc acgacctgtg ggacatggat cttccggcac
    10201 agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg
    10261 cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg cgaactggtg gttccggccc
    10321 cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta cgttactccg acccagccgt
    10381 tctctgaact gagcttccgt ccgaagaaag atttgagcgg tgcggatatg tggggagcca
    10441 ccatgtttga ccaactggtg tgccgcgtga tgttccacca gatgcgctat gaaggcattt
    10501 tcacgccgcc atctgaacag ggcacgctgg tcttcccggg taacctggga atgtttgaat
    10561 ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca atggcactgc
    10621 cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg
    10681 ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca
    10741 cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa acagccagca tggggttata
    10801 tctcggcgct ggatctgaaa accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc
    10861 aggacagtat gccgttcccg atgcctgtac cggtgccgtt caatatgggt atgccgatgc
    10921 tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact
    10981 acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg
    11041 gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg
    11101 caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgccctgc
    11161 cggatgatgt gaaataacca taatgcccgg agagatccgg gcatatttcc gaatacggtc
    11221 tttttatacc gtaaacccta acatcatccc cgtatcttca tgctccagca gatggcagtg
    11281 cgccatataa gcatgttctt tcggtgcgtc gtgattaaac ttcaccagca cttcactgac
    11341 attgccttct accttaacgg tatctttcca gcccgcgcga tgagccgctg gcggtttgcc
    11401 attttctgac aagatacgga actgcgtgcc gtgaatatgg aacggatgca gcatcatgtc
    11461 gcccacgcca gagataaccc aacgttcgta ctgccctttc gccgccgcaa acatcggctt
    11521 attcatatca aacgcctgac cgttgatttt attggcatgg tggaaatcga acttcccgcc
    11581 gtggttcata tgattcatat taccgtgccc catatggccc atcatctggc tgtgatccat
    11641 tccggccatc gcctgatcgc catatttctc cattagcatc tgcataccca tcatatcgag
    11701 catcgggtcc atagaaagtt gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc
    11761 aggcaggcta cttaatgtgt ctggcaaagc accggaggca ctaatagcaa tcggctgaat
    11821 ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg
    11881 tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc accagcactt caaaacgctc
    11941 gcccatcagc accggcagct cgctcacctt caccggttca ggtagcagac caccgtcgct
    12001 ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca
    12061 gccattgagc aaacgcaggc gcagccaacc acgcggggca gcgtgttgtg ggtagattgc
    12121 accgttggtc agcagcgtat cgccaaacca gcccacggcg gcggtcatca catccagttg
    12181 ataatcaatc tgcccatcag cgttaaattt tttatcctga acaatcaccg gaacatcatc
    12241 gataccccac tgttttggca gcattaattt caggatctcg tcatcttcaa ttaccaccag
    12301 cccagccaac cccatcgcca cctgtcgccc agtcttgcca tgttgatgtg gatggaacca
    12361 gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag cgcttgccac ctggcggaat
    12421 aattccctgc gggccgccgt cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt
    12481 cgtctcttcc gtcagttggt tatagatatc aaccgttacc gctttgccgc gctgtaattt
    12541 caccgccggc cccagcagat tgccgttata gccccaggta gttgcagttt tctcgccaaa
    12601 ggtagactga cctgcgccaa tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa
    12661 atcagggatc ggtaacgttg ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa
    12721 ggctgaagcc acacccagcg cgacggaata ttttaagaaa tcacgacgtt gcatagttat
    12781 ttccttattc ttaagcaggc gaaacaaaat caaacgttga gcatagtcct tccccttacg
    12841 ggaaggtcaa gccgcagaca caatttaatc gacaataatg gtcgtcgcct cgctcgcagt
    12901 gtgctaacgt ttatcttctt taagcccctg gtagaagcaa tgaagacgtt tttcagaaca
    12961 gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa
    13021 gccgaagata tggccgattt aacggcagtt tttgtctttc tgaagaacga ttgtggttac
    13081 cagaacttac ctaacgggca aattcgtcgc gcactggtct ttttcgccca gcaaaaccag
    13141 tgggatctca gtaattacga caccttcgac atgaaagccc tcggtgaaga cagctaccgc
    13201 gatctcagcg gcattggcat tcccgtcgct aaaaagtgca aagccttggc ccgcgattcc
    13261 ttaagcctgc ttgcctacgt caaataatcc ctgatacctt tttgtagaaa tattgaccgt
    13321 gcatccgcgg tcaatgttag ctattatgtt gcgccctttt ttacgggtgt taacaaagga
    13381 ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc
    13441 agtactttgc ggtagataac gttctgtatc atgaaaagac cgatcaccaa gatctgatca
    13501 tttttgagaa cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg
    13561 agcgcgacga gtttatctat catgagatga tgacccatgt tccgctactg gcccacggtc
    13621 acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc
    13681 gacataaaaa cgttgagtca atcacgatgg tagaaatcga tgcgggtgtc gtgtcgttct
    13741 gccgtcagta tctgcccaac cataacgccg gtagctacga cgatccgcgc tttaagctgg
    13801 tgatcgacga tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct
    13861 ccgactgcac cgatcctatc ggtcccggcg aaagtctgtt cacctcagca ttttatgaag
    13921 gctgcaaacg ttgcctgaat cctagcggta tcttcgtcgc acaaaacggc gtctgctttt
    13981 tacagcagga agaagccatc gacagccatc gcaaactcag ccattacttc agcgacgttg
    14041 gcttttatca ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga
    14101 cagataacga cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt
    14161 ctggcctgaa atgccgttat tacaatccgg caatccatac ggcagctttt gccttacctc
    14221 agtatctgca agatgcactg gcttcacagc cgtcctaagg agaatataag aaattgaaaa
    14281 aactgaaact gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata
    14341 tctgctacgc caaaactgcc gaagagcgcg acggttatat tgcttatatc gatgaactct
    14401 ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata
    14461 ttcttaacat cgcccgccag gattacgaac cacagggtgc cagcgtcact attctggtga
    14521 gtgaagaacc ggttgacccg aaactcatcg acaaaacaga acaccccggc ccactgccag
    14581 aaacggtcgt tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagcc
    14641 atcctgaagg tggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga
    14701 tttctccgct gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca
    14761 ttgattatcg cgtgcgcggt tttacccgcg acattaacgg tatgaagcac tttatcgacc
    14821 atgagattaa ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg
    14881 tggatgtaaa cgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg
    14941 accttaagca ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg
    15001 aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag
    15061 ctgtttaacg gctctggcgg agctccaggc tccgccagat ttatttactt ctgctgcacg
    15121 aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag cgacaggctt
    15181 tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc cagttgattg
    15241 gcgcgaacca tcacctcttc accgtccatc cacagggtgt attcatgccc tgcccgttgc
    15301 caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc aagcaaggcc
    15361 aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc catggacata
    15421 cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt gtgttcctct
    15481 gctcccggca atgcgccgaa atcgcattgc gccattgatg taattatcgc agccttaccg
    15541 gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa tctcattcac
    15601 cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc agtctggaaa
    15661 atcaccccat cggctttttc ggtgtactga gaaagttggt tgaagttcag ataacgataa
    15721 gtatcaacgg cagttttatc tacttgcgca acgtaggtct ggtactcttc cggcgtcggc
    15781 agtttgccaa tcagcgccgc aacagccgcc agttctgcag aagccaggaa gacattcgcg
    15841 ccagtcccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct
    15901 gctacacgcg cctggttacc catacacaga gaacagcccg ggatctcgat acgagccccg
    15961 ctcttaccga agacgctgta gtagccttct tcagtcaact gtgcggcgtc catacgggtt
    16021 ggcggtgcca cccacaggcg ggtcggcaac tggcctttat gcgcatccag cagtttaccc
    16081 gcagcacgga agtgaccgat gttagtcatg caagaaccga taaatacttc gtcgatcttc
    16141 tcgccctgta ccgcagacag cggacgcgcg tcatccgggt cgttcggagc acacaggatt
    16201 ggctctttaa tatccgccag atcgatgtca atcactgccg cgtattccgc atctgcatct
    16261 gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg
    16321 gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat attagagtta
    16381 aggtattcga tgattggttc tttgttcagc ttgatagtac aaccagcggc agaacgctca
    16441 gcggacgcat cggtaagctc aaaggcctgc tcaactttca gatccggcag accttcaatt
    16501 tccagaatgc ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct
    16561 tgtttgatgg catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc
    16621 attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt
    16681 gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg
    16741 gtatgggagt cacctccggt accgacggta tccggcagca gcatacggtt cagccaggag
    16801 tggattacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc
    16861 agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac
    16921 tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc
    16981 atcgggccgg tagtgtcctg tgaacctaca gaagtcattt tcggttcgca gtacgcgccc
    17041 ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg
    17101 cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt
    17161 gcttcacgcg ctttggtggt caggccacgc ccgataatca gcgggatacg gccgccagca
    17221 cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgcctgtt
    17281 tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg
    17341 ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg
    17401 ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata
    17461 tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc
    17521 acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg
    17581 atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc
    17641 gccagcgcgt gcagtgggat atccgggcgc gaccacgcat ccggtgccgg agaaaggtca
    17701 tcggtgttgg tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc
    17761 gggcgattca gaaaccattc ggcatccgcc caggactgca ttacctgctt cgcatattcg
    17821 ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag cagtgtgtga
    17881 gaaagtgctt tggcagcgat cggtgccagt ttggcatcat ccagcgcgtc gatcagcgga
    17941 tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc cggagtcagc
    18001 agaggggatt tggcttcgcc tttcgcgata gcagccagga agcctgcttt gacataggcg
    18061 gcttcatcga cgccaggggg aacacggttg gttaacagat ctaacaggaa ttcttcttcg
    18121 cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc atccaggggt
    18181 ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg
    18241 acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga
    18301 gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac
    18361 gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg
    18421 tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc
    18481 tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact cggattaaca
    18541 gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa
    18601 tgactttgcc gtttaaaccc catgtgctgg cactaatttg cagtgccggg ctttgtgccg
    18661 cctctgccgg gctatatata aaaagccgca cagtggaagc ggctgtggaa acgcaatcga
    18721 cacaactggc tgtgtctgac gctgccgcgg ttacgcttcc tgcaacggtt tccgcgcctc
    18781 ccgtaacacc cgccgtcgtt aaatccgcat tcagcactgc acaaatagat caatgggtcg
    18841 cgcccgtcgc gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc
    18901 cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg
    18961 ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct
    19021 ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg
    19081 cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg caactggcgc
    19141 aacaaaccgg ttcgctgaag tcatcaaacg aacaaaaagt tattaccaca acgaagaaag
    19201 ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc gttttaactg
    19261 ccagccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc gccaatcctg
    19321 atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg
    19381 cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttattgac agctttgtgc
    19441 gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc actattcagc agcatcgact
    19501 gggatgacga cgatcatgac catcatcatc atgacgatga tgattatcat caccacgatg
    19561 gcggtcatcg tgacggtaat ggctggcaac acaacggcga caacatcaat atcgacgtca
    19621 acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca
    19681 atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc
    19741 aaaccgatgt caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc
    19801 agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac
    19861 gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct
    19921 atgacgactt ccgcgacttc agccgtcgcc aacccctgac ccagcaacaa aaggacgccg
    19981 ctcgtcagcg ttatcagtca gcttctcctg agcagcgcca ggcagttcgc gagagaatgc
    20041 agactaaccc gcagatccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct
    20101 cgcctgagca gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc
    20161 gaagagacgc agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta
    20221 aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg
    20281 ttcaatcagc atcacctgaa caacgtcagg tttttcggga gagagttcag gagagccgcc
    20341 cacaacgtct aaacgacagt aaccgtactg ccagattgaa taacgatcaa cggtcagcag
    20401 tacgcgaacg tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acagacgtaa
    20461 aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta
    20521 cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc
    20581 caggcccaca gactcgtgca gagtcgggtg cgcatggatg gtcagcgcga tgtcttcagc
    20641 gtcacaaccc atttcgattg ccaggccgat ttcacccagc agctcgccgc cgttggtacc
    20701 gacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc
    20761 gtctgcgcag tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc
    20821 atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg
    20881 ttcggtatag gcgatagacg ggataacttt cggatcgaag tagtgttttt taccggcgat
    20941 aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccaac
    21001 gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat
    21061 gaaaccacgg tcgtccactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg
    21121 cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagcgg gtgctttttt
    21181 gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc
    21241 cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc
    21301 agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg
    21361 gtatacggtg cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg
    21421 tacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt catgcggaat
    21481 aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac
    21541 ggttttaccg ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc
    21601 gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt
    21661 gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt cgccgaagac
    21721 gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc
    21781 tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac
    21841 gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc
    21901 tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt
    21961 atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt cacgccctca
    22021 ccccagtcac ttacttatgt aagcccctgg ggattctggc gcttgctacc ttgctgcacc
    22081 cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt tctatccgtc
    22141 ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc
    22201 gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc
    22261 ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa
    22321 acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat
    22381 cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc
    22441 gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc
    22501 gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg
    22561 cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac
    22621 gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac cgatgttgat
    22681 gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga agcgaggcat
    22741 ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga tcttcacatc
    22801 cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat
    22861 atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc tcaggttagc
    22921 accagaaatt ttctggatac ggcccagttc cacttcttcg atttcaccaa acttgctgaa
    22981 gtccaccttc ggccacggca gcatgcctgg gataccgccg ccagtcgccg ccggagctgc
    23041 ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc
    23101 tttacggcca gtgcccttca ctttcgccag gttaacacca aactcgcgtg ccagacggcg
    23161 gatcagcgga gtcgcgtgaa cataagcgtc gttttcagca aattcagatt tgccttccgc
    23221 tttcgcagct ggtgctgctg ccggggcttc agcttttgct gccggtgccg gcgctgccgc
    23281 ttcctgtttc gcaggagctg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag
    23341 cgagccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa
    23401 cggcgccgga acttccatag aagctttgtc gccttctacg gtgatcagtg actgttcagc
    23461 ggcaactttg tcgcccactt tcaccatcac ttcagtcact tcaacttcgt caccgccgat
    23521 atccggaacg ttaacttctt tcacgccagc tgctggtgca ggggccgctg ccggagccgc
    23581 ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat
    23641 cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca cggtgccagc
    23701 aaacggagcc ggaacttcca tagaagcttt atcgccttct acggtgatca gcgactgttc
    23761 agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc
    23821 gatatccgga acgttaacgt cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc
    23881 ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa
    23941 aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac
    24001 gatacccgcc tgcggagacg gaacttccat agaggctttg tcgccttcta cggtgatcag
    24061 cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc
    24121 atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc
    24181 agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact
    24241 ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa
    24301 gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca
    24361 gtacccagta cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt
    24421 ttcatatagt cggtagatgc cactgccgga gcgtcgttca tcacctgagc gatatacgga
    24481 acgcgcggag tttccagcgg atgaagcatg ttccagcgtt cacaatcctg accatcacgc
    24541 gccagctcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc
    24601 aggatctctg ccgcttcacg gacgtgacgc aggatagaac cggagcccag cagctgaact
    24661 ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca
    24721 gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtggtgat gtagtagtaa
    24781 acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact
    24841 tcgtaagcgt aagccgggtc gtaagagata cagttcggaa tagtcagcga ctgaatgtgg
    24901 ctgtgaccat cttcatgctg cagaccttct ccgttcaggg tggtacgacc ggaagtaccg
    24961 ccgatcagga agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccaatacgc
    25021 tggaagccga acatcgagta atagatgtag aacgggatca tcggcagatt gttggtgctg
    25081 taagaggtcg ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc
    25141 agaatctgac ctttctcatc ttctttatag taagcaacct gctcgcggtc ctgcggggtg
    25201 tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa
    25261 gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc
    25321 attacgttca gggcacgaac gaaagcgata gtggtagaga tctctttgct ctgctcttcc
    25381 agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga
    25441 cggcttggca gataaccgtg cagtttctga cgctgtgcgt gcagataggt atgctcttca
    25501 gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca
    25561 ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg
    25621 atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggaatg agcaaggatt
    25681 actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga
    25741 tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct
    25801 gcggtttcag gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg
    25861 aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta
    25921 tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca
    25981 gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg
    26041 tcaagacgct gcaggttaca gttgataacg aagaccaggt tatccagttt ttcacgggta
    26101 gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg
    26161 taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta
    26221 gcctggtaaa tagcaccaat cggccccagc cccatagata cggtcgggaa ctgccagaat
    26281 tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg
    26341 aagttatcca gctgctcctg agtcagacga ccttccagga aagcacgagc gtacacgccc
    26401 ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg
    26461 aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga cgccatatgg
    26521 ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc gttccagcgg
    26581 atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca
    26641 acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt tacaccgcct
    26701 ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca
    26761 cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt
    26821 gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac
    26881 ctgtcccatt gaactctcgc cggaaagctc aataagacag gttctacgtt tagttgccgc
    26941 gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccagt aaaatcacta
    27001 attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt cacgacttct
    27061 gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc
    27121 cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag
    27181 catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg
    27241 ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcag tgacagcaat
    27301 ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagttc
    27361 tatggcgtga tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc
    27421 gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgactc
    27481 aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata ggctgctctg
    27541 gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat
    27601 cgcctcacgc aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg
    27661 gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat
    27721 cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc
    27781 acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta
    27841 ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac
    27901 ttgacatata taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa
    27961 gggatttttt aacgattatt aaactataaa aatccgaatt gaaccgattc acttaccaat
    28021 tttgtgattt ttaattcaat taaaacgaat ttaaattcat tctacatatt gagaggggtt
    28081 gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc
    28141 gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat
    28201 tcaaacttct ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc
    28261 atcactgcgt agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag
    28321 cacggcgagc agctaaaacg cggccttaaa aaccgccata ttcagcttat cgcgctgggt
    28381 ggcgcgatag ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca
    28441 gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg
    28501 ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac
    28561 tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt
    28621 gccatggctg agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc
    28681 acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt
    28741 aaagtgtttg gcgagatgga gttctggttt gccattatca aagttatcgc tgtggtagcg
    28801 atgatcatct tcggcggctg gctgctattc agtggcaaca gcggcccgca ggcgaccgtt
    28861 agcaacctgt gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg
    28921 atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa
    28981 gctgataacc cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg
    29041 attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc
    29101 gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat
    29161 gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc
    29221 aacagccgta tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct
    29281 gtcgataaac gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg
    29341 tgcgtactga ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg
    29401 gtatctgcac tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc
    29461 gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac
    29521 tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg
    29581 gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt
    29641 aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca
    29701 gggtgagggc aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac
    29761 cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt
    29821 atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg
    29881 cgaagtatgg aagtcaccca gcgaagcaaa ccaggccggt aacgctttct ccccatgacc
    29941 gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac
    30001 ggcatcaatg ccggactgta aacgatgatg cggcgtctgg tatcgcgcca gccagtattg
    30061 ccatgcacgc aaaaacgcat accccatcag cgtaacgggt ccccagattc cccccacaat
    30121 caaccagaac agcggagcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag
    30181 ctcacgcaaa tactcacgtt catcgcagcc tgccgggacg ccgtgaatca tggtgagttc
    30241 gccagccatc gtgacacggg catggctatc attacgtgaa gcagctgtca gataagcatg
    30301 ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag
    30361 cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc
    30421 catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa
    30481 gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa
    30541 aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcgcacga
    30601 aaccgcgccc aatcaaatgc agggccggga tcggttttcc gatccggcgc aatatcacaa
    30661 tggcccgtca tgttattagc gatatctgga tagcgatcaa tcagtgcccg cgtaaccgcc
    30721 gcaagctgtt gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc
    30781 ccaatagaaa aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat
    30841 gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga
    30901 gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt
    30961 ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg
    31021 tgcaccacca gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga
    31081 acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt
    31141 ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga
    31201 gttttaacat gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac
    31261 gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa
    31321 cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaactct cgctctcatg
    31381 ccacggtgat cacccgcgag aatggcgtat tttgcggcaa acgctgggtt gaagaggtgt
    31441 ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca
    31501 tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg
    31561 aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact
    31621 atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg
    31681 gcctgcgttc agctctgaaa tacgcggtac tttgcggcga cggagcgaat caccgtctgg
    31741 ggctttctga tgccttcctg atcaaagaaa atcatattat tgcctccggc tcagtgcgcc
    31801 aggccgtcga aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga
    31861 atctggaaga acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact
    31921 tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag
    31981 tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta
    32041 tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct
    32101 aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc
    32161 ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc
    32221 tgctttcccg tttcgttttt tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc
    32281 caaccaaatc aaggagcgaa acagatgg
//
LOCUS       DAJVTY010000026        32299 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVTY010000026 DAJVTY010000000
VERSION     DAJVTY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32299
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.026"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..1112
                     /gene="gspE"
                     /locus_tag="OQQ66_001120"
                     /pseudo
     CDS             1..1112
                     /gene="gspE"
                     /locus_tag="OQQ66_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1102..2304
                     /gene="hofC"
                     /locus_tag="OQQ66_001121"
     CDS             1102..2304
                     /gene="hofC"
                     /locus_tag="OQQ66_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1904126.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAGQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2339..3382)
                     /gene="guaC"
                     /locus_tag="OQQ66_001122"
     CDS             complement(2339..3382)
                     /gene="guaC"
                     /locus_tag="OQQ66_001122"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1904127.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(3538..3582)
                     /locus_tag="OQQ66_001123"
     CDS             complement(3538..3582)
                     /locus_tag="OQQ66_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904128.1"
                     /translation="MIIKTLKMSARKRN"
     gene            3607..4227
                     /gene="coaE"
                     /locus_tag="OQQ66_001124"
     CDS             3607..4227
                     /gene="coaE"
                     /locus_tag="OQQ66_001124"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1904129.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4227..4970
                     /gene="zapD"
                     /locus_tag="OQQ66_001125"
     CDS             4227..4970
                     /gene="zapD"
                     /locus_tag="OQQ66_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1904130.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            4980..5177
                     /gene="yacG"
                     /locus_tag="OQQ66_001126"
     CDS             4980..5177
                     /gene="yacG"
                     /locus_tag="OQQ66_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1904131.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5284..5673)
                     /gene="mutT"
                     /locus_tag="OQQ66_001127"
     CDS             complement(5284..5673)
                     /gene="mutT"
                     /locus_tag="OQQ66_001127"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1904132.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(5733..8438)
                     /gene="secA"
                     /locus_tag="OQQ66_001128"
     CDS             complement(5733..8438)
                     /gene="secA"
                     /locus_tag="OQQ66_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1904133.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(8500..9012)
                     /gene="secM"
                     /locus_tag="OQQ66_001129"
     CDS             complement(8500..9012)
                     /gene="secM"
                     /locus_tag="OQQ66_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1904134.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9243..10160)
                     /gene="lpxC"
                     /locus_tag="OQQ66_001130"
     CDS             complement(9243..10160)
                     /gene="lpxC"
                     /locus_tag="OQQ66_001130"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1904135.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLTFKAPSAVLA"
     gene            complement(10261..11412)
                     /gene="ftsZ"
                     /locus_tag="OQQ66_001131"
     CDS             complement(10261..11412)
                     /gene="ftsZ"
                     /locus_tag="OQQ66_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1904136.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(11473..12735)
                     /gene="ftsA"
                     /locus_tag="OQQ66_001132"
     CDS             complement(11473..12735)
                     /gene="ftsA"
                     /locus_tag="OQQ66_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1904137.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(12732..13562)
                     /gene="ftsQ"
                     /locus_tag="OQQ66_001133"
     CDS             complement(12732..13562)
                     /gene="ftsQ"
                     /locus_tag="OQQ66_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1904138.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(13564..14484)
                     /gene="ddlB"
                     /locus_tag="OQQ66_001134"
     CDS             complement(13564..14484)
                     /gene="ddlB"
                     /locus_tag="OQQ66_001134"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1904139.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(14477..15952)
                     /gene="murC"
                     /locus_tag="OQQ66_001135"
     CDS             complement(14477..15952)
                     /gene="murC"
                     /locus_tag="OQQ66_001135"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1904140.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16006..17073)
                     /gene="murG"
                     /locus_tag="OQQ66_001136"
     CDS             complement(16006..17073)
                     /gene="murG"
                     /locus_tag="OQQ66_001136"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1904141.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17070..18314)
                     /gene="ftsW"
                     /locus_tag="OQQ66_001137"
     CDS             complement(17070..18314)
                     /gene="ftsW"
                     /locus_tag="OQQ66_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1904142.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18314..19630)
                     /gene="murD"
                     /locus_tag="OQQ66_001138"
     CDS             complement(18314..19630)
                     /gene="murD"
                     /locus_tag="OQQ66_001138"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1904143.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(19633..20715)
                     /gene="mraY"
                     /locus_tag="OQQ66_001139"
     CDS             complement(19633..20715)
                     /gene="mraY"
                     /locus_tag="OQQ66_001139"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1904144.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(20709..22067)
                     /gene="murF"
                     /locus_tag="OQQ66_001140"
     CDS             complement(20709..22067)
                     /gene="murF"
                     /locus_tag="OQQ66_001140"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1904145.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22064..23551)
                     /gene="murE"
                     /locus_tag="OQQ66_001141"
     CDS             complement(22064..23551)
                     /gene="murE"
                     /locus_tag="OQQ66_001141"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1904146.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(23538..25304)
                     /gene="ftsI"
                     /locus_tag="OQQ66_001142"
     CDS             complement(23538..25304)
                     /gene="ftsI"
                     /locus_tag="OQQ66_001142"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1904147.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25320..25685)
                     /gene="ftsL"
                     /locus_tag="OQQ66_001143"
     CDS             complement(25320..25685)
                     /gene="ftsL"
                     /locus_tag="OQQ66_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1904148.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(25682..26623)
                     /gene="rsmH"
                     /locus_tag="OQQ66_001144"
     CDS             complement(25682..26623)
                     /gene="rsmH"
                     /locus_tag="OQQ66_001144"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1904149.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(26625..27083)
                     /gene="mraZ"
                     /locus_tag="OQQ66_001145"
     CDS             complement(26625..27083)
                     /gene="mraZ"
                     /locus_tag="OQQ66_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1904150.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(27685..28689)
                     /gene="cra"
                     /locus_tag="OQQ66_001146"
     CDS             complement(27685..28689)
                     /gene="cra"
                     /locus_tag="OQQ66_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1904151.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(28771..28857)
                     /locus_tag="OQQ66_001147"
     CDS             complement(28771..28857)
                     /locus_tag="OQQ66_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1904152.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(28869..29360)
                     /gene="ilvN"
                     /locus_tag="OQQ66_001148"
     CDS             complement(28869..29360)
                     /gene="ilvN"
                     /locus_tag="OQQ66_001148"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1904153.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29363..31087)
                     /gene="ilvI"
                     /locus_tag="OQQ66_001149"
     CDS             complement(29363..31087)
                     /gene="ilvI"
                     /locus_tag="OQQ66_001149"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1904154.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31206..31250
                     /locus_tag="OQQ66_001150"
     CDS             31206..31250
                     /locus_tag="OQQ66_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904155.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31405..>32299)
                     /gene="leuO"
                     /locus_tag="OQQ66_001151"
     CDS             complement(31405..>32299)
                     /gene="leuO"
                     /locus_tag="OQQ66_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1904156.1"
                     /translation="QLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLK
                     VMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVC
                     SPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFT
                     SVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQAS
                     IAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKQNSRTCYLSWHE
                     AAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7748 a         9080 c         7851 g         7620 t
ORIGIN      
        1 gttacaatct gcgctggaac aacgtgcgtc tgatattcat atcgaaccag cggacaatgc
       61 ctaccgcatc cgcttgcgta tcgacggcgt attgcatcct ttaccggatg tttcaccgga
      121 tgccggagtc gcattaaccg ccagattaaa agtgctggga aacctggata ttgcggaaca
      181 tcgcctgccg caggacgggc aattcactgt cgaactggca ggaaacgccg tctcatttcg
      241 tattgcgacc ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt tacagcaggt
      301 gaaccaggca ctggatgtta acacgctggg aatgcagccg ttacaactgg cggactttgc
      361 tcatgccttg caacaaccac agggactggt gctggtaact ggccctacag gcagcggcaa
      421 aacggtcacg ctttatagtg ccctgcaaaa gctgaatacc gctgacatta atatttgtag
      481 cgtcgaagat ccggttgaga tccccatagc cggactaaac cagacgcaaa tccatccgcg
      541 tgccgggctc acctttcagg gcgttttgcg tgcgttattg cgccaggatc ctgacgtcat
      601 catgatccga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc ggtcacctgg
      661 tgttgtctac cctacacact aattccacct gcgaaacgct ggtacgttta cagcaaatgg
      721 gagtcgcccg ctggatgctc tcatcagcgc ttacgctggt aatagcccag cgtctggtac
      781 gtaaattttg cccacattgt cgccagcagc aaggggagcc catccatatt ccagacaatg
      841 tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc taccacggtt
      901 tttatggtcg taccgccttg tttgaagtcc tgcccattac accagccata cgccagctta
      961 tttccgctaa taccgacgtt gaatcgctgg aaacgcacgc acgacaggcg ggtatgcgta
     1021 cgctttttga aaacggctgc ctagccgtgg agcaaggctt aaccaccttt gaagagttaa
     1081 tccgcgtact ggggatgccg catggcgagt aagcaactct ggcgctggca tggcataacc
     1141 ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtgcttt gctgctcatg
     1201 gcactacagc aacagatggt taccccactt agcctgaagc gaatcgccat caattctgcg
     1261 cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct actcaaagcc
     1321 gggttaacgc tttctgaagg gctggcactg ctggcgggac agcatcccag taagcaatgg
     1381 caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt ttccaatgcc
     1441 ttattaccct ggtcagaggt atttccgcca ctctatcagg cgatgatccg cacgggtgaa
     1501 ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa agcccagcgt
     1561 cagttgaccg acaaagtgaa atcagcgtta cgttatccca tcatcatttt agcgatggca
     1621 atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc tatctataag
     1681 accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc agactttagt
     1741 ggcgaatgga gctggctgct ggtgttgttc ggctttctgc tggcgatagc caataagttg
     1801 ctgatgcgcc gaccgacctg gcttatagtg cggcagaaat tgctgttacg catcccgatt
     1861 atgggttcac tgatgcgggg acaaaaactc acgcagatct ttacgattct ggcgctgaca
     1921 caaagtgcag gcattacttt tttgcagggc gtagagagcg tcagagaaac aatgcgctgc
     1981 ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg tcatcccatc
     2041 tggctggcgc taaaaaatac cggggagttt agcccgctct gtttgcaatt agtgagaaca
     2101 ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca tcatcgggat
     2161 aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt gctgatcata
     2221 acgggaggaa ttatcggtac gctggtggtg gcgatgtatc tgccaatttt ccatttaggc
     2281 gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacaccagcg ttggaagatt
     2341 acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg gataaatgtg gtgcgcttgg
     2401 tcagttcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg ccgcccaaaa
     2461 tatcacgcgc ggtattttca accgggcctc gcagcggcag cttaacggtt ttaccttctg
     2521 ctgcgcgata ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg gagctcatgc
     2581 cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg ctctcttcgt
     2641 ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag gcttttgcga
     2701 catcgcccgg cgtggtgcag ccaccgtcgc tgacgatcat tccgcccaga ccgtgcgccg
     2761 catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt ttgacgcgag
     2821 ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg gagaggataa
     2881 gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg gtcggccacg
     2941 cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc gccacatcaa
     3001 tacaaacaaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt tcgaaatccg
     3061 catcagacgt accggtagaa accatcacat gtttcagcac atcagcggaa gaattgttga
     3121 taaacgcttg ccactcttcg acagaatagt gtttatgcac agcagtcaaa atatcaaaag
     3181 aagccagcgc agaggccata gaaaatgtac ctacggtgtc catatttgcg gcgataatcg
     3241 gcacgccgga ccagctctga tctgaatgtt tgaaggtgaa ttgacgttcc agttcaacat
     3301 cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta aaacctaact
     3361 tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata agggtaagag
     3421 cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact gcgaaattgt
     3481 ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa cgtttcatca
     3541 atttcgcttt cgtgcactca tttttaacgt tttgataatc atgatgaatt cccgggaata
     3601 taaattatga ggtatatagt tgccttaacg ggaggcattg gcagtggcaa gagtaccgtt
     3661 gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat tgcgcgtcag
     3721 gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg cgctaacatg
     3781 attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt cgccaacccg
     3841 gaagagaaaa actggcttaa cgccctgctg catccgctga ttcagcaaga gacgcaacac
     3901 cagatccagc aagctacttc cccctatgta ctgtgggttg tgccattgct ggtagaaaac
     3961 tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga aacgcaactt
     4021 aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg tcgaacaaat ccttgctgct
     4081 caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa taacggcgca
     4141 ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca gcttgcgtcg
     4201 cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa catccactaa
     4261 atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc accgttaatt
     4321 tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt gaattactgg
     4381 atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttgac cggcagcaac
     4441 gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt gaagcattaa
     4501 ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc gggcaatttc
     4561 tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca ggcggctgtt
     4621 gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg cagcgcgaca
     4681 gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt accatggtgc
     4741 tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat ggtttttatc
     4801 aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat tcacagcttt
     4861 atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg ctggacagtg
     4921 aaaacggaca ggtaccggaa cgtctagatt tcgaactggc ctgttgctaa ggagtgaaaa
     4981 tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggta tggggtgaaa
     5041 tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc ggagaatggg
     5101 ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat gactggagcg
     5161 aagagccaaa gcagtgacat tggtctgatg ccgcacatag gcctgataag acgcggacag
     5221 cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta tccgacctac
     5281 agactacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc
     5341 attaagaccg accagcgaca tccactcacc gggttgccct tctttacccc acggcacccc
     5401 ttcccaactt tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatatgc
     5461 cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acctcttcct gaagttcacg
     5521 caccaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa actccagttt
     5581 attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg
     5641 aataatacct accgcaattt gcagcttttt cattgtttta aacctataaa aaaggcgcag
     5701 aatcctgcgc cttttgcttc aacagttagc ttttattgca ggcggccatg acactgcttg
     5761 tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctcaccggtt
     5821 tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt
     5881 tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc
     5941 atacgtacct gaactttgct cagcgtactg ataacttcat atttcaacga ctccagcatc
     6001 gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag
     6061 ccacgcaggt ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg
     6121 gagtcaagcg tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc
     6181 acttcttctt tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc
     6241 tcttcatgca gttctggttc tttatccagc cactcggcaa ttggtaaatc gaggtcgaaa
     6301 tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc
     6361 ggaatgtagg catcaatggt ggctttgaac acatcttcac gaatgctgtt gatggtttcg
     6421 ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg
     6481 ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact
     6541 ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc
     6601 ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc
     6661 gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga
     6721 ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg
     6781 ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt
     6841 tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca
     6901 atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg ataacctgcc
     6961 tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt gtgcttaata
     7021 ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc
     7081 agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt
     7141 tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc
     7201 ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc ggtatcagca
     7261 gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa
     7321 gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc
     7381 agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata
     7441 acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg agcgcgcagc
     7501 gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca
     7561 tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg
     7621 ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag
     7681 tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc
     7741 gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg
     7801 gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg
     7861 aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg
     7921 tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccgaca
     7981 gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag
     8041 tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc
     8101 gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga
     8161 accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc
     8221 tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga
     8281 cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc
     8341 ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta
     8401 cgactaccga aaactttagt taacaatttg attagcataa taaaatctca aacgccccgc
     8461 gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc gttgagggcc
     8521 agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt
     8581 aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag
     8641 cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc
     8701 aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg tgcgaatggc
     8761 atattgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt tcgcttccag
     8821 caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt
     8881 tgcgggcacg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac
     8941 catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag
     9001 tattccactc acgttattgc catcccgatc gaattcaaac gtatttagcg cacaaatctt
     9061 atcattaaag cctgaagtcc acagcatgaa tgaagataag ttgtgcgtaa aagtgcttaa
     9121 gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt
     9181 tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt
     9241 cgttatgcca gtacagctga aggcgctttg aaggtcaacg gcagttctgc gtcgtcctgg
     9301 aaggtcacat attcccaagc ttcctgtttc gccaggacag cctgcagcag tttgttattc
     9361 agtgcatgac cggatttata agcgataaat gcaccaataa tattgtgacc acacatgaac
     9421 aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg
     9481 tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg
     9541 cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg
     9601 ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa
     9661 tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta
     9721 aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt
     9781 ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg
     9841 ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc
     9901 aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg
     9961 accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc
    10021 ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac
    10081 gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta
    10141 agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac
    10201 tatacattcg gcgggccagt ttagcacaaa gagcctcgaa accaaaattc cagtcaattc
    10261 ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt
    10321 ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc
    10381 catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag
    10441 agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta cgcgcagctc
    10501 gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa
    10561 tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt
    10621 gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca gcggagaaga
    10681 gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat
    10741 cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa
    10801 acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta catcgttcgc
    10861 tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt
    10921 cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa
    10981 tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat
    11041 acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc
    11101 catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca atgcatcgcg
    11161 atcctcatca gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt
    11221 gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc
    11281 ggtatttacc gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc
    11341 attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat
    11401 tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg
    11461 gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc
    11521 aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc
    11581 atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc
    11641 ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc
    11701 tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg
    11761 tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt
    11821 gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg
    11881 tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc
    11941 cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc
    12001 gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa
    12061 tgccccaccg gtataaacgg cgatatccat tgtaccacct ccgatatcga cgacgcagac
    12121 acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat
    12181 cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc
    12241 gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg
    12301 attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg
    12361 atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg
    12421 cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt
    12481 accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc
    12541 aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat
    12601 accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc
    12661 ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc
    12721 cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct
    12781 ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta
    12841 atccgtttgc catcggtttg cgccagctgc tgtaaaaccg gataaagttc tacaaagcga
    12901 gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc
    12961 aactgccagg aacgccgcgc ggtcatcgcc gtttccttca gagtaaatct gtcctttgcc
    13021 agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca
    13081 tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa ggtgtttcct
    13141 tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga
    13201 atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt
    13261 tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc
    13321 aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac
    13381 accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg
    13441 ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca
    13501 ttattgcggc gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac
    13561 atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg
    13621 acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag
    13681 cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc
    13741 taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcatccag
    13801 acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa
    13861 ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt
    13921 gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa
    13981 tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca cactggaacc
    14041 ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt
    14101 atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg cgaccggtaa
    14161 accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg cagatgccat
    14221 cactccgctg ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtaccatc
    14281 ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg acttcagttg
    14341 cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccacctt cacgcagccc
    14401 ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag tcccgcccaa
    14461 caggaccgcg attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga
    14521 tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt
    14581 cgttaccggt taataccggt gccagcatct cggctacctg cgcagggtcc ggcaccagaa
    14641 tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg
    14701 gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca
    14761 gcacattggc gaaatcatca tacaagtcgc gcgtacgggt aaaacggtgc ggctgaaaca
    14821 gcattaccag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg gcgtcaactt
    14881 ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg
    14941 gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtccccagg aagctctcca
    15001 gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca accgcagctg
    15061 cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc ggctctttgt
    15121 cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca
    15181 cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaacaatt
    15241 cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca
    15301 gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt
    15361 cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac
    15421 tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg
    15481 cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg
    15541 aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg ccatgacgaa
    15601 aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag
    15661 ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc acgctggcat
    15721 cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc attaactgct
    15781 gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa
    15841 cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc
    15901 gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg
    15961 ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc
    16021 ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg
    16081 ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc
    16141 cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc
    16201 attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc
    16261 cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat ccgcccacgc
    16321 atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc
    16381 ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc agatagtgac
    16441 tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg
    16501 ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg
    16561 caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa
    16621 agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa
    16681 gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag
    16741 accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt tgtacgcttt
    16801 cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag ctatcagtgc
    16861 ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt
    16921 tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg
    16981 agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc
    17041 catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct
    17101 ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc
    17161 agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc
    17221 cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag
    17281 gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat cgacatcgcg
    17341 cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct
    17401 tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt
    17461 accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat cagcgattgc
    17521 gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg
    17581 cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc cataccgata
    17641 atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca
    17701 aacaccacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga
    17761 atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc
    17821 accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc aggctggata
    17881 cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctccc cactaccagc
    17941 acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac
    18001 tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca
    18061 tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc
    18121 gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag taaggtgcga
    18181 tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccttttagc
    18241 gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg
    18301 agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt
    18361 caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc atatcgcccg
    18421 gctgaacacg cggagccagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca
    18481 cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac aaacgcacgt
    18541 tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca tcgccgccca
    18601 gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc gtactgccga
    18661 cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc accacttcaa
    18721 agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg cgcggcaaac
    18781 cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc tgcccggaaa
    18841 gtttcatctc tttcacgttc agcactttct cgcctttcac tcgcagccag gtttctccct
    18901 gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatcag
    18961 caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact ttcgcatttt
    19021 cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag cggtccatat
    19081 gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa gtggtttcca
    19141 gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc agcatcaacg
    19201 ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac
    19261 ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt
    19321 gtgcttcgcg gcagaatagc tcgatatcgc caacgatttc gattccggca tcagcggcag
    19381 cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca
    19441 tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca
    19501 ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa
    19561 agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctgat
    19621 aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca gcatcagcga
    19681 aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg
    19741 atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag
    19801 gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg
    19861 acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac ctacatcgcc
    19921 cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg ccccgactat
    19981 cgcggtacag acaataacca gttccccggc gtgtcgcaga tacggtatgt gtaaatagct
    20041 ggcaaagttc atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac
    20101 ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt tgccagtacc
    20161 cacaatgacg aagtaagcca gcagaatgta gaacagcccc aactgcggca tcacatcttt
    20221 aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagggcgaa
    20281 ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt
    20341 ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac
    20401 cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga
    20461 gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa
    20521 gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc
    20581 aatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc
    20641 gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc
    20701 cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca
    20761 ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga
    20821 cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc ggtgctgata
    20881 gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg
    20941 cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc
    21001 agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat tgaaccgaca
    21061 ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga
    21121 cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc
    21181 acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac
    21241 ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt accgtgcgag
    21301 gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac
    21361 actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata
    21421 atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc
    21481 gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgcagcttcc
    21541 gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc aagttcaata
    21601 actgcgtaat cgtattccgg cgttaagcgc aacaacgtca tcggtacacc gatgtcgttg
    21661 ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc
    21721 tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc cggaacttgc
    21781 tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc
    21841 ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc agctttcgcc
    21901 tggtcggcaa aatcgtgggc atcaaaacgt tcgcctttca gggcaacaaa caggcagccc
    21961 ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt
    22021 tcaccattga gaatgtcggt aagttggcta agggttacgc taatcatgca atcaccccca
    22081 gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt
    22141 aatcttcatg gcctttgccc gcgaccagca ccacatcgtt ctctttagcc tgcataacgg
    22201 cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc
    22261 ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca
    22321 ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat
    22381 cgcgatcgcc accacagcca aagacacacc acagcttgcc cgcacagtgc agacgcgccg
    22441 cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt
    22501 tgcctggcac agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt
    22561 tcaacagatc agccaatgga tagccgagtg ccaacagtgt cgccagcgcg agcagcaggt
    22621 tgctgacgtt aaaagcgcct atcagatggc tttcaatttc gccatcgccc caacttgagc
    22681 taaagcgaat cgtcacaccg ctgtcgtgat agttcacgtc gatcactttt aaccagcgtc
    22741 cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc ggcagctttg
    22801 ccagccaacg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgtt
    22861 cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga taatcaaggt
    22921 gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct
    22981 gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc tccaccagcc
    23041 ccgccagctc atgctgaaca tcgactgccg aaccggtggt attttctgtc gggatcactt
    23101 tccccagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc
    23161 tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt acgcctacga
    23221 gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt
    23281 tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg
    23341 cctcatcttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat
    23401 atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca
    23461 cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag
    23521 caagaaggtc gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat
    23581 tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg
    23641 cccatgatgg caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc
    23701 ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg
    23761 gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg
    23821 gttttaatgg cgatacgata gcctttaatc gccgccttca cgccgccgcc gcccggtagc
    23881 gccacgcttt ccatcatatg caccacggtg cggacaatgg attccgggaa gacacgttca
    23941 ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg
    24001 atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag
    24061 aaggtggccc tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct
    24121 ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac
    24181 gctgaggacg gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat
    24241 accccggtca gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt
    24301 cgataaggaa tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc
    24361 atcaccacca tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta
    24421 cgcatcgcct ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc
    24481 atcgccagca cttcacctgt gttgacatcc accagcacgg cgctaccaga ttcagccttg
    24541 ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata
    24601 ctcagcgcca ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga
    24661 ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa
    24721 ctcttctcaa cgccctcaat cccttgacta tcgacgttag taaagccaat gaggtgagca
    24781 gtcacttcgc cggatggata ataacggcga gattcttcac gcagatgaat acccggcagt
    24841 ttcagttttt tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag
    24901 cgccctttcg ggttggcgtt aatgcgggca gaaagctgat ccagcggaat attgagcgcg
    24961 ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc
    25021 gggtcagccc aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca
    25081 gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct
    25141 ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc
    25201 agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactaataaa gttggcatgt
    25261 tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct
    25321 tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc
    25381 gtggcgatcc tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg
    25441 cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt
    25501 aaacgggtat ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac
    25561 aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca
    25621 tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg
    25681 atcatgcgtt cgtcctctct gcgatacgca gaactgaact acgggcacga ggattctcag
    25741 ccacctcttc ttcgcccggc attaacttgc ctaatgctcg cagctgacgg ccacccagtt
    25801 ttttgagctg ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt
    25861 cacgcataaa acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc
    25921 gcccacccgg ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt
    25981 cactgtttac ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat
    26041 ctttcaccgg cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct
    26101 gttcgcggtt acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat
    26161 aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg
    26221 actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc
    26281 cacgttcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga
    26341 tcttgccgat aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga
    26401 tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat
    26461 cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac
    26521 cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg
    26581 cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc
    26641 tgcagtcgct ccgataagtc tccagtagcc aactgctctg cgtcgatatc ttccttgacc
    26701 tgttgatgcc aagttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc
    26761 acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg
    26821 ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaact
    26881 gggttcatgc tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg
    26941 taaagcagca ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc
    27001 tcaagcagct gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg
    27061 actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat
    27121 ttcccactga aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat
    27181 accgaggtaa aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac
    27241 gcgcgaagca aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcactg
    27301 ttttaaaatg actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg
    27361 gcattttagg acatatcttc cccggttaac agtccttgtt acgtctgtgt ggcgcttgat
    27421 gccagcgggt ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatcgccgc
    27481 atgtaacaaa ataatgcaaa aacgcgctgt cgatatcact taattattgt gaatttcagc
    27541 tggtgagtta attaacgggc gggtttaaaa ccatttatca ataatagtca ggaatagtct
    27601 tatttacttt aagcatattg atgtccagtc ccgtactcta cgcgccagag ggaaattcac
    27661 ctggcgcgta tttttgttcg cggcttagct acggctgagc acaccgcggc gatagagatt
    27721 acgtttaatg cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac
    27781 aatctccagc acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca
    27841 ctgtaagaag tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag
    27901 tttaccatcg cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt
    27961 gaacagcgcc tgcggcatca gatgcgtttc cagccatttt tcgaataact gggcagccgc
    28021 ctcccgctca tagctgttgg catacaggaa atgcacttcg cgcggatcat ctttccaggc
    28081 agtacggaaa ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata
    28141 aagcaccgtc tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg
    28201 atcggcacca accacgctgg tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat
    28261 cgggaacggg gcgttagccc agcgttgata aaaaggatgc tcaggtggca acgacgtcga
    28321 aacaataatg gcatcaacct gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc
    28381 tggctgatct tctgagcagg caatcagcag ttgataaccc cgttgccgcg cctggcgctc
    28441 aagatagtta gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat
    28501 agaacgtgtg cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc
    28561 acgcaccaca gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt
    28621 gccgttaata acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc
    28681 cagtttcaca attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg
    28741 ttaaattcat taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt
    28801 tcccactgat ttcgcaaaaa aagcccagcg tgacatgttg ggctgcaaat tgcacactga
    28861 gatcattctc aacgcattat tttatcgccg cgcgaaagtc cgaccacacc agagcgagca
    28921 acttccacaa ttttcgccac ctcgcgaatc gatgctaaaa atgcatcaag cttatcgctg
    28981 gtgcctgcta attgaacggt ataaagcgag ggggtgacat cgataatttg cccacggaat
    29041 atttccgtat tacgtttcac ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc
    29101 ataatttccc gctcaacatg cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc
    29161 agcttgtgta attgcttttc gatctgctca agtacttttt catcgcccac ggtctggatg
    29221 gtcatacgcg ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag
    29281 cctcgctggg aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag
    29341 actgataata tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca
    29401 ttccgccccc gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtaacatcaa
    29461 caaacaccag gcgattattg cgcacctgtt ccagcgcctc gctaagtttg ctttccagct
    29521 catgcggatg agagatctgg atcccgacgt gaccataggc ttccgccaga cggacgaaat
    29581 cgggtagtga ttgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact
    29641 gtttcaccat ccccagatag cggttattga gattcaccac cagtacgggc aactcgtatt
    29701 gcaacgcggt agacagttcc tgaatgttca tctgaatact gccgtcgcca gtgacgcaaa
    29761 ccacggtttc ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca
    29821 tcgtgccgag gccaccggaa ttgatccagc gacgcggttt gtcgaatgga taataaagtg
    29881 cagcaaacat ctggtgctgc ccgacatcgg acgtcacgta agcgtctccc ttcgtcaacc
    29941 gccaaagagt ctcgatcacc gcctgcggtt taatcttttc actgtgagtg tcatatttca
    30001 ggcactgacg agcgcgccac tgttcaattt gctgccacca gtcgcggatc tcatccagtg
    30061 gttgatgggc ggattcttgc gacaagagtt caagcatttg ttcgaggacc tggcgagcat
    30121 cccccacaat cgggatatcc gcagtcacgg ttttagaaat ggaagtagga tcaatatcga
    30181 tatgcagaac agtggcattt gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa
    30241 atcgtacccc gacggcgaaa atcacatccg cgttatgcat cgtcatattg gcttcgtagg
    30301 taccgtgcat tcccagcatg cccagtgcct gacgatgcgt tgccggaaac gcccccagcc
    30361 ccatcaatga ggaaacaacg ggcagattca acgcctccac cgtttctttc aactgctgat
    30421 ggcagcccgc catgattgcc ccaccgccta cgtagacaac cggttttttt gccactacca
    30481 gcgtttgcag agcacgctta atttgccctt tatgtccggt agtagtggga ttgtaagaac
    30541 gcatactgac cgactccggc cagacatagg gtaatttgtt cgccggatta agaatatctt
    30601 tcggtaaatc aacgactact ggtccaggac gaccacttgc cgccagccag aaagcctttt
    30661 tcagcacctg cggaatgtct tccgtttgct taaccagaaa actgtgttta accactggtc
    30721 gcgaaatccc caccatgtcg cactcctgaa aggcatcgta acctatcaac gaggtcgcta
    30781 cctgcccgga aaggacaact aatggaatgg aatccatata agcggtggcg atgccggtaa
    30841 tcgcattggt cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc
    30901 gcgccagacc atcggccata tgcactgccg cctgctcatg acgaactaaa acatgatcga
    30961 taccacccac ggtatgcaat gcatcgtaaa tatcaaggac tgcgcctcca ggataaccga
    31021 atacttgttt aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca
    31081 tctccatggc ctgcctcact gtttgatgga aaaaatgtgt aaaacacatt aaccgtccga
    31141 tcttgcttaa gcaattggca gccagagaca taaacgcagg gtaataagaa taaacaggag
    31201 gaaagatgaa aagaaacgat aaaatcctcc attacgaagg attaaattga taataaatcg
    31261 gctgaatccc acaacttaca gcaatttctc tattttttta attaatccta aaatagagag
    31321 cccacacatt cagaataaaa ttctacattc aataaaaaat gcagaataaa ccagacattc
    31381 atgtctgacc tattctgcat tcagttagcg tttgcaaatt gagactaatt gttcttccat
    31441 ccactgatgg cctttatcgc gcccggcagc ttcatgccat gagagataac aggttctgct
    31501 gttttgtttt aacggcagcg gtaatacctg taattctaag gattcagcga actcttcagc
    31561 cagccaacgc ggcgcaatag cgaccaaatg cgtttgcgac accacgctaa gtacgctcat
    31621 cattgccatg ccctgatacg cgatactggc ttgcttatct accgtgtcat accaaggttg
    31681 actaaatgac gcgaaacgat cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg
    31741 tttcagtaac gggcctttaa ttgttggatg atttttgctg gctaccagca ccatttcatc
    31801 tttaaataat ggcacgctgg taaattcagg acgatggaag tcttcataac taatcacaaa
    31861 ctccgtttcc tgataacgca gctgatgttc agtgttctga tttaatgaag acttgaacat
    31921 aacatgtata tttggcgcaa tctgctcaat gtgattataa atctgcgagg tcagaatgct
    31981 gtctaacggg ctgcaaacac aaagatgaaa tacacgttca ctactcgcgg gttcaaaacc
    32041 tgaaccaggc aattcatttt gtactagttg caatgcctgg cgaactgaac caaaaagttg
    32101 aaatgcgcga gcagtcggtt gaataccacg gccataacga acaaaaagct cgtcattaaa
    32161 catcaccttc aggcgtgcaa cagcgttact gaccgcaggt tgcgacatac ccagaacatg
    32221 agcggcacgg gtaatgtttt gctcctgcat cacggcatcg aaaacggtta ataagttgag
    32281 atcgaccatg cgtagctgt
//
LOCUS       DAJVTY010000027        31877 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVTY010000027 DAJVTY010000000
VERSION     DAJVTY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31877
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.027"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="OQQ66_001152"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="OQQ66_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1904157.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1184..1567
                     /gene="secE"
                     /locus_tag="OQQ66_001153"
     CDS             1184..1567
                     /gene="secE"
                     /locus_tag="OQQ66_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1904158.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1569..2114
                     /gene="nusG"
                     /locus_tag="OQQ66_001154"
     CDS             1569..2114
                     /gene="nusG"
                     /locus_tag="OQQ66_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1904159.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2274..2702
                     /gene="rplK"
                     /locus_tag="OQQ66_001155"
     CDS             2274..2702
                     /gene="rplK"
                     /locus_tag="OQQ66_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1904160.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2706..3410
                     /gene="rplA"
                     /locus_tag="OQQ66_001156"
     CDS             2706..3410
                     /gene="rplA"
                     /locus_tag="OQQ66_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1904161.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3823..4320
                     /gene="rplJ"
                     /locus_tag="OQQ66_001157"
     CDS             3823..4320
                     /gene="rplJ"
                     /locus_tag="OQQ66_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1904162.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4387..4752
                     /gene="rplL"
                     /locus_tag="OQQ66_001158"
     CDS             4387..4752
                     /gene="rplL"
                     /locus_tag="OQQ66_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1904163.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            5072..9100
                     /gene="rpoB"
                     /locus_tag="OQQ66_001159"
     CDS             5072..9100
                     /gene="rpoB"
                     /locus_tag="OQQ66_001159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1904164.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9177..13400
                     /gene="rpoC"
                     /locus_tag="OQQ66_001160"
     CDS             9177..13400
                     /gene="rpoC"
                     /locus_tag="OQQ66_001160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1904165.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQML
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13613..14152
                     /locus_tag="OQQ66_001161"
     CDS             13613..14152
                     /locus_tag="OQQ66_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCS1904166.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14562..15695)
                     /gene="thiH"
                     /locus_tag="OQQ66_001162"
     CDS             complement(14562..15695)
                     /gene="thiH"
                     /locus_tag="OQQ66_001162"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1904167.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15692..16462)
                     /gene="thiG"
                     /locus_tag="OQQ66_001163"
     CDS             complement(15692..16462)
                     /gene="thiG"
                     /locus_tag="OQQ66_001163"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1904168.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEEIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16464..16664)
                     /gene="thiS"
                     /locus_tag="OQQ66_001164"
     CDS             complement(16464..16664)
                     /gene="thiS"
                     /locus_tag="OQQ66_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1904169.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHILQDGDQILLFQVIAGG"
     gene            complement(16648..17403)
                     /gene="thiF"
                     /locus_tag="OQQ66_001165"
                     /pseudo
     CDS             complement(16648..17403)
                     /gene="thiF"
                     /locus_tag="OQQ66_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(17396..18031)
                     /gene="thiE"
                     /locus_tag="OQQ66_001166"
     CDS             complement(17396..18031)
                     /gene="thiE"
                     /locus_tag="OQQ66_001166"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1904170.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(18031..19926)
                     /gene="thiC"
                     /locus_tag="OQQ66_001167"
     CDS             complement(18031..19926)
                     /gene="thiC"
                     /locus_tag="OQQ66_001167"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1904171.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(20007..20107)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20160..20636)
                     /gene="rsd"
                     /locus_tag="OQQ66_001168"
     CDS             complement(20160..20636)
                     /gene="rsd"
                     /locus_tag="OQQ66_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1904172.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20731..21504
                     /gene="nudC"
                     /locus_tag="OQQ66_001169"
     CDS             20731..21504
                     /gene="nudC"
                     /locus_tag="OQQ66_001169"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1904173.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21544..22608
                     /gene="hemE"
                     /locus_tag="OQQ66_001170"
     CDS             21544..22608
                     /gene="hemE"
                     /locus_tag="OQQ66_001170"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1904174.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22618..23289
                     /gene="nfi"
                     /locus_tag="OQQ66_001171"
     CDS             22618..23289
                     /gene="nfi"
                     /locus_tag="OQQ66_001171"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1904175.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            23332..23922
                     /locus_tag="OQQ66_001172"
     CDS             23332..23922
                     /locus_tag="OQQ66_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1904176.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            24109..24381
                     /gene="hupA"
                     /locus_tag="OQQ66_001173"
     CDS             24109..24381
                     /gene="hupA"
                     /locus_tag="OQQ66_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1904177.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24394..25089
                     /locus_tag="OQQ66_001174"
     CDS             24394..25089
                     /locus_tag="OQQ66_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1904178.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(25091..25516)
                     /gene="zraP"
                     /locus_tag="OQQ66_001175"
     CDS             complement(25091..25516)
                     /gene="zraP"
                     /locus_tag="OQQ66_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1904179.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25754..27130
                     /gene="zraS"
                     /locus_tag="OQQ66_001176"
     CDS             25754..27130
                     /gene="zraS"
                     /locus_tag="OQQ66_001176"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1904180.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            27127..28451
                     /gene="zraR"
                     /locus_tag="OQQ66_001177"
                     /pseudo
     CDS             27127..28451
                     /gene="zraR"
                     /locus_tag="OQQ66_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(28448..29737)
                     /gene="purD"
                     /locus_tag="OQQ66_001178"
     CDS             complement(28448..29737)
                     /gene="purD"
                     /locus_tag="OQQ66_001178"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1904181.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29749..31338)
                     /gene="purH"
                     /locus_tag="OQQ66_001179"
     CDS             complement(29749..31338)
                     /gene="purH"
                     /locus_tag="OQQ66_001179"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1904182.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7551 a         8219 c         8706 g         7401 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      961 ataacatttg actcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
     1021 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
     1081 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
     1141 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
     1201 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
     1261 cttggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
     1321 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
     1381 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
     1441 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
     1501 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
     1561 gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
     1621 ttgaaggccg cgtagcaacg tcgctgcgtg agcatatcaa attacacaac atggaagatt
     1681 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1741 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1801 cgagctggca cttggtgcgc agcgtaccgc gagtgatggg cttcatcggc ggtacttccg
     1861 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1921 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1981 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     2041 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     2101 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgcga
     2161 gattggaata caatttcgcg ccttttattt ttatgggcct tgcccgtaaa aacgattttt
     2221 tatatcacgg ggagcctctc agaggcgtta ttacccaact tgaggaattt ataatggcta
     2281 agaaagtaca agcctatgtc aagctgcagg ttgcagctgg tatggctaac ccgagtccgc
     2341 cagtaggtcc ggctctgggt cagcagggcg taaacatcat ggaattctgc aaagcgttca
     2401 acgcaaaaac tgattccatc gaaaaaggtc tgccgattcc ggtagtaatc accgtttacg
     2461 ctgaccgttc tttcactttc gttaccaaga ccccgccggc agcagttctg ctgaaaaaag
     2521 cggctggtat caagtctggt gccggtaagc cgaacaaaga caaagtgggt aaaatttccc
     2581 gcgctcagct gcaggaaatc gcgcagacca aagctgccga catgactggt gccgacattg
     2641 aagcgatgac tcgctccatc gaaggtactg cacgttccat gggcctggta gtggaggact
     2701 aagaaatggc taaactgacc aagcgcatgc gtgttatccg cgagaaagtt gatgcaacca
     2761 aacagtacga catcaacgaa gctatcgcac tgctgaaaga gctggcgact gctaaattcg
     2821 tagaaagcgt ggacgtagct gttaacctcg gcatcgacgc tcgtaaatct gaccagaacg
     2881 tacgtggtgc aactgtactg ccgcacggta ctggccgttc cgttcgcgta gccgtattta
     2941 cccaaggtgc aaacgctgaa gctgctaaag ctgcaggcgc agaactggta ggtatggaag
     3001 atctggctga ccagatcaag aaaggcgaaa tgaactttga cgttgttatt gcttctccgg
     3061 atgcaatgcg cgttgttggc cagctgggcc aggttctggg tccgcgcggc ctgatgccaa
     3121 acccgaaagt gggtactgta acaccgaacg ttgctgaagc ggttaaaaac gctaaagctg
     3181 gccaggttcg ttaccgtaac gacaaaaacg gcatcatcca caccaccatc ggtaaagtgg
     3241 actttgacgc tgacaaactg aaagaaaacc tggaagctct gctggttgcg ctgaaaaaag
     3301 caaaaccgac tcaggcgaaa ggcgtgtaca tcaagaaagt tagcatctcc accaccatgg
     3361 gtgcaggtgt tgcagttgac caggctggcc tgagcgcttc tgtaaactaa tgcctttacg
     3421 tgggcggtga ttttgtctac aatcttaccc ccacgtataa tgcttaatgc agacgtatat
     3481 ccgagatatt cgggttgtgg caaggcggca actgagtgag tcgccaagag catagctaac
     3541 tatgtgactg gtgcgaatga aggaagccaa cgccgccaca agttgaatag cgacggatag
     3601 aaaagatttg ttcgttggag cttggcctat ccaggcctcc gtcgaagacc gcaggagttt
     3661 cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat tcttttatat
     3721 tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg agtgaagtga
     3781 gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt aaatcttcaa
     3841 gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct gtctgcagta
     3901 gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa agcaggtcgc
     3961 gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc tgttgaaggt
     4021 actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc atactctatg
     4081 gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa tgcaaaattt
     4141 gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat cgaccgcctg
     4201 gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat gaaagaagct
     4261 tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga agctgcttaa
     4321 tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata ttcaggaaca
     4381 atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat gtctgtaatg
     4441 gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc tgctgctgct
     4501 gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt cgacgtaatt
     4561 ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg cgcaactggc
     4621 ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct gaaagaaggc
     4681 gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg cgctgaagtt
     4741 gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga tggctggtga
     4801 ctttttagtc accagccttt ttgcgctgta aggcgccagt agcgtttcac actgtttgac
     4861 tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc gacaggacgt
     4921 ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc attgcggaaa
     4981 gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca atggacagat
     5041 gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg agaaaaaacg
     5101 tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc tcctttctat
     5161 ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt atggtctgga
     5221 agcagctttc cgttccgtat tcccgattca gagctacagc ggtaattccg agctgcaata
     5281 cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa tccgtggcgt
     5341 gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc gcgaagcgcc
     5401 ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa ttccgctcat
     5461 gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt cccagctgca
     5521 ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt cgggtaaagt
     5581 gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg aattcgatcc
     5641 gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga ccatcattct
     5701 gcgcgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa aagttatctt
     5761 tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc gtggtgaaac
     5821 cgcatctttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc gccgtatcac
     5881 tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa cttatcgaag tcccggttga
     5941 gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg gcgagctgat
     6001 ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc agtctggtca
     6061 caagcgtatc gaaacgctgt tcaccaacga tctggatcac ggcccatata tctctgaaac
     6121 cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct accgcatgat
     6181 gcgccctggc gagccgccga ctcgtgaagc agctgaaagc ctgttcgaga acctgttctt
     6241 ctccgaagac cgttatgact tgtctgcggt tggtcgtatg aagttcaacc gttctctgct
     6301 gcgcgaagaa atcgaaggtt ccggtatcct gagcaaagac gacatcattg atgttatgaa
     6361 aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc acctcggcaa
     6421 ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg gcttggtacg
     6481 tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc tgatgcctca
     6541 ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg gttccagcca
     6601 gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca aacgtcgtat
     6661 ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag ttcgagacgt
     6721 acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc cgaacatcgg
     6781 tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc ttgagactcc
     6841 gtatcgtaaa gtgaccgacg gtgttgtaac tgacgaaatt cactacctgt ctgctatcga
     6901 agaaggcaac tacgttatcg cccaggcgaa ctccaacttg gatgaagaag gccacttcgt
     6961 agaagacctg gtaacttgcc gtagcaaagg cgaatccagc ttgttcagcc gcgaccaggt
     7021 tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc tgatcccgtt
     7081 cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac gtcaggccgt
     7141 tccgactctg cgcgctgata agccgctggt tggtactggt atggaacgtg ctgttgccgt
     7201 tgactccggt gtaactgcgg tagctaaacg tggtggtgtc gttcagtacg tggatgcttc
     7261 ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag gtatcgacat
     7321 ctacaacctg accaaataca cccgttctaa ccagaacacc tgtatcaacc agatgccgtg
     7381 tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc cgtccaccga
     7441 cctcggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt ggaatggtta
     7501 caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc gtttcaccac
     7561 catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc cggaagagat
     7621 caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactagatg aatccggtat
     7681 cgtttacatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg taacgccgaa
     7741 aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg gtgagaaagc
     7801 ctctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta cggttatcga
     7861 cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg aaatcgaaga
     7921 aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc tcgaagcggg
     7981 tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg agaagctcga
     8041 caaactgccg cgcgatcgct ggctggagct gggcctgaca gacgaagaga aacaaaatca
     8101 gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga agaaactcga
     8161 agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc tgaagattgt
     8221 taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg caggtcgtca
     8281 cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt acgatgaaaa
     8341 cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta tgaacatcgg
     8401 tcagatcctc gaaacccacc tgggtatggc tgcgaaaggt atcggcgaca agatcaacgc
     8461 catgctgaaa cagcagcaag aagtcgcgaa actgcgcgaa ttcatccagc gtgcgtacga
     8521 tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg aagaagttat
     8581 gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt tcgacggtgc
     8641 gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt ccggtcagat
     8701 ccgcctgtac gatggtcgca ctggtgaaca gttcgagcgt ccggtaaccg ttggttacat
     8761 gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt ccaccggttc
     8821 ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg gtcagcgttt
     8881 cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc tgcaggaaat
     8941 gctcaccgtt aagtctgatg acgtgaacgg tcgtaccaag atgtataaaa acatcgtgga
     9001 cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt tgaaagagat
     9061 tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca aacaggtcac
     9121 tgctgtcggg ttaaaacccg gcagcggatt gtgctaactc cgacgggagc aaatccgtga
     9181 aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat gcgatcaaaa
     9241 ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt aaaaagccgg
     9301 aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc gcccgtatct
     9361 ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg aaacaccgtg
     9421 gcgtcatctg tgagaagtgc ggcgttgaag tgacccagac taaagtacgc cgtgagcgta
     9481 tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa tcgctgccgt
     9541 cccgtatcgg tctgctgctc gatatgccgc tgcgcgatat cgaacgcgta ctgtactttg
     9601 aatcctatgt ggttatcgaa ggcggtatga ccaacctgga acgtcagcag atgctgactg
     9661 aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg aagatgggtg
     9721 cggaagcaat ccaggctctg ctgaagagca tggatctgga gcaagagtgc gaacagctgc
     9781 gcgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc aagcgtatca
     9841 aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc ctgaccgttc
     9901 tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt cgtttcgcga
     9961 cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt ctgaaacgtc
    10021 tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg ctgcaggaag
    10081 cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt tctaacaagc
    10141 gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc cgtcagaacc
    10201 tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtaggt ccatacctgc
    10261 gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa ccgttcatct
    10321 acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag aaaatggttg
    10381 agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa cacccggtac
    10441 tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa ccggtactga
    10501 tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac gccgacttcg
    10561 atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg gaagcgcgtg
    10621 cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca atcatcgttc
    10681 cgtctcagga cgttgttctg ggtctgtact atatgacccg tgactgtgtt aacgccaaag
    10741 gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc tctggtctgg
    10801 cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat gcaaacggtg
    10861 aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt ctgtggatga
    10921 ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa aaagcaatct
    10981 ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt atttttgcgg
    11041 accagatcat gtacaccggc ttcgcctatg cagcgcgttc tggtgcatct gttggcatcg
    11101 atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa gcagaagttg
    11161 ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc tacaacaaag
    11221 ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg gataacctgc
    11281 aaactgaaac cgtgattaac cgtgacggtc aggaagagaa gcaggtttcc ttcaacagca
    11341 tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt cagcttgctg
    11401 gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca atcaccgcga
    11461 acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt gctcgtaaag
    11521 gtctggcgga taccgcactg aaaactgcaa actccggtta cctgactcgt cgtctggttg
    11581 acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa ggtatcatga
    11641 tgactccggt tatcgaaggt ggtgatgtta aagagccgct gcgcgatcgc gtactgggtc
    11701 gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt ccgcgcaaca
    11761 cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac gcggttaaag
    11821 tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc tacggtcgtg
    11881 acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc gcggcacagt
    11941 ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt ggtgcggcat
    12001 ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc aagctcagca
    12061 acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt aatactgaac
    12121 tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct tacggtgcgg
    12181 tactggcgaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca aactgggacc
    12241 cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact gacatgatcg
    12301 acggtcagac cattactcgt cagacagacg aattgaccgg tctgtcttcg ctggtggttc
    12361 tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg aaaatcgttg
    12421 atgctcaggg taacgacgtt ctgattccgg gtaccgatat gcctgcgcag tacttcctgc
    12481 cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt gacaccctgg
    12541 cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg ccgcgcgttg
    12601 cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa atcagcggta
    12661 tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct tgttatcacc ccggtagacg
    12721 gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg ttcgaaggtg
    12781 aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac gacattctgc
    12841 gtctgcgtgg tgttcatgct gttactcgtt acatcgttaa cgaagtacag gacgtatacc
    12901 gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt cagatgctgc
    12961 gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa caggttgaat
    13021 actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg ggtgcaactt
    13081 actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc ttcatctccg
    13141 cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg ggcaaacgcg
    13201 acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg gcaggtaccg
    13261 gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct ccggctgcac
    13321 cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac gcaggtctgg
    13381 gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg
    13441 tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag gaatttctga
    13501 atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact aattgagtca
    13561 aactcggcaa ggattcgata ctattcctgt gtaactttct taaggaacga gaatgaaaca
    13621 ggaagtggaa aagtggcgac cttttggaca tccggatggt gatattcgtg atttatcatt
    13681 tcttgatgct catcaggctg tctacgttca gcatcatgag ggcaaagagc ctttagagta
    13741 tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac atcagacgaa
    13801 cgaagaaaaa caatcgttaa tgtaccacgc gcctaaagaa tctcgtccct tctgccagca
    13861 ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc cagaaagcaa
    13921 cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag acggaggaga
    13981 taaggcattt tactttgttg cgttcagggc tttcagggaa aagaaaaaac tccgtttgca
    14041 tgtaactagc gcttatccca tttctgaaaa acagaaaggt aaatcagtga aatttttcac
    14101 cattgcctac aacttattga gaaataagca gcttcctcag ccctcaaaat aacaaaaccc
    14161 accttaaggt gggtttcgcc agagaattat ctctggtatt cagaacgcca ttaccggact
    14221 ttgccttgac cttgcgataa tcgcaggttg cgggatgtct gaatttcttc agtctgctgc
    14281 atcctggaag atgagaacat gtgttcttat tttcgtctct atcatagttg agtatttact
    14341 ctcttacaat cagatctctt tcattgctca acaggcgatg gcttcagact ttgcattacg
    14401 gaatttttaa gaaaggcagg gcgaaacgag gaagaagctt ttcgccctgg acaacattcc
    14461 tgctgacggc actaccataa aaaaacacaa cgctttcaac aaccttgcgt ttttcatctt
    14521 ccgtagcggc tcggaataaa tttacgttct tacattccgt ctcatagtct ttgcgaggcc
    14581 cgtcccagat agctgtccca gtctttccat accggctgca aaccctgagc ggttaacgcg
    14641 gcagcaaccg cttccggtct gcgatcgtcg tgcggtgaga actgttccaa ctcggggtga
    14701 ttatcggcat agccacctgg ctgcgttttc gagaaggcgc tgacgttatt gatcgccagc
    14761 ggaataacgc gatcgcgaaa ccacggtgat tcccgcgtgg agagtgacag ttcaatctcc
    14821 ggtgcaagca gtcggaaggc gcagatggtt tgcactaact ggcgttcatc cataatcgac
    14881 gcaggctcaa tgccgccagt acacgggcgc aggcgcggaa aggagacaga gtaacggctt
    14941 tgccagtaat gctgttgcag ccatagcaaa tgttcggcaa ccatatagca gtcaacgcgc
    15001 cagttgtcgg aaaggccaat tagcgcgccg aggcctatct tatcaatccc cgcacgcccc
    15061 agccgatccg gcgtttccag ccgccagaag aagtcctgtt ttttgccttt cagatgatgg
    15121 cgggcataag tcgcctcgtg atatgtctcc tgataaacca tcacgccatc cagaccaagc
    15181 tgctttaact cggcgtattc cgtcttcgcc agcggttgca cttccatttg tagtgaagag
    15241 aactgttcac gcagggcggg gagatgacga cgaaagtaat ccatccccac tttcgcctga
    15301 tgttcaccag tgactaatag cagatgttca aagcccatct cccgtatagc ggcgctttcc
    15361 ctggcaatat ccgcttcatc cagcgttttg cgcttgatgc gattactcat ggaaaatccg
    15421 cagtacgtgc agtcgttagc gcaaagattg gaaagataaa gcgggacgta gaaactaact
    15481 gtgttgccaa atcgctgacg ggtcagacgc tgcgcccgtt gggccagttg ttccagatag
    15541 ccactggcgg ctggcgataa cagcgccatc atatcgtcgc gggtgaattg cgaggcattt
    15601 agcgcccgct ctacgtcaac agccgttttg ccgttgatac gcaggcggat gtcatcccag
    15661 tccagttgtc gccagcgatc gctgaaggtt ttcatgccga tgcctccaga aatccagtca
    15721 gcgggctggt ggcatgagca aaatgactgc ggctgcccgg tccggactga cgtgccagca
    15781 ggcctgcttc taccgccaga cgaaatgcct tcgccatgtt gacgggatcg tccgctacgg
    15841 caatcgccgt attcactaac accgcgtcgg cccccatttc cagggcctgc gctgcatggc
    15901 tgggaacgcc gatgccagca tcgacaacca ccggaactgt ggcctgctgg ataataatct
    15961 ccagcatggc acgggtttcc agtccctgat tcgagccaat cggcgcgccg agcggcatca
    16021 ccgctgcaca gccgacttct tccagacgtt tacacaatac cggatcggcc ccgcagtaag
    16081 gcagcacgac aaatccctgt tgtaccagca tttcggcggc tttcagagtt tcgatgggat
    16141 cgggcaacag ccagcgggcg tcagggtgaa tctctaattt taaccagttt gtgcctaacg
    16201 cttcacgtgc cagatgggcg gcgaaaatgg cttcttccgc tgttttcgcc ccggaagtat
    16261 ttggcagcag ggtcacaccc gccgcgataa gcggttcgag gatagcgtcg ttgtgctggc
    16321 gcaagtcgac acgtttcatc gccagtgtca ccagctggct gccggaagcg cggatctcct
    16381 ccaccatcag ttgtgaagaa gcgaatttcc ctgtgccggt aaacagatgt gaatcaaacg
    16441 ttttgtccgc aatacgtaac atctcaaccc cctgcaataa cctgaaaaag caggatctgg
    16501 tcgccatcct gcaggatatg ttgcgtccac tgctcacgcg ggacgatttg ctgattaatc
    16561 gccagagccg cgcccgcttg tcgttggtcg agttgctcca gtagttcgtg aacagtttgc
    16621 ccggtggcgc actgcatcgg ttgatcgtta aacaggatct gcattgcttc ctccgcatac
    16681 cgggcaacca ctggcgcggc gcaacgccag gctgcgccac tggctcgatt taccgtcgaa
    16741 cagtcggagt tctcccgcag gtgtctctat accgcttaat aacttaatgg cttccagtgc
    16801 ctgcaaagtg cccataaccc cgaccaccgg gccaaccacg cccgccgtgc ggcagttgcg
    16861 ttctggctcc tggttatctg gccacaggca gcggtaacac ccctgctccc agggcggcgt
    16921 cagtaccatc aactgaccgc caaatccgac cgcgctggcg gtgataagcg gcgtgttgag
    16981 taccacgcag gcggcattaa tctcctggcg agtcgccata ttgtcggtac agtcgagcac
    17041 cacatcggcc cgtgcaaccg catcttttaa cgcctcaccc gttaaccgtt gttgtaatgc
    17101 tgtcagttga atgtcgggat tcaactgtgt cagtcgctgt tggctgacct gcgatttcgg
    17161 gctgttgata tcttcagtgg taaagagaat ttgtcgttac agattgctta aatgcacatc
    17221 gtcgtcatct gccagtacca gcgtcccgac gccagcgccc gccaggtata gcgcagcagg
    17281 tgtacccagc ccgccaagac cgataatcag cacctggctg tcgagcagtt tttgctgccc
    17341 gtcaagagcg atatcatcga gcaggatttg gcggctataa cgcataaaat cacggtcatt
    17401 catcgccaac tcctgcaatt tccagcaact gtgccgttgc caaacgccag tctgcggctt
    17461 gagtaatggc gctgatgacg gcgatactgc cgacacccgt tgctatcacc gcaggcgcgc
    17521 gtgccagact gataccgcca atcgccacgg tgggataatc cgccagtcgc tcaacatgcc
    17581 gtgccagctg ttccagcccc tgcggtgcag aaggcatctg tttggtttgc gtcgggaaca
    17641 catgtcccag cgcgatataa gaggggcgtg ctgccagcgc gacgtcgatt tccatatcgt
    17701 catgtgtcga aacacccagc cgcaggcctg ccgcgcggat ggcattgaga tcggtggctt
    17761 gcaaatcttc ctgccccaaa tggacgccat acgcctgatg cttgatcgcg agccgccagt
    17821 aatcgttgat aaacaatcgc gcgttatagc ggcggcccag cgcaattgcc gccacgacat
    17881 cggcttccac ctcttcatcg cgccgatctt tgatgcgtag ctggagagta cgtacgcctg
    17941 catccaacag acgttcgatc cactgtacgc tgtccaccac tgggtacagt cctaaacgaa
    18001 aaggtacagg aggaaaatca ggctgataca tcacgcttcc tccttacgca ggtagatttc
    18061 tccgcctctg gcacggaagt tctccgacat atccgccatt cccatttcaa tagtttgcgt
    18121 ggcggcgtaa tcacgcactt cctggctgat tttcatcgag cagaatttcg gcccacacat
    18181 ggagcaaaaa tgggcgactt tacctgactc ttgcggcagg gtttcatcgt gataagcgcg
    18241 ggcggtaaac gggtcgaggg ccagattaaa ctggtcttcc cagcgaaatt cgaagcgggc
    18301 tttcgacatg gcgttatcgc gaatttgcgc gcccggatgc cctttcgcca ggtcagcggc
    18361 gtgggcggca atcttatagg tgataagccc ctgcttaaca tcttctttat taggcagacc
    18421 cagatgctct tttggtgtta cgtaacagag catcgcgcag ccaaaccagc caatcatcgc
    18481 cgcaccaatc cccgacgtga agtggtcata gcccggcgca atatcggtag ttagcggccc
    18541 cagagtgtaa aacggcgctt cgtggcagtg ctctaactcc tcggtcatat tgcggcggat
    18601 catctgcatc ggcacgtggc ctgggccttc aatcatcacc tgcacgtcat attcccaggc
    18661 aattttggtc agttcgccca gcgtatgcag ctcggcaaac tgcgcttcat cgttggcgtc
    18721 ctgaatagaa ccggggcgca gaccgtcgcc cagcgacagc gaaacgtcat aagcggcaca
    18781 gatttcacaa atttcgcgga agtgttgata gaggaaattt tcctgatgat gggagaggca
    18841 ccatttcgcc ataatcgaac cgccgcgaga gacgataccg gtcaggcgtt tcgcggtcat
    18901 cggcacatag cgcaacagta cgcccgcatg gatagtgaag taatccacac cttgctcggc
    18961 ctgttccagc agcgtgtcgc ggaacgcttc ccaggtaaga tcttcggcga tcccgttaac
    19021 cttctccagc gcctggtaga tcggcactgt accgatcggc accggactgt tacgcaaaat
    19081 ccactcgcgg gtttcgtgaa tatagcgacc ggtggagaga tccatcaccg tatccgctcc
    19141 ccagcgcgtg gaccatacca gcttttccac ttcttcttcg atggaagagg tgaccgccga
    19201 gttgccgata ttggcgttaa cttttaccag gaagttacgg ccaataatca tcggctccga
    19261 ttccggatga ttaatgttgg ccgggataat cgcacgtccg gcagcaactt catcacggac
    19321 aaattccgca gtgatatttt ccggcagacg tgcgccaaag ctcattcccg gatgctggtg
    19381 gcgtaaaact tcgctacgta tgcgctcgcg gcccatattc tcgcggatgg cgatgaattc
    19441 catttccgga gtgatgatgc cctggcgggc gtagtgcagt tgggtgacac ggcgtcctgc
    19501 tttggcgcgt tttggtgtta gtacgccgct aaaacgcagt tcgtcgaggc catcatctgc
    19561 cagccgcgct ttagtgtaat cggaactgcg cacggtaagt tcttcggtat cgccgcgcgc
    19621 atcgatccac ggctggcgta gttttgccag cccttgctgc acgttaatgg cgatctgcgg
    19681 atcgccatat gggccggagg tgtcgtagac cggaatcgct tcgttttctt caaactgcgg
    19741 ctgttctttg ctgccgccga ttagcgtcgg gctaagctgg atctcacgca tcggcacgcg
    19801 aacgccagag tgtgtgccag tgaggtaaat gcgttttgag ttgggaaagg cggtgccttc
    19861 cagggtgtcg ataaaatgtt gggcccgggc gcgttgttcg cggcgggtca gttttgttgc
    19921 agacatagct cattccaaaa agttaaggac gtggcttgtc agacgacgga tgaagcaaga
    19981 gacgatcgcc gcaaaggcga tgcgatagca gattaactct tgttcccttc gcaggtatta
    20041 gcctgatcag gttccgcgga tcccgaataa acggtctcag ccagttaagg cactccgaca
    20101 agaaattagc ccccacaaac ggggcgttga atgtaaatta cgcgttaaca gcgcagaact
    20161 caagcaggat gtttgacgcg ggcggcgtca agcaccagca gaatcagctt atcttccagc
    20221 acaaagcgcg cttccagcgc ctcgccgatg tcagataaaa ccttttgaaa ctcaaggtaa
    20281 ttatcatgat cgatggcggt ttccaggctg gaatcgtagt aatccataat ctgttgggta
    20341 ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt gccttccagc
    20401 ttatgaagaa tgcgctcata aatactgaaa tgtccggcag acaagtaatc gaccaggctc
    20461 tgacaaaaat catcaagggc tttttcgttt agcctcatgt acgattcttt gccaggctta
    20521 atgccaacca gattgtagta agccacgagc agatgcttac gtacatgtag ccagcgatca
    20581 accagtttgt tacttcctct gacgcgctcc gtcaggttat cgagctggtt aagcatgatt
    20641 gactccgcaa gtttgtattc aaaaactgct cagtgagaaa tgtaaaaacc atgttaaaca
    20701 tgccagtgat gcaaaggtag tgcaagagct atggatcgta taattgaaaa attagatcac
    20761 ggctggtggg tcgtcagcca tgaacaaaaa ttatggttac cgaagggaga attgccatat
    20821 ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg cgaatggcag
    20881 ggggaacctg tttggttagt acaacagcag cggcgtcacg atatggggtc ggtacgtcag
    20941 gtcattgatc tcgatgttgt gctgtttcaa ctggccggac gaggcgtaca actggcggag
    21001 ttttaccgat cgcataaata ctgtggttac tgcgggcatg aaatgtatcc gagcaaaacc
    21061 gaatgggcga tgctgtgcag ccattgccgt gagcgttact acccgcaaat cgccccctgc
    21121 attattgttg ccatccgtcg cgatgattcg atcctcctcg cccagcatac ccgccatcgt
    21181 aacggtgtcc atacagtact tgccggattc gtcgaagtgg gcgaaaccct cgagcaggca
    21241 gtcgcgcggg aagtgatgga agagagcgga attaaagtta aaaacttgcg ttacgtgact
    21301 tctcagccgt ggccgtttcc tcagtcttta atgaccgcgt ttatggcgga atatgacagc
    21361 ggcgacatcg tgatcgaccc gaaagaattg ctcgaggcga actggtatcg ctatgacgat
    21421 ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga tactgtggcg
    21481 atgtgtcggg cagagtatga gtgatgatac actgaccgcc tgacgcacta aggaacagcc
    21541 aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca gcccgttgat
    21601 gtcactccag tatggatgat gcgccaggcg ggtcgctatc taccggaata taaagtcacg
    21661 cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaacg ccgagctggc gtgcgaagtg
    21721 actttgcaac cgctgcgtcg ctacccgctg gatgcggcga tcctcttttc cgatatcctc
    21781 accgtgccgg acgcgatggg gttagggctc tattttgaag ccggagaagg tccgcgtttt
    21841 acctcgccag tcacctgcaa agccgacgtc gataagctgc caattccgga cccggaagat
    21901 gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc gcgaactgaa aggcgaagtg
    21961 ccgctgattg gtttttccgg cagcccgtgg acgctggcaa cctacatggt ggaaggcggc
    22021 agcagcaaag ccttcaccgt gatcaaaaaa atgatgtatg ccgatccgca ggcgctgcac
    22081 gctctactcg ataaactggc gaaaagcgtc actttgtatc tgaatgcgca gattaaagcc
    22141 ggtgctcagg cagtgatgat tttcgacacc tggggcggtg tgcttaccgg gcgcgattat
    22201 caacagttct cgctctatta catgcataaa attgttgatg gtttactgcg tgaaaacgac
    22261 ggtcgccgcg taccggtcac gctgtttacc aaaggcggcg gacagtggct ggaagcgatg
    22321 gcagaaaccg gttgcgatgc gttgggcctc gactggacaa cggatatcgc cgatgcgcgc
    22381 cgccgtgtgg gcaataaagt cgcgttgcag ggtaatatgg atccgtcgat gctgtacgcg
    22441 ccgcctgccc gcattgaaga agaagtagcg actatacttg caggtttcgg tcacggcgaa
    22501 ggtcatgtct ttaaccttgg tcacggcatt catcaggatg tgccgccaga acatgctggc
    22561 gtgttcgtgg aggcagtgca tcgactgtct gaacaatatc accgctaagg agtgattatg
    22621 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ccgcgaggat
    22681 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    22741 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    22801 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    22861 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    22921 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggtttg
    22981 atggtggatg tgccaacgat cggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    23041 ctctccagcg aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    23101 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    23161 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    23221 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    23281 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaacttaa catgttacaa
    23341 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    23401 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    23461 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    23521 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    23581 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggtgtt aagtgaactg
    23641 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    23701 atcacgaccg ttgcgatgct ggaaatgact caggttggtc gcgaaatgag cgatgaagag
    23761 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    23821 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    23881 gaggcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    23941 aacgcctgat ttgtcgtact tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    24001 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    24061 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    24121 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    24181 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    24241 gttggtttcg gtaccttcaa agtgaaccac cgcgctgagc gtactggccg caacccgcag
    24301 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    24361 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    24421 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg ctggcgctct gcttctcact
    24481 gcctgtagtc ataactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    24541 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    24601 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    24661 acgctcatca atatcaatgt ttacagcaaa ccgccggtga atattcgcgc gcgttttgac
    24721 gatcgcggtg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    24781 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    24841 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    24901 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    24961 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    25021 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    25081 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    25141 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    25201 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    25261 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    25321 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    25381 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    25441 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    25501 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    25561 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    25621 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    25681 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    25741 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    25801 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    25861 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    25921 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    25981 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    26041 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    26101 ttccccgcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    26161 tagcgcagac ggtgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    26221 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    26281 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    26341 tatcctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    26401 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    26461 ggcactgggg catctggcgg caggcgttgc ccacgaaatc cgtaatccac tttcctcaat
    26521 taaagggctg gcgaaatact ttgccgaacg cgcgccagca gggggagaag cgcatcaact
    26581 ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg agttgctgga
    26641 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    26701 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    26761 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    26821 tctctatctc aatgctattc aggcgattgt tcagcatggc gtgattagcg tgacggtcag
    26881 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    26941 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    27001 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    27061 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    27121 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    27181 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    27241 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    27301 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    27361 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    27421 ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    27481 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    27541 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    27601 gcgccatcgg aagccacagt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    27661 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    27721 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    27781 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    27841 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    27901 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    27961 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ctaggatctc
    28021 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    28081 atccctctgc tggctggcca ttttctgcag cgctttgccg agcgtaatcg caaggcggta
    28141 aaaggtttta cgccccaggc gatggatctg ttgattcatt acgactggcc gggaaatatt
    28201 cgtgagctgg aaaacgcggt ggaacgggca gtggtgctgc tgccggggaa tatatttccg
    28261 aacgcgagct gccgctggcg attgccagta cgccgatccc gctggggcaa agtcaggata
    28321 ttcagccgct ggtggaagtg gaaaaagagg tgattctggc ggcactggag aaaacgggcg
    28381 gcaacaaaac tgaagccgcc cgtcagttag ggatcacgcg taaaacgtta ttggcaaaac
    28441 tgtcgcgtta gttctgctcg cgttcgatag cacgccagcc gatatctttc cggcagaagc
    28501 agtcgtccca gtggatatcg gtcattaagg cataggcgcg tttctgcgct tctgccacgg
    28561 tatgacccag cgcggtgacg cacagtacgc gcccgccgtt ggtcactacc tgctcgtcat
    28621 ccgccagttt tgtgcccgcg tggaacactt tgccgcctgc cacttcttcc agcagcaggc
    28681 cgtggatcac gtcaccggtg cggtaatcac ccggatatcc acccgcagcc atcaccacgc
    28741 cgagagaagc gcgttcatcc cactcggacg ttttctcgtc cagtttgctt tcacaggccg
    28801 ccaggcagag ctcaaccaga tcggacttca tgcgcagcat aatcggctgg gtttccggat
    28861 cgccaaagcg gcagttaaat tcaataacct tcggattgcc ctgtttgtcg atcatcaggc
    28921 ccgcgtagag aaaaccggtg taggtgttgc cttccgccgc catgcctttc acggttggcc
    28981 agataatacg ttccatggtg cgctgatgaa cgtcatcggt tactaccggc gcgggggagt
    29041 aagcgcccat cccgccggtg tttggtccgg tatctttatc gcctacgcgt ttgtgatcct
    29101 ggctggtagc catcggcagc acatgctcgc cgtccaccat cacgataaag ctcgcttctt
    29161 cgccatcgag gaactcttcg ataacgatgc gatgacccgc gtcgccaaaa gcgttgcccg
    29221 ccagcatatc gtgaacagcc gcttccgctt cttccagcgt catcgccacg ataacgcctt
    29281 tcccggcagc cagaccgtcc gctttaatga cgattggcgc gcctttctca cgcagatacg
    29341 ccagcgcagg ttctacctcg gtgaagttct ggtattccgc cgtagggatc ttatggcggg
    29401 ccaggaaatc tttggtaaac gcttttgagc cttccagttg ggccgcacct gcggttgggc
    29461 cgaagatttt cagcccggcg gcgcggaagg tatcgaccac gcctttcacc agcggcgctt
    29521 ccgggccgac gatggtcaga tcaatctttt cgttttgtgc gaaatccaac agcgccggga
    29581 tatcggtcac gccaatagca acgttttgca gcgcgggttc cagcgcagtg cctgcattac
    29641 ccggagcaac aaaaacagtc tcaaccagcg gcgactgggc cgctttccag gccagcgcgt
    29701 gctcgcgccc gccgttacca atcactaata ctttcatcta ttgctccatt aatggcggaa
    29761 gtggcgcatg tcggtgaaga gcatcgcaat accgtgctcg tcggcggcgg caatcacttc
    29821 gtcatcacgg atggaaccgc caggctggat tacgcaggtc acgcccgcag cggcggcggc
    29881 atcaataccg tcgcggaacg ggaagaacgc gtcagaagcc atcgaggaac ctttcacttc
    29941 caggccttca tcggcagctt taataccggc gattttcgcg gagtacacgc ggctcatctg
    30001 gcccgcgcca ataccgatgg tcatattgtt tttggcatag acgatagcat tggatttcac
    30061 gaacttcgcc actttccagc agaacagcgc atcgcgcagt tcctgttcgg tcggctgacg
    30121 ttgggtgacg acgcgcagtt cttccgcacc gaccatcccc aggtcacgat cctgaaccag
    30181 cagaccgccg ttcacgcgtt tgaaatcaag acccggaaca cgctcgcccc actgaccgca
    30241 ggtcagaacg cgtacgttct gcttggcggc ggtgattttc agggcttctt cgctggcgga
    30301 aggcgcaata atcacttcga caaactgacg agaaatgatg gcctgtgcgg tttccgcatc
    30361 tagctcgcgg ttaaaggcaa tgatgccgcc gaatgcggag gttgggtcgg ttttgtacgc
    30421 gcgatcgtaa gcatcaagaa tggaattgcc gatagctaca ccgcaagggt tggcgtgctt
    30481 cacaatcaca catgccggct cggcgaactc tttcacgcac tccagcgccg catcggtatc
    30541 ggcgatgttg ttataagaga gggctttacc ctgaacctgg gttgcggtag caacggaggc
    30601 ttctttcaca ttctcttcta tatagaaggc agcctgctgg tggctgttct cgccgtaacg
    30661 catatcctgc ttcttaatga agttcaggtt cagcgtgcgt gggaagcgac cggcggcttc
    30721 tttgctttca ccgtggtacg ccggaaccat gctgccgaag tagttggcaa tcatgctgtc
    30781 gtaggcggca gtgtgttcga aggctttgat ggcgaggtcg aaacgggttg caagcgtcag
    30841 cgatccttcg ttgtcatcca tctctttaat aatggcgtcg tagtcgctgc tcttcaccac
    30901 gattgcgaca tctttatggt tcttagcggc ggagcgcacc atggtcgggc cgccgatatc
    30961 gatgttctca accgcatctt ccagcgagca gccttcacga gcaacagtct gggcgaacgg
    31021 atacaggtta acaaccacca tatcgatagg ctggatctga tgttcttcca taatggcatc
    31081 gtcctggccg cgacggccca gaatgccacc atgtactttc ggatgcaggg tcttcacgcg
    31141 tccatccatc atctccggga aaccggtgta atcggaaact tcggttaccg gcagaccttt
    31201 ttctgctaac agacgggcag tgccccctgt agacagcagc tccacaccgc gtgcggaaag
    31261 tgcctgggcg aattcgacga taccggcttt gtcagaaaca ctgagcagag cgcggcggac
    31321 tggacgacgt tgttgcatgg taaatcccct ggatttgact attacagaga gcgttagctg
    31381 aatttttcgc gaaaaactca gctaacgccc ctaacggggc atccttattt ttcgcccgca
    31441 ttgtaacgaa aacgtttgcg caacgctcgc gaatttttct ctttcaatgg tgatcacaat
    31501 tttgacagtg gctaccgtgg gaaaaataca gaaattacat tgatgattgt ggataactct
    31561 gtgcgtaaaa aggtataaag cggggttttg ctggggaatg cagcagtcag tcatttttct
    31621 gcaatttttc tattgcggcc tgcggagaac tccctataat gcacctccat cgacacggcg
    31681 gatgtgaatc acttcacaca aacagccggt tcggttgaag agaaaaatcc tgaaattcag
    31741 ggttgactct gaaagaggaa agcgtaatat acgccacact cacgacagtg cgctaaagct
    31801 cgtcgcaact gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga
    31861 ttcttaacgt cgcaaga
//
LOCUS       DAJVTY010000028        31361 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVTY010000028 DAJVTY010000000
VERSION     DAJVTY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31361
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.028"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(5..3286)
                     /locus_tag="OQQ66_001180"
     CDS             complement(5..3286)
                     /locus_tag="OQQ66_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1904183.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3404..4597
                     /locus_tag="OQQ66_001181"
     CDS             3404..4597
                     /locus_tag="OQQ66_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1904184.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4661..5914)
                     /gene="glyA"
                     /locus_tag="OQQ66_001182"
     CDS             complement(4661..5914)
                     /gene="glyA"
                     /locus_tag="OQQ66_001182"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1904185.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6242..7432
                     /gene="hmpA"
                     /locus_tag="OQQ66_001183"
     CDS             6242..7432
                     /gene="hmpA"
                     /locus_tag="OQQ66_001183"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1904186.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7477..7815)
                     /gene="glnB"
                     /locus_tag="OQQ66_001184"
     CDS             complement(7477..7815)
                     /gene="glnB"
                     /locus_tag="OQQ66_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1904187.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7876..9210)
                     /gene="glrR"
                     /locus_tag="OQQ66_001185"
     CDS             complement(7876..9210)
                     /gene="glrR"
                     /locus_tag="OQQ66_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1904188.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9200..9913)
                     /gene="qseG"
                     /locus_tag="OQQ66_001186"
     CDS             complement(9200..9913)
                     /gene="qseG"
                     /locus_tag="OQQ66_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1904189.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10078..11505)
                     /gene="qseE"
                     /locus_tag="OQQ66_001187"
     CDS             complement(10078..11505)
                     /gene="qseE"
                     /locus_tag="OQQ66_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1904190.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGVVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12063..15950)
                     /gene="purL"
                     /locus_tag="OQQ66_001188"
                     /gene_synonym="purI"
     CDS             complement(12063..15950)
                     /gene="purL"
                     /locus_tag="OQQ66_001188"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1904191.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16210..17766
                     /gene="mltF"
                     /locus_tag="OQQ66_001189"
     CDS             16210..17766
                     /gene="mltF"
                     /locus_tag="OQQ66_001189"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1904192.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17763..18299)
                     /gene="tadA"
                     /locus_tag="OQQ66_001190"
     CDS             complement(17763..18299)
                     /gene="tadA"
                     /locus_tag="OQQ66_001190"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1904193.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18324..18959)
                     /gene="yfhb"
                     /locus_tag="OQQ66_001191"
     CDS             complement(18324..18959)
                     /gene="yfhb"
                     /locus_tag="OQQ66_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1904194.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19168..20016
                     /locus_tag="OQQ66_001192"
     CDS             19168..20016
                     /locus_tag="OQQ66_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1904195.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20072..20332
                     /gene="yfhL"
                     /locus_tag="OQQ66_001193"
     CDS             20072..20332
                     /gene="yfhL"
                     /locus_tag="OQQ66_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1904196.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20526..20606)
                     /gene="shoB"
                     /locus_tag="OQQ66_001194"
     CDS             complement(20526..20606)
                     /gene="shoB"
                     /locus_tag="OQQ66_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1904197.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21026..21406)
                     /locus_tag="OQQ66_001195"
     CDS             complement(21026..21406)
                     /locus_tag="OQQ66_001195"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1904198.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21406..22137)
                     /gene="pdxJ"
                     /locus_tag="OQQ66_001196"
     CDS             complement(21406..22137)
                     /gene="pdxJ"
                     /locus_tag="OQQ66_001196"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1904199.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22149..22877)
                     /gene="recO"
                     /locus_tag="OQQ66_001197"
     CDS             complement(22149..22877)
                     /gene="recO"
                     /locus_tag="OQQ66_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1904200.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22889..23794)
                     /gene="era"
                     /locus_tag="OQQ66_001198"
     CDS             complement(22889..23794)
                     /gene="era"
                     /locus_tag="OQQ66_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1904201.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23791..24471)
                     /gene="rnc"
                     /locus_tag="OQQ66_001199"
     CDS             complement(23791..24471)
                     /gene="rnc"
                     /locus_tag="OQQ66_001199"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1904202.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24744..25718)
                     /gene="lepB"
                     /locus_tag="OQQ66_001200"
     CDS             complement(24744..25718)
                     /gene="lepB"
                     /locus_tag="OQQ66_001200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1904203.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25734..27533)
                     /gene="lepA"
                     /locus_tag="OQQ66_001201"
     CDS             complement(25734..27533)
                     /gene="lepA"
                     /locus_tag="OQQ66_001201"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1904204.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27731..28210)
                     /gene="rseC"
                     /locus_tag="OQQ66_001202"
     CDS             complement(27731..28210)
                     /gene="rseC"
                     /locus_tag="OQQ66_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1904205.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28207..29163)
                     /gene="rseB"
                     /locus_tag="OQQ66_001203"
     CDS             complement(28207..29163)
                     /gene="rseB"
                     /locus_tag="OQQ66_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1904206.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29163..29813)
                     /gene="rseA"
                     /locus_tag="OQQ66_001204"
     CDS             complement(29163..29813)
                     /gene="rseA"
                     /locus_tag="OQQ66_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1904207.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29846..30421)
                     /gene="rpoE"
                     /locus_tag="OQQ66_001205"
     CDS             complement(29846..30421)
                     /gene="rpoE"
                     /locus_tag="OQQ66_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1904208.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30418..30573)
                     /gene="rseD"
                     /locus_tag="OQQ66_001206"
     CDS             complement(30418..30573)
                     /gene="rseD"
                     /locus_tag="OQQ66_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1904209.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30829..>31361
                     /locus_tag="OQQ66_001207"
     CDS             30829..>31361
                     /locus_tag="OQQ66_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1904210.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7496 a         8402 c         7918 g         7545 t
ORIGIN      
        1 gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat gccgctttcg
       61 gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc gcaacctccc
      121 cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta
      181 aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc gcctcaatac
      241 tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggttt
      301 gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat
      361 cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac
      421 gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag aaccagatgt
      481 cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg
      541 catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta
      601 ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtgga
      661 atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat
      721 tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac
      781 gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc cgttcgaaaa
      841 gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct
      901 cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg tcggcaaact
      961 ccggcgacat ctctacgcaa cgttgccaaa aggcaatggc tttgttgtag ctacgtttgt
     1021 tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata ctctccagcg
     1081 ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca tcaatggctt
     1141 ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg taaagcggca
     1201 gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc attccccagt
     1261 taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac agaccgcgcc
     1321 actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac agagtggcgt
     1381 tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc tgacgaccac
     1441 ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg caggcgcgaa
     1501 gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc gccagtcgtg
     1561 ccaggccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc cagaaatcct
     1621 cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc aaactcgcct
     1681 gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagagcctga gtggcatacg
     1741 ccaccgcttg cgggaaatcg gcgcggttat attccagcat cgccagcgcc aggttacagc
     1801 ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt gaacggctgg
     1861 cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta ctggtaatgt
     1921 cttgtgctgc cagtggcgct ttggcgacgt ccagcaacgg caactcttgc ggctgatgtt
     1981 cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc agcctttcag
     2041 gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc acggcatcat
     2101 caagtaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg ttcaacggag
     2161 agatggcata cagcccccac tcaatccccc gcttactacg ctggagtttt atcaccgcat
     2221 cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac tgctcaaaac
     2281 gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca aaaataccgg
     2341 tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac gcctggccaa
     2401 attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg ttggcaacgt
     2461 gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt
     2521 cggccatata tgaggttgta acaggcacat ttttatagca agaaatgtcc actccggcgg
     2581 agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg gcccgtttgc
     2641 cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct tccccccctt
     2701 tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg ttatagacgc
     2761 ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta
     2821 aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct tcatgggctt
     2881 cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc cagttaaact
     2941 caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta atgacttcat
     3001 tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc cgaccgccca
     3061 gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgccaggatt
     3121 tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg
     3181 atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat
     3241 aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat
     3301 taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt
     3361 tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa
     3421 caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa
     3481 gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa
     3541 tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg aaagccagac
     3601 gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga cgctgtgcct
     3661 gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg
     3721 tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt ccgaaatcga
     3781 aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc tggcgctggc
     3841 aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc aagcgccgtg
     3901 gacaacgccg gttgaggtga agtatctgct ggaagagaag ctgggcattc gggtgatggt
     3961 cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt cgcaggaacg
     4021 ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca
     4081 aatttatcgc ggcagtttgt atggtagcgg acaaattggt cacaccatcg ttaatccgga
     4141 tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc
     4201 attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttgatcc
     4261 tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac cgtggatcac
     4321 cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact tcctcaacat
     4381 cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agaactggct
     4441 taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag gaccgagcgt
     4501 gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc tgggaattgg
     4561 ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg tagaaaggct
     4621 tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa accgggtaac
     4681 gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca tcattgatgc
     4741 tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct ttgaagccgc
     4801 gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg ctcttcggat
     4861 cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg gcgtctgctt
     4921 ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta tcagtgccgc
     4981 cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta gcgtttttcg
     5041 cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga gcaaccgctt
     5101 taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg gcagagttca
     5161 gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg cgcggacccg
     5221 ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg ttcgggtaga
     5281 cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa gcaccgatgc
     5341 tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca gagaaaccac
     5401 cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg tagtcgatat
     5461 gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag aagttaaccg
     5521 gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg gtatcacctg
     5581 gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc ggctggacgt
     5641 tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt tcaacgatat
     5701 caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca tatttgttgg
     5761 tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg gaggcgatca
     5821 gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac agttcggcat
     5881 cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc agctaacaat
     5941 aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat aaccgaatca
     6001 ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc aggctacgca
     6061 aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg tttgtgatgc
     6121 aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt acattgcagg
     6181 gctatttttt ataagatgca tttgaaatac atcaattaag atgcaaaaaa aggaagacca
     6241 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
     6301 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
     6361 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
     6421 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
     6481 aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca tcgtcggtga
     6541 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
     6601 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
     6661 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
     6721 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
     6781 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
     6841 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtattgc
     6901 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
     6961 cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg cagatgacac
     7021 accagtgacg ttaatctctt ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
     7081 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
     7141 tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc gctttaccgc
     7201 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
     7261 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
     7321 tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg atctgggcgt
     7381 gaagcaggaa aacatttatt acgaatgctt tggcccgcat aaggtgctgt aatttgatgt
     7441 tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt cgtcctcttc
     7501 accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac cgtcaccgat
     7561 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
     7621 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
     7681 cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg tcatgccggt
     7741 aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt ttataatcgc
     7801 atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct gctaaacgta
     7861 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
     7921 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
     7981 ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg cctcaacaaa
     8041 ggttggcagc gccgtgtttt caccctccag cgcctgctct actaatgcat cgctaataac
     8101 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
     8161 cggccagcta gcggtcatca ggcgtttcat cgcatcagtg gagaacgcgc ggacaaacgg
     8221 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agaagcggaa tgtcttctgt
     8281 gcgctctgcc agagccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
     8341 gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg tgagtggcag aaataatccg
     8401 cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
     8461 gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga gaaatagcgt
     8521 tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc
     8581 acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc cacagttaat
     8641 agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
     8701 tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg ccaccagccg
     8761 cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct cgcgccagcg
     8821 ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat atagcgcgtc
     8881 tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
     8941 ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca ctttctggat
     9001 ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
     9061 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
     9121 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
     9181 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
     9241 gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
     9301 gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
     9361 gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac tgctggagtt
     9421 tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
     9481 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
     9541 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
     9601 aggtattttg ccagcagccg tcgtcatatt ggcgagcttg ctgacgcgac tgcgcaggca
     9661 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt
     9721 taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc agttgataaa
     9781 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc tgcacgcaac
     9841 ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa
     9901 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
     9961 agtctggaca tggacagcgc ggagaaaccg gagcgtacat atcgtacgtg aagatttcga
    10021 gcactgcccg gagccgaact gacaaataaa atagcctgat aggataggct gttagcatta
    10081 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    10141 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    10201 accgctgccc ttcaccaccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    10261 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    10321 cgcaccatgt gaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    10381 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    10441 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    10501 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    10561 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc ggctgctgct
    10621 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctggtcagc
    10681 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    10741 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat
    10801 acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat tgcccagaga
    10861 acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc cgataatcat
    10921 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    10981 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    11041 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    11101 ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt tcaaatcctg
    11161 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    11221 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca
    11281 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt cactacgccg
    11341 ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg cattcaggct
    11401 ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa atgccagcat
    11461 taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt tactctcgtc
    11521 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    11581 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    11641 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    11701 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct tactattatg
    11761 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    11821 ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac
    11881 agcttaagat aaacttatta gattacaata aaatcaatca cttaagtgtc gccagaaagc
    11941 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc ggagttggga
    12001 gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa tgaactgatg
    12061 aattacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc atcctcgccc
    12121 cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc
    12181 ataatggtga ctcgaccact ttcagtcgtg actgccgtaa taccgttcgg tgaaccgttc
    12241 gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg cagtgccacc
    12301 agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac gcgcccttca
    12361 ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacaa cagagacggg
    12421 ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt attgcgcaca
    12481 aaacgtggcc acaactcact acctgggatc agttcgcgca ggttagacat catctggcaa
    12541 ccgttacata cccccagcgc cagcgtttgc ggacggtgga agaaggttgc aaactcatcg
    12601 cgtacacggt cattgaacag gattgacttc gcccaacctt caccggcacc cagcacatca
    12661 ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat cttccaggcc cgtgcgtccg
    12721 gtcagcaggt cactcatatg cacgtcgata gcatcaaagc ctgcacggtg gaaagctgcc
    12781 gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt cggacgtgcg
    12841 ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag ttttacattc
    12901 aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca ctccgggttg
    12961 tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa cgtggtgcgg
    13021 ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc
    13081 tgccctacat aatggacaca atcagcaagc ccatgctgtg ccagtacgga ctcgaccgct
    13141 tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa caacgccgcc
    13201 aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc cgcaatgacc agcaaaggcc
    13261 atttccgcca gcgttaccag caggccgcca tcagagcggt cgtggtatgc gagcagctta
    13321 cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttgcgc gacgtcgcgt
    13381 acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt tgcgcccagc
    13441 gcgttattgc ctttgcccaa atcaatcagc aatagtgcgt tatcttcggt agaaagctgt
    13501 ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg cagaaatcac cagcgacagc
    13561 ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat cgacatagag
    13621 tctttaccca ccgggatcgt caggcccagc gccggacaaa gctcttcgcc cacggcttta
    13681 acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat ccagttggcg
    13741 gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt taacgcttca
    13801 ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca
    13861 atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg
    13921 accggcacct gccacggacc caccatctga tcgcgcgcta ccatgccagt tacgctgcgg
    13981 tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag tatgcgtttc
    14041 accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc tttcagcgtt
    14101 tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg cagatcgatc
    14161 ggctgattat caaaatgacg atcgtgtaga gaaagatgca gttcttcggt cgcttcacca
    14221 atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg taattgatcg
    14281 gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc cagcgggctc
    14341 atgcctggct cgtcgctcag aatgtcgcgc agttcaaatt taccgccacg cccgccgtcg
    14401 ctcaccagtt ccggcatagc gttagaaaga ccgcctgcgc caacgtcgtg gataaacagt
    14461 attgggttgg catcaccaag ctgccagcaa cggtcaatca cttcctggca gcgacgctcc
    14521 atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga ctgaccagac
    14581 gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc gccgagaacg
    14641 accagcttcg cgccaacgtt gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg
    14701 atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc gttgtggctg
    14761 ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc aaattcgttg
    14821 ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt gacaatgcgc
    14881 tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caagaattcg caggttggaa
    14941 acggagaaac caaccaaacc cgctttcggc ttcgcgccgc gcccggtggc accttcatcg
    15001 cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg
    15061 ttgtgagttt cgactttcat cagaatatgc gccggttcct gatggaaatc atagcggccc
    15121 gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc ggcgttatct
    15181 ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat catcctgaac
    15241 agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat tttgtggcgg
    15301 cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg gttacgacca
    15361 agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc caggccaaga
    15421 cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac gcttgtgacc
    15481 ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa aaagacagtt
    15541 tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt ggtcagcgta
    15601 ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag
    15661 ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg acgcggggtc
    15721 accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata tttcagcagg
    15781 cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc aaaatggaca
    15841 tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg
    15901 ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg
    15961 tcttctaagc tttcggtgta cccaaggggg ggggaaacgg gcgtcattat aaagaatctg
    16021 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    16081 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    16141 atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga
    16201 attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg
    16261 ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc
    16321 gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac
    16381 gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac
    16441 ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctt
    16501 gataatggta acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa
    16561 aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt
    16621 cagtatcgcc cacgtacgct gtgcaacctg acggcggagc aactcaccgt tgcaccgggt
    16681 catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga attaagctgg
    16741 aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc
    16801 gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc
    16861 gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc
    16921 gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg
    16981 ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg
    17041 cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa
    17101 tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg
    17161 gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc
    17221 gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg
    17281 cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc
    17341 tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg
    17401 acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta
    17461 cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg acatgaagcc
    17521 tacgcttatg tcgaaaatat tcgtaagtat caaattagcc tggtgggtta tctgcaagag
    17581 aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg
    17641 cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca
    17701 aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa
    17761 aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga
    17821 aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact
    17881 cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc
    17941 gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac
    18001 attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc
    18061 accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca
    18121 atcgggcggt tccaaccttc gccgattacc cgattgttat gcactaatac cgcgccgacc
    18181 ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat
    18241 tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt
    18301 atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact
    18361 cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca
    18421 ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc
    18481 agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga
    18541 ttaacccgcg gcagccaggg cttatcgaaa taaaccgctt caaccagcgg ctgcggagag
    18601 ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga
    18661 accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc
    18721 tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag
    18781 cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca
    18841 agtaacgcat tcagcggttg gcgacgtagt aaatagcgca gaaaactgcc gaacatatcc
    18901 tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa
    18961 ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat
    19021 agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca
    19081 tggcaattag cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt
    19141 cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac
    19201 caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg
    19261 gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc
    19321 gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt
    19381 gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt
    19441 gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac
    19501 gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct
    19561 gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc
    19621 tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc
    19681 gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta
    19741 cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg
    19801 attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt
    19861 gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta
    19921 acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt
    19981 cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc
    20041 gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta aaaaatgcat
    20101 caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg gagatcatat
    20161 ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga caccaacctg
    20221 ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg tcgagacaga
    20281 agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt aatattctac
    20341 tctggaagta gagtattaat tatattactg ggaagcctta acgccattat atttatttaa
    20401 ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc caagacactt
    20461 atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa gtacgtttcc
    20521 ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt gatgacccgt
    20581 ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg cagccgttca
    20641 gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg catgctgcac
    20701 tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt ggcatggtgt
    20761 aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga attggcggtc
    20821 acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt atgctaaaga
    20881 ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt tgagggtgca
    20941 tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt cgggggtttt
    21001 actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca caagcatagt
    21061 ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc gccagtttta
    21121 atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat acttcaaatt
    21181 gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg gcttctttca
    21241 cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag attgcccatt
    21301 cgttatcgct taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc actgcttcta
    21361 tgcgagcgat ctctacgata tccgtgccta aacctaatat tgccattagc cacgcgcttc
    21421 cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca ctgcacggcc
    21481 aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa tggctttcac
    21541 gttgtgatag gtcagaccgt gtccggcgtt aactttcaga ccgagactag cggcaaaggt
    21601 cgcggctttg gcgatacgca ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc
    21661 atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag ctttgatctg
    21721 ctcttcatcg gcgtcgataa agagagaaac ctgaatcccg gcatctgtca gacgtttgca
    21781 ggcatcgcgc attttttcac gctgccctgc aacgtccagg ccgccttcgg ttgttacttc
    21841 ctgacgcttt tccggtacca ggcagcaaaa atgtggcttc gtctcaacgg cgatcgccag
    21901 catctcttcg gtcactgcca tctccagatt cattcgggta tccagcgtct gccgcaggat
    21961 gcgcacatcg cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg taatgccgtc
    22021 cgctcccgcc tgctcggcaa taaacgccgc ctgcaccgga tccgggtaag cagtaccgcg
    22081 tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt cagccatgac
    22141 aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg ccggaacagt
    22201 tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt
    22261 ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc ttttaactgc
    22321 cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt ttcttcgcga
    22381 taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcgcaata ggtaaaattg
    22441 acgccatagc ccagatgccc gagcagtgcc agttcaaagc ggcgcagcgc gggttctggc
    22501 gtaccagtgg cacctgcaag agactgaatg cagtgcaaat aatcgaaaaa gagttcagag
    22561 aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag accgctgtaa
    22621 agcgtgatac cgcttaatgg cagcgccagc gagacagctt cagcactgcg cagcgttttg
    22681 acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa tgcacctttc
    22741 agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc cgattcctcc
    22801 gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag gacaaatgcg
    22861 cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg agactgcgca
    22921 gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca aggtggacag
    22981 gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt ttgatcttgg
    23041 cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga atcaaaccat
    23101 tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg gtcacggagt
    23161 acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca gacgccataa
    23221 aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct tcaggcagat
    23281 gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca gagatcggca
    23341 cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc agatcggctt
    23401 tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg ccatcgcgca
    23461 gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg acaaaaatca
    23521 ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg ttaatagcgc
    23581 gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac gcgccttcag
    23641 tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg atggagatct
    23701 tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt ccgacgatgg
    23761 caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt tcaacgcctg
    23821 ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca ccggttcact
    23881 caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc cacgtacctg
    23941 gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt gcaagcgcgt
    24001 tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt gataccagtt
    24061 gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac caattaatgc
    24121 ttcgacggta tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa gttcaccagg
    24181 ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc
    24241 acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacgag ggaaacggtg
    24301 ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc
    24361 gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt cctgatgatt
    24421 aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca tgcgatacca
    24481 ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat ggctctgcta
    24541 actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt tcgtgtgccg
    24601 tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac aacgacgggg
    24661 gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc cataacggcg
    24721 acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa gcgcacacct
    24781 gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc ccgaccgacc
    24841 agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc gcggttgtcg
    24901 cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg ccctggctgt
    24961 tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg gtgcgtcaca
    25021 tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt ttcgtttttc
    25081 ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg
    25141 aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca cgcctggccg
    25201 gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt gactttatcg
    25261 cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg atatttaaag
    25321 accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg gtagatagga
    25381 tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa cagagtcggc
    25441 atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac aatcaatacg
    25501 atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt cggcgcaacc
    25561 tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt
    25621 tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc cgtcaccagt
    25681 gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa gggttatttg
    25741 ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc gacgttaccg
    25801 atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt cttacggctg
    25861 atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac ggtggatcgc
    25921 gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg gcgtgggatc
    25981 agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc acggtgggtg
    26041 atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg taccatgtcg
    26101 gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga ggtagatttc
    26161 aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt cagcgccacc
    26221 tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca cagcgtaata
    26281 acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg ctcgcgcagt
    26341 tcgtaaatgt tatttaccgc aggcagcttg gatgggctgt cgacgtagat aacttccctt
    26401 gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc cagatcgtat
    26461 tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccaaggaa gccgcagcgg
    26521 aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaggc atcattcagg
    26581 ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac cgggaacaga
    26641 ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc tgccggatta
    26701 cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat cgcacatacc
    26761 agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg cgtgaagatg
    26821 cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt gtcgccctta
    26881 cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt gtcgaaccat
    26941 gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg cggaatgtcg
    27001 cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc
    27061 gcgtcagtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg ttcaggatcg
    27121 gctgccggca ggtcaatctt gttcagtact ggcaccactt cgagatccat ttccatggcg
    27181 gtgtagcagt ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac caccagcaat
    27241 gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac gtggcccggg
    27301 gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg
    27361 ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc gagaacctgc
    27421 gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc agacagcgtc
    27481 gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt cataaagtat
    27541 gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt ctgagcttaa
    27601 cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag gaaatgttca
    27661 taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa atgcaacaaa
    27721 gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg
    27781 gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac ttacgcgagt
    27841 agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat aatgctgcga
    27901 cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc accagcggcg
    27961 acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc aattccactt
    28021 tttgccccgg cactaacggt tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc
    28081 caagtttatt caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg ctgcatgaag
    28141 ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg gtagcccact
    28201 ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg ccgtttgcgg
    28261 cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac tgacggttct
    28321 gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa cgtttaccga
    28381 gaagctgaat aatccgtcag aatagagacg tgattcgata ggcatgttgt ccatcgtcgg
    28441 tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca
    28501 gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc
    28561 cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca cgcgaaattg
    28621 ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt tcgattcggt
    28681 gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc
    28741 gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt aaggagaaag
    28801 gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat cgccattaag
    28861 cgtgaacggt tcaagtcccg gttcaaaata gctgatttca tcgccgcgct gtaccacttc
    28921 ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga ggcgtgcatg
    28981 tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca gctcgtaatt
    29041 cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg gagtggccga
    29101 ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc aaagttgctt
    29161 cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc ggcttgctgg
    29221 gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc
    29281 tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg accattgttt
    29341 gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat catcggcagt
    29401 gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt atagtgctgg
    29461 acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt aagctgtgcc
    29521 gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg cgcaggctgg
    29581 gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc ggccatcacg
    29641 cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga gtcacggatt
    29701 aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc gttaagcagc
    29761 tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg catgcctaat
    29821 acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact ttgttatcaa
    29881 tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa tccatgatag
    29941 cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc atgcgcaaat
    30001 cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct gacaacatta
    30061 agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt tcagcttcaa
    30121 tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa tttttcgctg
    30181 tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg aacgaatcca
    30241 gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca cccgacggca
    30301 catagcggga aaccagactc gccactttat gctgatagcg cactaccagt aagttaaagg
    30361 ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac tgctcgctca
    30421 tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc actccattag
    30481 ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc cgtaacgcat
    30541 ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt ctacagaatc
    30601 tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt aacccaacgg
    30661 cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt ctcgccgccc
    30721 tgcatcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt attcgcacaa
    30781 catgatgcta tgctgaccaa accgtgttta gtaaattaaa caaagaaaat gaatactctc
    30841 cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact ttcactggcg
    30901 ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt aacggaaggt
    30961 tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga cagcattgac
    31021 tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca tgcagttgaa
    31081 tttgtcgcca gcaatgctcg ttcctgtgtg caatggttaa ttgaccaggg ggttttgttt
    31141 gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga aggaggacat
    31201 agtcaccgtc gtattcttca tgccgccgac gccaccggta gagaagtaca atccacgctg
    31261 gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa cgcagttgat
    31321 ctgattgtct ctgacaaaat tggcctgccg ggcacgcgac g
//
LOCUS       DAJVTY010000029        31139 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVTY010000029 DAJVTY010000000
VERSION     DAJVTY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31139
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.029"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(457..2148)
                     /gene="fruA"
                     /locus_tag="OQQ66_001208"
     CDS             complement(457..2148)
                     /gene="fruA"
                     /locus_tag="OQQ66_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1904211.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="OQQ66_001209"
     CDS             complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="OQQ66_001209"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1904212.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="OQQ66_001210"
     CDS             complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="OQQ66_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1904213.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4601..5782
                     /gene="setB"
                     /locus_tag="OQQ66_001211"
     CDS             4601..5782
                     /gene="setB"
                     /locus_tag="OQQ66_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1904214.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALTREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5779..6033)
                     /locus_tag="OQQ66_001212"
     CDS             complement(5779..6033)
                     /locus_tag="OQQ66_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1904215.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6188..6760
                     /gene="yeiP"
                     /locus_tag="OQQ66_001213"
     CDS             6188..6760
                     /gene="yeiP"
                     /locus_tag="OQQ66_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1904216.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6983..8449
                     /locus_tag="OQQ66_001214"
     CDS             6983..8449
                     /locus_tag="OQQ66_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1904217.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8567..9553
                     /gene="yeiR"
                     /locus_tag="OQQ66_001215"
     CDS             8567..9553
                     /gene="yeiR"
                     /locus_tag="OQQ66_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1904218.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9592..10305
                     /gene="lpxT"
                     /locus_tag="OQQ66_001216"
     CDS             9592..10305
                     /gene="lpxT"
                     /locus_tag="OQQ66_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1904219.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10717..11283
                     /gene="mepS"
                     /locus_tag="OQQ66_001217"
     CDS             10717..11283
                     /gene="mepS"
                     /locus_tag="OQQ66_001217"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1904220.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11464..13020
                     /locus_tag="OQQ66_001218"
     CDS             11464..13020
                     /locus_tag="OQQ66_001218"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1904221.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13102..14916
                     /locus_tag="OQQ66_001219"
     CDS             13102..14916
                     /locus_tag="OQQ66_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1904222.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14917..16011
                     /locus_tag="OQQ66_001220"
     CDS             14917..16011
                     /locus_tag="OQQ66_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1904223.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16011..17036
                     /locus_tag="OQQ66_001221"
     CDS             16011..17036
                     /locus_tag="OQQ66_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1904224.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17038..18627
                     /gene="yejF"
                     /locus_tag="OQQ66_001222"
     CDS             17038..18627
                     /gene="yejF"
                     /locus_tag="OQQ66_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1904225.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18631..18975)
                     /locus_tag="OQQ66_001223"
     CDS             complement(18631..18975)
                     /locus_tag="OQQ66_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904226.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="OQQ66_001224"
                     /pseudo
     CDS             complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="OQQ66_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="OQQ66_001225"
     CDS             complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="OQQ66_001225"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1904227.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21363..23123
                     /locus_tag="OQQ66_001226"
     CDS             21363..23123
                     /locus_tag="OQQ66_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1904228.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23248..23532
                     /gene="rplY"
                     /locus_tag="OQQ66_001227"
     CDS             23248..23532
                     /gene="rplY"
                     /locus_tag="OQQ66_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1904229.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLAIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="OQQ66_001228"
     CDS             complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="OQQ66_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1904230.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEECFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24860..25087
                     /locus_tag="OQQ66_001229"
     CDS             24860..25087
                     /locus_tag="OQQ66_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1904231.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25107..26867
                     /gene="pbgA"
                     /locus_tag="OQQ66_001230"
     CDS             25107..26867
                     /gene="pbgA"
                     /locus_tag="OQQ66_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1904232.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26942..27018
                     /locus_tag="OQQ66_001231"
     tRNA            26942..27018
                     /locus_tag="OQQ66_001231"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26976..26978,aa:Pro,seq:ggg)
     gene            27237..28478
                     /locus_tag="OQQ66_001232"
     CDS             27237..28478
                     /locus_tag="OQQ66_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1904233.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28974..29180
                     /locus_tag="OQQ66_001233"
     CDS             28974..29180
                     /locus_tag="OQQ66_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS1904234.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29195..29860
                     /locus_tag="OQQ66_001234"
     CDS             29195..29860
                     /locus_tag="OQQ66_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904235.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIINKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29886..30086
                     /locus_tag="OQQ66_001235"
     CDS             29886..30086
                     /locus_tag="OQQ66_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1904236.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30076..30297
                     /locus_tag="OQQ66_001236"
     CDS             30076..30297
                     /locus_tag="OQQ66_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904237.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30290..30523
                     /locus_tag="OQQ66_001237"
     CDS             30290..30523
                     /locus_tag="OQQ66_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904238.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30516..30749
                     /locus_tag="OQQ66_001238"
     CDS             30516..30749
                     /locus_tag="OQQ66_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904239.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30755..31054
                     /locus_tag="OQQ66_001239"
     CDS             30755..31054
                     /locus_tag="OQQ66_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1904240.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
BASE COUNT         7676 a         8033 c         7726 g         7704 t
ORIGIN      
        1 agcctataat tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc
       61 aaccctgtca atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat
      121 ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa
      181 aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag
      241 gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct
      301 tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat
      361 ttaaattcat aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac
      421 agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc
      481 cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
      541 tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc
      601 accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
      661 acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc
      721 ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt
      781 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
      841 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
      901 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
      961 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc
     1021 gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact
     1081 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag
     1141 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
     1201 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg
     1261 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
     1321 tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
     1381 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat
     1441 ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
     1501 ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc
     1561 cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc
     1621 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
     1681 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
     1741 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
     1801 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
     1861 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
     1921 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
     1981 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
     2041 gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata
     2101 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
     2161 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
     2221 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
     2281 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
     2341 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
     2401 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
     2461 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
     2521 cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
     2581 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
     2641 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
     2701 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg
     2761 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
     2821 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
     2881 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
     2941 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
     3001 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
     3061 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
     3121 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
     3181 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
     3241 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
     3301 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
     3361 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
     3421 caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
     3481 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
     3541 tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
     3601 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
     3661 attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc
     3721 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
     3781 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
     3841 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
     3901 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
     3961 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
     4021 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
     4081 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
     4141 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
     4201 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
     4261 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
     4321 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
     4381 tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg
     4441 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
     4501 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
     4561 gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt
     4621 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
     4681 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
     4741 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
     4801 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
     4861 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
     4921 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
     4981 tgcccttacc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
     5041 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
     5101 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
     5161 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
     5221 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
     5281 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
     5341 gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc
     5401 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
     5461 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat
     5521 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
     5581 gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa
     5641 cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg
     5701 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
     5761 actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat
     5821 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
     5881 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
     5941 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt
     6001 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
     6061 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
     6121 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
     6181 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
     6241 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
     6301 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
     6361 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
     6421 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
     6481 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
     6541 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
     6601 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
     6661 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
     6721 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
     6781 gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
     6841 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
     6901 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
     6961 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
     7021 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
     7081 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
     7141 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
     7201 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
     7261 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
     7321 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
     7381 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     7441 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     7501 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     7561 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg
     7621 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     7681 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     7741 ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg
     7801 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     7861 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca
     7921 tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa
     7981 tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt
     8041 aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg
     8101 tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac
     8161 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     8221 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     8281 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     8341 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     8401 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     8461 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     8521 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     8581 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     8641 taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga
     8701 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     8761 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     8821 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     8881 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     8941 acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     9001 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     9061 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     9121 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     9181 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     9241 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     9301 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     9361 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     9421 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     9481 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     9541 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     9601 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
     9661 attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat
     9721 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct
     9781 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
     9841 aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg
     9901 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg
     9961 tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga
    10021 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    10081 tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc
    10141 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    10201 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    10261 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    10321 atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
    10381 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    10441 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    10501 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
    10561 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    10621 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    10681 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    10741 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    10801 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    10861 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    10921 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    10981 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
    11041 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    11101 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    11161 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    11221 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc
    11281 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    11341 taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    11401 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    11461 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    11521 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    11581 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    11641 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    11701 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    11761 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    11821 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    11881 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    11941 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    12001 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    12061 cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc
    12121 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    12181 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    12241 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    12301 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    12361 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    12421 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    12481 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    12541 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    12601 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    12661 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    12721 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    12781 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    12841 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa
    12901 caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc
    12961 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    13021 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    13081 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc
    13141 accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    13201 ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    13261 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
    13321 ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac
    13381 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
    13441 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    13501 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    13561 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    13621 aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac
    13681 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    13741 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg
    13801 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    13861 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    13921 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    13981 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    14041 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    14101 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    14161 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    14221 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    14281 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg
    14341 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt
    14401 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    14461 caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc
    14521 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    14581 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    14641 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
    14701 gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    14761 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc
    14821 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    14881 gccaaactgc cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt
    14941 ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa
    15001 attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga
    15061 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    15121 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
    15181 atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg
    15241 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    15301 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    15361 ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc
    15421 tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg
    15481 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    15541 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    15601 acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa
    15661 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    15721 agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    15781 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    15841 ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    15901 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    15961 gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac
    16021 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    16081 cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttcagagctt atcgccaacg
    16141 ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    16201 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    16261 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    16321 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    16381 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    16441 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    16501 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    16561 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    16621 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    16681 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca
    16741 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    16801 ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct
    16861 tcggtctgcc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    16921 ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    16981 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    17041 acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta
    17101 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    17161 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    17221 ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc
    17281 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg
    17341 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    17401 caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt
    17461 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    17521 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    17581 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    17641 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    17701 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc
    17761 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    17821 tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    17881 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    17941 gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc
    18001 ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc
    18061 atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt
    18121 catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    18181 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    18241 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    18301 tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt
    18361 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    18421 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt
    18481 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    18541 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    18601 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    18661 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    18721 tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct
    18781 taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    18841 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    18901 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    18961 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca
    19021 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    19081 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    19141 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    19201 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    19261 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    19321 ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa
    19381 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    19441 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    19501 tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca
    19561 acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    19621 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc
    19681 ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa
    19741 agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    19801 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    19861 ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg
    19921 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    19981 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    20041 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    20101 cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag
    20161 ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg
    20221 ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc
    20281 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga
    20341 gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg
    20401 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    20461 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    20521 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    20581 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg
    20641 cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    20701 ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca
    20761 cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat
    20821 aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag
    20881 tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg
    20941 ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa
    21001 catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt
    21061 tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt
    21121 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    21181 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg
    21241 aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac
    21301 atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga
    21361 ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt
    21421 ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg
    21481 tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag
    21541 aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg
    21601 ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg
    21661 tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc
    21721 accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag
    21781 tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct
    21841 ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg
    21901 actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag
    21961 aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg
    22021 ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac
    22081 atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg
    22141 ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga
    22201 ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc
    22261 gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg
    22321 atcttatcgc cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc
    22381 gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc
    22441 ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg
    22501 tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg
    22561 acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg
    22621 gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg
    22681 aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg
    22741 atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt
    22801 ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag
    22861 acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg
    22921 agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg
    22981 ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca
    23041 tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc
    23101 gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata
    23161 gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt
    23221 agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag
    23281 cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac
    23341 ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa
    23401 gctaaagctg aattctacag cgaagttctg gccatcgttg ttgacggtaa agaaatcaaa
    23461 gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc
    23521 gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat
    23581 ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg
    23641 ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa
    23701 ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca
    23761 gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc
    23821 ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagcact cttccagttc
    23881 gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt
    23941 cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata
    24001 aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc
    24061 atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc
    24121 gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag
    24181 atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc
    24241 cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat
    24301 actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg
    24361 gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg
    24421 cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag
    24481 cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata
    24541 gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc
    24601 gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg
    24661 gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc
    24721 ggttaagcga cgcaataaaa tatggcggtc attttacaga aggcagcgta acgagaatga
    24781 tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc
    24841 aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca
    24901 attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct
    24961 gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg
    25021 ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc
    25081 gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag
    25141 tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg
    25201 tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt
    25261 attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga
    25321 tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca
    25381 ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc
    25441 atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg
    25501 cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt
    25561 ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc
    25621 cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg
    25681 atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga
    25741 tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc
    25801 gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc
    25861 gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact
    25921 actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt
    25981 tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg
    26041 gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta
    26101 ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc
    26161 cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg
    26221 acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc
    26281 gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat
    26341 ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca
    26401 atgcactgct tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg
    26461 gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc
    26521 cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata
    26581 ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat
    26641 attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg
    26701 ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta
    26761 aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt
    26821 tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc
    26881 agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag
    26941 tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa
    27001 atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca
    27061 cgaaaagccc gctaatcctt gattagcggg tttttttatg gtttttagtc accggacatc
    27121 accggattta taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc
    27181 gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg
    27241 cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg
    27301 aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac
    27361 tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat
    27421 cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa
    27481 tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa
    27541 gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga
    27601 atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt
    27661 tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac
    27721 ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg
    27781 taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta
    27841 aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg
    27901 agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat
    27961 ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca
    28021 acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg
    28081 ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc
    28141 accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag
    28201 ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg
    28261 ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac
    28321 ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa
    28381 aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa
    28441 ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa
    28501 ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa
    28561 acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc
    28621 cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc
    28681 atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt
    28741 aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg
    28801 gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca
    28861 tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
    28921 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
    28981 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
    29041 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
    29101 gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag
    29161 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
    29221 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa
    29281 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
    29341 agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag
    29401 ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt
    29461 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
    29521 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
    29581 gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc
    29641 gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg
    29701 tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattaaca agtggtcacc
    29761 tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc
    29821 aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag
    29881 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    29941 ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
    30001 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    30061 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    30121 acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa
    30181 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg
    30241 aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga
    30301 tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct
    30361 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    30421 atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc
    30481 gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa
    30541 tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat
    30601 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat
    30661 taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca
    30721 gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac
    30781 tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg
    30841 cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta
    30901 cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac
    30961 cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa
    31021 aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag
    31081 ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgc
//
LOCUS       DAJVTY010000030        30010 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVTY010000030 DAJVTY010000000
VERSION     DAJVTY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30010
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.030"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..832)
                     /locus_tag="OQQ66_001240"
                     /pseudo
     CDS             complement(1..832)
                     /locus_tag="OQQ66_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(807..1607)
                     /gene="otsB"
                     /locus_tag="OQQ66_001241"
                     /pseudo
     CDS             complement(807..1607)
                     /gene="otsB"
                     /locus_tag="OQQ66_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1774..2760)
                     /gene="araH"
                     /locus_tag="OQQ66_001242"
     CDS             complement(1774..2760)
                     /gene="araH"
                     /locus_tag="OQQ66_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1904241.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2775..4289)
                     /gene="araG"
                     /locus_tag="OQQ66_001243"
     CDS             complement(2775..4289)
                     /gene="araG"
                     /locus_tag="OQQ66_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1904242.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4359..5347)
                     /gene="araF"
                     /locus_tag="OQQ66_001244"
                     /pseudo
     CDS             complement(4359..5347)
                     /gene="araF"
                     /locus_tag="OQQ66_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6061..6564
                     /locus_tag="OQQ66_001245"
     CDS             6061..6564
                     /locus_tag="OQQ66_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1904243.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6643..6894)
                     /locus_tag="OQQ66_001246"
     CDS             complement(6643..6894)
                     /locus_tag="OQQ66_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1904244.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7009..7095)
                     /gene="azuC"
                     /locus_tag="OQQ66_001247"
     CDS             complement(7009..7095)
                     /gene="azuC"
                     /locus_tag="OQQ66_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1904245.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7358..7681
                     /locus_tag="OQQ66_001248"
     CDS             7358..7681
                     /locus_tag="OQQ66_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1904246.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7852..8349
                     /gene="ftnA"
                     /locus_tag="OQQ66_001249"
     CDS             7852..8349
                     /gene="ftnA"
                     /locus_tag="OQQ66_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1904247.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8387..8626)
                     /locus_tag="OQQ66_001250"
     CDS             complement(8387..8626)
                     /locus_tag="OQQ66_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1904248.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8817..10028
                     /gene="tyrP"
                     /locus_tag="OQQ66_001251"
     CDS             8817..10028
                     /gene="tyrP"
                     /locus_tag="OQQ66_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1904249.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLQANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10090..10755)
                     /gene="yecA"
                     /locus_tag="OQQ66_001252"
     CDS             complement(10090..10755)
                     /gene="yecA"
                     /locus_tag="OQQ66_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1904250.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10951..11037)
                     /locus_tag="OQQ66_001253"
     tRNA            complement(10951..11037)
                     /locus_tag="OQQ66_001253"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11001..11003),aa:Leu,seq:taa)
     gene            complement(11050..11123)
                     /locus_tag="OQQ66_001254"
     tRNA            complement(11050..11123)
                     /locus_tag="OQQ66_001254"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11089..11091),aa:Cys,seq:gca)
     gene            complement(11178..11253)
                     /locus_tag="OQQ66_001255"
     tRNA            complement(11178..11253)
                     /locus_tag="OQQ66_001255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11218..11220),aa:Gly,seq:gcc)
     gene            complement(11405..11953)
                     /gene="pgsA"
                     /locus_tag="OQQ66_001256"
     CDS             complement(11405..11953)
                     /gene="pgsA"
                     /locus_tag="OQQ66_001256"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1904251.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(12010..13842)
                     /gene="uvrC"
                     /locus_tag="OQQ66_001257"
     CDS             complement(12010..13842)
                     /gene="uvrC"
                     /locus_tag="OQQ66_001257"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1904252.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13839..14495)
                     /gene="uvrY"
                     /locus_tag="OQQ66_001258"
     CDS             complement(13839..14495)
                     /gene="uvrY"
                     /locus_tag="OQQ66_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1904253.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14790..14966
                     /locus_tag="OQQ66_001259"
     CDS             14790..14966
                     /locus_tag="OQQ66_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904254.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14953..15177
                     /locus_tag="OQQ66_001260"
     CDS             14953..15177
                     /locus_tag="OQQ66_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1904255.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15244..15992)
                     /gene="sdiA"
                     /locus_tag="OQQ66_001261"
                     /pseudo
     CDS             complement(15244..15992)
                     /gene="sdiA"
                     /locus_tag="OQQ66_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(16222..16974)
                     /gene="yecC"
                     /locus_tag="OQQ66_001262"
     CDS             complement(16222..16974)
                     /gene="yecC"
                     /locus_tag="OQQ66_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1904256.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(16971..17639)
                     /gene="tcyL"
                     /locus_tag="OQQ66_001263"
     CDS             complement(16971..17639)
                     /gene="tcyL"
                     /locus_tag="OQQ66_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1904257.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17654..18640)
                     /gene="dcyD"
                     /locus_tag="OQQ66_001264"
     CDS             complement(17654..18640)
                     /gene="dcyD"
                     /locus_tag="OQQ66_001264"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1904258.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDTLTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18745..19545)
                     /gene="tcyJ"
                     /locus_tag="OQQ66_001265"
     CDS             complement(18745..19545)
                     /gene="tcyJ"
                     /locus_tag="OQQ66_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1904259.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19633..20184)
                     /gene="fliZ"
                     /locus_tag="OQQ66_001266"
     CDS             complement(19633..20184)
                     /gene="fliZ"
                     /locus_tag="OQQ66_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1904260.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20230..20949)
                     /gene="fliA"
                     /locus_tag="OQQ66_001267"
     CDS             complement(20230..20949)
                     /gene="fliA"
                     /locus_tag="OQQ66_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1904261.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(21293..22975)
                     /locus_tag="OQQ66_001268"
     CDS             complement(21293..22975)
                     /locus_tag="OQQ66_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1904262.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSGAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQ"
     gene            23241..24647
                     /gene="fliD"
                     /locus_tag="OQQ66_001269"
     CDS             23241..24647
                     /gene="fliD"
                     /locus_tag="OQQ66_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1904263.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24672..25082
                     /gene="fliS"
                     /locus_tag="OQQ66_001270"
     CDS             24672..25082
                     /gene="fliS"
                     /locus_tag="OQQ66_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1904264.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            25082..25448
                     /gene="fliT"
                     /locus_tag="OQQ66_001271"
                     /pseudo
     CDS             25082..25448
                     /gene="fliT"
                     /locus_tag="OQQ66_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            25526..27013
                     /gene="amyA"
                     /locus_tag="OQQ66_001272"
     CDS             25526..27013
                     /gene="amyA"
                     /locus_tag="OQQ66_001272"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1904265.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(27047..27460)
                     /locus_tag="OQQ66_001273"
     CDS             complement(27047..27460)
                     /locus_tag="OQQ66_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1904266.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27647..28852
                     /gene="yedE"
                     /locus_tag="OQQ66_001274"
     CDS             27647..28852
                     /gene="yedE"
                     /locus_tag="OQQ66_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1904267.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            28849..29082
                     /gene="yedF"
                     /locus_tag="OQQ66_001275"
     CDS             28849..29082
                     /gene="yedF"
                     /locus_tag="OQQ66_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1904268.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            29189..29860
                     /locus_tag="OQQ66_001276"
     CDS             29189..29860
                     /locus_tag="OQQ66_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS1904269.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
BASE COUNT         7462 a         7715 c         7194 g         7639 t
ORIGIN      
        1 agcaacgctt cataggcgag aaaacgctct ggcaaacctt tggaataatc cagccgttcg
       61 acagaaaaga tattttgtac gtttttcagt tccgctttaa gttgcgccag ttttggcggc
      121 agtggcccgg cagcctgttt ggctatttct ttcggttcaa tgccgatcgg gtagacttct
      181 gttcgaaatg ctttgcccca ggctgtatgg ctttttgcgc tacgtgtcgt gacgcgggtc
      241 aggttagaaa gacaatccag gaacgccaga cgatcgtttt ctgtctggaa acccagcaaa
      301 tcataatcac aaagctgttc aagcaaggtg tcatatgtcg gcagcgcgtt gaagatttcc
      361 ggtgtcggga aaggaatatg cagaaagaaa ccaatgcgat tattcacccc acgtttgcgt
      421 aattcatgcg caaagggcaa caggtgataa tcgtggatcc agataatgtc atcgtcttgc
      481 aacagcggca gtaatttatc tgccagcaac gcatttacgc gtagatagcc gtcccaggca
      541 ggacgctgaa attgcatcag atcgagccga taatgaaaag cgggccagag aacggcattg
      601 gagaattggt tgtagtattc gtcaaggtcc tgttcgctga ggttaaaaga ggcccacgta
      661 atgttacctt ttttagcggc tgatcctcat tccctgtttc accactccag ccaaaccaca
      721 gtccgcctgt ggctttcagt gcccccagta tgccaacggc aaggccaccg gcactggcgg
      781 cgtgctcgtc tggtggtgca atccggttag atactacgac taaacgactc atagtcatca
      841 ctcctgttat tttctctttt ttgttgtaat gcggtggtta tcatttcaag cctgctccag
      901 acatccggca cacccgccag tcgccatgat gcctgagttg cacctgtgcc aatttttact
      961 gacattccgc ccagtcggtt aacgactgcg aagccagatt catcggttaa atcatcgccc
     1021 agaaatacgg gcgttcgccc gataaaggga gcttcctgca taaaagctgc aattgcctca
     1081 cctttactgg tacctctcgg tttgatctcg acaacacact ttccctgctg taacgccatt
     1141 tgtggccaga tctgagtaat acgttacgct aatgtcatta atgcgtcttc atgctgcgga
     1201 gcctgacgat aatgcagcgc aaaagccatc cctttcgcct ccagctccgc gccgggatac
     1261 tgagcgataa ctgtatgcag ttgcacgcta atatcacgcg caatcgcatc cggcagatga
     1321 acgatatgcg ttttaccatt gatgtcacgg cgctccgccc catgcacgcc cgctaacggg
     1381 aagcgataag gttttgccag tgcgtcaagc tccaccattg agcgccctga tatcaatgcc
     1441 aatgcaccat cacttgcggt tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg
     1501 acgacctgat cgggatgcgg tttgatttcc gccagcgttc catcgagatc aaaaaaccag
     1561 gcatatttcg cggatagttc aggggtttcg gttaacggtt ctgtcacccg gttctcctac
     1621 ttctcttttc ttattgtttt gcgcagaacg cgctttacag acatgtaagt atagacagtg
     1681 tgacgggggt cgccatttgc aacgaagaac agccaaccgt tatggcggtt ggctgtggtg
     1741 ggaaaaaacg ctaaattgtt gcagaaaaaa gcatcagaca gtgcgtttcg ctttttgctt
     1801 gtaacggtcg aagatcaccg ctgccagcag gattaagccg cgaaccacgt actgcgcgaa
     1861 aggagaaata ttaagcaggt tcatggcgtt ttccacggtg cctaaaatta agatacccgc
     1921 caccacatat gagatttttc cgatgcctcc tttcagagaa acgccaccta aaacgcaggc
     1981 ggagataaca atcagctcgt aaccaatcga cgtcattggc tgcccactgg tcatacgtga
     2041 agccagaata attccggcta tcgcagatac caggccagag agaacaaaga taataatttt
     2101 ggtgcgaaca accggtacac ccgccagacg cgcagcctct tcgttcccgc caatcgccag
     2161 ggtgttacga ccaaaggtgg ttttattcag caacaaacca aagataatca gacacgcgac
     2221 ggtgagccag attggcgcag gcagaccgaa ccagttagcg taaccaaggg caaagaagct
     2281 ttcatcttcg ataccgaccg ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat
     2341 ctgcatcgtt gccagtgttg tgatcagagc atttattttc agtttggcga taacaaagcc
     2401 attgaccagg ccacagagaa tgccaagcag caaccccgct gccacgccaa tccacaggct
     2461 ttcagtcagg ttgataacca ccgccgtggt gacacccgca caggcaatta cggaggcgac
     2521 agaaaggtca aagtcaccgg aagcaaggca gaacaacatg ccacacgcca ccatccccga
     2581 catggaaatt gccaggccca accctttcat attaatgaag gtggcaaaat ttgggacaaa
     2641 aatggcacag gcgataaaga gcaccgcaaa caccaccagc atgccgtact gatcccagat
     2701 acgcccgaag ctgaatgacg acttaggtgc gccagaaccc gatgtagaaa cagaagacat
     2761 catactctcc ttactcaggc aactgcctgg ctgactttag gcatcgcaag gctcagtgcc
     2821 tgacgctcat ctgcctgctc gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc
     2881 acaatccggt cggcaacgcc gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc
     2941 acaccctgcg ccgccagcgc ataaattacg ttatatattt cgtgcttagc accaacatca
     3001 atgccgcgcg taggttcatc cagcaaaatg accttcatct cttccgataa ccagcggccc
     3061 agaatggctt tttgctgatt tccgcctgag agattcatga tcagttgctc agcgcccggc
     3121 gttttgatgt tgagcgaacg aatgtgctga tcggcattgt tttcttccca accgttgttg
     3181 attacacaac cgccgagcac atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg
     3241 gagtgcacgg gaataatgcc ttccgctttg cgatcttccg ggcagagcat catgcctgcg
     3301 gcaatggcgt ggctcggttt acgaatatcg atcggctgtt ggtcgatata aacctgaccg
     3361 gcggtgattt gcgtcccgcc aaacaagcct ttcattaatt cgctacgccc cgcccctacc
     3421 agaccaaaca gcccaacaat ttcaccactg cgaaccgcca gacttattgg cgtacgcacg
     3481 cctggtgctt tcacagcatc aagacgcagg cgctcctcgc cataactacg aggttgccag
     3541 ccgtagatat cgccaatgtc gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc
     3601 tgctgcatat cggtaaaggt tttgacataa cgtccatctt taaagacagt gatggcatcg
     3661 ctgagggcaa atatttcttc catacggtga gaaacgtata agatcacccg cccctctttt
     3721 cgcagttcac gaataacgcg gaaaagattg tcgatttcgc gggcagaaag ggagctggtt
     3781 ggctcatcaa aggcgataat tttagcgtta cgcgccagcg ccttggcgat ttcaaccatc
     3841 tgccactgac caatggagag atatttcagc ggcgtgtccg ggtcaatatc cataccaaga
     3901 tgtttaagtt gtaaacccgc ctcataattc agcaatgagc gattcacaat gccgccttta
     3961 tgcggcagct ggccgagata gatgttttcc gcgacggtca tttccggcac gagatgcagt
     4021 tcctggtaaa taatcgccac acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt
     4081 tcctgcccat taatcactac agaacccgtg gttggcgcat agttgccgct gaggattttt
     4141 aagagagttg attttcctgc gccattttca cccatcaacg catgaacctg accggcatag
     4201 cagtcaaaac taatatccgt cagcgcctta acgccgggaa acgttttacc gatgccgcga
     4261 aatgagagat acggggtaga ctgttgcata acgtctccgt gaatcactgg tcgttcacac
     4321 cccctctgca tcatgcagag gggaattttt ccggcaaatt acttaccgcc taaacctttt
     4381 ttctccagtt cttctttaaa gttgtcacgc gtgatcagca ccacgtcggt aacttcggta
     4441 aattttggcg gttcaacgtc ttttgctacc cagttgtaaa gcatttcgct ggatttatag
     4501 ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc cggttgcctg tactttagac
     4561 agttcgctca ccgcatccac accgttaatg ccaatgccga taatatcggc agctttaaag
     4621 ccctgacctt ccgtcgcgcg tacgccgccc agcacggtgc tgtcgttcat accgacgatc
     4681 agccaatgtt taacttcagg atgttgaacc agcattgagt tggcagcgtc aaatgccccc
     4741 gggatgtcgt tagatttggt aggtacctga taaatttgtt tttccgggaa tccggccgct
     4801 ttcagcgcat ccatagatcc cgtagtacgg cggcgggcag tatccagttc gttggcggta
     4861 atcaccatca ccgcgctttc tttgacatcc cagccacgtt tctgcatctc tttatacagt
     4921 tcctgaccct gacgttcgcc aattttagtc gcgccatcat caccagcgga acggtatcca
     4981 ttggcttacc tttggcgtta acaaactgat catccacggc aatgactttc atatcgtagc
     5041 cacgcgcttt cgcgacgatg gcagagccga gtttggggtc cggagtacaa ataacgaaac
     5101 cttttgcgcc actggcagcc aggctgtcga tcgcgttcaa tgttttttcg ccatccggca
     5161 cggcaatctt aataacctca aaccctaaat ccttcccggc tttatcggca aacttccatt
     5221 cggtctggaa gcacggctct tccggttgct tcaccagaaa accgagcttc aggttctccg
     5281 ccatagcgga ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt
     5341 tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc
     5401 gttaaattaa tcggaaaaca aagcattacc ttttaactaa aagataagtg accgtgttga
     5461 catagtttta gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca
     5521 gtgagaacgt gcataaattt agcgggaaaa gacataaggg aaagccaatt tgtcagacaa
     5581 attgtcgaat gcacagcaga ttaatccata agattagctt ggaaatcctt gttgtctttg
     5641 gtacccatgc gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag
     5701 tttttgaaat ggtgtaataa ccgcaactca aagatgtgga aaatgcacgt cattcatttc
     5761 gccattaatt atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc
     5821 aaaaatgaaa aaacgtttta taaaatcatc atttcatcaa tgaaatgtaa aaatatataa
     5881 acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt tcagatcatc
     5941 gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt ctgcaaacag
     6001 taattatggt gttttgattt atcttgcacc tctccacttc tggatataag gatattaggt
     6061 atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga gttttacgca
     6121 tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa cggcaccgcc
     6181 actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat gtttaacttt
     6241 atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg tgaaaaactg
     6301 aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg ttctagtacg
     6361 ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt caatttcctg
     6421 cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat tcttgatgaa
     6481 gtgcgcagtg cgaaacttgc tggtatgtgc ccagtgcaga ccgaccaaca tgttctgaat
     6541 gtcgtgtcgc atcagctgca ttgatcatac atcggcgcta atgcattgcg ccgatgaagt
     6601 ttttgagaaa ccgctgcctc atctttttga agcagcggtt ttttaatggg attcgccttg
     6661 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     6721 gtctgcggcg gcgggatggt tactaaagtc gatattcttc agctgactgg acattttttc
     6781 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     6841 gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     6901 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     6961 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     7021 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     7081 tttgcgcagt ttcatcgcgg cactccgacc atttgttata catgtgttat tttctttgtg
     7141 cttataatat gacaaccatc acaaaaatca atctttatgt gatgcaaatc acacaaatac
     7201 ccccttaatg ttataaaaat gataatcaaa aaacagcccc cccatttctg acacctacag
     7261 atggcaagaa atagcgcctg ccaggcgtct tttccggtca ttgtcgcagc gctgtaacgc
     7321 gtaaaatagt gctttctttt actcttctgg ctggaccatg agacttctga ttctgactct
     7381 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     7441 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     7501 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     7561 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     7621 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     7681 aatattttaa tcagcgaggg gatcttcgct gattaaaaaa atagcggtaa tgcgttttat
     7741 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     7801 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     7861 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     7921 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     7981 cgccgtcacg cccaagaaga gatgacgcat atgcagcatc tgtttgatta cctgactgat
     8041 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     8101 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     8161 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcaatggtat
     8221 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
     8281 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
     8341 caaaactaat actctccggc ggcaggttta ctgccgccgt ttcaaatcag tgacggcaaa
     8401 tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct tttgctgcca
     8461 gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga aaacgtgcct
     8521 gttcaccaaa acgcgcttta attgctgctt ccagactggc atgcgtatat tgctcgcctg
     8581 attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt cctcctcaaa
     8641 aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagagcag aactgaatgc
     8701 tttttattgt acatttatat ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta
     8761 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
     8821 aaaacagaac tctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
     8881 tggcaatgcc gctggctgcg gcaggtgttg gttttagcgt cacgttaatc ttgttgattg
     8941 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc
     9001 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc
     9061 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg
     9121 ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
     9181 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg
     9241 tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg
     9301 tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc
     9361 tggctctgtc agcaatcccg gtgattttta cctcatttgg ttttcacggt agcgtgccga
     9421 gtattgtcag ctatatggat ggcaacgttc gtaagctacg cagggtgttt ataaccggta
     9481 gtgcgatccc cctggtggca tatattttct ggcaggtggc aacgcttggc agcattgatt
     9541 caacaacctt tatgggattg caggctaatc atgctggatt aaacgggctg ttacaggcgt
     9601 tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag
     9661 ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttat
     9721 ttcagcgttc aaataccgtt ggtggacggt tgcaaactgg tgcaattacc tttctgccgc
     9781 cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
     9841 tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa agcagaaagc
     9901 acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc
     9961 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag
    10021 tgaggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa
    10081 tttggcctct taatgcaggc agcactgctt aaatttctta ccactaccgc acgggcaagg
    10141 atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg
    10201 atgcgccatc cagtacgcat gtagatcaag cgccgccagt cgaatggcat ctacgctctc
    10261 ttcaaacgct tctggcgaca tcttttctac ccgctcgaag ttttcctcag taccgtgcag
    10321 cgcaatcgcc tccagcgctg gttttaacga atcgggcaac gttgaccagt cagaaagtgc
    10381 cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac
    10441 ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt
    10501 atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca
    10561 gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttccg gttcaatctc
    10621 ttgcggagaa ctcaacaccg ccgtcaataa atcgtccagc tccgccacat caaggatggc
    10681 gtggttagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa
    10741 cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat
    10801 gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa attaaggagg
    10861 gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt
    10921 attgctttga ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac
    10981 agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg
    11041 ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta
    11101 cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac
    11161 gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc
    11221 aaggtcgtgc tctaccaact gagctattcc cgcattcatt aagcaatcag ttaatcactt
    11281 gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg
    11341 gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa
    11401 acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca
    11461 gagtcagcac cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg
    11521 gacgccacag cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc
    11581 aggagacagc cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa
    11641 taataatttc acgggcgatc atcgttgccg ccggtagggt cacccaccag ctgtgataat
    11701 gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgctaca gggtcaagga
    11761 aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag
    11821 tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat
    11881 aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga
    11941 tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct
    12001 acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc
    12061 cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata
    12121 tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca gggaactggt
    12181 atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc
    12241 gcgaatatgc tggataacat gcagcgcggg tgaatctggc ggcaaactaa atccctcatc
    12301 ttccggctca aagaacagcg tttccagtcc agccttacga tctgctcctt tggcaacgcc
    12361 aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc
    12421 ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc
    12481 aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg
    12541 cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa
    12601 caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat
    12661 ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt
    12721 agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag
    12781 ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc
    12841 tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc
    12901 cggtaaggtg cgcatctggc tgccttgtaa atagaactga cctacgaagg tttctaccac
    12961 ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt
    13021 gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat
    13081 aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc
    13141 ctgaatttgg tcgcgaatac gtgccgcttc ttcaaactcc agattctgac tggcagtttc
    13201 catacggcta atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac
    13261 atactcgacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag
    13321 acagcgccct atctgatatt gcagacacgg acgcgagcga ttgcgataaa cactattttc
    13381 gcactggcga atggggaaaa tcttttgcag tagcgccaga gtttcacgta cggcatagcc
    13441 attcgggaac gggccgaaat attcaccttt ggcatgcttc gcaccacgat gcatcgccag
    13501 acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa
    13561 aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt
    13621 ttctgtatga gtaaccgtta catcaatttg ctgaatctgg gcgaccagcg cttcggtttt
    13681 gcgcgaagcg aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt
    13741 gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt
    13801 tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc
    13861 gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt
    13921 agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt
    13981 tctgagatct cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt
    14041 tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt
    14101 tgttgagcga tgtcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc
    14161 acgacttcct gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact
    14221 ttcgctggta aagggttttc tgtatgaacg gtaagcatga tgattttgac atcagctgtg
    14281 gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt
    14341 agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc
    14401 tcaccgacga cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc
    14461 agttcgtggt catcaacaag tagaacgttg atcaaagaaa tatctccaga aatagggata
    14521 acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta
    14581 aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg
    14641 tcacgcccgt caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg
    14701 acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat
    14761 taacccgaac aatacgggca aaaacattca tgataaaaat atttattggt cattatatta
    14821 acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta tttttagcaa
    14881 agcttatggt atactccgcc gccttaacat ttttcaccgc aaattttcat tgcaacatga
    14941 cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca agaactggca
    15001 aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat gggacaagct
    15061 gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga agacgaaacc
    15121 caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg tcagtaatat
    15181 aaaaccggct gagagcgtgc ctttcagccg gtttttgcat ctggcacgca gaaagaaaag
    15241 cgatcaaatt aagccagtag cggccgcgta acaggccacc tgggtcttat ttggtgcatt
    15301 gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga ttgacaaaat
    15361 catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcagaattt ctttttcgcg
    15421 cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat
    15481 cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat
    15541 ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccagc gcccgatttg gcagcattaa
    15601 atactgagtg acaccgcggc gtaaaccatg tgcgtgcgcg gcttcccata atggctgtgc
    15661 ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt cagggttcag
    15721 cacaggatca attgcgagaa aattagcaca ggatcaattg cgagaaaatt ttttgcctga
    15781 taataactaa cccacgactc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat
    15841 ggtaccgggt ggcgaacaca taacgaatag taatcgtact ccagctgctg agcctgaagc
    15901 tctatttcat ggtagacctc ttctgcgacc tccatcctct gaaaacgcaa cagcatcgtg
    15961 cgacgccaac tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag
    16021 tatcatttat aatgataatt caaactatca cataaataag atttatatat aatttatatt
    16081 attcaggcaa tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat
    16141 gctgatttgt taagcagttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc
    16201 gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact gtcgggtgcg
    16261 aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgccccag
    16321 gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat
    16381 caccatcgtg cgcttttcct gcgccagctg gcggattgtg ttcaggactt cgcccaccag
    16441 ctctggatcc agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag
    16501 cgcacgcgca atcgctacac gctgctgttg accgccagac aaacgacgtg gatagctggt
    16561 ttctttaccg gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc
    16621 tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg
    16681 cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag
    16741 cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt
    16801 tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc
    16861 actcggaccg ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag
    16921 caccgtctga ccttggaatt ttttcaccag gttcttaact tcaatggcac tcatttgggt
    16981 tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga taacacagtc
    17041 gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc
    17101 gaggtaatca actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac
    17161 gtatctttta ccagactgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc
    17221 tgtggcaaaa tcgcgcgacg catggtctgc cacggcgtca taccaatact ggcagcggct
    17281 tcccactgac ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc ggcataggcg
    17341 gcagtgttca gcgacagacc aatcattgct gacagaatgg gatctaattc gataccaaac
    17401 tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata
    17461 gagatataaa aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg
    17521 agaataaacc cgagtagtaa gccaaaaaac atgccgccga tactgagttg cagggtatac
    17581 ccagcccctt tcaacaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg
    17641 agcgttcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgcg ccgccggtat
    17701 gaataaacag aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc
    17761 ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccaga cgcgccagca
    17821 atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat
    17881 cccagagtaa aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta
    17941 ggttaaccac tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt
    18001 cgctttcagg catcaggtgt tccagcccaa cagccaatcc ggcgtgagtt ccggcactgc
    18061 ccgatgcgac caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca
    18121 gcgcactctc cacataaccc agcgcgccca gagcattaga accgccaacc ggaatgacat
    18181 acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat
    18241 cggtcagtgt gtcgcacatt tcaatctggg tattgaacag attcagcaac aaacgattgc
    18301 cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt
    18361 gcaggccgag tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgcgc
    18421 cggcagtaat cagcgtatcg gcaccttcgc gcagggcatc tgccgcgaga aattccagct
    18481 tacgtaattt attgccgccc attgccatgg gtgtgacgtc atcccgtttg atgaaaattt
    18541 cccgtccaag ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa
    18601 actccagccg tggaaaacgg gttaaattat gcagtggcat aatagcctcc gatgtgtgtt
    18661 gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag
    18721 cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga
    18781 aagggcttgc agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag
    18841 caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc
    18901 ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac
    18961 gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata
    19021 ggtacgcaca tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag
    19081 accgacaccc acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc
    19141 ttttttcacc agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt
    19201 cttgcgctca tcagaaatgg tgacctgatt aatcaccaca tcaatacgtt tagagtccag
    19261 cgacgctagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
    19321 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
    19381 actgaacggc ggataagttc cttccagccc taccagtagc gtaccgcgct ctttgacttt
    19441 attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc
    19501 catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt
    19561 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
    19621 tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc
    19681 ggtacacgtt tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt
    19741 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc
    19801 gagggaaata ttttgctcat gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc
    19861 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
    19921 atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc
    19981 ccatgactgt tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat
    20041 ctcgatttta ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg
    20101 gcaatgcgcg caatgggcct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
    20161 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat
    20221 ttactgacgt tataacttac ccagtttagt gcttaaccgt ttaatggcct ggctgtgtaa
    20281 ctgactgacc cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc
    20341 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc
    20401 catcacccgc tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
    20461 atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
    20521 aagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa
    20581 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
    20641 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
    20701 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
    20761 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
    20821 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
    20881 cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag
    20941 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
    21001 agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa
    21061 attccatgca gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattgcgctt
    21121 tatgttgccg gatgcggcgt aaacgcctta tccggcctac aaaaatgtgc aaattcaata
    21181 aattgcaatt caccttgtag gcctgataag tgcagcgcat caggcaattt ggcgttgccg
    21241 tcagtctcag ttaatcaggt tacgacgatt aaccctgcag cagagacaga acctactgcg
    21301 gtacctggtt ggcttttgcc agcacggagt taccggcctg ctggatgatc tgcgctttcg
    21361 acatgttgga cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gctccagaca
    21421 ggttggtagt ggtgttgttc aggttggtga ctgcagaatc cagacggttc tgaatagcac
    21481 ccagggatga acggaatttg tcgatggagc tgatagcttc atccagggca gccagaggat
    21541 ctttggttgc agtgcctgca ctggtggttt cagtggtcag tttgcctgta gagttaacat
    21601 aagcctgtgc cccaacagta gctgcaaatt taccagttgc acctgcgcca tttgaagcta
    21661 cagttactgt accggtatcc ttatcgacgt tgtagttgat agtgtaagat gcggttgtgg
    21721 tgagctcttt gtctgcatca gtataggtgt cattcgaatt accagcacca ttagtattcg
    21781 caccaccgaa ggacgccgtt gcagactgaa tacctgtatt atacttaatt gtcgcaccag
    21841 taactgctgt gttagccgtt gcagcactct gcactgtgct cagtacagta tcagcgctta
    21901 cagttttggt gtaggttacg ccagaagttg ccgctgcacc aaagttataa gttgtgccag
    21961 acaactggat agaaccagtt gtcgctaagg tactaccgct agcaaacaga tcggacaatt
    22021 tcgctgaaga tgcaatgccg gcgttgtttg tagataggtt accagtggcg tccaggtacg
    22081 cggctttacc accgatggtc acatcgccat tagcatctac atcaaacttc acatcaccag
    22141 tactacgagt ataaacacct gatgcagtag tgccagccgc tggtttcaga gtattcgcaa
    22201 agttaacaac atcgccatct gcaactgttg cttgagtggt gaagttccct ttagccgcat
    22261 cataagtata gatcgcagca ctcgagccag tagtagtaac tgtatctccg gttttcaggc
    22321 gagacagtgc atcgctagcg ctgagtgctg tattgtttgt ggtcacagca taaggacctg
    22381 ttcccgttgc accagcagcg gtcagatcgc tgactgtagc agctttgttc gcaatagtgc
    22441 ctttgccatt cacgttaaaa ccattcagcc ccagcgtatc tgagtcaatt ttcttcagat
    22501 caatcgtgat agtctggccg tcattcgcac caacctgaat tttcattgaa ccgtctttcg
    22561 ccagtacgtt cacgccgttg aactgggtct ggccagatac gcggtcaatt tcgtccagac
    22621 gggatttgat ttcgtcctga atggagtcca gatccgaatc ggagttagtc ccggtagaag
    22681 cctgaaccgt cagttcaaga atacgctgta agttgttgtt gatttcggac agcgcgcctt
    22741 cagtggtctg cgcaacggag ataccgtcgt tggcgttacg ggccgcctga gtcaggcctt
    22801 taatattaga agtaaaacgg ttagcaatcg cctgacctgc ggcgtcatcc ttcgcgctgt
    22861 taatacgcaa gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt
    22921 tgatattatt ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc
    22981 gttatcctat attgcaagtc gttgattacg tattgggttt ccacccgtcg gctcaatcgc
    23041 cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca
    23101 ttttttgtta gtcgccgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa
    23161 caattaaacg taaactttgc gcaattcagg ccaataaccc cggtattcgt tttacgtgtc
    23221 gaaagataaa aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg
    23281 atttaagttc aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt
    23341 caaatcagca atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc
    23401 tgacgacttt ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca
    23461 gcactaccag cagcaccacc gcgttcagtg ccaccactgc gggtaacgcc atcgccggga
    23521 aatacaccat cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca
    23581 gagatgatac gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg
    23641 gcgacaaaga tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc
    23701 gtgatgctat caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg
    23761 gtgaatatcg tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct
    23821 cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca
    23881 acggtatgga ggtctcggtc gccgccaaga atgcgcagct gacggtcaac aacgtcgcca
    23941 ttgagaacag cagcaacacc atcagcgacg cgctggaaaa catcaccctg aacctgaacg
    24001 atgtcaccac gggcaaccag acgctaacca tcactcagga cacctccaaa gcgcaaacgg
    24061 cgattaaaga ctgggtgaat gcctataact cgttaataga taccttcagt agcctgacca
    24121 aatacaccgc cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg
    24181 gcgactccac gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca
    24241 gttcttccag ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca
    24301 aactggaact ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag
    24361 gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac gaccaccatc ggcagcaacc
    24421 tgaccagttg gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaga
    24481 ccctgaataa gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg
    24541 cgcgctacaa aaaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca
    24601 gcagctactt aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg
    24661 ggagacaggt catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa
    24721 gcgccgtaat gagcgccagc cagcagcagc tggtcaccat gctatttgat ggagtgctga
    24781 gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct
    24841 ctttgtcaaa agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga
    24901 gcaaagacga actaacccaa aacttgattg ctctttatag ctatatggtc aggcgcttgc
    24961 tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga agtggaagca ttaatgcgca
    25021 atattgccga tgcctggaaa gagtcgttac tctccccttc tttgattcag gacccagtct
    25081 gatgaaccat gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct
    25141 catgttacgc ctggcaacgg aagaacaatg ggacgaactc atcgccagcg aaatggcgta
    25201 tgtgaatgcg gtgcaggagt atagcacacc tgactgaaga ggttgccccg tccaccacga
    25261 tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc aaggtaaagc
    25321 agttattaca gattcgtatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
    25381 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caagataact
    25441 tctcgtgaat cttaacgagc taatggttcg cgaataatcc gattacggct acgcttctaa
    25501 tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
    25561 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg acggttttaa
    25621 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcgggta
    25681 ttcggtcggc tacgactcct ataatttatt tgatttaggc gagtttgatc agaaaggcag
    25741 catccctact aaatatggcg ataaagtaca attgctggcc gccattgacg ccctgaaacg
    25801 taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
    25861 agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt acgcaaattg atgaagaaat
    25921 cattgagtgc gaaggctgga cgcgttacac cttccccgcc cgtgccgggc aatactcaca
    25981 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga
    26041 tggcatattt aaaattgtta acgactacac cggcgaaggc tggaacgatc aggttgatga
    26101 tgaattaggt aatttcgatt atctgatggg cgagaatatc gattttcgca atcatgccgt
    26161 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt
    26221 tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga tcgaacacgt
    26281 acaggaagtt gcgccaaagc cgctgtttat tgtggcggag tactggtcgc atgaagtcga
    26341 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg atgcgccgct
    26401 gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac tacgacatga cgcagatttt
    26461 cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg ccaatcacga
    26521 cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg tggtttaaac cgctggcgta
    26581 tgccttaatt ttgttgcggg aaaatggcgt tccttcggta ttctatccgg acctctacgg
    26641 tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    26701 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt
    26761 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    26821 ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattcatc tgggagagaa
    26881 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg taataaccga
    26941 cgaacacggc gaagcaacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    27001 agaggtgatt taaattcatc cccggcggca agccggggag atttcattac ggcagtggcg
    27061 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    27121 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    27181 gttttacggt aacgttagtg agatcatcag acatcaatgc caacttaccc ggcacagcaa
    27241 tcacccgctg ccactgacgg caatccagag tatcaccctc tttagtgaca atcaggctgc
    27301 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag tagcccgcca
    27361 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    27421 ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    27481 aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt cagagagaaa
    27541 tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc atgatgtatg
    27601 attcgcgact cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    27661 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    27721 tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca cgggtgaatt
    27781 tacccgttgg ggcggccagc tcctgcagct gtttggcgtc catgctgaag agtggggtta
    27841 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggga tgatgatcct
    27901 cggcatgttt ggcggctgct ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat
    27961 gccacgcagc cgtatccgca ttatgcaggc cattattggc ggtattatcg ctggttttgg
    28021 cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggtattc ctcagttctc
    28081 gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcacgctt
    28141 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    28201 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    28261 cggtatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat
    28321 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc
    28381 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    28441 ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat
    28501 gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc
    28561 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    28621 ggtcggtctg ggcaatgtga tcggctcgac gattctggcg tattactggg atgatttcgc
    28681 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg gcccgatggg
    28741 tggcctgctg gtgacatatt tgctgttgtt tactgcgctg atgttgatta tcggctggga
    28801 aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat gaaaaatatc
    28861 gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc agtcgcaacc
    28921 cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg aagtggtgag cgactgtcca
    28981 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt gcgggatatt
    29041 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    29101 gcccacagcg ggaacatcgt tgcctctgac acatagattt tacccgcccc ctctcctatt
    29161 cttttcagta tgatctcagg aggtttctat gtgtggacgc tttgcccaat cccaaacgcg
    29221 tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct acgatcccga
    29281 acccattggc agatacaacg tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga
    29341 tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt gggataaacc
    29401 gccgctgatt aacgcccgcg tagaaactgc cgcaaccagt cgcatgttta aaccgctctg
    29461 gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa aagaaggcga
    29521 caaaaaacag ccttttttta tctatcgtgc tgatggacaa cctattttta tggccgcgat
    29581 aggtagcaca ccatttgagc atggtgacga agccgaagga tttttaattg tcactgctgc
    29641 agcagaccag ggtctggtag acattcatga ccgccgcccg ctggttctgt cgccagaagc
    29701 tgcacaggaa tggatgcggc aagagattgg cggtaaagaa gcatcagaaa ttgctaccaa
    29761 cggctgtgtg ccagcaaacc agttcacctg gcacccggta tcgcgcgcgg taggtaatgt
    29821 caaaaaccag ggcgcggagt taattcaacc tgtttgttga catcctccac gccctgaagg
    29881 acgaggctcc ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt tcctgcttca
    29941 acgggcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc gccgctaaaa
    30001 tgttacgagc
//
LOCUS       DAJVTY010000031        29243 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVTY010000031 DAJVTY010000000
VERSION     DAJVTY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29243
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.031"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..145
                     /locus_tag="OQQ66_001277"
     CDS             <1..145
                     /locus_tag="OQQ66_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCS1904270.1"
                     /translation="GGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKS
                     FDW"
     gene            420..3269
                     /gene="rcsC"
                     /locus_tag="OQQ66_001278"
     CDS             420..3269
                     /gene="rcsC"
                     /locus_tag="OQQ66_001278"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1904271.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3469..4119)
                     /gene="rcsB"
                     /locus_tag="OQQ66_001279"
     CDS             complement(3469..4119)
                     /gene="rcsB"
                     /locus_tag="OQQ66_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1904272.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4136..6808)
                     /gene="rcsD"
                     /locus_tag="OQQ66_001280"
     CDS             complement(4136..6808)
                     /gene="rcsD"
                     /locus_tag="OQQ66_001280"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1904273.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7547..8677
                     /gene="ompC"
                     /locus_tag="OQQ66_001281"
     CDS             7547..8677
                     /gene="ompC"
                     /locus_tag="OQQ66_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1904274.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8789..9844
                     /gene="apbE"
                     /locus_tag="OQQ66_001282"
     CDS             8789..9844
                     /gene="apbE"
                     /locus_tag="OQQ66_001282"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1904275.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9918..10970
                     /gene="ada"
                     /locus_tag="OQQ66_001283"
     CDS             9918..10970
                     /gene="ada"
                     /locus_tag="OQQ66_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1904276.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10970..11620
                     /gene="alkB"
                     /locus_tag="OQQ66_001284"
     CDS             10970..11620
                     /gene="alkB"
                     /locus_tag="OQQ66_001284"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1904277.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11696..13339
                     /gene="yojI"
                     /locus_tag="OQQ66_001285"
     CDS             11696..13339
                     /gene="yojI"
                     /locus_tag="OQQ66_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1904278.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13557..15203
                     /gene="mqo"
                     /locus_tag="OQQ66_001286"
     CDS             13557..15203
                     /gene="mqo"
                     /locus_tag="OQQ66_001286"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS1904279.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15353..15841)
                     /gene="eco"
                     /locus_tag="OQQ66_001287"
     CDS             complement(15353..15841)
                     /gene="eco"
                     /locus_tag="OQQ66_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1904280.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15976..16140)
                     /locus_tag="OQQ66_001288"
     CDS             complement(15976..16140)
                     /locus_tag="OQQ66_001288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904281.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16249..16743
                     /gene="napF"
                     /locus_tag="OQQ66_001289"
     CDS             16249..16743
                     /gene="napF"
                     /locus_tag="OQQ66_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1904282.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16733..16996
                     /gene="napD"
                     /locus_tag="OQQ66_001290"
     CDS             16733..16996
                     /gene="napD"
                     /locus_tag="OQQ66_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1904283.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            16993..19479
                     /gene="napA"
                     /locus_tag="OQQ66_001291"
     CDS             16993..19479
                     /gene="napA"
                     /locus_tag="OQQ66_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1904284.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19486..20181
                     /gene="napG"
                     /locus_tag="OQQ66_001292"
     CDS             19486..20181
                     /gene="napG"
                     /locus_tag="OQQ66_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1904285.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20168..21031
                     /gene="napH"
                     /locus_tag="OQQ66_001293"
     CDS             20168..21031
                     /gene="napH"
                     /locus_tag="OQQ66_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1904286.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVVGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21028..21477
                     /gene="napB"
                     /locus_tag="OQQ66_001294"
     CDS             21028..21477
                     /gene="napB"
                     /locus_tag="OQQ66_001294"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1904287.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21487..22089
                     /gene="napC"
                     /locus_tag="OQQ66_001295"
     CDS             21487..22089
                     /gene="napC"
                     /locus_tag="OQQ66_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1904288.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22108..22725
                     /gene="ccmA"
                     /locus_tag="OQQ66_001296"
     CDS             22108..22725
                     /gene="ccmA"
                     /locus_tag="OQQ66_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1904289.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22722..23384
                     /gene="ccmB"
                     /locus_tag="OQQ66_001297"
     CDS             22722..23384
                     /gene="ccmB"
                     /locus_tag="OQQ66_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1904290.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23426..24163
                     /gene="ccmC"
                     /locus_tag="OQQ66_001298"
     CDS             23426..24163
                     /gene="ccmC"
                     /locus_tag="OQQ66_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1904291.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24160..24369
                     /gene="ccmD"
                     /locus_tag="OQQ66_001299"
     CDS             24160..24369
                     /gene="ccmD"
                     /locus_tag="OQQ66_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1904292.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24366..24845
                     /gene="ccmE"
                     /locus_tag="OQQ66_001300"
     CDS             24366..24845
                     /gene="ccmE"
                     /locus_tag="OQQ66_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1904293.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24842..26785
                     /gene="ccmF"
                     /locus_tag="OQQ66_001301"
     CDS             24842..26785
                     /gene="ccmF"
                     /locus_tag="OQQ66_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1904294.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            26782..27339
                     /gene="dsbE"
                     /locus_tag="OQQ66_001302"
     CDS             26782..27339
                     /gene="dsbE"
                     /locus_tag="OQQ66_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1904295.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27336..28388
                     /gene="ccmH"
                     /locus_tag="OQQ66_001303"
     CDS             27336..28388
                     /gene="ccmH"
                     /locus_tag="OQQ66_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1904296.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28423..29070)
                     /gene="narP"
                     /locus_tag="OQQ66_001304"
     CDS             complement(28423..29070)
                     /gene="narP"
                     /locus_tag="OQQ66_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1904297.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6676 a         7217 c         8104 g         7246 t
ORIGIN      
        1 tggtgggcgc agcgaaacgg aattaaccac cgcccaactg acgctgatca ccgatgaagg
       61 atcggtcaat gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac
      121 gctggtgaaa agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt
      181 cagataagac tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca
      241 gattggtcat ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt
      301 aaggcaatgg tttctaagcg atacgcatta ttgcaataat gacagaacat tgcggtaaca
      361 cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc ttcgcccctt
      421 tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat
      481 tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt
      541 tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc
      601 gctttattca acgcacctct gatgtgatga aagagctgaa gtacatcgcc gaaaatcgct
      661 tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg
      721 cgtttgaacc gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt
      781 ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg
      841 atctaaaccg ggtattttta atcggcagcg ataacctctg catggccaat ttcggtctgc
      901 gtgatatgcc agtggaacgc gataccgcgt tgaaagcttt gcatgaacgc atcaataaat
      961 atcgaaatgc accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc
     1021 gccctggcgt cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg
     1081 cgcttttggg ggtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc
     1141 cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag
     1201 aaagtaaaat taagggcgag cctcgctgga tgcaggaacg ctcctggttt ggctatacgg
     1261 aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt
     1321 attcggtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt
     1381 tgctgaatgt gcttgccgga gctgcattgt ttactctcgc acggatgtac gagcgacgta
     1441 ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca
     1501 agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata
     1561 ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac
     1621 gactgacgca aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca
     1681 ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt
     1741 gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac
     1801 aagcagcgga acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc
     1861 tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac
     1921 cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa
     1981 ttatcagcga tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc
     2041 gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg
     2101 tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg
     2161 gcgacccgat gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca
     2221 ccgataccgg ctgtatagtt ttgcatgtcc gcgctgatgg cgattatctc tctatccgtg
     2281 ttcgcgatac cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct
     2341 tccaggtcgg aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt
     2401 gtgaaaaact gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg
     2461 gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg
     2521 tggaagggtt gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt
     2581 tcctggaaac tagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac
     2641 cgactcccga agatgtgtta attactgacg aggtagtgag taaaaaatgg cagggtagag
     2701 cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt
     2761 gggtacacag tgtggctgcc ccgcatgagc taccggcatt gttggcgcgt atttatttga
     2821 tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg
     2881 ataatgacga tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg
     2941 cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggt gtcgatgcgc
     3001 ttaatgtact tagcaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata
     3061 tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg
     3121 gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca
     3181 gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg
     3241 agagggtcag gaaatcgcgg gaatcgtagg acggataagg tgtttatgtc gcatctggca
     3301 ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt tgatgtaatc
     3361 tgttgaaata atgggaatcg taggacgcat aaggcgttta cgccgcatcc ggcaatcggt
     3421 gcaaatgcca gataagacac taacgcgtct tatctggcct acaggtgatt agtctttatc
     3481 tgccggactt aacgtcactg aagagagata attcagcagg gcgatatcgt tctcgacacc
     3541 cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt
     3601 tttagcgatc tcggtcacca agaagccttc cgcaaacagg cgcagaactt cactctcttt
     3661 tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac
     3721 gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt
     3781 cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat
     3841 cgccgggttg ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat
     3901 gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat
     3961 caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc
     4021 gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat
     4081 cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca
     4141 gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct
     4201 tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca
     4261 tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg
     4321 ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat
     4381 agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt
     4441 cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat
     4501 tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca tcatcgctta
     4561 aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata tcataatctt
     4621 gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct aactggcgag
     4681 tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta
     4741 aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt
     4801 agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgcgcca
     4861 gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg ttttggggct
     4921 ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt
     4981 ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga
     5041 tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta
     5101 aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt
     5161 gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct
     5221 gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg ttcgctaact
     5281 gtatttcatc gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg
     5341 gggcgttgag tttagccgcg ctctccgcca gggactgtgc gggttctttc agcgcatcgc
     5401 caatgttttt cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct
     5461 gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg
     5521 tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg
     5581 cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcaaatgcg
     5641 gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa
     5701 ccagcaggcc gagcggcagc agtgagacta tctcttcatt aattgcccgt aaaatgcgca
     5761 attcgttatt gaccgccgtg ctgggtagac tttctgtact gcggctggag aaatggcgga
     5821 atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca
     5881 gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca
     5941 tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga
     6001 cgctatccgt cccttctttc tcattatcat tgtctcccgt tgccgtcgcg tctggctcaa
     6061 ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggt agatcaaaag
     6121 ctacgaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac
     6181 cgttctgcca ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg
     6241 cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgatggtc gattctttaa
     6301 atccggaggt gagatctttg aggggcaggg ttgagatcag caccagactg ttatcctgac
     6361 cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgtg tccagatagg
     6421 tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt
     6481 ccgttttgcg gcgcggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta
     6541 aaccttcgcc ggaggagggg gaggttgtcg cggcaatgtt gtcgtagatc tgccaggtca
     6601 cgtaacgcca ggtattgacg cgcttttgga tagcgtgggt aatgtcgaca atctggtaac
     6661 ttttatcttt cagccatgcg ttaacggcgc tttgtaccat tacacccatc gtcaccagta
     6721 acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg
     6781 tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag
     6841 tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc
     6901 tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac aagcgccatt
     6961 ttgctgtaca aataacctac aaaaagccca acgaaattaa ttgcaaataa acgatgattg
     7021 ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct
     7081 gtggtagcac agaataatga aaagtgtgta aagaagggta aaaaaaaccg gatgcgaggc
     7141 atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat aaataaagtt
     7201 aatgatgata gcgggagtta ttctagttgc gagtgaaggt tttgttttga cattcagtgc
     7261 tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg cttgatgtta
     7321 ggtgcttatt tcgccattcc acaataatct taaaaagttc ccttgtattt acattttgaa
     7381 acatctatag cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta
     7441 ttctgcattt ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca
     7501 gtggcataaa aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt
     7561 actgtccctc ctggtcccag ctctgctggt agcaggcgca gcaaacgctg ctgaagttta
     7621 caacaaagac ggcaacaaat tagatctgta cggtaaagta gacggcttgc actatttctc
     7681 tgacaacaag tcagaagacg gcgaccagac ctatgtacgt cttggtttca aaggcgaaac
     7741 tcaggttact gaccagctga ccggttacgg ccagtgggaa tatcagatcc agggcaatac
     7801 ctctgaagac aacaaagaaa actcctggac ccgtgtggca ttcgcaggtc tgaaattcca
     7861 ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg taacttcctg
     7921 gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact tcatgcagca
     7981 gcgtggtaac ggcttcgcga cctaccgtaa caccgacttc ttcggtctgg ttgacggtct
     8041 gaactttgct gttcagtacc agggcaaaaa cggtagcgta agcggcgaag gcatgaccaa
     8101 caacggtcgt ggcgctctgc gtcagaacgg cgacggtgtc ggcggttcta tcacttatga
     8161 ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg atgatcaaaa
     8221 tggtagctac atcagcaatg gtgtagttcg taactacatc ggtactggcg accgtgctga
     8281 aacctacact ggtggtctga aatacgacgc taacaacatc tacctggctg ctcagtacac
     8341 ccagacctac aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag cacagaactt
     8401 cgaagctgtt gctcagtacc agttcgactt cggtctgcgt ccgtctgtag catacctgca
     8461 gtctaaaggt aaaaacctgg gtgtcatcaa tagtcgtaac tacgacgacg aagatatcct
     8521 gaaatatgtt gatgttggcg cgacctacta cttcaacaaa aacatgtcca cctatgttga
     8581 ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca tcaacactga
     8641 taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat cgaacaaagg
     8701 gcctgcgggc ccttttttca ttgttttcag cgtacaaact cagttttttg gtgtactctt
     8761 gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg tggcactgct
     8821 ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caacccgcca aaacccacgc
     8881 tactgaagtt accgttcttg aaggcaaaac catgggtacc ttctggcgtg ccagcatccc
     8941 gggcattgat gccaaacgca gtgccgaact taaagaaaag attcagaccc agctggacgc
     9001 cgacgatcag ctgctttcga cctataaaaa agattccgcg ctgatgcgct ttaacgactc
     9061 gcaaagtttg tcgccgtggc cagtaagtga agcgatggcc gaaatcgtca ctacctcgct
     9121 gcgcattggc gcgaagaccg atggtgcgat ggatattacc gtcgggccgc tggtaaatct
     9181 gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac agatcgacga
     9241 gatgaaagcc aaaactggtt tacagcacct tacggtcatt aatcagtcgc atcagcaata
     9301 tctgcaaaaa gacctgccgg atttatatgt cgatctctct accgtcggcg aaggttatgc
     9361 ggcggatcac ttggcacgct tgatggagca ggaagggatt tcccgctatc tggtgtcggt
     9421 gggcggcgcg ctgaacagcc gtggtatcaa cggtgaaggc ctgccgtggc gggtagcgat
     9481 tcaaaaacca accgataaag aaaacgcagt tcaggctgtg gtggatatca acggtcatgg
     9541 gattagcacc tctggcagct accgtaacta ttacgaactg gacggcaaac gtctttccca
     9601 tgttatcgat ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg tgacggtgat
     9661 tgctccgacg gcgctggaag ccgatgcctg ggatactggc ttgatggtac tcgggccgga
     9721 gaaagccaaa gaggttgttc gccgggaagg gctggcggtt tatatgatca ccaaagaagg
     9781 cgatagcttt aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa
     9841 ttaaagcgca agattgttgg tttttgcgtg atggtgaccg ggcagactaa aggctatcct
     9901 taaccaggga gctgattatg aaaaacgcca catgcttaac tgacgatcaa cgctggcaat
     9961 ctgtcttagc ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg cgcaccacag
    10021 gcatcttttg ccgtccgtct tgccgcgcca gacatgcctt gcgggaaaat gtctccttct
    10081 acgcaaatgc cagcgaggca ctcgccgccg gatttcgccc ctgcaaacgc tgccaacctg
    10141 ataaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt cgactgctgg
    10201 aacaggaaac gcctgtaacg ttggaagcct tagccgacca ggtggcgatg agtccatttc
    10261 atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg caacaggcct
    10321 ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg acgtctattc
    10381 ttaacgccgg attccccgac agcagcagtt actaccgcaa agccgatgaa acgctgggca
    10441 tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac gcgctggctg
    10501 attgtgcgct gggtcgttgc ctggtggcag aaagcgagcg ggggatttgc gcgatattgc
    10561 tgggcgatga tgacgcgaca ttaatcagcg agttgcagca gatgtttccc gctgccgaca
    10621 acgcgcctgc cgatctgacg tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc
    10681 aacgcgatac gccgctgacg ttaccgctgg atattcgcgg cactgctttt cagcaacaag
    10741 tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt actggctaac gccatcggca
    10801 aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc caacaagctg gctatcgtaa
    10861 taccctgtca tcgagtggtc ggtggtgatg gcacactttc cggttaccgc tggggcgtgt
    10921 ctcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa tgttggatct
    10981 gtttgccgat gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa ttttacggcg
    11041 ttttgctttt aacgctgcgg agcaactgat ccgcgatatt aatgacgttg ccagccagtc
    11101 gccgtttcgc cagatggtca cccccggggg atataccatg tcggtggcga tgaccaactg
    11161 tgggcatctg ggctggacga tccatcggca aggttatctc tattcgccca ttgatccgca
    11221 aacaaataaa ccgtggcccg ccatgccaca aagctttcat gatttatgtc aacgtgcggc
    11281 tacggcggcg ggctatccag atttccagcc agatgcttgt cttatcaacc gctacgctcc
    11341 tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg cgccaattgt
    11401 ttctgtttct ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac gtaatgatcc
    11461 gctcaaacgt ttgttgttgg aacatggcga tgtggtggta tggggcggtg aatcgcggct
    11521 gttttatcac ggtattcaac cgttgaaagc ggggttttat ccactcacca ccgactgccg
    11581 ctacaacctg acattccgtc aggcaggtaa aaaagaataa aaataagaat tattattgct
    11641 gtgcgcgcga agatgtttaa actgcgggct gtaattcatt gtccgggttt tctgcatgga
    11701 acttcttgta cttgtctggc ggcagtatcg ctggccattt atcagtgtga tggcgctaag
    11761 cctcgccagt gcggcattag gcattggctt aattgctttt atcaatcagc gccttatcga
    11821 aatggcggat accagtctgc tggtattgcc ggagtttctg ggattattgc tgctgttgat
    11881 ggcagtcact ctcggatcgc aactggcgct caccactttg gggcatcact tcgtttaccg
    11941 actgcgtagt gaatttatca agcggattct ggatacccac gtcgagcgca ttgaacaact
    12001 cggtagcgcc tcgttgttgg cgggattaac cagcgatgtg cgcaatatca ccattgcttt
    12061 tgtgcgtctt ccggaactgg tgcaggggat cattctcact atcggttccg cggcgtatct
    12121 gtggatgctg tcgggcaaaa tgttgctggt aacggctatc tggatggcga tcaccatctg
    12181 gggtggtttt gtactggtgg cgcgggtgta caaacatatg gcgaccctac gtgaaaccga
    12241 agacaagctg tacacggatt ttcaaactgt acttgaaggg cgcaaagagc tgactctgaa
    12301 ccgggaacgc gccgagtatg tgtttaacaa cctctacatc cccgatgcgc aagagtatcg
    12361 ccatcatatt attcgcgcag acaccttcca tcttagtgcc gtgaactggt caaacatcat
    12421 gatgctgggc gctatcggcc tggtgttctg gatggcgaac agtctcggtt gggctgatac
    12481 caacgttgct gcgacctatt cgttgacgct tttattcctg cgtacgccgc tgctttcggc
    12541 ggttggcgca ttgccgacgc tgctgatggc gcaggtggcg tttaacaagc tgaacaaatt
    12601 cgcgctcgcg cctttcaaag cagagtttcc gcgcccgcag gcgtttccca actggcaaac
    12661 gctggagctg cgtaatgtga cgtttagcta tcaggataac gcgttttccg ttggtccgat
    12721 taatctcacc atcaaacgtg gcgagctact gtttctgatt ggtggtaacg gtagtggaaa
    12781 atcgacgctg gcgatgttgt tgacgggctt gtatcagcca caaagcggcg aaatcttgct
    12841 ggatggcaaa cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt tttcggcagt
    12901 gtttaccgat gtctggcttt ttgatcaact gctggggccg gagggtaaac ccgctaaccc
    12961 gcaactggtt gagaagtggc tggcgcagct gaaaatggct cataagcttg agttaagcaa
    13021 cgggcgtatt gttaacctga agttatcaaa agggcagaaa aaacgcgtgg cgctgttgct
    13081 ggcgctggca gaagaacgcg atattatcct gctggatgaa tgggcggcgg atcaggatcc
    13141 acacttccgt cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga tgggtaaaac
    13201 tattttcgct atcagtcatg atgatcatta ctttatccac gccgaccgcc tgctggaaat
    13261 gcgcaatggg caacttagcg agctgacggg cgaagagcgc gatgccgctt cgcgtgatgc
    13321 cgttgcccgg acggcataat attacgacgc tgacctggcg gactgctgcc gtcaggtcaa
    13381 tatgctaaaa atcccttcat ttctaataac cctataatta atcaacgaaa ttataatgtt
    13441 tctaaaatta gaatataatt tataaacatt atttaaatgt tgttacttaa gtgttacctt
    13501 tgatgccgcg caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga
    13561 aaaaagtgac tgccatgctc ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg
    13621 caaaagcgaa agcgtccgag gagcaggaaa ctgatgtact gttgattggc ggcggcatta
    13681 tgagcgccac gttggggacc tatttacgcg agctggagcc tgaatggtcg atgaccatgc
    13741 tggagcgcct ggagggtgtc gcgcaggaga gttcgaacgg ctggaataac gccggaaccg
    13801 ggcattctgc actgatggaa ctgaactaca ccccgcaaaa cgccgatggc agtatcagta
    13861 ttgaaaaagc agtcgccatt aacgaagcat ttcagatttc ccgccagttc tgggcgcacc
    13921 aggtcgagcg cggcgtgctg cgtactccgc gttcatttat caataccgtt ccgcatatga
    13981 gctttgtctg gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac
    14041 aaagctcgct gtttcgcggt atgcgttact ctgaagatca cgcgcagatc aaagagtggg
    14101 caccgttagt gatggaaggg cgcgatccgc aacagaaagt ggcagccacg cgtacggaaa
    14161 ttggtaccga tgtgaactac ggcgagatca cccgccagtt aattgcttcc ttgcagaaga
    14221 aatctaactt ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt aatgacgata
    14281 acacctggac cgttaccgtt gccgatctga aaaatggcac tgcacagaac attcgtgcca
    14341 aatttgtctt tatcggcgcg ggcggtgcgg cgctgaagct gttacaggaa tcggggattc
    14401 cggaagcgaa agactacgcc ggtttcccgg tgggcggaca gttccttgtt tcggaaaacc
    14461 cggacgtggt taatcaccat ctggcgaagg tttacggtaa agcatccgtt ggcgcaccac
    14521 cgatgtcggt tccgcatatc gatacccgcg ttctggacgg taaacgcgta gtgctgtttg
    14581 ggccatttgc caccttctca accaaattcc tcaaaaacgg ttcattgtgg gatctgatga
    14641 gttccaccac cacctctaac gtgatgccga tgatgcacgt cgggctggat aatttcgatc
    14701 tggtgaaata tctggtgagt caggtgatgt tgagtgaaga ggatcgtttt gaagcgttga
    14761 aagagtacta tccgcaggcg aaaaaagagg actggcgttt gtggcaagcg gggcagcgtg
    14821 tgcagattat caagcgtgat gccgataaag gtggtgtact gcgtctgggt actgaagtgg
    14881 tcagtgacca gcaagggacc attgccgcgc tcctgggagc atcgccaggg gcgtcaaccg
    14941 ccgcgccgat tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt tccagcccgc
    15001 aatggcaggc tacgttgaaa gcgatcgttc cgtcttatgg acgcaagctg aacggtgatg
    15061 tagcggcaac agaacgcgag ttgcagtaca ccagcgaagt gctggggctg aaatacgaca
    15121 agccgcaggc agcagatagt acgccaaaac cgcagttgaa accgcaaccc gttcaaaaag
    15181 aagtggcgga tattgcgttg taatgatgtg ccacatccgg catggtatgc cggatgtggc
    15241 gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt tagatgttgg
    15301 atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca gtttagcgaa
    15361 cttctgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca tctacattgt
    15421 ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca gcatcgccca
    15481 gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc atcgtggaaa
    15541 ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc agcgttttgc
    15601 tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta
    15661 acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc acctgacgct
    15721 tcatcccttt ttcagcttgt ggataaggcg cgattttttc cagtggctgg acgctttctg
    15781 ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac tgcaggtaga atggtcttca
    15841 tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg aaggttaaca
    15901 tattcataag atttattaat ttgctttatg aataacttaa agaatattca gacaatttta
    15961 cctgctcttt tagtgttaaa ttctgatgag agaacaaacc agcagaaata tctctggtga
    16021 attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag cccgtttttg
    16081 ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg gggtatgcat
    16141 ctttgacaca tcctttaata tcttagcggt tataaaaatg gcttattaat tatgcggttt
    16201 atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt gaagattgat
    16261 gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg tatccgtccg
    16321 ccctggtccg gtgatgaatc tcattttctg acccattgca cccgttgcga cgcctgtatt
    16381 aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag cgttaatttc
    16441 aaaaataacg agtgcagctt ctgttacgct tgcgcgcagg cttgccccga atcgctattt
    16501 tctccgcgcc acaccagggc ctgggatttg cagttcacca ttggggacgc gtgcctggcg
    16561 tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc aattatcttt
    16621 cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg caacggatgt
    16681 ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct ccatgcacac
    16741 taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt cagacatcag
    16801 cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc cgagcggtca
    16861 gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca ttgagtcagt
    16921 acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg aagagcaagg
    16981 tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc cgttgcggcc
    17041 gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag
    17101 gaagccatca aatgggataa agcgccgtgc cgtttctgcg gtactggttg cggcgttctg
    17161 gtcggaacgc agcagggacg tgtggtggcc tgtcagggcg acccagacgc accggttaac
    17221 cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg taaagaccgt
    17281 ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg cgaatttacc
    17341 ctaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac cgccctgaaa
    17401 gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat ctgggaaggt
    17461 tatgccgcgt ccaagctgtt taaagcgggc ttccgttcga acaacatcga cccgaacgcg
    17521 cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat ggatgagccg
    17581 atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg cgcaaacatg
    17641 gcggagatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctctc taatcagaac
    17701 gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc ggataacggc
    17761 atcatcttta cgccacagtc tgacctggtg atcctgaact acatcgccaa ctatatcatt
    17821 caaaacaatg cgataaatca ggacttcttc agcaaacacg ttaacctgcg caaaggggcg
    17881 acggacattg gctacggttt acgtccgacc catccgctgg aaaaagcagc gaagaatcca
    17941 ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt tgccgagtat
    18001 acgctggaaa aaactgccga aatgaccggc gtgccgaaag accagttaga acaactggcg
    18061 cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg cttcaaccag
    18121 catactcgtg gcgtgtgggc taacaacctg gtctacaacc tgcacctgct gaccggcaaa
    18181 atttcccagc cgggttgcgg tccgttctcc ctgaccgggc aaccttctgc gtgtggtact
    18241 gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt gaccaacgag
    18301 aaacaccgtg atatctgcga gaagaagtgg aatatcccga gcggcaccat tccggcgaaa
    18361 atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaaact gaatgtttac
    18421 tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga gcgtatgccg
    18481 ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac agtcagtgcg
    18541 ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg cgcttacggt
    18601 aacgccgaac gccgtactca gttctggcgt caacaggtac aggcaccggg cgaagcgaaa
    18661 tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga agtatggccg
    18721 gaagatctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga agtgctgtat
    18781 gccacaccgg aagtgagcaa attcccggta tccgaactgg cggaagatca gctgaacgat
    18841 gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta cgcatggttt
    18901 ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc gcgcggtctg
    18961 cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg taacgacccg
    19021 tacgtgaaag cgggcgaagg ctataagttc tacggcaaac cggatggcaa agcggtgatc
    19081 ttcgcactgc cattcgaacc ggcggcggaa gcaccggatg aagagtacga cctgtggctc
    19141 tctaccggac gcgttctgga gcactggcac accggcagta tgactcgccg tgtaccagaa
    19201 ctgcaccgcg ccttcccgga agcggtcctg tttattcacc cgctggatgc gaaagcgcgc
    19261 gatctgcgtc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt gatctcgatt
    19321 gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc gttcttcgac
    19381 gccgcacagc tggttaacaa actaacgcag gacgcgaccg atccgctctc gaaagagacg
    19441 gacttcaaga agtgcgcagt caaactggag aaggtgtaag ccattatgtc ccggtcagcg
    19501 aaacctcaaa atggtcgccg ccgctttctg cgcgatgttg ttcgcacagc aggcgggctg
    19561 gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc tggcgtgcgg
    19621 ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt gcgttgcggt
    19681 cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc ctctggtctg
    19741 tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg tgaggacatt
    19801 ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc gatcgacgac
    19861 gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt tcaggggctg
    19921 cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac cctggagctg
    19981 gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca cagagacgcc
    20041 tgtaccggtt gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc aatcaaggtg
    20101 ttaccgctgt cactggctaa aggggagtta ggtcaccatt accgcttcgg ctggctggag
    20161 gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga aaaaaggctg
    20221 gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg tgctggggat
    20281 gtttttgagc ggtccgtggt ttggtgtgtg gatcttacac ggtaactaca gcagtagcct
    20341 gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc ttgccagcgg
    20401 tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc tctacgccct
    20461 ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aatccgatta ccgacttagc
    20521 gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc gccacattcg
    20581 ctacgtgctg ttggtggtca ttctgctggg atcggcctta actggcacgc tcatctggga
    20641 atggatcaac cccgtatctc tgatggggcg cagcctggta gtgggcttcg gcagcggcgc
    20701 gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg gctggtgcgg
    20761 gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg tgattaccgt
    20821 tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg tttgcccgga
    20881 accgcgtgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg tcaccagccg
    20941 cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat ttacaataac
    21001 tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa agcgctgtgt
    21061 caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc taatggcgtc
    21121 gattttagcc agtcgccgga agtatccggg acacaggaag gggccattcg gatgccaaaa
    21181 gagcaggaac ggatgccgct gaactatgtg aatcagccgc caatgatccc gcatagcgtt
    21241 gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg tgtcgaaagt
    21301 tatcgcacca ctggcgcgcc gcgcattagt cctactcact ttatggacag cgacggcaaa
    21361 gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt accgcaggcc
    21421 gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg gaaataagag
    21481 gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg gaaatggtgg
    21541 cgtaccccca gccgtctggc gctggggacg ctgctgttga tcggttttgt tggcggcatc
    21601 gtcttctggg gtggctttaa caccgggatg gaaaaagcca ataccgaaga gttctgcatt
    21661 agctgccacg aaatgcgcaa cacggtatat caggaataca tggattccgt gcactacaac
    21721 aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga gtttgtgccg
    21781 aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt tggcgttatt
    21841 gacacgccgc agaaatttga agctcatcgt ctgacgatgg cacagaatga gtggcggcgc
    21901 atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta tatggataca
    21961 accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga tgggcaaacc
    22021 tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgtga agtcgagcca
    22081 ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact ttgtgagcgg
    22141 gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga gtgggtacaa
    22201 atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac ggggttgtct
    22261 cgccctgacg caggcgaggt tctctggcaa gggcagccct tgcatcaggt acgcgacagc
    22321 taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg gctgacggcg
    22381 ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct ggaagccctg
    22441 gcgcaggccg ggcttgccgg attcgaagat attcctgtaa atcagctctc ggccgggcaa
    22501 caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg gatcctcgac
    22561 gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg tatggcgcag
    22621 catacggagc agggggggat tgtgattctg actacccacc agccgctcaa cgttgctgaa
    22681 agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc tggcgcattt
    22741 tccgtcttga gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac ccgctgtggt
    22801 tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg caactgctgg
    22861 cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg ctggcgctgg
    22921 aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg ttgttgccgt
    22981 tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta accggtctgc
    23041 cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt tatggctggc
    23101 aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg
    23161 gtgtggcgtt gacagtggga cttaagcgcg gtggtgtgct gctcagcata ctggtgttac
    23221 cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct tccatgcatt
    23281 tgcccgttga cgggtatctg gcaattttag gcgcgttgct ggcaggcacc gcgacattaa
    23341 gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg attactgatt
    23401 tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg cgatcccacc
    23461 acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca gtgtggtcgt
    23521 gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc agcagggaaa
    23581 tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg gtatttatgc
    23641 atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg ccaacctggc
    23701 ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg ttaccggctc
    23761 tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc tgacttctga
    23821 actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct tcgacgaccg
    23881 ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga atctgccgat
    23941 tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc ggatgcagca
    24001 aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct tcctgctcct
    24061 gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg
    24121 tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccttgc atttgcttcc
    24181 tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc ggtggtgatg
    24241 accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg cgcaattctg
    24301 tgtggtgtgg agcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag
    24361 gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtg ttggcaggtt
    24421 tggcgctgac catcggtctg gtgctgtatg cgctgcgctc gaatatcgat ctcttttata
    24481 cgccggggga aattctctac ggcaagcgtg aaactcagca aatgccggaa gtcggtcagc
    24541 gtctgcgcgt tggcgggatg gtgatgccgg gtagtgtgca gcgcgatccc aattcgctga
    24601 aagtgacctt caccatttac gacgctgaag gctcagtgga tgtctcttac gaaggcattt
    24661 tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg gaaaaaggca
    24721 atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg ccgccagaag
    24781 ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag gacccagcat
    24841 catgatgccg gaaattggaa acgggctgct gtgcctggcg ctgggaattg cgctgctgct
    24901 gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg cgtcttcccg
    24961 cttgtttgcc tggctgctgt ttatgtctgt ggctggcgca tttctggtgc tggtcaatgc
    25021 tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata cccagcttcc
    25081 ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggttcgcttc tgctgtgggt
    25141 gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta ttccgctgga
    25201 tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt tgctgttcat
    25261 tctctttacc tctaacccgt tctcacgcac gctgccgaac ttcccaattg aagggcgcga
    25321 tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc tttatatggg
    25381 gtacgtgggt ttctcggtgg cgtttgcttt tgccattgct tctttgctga gcgggcgtct
    25441 ggacagcact tatgcgcgtt ttactcgccc gtggacgctg gcggcgtgga ttttcctgac
    25501 gctcggcatt gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg ggggctggtg
    25561 gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga ctgcgctgat
    25621 gcactcactg gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat tactgctggc
    25681 aatcagtgcc ttctcgttgt gtctgctggg gactttcctg gtgcgttccg gcgtgctggt
    25741 atcggtacac gcgtttgcct ctgatccggc acgcggtatg tttatcctcg cctttatggt
    25801 actggtgatt ggtggttcgc tgctgctgtt tgccgcgcgt ggacacaaag ttcgctcacg
    25861 cgtaaacaat gcgctgtggt cgcgggaatc tctgctgtta gcgaacaacg ttttgttggt
    25921 cgccgcgatg ctggtggtat tactggggac gctgctgccg ctggtgcaca agcaactggg
    25981 actgggcagt atttcgattg gcgaaccgtt cttcaacacc atgtttacct ggctgatggt
    26041 gccgtttgcg ctgctgcttg gtgtcggtcc tctggtgcgc tgggggcgcg atcgcccacg
    26101 taaaatccgc aatttattga ttatcgcctt catctcaacg ctggtgctgt cgctgctgct
    26161 gccgtggctg ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg caatggcctg
    26221 ctggattgct gtgctggcaa ttgcggaagc tgcgctgcgt atttcacgtg gcacgaaaac
    26281 caccttcagt tattggggaa tggtggcggc tcacctgggg ctggcagtga caattgttgg
    26341 tattgccttt agccagaact atagcgttga gcgtgatgtg cgcatgaagt ccggcgatag
    26401 cgtcgatatt catgaatatc gcttcacctt ccgtgatgtc aaagaggtga ctggcccgaa
    26461 ctggcgcggc ggtgtggcga ctattggcgt aacgcgagag ggtaaaccgg aaacggtgct
    26521 gtatgcggaa aaacgttatt acaacactgc cgggtcgatg atgaccgaag cggcgattga
    26581 cggcggcatc acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa acggcgcgtg
    26641 ggccgtgcgt ctttactaca aaccgtttgt tcgctggatt tgggcgggcg ggctgatgat
    26701 ggcgttgggc ggactgctgt gtctgtttga tcctcgctat cgtaagcgcg tgagtccgca
    26761 aaaaactgcg ccggaggctg tatgaagcgc aaagtattgt taattccgtt gattatcttc
    26821 ctggcgattg ccgcggcgct gctgtggcag ctggcgcgta atgccgaagg ggatgatccg
    26881 accaatctgg aatcggcgct cattggcaag ccagtgccga agtttcgcct cgaatcactg
    26941 gacaatccgg ggcagtttta tcaggcggat gtgctgactc agggcaaacc tgtgctgctt
    27001 aacgtttggg ctacctggtg tccgacctgc cgtgcggaac atcaatatct gaatcagctt
    27061 tctgcgcagg gcattcgcgt ggtcggcatg aactataaag acgatcgcca gaaggcaata
    27121 agctggctga aagagctggg caatccttac gcgctaagcc tgtttgatgg cgatggcatg
    27181 ttagggctgg atctcggtgt ctatggcgcg ccagaaacgt tcctcatcga cggcaacggc
    27241 atcattcgct atcgccatgc gggcgatctc aatcctcgcg tctgggaaga agagatcaag
    27301 ccgctgtggg agaaatacag taaggaggcc gcacaatgag gtttttattg ggcgtgctga
    27361 tgctgatgat ctccggctca gcgctggcga ccatcgacgt gttgcagttt aaggatgaag
    27421 cgcaggagca gcagttccgc caactcactg aagaactacg ctgcccgaaa tgccagaaca
    27481 acagcattgc cgattccaac tcgatgattg ccaccgacct gcgccagaaa gtgtatgaac
    27541 tgatgcagga aggtaaaagt aaaaaagaga ttgtcgatta tatggtggcg cgttacggca
    27601 acttcgtcac ttacgatccg ccgttaacgc cgctgaccgt gctgctgtgg gtgcttccgg
    27661 tagtggctat tggcattggc ggttgggtca tttacgcccg ttcgcggcgt cgggtacgcg
    27721 tggtgccgga cgcgtttcct gaacaaagcg tgccggaagg taagcgtgcc ggatatattg
    27781 tttatctgcc gggtattgtg gtggcgttaa ttgtggctgg cgtcagctac taccagactg
    27841 gcaattatca gcaggtgaaa atctggcagc aggccacggc acaggctccg gcgttgctgg
    27901 acagggcgct ggatccgaaa gccgatccgc tcaacgaaga agagatgtcg cgtcttgcgc
    27961 tggggatgcg tactcaactg caaaaaaatc cgggagatat agaaggctgg attatgttgg
    28021 gccgcgttgg catggcgctg ggtaacgcca gtatcgccac cgatgcatac gctactgcgt
    28081 atcgcctcga tccgaaaaac agtgatgccg cactgggtta tgctgaagcg ttgacacgtt
    28141 catctgatcc caacgacaac cgcctcggtg gtgaactgct gcgccagttg gtgagaacgg
    28201 accatagcaa tatccgtgtg ttaagcatgt atgcgtttaa tgcctttgag cagcagcgat
    28261 ttggcgaagc cgttgccgcg tgggagatga tgttgaaact cttatctgcc aacgatactc
    28321 gccgtgcggt gattgaacgt agtatcgcgc aggcgatgca acatttgtcg ccgcaggaga
    28381 gtaaataaaa agaggcccga tggtgatgcc atcgggctat ttttattgtg ccccgcgttg
    28441 ttgcaggaac agaatggtgg ctgccacgcg tgagcggaca ttgagtttgc gcagcagatt
    28501 gcgaatatgt acttttactg tctgctctga aatattcaac accgaggcaa tctgtttatt
    28561 tgacagcccc tgtgccagct cgtgcagaac atccagctcg cgctccgtca gcacgctgaa
    28621 gggatcttct tccgcgccaa acatttcacg ttcacgtaag tactgattga cgcgttcgct
    28681 aaagactttg ctgcctttcg ctccagcacg aattgcttcc agcaatactt ccgggtcgct
    28741 gtctttcaac agataaccgt ctgcgcctgc gtctatcagc gcaaagacat cgctggaggc
    28801 atctgatacg gtcaggataa taatttgcgc ggtaacgcca tccctgcgca aggcattgag
    28861 ggtatccagg ccactcatac ctttcatatt gagatccagc aagatcacgt cgatatccag
    28921 tctatttgcc agatcgatcg cgctcgcgcc gtcgcccgct tcggcgacca cttcaaagct
    28981 aggatcaagc tccagtaact gacgaacacc gcgtcgcata agtggatgat catccacaat
    29041 catcacctga aaaggcgttg cttcaggcat agtagtctcc aggggtttta ttagaatcat
    29101 tatgtttgag cgtagcgttg gtaacgctac caggcgcgtg aatgttaccc cattttatgt
    29161 atggacatgt agtgaaaact acaaccttgt cgttcgcttt ggcatcattt tcattataaa
    29221 gacggaattt tgcatcagct tct
//
LOCUS       DAJVTY010000032        28703 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVTY010000032 DAJVTY010000000
VERSION     DAJVTY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28703
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.032"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            152..595
                     /locus_tag="OQQ66_001305"
     CDS             152..595
                     /locus_tag="OQQ66_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1904298.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            831..1649
                     /gene="ybhA"
                     /locus_tag="OQQ66_001306"
     CDS             831..1649
                     /gene="ybhA"
                     /locus_tag="OQQ66_001306"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1904299.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1650..2708)
                     /gene="modC"
                     /locus_tag="OQQ66_001307"
     CDS             complement(1650..2708)
                     /gene="modC"
                     /locus_tag="OQQ66_001307"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1904300.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2711..3400)
                     /gene="modB"
                     /locus_tag="OQQ66_001308"
     CDS             complement(2711..3400)
                     /gene="modB"
                     /locus_tag="OQQ66_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1904301.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3400..4173)
                     /gene="modA"
                     /locus_tag="OQQ66_001309"
     CDS             complement(3400..4173)
                     /gene="modA"
                     /locus_tag="OQQ66_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1904302.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4339..4488)
                     /gene="acrZ"
                     /locus_tag="OQQ66_001310"
     CDS             complement(4339..4488)
                     /gene="acrZ"
                     /locus_tag="OQQ66_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1904303.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4617..5405
                     /gene="modE"
                     /locus_tag="OQQ66_001311"
     CDS             4617..5405
                     /gene="modE"
                     /locus_tag="OQQ66_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1904304.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5473..6945
                     /gene="modF"
                     /locus_tag="OQQ66_001312"
     CDS             5473..6945
                     /gene="modF"
                     /locus_tag="OQQ66_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1904305.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7206..8222
                     /gene="galE"
                     /locus_tag="OQQ66_001313"
     CDS             7206..8222
                     /gene="galE"
                     /locus_tag="OQQ66_001313"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1904306.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8232..9278
                     /gene="galT"
                     /locus_tag="OQQ66_001314"
     CDS             8232..9278
                     /gene="galT"
                     /locus_tag="OQQ66_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1904307.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9282..10430
                     /gene="galK"
                     /locus_tag="OQQ66_001315"
     CDS             9282..10430
                     /gene="galK"
                     /locus_tag="OQQ66_001315"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1904308.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLAEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10424..11464
                     /gene="galM"
                     /locus_tag="OQQ66_001316"
     CDS             10424..11464
                     /gene="galM"
                     /locus_tag="OQQ66_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1904309.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11666..12418
                     /gene="gpmA"
                     /locus_tag="OQQ66_001317"
     CDS             11666..12418
                     /gene="gpmA"
                     /locus_tag="OQQ66_001317"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1904310.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12576..13628)
                     /gene="aroG"
                     /locus_tag="OQQ66_001318"
     CDS             complement(12576..13628)
                     /gene="aroG"
                     /locus_tag="OQQ66_001318"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1904311.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13944..14324
                     /locus_tag="OQQ66_001319"
     CDS             13944..14324
                     /locus_tag="OQQ66_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1904312.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14438..15379
                     /gene="zitB"
                     /locus_tag="OQQ66_001320"
     CDS             14438..15379
                     /gene="zitB"
                     /locus_tag="OQQ66_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1904313.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15376..16095)
                     /gene="pnuC"
                     /locus_tag="OQQ66_001321"
     CDS             complement(15376..16095)
                     /gene="pnuC"
                     /locus_tag="OQQ66_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1904314.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16133..17176)
                     /gene="nadA"
                     /locus_tag="OQQ66_001322"
     CDS             complement(16133..17176)
                     /gene="nadA"
                     /locus_tag="OQQ66_001322"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1904315.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17425..17500)
                     /locus_tag="OQQ66_001323"
     tRNA            complement(17425..17500)
                     /locus_tag="OQQ66_001323"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Lys,seq:ttt)
     gene            complement(17647..17722)
                     /locus_tag="OQQ66_001324"
     tRNA            complement(17647..17722)
                     /locus_tag="OQQ66_001324"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17687..17689),aa:Lys,seq:ttt)
     gene            complement(17726..17801)
                     /locus_tag="OQQ66_001325"
     tRNA            complement(17726..17801)
                     /locus_tag="OQQ66_001325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17766..17768),aa:Val,seq:tac)
     gene            complement(17853..17928)
                     /locus_tag="OQQ66_001326"
     tRNA            complement(17853..17928)
                     /locus_tag="OQQ66_001326"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17893..17895),aa:Lys,seq:ttt)
     gene            complement(17931..18006)
                     /locus_tag="OQQ66_001327"
     tRNA            complement(17931..18006)
                     /locus_tag="OQQ66_001327"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17971..17973),aa:Val,seq:tac)
     gene            complement(18042..18117)
                     /locus_tag="OQQ66_001328"
     tRNA            complement(18042..18117)
                     /locus_tag="OQQ66_001328"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18082..18084),aa:Lys,seq:ttt)
     gene            complement(18282..19073)
                     /gene="cpoB"
                     /locus_tag="OQQ66_001329"
     CDS             complement(18282..19073)
                     /gene="cpoB"
                     /locus_tag="OQQ66_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1904316.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19083..19604)
                     /gene="pal"
                     /locus_tag="OQQ66_001330"
     CDS             complement(19083..19604)
                     /gene="pal"
                     /locus_tag="OQQ66_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1904317.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19639..20931)
                     /gene="tolB"
                     /locus_tag="OQQ66_001331"
     CDS             complement(19639..20931)
                     /gene="tolB"
                     /locus_tag="OQQ66_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1904318.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21064..22245)
                     /gene="tolA"
                     /locus_tag="OQQ66_001332"
     CDS             complement(21064..22245)
                     /gene="tolA"
                     /locus_tag="OQQ66_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1904319.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNSKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22310..22738)
                     /gene="tolR"
                     /locus_tag="OQQ66_001333"
     CDS             complement(22310..22738)
                     /gene="tolR"
                     /locus_tag="OQQ66_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1904320.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22742..23434)
                     /gene="tolQ"
                     /locus_tag="OQQ66_001334"
     CDS             complement(22742..23434)
                     /gene="tolQ"
                     /locus_tag="OQQ66_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1904321.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23431..23835)
                     /gene="ybgC"
                     /locus_tag="OQQ66_001335"
     CDS             complement(23431..23835)
                     /gene="ybgC"
                     /locus_tag="OQQ66_001335"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1904322.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23985..24278)
                     /gene="ybgE"
                     /locus_tag="OQQ66_001336"
     CDS             complement(23985..24278)
                     /gene="ybgE"
                     /locus_tag="OQQ66_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1904323.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24278..24391)
                     /gene="cydX"
                     /locus_tag="OQQ66_001337"
     CDS             complement(24278..24391)
                     /gene="cydX"
                     /locus_tag="OQQ66_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1904324.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24406..25545)
                     /gene="cydB"
                     /locus_tag="OQQ66_001338"
     CDS             complement(24406..25545)
                     /gene="cydB"
                     /locus_tag="OQQ66_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1904325.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25561..27129)
                     /gene="cydA"
                     /locus_tag="OQQ66_001339"
     CDS             complement(25561..27129)
                     /gene="cydA"
                     /locus_tag="OQQ66_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1904326.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27977..>28703)
                     /locus_tag="OQQ66_001340"
     CDS             complement(27977..>28703)
                     /locus_tag="OQQ66_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1904327.1"
                     /translation="GSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMA
                     VFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLL
                     SCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKM
                     PPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEE
                     NQGSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         7254 a         7546 c         7006 g         6897 t
ORIGIN      
        1 aacagctata gcttctggtc ttgccgaaga agatccagct tctgcaacca ggccattcag
       61 aactgaagtg aagcgcagtc gactgttaat tgacgagtat ctgttaatca ggaaaatagc
      121 agacgtccag aacgaatggt tcggcctttg catggatctt gcactggtaa caggacagcg
      181 cgaaggagat ctggcggcta tgagatggga ggatataaga gatggcagac tatacgtgga
      241 acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt ccagattgaa
      301 tttaacactt accgatgtac tggataactt gaaaaaaata aacggtaaaa acgaaaagtt
      361 actcggaggg aagacagcca ggacaatcgc agcacagttc aggattgccc gagacacatc
      421 cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt tatccggtcg
      481 cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac atcgttcatc
      541 cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca tttagaatta
      601 atttcgaatg atttcgaaac accacttgta attaattgat tataaaggat taattcctaa
      661 gaggcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac agctaaaatt
      721 agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat agtggtatcg
      781 gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc atgaccacac
      841 gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc ctgcttcctt
      901 catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag
      961 gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat acacctgcta
     1021 tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc
     1081 caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac cacattcacg
     1141 gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat gtcattcgca
     1201 cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca caagtcgctt
     1261 ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg acgcacgatg
     1321 acatgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga ctggagtgtg
     1381 aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa ggtaaacgtt
     1441 tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg ttcggcgata
     1501 actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg ggcaacgccg
     1561 atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc gacagcattg
     1621 cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact tttaatttgc
     1681 gcgtacagcc acaggcctgg tttgatcgcc agttcatccc tggcccacgg gctgatacgc
     1741 gcccacagcg ttttaccgcc gacttccagc tccacttcca cctgaccgtt gtcgtcataa
     1801 ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa
     1861 accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag cggttcgtcc
     1921 agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga
     1981 tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg cagccacgga
     2041 ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac ctgaccgttt
     2101 tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg gctgacatac
     2161 aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag ttcgcgttta
     2221 cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg tgccgtcagc
     2281 aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg
     2341 tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc gaccatgctt
     2401 ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag ccgcgcatcc
     2461 tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt ttcggcatca
     2521 tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag tccactgatg
     2581 gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt gatgccattg
     2641 gcgggcagcg tttcattaat agtcaggcaa tggttgccca acgtctggga aaaattcagt
     2701 tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat tctgaaatca
     2761 acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg ccactttccc
     2821 cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt tcgccaggaa
     2881 tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca aaagccagta
     2941 ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc
     3001 agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc ccttccagcg
     3061 ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg agaaccgcgc
     3121 cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca ccgataaatc
     3181 cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt aacaccagcg
     3241 gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc accagtaacc
     3301 aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa actttcaggc
     3361 ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg taaatccgta
     3421 acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg ctttcactgt
     3481 cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat gtgaatcttc
     3541 cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac cgtaaacaat
     3601 gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat cttccgccgg
     3661 agccaatttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt cttttgcata
     3721 aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat tcagcagcga
     3781 agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt tcggtgctac
     3841 aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg cttttttatc
     3901 aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag gcgctcccgc
     3961 ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat ccacgccttt
     4021 ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa
     4081 caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag agagtgccgc
     4141 cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt aacgttatat
     4201 aggtgacaat ataacggcta actcagaatt ttcccagcag ttattgctaa cctttgtgag
     4261 gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa atgcccgact
     4321 aagtgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac caacaccaga
     4381 aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg ccatcacgac
     4441 aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg tcaactccag
     4501 atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct tttgtgcggt
     4561 cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac attgttatgc
     4621 aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac ccgcgccgga
     4681 tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg aaagatgccg
     4741 gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta agcgagcata
     4801 ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg acccgctatg
     4861 gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg
     4921 tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc tcacgttttt
     4981 cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc gatcatgatg
     5041 acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa
     5101 ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg ttgatattgc
     5161 taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac gccaacaacc
     5221 aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa gtattaatgg
     5281 cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc
     5341 agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc gccacgctgt
     5401 gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc gctgcacaaa
     5461 atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca
     5521 aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc gtttgtcggt
     5581 tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact tccgcttttg
     5641 aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga gcaattgcaa
     5701 aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc tggcgaagat
     5761 gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc accgcgttgc
     5821 atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt taaatacctt
     5881 tccactggcg agacgcgaaa aatcctgctg tgtcaggcgc tgatgtcgga gcctgacttg
     5941 ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca gctggctgag
     6001 cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa tcgcttcgat
     6061 gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt agcggaaact
     6121 ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcaca tagtgaacag
     6181 cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc cttacccgcc
     6241 aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg ccccattctt
     6301 aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg gccaaatggt
     6361 gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg ttacagcaac
     6421 gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga tatcaaaaag
     6481 catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac taccgtgcgt
     6541 aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt ttcggatcgc
     6601 cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg tacggctgac
     6661 gctccgttcc atagtctttc ctggggacag cagcatctgg cgctgatcgt ccgcgcactg
     6721 gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga tccgctcaat
     6781 cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg
     6841 tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct tgagttcgtg
     6901 ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg tagtgctgac
     6961 cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg caggcatgaa
     7021 accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt ttataatcac
     7081 ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa tttattccat
     7141 gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc ctaatggagc
     7201 gaattatgag agttctgctt accggtggta gcggttacat tggaagtcat acctgcgtgc
     7261 aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac agtaagcgca
     7321 gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata
     7381 ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac accgtgatcc
     7441 acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa tattacgaca
     7501 acaatgtcaa cggtactctg cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact
     7561 ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca tacgttgaaa
     7621 gcttcccgac cggcacaccg caaagccctt acggcaaaag caagctgatg gtggaacaga
     7681 tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg cgctacttca
     7741 acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc attccgaata
     7801 acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg gcgatttttg
     7861 gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac gtaatggatc
     7921 tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc gtacacatct
     7981 acaacctcgg cgctggcata ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag
     8041 cctgcggcaa accggttaac tatcattttg caccgcgtcg cgagggcgac cttccggcct
     8101 actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg
     8161 atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga tatcccgatt
     8221 aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc tacaacccgc
     8281 tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc
     8341 aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc ctctgcgcag
     8401 gtaatgtgcg ggtgacaggc gataaaaacc acgattacac cgggacttac gttttcacta
     8461 atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac gatcctttga
     8521 tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg gatcacagta
     8581 aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc
     8641 aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg
     8701 cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc ttcctgccta
     8761 acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa
     8821 tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg
     8881 aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg ctactgctgc
     8941 ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc
     9001 tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc ttcccctact
     9061 ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg cagctgcacg
     9121 cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg gttggttatg
     9181 aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg
     9241 cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa
     9301 cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt caggcgcctg
     9361 gccgcgtgaa tttgattggt gaacacacgg actacaacga cggtttcgtt ctgccctgcg
     9421 cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga
     9481 tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc attgtcgcgc
     9541 atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg caactgcgta
     9601 acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt
     9661 taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc
     9721 tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac cagtttgttg
     9781 gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa gatcatgcct
     9841 tgctgattga ctgtcgttca ctggggacca aagcagtttc catgccgaaa ggtgtggctg
     9901 tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac aacacccgtc
     9961 gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc gatgtcacca
    10021 ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa cgcgtgcggc
    10081 atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag cagggcgacc
    10141 tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa
    10201 tcaccgtgcc gcaaattgac actctggcag aaatcgtcaa agctgtgatt ggcgacaaag
    10261 gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg atcccggaag
    10321 agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa acaggtatta
    10381 aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga acgaaactcc
    10441 cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca acgcagggat
    10501 ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc cgctttccga
    10561 tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc aggatcaggc
    10621 cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata gccgttatac
    10681 ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc tgcacggcgg
    10741 cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc gtcaggtgct
    10801 gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg gcgcaacggt
    10861 gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca cagttgataa
    10921 accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg agcagtctga
    10981 cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg atgaaggcgg
    11041 cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa
    11101 aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag gttacgatca
    11161 cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc
    11221 agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat tctactccgg
    11281 caacttcctc ggcggcacac cgtcgcgggg aacagaacct tacgccgact ggcaagggct
    11341 ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc cgcaaccgga
    11401 ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt ttattgctga
    11461 gtaatgttaa gccctcgtca tgagggcttt atctcatatt gctcaaatca ccagcaaaca
    11521 ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta ttttcgctat
    11581 ggttatgcgt aagcattgct gttgcttcgt cgcggcaata taatgagaat tattatcatt
    11641 aaaagatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg ttcgtcatgg
    11701 cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg atctgtctga
    11761 gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag gttacagctt
    11821 tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga atgtgctgga
    11881 cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg aacgtcacta
    11941 cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg acgagcaggt
    12001 gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag atgatgagcg
    12061 ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaactgc cgctgacgga
    12121 aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta ttctaccgcg
    12181 tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc gtgcgctggt
    12241 gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc cgactggcgt
    12301 gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt atctgggtaa
    12361 tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag cgaagtaaac
    12421 gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg ttgctaacaa
    12481 tggagcacat tgcctgatgc gacgcttcgc gacttatcag gcctgtggtg attcatcgga
    12541 tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca
    12601 ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc atcggtgatg
    12661 ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc caccagatgg
    12721 ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca
    12781 gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct gaaatcgatc
    12841 atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc aacgtgcttc
    12901 gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccgctg
    12961 gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg cggcgcaccg
    13021 gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt tttgaagccg
    13081 actggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt ggtacgtgcg
    13141 ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat atcgagaaac
    13201 tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacga
    13261 agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcag ccctttccag
    13321 cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc
    13381 agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga
    13441 attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg
    13501 atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg gaatttttcc
    13561 agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga
    13621 taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct
    13681 atatcttccg tcagaatgtg taaacggggt tttacattat gaacggatta atcctggaat
    13741 tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt
    13801 aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa
    13861 tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat
    13921 aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg
    13981 ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata
    14041 acggccaggc aaacgccgca actgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg
    14101 tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc
    14161 attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca
    14221 ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca
    14281 tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca
    14341 ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc
    14401 ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac
    14461 gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac
    14521 tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct
    14581 ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt
    14641 gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac
    14701 gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga
    14761 ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga tggcaattgc
    14821 cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga
    14881 gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt tgggttcggt
    14941 tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg ctgaccccat
    15001 tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag
    15061 cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg
    15121 tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg
    15181 tgagaagccg gtgatgacgc tgcatgtgca ggtgatcccg ccacacgctc acgatgcctt
    15241 gttggatcag atccaacact acctgatgga tcactatcag attgagcacg ccaccattca
    15301 gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg tgtctggcca
    15361 ttcacatcat caccattaat gggacagcgc gcatgagcct ctttcacgtg cgctgttgat
    15421 ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc
    15481 gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag
    15541 ccagttttcc acatacttac gcgtcatcag aagcattgcc acgatagata acaccatcat
    15601 gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc
    15661 taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa
    15721 taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg ccttcggcaa
    15781 tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata
    15841 ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg
    15901 aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga agtagttgct
    15961 gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc
    16021 gatccatgag agatcataac cgcctgctcc tattggtata tgtaccagga tattctgcac
    16081 actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac
    16141 gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc
    16201 tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct gcgatggcct
    16261 gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt gcaccctcac
    16321 ctgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa
    16381 taccccgatc ggttgccaca ataagcctct gatgtggcaa tgttttcgca gcagcgatca
    16441 gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat
    16501 gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa
    16561 attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc gtctgttttt
    16621 gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa
    16681 gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta
    16741 ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca tcgcaaaatg
    16801 cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga agtgtcggca
    16861 tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag
    16921 cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag
    16981 aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg
    17041 caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt
    17101 tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat
    17161 caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag
    17221 cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg
    17281 tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa
    17341 gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag
    17401 cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt
    17461 gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt tacctgaaag
    17521 atttctactc ggggccaatc tttaagattg gtgtgtcgtg caggatgact cgcttcgctt
    17581 gccctacggg ccgttgctgg cgcaacgtta tccttcacgc ttaacatctg agtttgatgt
    17641 taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    17701 ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc gccgaccccc
    17761 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgataatgcg ctggatacta
    17821 caaactactt ccaccgaacc catcgtaatt agtggtgggt cgtgcaggat tcgaacctgc
    17881 gaccaattga ttaaaagtca actgctctac cgactgagct aacgacccgg tggtgggtga
    17941 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagccaa
    18001 tcacccaata ctacgctgga tactacaact aactatgtta gtggtgggtc gtgcaggatt
    18061 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact
    18121 ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt
    18181 cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga
    18241 cacgaccaga aataatgcaa cttctggtcg tgtgttatgc attacatcgc gttcagacgt
    18301 ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg
    18361 gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca
    18421 tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca
    18481 tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg
    18541 taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg
    18601 cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt
    18661 ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgcc
    18721 gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg
    18781 atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc
    18841 tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt
    18901 tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg
    18961 accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca gggggccgct
    19021 ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc
    19081 tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt
    19141 actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta
    19201 ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc
    19261 ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc
    19321 aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc
    19381 agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct
    19441 tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg
    19501 ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca
    19561 ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta
    19621 ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc
    19681 agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc
    19741 catcccctga gaagagctgt agattaccat agtgccatta ggtgccagac ttggcgtttc
    19801 atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat
    19861 gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc
    19921 cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt
    19981 cactttataa acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc
    20041 cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc
    20101 agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag
    20161 tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg
    20221 acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt
    20281 cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga
    20341 acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa
    20401 ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc
    20461 ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt
    20521 gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag
    20581 ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga caactacagc
    20641 gtcaatgccc agtgcggacc atgcagatgg ttgtacttcc tgcgcactac ccggctgctg
    20701 tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac
    20761 gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc
    20821 aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac
    20881 tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc
    20941 cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa
    21001 aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg
    21061 cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg
    21121 tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc
    21181 gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt
    21241 acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat
    21301 ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag aattaccact
    21361 cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc
    21421 agagcttagc tcaccgaaaa tatcatctgc ctctgcggcg gctttttctg ccgctgcttt
    21481 cttatcagct gctgcctttt cagcagccgc tttttctgct gcttttttgt cggctgcagc
    21541 tttcttatca gctgcagcct tttcagcagc cgctttcttc tcagcttctg ctttggcttt
    21601 ttcagcagct tcagttgccg ctttctttct tgcttcagct gcagctgctt ctgccgcttc
    21661 cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctg cggctttggc
    21721 ggcttctgct tctgcttttt tctttgcgtc tgcagccgct ttcttcgctg cttcttccgc
    21781 agctttagca tctgcttcgg ccttcgcttt agcatctgcc gccgctttcg ctgccgcctc
    21841 ttcagcttgc ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg
    21901 ctcctgagcc gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg
    21961 tttctcacgc agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg
    22021 cttcgcgctt gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc
    22081 aaccatgaca gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc
    22141 atcgaacgaa ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat
    22201 tatcgcccgc ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa
    22261 actgttcgcc tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct
    22321 gcgtcattaa accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta
    22381 tttcatcgta aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct
    22441 tgaagcggct ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt
    22501 tctcaaccac cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat
    22561 cgttactgct caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg
    22621 tgatgatggg cgctgtcgcc atgaagatca gcaacagcac cagcagtacg tccagcaacg
    22681 gtacaatgtt gatttcggac ttaagttcgc gacgacctcg tccacgcgct ctggccatga
    22741 cttacccctt attgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact
    22801 cttccataaa gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg aggcggttgt
    22861 aggccataac tgccgggata gcggcaaaca gaccaattgc agtcgcaatc aacgcttctg
    22921 cgataccggg cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg
    22981 catgcatgat cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg
    23041 taccgaggaa cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca
    23101 tagcacgcga cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc
    23161 gcacaaactc tttgaacccg ctgtaaaata tttgttccga accagtcaga ttatcccgtt
    23221 tcccctgact ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa
    23281 acgcttcggc ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata ataatggccc
    23341 aggatgcgat tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct
    23401 tcaggaacaa atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt
    23461 gggaagcgca cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc
    23521 attcagcaaa gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg
    23581 cattgatgtt atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata
    23641 ttccaccgtc attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact
    23701 gaagtgatga tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc
    23761 gtggtacacc acaccaccgg catcggtatc ttcatagtag acgcgagccg gccatcgaaa
    23821 cagcgttgta ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg
    23881 ctactatacg cgcgcgattg aggtttggga atgagggcaa gttaagggag cgtaaaaatt
    23941 tatgggcctg gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa
    24001 atcagcccga taatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga
    24061 accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc
    24121 aataaaccat gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa
    24181 aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg
    24241 tccattaccg catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt
    24301 tgcctgattc gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa
    24361 gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg
    24421 ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc
    24481 ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt
    24541 cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg
    24601 catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga
    24661 ggagaacaca aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcgg
    24721 cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag
    24781 ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc
    24841 cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc
    24901 gaaacagacc agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag
    24961 ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct
    25021 caccacgcct gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta
    25081 cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgcaac
    25141 accaattacc agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg
    25201 ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga
    25261 cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg
    25321 ccaggcagca aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg
    25381 tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt
    25441 gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc
    25501 aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc
    25561 ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag
    25621 gctgcttggg ccgaggcgtg caaatttgaa cattaagaac aattctgcca ccaggaacag
    25681 ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga
    25741 attcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc
    25801 cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg
    25861 cagcagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat
    25921 gattgccagc agcaggaagc cacacgccac cataatacgg aacgcgaagt acagcggcgc
    25981 tacacgcgga atggagtctt tagttgcctg ttgaatctgc gcttcagtcg catcagccac
    26041 gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa
    26101 ctggtcacga accgcctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc
    26161 cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac
    26221 cggggtatcc acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa
    26281 tttgttcgtc tcctcttcct gatcaggaat gccgaacaga gtaaaggcag caggcgcagg
    26341 ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc
    26401 gtagccggat tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc
    26461 agcgatagca aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc
    26521 gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa
    26581 tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat
    26641 agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat
    26701 ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac
    26761 tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa
    26821 ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata
    26881 gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc
    26941 gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc
    27001 ggagaggacg tagaccgttt ccataatggc cagcaggaac gccataccga gcgtcagtgg
    27061 cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat
    27121 atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa
    27181 agggtcacac gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat
    27241 gcacacaaaa acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc
    27301 cacgtatata ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg
    27361 catttttcga cggtgatcaa tttatagcta aattaccgcc tttcagccaa tttgatcgag
    27421 aacaatttat ctctttttga tgcccatttc caagacttat acattgataa atatcaattc
    27481 ctacaaatta tgttaatcat gtttcaataa ggtaacttta gtgacattta tgtttaaaat
    27541 gtgtgagtta taaggctgaa attagtttcg gttggcaatt aaagataaaa agagacgaag
    27601 caaaaacagc acggttgacc ttaattcttt ttttggtcac tggctaatac gacgcagagt
    27661 ttcaggatta ctaataaagg cataaatcca cccccgccga tattaacatt taattaactt
    27721 ataattatat tataattaac tcacccaggg ttattatttt gttaaaaata aagccacata
    27781 tacgtggctt gagagtgaag ataaacgcaa aattacctga tgcgctacgc ttgccagtgt
    27841 ctacatcatc tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca
    27901 tgccgtatcc ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct
    27961 tcggtttgat tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa
    28021 aaggccccga tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg
    28081 tttctgagca agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt
    28141 cagccggatt aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc
    28201 ttatcaggca tcccactggg ggcattttca acaaactata actttccggc acgttgaatc
    28261 cggctttgtt gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca
    28321 gccatgctcg cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata
    28381 aacttaggcg acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc
    28441 ccgaaggccg cccaggcctt aaaagcagat cttctttgcc cagtaagccc acgccacgca
    28501 gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtaaccctt
    28561 cgctaaagac agccatgccg ttacgccctt cctgtaaggc aacatagttg agcatattcc
    28621 ataccggaac cggcgcttct ttccagcctt cttgctgcca gttgttcatt gcactgtcgt
    28681 tcacggggcg cgttagcgaa cca
//
LOCUS       DAJVTY010000033        28677 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVTY010000033 DAJVTY010000000
VERSION     DAJVTY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28677
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.033"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            35..802
                     /gene="hdhA"
                     /locus_tag="OQQ66_001341"
     CDS             35..802
                     /gene="hdhA"
                     /locus_tag="OQQ66_001341"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS1904328.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            1031..1609
                     /gene="uidR"
                     /locus_tag="OQQ66_001342"
     CDS             1031..1609
                     /gene="uidR"
                     /locus_tag="OQQ66_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1904329.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERAREPIEGIHFVDYMVESIVSLTHEAFGQRALVV
                     EIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLLDLTY
                     GVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1997..3797
                     /gene="uidA"
                     /locus_tag="OQQ66_001343"
                     /pseudo
     CDS             1997..3797
                     /gene="uidA"
                     /locus_tag="OQQ66_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            3794..5167
                     /gene="uidB"
                     /locus_tag="OQQ66_001344"
     CDS             3794..5167
                     /gene="uidB"
                     /locus_tag="OQQ66_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1904330.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVACVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5206..6471
                     /gene="uidC"
                     /locus_tag="OQQ66_001345"
     CDS             5206..6471
                     /gene="uidC"
                     /locus_tag="OQQ66_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1904331.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6697..8205)
                     /locus_tag="OQQ66_001346"
     CDS             complement(6697..8205)
                     /locus_tag="OQQ66_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1904332.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8306..9481)
                     /gene="manA"
                     /locus_tag="OQQ66_001347"
     CDS             complement(8306..9481)
                     /gene="manA"
                     /locus_tag="OQQ66_001347"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1904333.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9680..11326
                     /gene="fumB"
                     /locus_tag="OQQ66_001348"
     CDS             9680..11326
                     /gene="fumB"
                     /locus_tag="OQQ66_001348"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1904334.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            11469..12872
                     /gene="fumC"
                     /locus_tag="OQQ66_001349"
     CDS             11469..12872
                     /gene="fumC"
                     /locus_tag="OQQ66_001349"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1904335.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(12869..13798)
                     /gene="tus"
                     /locus_tag="OQQ66_001350"
     CDS             complement(12869..13798)
                     /gene="tus"
                     /locus_tag="OQQ66_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1904336.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13874..15175)
                     /gene="rstB"
                     /locus_tag="OQQ66_001351"
     CDS             complement(13874..15175)
                     /gene="rstB"
                     /locus_tag="OQQ66_001351"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1904337.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(15179..15898)
                     /gene="rstA"
                     /locus_tag="OQQ66_001352"
     CDS             complement(15179..15898)
                     /gene="rstA"
                     /locus_tag="OQQ66_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1904338.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            16024..16347
                     /locus_tag="OQQ66_001353"
     CDS             16024..16347
                     /locus_tag="OQQ66_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1904339.1"
                     /translation="MKAALGVLVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHYIVAS
                     ERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWVLIIC
                     WIKLH"
     gene            complement(16344..17066)
                     /gene="folM"
                     /locus_tag="OQQ66_001354"
     CDS             complement(16344..17066)
                     /gene="folM"
                     /locus_tag="OQQ66_001354"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1904340.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17103..18485)
                     /locus_tag="OQQ66_001355"
     CDS             complement(17103..18485)
                     /locus_tag="OQQ66_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1904341.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(18671..19615)
                     /locus_tag="OQQ66_001356"
     CDS             complement(18671..19615)
                     /locus_tag="OQQ66_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1904342.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            20138..21670
                     /gene="pntA"
                     /locus_tag="OQQ66_001357"
     CDS             20138..21670
                     /gene="pntA"
                     /locus_tag="OQQ66_001357"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1904343.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            21681..23069
                     /gene="pntB"
                     /locus_tag="OQQ66_001358"
     CDS             21681..23069
                     /gene="pntB"
                     /locus_tag="OQQ66_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1904344.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23094..23999)
                     /gene="tqsA"
                     /locus_tag="OQQ66_001359"
                     /pseudo
     CDS             complement(23094..23999)
                     /gene="tqsA"
                     /locus_tag="OQQ66_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            24176..25405
                     /locus_tag="OQQ66_001360"
     CDS             24176..25405
                     /locus_tag="OQQ66_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1904345.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFIYRAGTGRETRPERIKLGSYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            25772..25960
                     /locus_tag="OQQ66_001361"
     CDS             25772..25960
                     /locus_tag="OQQ66_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1904346.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            26018..27040
                     /locus_tag="OQQ66_001362"
     CDS             26018..27040
                     /locus_tag="OQQ66_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1904347.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHTCNERFSLYSLRNCSRAKAHGAN
                     LSDSCSIFLRRLFRAGDNVLVNSLSVIVLSCIAMRRNTLHHGADGDYSGSLALRRWRY
                     STSRFNAAVTNCPVLSPGIFTASTLFITSCGTLAATVCDFAFTAFVAMWCTPHKKQMQ
                     YAGKKISVQHLTCLTPGFKLVFNTLLAQGAETTKPGSALTLTGLLTTNDSESIEVAMR
                     NHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEARQQSPAGCVMVFAARI
                     RQGVGL"
     gene            27037..27267
                     /locus_tag="OQQ66_001363"
     CDS             27037..27267
                     /locus_tag="OQQ66_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904348.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHVQ"
     gene            27257..27481
                     /locus_tag="OQQ66_001364"
     CDS             27257..27481
                     /locus_tag="OQQ66_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904349.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDISVIADRAHKHAARESECIFH
                     YLTFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            27474..28052
                     /locus_tag="OQQ66_001365"
     CDS             27474..28052
                     /locus_tag="OQQ66_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904350.1"
                     /translation="MHNLSISDLNSIQFDEKFTGQLLVNVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPSTSHHDDDLHFTGCMVSHYENGVEVSCERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGVNMRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTR
                     EYKSTGLDVLNIVADYADFVTEVILRKTTDGD"
     gene            28054..28287
                     /locus_tag="OQQ66_001366"
     CDS             28054..28287
                     /locus_tag="OQQ66_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904351.1"
                     /translation="MRDIYLETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            28293..28592
                     /locus_tag="OQQ66_001367"
     CDS             28293..28592
                     /locus_tag="OQQ66_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1904352.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
BASE COUNT         7363 a         6813 c         7403 g         7098 t
ORIGIN      
        1 cttttatttt tactgaagtt cacaggaggt ttatgtgttt aattctgaca acctgagact
       61 cgacggaaaa tgcgccatca tcacaggtgc gggtgcaggt attggtaaag aaatcgccat
      121 tacattcgcg acagctggcg catctgtggt ggtcagtgat attaacgccg acgcagctaa
      181 ccatgttgta gacgaaattc aacaactggg ggggcaggca tttgcctgcc gttgtgatat
      241 tacttccgaa caggaactct ctgcactggc agactttgct atcagtaagc tgggtaaagt
      301 tgatattctg gttaacaacg ccggtggcgg tggacctaaa ccgtttgata tgccaatggc
      361 ggattttcgc cgtgcttatg aactgaatgt gttttctttt ttccatctgt cacaacttgt
      421 tgcgccagaa atggaaaaaa atggcggtgg cgttattctg accatcactt ctatggcggc
      481 agaaaataaa aatataaaca tgacttccta tgcatcatct aaagctgcgg ccagtcatct
      541 ggtcagaaat atggcatttg acctgggtga aaaaaatatt cgggtgaatg gcattgcgcc
      601 gggggcaata ttaaccgatg ccctgaaatc cgttattaca ccagaaattg aacaaaaaat
      661 gttacagcac acgccgatca gacgtctggg tcaaccgcaa gatattgcta acgcggcgct
      721 gttcctttgc tcgcctgcag ccagctgggt aagcggacaa attctcaccg tctccggtgg
      781 tggggtacag gagctcaatt aatacactaa cgaaccggta aacagccgta tgtgttgttt
      841 accgggatag cgtgtgcaat tcatcaacgt ctctgctaaa caactggcag ccaaatcacg
      901 gctattggtt aaccaatagc tggatgaaaa gcatacgaat agagtgtgcc ttcgcactat
      961 tcaacagcaa tgataggcgc tcacctgaca acgcggtaaa ctagttattc acgctaacta
     1021 taatggttta atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct
     1081 caatgctgcc agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat
     1141 ctgtaaatcc tgcgccatta gtcccgggac gctttatcac catttcatct ccaaagaagc
     1201 cttgattcag gcgattatct tacaggacca ggagagggcg cgggaaccga ttgaagggat
     1261 tcattttgtt gactatatgg tcgagtctat tgtctctctc acccatgaag cctttgggca
     1321 acgggcgctg gtggtcgaaa ttatggcgga agggatgcgt aacccacagg tcgccgccat
     1381 gcttaaaaat aagcatatga cgatcactga atttgttgcc cagcggatgc gtgatgccca
     1441 gcaaaaaggc gagataagcc cagacatcaa cacgtcaatg acttcacgtt tactgctgga
     1501 tctgacctac ggtgtactgg ccgatatcga agcggaagat ctggcgcgtg aagcgtcgtt
     1561 tgctcaggga ttacgcgcga tgattggcgg tatcttaacc gcatcctgat cctctcattt
     1621 cgcgggctgg tgataactgt gcccgcgttt catactttaa tttccctgta caaaatacaa
     1681 tctagccggg atcggagaat tcccccccaa aatattcacc gtagccatat gtcatgagag
     1741 tttatcgttc tcaagatgct cgaacgagcg ttcgattgct tattttatgg cttctgtcaa
     1801 cgctgtttta aagattaatg cgatctatat cacgctgtgc gtattgcaga ttttggtttt
     1861 ttgatcgcgg tgtcagttct ttttatttcc atttctcttc catgggtttc tcacagataa
     1921 ctgtgtgcaa cacagaattg gttaactaat cagattaaag gttgaccagt atttttattt
     1981 gaatgaggag tcccttatgt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
     2041 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
     2101 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
     2161 cgccgatgca gatattcgta attatgtggg caacgtctgg tatcagcgcg aagtctttat
     2221 accgaaaggt tgggcgggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
     2281 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
     2341 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
     2401 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgatg aaaacggcaa
     2461 gaaaaagcag tcttacttcc atgatttctt taactatgcc gggatccatc gcagcgtaat
     2521 ggtctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgttgcgca
     2581 agactgtaac cacgcgtctg ttgactggca ggtggtagca aatggtgatg tcagcgttga
     2641 actgcgtgat gcggatcagc aggtggttgc aactggacaa ggtaccagcg ggactttgca
     2701 agtggtgaat ccccacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
     2761 agccaaaagc cagacagagt gtgatatcta cccgctgcgc gtcggcatcc ggtcagtggc
     2821 agtgaaggac gaacaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg
     2881 cggatttgcg aggcaaagga ttcgataacg tgctgatggt gcacgatcac gcattaatgg
     2941 actggattgg ggccaactcc taccgaacct cgcattaccc ttacgctgaa gagatgctcg
     3001 agtgggcaga tgaacatggc atcgtggtga ttgatgaaac tgcagctgtc ggctttaacc
     3061 tctctttagg cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg
     3121 ctgtcaacgg ggaaactcag caggcgcatt tacaggcgat taaagagctg atagcgcgtg
     3181 acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc
     3241 aaggtgcacg agaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc
     3301 gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc
     3361 tctttgatgt gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg
     3421 aaacggcaga gaaggtactg gaaaaagaac ttctagcctg gcaggagaaa ctgcatcagc
     3481 cgattatcat caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg
     3541 acatgtggag tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc
     3601 gcgtcagcgc cggcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag
     3661 gcatattgcg cgttggcggt aacaagaagg ggagcttcac ccgcgaccgc aaaccgaagt
     3721 cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc
     3781 agggaggcaa acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg
     3841 tgacgtcgcc aataacttcg ccttcgcaat gggggcgctc ttcctgttga gttactacac
     3901 cgacgtcgct tgcgtcggtg ccgctgcggc gggcaccatg ctgttattgg tgcgggtatt
     3961 cgatgccttc gccgacgtct ttgccggacg agtggtggac agtgtgaata cccgctgggg
     4021 aaaattccgc ccgtttttac tcttcggtac tgcgccgtta atgatcttca gcgtgctggt
     4081 attctgggta ccgaccgact ggagccatag cagcaaagta gtgtatgcat atttgaccta
     4141 catgggcctc gggctttgct atagcctggt gaatattcct tatggttcac ttgctaccgc
     4201 gatgacccaa caaccacaat cccgcgcccg tctgggcgcg gctcgtggga ttgccgcttc
     4261 attgaccttt gtctgcctgg catttctgat agggccgagc attaagaact ccagcccgga
     4321 agagatggtg tcggtatacc atttctggac gattgtgctg gcgattgccg gaatggtgct
     4381 ttacttcatc tgcttcaaat cgacgcgtga gaatgtggta cgtatcgttg cgcagccgtc
     4441 attgaagatc agtctgcaaa ccctgaaacg gaatcgcccg ctgtttatgt tgtgcatcgg
     4501 tgcgctgtgt gtgctgattt cgacctttgc ggtcagcgcc tcgtcgttgt tctacgagcg
     4561 ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa acttggttgg
     4621 tactgtggca tcggcaccgc tggtgccggg gatggtcgcg aggagcggta aaaagaatac
     4681 cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct gggtttccgt
     4741 ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc agggcgttac
     4801 tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat acctgaccgg
     4861 cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat gcggtcaggc
     4921 aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg ccaatcaggt
     4981 gcaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac cttgcggatt
     5041 tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa aattcaaaga
     5101 aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca gcgatatcac
     5161 taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag aaaaatagtg
     5221 gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta tgcggcgcaa
     5281 ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg cagggcggat
     5341 aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagagagg attgttcgat
     5401 ttcaatagtg gttattattc caatattatt ggcgttgaag gcggggcata ttatgtttat
     5461 aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga taaaagtttt
     5521 ggctttgccc tgggggcagt aaaaataaaa ccctgtgaaa atagcctgct taaattaggt
     5581 cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt aatggctggc
     5641 agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg ggctttaaca
     5701 ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac tgattcaaca
     5761 accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga taaacatcgc
     5821 gcacgttctt ttttagccgc cagttggcat gatgatacca gtcgctattc tctgggggca
     5881 tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag cataccgctc
     5941 gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct cgaaggttta
     6001 agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt gacctggaat
     6061 gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat gaatggtgcc
     6121 gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa ccgtgaagga
     6181 atgcagtcct ggcaattggg cgtcaactat cgtttaacgc cgcaatttac actgacattt
     6241 gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat tgaaggcacg
     6301 ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg gatgaatttc
     6361 tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg cgatcgcctg
     6421 aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta agaaataacg
     6481 ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt tttatgccgg
     6541 atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt attgcaggaa
     6601 aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc agatgaaaac
     6661 gccgaagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa ttgccggtac
     6721 agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta acgacatttt
     6781 ctgcccattt aacgttatct gaccgttagt atattgcagg ctggtggtga tggtattgtc
     6841 ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa cttgctgttt
     6901 cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct gagtcataaa
     6961 ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa ccgaacgatc
     7021 tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt tcaggaacag
     7081 cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg cgacagtaat
     7141 caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg tcactttctc
     7201 ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc
     7261 gttatattgc tggctgaact gatgccaggc ttcaccatca atctggccga cttttaaagt
     7321 cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg agtaatccag
     7381 ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga tctccatgcc
     7441 ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca gtgacagttt
     7501 ttggtttcct acgcgctcac caaaacttgc cagcgtgctg gaaccgtcgg ttttcagatt
     7561 attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc gaccactttg
     7621 cgcctccccg gaaagggaga tggctttgcc gtctctgtca gcatttaact ggaactcgcc
     7681 gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggcttga gcgaaatatc
     7741 ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg tttcaccttt
     7801 tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt gaattgatgc
     7861 catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac catgatcaac
     7921 cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt tcccggcaat
     7981 gggtttcacc aacagttgca actgactgct gaatacgccg cgatgatagt tttgataact
     8041 cacttccagg ttggattcag gagctgtcag ttttagttgc gcgttcgcct gcgcgaccat
     8101 gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg tccagactac
     8161 gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga ttatccataa
     8221 aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa gcttagcaag
     8281 agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct aaacggccgt
     8341 ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca cccggtttaa
     8401 gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag aacaaaatgg
     8461 cggcactctg ctggctaatg gtggtttctt tatcactaag gtcatgcagc gagaaggcaa
     8521 aatcatccac tggaatcggg aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca
     8581 actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga atatcaatgt
     8641 acttaggcgt cagacccgca cgcagcacgt tatcggagtt cgccatcact tccagcgcca
     8701 cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg ccaggattca
     8761 atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg taaaattcag
     8821 aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc gattttaaaa
     8881 tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggctggcg aacagttcgc
     8941 ttaaacgttc ggcattaggc tgttgtaaaa agtgagcaat tgccggatgt gcacctgcga
     9001 ctggctggag tagggagaca atctcggaaa actcacgaaa cgcgttcatc gcaaggaaag
     9061 gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta cgctcggcgg
     9121 catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa ttgcgtttgt
     9181 ttggatgaac ctgaatggag agcggctgtg ctgcgcataa tactttgaac aggaaaggca
     9241 gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca ctctcaatca
     9301 catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg cttttcggat
     9361 gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata ccataaagtt
     9421 cagtcaacgc cgttttgctg ccccaggcat agttttgcac tgagttaatg agtttttgca
     9481 tgatcaatcc ctgttttaat gtggaaatta atcccactat taaagcaaga atcctacggg
     9541 aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa aagtgtgtag
     9601 gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat aaccaaacca
     9661 ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcaggctc cttttccact
     9721 caaaaaagat gatactgagt attacctgct aaccagcgaa cacgttagcg tatctgaatt
     9781 tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg cgcgtcaggc
     9841 gtttcatgat gcgtcattta tgctgcgtcc ggctcaccaa caacaggtgg ccgacattct
     9901 gcgtgacccg gaagccagcg aaaatgataa atatgtggcg ctgcaattcc tgcgcaactc
     9961 cgacatcgcg gcgaaaggtg ttctgccaac ctgtcaggat accggcactg cgattattgt
    10021 tggtaaaaaa gggcagcgtg tatggaccgg tggctgtgat gaagcggcgc tggcgcgcgg
    10081 tgtctataac acttatatcg aagataattt gcgctactcg caaaacgcgc cgctggatat
    10141 gtataaagag gtgaataccg gcaccaatct gccagcgcag atcgatcttt atgccgttga
    10201 tggcgacgag tacaaattcc tctgtatcgc caaaggtggt ggttcggcaa acaagacgta
    10261 tctctatcag gaaaccaaag cgttactaac gccggggaaa ctgaaaaatt acctggttga
    10321 gaagatgcgc acgctgggta cggcggcctg tcctccgtat catattgcgt tcgtaattgg
    10381 tggaacttct gcagaaacta accttaaaac agtgaaactg gcttccgcta aatactatga
    10441 cgaactgcca acggaaggga atgagcacgg tcaggcattc cgcgatgtgg aactggaaaa
    10501 agaattgctg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg gtaaatactt
    10561 cgctcacgac atacgcgtga ttcgcctgcc acgtcacggc gcatcctgtc cggttggtat
    10621 gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc aggggatctg
    10681 gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc gtaaagcggg
    10741 agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc tcgcacagtt
    10801 gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg tcggtcgtga
    10861 tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc cgcagtacat
    10921 taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt atgcctccgg
    10981 ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac tgcaagcgca
    11041 gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga cggatgcctg
    11101 taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg tattggcgca
    11161 gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg aagccatctg
    11221 gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag gaaatgactt
    11281 cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag agccgccctt
    11341 cggggcggtt tttttacatg gcacgaaaga ccaaacactt gttatcaaat agtaaataat
    11401 aagtgagcta aaagttgctt agcgaaagca aaacagaaag aaaaaattaa acaggtgagg
    11461 agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt gatgtcccgg
    11521 cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc atttcgacgg
    11581 agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca gcggcaaaag
    11641 ttaatgaaga tttaggcttg ttgtctgaag agaaagcgag cgccattcgg caggcggcgg
    11701 atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg cagaccggct
    11761 ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc agtgaattac
    11821 tcggcggcgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg aacaaaagcc
    11881 aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg gcgctgcgca
    11941 agcaactcat tccgcagctt aaaaccctga cgcagacact gagtgaaaaa tcgcgtgcat
    12001 ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg ctaacactag
    12061 ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat atcgaataca
    12121 gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact ggactaaata
    12181 cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc tgtgcaccgt
    12241 ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg gttcaggcgc
    12301 acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat gtccgctggc
    12361 tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat gagccgggca
    12421 gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc atgctctgct
    12481 gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt aactttgaac
    12541 tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc ttgctggcag
    12601 atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat cgtgagcgaa
    12661 tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc cacattggtt
    12721 atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc ttaaaagctg
    12781 cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta cggccagaac
    12841 agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc agccgtggaa
    12901 tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg tcggcatcgt
    12961 aatttaacaa ctcaccaacg tccggcacgc ccgcaccatt atcccgatta atcagtgcaa
    13021 tcagcggtgt agggcaggcg tgttgcactt gtttttgatc tcctttgtac cagacgcggg
    13081 caatcggctg caccttcacc ggacgtttga tttttaactt cgcgttctgt ggcagggcag
    13141 cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc cacggtgcga
    13201 cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca cgatgcaaat
    13261 ttttaatgat atgtttatta gcccagccaa aacgtaaagt ggcggggtcg tgcagaacgg
    13321 tgagcgagcg gtaagcatta agggtgatca gccccggcaa atgacgatgc acccattcaa
    13381 aacgtgccgc ggtggggagt tctgactcaa ccgtgacgat atgctcgaac gtggtcttga
    13441 gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta tcgacctggt
    13501 aacacaacac gccaggcaga cggacagcgg ctttgctgct gcgattttcg gactgttgtt
    13561 gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg ccgagatgtt
    13621 gtttcacctc aatacgatta agcggattat gctcatcctc tttttttacc tccggcaaag
    13681 agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat gcagccagct
    13741 cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa cgcgccataa
    13801 tattaaccac tttagttaca acatccttaa tttatagcac aatggtggtg acttgaccat
    13861 cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac ggccagctaa
    13921 acgagaagcg agcaccaccc agttcgctgg tgtcacaatt aaccgtaccg cccattgcca
    13981 gtgctataga gtggacaatt gccagcccca gcccgcagcc gccggttgag cgatcccggc
    14041 tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg gcaatccctg
    14101 ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg ctggtttcaa
    14161 ccgttgaatg gcagtaccgc agggcgttat tgagcaaatt atccagcacg cgctccatta
    14221 agcgcatatc caacgccgca taatggcctt gcacgagcgt ttttatccgt accgttttat
    14281 cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc aggtctggtt
    14341 cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc agctcttcaa
    14401 ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggactcggcg gcgctcaggt
    14461 tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt tcgtgagcga
    14521 taccgtcaat aagctgtttt ttgctggcaa tcaaggcgtt gatattgtcc gccatctggt
    14581 taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata cgctcataga
    14641 gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc tgccagtgcg
    14701 gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg atcagggcga
    14761 tatccagcaa tcgcatctga tggagataat aaagataagg aacaggacca actgccagca
    14821 cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg gcgacaattt
    14881 cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc agtggctcga
    14941 cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc cagtcgtgtg
    15001 gggggatctc acgcaattcg ctgcgcatca gatacagcga actgttcatc aaatcatcca
    15061 gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac caacccaacc agcagagaca
    15121 tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacagtttt ttcatcgctt
    15181 attcccatgc atgaggcgca aaaagatagc ctttgttacg cacagtttta atgcgataag
    15241 gttctgcggc gttatcgagc agtttttttc ttaaccgcga aatagccacg tccacgttac
    15301 gatccagtcc gtcataactg acgccgcgta aatttttcag caatgcatcg cggtccatga
    15361 tttgcccggc atgggtagct aattcccaca ataattcgaa atcagctgtc gagagcgaga
    15421 tttcagtgtt agccagggtg actacgcggt tgatgggatc gatggtcaac gtgccgaaat
    15481 gcagggcttt gtagggagtc agagacgttt cctgaagacc tttggtcagt gtggcttgct
    15541 cattctgacg caaatgcaaa cgtaaacgcg ctagcaaaac agcagggggc atcgttttga
    15601 gaatatagtc gcaggcaccc atttccagtg ccaggatgtg gttcatatcg ctatcgagag
    15661 aggttagaag aacaatcggt ccagaccact ttgcgcgtaa atcacgacaa atggtaatgc
    15721 cgtccttgcc tggtagcatg atgtcgagta acaccaaatc cggattttct cgcaaaatgg
    15781 tttcttcggc ctggtcgccg cgcggctcta cggtaacctg catatcatgt tttgccaggt
    15841 acgcggcaat cagtgaaccg acttccgcat catcttccac aaatacgata gtgttcataa
    15901 cattcacggt agatataaaa acgtcacaat acaccgcgct gtttttactt actatcaatc
    15961 gtttctaaac aagttttcat caggtattct actgatgact tgttatggaa gtgttaaggt
    16021 aaaatcaaag ccgcgctcgg tgtgctggta gtgctgttga ttggtgtttt agcaaaaacg
    16081 aaaaattatt atatcgccgg gctgattcca cttttcccga cctttgcgct tatcgcgcat
    16141 tatattgttg ccagcgaacg cggcattgaa gccttacgcg caaccatcat ttttagtatg
    16201 tggtcgatta ttccctattt tgtttacctg gtgtcgctgt ggtatttcac cggaatgatg
    16261 cgcttgcccg ccgcttttgt tggttctgtt gcgtgttggg ggataagcgc atgggtgttg
    16321 attatatgct ggataaagct gcattaacgc agatgacgac cgccatcaag tgggaaactg
    16381 cgtccggtga caaagcaact ggtaagtaag taatcgacca ggtcgatcac ttctttctcg
    16441 ccaggcgcgg ttttcatcag tgatttattc agcgcctgtt gtcgatattc ggcatcatca
    16501 tgttcattaa acaggatcag cgatggagca atagaattca ctttcacttc cggtgccagc
    16561 ttgcgggcaa acgagcgggt catattatcc agtgccgctt tgcttgcagc atacgcaata
    16621 tgcttgtcgc taccgcgctc caccacataa tcggtaaagt gaatgatatc gctggcggcg
    16681 tgtccgtgcc cacgcagtaa tctttccagc gcatggttga gcaggtatgg ggtattaacg
    16741 tggatctgca tcatgcaagc cagtacgtcg gccagtggcg cacccagttt ttccgccatc
    16801 cacgcactgg cgttatgcaa aatagcacgc agaccatggg tgctttttag tacttcatcg
    16861 gcaaacgcca tcacaccgtc gttggtcgaa aaatcagcct gaatacactg cgcacctgcc
    16921 ttaatcagtc catcaatggc tggatagtgc gtccgatagc tgacaatcac cggttgcttt
    16981 tgattaatga aatgccaggc gagggcgagg ccgatgcgac gacctccgcc agtaattaat
    17041 attggcaagg gctgggcttt acccatcgtt atctcctttg ctatccaacg atgggatgaa
    17101 cgttatccca ccagcatcca ggtcgccgga actgaggcaa ttaacagcat gccaatcagc
    17161 accatttcct gacggttcag tacattatca tgtgtatgcg ttttgcgcgc atacaggaaa
    17221 accagcagcc ccggcgcata cagaacaacg gataacagca agtgcattgg accagaggcg
    17281 tacaataacc ataagccata aatgcaggca ccgacaccta ccgctttatg cagtggacgg
    17341 gttgcgattt tcagcaagaa cgcgccgacc aggaaatagg gcaccagaat catttctgag
    17401 gcgatggtca gcaacgtgtt gtaatcggaa ccggtcagcc agatgagtac cagacaaatt
    17461 tgcacgcaga tgttggtcaa ccacagtgag gcggatggcg cagcttgtgc gttctggcgc
    17521 gcaaaaatgc gtgggaatgc tttatgagtg gcagccagga acggaacttc tgccgccatg
    17581 atggtccagc ttaagtacgc gccgcaaacg gaaacgatca aactggcagc gatgatgatt
    17641 tcgccccatg gtcccatcat ttccaccatc agaccggcca ttgacgggtt acgaatttca
    17701 gccagttcag gacgtgccac cacacccagc gaaagcagcg ttaccagcaa gtaaacgccc
    17761 agagcggaga gaaccgccag cagtgtcgct ttgccaacat cacgtttatt acgcgcacgc
    17821 gcagaaacaa ccacggcacc ttccacacca atgaaaaccc acagggtgat cagcatggtg
    17881 tttttcacct gttcccaaac gggtacgcca agtacaagtc cggtgaagtc gagcttgaag
    17941 gtatccagtt tgaacatcat catcgccagc acaacaaaca gacccagcgg caacaatttt
    18001 gccagtgtcg ccaccaggtt aatgctggca gcggtttgca ccccgcgcag gatcaaaaag
    18061 tgaacaatcc acaataaagc cgatgcaccg acgatcgatt gccaggtatt accatcgccg
    18121 aacaggcgca attccggcgt gtccgtaaaa aagcttaacg cggaaaaaac gatcaccaga
    18181 taggagacgt tggcgatgac tgcgcacagc cagtatcccc atgcggaaca aaagccgatt
    18241 agctcgccaa acccttcgcg ggcataggta aagataccgc cgtcaagttc gggacgaatg
    18301 cgcgtgagga tcagcatggc aaaggccagc aataaaatgc cagcgccagt aataccccag
    18361 ccgatgagca gtgctgccgg gctggcaact gccgccatat tttgcggcag actgaaaaca
    18421 cccgcgccca gcattgagct taataccagc gcggtgagtg cgctcagtcc cagtttcttt
    18481 tccatcgata tccagtgatt aaaacgtgat aagaagaata aatattaggg caaaacgcat
    18541 aaaaatggag tttgccactg aagcgcggga ttttacgaag agatgtgtct gcatgcaatg
    18601 gtcgcgggga aatttgtgac taaaaatgcg aaaggcggca tcaagccgcc ttataggagt
    18661 aactgaatag ttatttatac agatctgcgc tgacggtcag gttattacca cgttcctgcc
    18721 actggcgggt gatgtggtaa tacttagcac ctttcttcgc ggcacgtttc gcaacctgat
    18781 aggagacttc ggtcatgttg ccgtagttgc cagagaattt gatgctatcg aacgggacca
    18841 tcatcgctgc ggtcgctttg ttcagttctt cgactttagt gccatccggg agcgtgacgg
    18901 tgtaacgccc gccttttgat gactgggttt caaagaaacg accgacttca gaacttggtg
    18961 atgcggtagt cgcaacacct gggatctcaa ctttcttcgc ggcttcgcca ccggcagcca
    19021 gagctgcacg tcctgcttcg gaatctgccg ggatcacatc cgggctctgg acgatacgtt
    19081 tcttagcatc ttttttatag atgaatgcag taatacgctg gttgccgccc tggttggcat
    19141 cgatttgacg aacgatgtag aaagagtagg cacctttcgc ttttgccgct ttggtgatgg
    19201 catcattgac ttctggctgg ctacgataga agccctggac ggtgaccgtg tcaaacggtt
    19261 caatcagaac agcctgatct ttcggcagtt cgacaacacc gttaattacg cgattacttg
    19321 tttcttctgc tttttcagca tcggctttat agaggtcagc gaccacacgc cagttaccgc
    19381 tgttaccaaa atcagaagtg tcgacaacat aaaaagaggc ggcaccttct ttatctgcgc
    19441 gacgagaaac ggctttcacc gcttcgccaa tagcattaaa acgaccggta accactacac
    19501 ggtcaaaagg tttaaccgct gccgcttgct ccggtgtcag ttctgttgct gcgttaacgg
    19561 agaatgccgt agcagaaagc agtgccgacg ccaggagggt gttcttaagc ttcataaaat
    19621 aatccttcgc cttgcgcaaa ccaggtactg gtattgttat taacgagaaa cgtggctgat
    19681 tattgcattt aaacggtgta actgtctgcg tcatttttca tatcacattc cttaagccaa
    19741 ttttaatcct gctcaaatga ccgtctatgc ttaaaaaaca gccgtatcag catcattact
    19801 actgaagcaa ctgaattgta taagttaatt taatgttaag tagtgaatcg tgccggggcg
    19861 atgtctcgtt ttacccgacc gtcgaagaca attatcagtc tttatccggc gttctaaggt
    19921 gtttatccca ctatcacggc tgaatcgtta atattttgcg agttcacgcc gaaatactga
    19981 tttttggcgc tagatcacag gtataatttt cagtacgtta tagggcgttt gttactaatt
    20041 tattttaacg gagtaacatt tagctcgtac atgagcggct tgtgtggctc ctgacacagg
    20101 caaaccatca tcaataaaac cgatggaagg gaatatcatg cgaattggca taccaagaga
    20161 acggttaacc aatgaaaccc gtgttgcagc aacgccaaaa acagtggaac agctgctgaa
    20221 actgggtttt accgtcgcgg tagagagcgg cgcgggtcaa ctggcaagtt ttgacgataa
    20281 agcgtttgtg caagcgggcg ctgaaattgt agaagggaat agcgtctggc agtcagagat
    20341 cattctgaag gtcaatgcgc cgttagatga tgaaattgcg ttactgaatc ctgggacaac
    20401 gctggtgagt tttatctggc ctgcgcagaa tccggaatta atgcaaaaac ttgcggaacg
    20461 taacgtgacc gtgatggcga tggattctgt gccgcgtatc tcacgcgcac aatcgctgga
    20521 cgcactaagc tcgatggcga acatcgccgg ttatcgcgcc attgttgaag cggcacatga
    20581 atttgggcgc ttctttaccg ggcaaattac tgcggccggg aaagtgccac cggcaaaagt
    20641 gatggtgatt ggtgcgggtg ttgcaggtct ggccgccatt ggcgcagcaa acagtctcgg
    20701 cgcgattgtg cgtgcattcg acacccgtcc ggaagtgaaa gaacaagttc aaagtatggg
    20761 cgcggaattc ctcgagctgg attttaaaga ggaagcgggc agcggcgatg gctatgccaa
    20821 agtgatgtcg gacgcgttca ttaaagcaga aatggaactc tttgccgccc aggcaaaaga
    20881 ggtcgatatc attgtcacca ccgcgcttat tccaggcaaa ccagcgccga agctaattac
    20941 ccgtgaaatg gttgactcca tgaagacggg cagtgtgatt gtcgacctgg cagcccaaaa
    21001 cggtggcaac tgtgaataca ccgtgccggg tgaaatcttc actacggaaa atggtgtcaa
    21061 agtgattggt tataccgatc ttccgggccg tctgccgacg caatcctcgc agctttacgg
    21121 tactaacctc gttaatctgc tgaaactgtt gtgcaaagag aaagacggca atatcactgt
    21181 tgattttgat gatgtggtga ttcgccgcgt gaccgtgatc cgtgcgggcg aaattacctg
    21241 gccggcaccg ccgattcagg tatcagctca gccgcaggcg gcacaaaaag cggcaccgga
    21301 agtgaaaact gaggaaaaat gtgcctgctc accgtggcgt aaatacgcgt tgatggcgct
    21361 ggcaatcatc cttttcggtt ggctggcaag cgttgcgccg aaagagtttc ttgggcactt
    21421 caccgttttc gcgctggcct gcgttgtcgg ttattacgtg gtgtggaatg tatcgcacgc
    21481 gctgcataca ccgttgatgt cggtcaccaa cgcgatttca gggattattg ttgtcggagc
    21541 actgttgcag attggccagg gcggctgggt tagcttcctt agttttatcg cggtgcttat
    21601 agccagcatt aatattttcg gtggcttcac cgtgactcag cgcatgctga aaatgttccg
    21661 caaaaattaa ggggtaacat atgtctggag gattagttac agctgcatac attgttgccg
    21721 cgatcctgtt tatcttcagt ctggccggtc tttcgaaaca tgaaacgtct cgccagggta
    21781 acaacttcgg tatcgcaggg atggcgattg cgttaatcgc aaccattttt ggaccggata
    21841 cgggtaatgt tggctggatc ttgctggcga tggtcattgg tggggcaatt ggtatccgtc
    21901 tggcgaagaa agttgaaatg accgaaatgc cagaactggt ggcgatcctg cacagcttcg
    21961 tgggtctggc ggcagtgctg gttggcttta acagctatct gcatcatgac gcgggaatgg
    22021 caccgattct ggtcaatatt cacctgacgg aagtgttcct cggtatcttc atcggggcgg
    22081 taacgttcac gggttcggtg gtggcgttcg gcaaactgtg tggcaagatt tcgtctaaac
    22141 cgttgatgct gccaaaccgt cacaaaatga acctggcggc tctggtcgtt tccttcctgc
    22201 tgctgattgt atttgttcgc acggacagcg tcggcctgca agtgctggca ttgctaataa
    22261 tgaccgccat tgcgctggta ttcggctggc atttagtcgc ctccatcggt ggtgcagata
    22321 tgccagtggt ggtgtcgatg ctgaactcgt actccggctg ggcggctgcg gctgcgggct
    22381 ttatgctcag caacgacctg ctgattgtga ccggtgcgct ggtcggttct tcgggggcta
    22441 tcctttctta cattatgtgt aaggcgatga accgttcctt tatcagcgtt attgcgggtg
    22501 gtttcggcac cgacggctct tctactggcg atgatcagga agtgggcgag caccgcgaaa
    22561 tcaccgcaga agagacagcg gaactgctga aaaactccca ttcagtgatc attactccgg
    22621 ggtacggcat ggcagtcgcg caggcgcaat atcctgtcgc tgaaattacc gagaaactgc
    22681 gcgctcgtgg tatcaacgtg cgtttcggta tccacccggt tgcggggcgt ttgcctggac
    22741 atatgaacgt attgctggct gaagcaaaag taccgtatga catcgtgctg gaaatggacg
    22801 agatcaacga tgactttgct gataccgata ccgtactggt gattggtgct aacgatacgg
    22861 ttaacccggc ggcgcaggat gatccgaaga gtcctattgc tggtatgcct gtgctggaag
    22921 tgtggaaagc gcaggacgtg attgtcttta aacgttcgat gaacactggc tatgctggtg
    22981 tgcaaaaccc gctgttcttc aaggaaaaca cccacatgct gtttggtgac gccaaagcca
    23041 gcgtggatgc aatcctgaaa gctctgtaac cctgacggcc tctgcggagg ccgtcactct
    23101 ttattgagat cgcttaacag aacggcgatg ctttgacctc ccgctgtttg ttcaagcgca
    23161 attttgacaa taattgttaa cggcacggaa agcagcatac ccaccggtcc taacaaccat
    23221 ccccaaaaaa tcaacgacaa aaataccacc aatgtggaaa gccccagccc acgtcccatg
    23281 atgcgcggct ccagaatatt gccgaagacc agattaatca gcagatatcc cgccagcacc
    23341 agcaacgctt cgtagaagcc attaaacacc agtacctgag cgataggggg gattgccgcg
    23401 agaactgaac caatattagg gatgtaatta agcgcaaagg ccagcaatcc ccagacaaaa
    23461 gcgaagcgaa catcgagtgc ggcgagcatc gcccaggcga ccaggccggt gatgatgctg
    23521 atggctgttt tcagcaccag ataatgagaa acactgtcaa ttgcacgttg aatcgccgcc
    23581 atcccttcaa ccggacgcgc catcatttgc tgaaattttc cgggcaattg tggcacttcg
    23641 agcagcataa acagcaccgt cagcagcaat aaaaatattg ataacatggc attagataac
    23701 tgcgtcaata agttggtgag caacgtcatc gctgcgttcg gatcaatgta atgcgccagc
    23761 tggtcaactg agacgtcaat ccctacgcgt tgcaacaacg gctcaagagc ttgcagcggc
    23821 gtcataatag agttgcgata ttgcggtaac gtccgcgtca actcgttgag cgcggaaccc
    23881 agataagcta atagcaacac catcgccatc acgatgatgg tcatcaaaat cgacaccgcc
    23941 agtacacgcg gcactcgcca gcggaccatg tgttgcacca gcgggttaag aataacagca
    24001 ttttatcatt tagtgttttt tgctgtttta taatgtttca ggctgtgcat tatgtttttg
    24061 attatcaaca gatttgtgat tctccttgtt ccagcttgtt tcacgttgta gccctttgtg
    24121 caacgatcgg tgtagtcact ggtgtagtca ctttttcgta aagagggggt aaaacgtgag
    24181 cagagcaatg aacaaactga gcgatacctt gctaaggaaa cttcatggta agccagcgga
    24241 gaaaaacacg ttttatagtg atggcggaaa cctgagcgtg aaatatttaa catcagggaa
    24301 attgacctgg tatttcatat acagggccgg aacgggaagg gagacacgac ccgaacgcat
    24361 taagctggga agttatcctg atctgagcct gaaatcagcc agggaaaaag ccgcccagtg
    24421 tcgcgcatgg ctggcagagg ggaaaaatcc acgtcatgag cttaattaca ccgtacagga
    24481 agcgttaaag ccggtaacgg ttggcgatgc gctcacctac tggcttgagt attacgtaaa
    24541 ggaaaaccgc gtggattata tcgccctgaa aaggcgactt aataatcacg taatacagca
    24601 gattggtgct atgccgctgg ataaatgcga gctacggcac tggctggcct gttttgacca
    24661 ggtggcaaag cgaacgcctg ttactgccgg attcgtgcta caggcgtgca aacaggcgct
    24721 taagttctgc cgtaggcggc gctatgcaat cagcaacgtt ctggacgatc tgaacgtggc
    24781 ggacgttggg aaaaaaccgg atataagcga acgtgtctta agtaacaaag aacttggcga
    24841 attattgcag gcactggaca aaaaaatatt ctccccttac tacgtcgcgt taatccgcct
    24901 cctgattgtc ttcggagcca gaacggtaga actgaggtta tcggagatcg gggagtggga
    24961 ttttacggaa atgctatgga ccgttccgaa ggagcacagc aaaacgaagg tcgcaatatt
    25021 ccggcccata ccggaagcga tcctgccgtt cgtcacgcag ctggtggagc agaacaggca
    25081 cacgggctta ttgctggggg aagtgaaaca ggaggccagc gtatcgcagt atggaagatt
    25141 agcgcacagg aggcttaatc atcctcactg gtcactgcat gacatccggc gcacctttac
    25201 aaccatgctg aacgatttag gcgttgatcc gcatgtcgtg gagcagctta caggccacca
    25261 gatgccagga atgcagcgag tttataatca ttcccgttat ctggatgcta aacgcaatgc
    25321 gctggatatg tggacggagc ggttagggat actggcggga acacatgaaa acgtaaccac
    25381 gctaccagta gccagaagaa aataattttt ttcgtgtttt ttcagtatgc gcatactgta
    25441 tatgcgaaca gatacaacgt gcaacaatga gcaacaatgt ggaacaacca cgaacaagag
    25501 gcgaaaaagc atacagattt ataagtggct gatttttaat gtgtcactgg ttttttatac
    25561 actagcgaat cactctttaa agagcgagaa aaaaggcatg aaacaatatt ttatgccttt
    25621 gtattaacat gaatttaaaa gattttattc ttactgtttc attttctgta aatcatacat
    25681 cctcaatatt tgccattcat cactctagcg ggaacaatac gacacaataa gacacctgat
    25741 gactctttaa gaaacgaaag ggggggcaat agtgttaagc actgatcggt ttatacgtga
    25801 aaaagaatgc gaaaaactaa ccggccttag ccgtacgtgt cgctaccgcc tggaaaaggc
    25861 cggacaattc ccatcacgtc gtaaacttgg cggtcgttcc gttggctggt ctttatccga
    25921 ggttctggcc tggaaggata gctgcaaggc agttcattaa tcacgctggc ggcacgcagc
    25981 cgccacacat caatcatctg aacacagagc tataaccatg aagattgaat atacgccaga
    26041 acgtgggcgg ggattcgttc gccctggtga aactgaaaaa caacaaaatt ggggtttttc
    26101 aggtataaaa aaagcggccc cgaaatggag ccgcctttct gaacagataa cccgctgcgc
    26161 cgtttgtgtg tgtgatccca aacataagca cggggatgat agccgctatc aggctggtgg
    26221 gcaatgcaat cagtctggtt cagttcgttg ccatacctgc aatgagcgct tttccctgta
    26281 ctctttaagg aattgctcaa gggcaaaagc acatggcgcg aatctttctg attcatgctc
    26341 tatctttctg cgccgtcttt tccgtgccgg tgataatgtt ttggtcaatt ctttatcggt
    26401 cattgtgttg tcctgcatag caatgcgccg taatacctta caccacggcg ctgatggtga
    26461 ttactctggt tctttggcct tgcgccgctg gcgatattca acttctcgtt ttaatgccgc
    26521 agtaacaaat tgccccgtac tttcgccagg catttttacc gcctcaacat tgttcataac
    26581 ttcatgcgga acccttgccg caacggtttg tgattttgcg tttactgctt ttgtcgccat
    26641 gtggtgtacc cctcataaaa aacaaatgca gtatgcagga aaaaaaataa gtgttcaaca
    26701 cttgacgtgt ttaacacctg ggtttaaatt ggtgttcaac accttgttgg cgcaaggtgc
    26761 agaaacgacg aaacccggca gtgcgctaac actaaccggg cttctaacca ccaacgatag
    26821 cgaaagtatc gaggtagcta tgagaaatca taccatacac ccgcaagggc gggactcgca
    26881 caacctgaat aaatacatct ggcgttttat cgccctgagc acggcacaac cgcgcgtgat
    26941 taccattgag gccaccagcg aacaggaagc acgccagcaa tctccggctg gctgcgtgat
    27001 ggtatttgct gcccgtattc gtcagggggt aggcttatga gccaggaaat cacactacaa
    27061 caggcagcag aacgcgccca ccaaatcgaa gttatttgcg cactggcaga ggattaccct
    27121 ggcatgatga ccgacagcga atcaggggca atcatcggct tacttaaacg ccttagcggt
    27181 gaggtctgcg tattcctgag cgatgaacag gaaagaagaa cgcttatttc taacgaaaaa
    27241 aaatgcggag gggtacatgt gcaataaaac cacaccggac gcagccgccg ccgcgctcac
    27301 tacgctgatg cacgcgctta ttgatatttc tgttattgct gacagggcgc ataaacacgc
    27361 agccagagaa tcagaatgca tcttccatta tttaacattt gtgcagctaa aagccgatca
    27421 ggcactggat aaggccggaa aaattatcat ggctgatgtg caggaggtgc accatgcata
    27481 acctgtcaat ttctgacctt aacagcattc agtttgacga gaaatttacc gggcagttgc
    27541 tggtcaatgt ggagaacggg cgcatagtgc gtaattatca cctgccggat ggtgcaattg
    27601 ccggaagcgt tgaagcattg ctggaactgg cggaacgtgc gcgactgatt aagccgtcaa
    27661 cgagccatca cgatgatgat ctgcatttta ccggatgtat ggtgagtcac tacgaaaacg
    27721 gcgtcgaggt atcctgcgaa cggctgcgtg atgattgctg tttcggcaca ctgccggaat
    27781 ttatcgagtt gttgaccagt tgcggttatc aggtcattca gggggtaaac atgcgtgatg
    27841 atcgttttaa ttccctgaaa caggaatttt ccggcgttcc tgatgatgcg gctgatgcgc
    27901 tttcgtcaat gccagagctt attagagcgg cttttttctt actttccacg agagaatata
    27961 aatcaacggg gcttgatgta ctgaatatcg tcgccgatta tgctgatttc gtgacagagg
    28021 ttattttaag aaaaacgacg gacggggatt aatatgcgtg atatttacct tgaaacaata
    28081 gaccgcgcat ttcttgcact ttctcacagt gaaaacatgc tggaaatatt acgcatatgg
    28141 cttgaaacac ttggcgacaa tgaacgcgac aaacaaaaat caagaattgc cacggcatta
    28201 ataacgcttc ttgagcctgt aataatggaa ctgcaagaaa tagatctatt gcacgacaga
    28261 tataaagaac agcacaccgg agaataaaaa taatgaaact taaatattct ggcttaactg
    28321 ccagtggcaa cactcaccct aaatttacgc gcggcgatat ttaccgcgac cagtacggcg
    28381 gcaccgtaac gattaaaggt gtagaagagc gacgcattac ctaccgccgt gaaggctacg
    28441 aatatgactg cgtgatgcct gtttatcagt tccggcgtga tttttctctg gtacaggcag
    28501 cgccccgcag taaaccaacc agcagggaga aagcacgcgc caatattcag gaaataaaaa
    28561 agatgcttaa cgtattcagg ggcaagaaat gaaaagcgca ccgaacttaa aaaaacagcc
    28621 ttacgacaag atgaccgaag tcatcatttt tgcgggtagt gatgcgtggg cacatgc
//
LOCUS       DAJVTY010000034        28237 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVTY010000034 DAJVTY010000000
VERSION     DAJVTY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28237
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.034"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="OQQ66_001368"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="OQQ66_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1904353.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="OQQ66_001369"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="OQQ66_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1904354.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="OQQ66_001370"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="OQQ66_001370"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1904355.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="OQQ66_001371"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="OQQ66_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1904356.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3240..4634
                     /gene="ychO"
                     /locus_tag="OQQ66_001372"
     CDS             3240..4634
                     /gene="ychO"
                     /locus_tag="OQQ66_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1904357.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4635..5285)
                     /gene="narL"
                     /locus_tag="OQQ66_001373"
     CDS             complement(4635..5285)
                     /gene="narL"
                     /locus_tag="OQQ66_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1904358.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5278..7074)
                     /gene="narX"
                     /locus_tag="OQQ66_001374"
     CDS             complement(5278..7074)
                     /gene="narX"
                     /locus_tag="OQQ66_001374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1904359.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7413..8804
                     /gene="narK"
                     /locus_tag="OQQ66_001375"
     CDS             7413..8804
                     /gene="narK"
                     /locus_tag="OQQ66_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1904360.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9320..13042
                     /locus_tag="OQQ66_001376"
     CDS             9320..13042
                     /locus_tag="OQQ66_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1904361.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13060..14598
                     /gene="narH"
                     /locus_tag="OQQ66_001377"
     CDS             13060..14598
                     /gene="narH"
                     /locus_tag="OQQ66_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1904362.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14595..15305
                     /gene="narJ"
                     /locus_tag="OQQ66_001378"
     CDS             14595..15305
                     /gene="narJ"
                     /locus_tag="OQQ66_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1904363.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15305..15982
                     /gene="narI"
                     /locus_tag="OQQ66_001379"
     CDS             15305..15982
                     /gene="narI"
                     /locus_tag="OQQ66_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1904364.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16165..16296)
                     /locus_tag="OQQ66_001380"
     ncRNA           complement(16165..16296)
                     /ncRNA_class="other"
                     /locus_tag="OQQ66_001380"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16344..16428)
                     /locus_tag="OQQ66_001381"
     tRNA            complement(16344..16428)
                     /locus_tag="OQQ66_001381"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16392..16394),aa:Tyr,seq:gta)
     gene            complement(16463..16547)
                     /locus_tag="OQQ66_001382"
     tRNA            complement(16463..16547)
                     /locus_tag="OQQ66_001382"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16511..16513),aa:Tyr,seq:gta)
     gene            complement(16707..17549)
                     /gene="purU"
                     /locus_tag="OQQ66_001383"
     CDS             complement(16707..17549)
                     /gene="purU"
                     /locus_tag="OQQ66_001383"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1904365.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17599..18057)
                     /locus_tag="OQQ66_001384"
     CDS             complement(17599..18057)
                     /locus_tag="OQQ66_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1904366.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18170..19075
                     /gene="rssA"
                     /locus_tag="OQQ66_001385"
     CDS             18170..19075
                     /gene="rssA"
                     /locus_tag="OQQ66_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1904367.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19167..20180
                     /gene="rssB"
                     /locus_tag="OQQ66_001386"
     CDS             19167..20180
                     /gene="rssB"
                     /locus_tag="OQQ66_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1904368.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20382..21290
                     /gene="galU"
                     /locus_tag="OQQ66_001387"
     CDS             20382..21290
                     /gene="galU"
                     /locus_tag="OQQ66_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1904369.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21434..21847)
                     /gene="hns"
                     /locus_tag="OQQ66_001388"
     CDS             complement(21434..21847)
                     /gene="hns"
                     /locus_tag="OQQ66_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1904370.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKARRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22452..23069
                     /gene="tdk"
                     /locus_tag="OQQ66_001389"
     CDS             22452..23069
                     /gene="tdk"
                     /locus_tag="OQQ66_001389"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1904371.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23351..24247)
                     /locus_tag="OQQ66_001390"
                     /pseudo
     CDS             complement(23351..24247)
                     /locus_tag="OQQ66_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24371..27046)
                     /gene="adhE"
                     /locus_tag="OQQ66_001391"
                     /gene_synonym="adhC"
     CDS             complement(24371..27046)
                     /gene="adhE"
                     /locus_tag="OQQ66_001391"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1904372.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27524..28171
                     /gene="ychE"
                     /locus_tag="OQQ66_001392"
     CDS             27524..28171
                     /gene="ychE"
                     /locus_tag="OQQ66_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1904373.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         6932 a         6787 c         7363 g         7155 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagtgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcgtcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaagcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aaatctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgagcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc gcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catgaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagtcg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt tttcattatt accggaagtt accgacgttt
     3241 tgagccgttt cgttcctcgc attatcccgt tttatttact cttgcttgta gcaggcggta
     3301 cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
     3361 atggtctgcc ggatttaggc atggctcccg aaaatcatga tggggaaaaa cactttgctg
     3421 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
     3481 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
     3541 acgtagagtc ctggctatca ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg
     3601 aaggacattt caccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
     3661 atttcacctg gagccagctt ggtcttactc agcaggataa tgggttggtg agcaatgtgg
     3721 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
     3781 acttgctgga cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
     3841 tgcgattatt ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc
     3901 aggaacaacg gatggcgcgc gggtacgacc tgacagctcg gatgcgcatg ccgttctatc
     3961 aacacctcaa taccagtgtc agcctagaac agtattttgg tgatcgtgtt gatttgttta
     4021 actctggtac gggttatcac aatcccgtcg cgttgagtct gggattaaat tacacccctg
     4081 tgccattagt cactgtgacg gcccagcata aacagggtga aagtggcgag aatcaaaata
     4141 acctcgggct gaatcttaat taccgctttg gtgtaccgct caaaaaacaa ctttctgcgg
     4201 gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta tgacaatccg cagcgaaata
     4261 atctaccgac tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc
     4321 cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg
     4381 gtattcggca actgatttgg cagggcgata cgcagatatt aagtttgacg ccgggcgcac
     4441 aagccaacag cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg
     4501 caagcaatca ctggcgattg tcggtggtgg tggaatataa ccaggggcag cgtgtctcct
     4561 ccaatgagat cacgctaacg cttgtcgaac cgttcgatgc attgtcaaac gacgaactgc
     4621 gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga
     4681 gacttgagct tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg
     4741 atatccaggc ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata
     4801 tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc
     4861 aagctggcgg ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc
     4921 tgatgcaatg ctttcagcag atcttccggt tccatatctt ttaacagata accatccgcg
     4981 ccgcgtttca gtgcggtgac cacatcttct tcatggttag agacgctgaa taccacaatg
     5041 cgccctgaga gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc
     5101 atattgagat ctaacaggat cagatcggga tcaagagact ccgccagttc aataccctgt
     5161 tcgccattac tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt
     5221 acgccagttc gcagcatcgg gtgatcgtcg atcagcagga tagtagccgg ttcctgatta
     5281 ctcatgggta tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac
     5341 ttcggtgcca cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg
     5401 gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccgca
     5461 gccgttatct tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac
     5521 ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa
     5581 gtggattgcc tgatgtgaag gcaccagacg aggcggcaat tgataatcca gcttcaccgg
     5641 gaagccaaat ttggcgctgt actcttcgca actcgcctcc agcgccggac gtaatccagg
     5701 ctcggtgagc tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt
     5761 cagttcgtta cggatctgac ttaacagttc gcggctgctt gctggcagcg catcgccctg
     5821 catctgtaaa caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg
     5881 cagttcgcgc gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg
     5941 atggcgatcc agcgccagcg tggcggtgag ttgttcaacc agggtatcca ccagttgttg
     6001 ttgatcatgg ctaagatgac gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt
     6061 atgagagtca gccagccgcc acttcagggt tgtgccgcga tcaccaacgg gtaatacgcc
     6121 gcgcgggcag agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc
     6181 ctgatgattc tcttcatcat cagtgtcata cacccgcagt tcgatatcac gtagcagggt
     6241 taaattctgt aagccgttga gtacaggtga caggcgttca cacagcgggg cgcgggaatg
     6301 caaacggcgg ttagcctgcc ataaaaaaga gaggatctga tttttatgct ccagcccggc
     6361 ggttttctcc tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat
     6421 attgttcaat gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt tcgcgcgttg
     6481 ggtaaaatcg cgatgactga cggcactcgc cattgccagc agttgccgcc acggttgtag
     6541 cagtcgcgcc cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat
     6601 tacccgatgg accagtacca ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga
     6661 taccagctga tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttcgcggtt
     6721 ttgtgcacgc atcagtgcag ggatcagttc attacgccag taatcttgta aaccctgtaa
     6781 ttgcgccagt tgttcgtctc gttctgctgc tcgagttaac tcggcgctaa atgccgtttg
     6841 ttccatctct ttaattaagg gcttgtcttt ctcacttaat ggcactgccg ccaacagacg
     6901 gtaactttgc atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc
     6961 ttgcaccagc cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac
     7021 aataagcgca acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgtaaac
     7081 ctcttccttc aggctttaaa tgagcaataa ccttaatgaa tgtgacgata cattctggaa
     7141 tggcagtatt ctcggctatt ggctgaagta tacccatacc cggaaagagt tactccttat
     7201 ttgccgtgtg gttagtcgct ttacatcggt aagggtaggg attttacagc accgtgaaaa
     7261 atctcataat ttttatgaaa tcactgtact cactatgggt attgataaat atcaatgata
     7321 gataaagtta tcttatcgtt tgatttacat caaattgcct ttagctacag acactaaggt
     7381 ggcagacatc gaaacgagta tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg
     7441 ctactggagc tgtcattaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg
     7501 gtcaacgtat tgccagccgt aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt
     7561 gcgtatggat gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta
     7621 cgaccgatca gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg
     7681 ttccatactc ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg
     7741 gtattctgat tattccttgc gtctggctgg gttttgccgt gcaggatacc tccacgcctt
     7801 atagcgtctt catcatcatc tctctgctat gcggctttgc tggcgcgaac ttcgcatcca
     7861 gtatggcaaa catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga
     7921 atggtggtct gggaaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat
     7981 cactgtcgat tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc
     8041 tgtatctggc gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg
     8101 cgtggtttgg catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg
     8161 tactcaaacg gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct
     8221 tcatcggctt ctccgcgggc tttgcaatgc tgtcaaaaac gcagttcccg gatgttcaga
     8281 ttctgcaata cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg
     8341 cattatctga ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga
     8401 ttttcagcgg cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg
     8461 cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt
     8521 tccagatgat ttcagtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg
     8581 gttctgacga acgtgcgatg cgtgaagcgg caaccgacac ggcggcggcg ctgggtttca
     8641 tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg
     8701 cattaacggg ttcgccagtc ggcgcaatga aggtattttt gattttctat atcgcctgcg
     8761 tagtgattac ctgggcggta tatggtcggc attctaaaaa ataaaccgtt actcgtcata
     8821 ctccgggtta catgtgctgc ggctgcgttc attcacccca gtcacttact ttagtaagct
     8881 cctgggattc attcacttgc cgccttcctg taaaccgaat tatatagagt aaaatatttg
     8941 attatccttt gcgcggcatg atgtcgcgct ttttttaagc gttatttagt tacaacatac
     9001 taatgttata tggtttattt cgccggattt cattaagagc cattaatatg ttccccatgg
     9061 ggaatactcc ttaataccca tctgcataaa aatcttaata gtttaaataa ctacaggtat
     9121 aaaacgtctt aatttacagt ctgttatgtg gtggctgttg attatcctaa aggggtatct
     9181 taggaattta ctttattttt catccccatc actcttgatc gttatcaatt cccacgctgt
     9241 ttcagagcgt taccttgccc ttaaacatta gcaatgtcga tttatcagag ggccgacagg
     9301 ctcccacagg agaaaaccga tgagtaaatt cctggaccgg tttcgctact tcaaacagaa
     9361 gggtgaaacc tttgccgatg ggcatggcca gcttctcaat accaaccgtg actgggagga
     9421 tggatatcgc cagcgttggc agcatgacaa aatcgtccgc tctacccacg gggtaaactg
     9481 caccggctcc tgcagctgga aaatctacgt caaaaacggt ctggtcacct gggaaaccca
     9541 gcagactgac tatccgcgta cccgtccgga tctgccaaac catgaacctc gcggctgccc
     9601 gcgcggtgcc agctactcct ggtatcttta cagtgccaac cgcctgaaat acccgatgat
     9661 gcgcaaacgc ctgatgaaaa tgtggcgtga agcgaaggcg ctgcatagcg atccggttga
     9721 ggcatgggct tctatcattg aagacgccga taaagcgaaa agctttaagc aggcgcgtgg
     9781 acgcggtgga tttgttcgtt cttcctggca ggaggtgaac gaactgatcg ccgcatctaa
     9841 cgtttacacc atcaaaaact acggcccgga ccgtgttgct ggtttctcgc caattccggc
     9901 aatgtcgatg gtttcttacg catcgggtgc acgctatctc tcgctgattg gcggtacttg
     9961 cttaagcttc tacgactggt actgcgacct gcctcctgcg tctccgcaaa cctggggcga
    10021 gcaaactgac gtaccggaat ctgctgactg gtataactcc agctacatca tcgcctgggg
    10081 ttcaaacgtg ccgcagacgc gtaccccgga tgctcacttc tttactgaag tgcgttacaa
    10141 aggcaccaaa actgttgccg tcacaccaga ctacgctgaa atcgccaaac tgtgcgatct
    10201 gtggctggca ccgaaacagg gcaccgatgc ggcaatggcg ctggcgatgg gccacgtaat
    10261 gctgcgtgaa ttccacctcg acaacccaag ccagtatttc accgactatg tgcgtcgcta
    10321 caccgacatg ccgatgctgg tgatgctgga agaacgcgac ggttactacg ctgcaggtcg
    10381 tatgctgcgc gctgctgatc tggttgatgc gctgggccag gaaaacaatc cggaatggaa
    10441 aactgtcgcc tttaatacca atggcgaaat ggttgcgccg aacggttcta ttggcttccg
    10501 ctggggcgag aagggcaaat ggaatcttga gcagcgcgac ggcaaaactg gcgaagaaac
    10561 cgagctgcaa ctgagcctgc tgggtagcca agatgagatc gctgaggtag gcttcccgta
    10621 ctttggtggc gacggcacgg aacacttcaa caaagtggaa ctggaaaacg tgctgctgca
    10681 caaactgccg gtgaaacgcc tgcaactggc tgatggcagc accgccctgg tgaccaccgt
    10741 ttatgatctg acgctggcaa actacggtct ggaacgtggc ctgaacgacg ttaactgtgc
    10801 aaccagctat gacgatgtga aagcttatac cccggcctgg gccgagcaga ttaccggcgt
    10861 ttctcgcagc cagattattc gcatcgcccg tgaatttgcc gataacgctg ataaaacgca
    10921 cggtcgttcg atgattatcg tcggtgcggg gctgaaccac tggtatcacc tcgatatgaa
    10981 ctatcgtggt ctgatcaaca tgctgatttt ctgcggctgt gtcggtcaga gcgggggcgg
    11041 ctgggcgcac tatgtaggtc aggaaaaact gcgtccgcaa accggctggc agccgctggc
    11101 gtttgccctt gactggcagc gtccggcgcg tcacatgaac agcacttctt atttctataa
    11161 ccactccagc cagtggcgtt atgaaaccgt cacggcggaa gagttgctgt caccgatggc
    11221 ggacaaatcc cgctataccg gacacttgat cgactttaac gtccgtgcgg aacgcatggg
    11281 ctggctgccg tctgcaccgc agttaggcac taacccgctg actatcgctg gcgaagcgga
    11341 aaaagccggg atgaatccgg tggactatac ggtgaaatcc ctgaaagagg gttccatccg
    11401 ttttgcggca gaacaaccag aaaacggtaa aaaccacccg cgcaacctgt tcatctggcg
    11461 ttctaacctg ctcggttctt ccggtaaagg tcatgagttt atgctcaagt acctgctggg
    11521 gacggagcac ggtatccagg gtaaagatct ggggcaacag ggcggcgtga agccggaaga
    11581 agtggactgg caggacaatg gtctggaagg caagctggat ctggtggtta cgctggactt
    11641 ccgtctgtcg agcacctgtc tctattccga catcattttg ccgacggcga cctggtacga
    11701 aaaagacgac atgaatactt cggatatgca tccgtttatt cacccgctgt ctgcggcggt
    11761 cgatccggcc tgggaagcga aaagcgactg ggaaatctac aaagccatcg cgaagaaatt
    11821 ctccgaagtg tgcgtcggcc atctgggtaa agaaaccgac atcgtcacgc tgcctatcca
    11881 gcatgactct gccgctgaac tggcgcagcc gctggatgtg aaagactgga aaaaaggcga
    11941 gtgcgacctg atcccaggta aaaccgcgcc acacattatg gtcgtagagc gcgcttatcc
    12001 ggcgacttac gaacgcttta cctctatcgg cccgctgatg gagaaaatcg gtaatggcgg
    12061 taaagggatt gcctggaaca cccagagcga gatggatctg ctgcgtaagc tcaactacac
    12121 caaagcggaa ggtccggcga aaggccagcc gatgctgaac accgcaattg atgcggcaga
    12181 gatgatcctg acactggcac cggaaaccaa cggtcaggta gccgtgaaag cctgggctgc
    12241 cctgagcgaa tttaccggtc gtgaccatac gcatctggcg ctgaataaag aagacgagaa
    12301 gatccgcttc cgcgatattc aggcacagcc gcgcaaaatt atctccagcc cgacctggtc
    12361 tggtctggaa gatgaacacg tttcttacaa cgccggttac accaacgttc acgagctgat
    12421 cccatggcgt acgctctctg gtcgtcagca actgtatcag gatcaccagt ggatgcgtga
    12481 tttcggtgaa agcctgctgg tttatcgtcc gccgatcgac acccgttcgg tgaaagaagt
    12541 gataggccag aaatccaacg gcaacccgga aaaagcgctc aacttcctga cgccgcacca
    12601 gaagtggggg atccactcca cctacagcga caaccagttg atgctgacct taggtcgcgg
    12661 tggcccggtg gtctggctga gcgaagccga tgccaaagag ctgggcattg ccgataacga
    12721 ctggattgaa gtttacaaca gcaacggtgc gctgactgcc cgtgcggttg tcagccagcg
    12781 tgttccggca gggatgacca tgatgtacca cgcgcaggaa cgtatcgtta acctgccggg
    12841 ttcggaaatt actggccagc gtggggggat ccacaactcg gtcacccgta tcacgccgaa
    12901 accgacgcat atgattggtg gctatgccca tctggcatac ggctttaact actacggcac
    12961 cgtaggttct aaccgcgatg agtttgttgt agtgcgtaag atgaagaaca ttgactggtt
    13021 agatggcgaa ggcaatgact aggtacagga gagcgtaaaa tgaaaattcg ttcacaagtc
    13081 ggcatggtgc tgaatctcga taagtgcatc ggctgccaca cctgttcagt tacctgtaaa
    13141 aacgtctgga ccagccgtga aggcgtggag tacgcgtggt tcaacaacgt ggaaaccaag
    13201 ccgggtcagg gcttcccgac tgactgggaa aaccaggaaa aatacaaagg cggctggatc
    13261 cgtaaaatca acggcaaact gcagccgcgc atgggtaacc gtgccatgtt gctgggtaaa
    13321 atcttcgcta acccgcatct gccggggatc gacgattatt acgagccgtt cgattttgac
    13381 tatcagaacc tgcataccgc gccggaaggc agcaaatcgc agccgattgc ccgtccccgt
    13441 tcgctgatta ccggggaacg gatggcgaaa atcgaaaaag ggccgaactg ggaagatgac
    13501 ctgggtggtg agtttgacaa actggcgaaa gacaagaact tcgacaacat ccagaaggcg
    13561 atgtatagcc agttcgaaaa cacctttatg atgtatttgc cgcgcctgtg cgaacactgc
    13621 ctgaacccgg catgtgtggc gacctgcccg agcggtgcga tttacaagcg tgaagaagat
    13681 ggcatcgtcc tgatcgacca ggataaatgc cgtggctggc gtatgtgcat caccggatgc
    13741 ccgtacaaaa aaatctactt caactggaag agcggtaagt ctgagaagtg catcttctgc
    13801 tatccgcgta ttgaagcggg tcagccgacc gtgtgctcag aaacctgtgt cggtcgtatc
    13861 cgttatcttg gcgtgctgct gtacgatgcc gacgcgattg aacgtgcagc cagcaccgag
    13921 aacgagaaag atctttacca gcgtcagctg gaggtgttcc tcgatccgaa cgatccgaaa
    13981 gtcatcgagc aggcgattaa agacggtatt ccgctgagcg ttattgaagc cgcacagcag
    14041 tcgccggttt ataaaatggc aatggaatgg aaactggcgc tgccgctgca tccagaatat
    14101 cgcacactgc cgatggtctg gtacgtgccg cctctgtctc cgattcagtc tgcagcagac
    14161 gcgggtgagc tgggtagcaa cggcattctg ccagacgtcg aaagcttgcg tattccggta
    14221 cagtatctgg cgaatctgct gaccgccggt gataccaaac cggtactgcg cgcactgaaa
    14281 cgtatgctgg cgatgcgtca ttacaaacgt gctgaaaccg ttgacggtaa agttgatacc
    14341 cgtgcgctgg aagaggtcgg tctgaccgaa gcccaggcac aggagatgta ccgttatctg
    14401 gctattgcta actacgaaga tcgctttgtg gtgccgagta gtcatcgtga actggcacgg
    14461 gaagccttcc cggagaaaaa tggctgcggc tttacctttg gtgatggctg ccacggctca
    14521 gataccaaat tcaatctgtt caacagccgt cgtatcgatg ccatcgatgt gaccagcaaa
    14581 acggagccgc atccatgatc gaactcgtga ttgtatcgcg tctccttgaa tatccggatg
    14641 ctgccttatg gcagcatcaa caagagatgt ttgaggtgat tgctgcgtcg aaaaatctgt
    14701 cgaaagaaga tgcccatgcg ctgggcattt tcctgcgcga tttaacggcg atggatccgc
    14761 tcgatgccca ggcccagtac agcgaactgt tcgaccgtgg ccgcgccacg tcactgttgc
    14821 tatttgaaca tgttcacggt gaatcccgtg accgcggcca ggcgatggtg gacctgctgg
    14881 cgcagtacga gcagcacggc ttgcagttaa acagccgcga attaccggac catctgccgc
    14941 tgtatctgga gtacctgtcg cagctgccgc aaagcgaagc cgtggaaggt ttgaaagata
    15001 tcgcgccgat tctggcattg ctgagcgcgc gtctgcaaca gcgtgaaagc cgttatgccg
    15061 tgatgtttga tctgctgctg aaattggcca ataccgctat cgacagcgac aaagtggcgg
    15121 aaaaaattgc cgacgaagcg cgcgatgata cgccgcaggc gctggatgct gtctgggaag
    15181 aagagcaggt taaattcttt gctgacaaag gctgcggtga ttcagcaatc actgctcatc
    15241 agcgtcgctt tgccggtgcc gtcgcgccgc aatatctgaa tatcaccacc ggaggacagc
    15301 actaatgcaa ttcctgaata tgttcttctt tgatatctac ccgtacatag ccggggcggt
    15361 cttcctgatt ggtagctggc tgcgttatga ctacgggcag tacacctggc gcgcggcgtc
    15421 cagccagatg ctggatcgca aagggatgaa cctggcgtcg aacctgttcc atatcggtat
    15481 tctggggatt tttgctggtc acttcttcgg tatgctgacg ccgcactgga tgtatgaagc
    15541 ctggctgccg attgaagtga aacagaaaat ggcgatgttt gctggtggtg ccagcggcgt
    15601 gctgtgtctg attggcggcg tgctgttgct gaagcgtcgt ctgttcagcc cacgcgtgcg
    15661 tgcaaccact accggagcgg atatcctgat cctgtcgctg ctcgttatcc agtgcgcgct
    15721 gggcctgttg accattccgt tctccgctca gcatatggac ggtagcgaga tgatgaaact
    15781 ggttggctgg gcgcagtcgg tggtgacctt ccacggtggc gcttctcaac acctcgatgg
    15841 tgtggcgttt atcttccgtc tgcacctggt gctggggatg acgttattcc tgctgttccc
    15901 gttctcgcgt ctggtacaca tctggagcgt accggtggag tatctgacac gtaagtacca
    15961 gctggtgcgc gctcgtcact aaacgaattt tagttcacat agaccctgct tcggcggggt
    16021 tttttatggg cacggtgcgg ggtgagttgt cggatgcgct tcgcttatcc gacctacagg
    16081 ggaggatatt gtaggcccgg taaacgtggt gccatcgggc aaaattagct cggagtaaca
    16141 ggttttgatt attcaaggaa gcgatggtgg tgggggaagg attactcagc gctgcgcgct
    16201 tcgcccttcg ggtcgttgcc tgcggcaacg ctctctcgct ggcgctcaag tcgaaccttg
    16261 gtcgaagctt ctcatccttc cccgcttggg cagaatattt aattgcggat tcgtttgaga
    16321 gtttacggac ttttgaaaag tgatggtggt gggggaagga ttcgaacctt cgaagtcgat
    16381 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccag gggtaattca
    16441 aattttgagg taatgcttga gatggtggtg ggggaaggat tcgaaccttc gaagtcgatg
    16501 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccacg gggtaatgct
    16561 ttttactggc ctgctccctt atcgggaagc ggggcgcatc atatcaaatg acgcgccgct
    16621 gtaaagtgtt acgttgagaa aaatgaactg gttgcgtaat tttcatccgt aacggattaa
    16681 aggtaaccag ttatttttgc tggcgattaa agaataatcg tccgattacc gtaaacaaag
    16741 acgcgctgtg ccagtacttt gtatagcgca cgacttaaaa cgtttttctc gacgtcacga
    16801 cctgcgcgca tcatatcttc agctgtgtag gtatgatcga catgaataac gtcctgcatg
    16861 atgattgggc cttcgtccag attgtcattc acatagtgag cggttgcgcc aataatcttc
    16921 acaccacgtt cataggcctg gtgataagga cgtgcgccga taaacgcagg caggaatgaa
    16981 tggtgaatat tgatgatttt attcgggaag cgtgccacaa attccggcgt taatacccgc
    17041 atatacttcg ccagcaccac atagtcaggt ttataagcat caatggcgtc tgccatcttt
    17101 tgatcgtgct cgttgcggct taacccttca tggtttacca gctcaaacgg aatatcaaaa
    17161 cgctcaacca gagaacgtaa agtatcgtgg ttaccaataa ctgccgcgat ttcgacatcc
    17221 aggccgccgt aattggcttt catcaacaaa tcgccaaggc aatgcgcttc tttagtgacc
    17281 agaatcacta tccggcgacg accggcagga ttcagctcac gcacggagcc ttctggcaat
    17341 gcgctatcga gatccgccag cagggtggaa tcattaaaaa tcccttccag ttccgtgcgc
    17401 ataaaaaagc gcccggtacg gtgatcaaca aattcattgt tctgtacgat atttaactcg
    17461 tgcttgtagc aaatattggt aatacgtgcg atcagacctt tttggtccgg acaaatagta
    17521 cgcagaactt tacgttggag tgaatgcatt gctggaaaaa ccttgttgag agtgtttgct
    17581 aaaccgtacc gtcaaccatt attggccgca gcacttttta aattttttac ctgaaccaca
    17641 agggcaggga tcgttgcgac caaactgcgg acgtgtaccg tcaatatagt accattgacc
    17701 gttttctttc aaaaatcgcg aacgttcaat tattgcacct gttttgccac cttcggtaaa
    17761 tcgagcaaca aaactgacaa atccgatatt gtcggcgtcc tgccaacaat gttcaaatac
    17821 ggtcaatccc aaccattcag tatgcgcaaa tccggccatc aattcggcac gtaacgccgc
    17881 tgctccacaa gagggatgcc aggtctttat taaataatct gcgtcttgca tcacaaaagc
    17941 gcagtaacgc gaacgcatga gatgttctgg atcaggtgca accttttcac cagacacata
    18001 agggtggcaa cataggctat actcgacagc actaccacag ggacaaagct gagacacaaa
    18061 taatctccct ggaaacaata acggcgtatt aaccgcctga gtagcactat gttaaccgag
    18121 cagtagcgat gtggctacga ttgcattcca ggggaatctt gcgggaataa tgagaaagat
    18181 aaaaataggg ctggcgctgg gatctggcgc ggcgagaggt tggtcgcata ttggcgttat
    18241 taatgcgcta aaaaaagtgg gtattgaaat tgatatcgtt gcaggatgtt caattggttc
    18301 gctggtgggc gctgcctatg catgcgatcg attatctgcg ctggaagatt gggtgacctc
    18361 tttcagttat tgggatgttt tacgcctgat ggatctctcc tggcagcgcg gtgggttact
    18421 gcgcggcgag cgtgtcttca atcaatgccg cgaaataatg ccggaaacag agatcgaaaa
    18481 ttgttcccgt cgctttgcgg ctgttgccac caatttaagt acgggacgtg aattatggtt
    18541 tactgaaggc gatctccatc ttgctattcg cgcatcatgc agtattccag gactcatggc
    18601 acctgttgca cataacggct actggctggt tgatggagca gtagttaacc caattcctat
    18661 ttccctcacg cgtgcattgg gggctgatat tgtgatagcg gtcgacctgc agcacgatgc
    18721 tcatttgatg caacaagatt tgctctcctt taatgtcagt gaagaaaata gcgagaatgg
    18781 tgaatctttg ccgtggcatg cgcgtctgaa agaaaggtta ggcagtataa caacacgtcg
    18841 ggcggtgaca gcgccaacgg caacagagat tatgaccact tctatccagg tgctggagaa
    18901 ccgccttaaa aggaaccgca tggcaggtga tccgcccgat attctgattc aacctgtttg
    18961 cccgcaaata tctacgcttg atttccatcg cgcgcacgct gccattgcgg ccggacagct
    19021 ggcagtggaa aggaaaatgg acgaactttt gccgttggta cgcaccaaca tttgaccaga
    19081 atttttatct acacttaagt taattctgac aggcgcaggt ggcaatagca tgccactatt
    19141 gagtaaagcc agtcagggga gagaacatga cgcagccatt ggtcggaaaa cagattctca
    19201 ttgttgaaga tgagcaggta tttcgctcgc ttctggattc atggttttcc tcattgggag
    19261 cgacaacggt actggcggct gatggggtgg atgcccttga gttgctggga ggtttcactc
    19321 cagacctgat gatatgtgat atcgcgatgc cacgaatgaa cgggcttaaa ctgctggagc
    19381 atatacgtaa cagaggcgac cagaccccag ttctggtgat atctgccact gaaaatatgg
    19441 cagatattgc caaagcgtta cgtctgggcg ttgaagatgt tttgctgaaa ccagttaaag
    19501 atctgaatcg cttgcgcgag atggtttttg cctgtctcta tcccagcatg tttaattcgc
    19561 gcgttgagga agaggaaagg ctttttcgcg attgggatgc aacggttgat aaccctgccg
    19621 cagcggcgaa attattacag gaactacaac cgccggttca gcaggtgatt tcccattgcc
    19681 gggttaatta tcgtcaattg gttgccgcgg acaaacccgg cctggtgctt gatattgccg
    19741 cactttcgga aaacgatctg gcattttatt gccttgatgt cacccgagct ggacataatg
    19801 gcgtacttgc tgccttgtta ttacgcgcat tgtttaacgg attattacag gaacagcttg
    19861 cacaccaaaa tcaacggttg ccagagctgg gcgcgttatt gaagcaggta aaccatttac
    19921 tccgtcaggc caatctgccg gggcagtttc cgctattagt tggctattat catcgcgaac
    19981 tgaaaaatct cattctggtt tctgcgggtc tgaatgcgac gttaaatacc ggcgaacacc
    20041 aggtgcaaat cagtaatggt gttccgttag gcactttagg taacgcttat ttgaatcaat
    20101 tgagccagcg atgcgatgcc tggcaatgcc aaatatgggg aaccggtggt cgactgcgct
    20161 tgatgttgtc tgcagaatga gcaaacgata acgcggacta aatttgcatt acctgctaat
    20221 gtcggctggt ggtactatcg tcgccattcg tataagtaat tgtcttaatt atgctaactc
    20281 gcctcctttt cagaacttag ccccttcggg gtgctgatat actgggatgc gatacagaaa
    20341 tatgaacacg ttcaaaacac gaacagtcca ggagaattta aatggctgcc attaatacga
    20401 aagtcaaaaa agccgttatc cccgttgcgg gattaggaac caggatgttg ccggcgacga
    20461 aagccatccc gaaagagatg ctgccacttg tcgataagcc attaattcaa tacgtcgtga
    20521 atgaatgtat tgcggctggc attactgaaa ttgtgctggt tacgcactca tctaaaaact
    20581 cgattgaaaa ccactttgat accagttttg aactggaagc aatgctggaa aaacgtgtaa
    20641 aacgtcaact gcttgatgaa gtgcagtcta tttgtccgcc gcacgtgact attatgcaag
    20701 ttcgtcaggg gctggcgaaa ggcctgggac acgcggtatt gtgtgctcac ccggtagtgg
    20761 gtgatgaacc ggtagctgtt attttgcctg atgttattct ggatgaatat gaatccgatt
    20821 tgtcacagga taacctggca gagatgatcc gccgctttga tgaaacgggt catagccaga
    20881 tcatggttga acaggttgct gatgtgaccg catatggcgt tgtggattgc aaaggcgttg
    20941 aattagcgcc gggtgaaagt gtaccgatgg ttggtgtggt agaaaaaccg aaagcggatg
    21001 ttgcgccgtc taatctcgct attgtgggtc gttacgtact tagcgcggat atttggccgt
    21061 tgctggcaaa aacccctccg ggagctggtg atgaaattca gctcaccgac gcaattgata
    21121 tgctgatcga aaaagaaacg gtggaagcct atcatatgaa agggaagagc catgactgcg
    21181 gtaataaatt aggttacatg caggccttcg ttgaatacgg tattcgtcat aacacccttg
    21241 gcacggaatt taaagcctgg cttgaagaag agatgggcat taagaagtaa catccatatc
    21301 ggtgttatcc acgaaacggc gttgaacaat cgacgccgtt tttttatagc ttattcttat
    21361 taaattgtct taaaccggac aataaaaaat cccgccgatg gcgggatttt tagcaattgc
    21421 aatctacaaa agattattgc ttgatcagga aatcgtcgag ggatttacct tgctcatcca
    21481 ttgctttttt gattacagct ggagtacggc cctggccagt ccaggtttta gtttcgccgt
    21541 tttcgtcaac atagctatat tttgccggac gctgagcacg tctagctttg gtgccggatt
    21601 taacggcagc aaggctattc agcagttcgt tcgggtcaat accgtcagcg atcagcattt
    21661 cgcgatattg ctgcagttta cgagtgcgct cttcaacttc agcagcagcc gcgctttctt
    21721 cttcgcgacg ttcgttaaca acaacttcta atttttccag catttcttcc agcgtttcaa
    21781 gtgtacattc tcttgcctgc gcacgaagag tacggatgtt gttcagaatt ttaagtgctt
    21841 cgctcattgt agtaatctca aacttatatt ggggtggttt gttgaggtaa taatagagcc
    21901 ttaaattcag ttgtgcaata gccaggaatg taaggaattc aaaattgttc tttattttgt
    21961 gccgccaata aatatctttt cataaaatta gccagaaaag acgcggcata tagccctatt
    22021 tacaccgatg atttcgcagc acgtgaggtt aaaacttcct gattcatgtc acattttatg
    22081 gggagattat cgtaggctga cggcctttca gtcttctgta ttagttgtgt ttacgagaat
    22141 tccctattaa gcgaatgatg aaaagtagaa cagtcgcaat aagagcatgg acttagtatt
    22201 gcactatctc ctggaggtca acagagggct attacttgcg caacaggtta aagattgtga
    22261 atagttacca gcagtcattt acccgcttat aacaagcgag gcagttgtaa tgatagctca
    22321 gaaggattat gcaaggcttc gtaagggaga acgcatatac ccacttctgt gcatactgtt
    22381 gagctgaaaa actgacgaat tatgataaac tccagccaac tttatttcat atcattgagg
    22441 gcctgtggct gatggcacag ctatatttct actattccgc aatgaatgcg ggtaagtcta
    22501 cagcattgtt gcaatcttca tacaattacc aagaacgcgg catgcgcact gtcgtatata
    22561 cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag ttcgcgtata ggtttgtcat
    22621 cgcctgcaaa attatttaac caaaattcat cattatttga tgagattcgt gcggaacatg
    22681 aacagcaggc aattcattgc gtactggttg atgaatgcca gtttttaacc agacaacaag
    22741 tatatgaatt atcggaggtt gtcgatcaac tcgatatacc cgtactttgt tatggtttac
    22801 gtaccgattt tcgaggtgaa ttatttattg gcagccaata cttactggca tggtccgaca
    22861 aactggttga attaaaaacc atctgttttt gtggccgtaa agcaagcatg gtgctgcgtc
    22921 ttgatcaagc aggcagacct tataacgaag gtgagcaggt ggtaattggt ggtaatgaac
    22981 gatacgtttc tgtatgccgt aaacactata aagaggcgtt acaagtcggc tcattaacgg
    23041 ctattcagga aaggcatcgc cacgattaat aagaatttct ttactgacag ggtgagcagg
    23101 gcacttttat cctgtcagtt cgttttacgc acttcttccg ggctatatac ccttctcggc
    23161 agttttttaa cgccgctata cgcctcacag ggctcttaag caccgacgtt gacttgtgac
    23221 ctgtaaagta caatatccct gtgtttaggc gttatacatc gtcgcaaata tgatgaaggc
    23281 taatgctgtc ggtttatgga aaagttgctt tgggtaaaca aaaaatacgg ccccagaagg
    23341 gcaatgccgt tcacttaaga ggagcggcac tatgtttcac aggataacgg gtttttgata
    23401 tcttaaccga cctcggcctt gatggtcggg ggcgttttgt tatgaacacc acttccagag
    23461 caccccgaag atgctccagt cgtttcggga tggtccctgg tgacgccgtg tttcccagct
    23521 ctatcatttc tgatgcgata ttcttccacg caggcagtag ccagtggcgg ttacaaccct
    23581 tctggttcag cgttagcagc aggtcttcgc tgtaaaaaag cttatcaaac aacgtaatag
    23641 agttatccgg gatggtggcg agcatggagt gggccagcac agtttcgctc tgccggtaag
    23701 gtgcggtcac ggcattcagc agaatgtgac ttcccaggtt cattaaggcc accagacgca
    23761 taaccgggta ggcgttctgc cgcttagtgg atgtgttggc agacccataa tattcacgca
    23821 gctcgggttt atcaggtgtc ctgaactgtg cgccatcaat ggcaaaaagt tgcaggccgt
    23881 gccagtcatc cttcaggtaa cgttccgcgc ccctgtctgt gcggtctggc ggaagagcca
    23941 ttccactggg gcggccccca cgcgctgacg cgcttgggtg acagcgctgc gggccagcag
    24001 gttcatcccc gcttcgccat ccgcgctcag gttcagacgg cgaacaacat cggtaattgg
    24061 ctcattgcac caccatccag ataaccatgt cccccggtaa acgacggcgg cgaacggtcg
    24121 catgagcaga aagcgtcagg cagtgttgta tccactcggt gggaaggtgt tctgcaaata
    24181 gttgtgcaga gggcggaggc ataagcggat ggtcactgaa atcgagcaga tcattgagaa
    24241 gtggcataag aaaacggctc cctgttgtgg aagccgttat agtgcctcag tttaaggatc
    24301 ggtcaactaa tccttaactg atcggcattg cccagaaggg gccgtttatg ttgccagaca
    24361 gcgctactga ttaagcggat tttttcgctt ttttctcagc tttagccgga gcagcttctt
    24421 tcttcgctgc agtttcacct tctacataat cacgaccgta gtaggtatcc agcagaatct
    24481 gtttcagctc ggagatcagc gggtaacgcg ggttagcgcc ggtgcactgg tcatcgaatg
    24541 catcttcaga cagtttatcc acgttcgcca ggaagtctgc ttcctgaacg ccagcttcac
    24601 ggatagattt cggaataccc agttcagctt tcagcgtttc cagccatgcc agcagtttct
    24661 cgatcttagc agcagtacgg tcgcccggtg cgctcagacc caggtggtca gcaatttcag
    24721 cataacgacg gcgagcctgc ggacggtcat actggctgaa tgcagtctgc ttggtcgggt
    24781 tgtcgttcgc gttgtagcga ataacgttac aaatcagcag ggcgtttgcc agaccgtgcg
    24841 gaatatggaa ctgggaaccc agtttgtgcg ccattgagtg acatacaccc aggaaggcgt
    24901 tcgcaaacgc gatacccgcg atagtcgctg cactgtgaac acgttcacgc gctaccggat
    24961 ttttagaccc ttcgtggtag gacgctggca gatattcttt cagcagtttc agtgcctgca
    25021 gagcctgacc atcagagaac tcagatgcca gtacagaaac ataagcttcc atggcgtgag
    25081 ttactgcgtc cagaccaccg aaagcacaca gggacttcgg catgtccata accaggttgg
    25141 cgtcgacaat cgccatatcc ggagtcagcg catagtctgc cagcggatat ttctgaccag
    25201 tagcgtcgtc agttacaacc gcaaacggag tgacttcaga acctgtacca gaagtggtgg
    25261 tgacagcgat cattttcgct ttcacgccca ttttcgggaa cttgtagata cgtttacgga
    25321 tatccataaa gcgcagcgcc agctcttcga agtgagtttc cggatgttcg tacataaccc
    25381 acatgatctt cgcggcgtcc atcggggaac caccacccag cgcgataatc acgtctggtt
    25441 tgaaggagtt tgccagttct gcacctttac gaacgatgct cagggtcggg tccgcttcta
    25501 cttcgaagaa gacttcagtt tcaacgcctg ctgctttcag tacggaagtg atctgatcag
    25561 cataaccatt gttgaacagg aagcggtcag tcacgatgag cgcacgtttg tggccatcag
    25621 taatcacttc atccagcgcg attggcaggg agccacggcg gaagtagata gatttcggaa
    25681 gtttgtgcca caacatgttt tcagctcgct tagcaacggt tttcttgttg atcaggtgtt
    25741 tcggaccaac gttttcagag atggagttac caccccaaga accacaaccc agagtcaggg
    25801 aaggtgcgag tttgaagtta tacaggtcac cgataccacc ctgagacgct ggggtgttaa
    25861 tcaggatacg agccgttttc attttctgac cgaagtaaga aacgcgagcc ggttggttat
    25921 cctggtcagt gtacaggcaa gaggtatgac cgataccgcc catagcaacc agtttctctg
    25981 ctttttctac cgcgtcttcg aaatctttag cgcggtacat tgccagagtc ggggacagtt
    26041 tttcatgtgc gaacggttcg ctttcatcaa caacggtcac ttcaccgatc agaatcttgg
    26101 tgttttctgg tacagagaag cctgccagtt cagcaatttt ataggctggc tgaccaacga
    26161 tagccgcgtt cagcgcaccg tttttcagga taacatcctg aacagctttc agctctttac
    26221 cctgcaacag atagccgccg tgggttgcaa aacgttcacg tacagcgtca taaacagagt
    26281 caacaacaac aacagactgt tcagaagcac agattacgcc gttgtcgaag gttttggaca
    26341 tcagtacaga tgcaactgca cgtttgatat cagcagtttc atcgataaca actggagtgt
    26401 tgcccgcgcc tacaccgata gctggtttac cggagctgta tgcggcttta accatgcccg
    26461 gaccaccagt cgcgaggatc aggttgatgt ctgggtggtg catcagtgcg ttagacagtt
    26521 caacagaagg ttgatcgatc cagccgatca gatctttcgg agcaccggca gcgatagcag
    26581 cctgcagaac gatatcagcc gctttgttgg tggcatcttt tgcacgcggg tgcggggaga
    26641 agataatggc gttacgggtc ttcagactga tcagcgattt gaagatagca gttgaagtcg
    26701 ggttagtggt cggaacgata ccgcaaataa taccgattgg ttcagcgata gtgatggtac
    26761 caaaagtgtc gtcttcagac agaataccac aggttttttc atctttatag gcgttgtaga
    26821 tatattcaga agcaaagtgg tttttgatca ctttatcttc gacgataccc atgccggatt
    26881 cggcaacggc cattttcgcg agtgggattc gagcatctgc agcagccaga gcggcggcgc
    26941 ggaagatttt gtctacttgc tcttgagtga aactggcata ttcacgctgg gctttcttta
    27001 cacgctctac gagtgcgtta agttcagcga cattagtaac agccataatg ctctcctgat
    27061 aatgttaaac ttttttagta aatcatctgc tcgaatacga gagtatagtc agtgcggtga
    27121 tgatttgctt aacctatgaa aatcaaaagc ttactcgcgc tcacactcac tgtgatttac
    27181 taaaagagtt taaacattag agttattatc tctaatgcgt cacttccagg tggcgtaagc
    27241 aagattactc acttctgggt actgattacg tgatccaaat caaatttttt gcaaagctga
    27301 cacctttcag catcgctttt cgccattata gctaacagtt aataaattgt agtatgattt
    27361 ggtggctaca ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta
    27421 agtagttagg aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct
    27481 tatttatggc taacattatc cggcttttgc ttcggagcta accgtgattc agaccttttt
    27541 tgattttccc gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg
    27601 gattattccc gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac
    27661 taaccttaca gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga
    27721 cacgattctg caactttttg gtatatcaat tgattcgttc cgtatcgccg ggggcatcct
    27781 ggtggtgacg atagcgatgt cgatgatcag cggcaagctt ggcgaggata aacagaacaa
    27841 gcaagaaaaa tcagaaaccg cggtacgtga aagcattggt gtggtgccat tggcgttgcc
    27901 gttgatggcg gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag
    27961 cattagctat ctgtttggtt tctttgtggc tattgcattg ttcgctttat gttgctgggg
    28021 attgttccgc atggcacctt ggctggtacg ggttttacgc cagaccggca tcaacgtgat
    28081 tacgcgtatt atggggctat tgctgatggc attggggatt gaatttatcg ttactggtat
    28141 taaggggatt ttccccggcc tgcttaatta attcctttca aatgaaacgg agctgccatg
    28201 ctccgtttac ttcgtcatta tttttacttt gttcccg
//
LOCUS       DAJVTY010000035        27919 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVTY010000035 DAJVTY010000000
VERSION     DAJVTY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27919
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.035"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..473)
                     /locus_tag="OQQ66_001393"
     CDS             complement(<1..473)
                     /locus_tag="OQQ66_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS1904374.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSN"
     gene            complement(651..1562)
                     /gene="gsiD"
                     /locus_tag="OQQ66_001394"
     CDS             complement(651..1562)
                     /gene="gsiD"
                     /locus_tag="OQQ66_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1904375.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1565..2485)
                     /gene="gsiC"
                     /locus_tag="OQQ66_001395"
     CDS             complement(1565..2485)
                     /gene="gsiC"
                     /locus_tag="OQQ66_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1904376.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2503..4041)
                     /gene="gsiB"
                     /locus_tag="OQQ66_001396"
     CDS             complement(2503..4041)
                     /gene="gsiB"
                     /locus_tag="OQQ66_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1904377.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4061..5932)
                     /gene="gsiA"
                     /locus_tag="OQQ66_001397"
     CDS             complement(4061..5932)
                     /gene="gsiA"
                     /locus_tag="OQQ66_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1904378.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5919..6884)
                     /gene="iaaA"
                     /locus_tag="OQQ66_001398"
     CDS             complement(5919..6884)
                     /gene="iaaA"
                     /locus_tag="OQQ66_001398"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1904379.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7088..8323
                     /gene="moeA"
                     /locus_tag="OQQ66_001399"
     CDS             7088..8323
                     /gene="moeA"
                     /locus_tag="OQQ66_001399"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1904380.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8323..9072
                     /gene="moeB"
                     /locus_tag="OQQ66_001400"
     CDS             8323..9072
                     /gene="moeB"
                     /locus_tag="OQQ66_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1904381.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9148..9810)
                     /gene="fsa"
                     /locus_tag="OQQ66_001401"
     CDS             complement(9148..9810)
                     /gene="fsa"
                     /locus_tag="OQQ66_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1904382.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9941..10840
                     /locus_tag="OQQ66_001402"
     CDS             9941..10840
                     /locus_tag="OQQ66_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1904383.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            10846..13278
                     /locus_tag="OQQ66_001403"
     CDS             10846..13278
                     /locus_tag="OQQ66_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1904384.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13424..14239
                     /gene="ybiV"
                     /locus_tag="OQQ66_001404"
     CDS             13424..14239
                     /gene="ybiV"
                     /locus_tag="OQQ66_001404"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1904385.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14391..15656
                     /locus_tag="OQQ66_001405"
     CDS             14391..15656
                     /locus_tag="OQQ66_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1904386.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15897..17489)
                     /locus_tag="OQQ66_001406"
     CDS             complement(15897..17489)
                     /locus_tag="OQQ66_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1904387.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17708..18628
                     /gene="ldtB"
                     /locus_tag="OQQ66_001407"
     CDS             17708..18628
                     /gene="ldtB"
                     /locus_tag="OQQ66_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1904388.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18687..19805)
                     /locus_tag="OQQ66_001408"
     CDS             complement(18687..19805)
                     /locus_tag="OQQ66_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1904389.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19802..20269)
                     /gene="mntR"
                     /locus_tag="OQQ66_001409"
     CDS             complement(19802..20269)
                     /gene="mntR"
                     /locus_tag="OQQ66_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1904390.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGTK"
     gene            20455..20583
                     /gene="mntS"
                     /locus_tag="OQQ66_001410"
     CDS             20455..20583
                     /gene="mntS"
                     /locus_tag="OQQ66_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1904391.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            20855..22438
                     /gene="opgE"
                     /locus_tag="OQQ66_001411"
     CDS             20855..22438
                     /gene="opgE"
                     /locus_tag="OQQ66_001411"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1904392.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22487..23002)
                     /gene="ompX"
                     /locus_tag="OQQ66_001412"
     CDS             complement(22487..23002)
                     /gene="ompX"
                     /locus_tag="OQQ66_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1904393.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23055..23120)
                     /locus_tag="OQQ66_001413"
     CDS             complement(23055..23120)
                     /locus_tag="OQQ66_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904394.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23355..24242
                     /gene="rhtA"
                     /locus_tag="OQQ66_001414"
     CDS             23355..24242
                     /gene="rhtA"
                     /locus_tag="OQQ66_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1904395.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24541..25044
                     /gene="dps"
                     /locus_tag="OQQ66_001415"
                     /gene_synonym="pexB"
     CDS             24541..25044
                     /gene="dps"
                     /locus_tag="OQQ66_001415"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1904396.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25448..26194
                     /gene="glnH"
                     /locus_tag="OQQ66_001416"
     CDS             25448..26194
                     /gene="glnH"
                     /locus_tag="OQQ66_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1904397.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26333..26992
                     /gene="glnP"
                     /locus_tag="OQQ66_001417"
     CDS             26333..26992
                     /gene="glnP"
                     /locus_tag="OQQ66_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1904398.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26989..27711
                     /gene="glnQ"
                     /locus_tag="OQQ66_001418"
     CDS             26989..27711
                     /gene="glnQ"
                     /locus_tag="OQQ66_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1904399.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6886 a         7478 c         6922 g         6633 t
ORIGIN      
        1 ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa atcggcaaaa
       61 ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg ataaacaacg
      121 attttatcat tcgccctccg tgaaaagaga taatatttat tatcatcaag taccagtccc
      181 accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg aatctcctct
      241 gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc aatatcttta
      301 aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg ttttaccgcg
      361 cgttccgata ccagttgata gttaatgatg aaaaaaagac caataaatga cagtgctgcc
      421 aataaaaata aaattatcag ccttatcttt atcttttcgt ataaactcag catgttaata
      481 catttaacaa aataactatc tgaacgctgt gccgctgcaa aaatagtccc ttgctgcggc
      541 tttttaacca aattacccga ttctacaaaa tcagcagaaa gttacaacac ttgcttaggg
      601 caataatttc ctgtgaggtg attacccttt caagcaatat tcaaacgtaa ctatccttta
      661 attttcggat ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc cagtacggtc
      721 agaaaaatag ccagggccgg aaaaacagcg acatgcggcg cgataaccat atccgctcgt
      781 gcctcattga gcattgctcc ccactctggt gtcggcggct gcgcaccgag gccgagaaat
      841 gacaggctgg cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa caccacgata
      901 gacgagacgg tcccaggcaa gatatgacgc aataaaatgg tcatatcgct ggcaccaata
      961 ctgcgtgctg actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc gcgcaccagt
     1021 cgggcaaacg cggggatgga aaaaatggcg actgcgataa tcacgttagc aatgccgctt
     1081 cccaacaccg caacaacagc gatcgccagt aaaatacccg ggaaggcaaa cagcacatcg
     1141 caaatgcgca tgatcagccg atcccaccag ccttcataat atccagccaa caagcccagc
     1201 aacgtcccga tcgccgcacc gataaacacg gcaaacacgc ccgccgccag cgagatttgc
     1261 gcaccaacca ggacacggct gaaaatgtcg cgccccagtg aatcgacgcc aaaccagtgc
     1321 tgcaaagaag gtccgttatt cagattgtca taatcaaaat aattttcggc gtcataggga
     1381 gcgatccagc gtgcaaaaat ggccaccaca atcaataaaa taacgaataa tgcggcggtc
     1441 atcgccatat gctgacggcg aaatcgtcgc cagaattcat gccacggtgt acgcacctgg
     1501 tcaggtttga ccagtggcat ggcgtttaac accgcctgac gtcgccagtt aaatagtcgc
     1561 atccttactt gtacctgata gccgggttaa tggcggcgta aagcacatcc accactaagt
     1621 tgataagaat aaattccagc gagaaaagca gaatttccgc ctgaatcacc gggtaatcgc
     1681 gcatttctac ggagtcaacg agtaagcgtc caagtccggg ccagttgaaa actttctcca
     1741 ccacgataga accaccgagc aaaaagccaa actgtaagcc catcatggtc actaccggga
     1801 tcatcgcgtt acgtagcccg tgtttgagga caacccaggt ttcgctcacc cctttcgccc
     1861 tcgcggtacg catataatct tcgcttaaaa catcgacaaa cgacgcgcgg gtaaagcgcg
     1921 ccatcacggc tgccactgcc gcgccgagcg tcagggagga taaaatgtag tgctgccaac
     1981 tgtctgctcc cacggtaggc agccagccca gttcaacgga gaatacctga attaaaagca
     2041 tccccagcgc aaatgccgga aacgagatcc ccgacaccgc aatggtcata ctcaatcgat
     2101 ccggccaacg attacgccag acggcggcga taattcccgc cgccatacca aatatcaccg
     2161 cccagaccat actggttatg gtcagccaca gcgttggcat aaagcggctg gcaatctcat
     2221 cggcaaccgg acgacgcgac accatcgaca ggccaaaatc cccctgcaca gcattgctga
     2281 tatagtgcca gaactggtga tacagcggct gatccaaccc cagctgctga cgcaccagtt
     2341 ctataacctg cgcatcagct tcgggcccgg caatcaatcg cgccggatcg ccgggcagca
     2401 tatgcacaaa taaaaacacc agcaccgaga cgataaacag cgtcggaatc aaccccagta
     2461 agcgtttgat aacgtaatta agcattccac tccctgcgtt gcttattgca aatccgcgtc
     2521 ttcaaagctg aagccggtgt ctggcatgat ccaaaaaccg gtcaggtttt tactgtgtgc
     2581 cgacaccagt ttttctacca ccagcgggat ccacggcgac tcttgccaga tgatatcctg
     2641 cgccgcctta tacaagcggg tcttttccgc cggatcatta gttttcagtg cctgagccag
     2701 gaagtcgtcc acctgtttat tgctgtaaaa tgcggtatta aacagcgtcg gtggccagtt
     2761 ttgcgaggcg aacagtggcg atagcgccca gtccgcttcg ccggttgaag ccgaccagcc
     2821 agtgtagaac atccgcacgc cgctctcttt ttgcccttta ccttcaactt ctgccgcccg
     2881 ctgtccggca tccatcgcgg tcacctgggc tttaatcccg acctgcgcta actgctgctg
     2941 ggtaaattgc agcactttct gcgcggtgct gtggttatgt gacgaccaca gcgtggtact
     3001 gaaaccgttg ggatatcccg cctctttcag taattcgcgc gctttcactg gatcgtaagg
     3061 ccacggttta taactttgcg cgtaggcgat acttggcggt accacaccag tagctggcgt
     3121 tgcatagccc gcaaaggcaa ctttcaccag cgccggacgg ttaatggcgt aattcagcgc
     3181 ctcacggacc ttcgggttat cgaacggctt ttgcgtcacg ttcatactga tataacgctg
     3241 cataattgac ggactggcca tcaactcgat atttttgttt ttctccagca gtgcggcctg
     3301 ctcgtaggga atggggaaag caaactgcgc ttcaccggtt tgcagcattg ccgcgcgggt
     3361 gttgttatcc gccaccggac gccaggttat gctgtccagt ttgggcaatc ctggctgcca
     3421 gtaacccgcg aattttttca ccttcacaaa atcggtctga ttccaggtat ccagttcata
     3481 cggtccggtt cccaccggat gaaaaccaat ctccttgcca tatttttcca gcgctgccgg
     3541 tgaaatcatc gcggtcgccg gatggacaag aatattaata aacgctgaga acggctgttt
     3601 gagggtaatc tttaccgttg tcggatcgat cgcctccgtt ttggcaatat tcttatacag
     3661 gttatagcgt ttaagatgat tcgccggatc gctggcccgg tccagattcg ctttcaccgc
     3721 cgcggcgttg aaatcggtgc catcctggaa tttaattcct tcccgcaatt tcacggtgta
     3781 agtaatgccg tcatcggaaa cggtataact ctccgccagc acgtttttca gtttcatctc
     3841 tttatccaga ccgaacagcc cctggtaaaa cgatttcgct acggcctgag ataacgtgtc
     3901 attcgcgtca tacggatcga gcgtggtgaa attcgatcct accgccacca ccacatcttt
     3961 ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca ctaacccact
     4021 acggtgtaca gctcttgcca ttttattctc cgcctgaatg ttatctacgc atgaatgcgt
     4081 attctgattg tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac gagacggctg
     4141 ccacctcttc gccacgcaga tgaatattgc tgggaagatc gtccgacagc agtacacgct
     4201 gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc cagtaattta cgcgtataag
     4261 gatgctgcgg gttttcgaag accgcgcgcc gtggaccaat ttcaacaatt tgcccgagat
     4321 acatcaccgc cacacgatga ctaatccgct ctaccacggc catatcgtgg gagataaaca
     4381 gatacgcaat gccgaaatca cgctggagat cgagcaacaa gttgataatc tgcccgcgaa
     4441 tagaaacatc cagcgccgaa acggcttcgt cggcaatgat cacttttgga ttcaatgcca
     4501 acgcgcgagc aatgcagatg cgctggcgct gaccgccgga aaactcatgc gggtaacgcc
     4561 aggcatgttc aggtaacagg cccacgcgct ccagcaacca cgcaacgcgt gcagccgcgt
     4621 ctttacctgg caataaaccg tgtacacaca gcggttcgat aatcgaatca ccgatggtct
     4681 gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa ctgaatatcc cggcgcaatg
     4741 cctgaagttt gccaggtgac aaggtatcga ttcgctgacc gttaaagata atttcgccgc
     4801 cctgcgattc gaccaggcgc agcaacgccc gcccggtagt ggatttaccg ctgccagact
     4861 cgcccaccag cgatagcgtt tcgccaggcc agagatcaaa actgactttc tcaacggcat
     4921 gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg gtgacaagat
     4981 tacgcactcg taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg gggggttcct
     5041 gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg gggataatct aaccctttca
     5101 tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt gtaaggatgt tgcggtgcat
     5161 gaaaaatctg ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc accagtaccc
     5221 gatcggctat ctctgccacc acgcccatat cgtgcgtgat aaagataaca cccatcgaca
     5281 tctctttttg caatactttg attaattgca ggatctgcgc ctgaatagtg acatccagcg
     5341 cggtggttgg ctcatcggca atcagcaccg ccgggcggca tgacagcgcc atcgcaatca
     5401 tcactcgctg gcgcatcccg ccagagagtt gatgcggata acgtgaaaga atggtttgtg
     5461 cctcaggaat gcgtacctga tccagcatcc gcttcgcctc gaccatcgct tcttcacgac
     5521 tggcgttctg atgcagacga attgattcgg caatctgttc acccacagta aataccgggt
     5581 tcagcgatgt catcggctcc tgaaatatca tcgccatatc cgcaccgcga acatggcgca
     5641 tttgtgcagc gctctgctcg ctaagttcaa tcacttcgcg actgcgccgc tgcaacagca
     5701 ttttatcgca ctgtactaaa ccgcccgcct gttccaacag gcgcatcaat gccaacgcag
     5761 tcactgactt accggagccg gattcgccaa caattgccag cgtctcaccg cgttgcagac
     5821 taaaagagag attgcggacc gcagctattt tctgctggtc ctgcataaag gcaatattca
     5881 gattttcaac cgccagcaca ttaccggcat caagttcatc actgtgtggc aacggtgtcc
     5941 cctttttcac ggtagatacc ggtggttggc gtatcgcctg cgtagcccca ggcgcgatac
     6001 attccttcgg tgttaaacgg tagcgcgaca ttcccttcat ggccgatagc gattaagcca
     6061 ccgctaccgc caagcgcagg gagtttttcc attactaccc gctcgcaggc ttccgcgaga
     6121 cttaatccgc cgtaatccat taacgcggcg atgtcatatg ccgccagcgc gcggatgaag
     6181 acttcgcccg tgccggtaca agaaaccgcc acactggcgt tattggcgta gcatccggca
     6241 cccactaagg gactatcgcc aactcgtccg ggtaatttat tggtcattcc gcctgtggac
     6301 gtggctgccg ccaaattgcc gtctaaatcc aacgccacgg cccccacggt gcccattttc
     6361 tgtttttcat ccagtggcgc accgctatgg tcgaggacgg ttgccccttc cttgcgcgct
     6421 gccagtagtt gttcataacg caaaggcgtg gagaaaatcg ccggcgagac gcgctccatg
     6481 ccacgagcaa acgcaaaatt ttctgcccct tcgccaatca tcatcacatg cgggctttgc
     6541 tccatcacca gccgggcggc aagaaccgga ttacgcagat gactaacgcc cgccaccgca
     6601 ccggctttca gggtgttacc atccatcaca caggcgtcca gttcatgggt ttcatcacgc
     6661 gtaaagacag cgccaattcc ggcgttaaac agtggacact cttccagcag acgcaccgct
     6721 tccgtcacca catccagcgc actttcgccc gcttccagca ttttctgccc ggtttcaaca
     6781 atggcagaca acgcctcaat gtagcgtaat tcctgttgca gactcatctg cgcgcggcta
     6841 attgcacctg cgccaccatg aattgcaatg actgctttgc ccatagtatt cgtccattaa
     6901 gggtggctcg ccacttttgc tataaatatc agaattttta tgaattactc attcaatttt
     6961 tgaatataga aagatggata tggcatgtaa aggcaggagc caacgctacg cccgaggtgg
     7021 catacagtct gtggcgtttt tctgacataa taggcaaatt cgattttgcc tccgcaggag
     7081 tgttttcatg gaatttacca ccggattgat gtcgctcgac accgcgctta atgagatgct
     7141 ttctcgcgtc accccactga ccgcccagga aacgctgcca ctggtacagt gttttggtcg
     7201 tattctggcg agcgatgtcg tttcgccgct tgatgttccg gggtttgata actccgcaat
     7261 ggacggctac gcggtgcgtt tagccgatat tgcctccggg caaccgctgc ccgttgccgg
     7321 taaatccttt gccggtcagc cgtatcatgg tgaatggcct gcgggcacct gcattcgtat
     7381 tatgaccggt gcgccggtgc cggaaggctg cgaagcggtg gtgatgcagg agcagactga
     7441 acaaacggac aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa atattcgccg
     7501 tcgcggtgaa gatatctctg caggtgcggt tgttttcccg gcgggaactc gcctgactac
     7561 cgcagagctg ccagtaattg cttcactggg gattgccgaa gttccggtga ttcgtaaagt
     7621 gcgtgtagcg cttttttcta ccggtgatga actccagttg cccggtcagc cgctgggtga
     7681 cggccaaatc tacgacacca accgtctcgc cgtacacctg atgttagaac agctgggttg
     7741 cgaggtaatc aacttaggga ttatccgcga cgatccccat gccctgcgcg ccgcatttat
     7801 tgaagccgac agccaggcgg atgtagtgat cagttccggc ggtgtttcag tgggtgaagc
     7861 ggattacacc aaaacgattc ttgaagagct gggggagatc gccttctgga agctggcgat
     7921 taaaccaggt aaaccgttcg cgttcggtaa actcagcaat agctggttct gcggcctgcc
     7981 gggcaacccg gtttcagcga cgctgacctt ctatcaactg gtacagcctt tgctggcaaa
     8041 actaagcggc aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca cagcgtcccg
     8101 cctgaaaaaa acgccaggac gtcttgattt ccagcgcggc gtgctgcaac gcaacgccga
     8161 cggtgaactt gaagtgacga ccaccggaca tcagggttca catatattta gctcctttag
     8221 tctcggcaac tgctttatcg tgctggaacg cgatcgcggc aatgtggacg tgggcgaatg
     8281 ggtggaagta gaaccgttta acgcgttgtt cggaggcctg taatggcgga actcagcgat
     8341 caggagatgc tgcgctacaa ccggcaaatc attctgcgcg gttttgattt tgacggtcag
     8401 gaggcgctaa aagattctcg tgtgctgatc gtcggcctcg gtggactcgg ctgcgcagct
     8461 tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt cgacacggtt
     8521 tcgctctcga atctgcaacg ccagacactg cacaatgatg ccacggtcgg gcaaccgaag
     8581 gtggaatccg cccgtgacgc cctgacgcgg atcaacccac atatcgcgat tacgccagtc
     8641 aatgcactgc tagatgacgc agaacttgca gcattgattg ctgaatacga tctggtgctc
     8701 gactgtacgg ataacgttgc ggtacgtaat caactgaacg caggctgttt tgccgcgaag
     8761 gtgccgctgg tttccggcgc ggcaattcgt atggaagggc aaatcacggt cttcacttat
     8821 caggacggcg aaccgtgcta tcgctgcctt agccgtttgt ttggcgagaa cgcgttaacc
     8881 tgcgtagaag caggcgtgat ggcaccgttg atcggcgtaa ttggttcgtt gcaggcaatg
     8941 gaagcgatca aactgctggc aggttatggc aaaccggcca gcgggaaaat cgtcatgtac
     9001 gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc gtaatccggg gtgtgaggtg
     9061 tgcgggcagt aattgccacc tccaggccag ataagacgcg gcactccgcc acgtcatcag
     9121 caatttatga ggatgcagaa cgtccggtta aatcgacgtt ctgccaaacg ctcccagcca
     9181 gtcctgttca aacttcgcca cagcggcatc aaccgccgga tagctaatca tctgttgtgc
     9241 cacatccagt ggcagcgtaa ttgattcaca tcctgccagt aagcagtcca gcgcctgacg
     9301 cgggtttttg aaactcgctg ccagcacttt cgcctgcggc gcatgcattt tcaataactg
     9361 gtgtaagtcg gtcacagtct gaatgccgct accgccctga gcatcaatac gattaacgta
     9421 aggcgcaaca tattccgcac ctgccagcgc cgacagcagc ccttgtgctg cgccatatac
     9481 cgcggttccc agcgttggaa tcccttccgc ttttaacatc ttaatagctg ccagcccctc
     9541 ggcggtcacc ggaactttca ccacgatatc cgcaataata gaacgcaatt tacgtgcgtc
     9601 attcaccatc ccttcagcag tggtagccat tacctgggca aacagacgcc cctgaccgcc
     9661 catcgcttca tgaagttgcg gaagcacaac atccagcggt tttttacccg cggcgataat
     9721 gcttgggtta gtggtcacac ccgccagcgg aaaaatacgt gacagcgcct tcactgcaac
     9781 aacgtctgaa gtatccagat acagttccat aaccatcctc aaaatgtact ttttgtctga
     9841 atgtagacta tcacgggaaa actctctgaa cagttgacgc acatcaatcc aactttcatt
     9901 cgaaagtatt ttaatgtttg aacgaaacag agaggcaact atgattttca atattcagcg
     9961 ctactcgacc catgatggcc ccggtatccg cacggtcgta tttcttaaag gctgttcgct
    10021 gggctgccgc tggtgtcaga acccggaaag ccgcgcccgc acgcaggatc tgctgtatga
    10081 cgcacgactg tgtctggaag gctgcgagct gtgcgctaag gcagcgccgg aagtgattga
    10141 gcgcgcattg aatggtttgc ttattcatcg ggaaaagtta accccggagc atctgacggc
    10201 gctaaccgac tgctgcccga cacaggcgtt aaccgtgtgt ggggaagtga aaagcgttga
    10261 ggagatcatg gcgaccgttc tgcgcgataa accgttttac gatcgcagcg gcggcggctt
    10321 aacgctttcg ggtggtgaac cctttatgca gccggaaata gcgacggcgc tactgcaagc
    10381 ctgccacgag gcaggcattc atactgcggt agaaacctgt ctgcatgtgc cgtggaaata
    10441 tatcacccct tctctgccct atatcgatct gtttcttgcc gatttaaaac acattgccga
    10501 cgcgccgttt aaacagtgga ccgacggtaa cgccgccaag gtgctggata acctgaaaaa
    10561 actcgccgca gcgggcaaaa aaatcattat ccgcgtgccg ctgattcagg gctttaatgc
    10621 cgacgaaacc tctgtaaaag ccattaccga ttttgccgcc gacgagctgc acgttggcga
    10681 aattcatttt ctgccctacc acacgctggg catcaacaaa tatcacttac ttaatctgcc
    10741 ctatgacgcc ccggaaaaac cgcttgatgc gccagacctg ctcgactttg cccagcagta
    10801 tgcctgccag aaagggttaa ccgcgacctt acgaggataa caaccatgac cacactgaaa
    10861 ctggacacgc tcagcgaccg cattaaagcg cacaaaaatg cgctggtgca tattgtgaaa
    10921 ccgccggtct gtaccgagcg cgcgcagcac tataccgaga tgtatcaaca acatctcgat
    10981 aagccgatcc cggtacgtcg cgcgctggct ctggcgcatc acctggcgaa tcgcaccatc
    11041 tggatcaagc acgatgagtt gatcatcggc aaccaggcaa gcgaggttcg cgctgcgccg
    11101 atcttcccgg aatataccgt ctcgtggatc gaaaaagaga ttgatgatct ggcagatcgt
    11161 cccggtgctg gttttgcggt gagcgaagag aacaaacgcg ttctgcatga agtgtgcccg
    11221 tggtggcgcg gtcagacggt acaggatcgc tgctacggca tgtttaccga tgagcaaaaa
    11281 ggtctgctgg cgaccggaat cattaaagcg gaaggcaata tgacctccgg cgatgcgcac
    11341 ctggcggtga atttcccgct gctgctggaa aaaggtcttg atggtctgcg cgagaaagta
    11401 gcggaacgtc gctcgcgcat caatctgacg gtactggaag atttgcacgg cgaacagttc
    11461 ctgaaagcga ttgatatcgt gctggtggca gtcagcgaac acattgaacg ttttgcagcc
    11521 ctggcgcgtg aaatggccgc gaccgaaacc cgcgaaagcc gtcgcgatga actgctggcg
    11581 atagcagaaa actgcgatct tatcgcccac cagccgccgc agactttctg gcaggcgctg
    11641 caactgtgtt acttcatcca gttgatattg cagatcgaat ctaacggtca ctcggtatcg
    11701 tttggtcgta tggaccagta tctctacccg tactatcgcc gagacgttga actcaaccag
    11761 acactggatc gcgaacacgc catcgaattg ctgcatagct gctggctgaa actgctggaa
    11821 gtgaacaaga tccgctccgg ctcacactca aaagcctctg cgggaagtcc tctgtatcag
    11881 aacgtcacca ttggcggaca aactctggtt gatggtcaac caatggacgc ggtgaatcca
    11941 ctctcttacg cgatcctcga atcctgcggt cgcctgcgtt ccactcagcc taacctcagc
    12001 gtgcgttacc atgcaggaat gagtaatgat ttcctcgacg cctgcgtaca ggtgatccgt
    12061 tgcggcttcg ggatgccggc gttcaacaac gacgaaatcg tgatcccgga atttattaaa
    12121 ctcggtattg aaccgcagga cgcttacgac tacgcagcga ttggttgtat cgaaaccgcc
    12181 gtcggtggca aatggggcta tcgctgtacc ggcatgagct ttatcaactt cgcccgcgtg
    12241 atgctggcgg cgctggaagg cgggcgtgat gccacaagcg gcaacgtgtt cctgccacaa
    12301 gaaaaagcgt tgtcggcagg caactttaac aatttcgatg aagtaatgga cgcgtgggat
    12361 acgcaaatcc gttattacac gcgtaaatca atcgaaatcg aatatgtcgt cgacaccatg
    12421 ctggaagaga acgtgcacga tattctctgc tcggcgctgg tggatgactg tattgagcga
    12481 gcgaaaagta tcaagcaagg cggcgcgaaa tatgactggg tttctggcct gcaggttggc
    12541 attgccaacc tcggcaacag cctggcggca gtgaagaaac tggtatttga acagggcgcg
    12601 attggtcagc aacagctggc tgccgcactg gcggatgact tcgatggcct gactcatgag
    12661 caactgcgtc agcggctgat taacggtgcg ccgaagtacg gcaacgacga tgatactgtc
    12721 gatacgctgc tggctcgcgc ttatcagacc tatatcgacg aactgaaaca gtaccataat
    12781 ccgcgctacg gtcgtggtcc ggttggcggc aactattacg cgggtacgtc atctatctcc
    12841 gctaacgtac cgtttggtgc gcaaactatg gcaacgccgg acgggcgtaa agcccacacc
    12901 ccgctggcag aaggcgctag tccggcctct ggtactgacc atctcggccc tactgcggtc
    12961 attggctcgg tgggtaaact gcctacggca gcgattctcg gcggcgtgtt gctcaaccag
    13021 aaactgaatc cggcaacgct ggagaacgaa tctgacaagc agaagctgat gatcctgctg
    13081 cgcaccttct tcgaggtgca taaaggctgg catattcagt acaacatcgt ttcccgcgaa
    13141 acgctgctgg aggcgaaaaa acatccggat cagtaccgcg atctggtagt gcgtgtcgcg
    13201 ggttattccg ccttcttcac cgcgctctct ccagacgctc aggacgatat catcgctcgt
    13261 actgaacata tgctgtaatc tcttcggccc ggctgcggcg tcgccgggct aaatcattca
    13321 catcatcaat ttcatcctta ctttcattcg aaatataatt tgtgctctgc gtcacattgt
    13381 tattacactg ttacggtttc attttcgagc caggagcaca cctatgagcg taaaagttat
    13441 cgtcacagac atggacggta cttttcttaa cgacgccaaa acgtacaacc aaccacgttt
    13501 tatggcgcaa tatcaggaac tgaaaaagcg cggcattgag ttcgttgtcg ccagcggtaa
    13561 tcagtattac cagcttattt cgttctttcc tgagctaaag gatgagatct cttttgtcgc
    13621 ggaaaacggc gcactggttt acgaacatgg caagcaactg ttccacggcg aactgacccg
    13681 acatgaatcg cggattgtta ttggcgagtt gctaaaagat aagcaactca attttgtcgc
    13741 ctgcggtctg caaagtgcat atgtcagcga aaatgccccc gaagcatttg tcgcactgat
    13801 ggcaaaacac taccatcgcc tgaaagctgt aaaagattat caggagattg acgacgtact
    13861 gttcaagttt tcgctcaacc tgccagatga acaaatcccg ttagtgatcg acaaactgca
    13921 catagcgttc gatggcatta tgaaacccgt caccagtggt tttggcttta tcgacctgat
    13981 tattcccggt ctacataaag caaacggtat ttcgcggtta ctgaaacgct gggatctgtc
    14041 accgcaaaat gtggtagcga ttggcgacag cggtaacgat gcggagatgc tgaaaatggc
    14101 gcgttattcc tttgcgatgg gcaatgctgc ggaaaacatt aaacaaatcg cccgttacgc
    14161 taccgatgat aataatcatg aaggcgcgct gaatgtgatt caggcggtgc tggataacac
    14221 atcccctttt aacagctgac ctctctaccg gagccgtgcg ctccggtttt cccttctttt
    14281 tacatcctgt acatcacatc aaatcattaa acttgcatta acttatttat aacttatatt
    14341 gtcacatgca aatcataatg gtttcgaatg aaagttaatg agaggtggtt atggcttcta
    14401 cttttaccag cgacacattg ccagccgatc acaaagcagc tatccgtcag atgaagcacg
    14461 cgctgcgggc gcagcttggc gacgtccagc agatctttaa tcagctaagc gatgacattg
    14521 ccacgcgagt ggctgaaatc aacgcactca aagcacaggg cgatgccgtc tggccggtgc
    14581 tgtcttatgc cgatatcaaa gcaggtcatg ttactgcaga gcagcgcgaa cagattaaac
    14641 gtcgcggttg tgcggtgata aaaggccatt tcccccgcga acaagcgcta ggctgggatc
    14701 agtcgatgct ggactatctg gaccgcaacc gctttgacga ggtctacaaa ggccccggcg
    14761 ataatttctt cgggacgctc agcacttcac gtcccgagat ttaccccatc tactggtcgc
    14821 agacgcaaat gcaggcccgc cagagtgaag aaatggcgaa tgcgcagtcg tttctcaatc
    14881 gtctgtggac atttgaaagt gatggaaagc aatggtttaa cccggatgtg agcgtcatct
    14941 accctgaccg tatccgccgc cgtccgcccg gaacgacctc caaaggtctt ggagcgcata
    15001 ccgactccgg ggcgctggaa cgctggctgc ttccagcgta tcagcgcgtt ttcgccaacg
    15061 tctttaatgg caatctggcg caatatgatc cctggcatgc ggcacatcgt acggaagttg
    15121 aagagtacac ggtggacaac accaccaaat gttccgtgtt tcggacattc cagggctgga
    15181 cagcgctctc tgatatgctg cctggtcagg ggttgctgca cgttgtgccc attcctgaag
    15241 ccatggcgta cgtactgtta cgtccgctgc ttgatgatgt gccggaggat gaactgtgcg
    15301 gcgtagcgcc cggaagagtg ttgccggtat cagagcaatg gcatccactg ttaattgagg
    15361 cgttaaccag cattccaaaa ctcgaggccg gagactccgt ctggtggcac tgcgacgtca
    15421 tccattccgt tgcccccgtt gaaaatcaac agggttgggg caacgtgatg tacattcctg
    15481 cggcaccgat gtgcgagaaa aatcttgcct acgcgcacaa ggtgaaggcc gcactggaaa
    15541 aaggcgcatc gccgggcgac ttcccgcacg aggactatga aacaaactgg gaaggacgct
    15601 ttacgcttgc cgacctcaac attcacggta agcgagcgtt gggcatggat gtttgagagt
    15661 taatggaagt gaattgatgt gggattaaga aaatcgattc gacgttactc agattgctga
    15721 caagtgcgct ttcttaatgc ctgatgcggc gttaacgcct tatctggcct acaaagtcct
    15781 gcagattcaa tatattacag gtactgcgta ggcctgataa gcgtagcgca tcaggctatt
    15841 ttgcatttgt catcagccgc cgtcaccccg acagtatcgg ggtgacggat aatcacttac
    15901 tcgatccctt tactacgcag gtaatcttcg taattaccgc taaagtcgat cacgcgttcc
    15961 ggggtgattt ccagaatgcg ggtcgccagg gagcttacga actcacggtc gtgtgaaaca
    16021 aagatcagcg tgccctgata cagttccagc gccatgttca gtgactcgat ggattccata
    16081 tccaggtggt tggtcggttc gtccatgatc agaatgttcg gcttctgcat cattaactta
    16141 ccaaacagca tccgcccttt ttccccaccg gaaagcactt tagctggctt tttgatgtcg
    16201 tcctggctga acagcaaacg accgagaatg ctgcgtaccg cctgctcgtc atcgccttcc
    16261 tgcttccact gactcatcca ttcgaacacg gtcagatcat tttcaaactc atattcgtgg
    16321 tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt ttacggtgcc gctgtccggt
    16381 tgcagatcgc ccaccagcgt tttcatcagc gttgatttac cgacgccgtt ggtacccagt
    16441 accgcaagtt tttcaccgac ctccagcagc aggttgagat ttttaaacag tggaccgtta
    16501 tcaaaccctt tggtcagacc ttccacttcc agcgcgttac ggaacagttt cttatcctgt
    16561 tcaaaacgga tgaacgggtt ctgacggctg gaggctttca cctcttccag tttgatttta
    16621 tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg aggcgttggc gctaaagcgg
    16681 ctaacgaaag attgcaactc agcaatctgc gctttcttct tggcgttatc ggccagcaga
    16741 cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt agttacccgg ataaacgcgc
    16801 agctcgccgt aatccagatc cgccatgtgg gtacagacca tgttaaggaa gtgacggtcg
    16861 tgcgagatga tgatcatggt gctgtcacgc tcgttcagca cctgttccag ccagcgaatg
    16921 gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga gaatatccgg atcagcaaac
    16981 agcgcctgcg ccagaagcac acgcagcttc cagccaggag caacttcact catcgggccg
    17041 tagtgctgtt ccactggaat tcccacgcca agcaacaatt caccggcgcg agcttccgca
    17101 gagtaaccgt ccatttcgcc gtatttaact tccagatcgg ccactttata gccgtcttcc
    17161 tcactcattt ccggcaaagc atagatgcgg tcacgctcct gcttcacttc ccacaactct
    17221 ttatgcccca tgatcaccgt atccagcaca gtgaactctt caaaggcaaa ctgatcctga
    17281 cgcagcttac caatgcgctc gttgggatcg agggaaacgt tacccaaagt cggctctaag
    17341 tcgccgccga ggatcttcat aaaggtggat ttaccactac cgttcgcgcc aatcaggccg
    17401 taacggttgc cgccgccaaa tttgacggaa atgttttcaa acaacggctt actgccgaac
    17461 tgcatggtga cgttactgga aactaacaca ggcgtatcct gaaaagagat atgacaaacc
    17521 gcgcattatg ccataagtct gaaagaagat cctgctcctc gcttaaggtc tacactcaag
    17581 ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca ctgtgagctg gaatgaactt
    17641 ataatgcgct tccaatactc taatattctc aacccaatgg cctgccaggc acaaaatctc
    17701 gcttaacatg aatatgaaat tgaaaacatt attcgcagcg gccttcgctg ttgtcggctt
    17761 ttgcagtacc gcctctgcgg taacttatcc tctgccaacc gacgggagtc gcctggttgg
    17821 tcagaatcag gtgatcacca ttcctgaagg taacactcag ccgctggagt attttgccgc
    17881 ggagtaccag atggggcttt ccaatatgat ggaagcgaac ccgggtgtgg ataccttcct
    17941 gccgaaaggc ggtactgtac tgaacattcc gcagcagctg atcctgccgg ataccgttca
    18001 tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat tactatccga aagggaccaa
    18061 caccgttatc gtgctgccga tcggcattgg tcagttaggc aaagatacgc ctatcaactg
    18121 gaccaccaaa gttgagcgta agaaagcagg cccgacctgg acgccgaccg caaaaatgca
    18181 cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc gttccggcag gtccggataa
    18241 cccgatgggt ctgtatgcac tctacatcgg tcgcctgtat gctatccatg gcaccaacgc
    18301 caacttcggt atcggcctgc gtgtaagtca tggttgtgtg cgtctgcgta acgaagacat
    18361 caaattcctg ttcgagaaag taccggtcgg tacccgcgta cagtttattg atgagccggt
    18421 aaaagcgacc accgagccag acggcagccg ttatattgaa gtccataacc cgctgtctac
    18481 caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt accctgacga agagcgtgca
    18541 gacagtgacc ggtcagccag atgttgacca ggttgttctt gatgaagcga ttaaaaaccg
    18601 ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt aaagcaaaag gcggactcat
    18661 aatccgcctt ttttatttgc cagaccctag ttggccggga gtataactaa caaaacatat
    18721 cccaccaacg ccgcccacaa cagcatcggt attgaataga gatggaaacg ccaccagatg
    18781 cggcgatcgt tcgccatacg tagcgcaatc aaacttgcca gcgatccggg taataaccca
    18841 aagccaccta cgtttaccgc ccatgccagt aataaagacg gcggcacata gttcagcaac
    18901 aatatggtac tcggcacgtt actgatcacc tgcgataaac cgattgccgt taaccataac
    18961 ccgggttcag atagatgact cacgttaccc aacacgcctt gcaacgctgg aagctgggtc
    19021 agtaaatgga cgtcgataaa catcgccata aacaccagca gcagcgtcca gtccacactg
    19081 agcaccacgc gacgtgccag cagcgcaaag cctgccgcca caatcaccag tccccacagc
    19141 tcttgtttga actccagcgc cgttagaaag acgatataca gccccagaca actccacacc
    19201 agccgcggtt tccactccgg tgtttgcacc cccgtatggt attgcagcac ctttccaggg
    19261 aaacaacacc agcacaggag caggagcgtc agcatcattg cgccagccag cggtgccatt
    19321 tgggcaataa atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg
    19381 ttgccaattg gcgtcagtag cgaaccagca ttgactgcca gcgcctcaaa aataatcaga
    19441 cgattaaccg ggatctcaca cagtcttttt agcgtgatag tcagcggaac aacaataaac
    19501 agcgcgacat cgttggtcag aaaggtagaa agcagcgccg ccgccagcac cataaacatc
    19561 gccagccgac gctccgtagc aaagcggcgc accattttgc gccccagcac atcaaaataa
    19621 ccgcttaact ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc
    19681 cagtcgatag cagcaggcca ggatttcggt gcaaagggca cgaaaaagct taatccgata
    19741 ccaacaagaa ttaataactg aaaaaaacga tcgccttgca gcgtgcgtaa aaaaggcagg
    19801 ctcatttggt accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa
    19861 catggtgctc catgccttcc gcgtcgcgac gggcgatttc cggactgacg cccaacacca
    19921 acaagaaatt ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt
    19981 ctgccgttaa aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagcc
    20041 gcttaagcat tttagccacc gtcggttgcg aaactcccag acgagcagcc atgtccacct
    20101 gacgagcttc ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa
    20161 gctcgcgccg atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca
    20221 ctaactgcgt cacttttttt gctgttggcg tacctgcgcg acgactcatt gtgcttcctc
    20281 attatggtga cgcatacata gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg
    20341 aaggtacaaa aaattaacgt tttagcaata gctatataat atagcctgtg ctatatctgt
    20401 atgtaatgca atcatccctc aaggatcgac gggattagca agtcaggagg tcttatgaat
    20461 gagttcaaga ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatactg
    20521 ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc ttccgataaa
    20581 tagcggcgtc gcggtacgcc gcttcactcc tgctttcatg caggcataac gcgttttggc
    20641 ctgaaaaacc ccactttttg tcggatttgc aatccccttc gcaaaagatt tgttcgtcag
    20701 tagttgacct gaacggcggc tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg
    20761 cggatgcgga cccctttcca ctcttcacgc actcttgcag gtattgaccc ttgacgccag
    20821 ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct
    20881 tgttacccgt agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt
    20941 aattaacctc ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat
    21001 tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtgtcagttc
    21061 gctggtggcg gcttgttatt tcccttttgc tcaggcctac ggcgcgccta atttcaatac
    21121 attgctggca ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc
    21181 gtggtacagc tacctggtcg gcttatttat ttttgcgctc ggcgtaatag caatcaggcg
    21241 aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc tggtattcag
    21301 tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc tggggtggcg tatttaaact
    21361 gaaagatact ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga
    21421 ggtgattgaa gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt
    21481 cactgccgtt aagccgaagt atcagaccta tgtggtggtg atcggtgaaa gcgcgcgtcg
    21541 cgatgccctc ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa
    21601 cggtttgata tttgctgact acattgccgc cagtggctcc acgcagaaat cgcttggctt
    21661 aacgctcaat cgcgtagtcg atggcaaacc acagtttcag gataactttg tcaccctggc
    21721 aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag ggccaaatcg gcgaatacga
    21781 tactgctatc gccagcatcg ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa
    21841 ttttgaagca gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct
    21901 ggcgcaagag cactcgcaac cgcagctgat tgttctgcat ctgatgggct cgcatccgca
    21961 ggcctgcgac aggacacaag gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg
    22021 ctatctctat accatgacgc aaacggacga tttactgcgc aagctgtacg atcagttacg
    22081 caacagcggc agcagcttct ctctggttta cttttctgac cacggtctgg cctttaaaga
    22141 gcgcggcaaa gacgtgcaat accttgccca tgatgataaa tatcagcaaa atttccaggt
    22201 gccttttatg gtcatttcca gcgacgataa agcgcatcgg gtgattaaag cccgccgctc
    22261 agccaatgac ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa
    22321 tatcaaatac ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca
    22381 gttacagaag gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa
    22441 acaaaaatcc gccccgagag gcggattttt tatatcacca aagtgattag aagcggtaac
    22501 caacaccggc aatccaggtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga
    22561 agtccagagc aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt
    22621 agtcgctggt gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca
    22681 cacccactac accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga
    22741 tgccgtagta ctggtttttg ttgtagtcac cagagcttgc agtacgactt ttctcggtgt
    22801 aagtgaaaga accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt
    22861 tgaaaccgcc cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta
    22921 cggtagaagt cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg
    22981 aaagacatgc aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt
    23041 aaatatatca aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc
    23101 atgtaacaga acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga
    23161 actgcgtaag tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag
    23221 actaatgatt ccatcaatcc tggcgcattt taatcaaaac gggggaaaat tttttcaaca
    23281 aatgctcaac cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat
    23341 ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca
    23401 tattgctcgt tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc
    23461 ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca
    23521 tcgcgttctt taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt
    23581 tttacggcgt ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac
    23641 cgctgggtat tgcggtggcg ctggagttca ccggaccact ggcggtggcg ctgttctctt
    23701 ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc
    23761 taccgctggg gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg
    23821 ccggggcttg ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc
    23881 ctgcgacggt ggcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc
    23941 ttcaggctgg tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta
    24001 ttctctcgac cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac
    24061 ggacatttgg tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt
    24121 tcctcggaga aacactgaca cccatacagc tgctggcgct cggcgctatc atcgcagctt
    24181 caatggggtc tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt
    24241 aaatttacat ttctgcatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct
    24301 acaccgtctt tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag
    24361 cagataaata agaattgttc ttatcaatat atctaactca ttgaatcttt attagttttg
    24421 tttttcacgc ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata
    24481 gccggtgcta tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt
    24541 atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat
    24601 gtctccgaca gcgagaaaaa agcaacagta gagttgctga atcgccaggt tatccagttt
    24661 attgatcttt ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt
    24721 gccgtacatg aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg
    24781 gcagaacgtg cagtgcagct gggcggtgta gctctgggga ccactcaagt tatcaacagc
    24841 aaaaccccgc tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagaa
    24901 ctggctgacc gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa
    24961 gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg
    25021 tttatcgagt ctaacatcga ataaatccat cgctgatggt gcagaacttt agtacccgat
    25081 aaaagcggct tcctgacagg aggccgtttt gttttgcagc ccacctcaac gcacttattt
    25141 agtgcatcca tctgctatct ccagctgatt aagtaaattt tttgtatcca catcatcaca
    25201 caatcgttac ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga
    25261 gtgcacaatt ttagcgcacc agattggtgc cccagaatgg tgcatcttca gggtattgcc
    25321 ctataaatcg tgcatcacgt ttttgccgca tctctaaaaa tcaatgagtt gcaaaactgg
    25381 cacgattttt tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg
    25441 aaatgctatg aagtctgtat taaaagtttc actggctgca ctgaccctgg tttttgcggt
    25501 ttcttctcat gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt
    25561 tgaatttaaa cagggcgata aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc
    25621 taaagagctg aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc
    25681 actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa
    25741 aaaagcgatc gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc
    25801 taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag
    25861 cggtactggc tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt
    25921 cccgaacatc gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca
    25981 cgatacgcca aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt
    26041 aggtgactct ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct
    26101 gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat
    26161 ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc tacacctgta aagcgcactg
    26221 gcagttccct ctcccctctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg
    26281 ccattaatta ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt
    26341 tgactggagt gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct
    26401 gtggatttcg gtcctcggtc tggctggcgg tctggtaatc ggattgctgg caggttttgc
    26461 acgcaccttc ggtggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg
    26521 cggcacacct atcgtcgtcc aggtgatgtt tatctatttc gccctgccga tggcgtttaa
    26581 cgacttacgc atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc
    26641 gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt cacaaaggtt ttcgtgaagc
    26701 aggactggcg ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc
    26761 actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc
    26821 gctgtttatt gttatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg
    26881 taacttccgc gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct
    26941 ggtgctgagc tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt
    27001 aaaaacgtct ccaagcactt tggcccaacc caggtgctgc acaatatcga tttgaacatt
    27061 gcccagggcg aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg
    27121 cgctgcatca acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag
    27181 gttaacgatc cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag
    27241 cagttttacc tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg tccgctacgc
    27301 gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc
    27361 ggtctggcag aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt
    27421 gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact
    27481 tccgctcttg acccggaact gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa
    27541 gaagggatga cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg
    27601 cggctgatct ttatcgacaa aggccggatt gcggaagatg gcaatccgca ggtgttgatc
    27661 aagaacccgc cgagccagcg cttgcaggaa tttttgcagc acgtctctta ataagacaca
    27721 ttgcctgatg cgctacgctt atcaggccta caggatatct ggcaacttat taaaattgca
    27781 tgaacttgta ggacggataa ggcgttcacg ccgcatccgg caaaaaagcc cgcacgttgt
    27841 cagcaatctg cttaatatcc cttcctccct ttcacccgaa agggaggcac accagattcc
    27901 tctcatttaa aatcgcccc
//
LOCUS       DAJVTY010000036        27522 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVTY010000036 DAJVTY010000000
VERSION     DAJVTY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.036"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..527
                     /locus_tag="OQQ66_001419"
     CDS             <1..527
                     /locus_tag="OQQ66_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1904400.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="OQQ66_001420"
     CDS             893..1960
                     /locus_tag="OQQ66_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1904401.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="OQQ66_001421"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="OQQ66_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1904402.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMSAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="OQQ66_001422"
     CDS             4692..5816
                     /locus_tag="OQQ66_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1904403.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6164
                     /locus_tag="OQQ66_001423"
     CDS             5889..6164
                     /locus_tag="OQQ66_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1904404.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6161..6520
                     /locus_tag="OQQ66_001424"
     CDS             6161..6520
                     /locus_tag="OQQ66_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1904405.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OQQ66_001425"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OQQ66_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1904406.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OQQ66_001426"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OQQ66_001426"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1904407.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OQQ66_001427"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OQQ66_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1904408.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OQQ66_001428"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OQQ66_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1904409.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OQQ66_001429"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OQQ66_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1904410.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OQQ66_001430"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OQQ66_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1904411.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OQQ66_001431"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OQQ66_001431"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1904412.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13751)
                     /locus_tag="OQQ66_001432"
                     /pseudo
     CDS             complement(12715..13751)
                     /locus_tag="OQQ66_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            13883..15100
                     /locus_tag="OQQ66_001433"
     CDS             13883..15100
                     /locus_tag="OQQ66_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1904413.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15104..15661)
                     /gene="dcrB"
                     /locus_tag="OQQ66_001434"
     CDS             complement(15104..15661)
                     /gene="dcrB"
                     /locus_tag="OQQ66_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1904414.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15734..16399)
                     /locus_tag="OQQ66_001435"
     CDS             complement(15734..16399)
                     /locus_tag="OQQ66_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1904415.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16620..16865
                     /gene="tusA"
                     /locus_tag="OQQ66_001436"
     CDS             16620..16865
                     /gene="tusA"
                     /locus_tag="OQQ66_001436"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1904416.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16967..19165)
                     /gene="zntA"
                     /locus_tag="OQQ66_001437"
     CDS             complement(16967..19165)
                     /gene="zntA"
                     /locus_tag="OQQ66_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1904417.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19239..19865)
                     /locus_tag="OQQ66_001438"
     CDS             complement(19239..19865)
                     /locus_tag="OQQ66_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1904418.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20006..20365
                     /locus_tag="OQQ66_001439"
     CDS             20006..20365
                     /locus_tag="OQQ66_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1904419.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20368..20637)
                     /locus_tag="OQQ66_001440"
     CDS             complement(20368..20637)
                     /locus_tag="OQQ66_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1904420.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20627..21223)
                     /gene="rsmD"
                     /locus_tag="OQQ66_001441"
     CDS             complement(20627..21223)
                     /gene="rsmD"
                     /locus_tag="OQQ66_001441"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1904421.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21373..22866
                     /gene="ftsY"
                     /locus_tag="OQQ66_001442"
     CDS             21373..22866
                     /gene="ftsY"
                     /locus_tag="OQQ66_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1904422.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22869..23537
                     /gene="ftsE"
                     /locus_tag="OQQ66_001443"
     CDS             22869..23537
                     /gene="ftsE"
                     /locus_tag="OQQ66_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1904423.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23530..24588
                     /gene="ftsX"
                     /locus_tag="OQQ66_001444"
     CDS             23530..24588
                     /gene="ftsX"
                     /locus_tag="OQQ66_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1904424.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24833..25687
                     /gene="rpoH"
                     /locus_tag="OQQ66_001445"
     CDS             24833..25687
                     /gene="rpoH"
                     /locus_tag="OQQ66_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1904425.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25958..27061
                     /gene="livJ"
                     /locus_tag="OQQ66_001446"
     CDS             25958..27061
                     /gene="livJ"
                     /locus_tag="OQQ66_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1904426.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27260..>27522)
                     /gene="panM"
                     /locus_tag="OQQ66_001447"
     CDS             complement(27260..>27522)
                     /gene="panM"
                     /locus_tag="OQQ66_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1904427.1"
                     /translation="ARFNERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEV
                     LRNNPGVSCWWMADAGVEDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6497 a         7168 c         7671 g         6186 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 agtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaactctatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata ttcccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcggttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggtgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     9541 atcagcgtaa agcactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagt gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataaccc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctagccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 caggcgatgg cccatcatcc gtggtcgatg accatatgga ccactaaaaa caatgcgccg
    12961 accagaatac atgcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
    13021 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
    13081 agcgcaaact gtacgcccat tagcgccagc cccagccaga cgatgacacc tgtccataaa
    13141 ctgagtagcg tctttaacgc cagataatgc gaaacgcctt taagtgcgcg gtgtaatcct
    13201 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
    13261 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
    13321 agcccgatca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
    13381 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
    13441 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
    13501 gccagtacgc cgactagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
    13561 gtaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
    13621 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
    13681 aggatcacta ccagcgaggc caacttgagc agaatgtgca tgcccgtttt atcgggttga
    13741 ggggtttcca ttggggcttc cttgtgactt tttgtgttaa gtgtagcggg agtcacgtca
    13801 gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa tgaaacactg
    13861 ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt
    13921 gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat
    13981 cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt
    14041 ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc
    14101 cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt tatgcggctg
    14161 ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc ccgtcatcag
    14221 cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac
    14281 gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gagtgatttc
    14341 gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt
    14401 ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc
    14461 cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt
    14521 tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg cttctgccgg
    14581 atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaagtt gggacggtgc
    14641 ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa
    14701 cggcattaac cgtatcggcg gcttaaacgt agcgatgatt tgctttagcg ttgagataat
    14761 cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg gcgtcttact
    14821 ggcgggggca gggttttcgc tggtgttccc ggcattaggt gtagtggcgg taaaagcggt
    14881 tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct
    14941 tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta
    15001 tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg
    15061 gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg
    15121 tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagcg ggcagcgtaa
    15181 tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct
    15241 ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg
    15301 ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct
    15361 tcgccagcac cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct
    15421 gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct
    15481 ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat
    15541 tcccggtagc gttattttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat
    15601 cacaggccgc aagccccata accagcaggc caattccgac atatttaacc agattgcgca
    15661 ttgacttctt cctttcgata aacggccata acggctcatt catccatctt atcacaactc
    15721 tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct gccagtcttt
    15781 tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca
    15841 ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg
    15901 ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta ccgaacagtg
    15961 tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta aaaacgtgca
    16021 cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa
    16081 acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag aatagcgacg
    16141 agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata cgacgggcca
    16201 gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac gggaagctaa
    16261 acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc agatagttac
    16321 tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag
    16381 tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa
    16441 tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt tcgagccgcc
    16501 gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg
    16561 tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa
    16621 tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg cgctgcccgg
    16681 aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa acgttgctga
    16741 ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc tttatggaac
    16801 acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg
    16861 gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca
    16921 gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca
    16981 acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc agcaccgcca
    17041 gccatagtcc ggtcatccct aaaagcgtgg tgacgaggaa gatccctttc agtcccagcg
    17101 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
    17161 ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc gccacgtctg
    17221 tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac
    17281 cgtcaccgac catcgccagc ggcgcatgtt gattcagctc ggtcaccgct ttgactttat
    17341 attccggcaa caggcccgct ttaaactcca gccccagctc cccggcaatt gccgccgctg
    17401 cgcgtggatt atcgccggtg aggatcaccc ctttgacgcc cagcgcgttc agttcactga
    17461 tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat
    17521 cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag
    17581 caaatgcatc agcgggatgt ttcccggcag cgcatatcaa cacgcgctca ccgttaacct
    17641 gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggcggga atggcgagtg
    17701 cagcaacctg tgcttcgcgc acgatggcct gcgccagtgg atgcgtcgcg ctttgctcga
    17761 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
    17821 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac
    17881 gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgctgccg
    17941 ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg cagccaatca
    18001 gcagcagcgt caggccttta taaatccact cctgccagct ggcggcaaac agcagcggtg
    18061 gcaccagcgt caccagcagg gcgacggcca taatcgctgg cgtataaata cggctgaaac
    18121 ggtcgataaa ccgctcaatg ggggcgcggc gctcttcggc ttcttcgatc agtttcagaa
    18181 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
    18241 cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc
    18301 cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
    18361 aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct
    18421 cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc
    18481 taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca
    18541 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta
    18601 cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgat
    18661 gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccgaacggat
    18721 gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta atcagcggca
    18781 gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat
    18841 agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg gcatcaacca
    18901 ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc tggcgcacgg
    18961 cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc cagctatagc
    19021 gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac
    19081 agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct
    19141 tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct cattaaccga
    19201 aggatacact ctggagtcga ctccagagtc aagttttatc agaaatacag tgagcggacg
    19261 atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt
    19321 cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca
    19381 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
    19441 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc
    19501 cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag
    19561 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
    19621 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
    19681 gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
    19741 gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
    19801 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
    19861 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
    19921 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctagcagggt atgcttattt
    19981 ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat
    20041 tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga
    20101 taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
    20161 gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta
    20221 tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
    20281 gcagtaccat gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt
    20341 tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
    20401 cagcagttca aacacgccga aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg
    20461 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
    20521 aaaaatcagc gccacgttaa cgaaaatatt cagcgggcgc gggaagggat gcaccagatt
    20581 gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac
    20641 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
    20701 gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg ctttcgacat
    20761 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
    20821 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta cctttttgcg
    20881 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
    20941 gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtg gcccccgcag
    21001 cgtagcgcga taacgcttcc agtcccagcg cgccgctccc ggcgaagcaa tccagacatt
    21061 gggcgtcaac aatgaccgga gccagccagt taaacaatgt ttcgcgtacg cggtcggtgg
    21121 tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
    21181 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
    21241 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
    21301 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
    21361 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
    21421 caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa
    21481 gaaatcgttc aggcgcaaga gcctgcgaag gcttctgaac aagccgtcga agagcaaccg
    21541 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa
    21601 caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca gccggaaccg
    21661 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccggaa
    21721 gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag
    21781 attgtcgaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa
    21841 gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtggttcc tccggtagaa
    21901 gagcagccgg tggaagaaat cgctcaggag caggaaaaac cgaccaaaga aggtttcttc
    21961 gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc
    22021 ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga acagctgttg
    22081 atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac ggaaggcgca
    22141 tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggc
    22201 gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc
    22261 ctgatggtgg gcgtcaacgg tgtgggtaaa actacgacga ttggtaagct ggcacgtcag
    22321 tttgagctgc agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg
    22381 gttgaacagc ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc
    22441 ggtgcggatt ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc
    22501 gacgtcctga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag
    22561 ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg
    22621 ctgactattg atgccagtac cgggcagaat gcggtaagcc aggccaaact gttccacgaa
    22681 gccgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta
    22741 attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt
    22801 attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag
    22861 gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc
    22921 tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg
    22981 gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga
    23041 aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc
    23101 gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgt actgtctacg
    23161 ataacgtggc gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg
    23221 tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc
    23281 tttccggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg
    23341 tactgctggc ggacgaaccg accggtaacc tggacgacgc gttgtcggaa gggattctac
    23401 gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca
    23461 acctgatttc acggcgttcc tatcgcatgc tcaccctgag cgatggtcac ttgcatggag
    23521 gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg tgggcgtctg
    23581 gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc accgaaacgc
    23641 gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc
    23701 tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg
    23761 gtgatggtta tcgccatctc cctgacgctg cccagcgtct gttatatggt gtacaaaaac
    23821 gttaatcagg cggcgacgca gtattatccg tcaccgcaaa tcactgttta cttgcaaaaa
    23881 acgctggacg atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg
    23941 gagaaagtga actatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt
    24001 tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc
    24061 ccgaaactcg attttcaggg gacggaatca ctcaatacgc tgcgtgatcg tatcacgcag
    24121 attaacggca ttgacgaagt gcggatggat gacagctggt ttgcccgtct ggcggcgttg
    24181 accgggctgg tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc
    24241 ctcgtcatcg gcaacagcgt acgtctgagc atctttgctc gccgtgactc catcaacgtg
    24301 cagaaactga ttggtgcgac ggatggtttc atccttcgcc cgttcctgta tggtggcgca
    24361 ctgctgggat tttctggcgc attgttgtca ttaattttgt cagaaattct ggtgctgcga
    24421 ttgtcatcgg cggttgcgga agtggcacag gttttcggaa cgaattttga tatcaatggt
    24481 ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca
    24541 gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt
    24601 atactctttc cctgcaatgg gttccgtagc agggaaagag atcccgttgt ctcttcccgg
    24661 tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt
    24721 ggataaaatc acggtctgat aaaacagtga atgataacct cgttgctctt aagctctggc
    24781 acagttgttg ctaccattga agcgccagaa gatatcgatt gagaggattt gaatgactga
    24841 caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc
    24901 taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta
    24961 ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca
    25021 tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa
    25081 catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc
    25141 cttcgccgtt cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat
    25201 cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaactaa
    25261 gcagcgtcta ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt
    25321 aaccagcaaa gacgttcgtg agatggaatc acgtatggcg gcacaggaca tgacctttga
    25381 tctgtcttcc gacgacgatt ccgacagcca gccgatggca ccggtgctct atctgcagga
    25441 taaatcatct aactttgccg acggcatcga agatgataac tgggaagagc aggcggcaaa
    25501 ccgtctgacc gacgcgatgc aaggtctgga cgaacgcagc caggacatca tccgcgcgcg
    25561 ctggctggac gaagacaaca agtccacgtt gcaggaactg gctgaccgtt acggtgtttc
    25621 cgctgaacgt gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg ctgctataga
    25681 agcgtaattt ccgctattaa gcagagaacc ctggatgaga gtccggggtt tttgtttttt
    25741 gggcctctac aataatcaat tccccctccg gcaaaacgtc aatccccacg cagattgtta
    25801 ataaactgtc aaaatagcta ttccaatatc ataaaaatgg ggtatgtttt agcagagtat
    25861 gctgctaaag cacgggtagt catgcataaa acgaaataaa gtgctaaatc acaacaccac
    25921 aacacacgta ataaccagaa gaatggggat tctcaggatg aatataaagg gtaaagcgtt
    25981 actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt
    26041 cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg
    26101 cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca
    26161 aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt
    26221 cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc
    26281 gtctgatatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga
    26341 gctgaccgcc cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg
    26401 gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca
    26461 cgacaaacag caatacggcg aaggtctggc gcgagcggtg caggacggcc tgaagaaagg
    26521 caatgctaac gtggtgttct ttgatggtat caccgccggg gaaaaagatt tctcaacgct
    26581 ggtggcgcgt ctgaaaaaag agaatatcga cttcgtttac tacggcggtt atcacccgga
    26641 aatggggcaa atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc
    26701 ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct
    26761 ggtgaccaag ccgaagaact acgatcaggt tccggcgaac aaacccattg ttgacgccat
    26821 caaagcgaaa aaacaggacc caagcggcgc attcgtttgg accacctacg ccgcgctgca
    26881 atctttgcag gcgggcctga atcagtctga cgatccggct gagatcgcca aatacctgaa
    26941 agcgaactcc gtggataccg tgatgggtcc gctgacgtgg gatgagaaag gtgatctgaa
    27001 aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcgaccg atgcgaagta
    27061 atcattaatc ggcaactttg ggttgccgcc aaattgctaa tatcgagtac gttgcttcat
    27121 gccggatgcg gcgtaaacgc cttatccggc ctacaagatc caaagaaatc agtaaattgc
    27181 aacacacatt gtaggcctga taagcgtagc gcatcaggca atttctcgtt ggtcatcact
    27241 tttgaaatca acttgacgat taacgcttct cccagccgcc ctgttgcgcc gtaaatccca
    27301 gtgcctgcat aaacgccgtc atcacaccgc gatcttccac gcctgcatcc gccatccacc
    27361 agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc cccacaccgc
    27421 gacgtcgggt gacttcccgc acacgcaggg aatccagtgc tccctcggtg ccgcttaagg
    27481 ttacccgtac ggcagcgagc aggcgctcgt taaaacgcgc gg
//
LOCUS       DAJVTY010000037        27424 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVTY010000037 DAJVTY010000000
VERSION     DAJVTY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27424
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.037"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..166)
                     /gene="fumD"
                     /locus_tag="OQQ66_001448"
     CDS             complement(<1..166)
                     /gene="fumD"
                     /locus_tag="OQQ66_001448"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1904428.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSEL"
     gene            723..2135
                     /gene="pykF"
                     /locus_tag="OQQ66_001449"
     CDS             723..2135
                     /gene="pykF"
                     /locus_tag="OQQ66_001449"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1904429.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2446..2682
                     /gene="lpp"
                     /locus_tag="OQQ66_001450"
     CDS             2446..2682
                     /gene="lpp"
                     /locus_tag="OQQ66_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1904430.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2746..3750)
                     /gene="ldtE"
                     /locus_tag="OQQ66_001451"
     CDS             complement(2746..3750)
                     /gene="ldtE"
                     /locus_tag="OQQ66_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1904431.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(3899..4315)
                     /gene="sufE"
                     /locus_tag="OQQ66_001452"
     CDS             complement(3899..4315)
                     /gene="sufE"
                     /locus_tag="OQQ66_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1904432.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4328..5548)
                     /gene="sufS"
                     /locus_tag="OQQ66_001453"
     CDS             complement(4328..5548)
                     /gene="sufS"
                     /locus_tag="OQQ66_001453"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1904433.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5545..6816)
                     /gene="sufD"
                     /locus_tag="OQQ66_001454"
     CDS             complement(5545..6816)
                     /gene="sufD"
                     /locus_tag="OQQ66_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1904434.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(6791..7537)
                     /gene="sufC"
                     /locus_tag="OQQ66_001455"
     CDS             complement(6791..7537)
                     /gene="sufC"
                     /locus_tag="OQQ66_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1904435.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7547..9034)
                     /gene="sufB"
                     /locus_tag="OQQ66_001456"
     CDS             complement(7547..9034)
                     /gene="sufB"
                     /locus_tag="OQQ66_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1904436.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9043..9411)
                     /gene="sufA"
                     /locus_tag="OQQ66_001457"
     CDS             complement(9043..9411)
                     /gene="sufA"
                     /locus_tag="OQQ66_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1904437.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(9959..10147)
                     /locus_tag="OQQ66_001458"
     CDS             complement(9959..10147)
                     /locus_tag="OQQ66_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1904438.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10247..10657)
                     /gene="menI"
                     /locus_tag="OQQ66_001459"
     CDS             complement(10247..10657)
                     /gene="menI"
                     /locus_tag="OQQ66_001459"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1904439.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10654..13710)
                     /locus_tag="OQQ66_001460"
     CDS             complement(10654..13710)
                     /locus_tag="OQQ66_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1904440.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14099..15211
                     /gene="ydiK"
                     /locus_tag="OQQ66_001461"
     CDS             14099..15211
                     /gene="ydiK"
                     /locus_tag="OQQ66_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1904441.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15397..15504
                     /gene="rprA"
                     /locus_tag="OQQ66_001462"
     ncRNA           15397..15504
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQQ66_001462"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15640..16019
                     /locus_tag="OQQ66_001463"
                     /pseudo
     CDS             15640..16019
                     /locus_tag="OQQ66_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            15964..16686
                     /locus_tag="OQQ66_001464"
                     /pseudo
     CDS             15964..16686
                     /locus_tag="OQQ66_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16698..17564
                     /gene="ydiB"
                     /locus_tag="OQQ66_001465"
                     /pseudo
     CDS             16698..17564
                     /gene="ydiB"
                     /locus_tag="OQQ66_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17596..18354
                     /gene="aroD"
                     /locus_tag="OQQ66_001466"
     CDS             17596..18354
                     /gene="aroD"
                     /locus_tag="OQQ66_001466"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1904442.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18500..20095
                     /locus_tag="OQQ66_001467"
     CDS             18500..20095
                     /locus_tag="OQQ66_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCS1904443.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20109..21260
                     /locus_tag="OQQ66_001468"
     CDS             20109..21260
                     /locus_tag="OQQ66_001468"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1904444.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21303..22214)
                     /locus_tag="OQQ66_001469"
     CDS             complement(21303..22214)
                     /locus_tag="OQQ66_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1904445.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22528..23299
                     /locus_tag="OQQ66_001470"
                     /pseudo
     CDS             22528..23299
                     /locus_tag="OQQ66_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23319..24257
                     /locus_tag="OQQ66_001471"
     CDS             23319..24257
                     /locus_tag="OQQ66_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCS1904446.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24313..25602
                     /locus_tag="OQQ66_001472"
     CDS             24313..25602
                     /locus_tag="OQQ66_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1904447.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25599..25892
                     /locus_tag="OQQ66_001473"
     CDS             25599..25892
                     /locus_tag="OQQ66_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1904448.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            25949..>27424
                     /gene="fadK"
                     /locus_tag="OQQ66_001474"
     CDS             25949..>27424
                     /gene="fadK"
                     /locus_tag="OQQ66_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1904449.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLE"
BASE COUNT         6819 a         6960 c         6764 g         6881 t
ORIGIN      
        1 ccagctcact gcgctgaatg cgtttattgg ctaatgctgt acgtaatacg cctgcgataa
       61 caatatgctt tggttcctgc cccagatcgc gcatttccag cacaacttta ccgacaactc
      121 tgcacatttc tcggtacagt tcatcctctt ttgtccgatt tcccattctt tatacctatt
      181 tatcatgcca actatcagca tatatcaatc taaccaatta aacaaaaatc aaacaaaatc
      241 agacaaataa cgcgataaat tattttaact gtagcaattg agcgatgata tatttataca
      301 ccggatgaac tttcacttat cctcacactg acaactttgg caccagacgt tgcgcaaaca
      361 gcgaagtttt tgcgtaacct tttccctgga acgttaaatc tttgataaca atttattgtc
      421 taacaagttg tatatttttt gaaacgctgt ttttgttttc cttttggatt aatttcagcg
      481 tataatgcgc gccaattgac tcttgaatgg tttcagcact ttggactgta gaactcaacg
      541 actcaaaaac aggcactcac gttgggctga gacacaagca cacattcctc tgcacgcttt
      601 ttcgatgtca cttatcctta gagcgaggca ccaccacttt cgtaataccg gattcgcttt
      661 ccggcagtgc gcccagaaag caagtttctc ccatccttct caacttaaag actaagactg
      721 tcatgaaaaa gaccaaaatt gtttgcacca tcggaccgaa aaccgaatct gaagagatgt
      781 tagctaaaat gctggacgct ggcatgaacg ttatgcgtct gaacttctct catggtgact
      841 atgcagaaca cggtcagcgc attcagaatc tgcgcaacgt gatgagcaaa actggtaaaa
      901 ccgccgctat cctgcttgat accaaaggtc cggaaatccg caccatgaaa ctggaaggcg
      961 gtaacgacgt ttctctgaaa gctggtcaga cctttacttt caccactgat aaatctgtta
     1021 tcggcaacag cgaaatggtt gcggtaacgt atgaaggttt cactactgac ctgtcagttg
     1081 gcaacaccgt actggttgac gatggtctga tcggtatgga agttaccgcc attgaaggta
     1141 acaaagttat ctgtaaagtg ctgaacaacg gtgacctggg cgaaaacaaa ggtgtgaacc
     1201 tgcctggcgt ttccattgct ctgccagcac tggctgaaaa agacaaacag gacctgatct
     1261 ttggttgcga acaaggcgta gactttgttg ctgcttcctt tattcgtaag cgttctgacg
     1321 ttatcgaaat ccgtgagcac ctgaaagcgc acggcggcga aaacatccac atcatctcca
     1381 aaatcgaaaa ccaggaaggc ctcaacaact tcgacgaaat cctcgaagcc tctgacggca
     1441 tcatggttgc gcgtggcgac ctgggtgtag aaatcccggt agaagaagtt atcttcgccc
     1501 agaagatgat gatcgaaaaa tgtatccgtg cacgtaaagt cgttatcact gcgacccaga
     1561 tgctggattc catgatcaaa aacccacgcc cgactcgcgc agaagccggt gacgttgcaa
     1621 acgccatcct cgacggtact gatgcagtga tgctgtctgg tgaatccgca aaaggtaaat
     1681 acccgctgga agcggtttct atcatggcga ccatctgcga acgtaccgac cgcgtgatga
     1741 acagccgtct cgagttcaac aatgacaacc gtaaactgcg cattaccgaa gcggtatgcc
     1801 gtggtgccgt tgaaactgct gaaaaactgg atgctccgct gatcgtggtt gctactcagg
     1861 gcggtaaatc tgctcgcgca gtacgtaaat acttcccgga tgccaccatc ctggcactga
     1921 ccaccaacga aaaaacggct catcagttgg tactgagcaa aggcgttgtg ccgcagctgg
     1981 ttaaagagat cacttctact gatgatttct accgtctggg taaagaactg gctctgcaga
     2041 gcggtctggc acacaaaggt gacgttgtag ttatggtttc tggtgcactg gtaccgagcg
     2101 gcactactaa caccgcatct gttcacgtcc tgtaatattg cttttgtgaa ttaatttgta
     2161 tatcgaagcg ccctgatggg cgcttttttt atttaatcga taaccagaag caataaaaaa
     2221 tcaaatcgga tttcactata taatctcact ttatctaaga tgaatccgat ggaagcatcc
     2281 tgttttctct caattttttt atctaaaacc cagcgttcga tgcttctttg agcgaacgat
     2341 caaaaataag tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac
     2401 ttgtaacgct acatggagat taactcaatc tagagggtat taataatgaa agctactaaa
     2461 ctggtactgg gcgcggtaat cctgggttct actctgctgg caggttgctc cagcaacgct
     2521 aaaatcgatc agctgtcttc tgacgttcag actctgaacg ctaaagttga ccagctgagc
     2581 aacgacgtga acgcaatgcg ttccgacgtt caggctgcta aagatgacgc agctcgtgct
     2641 aaccagcgtc tggacaacat ggctactaaa taccgcaagt aatagtacct gtgaagtgaa
     2701 aaatggcgca cattgtgcgc catttttttt gtctgctatt taccactact gcctcgcgcg
     2761 taacatattc ccttgctctg gttcaccatt ctgcgctgac tctactgaag gcgcattgct
     2821 ggctgcggga gttgctccac tgctcaccga aaccggatac cctgcccgac gatacaacgc
     2881 tttatcgact aacttctgat ctacagcctt attgtcttta aattgcgtaa agcctgctgg
     2941 cagtgtgtat ggcattgtct gaacgttctg ctgttcttct gccgatagtg gtcgatgtac
     3001 ttcaacataa cgcatcccgt taggctccac ggaatatttc accggttcgt tgatcacttt
     3061 caccggcgtt cccgtccgca cgctggagaa taaggcttta atatccggtg cattcatgcg
     3121 aatacagccc gaactgacgc gtaaaccgac gctgtccggc gcactggtac catgaatgag
     3181 gtattcgcca ttaccatgcg cgaagcgcag tgcgtaacgt cctagcgggt tatttggtcc
     3241 ggcaggaacg actggcagta atttaatgcc acgctccagc gaacgttgac gaatgcctgc
     3301 cgtaggcgtc caggttggat tagggatttt ctgccccaca cgcgtttcca tcaccggcgt
     3361 ttccagcccc tgcaagccaa tacctatcgg ataaacctgc acgatatttt ctcccggcgg
     3421 ataataataa aggcgcagct ctgcaaggtt aacgataatc ccctgacgcg gtgcatcagg
     3481 taataacagt tgtgaaggaa tagttatcgt cgtaccaggt tttggcaccg gggcgatagt
     3541 gttattggct tcaaggatca acattgccgc agtatcaaaa cgtcgggcaa tagcctgaag
     3601 gtttttatcc ccttcttgca ccgtatacgt ttgattttgc ccaaccagtc ggcttccggt
     3661 tggtggtagc ggataatcaa ccgcccaggc agcctggatg gcgctaaaag cgccgataag
     3721 cgtgagtgta agcaaagacg cgcgtttcat tgtaaacctc ctgtatttgc cggagactca
     3781 cgctgaaacg tcggatggcg cttatgttca cctgaaacca aaacactcct gtgcaggtca
     3841 gtgtaaacat tgaccatccg gcaatgtgag ccaaccggat gaaagctgtc cctttagttt
     3901 agctaagtgc agcggctttg gcgcgaattg cgcgaatcat cgcttccaga ccttgtgaac
     3961 gagatggggt gagatgttgg gtgagcgcca ttttttcaaa ccacggacgc acatcgaaat
     4021 tgacaatatc ctgcggcgtc atctgatcgt agagaataaa tacgaccgca ataagccctt
     4081 tcacaatcgc cgcatcgctg tcgccctgca attcaataat tccctgcgca ttctggcgca
     4141 tgacaatcca cacctgattc tggcagccct gaatgctatt ttgtgggctt cggtcttcgt
     4201 cgcgtaattc tggcagacgc tggcccagct caataatgta gagatatttc tcttcccagt
     4261 tggcgcagcg taaaaaatta cgcagcaatt tttctttatc cggcaataaa gccatagtgc
     4321 ctccctgtta tcccagcaga cggtgaatac gttgcaggcc ggtcaccaga cgatccactt
     4381 cttcatgagt gttatacatg gccagcgacg cccgacacat cgcagggacg ttgtaatagg
     4441 ccattaatgg catagcgcag tgatgtccgg tacgcacagc aatgccgtaa ttatcgagaa
     4501 aactgccaac atcataggcg tggtgtttac cgagattaaa agcaataacg ccaagcctgt
     4561 tttgtgggcc atagagagtg agattcggta cagattccag ctgtgatagc gcgtaatgca
     4621 tcagattcag ttcatactcg gctatgttat taagccccag caccgaaaca tattccagcg
     4681 ccgcgccaag accaatgatg cccccggtat tgggtgtacc ggcttcaaac cgccatggtg
     4741 ctttggtcca ggtagtgcct tcactcaggc tgacggtggc gatcatagaa ccgccccctt
     4801 cccacggcgg catctcctgc aacaaggctt ctttgacata aagaatgcca atcccggtgg
     4861 ggccatacag tttatgcccg gaaaacacgt aaaaatcgca atccagcgcc tgaacatcca
     4921 ccggatgatg catcaccgcc tgagcgccat ccaccagcac ttttgcgcca tgctggtgcg
     4981 caagcgtgat cattcccgcc agtggatttt ctgtgccaag cacgttggag acatgagtaa
     5041 ttgccagcag gcgagttttc tcatcaaaca gcgtatgcag cgtctccagt tgcagcgtac
     5101 cgtcggggtt gagagggatc acacgcagct ctgcgccaac gcgtgcgcaa agcatctgcc
     5161 aggggacaat gttagcgtgg tgctccatct gactgatgat gatgttatcg cccgcccgca
     5221 cattgctgtt gccccagcta ttggcgacca gattgatccc ttccgtcgtg ccgcggacga
     5281 acaccagctc ttccgccgaa cgggcattaa taaacagcga tgcccgcttg cgcacgttct
     5341 ccattttctc ggtcgcctgg gcgcttaagg tatgaatacc gcgatgcacc gccgcgtagc
     5401 catgacgata aaactcggcc tcggcgtcaa tcacctggct cggtttctgt gcactggcgg
     5461 cgctgtcgag ataagccagc ggcaaaccgt ttacctcacg cgaaagcacc ggaaagtcgg
     5521 cccgcacttt gtcgacggaa aaagtcatct tgcacctcct ggcagccgtt gaccgattcg
     5581 ggccagcacc tgctgtttaa gcccctcatc acgcagtgct tccgtcagtt cggcagcaaa
     5641 ggcgtaaatg atcatctgct gggcatcctg ctgattgatc ccgcgcgagc gcagatagaa
     5701 catctgttca tcatcaatac gccccaccgt cgcgccgtgg ctgcatttca catcatctgc
     5761 atagatttcc agctgcggtt tcgtatccac ttccgccagt ttgcccatca gcagattatt
     5821 gttggtcatc tgaccatccg ttttgatggc gtgctgcgcg acgttgatca aaccgttaaa
     5881 taccgcgcgg cctttgtcgc tgacgatagt tttgtgcaac tgtcggctgt tacaaaaacc
     5941 tttattgtgt tccagccagg tacgggtatc acacacctcg tttttcaccg gcatcgccag
     6001 gctattgatc cgcagcgtgc tgttttcgcc attgagttgc gtactggtgt tgtgtcgtaa
     6061 cactgcgcca cccagcagga aactatggct aaatgcggtg gcatcctcag ccagcaacaa
     6121 atcgttatga gcaaagtggt gactgagcgg gttttcaaac gccagcttga tatgctgcaa
     6181 gtgggcgttc gctgcgacat tgatagtgaa ccgtgccccg gtaaaatgac gagcatcatt
     6241 aaggctgaca aaatgttcga tcaccgttgc ttcggcacct tccgccagat ccagatgatg
     6301 ccggtaatgg gcagtgttca cctcttcacc tgccacgccc tgggtgatat acattaacag
     6361 caatggcttt gccggccgtt gaccgcgctt cacggcgata tgcgtcacgc tttgtgccag
     6421 gctttccgtc aaatgcagaa acacttccgc ctgaatagcg tcgggtaacc cctgacggtc
     6481 gtcgttaatg ctcacttcat atccgctgcc ttcaattgca tcgctcagcg cgggcacgta
     6541 acgtccatcg acaaacacca gccgcacggc gtctaacgtt aacgctaagg catcacgctg
     6601 ctgtggggat atctcccccg caatgctgac aaactggcta ttggtcagcc cttccagcga
     6661 cgtatatttc cagttttcat gtttacgtgt cggcagtccg gtacgcagca attgttgtaa
     6721 atgctgctgt gcttgcgggg agcgttttgt cccttcagct tcaaacaagt gatgccactg
     6781 ttgcagcgcg ttactgctgt tcggtaagcc agccataacc ctgctcctcc agttgtttga
     6841 ccaacgtgaa atcgccggat ttcacaattc gtccctgata tagcacatgg acgtaatcag
     6901 gcttgatgta gtcgagaatg cgttggtagt gcgtaacaat gatgaatgag cgcttgccat
     6961 cacgcaggga gttcacgcca tcggcgacca cttttaatgc gtcaatatcc agcccggagt
     7021 ccgactcatc aagaatgcat aactccggtt ccagcaccgc catttgcaaa atatcgttgc
     7081 gctttttctc gccgccggaa aaaccaacgt ttaccgaacg ggttaataaa tcttccggca
     7141 tcttcaggag agcgattttc tcttccatca aatcctgaaa atcaaagcgg tcgagcgttt
     7201 cctggccgcg atagctgcgc actgcattaa gtgccgtttg caggaaaaac tggttactga
     7261 cgcctggaat ctccaccgga tactggaagg ccataaagat gccttcgcct gcacgatctt
     7321 ccggcgacag tgcaagcaaa tctttgcctt tgaactcaac cgtgccgccc gtcacttcat
     7381 aatcttcgcg cccggcaagc gttgccgata aggtactttt gcccgaaccg tttggcccca
     7441 taatggcgtg aacttcgccg ggacgaacgg cgaggcttaa tccacgcagg atagctttat
     7501 cttccacgct gacgtgtaaa tctttaatac ttaacatgtt tattccttat ccgacgctgt
     7561 gttcaagact gatcgcgagg agtttttgtg cttcaacggc aaattccagc ggcagttctg
     7621 agaacacgtc tttgcagaaa ccgttaacaa tcatcgagat ggcgtcttct tcgctgatcc
     7681 cgcgttgcag gcagtaaaac agttgatctt caccaatacg tgatgtcgtt gcctcgtgct
     7741 ccagttgtgc gctgttattg cgacattcga catacgggaa ggtatgcgcc ccacaattag
     7801 cgccaatcag cattgagtcg cactgagtga aattgcgcgc attggttgcc gtcggcatga
     7861 ttttcactaa gccgcgataa ctgttctgac tatgtccggc agagatccct ttcgagataa
     7921 tggtcgattt ggtgttttta ccgatgtgga tcatcttggt gccggtatcc gcttgctgat
     7981 gaccgctggt cagcgccact gagtaaaact caccaatgga gttatcgccg cgcaaaatgc
     8041 agctgggata tttccacgta atcgcggacc cggtttctga ttgcgtccat gacattttgc
     8101 tgttttcgcc ttcgcacaaa gcacgcttgg tgacgaagtt gagaataccg ccggtgttgt
     8161 tatcgccagg gaaccagttt tgtaccgtgg aatatttcac ctcggcgttt ttatggatga
     8221 tgacttccac cactgccgcg tgtaactgat agctgtcacg caccggagcg gaacagcctt
     8281 caatgtagct gacgtagctg tcttcgtcgg ccaccagaat ggtgcgctca aactgcccgg
     8341 ttttttccgc gttaatgcga aaataggtgg aaagttccat cgggcagcgc acgcctttag
     8401 gcacataaat aaacgtaccg tcagaggcta ccgccgcatt aagcgcggca aagaagttgt
     8461 cattccccgg caccacggtg ccgagatatt tacgcaccag ttccgggtga tcgtggatcg
     8521 cctcaccaaa ggaacagaaa ataattccct gctccgccag tttttcgcga taagtagtgg
     8581 caaccgaaac agagtcgaaa atggcatcca ccgccacctc tttgccttcc cgcacgggaa
     8641 cgcccaactg ctcaaacgcc gcctccacct ctttacttaa aaaggcgttc gcgccagttt
     8701 gctgcaccgc gccaggttca gacgcgcaag tgtcgtcaca attaccgcac gatggtgctg
     8761 agtagtagct gtaatcctga taattcagct tgtcgtagtg cgctttcaac cagtgcggtt
     8821 cttccatctc cagccatgcg cgataggcgt ttagacgaaa ctccagcatc cactccggct
     8881 cattacgctt cgccgaaatt gcgcgcacca cctcttcgtt tatccccttt gccagctcat
     8941 cggtggctaa ctgggtgaag aatccttctt tataattcag cgggccgccg gtccaggttt
     9001 tgacatcgtc agttgcttca gtattacgag acatagtacc gcctataccc caaagctttc
     9061 gccacagcca cattcattct gggctttagg gttgtgaaat ttgaatatct gattaagtcc
     9121 ttcacgaacg aaatcgactt ccgtgccatc aataaacggc atcgcttgca gcgggacaaa
     9181 cagcttcgcg ccgtcgtgtt caaacagcag atcgtctttg tccggctcgc taacactgtc
     9241 gagcacatag ccaaagcccg cgcagcccgt ttgcttcacg cctaaacgca cgccgaccat
     9301 acccggctgc tttgccacca gctcacggat gtgtatcgcc gctgcgggtg tcagcgttaa
     9361 gccttgccag gcgaaatctt gtgggttaaa ggttcctgaa tgcatgtcca tcgatttacc
     9421 tcacttcatc gctttcagcg tataacagca tgttggtgat aatgattatc agttcaaccc
     9481 agcaaacgca ggggctttac cgtaaaacat gctttttgcc tgcttttaat aagcatagac
     9541 cctgatgtgt gggttaacag gcacgctaag aattagcgta tctcattgtt agataatggt
     9601 tattatctaa ggagcattaa ggctgtgatg aaaaagaaaa agttgtatta aaatgactat
     9661 ttaagagata ggtaaaaaag tgcagcgttc agaaataaga aaacccttaa gtctgtgcga
     9721 cacaggctta agggtttcta ccccatccgg cgcttatctc cggcactctc agtggcttag
     9781 ctcttgaagg ggcgataaga ataatctcat aaagctaacc cgccgtttta acacaaactg
     9841 cgattagtat tatttttgaa caatatcagg cggtagataa gcagtattaa gaaggtcatc
     9901 gaacctggac ggaggttaat ccaggtcgat ttggcgaact tgcggcatta agtcaggatc
     9961 aatgcacgcc cagacgccag gcaaagtaga tttcttcttt taattcagca gaagagagag
    10021 taagcaggtc agcaaattca agttctagtt gtttcagacg tttgagatat tgggcaggtg
    10081 aaagattgct ctggtcacgg cgtaaaaatt caatggccag ctgggtggga tcaagttgag
    10141 tagacatagc atcctcgctt ttagacaaga cgtgtacagt ataccaccgt ttactgtgca
    10201 gataatgacc aaaagcaata tgcgtcacac ttttctggtg acaacgtcac aaaatggcgg
    10261 tcgtcaatcg tgacgaacag cacaaacgcc ctttctcatc gaagatttca atctgccaga
    10321 cctggtgacg cgaaccgaga tgcaacggtt tacatatgcc gcgcacccgc ccttctcgtg
    10381 ccgagcggac gtgattagca ttgatttcca gaccaaccac tttttgctca ccttcggtac
    10441 ataaataacc ggcaacggaa ccgatacttt cggccagtac cacggatgct cctccatgca
    10501 gcaacccgaa aggctgcttt gtccgcgagt ctactggcat tgtcgcttca agggtgtcat
    10561 caccaatatg ttcaaagcga atatccagga accccaccat gtttccttca cccatagcat
    10621 tcagtgcttc cagggtgatt ttccgtttcc atatcattta ataatctcca gtaaagcctg
    10681 cacaggatgg cgtacccccg tgccttcaac ccgttttacc tggctacggc aggaatatcc
    10741 ggtcgccaga cagcggttac gcggcagtcg ctgcatagcc tgatgccagg ataactcata
    10801 gatcccgagc gaattttcat ggtttttcgc ttcatgtccg taagtccctg ccatgccgca
    10861 gcaacccacg ctgacatttt ccagtttcgc gccaaaacgg gcaaatatcg cggcccattg
    10921 tgctggcgca cccggcaagg cggtaacttc ggtacagtga ccaaagaaat accatgattc
    10981 accgctgact gtagccacag gctgtgactc aagtgcgctt gccagccatt cattcgccag
    11041 taagacgtta aactcgccac gctcctcgcc cagcgccagt ttatattcat cgcgataaca
    11101 aagtaccagc gccggatcga cgcccaccat tggcataccc agcttcgcca tacggttgag
    11161 gaaatccgcc gtctttttcg ccgtcttcgc aaaacgatta agaaaacctt taatatgctg
    11221 ggctttgcca tttggcgaaa atggcagtaa cacaggctgg aaacctaatt tttcgaccag
    11281 acggacaaaa tccgccacca cttgcgcatc gtaatagctg gtaaaggggt cctgcaccac
    11341 caacactgtg cgcgctttct gctctgcatt gagagattca agctgttcca gcgtcatgtt
    11401 tgccgagcga tgccccacca tttgttgttg tagcgagggg accgacagca gcggcaaatc
    11461 aaccatgccg atatgttttt ccgagagttt gcgcaccagc ggctggttaa tgaagaagtt
    11521 aaaggttttc ggcgcgcgtg ccatcagcgg cgcgtagctc tcgaccgtag cgacgaggtg
    11581 gtcgcgcagc gggcgtaaat aacgggtgtg atagagctgc agaaaacgag agcgaaactc
    11641 cggcacatca attttgatgg ggcacagggt cgaacacgct ttacaggcca gacagcccga
    11701 catcgcctct ttgacttcgt gtgagaagtc atattcgcct ttattcgcat gccagctatt
    11761 gcgcgtgcgg gcaattaacg tccgcaaact gacgccggat tcaggcagtt cttgttccag
    11821 tttgagtgga tcaacgccgc gatccgccaa caaacgcagc cattcacgca ccagcgttgc
    11881 gcgccctttc ggtgaatgaa tccggttctg ggtgatcttc atcgacggac acatcggact
    11941 acgggcatca aagttgaagc ataaaccgtt gccgttacac tccatcgcac cgcgccactg
    12001 ctggcgtacc gcaatgggga tctgccgatc gaatgtaccg cgcttcaccg cgtccacttt
    12061 catcatcggc gcatcgagac cttctggcgg gcaaatcttc cctgggttga gtcggttatg
    12121 cgggtcaaat gccgctttca ctttgcgcag ttctgcaaaa agttcctcac cgaaaaacgc
    12181 cgggctgtat tcagcgcgaa aacctttgcc gtgctcgccc cacaacaaac caccgtattt
    12241 cgcagtcagc gccaccacgt catcagagat ttgcttcatc aaaatctctt gttgaggatc
    12301 gcacatatcc agctctggac ggacgtgcaa gacacctgcg tcgacgtgac cgaacatacc
    12361 gtagcttaag ccgtggctgt cgagcagcgc gcgaaattca gcaatataat ccgccaggtg
    12421 ttccggcggt acgcaggtat cctcagcaaa cggaattggc ttagcggcac ctttggcatt
    12481 gccaagcaga ccaacggctt ttttgcgcat tgcatagata cgttcaaccc ccgccagctc
    12541 acggcacacc tgccagccga tgacacctgc ttgatgactg gcgatcagct catccaaccg
    12601 cgcacagagt gcatttaccc gctcatcaat cagcgcctca tcatcaccag caaattccac
    12661 aatgttcagc ccgagcatct cttggtcagg cacatcggta atcaactcgc tgacggaatg
    12721 ccagacaata tcttcccgcg ccagattcag cacttttgag tccaccgtct ctaccgaaag
    12781 cgcccgcgcc tcaaccataa acggcgcgtt acgcagcgca gagtcaaaag agtcatattt
    12841 gacgttcacc agacggcgca ctttaggcaa gcgcgtaata tccagccgcg cttcggtaat
    12901 aaaggccagc gtcccttctg aacccgtcag aatgcgcgtc aggtcgaact cggtcatctc
    12961 atcgttaaag acatgacgca gatcgtaacc ggtaagaaag cggttaagtt tggggaagtt
    13021 gtcgataatt aactggcgtt gctgacggca acgttgataa accgtgttat aaattcgccc
    13081 gattgtggta ttggatttac ccagcgtttc cgccaattcg acgggtaaag gttgcgtatc
    13141 gagaacatcg ccccccaaca acaccgcgcg tacgccaagt acgtgatctg acgttttgcc
    13201 atagaccagc gatccctgac cggatgcatc ggtattgatc atcccaccga gcgttgcccg
    13261 gttgctggtc gaaagttccg gcgcaaaaaa gtagccgaac ggtttcaggt actgattgag
    13321 ttgatctttt atcaccccgg cctcaacgcg cacccagccc tcttcagggt taatttcgat
    13381 gatgcggttc atatggcggg acatatcaac aataatcccc tggttgagcg cctgaccgtt
    13441 agtgccggtg ccgccgccgc ggggggtaaa gatcagcgat gaatagcgtt cctgcgcggc
    13501 aagacgggcg atcagcgcca catctgcggt tgaacgcgga aataccaccg catcggggag
    13561 aagttggtaa atactgttgt cggtcgacat tgtcagacga tcggcataac ttgtcgccgt
    13621 atcgccggta aaaccttgtt gctccagctc ttgcaaaaaa ttaagcacca gttgaacgac
    13681 gccgggtgcc tgagaaatct gtggaatcat tatattgccc ctttcctgcg gtctgtgatg
    13741 taggtcgata cactattctt tcaggcggct gcaatagcgc actgaaaggt gatgtttatt
    13801 tactctatgg atttcgagtt gcaggaaggc ggcaagcgag tgaactccag gagcttacaa
    13861 tagtaagtga ctggggtgaa cgaacgtagc cgcagcacat gcaacttgaa atacgacgag
    13921 taaatcgttt gcgtgttgcc tgagttgttg taccacattt ttttctaaca cgcccatcag
    13981 aattaagggc agaatcggcc tgttaaaaac cgctgaaatt gctcatcatt atgcaggtga
    14041 gtttcgcgtg ttcacgtcgc gtcgacgatt tgacgcacaa aaaaggtgaa aagtagttat
    14101 ggtaaatgtt cgtcagccca gggatgtcgc acaaattctg ctttcggtgc tgtttttagc
    14161 catcatgatt gtggcatgtc tgtggattgt tcaacccttt attctcggct ttgcatgggc
    14221 cggtacggtg gttatcgcca cctggccggt attgttacgt ttgcaaaaga tcatgtttgg
    14281 ccgccgctct ctcgccgttc tggtgatgac gctgttatta gtgatggtgt ttatcatccc
    14341 tattgctttg ctggttaaca gtatcgtcga cggcagcggc ccgctaatta aagccatttc
    14401 cagcggcgac atgacgttac ccgatctggc gtggcttaat accattccgg tgattggcgc
    14461 gaagctgtat gcaggctggc acaacttgct ggatatgggg gggacggcga tcatggcgaa
    14521 agtccgccct tatattggca ccaccaccac ctggttcgtt gggcaggcgg cgcatatcgg
    14581 gcgctttatg gtgcattgtg cgctgatgct tctcttcagt gccctgctgt actggcgcgg
    14641 tgaacaggtg gcacaaggca ttcgccattt tgcaacccgt ctggcaggcg ttcgcggaga
    14701 tgccgccgta ctgctggcgg cacaggctat ccgcgcggtg gcgctgggtg tggtggtgac
    14761 ggcgttagta caggcagtgc ttggcggtat cggcctcgcc gtatccggcg taccttatgc
    14821 aacgttgcta acggtgttaa tgatcctctc ctgccttgtc cagcttggcc cgttgccggt
    14881 actgattccg gcgattatct ggctctactg gactggcgat accacctggg gaacggtatt
    14941 gttagtgtgg agcggtgtgg ttggcacgct ggataacgtc atccgcccaa tgttaattcg
    15001 catgggtgcc gatttaccgc tgatcctgat cctctctggt gttattggtg gtttgattgc
    15061 tttcgggatg atcggtctgt ttattggtcc ggttctgtta gccgtttcct ggcgtctgtt
    15121 tgccgcgtgg gtggaagaag tcccgccgcc gacggaccaa ccggaagaaa ttctcgaaga
    15181 acttggcgaa atcgagaaac cgaataagta atttctcatc aggcggctct gccgcctgat
    15241 tgttaacccc cgctaattga tatttgcttg ctcttcccca tattttcctg cttaccgcca
    15301 ttcagctggt agtacctgtc gcaaattctt tacagttttt aaactaatga gacgaatctg
    15361 atcgacgcaa aaagtccgta tgcctactat tagctcacgg ttataaatca acatattgat
    15421 ttataagcat ggaaatcccc tgagtgaaac aacgaattgc tgtgtgtagt ctttgcccat
    15481 ctcccacgat gggctttttt ttaacatttt tccacttcgc tacctcgccc ctcactcttt
    15541 ccaataatgt tgcacaagat actgtgactc tcttcaagcc gtcagcaggc tgataatgtt
    15601 atttatattt tattgtttat ttcatgcagc cattacacga tgaacgctta tgaactccag
    15661 gcactccgac atatttttgc catgactatt gatgaatgcg cacctggatt gcccagaccg
    15721 gtgacagtga aagctggcgt cagtgggaaa atggcaagtg tgccattcct gatcgtgtag
    15781 tcgagcaact gttggctatg cgtcagcaaa gaaaaaaaca tcttcatgcc atcatagaga
    15841 aaataaataa ccgcatcggc aacaacacca tgcgtttttt ccccgactta accgcatttc
    15901 aacgagtcta ccctgatggc aattttattg actggaagat ctatcaatcg gtagccgctg
    15961 aaggtaatgc tgttgaaaag caaattcccc agccagttgg tggacgccag cgtagctaat
    16021 gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc atcggtactg
    16081 ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc caaatatgcg
    16141 atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta ttacagtatg
    16201 ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt ccggcccatc
    16261 tgggctaatg tatttaactc tgcactggca acaataacag cagccattat ctatctgtac
    16321 ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc agctggcggc
    16381 attttacagc tcggtgtttc ggtcatgtca gagtttttcc caaaaagcaa agccaaagtc
    16441 accagtattt atatgatgat gggtggactg gctaactttg ttattccgct gattaccggt
    16501 tatctgtcga acatcggcct tcaatatatc attgttctcg attttacttt cgcgctgctg
    16561 gccctgatta ccgcaattat tgtttttatc cgctattacc gcgtttttat tattcctgag
    16621 aatgatgtgc ggtttggcga gcgtaaattt agcacccggt taaacacaat taagcataga
    16681 ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg ctgatggcct
    16741 atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa aaagcgggat
    16801 tgccatttac ctatatggcc tttgaagtgg ataacgatag ttttcccgga gcaattgaag
    16861 gattaaaagc tctaaaaatg cgcggaactg gtgtatcaat gccgaacaaa caactggctt
    16921 gttaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc aacaccatcg
    16981 ttaatgatga tggctacctg cgcggctata acaccgacgg cacgggccat attcgcgcca
    17041 ttaaagagag cggttttgat atcaaaggca aaacaatggt gctgttaggg gccggaggtg
    17101 cctcaacggc tattggcgcg cagggggcaa ttgaaggttt aaaagaaatt aaactcttta
    17161 accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt aacgaaaaca
    17221 ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct gaagccctgg
    17281 cttccgccga cattttaacc aatggcacaa aagtgggtat gaaacccctt gagaatgaat
    17341 cattagttaa tgatatcagt ctgttacatc cgggacttct ggtcactgaa tgcgtgtata
    17401 acccgcatat gacgaagtta ttgcagcagg cgcaacaagc tggttgcaaa acgattgatg
    17461 gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact ggcaaagatt
    17521 tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct gaccgcgtgc
    17581 agaaagggta aaaaaatgaa aaccgtaact gtaaaagatc tcgtcattgg tacgggcgca
    17641 cctaaaatca tcgtctcgct gatggcgaaa gatatcgccc gcgtgaaatc cgaagctctc
    17701 gcctatcgtg aagcggactt tgatattctg gaatggcgtg tggaccactt tgccgacctc
    17761 tccaatgtgg agtctgtcat ggcggcggca aaaattctcc gcgaaaccat gccagaaaaa
    17821 ccgctgctgt ttaccttccg cagtgccaaa gaaggcggcg agcaggcgat ttccaccgag
    17881 gcttatattg ctctcaatcg tgcagccatc gacagcggcc tggttgatat gatagatctg
    17941 gagttattta ccggcgatga tcaggttaaa gaaaccgtcg cctgcgccca cgcgcatgat
    18001 gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc cggaagccga agaaatcatt
    18061 gcccgtctgc gcaaaatgca gtccttcgac gccgatattc ctaagattgc gctgatgccg
    18121 caaagtacca gcgatgtgct gacgttgctt gccgcgaccc tggagatgca ggagcagtat
    18181 gccgatcgtc caatcatcac aatgtcaatg gcaaaaactg gcgtaatttc tcgtctggct
    18241 ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa aaaaagcctc tgcgccaggg
    18301 caaatctcgg taaatgattt acgcacagta ttaactattt tacatcaggc ataagcaata
    18361 atatttcggc gggaatatcc tcccccgccg aactaaaaaa aatatattca accgtattta
    18421 ataaaaatat ttcgtgagtc tctgtgcgct aaatatccat ctggcgtagg gaaaatcaca
    18481 cctgaatcag aaattaacaa tgaaacctgt aaaaccacct cgtattaatg gacgagtgcc
    18541 ggtcctgtcg gcacaggaag cggtgaatta tattcccgac gaagcaacac tttgtgtgtt
    18601 aggcgctggc ggcggtattc tggaagccac gacgttaatt actgctcttg ctgataaata
    18661 taaacagact caaacgccac gtaatttatc gattattagt ccaacagggc ttggcgatcg
    18721 cgccgaccgt ggtattagtc cgctggcgca agaaggtctg gtgaaatggg cattatgtgg
    18781 tcactgggga caatcgccgc gtatttctga actcgcagaa caaaataaaa ttattgctta
    18841 taactatcca caaggtgtac ttacacaaac cttacgcgcc gccgcagccc accagcctgg
    18901 tattattagt gatattggca tcgggacatt tgtcgatcca cgccagcaag gcggcaaact
    18961 gaatgaagtc acgaaagaag acctgattaa actggtcgag tttgataaca aagaatatct
    19021 ctattacaaa gcgattgcgc cagatatcgc cttcattcgc gctaccacct gcgacagtga
    19081 aggctacgcc acttttgaag atgaggtgat gtatctcgac gcattggtta ttgcccaggc
    19141 ggtgcacaat aacggcggta ttgtgatgat gcaggtgcag aaaatggtta agaaagccac
    19201 gctgcatcct aaatctgtcc gtattccggg ttatctggtg gatattgtgg tggtcgatcc
    19261 ggatcaaagc caactgtatg gcggtgcgcc ggttaaccgc tttatttctg gtgacttcac
    19321 ccttgatgac agcaccaaac ttagcctgcc cctaaaccaa cgtaaattag ttgcgaggcg
    19381 tgcattattc gaaatgcgta aaggcgcggt ggggaatgtc ggcgtcggta ttgctgacgg
    19441 tattggcctg gtcgcccggg aagaaggttg tgctgatgac tttattctga cggtagaaac
    19501 aggtccgatt ggcggaatta cttcacaggg gatcgccttt ggcgcgaacg tgaatacccg
    19561 tgccattctg gatatgacgt cccagtttaa tttttatcac ggtggtggtc tggatgtttg
    19621 ttatttgagt tttgctgaag tcgaccagca cggtaacgtc ggcgtgcata aattcaatgg
    19681 caaaatcatg ggtaccggtg gatttattga tatcagtgcc acctcgaaga aaattatttt
    19741 ctgcggcacg ttaaccgcgg gcagtttaaa aacagaaatt gccgatggca aattaaatat
    19801 cgtccaggaa ggacgggtga agaaatttat tcaggaacta ccggaaatta cttttagcgg
    19861 aaaaatcgcg ctcgaacggg aactggatgt tcgttacatc accgaacgtg ctgtattcac
    19921 gctgaaagaa gacggcctgc atttaatcga aatcgcccct ggcgtcgatt tacaaaaaga
    19981 tattctcgac aaaatggatt tcaccccagt gatttcgcca gaactcaaac tgatggacga
    20041 aagattattt atcgatgcgg cgatgggttt tgtcctgcct gaagcggctc attaacagga
    20101 gtatcacgat ggatttttct ttaactgaag aacaagaact gctgctggcc agtattcgcg
    20161 aactgattac gactaacttt gcggaagagt atttccgcac ctgcgatcaa aacgggacat
    20221 atccgcgtga gtttatgcgg gcgctggcgg ataacggcat ttctatgctt ggcgtgccgg
    20281 aagagttcgg tggcatccct gcggattacg tcacccagat gctggcgctg atggaagtgt
    20341 caaaatgcgg tgctccggca tttttgatta ccaacggtca atgtattcac agtatgcgcc
    20401 gtttcggttc tgcagagcag ctacgtaaaa cggcagagag caccctggaa acgggtgatc
    20461 ccgcctatgc cctggcgttg acggagccag gcgcaggctc agacaacaac agtgccacta
    20521 ccacttacac gcgtaaaaac ggcaaggttt acatcaacgg acagaaaacc tttattaccg
    20581 gtgcgaaaga gtacccgtat atgctggtgt tggcgcgcga tccgcaaccg aaagatccca
    20641 aaaaagcctt caccctgtgg tgggtcgact ccagtaagcc cggcattaag attaacccgc
    20701 tgcataaaat cggctggcat atgctcagca cctgcgaagt ctatctcgac aacgtggagg
    20761 ttgaagagag cgacatggtg ggcgaagaag gaatgggttt cctcaatgtg atgtacaact
    20821 ttgagatgga gcgcctgatc aacgccgcgc gcagcaccgg ctttgccgaa tgcgcctttg
    20881 aagatgccgc ccgctatgcc aaccaacgta tcgcttttgg taagcccatt ggtcataacc
    20941 agatgatcca ggaaaaactg gcgctgatgg cgattaagat cgacaacatg cgcaacatgg
    21001 tgctgaaagt ggcatggcaa gccgatcagc atcagtcact gcgcaccagc gcggcgctgg
    21061 caaaactgta ttgcgcacgt accgcaatgg aagtcattga tgatgcgatt caaatcatgg
    21121 gcggtctggg ctataccgat gaagcgcgcg tctcccgctt ctggcgtgat gtccgttgtg
    21181 aacgtatcgg cggcggtaca gacgaaatta tgatttacgt agcaggtcgg cagatcctga
    21241 aagactatca gaacaaataa tctgcaggcg gcgcagcttc ttaacaaact gcgccgccag
    21301 atttatccaa caagacttac cggttgagga aattcagata ggatttcttg ttccgcaaaa
    21361 cagtttttaa attgtctacg gtaatcgctg ggcgaacaac cgacatggcg caaaaacagt
    21421 ttggcaaagt gatcgacatt ttcataaccc acacgccagg agatctctgc ctgtgataat
    21481 tcagtattag tgagtgacca ttttgcttcc gtcatacgcc gttgaataac atagttaata
    21541 ggggaaatac gatactcttt ggtaaattca tggcaaatat aactgacgct ggcacgaaat
    21601 tttttcgata actgttctaa agtgattttt tcgcgataat tattactcag atagaaaaga
    21661 atatctttta tcagaacatc ttttttaata tacccctgct ccgaacgata agcattttta
    21721 aaattttcgt agtaaagaat ggctaacgta taggcaaatg catcccataa cgaagatgtt
    21781 tggctatttt tactttgcgg caaaatcaca cttagctcat taaataaggt ttttatgact
    21841 tcttttccct gccccgcggc aattaccgga caagaatgcg gttgcagtag ctgattttcc
    21901 tcagccccct gaaactgaaa gccgtacagc gcacaggtac acgtcgttgc cggatcgtta
    21961 acgtcagagg ccaccgcatg cagcctgccg cgttctatca ccacaatgtc atccgcatgt
    22021 gcgacatata acgaagaatc gatggtaaac cttgcaaccc ctttcttgac gtaaatcaac
    22081 tcggtttcat tgtcatgaac gtgatgtccg gactcccatt ttggatcatc gctaaatgca
    22141 aaacgtgaaa gccgtggcgt tttaccggcg acaaacagcg tttcactggc gttatcaaaa
    22201 cagcgttgat acatgacaac ctccctattc catgagcaag caaaaacaat atatgccgga
    22261 taaaatacgg cgtgtattgc ggttatacaa ccgcgtttag cgagagctaa acaagattat
    22321 ttacactgaa tgcaagattg tacggtcatg gaataactat attgccgcaa attgcacttt
    22381 tgttttagcg atcgcatttt tttgcaagat tgttggcaag gaaaacagct tgctccgtcg
    22441 aaaaccccca ccgctatcgc acactatttt caggccattt ttaccttcca tcggagatgg
    22501 ttccgtatgc gactcacagg agaaatcatg aaaataataa cctgctttaa gctggtgcct
    22561 gaagaacagg acattgttgt cactccagaa tacaccctga atttcgacaa tgccgacgcc
    22621 aaaatcagcc agttcgatct caatgccatt gaagctgcaa gccagctcgc aacagatgac
    22681 gatgagatag ccgcgctgac cgttggcggc tcattgttgc agaactcgaa agtgcgcagt
    22741 gcgcaaagat gtgctatccc gcgggccgca cagcctgtat ttggtgcagg atgcgcaact
    22801 tgaacatgca ctgcctctcg ataccgcaaa agcgctggcg gcagcaattg aaaagatcgg
    22861 cttcgattta ctgatctttg gtgaaggttc cggcgacctt tatgcccagc aggttggctt
    22921 gctggtcgga gaaattctgc aacttccggt gattaatgca gtgagtgcta ttcagcgtca
    22981 gggtaataca ctggtgattg aacgcacgct tgaagatgat gttgaagtta ttgaactctc
    23041 tgttccagcc gtgctctgcg tcacctccga tattaacgtg ccacgcattc cttcgatgaa
    23101 agccattctc ggcgcgggta aaaaaccggt aaatcagtgg caggcaagtg atattgactg
    23161 gagccagagc gcgccacttg ctgaactggt cggcattcgc gtaccgccgc aaacagaacg
    23221 taagcacatc attatcgata acgattcgcc ggaggccatt gccgagctgg cggaacatct
    23281 gaagaaagcc ctgaactgaa gcctacggag aagaaacgat gagtcaatta aacagcgtct
    23341 gggtctttag cgataatcct gaacgttatg ctgaactgtt tggcggcgct cagcaatggg
    23401 gccaacaggt gtatgccatt gtacaaaata ccgaccaggc gcaggcagtt atgccttatg
    23461 gtccaaaatg tatttatgtt cttgagcaaa acgacgcgct gcaacgcact gaaaattacg
    23521 ccgaaagcat tgctgccccg ctgaaagata aacaccccgc tatgttgctg ttggccgcga
    23581 cgaaacgtgg taaagctctg gcagcacggt taagtgtgca actgaatgcg gcgctggtga
    23641 acgatgccac ggcggtggat attgtcgatg gtcacatttg cgccgaacac cggatgtatg
    23701 gcgggttagc gttcgctcag gaaaagatca acagcccgct ggcgatcatt acccttgcac
    23761 ccggtgttca ggaaccgtgc accagtgata cctctcatca gtgcccgaca gaaacggtac
    23821 cttatgttgc tccgcgtcat gaaattctct gtcgcgaacg ccgtgcgaaa gccgcaagca
    23881 gcgtggacct gagcaaagca aaacgtgtgg ttggcgtcgg tcgtggtctg gcggcgcagg
    23941 atgacctgaa aatggtccac gaactggcgg cggtgctgaa tgctgaagtc ggctgttcac
    24001 gtccaattgc cgaaggcgag aactggatgg agcgtgaacg ttatatcggt gtctccggcg
    24061 tgttgctgaa atccgatctc tacctgacgc tggggatctc cgggcagatc cagcatatgg
    24121 ttggcggcaa cggcgcaaaa gtgattgtcg ccatcaataa agataaaaat gcgccaatct
    24181 tcaattatgc cgactacggt ctggtgggcg atatctacaa agtcgtccct gccctgatta
    24241 gccagttgag ccgctaattc ccctttcaac cacatctccc gctgtaaacg cagcgggaag
    24301 ggagcataac gcatgtcgga tgacaaattt gatgccattg tggtcggtgc ggacgttgct
    24361 agtagcgttg ccgcactggt catggcgcga gccggactgg atgtcctggt gatagaacgc
    24421 ggcgacagtg ccggatgtaa aaacatgacc ggcgggcgtc tttatgccca cacacttgaa
    24481 gcaatcattc caggctttgc agtatcagcg ccggtagaac gcaaggtcac acgcgagaaa
    24541 atctccttct taaccgaaga aagcgccgtt accctcgatt ttcaccgcga gcaaccagat
    24601 gttccgcaac acgcatctta taccgtattg cgtaatcgtc tggacccgtg gttgatggaa
    24661 caagccgagc aggcgggcgc acagtttatc ccgggcgttc gcgtcgatgc gttggttcgt
    24721 gaaggaaaca aggtcactgg cgtgcaggcc ggggatgata ttctccaagc gaatgtggtg
    24781 gttctagctg atggcgtaaa ctcgatgctt ggccgctcgc tgggaatggt tcccgcttcc
    24841 gatccgcatc attacgctgt tggtgttaaa gaggttattg gcctcacacc agaacagatc
    24901 aacgatcgct ttaatattac gggcgaggaa ggtgccgcct ggctgtttgc cggctcccct
    24961 tctgacggcc tgatgggcgg gggatttctc tataccaaca aggattccat atccttgggg
    25021 ctggtttgtg gattgggtga tatcgcccat gcgcaaaaaa gcgtgccgca aatgctggaa
    25081 gattttaaac aacaccccgc cattcgccca ctaattagcg gcggcaaact gcttgaatat
    25141 tccgcgcata tggtgccaga aggcggtctg gcaatggtgc cgcagatggt taacgatggc
    25201 gtgatgatcg ttggtgacgc cgcaggcttc tgcctgaatt tgggttttac agttcgtggc
    25261 atggatttag ccattgcatc ggctcaggct gccgccacaa cggtgatcgc cgccaaagaa
    25321 cgcgaggatt tctccgccag cagtctggcg caatacaaac gtgagctgga acaaagctgc
    25381 gtcatgcgcg atatgcagca tttccgcaag atcccggcgc tgatggaaaa cccgcgcctg
    25441 tttagccaat actcacgaat ggtcgccgac atcatgaacg agatgttcac cattgacggc
    25501 aaaccaaacc agccggtacg caaaatgatc atgggacacg cgaagaaaat tgggctgatc
    25561 aacttgctga aagatggcat taagggagca accgcgctat gagccagaac gctacggtta
    25621 acgtcgacat caaattaggc gtcaataaat tccatgttga tgagggccac ccgcatatca
    25681 ttctggcggc aaatcccgat atcaatgaat tccgtaaatt aatgaaagct tgccctgccg
    25741 gactttataa gcaggatgac gccggcaaca ttcatttcga ttccgccggt tgtctggagt
    25801 gcggcacctg tcgggtgctg tgcggcaaca ctattctcga acagtggcaa tatcccgcag
    25861 gcaccttcgg tattgatttt cgctacggct aatcatgcat cccacaggcc cgcatctcgg
    25921 gcctgatgtt ctgtttcgag agtccaaaat gaaagtgaca ttaacgttca acgaacaacg
    25981 tcgtgcggcg tatcgtcagc aagggttatg gggcgatgct tcactggccg attactggca
    26041 gcagaccgct cgtgcgatgc cagacaaaat tgccgtggtc gacaatcatg gtgcaacgta
    26101 cacctatagc gcgctcgatc acgccgcgag ctgtctggca aactggatgt tagcgaaggg
    26161 tattgaatca ggcgatcgca tcgcatttca actgcctggc tggtgtgaat ttaccgttat
    26221 ctatcttgcc tgcctgaaaa tcggtgcggt ttccgtgccg ctgttgcctt cctggcggga
    26281 agcagaactg gtgtcggtgc tcaataagtg tcaggcaaaa atgttctttg caccgacgtt
    26341 gtttaaacaa acacgtccgg tagatttaat cctgccgctg caaaatcagc ttccacaact
    26401 acaacaaatt gtcggcgtgg acaaactggc tcccgccacc tcttccctct cattaagtcc
    26461 gattatcgcc gacaatacct cactgaccac ggcgataacg acccacggcg atgaattagc
    26521 tgcggtgctg tttacctccg gaaccgaggg tctgccaaag ggcgtgatgc taacgcataa
    26581 caatattctc gccagtgagc gggcttattg cgcgcgactg aatctgacct ggcaggatgt
    26641 ctttatgatg cctgcgccac ttggtcacgc aacgggcttt ctgcatggcg taacggcacc
    26701 attcttaatt ggcgctcgca gcgtgttgtt agatattttc actcctgatg cgtgtctcgc
    26761 gctgcttgag cagcagcgtt gcacctgtat gctaggcgca acgccgtttg tctatgatct
    26821 tttgaatgta ctagagaaac aacccgcgga cctttcagcg ctgcgtttct ttctttgcgg
    26881 cggtaccaca atccccaaaa aagtggcacg tgaatgccag cagcgcggca ttaaattatt
    26941 aagtgtttat ggttccacag aaagttcgcc gcatgcggtg gtgaatctcg atgatccttt
    27001 gtcgcgcttt atgcacaccg atggttacgc tgccgcaggt gtagagatta aagtggtcga
    27061 tgacgcacgc aagaccttac cgccaggttg cgaaggtgaa gaagcctcgc gtggccccaa
    27121 tgtgtttatg gggtattttg atgaacctga attaaccgcc cgtgcgctgg acgaagaagg
    27181 ctggtattgc agcggcgatc tctgccgtat ggatgaggct ggctatataa aaattaccgg
    27241 acgcaaaaaa gatattattg tccgcggcgg cgaaaatatt agcagccgtg aagtggaaga
    27301 tattttattg cagcatccta aaattcacga tgcctgtgtg gttgcaatgc ccgatgaacg
    27361 tttaggtgaa cgatcatgcg cttatgtcgt gctgaaagcg ccgcatcatt cattatcgct
    27421 ggaa
//
LOCUS       DAJVTY010000038        27233 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVTY010000038 DAJVTY010000000
VERSION     DAJVTY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27233
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.038"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            454..3639
                     /gene="rne"
                     /locus_tag="OQQ66_001475"
     CDS             454..3639
                     /gene="rne"
                     /locus_tag="OQQ66_001475"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1904450.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3836..4789)
                     /gene="flgL"
                     /locus_tag="OQQ66_001476"
     CDS             complement(3836..4789)
                     /gene="flgL"
                     /locus_tag="OQQ66_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1904451.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4801..6444)
                     /gene="flgK"
                     /locus_tag="OQQ66_001477"
     CDS             complement(4801..6444)
                     /gene="flgK"
                     /locus_tag="OQQ66_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS1904452.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6510..7451)
                     /gene="flgJ"
                     /locus_tag="OQQ66_001478"
                     /pseudo
     CDS             complement(6510..7451)
                     /gene="flgJ"
                     /locus_tag="OQQ66_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(7451..8548)
                     /gene="flgI"
                     /locus_tag="OQQ66_001479"
     CDS             complement(7451..8548)
                     /gene="flgI"
                     /locus_tag="OQQ66_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1904453.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8560..9258)
                     /gene="flgH"
                     /locus_tag="OQQ66_001480"
     CDS             complement(8560..9258)
                     /gene="flgH"
                     /locus_tag="OQQ66_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1904454.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9311..10093)
                     /gene="flgG"
                     /locus_tag="OQQ66_001481"
     CDS             complement(9311..10093)
                     /gene="flgG"
                     /locus_tag="OQQ66_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1904455.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10231..10986)
                     /gene="flgF"
                     /locus_tag="OQQ66_001482"
     CDS             complement(10231..10986)
                     /gene="flgF"
                     /locus_tag="OQQ66_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS1904456.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(11006..12214)
                     /gene="flgE"
                     /locus_tag="OQQ66_001483"
     CDS             complement(11006..12214)
                     /gene="flgE"
                     /locus_tag="OQQ66_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1904457.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(12239..12934)
                     /gene="flgD"
                     /locus_tag="OQQ66_001484"
     CDS             complement(12239..12934)
                     /gene="flgD"
                     /locus_tag="OQQ66_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1904458.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12946..13350)
                     /gene="flgC"
                     /locus_tag="OQQ66_001485"
     CDS             complement(12946..13350)
                     /gene="flgC"
                     /locus_tag="OQQ66_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS1904459.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13354..13683)
                     /gene="flgB"
                     /locus_tag="OQQ66_001486"
     CDS             complement(13354..13683)
                     /gene="flgB"
                     /locus_tag="OQQ66_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1904460.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            13839..14498
                     /gene="flgA"
                     /locus_tag="OQQ66_001487"
     CDS             13839..14498
                     /gene="flgA"
                     /locus_tag="OQQ66_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1904461.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            14574..14903
                     /gene="flgM"
                     /locus_tag="OQQ66_001488"
     CDS             14574..14903
                     /gene="flgM"
                     /locus_tag="OQQ66_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1904462.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEA
                     QQDLQSN"
     gene            14908..15324
                     /gene="flgN"
                     /locus_tag="OQQ66_001489"
     CDS             14908..15324
                     /gene="flgN"
                     /locus_tag="OQQ66_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1904463.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15364..16899)
                     /gene="murJ"
                     /locus_tag="OQQ66_001490"
     CDS             complement(15364..16899)
                     /gene="murJ"
                     /locus_tag="OQQ66_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1904464.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17066..17932)
                     /locus_tag="OQQ66_001491"
     CDS             complement(17066..17932)
                     /locus_tag="OQQ66_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1904465.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(17934..18581)
                     /locus_tag="OQQ66_001492"
     CDS             complement(17934..18581)
                     /locus_tag="OQQ66_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1904466.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(18592..19176)
                     /gene="rimJ"
                     /locus_tag="OQQ66_001493"
     CDS             complement(18592..19176)
                     /gene="rimJ"
                     /locus_tag="OQQ66_001493"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1904467.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            19412..20614
                     /gene="mdtH"
                     /locus_tag="OQQ66_001494"
     CDS             19412..20614
                     /gene="mdtH"
                     /locus_tag="OQQ66_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1904468.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHRLM
                     AGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSYMG
                     FSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKRAA
                     RRLLERDA"
     gene            20678..21325
                     /gene="grxB"
                     /locus_tag="OQQ66_001495"
     CDS             20678..21325
                     /gene="grxB"
                     /locus_tag="OQQ66_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1904469.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            21459..22019
                     /locus_tag="OQQ66_001496"
     CDS             21459..22019
                     /locus_tag="OQQ66_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1904470.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22125..23171
                     /gene="pyrC"
                     /locus_tag="OQQ66_001497"
     CDS             22125..23171
                     /gene="pyrC"
                     /locus_tag="OQQ66_001497"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1904471.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVMPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23245..23490
                     /gene="dinI"
                     /locus_tag="OQQ66_001498"
     CDS             23245..23490
                     /gene="dinI"
                     /locus_tag="OQQ66_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1904472.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            23780..24034
                     /gene="bssS"
                     /locus_tag="OQQ66_001499"
     CDS             23780..24034
                     /gene="bssS"
                     /locus_tag="OQQ66_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1904473.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24149..25267
                     /gene="solA"
                     /locus_tag="OQQ66_001500"
     CDS             24149..25267
                     /gene="solA"
                     /locus_tag="OQQ66_001500"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1904474.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25315..25428
                     /locus_tag="OQQ66_001501"
     CDS             25315..25428
                     /locus_tag="OQQ66_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1904475.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            25689..26255
                     /locus_tag="OQQ66_001502"
     CDS             25689..26255
                     /locus_tag="OQQ66_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1904476.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26259..26834
                     /locus_tag="OQQ66_001503"
     CDS             26259..26834
                     /locus_tag="OQQ66_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1904477.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFEDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(26876..>27233)
                     /locus_tag="OQQ66_001504"
     CDS             complement(26876..>27233)
                     /locus_tag="OQQ66_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904478.1"
                     /translation="RKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
BASE COUNT         6540 a         7253 c         6871 g         6569 t
ORIGIN      
        1 gggatgcgtt catataaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
       61 tataacaagg cttgcagtgg aataatgaag ccgtttccgt gtccatcctt gttaaaacaa
      121 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
      181 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
      241 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
      301 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
      361 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
      421 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
      481 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
      541 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
      601 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
      661 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
      721 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
      781 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
      841 atgccgaaca acccgcgcgc gggtggcatt tctcgccgta tcgaaggcga cgaccgtacc
      901 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
      961 accgctggcg tcggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa
     1021 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ccccgttcct gattcatcag
     1081 gagagcaacg taatcgttcg cgctttccgc gattacttac gccaggacat cggcgaaatc
     1141 cttatcgata acccgaaagt gctcgaactg gcacgtcagc atatcgctgc attaggtcgc
     1201 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
     1261 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt
     1321 tcgattgtta tcgacagtac cgaagcgtta actgccatcg acatcaactc cgcacgcgcg
     1381 acccgcggcg gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag
     1441 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
     1501 atgacgcctg tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
     1561 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgt
     1621 cagcgcctga gcccatcact gggtgaatcc agtcatcacg tttgtccgcg ttgttctggt
     1681 actggcaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgtct gatcgaagaa
     1741 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtacc aatcgcttct
     1801 tacctgctga atgaaaaacg ttctgcggta aatgccattg aaactcgtca ggacggtgtg
     1861 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gctgcgcgtg
     1921 cgtaaagggg aagaaacccc aaccttaagc tacatgctgc cgaagctgca tgaagaagcg
     1981 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtccggaaca acctgcgctg
     2041 gcaacctttg ccatgccgga tgtgccgcca gcgccaacgc cagctgaacc tgccgcgcct
     2101 gttgtagctc cagcaccgaa agctgcaccg gcaacaccag cagctcctgc acaacctggg
     2161 ctgttgagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtagtga agaaaccaaa
     2221 ccgaccgagc aaccagcacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
     2281 aagcctcgtc agaacaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
     2341 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag
     2401 actgccgaga cgcgtgagag ccgtcagcaa gctgaggtaa cggaaaaagc acgtaccacc
     2461 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
     2521 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
     2581 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
     2641 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
     2701 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
     2761 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
     2821 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
     2881 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
     2941 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
     3001 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
     3061 atggtgactg aggtccctgt cgccgccgct gtcgaaccgg ttgttagcgc gccagttgtt
     3121 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
     3181 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
     3241 accgagtctg atgttgccgt agcccaggaa gttgcagaac acgcagaacc ggtggttgaa
     3301 ccgcaggaag agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgca
     3361 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt
     3421 gaaacggtag ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca
     3481 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcatcacag tgactggcag
     3541 cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat
     3601 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa
     3661 gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc
     3721 aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga
     3781 tccctgagcg ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt
     3841 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
     3901 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
     3961 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca
     4021 gttcgttcag ctgcgtgcct aattccgcgc gaacggcaag gacattattc agcgagtttt
     4081 tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat
     4141 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
     4201 cagaagcgct accgtctggc tccgcaactg cgttgctggt aatactgtcg aaaattttgt
     4261 cacccgtgtg ccctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
     4321 ctccaccgac gtatttccct ttctcttcgc taaacggcgc agtctctgtt ttataaccgg
     4381 caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
     4441 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
     4501 cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct
     4561 cttccagtga cactttttga atggcgaaag tacgcgccag cgtgtactgg ctgttttgcg
     4621 cctgtgcctg ggagagaact acggcttgtg atgctgcaat gggatcgtca gaagggttaa
     4681 cgactcgctt acctgtcgac atctgctcgc cgtacttcat ccattccgcc tgagaattgg
     4741 tgatgccacg catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc
     4801 ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt
     4861 cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcttcgtcga gattgacgcc
     4921 agagatagac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggcgctact
     4981 ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt
     5041 aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc
     5101 gttgcggtta tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc
     5161 ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc
     5221 gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt
     5281 ggcatccggc gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt
     5341 attatcgaac gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgtggc
     5401 accgatcgca acgtcacctt tgtttttcgt gttttgcaaa accgcgggct taccgatagc
     5461 aaagaaatct tcaccagcat cgccgttggc atcaaatccg gctttgtgtt gggtgttgaa
     5521 agcctcagca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg
     5581 agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc
     5641 aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga
     5701 aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccat
     5761 cgtgatgtta taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa
     5821 ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac
     5881 gcctgtcagg cgggagattt ggtcgttcag gctggcgatt tgtttagcgt aattgttgat
     5941 ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gaagatactg
     6001 atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc
     6061 tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg
     6121 tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata
     6181 gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt aactggttgg tgataaacgc
     6241 atcatactca cgctgcacac cagaaacgta gacaccattg ccaacccagc cgccagcgcc
     6301 caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata
     6361 gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat
     6421 ggcgttatta atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta
     6481 tcggcagcga cccgccggac ttgagttatt caaaacagat tatcaatgtt catactgtag
     6541 gttttgctca ccttgtcgct tatcgatttc atctgctgaa tcatgttggt gagtttgcgg
     6601 gcatagtgag gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca
     6661 ctcgtggcgg tggtcacggc ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc
     6721 gactatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg
     6781 ccgttttcat attcagtcgt ggtgatttca gtaactggcc ctttccagtt gccagaggct
     6841 ttgacaccaa acaggttata gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc
     6901 caaccagatt ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg
     6961 cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctttactgtc acccggcagc
     7021 gaatcatcgt agttacgtgg cacggccttt tgcaccagct gcgaaagcgc ctgattttga
     7081 taacgcacca cagtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc
     7141 aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc
     7201 gccgtcatct gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg
     7261 ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg
     7321 aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgcccgct
     7381 ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgcta
     7441 tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt
     7501 gcagtatgga catcagatcc atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt
     7561 tattgagcct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga
     7621 tctgcgtttg tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga
     7681 cattggcctg acgattaact gttactgaga gattcccctg cgctaccgcg cagctgtcga
     7741 gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt
     7801 cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaagcgg acctgggaac
     7861 tgttgccact cggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat
     7921 atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt
     7981 taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa
     8041 ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgccag
     8101 cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt
     8161 caacgccctt aagcggtgtc atcaacaacg tacctccacg caagcttttg gcattaccca
     8221 tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacgctg
     8281 tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc
     8341 ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt
     8401 caccggtgcc atccagcccc accaccagac catagccaat cagtgagttt tgccttaccc
     8461 cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgtg accagtagaa
     8521 gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag
     8581 gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta
     8641 ttcaatgcgc gcatccacca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg
     8701 tggattaacc acgccagaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt
     8761 ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt
     8821 gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga
     8881 ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc
     8941 aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct
     9001 gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc
     9061 aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc
     9121 gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga
     9181 gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg
     9241 cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac
     9301 cggttaagcc ttagagttgc gtcagttttt gctgcatctg atcggtggtg gacaccgctt
     9361 tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt
     9421 tgacgttaga cgtttcaaca tacccttgat acagcaatcc cgcgccgttc aggcccggcg
     9481 tgctttcgtt cggtgcacca gaggattgcg tttcggtgta gaggttttcg ccaatgctct
     9541 ccagcccggt gtcattcata aaggtggtga gattgagctg cccaacctga accggagctg
     9601 cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat
     9661 tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt
     9721 tctgatccac ctggaaagag ccgtcacggg tataggctga tgaaccatct ggcaacatca
     9781 cctggaaaaa gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacagat
     9841 ttccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc
     9901 cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa
     9961 gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt
    10021 tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata
    10081 atgaactgat cataaaatgt cctgtagtgg attagtgaca agcagagcag aagcacgcga
    10141 cagtgtgaat tccccctcac tctaaccctc tccccaaagg ggcgagggaa tagtccgcgc
    10201 gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg
    10261 gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct
    10321 ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga
    10381 catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc
    10441 gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag
    10501 tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat
    10561 tgtgccatcg gcagcaatag tgatttccgc tccttccggc acagcaattg gccccgcctc
    10621 gcctatcacc tgatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc
    10681 attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccagc catcctgctg
    10741 caacgcaacg tccagcgggc gcgaggtgta atctatcttg ccgggcgtca tgtctgcacc
    10801 cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    10861 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    10921 ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg
    10981 atccattgag ctatcccgtc agcgattagc gtaagttaac cagcgtgttg aggatctggt
    11041 cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga
    11101 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa
    11161 agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    11221 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    11281 ccagcagttg agtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt
    11341 gataactcac cagatcgccc ggtttgtagc cgttctgggg ggttgccaca atattgttag
    11401 cgccggtatt ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac
    11461 cgttaattgc gccggttgcg atattattcg ccatcgcacc atccactaat gtgccattag
    11521 cattaaattc cagtgttgtc gctgtcttcg caatgctgtt tggatcactg ctatcctggg
    11581 tgtagacctg ccagttatta tccccggtct tcacaaagta gacgctcatg tcatgagcat
    11641 taccctgact gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg
    11701 cgctgaatgg cgtaacagta ggaagcggat cactggaatt caggttgatc tgcatcgacg
    11761 ccgtggtggt agttttcgct gccatcaggg tattcgggat cgaaatattg gtcggattcg
    11821 ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt
    11881 gcatattcac caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca
    11941 ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg gctgatagca acgtccagac
    12001 ctcgcccggt gttggtggtc gtgccatctg taaagtcctg agtgataccg gcaactttta
    12061 cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa
    12121 agccgtaggt ggcggagttg gcaatattgt tgccaataac atcgaggtta gtggcggcag
    12181 cgtttaatcc gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt
    12241 aaattatctg ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat
    12301 taccgctgtt gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca
    12361 gttgtgtacc accgttactg gcggtaatcg ctacattgta agaaccgttc ggcgcagtgc
    12421 tgccatcagt caacgtaccg tcccaggtga aactgtgaac tccggcggtc agttcaccaa
    12481 tatcaatggt gcgcacaacc gcgccatttt tatcggtgat ggtggccgta actttgtctg
    12541 ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc
    12601 cggttcccgc aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc aggttactgg
    12661 cctgtaacga ctggctgtta tcaatctgtc cggaaataga tccgagcatg gtattgagtt
    12721 tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca
    12781 ttggattggt cgggtcctgg tttttcagct gcgccaccag caaagtcaga aaactgcttt
    12841 gtaaatctgc ggcgttgctg cccgtgagcg aactactgct ggtggtactg acgccggtat
    12901 ttgtcggatc ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt
    12961 aagggttttc agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct
    13021 gcgtgacgcc gacatggtgt taaccatctc tccgacaaca tcaacgttcg gcatttttac
    13081 gtagcccttt gcatccgcca gcggattacc cggttcataa accagtttgt ccggggcctg
    13141 actttctata acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    13201 ctggaatacc acctgttttg cccgatatgg ctgtccatcg ggaccggtca cgctatcagc
    13261 attcgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    13321 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    13381 catgcctttg atttgcccgc tcaacgcact aaggctcatc tggtattgca gttctgcggt
    13441 aggaggcgtc agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca gagcaaccac
    13501 actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc
    13561 gcgcgcctga taaccaaggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg
    13621 ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag
    13681 catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt
    13741 tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatgacc attggggacg
    13801 ggtacaattc acgttgtaga aatagctggg gggcgaaaat gctggcaata aaacgtagcg
    13861 tggcgatcat cgcgatactg ttcagtccgt taagtgcggc gagcaatctc acatcgcaat
    13921 tgcacacctt ttttagcgcc cagctcgtgg gggtaagtga tgaggttcgt gtttctattc
    13981 gtacagcacc caatctacta ccgccgtgcg agcagccatt gctttcgatg agcaataatt
    14041 cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac
    14101 aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcccatt gtgcggggag
    14161 gaaagctgga agctggcaat gtcaaactaa aacgcggacg actggatacc ctgccaccgc
    14221 gtacggtgct ggatatcaat caacttgttg atgccattag cctacgcgat ctatcacccg
    14281 atcaaccaat ccaattaacc cattttcgcc aggcatggcg ggtaaaagcg ggacaacgcg
    14341 tcaacgtgat cgccagcggt gacgggttta gcgccaacgc agaaggtcag gcgctgaaca
    14401 atgcagccgt cgcacagaat gcgcggatgc gcatggtatc gggactggtg gtcagcggcg
    14461 ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc
    14521 ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta
    14581 ttgatcgcac ttcgcctctg aatcccgtaa gcaccgttca accgcgcgaa accactgacg
    14641 cgccggtaac gaacacccgg gcggcaaaaa caaccgactc caccagcacc agcaccagca
    14701 ccagcaccag caccagcacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    14761 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    14821 gtaacggtga actaaaaatg gacatcggca aaattgccga tgcgctgatc aacgaagctc
    14881 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct tgaccagatg
    14941 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    15001 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    15061 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    15121 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    15181 cataacggtt ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    15241 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac aacccatcgc
    15301 ggcggtaaaa agatttcgat ctgaagatta tctccggcct gcactgcagg ccggaatgca
    15361 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    15421 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    15481 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    15541 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    15601 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    15661 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    15721 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    15781 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    15841 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    15901 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca
    15961 aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    16021 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggacggaaa
    16081 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    16141 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    16201 catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag
    16261 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt
    16321 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    16381 accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca
    16441 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    16501 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtatcagcga agcctggcgc
    16561 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    16621 aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    16681 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    16741 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    16801 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    16861 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccagaccaa
    16921 cgggcaaaac gcctgcaaga caggcgctga aagaggcgaa tagtctacct ggattatggt
    16981 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    17041 caacgatacg ttgcgccagc acggcctatt cgccggcggt ttgcggaact gtctgatttt
    17101 gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgttca agagtgctct
    17161 gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca
    17221 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    17281 cggcacggcg atgcatacag gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg
    17341 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    17401 cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    17461 aaagatcgtg tggcccgaca ctattgctac gatgtttatc cattcttagc gaagctgcgg
    17521 tagcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca
    17581 tcaacgtcag ttttttccgc gccgccagtt ccaccagccg ttcagcatcg cgcagatttt
    17641 ctgccagcgg tttatcgaca cagacatgta cacccgcatt gagtaacgta ctgaccacgt
    17701 caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg
    17761 ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg
    17821 taggcgacca ggctccttgt aacatccagt cagacgctgc cgccagcacc ggtaaccacg
    17881 ctttttgcgc aatgccgcct aatcccacaa cgccgatacg taattttttc actttaatct
    17941 cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc gatttccagg
    18001 gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt taccgccggc
    18061 tgatcctcta cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    18121 cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc cagcgtcgac
    18181 tccaattccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc
    18241 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc tgcgctcagt
    18301 ttcagatcgc caaattctga attacaaaaa cgttgttcat atttggtgac ccgattacca
    18361 aagccactca ccgtgcgcag ataatggcgt ttgaccagat tatccagttg ctcctgcact
    18421 tcagattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    18481 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    18541 ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    18601 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    18661 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    18721 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    18781 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    18841 cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat tggaaaaatt
    18901 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    18961 ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg gataacagtg
    19021 gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    19081 cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    19141 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    19201 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    19261 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    19321 gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    19381 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    19441 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    19501 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    19561 tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc
    19621 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    19681 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    19741 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    19801 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg gtgcggtcat
    19861 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    19921 ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    19981 ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    20041 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgcc
    20101 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    20161 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    20221 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    20281 catggtcagc ggcctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    20341 cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg
    20401 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    20461 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    20521 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    20581 cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    20641 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    20701 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    20761 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    20821 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    20881 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    20941 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    21001 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    21061 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    21121 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggagaact
    21181 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    21241 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    21301 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    21361 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    21421 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    21481 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    21541 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    21601 tacccggcgt ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg
    21661 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    21721 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    21781 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    21841 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    21901 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    21961 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    22021 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    22081 ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc
    22141 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    22201 taaaaactgt catgccgtat accagcgaaa tttatggacg ggctattgta atgcccaatc
    22261 tggctccgcc cgtgaccact gttgaggctg ccgtggcgta tcgccagcgt attcttgatg
    22321 ccgtacctgc cgggcacaat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    22381 atcctaatga gctggagcgc ggatttaacg aaggcgtgtt caccgctgca aaactttatc
    22441 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    22501 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    22561 cagatatcga catttttgat cgtgaagcgc actttataga aagcgtgatg gaacctctgc
    22621 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    22681 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    22741 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    22801 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    22861 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    22921 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    22981 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct
    23041 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    23101 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    23161 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac
    23221 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    23281 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    23341 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    23401 attggcgtaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    23461 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    23521 ccccggcttt tttattttac ttacaaattt tgagtacaaa atttctacac ttcctcaaaa
    23581 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    23641 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    23701 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    23761 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    23821 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    23881 gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac
    23941 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    24001 tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    24061 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    24121 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    24181 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    24241 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    24301 ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg
    24361 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    24421 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    24481 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    24541 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    24601 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    24661 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaaccgttg acggtgagta
    24721 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    24781 gcctgtccag cctgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    24841 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    24901 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    24961 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    25021 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    25081 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    25141 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    25201 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    25261 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    25321 cggctgttgc actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc
    25381 tggggattgt tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg
    25441 acataaactg acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgagatta
    25501 ttcctgcttc tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag
    25561 attctttcat tattaattca aaattattga aataaaaata aatatcattc aatttgttat
    25621 ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg
    25681 atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc
    25741 attggctaag cgcaattatt gtctacggca tgtttacttt aggtctgtgg atggtcacgc
    25801 tcagttatta cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt
    25861 tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc
    25921 cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa ggctggacat ctcgcattgt
    25981 acctattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac
    26041 cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg
    26101 cagactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtccgtta
    26161 tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca
    26221 tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt
    26281 taaccttcgc gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg
    26341 acaaagaagg tcagcacgcc tttgttaatt tccgcatcca gcaccttggc tatagctggt
    26401 tatacggcac ctttgaagat ttcgacggta cttttacctt tgacgaaaaa aatccggctg
    26461 ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaatcac gccgaacgcg
    26521 ataaacatct tcgcagtgca gatttcctca ataccgcaaa atatccacag gcaacattca
    26581 cctccaccag cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga
    26641 atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc gacgacccat
    26701 ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag gacttcaata
    26761 tcaagacaga tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg
    26821 tacagcagaa gtaagcgtga aatgttgccg gatggcacgc atccggcaat gatatttatt
    26881 ctgttggatc aggaatgccc agtgttgtat tcagacgtcc acgagactta ttaaagatct
    26941 tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt
    27001 cttcgcagca aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa
    27061 taaacagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc
    27121 actggtggca atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa
    27181 caaaattttt gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgg
//
LOCUS       DAJVTY010000039        27160 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVTY010000039 DAJVTY010000000
VERSION     DAJVTY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27160
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.039"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..521)
                     /gene="ppa"
                     /locus_tag="OQQ66_001505"
     CDS             complement(<1..521)
                     /gene="ppa"
                     /locus_tag="OQQ66_001505"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1904479.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERA"
     gene            831..1787
                     /gene="ytfQ"
                     /locus_tag="OQQ66_001506"
     CDS             831..1787
                     /gene="ytfQ"
                     /locus_tag="OQQ66_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS1904480.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSPLYLPDTAKEELEKKKNMGY"
     gene            1927..3429
                     /locus_tag="OQQ66_001507"
                     /pseudo
     CDS             1927..3429
                     /locus_tag="OQQ66_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3443..4465
                     /locus_tag="OQQ66_001508"
     CDS             3443..4465
                     /locus_tag="OQQ66_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1904481.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4452..5447
                     /gene="yjfF"
                     /locus_tag="OQQ66_001509"
     CDS             4452..5447
                     /gene="yjfF"
                     /locus_tag="OQQ66_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1904482.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5480..6478)
                     /gene="fbp"
                     /locus_tag="OQQ66_001510"
     CDS             complement(5480..6478)
                     /gene="fbp"
                     /locus_tag="OQQ66_001510"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1904483.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6654..8027
                     /gene="mpl"
                     /locus_tag="OQQ66_001511"
     CDS             6654..8027
                     /gene="mpl"
                     /locus_tag="OQQ66_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1904484.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8110..8661)
                     /locus_tag="OQQ66_001512"
     CDS             complement(8110..8661)
                     /locus_tag="OQQ66_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1904485.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8755..10107
                     /gene="pmbA"
                     /locus_tag="OQQ66_001513"
     CDS             8755..10107
                     /gene="pmbA"
                     /locus_tag="OQQ66_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1904486.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10291..10677
                     /gene="cybC"
                     /locus_tag="OQQ66_001514"
     CDS             10291..10677
                     /gene="cybC"
                     /locus_tag="OQQ66_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1904487.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10722..11186)
                     /gene="nrdG"
                     /locus_tag="OQQ66_001515"
     CDS             complement(10722..11186)
                     /gene="nrdG"
                     /locus_tag="OQQ66_001515"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1904488.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11376..13514)
                     /gene="nrdD"
                     /locus_tag="OQQ66_001516"
     CDS             complement(11376..13514)
                     /gene="nrdD"
                     /locus_tag="OQQ66_001516"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1904489.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(13908..15563)
                     /gene="treC"
                     /locus_tag="OQQ66_001517"
     CDS             complement(13908..15563)
                     /gene="treC"
                     /locus_tag="OQQ66_001517"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1904490.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15613..17034)
                     /gene="treB"
                     /locus_tag="OQQ66_001518"
     CDS             complement(15613..17034)
                     /gene="treB"
                     /locus_tag="OQQ66_001518"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1904491.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17135..18100)
                     /gene="treR"
                     /locus_tag="OQQ66_001519"
     CDS             complement(17135..18100)
                     /gene="treR"
                     /locus_tag="OQQ66_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1904492.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18285..18338
                     /gene="mgtL"
                     /locus_tag="OQQ66_001520"
     CDS             18285..18338
                     /gene="mgtL"
                     /locus_tag="OQQ66_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1904493.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18479..21175
                     /gene="mgtA"
                     /locus_tag="OQQ66_001521"
     CDS             18479..21175
                     /gene="mgtA"
                     /locus_tag="OQQ66_001521"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1904494.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21381..21767)
                     /gene="ridA"
                     /locus_tag="OQQ66_001522"
     CDS             complement(21381..21767)
                     /gene="ridA"
                     /locus_tag="OQQ66_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1904495.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21840..22301)
                     /gene="pyrI"
                     /locus_tag="OQQ66_001523"
     CDS             complement(21840..22301)
                     /gene="pyrI"
                     /locus_tag="OQQ66_001523"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1904496.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22314..23249)
                     /gene="pyrB"
                     /locus_tag="OQQ66_001524"
     CDS             complement(22314..23249)
                     /gene="pyrB"
                     /locus_tag="OQQ66_001524"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1904497.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQSELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23253..23387)
                     /gene="pyrL"
                     /locus_tag="OQQ66_001525"
     CDS             complement(23253..23387)
                     /gene="pyrL"
                     /locus_tag="OQQ66_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1904498.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23667..24062)
                     /locus_tag="OQQ66_001526"
     CDS             complement(23667..24062)
                     /locus_tag="OQQ66_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1904499.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24193..24906)
                     /gene="bdcA"
                     /locus_tag="OQQ66_001527"
     CDS             complement(24193..24906)
                     /gene="bdcA"
                     /locus_tag="OQQ66_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1904500.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            24977..25570
                     /locus_tag="OQQ66_001528"
     CDS             24977..25570
                     /locus_tag="OQQ66_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1904501.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25715..26167
                     /gene="tabA"
                     /locus_tag="OQQ66_001529"
     CDS             25715..26167
                     /gene="tabA"
                     /locus_tag="OQQ66_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1904502.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26290..>27160
                     /locus_tag="OQQ66_001530"
     CDS             26290..>27160
                     /locus_tag="OQQ66_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1904503.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIIKF
                     VMNNPEGERADLSKVEIEITLSTFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILI"
BASE COUNT         6473 a         6764 c         7216 g         6707 t
ORIGIN      
        1 tttgcgcgct cgaaggaagc aacgatttca gctttagcgg cttctgcgtt ttcccaacct
       61 tcaactttca cccacttgcc tttttcgagg tctttgtagt gctcgaagaa gtgagcgatt
      121 tgcgctttca gcagttcagg cagatcgttt acgtctttaa tgtgatcgta ttctttgctc
      181 agcttgctgt gcggaaccgc aaccagtttc gcatcttcac cggcttcgtc ggtcattttc
      241 agaacgccaa ccggacggca acggatcaca gaaccaggct gcagcgggta cggagtcggg
      301 accagtacgt caaccgggtc accgtccaga gacagggtgt ggttgatgta accgtagttg
      361 cacggataga acatcgcggt ggacatgaag cggtcaacga acagtgcgcc gctctctttg
      421 tcgatttcgt atttgatcgg atctgcgtta gccgggatct caataacaac gtagatgtct
      481 tccggcagat ctttacccgc agggacgttg agtaagctca tgtctgtttc ctttaaaaat
      541 atgtggcaaa caagtgccga gtattatagc caactcgcgc cgaatgtctt tgcttgtttt
      601 cgtcttcatt ttcctttttc gccagtttta agacggtata cagagcagga aaatccataa
      661 ccgtagccgc atttttcata gtgagatgaa agcgattaca aacttgtgat taacgtttta
      721 tttacttttt tgaagtgtga tgtaacgcaa tccgttacat aacgaattgt ctatagtttt
      781 ttcgcgaaca tcttttcacc aataataatt accccgacga ggacaaccct atgtggaaac
      841 gcttacttgt agtctctgca gtctcggcag ccatgtcgtc tatggcgttg gccgctccat
      901 taaccgtagg attttcgcag gtcggatcgg aatccggctg gcgcgccgca gaaaccaatg
      961 tggcgaaaag tgaggccgaa aaacgcggaa ttacgttgaa aattgccgat ggtcagcaaa
     1021 agcaggaaaa ccggattaaa gcggtacgtt ccttcgttgc acaaggggtg gatgcgatct
     1081 ttatcgctcc agtggttgcg acgggttggg aaccggtatt aaaagaggcg aaagatgccg
     1141 aaatcccggt cttcttgctc gaccgttcca tcgatgtgaa agacaaatct ctctatatga
     1201 ccaccgttac tgccgacaac atcctcgaag gtaagttgat cggtgactgg ctggtaaaag
     1261 aagtgaatgg caaaccatgc aacgtggtgg agctgcaggg caccgttggg gccagcgtcg
     1321 ccattgaccg taagaaaggc tttgccgaag ccattaagaa tgcgccaaat atcaaaatta
     1381 tccgctcgca gtcaggtgac ttcacccgca gtaaaggcaa agaagtcatg gagagcttta
     1441 tcaaagcgga aaacaacggc aaaaacatct gcatggttta cgcccataac gacgacatgg
     1501 tgattggtgc aattcaggca attaaagaag cgggcctgaa accgggcaaa gatattctga
     1561 caggttctat cgatggcgta ccggatatct acaaagcgat gattgatggc gaagcgaacg
     1621 ccagtgttga actgacgccg aatatggcgg gacccgcttt tgacgcgctg gagaaataca
     1681 aaaaagacgg caccatgcct gaaaagctga cgttaaccaa atcccccctt tacctgcctg
     1741 ataccgcaaa agaagagtta gagaagaaga aaaatatggg gtattgaggg ttgctatgcc
     1801 tgatgccgat tcgtaggccg gataaggcgc tcgcgccgca tccggcgatg gtgcactgaa
     1861 gcctgatgcg acgcttaccg cgtcttatca tgcctactgg gagcacgctt tacaccgggg
     1921 gaaaccatga cgaccgacca acaccaggaa atcctccgca ccgaaggatt aagtaaattt
     1981 ttccccggcg tcaaagcgtt agacaacgtt gatttcagcc tgcgccgtgg cgaaatcatg
     2041 gcgctgctcg gtgaaaacgg ggcgggaaaa tcaacgctaa tcaaagcatt aactggtgta
     2101 taccacgccg atcgcggcac catctggctg gaaggccagg ttatctcacc gaaaaatacc
     2161 gcccacgcgc aacaactcgg cattggcacc gtctatcagg aagtcaacct gctacccaat
     2221 atgtcggtcg ctgataatct atttataggc cgcgaaccca aacgcttcgg ccttctacgc
     2281 cgcaaagaga tggaaaagcg cgccaccgaa ctgatggcat cttacggttt ctccctcgac
     2341 gtgcgcgaac cgctcaaccg cttttcagtc gcgatgcagc aaatcgtcgc catttgccgg
     2401 gctatcgatc tctccgccaa agtgctgatc ctcgatgaac ccaccgccag cctcgacacc
     2461 taggaagtgg agctattatt tggcctgatg cgtcagttgc gcgatcgcgg cgtcagcctg
     2521 atctttgtca ctcactttct cgatcagagc tatcaggtca gcgatcggat caccgtttta
     2581 cgcaacggca gtttcgtagg ctgtcgggaa acgcgcgagc taccgcagat cgaactggta
     2641 aaaatgatgc tggggcgcga gctggatacc cacgcgctac agcgtgccgg gcgaacattg
     2701 ttgagcgaca aacccgttgc cgcgttcaaa aattacggca aaaaaggaac gatagcaccg
     2761 tttgatctcg aagtgcgccc cggcgagatc gtcggcctgg caggcttgct gggatccgga
     2821 cgtaccgaaa ccgccgaagt gatcttcggt atcaaacctg ctgacagcgg cacggcgttg
     2881 atcaaaggca aaccgcaaaa cctgcgatcg ccacatcagg cttcggtact gggcatcggc
     2941 ttctgcccgg aagacaggaa aaccgatggc atcatcgctg ccgcctcggt gcgggaaaat
     3001 atcatcctcg ctctccaggc ccagcgcggc tggctacgac ctatttcccg caaagagcag
     3061 caagagattg ccgaacgctt tatccgccag cttggcattc gcacaccttc aactgaacaa
     3121 ccgattgaat ttctctccgg cggcaatcag caaaaagtgt tgctttcacg ttggctactg
     3181 acccgaccgc aatttctgat cctcgatgag ccaacccgtg gcattgatgt tggtgcccac
     3241 gccgagatca tccgcctgat tgaaacgcta tgcgccgatg gtctggcgct gctggtgatc
     3301 tcctccgaac tggaagaact ggtgggctat gccgaccggg tgatcatcat gcgcgatcgc
     3361 aaacaggtgg cggagatccg gctggcagag ctttccgttc cggctatcat gaacgccatt
     3421 gcggcgtaag gagaacagtg tgatgcctca atctcttccg gacaccacta cgccaaaaag
     3481 gcgctttcgc tggccgacgg ggatgccgca gctggtagca ctattgctgg tgctgctggt
     3541 cgacagcctg gtagccccgc atttctggca ggtagtgctt caggatgggc gtttgttcgg
     3601 tagccccata gacattctta accgtgcagc ccccgttgcg ttactggcga ttggtatgac
     3661 gctggtgatc gccaccggtg ggattgatct ctccgtaggg gcggtgatgg ctatcgccgg
     3721 agccacaacg gctgcgatga cggtcgcgga agtcagcctg ccgattgttt tgttaagcgc
     3781 cctaggcacc ggcatcctgg cgggattgtg gaacggcata ctggtagcga tcctcaaaat
     3841 tcagccattt gtcgccaccc tgatcctgat ggtcgccggg cgcggcgtgg cgcaactgat
     3901 cacctccgga cagatcgtca cgtttaactc gccggatctc tcatggtttg gcagtggatc
     3961 gctgttgttc ctgccaacgc cagtcattat tgcggtactg acgcttctcc tgttctggct
     4021 gttgacccgc aaaacggcac tggggatgtt tatcgaagcc gttggtatca acattcgggc
     4081 ggcaaaaaat gccggggtaa acacgcggat catcgtcatg ctcacttatg tgttgagcgg
     4141 gctgtgtgcg gcgattgcgg gcattatcgt ggcggcggat attcgcggtg ccgatgccaa
     4201 caacgccgga ttatggctgg agctggacgc cattctcgcg gtggtgattg gcggcggatc
     4261 gctgatgggc gggcgcttta acctgctgct ctcggtagtg ggggcgctga ttattcaggg
     4321 gatgaacacc ggaattttgc tttcgggctt tccaccggag atgaaccagg tggtgaaagc
     4381 ggtggtggtg ctttgcgtgc tgattgtcca gtcgcaacgc tttatcagtc tgattaaagg
     4441 agtacgtagc cgtgataaaa cgtaatttgc cactgatgat caccatcggc gtctttgtgc
     4501 tgggctattt ctactgcctg acgcagtttc ccggttttgc ttccacaaga gtgatctgca
     4561 atatcctgac cgataacgct tttctgggga ttattgccgt tggtatgacc tttgtgatcc
     4621 tctctggcgg tatcgatctc tccgtcggtt cggtgatcgc ctttactggc gtgtttctgg
     4681 caaaagtggt tggcgatttc ggcctctcgc cgctgctggc gtttccgcta gtgctggtga
     4741 tgggctgtgc cttcggcgca tttatggggc ttctgatcga cgccctgaag atcccggcat
     4801 ttatcattac gcttgcgggg atgttctttt tgcgcggcgt cagctatctc gtttcggaag
     4861 agtcgattcc gataaaccat cccatttatg acacgctctc aagccttgcg tggaaaatcc
     4921 ccggcggcgg tcgccttagt gcgatggggc tgttgatgct ggcggtggtg gttatcggta
     4981 ttttccttgc gcatcgtacc cgttttggca atcaggtgta cgctattggc ggcaacgcga
     5041 cgtcggcgaa cctgatgggg atttctactc gcagcactac tattcgcatt tatatgctct
     5101 ccaccggact ggcaacactg gcggggattg tcttctcgat ttatacccag gccggatatg
     5161 cactggcggg cgtaggtgtg gaactggacg ccatcgcgtc ggtggtgatt ggcggtacgt
     5221 tgttaagtgg tggcgtcggg acggtgcttg ggacgctttt tggcgtggca attcagggac
     5281 tgatccagac ttacatcaac tttgacggca cgctgagttc ctggtggacg aaaatcgcca
     5341 tcggtatttt gttgtttatt tttatagcat tacagcgtgg gttaacggtg ctgtgggaga
     5401 atcgtcagag ttcgccagtg acaagagtca acattgcgca gcgataaaaa tgcctctccg
     5461 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
     5521 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
     5581 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
     5641 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
     5701 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
     5761 tataacgtga ggtatacggg cggttggtgg atttatcttc ttcctggcag aatttaatgt
     5821 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
     5881 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
     5941 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
     6001 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
     6061 gcgtgccgac gggcgtaact cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
     6121 catcgatgtt ggacgagcca tccagtgggt ccatcagcac cacgtatttt gcatgttcac
     6181 agccttcaaa gacaacaatc tcatcttctt cttcagaggc aatgcctgca acgatatcgc
     6241 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
     6301 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
     6361 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
     6421 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     6481 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     6541 atgttgcgct caggtgaatc gcgccagcaa attacggatt atcctgaaat gcgtttctca
     6601 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     6661 ttcatatttt aggaatttgt ggcacgttta tgggcggtct ggcgatgctg gcgcgccagt
     6721 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     6781 agaagcaagg cattgaactg attcagggtt acgatgccag ccagctcgat ccgcagccgg
     6841 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     6901 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     6961 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     7021 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     7081 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     7141 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     7201 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     7261 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     7321 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     7381 agcaggggca ctggcaggcg aaaaagctga ccactgatgc ttccgaatgg gaagtcttgc
     7441 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcata
     7501 atggcctgat ggcgattgcg gcggcccgcc atgttggcgt agcgccggca gataccgcta
     7561 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     7621 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     7681 cgctgcgtgg caaagttggt ggtacagcgc gcattattgc tgtgctggaa ccgcgctcga
     7741 ataccatgaa aatggggatc tgcaaagacg atttggcacc ttcattaggt cgtgccgatg
     7801 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     7861 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     7921 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     7981 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcctcagcc
     8041 tgagatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     8101 caacgaaaat tactcttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     8161 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gagtacgcag
     8221 ttgctgacga tcggcatcgg gccacagatt taatacctca gcgatggcgt catcaccttg
     8281 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     8341 acggtttttc agcttatcca gcgcctgacg gatcggttct acatcgcgct ggcgcagcat
     8401 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     8461 aatagccgcg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     8521 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     8581 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     8641 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     8701 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     8761 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     8821 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     8881 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagc
     8941 gatggcgcgc tggggatcac tgtttatcac cagaaccgca aaggtagcgc atcatccacc
     9001 gatttaagcc cgcaggccat tgcccgtact gtacaggcgg cgctggatat tgcccgttat
     9061 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcacca
     9121 gatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     9181 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     9241 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     9301 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     9361 cgtgattacg cctacaccat tggtcgtgcg atgagcgatc tgcaaacgcc agagtgggtt
     9421 ggggtcgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     9481 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     9541 gcgatagcgg gtggatcggt ttaccgtaaa tctaccttcc tgctggattc gctgggtaaa
     9601 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     9661 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     9721 atcctgactc agtggctgct gaccagctat tcggcgcgaa aactggggct gaaaagcacc
     9781 ggacatgcgg gcggtattca caactggcgg attgccggac aaggtctaag cttcgagcag
     9841 atgctcaaag agatgggcac cgggctggtg gtgacggaat tgatgggcca gggcgtgagt
     9901 gcaattaccg gtgattattc ccgtggtgcg gcgggcttct gggtagaaaa cggcgaaatt
     9961 cagtatccgg ttagcgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
    10021 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
    10081 ccggagatga aaatcgccgg acagtaaaaa aactggcgcg acctgtcgcg ctataaacta
    10141 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    10201 cgtactcgca gtacgtgacc cggtcgaatg agcgtagtcg tcgatgaggc agtgtgaatg
    10261 atgaagtgta aataaaaaag gaagtgagca atgcgaaaaa gcctgttagc tattcttgca
    10321 gtctcctcgt tggtattcag ttcggcgtcg tttgccgctg atcttgaaga caatatggaa
    10381 accctcaacg acactttaaa agtggtcgaa aaagccgata acgcggcgca agtcaaagac
    10441 gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc
    10501 gaaggcaaat caccggacag cccggagatg aaagacttcc gtcacggttt cgatattctg
    10561 gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag
    10621 gctgccgcag agcaactgaa aacgacccgc aacgcctatc accagaagta tcgttaattc
    10681 ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa atgatgcacc
    10741 acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg cacaaatttg
    10801 ccgtcgacca gcacgttaat caaatcaaca acctgcattt gcgctgcgtt gagttcgtcg
    10861 agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg gatgcgttgt
    10921 accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc ggagagggaa
    10981 ataccctggc gtttgatacg agtgtcattc agatcgttaa tgatctggtc ttccattgct
    11041 ttagtaaatg gctgaccgga atttacccgc catgtgcttt tgttatagca accggggcat
    11101 tcatgaacac acccggagac aaacagagtg caacgagtgc cggggccgtt gacgatgtcg
    11161 acaggatagt actgatgata attcattatt tcgcctggct tatcagtccg cgtgtagact
    11221 gcatccggta atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg
    11281 ccaaatgtag gccggataag gcgtttacgc cgcatccggc aatcaacgcg ggatgcgacg
    11341 ctttcacatc acatccggca tttgcttagt gcagatcaac ctatctgccc atttcccaaa
    11401 tgtttaacgc ggcgcttaac ttcttcctgc ttgccggcgt taaacggacg tgcatccggg
    11461 ctacctaaat atccgcacac gcggcgagtt accgacacac gggaggcgtc atggttacca
    11521 catttcgggc aggtgaagcc tttgctggtg cactcgaact caccggtaaa gccacactcg
    11581 tagcactcat caatcggtgt attggtgccg taatacggta catgctgata gctgtaatcc
    11641 cagacatctt ccagcgcctt caggttgtgc tgaatgtttg aatactcgcc gtagcaaatg
    11701 aaaccaccgt tcgccagcgg cgggtaaggc gcttcaaagt cgatcttgtc gtacgggttc
    11761 accttcttct ccacatcgag gtggaaactg ttggtgtagt aacctttatc ggtcacgccc
    11821 ggcaccacgc caaactcagc agtatcgaga cggcagaagc ggtcgcacag gttttcactc
    11881 ggcgtgctgt agagactgaa accataaccc gtttcttcct tccactgatc cactgcctga
    11941 cgcagacgtt caacaatggc gatacctttc gcgcgaagct gctcgttgtc gtaaacatgc
    12001 tcgccgccga acagcgcgtt aatggtttcg tggatgccga tgtaacccag cgaaatagac
    12061 gcacgaccgt ttttgaagat ttcagaaaca tcatcgtcag cattgagacg cacgccacaa
    12121 gcaccttcca tatagaggat cggggccacg cgcgctttca cgccttcgag acgagcgata
    12181 cgggtcatca gcgccttacg tgccagcacc agacgttcat ccagcagctt ccagaaggtg
    12241 gcttcatcgc cttttgcttc cagagcaata cgcggcaggt tcaggctgat cacgccgagg
    12301 ttgttacgac catcgtggat ctgctcgccg ttttcatttt cccacacgcc gaggaagctg
    12361 cggcagccca tcggagtttt aaacgaaccg gtgactttca ctacctgatc gtagttcagg
    12421 atatccggat acatgcgctt gcttgcgcac tccagcgcca gctgtttgat gtcgtagttc
    12481 ggatcgcctt ttttatggtt caggccatcg cgaatcgcaa acaccagttt cgggaacacc
    12541 gcagttttac ggtttttacc gaggcctgcg atacggttac gcaggataga ttcctgaatc
    12601 aggcgcgact cccagctggt gcccagacca aaaccaaagg ttacaaacgg cgtctgaccg
    12661 ttggcggtgt gcagggtgtt tacttcgtac tccagcgact ggaaggcatc gtagcactct
    12721 ttaatggtac gagagttagc gtagccttcg gcgtccggga tgttccactc ttctgcggtc
    12781 ttgcgatgtt tgttgtagct ggcggtgaca aacggtgcca gcacttcatc gatacggtta
    12841 atggtggtgc cgccataaat atggctggcg acctgagcaa taatctgcgc agttacagcg
    12901 gttgccgtag agatcgactt cggcggttca atctcggcgt tccccatctt aaagccctgg
    12961 gtcagcatgc ctttcaggtc gatcaacatg cagttgaaca tcgggaagaa cggcgagtaa
    13021 tcgagatcgt gatagtgaat atcgccacgc tcatgtgcct gcaccacgtc acgcggcagc
    13081 aggtgctgac gtgcatagtg tttagccacg atcccggcca gcaggtcgcg ctgggttgga
    13141 atcaccttgc tgtctttgtt ggcgttttcg ttgagtaacg aggcgttggt ctgctcgacc
    13201 agaccacgga tctcctgatt caggcgaccg cgtttttcac gttcaatgtc gcgatcgtga
    13261 cggtactcga tgtaagcacg agccagttgt ttgtatggac ctgacatcag ctggttttca
    13321 acagcggtct ggatctcatt gatatccacc tggttgcggc cctgcatctg ctcgctgaca
    13381 actgcagcaa cagtggcgca ataatcggca tcatcgactt ccgctgcttt agctgcacgc
    13441 agaatcgctt ctttgatgcg ctctgattta aacggcactt tgcagccgtc tcgtttcatc
    13501 acatgcggtg tcatgatcgc tccatatttt taagaacagg ttatccacag aaattgggaa
    13561 aggcgttttc cggttttttc gtttcgtttt ccggtgcttt ccgcaatcgc catccgcttt
    13621 tccacaacgc cgcacctttt tcgtggggag ctgtagtagc aattatagtc gattaataca
    13681 acatattggg ttgggacgca ttttaaagtc tatatatagt gctttgcatc aaggatgttt
    13741 gaactttttt tgatgtagct caaagtaaaa agcagagcgt acggatgacg ggcgctacag
    13801 cgttatgtaa attttttaat gaatttgctg gttgaaaaat aaacaaaaac aacatgctga
    13861 cagacaaaac cccggaatga ccggcattac cggggcttag ggaagattta cttctgtaac
    13921 caccagacag cctcaaaagg ccgtaaattc atggcacagg gttgtggtga ggcttcttcg
    13981 tagttatgca tcacaagctg ccagttgccg cgcatttgcc ctggctgcca gggttggatc
    14041 tcacggctaa ggttggcaat gaccagcaag gtttgcccct tccattcacg gcgatagcac
    14101 cacaatacag ggctgtttgg cagcagatcc tggtaattgc cccatgtcag gatggcttcc
    14161 tgcttacgca gtgcgattaa cttttggtag gtgtaaaaca ccgaggaatc atcggccagc
    14221 gcggcttcta cgttgatttg ttgatagtta tcacccaggc caatccacgg ttcgccagcc
    14281 gtaaatccgg cattatcgcc gttgctccat tgcatgggcg tgcggctgtt gtcacgggat
    14341 ttactggcga ggattgccaa taactcgtcg gcatcacgac catcgttgcg cagctcggca
    14401 aacatattga ggctctctac gtcgcgatag tcagtaatgc gcgtgaaatg cgggttggtc
    14461 atgccaatct cttcgccctg gtagatatac ggcgttccct gcatgccatg cagcaccatc
    14521 gccagcattt ttgccgcagg cacgcggtat tcaccttcat caccaaagcg agaaacaatg
    14581 cgcggctgat cgtggttaca ccagaacaag gcattccatg ctacgttgtg cattccttgt
    14641 tgccagtggc ggaacaatgt tttcaacgcc acaaagtcag gtttagccag cgtccatttt
    14701 tcaccaccgg gataatcgac cttcaggtga tgaaaattaa aggtcatcga caattcactg
    14761 cctgtcagtg ccgcgtatcg ctggcaatgc tcaaggctgg tggaggacat ttcacctacg
    14821 gtcattaacc cgcgtggcgt aaacacatcg cggttcatct cgtgcaaaaa ctcgtgtgct
    14881 cgtggcccgt cggtgtagaa gcgacgcccg tcgccatcca ggtcatcagg gaaacgcggg
    14941 tctttggaga tcagattcac cacatccagg cgcaacccgt cgaccccacg atcggcccag
    15001 aactcacaga cttttttcag cactgcgcgt accgctggat tctcccagtt gagatccgcc
    15061 tgttctggtg caaagagatg caaatagtac tgttcgcttt ccgcatgcca gcgccacgca
    15121 ctaccgccaa attttgaacg ccagttgttc ggtggcgttt ctggttctcc atcgcgccag
    15181 atataaaact ggcggtaagg gctttctttg ttcagcgcct cgcgaaacca ggcatgttgg
    15241 gtagaggtat ggttaaacac catatcgaga atgatacgaa tcccgcgcga ttttgcctgc
    15301 gtcaccagtt cgtcaaaatc gtccagcgtg ccgtaggtgg gatcaatcgc cgtatagttc
    15361 gctacgtcgt aaccgttatc gacctgggga gagacataaa agggggttag ccagatggca
    15421 tcaacgccca gtttatgcag atagtccagg cgttggataa cgccacgtaa atcgccggta
    15481 ccgctacccg tggtgtcctg aaaactcttt ggataaatct ggtagataac gccgttttgc
    15541 caccagtggg gaggattagt cattacgtta ttcctgcaaa tgcgaggggg cgcaattgcg
    15601 ccccgaagaa aattaaacaa tgtccagcgt gcccaggcgg tatttccgct gatagataaa
    15661 cgaggtgagt acaatcggga tgatgatggc gatagccatt gccagcgcaa acacctgcca
    15721 gtagttcggt tgaatcgaga gaattcccgg caggccgcct acgccgatgc cattcgccat
    15781 aacgccgttc aggccgcata gcaatcctgc cagaccagaa ccaatcatcg cgcacagcat
    15841 cgggaagcga tatttcaggt tgatgccgta cattgcaggc tcagtgaccc caagccaggc
    15901 ggagatcgcc gcaggcacgg agatctcgcg ttcattgtgc ttgcggctgg aaatgataat
    15961 gccgatcacg gcggagccct gagcgatatt cgacagcgca atcagtggcc acactggcgt
    16021 accacccatg ctttgaatca tctgcaaatc aatagcaagc gtggtctggt gtaccccggt
    16081 gatcaccagc ggggcgtaca ggaagccaaa caatgccgcg ccaatcggag caaagctgcc
    16141 ggtcatcagg tgacgtaccg caaaggcaac gccatcgcca atcatgcgac caaacggacc
    16201 aatcagcgca tgggcgagga acaccgcgag gatcagcgaa cagacgggca ccaccaccag
    16261 atagaggtaa tctggcacga tgcgtttaag gcgagtttca ataacgccca gcgccagccc
    16321 ggctaacagt gccgggatca cctgcgcctg atagcccact ttggcgatgc tgaacatgcc
    16381 aaagtcccac acttccggca gctgctgccc gagcagataa gcgttcatca gttgggggga
    16441 aaccagtgtc acgccaagca cgataccaag gatcggtgtg ccgcccattt ttttcaccgc
    16501 tgaccagcaa atcccgaccg gcaggtagaa gaagatcgct tcaccgatca accacagaaa
    16561 atcgtagatc gtttgcaggg aagggtacat ttgcgccagc gtttgaccgt tgctcatggg
    16621 caaatcgccg atcacattgc gaaaaccgag gatcaaaccg ccgctaatca acgcgggcag
    16681 caacgggaag aaggtctctg cgaaatgaga gatcaactgc tcatgccatt tcatattctg
    16741 ccgggcggct ttttttacct gctctttatc aacctgcgcc tgtccggttg acgctatcag
    16801 tgcttgatag tagtcaccca cgttggtgcc aatcaccacc tgaaattgcc tggcattggt
    16861 gaaacagcct ttcaccatgg ggagttgctc aatttctttc ggtctggcat tggccggttg
    16921 gttgaggaca aagcgtaggc gagtaataca gtggctcacc gtcgcaatat tgccgcgccc
    16981 gccgaccagt tcaatcaacc gatcgatatc cgtttggttt attttgctca tcataaagcc
    17041 ccatggcaga tgacattttt ggttggctgc agaatatatt gcgcggaaaa atttaaaaac
    17101 gggaacgttc ccgaaacgca gcgaagatca caatttatcg ttcaggaaac gatctggaca
    17161 gggtggcggg gatgatgatt tgttgcggtt cgctgcgccc ggttacctgt gcgatcaact
    17221 ggcaggccgc ctggcgtcca gcttcggcgt aaccgggatc tacggtgacg atctccggat
    17281 ggaggaattt cattaacggc gtattaccga cgctcgccag ttgcaaggtg tcgatgcgtt
    17341 gctcttgcag gtatttactt gcgccaagtg ccagcgtgtc ggttgcgcac agtaaggcgg
    17401 tagtttcagg cgtaatcact tttgctacgt tctcatagcc ttgcttcata gcaaggcctg
    17461 gcagagcggc aacgggatgc agtttatgcg ctttgcagaa cgccaggtag gcttcgtgac
    17521 gtcgcttacc ggtcgtcacg tcactgtgcg gcacgccgag ataactgata ttacgatgcc
    17581 cctggtcata cagccgttgc atcaggatct tgattgcccc ttcgtcgtca taacagaccg
    17641 aagcaaagcc ttttgcgtca cgcgccagca gaaccagcga tgactgccag tgggctaaca
    17701 tttcttctgt tatgccggta aaaccgaaca gcactacgcc gtcgatatta cgccgtttca
    17761 gcacccccaa atgttcggca actaattgcg gggaaaactg actttccatc atgattgggt
    17821 cgtaaccttg ttcatagaac tctggcagca tggtttgaac ggcgagattt tctgacaacg
    17881 aatccaggcg ggtaacaatg atggcgacca ctttatcgct ttgcccacgc atagcgcgcg
    17941 cagagcggga aggggaaaat ccatgctgat tcatcactgc ttcaacacgc tcgcgggtgc
    18001 gctggctcac gccgctttcg ttattcagca cccgggaaac tgtagatttc cccacgccgc
    18061 ttaagcgcgc gatgtctttg atggtcagcc gattttgcat cctgttgtcc tgtaacgtgt
    18121 tgtttaatta tttgagccta actttacccg tgcattcagc aatgggtaaa gtctggttta
    18181 tcgttggttt agttgccagc aggtattata tcgccataga tgctacgaat attattggat
    18241 tctccttatt atttgcggcg cttttttcac ttaccggagg ttatatggaa cctgatccca
    18301 cgcctctccc tcgacggaga ttaaaacttt tccggtaagc ccgtcttttc acggcgttac
    18361 cggatgcgta aggccgtgac gttttaacgt ccctgctcag ctttattacc ttcaggtaag
    18421 gcttcgccac gcctgaagac atttctgtac tgtttcagac agtgcggagg gactccttat
    18481 gtttaaagaa atttttaccc ggctcattcg ccatttacct tcccgtctgg ttcatcgtga
    18541 tccattgcct ggcgcgcagc agacagtgaa tacggtggtc ccgccgtcct taagtgcgca
    18601 ttgcctgaaa atggcggtga tgcccgaaga agaattgtgg aaaacgttcg acacccatcc
    18661 ggaagggtta aatcaggcgg aagtggaatc tgcccgcgaa caacatggtg aaaataaatt
    18721 acccgcacaa caactgtcgc cgtggtgggt acatttatgg gtctgctatc gcaacccctt
    18781 taatatttta ctcaccattc tcggcgctat ttcttacgcc acggaagatt tatttgctgc
    18841 gggcgttatc gcgctaatgg tcgctatttc tacgttgctg aactttattc aggaagcacg
    18901 ttccactaaa gcggcagatg ccctgaaagc gatggtcagc aataccgcga cggtgctgcg
    18961 cgtaattaac gacaaaggcg aaaatggctg gctggagatc ccgatcgacc agctggtgcc
    19021 cggcgatatt ataaaactgg cggcggggga tatgatcccg gcagatttac gtatcttgca
    19081 agcgcgggat ctgttcgtcg ctcaggcgtc gttaaccggt gagtctctgc ccgtggaaaa
    19141 agccgctacc actcgccagc cggagcacag caatccgctg gagtgcgaca cgctgtgttt
    19201 tatgggcacc accgtggtga gcggcacggc acaagcaatg gtgattgcta caggtgccaa
    19261 tacctggttt ggtcaactgg cggggcgtgt tagtgagcag gaaagcgagc cgaatgcctt
    19321 tcagcaaggg atcagccgcg tcagtatgct gctgattcgc tttatgctgg tgatggcacc
    19381 ggtggtgctg ttaatcaatg gctacaccaa aggcgactgg tgggaagcgg cgctgtttgc
    19441 gctttcggtc gcggtaggcc taacgccgga aatgttgccg atgattgtta cctcgacgct
    19501 ggcgcgcggc gcagtaaagc tgtcgaaaca gaaagtgatc gtcaaacatc tggatgctat
    19561 tcagaacttt ggcgcaatgg atattctgtg cactgataaa accggcaccc tgacgcagga
    19621 taaaattgtg ctggagaatc ataccgatat ctccggtaaa accagcgaac gcgtgctgca
    19681 tagcgcgtgg ttgaacagtc attatcagac cggacttaaa aacctgctcg atacagcggt
    19741 gctcgaaggt acggatgaag agtcagcgcg ctcgctggcc agtcgttggc aaaaaatcga
    19801 tgagattccg tttgatttcg agcgtcgccg gatgtcggtg gtagtggcag aaaataccga
    19861 gcaccatcag ctggtttgca aaggtgcatt gcaggaaatc ctcaatgtgt gttcgcaggt
    19921 gcgtcacaat ggcgagattg tgccgctcga tgacatcatg ctgcgtaaga ttaagcgggt
    19981 tactgatacg ctgaatcgtc aggggctgcg cgtggttgcg gtggcgacga aatacctgcc
    20041 agcgcgtgaa ggagattacc agcgggcgga tgaatccgac ctgatcctcg aaggatatat
    20101 tgcttttctt gatccgccaa aagagacaac cgctccggca ctgaaggcat taaaagcgag
    20161 tggaattacc gtaaaaatcc ttactggcga cagtgagtta gttgcagcga aagtgtgcca
    20221 tgaagtgggg ctggacgcgg gagaggtggt cattggtagt gatattgaaa cgctatctga
    20281 cgacgaactg gcaaatcttg cacagcgcac cacgctgttt gcccgcctga cgccgatgca
    20341 taaagaacgc attgtgacct tactgaagcg cgaagggcat gtggttggct ttatgggcga
    20401 tggtattaat gatgcgcccg ccttacgcgc ggcggatatc ggcatttctg tggacggcgc
    20461 ggtagatatt gcccgtgaag cggctgatat catcctgctg gaaaaaagcc tgatggtgct
    20521 ggaagagggg gttattgagg gacgtcgcac tttcgccaac atgctgaaat acatcaaaat
    20581 gacggcgagt tctaactttg gtaatgtgtt cagcgtgctg gtggcgagtg ctttcttgcc
    20641 cttcctgccg atgttgccgt tacacttgct tattcagaac ctgctgtacg atgtgtcaca
    20701 ggtggcgatc ccgtttgata acgtcgacga cgagcaaatt caaaagccgc agcgttggaa
    20761 tccggcggat ctggggcgct ttatgatctt cttcggaccg atcagctcga tcttcgatat
    20821 tctgacgttt tgcctgatgt ggtgggtatt ccatgccaac acgccggaaa cgcaaacgct
    20881 gttccagtcg ggatggtttg tggtgggctt actgtcgcaa acgctgattg tgcatatgat
    20941 ccgcacccgc cgtgtgccgt ttattcagag ctgtgcatcg tggccgttaa tgatcatgac
    21001 cgtgatcgtg atgattgtcg ggatcgcatt gccgttttca ccgctggcca gttatctgca
    21061 attacaggcg ttgccgttaa gctatttccc gtggctggtt gcgattctgg cagggtatat
    21121 gacgttaacc cagttggtga aaggatttta tagccgtcgt tacggctggc agtaaagaat
    21181 aaactaggca tggtggcccg gattcagatt gcagacaaag tgagttttgt tcatgccgga
    21241 tgcggtgtaa acgcctaatc cggcctacaa attcaatcaa ttgcaggaat cacgtaggcc
    21301 tgataagcgt agcgcatcag gcgattttgc gtttgtcatc agacttaatc cgggcatgat
    21361 agcccggatt tccatcaaga ttagcgacga atagcgatcg cttcgatctc aatctttacg
    21421 tctttcggca gacgggcaac ttcaacgcaa gaacgtgccg ggaaggtggc gttgtgttcg
    21481 gtgaagaagg cttcgtaagt ggcgtttacg gttgcgaagt cgttcagatc ttttacaaac
    21541 acggtagttt taacgatgtc gcccactttc agaccagcgg cttcgacgat cgcttttacg
    21601 ttatccagcg actgacgtgc ctgtgcagcg acgtctgccg gtacttcgcc cgttttcgga
    21661 tttaccggga tctgaccgga ggtgatgatc atatttccca gatcaacgcc ctgtacgtaa
    21721 ggaccgatag ctgctggtgc attttccgtc gcgatagttt tgctcatgat ttctccttta
    21781 ttacagcggt aaaaagtctg gctcattata gggagccaga cttttattac caaccgcaat
    21841 taattggcca gcaccacatt atgggaaaac tctttttcac agtatttgca tttgagcgcg
    21901 atgtcattgg cgcgttttcg cacggcaaag ctggatgaaa ccggttcggc atggctgata
    21961 cagttgctgt tcgggcagac caacacattg tcgatgcgct ctggcagact ggggcgcgat
    22021 ttacccacca cttcatagtt gtcgatacgg ttaaccgtgg cttgcggcgc atacaatgcc
    22081 agttgatcta cctgatcttc actcaaaaag gtattttcga ttttgatcag atctttgcgg
    22141 cccatctcgc cagaaggcag gttcagacca atggtgatgc gctgatccgt ttcggtcagc
    22201 ttgaacagac tcaacagctt aaaaccgatc tgggcgggga tatggtcaat taccgtgccg
    22261 cgtttaatag cttcaacctg caatttatta tcgtgtgtca tctctatttc cccttacagt
    22321 accagatcgc gattcagaac cagtgccagt aacgcctggc gagcgaaaat cccgttgcct
    22381 gcctgctgga agtaccaggc gtgtggcgtt ttatcaacat ccgtcgcaat ttcatcaaca
    22441 cgcggcagcg gatgcagtac tttcatattg gctttggcgt tgtggagatc gctggcgcga
    22501 agaacaaact gcgctttcac gttggcgtat tcggatgggt ccaggcgctc tttttgcacg
    22561 cgggtcatat acaggatgtc tacttccgcc atcacttctt caatagagct gtgcagactc
    22621 catgcgatcc ctttttcatc gagcatatcc agaatgtatt gcggcattgc cagcgcgtcc
    22681 ggcgcgatga agtaaaaacg gttgccgtcg aacttcgcta acgcctgagt cagggagtga
    22741 acggtgcggc catatttcag gtcaccaacc attgcgacgt ggagattgtc cagacgcccc
    22801 tgggtttcct gaatagtgaa taagtccagc aaggtttgcg tcggatgttg gttggagcca
    22861 tcaccggcat tcagtaccgg tacattgccg gaaaactcgg tggccaggcg cgccgcacct
    22921 tcctgcggat gacgcatcac tatcgcatcg acgtaagtgc tgataaccga aatggtatcg
    22981 gccagcgttt cgcccttttt acccagtgat gtattggcgc tgtcggagaa gcccaccacg
    23041 ctggcaccca ggcggtgcat ggaagtttcg aaagagaggc gggtacgggt agaggcttcg
    23101 aagaagcagc tggcaatgac tttgtgcttc aacagctctg attgcgggtt tgctttcagt
    23161 tttgccgctg ttgccagcac cagattaagg tcatcgcgac taaggtcgtt tatggaaatg
    23221 atatgtttct gatatagcgg attagccatc ttttatctcc tgacgcctgg gcaaaaaaaa
    23281 gcccctcgat tgaggggctg ggaatgggtg atcaacggga agaaaaacgg caggccagcg
    23341 tcttttttca gacgcggtaa gacaaaatgt cgaacacact gaaccataca tcctcccggc
    23401 aaattgtccg gcattatact catcgtcaga agcggtgcaa gcaattgatg caatattttg
    23461 tcagcgcaaa cggtttattt gaatttaaag ttaaaatata tgcattttta tttactgtga
    23521 ttctgggggg aactgtcgtt cggtatcaat aattgcagac atttcctgct tttttctttt
    23581 tcactttcac gcaatcagat atgcatttta ttccattcat ccgacttata gggcggagtg
    23641 tgaaaacgaa cggctagcac tattgcttag agctcaggga tgcgcgctat cactttaatt
    23701 tcaaaatcaa agcctgccag ccatgtaacg cccaccgccg tccaggttgg ataagatggc
    23761 gcgctaaata tttcattttt caccgtcatg atgtcttcaa attgtttttc tggatcggta
    23821 tgaaagctcg taacatcaat gatatcgtca aaagtgcatc ccgcagctgc cagggtcgca
    23881 tgcaaattat caaaggccag tctgacttgt tgctgaaaat cgggttctgg tgttccgtcc
    23941 tctcgacttc ctacttgccc ggaaacaaac agcaaatcgc cggaacgaat agccgcagaa
    24001 taacgatgct cagcatacag tgaatgtcgt ccagcaggga aaacagcggt tctttctacc
    24061 atttggttat cctcaagatt tacgacatga acagaagatt tatatttacc aggagccgct
    24121 tttagcggac gacgtgagta aacaaaaccc agacatcatg ggaaatggct gggcttaatt
    24181 gagcgtagtc ggttatgcgc caaacgcgcc atcaatggta tgcatcgcgc cggtaacaaa
    24241 actggcttct ggccctgcta accatgcgac cataccagcg acctcttccg gttgcccatg
    24301 tcttttgatg gccataaaac tatgcgacat atcgcgcatt ggcccgttgg cgggattagc
    24361 gtcggtatca attggccctg gctggacgac gttgatggtg atcccacgcg gtccgaaatc
    24421 acgggccagc ccgcgcgcca tgccttgcag ggcagattta ctggcggcat aagcagccat
    24481 gcctgcaaca ggcatacgat cgccattcac ggagccgatg attaagatgc gtccgccttc
    24541 gggcatctgc cgggcggctt caacagaggc atggtaagga gcatgaatat tgattttgaa
    24601 aaggcgatca atatcgtcgg catttaattc cagggcatcg ccaaagacgc caatacctgc
    24661 atttaccacc aggatatcca atgcgccgct cttacgaacg acatcaataa cagcgtcttt
    24721 gtcagcacta tctgtgaata ctgctgtcgc tccagtctct tgtgccaggc gttcagcggc
    24781 atctttcgac cccgcatagg tgaatcgtac attggcccca tcggtgacga aacgacgtac
    24841 gatagcggca ccgattccac ggctaccacc gaggatgaga actgtcttac ctgtaaaagc
    24901 gcccataagg actccttgat ttattatgta acatgcatta caaaactgtt ttaactttct
    24961 gtcaacaagt tttgtaatgg tcactaaaaa acaatctcgc gttccaggtc gtcccagacg
    25021 tttcgctcct gagcaggcag tctctgcggc aaaagtgctt tttcaccaaa aaggtttcga
    25081 tgctgtcagt gttgctgaag ttactgatta tcttggtatt aaccccccga gcctctacgc
    25141 ggcttttggc aataaggctg ggttatttag ccgtgtactc aatgaatatg tcggtacgga
    25201 agctattccg cttgccgata ttcttcgtga tgatcgtccg gtaggggagt gcctggctga
    25261 ggtattaaaa gaagcggcgc gccgatatag ccaaaacggc ggctgcgctg gctgtatggt
    25321 tcttgaaggc attcatagtc atgatccaca agcgcgtgat attgccgttc aatattatca
    25381 cgccgcagaa acgaccattt atgactatat cgccaggcgg catccacaaa gcgcacaatg
    25441 tgtgactgat tttatgagta ccgtgatgtc agggctttct gcgaaggcac gggaggggca
    25501 ctccatcgaa caactctgtg caacagctgc actggcgggg gaagcgataa aaactattct
    25561 caaggagtga tgcgggcctt gacccgaaag gcgggaacgc gcctgccgat aagttgtgat
    25621 aagacaataa ttcacgcgtt aaggctagcg gaattgatca tcttttcgta taacgatgga
    25681 aatgaaacgt tgttttaatt aaggagtgga aaagatgatc atcggaaata ttcataatct
    25741 tcagccgtgg ctaccccagg agttacgcca ggcgattgag catatcaaag cacacgttac
    25801 ggcagaaacg ccaaagggca agcacgatat cgaaggcaat cgtctgtttt atcttatctc
    25861 ggaagatatg accgagccgc acgaagctcg ccgtgcggag taccatgccc gctatctcga
    25921 cattcagatt gtgttaaaag gtcaggaagg catgaccttc agcacgcaac ctgcaggcac
    25981 cccggatacc gactggttag ctgataaaga catcgcattt ttgccggaag gcgttgatga
    26041 gaaaacagtt atccttaatg aaggtgattt tgttgtgttt tatccggggg aagtgcataa
    26101 accgctgtgc gcagtgggcg cgccagcccg ggttcgcaaa gcagtagtga agatgctgat
    26161 ggcgtgatga cttttcgccg taaataactc taggtttacg gcgagtttgt gaaaagagcg
    26221 ttttttgata tttttttgtg agtaaaattt gtaatgctta gacgttctta ttaactcaag
    26281 gagttcgtca tgagcaaaat atcagatttg aattattctc aacacattac attagccgac
    26341 aattttaaac aaaaaagtga agttttaaat acctggcgtg ttggaatgaa taattttgcc
    26401 cgtaatgccg aggggcagga taacacaaga aatatccttg atcctaagac atttttggaa
    26461 tttttggtaa aaatatttac tctaggttat gtggatttta gcaaacgctc caacgaagcg
    26521 ggaagaaata tgatggctca tattgagtcc tcatcttata tcaaaaataa tgatggcagt
    26581 gagataataa agtttgttat gaataatcct gaaggggaac gagcggattt atctaaggtg
    26641 gaaatagaaa tcacactttc cactttcact actatgggga ctcgtcaagg acatacggct
    26701 attatatttc aacaacctga tggttcgact aatcgttatg aaggaaagtc ctttgaaaga
    26761 aaagatgaga gttcattaca cctgattact aacaagattc tggcatgtta ccaacgcgaa
    26821 gctaacaagg aaatagctcg tctattaaat atttcacagg agttaaataa ttctcaggac
    26881 ttgaataact cgcaggtgag ttgtaaagat tcagttgatt ctacgattac ggatttatta
    26941 gaaaaaccat tgaataatgc attattagca ataaggaaag aacatctgct attaatgcct
    27001 tatgtatgta atgaatcgat ttcatacttg cttggtgaaa aagggatact gaaagaaata
    27061 gatgacctta acgcagtaaa taattatctt cttaataata agaaagctac tgacaatgaa
    27121 attaatgaca tcaaagttaa cctgtctcat attcttattg
//
LOCUS       DAJVTY010000040        27018 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVTY010000040 DAJVTY010000000
VERSION     DAJVTY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27018
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.040"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1150)
                     /gene="eno"
                     /locus_tag="OQQ66_001531"
     CDS             complement(<1..1150)
                     /gene="eno"
                     /locus_tag="OQQ66_001531"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1904504.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADL"
     gene            complement(1238..2875)
                     /gene="pyrG"
                     /locus_tag="OQQ66_001532"
     CDS             complement(1238..2875)
                     /gene="pyrG"
                     /locus_tag="OQQ66_001532"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1904505.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3103..3894)
                     /gene="mazG"
                     /locus_tag="OQQ66_001533"
     CDS             complement(3103..3894)
                     /gene="mazG"
                     /locus_tag="OQQ66_001533"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1904506.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(3965..4300)
                     /gene="mazF"
                     /locus_tag="OQQ66_001534"
     CDS             complement(3965..4300)
                     /gene="mazF"
                     /locus_tag="OQQ66_001534"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS1904507.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4300..4548)
                     /gene="mazE"
                     /locus_tag="OQQ66_001535"
     CDS             complement(4300..4548)
                     /gene="mazE"
                     /locus_tag="OQQ66_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS1904508.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4626..6860)
                     /locus_tag="OQQ66_001536"
     CDS             complement(4626..6860)
                     /locus_tag="OQQ66_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1904509.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6908..8209)
                     /gene="rlmD"
                     /locus_tag="OQQ66_001537"
     CDS             complement(6908..8209)
                     /gene="rlmD"
                     /locus_tag="OQQ66_001537"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1904510.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8266..11022
                     /gene="barA"
                     /locus_tag="OQQ66_001538"
     CDS             8266..11022
                     /gene="barA"
                     /locus_tag="OQQ66_001538"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1904511.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11254..12594)
                     /gene="gudD"
                     /locus_tag="OQQ66_001539"
     CDS             complement(11254..12594)
                     /gene="gudD"
                     /locus_tag="OQQ66_001539"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1904512.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWLLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12615..13955)
                     /locus_tag="OQQ66_001540"
     CDS             complement(12615..13955)
                     /locus_tag="OQQ66_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1904513.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13957..15327)
                     /gene="gudP"
                     /locus_tag="OQQ66_001541"
     CDS             complement(13957..15327)
                     /gene="gudP"
                     /locus_tag="OQQ66_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1904514.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15744..16193)
                     /locus_tag="OQQ66_001542"
     CDS             complement(15744..16193)
                     /locus_tag="OQQ66_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1904515.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16211..16993)
                     /gene="truC"
                     /locus_tag="OQQ66_001543"
     CDS             complement(16211..16993)
                     /gene="truC"
                     /locus_tag="OQQ66_001543"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1904516.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16993..17322)
                     /locus_tag="OQQ66_001544"
     CDS             complement(16993..17322)
                     /locus_tag="OQQ66_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1904517.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(17944..18489)
                     /gene="syd"
                     /locus_tag="OQQ66_001545"
     CDS             complement(17944..18489)
                     /gene="syd"
                     /locus_tag="OQQ66_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1904518.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18557..19405
                     /gene="queF"
                     /locus_tag="OQQ66_001546"
     CDS             18557..19405
                     /gene="queF"
                     /locus_tag="OQQ66_001546"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1904519.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19517..20881
                     /gene="ppnN"
                     /locus_tag="OQQ66_001547"
                     /gene_synonym="ygdH"
     CDS             19517..20881
                     /gene="ppnN"
                     /locus_tag="OQQ66_001547"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1904520.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21438..22727
                     /gene="sdaC"
                     /locus_tag="OQQ66_001548"
     CDS             21438..22727
                     /gene="sdaC"
                     /locus_tag="OQQ66_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1904521.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22785..24152
                     /gene="sdaB"
                     /locus_tag="OQQ66_001549"
     CDS             22785..24152
                     /gene="sdaB"
                     /locus_tag="OQQ66_001549"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1904522.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24264..25019
                     /gene="xni"
                     /locus_tag="OQQ66_001550"
     CDS             24264..25019
                     /gene="xni"
                     /locus_tag="OQQ66_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1904523.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25124..26261)
                     /gene="fucO"
                     /locus_tag="OQQ66_001551"
                     /pseudo
     CDS             complement(25124..26261)
                     /gene="fucO"
                     /locus_tag="OQQ66_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26289..26936)
                     /gene="fucA"
                     /locus_tag="OQQ66_001552"
     CDS             complement(26289..26936)
                     /gene="fucA"
                     /locus_tag="OQQ66_001552"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1904524.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6538 a         7251 c         6510 g         6719 t
ORIGIN      
        1 ccaggtcagc gatggtagcg tcttcagttt cgccagaacg gtgagagata actgcagtgt
       61 agccagcatc tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt
      121 tgaatttgat caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg
      181 tgttggttac gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggtct
      241 ggtatgcgaa accgtcccag tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt
      301 actgtttggt cagttcttcc aggaagtgag tgaattcttc agaggtgaac gctttgttgc
      361 cttcgccagc cagaacgtat ttaccatctt tgtagaattc agaagctgcg cagtccatcg
      421 ccagagtgat gtctttgccc agttcataac cagcagcttt aacagcttca gcgataacag
      481 ccagagcttc agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaacagcag
      541 tattcatgcc tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc
      601 ggatggcttc tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa
      661 cgttgttgtc agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatagagt
      721 atttgcccgg agtaccgttc agttcagcga tgtgctcgta cagcggcata cctttagcag
      781 ctgcagcagc tttggcgtta gccagagata cagccaggat tgcgttcgcg ccgaatttgg
      841 atttgttttc ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttag
      901 catctttgcc aatcagcgcc tgagcgatcg ggccgtttac cgcagcaaca gctttggtta
      961 cgcctttacc caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac
     1021 cagtagaagc acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt
     1081 cggcttcaac agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga
     1141 ttttggacat taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacac
     1201 gtacccaggg tacgcgttgc cgttctaact tttttactta cttcgcctga cgtttctgga
     1261 actcgctggc ggctttcaca aagcctgcaa acagcgggtg accatcacgt ggagtagaag
     1321 taaactccgg atggaactgg caagccacga accacgggtg attcggaact tcgatgatct
     1381 cgaccaactg atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt
     1441 tcaacagcat gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat
     1501 tgtacagctg gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca
     1561 tggtaccgcc gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc
     1621 actcggtaat cagcgccaca accgggtact tacagtctgg cacaaattcc gtagagttgg
     1681 cgttctccat gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc
     1741 aaatgcccag ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt
     1801 ctacgccacg atagccgaaa ccgccaggta cgaggattgc gtccagacct ttaaggattt
     1861 caacgccgcg cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat
     1921 tcttcagccc accgtgtttc agtgcttcga tcactgattt ataagcatcc ggcagttcaa
     1981 tgtacttgcc gaccataccg atggtgactt cacttaccgg gttcgcttct tcgaagataa
     2041 cctgttccca ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat
     2101 aatcgtccag cccctgagat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt
     2161 tcagagaaat aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt
     2221 tcgccggaac agcgcgatct gaacgacaaa tcaggatgtc aggctggata ccgatggaga
     2281 gcagctcttt tacagagtgc tgagtcggtt tggttttgac ttcaccagac gctgccatgt
     2341 acggcaccag cgtcaggtgc ataaacagag tgtgctcacg gccaatttca acagccatct
     2401 ggcgaatcgc ttcgaggaac ggcaaggatt cgatatcacc tactgtaccg ccgatttcta
     2461 ccagtactac gtcatgacct tcgccacctt ccagcacgcg ctctttgatt gcgttagtga
     2521 tgtgcggaat aacctgcacg gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa
     2581 cgtcagagta gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga
     2641 aacgctcgta gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt
     2701 ccccgtgttg gattgggctc atagtacctg gatcgacgtt gatgtacgga tccagtttca
     2761 tgatggtcac attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc
     2821 ctttacccag agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga
     2881 acctgagaag ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg
     2941 aacatgacct gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat
     3001 caggagtgag aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc
     3061 atttaaaaaa tgacacaaat ggcacttgac cgcgtaattc ccttagagat caatttcctg
     3121 ccgttttacc tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag
     3181 tccacgcgcg gcaacaatac gctccacttc gcgaaaacga cgctcgaatt tttcgttcgc
     3241 tttttgcaat gcgatttctg ctttcgtccc taaatggcga gccagattaa ccgtggcaaa
     3301 cagcaggtcc cccatttcct cctccagttt agcctggtcg acaacagcct gccgcgcttc
     3361 gtacatcacc tcgtcgatct cttcgtagac tttatcaacc accgggccaa gcgtcgtcca
     3421 gtcgaagcca acgttggcgc aacgtttctg gattttttgc gcccgcatta aagccggtaa
     3481 actacgagga atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt
     3541 gatttgctcc caacgggcaa gcacttcact actgttttcg gcagaactat cagcaaaaac
     3601 atgcggatgg cgacgctcta atttatcgct aatagcagcg caaatatcat taaagtcaaa
     3661 gcgcccttct tcctgagcca tttgcgcgta aaacaccacc tggaatagca gatcgcccag
     3721 ttcaccgcga agatcgtcaa aatcttcacg ggcgatggcg tccagcactt cgtaggtttc
     3781 ttcaagggtg taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt
     3841 ttccggatcg cgcaggcgct gcataatagt gagcaaacga tcgatttgat tcattgaatt
     3901 gtcctgaaaa ttgcgggtct gtcaggtgga aacctgtgac cagaatagaa gtgagttagt
     3961 aacactaccc aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa
     4021 ctgttccttt cttcgttgct cctcttgccc gccaggcgat tctttttacc tgatcagcta
     4081 acgctacgcc atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt
     4141 gcgttgtaca aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca
     4201 ggacaacagc tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc
     4261 aaatcagatc gcccatatcg ggtacgtatc ggcttaccat taccagactt ccttatcttt
     4321 cggctctccc cagtcgatat tctcgtggag gttttccggc gtgatgtcgt tgaccagttc
     4381 agcaagcgta aatacgggct ctttacgcac tggctcaata attaatttgc catccaccag
     4441 gtcaatcttc acttcatcat caatattcag attgagcgcc tgcattaacg tagccgggat
     4501 ccgcaccgcc ggtgaatttc cccaacgctt tacgctactg tggatcatca ccctttcctc
     4561 aaaccgctat catatgtaga tacagtatat atcaatctac attgtagata cgagcaaatt
     4621 tctgcctaac tcccgtgcaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta
     4681 cccagcacgc ggcccagcac ctgcaggttg taaatctcaa tggtcatgtc gatggtcgcc
     4741 agttgctgtt tggtgtcgct acggctggca acgccaagta cgttcacctt ctcgttggcg
     4801 agaatggtcg tgatatcacg taacaaccca ctacgatcat tagccaccac gcggaccacc
     4861 agcgaatatc cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca
     4921 tgagagcgca gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgcccc
     4981 tgggtaatga agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg
     5041 tgcatcaggt tgccaacacc ttcgactacc acgcgaccgt tatctttact gcggttttgc
     5101 ggcgtgtagc ttttttgctg aagttgcttc agcgcggcgg cgtcctgctc ttcggcactc
     5161 ggcttattaa attgcgattg caggaagttc accatctgat tgagacggat atccccgcca
     5221 ccaatcgccg ccagcaactc gtcgacatca ttaaagttgt aacgcggcag cagatgtttt
     5281 tctgcttctt tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca
     5341 gccagaatgt ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca
     5401 cggctggttg tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt
     5461 ttctgggtga taatttcaat ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg
     5521 cgcccgccaa tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg
     5581 aagtccagcg gcgttgatcc cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca
     5641 tacacccggt cgtcaaagac ctggctgcgt acttcgtcga gcatttcgcc ggaatcagcc
     5701 atctcttcct gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa
     5761 cgtgcgccgc cagctgccgc gccctcttta tatttccagt gcgcagcaac acccaactct
     5821 gcatcttcat gcatctgttt ggtgcggatt tggatctcaa cggttttgcc acctggcccc
     5881 agaaccacgg tatgaataga ctgataaccg tttggtttcg ggttagcgac ataatcgtca
     5941 aactcatccg gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc
     6001 tgtaaacgct cggcgacaat acgtaccgca cgcacatcaa acagctcatc gaaggcgagg
     6061 ttctttttct gcattttgcg ccagatgctg tagatgtgtt tcggacggcc atacacttca
     6121 gctttaacgc cttcagcttt catctcagcg cgcagatgac cgacaaactc ttcgatatag
     6181 tgttcgcggt cgagacggcg ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg
     6241 tgcagataac ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg
     6301 ttcgccaacg gcgcgtagat attggtgcac tcttttgccg ccagtacgcg ttcatcttcc
     6361 ggcgcatctt ttacttcgcg cagatgagca atacgctccg ccagtttgat gaccacgcag
     6421 cgaaaatcat cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa
     6481 acagaatcag tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga
     6541 ataaggttaa cgacagactt accgacgctc tcacgcagca catcttcgct gactacgttg
     6601 gcatcggcca gagggaacag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc
     6661 gagaggatct ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc
     6721 tgcgtctgct gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg
     6781 gtaataccca gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt
     6841 gcacttctta ccgcaaccat tgtcctctcc tttagggacc agacctgccg aaatcggcaa
     6901 atcgtaacta tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga
     6961 acatatccag catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat
     7021 cccgagccag cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta
     7081 taatttgctg cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga
     7141 agccgttttt cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg
     7201 tcacattctg taagccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa
     7261 caccttctac accgaccaca ctggcagctt gtgtcgccaa tggcagtgta aagttgccca
     7321 taccgcagaa cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac
     7381 tcgctaccat tttttggttc acacccgcat tgacctgaat aaaatcgcgc gggctaaaag
     7441 ttaagcgcaa cccgttagag tcataccagg gcatctcacc agagacggtt tcgagtatct
     7501 cactatcggg ggcgagatac agatccaggc cttcagaatg cgaaaagcgt tccagttttt
     7561 cgcgatctgc cgaacttagc ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg
     7621 cctgtaccag ttcaacatga ccaagatggc gcattgcttg taagctgccc aggcatgccc
     7681 tgactttggg cagcaatgct tcaagttggg gcactaaaat gggacattgt ttaacgtcga
     7741 caatgtcact ggagcccgct ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt
     7801 agttcagact taaacgcgcg cgacggcgat agccccaggg aacatcggcg atcacttcag
     7861 agacttcgtg tttcattaat cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca
     7921 cgctggcgtg ttgttgctga cagccgccgc atacgccaaa atgaggacag cgtggcgttt
     7981 cgcgttccgg gctatcgctt aaccggcgta cgactttagc gcgggcatac tgttttttat
     8041 cttcagtaac agtaacttcc gcgttttcct gcggcaataa tccggggata aatagcgttt
     8101 tgccgttatg tcgcgccacg ccctgaccaa aagagtcgag gtcgttgact gaaacggtta
     8161 tgatctgacg cgtcgtcgtg cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt
     8221 tctcaattta acagtgtgac cttaattgtc acataacgga actccatgac caactacagc
     8281 ctgcgcgcac gcatgatgat tctgatcctg gcaccgaccg tccttattgg tttattgctg
     8341 agtatatttt tcgtcgtgca tcgctataac gacttgcagc gtcaactgga agatgcaggt
     8401 gccagcatta ttgagccgct tgcagtttct actgaatatg gcatgagcct gcaaaatcgc
     8461 gaatctatcg gtcagttaat aagcgtactg catcgtcgcc attccgatat tgttcgcgcg
     8521 atttcggttt atgatgaaaa taaccgactc tttgtcacct ccaattttca tcttgatccc
     8581 tcatcaatgc agctcggcag caacgtgccg tttcctcgcc agctcactgt cactcgtgac
     8641 ggcgatatta tgatcctccg cacgccgatt atttctgaga gttactcccc cgacgaatcg
     8701 cccagtagcg atgccaaaaa tagtcaaaat atgttgggat atattgcgct tgagctggat
     8761 cttaaatcgg ttcgcttgca gcaatataaa gagatcttta tttccagcgt gatgatgctg
     8821 ttttgtatcg gtattgcgct tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg
     8881 attcgcaaca tggtgaatac cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg
     8941 gaaggattta tgctcggcga gctggatatg ctgaaaaacg gtatcaactc gatggcaatg
     9001 tcgctggctg cttatcacga agagatgcag cacaatatcg accaggcgac gtccgatctg
     9061 cgtgaaacgc tggagcagat ggaaattcag aacgttgagt tggatctggc gaaaaagcgc
     9121 gcccaggaag cggcgcgtat taaatccgag tttctggcaa atatgtcaca cgagctgcgt
     9181 acaccactga atggtgttat tggctttacc cgcctgacgc tgaaaacaga attaacacca
     9241 acgcagcgcg atcacctgaa tacgattgaa cgttcggcaa ataatttgct ggcaattatt
     9301 aatgatgttc tcgacttctc gaaactggaa gcaggtaagc tgattctgga aagtattcca
     9361 ttcccactac gcagcacgct ggatgaagtc gttactctgc tggcacattc ttctcacgat
     9421 aaagggttgg aactgacgct caatattaaa agcgacgtgc ctgataacgt gatcggcgac
     9481 ccactgcgat tacagcaaat catcactaac ctggtgggga atgcaattaa attcaccgag
     9541 aatggcaaca ttgatattct ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt
     9601 gaagtgcaga ttcgggatac cggcattggt attcctgaac gcgatcaatc gcgcttattc
     9661 caggccttcc gacaggctga tgccagtatt tcccgccgtc atggtggcac cgggctgggg
     9721 ctggtgatta cacaaaaact ggttaatgaa atgggcggcg atatttcgtt ccatagccag
     9781 ccgaatcgcg gttcaacttt ctggttccac attaatctcg atctgaaccc gaacattatt
     9841 atcgaagggc catccaccca gtgcctcgca ggtaagcgcc tggcctatgt cgaaccaaac
     9901 tccgcagcag cgcaatgcac gctggatatt ttaagtgaaa cgccgctgga agtggtttat
     9961 agcccaacgt tctccgcgct gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg
    10021 gtgaccttcc gcgagccgct aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg
    10081 accgatttcc tgatgctggc acttccttgc catgcacaag tcaatgctga aaaactcaag
    10141 caagatggta tcggcgcgtg tctgctgaaa ccattaacac ctacacgcct gttgcctgcc
    10201 ctgacggaat tttgtcatca caaacaaaac acgcttttgc ctgtaaccga tgaaagtaag
    10261 ctggcaatga cagtcatggc ggttgatgac aaccccgcta acctgaaact tatcggcgca
    10321 ttgctggaag atatggtgca acatgtggaa ctttgcgata gcgggcatca ggcggttgaa
    10381 cgggcgaaac agatgccgtt cgatttgatc ttaatggata ttcaaatgcc ggacatggat
    10441 ggcattcggg cctgcgaact catccaccag ctcccccatc agcaacaaac gccggttatc
    10501 gcggtaacgg cgcatgcaat ggccgggcaa aaagagaagc tgcttggcgc agggatgagc
    10561 gattatctgg cgaaaccgat tgaagaagag cgattgcata atttgttgtt gcgctacaag
    10621 cctggcagcg gtatttcctc tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac
    10681 ccgaatgcga ccctcgactg gcaactggca ctacgccagg cagcaggaaa aaccgattta
    10741 gcgcgcgata tgctgcaaat gttactcgat ttcctgcctg aagttcgcaa caaagttgag
    10801 gaacagctgg ttggagaaaa cccggaaggc ctggttgatt tgattcataa actgcatggc
    10861 agttgcggct atagcggtgt gccgcgtatg aagaatctct gccaactgat cgaacaacag
    10921 ctacgtagcg gtactaaaga agaagatttg gagccggagc tgctggaact gttggacgag
    10981 atggataatg tcgcgcgcga agccagcaaa attctcgggt aacggacgtc ttgtcgagta
    11041 gcaatcggct ttttatgacg ctggtttcag attggtgaca aagtgcctgt gtttatgccg
    11101 gatgcggcgt taacgcctta tccggcctac aaaaccaatt aaattcaatg aattaaaaaa
    11161 tgatgtagac ctgatacata gcacaacagg ctattttgcg tttagcatca gtctcaagcc
    11221 ggctccagat agagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt
    11281 atcgaacgtc cagccaggaa tcagatactg cattcccatc gcattgtcac gcgcgcaaag
    11341 cccgtgtttc tgatacagct catgggcttt catcacttga tccatatcga tttctacacc
    11401 cagccccggt ttttccggca cctgaaccag accgcctttg atctcaaacg gttctttggt
    11461 caagcgctga ttgccttcct gccaaatcca gtgcgtatca atcgcggtga ttttacccgg
    11521 tgcagcggcg gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgtttgagtg
    11581 tgaaccccag gtcaggccaa attcatggca catttgcgcc acacgtaccg aaccttgcat
    11641 cgtccagaaa tgcggatccg ccagcgggat atcaacggat tgcagggaga gcgtatggcc
    11701 catctgccgc cagtcggtgg cgatcatatt ggttgcggtc ggcaggccag ttgcgcgacg
    11761 gaactctgcc atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc
    11821 ataagccagc gaccctttca ggtatttacc aattttaatc gcttcgttca gcaaccaggc
    11881 accgttagga tcgagcgtaa tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga
    11941 ctcggcctct tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt tgaagccata
    12001 tttttcatac gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg
    12061 acgcaggcga taccagtcgc atgagtcatc cggctggctt tgatacggca gcggtgtggc
    12121 tttgcgatca ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc
    12181 atcgcccagc agggatgcca cgtttacccc cagatgctgc cccagcagat ccagcattgc
    12241 cgcttctatc ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg
    12301 cccaccagca tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt ttttgtattc
    12361 acccagcgtt ttacctacca ccagcggaat cgcatcttcc agcgttttac ggattttctc
    12421 gccgccggga atttccccta cgccagtgtg accagaatta tctttgataa tcataatatt
    12481 acgcgtaaag aacggtgcgt gtgcaccact cagattcatc agcatactgt catgacccgc
    12541 caccgggatg acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat
    12601 gtcctttttc agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg
    12661 gggatcaggt actgcatcgg acctgcgtcg ttacgcgcac cgccaggcag acgtttatag
    12721 gcctcatgtg ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca
    12781 ggaacggcaa tttttccgtt tttaatctcc agcggatttt tggtcaggcg acaatcgccc
    12841 tcctgccaaa tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca
    12901 tgggtaaaca tcgccagaga gatatcgaaa tggttattag aatggcagcc ccaggtcagc
    12961 ccccagtcgt cgcaaagctg cgccacacgg actgcaccgg aaagcgtcca gaagtgcgga
    13021 tcggcaagtg gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg
    13081 gtggcgatca tgttagtcgc gacgggcaag ccggtcgccc gccgaaactc cgccataact
    13141 tcacgaccgg agaaaccctg ttctgcgccg catggatctt cggcataggt aagaacatca
    13201 ttcagccctt tgcatagaga aatggcttca tcaagtagcc aggcaccgtt gggatcaacg
    13261 gtaatccgcg catcagggaa gcgtttcttc aatgcacgaa cagtgtcgat ttcttgctcg
    13321 ccaggtaaca ccccgccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct
    13381 tccgccagac gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac
    13441 tcatggttgc ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcaccgata
    13501 taaaacagat aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc caacagttcg
    13561 cagaccggaa cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc
    13621 acggcattaa cgtgcaattc aaaagtccag gcacctttgc cgaaggtatc aaaatcggct
    13681 gcctgattac ctttatgcac ctgctgaacc actttattca ggcgcgcaac ttcctggccc
    13741 agaaccatcg gaatagcatc gaccagtgtt tgataaatca cctctccgcc cggcgcttca
    13801 ccaatgccgg tatgcccggc gttatcggtg agtaccacaa tattgcgggt gaaatatgcg
    13861 ttatgtgccc caccaatatt aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc
    13921 atatcagtaa taacaggact ggattgtgtc gtcatcttat tgccccgcga caggtttcag
    13981 ctcgatacgc ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttaaggcatg
    14041 aacaccaaca taaatcagcg ccccattaaa ggagccagtc gtgccaacga tataaccaat
    14101 tgcgattggc gtgacgatgc cagaaatgtt accgaacatg ttgaacaggc caccggaaag
    14161 accgctgatc tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc
    14221 cttaccgaag aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt
    14281 gcagaacacc atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat
    14341 gttcagcgat cccgtgcggc gcatcagcca gtcggaaata atcccaccca gcacaccgcc
    14401 gataaaaccg caaaccgccg gaacggaagc cacaaagccc gctttcagaa ttgacatccc
    14461 acgcgcctgc accagataaa ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat
    14521 acagtactga ccgatataaa cgccgatcat catccgtgac ccgagcagtt gtttgatctg
    14581 cccccacttc acgctgaacg gcactttaac tttggtgttt tgctgatcca tattgatcag
    14641 cgcgccaccc gcggcgatgt actccagctc tttctgattt acccccggat gttgatttgg
    14701 ctcgtggatg actttcaacc agataaagct gatgacaatc cccagaccgc ccataaagaa
    14761 gaagacgtgt gaccagccca cttcatgcgt cagccagccc ataatcggcg cgaagatcac
    14821 cgttgcgaag tattgagcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg
    14881 aaaccaagcc gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac
    14941 caggaagcgc agagtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa
    15001 gccttgcagc aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga
    15061 accaaaacga tccagcaacc aaccaccagg gatctgcccg ataacataag cccatgagaa
    15121 ggcagagaac acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga
    15181 accggcaata gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat
    15241 caccactatc cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa
    15301 agaactcgtt gttgcactcc tgaaaattcg cgttagccac gctaactctg gactgcgaca
    15361 tagccaggaa atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg
    15421 aatacggcgc gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac
    15481 cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga ttttggcatt agccctggac
    15541 ggtggaatcc acttcacgga aatgaaaaca agaacaagaa aggaagggtt aaaacgaaga
    15601 aataaagaag agtatgaaat ggatcgcttg actccaggca aacgccagta aaaatccgcg
    15661 ctatgaagca gtttttactg gcatttgcct gaaaagattc gattcagcac cgctaaaacg
    15721 acatttaccg ctcgctgaac atatcaggac aacagcgtgc cccactgttc gacccacgga
    15781 tttgattccg tttccggttc cgggttctca ctggcgtcaa tcaacagcat ttcgccaact
    15841 cgctgagcgc tctgttcctg caacaaggca tcgaactgtt tgccgccatt gcagaaattt
    15901 acataactgc tgtcgccgag cgcaatcacg ccataacgca gattcggctg gaagcccaga
    15961 ctatctttga ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc
    16021 gtggacgtaa ccaccagaac atacttatcc tgataaggca gccagtcgct taattcagga
    16081 tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa ttgcttctgc ttcttccgcc
    16141 actaacagcg aatttccgta catggtgccg acaaaaatac caatttccgc cataccgttt
    16201 tctccctgga ttaggaactt atctctccat cctgacccga tggcgcactg aactcaaccc
    16261 tttcattttc aggaagcaga ccgcgccagc caaattgtga taacgcctgc atccaggtgt
    16321 cgtccaggcc cgcgtgaata gtcagcggct cgccagtaaa aggatgcgtc aatgacaact
    16381 gactggcatg tagcattaac cgctggaggc caaaatgctc agcaccgctg cgattctggc
    16441 gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg caaatgggca agatgtcggc
    16501 gaagctgatg tttgcgtccg gttttcggct ccagttccac caggccgtag cgcgtggtcg
    16561 ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag accgcgataa tgcgtcactg
    16621 ctggctgcgg gcctttatct tcgcgggcaa atttatcagc gattttgtcc agttcttcca
    16681 ccagtggata atccagcacc gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac
    16741 gtttctggat ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc tcgctggata
    16801 atcccatcaa caacacacca gaagtgggtc ggtccagacg atgagcagta aaaacatgct
    16861 gtcctatctg gttacgcacg gtttgcatga ccactacttt ctcgtcgcga tccagccagc
    16921 tacggtgaac cagccagccg gagggtttat ttaccgaaac cagccattca tcctgataga
    16981 gtatttccag cattagctcg catcatccgc aaaaagagca tccagttttt ccaactcagc
    17041 cagaataagc gcgtgttgcg gatggtccgt cgccagcgcc atttcataat agggtgcaac
    17101 agcaaaagcg cccggtaacg gctggttgtt atttaacaaa tcgtgcattc gcgggatcag
    17161 cacccactgc aaccactcca gtggttccat ggtgtccata aagaacggtt gggtactatt
    17221 aaattgatgc ggctggggtt catcgtttcg ccagtgctga tgttcacgca gtaacgcttc
    17281 aagcgcctgc aactggagac gaacgcggtc atgagtggtc ataaagcaac ctcaataaga
    17341 aaaactgccg cgaaggatag caggaataaa aaaagggagc actgtattca cagcgctccc
    17401 ggttcgtttc gcagcattcc agctactttt gttgctccct gctcatcctt gacaactttt
    17461 cctctggcct tgcggccaat cgttcatcct gaactattgc ttcctgctca caccaccccg
    17521 atgtgatact tcatcctgaa gtgtccttgg ccttcctgac cagccgaatc atcctgaccg
    17581 gttctcattc tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc
    17641 atccagaagc ctttccctga aacaccatcc tggtgtgtcc tgcagaagtg tcatcatcct
    17701 gatgttcact tcgttgtctg actccctgtc gacgaagata gaatcgtctt tttcgcgaag
    17761 tcttacaagg cgcttacaga caatgcctta aggaaaattt catatgaaag ttaagaatta
    17821 acaatcaaca ttttgatttt aaatgaaaat aacgattctt atctttttaa gtattcgctt
    17881 atttcctggc gatctcctac agagtctgta agagatctct cacaaccaga tggggtattt
    17941 ggattacaga agaggcttaa gttgattaag gaattccgca agatttgagg ctaaatgcgt
    18001 ccgtttacgt gttcccagcg tctctttgca cacttcaccg gagagattac acaccgagat
    18061 gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg ggcggcagtt ttagcctttt
    18121 ctgagtcacc agatgaccaa tcagattttc ctgcacccga cggaagtcgt cttcactcca
    18181 ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg tgcatatccc cggcaaactg
    18241 agtcgtataa aaggtatgaa ttgtgggttg tatcacaata tcaaaagcgc gttcaaccgc
    18301 gtttacattt tgttccccgg taaacggctg gggttgccag tatacggcat cttcggtagt
    18361 tgaaataatg cacggtgaag gaacaccgta cagttcctca cttaacggcc agcttttatg
    18421 ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt gtgcggtcaa
    18481 atcgtccact aatttctctc ttcacttaaa ccagatacac ttgtcgttta gtttatctgg
    18541 tttatgacgg tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg gcctgactct
    18601 tggaaaatca accgattacc gggataccta tgacgccagc ctactgcaag gcgttccacg
    18661 cagcctgaat cgcgacccgc tgggtctgaa agcggataac ctgccttttc acggtacgga
    18721 tatctggacg ctgtatgaac tttcctggct gaatgcgaaa ggtttgccgc aggtcgctgt
    18781 cggtcatgtt gaacttgatt acaccagcgt aaatctgatt gagtcgaaga gttttaagct
    18841 ctatctcaac agttttaacc agacgcgttt taataactgg gatgaggtgc gccagacgct
    18901 ggagcgcgac ttaagcactt gcgctcaggg taagattagc gtggcgttat atcgtcttga
    18961 tgaactggaa ggccagccga taggtcattt taatggcact tgcattgatg accaggatat
    19021 cactatcgat aactatgaat tcactactga ctatctggag aatgccacct gtggtgaaaa
    19081 agtagtggaa gagacgcttg tcagccacct gctgaaatca aactgcctga tcacccatca
    19141 accagattgg ggttcgatcc aaattcagta tcgtggacgc caaattgaca gagaaaaact
    19201 gctgcgttac ctggtctcat tccgtcatca caacgagttc cacgaacagt gcgtggaacg
    19261 catctttaat gacctgttac gcttctgcca gccagaaaaa ttgagcgttt acgcacgtta
    19321 tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt aatagcgatt ttgtcccatc
    19381 gaccacaaga ctggttcggc aataaatttt ttctcaattt tgcgtgctgg attcacgcag
    19441 aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa agggaatgac gatattcgtc
    19501 ccataaggag tttttcttga ttacacatat tagcccgctt ggctccatgg atatgttgtc
    19561 gcagctggaa gtggatatgc ttaaacgcac cgccagcagc gacctctatc aactgtttcg
    19621 caactgttca cttgccgtac tgaactccgg tagtttgacc gataacagca aagaattgct
    19681 gtctcgtttt gaaaatttcg atattaacgt cttgcgccgt gaacgcggcg taaagctgga
    19741 actgattaat cccccggaag aggcttttgt cgatgggcga attattcgcg ctttgcaggc
    19801 caacttgttc gcggtcctgc gtgacattct cttcgtttac gggcaaatcc ataacaccgt
    19861 tcgttttccc aacctgaatc tcgacaactc cgtccacatc actaacctgg tcttttccat
    19921 cttgcgtaac gctcgcgcgc tgcatgtggg tgaagcgcca aatatggtgg tctgctgggg
    19981 cggtcactca attaacgaaa acgagtattt gtatgcccgt cgcgtcggaa accagctggg
    20041 cctgcgtgag ctgaatatct gcaccggctg tggtccggga gcgatggaag cgccgatgaa
    20101 aggtgctgcg gtcggacacg cgcagcagcg ttacaaagac agtcgtttta ttggtatgac
    20161 agagccgtcg attatcgccg ctgaaccgcc taacccgctg gtcaacgaat tgatcatcat
    20221 gccagatatc gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggta tcattatctt
    20281 ccctggcggt gtgggtacgg cagaagagtt gctgtatttg ctgggaattt taatgaaccc
    20341 ggccaacaaa gatcaggttt taccattgat cctcaccggc ccgaaagaga gcgccgacta
    20401 cttccgcgta ctggacgagt ttgtcgtgca tacgctgggt gaaaacgcgc gccgccatta
    20461 ccgcatcatc attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat cgatgccgct
    20521 ggtgaaagaa aatcgccgcg acacaggcga tgcctacagc tttaactggt caatgcgcat
    20581 tgcgccagat ttgcaaatgc cgtttgagcc gtctcacgag aatatggcta atctgaagct
    20641 ttacccggat caacccgttg aagtgctagc tgccgacctg cgccgtgcgt tctccggtat
    20701 tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg gtccttacaa
    20761 aatcaacggc gataaagaga ttatgcgtcg tatggacgac ctgctacagg gttttgttgc
    20821 ccagcatcgt atgaagttgc caggctcagc ctacatccct tgctacgaaa tctgcacgta
    20881 atctcccctc ccccggtgac gtttgttgcc gggttttctc gtttttggtc acttactcat
    20941 caactcattt catttgttat atgaatgttt cttaccaccc tcacgcgaca aatatcatca
    21001 cagttaatat gtcatacaat ttatgttgca acgcaaacgt ttccctattt tcataaaccg
    21061 tattttatcg ctatgaaaaa gaaattatcg ccatgattaa ctaaaagtat tgattttttc
    21121 agttcaacct acatatattg cgcgccctgg aagaagtcag atgtcgttta atgggcaaat
    21181 attgccctta aattctcttt tacttttgat ttacagagta aagcgttggg ataatctatc
    21241 ttccaagtag attattgtat ttgagatcaa gatcactgat agatacataa cttgtgtgta
    21301 tctttccgcc ctcaaattat tacggcggta aatgattaag ccatcgccga tagacagatt
    21361 tcatttttac ggtcaggcac cttcccgggc tgaactggct aaaagctgaa ttatttgcat
    21421 tcctccagga gaaatagatg gaaacgactc aaaccagcac gattgcgtcg aaagactctc
    21481 gtagtgcctg gcgcaagaca gacaccatgt ggatgctggg cctttacggc acggcaatcg
    21541 gcgcgggcgt gctgttcctg ccaatcaacg ccggtgttgg cggtatgatc ccgctgatca
    21601 tcatggctat ccttgcgttc ccgatgacgt tttttgctca ccgcggcctg actcgcttcg
    21661 tactgtctgg taaaaacccg ggcgaagaca tcaccgaggt tgtagaagaa cactttggta
    21721 ttggcgcagg taaactgatt accctgctct acttcttcgc tatctacccg atcctgctgg
    21781 tttatagcgt ggcaatcacc aatacagttg aaagcttcat gtctcaccag ctgggtatga
    21841 cgccaccgcc gcgtgcgatt ctgtcgctga tcctgatcgt gggtatgatg accatcgttc
    21901 gcttcggtga gcagatgatc gttaaagcga tgagtattct ggtgttcccg tttgttggcg
    21961 tattgatgct gctggctctg tacctgatcc cgcagtggaa cggcgcagca ctggaaacgc
    22021 tgtctctgga cactgcatct gcaaccggaa acggtctgtg gatgaccctg tggctggcaa
    22081 ttccggtaat ggtgttctcg ttcaaccact ctccgatcat ctcttctttc gccgttgcga
    22141 agcgtgaaga gtacggcgat atggcagaac agaaatgctc gaagatcctg gcattcgcac
    22201 acatcatgat ggtgctgacc gtaatgttct tcgtcttcag ctgtgtactg agcctgactc
    22261 cggcagacct ggctgcggct aaagagcaga acatctcgat tctgtcttac ctggctaacc
    22321 actttaacgc accggttatc gcgtggatgg ctccgattat cgcgattatc gctatcacca
    22381 aatccttcct cggccactac ctgggcgcac gtgaaggctt caacggtatg gtgattaaat
    22441 ctctgcgtgg taaaggtaag tctatcgaaa tcaacaagct gaaccgtatc actgcgctgt
    22501 tcatgctggt aacgacctgg attgttgcca ccctgaaccc gagcatcctg ggtatgattg
    22561 aaaccctggg cggtccaatc atcgcgatga tcctgttcct gatgccgatg tacgcaattc
    22621 agaaagtacc ggcaatgcgt aagtacagcg gtcacatcag caacgtattc gttgtcgtga
    22681 tgggtctgat tgcaatctcc gcaatcttct actctctgtt cagctaagtc ctttcgcgcc
    22741 gctttcgggc ggcgcttcct ccgttttaac gcgatgtatt tcctatgatt agcgtattcg
    22801 atattttcaa aatcggcatt ggcccttcca gttctcatac cgttggacca atgaaagcgg
    22861 gtaaacaatt taccgacgat ctgattgccc gtaacctgct taaagacgtg acccgcgtgg
    22921 tggttgacgt gtacggctcg ctctctctga ccggtaaagg ccaccacact gatatcgcca
    22981 ttattatggg cctggcgggt aacctgccgg ataccgtgga tatcgattcc atccctggtt
    23041 ttattcagga tgtgaatact catggtcgcc tgatgctggc aaacggtcag catgaagtgg
    23101 agttcccggt tgatcagtgc atgaacttcc acgccgacaa cctttctctg catgaaaacg
    23161 gtatgcgcat taccgcgctg gcgggcgata aagtcgttta cagccagact tactactcta
    23221 ttggcggtgg ctttatcgtt gatgaagagc attttggcca gcaggatagc gcaccggttg
    23281 aagttcctta tccgtacagt tcagcagccg atctgcaaaa acattgtcag gaaaccgggc
    23341 tgtcactctc tggcctgatg atgaaaaacg agctagcgct gcacagcaaa gaagagctgg
    23401 aacagcacct ggcgaacgta tgggaagtca tgcgtggcgg tattgagcgc ggtatttcca
    23461 ccgaaggcgt gttgcctggc aaactgcgcg ttccacgccg tgctgcggca ctacgccgga
    23521 tgctggtcag ccaggataaa accaccactg acccgatggc ggttgttgac tggatcaaca
    23581 tgtttgcact ggcagtgaac gaagagaacg ctgctggcgg acgcgtggtg actgcgccga
    23641 ctaacggtgc gtgcgggatt atcccggcag tgctggcgta ctacgacaag tttatccgcg
    23701 aagtgaacgc taactcactg gctcgttacc tgctggtagc cagtgccatt ggttctcttt
    23761 ataagatgaa cgcgtcaatt tctggtgctg aagtcggctg ccagggtgaa gttggtgtgg
    23821 cgtgctcaat ggcggcggct ggtctggcag aactgttagg tgcaagcccg gcgcaggtgt
    23881 gcatcgcggc ggaaatcgcc atggagcaca acctcggtct gacgtgtgac ccggtcgccg
    23941 gacaggtcca ggtgccatgc atcgagcgta acgccattgc ggcagtaaaa gcggtgaacg
    24001 ccgcacgtat ggcgctacgc cgtaccagcg agccgcgcgt ctgcctcgat aaagttatcg
    24061 aaaccatgta cgaaacaggt aaagatatga acgccaagta ccgcgaaacc tctcgcggcg
    24121 gcctggcaat gaagatcgtt gcctgcgatt aatcgctctc cagaggcctc gttttgcgag
    24181 gcctcttccc gatttctcat ccagccgtag cctgttccgg catcgaatgt tacccttatc
    24241 gcctgatctt taagggggtt atcgtggctg ttcatttgct tattgtcgat gcactgaatc
    24301 ttattcgtcg cattcatgcc gttcagggat cgccctgtgt cgaaacctgc cagcatgcgc
    24361 tcgatcagct cattatgcac agccagccaa cccacgcggt cgccgttttt gatgatgaaa
    24421 accgcagtag cggctggcgt catcagcgtt taccagatta caaagcgggt cgaccgccaa
    24481 tgccggaaga gttgcacgac gagatgcctg cattacgcgc cgcctttgag caacgcggcg
    24541 tcccgtgctg gtcaaccagc ggcaacgaag ccgatgactt agccgccacg ctggcggtca
    24601 aagtgacaca ggccgggcat caggcaacga ttgtttcgac agataaaggc tactgtcagt
    24661 tactttcacc gacattacgt attcgtgatt acttccagaa acgttggctg gatgcgccat
    24721 ttatcgataa agaatttggc gttcaaccgc agcagttgcc cgattactgg ggacttgcgg
    24781 ggatcagcag ttcaaaggta ccgggtgttg cgggaatcgg accaaaaagc gccacgcagc
    24841 tgctggtcga gtttcagagt ctggaaggga tatatgagaa tctggatgcg gttgccgaaa
    24901 agtggcgcaa aaaattagaa acccataaag agatggcgtt tctgtgccgc gatattgccc
    24961 gcttacaaac cgatttgcat atcgacggca atttacagca attgcggttg gtacggtaac
    25021 ggcgagccgg atgcaaacga ttatcaacac attgaattta atgcacttat ataccggata
    25081 agacgcggct agcgtcgtat ccggcattat cacatcagcg catttaccag gcggtatggt
    25141 aaagctctac aatatcctca agcgttgctt cacgcgggtt gccaccggta caaacatcat
    25201 ccagtgccgc ctgcgccagt gccggaatgt cttccttgcg tacaccaaca tcacgcaaat
    25261 gtggcggaat accgacatca cggttgagag caaacaccgc ttcaacagcg gcattacgcg
    25321 cctcttccag ccactttcac gcccataacg cgcgcgatat cgcggtactt ctcaccggta
    25381 aagtcagcgt tatagcgcat gacatgcggt aacaggatgg cgttcgcaac accgtgtgga
    25441 gtgttgtaaa acgcgcccag tggatgcgcc ataccatgca ccaaccctaa cccaacattc
    25501 gagaagccca tacccgcaac atactgcccg agcgccattt cttctccggc atccttatca
    25561 ccagcaaccg atcctcgcag cgccccagca atgatttcaa tcgctttaat gtgcagtgca
    25621 tcggttagcg cccacgcgcc acgggtaata tacccctcaa tagcatgagt gagcgcatcg
    25681 acacccgtcg cagctttcag cgctggaggc ataccatcca tcatgtcagc gtcaataaac
    25741 gccacctgcg ggatatcatg cggatcaacg caaacaaact tgcgccgttt ctcttcgtca
    25801 gtgatcacgt agttaatggt cacttctgcc gcagtacctg ctgtggtagg aattgccaga
    25861 atcggtacac tgggtttatt ggtcggggaa agcccttcca ggctacgcac atcggcaaac
    25921 tccgggttgt tgctgataat gccaatcgct ttacaagtat cctgtggaga accaccacca
    25981 atagcgatca ggtaatccgc gccgctattc tggaatacac cgagcccttc tttgacgaca
    26041 gtaattgttg ggttgggcac tacgccgtcg taaatcgccc atgccagccc tgcagcatcc
    26101 atcttatcgg tcactttcgc caccacgccg cattgcacca gcgttttatc ggtgacgatc
    26161 agcgccttct gataaccacg gcgtttcacc tcatcggtta aagccccaac agcaccccga
    26221 ccaaaccatg ccgtttcgtt cagaatcatt ctgttagcca tcatccttct ccttgttgct
    26281 ttacgaaatt actcttcaat ccgtaaccca taggttttga atttctccag cactacggca
    26341 atctcttcat cgctcagcac tggcaccggg tccgtaatcg ccagggtcgt caggtaaagt
    26401 tgcgccagca cttcaacttc atgcgccagc cataacgctt tttccagatt cacctcacaa
    26461 gcgataagcc catgatgttg taacaaagtt gccttacgat ttttgagagc cagcgcaaca
    26521 tgttcagaaa gttcgcgtgt tccaaaggtc gcataaggcg cgcaaggaat agaattacca
    26581 cctgccgccg caatcatgta gtgaatagcg gggatcggtc ggttaagaat ggaaactgct
    26641 gtgcaatgaa cggcatgatt gtgaacaacc gcgttggcat ccggtctgct ttgataggcc
    26701 gccatatgga aacgccattc gcttgagggg agctttcctt cctcatgttt accgttacca
    26761 tcaataaagg caatatgcga ctccgtcagt ttttcatatg gaatgcctgt aggcgtaatc
    26821 agcatcccat cctgataacg tacactgacg ttccccgctg tcccctggtt cagtcccagg
    26881 cgggtcattt ccaggcaagt gtcaataatc tgacgagcaa gtttatttcg ttccattagc
    26941 tacctctctc tgattcaaaa cagggcaata atgttgttcc tttcacacta ttgaattagc
    27001 cgtttaatta cccaccat
//
LOCUS       DAJVTY010000041        26605 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVTY010000041 DAJVTY010000000
VERSION     DAJVTY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26605
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.041"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..151)
                     /locus_tag="OQQ66_001553"
     CDS             complement(<1..151)
                     /locus_tag="OQQ66_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1904525.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPN"
     gene            complement(348..1121)
                     /gene="hisP"
                     /locus_tag="OQQ66_001554"
     CDS             complement(348..1121)
                     /gene="hisP"
                     /locus_tag="OQQ66_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1904526.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1129..1845)
                     /locus_tag="OQQ66_001555"
     CDS             complement(1129..1845)
                     /locus_tag="OQQ66_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1904527.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(1842..2528)
                     /gene="hisQ"
                     /locus_tag="OQQ66_001556"
     CDS             complement(1842..2528)
                     /gene="hisQ"
                     /locus_tag="OQQ66_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1904528.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2618..3400)
                     /gene="hisJ"
                     /locus_tag="OQQ66_001557"
     CDS             complement(2618..3400)
                     /gene="hisJ"
                     /locus_tag="OQQ66_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1904529.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3621..4403)
                     /gene="argT"
                     /locus_tag="OQQ66_001558"
     CDS             complement(3621..4403)
                     /gene="argT"
                     /locus_tag="OQQ66_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1904530.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4669..5238)
                     /gene="ubiX"
                     /locus_tag="OQQ66_001559"
     CDS             complement(4669..5238)
                     /gene="ubiX"
                     /locus_tag="OQQ66_001559"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1904531.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(5333..6850)
                     /gene="purF"
                     /locus_tag="OQQ66_001560"
     CDS             complement(5333..6850)
                     /gene="purF"
                     /locus_tag="OQQ66_001560"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1904532.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(6887..7375)
                     /gene="cvpA"
                     /locus_tag="OQQ66_001561"
     CDS             complement(6887..7375)
                     /gene="cvpA"
                     /locus_tag="OQQ66_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1904533.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7634..8296)
                     /gene="dedD"
                     /locus_tag="OQQ66_001562"
     CDS             complement(7634..8296)
                     /gene="dedD"
                     /locus_tag="OQQ66_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1904534.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8286..9554)
                     /gene="folC"
                     /locus_tag="OQQ66_001563"
     CDS             complement(8286..9554)
                     /gene="folC"
                     /locus_tag="OQQ66_001563"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1904535.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9624..10538)
                     /gene="accD"
                     /locus_tag="OQQ66_001564"
     CDS             complement(9624..10538)
                     /gene="accD"
                     /locus_tag="OQQ66_001564"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1904536.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10694..11353)
                     /locus_tag="OQQ66_001565"
     CDS             complement(10694..11353)
                     /locus_tag="OQQ66_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1904537.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11436..12248)
                     /gene="truA"
                     /locus_tag="OQQ66_001566"
     CDS             complement(11436..12248)
                     /gene="truA"
                     /locus_tag="OQQ66_001566"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1904538.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAPRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12248..13261)
                     /locus_tag="OQQ66_001567"
     CDS             complement(12248..13261)
                     /locus_tag="OQQ66_001567"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1904539.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13327..14463)
                     /gene="pdxB"
                     /locus_tag="OQQ66_001568"
     CDS             complement(13327..14463)
                     /gene="pdxB"
                     /locus_tag="OQQ66_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1904540.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14562..15557
                     /gene="flk"
                     /locus_tag="OQQ66_001569"
     CDS             14562..15557
                     /gene="flk"
                     /locus_tag="OQQ66_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1904541.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15554..16732)
                     /locus_tag="OQQ66_001570"
     CDS             complement(15554..16732)
                     /locus_tag="OQQ66_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1904542.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17061..18281)
                     /gene="fabB"
                     /locus_tag="OQQ66_001571"
     CDS             complement(17061..18281)
                     /gene="fabB"
                     /locus_tag="OQQ66_001571"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1904543.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18440..20446
                     /gene="mnmC"
                     /locus_tag="OQQ66_001572"
     CDS             18440..20446
                     /gene="mnmC"
                     /locus_tag="OQQ66_001572"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1904544.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20567..20845)
                     /locus_tag="OQQ66_001573"
     CDS             complement(20567..20845)
                     /locus_tag="OQQ66_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1904545.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(20879..21427)
                     /gene="epmC"
                     /locus_tag="OQQ66_001574"
     CDS             complement(20879..21427)
                     /gene="epmC"
                     /locus_tag="OQQ66_001574"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1904546.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21427..22236)
                     /locus_tag="OQQ66_001575"
     CDS             complement(21427..22236)
                     /locus_tag="OQQ66_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1904547.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22236..23060)
                     /gene="mepA"
                     /locus_tag="OQQ66_001576"
     CDS             complement(22236..23060)
                     /gene="mepA"
                     /locus_tag="OQQ66_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1904548.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23064..24149)
                     /gene="aroC"
                     /locus_tag="OQQ66_001577"
     CDS             complement(23064..24149)
                     /gene="aroC"
                     /locus_tag="OQQ66_001577"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1904549.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24184..25116)
                     /gene="prmB"
                     /locus_tag="OQQ66_001578"
     CDS             complement(24184..25116)
                     /gene="prmB"
                     /locus_tag="OQQ66_001578"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1904550.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25282..25833
                     /gene="smrB"
                     /locus_tag="OQQ66_001579"
     CDS             25282..25833
                     /gene="smrB"
                     /locus_tag="OQQ66_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1904551.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(25871..26584)
                     /locus_tag="OQQ66_001580"
     CDS             complement(25871..26584)
                     /locus_tag="OQQ66_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1904552.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6549 a         7282 c         6718 g         6056 t
ORIGIN      
        1 tgtttggttc cagtttaagc gtcgttaaat cacacagttt gcgcagcggc gcgggaagat
       61 gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta aataccgcat
      121 catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca aatttaagat
      181 gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc tttggacagc
      241 gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag ctgactcgca
      301 aattccccat gaaaatactc cggcagcgtt agccaccgga gaggaaatta tttcagcgat
      361 cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg ctccggcgcg
      421 ccctcttctt ctattttccc ctgatggagg aaaatgacat gagtagaaac atggcgagca
      481 aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag ttgctgcata
      541 atacgcaaca cttcgcctac cagttcagga tcgagcgccg aggtaggttc atcaaacagt
      601 aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg ctgaccgccg
      661 gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt tgccagatac
      721 ttgaccgccc gctcgtgcgc ttcctgcttg ctcaggccca acacctgaat cggcgcttcc
      781 atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa taccatcgtc
      841 aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg accgtctttg
      901 tcgcgcacca gattgatcgt ctggccattg accacgatcg acccttcact cggtttttcg
      961 aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc gatgatgctt
     1021 attacatctc cggcattcgc ttgcagtgag acccctttca gcacttcatg ttcgccgtag
     1081 cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc agtgcgttga
     1141 agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc tgatcaggac
     1201 ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag gttgatacgt
     1261 ggcggcgtta atatcgcggg ctattttcag cagatccggc acagtggccg taaacgccag
     1321 cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg caatacgcag
     1381 cgcagaaggc aaaataatgc aacgatacat tttaaaagtc gagaagccat aggctctggc
     1441 ggcttcaatt tccccatgca gaaccgaacg gattgcccca gcaaaaatct cggtagtgta
     1501 agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc ggaagaaagc
     1561 gttaaggaat tcggttccct taacaatctc aagcgtgtat atgccggaat agaacaccag
     1621 caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga ttggaaactg
     1681 gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga ctccgcctat
     1741 cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc gataaccgtc
     1801 ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc agccctcttc
     1861 acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac agtggtgaaa
     1921 accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt acttttgcct
     1981 gccagttggg tggctttgac cacatcttcc aggccgagta acgaaaccag tgcggtagat
     2041 ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat catcgccgga
     2101 aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc cgcctctata
     2161 tgtcctttcg gcactgccat aaaagcacca cggaacgttt cggtaaaata agcaccgtag
     2221 ataaaaccga gagtgataat accagcgacc atcggatcga tatcaatctg cccgacgccc
     2281 atcgcctccg tcaccgtgtt tagcgcaatc tgcaaaccga agaaaatcag cagcatcaac
     2341 actaagtccg gcacgccacg aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc
     2401 cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc gagcactaca
     2461 gagctgatag ccagctccag cgtgacgagc gccccctgta aaataacacc tgaaaaccca
     2521 tataacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt
     2581 tacccgaagg gagcgggctt tctttcaccg atggcgatta gccaccataa acatcaaaat
     2641 cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt
     2701 tgttcagtgc ttcgcgcagt tcgttatctt ctttacgcag gcccatgccg gtccctacgc
     2761 caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta ccgacaggtt
     2821 gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca atacgtccgg
     2881 cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca atgccttttg
     2941 gtgcccaatg ttcattaccg aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt
     3001 tgcctttcag cgactcgact gtcggctgaa tgtcagaatt tttcgccacc accaaacgag
     3061 aatcggcagc gtacagtttg tcggtgaagg ctatttcttg ctgacgtttt tccgtaatgg
     3121 aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt aacgcatcca
     3181 gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc tttgccagat
     3241 cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc gcataggtcg
     3301 ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa tgccgcagtt gcgctggaga
     3361 aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca actgacagtc
     3421 tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat tgaccatcaa
     3481 ggcaaaatat cccgccccgt tagatgtatt atttttcatt aaaaatgctt aagtatgcat
     3541 ttcaatgcac cacgatagtg caacaaacct agcgtgcacc atgatgatca ttccatcagg
     3601 tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata ctttttcgcc
     3661 atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa ggcagccgtc
     3721 agttcagcat catctttacg tagccctaca ccggtgccat caccgaagta ttttttgtct
     3781 tttactgatg agccagcaaa ggcgaaatct ttaccagcag gttgcttgag gaatccttcg
     3841 ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag atcggaatag
     3901 accaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca ggtctcgtta
     3961 gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt cagtgaatcc
     4021 agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc ggcgtacagc
     4081 ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga cgaaataatg
     4141 gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact ggcaacccag
     4201 gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc aaagccaaca
     4261 aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt tccgatacgt
     4321 accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac taacaaagac
     4381 agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt tttatgttgt
     4441 gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct tgacgacaac
     4501 agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtcctg aagttgaaga
     4561 tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag
     4621 gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt atgcgccctg
     4681 ccagcgggca aagagatctt caggaagggt tattgcaaac tggtcaagaa cacgattaac
     4741 cgtctgattt atcacatcat caagggattg cgggcgatga taaaacgccg gaacaggagg
     4801 cataatcacc gcaccgattt ctgccgcctg agtcattaaa cgcagatggc ctaagtgcaa
     4861 tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca catctgccgc
     4921 acgggtcagt aagccatcag tatagctatg gacaatgccg gaaagggttt tgattgaaca
     4981 gggtaaaatc accatcccca gcgtctggaa agaaccggaa gagatgctgg cggaaatatc
     5041 gcgcgcatcg tgcgtgacat cagctaatgc ctgcacttcg cgcagggaaa aatccgtttc
     5101 gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga tatctgtgac
     5161 atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac cgctgatgcc
     5221 tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac tttgcagtat
     5281 ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg catcatcctt
     5341 cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg gcgtcatcat
     5401 tacgtaacgt atcgaggaaa tcgaggtagt cctgatcaac atctttggtg acgtagacgc
     5461 cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga acggcgtcga
     5521 tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat
     5581 caacttcgcg accgtgagcg atcagttccg tggcgctcgg catatcaata ccataaacgt
     5641 tcgggaagcg aatttccggg gccgcagaag cgaggtacac tttcttcgct ccggcttcgc
     5701 gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg tcgaccagca
     5761 ggacgttttt atcgcggaac tcggcgcggt tggcgttcag tttacggcgc acggacttac
     5821 gacgcagctg ctggcccggc atgatgaagg tgcggccaac atagcggttt ttaacgaagc
     5881 cctggcggta cggcttgccc agaatacgag caatttccag cgcgatatca cacgaggttt
     5941 ccgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca attttctcac
     6001 ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg ataaacgagt
     6061 ccgggcgggc aaagtataca tactcaaaca ggcacggatt gctgaccgga ttgtcagcac
     6121 attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc ggcgcgacgt
     6181 cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg accatatatt
     6241 ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc ccgtttggat
     6301 cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatat gcgccgcgga
     6361 ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc gggtagtggc
     6421 ggaagttgtc cagctcgcta gcgaagatat taagcagaat ttccgagtcg gaagtggtgt
     6481 tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat
     6541 tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc tgcgcttcag
     6601 aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata ttgccctgca
     6661 aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct ttacgcaaac
     6721 ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg cgatgctgaa
     6781 gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg ataccgacaa
     6841 taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac gctctgggca
     6901 agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa ctgaactgcg
     6961 ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa
     7021 agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag acgcccagca
     7081 cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg aagttcacga
     7141 tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaattcca tttcgaacca
     7201 gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga ctggcaacaa
     7261 agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg cggatcaggc
     7321 taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag accatatgtg
     7381 tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg
     7441 tttgcgtagg gatttccttc ccacgcatca ataaaaatgg cgctgaaaaa atattcaacg
     7501 ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg gcgtaaacgc
     7561 cttatccgac ctacggttct aacactgccc aagcctgata agacgcgcca gcgtcgcatc
     7621 aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag ccagaaagtt
     7681 gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc ccaaccagaa
     7741 tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga taaccggcac
     7801 cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca cccagttgca
     7861 caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc ggttctggct
     7921 tcggcgcagg tggtgcttca accttcggtt taggcggctc caccggtttc ggcttcggtg
     7981 gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata gtggccggat
     8041 cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct tccggcggct
     8101 gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga tcgcccgctt
     8161 ttggaaccag cgggatagcc gcaaactcat cctgataatg ttttttctgc ccgtccagca
     8221 gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact aaccgattct
     8281 gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg tgcgaccgtg
     8341 tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat tgcggcatcc
     8401 catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag cagttgttct
     8461 gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac cacgcttttc
     8521 aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac cgccagcacg
     8581 cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc atgtggatta
     8641 tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg tcccggcaaa
     8701 attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc gctggcacgc
     8761 agtgccgcca gcgctgttgc ggcattcggt tgcgggacaa gcggcaacgg cagattttcc
     8821 agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata gttccactca
     8881 acgccccgac gttgtaacag tgcacctttt tcctgcgcca catcagcaat ggtagaaggc
     8941 atttccggct caccgacaat tgccggtttt gcgctgcgaa agatgcctgc tttctcgcga
     9001 ccaatacttt cgcgatctgg ccccagccag tcggtatgat ccagcgcaat actggttact
     9061 accgcgacat cggcatcgac aatattggtt gcgtccagac gaccgcccag ccctacttcc
     9121 agaatcacca cgtcaagttg tgcctgtttg aacagccaca acgccgacag cgtaccgtac
     9181 tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa agaggcggtg
     9241 tgggccgatt ccggcaattc ctgcccctgc acacgtacgc gctcggtata acgcaccaga
     9301 tgcggcgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga ctccagcgta
     9361 cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg cgctggtttc
     9421 agaacgccaa gacgagccgc gacctggctc acgcgctcaa ggccgagatc gatagtttta
     9481 ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc ttgaggagtg
     9541 cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa gggcagagcc
     9601 agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac cgggggtacc
     9661 actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa cttcgccaga
     9721 atgctcgcca gtttcaggcg catttccgga cgacggacga tcatgtcgat cgcgcctttc
     9781 tcgatcagga attcactgcg ctggaatcct agcggcagtt tttcgcgaac ggtctgttcg
     9841 ataacacgcg gaccggcaaa gccaattaac gctttcggtt cagcgatgtt gagatcgccc
     9901 agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac ggagatgtac
     9961 ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat ctgcatcagc
    10021 gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat cagcgggcag
    10081 ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg cacccacaac agaccccatt
    10141 gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc atacagagtg
    10201 cctttcatca ccaccagcgc atctttttcg ccggtttctt tctgcgcaga agccagacgg
    10261 tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag ctcgctaccc
    10321 atctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc tgtcatacgc
    10381 atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc gcggtataaa
    10441 acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc cttgcgggtg
    10501 ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc tgtctgaacc
    10561 tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc ccaacagacc
    10621 atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag tggtcgaacc
    10681 gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacga ataaattcga
    10741 aaaccccagg caatattgaa acaaaaataa tgccaacaat catcagctta aggttgctct
    10801 gaatgaaggg gagcatgccg aagaaataac ccgcgtaggt aaaaatcagt acccacaaca
    10861 gtgcgccgat cacgttataa gcggcgaaat gacggtacga catgtggccc attcccgcaa
    10921 caaacggcgc gaacgttctg acgatcggta caaaacgggc gagaataatc gttttgccgc
    10981 catgtctctc gtaaaactga tgagtcttat caagataact gcaacgaaag attttcgaat
    11041 ccgggttact gaacagtttg tcaccaaata accgtccaat ggtatagttc actgcatcac
    11101 cgacaatagc ggcaatcaac ataagcacca ccatcatatg gacattgaga tcgttagttt
    11161 ccagcgacgc caatgcacca gcaacaaaca gcagcgaatc accaggcagg aatggcgtta
    11221 ccaccagccc cgtttcgcag aacagaatca aaaacagaat ggcataaacc cagacgccgt
    11281 actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg atgaggaaat
    11341 aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg taactacgtt
    11401 ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa tagcgggccc
    11461 attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag atacagccct
    11521 tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa ctctgctatc
    11581 cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat attcctgacc
    11641 atatgatgta caaaggcatt cgctttgata tctaccacca cataaggacc gtgacgcgtg
    11701 acattaatat gcataacgtt gcgccacggg gttcgggact ggcactgcac cgcacggaac
    11761 gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg ttcagcgtcc
    11821 agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag ccgatgattg
    11881 tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa atcatcaggt
    11941 acagctttaa cccaacgcac ggcgatgtca tcaggtaaat tcgcatttac gcccaaggtc
    12001 cacgccgcgt ctttgcgcgg cgcggttgtt tcgaaatgca ccacctgccc ggtaccgtgt
    12061 acccctgcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc cacctgggag
    12121 agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg ccagccgtaa
    12181 tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcag ttgttgctgg
    12241 tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact gccatcagcg
    12301 cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc ggcataccgt
    12361 agtcattacg cacgcagcca acagaaagat gcggagtacc cgaagcatcc cctacctgag
    12421 tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac gcatcacgcg
    12481 cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca tgaccgtaga
    12541 ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc aggattttgc
    12601 gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc ggcaacaacg
    12661 gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc ggaatgccgt
    12721 tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc tgggcggagg
    12781 ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt aacggtttca
    12841 gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc cggttgcggt
    12901 aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg ggttcgagag
    12961 caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct tcaacccagg
    13021 cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc cagtcgaatt
    13081 cagcggcatc ctgcacggtg attgttttac caccaaagcg cagttgttcg cctgcgcttt
    13141 cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc agcgtttcaa
    13201 gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag ccttcagaca
    13261 tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga gagcatgaag
    13321 aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca gcaatgatgc
    13381 cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct caagatagtt
    13441 tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg gtgcgtcatc
    13501 gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat cgagcgggcc
    13561 atgcagcgta atgcgaccaa actctggcgc gggcagtaat gtatccagcg caacgtgctg
    13621 ttcatgccca ataaacttgc tataagcttc aaacacttgc gtagtaccgc gtgctttacc
    13681 ttccagggta taacctgcga tatgcggcgt gccgatatcc acttttgtca gcaactccac
    13741 gttaagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct ggccttcatt
    13801 caggcaggtc agcaacgcag tattatcgac gactgcgcca cggcaggcgt taatcagaat
    13861 cgctcccggc ttcaggctac ggatcagttt ttcatccgcc agatgcagcg ttttgtacgg
    13921 cccatctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa ctaattcatc
    13981 cagcgagcgg aaatcaccct catccccacg gtcagcgcga ggcggatcgc aaagcaatgt
    14041 tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac caacgcccac
    14101 gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca tcagtagtga
    14161 ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg aaaaaccaat
    14221 tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc cccgcagtgg cagtgccaac
    14281 aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg aacgcaccat
    14341 cagcgcatcc gcgtctgcca gttgagcgac ggcgattgga cgcccgggaa ccgcggtcac
    14401 ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa caaggatttt
    14461 cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtgtgcca taatctcgcg
    14521 gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa cctatttccg
    14581 gccctcctcc tgggcaacca ccaggtcagg gagataacct gccgtctggc gcgggcaatc
    14641 agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa gtgacctcac
    14701 tgacgcaaca gcaaagagca gaactgtggg cgggtatcag gcacgatatt ggtctgtcgg
    14761 gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcataatctg gcgcaacgtc
    14821 tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta ggggagtatt
    14881 tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac tttggtcaga
    14941 cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg ttgcaggaag
    15001 ggaagatggt tattccgcaa ccgcagcagc gcgaggcgac cgaccgtcct ttattaccgg
    15061 cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc ggcggcaacg ggggaaccca
    15121 gcaaacagat ctggcaatcg atgctggaac tttccggggt gaaagatggc gagttaattc
    15181 cagcgaaact gtttaaccat ctggtgacct ggctacaggc gcgtcagacg ctaagccagc
    15241 aaaatacgcc gacgctggaa tcactacaga tggcgctaaa acaaccttta gatgccagtg
    15301 aactggcggc gttatcggca tatatccagc aaaaatatgg cctttctgcg caatcatcgc
    15361 tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg gttaaaggga
    15421 ttgatccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg gacacgttgc
    15481 aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc ctgatgctgg
    15541 tctggctggt tcgttaaccc cgacgaaatg acagtatcgt gacaataata cccagcaccg
    15601 cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc agcattcccg
    15661 ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg cccagtgcgg
    15721 tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca ggaaagataa
    15781 gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccatgca cctggggctt
    15841 gccagagcag caacaagccc accgtttcta caagcagaga gacaatcgcc actttcacgc
    15901 cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat gcgccgccaa
    15961 acgcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag agcgaaacga
    16021 aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag ccctaaccct ggtttccaga
    16081 taagcccgac aacgctccac agcgatggac gttctcccgc cagggccggt actttgcgca
    16141 ctgtgccgtt acaggcccac gccagtaagg gtaataccat tgtggtgatc gccagtgcgg
    16201 caaaaccgta atggctatga atcaacaggc caagcggcgc accaacagcg agggcaccgt
    16261 aaatcgccat tccgttccat gacatcactt tgccagagtg ttttgcccct acgatgccta
    16321 atccccaggt cagagcgcct gtcagtaact ggctttcacc aaagccaaga atcaaacgcc
    16381 cgacgaccaa cagggcaaat ttcaacggtg cggagacagg caaaatcgcc gccagcagca
    16441 acgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt tttgcaccat
    16501 attgatcggc cagtcgcccg gcatagccac gcgtcagcac cgtagccaga aactgaatcc
    16561 cgacggcaat cccgaccatg gtattgccat agcccagatc atgatgaaca aacagcggga
    16621 taaccggcaa cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa gcgatgcgga
    16681 agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc atgcattact
    16741 ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt cagggttaca
    16801 gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa agcgggaagt
    16861 ttgcctggaa ctggcggcga ttgtcaatga tgtgaaaaag ggaaccatca ggttcccttt
    16921 tgcgttagtg ccggaggccg cattggcgcg taacgtcgga tgcgacgctg gcgcgtctta
    16981 tccgacctac tccgactact ccgactactc cgactactcc gactactccg actactccga
    17041 ctactccgac ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg
    17101 ccgccgaagc cgaagctgtt agacataacg gtggtcagtt cgcgatcggt cgtttcggtc
    17161 acgatgttca gacctgccgc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata
    17221 aagccgtgtt ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga
    17281 gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca
    17341 cggatagctg ccagttcttt cacgtcgcca accggagtcg aagtaccatg agagttcagg
    17401 taatcgattg gggtatcaac gccgtgcatc gccatcttca tgcagcgtac tgcgccttcg
    17461 ccagacggag caaccatgtc tgcaccatca gaggttgcgc cgtagccaac gatttcagca
    17521 tagatgtgag caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgccg
    17581 ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg
    17641 gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga attcgcaggc catttcccag
    17701 cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct
    17761 actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg
    17821 ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg
    17881 accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaat
    17941 acctggaaac gcggggaacc gccgccagaa cctgcaatca ggccaacgcg cgggttattc
    18001 tggtaagctt ccggagaaag gcccgcatct gcgattgcct gctccataga aaggaatgca
    18061 taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc
    18121 agttttacgt tgccccagac gtggctacgc atgccggaat ccttaagctc ctgagagaaa
    18181 gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg
    18241 ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg
    18301 cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc
    18361 agccatttaa tagagaaatt tgcgcagcct tacacacatc gctaagatcg agccaccgcc
    18421 tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa
    18481 tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg
    18541 gctggaagag acgcgttatg tttttctggg aggcaaccaa ttagaggtac gctttcctga
    18601 gcatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct
    18661 gacgctatgg caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg
    18721 cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca
    18781 tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc
    18841 cttgcccggt tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt
    18901 tggcgatatt aacgaactga ccagccaaca ggacgattcg ctaaatcaaa aagtagatgc
    18961 ctggtttctg gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt
    19021 taacgccatg gcaaggttgg cgcgtccggg cggcacgctg gcgacattta cgtctgccgg
    19081 ttttgtccgc cgcggtttgc aggacgccgg attcacgatg caaaaacgta agggctttgg
    19141 gcgcaaacgg gaaatgcttt gcggggtgat ggaacagaca ttaccgctcc cctgctccgc
    19201 gccgtggttt aaccgcacgg gcagcagcaa acgggaagcg gcgattatcg gcggtggtat
    19261 tgcctgcgcg ttgttgtcgc tggcgctatt acggcgcggc tggcaggtaa cgctttattg
    19321 cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt
    19381 attaagcaaa cacgatgagg cgctaaaccg ctttttctct aatgcgttta cttttgctcg
    19441 tcggttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt
    19501 aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc
    19561 agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa
    19621 ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa
    19681 tgtgctggaa ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagaa
    19741 tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca
    19801 cagcgtagtg gtactggcga acgggcatca aatcagccga ttcagccaaa cgtcgactct
    19861 cccggtgtat tcggttgccg ggcaggtcag ccatattccg acaacgccgg aattggcaga
    19921 gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcaaca
    19981 tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga
    20041 tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga
    20101 ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct
    20161 gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgttggc
    20221 ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac gatctcttta tgtttgcggc
    20281 tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat
    20341 gagcgacgaa ccgattccga tggatgccag tacgctggcg gcgttaaacc cgaatcggtt
    20401 atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt
    20461 gtttgccgga tgcggcgtga acgccttatc cgacctacgt gtgacgtgta ggcctgataa
    20521 gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac tgctgtgacg
    20581 cctgctgaaa caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt
    20641 caccggcacc aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg
    20701 agtggtcatc accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc
    20761 cactggcaaa caactcatca tcactggcat ggtcaatcat gccgtcgatt aatgccagaa
    20821 tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg caccggcttc
    20881 agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat
    20941 tttgtagtgc tttaatgaag cgtgccggac gctcgggaat gccatttgtc agataatcca
    21001 tcacctgcgc atgtacccgc cgttgaaaca ccacacgatc cggttcgaag tcgccttcca
    21061 gattgtcgca actgacgtta aacggatatc ccgccgccac acagaacaac cagtcgagtg
    21121 cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ttgggcatca cgcccatccg
    21181 ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc ttttccggca atacaccagt
    21241 gcgaaatctc atgaattgcg ctggcataaa agccgtgggc aaagacaatt cggttatacg
    21301 gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat
    21361 taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac
    21421 tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat cgtaaagtaa
    21481 tttggcactc atcaccgccg agacgataac gatcatcggg cggatcagtt tttgtccttt
    21541 gctcagcacc agtcttgaac ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa
    21601 gcccgtcgcc caaatcactt tgccgccgag aataaacagc agcagaccgc caatgtttga
    21661 cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg aggttgaatc cgcacagcgt
    21721 aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aagaatccgt cataaaaacc
    21781 gacgcagcca ccggcaatta acgcgaaggg taaaccgtac atccggcgct ggcgatcttc
    21841 ttcacccagc tttggcatca gcaagaaata gaggccgata caaattacca gaatgggcaa
    21901 aatctgccgt aagacatcag cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc
    21961 ggcaaaggtc atggcgatat tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat
    22021 aaagtagata gtggcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc
    22081 gttagcggga gacattcccg cagccatcaa tgccggaatg gtgagtaacc caccgccacc
    22141 ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag
    22201 cggggaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc
    22261 gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcctggt
    22321 tttggaggtg caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa
    22381 ggttgatctt cacactccag actatcggca gggcaacgta atcgtacatg catatgcgcg
    22441 cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg
    22501 caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg
    22561 gcgagtttga tcaagctgaa aatttctggc ttccacagcg tggagacaac gtgtttaccg
    22621 tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt
    22681 ttcggcagtt gcagaaagat atcgacatcc agtccggtct ggtggctggc atgaccgccg
    22741 ttgaaacgcc caccagcggg catccccata tcgccaatca gcaccgtacc catgcccaga
    22801 ttgctcacct ggctactcag acgctggata aacatcacca gatccgggtg accgaaatag
    22861 cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa cgtgtcagcg
    22921 ccgacaatac agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg
    22981 gttatttttt gccacggcgt cgctgccaag ctagcgctac tggcaagcag agccagcagc
    23041 gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt
    23101 tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc
    23161 ggcactgcac ggatcccgac acagggatcg tgacgacctt tggtgatcat ctcaacttct
    23221 tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc
    23281 atatgagcaa tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg
    23341 ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccgcgcag cgccactacg
    23401 tcaaagccgt cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg
    23461 atgtcggcat ccaggcggtc aaataccggc tcgccaagtc cggcggggac gccactggca
    23521 acaacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca
    23581 tctaacgcgt cgattttgtc cggatccggg cagaagaacg gattttgttc aaccagcgac
    23641 cagtctttga tttccagcgg aatgtcgccc atctgggtca agcagccgcg gatttcaata
    23701 ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt
    23761 tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag
    23821 gtgtaatcag catggcctgg gcggaaaacg tctttaattg cgctgtaatc ctgagagcgc
    23881 tggtcagtgt tttcgatcaa caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca
    23941 ccagagagaa ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca
    24001 gggcgacgac ggtcgaggtc atgttgcagg tccgcttccg tcagcagaat gcctggcgga
    24061 acaccatcga cgatgcagcc gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga
    24121 aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt
    24181 tacttaatct ttataaatcg cgaaatattc acgggccgcc agcagctgct ctttggtgag
    24241 cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata
    24301 ttgttccata agatgtacca tgctgttgcc gacttcacaa atcaacacgc catcatcagc
    24361 aaggtaatct gccgcgtttc caagaatgcg acgcgtcagt ttaaggccgt cggtgccaga
    24421 tgccaggccc agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc
    24481 gtcgacatac ggcgggttag tgacaatcag atcgtactgc actttcggca agtcgcggaa
    24541 cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc
    24601 agcaaccgcc agcgcgtcgg gagagatatc caccgcgtcg acttctgcat ccgggaaggc
    24661 ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg
    24721 ttgtttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg
    24781 caccagcacg cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag
    24841 gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa tacgatgttt
    24901 ttcgctggag gtcagacgcg cggtgcgcat atcttccggg atatccagcg gcaggtagag
    24961 cgatggtaac accagctgta cggcttcatc ccacgggtta tcggtgccgt gaccgtacca
    25021 gatatttgcc gcgctgaagc ggctcactga ccagcgcaac atgtcctgaa tggtttgcag
    25081 ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca
    25141 ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga
    25201 gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa
    25261 attagaaata gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct
    25321 ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga
    25381 ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat
    25441 gccagccatt atttctccga tgagtttcag ccgttattaa ataccgaagg tccggtgaaa
    25501 tatgttcgcc cggatgtcag ccatttcgag gcgaagaaac tgcgccgcgg cgattattca
    25561 ccggaattgt ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc
    25621 gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac
    25681 gggaagcata ttttgaagca acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg
    25741 gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt gttgattgaa
    25801 gtggaagagt ggttgccgcc ggagttgccc tgaggagttg agcgtgttgt gccaggtgag
    25861 aaaaggtgcg ctaatacata ttcagtatgt aagtgaatcg gcaggtagaa tctatgggct
    25921 gtcgagatgt cgtgtccacg cgtagcttca tatcatgcag ttgcctggac gtaatcggtt
    25981 gcggctcagg cgtcaccgtc actttggcat tatcacagcc ggaagtcgag aaggtcatct
    26041 gctgtgcgat aaaacggcag tagacaccac gctcatcaaa attattcccg ttacacagtt
    26101 tgccattttc catcaggaaa gtgggcagtg tttgctcaat aacaccacct gttgactgca
    26161 acctgacgac tttgttatgt acaacataat tttcattcca ggaaacatca tacggtgcca
    26221 ggggcaccgc cggacatgaa gaactcacct tcgttgggcc aatatagtca ggaaaatcat
    26281 catcatacgt tttagcgaag ggatctctac tcttaacaaa cgacgacaca atccacggac
    26341 aaccatgtaa attcccatcg taataaacaa acatctttaa ttcattacat gtccatcccg
    26401 ttttggtctg gctatcatac tgctctaatc caggacaggc tgacatggat accctgctgt
    26461 ccgaaatttt cgacatagat ggttgagtag aactatcgcc agtccacgaa actaaatccg
    26521 gctgtgaatt gggcatgaat gtagccttaa atatagcgta attaactgaa tctggcgtcg
    26581 ctataatcag atgaaactca aactc
//
LOCUS       DAJVTY010000042        25353 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVTY010000042 DAJVTY010000000
VERSION     DAJVTY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25353
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.042"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1849)
                     /locus_tag="OQQ66_001581"
     CDS             complement(<1..1849)
                     /locus_tag="OQQ66_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1904553.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKK"
     gene            complement(1960..3372)
                     /gene="ftsP"
                     /locus_tag="OQQ66_001582"
     CDS             complement(1960..3372)
                     /gene="ftsP"
                     /locus_tag="OQQ66_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1904554.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3447..4184)
                     /gene="plsC"
                     /locus_tag="OQQ66_001583"
                     /gene_synonym="parF"
     CDS             complement(3447..4184)
                     /gene="plsC"
                     /locus_tag="OQQ66_001583"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1904555.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4417..6675)
                     /gene="parC"
                     /locus_tag="OQQ66_001584"
     CDS             complement(4417..6675)
                     /gene="parC"
                     /locus_tag="OQQ66_001584"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1904556.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7221..7703)
                     /locus_tag="OQQ66_001585"
     CDS             complement(7221..7703)
                     /locus_tag="OQQ66_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1904557.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(7756..8148)
                     /gene="ygiW"
                     /locus_tag="OQQ66_001586"
     CDS             complement(7756..8148)
                     /gene="ygiW"
                     /locus_tag="OQQ66_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1904558.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSRRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8300..8959
                     /gene="qseB"
                     /locus_tag="OQQ66_001587"
     CDS             8300..8959
                     /gene="qseB"
                     /locus_tag="OQQ66_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1904559.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            8956..10305
                     /gene="qseC"
                     /locus_tag="OQQ66_001588"
     CDS             8956..10305
                     /gene="qseC"
                     /locus_tag="OQQ66_001588"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1904560.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDS
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10415..10996
                     /gene="mdaB"
                     /locus_tag="OQQ66_001589"
     CDS             10415..10996
                     /gene="mdaB"
                     /locus_tag="OQQ66_001589"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1904561.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11027..11341
                     /locus_tag="OQQ66_001590"
     CDS             11027..11341
                     /locus_tag="OQQ66_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1904562.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11389..13281)
                     /gene="parE"
                     /locus_tag="OQQ66_001591"
     CDS             complement(11389..13281)
                     /gene="parE"
                     /locus_tag="OQQ66_001591"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1904563.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13310..13891)
                     /gene="yqiA"
                     /locus_tag="OQQ66_001592"
     CDS             complement(13310..13891)
                     /gene="yqiA"
                     /locus_tag="OQQ66_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1904564.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(13891..14718)
                     /gene="cpdA"
                     /locus_tag="OQQ66_001593"
     CDS             complement(13891..14718)
                     /gene="cpdA"
                     /locus_tag="OQQ66_001593"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1904565.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(14743..15165)
                     /locus_tag="OQQ66_001594"
     CDS             complement(14743..15165)
                     /locus_tag="OQQ66_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1904566.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTTVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15166..15795)
                     /gene="nudF"
                     /locus_tag="OQQ66_001595"
     CDS             complement(15166..15795)
                     /gene="nudF"
                     /locus_tag="OQQ66_001595"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1904567.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16000..17481
                     /gene="tolC"
                     /locus_tag="OQQ66_001596"
     CDS             16000..17481
                     /gene="tolC"
                     /locus_tag="OQQ66_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1904568.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            17629..18300
                     /locus_tag="OQQ66_001597"
     CDS             17629..18300
                     /locus_tag="OQQ66_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1904569.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18306..19466
                     /locus_tag="OQQ66_001598"
     CDS             18306..19466
                     /locus_tag="OQQ66_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1904570.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19504..20292)
                     /gene="ygiD"
                     /locus_tag="OQQ66_001599"
     CDS             complement(19504..20292)
                     /gene="ygiD"
                     /locus_tag="OQQ66_001599"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1904571.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20435..21196
                     /gene="zupT"
                     /locus_tag="OQQ66_001600"
     CDS             20435..21196
                     /gene="zupT"
                     /locus_tag="OQQ66_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1904572.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21254..21424)
                     /locus_tag="OQQ66_001601"
     CDS             complement(21254..21424)
                     /locus_tag="OQQ66_001601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904573.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(21684..22337)
                     /gene="ribB"
                     /locus_tag="OQQ66_001602"
     CDS             complement(21684..22337)
                     /gene="ribB"
                     /locus_tag="OQQ66_001602"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1904574.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22441..22589)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            22711..23001
                     /gene="ubiK"
                     /locus_tag="OQQ66_001603"
     CDS             22711..23001
                     /gene="ubiK"
                     /locus_tag="OQQ66_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1904575.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23285..23836
                     /locus_tag="OQQ66_001604"
     CDS             23285..23836
                     /locus_tag="OQQ66_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS1904576.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            23896..>25353
                     /locus_tag="OQQ66_001605"
     CDS             23896..>25353
                     /locus_tag="OQQ66_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1904577.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT         6360 a         6675 c         6259 g         6059 t
ORIGIN      
        1 gttttttccc ggtgtaatac atggtggttg agttcgccag cggcgatggg tagaagttct
       61 gtacctggtc gagacggaaa cgatgctttt tcagccacag cgccagattc accatatctt
      121 catcacgcgt accggggtgc gcggagatga aataggggat cagatactgc tctttacctg
      181 cctgttttga gtaagtatcg aacagctctt taaagcggtc atagctgccc atgcccggct
      241 tcatcatctt cgataacggc ccttcttcgg tatgttccgg ggcaatcttc agataaccgc
      301 cgacgtgatg ggtcgccagt tctttgatat agcgcggatc ttccacggcg atgtcataac
      361 gcacaccaga ggcaatcagg atctttttaa tgcctttcag atcacgcgca cggcgataga
      421 ggttgatcgt tggttcgtgg ttcgtgtcca tgtgcggaca aatatccgga taaacgcacg
      481 acaaacggcg gcaggtttgc tcagcgcgtg gcgatttgca gcgcaacata tacatgttgg
      541 cagttggccc accgagatcg gaaattacgc ccgtaaaacc tggaacggtg tcgcggatcg
      601 cttcgatctc attaatgatc gaatcttcgg aacggctctg aataatgcgc ccttcgtgct
      661 cggtgataga acagaaagag cagccgccaa agcagccacg cataatgttg accgaaaaac
      721 ggatcatttc gtaagccgga atacggacat tgccataggc cggatgtagc acgcgcttgt
      781 atggcagcgc aaaaacgctg tccatctctt cggtagaaag cggaatagca ggcgggttga
      841 tccacacata gcggtcgccg tgtttttgca tcaatgcgcg ggcacagcct gggttggttt
      901 cgtggtgcag aatacgcgaa gcatgggcgt acagcacttt atcgcccttc actttctcga
      961 aagaaggcag caacacgtag gttttttccc acggtttcgg gcgcggtggc tgcacggtta
     1021 cggctttggc ttcctgcttt ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt
     1081 caccatacgg atgcgggatt gggtcgattt ttccaggggt atcaagacgg gtggaatcca
     1141 cgccgctcca gccaggcagg gcctctttca cgataatcgc ggtattacgc acatcgcgga
     1201 tttcactaat cggctcgccc atcgccagac ggtgcgccac ctccaccagc ggacgctcac
     1261 cgttaccaaa catcagcatg tcggctttcg aatccaccag cacggaacgg cgcacggtat
     1321 cggaccagta atcataatgt gcggtacgac gcagactagc ctcaataccg ccgaggatca
     1381 ccggtacatc tttccacgcc tctttacaac gctgggtata aaccagtgtg gcgcgatccg
     1441 ggcgcttacc cgcgacgtta tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg
     1501 cggtataacg gttgatcatc gaatccatgt tgccagcagt aacaccgaaa aacagattcg
     1561 gtttacccag acgcataaag tcgtctttgc tgctccagtc cggctgggcg atgatcccga
     1621 cgcgaaagcc ctgcgcttcc agcatacgac cgcaaatcgc catcccgaag cttgggtgat
     1681 cgacatacgc gtcgccagta accaaaatga tgtcgcagct atcccagcca agttgatcca
     1741 tctcttcact agacatcggc aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg
     1801 gccaggagaa caggtcgcga tccggttgga tcagggagat agagctcatt ttgcttccag
     1861 aaatgataaa aaaataatca aaggccgggg attataagcc ggaacgaaag agaaatcgaa
     1921 aagtattcca tactcgccct cctcgggcga gtatgaagat tacggtaccg gattgactaa
     1981 cagttgccca atcgagccac ggtccgccat ttccagcgtc tgactgttga agtagaacgg
     2041 gaagtgcgcc caggaaggct gaccgaaata aacaagcagc tccacttgtc cgtcaaccca
     2101 aacggtatct ttccagcctc tgtcttccgg gaacggcatc gcaccattca cgttacggat
     2161 ctggaacatc acgccttcaa tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg
     2221 ttcccacgtt ccttgctgcg cggtgacatc aatacggttg acgtcccaca gttgtccatt
     2281 aatacccggg tcatcaccca gactgatatc gcgactgcga atcggcgaac cggccatgat
     2341 ttcagttggc agcaagcgca tcggaagact gtctgtgacc agcggcagaa ggccggttgg
     2401 gcgcagcgtt agcaccaggg tagaaaccag aatgctggat ggctcaaaga agccacgaat
     2461 acgatcaaca atgctcgccg cttcgccaca ggtgatcgac acttcatcac cgttgctcat
     2521 atccaccaga atctcgcggc gctcgcccgg tgccaacgaa agttgcttca ccgatacagg
     2581 agcaggcagg aatccctgat cgccagaaat cacatgtaac gggcgactat cgctcatctg
     2641 tagttgatag cgacgagagt tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga
     2701 gacttcaacg tacgggcttt gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc
     2761 gcttcccggt tcgttgtatt ctggcgtacc aaagttatcc agccgtttat cctggataat
     2821 gaccggaaaa tcatccacac cataatggtt ggggataggc agcgacttgc tgacttcatc
     2881 ttccaccagc cacattccgg caaggccgtt atagacctgc tgagccgtgc ggttgggagt
     2941 attggcgtga taccacagag tagctgcgtt ctggcgaatg ggcagtacgg gtgcccagtc
     3001 agcgtttggc gacatcatcc gtgccggacc gcccatcagc gggcctggta cctgtagccc
     3061 ggccaccgtc attgagacat tttctgtcag gcggttgctg taaataagct taacatcgtc
     3121 gcccttccag acgcggatag tcggccccag gtaacgacca ttgattcccc agaccgacgc
     3181 gcgtgtccct ggcgtaaatg accagtgcgc acgttgtaca gtcataaaca gcggttgccc
     3241 acggcgagat tcaagtagcg gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc
     3301 cttcagggga acagcgccgg cacaaagtgc aatccccgat gcctgaatga actgacgccg
     3361 actgagtgac atatttgctc catgtaaaac tggctaaagt aataccgtgc aggaaagtgt
     3421 tccccgcaaa aatacagatt gccgacttaa acttttccgg cggcttcgcg ttctgcgact
     3481 tctttatcga gctcggcgat tttttgttcc attatcgaac gacaatgggc agccagctca
     3541 cgaacctgat ctttgccata ctgactgacg tcaattggcg gcagcatttc gacaatcacc
     3601 agaccgttgt gcagtcgatt aagattaatc ttattcgaag ttgtagagac gcacacggga
     3661 ataatcggga cgcccgccgc aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg
     3721 ccgcgaccac ggctgcgggt tccttccggg aacatccaga tggaaatacg gcgttttttg
     3781 aaatgattca cgacttccgc aatggtgccg tgagctttag tgcgattgtt tctgtcgatc
     3841 aataagttgc cggttaacca gtacaactgc ccgaagaagg ggatccacag caagctcttt
     3901 ttacctaccg tcaccgtcgg cggttgcaca atgttcgatg ctgtcaccat gtcatagttg
     3961 ttctggtggt tagcgatata gatagcattg ccgtagcttt cagcatctgc aggtttgcgg
     4021 cactcaactt tcaggccgaa cagcggcgca agacggccaa acatatgccc aaaggtggcc
     4081 acatgtttcg ggttacgcgg gctgaaaagg cagtaaatgg agccgaatac acagactaag
     4141 atgctgtaaa tcacggtaat aataagacga aagatatata gcataacaac ctctgaaggt
     4201 ctgagagact ggcattgtac gtcacctgaa atcaggttag ggcattgtta aaagccgctc
     4261 tctggaaaag cgtattgtta atcgcaacgc atgaataaac aacggtttca agggaatttt
     4321 tatcgtttcc gggggttgaa tacgctgccg gatgacgact taacgtttca tccggcgttc
     4381 cttgcaagcg ggaggaaaca ggccctcccc ggcatattac tcttcgctat caccgctgct
     4441 ggcacggcga ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt
     4501 accgcggcgt ccacgttcgc cagtgacttt ctgtaactct tccgggcgca gtttaatttt
     4561 gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca attgcgccag
     4621 accatcttct ccacgcgcgg cttctgccga tggaatgttg ataatcttgt tgcctttgcc
     4681 cttcgacagc tgcggcagat cgcttaccgg gaacatcaac atacggcctg cctgagtgat
     4741 tgccagcagc atatcggaag catcttcaat caccaccggc ggcataacat gggcattttc
     4801 cggtaaggtg atcaaagcct tacctgcacg gttacgcgcc accagatcgt taaaggtgca
     4861 gacgaaaccg taacccgcat cggaagccat cagcagtttc tgatcgtcgc tttccatcag
     4921 catatggtca acggtcgccc caggcggcaa cgttaatttg ccggtgagtg gttcgccctg
     4981 accacgcgcc gacggcagcg taatcgggtc gatggcatag ctacgaccgg tggaatcaac
     5041 aaacactacc ggttggttgc tcttaccttt caccgccgct ttgaagctat cacccgcttt
     5101 ataattcagg cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc agcccatctg
     5161 cgacagcaca atggtgacag gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc
     5221 ttcttcgcgt tcctgcaacg gcgaacgacg atcgtcaccg taagcttgcg cgtctgcctg
     5281 cagttctttc ttcagcaggt tattcatttt acgctcggaa accaaaatgc cctgcaactg
     5341 gtcgcgctct ttttccagct cactctgctc accgcgaatc ttcatctctt ccagtttggc
     5401 aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg
     5461 cgacatcagc gccggtttcg gttcatcttc gttacgaatg atctcaatca cttcgtcgat
     5521 gttgagaaac gccaccagca aaccttccag gatatgcagg cgcttgagga ctttctccag
     5581 acgatagttc agtcggcggc gcacggtatc gcgacggaac accagccatt cggagaggat
     5641 ttccagcagg tttttcaccg ccggacgacc atccagaccg atcatattga ggttaatgcg
     5701 atagctcttt tccagatcgg tggtagcgaa gaggtggttc atcacctgat ccatatccac
     5761 gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg
     5821 cagatcgtca accatcggca gctttttgtt gcgcatttgc gcagcaattt gctccagtac
     5881 gcgcgcacct gaaacctgat gcggcaatgc gctgataacc accgcgccat cttctttctt
     5941 ccacaccgcg cgcatacgca ctgaaccacg tccgttctcg tagattttac ggatctcggc
     6001 gcgcgaagtg ataatttccg cttcagtcgg ataatccggc ccctgcacga tatccagcag
     6061 ctgatcgagc gtggttttcg gctggtcgat taatgcgatt gccgcctgag ctacttcacg
     6121 caggttatgc ggtggaatat cggtcgccat gccgacggca ataccggtgg tgccgttaag
     6181 caaaatgttt ggcagacggg caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt
     6241 tggcacccag tcagccgtcc cctgccccag ctcgctcaat agcagctcgg aatatttcga
     6301 caaccgggat tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca
     6361 gttcccctga ccatcaacca gcggataacg gtaagagaac ggctgcgcca tcaggaccat
     6421 cgcttcataa caggcgctat cgccgtgcgg atggtattta cccagtacgt caccgacggt
     6481 acgggccgat tttttaaatt tggcgctggc attcaggccc agttcagaca tcgcatacac
     6541 aatgcggcgc tgaacaggtt taagaccatc gccaataaac ggcaacgcac ggtccatgat
     6601 cacgtacatg gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc
     6661 tgccatatcg ctcattaatt ctgattcctc aacttattcg ccagccttca aaggggcaat
     6721 attgccggag atactacatt atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg
     6781 ccgccggacg tgctccgacg gcgataaagg catcaatgtg ccttgatata caactctttg
     6841 ctgtagactt gatcggcata gagggcagca caaggttgcg cccatgccac accactcggc
     6901 atgggggtcc gctccgggcg attcgagaag ttgaggttat gcgagtccag ggaagcctaa
     6961 agcatcaaag tagtcgactg taagtatacg gttcagccca gtttagctgt gtctccacca
     7021 gtgccccgcg cctcctgcca taagtgcggc ttgctcatgg atacccccga gacggcttac
     7081 ggcgcaccgg ataataaatt tcgcgtatac atggtattcc ttaccggaga aattataagc
     7141 ccaacaacaa tacgcctgtg gcattgttcg ctcaaactta tcgcgagtga tttggctcac
     7201 actccggtaa agattcccca tcacgctaac ggcacataaa catccgtttc cagtcgctgt
     7261 tctgtcatac cttcggcaag attggtgtag tgaaacagaa tcggtgcttt acgcattttc
     7321 tcgccgcttg caggcagcca gtggcgaatg atgccccata ccgtgtgtga aatatcgtcc
     7381 agctcgccaa cgtggcgggc cacggcataa cgtccaccgg taagttcacc attgctcaca
     7441 ccataacgat tatcgggaat cggttcgcta acgctgccgc agatatcaaa gcgaaacgct
     7501 ttcggtgcgg tggtggcagg gtcgtcccag gcgacgccaa aagtctggct ttggttaaca
     7561 ggagaaagcc ccgtctcttt acgccacatg ataaatttcg ccacactgta gttgagcaac
     7621 tcagggctac agcggtgcgg caacatcgcc acagggattg atgggaaatc gataatgttt
     7681 acatccagtg tcaggtttgt cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc
     7741 cgggagcggt aacaattacg gatttacttt gcggatctgt ttgacgtcaa tttcaacaga
     7801 attccagtct ttatcgactt ccccctgaat ctcaaccgta tctttcggcg tcaccgtcac
     7861 accgttccag cgtttgtggt cgatatcaac attgatagta ccgctggcat ctttaaacac
     7921 gtagagatcg tcagagatgc gttcaacgat attgccgcgc agggttaccc aggtgtcgtc
     7981 acgccgggat tttgcgcttt ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc
     8041 ggcctgactt tgcgttgccg atgggccaga aaaaccgccc tgctctgctg ccatcaccgg
     8101 tgcactgcac agggccatta ctgcgattac tgctgcgaat tttttcatgt ttattactcc
     8161 ctttaatgtc tgtttccgag catttaacaa gatagtcctt aacaacttct taagggaaaa
     8221 aaataaaatt taatgctgta cagagcgcgt tacaacacgg tttactggca gcaaatacgg
     8281 ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa gatgacatgc tgattggcga
     8341 cggcatcaaa actggcctta gtaaaatggg ttttagtgtc gactggttta cacaaggtcg
     8401 tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg gtgatcctgg atttaacctt
     8461 accaggcatg gatggtcgcg atattttgcg cgaatggcga gagaaaggtc agcgtgagcc
     8521 ggtactgatc ctgaccgcgc gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg
     8581 agctgacgat tatctgtgta aaccttttgc gttgatagaa gtcgccgcca ggctggaggc
     8641 tctgatgcgc cgaaccaacg gccaggccag caacgagctg cgccacggta acgtcatgct
     8701 cgaccccggc aaacgtatcg ccacgctggc tagcgaaccc ttaacactga aaccaaaaga
     8761 atttgccctg ctggaattac tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat
     8821 tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt
     8881 gcatcatctg cgacgcaaac tcggtagtga ttttattcgt accgtgcatg gtattggcta
     8941 cacattaggt gagaaatgaa atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta
     9001 atctttttaa ttctggcctc ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa
     9061 acaacggata acgtcgatga attgttcgac acccaactga tgctgtttgc caagcggtta
     9121 agtacgctcg atctcaacga aatcaacgcg gcggatcgca tggcacagac gccaaataaa
     9181 ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg ccatctttac ccacgacggc
     9241 agaatggtcc ttaatgatag cgataacgga gaagatattc cctatagcta tcaacgggaa
     9301 ggttttgctg acgggcaact ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc
     9361 tcacctgatg gcaaatatcg catcgttgtt ggccaggaat gggaataccg tgaagacatg
     9421 gcgctggcga ttgttgccgg gcaattgatc ccgtggctgg tcgcactgcc gattatgtta
     9481 atcatcatga tggtactact gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca
     9541 ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa
     9601 gtgcgaccac tggttgagtc gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt
     9661 cgagaacgac gctttacctc cgatgcagct cacgaactac gtagcccgtt aacagcgctg
     9721 aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc cgcaggcacg gaaaaaagca
     9781 ctgctccaat tacattccgg gatcgatcgc gctactcgtc tggttgatca actgctcacg
     9841 ctatcgcggc tggactcact ggataacctt caggacgtcg cggagatccc gcttgaagat
     9901 ctcctgcaat cgtcggtgat ggatatttac cacacggcgc agcaggcgaa aattgacgtg
     9961 cgactgacac tcaatgtcca gggcatcaaa cgcaccggac aaccgctatt gctaagtttg
    10021 ttggtgcgaa atttgctgga taacgccgtg cgttacagtc cacagggcag cgtggtggac
    10081 gtcacgctga atgccgataa tttcatcgtg agggataacg gccccggtgt gacaccagag
    10141 gcactggcgc gaattggcga acgcttctat cgcccctccg gacaaaccgc taccggcagt
    10201 ggacttgggc tatcgattgt ccagcgaatc gccaagctgc atggcatgaa tgttgagttt
    10261 gggaatgcgg aacaaggtgg atttgaggcg aaagtaagct ggtaagggta agacttttgc
    10321 taaattcgca aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact
    10381 atagtcggca aacgtctcac cttgaggtta aaaaatgagc aacattctga ttatcaacgg
    10441 cgcgaaaaaa ttcgcccact ccaatggtca actgaatgac accctgaccg aagtcgcgga
    10501 tggcacactg cgcgacctcg ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga
    10561 tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg
    10621 ctggtggatg ggcgcgccgt ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg
    10681 tcacgggacg ctgtatgcca gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg
    10741 ttccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcaccaat
    10801 ggaagccttc accgaaaaag atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct
    10861 gccgttccat aaagcaaacc aattccttgg catggaaccg ctgccgacat ttatcgctaa
    10921 tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt
    10981 ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat
    11041 cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc
    11101 taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga
    11161 ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca
    11221 gtgggaaagc atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag
    11281 cgaagccgta aaaggtgacg tgctggagat gaatattcgt attctgcagc cagggattta
    11341 atcctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat
    11401 ctccgccatg tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc
    11461 cagtagcatg tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg
    11521 caccagacgg cgagtgttcg gatcaagcgt ggtttcgcgc aattgcatcg ggttcatttc
    11581 ccccagacct ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag
    11641 tacgccctct ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg
    11701 gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt
    11761 tacaaacaaa gcgcagagca gcgtggcaat gtgcagacca tcggagtcag catccgccag
    11821 gatacagatc ttgccgtaac gaagctggct caaatcgtcg ctgtcaggat cgataccgat
    11881 cgctaccgaa atatcgtgca cttcctgcga agccagcact tcgtcggaag agacttccca
    11941 ggtgttaagg atcttacctt tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg
    12001 cttggcagat ccgcctgcgg agtcaccttc cacaagaaac agttcggtgc ggttaaggtc
    12061 ctgcgcggta cagtcagcca gtttgccagg cagcgccgga ccgctggtca gctttttacg
    12121 caccactttt ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag
    12181 caactccgcc gcctgaacgt tctggttcag ccacaggata aaggcatctt tcaccacgcc
    12241 agaaacgaat gccgcgcact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg
    12301 cggatcctgc atttttactg acagcacata ggcgcagcga tcccagatat cttccgccga
    12361 cagctttaca ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccag
    12421 caggccctga cgcagaccat taacatgggt accgccctgc atcgttggga tcaggttgac
    12481 gtagctttcg gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccaccgc
    12541 ttcagtatcg ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt
    12601 taccgcttcc gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt
    12661 gttgatctca tctttaaagg taatctcaac gccggggcac agtactgctt tggctttcag
    12721 cacatgcgtc aggcgtgaaa cagaaaaacg cgggctatcg aagaaggttt catccggcca
    12781 gaaatggacg ctggttccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg
    12841 caccttttcg ccattttcaa aggcgatgtt ataaatctga ccatcgcggc gcacgttaac
    12901 ttctacgcgc ttcgacaggg cgttaaccac cgaaatcccc acgccatgca ggccgccaga
    12961 gaactggtaa tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc
    13021 aaccgccggt acaccctctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat
    13081 aacttctaac gactggtcag catgtaaaat aacgtccacg cgttttgcgt gacccgccag
    13141 tgcttcatcc acactgttat caatgacttc ttgccccaaa tggttagggc gagtggtatc
    13201 ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc
    13261 agcgttataa gtttgcgtca tggtttaagt tagtaattcg agttgatcgt cagagatggt
    13321 gcagaccaag aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt
    13381 tgccgccttc tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat
    13441 ccagcacttc atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt
    13501 caatctgcat gactttaaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg
    13561 tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg
    13621 ggtttaccac cactgcgggc agcataaaac attgtgacaa ccaggtggcg taatatcccc
    13681 ccagtgacga accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca
    13741 gcagctctgc cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag
    13801 ggtgatgttc cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt
    13861 tgaaaccgtg taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg
    13921 gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc
    13981 gagagtacgc cagccgggcg cgatggtatc cagcgtaaag ttggaacagt gcggcttaaa
    14041 ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc
    14101 ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc
    14161 cagttcgccc gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg
    14221 atgatgatgc agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag
    14281 ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag
    14341 caacaggatt tgccattgct caccaataaa cacgcgcttc gccggggaga tacccgcatc
    14401 ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg
    14461 cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg
    14521 cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc
    14581 cagcaccgcc tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc
    14641 aaacaggtga gtatcggtaa tttgtaaaat cctgactctg gcctcaccag ccagaggaag
    14701 ggttaacagg ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc
    14761 catcgctcca tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg
    14821 cttttcgtcg cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa
    14881 aatctgctgg cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat
    14941 tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccaaggtagt
    15001 gtatcgggtc gattccacag tcgtcagccg atattgtgcg tttgccacct gatagcttac
    15061 agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc
    15121 gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt
    15181 ttttaacgct tggtggtgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc
    15241 gattttcccc tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat
    15301 atcttcgttt tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc
    15361 caccataatt gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg
    15421 tttggtccgt ttgactatca gccccgcctc ttcaatcgct tcgcgacggg caacatcttc
    15481 cacactttca ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcgct
    15541 agtgtcgtac gcggcaattc gaatctgctc aatcagcaca acttcatcac gcactgggtc
    15601 aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgca cctcatgact
    15661 catttgcccg ttgaatagac tatgacgaaa tctataaaga tctaatgaaa aaaagccgcg
    15721 ataaagtgtt tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt
    15781 gtctggctta agcattgtta atgtcctggc actaatagtg aattaaatgt gaatttcagc
    15841 gacgtttgac tgccgtttga gcagtcatgt gttaaattga ggcacattaa cgccctatgg
    15901 cacgtaacgc caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt
    15961 ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt
    16021 cttatcggcc tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa
    16081 gtttatcagc aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat
    16141 gctgcctttg aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt
    16201 gcagattaca cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc
    16261 agtgcgtccc tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg
    16321 ctgcaggaaa aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg
    16381 atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat
    16441 acacaggcac aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac
    16501 gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg
    16561 gcgaacgaag tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc
    16621 accggtaact actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa
    16681 ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag
    16741 gcacgcttga gccaggacct ggcgcgcgag caaattcgcc aggcgcagga tggtcactta
    16801 ccgacgctgg atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa
    16861 actcgtggtg ccgctggtac ccagtatgac gacagcaata tgggccagaa caaagtgggc
    16921 ctgagcttct cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggcc
    16981 cagtacaact ttgttggtgc cagcgagcaa ctggaaagcg cgcatcgtag catcgtgcaa
    17041 accgtacgtt cctccttcaa caacattaat gcatctatca gtagcattaa cgcctacaaa
    17101 caagccgtag tttccgctca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt
    17161 acgcgtacca ttgttgatgt gttggatgca accaccacgc tgtacaacgc taagcaagag
    17221 ctggcaaatg cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt
    17281 acgttgaacg agcaggatct gctggcactg aacaatgcgc tgagcaaacc ggtttccact
    17341 aatccggaaa acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg
    17401 cctgatagcc cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt
    17461 cataaccctt tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaagac
    17521 aacgtaaaga tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag
    17581 ccatcctcta ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca
    17641 aaatccatac gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc
    17701 gctctcgcgg ttgccactgt ttttatgctg gctagctgtg aaaagagtga tgaaacagtg
    17761 tctctctatc aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt
    17821 accaccgcgt acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc
    17881 cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc agcccaggct
    17941 ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg
    18001 ctgatggccg gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag
    18061 ccgctgttct cctcgaaaaa cccagccagc ccggcttacg gtaaatatac cgacgcgacg
    18121 ggtaaaaact atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacagccatg
    18181 gcaccaaaac cggcgaccac tactaccgtt acccgtggcg gttttggtga atctgttgcc
    18241 aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat gggtggctga
    18301 taccgatgga aagagtcagt attaccgagc gcccggactg gcgtgaaaaa gcccacgaat
    18361 acggtttcaa ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca
    18421 agttgcccct cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt
    18481 gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc
    18541 caaaacacac ctggagtttt gtgcgtcagt catggctgac tcaccagcca tcgctttatt
    18601 cgcgtcttga tctggcatgg gatggcactg gcgaacctaa actgctggaa aataacgccg
    18661 atacaccaac gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc
    18721 ttaacgccgg taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaagctaa
    18781 tcgatcgctt tgtcgagttg cgtgaacagt atggcttcca gttgctgcat ctcacctgct
    18841 gtcgcgacac ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcgacggaag
    18901 ctgaaatcgc taccgagttc ctctacattg acgatatcgg gttaggtgaa aaaggtcagt
    18961 tcacggattt acaggatcag gtaatttcca acctgttcaa gctgtatcca tgggaattta
    19021 tgttgcgtga gatgttctcc accaagctgg aggatgcagg cgtacgctgg ctggaaccgg
    19081 cgtggaagag cattatctcc aacaaagcgc ttctaccgct actgtgggag atgttcccga
    19141 atcacccgaa cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat
    19201 atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca
    19261 aaaccattga agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc
    19321 atccgttacc gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc
    19381 aacccgccgg aattggcatt cgtgaagacc gtgcattgat cacccaggat atgtctcggt
    19441 tttatccaca tatttttgtt gagtaatcaa tgatgccgga tggcattcgc catccggtaa
    19501 ttgttaacct atctgcaccg acagcatact caggctgccc atttctatac catcgaccgg
    19561 aatggtaatt ggctcctgcc catcccacgc acctaagaca tacaacaacg gcagatagtg
    19621 ctccggcgtt gggttcgata acgcgccacc ttcatggtcg aggtaattca ccagaggatg
    19681 ctgctctacc gccccttgcc acgtcagatt cgctttcaca tactcattaa acgacatcgc
    19741 ccaaggatat ggtgaactat caccgtgcca cttcactgta cgcaggttat gcaccacgtt
    19801 gccactggcg accaacatta ttccttcatc tcgcagcgct gccagtttgc gccccatttc
    19861 gaaatgccag gcggcaggtt tgctactgtc gatactcaac tgcaccatcg ggatatcggc
    19921 gtcaggatac atcttaatca gcacgcccca ggaaccgtgg tcaaagcccc tggcttcttt
    19981 atccagcgtc accgggaccg gcgctaacag ctcaaccaga tgctgtgcca gcacaggcga
    20041 acccggagca ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat
    20101 cgtgggcggc gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac
    20161 caccacaatc gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata
    20221 caaattatct tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg
    20281 tgttgaagac atgatgatat ccttaactaa gggtgtcatt ttgatatcct cacaatacgc
    20341 ttgttcggcg gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat
    20401 ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat
    20461 actggcgggg gcagccacgt ttattggcgc gtttctcggc gttctcggac aaaaaccctc
    20521 gaaccgctta ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt
    20581 aatggaaatg cttcctgccg cactagcagc tgaaggaata tcgcctgtgt tgggttatgg
    20641 aatgtttatc ttcggtctgc ttggctactt tggcctggac cgcatgttgc cgcatgcgca
    20701 tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca agcgcacagc
    20761 cattctgctc actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt
    20821 cacggcgagc agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa
    20881 tatccctgaa ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac
    20941 cgcgattctg tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg
    21001 gttaatcctc ggtagcatga tttccccggt ggtcatggcg gcggttgcag gaattatggt
    21061 ggcgctctcg gttgatgaat taatgccgct cgccaaagag attgacccta ataataaccc
    21121 cagctatggc gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac
    21181 ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc acatggacaa cttctgccag
    21241 cttcacgaaa gccttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt
    21301 tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa taacgtttca
    21361 aatgcaggaa ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa
    21421 acatccttta cccttaatat ttaacaatac taaacattta gcgcataaat ttcacatatc
    21481 ctttttcgga tatattcata tggtcgcata ataacggaac aattatatga ttttgatcat
    21541 aatgaaaaag aatgcctgat attaatgaaa ttttctcata gaaagagaag cggaattaac
    21601 atcttgcata gcagggcaaa ataaaaaccg aagccagcta caacttcggt ttcttgatta
    21661 aggcagtaat taagcagcgg ttttcagctg gctttacgct catgtgcctg acggtatgcc
    21721 accaggtctt caatagtcac gagcgccata ttgtgtttat tggcaaactc aatgcactct
    21781 ggtgcacgcg ccatcgtgcc atcgtcatta gtcagctcac acagcacacc agccggttta
    21841 aagcctgcca gcgtcatcaa atcaatagtc gcttcagtat gaccgccacg cgtcagtaca
    21901 ccacctgcct gcgcgcgaag tgggaaaacg tggcccgggc gattcagatc agaaggtttt
    21961 gcgccatcgg caatcgctgc gcgaacggtc gtaatacgat cagcggcaga aacaccggta
    22021 gtcacacctt cagccgcttc aatggtcacg gtaaaaccgg tgccataggc gctggtgtta
    22081 ttttctacca tcattggcag atcaagttgt ttacggcgat cttcagtgat gcacaggcaa
    22141 acaataccgc taccgtggcg aatggtcagc gccatctgct caacggtcat ggtttctgcc
    22201 gggaagatca tatcgccttc gttttcacgg tcttcatcat caagcaccat tacaccgcgt
    22261 ccttcacgca gcgcagccag tgcattttca acacgttcga aaggcgtacc aaaagaggaa
    22321 agtagcgtct gattcatggt aaaaaaaact cactaaaatt atggttacca gaatcagggc
    22381 agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc ccgacagaat
    22441 cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg gatctgctgt
    22501 cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt accgccggtg
    22561 gggaatttcg ccccgccctg agaataagcg gattcactat aacgctaatg attagcggca
    22621 gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac tacaatggta
    22681 ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc
    22741 gctcgccaag ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga agatgtggag
    22801 aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa
    22861 gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc gctgcttgaa
    22921 caaagaatga gcgaactgga aaatcgcagt actgagatta aaaagcagcc agatccagag
    22981 actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat gaattaatct
    23041 tctaatttac agtgctttct attaccttat cgcttaattc cattcagaat atcaccacaa
    23101 aaatgaatga gtgcaattga aaatattatt gttatgtatt tttttaagtt ttacttaatc
    23161 catatttaga taaactaatt tcataaaacc cgaaaagtaa aatataatat ttgaatgatt
    23221 agcaatcctt aaataacagt tactaatcat tcattttcta cttatattta tataaaggaa
    23281 tattatgtct gcttttaaaa agtcacttct tgttgctggc gtagcaatga ttctttccaa
    23341 taacgtattt gctgacgaag gtcacggcat tgttaaattt aaaggtgaag ttatctctgc
    23401 tccatgttca attaaaccag gtgacgaaga cttaactgta aatctgggtg aagtagcaga
    23461 taccgttctg aaaagcgacc agaaatcttt ggctgagcct ttcactattc acctgcaaga
    23521 ctgcatgttg agccagggtg gaaccactta ttcaaaagcg aaggttactt tcaccacagc
    23581 gaatactatg acgggtcaga ctgacctgct gaaaaacacc aaagaaactg aaatcggtgg
    23641 tgccacaggc gttggcgtac gtattctgaa tagccagagc ggcgaagtga ctttaggtac
    23701 tccggtagta attacattca acaatactaa cagctatcag gaactgaact tcaaagttcg
    23761 tatggaatcc ccgggcaaag atgctacccc gggcaacgtt tacgctcagg ctgactataa
    23821 gattgcttac gagtaatcac attaggccgg gttcgcccgg ccacatcatt aacggattaa
    23881 tgataagtgg aacagatgta taaaaaatta aaattaacca caataagcga attgattaaa
    23941 aatatttatt gttcattatc cgttctcatc attggttgtg cgtcagctta tgccgttgaa
    24001 ttcaacaaag atttaatcga agccgaagat cgtgaaaacg ttaacctttc ccaatttgaa
    24061 actgatggcc aattacccgt aggtaaatac tcattaaacg ctattattaa taataaaagg
    24121 acgccaatcc atctggacct gcaatgggta ttaattgata accaaactgc agtttgtctg
    24181 actccagagc aattaacatt attaggcttt actgatgaga ttattgaaga ggctcagcaa
    24241 aacctgatcg atggttgtta ccctatcgaa aaagaaaaac aaattacaac ttatctcgat
    24301 aaagggaaaa tgcaattatc catatctgca cctcaggcat ggttaaaata caaagatgca
    24361 aactggacgc ctcctgaact ttgggatcat ggtattgctg gggcatttct tgactacaat
    24421 ttatatgcct ctcattatgc accacatcag ggcgataatt cacaaaatat aagttcctat
    24481 gggcaggctg gggttaatct tggggcctgg cgcctgcgta ctgattacca gtacgatcag
    24541 tcatttaaca atggcaaaag ccaggcgaac aacctggatt ttccgcgtat ttatttgttt
    24601 cgcccaatcc cagcaattaa tgcaaaacta actataggtc aatacgatac tgaatcctct
    24661 attttcgact ctttccattt ttctggcgtt tcgttgaaaa gcgatgagaa tatgttgccg
    24721 cctgatctac gtggttacgc accgcaaatc acgggtgttg cacaaacgaa tgcaaaggtc
    24781 actgtctcac agaacaaccg tattatttat caagaaaatg ttcctcctgg cccatttgct
    24841 attactaatt tattcaatac attacagggg caactggacg tcaaggttga agaagaggac
    24901 gggcaagtca cgcaatggca agttgcatct aatagtattc cttatctgac gcgtaaaggg
    24961 cagattcgct acaccactgc tatgggtaaa ccgaccagcg ttggtggtga ttccttacaa
    25021 caacccttct tctggactgg tgaattctca tggggttggc tgaacaatgt atccctgcat
    25081 ggtggttcag ttttaacaaa ccgtgattat caatctctgg ctgccggcgt tggctttaac
    25141 cttaactcat tgggttcatt atcttttgat gtcacacgat ctgatactca gttgcataat
    25201 caggataaag aaacgggtta tagctaccgc gctaactatt caaaacgttt tgaatctacc
    25261 ggtagccagc tcactttcgc tggttaccgt ttctctgata aaaactttgt gacaatgaat
    25321 gaatatatca atgacactaa ccattacacg aat
//
LOCUS       DAJVTY010000043        25302 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVTY010000043 DAJVTY010000000
VERSION     DAJVTY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25302
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.043"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..184
                     /locus_tag="OQQ66_001606"
     CDS             <1..184
                     /locus_tag="OQQ66_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCS1904578.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
     gene            254..1030
                     /gene="yaaA"
                     /locus_tag="OQQ66_001607"
     CDS             254..1030
                     /gene="yaaA"
                     /locus_tag="OQQ66_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1904579.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1183..1479)
                     /locus_tag="OQQ66_001608"
     CDS             complement(1183..1479)
                     /locus_tag="OQQ66_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1904580.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="OQQ66_001609"
     CDS             complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="OQQ66_001609"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1904581.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="OQQ66_001610"
     CDS             complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="OQQ66_001610"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1904582.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="OQQ66_001611"
     CDS             complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="OQQ66_001611"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1904583.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6401..6520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="OQQ66_001612"
     CDS             complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="OQQ66_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1904584.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6735..7421)
                     /locus_tag="OQQ66_001613"
     CDS             complement(6735..7421)
                     /locus_tag="OQQ66_001613"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1904585.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7821..7961)
                     /locus_tag="OQQ66_001614"
     CDS             complement(7821..7961)
                     /locus_tag="OQQ66_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904586.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8057..8773
                     /gene="arcA"
                     /locus_tag="OQQ66_001615"
     CDS             8057..8773
                     /gene="arcA"
                     /locus_tag="OQQ66_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1904587.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8833..10185)
                     /gene="creD"
                     /locus_tag="OQQ66_001616"
     CDS             complement(8833..10185)
                     /gene="creD"
                     /locus_tag="OQQ66_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1904588.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10243..11667)
                     /gene="creC"
                     /locus_tag="OQQ66_001617"
     CDS             complement(10243..11667)
                     /gene="creC"
                     /locus_tag="OQQ66_001617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1904589.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11667..12356)
                     /gene="creB"
                     /locus_tag="OQQ66_001618"
     CDS             complement(11667..12356)
                     /gene="creB"
                     /locus_tag="OQQ66_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1904590.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12369..12842)
                     /gene="creA"
                     /locus_tag="OQQ66_001619"
     CDS             complement(12369..12842)
                     /gene="creA"
                     /locus_tag="OQQ66_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1904591.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13053..13922
                     /gene="robA"
                     /locus_tag="OQQ66_001620"
     CDS             13053..13922
                     /gene="robA"
                     /locus_tag="OQQ66_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1904592.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="OQQ66_001621"
     CDS             complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="OQQ66_001621"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1904593.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14618..15139
                     /gene="yjjX"
                     /locus_tag="OQQ66_001622"
     CDS             14618..15139
                     /gene="yjjX"
                     /locus_tag="OQQ66_001622"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS1904594.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="OQQ66_001623"
     CDS             complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="OQQ66_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1904595.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="OQQ66_001624"
     CDS             complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="OQQ66_001624"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1904596.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17788..19455
                     /gene="ettA"
                     /locus_tag="OQQ66_001625"
     CDS             17788..19455
                     /gene="ettA"
                     /locus_tag="OQQ66_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1904597.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="OQQ66_001626"
     CDS             complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="OQQ66_001626"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1904598.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21015..22397)
                     /gene="radA"
                     /locus_tag="OQQ66_001627"
     CDS             complement(21015..22397)
                     /gene="radA"
                     /locus_tag="OQQ66_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1904599.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22446..23414)
                     /gene="serB"
                     /locus_tag="OQQ66_001628"
     CDS             complement(22446..23414)
                     /gene="serB"
                     /locus_tag="OQQ66_001628"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1904600.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23520..24164
                     /locus_tag="OQQ66_001629"
     CDS             23520..24164
                     /locus_tag="OQQ66_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1904601.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24192..25208
                     /gene="lplA"
                     /locus_tag="OQQ66_001630"
     CDS             24192..25208
                     /gene="lplA"
                     /locus_tag="OQQ66_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS1904602.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
BASE COUNT         6062 a         6992 c         6290 g         5958 t
ORIGIN      
        1 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
       61 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      121 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      181 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      241 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      301 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca
      361 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      421 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggcgaatgc
      481 ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt
      541 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
      601 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
      661 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
      721 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
      781 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
      841 gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg gtctgatgag
      901 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
      961 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1021 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1081 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1141 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1201 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1261 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1321 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgattgc ctatctgtaa
     1381 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1441 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1501 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     1561 atcctaatct ctaagttacc gcatttattg agaatatctc ctttttccct gtcgtttttc
     1621 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     1681 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     1741 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     1801 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     1861 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
     1921 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     1981 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2041 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2101 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2161 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2221 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2281 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2341 cgagttagct gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt
     2401 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2461 caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     2521 cactttcaca ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     2581 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     2641 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     2701 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     2761 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     2821 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     2881 gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     2941 gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3001 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3061 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag cgaacaaggt
     3121 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcaaccg cctgccgcgc
     3181 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3241 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3301 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc
     3361 cgttgagact ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac
     3421 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3481 tgccacgttg tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc
     3541 cagcaaacga gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc
     3601 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt
     3661 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
     3721 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     3781 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt
     3841 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     3901 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     3961 caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4021 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4081 cattaccatc cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa
     4141 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4201 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg
     4261 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
     4321 gaatcaatag cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg
     4381 tataacccat ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact
     4441 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac
     4501 cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     4561 catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
     4621 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt
     4681 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     4741 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
     4801 ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg
     4861 agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac
     4921 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     4981 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5041 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5101 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5161 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
     5221 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt
     5281 cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5341 cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgccg accattcctt
     5401 tcatccccgg accagaaacg ctgaacattg ccatattatt cagattggaa atgcctttga
     5461 ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc
     5521 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     5581 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     5641 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     5701 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     5761 cgttacgtcc aagtaccacc agttcgcctt tttcattacc ggaggtgaaa cctgccatca
     5821 acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat
     5881 cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg
     5941 taacgttgtg accacgagct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6001 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat
     6061 gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg
     6121 ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6181 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6241 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6301 tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6361 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaag cccgcactgt
     6421 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
     6481 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
     6541 atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
     6601 taaaatttta atttactcac ggcaggtaac cagttcagaa actgctatca gacactcttt
     6661 ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact
     6721 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
     6781 attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt
     6841 atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc
     6901 agttgatgtt ggtctgccgt tgtgtcactt ttcgtcggtt gttgtattaa tgttgctaat
     6961 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
     7021 ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg
     7081 cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcagcacc
     7141 agttctactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca
     7201 gtgaaatcga catcgtgtaa cgattcaacc agtgtcggga aaactttaat attatcaata
     7261 atatcaccag atccatgtgc gacccagcga gtggctggct ccagatgtgc ctgactatcg
     7321 acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt
     7381 tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact cttcttgacc
     7441 aaatgaccgc gaacagggtg atcactgtca acttattaca cggtgcgaat ttacaaatcc
     7501 ttaacgtaag tcgcagaaaa agccctttac ttatcttaag taaggctaaa ctatttcctg
     7561 actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta cttaggataa
     7621 ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa
     7681 ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca
     7741 aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt
     7801 gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt
     7861 gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg
     7921 atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa
     7981 tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc
     8041 aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac
     8101 acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga
     8161 tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat
     8221 caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt
     8281 tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat
     8341 cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg
     8401 caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga
     8461 aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga
     8521 tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa
     8581 cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa
     8641 accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac
     8701 gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga
     8761 tctggaagat taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt
     8821 tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt ctttactggc
     8881 tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc
     8941 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
     9001 cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctgttgcg
     9061 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
     9121 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
     9181 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
     9241 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
     9301 cgcccagtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
     9361 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
     9421 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggtag
     9481 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aagggcattt cgctattacg
     9541 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
     9601 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
     9661 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
     9721 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
     9781 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
     9841 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
     9901 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
     9961 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
    10021 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
    10081 ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc
    10141 accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
    10201 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
    10261 aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
    10321 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
    10381 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
    10441 actaccggta tccagcacct taagggcgac gtgttcctga tccacttcgg cgcttagcgt
    10501 tatgcgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
    10561 ctccagtaac gccggttcag cagcaacatt aacctcagtg ggcataacat gtagagtgat
    10621 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac
    10681 atcaacaaca gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
    10741 ttctaccaat gcctgcatac gcgcattttg cgtcagaatg ttgtcggtaa aacgagccac
    10801 cacttccggt ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
    10861 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
    10921 cttcacgcgc atactttcca gcgcctgcgc aagcttacgc aactcgctac tgccgagttc
    10981 aggaagagga acgggcttat tgtcagtgac ggaatcggca tagcgagtga gccgggcaat
    11041 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccagcaaaat
    11101 ggcgctggcc cataaaattc gccgctcgct acgcttaatg accggagcca tcgccgcgtt
    11161 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac
    11221 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
    11281 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt
    11341 tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat attcattgcg
    11401 cactttgtta atgccaccga tattggcgcg aaacggacga tgttgtagct gattaaacgc
    11461 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
    11521 cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
    11581 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
    11641 cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga
    11701 tgagtattaa tcggtgaaag atcggggttg atggcacgca gcttggcatg caacgtttta
    11761 atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatc
    11821 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
    11881 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
    11941 aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc
    12001 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
    12061 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
    12121 agtacaggta acgccggatg gagcgccagt aactggctgc acaattcaaa gccgctgata
    12181 tcaggcaaac caacgtcgag aatcatgacg tcgggaacca gctggcgagc tttatccagc
    12241 accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
    12301 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgtataaat
    12361 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
    12421 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
    12481 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
    12541 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
    12601 gaaatagccg catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt
    12661 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
    12721 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
    12781 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
    12841 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
    12901 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta
    12961 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
    13021 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
    13081 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
    13141 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
    13201 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
    13261 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
    13321 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
    13381 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
    13441 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
    13501 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
    13561 acgcgccgac cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata
    13621 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
    13681 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
    13741 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
    13801 tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
    13861 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
    13921 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
    13981 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
    14041 tacgcagacg taagcggcgc gctgcccatg ctggtaatcc gagaatcgta ctcaccagac
    14101 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
    14161 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
    14221 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
    14281 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
    14341 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
    14401 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
    14461 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
    14521 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
    14581 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
    14641 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
    14701 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag
    14761 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
    14821 ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt
    14881 ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc
    14941 ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac
    15001 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    15061 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    15121 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac
    15181 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
    15241 acacctcttc cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    15301 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
    15361 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    15421 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    15481 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    15541 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta
    15601 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    15661 cgtggcgctc atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc
    15721 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    15781 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    15841 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
    15901 actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc
    15961 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
    16021 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    16081 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
    16141 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    16201 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
    16261 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta
    16321 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg
    16381 cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg
    16441 gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
    16501 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
    16561 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg
    16621 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
    16681 cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc
    16741 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
    16801 attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa
    16861 atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
    16921 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    16981 caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    17041 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    17101 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    17161 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
    17221 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    17281 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    17341 attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    17401 cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat
    17461 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    17521 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    17581 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    17641 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    17701 tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc
    17761 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    17821 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    17881 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt
    17941 atggcaggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    18001 ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    18061 gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    18121 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    18181 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta
    18241 cgtctgccgg actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta
    18301 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    18361 cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc
    18421 gtggtggcga ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    18481 cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct agagcagaaa
    18541 gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    18601 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    18661 gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg
    18721 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    18781 tcctacggtg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc
    18841 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt
    18901 caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcatcggtt
    18961 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    19021 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    19081 aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt
    19141 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    19201 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    19261 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    19321 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    19381 gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag
    19441 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca
    19501 aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc
    19561 atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaatag
    19621 ccttagtatc cgcaatttcg tagcgggtca cccgtgctga ttcttttaaa ccttgtttaa
    19681 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc
    19741 ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac
    19801 gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    19861 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    19921 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    19981 acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca
    20041 gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac
    20101 tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa
    20161 tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact
    20221 ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    20281 accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca
    20341 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    20401 atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    20461 cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt
    20521 gctccacaca tcccagccgt gcggatacgg ctccatgccc tcctcgttga aagcatgaat
    20581 gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt
    20641 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    20701 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    20761 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa
    20821 ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt
    20881 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    20941 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    21001 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    21061 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    21121 cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg
    21181 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    21241 cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc
    21301 acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg
    21361 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    21421 ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    21481 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    21541 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    21601 tcattcaccg cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca
    21661 tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc
    21721 gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag
    21781 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    21841 tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg
    21901 gcaatcaggc agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    21961 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac
    22021 agcgttttca tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc
    22081 gcacctgggt taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg
    22141 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    22201 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    22261 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    22321 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    22381 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg
    22441 agcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    22501 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    22561 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    22621 gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg
    22681 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    22741 gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg
    22801 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag
    22861 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    22921 agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    22981 tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat
    23041 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    23101 gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt
    23161 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    23221 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    23281 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    23341 ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag gtcgcaccag
    23401 gtaatgttag gcattaagga tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    23461 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    23521 tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg
    23581 ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac cagcgacaac
    23641 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg ctgaacgttg
    23701 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    23761 taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    23821 cacgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcgg
    23881 gcggctattt taaccagcag attgtcgaac caattgccgg gaaaaacgga ccgctcggct
    23941 atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    24001 ccactaacat tttacgcctg atgttgctgc tttcactggc aatcggcgta gtgctgaccc
    24061 gcacgctgct acagggtaaa cgtacccgct ggcagcaatc gcccttcctg ttaaccgcca
    24121 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    24181 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    24241 acctggcggt ggaggagtgt atttttcgcc agatgcccgc cacgcagcgc gttctgtttc
    24301 tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta
    24361 atacccggcg gatggaagaa gataacgtcc gcctggcacg gcgcagtagc ggtggcggcg
    24421 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    24481 ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgcttggc gtcagcgccg
    24541 aagcgtccgg gcgtaacgat ctggtggtga aaaccgccga aggcgaccgc aaagtctcag
    24601 gatcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcaccttg ctgctcaatg
    24661 ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    24721 gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc
    24781 atgagcaggt ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag
    24841 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaatctttg
    24901 cccgtcagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    24961 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    25021 cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    25081 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    25141 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    25201 taaggtaact cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag
    25261 gagcgggaga ttatcaattt aatgttaagc cagcgtattt cc
//
LOCUS       DAJVTY010000044        25233 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVTY010000044 DAJVTY010000000
VERSION     DAJVTY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25233
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.044"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..394)
                     /locus_tag="OQQ66_001631"
     CDS             complement(<1..394)
                     /locus_tag="OQQ66_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1904603.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKN"
     gene            773..1084
                     /gene="rpsJ"
                     /locus_tag="OQQ66_001632"
     CDS             773..1084
                     /gene="rpsJ"
                     /locus_tag="OQQ66_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1904604.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1117..1746
                     /gene="rplC"
                     /locus_tag="OQQ66_001633"
     CDS             1117..1746
                     /gene="rplC"
                     /locus_tag="OQQ66_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1904605.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1757..2362
                     /gene="rplD"
                     /locus_tag="OQQ66_001634"
     CDS             1757..2362
                     /gene="rplD"
                     /locus_tag="OQQ66_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1904606.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2359..2661
                     /gene="rplW"
                     /locus_tag="OQQ66_001635"
     CDS             2359..2661
                     /gene="rplW"
                     /locus_tag="OQQ66_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1904607.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2679..3500
                     /gene="rplB"
                     /locus_tag="OQQ66_001636"
     CDS             2679..3500
                     /gene="rplB"
                     /locus_tag="OQQ66_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1904608.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3517..3795
                     /gene="rpsS"
                     /locus_tag="OQQ66_001637"
     CDS             3517..3795
                     /gene="rpsS"
                     /locus_tag="OQQ66_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1904609.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3810..4142
                     /gene="rplV"
                     /locus_tag="OQQ66_001638"
     CDS             3810..4142
                     /gene="rplV"
                     /locus_tag="OQQ66_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1904610.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4160..4861
                     /gene="rpsC"
                     /locus_tag="OQQ66_001639"
     CDS             4160..4861
                     /gene="rpsC"
                     /locus_tag="OQQ66_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1904611.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4874..5284
                     /gene="rplP"
                     /locus_tag="OQQ66_001640"
     CDS             4874..5284
                     /gene="rplP"
                     /locus_tag="OQQ66_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1904612.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5284..5475
                     /gene="rpmC"
                     /locus_tag="OQQ66_001641"
     CDS             5284..5475
                     /gene="rpmC"
                     /locus_tag="OQQ66_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1904613.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5475..5729
                     /gene="rpsQ"
                     /locus_tag="OQQ66_001642"
     CDS             5475..5729
                     /gene="rpsQ"
                     /locus_tag="OQQ66_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1904614.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5894..6265
                     /gene="rplN"
                     /locus_tag="OQQ66_001643"
     CDS             5894..6265
                     /gene="rplN"
                     /locus_tag="OQQ66_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1904615.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6276..6590
                     /gene="rplX"
                     /locus_tag="OQQ66_001644"
     CDS             6276..6590
                     /gene="rplX"
                     /locus_tag="OQQ66_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1904616.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6605..7144
                     /gene="rplE"
                     /locus_tag="OQQ66_001645"
     CDS             6605..7144
                     /gene="rplE"
                     /locus_tag="OQQ66_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1904617.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7159..7464
                     /gene="rpsN"
                     /locus_tag="OQQ66_001646"
     CDS             7159..7464
                     /gene="rpsN"
                     /locus_tag="OQQ66_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1904618.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7498..7890
                     /gene="rpsH"
                     /locus_tag="OQQ66_001647"
     CDS             7498..7890
                     /gene="rpsH"
                     /locus_tag="OQQ66_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1904619.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7903..8436
                     /gene="rplF"
                     /locus_tag="OQQ66_001648"
     CDS             7903..8436
                     /gene="rplF"
                     /locus_tag="OQQ66_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1904620.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8446..8799
                     /gene="rplR"
                     /locus_tag="OQQ66_001649"
     CDS             8446..8799
                     /gene="rplR"
                     /locus_tag="OQQ66_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1904621.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8814..9317
                     /gene="rpsE"
                     /locus_tag="OQQ66_001650"
     CDS             8814..9317
                     /gene="rpsE"
                     /locus_tag="OQQ66_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1904622.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9321..9500
                     /gene="rpmD"
                     /locus_tag="OQQ66_001651"
     CDS             9321..9500
                     /gene="rpmD"
                     /locus_tag="OQQ66_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1904623.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9504..9938
                     /gene="rplO"
                     /locus_tag="OQQ66_001652"
     CDS             9504..9938
                     /gene="rplO"
                     /locus_tag="OQQ66_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1904624.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9946..11277
                     /gene="secY"
                     /locus_tag="OQQ66_001653"
     CDS             9946..11277
                     /gene="secY"
                     /locus_tag="OQQ66_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1904625.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11309..11425
                     /gene="rpmJ"
                     /locus_tag="OQQ66_001654"
     CDS             11309..11425
                     /gene="rpmJ"
                     /locus_tag="OQQ66_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1904626.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11572..11928
                     /gene="rpsM"
                     /locus_tag="OQQ66_001655"
     CDS             11572..11928
                     /gene="rpsM"
                     /locus_tag="OQQ66_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1904627.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11945..12334
                     /gene="rpsK"
                     /locus_tag="OQQ66_001656"
     CDS             11945..12334
                     /gene="rpsK"
                     /locus_tag="OQQ66_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1904628.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12368..12988
                     /gene="rpsD"
                     /locus_tag="OQQ66_001657"
     CDS             12368..12988
                     /gene="rpsD"
                     /locus_tag="OQQ66_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1904629.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13014..14003
                     /gene="rpoA"
                     /locus_tag="OQQ66_001658"
     CDS             13014..14003
                     /gene="rpoA"
                     /locus_tag="OQQ66_001658"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1904630.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14044..14427
                     /gene="rplQ"
                     /locus_tag="OQQ66_001659"
     CDS             14044..14427
                     /gene="rplQ"
                     /locus_tag="OQQ66_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1904631.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14534..14902
                     /locus_tag="OQQ66_001660"
     CDS             14534..14902
                     /locus_tag="OQQ66_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS1904632.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14913..15338
                     /gene="zntR"
                     /locus_tag="OQQ66_001661"
     CDS             14913..15338
                     /gene="zntR"
                     /locus_tag="OQQ66_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS1904633.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15394..15612
                     /gene="arfA"
                     /locus_tag="OQQ66_001662"
     CDS             15394..15612
                     /gene="arfA"
                     /locus_tag="OQQ66_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS1904634.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15609..16019)
                     /gene="mscL"
                     /locus_tag="OQQ66_001663"
     CDS             complement(15609..16019)
                     /gene="mscL"
                     /locus_tag="OQQ66_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS1904635.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16149..17525)
                     /gene="trkA"
                     /locus_tag="OQQ66_001664"
     CDS             complement(16149..17525)
                     /gene="trkA"
                     /locus_tag="OQQ66_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1904636.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17547..18836)
                     /gene="rsmB"
                     /locus_tag="OQQ66_001665"
     CDS             complement(17547..18836)
                     /gene="rsmB"
                     /locus_tag="OQQ66_001665"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1904637.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18882..19829)
                     /gene="fmt"
                     /locus_tag="OQQ66_001666"
     CDS             complement(18882..19829)
                     /gene="fmt"
                     /locus_tag="OQQ66_001666"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1904638.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19844..20353)
                     /gene="def"
                     /locus_tag="OQQ66_001667"
     CDS             complement(19844..20353)
                     /gene="def"
                     /locus_tag="OQQ66_001667"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1904639.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20483..21607
                     /gene="dprA"
                     /locus_tag="OQQ66_001668"
     CDS             20483..21607
                     /gene="dprA"
                     /locus_tag="OQQ66_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1904640.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21579..22052
                     /locus_tag="OQQ66_001669"
     CDS             21579..22052
                     /locus_tag="OQQ66_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1904641.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22081..22623
                     /locus_tag="OQQ66_001670"
     CDS             22081..22623
                     /locus_tag="OQQ66_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS1904642.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22628..23200
                     /gene="tsaC"
                     /locus_tag="OQQ66_001671"
     CDS             22628..23200
                     /gene="tsaC"
                     /locus_tag="OQQ66_001671"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1904643.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23205..24023
                     /gene="aroE"
                     /locus_tag="OQQ66_001672"
     CDS             23205..24023
                     /gene="aroE"
                     /locus_tag="OQQ66_001672"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1904644.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24020..24277
                     /locus_tag="OQQ66_001673"
     CDS             24020..24277
                     /locus_tag="OQQ66_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1904645.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24253..24807)
                     /locus_tag="OQQ66_001674"
     CDS             complement(24253..24807)
                     /locus_tag="OQQ66_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1904646.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6455 a         5994 c         6564 g         6220 t
ORIGIN      
        1 ggttttttaa tgattctttt cctctcatga gcagaccaat aactacggct ccgcaagcga
       61 atgaggcagc aaggaaaacc agtcgtggta agaaggccca tgaatgccag gcgccaaggg
      121 cggcgagaaa cttcacatca ccatagccta atccttcttt atgtcgcagt atgcgatacc
      181 cccagtaaat gacagcgaat gtgccgtaac caacaattgt cccccataac gcatcggcta
      241 aaccatcagg atgacaaact tgatagaaca aaagacctga ccagagtaac gggcacgtaa
      301 atctgtcagg taacaagcca tgctttgcat cccagaaaaa aagtagggct gataaacaag
      361 cgtacaaaat cagaaaggga agggtagtgt ccataaaaca tcctctgtaa ttggagtatc
      421 accatcgaat aactccggat gctcagcaac agagagagcg ctgatttgtt aacctgattc
      481 cggtgaaaaa cgctatcttc acaaccttac aaatttcgtg gaatgcagct cgcaatctgc
      541 gcgttgcgat taatttctac ccgatgcttg cgtcaggctc agatttgcgt ataatgcgcg
      601 ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct
      661 aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat
      721 gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa
      781 ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc
      841 ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc
      901 gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga
      961 tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac
     1021 cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg
     1081 ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa
     1141 gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa
     1201 gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt
     1261 caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc
     1321 gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa
     1381 gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac
     1441 gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt
     1501 acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag
     1561 aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa
     1621 cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg
     1681 gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag
     1741 gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc
     1801 cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta
     1861 tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc
     1921 cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag
     1981 cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa
     2041 agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca
     2101 ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc
     2161 acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga
     2221 aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat
     2281 cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca
     2341 agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg
     2401 cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt actcaaagtt
     2461 gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa
     2521 gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc
     2581 ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac
     2641 ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac
     2701 cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca
     2761 aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc
     2821 gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca
     2881 aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt
     2941 ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta
     3001 aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta
     3061 acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac
     3121 caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg
     3181 atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact
     3241 gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag
     3301 caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg
     3361 tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt
     3421 ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg
     3481 tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt
     3541 ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag
     3601 aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc
     3661 atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt
     3721 cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa
     3781 gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc
     3841 gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc
     3901 aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc
     3961 tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag
     4021 ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag
     4081 gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct
     4141 gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat
     4201 tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga
     4261 cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg
     4321 tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg
     4381 tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat
     4441 cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa
     4501 actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat
     4561 gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag
     4621 cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc
     4681 gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg
     4741 tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt
     4801 tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata
     4861 aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg
     4921 taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt
     4981 tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc
     5041 agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa
     5101 gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat
     5161 tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc
     5221 attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat
     5281 gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg
     5341 aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag
     5401 tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag
     5461 aaggctggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca
     5521 aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta
     5581 aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg
     5641 gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg
     5701 ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg
     5761 ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc
     5821 gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta
     5881 gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc
     5941 acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg
     6001 cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga
     6061 tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt
     6121 cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg
     6181 tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat
     6241 ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg
     6301 acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc
     6361 tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg
     6421 ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca
     6481 acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag
     6541 acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag
     6601 tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga
     6661 gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg
     6721 tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc
     6781 aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat
     6841 ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt
     6901 ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc
     6961 taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc
     7021 agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac
     7081 tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa
     7141 gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta
     7201 gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg
     7261 aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat
     7321 tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg
     7381 cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg
     7441 ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg
     7501 agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac
     7561 aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag
     7621 gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt
     7681 actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca
     7741 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
     7801 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
     7861 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
     7921 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
     7981 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
     8041 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
     8101 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
     8161 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gtaatgtgat taacctgtct
     8221 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
     8281 actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg
     8341 gatctgcgcg cctaccgtcg tcctgagcct tataaaggca agggtgttcg ttacgccgac
     8401 gaagtcgtgc gtatcaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
     8461 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
     8521 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
     8581 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
     8641 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
     8701 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
     8761 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
     8821 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
     8881 aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta
     8941 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
     9001 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctgcaac
     9061 accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct tccgaaggta
     9121 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
     9181 ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg
     9241 gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
     9301 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
     9361 tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc
     9421 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
     9481 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
     9541 aaaaggcggg taaacgcttg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
     9601 gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
     9661 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
     9721 ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga
     9781 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
     9841 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
     9901 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
     9961 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
    10021 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
    10081 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac
    10141 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat
    10201 atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt ggcggaaatt
    10261 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgcta cggtactctg
    10321 gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg
    10381 caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc
    10441 acaggaacga tgttcctgat gtggttgggc gaacagatca ctgaacgagg tatcggcaac
    10501 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
    10561 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
    10621 ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg
    10681 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
    10741 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
    10801 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
    10861 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
    10921 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
    10981 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
    11041 atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac ctttatctgc
    11101 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
    11161 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
    11221 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
    11281 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg
    11341 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
    11401 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
    11461 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg
    11521 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
    11581 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
    11641 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
    11701 aagatcagtg agctgtctga aggacaaatc gatacgctgc gtgacgaagt tgccaaattt
    11761 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
    11821 ggttgctatc gcggcttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
    11881 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
    11941 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
    12001 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
    12061 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
    12121 tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat
    12181 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
    12241 tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa
    12301 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag
    12361 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
    12421 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
    12481 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
    12541 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
    12601 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
    12661 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
    12721 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
    12781 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
    12841 tctggagctg gctgagcagc gtgaaaagcc aacttggctg gaagttgatg ctggcaagat
    12901 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
    12961 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
    13021 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
    13081 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
    13141 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
    13201 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
    13261 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
    13321 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa
    13381 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
    13441 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
    13501 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
    13561 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca
    13621 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
    13681 ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga
    13741 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
    13801 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
    13861 aacgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
    13921 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac
    13981 cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag
    14041 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
    14101 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
    14161 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
    14221 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
    14281 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
    14341 tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca
    14401 gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg
    14461 gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc
    14521 ccctggagta tttatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca
    14581 agcgaaaggt gagtttgata atctggcagg tagcggcgaa ccattgatac tggatgatga
    14641 ttctcatgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ctggttgctt
    14701 accgccagaa cttgagcagc ggagggaagc aattcagctt ctggatatcc tgagaggtat
    14761 ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa
    14821 gctgcgacaa gctggattta gtaccgattt tttacgcggc gattatgctg acaagttgtt
    14881 ggacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa
    14941 tggcggaagt aacacctgac acgattcgtt attacgaaaa acagcagatg atggagcatg
    15001 aagtgcgtac tgaaggcggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat
    15061 ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga
    15121 tccgcatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat
    15181 tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc
    15241 ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgttcgatt cttgaagctc
    15301 ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg gcaggattac
    15361 actcgcgccg taaaataacc aattggagtt tttatgagcc gatatcagca tactaaaggg
    15421 cagataaggg ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgcgta
    15481 gagaaaaata agaaggggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg
    15541 aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggtct tctgctttca
    15601 ggcgcttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc tgccagtaat
    15661 acttcttctt tagttggtgc aggtgcggca gctggttctt cttttttccg attcagtttg
    15721 ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc aaagacgttt
    15781 tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccctgcgc atcgcgtagc
    15841 gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg
    15901 atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc
    15961 gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg
    16021 ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca ttttcctgcc
    16081 aggaatgaat ttaattaatc cttgataatc aaagggaaat aataaggcgt cattagacgc
    16141 cttattaatt acaagaagaa agggcttggc tggaagagtc tttcgacgtc ggtaataaac
    16201 tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg
    16261 gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg
    16321 atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct
    16381 tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc tgcttttcgc
    16441 acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaattgcaat atcgatcacg
    16501 ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca
    16561 cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca
    16621 ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca
    16681 ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta
    16741 cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata
    16801 ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc
    16861 atcaccgcgc ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttcaaca
    16921 atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta
    16981 tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag cgggccgcca
    17041 taataggctt taactacagc gaggctgact ttaccctcag cgaagttcac cacctgcaat
    17101 gcgcctggat actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc
    17161 agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg tacgtagtct
    17221 ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag
    17281 gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca
    17341 cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat
    17401 ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtaat atcgttgttc
    17461 tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt
    17521 ttcatcagtc tcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg
    17581 tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg
    17641 caaagttcgg catcagcggt acgttgcaaa aaagctttaa tctgcaggct attctcttcc
    17701 ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg
    17761 gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac
    17821 catttaatat ccggatggcg acgaatcacc cccgttgctg aacaaggcgc atctaataaa
    17881 atgcgatcaa actgttgctc gccacaccat tgggaagggt aacggccatc accttgtttc
    17941 acggttgcct tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg
    18001 tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta
    18061 ccgccggggg cggcacaaag atccagaatg tgttcaccgt tttgtggcgc aagccaggtc
    18121 atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc
    18181 gcatgaacag gtgcaggtgt ttccagacgt acagcatcag ggtaatccgc atgcgggaaa
    18241 cctttcattc ctgcttcatc cagcaatgca agccagctgt cgcgggaatg atgcgtacga
    18301 ttaacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc
    18361 tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca
    18421 ctggcattaa acttggctaa taactcttct tgctgacgct ggaactggcg taatacaccg
    18481 ttaatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac cgtttcagcc
    18541 agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg
    18601 taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag
    18661 tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct
    18721 ttatcagaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg
    18781 acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt
    18841 ataaaaatgc ccggtaagac cgggcttaga agagtggact atcaggccag acggttgccc
    18901 ggaacaaacc attcccgacg agaattcagg aggtcttgcg cgctcatcgc tttcttaccc
    18961 gcaggttgta acgagagcag attcaggatt ccatcaccag ttgcaacctg aatgccttgt
    19021 ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgacgct
    19081 ttccagactt taaccggctg tctttcaatt tccagccagc tcattggcca tggattgaaa
    19141 gcgcgaatgt agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct
    19201 ttactcaact tttcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg
    19261 ccgtctgcca gttgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg
    19321 tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt atagagcatg
    19381 tcaccggtgt ctaaaccgac atccatttgc ataatggtca caccagtttc tgcatcaccc
    19441 gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga
    19501 acgttgatac agccaagacg cggcatctcc agcactgctt taggcagaat taagccatag
    19561 gcgacgacga ccataacatc agcctgcaga tcggcgacca gttgctggtt ttcttgtgga
    19621 cgcagggaaa caggttgaaa aacgggcaga cctttttcct cagccagaac tttaaccggg
    19681 ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg
    19741 acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc
    19801 gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca
    19861 gacgatccag tttttcaact ttctgacgaa tacgttgttg tttcaacggt gacagataat
    19921 ccataaacag tttgccgacc aggtgatcca tctcatgctg aatacagatg gctaacagac
    19981 cgtctgcttc caattcaaat ggtttaccgt cgcggtccag ggcgcgaatt ttaactttct
    20041 ctgcgcgtgg cactaaagca cgttgttcag ggatcgacag gcaaccttct tcaatgcctg
    20101 tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt
    20161 ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc gccaggccaa
    20221 taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat
    20281 tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt
    20341 gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat
    20401 tctagacaaa tccccctctg attgacagca tcactgacca atcgcaaaga ttgctaaggc
    20461 tgcttatggc agggagataa ggatggtcga tacagatatt tggctgcgtt taatgagcat
    20521 cagcagcttg tacggcgatg atatggtccg tatagctcac tggctggcaa aacagtcgca
    20581 tattgatgcg gttgtattgc agcaaacagg gcttacattg cggcaggcac aacgctttct
    20641 ttcatttccg cgaaagagta tcgaaagctc actttgttgg ttggagcaac ccaaccatca
    20701 tttaatccct gcggacagcg aattttatcc tcctcaactt ctggcgacga cagattaccc
    20761 cggcgcactg tttgttgaag gagaactgta cgcgctgcat tcatttcagc ttgccgtagt
    20821 ggggagtcgg gcgcattcat ggtatggcga gcgatgggga cgattatttt gcgaaactct
    20881 ggccacgcgt ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg gtgtggcgca
    20941 taaagcggcc ttacaggtaa atggcgtcag cattgctgta ttggggaacg gacttaatac
    21001 cattcatccg cgccgccatg cccgactggc taccagtttg cttgaacatg gtggggcact
    21061 tgtctcggaa tttcccctcg atgtttcacc ccttgcttac aatttcccac gaagaaatcg
    21121 cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc gcagtggttc
    21181 gttggtgaca gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct tgccaggacc
    21241 aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg cgattcttgt
    21301 gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt tgccagacgc
    21361 ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat ttcctgagct
    21421 cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca
    21481 acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat ggatcgcagc
    21541 tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta ctaatgtatt
    21601 tgtttgaaac ctatattcac acagaagctg agttgcgtgt ggatcaagac aaacttgaac
    21661 aggatcttac cgacgcagga tttgatcgag aagatatcta caatgccctg ctttggctgg
    21721 aaaagcttgc ggattatcag gaaggactga cagaaccgat gcaactggcc tctgatccac
    21781 tctccatgcg tatttatacc cctgaggagt gtgaaagact ggatgccagc tgtcgtggtt
    21841 ttctgctttt ccttgagcaa attcaggtgc ttaaccttga aatccgtgaa atggtgatag
    21901 agcgagtgct cgcgctggat accgcagagt tcgacctgga agacctgaaa tgggtaatcc
    21961 tgatggtgtt gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg gaagaattac
    22021 tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc ataagttgtt
    22081 atggcgaaat cagcactgtt cacggtgcgt aataatgagt cctgcccaaa gtgcggggct
    22141 gaactggtta ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca gtatccggcg
    22201 tgtgactacg tccgtcctct gaaatcttca gcggatggac atattgtcaa agttctggag
    22261 gggcaggttt gccctgcatg tggcgcaaat ctggtattac gccagggacg ctttggtatg
    22321 tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga taaaccggac
    22381 gaaacagcaa ttacatgccc ccaatgtcgg acgggccatc tggtccagcg ccgctcccgt
    22441 tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc cattaacttc
    22501 aaacccatag ctggagaatg ccctgagtgt cattatccac tactcatcga aaagaaaacc
    22561 gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa
    22621 taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa
    22681 tgaagaacgt gtcatcgcct atccaacgga agccgttttc ggtgttgggt gcgatcctga
    22741 tagcgaaaca gcagtgatga gactgttgga gttaaaacag cgtccggttg ataaggggct
    22801 gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca ccatgctgac
    22861 tgatgcgcag cgtgaaacca ttttttcccg ctggccaggt cctgtcacct ttgtctttcc
    22921 cgcgcctgcg acaacaccgc gctggttgac gggccgcttt gattcgcttg ctgtacgagt
    22981 caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac
    23041 cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc gcgcacaatt
    23101 tggcgcggcg ttcccggttg tgcttggtga aacggggggg cgtttaaatc cttcagaaat
    23161 ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa acctatgctg
    23221 tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa tttgctcagc
    23281 aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat ttcatcaaca
    23341 cactgaacgc tttctttagt gctggtggta aaggtgcgaa tgtgacggta ccttttaaag
    23401 aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct ggtgctgtta
    23461 ataccctcaa gcggttagaa gatggacgct tgcagggtga caataccgat ggtataggtt
    23521 tgttaagcga tctggaacgt ctgtctttta tccgtccggg tttacgtatt ctgcttatcg
    23581 gcgctggtgg agcatctcgc ggcgtactac taccactcct ttccctggac tgtgcggtga
    23641 caatcactaa tcggacggta tcccgtgcgg aagagttggc taaattgttt gcgcacactg
    23701 gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat ctcattatta
    23761 atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg ctcattcatc
    23821 taggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt ctggcatggt
    23881 gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg gcacaggcgg
    23941 ctcatgcctt tcttctctgg cacggtgttc tgcctgacgt agaaccagtt ataaagcaat
    24001 tgcaggagga attgtccgcg tgaatcaggc catccagttt ccggacaggg aagagtggga
    24061 cgagaataaa aaatgtgtat gttttcccgc tctcgtgaat ggtatgcaac tgacatgcgc
    24121 gatctctggc gagagtctgg cgtatcgctt tactggagat acgccagaac agtggttagc
    24181 gagttttcgt cagcatcgct gggacctgga agaagaagcg gaaaacttaa ttcaggaaca
    24241 aagtgaagat gatcaaggct gggtctggtt accctgatcc agatattcgt ccttccattt
    24301 cacgtaatta ttcgcggaat agcgtaaccc agccttctct tcatcactta acgggcggat
    24361 ctgtttgacg gggctaccga gatacagata tccgctctcc agccgtttat tttgtgggac
    24421 cagactaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa gtaaaattga
    24481 gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca ccttgtgacc
    24541 aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg aggatttatg
    24601 agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct gtacataatg
    24661 tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca gacgaacgcc
    24721 accaatcacg acactgctat cgtcgatcat tacgcgctga ccgatttgtg gaaaaagatc
    24781 gcggtatggg cgtaaaacat cagacatact tacctcagca ataaatgatt tactaatgac
    24841 tttgggggca ttattggcct tgtgcaagtc ttttagtatg caaaaaagca ccgttttgtg
    24901 tgcgattgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca aaaaaatact
    24961 tgtgcaaaat attgggatcc ctataatgcg cctccgttaa gacgacaacg tgaaacactt
    25021 cacaggatgg tcggaacaac gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    25081 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagccgcgtc acaactgctc
    25141 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    25201 agacgaaaaa tgaataccaa gtctcaagag tga
//
LOCUS       DAJVTY010000045        25098 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVTY010000045 DAJVTY010000000
VERSION     DAJVTY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25098
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.045"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..383
                     /gene="hyfA"
                     /locus_tag="OQQ66_001675"
     CDS             <1..383
                     /gene="hyfA"
                     /locus_tag="OQQ66_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1904647.1"
                     /translation="SLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSLKDGE
                     ENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQRQMKE
                     KQRLAASWFANGGEDPLSLTQEQH"
     gene            383..2400
                     /gene="hyfB"
                     /locus_tag="OQQ66_001676"
                     /pseudo
     CDS             383..2400
                     /gene="hyfB"
                     /locus_tag="OQQ66_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            2411..3358
                     /gene="hyfC"
                     /locus_tag="OQQ66_001677"
     CDS             2411..3358
                     /gene="hyfC"
                     /locus_tag="OQQ66_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1904648.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            3375..3485
                     /locus_tag="OQQ66_001678"
     CDS             3375..3485
                     /locus_tag="OQQ66_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCS1904649.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            3497..4604
                     /locus_tag="OQQ66_001679"
                     /pseudo
     CDS             3497..4604
                     /locus_tag="OQQ66_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            4616..5266
                     /gene="hyfE"
                     /locus_tag="OQQ66_001680"
     CDS             4616..5266
                     /gene="hyfE"
                     /locus_tag="OQQ66_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1904650.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            5271..6851
                     /gene="hyfF"
                     /locus_tag="OQQ66_001681"
     CDS             5271..6851
                     /gene="hyfF"
                     /locus_tag="OQQ66_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1904651.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPK
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            6841..8556
                     /gene="hyfG"
                     /locus_tag="OQQ66_001682"
     CDS             6841..8556
                     /gene="hyfG"
                     /locus_tag="OQQ66_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCS1904652.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            8566..9105
                     /gene="hyfH"
                     /locus_tag="OQQ66_001683"
     CDS             8566..9105
                     /gene="hyfH"
                     /locus_tag="OQQ66_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1904653.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            9102..9860
                     /gene="hyfI"
                     /locus_tag="OQQ66_001684"
     CDS             9102..9860
                     /gene="hyfI"
                     /locus_tag="OQQ66_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1904654.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            9853..10266
                     /gene="hyfJ"
                     /locus_tag="OQQ66_001685"
     CDS             9853..10266
                     /gene="hyfJ"
                     /locus_tag="OQQ66_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1904655.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            10296..12308
                     /gene="hyfR"
                     /locus_tag="OQQ66_001686"
                     /pseudo
     CDS             10296..12308
                     /gene="hyfR"
                     /locus_tag="OQQ66_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            12330..13174
                     /locus_tag="OQQ66_001687"
                     /pseudo
     CDS             12330..13174
                     /locus_tag="OQQ66_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(13212..14273)
                     /locus_tag="OQQ66_001688"
     CDS             complement(13212..14273)
                     /locus_tag="OQQ66_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1904656.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            14486..15949
                     /gene="bepA"
                     /locus_tag="OQQ66_001689"
     CDS             14486..15949
                     /gene="bepA"
                     /locus_tag="OQQ66_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1904657.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            15970..16329
                     /gene="arsC"
                     /locus_tag="OQQ66_001690"
     CDS             15970..16329
                     /gene="arsC"
                     /locus_tag="OQQ66_001690"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1904658.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(16467..17213)
                     /gene="hda"
                     /locus_tag="OQQ66_001691"
     CDS             complement(16467..17213)
                     /gene="hda"
                     /locus_tag="OQQ66_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1904659.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(17263..18552)
                     /gene="uraA"
                     /locus_tag="OQQ66_001692"
     CDS             complement(17263..18552)
                     /gene="uraA"
                     /locus_tag="OQQ66_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1904660.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(18638..19264)
                     /gene="upp"
                     /locus_tag="OQQ66_001693"
     CDS             complement(18638..19264)
                     /gene="upp"
                     /locus_tag="OQQ66_001693"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1904661.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            19588..20625
                     /gene="purM"
                     /locus_tag="OQQ66_001694"
     CDS             19588..20625
                     /gene="purM"
                     /locus_tag="OQQ66_001694"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1904662.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            20625..21263
                     /gene="purN"
                     /locus_tag="OQQ66_001695"
     CDS             20625..21263
                     /gene="purN"
                     /locus_tag="OQQ66_001695"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1904663.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            21435..23501
                     /gene="ppk1"
                     /locus_tag="OQQ66_001696"
     CDS             21435..23501
                     /gene="ppk1"
                     /locus_tag="OQQ66_001696"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1904664.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            23506..25047
                     /gene="ppx"
                     /locus_tag="OQQ66_001697"
     CDS             23506..25047
                     /gene="ppx"
                     /locus_tag="OQQ66_001697"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1904665.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         5712 a         6571 c         6679 g         6136 t
ORIGIN      
        1 aaagcctctg tattggctgc aagctttgcg ccgtggtctg cccatttggc gcaatcagcg
       61 cttcaggaag ccgtccggtg aatgcccctg cgcaatatgt ttttcaggct gacggctcac
      121 tcaaagatgg cgaagaaaac gcgccgaccc aacatgcttt gctgcgctgg gaaccgggtg
      181 tccagaccgt cgcggtgaaa tgcgacctgt gtaatttctt gccagaaggt ccggcctgcg
      241 ttcgcgcttg cccgaatcag gcgttacggc tgattaccga cgatagcctg caacgtcaga
      301 tgaaagaaaa acagcgcctt gccgcaagct ggtttgccaa tggcggggag gatccccttt
      361 ccctcactca ggagcaacac taatggatgc cctgcaatta ttaacctggt cgctgatcct
      421 ctttctgttt gccagtctgg cttcgctgtt tttactcggt ctggacagac tggctattaa
      481 gctttccggc atcacatcgc tggtgggcgg cgtgattggc atcatcagcg gaattacgca
      541 attacatgct ggtgtaactt tagtcgcccg ttttgccacc ccttttgact ttgccgattt
      601 aaccctgcga atggatagcc tctcggtatt tatggtgctg gttatctcct tgctggtggt
      661 ggtttgttcg ctctattcat tgacttatat gcgcgaatac gagggcaaag gcgcggcggc
      721 gatgggcttc tttatgaatc ttttcatcgc atcgatggtt gccctgctgg tgatggacaa
      781 cgctttttgg ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt ttctggtcat
      841 tgccaggcag gataaaacgt cgatcaacgc tggcatgctc tactttttta tcgcccacgc
      901 cggatcggtg ctgattatga tcgccttctt gctgatgggg cgcgaaagcg gcagcctcga
      961 ttttgccagt ttcgcacgct ttcactttct ccggggctgg cgtcggcgat gtttctgctg
     1021 gcctttttcg gttttggcgc gaaagccggg atgatgccgt tgcacagctg gttgccgcgc
     1081 gctcaccctg ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat ggtcaaaatc
     1141 ggtattttcg gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg tttgcctctg
     1201 tggtggggca ttctggtgat ggcgatcggc gcaatctccg cgctcctggg cgtgctatat
     1261 gcgctggcgg aacaggatat caaacggctg ctggcctgga gtaccgtcga aaacgtcggc
     1321 attattttgc tggcagtcgg tgtggcgatg gacggtctgt cactgcacga cccgctgctc
     1381 accgtggttg gactgctcgg cgcactgttt catctgctca accatgcgct gttcaaaggg
     1441 ctgctatttc tcggcgcggg cgcgattatt tcgcgtttgc atacccacga catggaaaaa
     1501 atgggggcac tggcgaaacg gatgccgtgg acagccgcag catgcctgat tggttgcctc
     1561 gcgatatcag ccattcctcc gctgaatggt tttatcagcg aatggtacac ctggcagtcg
     1621 ctgttctcac taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc tattgctatg
     1681 gtaatgctgg cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat gtacggtatt
     1741 actttctgtg gtgcgccgcg cagtacacac gctgaagagg cacaggaagt gccaaatacg
     1801 atgatcgtcg ccatgctact gctcgcggca ctctgcgtat taattgcgct tagtgccagt
     1861 tggctggcac cgaagataat gcatattgcc catgcgttta ccaatacccc tcccgtcact
     1921 gtcgccagcg gaatagcact tgtacccggc acgtttcata cacgggtcac tccttcatta
     1981 ctgttgctgt tactactggc gatgcctttg ctgcctggcc tttactggct gtggtgtcgt
     2041 tcgcgccgcg cagcgtttcg tcgcacagga gatgcctggg catgcggcta cggctgggaa
     2101 aacgcgatgg ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt cttctctgcg
     2161 ttatttcgtc tacgacaaca gctcgaccct acgctgaggc taaataaaga tcttgcgcac
     2221 gtcaccgcca gggctcagag cacagaaccc ttctgggatg agcgggtgat ccgccccatc
     2281 gtgagcgcca cccaacggct ggccaaagaa atacagcatc tgcaaagcgg cgactttcgt
     2341 ctctattgcc tgtatctagt tgccgcactg gttgtgctgc taatcgctat tgccgtctaa
     2401 ggaaatcacc atgagacaaa ctctttgcga cggatatctg gtcatttttg cgttagcaca
     2461 ggccgtgatt ctgctgctgc taaccccgct ttttacgggt atttcccggc agatacgcgc
     2521 gcgtatgcac tcccgccgcg ggccggggat ctggcaggat tatcgcgaga tccacaaact
     2581 gtttaaacgc caggaagttg cgcctatatc ttcaggtctg atgttccgcc tgatgccgtg
     2641 ggtattaatc agcagcatgc tggtgctggc gatggcccta ccacttttta ttaccgtttc
     2701 cccttttgcg ggcggcggcg atctgatcac ccttatctat cttcttgccc tgtttcgttt
     2761 tttctttgct ctttccggac tggataccgg aagcccgttt gcgggagtcg gtgccagtcg
     2821 cgagttgacg ctcggcattc tggtcgaacc catgctcatt ctctcactgc tggtattggc
     2881 gctgatagca ggttccacgc atatcgagat gatcagcaat acgctggcaa cgggctggaa
     2941 ctcgccgcta accaccgtac tggcgttact ggcctgtggt tttgcctgct tcattgagat
     3001 gggaaaaatt ccctttgatg ttgctgaagc agaacaggaa ttacaggaag gcccgctgac
     3061 cgaatattcc ggtgccgggc tggcgctagc gaaatggggg ctggggctga aacaggtcgt
     3121 gatggcagca ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag aactttctct
     3181 cgcctgcctg ctgacttcac ttgtcgttac gctgctcaag gttttgctga tttttgtact
     3241 ggcctcaatc gcagaaaaca cgctggcacg cgggcgtttt ttactcattc accatgtgac
     3301 ctggcttggc ttcagccttg ctgcgcttgc atgggtcttc tggttaaccg gtctgtaagg
     3361 agcactgacg gaatatggaa aatcttgctc tgacgacgtt aatgctgcct tttatcggcg
     3421 cactggtcgt ttcgttttcg ccacaacgtc gggccgccga atgggggttt tgttcgccgc
     3481 actgaccacg ctgtgcatgt tgtcgctgat ctccgcgttt tatcaggccg ataaagttgc
     3541 cgtcacgttg acgttggtca acgtggggga tgtggcattg tttggcctgg tcattgatcg
     3601 cgtgagtacg ctgattctgt ttgtggtggt gtttctcggt ttgctggtca cgatctactc
     3661 cacgggttat ctgacggata aaaatcgcga acacccgcat aacggcacga atcgttatta
     3721 cgcatttttg ctggtgttta tcggcgcgat ggcgggactg gtactctcct cgacgctgct
     3781 cggtcagttg ttgttttttg aaattagggc ggctgctcct gggcgttgat cagttattac
     3841 cagagcgata aagcgcagcg ttcaacacta aaagcgttgc ttatcaccca tatcggttcg
     3901 ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt tgtgcttagc
     3961 gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat cctgtttgcc
     4021 gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc aatggaagcg
     4081 ccaacaccga tcagagccta tctccacgcc gcatcgatgg tgaaagtggg cggctggcac
     4141 tggagggcag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg tttttccttg
     4201 tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg cgtggcgtat
     4261 tgcactccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggcg attaccggtg
     4321 tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt tttgcgttgt
     4381 cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa tcggtcgcca
     4441 gtttcgcctg gtttattcgc tggtttggtc gcgttgtgcc tggcaaaccg agcgaggccg
     4501 tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta ctgattgtga
     4561 tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat gatgaatgat
     4621 cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc tgtttgtgat
     4681 tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc tggtgctggt
     4741 gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga tctggtccgc
     4801 cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg ctgcacgaaa
     4861 tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg cactgctcgc
     4921 ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac cgatggctac
     4981 cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc tgctgtgcat
     5041 tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg aaaacggctc
     5101 ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa taggtatcgc
     5161 taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa tatggcgtac
     5221 ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag atgagttatt
     5281 ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc tgttttgcct
     5341 gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc ttagggatca
     5401 cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca ggagaaatat
     5461 tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc gccattcttg
     5521 gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat gaagtggcac
     5581 acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat ctgtttttgt
     5641 tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg gcgatcgaag
     5701 ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca tcgctggaat
     5761 cagcatggaa gtacatcatt atttgtactg ttgatgtcgc ttttggtctg ttcggtaccg
     5821 tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg atattctgga
     5881 gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg gcctttgtgt
     5941 ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc tggctgccgg
     6001 atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta ttgctgaact
     6061 gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaagccatc ggcagcgatt
     6121 tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg gcatttttca
     6181 ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag aacatggggc
     6241 tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg ctgctgcaca
     6301 ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat gtactgctca
     6361 agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg ccatttaccg
     6421 ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc aacatttttc
     6481 ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg attatcgtcc
     6541 tgctgttatt gctgttaacg ctggtgctgg cgggcctggt acggatggct gcgcgggtgt
     6601 taatggcgaa accgccgaag gccgttaacc ggggtgatct cggctggttg accacctcgc
     6661 caatggtgat tctgctggtc atgatgctgg cgatgggaac gcatattcca caacctgtca
     6721 tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac ctgcctgcac
     6781 aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg gagaaacaca
     6841 gtgaacgtta attcatcgtc aaatagtggc gaagcgattc tcgccgccct gaaaacgcag
     6901 ttccccggcg cggtactgga tgaagagcga caaacgcctg aacaggtcac cattacggtg
     6961 aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga tggctggctt
     7021 ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt ttattatgcc
     7081 ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt cgatgccgac
     7141 agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg cgagcgagaa
     7201 attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg cctggtgttg
     7261 cccgatgact ggccggaaga tatgcatccg ctgcacaaag atgcgatgga ttatcgactg
     7321 cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg caacagcgat
     7381 gcgcgggtga tccctgtcgg cccgctgcat atcacctccg atgaaccggg tcacttccgc
     7441 ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta tgtccatcga
     7501 ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt cttatcggac
     7561 cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc ggttgaaaat
     7621 gcactgggga ttgaggtgcc gcaacgagcg cataccattc gctcgattct gctggaagtc
     7681 gaacggctac acagtcattt gctcaacctt ggcctctcct gccattttgt tggttttgat
     7741 accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc ggaattgctg
     7801 accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg cgatattctc
     7861 aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga cgtgtcggag
     7921 ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg cattggcatt
     7981 ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg cggcagtggt
     8041 tttgcccgtg atttgcgctt tgatcacccc tacgccgact acggcaatat tccaaaaaca
     8101 ctgtttacct tcaccggcgg cgatgttttc tcccgcgtga tggtccgtgt caaagagacg
     8161 tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca tgccggatac cccactgctg
     8221 accgaaggct ttagctataa acctcacgca ttcgcgctgg gctttgttga agcgccacgc
     8281 ggtgaagacg tgcactggag catgctcggt gataaccaaa aattgttccg ctggcgctgc
     8341 cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg caataccgtt
     8401 tctgacgcac cgctgattat cggtagcctt gatccctgct actcctgtac cgaccgtgtg
     8461 acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc cgtataaaga gatcgaacgc
     8521 tacggcattg atcgtaaccg ttcgccgctg aagtaaggac agaagatgct gaagttactg
     8581 aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc actggaggtc
     8641 agcccaggct ttcgcgggaa accggacctg atgcccagcc aatgtattgc ctgcggcgcc
     8701 tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg accagcaaaa ttcgcgcacc
     8761 tggcagctct atctggggcg ttgtatttac tgcggacgtt gtgaagaagt gtgcccgacc
     8821 agagccatcc agcttaccaa taactttgaa ctgaccgtca ccaataaagc cgatctctat
     8881 acccgcgcga cgttccatct acaacgttgc agccgttgcg aacgcccgtt tgccccgcaa
     8941 aaaaccgtcg cactggctgc tgaattgttg gcacagcaac aaaatgcgcc acaaaaccgc
     9001 gaaatgttgc gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc gacgctgatc
     9061 aacgacgata cagatgtacc gctggtggct aaggagcagc tatgagtcca gtgcttacac
     9121 aacatgtcag ccagcccatc acgctggatg agcaaacgca aaagatgaag cggcatttgc
     9181 tacaggatat ccgtcgctca gcctacgttt atcgcgtcga ttgcggcggc tgcaacgcct
     9241 gtgaaatcga aatttttgct gccatcaccc ccgtattcga cgcagaacgt tttggcatta
     9301 aggttgtttc atcaccgcgt cacgccgata ttttgttatt tactggcgca gtcacccggg
     9361 cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc cgatcataaa atctgtgttt
     9421 cctacggcgc gtgcggtgtc ggcggcggta ttttccacga tctctacagc gtctggggcg
     9481 gcagcgacac cattgtcccc attgatgttt ggatccccgg ctgcccgcca acaccggccg
     9541 ccaccattca cggtttcgcc gtggcgctcg gtttgctgca acagaagatt cacgctgtgg
     9601 attatcgcga tcccaccggg gtgactatgc aaccgttgtg gccacagatc ccgccatcac
     9661 agcgtatcgc cattgagcga gaagcgcggc ggctggcggg ctatcgtcag gggcgagaaa
     9721 tttgcgatcg gctcctgcgc catttaagcg acgatcctac aggaaatcgg gttaacacct
     9781 ggttgcgcga tgccgacgat ccacgtctca atagtatcgt tcagcaactc tttcgcgtac
     9841 tccgggggtt acatgactga agagtgcggg gaaattgttt tctggacgct gcgaaaaaag
     9901 tttgtcgcca gtagcgacga gatgccggaa caccgctctc aggtaatgta ttactcgctg
     9961 gctatcggcc atcacgttgg cgtgattgat tgtctgaatg tcgccttccg ctgcccactg
    10021 acggaaaacg aagattggct tgcacaggtc gaagaggagc aagcccgacg taagatgctg
    10081 ggggtgatga cctttggtga gattgttatt gacgccagcc acaccgccct gttgacccgg
    10141 gcattcgcgc tactggcaga tgacgcgacg tctgtgtggc aggcgcgtag cattcaattc
    10201 attcatctgt tggatgaaat tgtgcaggaa ccggccatct atctgatggc cagaaaaatt
    10261 gcgtgagaag gatttctcat taataaggac tgttgatggc tatgtcagac gaggcgatgt
    10321 ttgccccgcc acaaggaata acaattgaag cggtaaacgg aatgctcgcg gagcggttag
    10381 cacagaaaca cggtaaggcg tctttattac gcgccttcat cccgatgccg ccgccgttca
    10441 gcccggtaca acttattgaa ctgcatgtta tcaaaagcaa cttctattac cgctaccatg
    10501 atgatggcag cgatgtgacg gcaacaacag agtatcaggg cgagatggtc gattattcgc
    10561 gtcacgccgt ccttctcggc agtagtggaa tggcggagct acgctttatt cgtacccacg
    10621 gcagccgttt tactccctag gattgcacac tgtttaactg gctggcacgc attatcactc
    10681 cggttctgca atcatggctc aatgatgaag aacaacaggt ggcgctgcgt ttgctggaga
    10741 aagatcgcga tcatcatcgg gtactggttg atattactaa tgcagtgctg tcacatcttg
    10801 atctcgacga tctgatcgct gacgtcgctc gtgagatcca tcattttttc ggtctggctt
    10861 cagtcagtat ggtactgggc gatcatcgaa agaacgagaa gtttagcctg tggtgcagcg
    10921 atctttctgc ctcacattgt gcgtgtctgc cacgcaatat gcctggcgac agtgtattgc
    10981 tgacacaaac gctacaaacc cgacaaccga ccttgacgca ccgtgcagac gatctgtttc
    11041 tctggcaacg cgacccgtta ttactcttac ttgcatctaa cggctgcgaa tctgcgctcc
    11101 ttataccgct tacctttggc aaccatacac cgggtgcatt gttgctggcg catacctctt
    11161 ccactctctt tagtgaggaa aactgccagc tactacaaca catagccgat cgcatcgcta
    11221 ttgccgttgg caatgccgat gcctggcgta gcatgaccga tttgcaggaa agtttgcagc
    11281 aagaaaacta ccagcttagc gagcagctcc tttcgaatct gggcatcggt gacattatct
    11341 atcaaagcca ggcaatggaa gacctgctcc agcaggtaga tattgtagcg aagagcgaca
    11401 gtacggtgtt gatttgtggt gaaaccggaa ccggcaaaga ggtgatcgcc agagcgctcc
    11461 atcaacttag cacgcgacgc gacaaaccgc tggtcaaaat caactgcgct gccatccccg
    11521 ccagtcttct ggaaagtgag ttattcggtc atgacaaagg ggcgtttact ggtgcgatta
    11581 atacacatcg tggtcgtttt gaaattgccg atggcggcac gttgtttctc gatgaaattg
    11641 gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt attgcaggaa cgggagattg
    11701 agcgtctcgg cgggagtaga acgatcccgg tgaatgtcag agtcattgcc gccaccaacc
    11761 gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag caatctcttt tatcgcctga
    11821 atgtcttccc attggaattg ccgccgctac gcgaccgtcc ggaagatatc cctcttttag
    11881 caaaacattt cacgcaaaaa atggcgcgcc atatgaatcg cgcaattgac gccatcccga
    11941 ccgaggcgct acgccagttg atgtcgtggg actggccggg caacgtgcac gagctggaaa
    12001 acgtgattga gcgagcggta ctgctgactc gcggtaacag tctgaattta catcttaatg
    12061 tccgacaaag ccgtttactg ccgacgctaa atgaagattc agcgcttcgc agttcaatgg
    12121 cgcagttgct gcacccgacg acgccagaga atgacgaaga agaacgtcag cgcattgttc
    12181 aggtattgcg agaaaccaat ggcattgttg ccgggccccg tggcgcggcg acacgattag
    12241 ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct ggggatctcg gttcgcgagg
    12301 tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa actctctttc gacttgcagt
    12361 tgagcgccag aaaagcggca atcgctgaac ggattgccgt ccataaaatt gcccgcagta
    12421 aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatc ggatttactt
    12481 tttacctttc cgttatcgcc gatgccccgt cttcacaggc attaacccat ctggtgggcg
    12541 gcctttgctt tacactcggc tttattttgc tggcggtttg cggcacagcc tgttcacctc
    12601 gtcggtaatg acggtgatag caaaaagtcg gggcgttatt agttggcgaa cttggctgat
    12661 taacgcactt ctggtggcct gtggtaatct ggcaggtatt gcctgtttca gtttgttaat
    12721 ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcgg ttttacactg
    12781 cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca ttatgtgcaa
    12841 tctgatggtt tgcctggcgc tgtggatgag ttattgcggg cgttcgttat gcgacaaaat
    12901 cgtcgccatg attttgccca tcaccctgtt tgtcgccagt ggctttgagc actgtatcgc
    12961 caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgcccccc ccttctggca
    13021 actggcgcac agtagcgcag acaattttcc ggcactgacg gtcagccatt ttattaccgc
    13081 caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg tgagtatgtg
    13141 ttatcgggct atttatttac gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt
    13201 cgggctgaag attattcttg cgcgatttgc ccatcgggcc aggcgtggat cacggctttg
    13261 atcagcgtcg ccaatggaat ggcgaagaat acgccccaga atccccacaa accaccgaag
    13321 atcaccaccg ataaaataat caccagcgga tgcaggttaa ccgcttcgga gaacaacacc
    13381 ggtactagca ggttgccgtc cagcgcctga ataatcagat acaccgcgaa gcagctccag
    13441 aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat ggtcaccaca
    13501 aatgcgccaa tgtacggaat aagaaccgag aagccgacca gcaccgccag cagcagcgaa
    13561 tagttcagcc caaagagcaa gaaccccagc caggtggcga tccccaccac gatcatctcc
    13621 agcactttgc cgcggatata gttggtgatt tgttgattca tctccttcca cacctgtcct
    13681 gccagtccac ggttgcgcgg cagcacccga cgaacggcgt tcagcatctg ctctttgtct
    13741 ttcagcagga agaagaccat caatggcacc agcaccagat agacggcaat ggtcagcaaa
    13801 ccgaccagcg aggcgaggga aattttcacc accgaatcgc ccatggtcag catccgactg
    13861 cgcatatttt cggccattgc atcaataatg cccgcgtcca ttaacgccgg atagcggcgc
    13921 ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc ccggcatatc gcggattaag
    13981 tagatgccct gttgccaggc gataggcagt accacgaacg ccatcagtag caatataccg
    14041 acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg agcagccaat agattgcagg
    14101 cgcacggttg gccattccag caaataggcc agcacaatag ccaccagcaa cggagcaagc
    14161 aggccactaa agaaaaagat aatgccaaat ccggcaacta aaataaccag caaggcaatc
    14221 gcttccgggt cgctaaaacg gcggcgatac cattgcatca acatttcgag cataaaaacc
    14281 ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt cacaaaaaag
    14341 acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg ctaatcttcg
    14401 ccgttacact caaaggcggc gcggtgggaa cgatatttca cagtatcggt caaatgacta
    14461 tcttcagaaa tacaggatag aggttatgtt caggcagttg aaaaaaaacc tggttgcaac
    14521 cctcattgct gctatgacca ttggtcaggt agccccggcg tttgccgaca acgcagacac
    14581 cttgccggat atgggaacct ccgcaggaag cacgctttcc attggtcagg aaatgcagat
    14641 gggcgactat tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg acccgctgtt
    14701 aacgcaatat attaattcgc tggggatgcg tctggtttcg catgccaatt cggttaagac
    14761 accgtttcat ttttttctaa tcaacaacga cgaaattaac gcctttgctt tctttggcgg
    14821 caacgtggtg ctgcactctg ccctgttccg ttattccgat aacgaaagtc aactggcttc
    14881 agttatggcg cacgaaatct cccacctcac ccaacgtcac ctggcgcgag cgatggaaga
    14941 tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta ggttctattt tactggcgat
    15001 ggccagtccg caggcgggga tggcggcgct gaccggtaca ctggcgggaa cgcgtcaggg
    15061 gatgatcagt ttcacccagc aaaatgaaca ggaagcggac cgcattggta ttcaggtgct
    15121 gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc ctcgaaaaat tactcgatca
    15181 ggcgcgttac tcctcgcgcc cgccggaaat tttattgact cacccgttgc cggaaagtcg
    15241 tctggcagat gcccgcaacc gtgctaatca gatgcgaccg atggtggtgc agtcgtcgga
    15301 agatttctat ctggcgaaag cgcgcacact ggggatgtat aattccggac gtaaccagct
    15361 caccagtgat ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac gagcggcgca
    15421 atatggtcgt gctttacagg cgatggaagc caataaatac gacgaggcgc gtaaaacgct
    15481 gcaaccgtta ctggcggcag aacctggcaa cgcatggtat ctcgatctgg ctaccgatat
    15541 cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg cccgtgattt
    15601 gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct tatctgcaag gcggtcaacc
    15661 acaagaagcg gccaatattc tcaatcgcta cacctttaat aataaagatg acagcaacgg
    15721 ctgggattta ctggcacagg cggaagccgc gctaaataac cgcgatcagg agctggctgc
    15781 gcgagcagaa ggttatgcgc tcgccggacg actcgatcag gccatttcgc tgttgagtag
    15841 cgccagttcg caggtgaaat taggcagcct gcaacaagcg cgttacgatg cgcgcatcga
    15901 ccagttgcgc cagctgcagg aacgctttaa gccttatacc aagatgtaat tcgcattaaa
    15961 ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc cacgctgttc aaagagccgg
    16021 gaaacgctga acctgctgaa agaaaacggc gtggagccag aagtggtact ctaccttgag
    16081 acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat gaatagcgcc
    16141 cgtgaattga tgcgccagaa agaggatctt tataaagaac tgaacctggc tgacagttcg
    16201 cttagcgaag aggcgctgat tcaggcaatg gtggataatc cgaagctgat ggagcgcccg
    16261 attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt actggaaatc
    16321 gtgggttaag atgtgctcca tcgctagttg aagcacattg ccggatgcga cgcttgccat
    16381 gtcttatccg gcctacgaat actatggatt aacttcgcgt agttcggata aggcgttcgc
    16441 gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac aaacggaatg
    16501 gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt cataaatagc
    16561 gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc cggcagttca
    16621 aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc agaaagtggc
    16681 tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga gatccggtaa tcccagattc
    16741 aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga ttccagaatt
    16801 cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat acactcaatg
    16861 ttgtcgatac aaaccagcga caaatgctcc ataccgtcga gcacttccgg aacaaaccaa
    16921 gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa ttccgcgcaa
    16981 gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata gatgtaaccg
    17041 ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt atcccccggc
    17101 cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag ctgtgccggt
    17161 gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac cacagaatcc
    17221 catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt ctgttatatc
    17281 cgcctcttct gcgtccagca ccacttcttc agggcgcagc acactgatca gtttgaaaat
    17341 caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact ccgccgcgcc
    17401 gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca ggatcaggtt
    17461 ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg aagcaccgat
    17521 gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca tctggatagc
    17581 ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc cgccgataac
    17641 ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt aagtggtatt
    17701 tggcgtggag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa acatcgaacg
    17761 gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta ctaccaggtg
    17821 ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa tggcaaacca
    17881 ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag tgagcattaa taatcggcgt
    17941 ggtatcgaca attcccattg cgaaagagag cgcgtagccc accagcacgc caattaaaat
    18001 cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga ccgccagggt
    18061 ggtaatagag atgatgatgg ttttggagtc tggcgtttgc ccttcagccg ggagtaaacc
    18121 cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga ttgcgcccat
    18181 tgccgcaggt ggaaacagca cgtccagcca gccggtcccc gctttcttca cgataaaaga
    18241 aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt cataccctaa
    18301 cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat aagccggaat
    18361 tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata acagtacagt
    18421 cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca tggcgaacaa
    18481 atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca ccccgatagc
    18541 acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac tcaaaaaaaa
    18601 gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca aagattttgt
    18661 caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc tgatcaatcg
    18721 atgcggtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata ccttctggcg
    18781 cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg
    18841 tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc atacgctcat
    18901 cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct tcattacggt
    18961 acataccgac aacgctgatg cgcgcgctcg gaacattttc cagcacaccg tccatcatac
    19021 caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc tggtcgattt
    19081 ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt
    19141 cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg gtgctgatat
    19201 cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact tccacgatct
    19261 tcatactctt tctcctttga ggggcagcca caaaaaaatc gacggattat acctcctttc
    19321 ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa tgataacgat ttgagataaa
    19381 gttattttat attcaaatgg ttatgaaaga agatcattcc atccgaaaac taacctttgc
    19441 cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag acgtgcaaaa aggttgtgca
    19501 aagcagcctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt atttttctac
    19561 cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag ctacaaagat
    19621 gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg cgtagtgaag
    19681 aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcgct gtgtgcattg
    19741 ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg taccaagctg
    19801 cgtctggcaa tggacttaaa acgtcacgac accattggta ttgatctggt cgccatgtgc
    19861 gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt tcctcgacta ttacgcaacc
    19921 ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg gcatcgcgga aggttgtctg
    19981 caatcgggct gttcactggt gggtggcgaa acggcagaaa tgccggggat gtatcacggt
    20041 gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga aatcatcgac
    20101 ggctctaaag tcagcgacgg cgatgttctg attgcactcg gttccagcgg tccgcactcg
    20161 aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc gcaaaccacc
    20221 gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat ttacgtgaag
    20281 tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct gaccggcggc
    20341 ggcttctggg aaaacattcc acgcgtattg ccagataata ctcaggcagt gattgatgaa
    20401 tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa cgttgagcgc
    20461 catgaaatgt atcgcacctt caactgcggc gtcgggatga ttattgccct gcctgctccg
    20521 gaagtggaca aagccctcgc cctgctcaat gccaacggtg aaaacgcgtg gaaaatcggt
    20581 atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat attgtggtgc
    20641 ttatttccgg taacggaagt aatttacagg caattattga cgcctgtaaa accaacaaaa
    20701 ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc cttgaacgcg
    20761 cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac agtcgtgaag
    20821 cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg gtcgtgctgg
    20881 ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg cgtttgctga
    20941 acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt caggcgctgg
    21001 aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa ctggacggtg
    21061 gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa gatgacgtca
    21121 ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc tggtttgccg
    21181 atggtcgtct gaaaatacac gaaaacgccg cgtggctgga tggtcaacgt ctgccgccgc
    21241 agggctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct gccgctggcg
    21301 tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt ttctggcata
    21361 gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt gaataaaacg
    21421 gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc agttggttat
    21481 cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg attgaaagga
    21541 tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc cgcttcgctg
    21601 aactgaagcg acgcatcatt attagcgaag aacaaggctc caactctcat tcccgccatt
    21661 tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac ggcctctaca
    21721 acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa cgccagctct
    21781 ccgtcaatca acaaaactgg cttcgtcatt attttaagca gtatctgcgt cagcacatta
    21841 cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaggat gattacacct
    21901 atctggcggt ggaaattatc cgtggagaca ccatccgtta cgcgctgctg gagatcccat
    21961 cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga cgcaagccga
    22021 tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa ggcttctttg
    22081 attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa tacgatttag
    22141 tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag cagcgtttaa
    22201 ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg gttgaagtgt
    22261 tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt cgttatcata
    22321 attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg aacaaaccac
    22381 tgccgcgttt acgccacatt tggtttgata aagcccagtt ccgcaatggt tttgatgcca
    22441 ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat gtgctggaac
    22501 tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac atttaccgcg
    22561 tggcgaaaga ttcacgcatc atcgactcga tgatccacgc cgcacataac ggtaagaaag
    22621 tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt cactgggcga
    22681 agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg aaaattcacg
    22741 ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac gcacacatcg
    22801 ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg ttgctgaccg
    22861 cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa aacccatacc
    22921 gcccagtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt ctgttatatg
    22981 aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt atcaccctga
    23041 agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc tccagctccg
    23101 gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat ccggaaggca
    23161 ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat gaccgggttt
    23221 atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac tggatgacgc
    23281 gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcac ctgaagcagc
    23341 gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt tatatcgata
    23401 aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg gcgcagttgg
    23461 cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc aatacacgat
    23521 aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag ttttcacatg
    23581 gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa acagcgagtg
    23641 catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat gacgcgcggt
    23701 ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc cagcgtctgt
    23761 atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct gaaacgcgtg
    23821 gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc ccgtctgatt
    23881 tttatgggcg tggaacatac ccaaccggag aaaggtcgca aactggttat tgatattggc
    23941 ggcggatcta cggaactggt gattggtgaa aatttcgaac cgattctcgt tgaaagccgc
    24001 cggatgggtt gtgtcagctt tgcccagctt tacttccctg gcggggtcat caataaagag
    24061 aattttcagc gcgctcgtat ggcggcagca caaaaactgg aaactttaac ctggcaattc
    24121 cgtattcagg gctggaacgt ggcaatgggc gcttccggta ccataaaagc cgcccatgaa
    24181 gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct ggaaaaactg
    24241 gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga gtttaccggg tctttccgaa
    24301 gagcggaaaa cagtcttcgt tcctggactg gcgattttat gcggtgtgtt tgatgcttta
    24361 gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact gtacgaaatg
    24421 gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct cgccaaccag
    24481 tatcacatcg acagcgaaca ggcccgacgg gtgctggata ccactatgca aatgtacgaa
    24541 cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct actgcgatgg
    24601 gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca tcgccactcc
    24661 gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca gctgatgatg
    24721 gcgacactgg tgcgctatca ccgcaaagcg attaaactcg acgatctgcc gcgctttacc
    24781 ttgtttaaga agaaacagtt cctgccactg attcagctat tgcgccttgg cgtattactc
    24841 aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac tgatgacagt
    24901 cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgttggt actgcttgat
    24961 ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa aattgaagaa
    25021 gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc gacgagaaat gtcggcccgc
    25081 attattcagg cactttcg
//
LOCUS       DAJVTY010000046        25005 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVTY010000046 DAJVTY010000000
VERSION     DAJVTY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25005
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.046"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..808
                     /locus_tag="OQQ66_001698"
     CDS             <1..808
                     /locus_tag="OQQ66_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCS1904666.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OQQ66_001699"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OQQ66_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1904667.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="OQQ66_001700"
     CDS             1520..1798
                     /locus_tag="OQQ66_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904668.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="OQQ66_001701"
     CDS             complement(1845..3941)
                     /locus_tag="OQQ66_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1904669.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="OQQ66_001702"
                     /pseudo
     CDS             complement(3941..4678)
                     /locus_tag="OQQ66_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4675..5313)
                     /locus_tag="OQQ66_001703"
     CDS             complement(4675..5313)
                     /locus_tag="OQQ66_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1904670.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKILLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="OQQ66_001704"
     CDS             complement(5427..5669)
                     /locus_tag="OQQ66_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904671.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OQQ66_001705"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OQQ66_001705"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1904672.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="OQQ66_001706"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="OQQ66_001706"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1904673.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="OQQ66_001707"
     CDS             9906..10178
                     /locus_tag="OQQ66_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1904674.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OQQ66_001708"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OQQ66_001708"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1904675.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12478
                     /locus_tag="OQQ66_001709"
     CDS             11531..12478
                     /locus_tag="OQQ66_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1904676.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            12548..13354
                     /locus_tag="OQQ66_001710"
     CDS             12548..13354
                     /locus_tag="OQQ66_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1904677.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEDHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            13364..13771
                     /locus_tag="OQQ66_001711"
     CDS             13364..13771
                     /locus_tag="OQQ66_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1904678.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13771..14265
                     /locus_tag="OQQ66_001712"
     CDS             13771..14265
                     /locus_tag="OQQ66_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1904679.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14331..15128
                     /locus_tag="OQQ66_001713"
     CDS             14331..15128
                     /locus_tag="OQQ66_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1904680.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15139..15981
                     /locus_tag="OQQ66_001714"
     CDS             15139..15981
                     /locus_tag="OQQ66_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1904681.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16015..17244
                     /gene="sorE"
                     /locus_tag="OQQ66_001715"
     CDS             16015..17244
                     /gene="sorE"
                     /locus_tag="OQQ66_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1904682.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17627..18316
                     /gene="pepE"
                     /locus_tag="OQQ66_001716"
     CDS             17627..18316
                     /gene="pepE"
                     /locus_tag="OQQ66_001716"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1904683.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18407..20038)
                     /locus_tag="OQQ66_001717"
     CDS             complement(18407..20038)
                     /locus_tag="OQQ66_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1904684.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20258..23941)
                     /gene="metH"
                     /locus_tag="OQQ66_001718"
     CDS             complement(20258..23941)
                     /gene="metH"
                     /locus_tag="OQQ66_001718"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1904685.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEAQDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTDNAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNCIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            24141..24965
                     /gene="iclR"
                     /locus_tag="OQQ66_001719"
     CDS             24141..24965
                     /gene="iclR"
                     /locus_tag="OQQ66_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1904686.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5879 a         6613 c         6534 g         5979 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcagccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac cagggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aaatcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tctgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaaacagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt
    11821 acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg
    11881 gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt
    11941 aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa
    12001 cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc
    12061 ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac
    12121 catctctgcc tactgggata atctggatgt cgccctggtg ggaattggct caccggccat
    12181 tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc
    12241 ccgccaggtt gctggcgata tttgctcgcg cttttttgat attcacggcg caatggttga
    12301 aacgaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata
    12361 ttccattggc attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg
    12421 aaaatatatt aattgtctgg taacgaatag cagcacagct gaactattac tgaaataaaa
    12481 atcttcccga tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga
    12541 gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc
    12601 atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat
    12661 ggtagatatt cacggtggcg atggtcaata tgaagatcat aaaggctatc agttttggcc
    12721 gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt
    12781 tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga
    12841 tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa
    12901 tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca
    12961 acatgatggc gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca
    13021 aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga
    13081 attgggtaaa cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg
    13141 actgcgcacg ccggaatatg aagaagcgct ggtgtggacg cgcaatatca ccgtcgagca
    13201 gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga
    13261 agtcgctgat tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac
    13321 cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca atgccatttt
    13381 ttgcgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga
    13441 cgccagggtc gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa
    13501 gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca
    13561 gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt
    13621 aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa
    13681 tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa
    13741 acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac
    13801 cgcctgatcc acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt
    13861 attatctgca atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct
    13921 gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcac
    13981 aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta
    14041 gcgatggttc gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg
    14101 cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt
    14161 catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca
    14221 atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg
    14281 tacatatgct ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa
    14341 gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc
    14401 tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg
    14461 gtgatttaaa aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga
    14521 tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc
    14581 tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg
    14641 cggcaggcca ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg
    14701 cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt
    14761 ttggcgtgca ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg
    14821 ccgatatggt gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa
    14881 ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga
    14941 aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc
    15001 tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac
    15061 agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg
    15121 acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg
    15181 tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt
    15241 ttttgctacg acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt
    15301 gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc
    15361 atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt
    15421 accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg
    15481 gtctggggaa cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc
    15541 aacattctcg gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag
    15601 tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg
    15661 aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg
    15721 ctggtcacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc
    15781 gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg
    15841 ctggcgctcg gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta
    15901 tggctgattt tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc
    15961 agattcttcg ccccggcatg actgccgggg ccatcgctca acatgaggtg gtttatgaaa
    16021 acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct
    16081 gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc
    16141 tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac
    16201 cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag
    16261 cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc
    16321 ccggactgcc ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtagttatt
    16381 cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa
    16441 ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt
    16501 caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc
    16561 ggcggcaccg gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac
    16621 ccgtcgcagc tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac
    16681 tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat
    16741 acgctaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat
    16801 gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc
    16861 gccggaccgc aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca
    16921 tttacccact acgtgggcac gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa
    16981 ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat
    17041 gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat
    17101 accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg
    17161 attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat
    17221 gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg
    17281 ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag
    17341 aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
    17401 tttatgccgg atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata
    17461 ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
    17521 agtctcagct cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
    17581 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
    17641 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
    17701 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
    17761 ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
    17821 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt
    17881 cggcggcggg aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc
    17941 aattactgac gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct
    18001 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
    18061 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
    18121 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
    18181 gacgattatt ggtctaccgg aaggtaactg gatcacagtg agtaaaggtc acgctacgct
    18241 gggtggcccg aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc
    18301 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
    18361 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
    18421 gtccttcatc ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt
    18481 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
    18541 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
    18601 agcgatgctt actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
    18661 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
    18721 catcaagcga aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc
    18781 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc
    18841 ggcgcgactc ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg
    18901 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
    18961 caccgtgcat cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca
    19021 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca
    19081 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
    19141 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
    19201 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
    19261 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
    19321 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
    19381 aaccgaggtt agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg
    19441 cggtcagagc cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
    19501 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
    19561 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
    19621 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
    19681 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
    19741 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
    19801 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
    19861 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
    19921 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
    19981 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
    20041 gttcaggttc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
    20101 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
    20161 ccagcctgat tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
    20221 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    20281 tcggtgccag ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt
    20341 caacctgatc gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat
    20401 accaacccga aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg
    20461 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    20521 ccgggtagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    20581 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tggagatact
    20641 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    20701 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    20761 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacaaagtca gcgagacagt
    20821 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat
    20881 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    20941 ggcccaccac gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt
    21001 tggcgtcttt aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg
    21061 gatacttccc ggccagcgac caggtcataa agaacggtgt ccagtcgatg caattacgca
    21121 gagtttcgat gctggcttca acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt
    21181 aagcctgcca gtcaaaagcg aagtcgttat cgcgcgccgc ttccagcgtg accggtggtg
    21241 tgcgcggttt cttgcgcccg tgctgaatac gtacggtttc gtactccttg cgggtacgag
    21301 cgacaaaatc atcgcgctga gtatcggaaa gcagcgccgc caccacacca acggtgcgcg
    21361 aggcattctg cacatacacc gtcgggccgc tgtagttctg ctcaattttc accgccgtgt
    21421 gcgcttttga ggtggtcgcg ccaccgatca gtaacggaat ggtgaagccc tgacgctcca
    21481 tctctttcgc cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaaggccaa
    21541 tcaaatcagc attcacttct ttagcagtac ggagaatttt ttccgcaggc accatcacgc
    21601 cgagatcgac aatttcgtag ttgttacatt gcagcaccac gccaacgata tttttaccga
    21661 tatcgtggac gtcgcccttc acggtggcga tcaccatctt gccgttggtt ttgccctgct
    21721 ctttgctggc ttcaataaac ggttcgagat acgccaccgc ctgtttcatg acgcgcgccg
    21781 atttgaccac ctgtggcagg aacattttgc cttcgccaaa cagatcgccg accacgttca
    21841 tgccgtccat caacgggcct tcaatcactt caatcgggcg cgtagcctgc tggcgggctt
    21901 cttcggtatc ctgctcgata aactcggtaa tgcctttgac cagcgagtat tccagacgtt
    21961 tattcacttc ccacgagcgc cactccgcct gctgggcgtt atcggtgtcg tcggttttgc
    22021 tgccgcgata tttctcggca agctccagta aacgctcagt gccatcgtca cgacgattaa
    22081 gaatcacatc ttccaccgcg tcgcgcagtt cagcgggtag gtcgtcgtaa atcgccagtt
    22141 gccccgcgtt gacgatcccc atatccatgc cattgcgaat agcgtagtag aggaacactg
    22201 cgtgaatggc ttcgcgcacc ggatcgttgc cacggaatga gaaagaaacg ttagatacgc
    22261 cgccggaaat cagcgcgtgc ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt
    22321 cctgcgcgta gttgttgtgc tcttcaatac cggtggcgac cgcgaagatg ttcgggtcga
    22381 agatgatatc ttctggcggg aagccaacct cttcggtgag gattttgtac gcccgacggc
    22441 aaatctcgat tttccgtgcg cgagtatcgg cctgtccctg ttcatcaaag gccatcacca
    22501 ccactgccgc accgtagcga cgcaacaatt tcgcgtgatg gataaaggcg tcgacgccct
    22561 ctttcatcga gattgagtta acaatgcctt tgccctgaat acacttcagc cctttttcga
    22621 taacgtccca ttttgaggag tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg
    22681 caatcagatt gagaaaacgc accatcgccg cttcggcatc gagcatccct tcatccatgt
    22741 tgatatcgat aatctgcgcg ccgttttcca cctgctgacg cgccacatcc tgcgcctcgc
    22801 tgtatttctc ttctttgatc aggcgcttga acttagcaga gccggtgacg ttggtgcgtt
    22861 cacccacgtt cacaaacagg ctatcgtcgc caatgttcag cggctccagg ccagacaaac
    22921 ggcaggctac gggaatttcc ggcagtttgc gcggcgctaa tccttctact gcacgactca
    22981 tcgctgcaat atgttgcggc gtggtcccgc agcaaccacc gacgatattg agaaagcccg
    23041 cttgcgccca ttcacgtatc tgttttgcca tcgtgtcggc gtcgagatcg tattcaccaa
    23101 aggcgttagg cagcccggcg ttcgggtggg cggtgacgta gcattccgca atccgtgata
    23161 gttcctgcac gtactggcgc agttcatcgg gccccagcgc acagttcagg ccaaaggtta
    23221 gcgcttcggc gtggcgcaat gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc
    23281 gcccggaggc gtcggtgatg gtgccggaga tcataatcgg cagctcaacg cccagcgctt
    23341 caaactccgt tttcaccgca aaaaccgccg ctttggcgtt aagggtgtcg aaaacggttt
    23401 caatcaggat cagatccgca ccaccttcca ccagcgcttt ggtggattcg caataggccg
    23461 ccaccagctg gtcaaaagtg atgttgcgga atgccggatc gttgacgtcc ggagaaatag
    23521 acgccgtgcg gttggttggg ccgagaacac cggcaacgta gcgcggtttc tccggcgtgc
    23581 gcgcggtcca ctcgtcagca caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg
    23641 ccgacaggga ttccatctgg taatccgcca tcgcaatggt cgtggagttg aaggtgttgg
    23701 tttcgatgat atccgcgccc gcttcaaagt aggcgttgtg gatagcggcg atcacttccg
    23761 gtttgctgag caccagcagg tcgttgttgc ctttgagatc gcatggccag tcggcaaagc
    23821 gttcaccacg aaaatcggct tcgttcagtc gataactctg gatcatggtg cccataccgc
    23881 cgtccagcac cagaatacgt tcatttaact gcgcacgcag ttgttccact ttgctgctca
    23941 cacttgctcc cgacacgctc aacccagatt taataaaaat tcaacaaacc atactggcat
    24001 aaacgcatct gtggtaaaag cgaccaccac gcaacatgag atttgttcaa cattaactca
    24061 tcggatcagt tcagcaacta ttgcattagc taacaataaa aatgaaaatg attcccacga
    24121 tgcagaaaaa ggagactgtc atggtcgcac ccattcccgc gaaacgcggc agaaaacccg
    24181 ccgttgccac cgcaccagcg actggacagg ttcagtcttt aacgcgtggc ctgaaattac
    24241 tggagtggat tgccgaatcc aatggcagtg tggcactcac ggaactggcg caacaagccg
    24301 ggttacccaa ttccacgacc caccgcctgc taaccacaat gcaacagcag ggtttcgtgc
    24361 gtcaggttgg cgaactggga cactgggcaa tcggcgcaca tgcctttatg gtcggcagca
    24421 gctttctcca gagtcgtaat ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg
    24481 aagagtctgg cgaaacggtc aatatggagg tgcttgatca aagcgatcac gaagcgatta
    24541 ttatcgacca ggtacagtgt acgcatctga tgcgaatgtc cgcgcctatc ggcggtaaat
    24601 tgccgatgca cgcttccggt gcaggtaaag cctttttagc ccaactgagc gaagaacagg
    24661 tgacgaagct gctgcaccgc aaagggttac atgcctatac ccacgcaacg ctggtgtctc
    24721 ctgtgcattt aaaagaagat ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg
    24781 aggaacatgc gctggggcta cgttgccttg cagcgtgtat tttcgatgag caccgtgaac
    24841 cgtttgccgc aatttctatt tccggaccga tttcacgtat taccgatgac cgcgtgaccg
    24901 agtttggcgc aatggtgatt aaagcggcga aggaagtgac actggcgtac ggtggaatgc
    24961 gctgactttt tctggcgggc agaggccata ttctgcccat catac
//
LOCUS       DAJVTY010000047        24909 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVTY010000047 DAJVTY010000000
VERSION     DAJVTY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24909
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.047"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(33..371)
                     /locus_tag="OQQ66_001720"
                     /pseudo
     CDS             complement(33..371)
                     /locus_tag="OQQ66_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(487..1038)
                     /gene="maa"
                     /locus_tag="OQQ66_001721"
     CDS             complement(487..1038)
                     /gene="maa"
                     /locus_tag="OQQ66_001721"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1904687.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1210..1428)
                     /gene="hha"
                     /locus_tag="OQQ66_001722"
     CDS             complement(1210..1428)
                     /gene="hha"
                     /locus_tag="OQQ66_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1904688.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1454..1828)
                     /gene="tomB"
                     /locus_tag="OQQ66_001723"
     CDS             complement(1454..1828)
                     /gene="tomB"
                     /locus_tag="OQQ66_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1904689.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2374..5523)
                     /locus_tag="OQQ66_001724"
     CDS             complement(2374..5523)
                     /locus_tag="OQQ66_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1904690.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5546..6739)
                     /gene="acrA"
                     /locus_tag="OQQ66_001725"
     CDS             complement(5546..6739)
                     /gene="acrA"
                     /locus_tag="OQQ66_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1904691.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6881..7528
                     /gene="acrR"
                     /locus_tag="OQQ66_001726"
     CDS             6881..7528
                     /gene="acrR"
                     /locus_tag="OQQ66_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1904692.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7656..11018
                     /gene="mscK"
                     /locus_tag="OQQ66_001727"
     CDS             7656..11018
                     /gene="mscK"
                     /locus_tag="OQQ66_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1904693.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11230..11391)
                     /gene="rsmS"
                     /locus_tag="OQQ66_001728"
     CDS             complement(11230..11391)
                     /gene="rsmS"
                     /locus_tag="OQQ66_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1904694.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11405..11932)
                     /gene="priC"
                     /locus_tag="OQQ66_001729"
     CDS             complement(11405..11932)
                     /gene="priC"
                     /locus_tag="OQQ66_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1904695.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12002..12379
                     /locus_tag="OQQ66_001730"
     CDS             12002..12379
                     /locus_tag="OQQ66_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1904696.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12532..13083
                     /gene="apt"
                     /locus_tag="OQQ66_001731"
     CDS             12532..13083
                     /gene="apt"
                     /locus_tag="OQQ66_001731"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1904697.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13212..15083
                     /gene="dnaX"
                     /locus_tag="OQQ66_001732"
     CDS             13212..15083
                     /gene="dnaX"
                     /locus_tag="OQQ66_001732"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1904698.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14476..14540
                     /gene="dnaX"
                     /locus_tag="OQQ66_001732"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15136..15465
                     /locus_tag="OQQ66_001733"
     CDS             15136..15465
                     /locus_tag="OQQ66_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1904699.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15465..16070
                     /gene="recR"
                     /locus_tag="OQQ66_001734"
     CDS             15465..16070
                     /gene="recR"
                     /locus_tag="OQQ66_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1904700.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16180..18054
                     /gene="htpG"
                     /locus_tag="OQQ66_001735"
     CDS             16180..18054
                     /gene="htpG"
                     /locus_tag="OQQ66_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1904701.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18235..18879
                     /gene="adk"
                     /locus_tag="OQQ66_001736"
     CDS             18235..18879
                     /gene="adk"
                     /locus_tag="OQQ66_001736"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1904702.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            19011..19973
                     /gene="hemH"
                     /locus_tag="OQQ66_001737"
     CDS             19011..19973
                     /gene="hemH"
                     /locus_tag="OQQ66_001737"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1904703.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTCEL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19970..20929)
                     /gene="aes"
                     /locus_tag="OQQ66_001738"
     CDS             complement(19970..20929)
                     /gene="aes"
                     /locus_tag="OQQ66_001738"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1904704.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21081..22385
                     /gene="gsk"
                     /locus_tag="OQQ66_001739"
     CDS             21081..22385
                     /gene="gsk"
                     /locus_tag="OQQ66_001739"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1904705.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22518..24194)
                     /locus_tag="OQQ66_001740"
     CDS             complement(22518..24194)
                     /locus_tag="OQQ66_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1904706.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24432..>24909)
                     /gene="fsr"
                     /locus_tag="OQQ66_001741"
     CDS             complement(24432..>24909)
                     /gene="fsr"
                     /locus_tag="OQQ66_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1904707.1"
                     /translation="YYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGR
                     KYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGM
                     VSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6222 a         6490 c         6397 g         5800 t
ORIGIN      
        1 atggtagtgt ccgctattgc cagtacacct cacagcgcgg caggtacaaa gcgcgtgttg
       61 taccattcgc cgttatagca aacgcggaaa tccagcacgt cgatatcttc atagcggtag
      121 cgtgggtaga catcgaaaaa gaagaaacca ttaagcagga taaataatac aaccagtagc
      181 cagacagagc ctgacagcgt ttcggactgc aacatcaccg ccagggtggc aaaaaaagca
      241 acgtacatac cgataaacag ccccggatgt ttacggataa aactgatact aaagcggggt
      301 ttgttgtcgc gtttttcgcg ggtattcaga gactcaatcg tttcggtcag caggcgttgt
      361 atttcggtca tcgtttgcct tcgtggtatc tgcaactttc cagggaacac cctattttaa
      421 cgtgggaata gtagagggga agtaacagat ctaccacaat tttgcataac agttgcgaaa
      481 aaccgattac aattttttaa ttattctggc tggattaccg cccacgacaa cgttgtccgg
      541 gacacctttt gtgacaactg cacctgaagc taccacgacg ttatcaccaa tggtcacacc
      601 agggttaatg accgcgcgtc cgccaatcca gacgttatta ccgatggtga cgggtttccc
      661 cagttcagca ccgctattac gtgctacagg gtcgatggga tgtgttgccg tgtagatatg
      721 aacgcctggt gccaacatac agttatcacc gatgcgaata gggcagacat caagcatcac
      781 gcaatcgaag ttggcgaaaa aattattgcc gagaaaaatg ttatagccat aatcacagcg
      841 aaacgttggc tcaatataag cctctgtcac ctgaccgaat aaatcagcga gaatttgctg
      901 gcgtaatgtg tgctcttccg ccagggaatg attgtatcgg tgaataagct gacgagcgcg
      961 caggcgatcg cgagataacg tctcatctgc cgagcgatac aactcaccag caatcatctt
     1021 ttctttttct gtgctcatta ctgacctccg gttgctggaa atgcggcaac acagtaagac
     1081 gaaaagtcag tgaacagaac gttcccatta catctgtgtg ataaagatca catagggtat
     1141 gtcttcatgg tgaaaaagta taaaattctt aataaacagc cggttatagc tccgaaagcg
     1201 aattatttat tagcgaataa atttccatac tgaggaaggg atcttgtcgt atagtttatt
     1261 catggtcaat tcggcgaggc ggtgatctgc ggctgagtaa aataccgcca gttcattatc
     1321 tgataattcg tatttatttt tctcgataac acgctccagc gtgtcaattg tctggcaacg
     1381 acgtaaacgc attaaataat cggttttcgt taaaggcttt tcggacatac ttctacccat
     1441 ggttgtaata attctaacaa gataaactcg caggattctc tttcgtcgca ttgacaaaac
     1501 aacggaatag tcgattacct gacttccgcc atttctgaag atcctgcata ttaataccat
     1561 aactactgaa caacataaag gtgtcatcca gatattcgtc gatctgctca atgagcttat
     1621 tgtcttcatt atacttaatt ttgtaattaa gtgcgaaggt cgcaatatgc tcaatcagtt
     1681 cattcaactg gaggttgatc gccgaggttg ggtcgttaac ccagccataa ttgctttctt
     1741 caaggtttgc aaggcagtca tgatacaggg tttcacagag aaacttaagc tgtgcgatat
     1801 catgtctttt gggtgagtat tcatccataa cgcgtcccct tcttagcggt tgaactaacg
     1861 gacacctttc gggatggaaa aaacttactg acctggactt gccctctcgt ttgttagctt
     1921 aactataagc cactctttgc aggtattcat tgcattatta cgaaaaatta caattaggaa
     1981 ggaatcggga caggactcac tttttcatac tagcactaac ccgcagcagg taaaagcagt
     2041 ttgctcgaac tgttcataac atttcaggat tatcgccaga tgaaattggt gacactaata
     2101 ccaggattgc tctgaatatg acgtaataac cgaggaatga ataaagaatt accgcaaata
     2161 attaagaata gcctctaaat gattatggat tattaaatct attagcgcta cttaatataa
     2221 attaactaac aatcagtatt ttttatgaat tttatccgtg gttaatactg gttttcgtat
     2281 gagatcctga gttggtggtt caattactcc ttaatgttcg taggttatgc ataaaaaagg
     2341 ccgcttgcgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg
     2401 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
     2461 gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc
     2521 gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
     2581 cgccagcgag gtcatcagga tcggacgtaa acgcatccgc actgcatcaa gcgtcgcttc
     2641 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
     2701 gttcttcgcc gacaacccaa tggttgtgag caggcctact tggaagtaaa cgtcattggt
     2761 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
     2821 aacggagaac ggaatcgacc agctctcgta cagcgccgct agacacagga atacgacaat
     2881 caacgaaatc gcgtacagtg aaggtgcctg gttgccggag aggcgttcct gataggacat
     2941 ccccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccattagctc
     3001 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tggatggcag
     3061 gccattgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaatgccga
     3121 gaacggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
     3181 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
     3241 gtcgttcaca tagctaccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga
     3301 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     3361 cagaccgttt ggacgtacgc tggtcaacat atcaggatgc tttgctgctt ctgcaagcag
     3421 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     3481 gtcaaagccg gttgcagtac ccagttccac gattgcgggt aggttaaagg cgaaaaccat
     3541 cgcatctttg atttgcgaga aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt
     3601 ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac cggtattctg
     3661 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     3721 tttggtcaga tagtaatgcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc
     3781 acctgctggc agctgaacca tggtcataaa tacgccctgg tcctcatctg gcaagaagga
     3841 gcttggcaga cgcacgaaca gataggccat gccgaccacg atgatcagat acagcaccag
     3901 gtaacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     3961 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     4021 ggcaatcggt ttcagcatgg tggcacaaag agccggagtc aggatcaacg ctaccagtac
     4081 cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga
     4141 accgccaaag aaggccatcg gtacgaatac cgctgacagt accatcgcga taccgaccag
     4201 agcgccctga atctgcccca tcgacttacg ggtagcttct tttggcggca aaccttcttc
     4261 cgccataaca cgctccacgt tttctaccac aacgatagcg tcatccacca acaagccgat
     4321 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     4381 ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     4441 gttctgcagg aacagataca taaccaggaa cacgaggatg atcgcttcga ccagcgtttt
     4501 aaccacttcg tgaatagaga ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt
     4561 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     4621 atccagcgca tttgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt
     4681 aaactctgcg atgatgtcgt agttctcacc acccagctca atcttcgcca catcacgcag
     4741 caggacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga
     4801 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt
     4861 accaccgagc tgacccgccg caacctgggc gttctgcgct ttgatggcgg taatgacatc
     4921 aaccggcgtt agctggaatt tgttcagctc attcgggttc atccagatac gcatcgcgta
     4981 ctgtgaaccg aacaactgaa catcacccac gcccgacgta cggctgatgg catctttcat
     5041 attcgccgct acgtagtcag agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     5101 gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgaac
     5161 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     5221 atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtccccg tggagtcact
     5281 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     5341 caccgtgtcc tgcactgttt tcgcatcagc gccggggtag gaggcggaga tcgttactgc
     5401 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     5461 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     5521 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     5581 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     5641 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     5701 acttatcgcc aatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     5761 caactaccag tacggtggca tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta
     5821 aaatagcgtt tggattaagc ccttcttcca gacgtgcgcg cacgaacata cccggcagca
     5881 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa
     5941 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     6001 tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt
     6061 tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct
     6121 gcacggttgc cagcgcagtt gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg
     6181 acttaccaat gcgaccgcta atcggagcgg tgactttggt gtaagccaga ttgatccgcg
     6241 cagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca
     6301 gagcctgatc gtactcttgc ttactgatgt actgagtacc gagcagtttc tgataacgat
     6361 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac
     6421 tgtcgtatgt cgcctgatag gtcgcaggat caatctgata gagagagaca ccagcttcga
     6481 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     6541 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     6601 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     6661 tgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     6721 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     6781 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     6841 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     6901 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     6961 gggggtatca tccacctcgc tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc
     7021 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga
     7081 atccaatatt ggtgaactag agcttgagta tcaggcaaaa ttccctggcg atccactctc
     7141 agtattaaga gagatattaa ttcatgttct tgaatccacg gtgacagaag aacgacgtcg
     7201 attattgatg gagattatat tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca
     7261 acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca
     7321 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgggcag caattattat
     7381 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     7441 taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac
     7501 gcttcgtaat cctgccacta acgaataacc ctgaatctga ctccaggatt tttcctggac
     7561 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     7621 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     7681 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     7741 acacggcgtt tgcgcgggcg tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg
     7801 cgcaacttga ctcactaaat aaacaaaaag atctttctgc tcaggacaaa ctggagctgc
     7861 aggatctgac agatacatta gccaccctcg ataaaatcga tcgcataaaa gaagagacag
     7921 ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     7981 taacagcact tagcgatgtc gataacgacg aagaaacgcg caaaattctg agtacgcttt
     8041 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     8101 acgatctggc gtcttataac agccagctgg tttcgttaca gacgcagccc gaacgcgtgc
     8161 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     8221 ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag gctcagcagg
     8281 cgttgctgaa tgcggagatt gaccagcagc gtaaaagcct ggaagggaac accgtcttgc
     8341 aggatacctt gcaaaagcaa cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc
     8401 agttgcagct gttgcaagaa gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg
     8461 cgcaggaagc cgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaaac
     8521 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact gaaaacggta
     8581 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgggcg ctgcaatcgg
     8641 aacgcaatat taaagagcag attgccgtcc tgaagggcag cctgctgttg tctcgtatcc
     8701 tttaccagca acaacaaacg ctgccctcgg cggatgaact ggaaaacatg accaaccgca
     8761 tcgcggattt gcgtctcgaa cagtttgaag ttaaccagca gcgtgatgca ctcttccaga
     8821 gcgatgcgtt cgtcaacaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     8881 acgatgcgtt attgcaagtg gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac
     8941 agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag cagttaatga
     9001 gcgtatcgaa aaacctgaaa tccatcctga ctcagcaaat cttttgggtg aacagtaacc
     9061 gtccaatgga ctgggactgg atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt
     9121 cgatgaaaat cacggtgaac tgggaaaaag cctggcctgc cgtttttatc gctttcctcg
     9181 ccggtttgcc gctgctgttg attgtcgggc tgatccactg gcgtctgggc tggctgaaag
     9241 cgtatcaaca aaaactggct tccgctgtgg gttccctgcg taacgatagc cagctcaaca
     9301 caccaaaagc gatccttatc gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg
     9361 cggttggctt gattctgttg accatgcagc tcaacatcag cgaactgcta tggtcgttca
     9421 gcaaaaaact ggcgattttc tggctggtgt ttggcctgtg ctggaaggta ctggagaaga
     9481 acggcgttgc cgtgcgtcac ttcggcatgc cggaacagca gaccagccac tggcgtcggc
     9541 aaattgtccg catcagtctc gcattgctgc ctatccattt ctggtctgtg gtggcagaac
     9601 tttccccgct gcatctgatg gatgatgtgc tggggcaagc gatgattttc ttcaacctgc
     9661 tgctgattgc cttcctggta tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc
     9721 acaccatgcg actggtcacc attaccgtgc tgtcgattat cccgattgca ttgatggtgc
     9781 tgactgccac gggttatttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     9841 tttatctggt gatcatctgg aacttgctct accagacggt attgcgcggc ttaagcgtag
     9901 cggcgcggcg tatcgcctgg cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg
     9961 gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc actggaacag gttaaccagc
    10021 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
    10081 tttggtccga tttgatcacc gtgttcagct atatcgacag cattacgctc tggcattaca
    10141 acggtactga agctggcgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg
    10201 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag
    10261 tgctggtgct ctcgcgactg aatatgcgcc agggcgcgtc gtatgccatt actaccatcc
    10321 ttaactacat cattattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt
    10381 gggataaact ccagtggctg gcggcagcat tatccgtagg tcttggtttt ggtttacaag
    10441 aaattttcgg taacttcgtc tccggtttga tcattctgtt cgaacgtccg gtgcgtattg
    10501 gcgatacggt aaccattggt agcttctcgg ggacggtaag taagatccgt attcgtgcga
    10561 caacgattac cgatttcgat cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg
    10621 agcgtctgat caactggtcg ttgactgaca ctactacgcg tctggtgatc cgtctcggcg
    10681 tggcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc
    10741 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcgttt ggtgccagca
    10801 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg
    10861 atgagctgaa ccgtactatc gatcagctgt gccgtgaaaa cgacatcaac attgccttta
    10921 accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtaaaacgcg
    10981 actacaaagg cgatgacccg acgccagcgg tagggtaata accaaagggg gcgacattta
    11041 gttgccccga gattactgac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct
    11101 tatccggcct acgaaaccgc aagaattcaa tgtattgcag gagcggtgta ggcctgataa
    11161 gcgtagcgca tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc
    11221 atttatttct cacttttccg cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt
    11281 tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac
    11341 aatcaaatcg acagccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc
    11401 attgctagcg ggttaagcgc gctagcctgt tttcgatttt ttccagcgca tggcggcagc
    11461 gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtttgctgt tccacgagat
    11521 cggtcacccg cgccaggcgg gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa
    11581 aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggc gcactgtccc
    11641 actcgcgcaa tgaccaggcg gaggcttcac gcgcgatggc ttccagttgt gccgccagat
    11701 gttccgccag ccaggccact tgcggcagct gttgctgctc aactgcatga cgaagcgcag
    11761 ccagattatc gccagcctca tcgagacaag cctgcagtgt tgtcgtacgg gtttgaaaaa
    11821 ggtgtctgtc aaaacgggca cttagcgtgg caaattgcgc taccggggca caacgctgac
    11881 gcagcgtagc gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac
    11941 ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa
    12001 tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg
    12061 cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc
    12121 ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct
    12181 acgtttctgg cagaaacatc atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct
    12241 tattttgctc acgtttgcca tttctctgtg gttcgtccag atgccatggg tgcgcatcat
    12301 gttgctggta attctcgcct gtttgctttt ctatatgtgg cgaattccgg tgattgatga
    12361 aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta
    12421 cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg
    12481 atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg
    12541 actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa
    12601 cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc
    12661 agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc
    12721 gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta
    12781 ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa
    12841 tacggcaccg atcagctgga gatccacgtt gatgccatca aaccgggcga caaagttctg
    12901 gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt
    12961 cgtctgggtg gtgaagtggc tgacgctgcg ttcattatca acctgttcga tctcggcggc
    13021 gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat
    13081 taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg cattcagcct
    13141 cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc
    13201 agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg
    13261 acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta
    13321 ttcatcatgc ttatcttttt tccggtaccc gtggcgtcgg aaaaacctct atcgcccgac
    13381 tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg
    13441 ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct
    13501 cgcgcaccaa agttgaagat acccgcgacc tgctggataa cgtccagtac gctccggcgc
    13561 gtggtcgttt caaagtttat ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta
    13621 acgcactgtt aaaaaccctt gaagagccgc cggagcacgt taagtttctg ctggcgacga
    13681 ccgatccaca gaaattgccg gtgacgattt tgtcgcgctg tttgcaattt catctcaagg
    13741 cgctggatgt cgagcaaatt cgccatcaac ttgagcacat cctcaacgaa gaacatatcg
    13801 ctcacgagcc gcgggcgttg caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg
    13861 ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggcag
    13921 tcagtgcgat gctgggtacg cttgatgacg atcaggcgct gtcgctggtt gaagcgatgg
    13981 tcgaggccaa cggcgagcgc gtaatggcgc tgattaatga agccgctgcc cgtggtatcg
    14041 agtgggaagc gttgctggtg gaaatgctcg gcctgttgca tcgtattgcg atggtacaac
    14101 tttcgcctgc tgcacttggc aacgacatgg ccgccattga gctgcggatg cgtgaactgg
    14161 cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca
    14221 aagaattacc gtatgcgccg gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc
    14281 tggcgttcca tccacgtatg cctctgcctg agccagaagt gccgcgccag tcctttgcac
    14341 ccgtcgcgcc aacggcagta atgacgccaa cccaggtgcc gccgcaaccg caatcagcgc
    14401 cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc
    14461 agcagttgca gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta
    14521 cccgcgcgcg tccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc
    14581 gctggaaggc gaccactccg gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc
    14641 tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagcta gcggcagaag
    14701 ccattgagcg cgacccgtgg gcggcacggg tgagtcaact ttcgctacca aaactggtcg
    14761 aacaggtggc cttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc
    14821 gctcctctca gcggcatttg aacaaccgcg gtgcacagca aaaactggct gaagcgttga
    14881 gcacgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc
    14941 gtacgccgct ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt
    15001 ccattattgc ggataataat attcagaccc tgcgtcggtt cttcgatgcg gagctggatg
    15061 aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga
    15121 ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag
    15181 cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc
    15241 gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta
    15301 gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca
    15361 gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta
    15421 tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc
    15481 tgttaacaca gcttatggaa gcactgcgct gtctgtcggg cgttggcccg aagtcggcgc
    15541 agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg
    15601 cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcact ttcaccgaac
    15661 aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa atctgtgtgg
    15721 tggagagtcc ggcggacatc tacgccattg agcagacggg gcagttttct ggtcgttatt
    15781 ttgtgttgat ggggcatctg tcaccgctgg acggcatcgg tccggatgat atcgggcttg
    15841 atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaatc
    15901 ccacggttga aggtgaagct accgctaact acattgccga gctttgcgcg caatatgacg
    15961 tggaagctag ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg
    16021 gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga
    16081 gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct
    16141 gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt
    16201 ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat
    16261 aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt
    16321 ttccgtgcgc tatctaaccc ggacctgtac gaaggtgatg gcgaactgcg cgttcgtgtc
    16381 tctttcgata aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc
    16441 gacgaagtga ttgaccatct ggggactatc gctaaatccg gtaccaaatc attcctcgaa
    16501 tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc
    16561 tactctgcgt ttatcgtggc cgacaaagtg actgtgcgta ctcgcgcggc aggcgaaaaa
    16621 ccagaaaacg gcgtcttctg ggaatcggct ggcgaaggtg aatataccgt tgccgacatc
    16681 accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc
    16741 ctcgatgact ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat cgcgctgccg
    16801 gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg ggagaaaatc
    16861 aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa
    16921 gagttctaca aacacatcgc ccacgacttt aatgatccgc tgacctggag ccacaaccgc
    16981 gttgaaggta agcaggagta caccagcctg ctgtacatcc cgtcccaggc tccgtgggat
    17041 atgtggaacc gtgatcataa acacggcctg aaactgtacg tccagcgtgt gttcatcatg
    17101 gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc
    17161 agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac
    17221 ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac
    17281 gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg
    17341 gaagatttcg ctaaccagga agcgatcgcc aaactgctgc gttttgcttc tacccatacc
    17401 gattcttctg cgcagaccgt atctctggaa gactacgttt cccgcatgaa agaagggcag
    17461 gagaaaatct actacatcac cgcagacagc tatgcggcag cgaagagcag cccgcacctg
    17521 gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg
    17581 atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc taaagttgac
    17641 gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca
    17701 ctgactccgt tcatcgaccg tgtgaaagcc ttgctcggcg agcgcgtgaa agatgtccgt
    17761 ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc
    17821 actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc
    17881 ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga agatgaagcg
    17941 aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggcacg
    18001 ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat
    18061 gccggatgac ctttgtgtca tccggcattt ttcttttcat catctgcact ttccgcaaat
    18121 tatctcgcca ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg cctgttggtg
    18181 gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt
    18241 atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag
    18301 aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc
    18361 tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa
    18421 ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgtaa tggtttcctg
    18481 ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat
    18541 gttgattacg ttctggaatt cgacgtaccg gacgaactga ttgttgatcg tatcgtaggc
    18601 cgccgcgttc atgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta
    18661 gaaggcaaag acgacgttac cggtgaagaa ctgactaccc gtaaagacga tcaggaagaa
    18721 accgtacgta aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac
    18781 tccaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtt
    18841 gctgaagttc gcgctgatct ggaaaaaatc ctcggctaat caagccgcag cagatctgct
    18901 ttcgggcagg tctgctcatc cacctcaggc aatacctctc acagcaatta gttcttcttc
    18961 ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga
    19021 ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
    19081 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt
    19141 tatggtggcc attgctgcgc ggcgttattt tgccgctgcg ctcgccgcgt gtggcgaagc
    19201 tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac
    19261 agcaggcgct ggcacaacgt ttaccggaga cgcctgtagc gctgggaatg agctacggct
    19321 cgccatcact ggaaagcgcc gtcgatgaac tcctggcaga gcatgtagat catattgtgg
    19381 tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg gatgaactgg
    19441 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg
    19501 ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt gccaaacatg
    19561 gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat gcagatgaag
    19621 gcgatgatta cccgcaacgt tgccgcacaa cgacttgcga actggcttcc gcactgggga
    19681 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga
    19741 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt catatacagg
    19801 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
    19861 accgtgaggt cttcctcggt gccggcggga aaaaatatga atatattcca gcgcttaatg
    19921 ccacgccaga atatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag
    19981 cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc attcgtgaat
    20041 aatgcaaaaa ggcatgcagc gtacctgggt agagtttgaa ctcacagggc tgctgatgcg
    20101 ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac tccgccccgg
    20161 caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag taatacggtg
    20221 actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa tcctgttgcg
    20281 ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt aatccgtaaa
    20341 gcccatacca cagcaaaaca cccacaattt taccgcaatc gatctgttta tcacgcaacc
    20401 acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag ccaatgcggg
    20461 acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca gccacaattt
    20521 cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggg gtaatcaata ccaatcaccg
    20581 tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg agattgccga
    20641 gaataaaacc gcctccatgc aaataaaata gcgtcgccgg gctatctggc tgcggacaaa
    20701 agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg gttgccattt
    20761 ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa
    20821 tcgttcccgt tgcgggccag gacggtaaat ccggctgaag agtattcaca acggtcttca
    20881 tttcagcaga aataaggtcc agaacaggta gtttgttttc cggcttcata caaaaactcc
    20941 tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata
    21001 ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt
    21061 aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc
    21121 ctgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgccg
    21181 cctgggtcgt aggtattgac cagacgctgg tcgatattga agcgaaagtg gatgatgaat
    21241 tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat gacgtagccg
    21301 aagcgcttta tcaggagcta aaacagaaaa acctgattac ccatcagttt gcgggtggca
    21361 ccattggtaa caccatgcac aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg
    21421 gcgtcatgtg cagcaatatt gaaattggca gttatgccta tcgttacctg tgtaacactt
    21481 ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta
    21541 cgctgattgg cgagtccggg gaacgtacct ttgctatcag tccaggccac atgaaccagc
    21601 tgcgggctga aagcattccg gaagatgtga ttgccggagc ctcggcactg gttctcacct
    21661 cttatctggt gcgttgcaaa ccgggtgaac ccatgccgga agcaaccatg aaagccattg
    21721 agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggcaccaag tttgtcattg
    21781 ccgagaatcc gcagtggtgg cagcaattcc tcaaagatca cgtctctatc cttgcgatga
    21841 acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg
    21901 cgctagactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg
    21961 gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggcgctatag
    22021 cggaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc cagaatccgc
    22081 tgcgtgtata ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacta
    22141 atggagcggg ggatggcgca ttggcagcgt tgctgcatga cattaccgcc aacagctacc
    22201 atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact tattcatcgt
    22261 tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac
    22321 ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc
    22381 gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcatttggc
    22441 tctcgatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt tcatatttta
    22501 catccggcaa ccaccgttta ccccgtcacc acctcacccg ccggtggcgt ttccagcagt
    22561 tccagcatgg tacgggcgat ttcacgctcg cccatcacta cctgattcgc accacgttcg
    22621 gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat atccggattt
    22681 ttcgcgcggg cagatgccac aatctcaccc gcttcataac cgttgggaat cgtcaggatc
    22741 agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc cgcattgccc
    22801 aatactgcgc ggaccccgcg ctctcgcagc tcatcaacac gggttcgtga cgtctcaatc
    22861 accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg
    22921 taacccacca gtagcgcatg gttgcaaata tccactggga tctgcttctc ttcttcgatt
    22981 gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ctccagtagt
    23041 gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac caggttttgt
    23101 ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa cgcgaactca
    23161 ccaatctgcg ccaggctggc ggcgatggtt aatgccgtac gttgggagtg accaaacagt
    23221 cgcaccagga aaaatgcggc taacgactta ccaaacagaa taatcgccag cgtcgccagc
    23281 actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa
    23341 aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg gtgactcagt
    23401 tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac
    23461 agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc
    23521 tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg gcgacctacc
    23581 agcatcataa tggcgataaa tgcgatcact ttgccgatgg tgatccccat atcgactgca
    23641 agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg cagcaacacc
    23701 agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc
    23761 tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg
    23821 gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac ggcagagagc
    23881 gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat ggcgatggcc
    23941 tttaccgcca tcaaatcctt cagcgaaaag tgcaaaccga cgccaaacat caacagaatg
    24001 acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat
    24061 ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg
    24121 gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg
    24181 gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt ggccgggaaa
    24241 accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg aataattatt
    24301 gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa acagaaggcg
    24361 gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt ttgcctttgg
    24421 ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa catccccaat
    24481 agtggcagga aagcacagat tttatagact aactcgatgc tggtgtgatc ggcgataagc
    24541 cccagaactg ccgctcccag acctcccatg ccaaaagcaa aaccgaaaaa gagtccagaa
    24601 accataccga tacgtcccgg aagtagctcc tgagcgtaga ccagaatggc agagaatgcc
    24661 gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag gctggcgtag
    24721 ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac atatttccgt
    24781 ccaattttat cccctacagg cccgccgatc accgtacctg ccgcaacggc aaacaggaag
    24841 gcaaacagat gaagctgggc attctggata gataatccga atttttgcat cagataaaag
    24901 gtgtaatag
//
LOCUS       DAJVTY010000048        24748 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVTY010000048 DAJVTY010000000
VERSION     DAJVTY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24748
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.048"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..217)
                     /locus_tag="OQQ66_001742"
     CDS             complement(<1..217)
                     /locus_tag="OQQ66_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCS1904708.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFL"
     gene            complement(344..1075)
                     /gene="rlmB"
                     /locus_tag="OQQ66_001743"
     CDS             complement(344..1075)
                     /gene="rlmB"
                     /locus_tag="OQQ66_001743"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1904709.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1256..3697)
                     /gene="rnr"
                     /locus_tag="OQQ66_001744"
     CDS             complement(1256..3697)
                     /gene="rnr"
                     /locus_tag="OQQ66_001744"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1904710.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDGRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3736..4161)
                     /gene="nsrR"
                     /locus_tag="OQQ66_001745"
     CDS             complement(3736..4161)
                     /gene="nsrR"
                     /locus_tag="OQQ66_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1904711.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4366..5664)
                     /gene="purA"
                     /locus_tag="OQQ66_001746"
     CDS             complement(4366..5664)
                     /gene="purA"
                     /locus_tag="OQQ66_001746"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1904712.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5768..5965)
                     /locus_tag="OQQ66_001747"
     CDS             complement(5768..5965)
                     /locus_tag="OQQ66_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1904713.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6047..7051)
                     /gene="hflC"
                     /locus_tag="OQQ66_001748"
     CDS             complement(6047..7051)
                     /gene="hflC"
                     /locus_tag="OQQ66_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1904714.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7054..8313)
                     /gene="hflK"
                     /locus_tag="OQQ66_001749"
     CDS             complement(7054..8313)
                     /gene="hflK"
                     /locus_tag="OQQ66_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1904715.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8399..9679)
                     /gene="hflX"
                     /locus_tag="OQQ66_001750"
     CDS             complement(8399..9679)
                     /gene="hflX"
                     /locus_tag="OQQ66_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1904716.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9756..10064)
                     /gene="hfq"
                     /locus_tag="OQQ66_001751"
     CDS             complement(9756..10064)
                     /gene="hfq"
                     /locus_tag="OQQ66_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1904717.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10150..11100)
                     /gene="miaA"
                     /locus_tag="OQQ66_001752"
     CDS             complement(10150..11100)
                     /gene="miaA"
                     /locus_tag="OQQ66_001752"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1904718.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11093..12940)
                     /gene="mutL"
                     /locus_tag="OQQ66_001753"
     CDS             complement(11093..12940)
                     /gene="mutL"
                     /locus_tag="OQQ66_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1904719.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12950..14287)
                     /gene="amiB"
                     /locus_tag="OQQ66_001754"
     CDS             complement(12950..14287)
                     /gene="amiB"
                     /locus_tag="OQQ66_001754"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1904720.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14306..14767)
                     /gene="tsaE"
                     /locus_tag="OQQ66_001755"
     CDS             complement(14306..14767)
                     /gene="tsaE"
                     /locus_tag="OQQ66_001755"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1904721.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14739..16286)
                     /gene="nnr"
                     /locus_tag="OQQ66_001756"
     CDS             complement(14739..16286)
                     /gene="nnr"
                     /locus_tag="OQQ66_001756"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1904722.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16285..17424
                     /gene="queG"
                     /locus_tag="OQQ66_001757"
     CDS             16285..17424
                     /gene="queG"
                     /locus_tag="OQQ66_001757"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1904723.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17407..17460)
                     /locus_tag="OQQ66_001758"
     CDS             complement(17407..17460)
                     /locus_tag="OQQ66_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904724.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17689..17764)
                     /locus_tag="OQQ66_001759"
     tRNA            complement(17689..17764)
                     /locus_tag="OQQ66_001759"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Gly,seq:gcc)
     gene            complement(17801..17876)
                     /locus_tag="OQQ66_001760"
     tRNA            complement(17801..17876)
                     /locus_tag="OQQ66_001760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17841..17843),aa:Gly,seq:gcc)
     gene            complement(17913..17988)
                     /locus_tag="OQQ66_001761"
     tRNA            complement(17913..17988)
                     /locus_tag="OQQ66_001761"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Gly,seq:gcc)
     gene            complement(18199..18744)
                     /gene="orn"
                     /locus_tag="OQQ66_001762"
     CDS             complement(18199..18744)
                     /gene="orn"
                     /locus_tag="OQQ66_001762"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1904725.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18839..19891
                     /gene="rsgA"
                     /locus_tag="OQQ66_001763"
     CDS             18839..19891
                     /gene="rsgA"
                     /locus_tag="OQQ66_001763"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1904726.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNELLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19988..20956
                     /gene="psd"
                     /locus_tag="OQQ66_001764"
     CDS             19988..20956
                     /gene="psd"
                     /locus_tag="OQQ66_001764"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1904727.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20978..24301
                     /gene="mscM"
                     /locus_tag="OQQ66_001765"
     CDS             20978..24301
                     /gene="mscM"
                     /locus_tag="OQQ66_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1904728.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24452..>24748)
                     /locus_tag="OQQ66_001766"
     CDS             complement(24452..>24748)
                     /locus_tag="OQQ66_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1904729.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5697 a         6912 c         6291 g         5848 t
ORIGIN      
        1 ataagaaggt attaaattca tctggattgc tgatactact taccggacaa ataaaggttt
       61 cgataatcat ttggcgggaa gtaaaaagaa tattgatttg caaatcgcca taatcattca
      121 ttttaataat taatgcgttc tcgctatttg ttacatcaat attttgttca ggtacagttt
      181 gcagcgccgt cgccagcgcc aacagattcc atgtcatata ttattccata tagatttagt
      241 ttaaatatta ataaaatgaa tatttgcagt acgtaattat cttaccagct atagacaaaa
      301 aaaaaccatc caaatctgga tggcttttca taattcagag gaactagctg cgctgacgca
      361 ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt cagagaagaa acacttcctg
      421 ccattgggat gctgatcaac tcatcgcaat gttcacgggt caggcgacgc ataccttcac
      481 cttccgcgcc catcaccagc gccaggcgtc cggtcatttt gctctgatag agtgtatgat
      541 ccgcctcacc tgccgtaccg acgatccaga tattctcttc ctgcaacata cgcatggtac
      601 gcaccaggtt agtcacccga atcagcggaa cgctttctgc cgcgccacag gccacttttt
      661 tcgccgtggc gttgagctgg gcggagcgat ctttcggcac aatcaccgca tgaacaccag
      721 cggcatctgc gctacgcagg cacgcgccga ggttatgcgg atcggttaca ccgtcaagga
      781 tcagcaggaa cggctgatcg agcgaagcga tcagatccgg caaatcattt tcctggtact
      841 gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt
      901 cgagatattg gcggtttgcc agctggataa ccacgccctg ggcttcgagg gcgtgaatca
      961 gcggtaacag acgtttatct tcacggcctt ttaaaataaa gacttcctga aaacgttcag
     1021 gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta aatcatttcg ctcattaatg
     1081 tactcgttgt ttacgttttg cctgatgcac tacgtttatc cggcctacat gatctctgca
     1141 atatattgaa tttgcgtgat tatgtaggcc ggataaggcg ttcacgccgc atccggcatg
     1201 aacaaagcgc attttgtcag caatctaacc ctcttctgtt tgaagagggt aatgatcact
     1261 ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc tgcagctatt ttctgcgttt
     1321 tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc tgctttcggc ttcgtttttt
     1381 tctcaccgcg gaaggcgctg tctggctcaa agtttacctt tttaccgacc agacgacgct
     1441 tgccgccttt tttacctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt
     1501 tgcgcggtgc gcgttcgctg gagatcaggc taaagtcgat tttgcgctcg tccatattaa
     1561 ccgcttcgac gcgaacttcc acgcgatcgc ccagacgata agtctggccg ctggattcac
     1621 ccatcagccg ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat
     1681 ggaccagacc atcaatgaat aagtcgtcca gacggacaaa gaatccaaag ccagtgacgc
     1741 tggaaattac gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc
     1801 agtcagccac atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac
     1861 ccagttgcag catttcttcc atcgaataat ggtagccgcc ggtttcagtg gtgttgccct
     1921 gatgcccctg ctctttcgcc agcagatatt taatggcgcg gtgcagcgtc aggtctggat
     1981 aacgacgaat cggcgaagta aagtgcgcat aggactgcaa tgccaggcca aagtgaccac
     2041 ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag cagcatggtt tgcagcattt
     2101 ctgcatcagg acgatcggca accgactcca gcagctccgc gtagtcacgc ggttccggct
     2161 tgttaccgcc cggcagttcc agccccagct ccgccagtac tgaacggaaa gaggtaatcg
     2221 cttcggtgct cggcttgtcg tgaatacgga acaatgctgg ttctttggct ttctcaacga
     2281 aacgcgccgc cgagatattc gccagaatca tgcactcttc aattaacttg tgtgcgtcgt
     2341 tacgctgagt ctgttcgata cgctcaatac ggcattcagc gttgaaaata aacttcgctt
     2401 cttcgctctc aaatgagatc ccgccacgtt cttcacgggc tttatccagc actttataga
     2461 ggttatgcaa ctcttcgaga tgcttaacca gcggagcgta ctgctcgcgc agatcctgat
     2521 cgccctgcag aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg
     2581 cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca
     2641 tacacaggcg gtctacctgc ggattgagcg agcacagacc gttagagagc acttccggca
     2701 gcatagggat aacctgcgaa gggaagtaca ccgacgtacc acgattacgc gcttctctat
     2761 ccagcggcgt tggcggacgc acatagtagc tgacgtcggc aatcgcgacc cataaacgcc
     2821 agccgccgcc gcgttttttc tcgcagtaaa ccgcatcgtc aaagtcacgg gcgtcttcgc
     2881 catcaatggt gaccagcggt aaatcgcgca aatcgacacg gcccgctttt gcttcttccg
     2941 gcacttcttc tttcagcccg gcaacctgtt gctcaacagc ctgcggccag atgtacggaa
     3001 tttcatgggt acgcaaagcg atatcaaccg ccatgccggt gcccatattg tcgcccagca
     3061 cttcgacgat tttacccacc gctttggtac ggcgagtcgg gcgttgggtc agttcaacaa
     3121 ccaccacaaa gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc
     3181 tcagacggcc atcgtcagga accacaaagc cgacgcccgc ttcggtaaag tagcgaccaa
     3241 caatctggct ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac
     3301 ggtcagcacc cagcggctga gccagcacct gatcgccatg aatgcaggtt ttcatctgct
     3361 cgctggagag atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac
     3421 ggtggccaat aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac
     3481 gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt
     3541 caagctgctc ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt
     3601 tttcacgttt ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt
     3661 tttcagcttc gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc
     3721 tgatgaagat tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac
     3781 caaatcggca agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag
     3841 cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag
     3901 cgataagggc tccagctcgc gcaccacatc accaatacgt atcgcactcg ccggtttacc
     3961 caggcgaatg ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg
     4021 attgattatt ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat
     4081 actggtcatc cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc
     4141 agtgaaactc gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat
     4201 attgatgata aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa
     4261 aagcgaggat ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc
     4321 acactgttag cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg
     4381 gtcgcgcaga atcatagttt cagtacgatc cggaccggta gagatgatat cgatcggcac
     4441 accggtcagc tcttcaatac gcttgatgta gttcagcgcc gcctgcggca gcccgctacg
     4501 atctttcacg ccgaaagtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac
     4561 acctttccag tcgtcagctg ccagcggagt ggtagtcact tcgcgaccat ccggcatacg
     4621 gtaagccacg cagagtttaa cctctttcag gccatccaga acgtccagtt tagtcaggca
     4681 gaagccagac agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc
     4741 ggtacgacga cgacgacccg tcgttgcgcc gaattcgtta ccctgcttgc agaggaactc
     4801 gccagtttca tcaaacagtt cggtcgggaa aggacctgca cccacacgag tggagtaagc
     4861 tttgaggata cccagaacgt aatcaacata acgcgggccc aggccggaac cggtcgccac
     4921 gccaccagca gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag
     4981 cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca cgctgacgcg cctggtcgag
     5041 caggtcagaa acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc
     5101 cagaactttc tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg
     5161 atattccatc acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac
     5221 acgcagaccg cgacgtgcca ctttatcttc ataagcaggc ccgataccac gaccggtggt
     5281 gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta tccagcgcaa cgtgataatc
     5341 aaggatcagc ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc
     5401 ttccagttct ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat
     5461 gatgctggtt acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc
     5521 accgttgatt acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt
     5581 agctcgttca gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc
     5641 cagtacgacg acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta
     5701 ccatcgcttt ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt
     5761 tttttgttca gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa
     5821 gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct
     5881 tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa
     5941 gcgccagcca gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat
     6001 ccctgaggat gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg
     6061 aagtcggcgt cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct
     6121 gattgccaga gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg
     6181 gatctttact gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca
     6241 tgatgcggcc ctgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg
     6301 cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac
     6361 gctcggcgcg catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct
     6421 tgatacgcac atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg
     6481 gaactttgcc cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatctg
     6541 ctgccggggt agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta
     6601 cttcgagggt cagacgacca cgggaatcag tgacgatatc tttcacgtcc aggcgaccaa
     6661 tttcagaacg cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt
     6721 cgccaccacc cgttgccagg tagtaacggc tgaaatcgct gatgcgccat ttgatgtaag
     6781 agtcgacgat caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg
     6841 tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac
     6901 ccggctcata aaccagaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg
     6961 taataccgcg ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga
     7021 tgatgataat cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt
     7081 acgctgcgcg ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt
     7141 gcttgctcca ctggttgtgg aggaagaggc tggcggcaaa cgcagcagat tgctggcacc
     7201 gttatcgctc ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac
     7261 catcaggttg ccacctttat cgttaaccag cactttgcgg gtgttaccca acactttttc
     7321 catcgtctcg atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag
     7381 aagtttagca aagcgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc
     7441 acgcgcctct tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt
     7501 atacgcttct gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc
     7561 aaacgccgct tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagagt
     7621 gatgcccatg tcatacggac gaatcgtctc ttccagttcg cgctgggtat cgctacgaat
     7681 cacggtacga ccttccgtca gaatgcggtc catggtgtat ttaccgataa ccccgcgcag
     7741 ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt
     7801 ttccggatag gtaacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga
     7861 cgtcagcatc acaccggaag cggccagttc acgcacggct tccacgttta ccggtttgac
     7921 ttcgtcgata aacgtcggtt tccagttcag acccggctca accagatggc tgaatttacc
     7981 aaagcgtgtt accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca
     8041 gataatgaca atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg
     8101 gccttgcgat gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag
     8161 ctttttgctc agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc
     8221 tttgtttcca tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc
     8281 ttgtccgtta ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt
     8341 gatccctgtt gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt
     8401 agatcaggta atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg
     8461 gcatacgaac ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct
     8521 gaagctgata aaaacgactt ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct
     8581 gcgccacctc gccggaaagc cgctccgtca aagcctgaaa aagctgtggt atccccgctc
     8641 cggtctgtgc ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg
     8701 gttcgaaatc ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag
     8761 cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg
     8821 ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa
     8881 atgccgccac cagatcgtgc ggcagatggc gaataaaccc tacggtatct gcaagtacgg
     8941 tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact
     9001 ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt
     9061 tggtatatcc caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct
     9121 gctcacgctg cttttcaact ctttccaggc gcgactgtat ctgcacgatg cgattacgca
     9181 acaaacgacg gtcggtttcg agctgggttt cacccggacc acgcaaacct atcccgcctt
     9241 tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt agccagatgg cgcagctgcg
     9301 ccagctcaac ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa
     9361 taaggccggt gcggtcgatg acacgacact cgcacaaacg ctccaggtta cgctcttgcg
     9421 ccgggctcag ggcatggtca aaaagaacga ccgaagcacc cgtcgctttg acagcttccg
     9481 caatttcaac tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac
     9541 cggtaatcac ctgcaatgct tcgacaccgg cggaagaaac cagagattca aactcctgga
     9601 ggtcttccat atctttgtct ttcgtaaaat agatgtgtac cagtaccgcc tgctcaccag
     9661 catcataacg gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc
     9721 gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt cggtttcttc gctgtcctgt
     9781 tgcgcggaag tattctgcgc gctgctacca tgatggtagt tactgctggt accgccaccg
     9841 gcgttgttac tgtgatgaga aaccggacga gacgggacaa cagtagaaat cgcgtgcttg
     9901 taaaccatct ggctgaccgt gtttttcaac aggatcacga actgatcaaa agactcgatt
     9961 tgcccttgca gcttaatacc attcaccaaa taaatagaaa ctggaacacg ttcccgacgc
    10021 agtgcgttca ggaacggatc ttgtaaagat tgccccttag ccattctctc ttttccttat
    10081 atgcttattt gtactttgaa cctttcgatt ctgaaaaaat tgcgcacgat acgtctcaat
    10141 tgtacacatt cagcctgcga tagcaccaac aacctgtaat acttcgtcac gcgcctgttc
    10201 tggtttttca ctgtcaagcc agtgaacccc ttcccaacca cgcagccagg ttatctgccg
    10261 cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc atttcatcgt atgagatttc
    10321 gccttcaagg taagaccaca tctggcgata acccacgcaa cgaatggaag gcaagtccgt
    10381 atgcaaatct cctcgggcaa aaagcgcccg gacttctgct tcaaaacctg aagccaacat
    10441 ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca cggctcgccg gggcgatggc
    10501 gaactgatgc acctgatacg gtagagcgtc tcctgacgtt tgcgtcagtt ccgttaaagt
    10561 tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc ctttgtggat catttggatg
    10621 aatccttgct gccgcaaccg gatctacctc ctgaagttga cgatgcaatg actcccaacc
    10681 ttgctctgcc gcctgttgct caattctggc ccgcacttcc gggtctgccg acggtagcgg
    10741 cgacaaccct tccagcaatg ccttgaaata caacatcgta ccgcccacta acagtgggat
    10801 ccgccccgcc gcggtgatat cggccatttc cgccagcgca tcgcggcgaa aatcagcagc
    10861 cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg tgcggcgcgg cgagtaactc
    10921 ttcagcgttc ggcttcgccg tcccgatatc catccctttg taaataaggg cagaatcaac
    10981 gcttatcaac tctactggta aaattttacg cagctcaatg gctaacgccg ttttaccgga
    11041 ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc gccttactga tatcactcat
    11101 ctttcagggc ttttatcgcc ggatgtaaat caacagattg taacagacca cccggcggcg
    11161 ttttcacaag ttgcggacat aaccgttcca cgtccgccag cagggttatg gcctgtgcca
    11221 ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat ccactgcgca atattgccag
    11281 gttcgaatac ggactgcttc gccaggtagc ctatcagttc aggaatcaag atttgtaaat
    11341 tttgttggcg taagggtaaa ggcacggccc tgatggtcac atgctgtgca tctgactgga
    11401 aatcaatacc caattccgcc agggcagact gcgctttttc taatgccgat ttttcttcgc
    11461 cagaaacttt tagccgcaac ggaataagca gcggctgagc gcaaactggc gcttcacccg
    11521 gggtcagttg tacctgacgc agccaacgtt ctgccactgg caaggctaaa agtgaaatgt
    11581 tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat agtcagtacc cgaccaaaac
    11641 tctgactgtt cgccgcaagt gcaggttcct gcggttccgg cgcttttaat ttttgcatcg
    11701 gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg ctgtttctgg tagcctggct
    11761 gcgcattcgg ccaaggggca gccggacgac tgcctgatgc tggcgcagga gtgtagcgcg
    11821 gagctaccgg ctcacgagct gccggttctg caaagtgatt gcgccccgcc gccacgcggt
    11881 tttccggaat ggaacgcggt gcaggttggg gttcatcgtc cagcggtagc ggcgtttcca
    11941 gttgctgttg tagcacgctc agcacgccct gatagataaa atcatgcacc agacgcgact
    12001 gatggaaacg cacttcgtgt ttggcggggt gcacgttgac gtccacctga tgcggatcga
    12061 tctccagata cagcacaaat gccggttgct gatcggcccc cagtttgtct tcgcaggcct
    12121 ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg accgttcacg tagcaatact
    12181 gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc cacccagccg cgtagcgtga
    12241 gatcgccgtg ttgccattca atcgccagcg cttgttcaag aaaagcggtg ccgcaaatcg
    12301 cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc gcggtactga cgcacaattt
    12361 taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc cagcgcaatg cggcggatga
    12421 tctcatcaat gtggttaaat tcggttttct cggtgcgcag gaatttgcgc cgcgccgggg
    12481 tgttgtagaa cagatccagc acctccagcg tcgtccccac aggatgcgcc gccggtttta
    12541 ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca ggcttcctgc tgttctgcgg
    12601 tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact cgccagcgcc tcaccgcgaa
    12661 agcccaggct gataatggct tcgagatcgt ccagagaggc gattttactg gtggcatgac
    12721 gagccagcgc cagcgccagc tcatcttttt tgataccgca gccgttatca cgaatgcgga
    12781 taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg cgtcgcacct gcatcgaggc
    12841 tgttttccac cagttctttg actaccgacg caggtcgctc gaccacctca cctgcggcaa
    12901 tctggttcgc cagttgtggc ggtaagacct gaattggcat caatcgtcct tagtttggca
    12961 gcgtgcgatc tggcgtcgtc accgtactgg ccgtttgtgc cgttgcaccc tgcggcgcag
    13021 attgcatcgg atgcgcaagg aaataattgc gcaggccttt gtaaatggct tctgccagct
    13081 gttgttggta atcgtcgctc gccagcaaac gttcttcgct gttgttgctg ataaaaccgg
    13141 tttcgaccag tactgacggg atatccggcg aacgcagaac gccgagactc gcgtgttctg
    13201 gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact gatcatactg gtcgctacat
    13261 cataccctac ccgctgggaa tgaccgaact gtaaatccag caccgcctgg cttaaatagg
    13321 gatcagactg actgttcgcc agcacatcgc ccgccccacc cagcagctcc gaatgtttct
    13381 cgtgctgctc cagccagctg gccatttcgc tgttggcgcg acggttagag agcacccata
    13441 cggaagcgcc agtcgcactg cggttcggtg cggcatcagc gtgaatcgac acgaggaaat
    13501 tagcgttttg cttacgtgcc acatcgctgc gccccatcac cgaaataaag taatccccgt
    13561 cacgggttaa aacgccttta aacatcggat cgtcattgag caaagtacgc aatttacgcg
    13621 cgatggcgat ggtgacattt ttctcccgcg taccaccggg gccgatagcg ccagggtcct
    13681 gaccgccgtg tccggcatca atagcgatga taattttatc gccagtgtta gccgtcgcgc
    13741 gcgctgccgg acgcgttacc gtattactgc tgataacacc cgtagtgcgg ttactttccg
    13801 ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc gacaaccgca ggcgtttcaa
    13861 cgcgtttcgc aaccacgggc ggcggaggag gcggtggcgg cacatcggcg ttaatcgtaa
    13921 agacgacagt gtaattgctg ccattctgcc gcttcaccgc ttcggtttta ccattttcgg
    13981 taagatcgac caccagccgt agcgtttgtg catctttagg cgttccagag cgaatcgcct
    14041 tcaccagatt attgccgctg aacaacaacg gcagtccctg aatcacgccc gtttgtttga
    14101 tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc ataatcagga tcgccaataa
    14161 aactcaacgt tatccgcgcc tgttgattac cgttagaaac ctgaatatca gagagcgtcg
    14221 cggcacccac cggcgtgcac agcagcagca gcgtcgctac caaccaattt ctgatgcgat
    14281 acatcatccc gccacctttc aaaggttaac cggctaaacg cgccagcaac aattcacccg
    14341 cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt
    14401 cgacatccgg gtcaggaaga acacctgtac cttgttgtgg ccactccacc aggcagatgg
    14461 catcgttggc aaaataatcg cggatcccca taaactccag ctcctcggga tcggcaaggc
    14521 ggtacaaatc aaagtgataa accattaaat tgtcgagcgt atagggttcg accagcgtat
    14581 aagtggggct tttgacatta ccctgatgac ccagagcttg taaaaagccc cggctaaagg
    14641 tggtcttacc tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt
    14701 tcgctacccg ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat
    14761 tcatcatggt ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga
    14821 tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgccgcaccg
    14881 tgcgcgacac agcccgcaca ggcggcatca tacggcgaca gtttttgccc aagcaatgcg
    14941 ccaataatac cagagagcac atcgcccatg ccgccgctcg ccatgcctgc atttccggca
    15001 tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc
    15061 gctacgccgc cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt
    15121 tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga
    15181 ttgtgacgct tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc
    15241 ggtttgcgaa aattctcaac tttttgcagt gcttttttcc cccactcttg ctggcccaga
    15301 ccgggaccaa tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatcgtc
    15361 agttcatgca ccatcaattc cggtcgtgca gtcagcagcg gcgcaatgtt ttcactgcgg
    15421 gtcagtactc ggactaaacc agcaccagca cgcagcgccg cttcccccgt catacgaata
    15481 gcccccgccg tgccgtgatc gccaccgata attaccagcc gcccgtgatc gcctttatgc
    15541 gaagtcgggc gacgcggttt tagccagtga gaaagttgtt ctgctgaaaa ccgctgaatt
    15601 ttcgtctcct gacctgccag ccaactatcc agccccagtg agtcaaaatg cagttgtccg
    15661 gtaacatccc gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg
    15721 tgatcggcgt tgatcaccgc gcctggcgta gcgccagttt cagccagcag gccggaaggg
    15781 atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg
    15841 gattcgcggg gcgcttgccg caaaccggta ccgagcagcg catcaacaat cagatctacc
    15901 gattcgggcc agacaatatt cgaagcatgg atctcgccac ccgcgtttaa ccatgcttcg
    15961 cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct cctgggcaaa caacgtgacc
    16021 tcaatgccga ccgctttggc cagtcgcgcg accacgtagc catcgccgcc gttattacca
    16081 tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg ccgaacgaca cacctggaat
    16141 gcggcctcgc cagcgcgaag catcagctca tagagtgtga gccccagcac atctgccgcc
    16201 tcgcgttctc cgcggcggat atcgtcggcg taccagacgg tgtgtggtat acttacgggg
    16261 tttttcttca ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa
    16321 aaaattaaac agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat
    16381 ctcagcgagt ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg
    16441 gactggatgg cacgtcacgg tatgctgcgc gctcgccctc atgagttatt gcccggtacg
    16501 ctgcgcgtga tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg
    16561 ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa
    16621 cttctgcgca accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg
    16681 aattttagac cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgaaaaagct
    16741 gggctcggct ggacaggtaa gcactcactt atcctcaatc gcgaggccgg ttcgttcttc
    16801 tttttaggcg aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga
    16861 tgcggtaaat gcgtggcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat
    16921 accgtcgatg ctcgccgctg tatctcttat ctcaccatcg aacttgaagg ggcgatcccg
    16981 gaagagttgc gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcctatc
    17041 tgcccgtgga atcgctattc acaactcacc acagaagagg atttcagccc gcgtaagccg
    17101 ctacacgcac cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc
    17161 acggaaggat cggcgattcg tcgaattggt cacctgcgtt ggctgcgtaa tatcgccgta
    17221 gcattaggca atgccccttg ggatgaaacg attttgacag cgctggaaag tcgtaaaggt
    17281 gagcacccac ttctcgatga gcacatagcg tgggcgattg cgcagcaaat agagagacga
    17341 aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa
    17401 aaagggttac cgcgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat
    17461 tgcaattcaa gacgaataaa atcgtcaagt gatcgactcg acaaaacgga atcttaattt
    17521 ttaacttatc tatcaatagg ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat
    17581 taagattaca gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacga
    17641 aaaccagcaa taacgcatcg ctgtggatat tttattgaga gaagaatttg gagcgggaaa
    17701 cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc
    17761 ccgcttgggt ggtctgtgcc ttacagcact tttcaaattt tggagcggga aacgagactc
    17821 gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat tcccgcttgg
    17881 gtggtctgtg ctttgcagca cttttcaaat tttggagcgg gaaacgagac tcgaactcgc
    17941 gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcgt actacttaat
    18001 tttgctttcg taattttcgc atttctgcgt cgttacggga ggcgtattat acgagaaatc
    18061 tttccctctg caacccccca aaaaagtttt ttttgcgttt ttatctcaag tgctgaaatt
    18121 atcagcaaga cgttcaattt agcgtcaaaa tcacgccgcc gcaagtggat tccggcgctt
    18181 acgtgaccag gaaaaatttt acagcttgat aaaatgctcg cggtagtaag ccagctccgc
    18241 caccgattca cggatatcat ccatcgcctg atgcgtcccc tgcttggtaa aactatccag
    18301 aatttccggc ttccagcggc gcgccagctc tttcagggtg ctgacatcga gataacggta
    18361 gtggaagtag gcttccagct ccggcatgta tttaaacagg aaacgacggt cctgtccgat
    18421 gctgttaccg caaatcggcg attttcccgc aggcacccac tgttttaaga attcgagcgt
    18481 tgccagttca gcttcccgat cgcccatcgt gctcgctttc acgcgctcta ccagcccgct
    18541 ggcggtatgg gtgcgcacgt tccagtcatc catcagcgcc agctgttcat cggactgatg
    18601 tactgcaatg gtcggccctt ctgccagaat attcaggttg gcatcggtca ccagcgtggc
    18661 aatctcaata atgcgatcgc gctcgggatc cagaccagtc atctcaagat cgatccaaat
    18721 caggttgttt tcattggcac tcatgctatt ttccacccat tcacagtgac tatattgatc
    18781 cacaattgcg tgtatcatag atgttttgcc catcaggggc gaccaggagt ctgtacgatt
    18841 gagtaaaaat aaactctcca aaggccagca gcgccgcgtg aacgccaatc accagcgtcg
    18901 tcttaaaacg tctaaggaga agcccgacta cgacgacaat ctgtttggcg agcctgatga
    18961 aggtatcgtc atcagccgct ttggtatgca cgctgatgtg gaatccgccg atggcgacgt
    19021 tcaccgctgc aatattcgcc gtaccatccg ttcgctggtg accggcgacc gtgtagtctg
    19081 gcgtccgggt aaaccggcgg cggaaggcgt aaatgtcaaa gggatcgtgg aagcggtgca
    19141 tgagcgtacc tcggtgttga cgcgtccgga tttctacgac ggcgtaaaac ctattgccgc
    19201 caacatcgac cagattgtca ttgtctccgc cattttgccg gagctgtcac tcaatattat
    19261 cgaccgttac ctggtggcct gcgaaacctt gcagattgag ccgattattg tgctcaacaa
    19321 gatagacctg ctggacgacg aaggcatggc gttcgtcaac gagcagatgg atatctaccg
    19381 caatatcggt tatcgcgtat tgatggtttc cagccatact caggatgggc taaaaccgct
    19441 ggaagaggcg ttgaccgggc gcatcagcat ttttgccggg cagtctggcg tcggcaaatc
    19501 cagcctgctg aatgagttgc tggggctgca aaaagagatc ctgaccaacg atgtctctga
    19561 caactcgggg ctcggtcagc acaccacgac cgccgctcgg ctgtatcact tcccgcacgg
    19621 cggtgatgtg attgattccc ctggtgtgcg tgagttcggc ctctggcacc tggagccgga
    19681 acaaatcact cagggctttg tcgaattcca tgactattta ggtctgtgta aatatcgcga
    19741 ttgcaaacac gataccgatc cgggctgcgc tatccgggaa gcggttgagg aagggaaaat
    19801 cgcggaaacc cgtttcgaaa actatcaccg tattctggaa agcatggcgc aggtaaaaac
    19861 gcgtaaaaac ttttctgata cggatgactg acaattaagc taaccgtcgt tagaatcgtc
    19921 cccttttttt caggatccgg catgttggcc ggatcaggaa cgacaaaaca atggcctgga
    19981 ggctaccttg ttaaattcat ttaaactttc gctacagtac attctgccga aactatggct
    20041 tactcgcctg gcgggttggg gcgcaagcaa gcgggcagga tggctgacaa aactggttat
    20101 cgatctgttc gttaaatact acaaggtcga catgaaagag gcgcaaaagc cggacaccgc
    20161 cagctaccgc acctttaacg aattctttgt ccgtccgctg cgtgacgaag tacgcccaat
    20221 cgataccgat ccgaatgtac tggtcatgcc tgccgatggc gttatcagcc agttgggtaa
    20281 aatcgaagaa gataaaatcc tgcaagccaa aggccacaac tacagcctcg aagccctgct
    20341 ggcaggcaac tatctgatgg cggacctgtt ccgcaacggt acgtttgtga ccacttacct
    20401 ctccccgcgt gactaccatc gcgtacacat gccgtgcaac ggtattctgc gtgagatgat
    20461 ctacgtgccg ggcgatctct tctccgttaa ccatctcacc gctcagaacg tgccgaatct
    20521 gtttgcccgt aacgaacgcg tgatttgcct tttcgatacc gaatttggcc caatggcgca
    20581 gattctggtc ggagcgacga ttgttggcag cattgagacg gtctgggcgg gcaccattac
    20641 gccgccgcgc gaaggtatca tcaagcgctg gacctggcct gccggggaaa acgacggttc
    20701 tgtggcactg ctgaaaggcc aggaaatggg tcgctttaaa ctcggttcca ccgttatcaa
    20761 cctgtttgca ccgggtaaag tgaatctggt tgagcaactg gaaagcctgt ctgtcacgaa
    20821 aattggccag ccgctggcag tatctaccga aacctttgtt acgccagacg ctgaacccgc
    20881 cccacttcct gctgaagaga tcgaagcaga acacgacgcc agcccattgg ttgacgataa
    20941 aaaagaccag gtctaatcca tcaaaggaaa cgctgacgtg cgcctgatta tcacttttct
    21001 gatggcctgg tgcctcagtt ggggggcgta cgccgcgacg gcccccgata gcaaacaaat
    21061 cactcaggaa ctggagcagg caaaagcggc gaaacccgca cagccggaag tcgtagaggc
    21121 gctccagtct gccttaaatg cgcttgagga acgaaaaggt tcccttgagc gcatcaaaca
    21181 atatcagcaa gttatcgata attatccgaa actctccgct actctgcgcg cacaattaaa
    21241 caacatgcgt gacgagccgc gcagcgtgtc gccgggaatg tctaccgacg cgctgaatca
    21301 ggaaattctc caggtcagca gccagttgct ggataaaagc cgtcaggccc agcaagagca
    21361 ggagcgcacc cgcgagattg ccgattcgct gaatcaactg ccgcaacagc aaaccgacgc
    21421 ccgccgtcag ttaaatgaga tcgagcgccg cctgggaacg cttaccggca atactccgct
    21481 caatcaggca caaaatttcg cgttgcagtc tgactctgca cgtcttaagg cgctcgttga
    21541 tgaactggag ctggcgcagc tgtctgccaa taaccgccag gaattagcgc gcttacgctc
    21601 agagctggcg gaaaaagaga gccagcaact ggatgcgtat ttgcaggcct tgcgtaatca
    21661 attaaacagc caacgtcagc ttgaggcgga gcgggcgctg gaaagtaccg aattgctggc
    21721 agaaaacagc gccgatttgc cgaaagatat cgtcgcgcaa ttcaaaatta accgcgaact
    21781 atcggcggct ttgaatcaac aggcgcagcg gatggatctc gttgcctcgc aacagcgtca
    21841 ggctgccagc cagacgttac aggtccggca ggcgttgaat acgctgcgtg aacagtcgca
    21901 atggctggga tcgtccaatc tgctcggcga agcgctgcgg gcgcaggtgg cacggctgcc
    21961 agaaatgcca aaaccacaac agcttgatac cgaaatggcg cagttgcgtg tgcaacggtt
    22021 acgttatgag gatctgctta ataaacagcc gctgctacgg caaatccatc aggccgacgg
    22081 tcagccgctg accgccgagc aaaaccgtat tctggaagca caactgcgca ctcagcgtga
    22141 gttgctgaac tcattgttgc agggtggcga cacgctacta ttggaactga ccaagctgaa
    22201 agtctccaac gggcaactgg aggatgcgct gaaagaggtg aacgaagcaa cgcaccgcta
    22261 tctgttctgg acctctgacg tgcgcccgat gaccatcgcc tggccgctgg aaatcgccca
    22321 ggatctgcgt cgtctcattt cgctggacac cttcagtcag ttgggcaaag ccagtgtgat
    22381 gatgctgacc agcaaagaga caattttgcc gctgtttggc gcgttgattc tggtcggttg
    22441 cagtatttac tcgcgccgct atttcacccg ttttcttgaa cgttcggcgg cgaaagttgg
    22501 caaagtgact caggatcact tctggctgac gttgcgcact cttttctggt cgattctcgt
    22561 cgcgtcaccg ttaccggtgc tgtggatgac gctgggttac ggcttgcgcg aggcgtggcc
    22621 ttatccgctg gcggtcgcga ttggcgatgg tgtaacggct accgtgccgc tgctgtgggt
    22681 agtgatgatt tgcgccacct ttgcccgccc gaacggcttg tttatcgctc attttggctg
    22741 gccgcgcgaa cgtgtttccc gtgggatgcg ctactacctg atgagcatcg ggcttattgt
    22801 gccgctgatt atggcgctga tgatgttcga taacctcgac gaccgtgaat tctccggttc
    22861 gctgggacgg ctttgcttta tcctcatttg cggtgcgctg gcggtggtca ccctcagcct
    22921 gaaaaaggcc gggatcccac tgtatctcaa caaagagggc agcggcgaca acattaccaa
    22981 ccatatgctg tggaacatga tgattggcgc gccgttggtt gccattctgg cgtcggctgt
    23041 gggttatctg gcaacggcac aggcgctgtt agcgaggctt gaaacctcgg ttgccatctg
    23101 gttcctgcta ctggtggttt atcacgttat ccgccgctgg atgctgatcc agcgccgcag
    23161 gctggcgttt gaccgggcga agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg
    23221 cgaagaggaa gcgcatcatc acagtagccc ggaaggagca attgaagtcg atgaaagcga
    23281 agtcgatctc gatgccatca gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt
    23341 gatcgccctg ctttctgtca ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct
    23401 cgaaaatatt tcgctgtggg atgtcacctc cacggtacaa ggcgtagaaa gtctggagcc
    23461 aattaccctc ggtgcggtgc tgattgccat tctggtgttt atcatcacca cgcagctggt
    23521 gcgcaacctg cccgcgctgc tggaactggc gattttgcag cacctggatt taacgccggg
    23581 tacaggctac gccatcacca ccatcaccaa atatctgctg atgctgattg gcgggctggt
    23641 cggcttctcg atgattggta ttgaatggtc gaaattgcag tggctggtcg ccgcgctcgg
    23701 tgttggtctc ggttttggtt tgcaggaaat tttcgccaac tttatctctg gtctgattat
    23761 cctgttcgaa aaaccgattc gcattggcga tacggtgaca attcgcgatc tcaccggtag
    23821 cgtgacgaaa attaacaccc gcgccaccac catcagcgac tgggaccgta aagagataat
    23881 cgtgccgaac aaggcgttta ttaccgagca gtttatcaac tggtcgctct ctgactcggt
    23941 tacgcgcgta gtgttgacga ttccggcccc tgctgatgcc aacagtgaag aagtgacgga
    24001 aatcctgctc accgcagcgc gtcgctgctc gctggtgatc gacaacccgg caccggaagt
    24061 cttcctggtg gatctgcaac aagggattca gattttcgag ctgcgtattt atgccgctga
    24121 gatgggtcac cgtatgccgc tacgccatga gatccaccag ctgattctgg ctggcttcca
    24181 tgcccacggt atcgatatgc cattcccgcc cttccagatg cgtctggaaa gcctcaacgg
    24241 taaacaaacg gggagaacgc tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta
    24301 atgcatgaag ctgccaaatg atgtcaacgt ggctcattta gcagcttaac atcatcttca
    24361 gttatcaccg gccctggaga gtatcatcca tacgcattac ttcctgacaa ctcatcgccg
    24421 gagagacgat gagttgcagg atttcatatg atcagtcgag agctaactca ggtttattcg
    24481 ccatgcgact gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag aagacagggc
    24541 cgccaatcat ccacaatgtg ctgtcccagt ctccggcttc aaccacaggc ggaataatgg
    24601 tgaagacgtt cgcaaatgtc accaccagta cgacaaccac cgtcgcaatc attgccgaca
    24661 tacgcgtttt gaaaatcaca aatggtctgt cgagatcctg acgtgcttta aagaatggga
    24721 aagccagcgc gaggaacagg taaggaag
//
LOCUS       DAJVTY010000049        24054 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVTY010000049 DAJVTY010000000
VERSION     DAJVTY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24054
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.049"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            316..543
                     /gene="feoA"
                     /locus_tag="OQQ66_001767"
     CDS             316..543
                     /gene="feoA"
                     /locus_tag="OQQ66_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1904730.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            560..2881
                     /gene="feoB"
                     /locus_tag="OQQ66_001768"
     CDS             560..2881
                     /gene="feoB"
                     /locus_tag="OQQ66_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1904731.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2881..3117
                     /gene="feoC"
                     /locus_tag="OQQ66_001769"
     CDS             2881..3117
                     /gene="feoC"
                     /locus_tag="OQQ66_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1904732.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3320..4198
                     /gene="rpnA"
                     /locus_tag="OQQ66_001770"
     CDS             3320..4198
                     /gene="rpnA"
                     /locus_tag="OQQ66_001770"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1904733.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4227..4997)
                     /gene="bioH"
                     /locus_tag="OQQ66_001771"
     CDS             complement(4227..4997)
                     /gene="bioH"
                     /locus_tag="OQQ66_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1904734.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5035..5718
                     /gene="gntX"
                     /locus_tag="OQQ66_001772"
     CDS             5035..5718
                     /gene="gntX"
                     /locus_tag="OQQ66_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1904735.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5777..6352
                     /gene="nfuA"
                     /locus_tag="OQQ66_001773"
     CDS             5777..6352
                     /gene="nfuA"
                     /locus_tag="OQQ66_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1904736.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6713..8029
                     /gene="gntT"
                     /locus_tag="OQQ66_001774"
     CDS             6713..8029
                     /gene="gntT"
                     /locus_tag="OQQ66_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1904737.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8074..10158)
                     /gene="malQ"
                     /locus_tag="OQQ66_001775"
     CDS             complement(8074..10158)
                     /gene="malQ"
                     /locus_tag="OQQ66_001775"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1904738.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10168..12561)
                     /gene="malP"
                     /locus_tag="OQQ66_001776"
     CDS             complement(10168..12561)
                     /gene="malP"
                     /locus_tag="OQQ66_001776"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1904739.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13173..15878
                     /gene="malT"
                     /locus_tag="OQQ66_001777"
     CDS             13173..15878
                     /gene="malT"
                     /locus_tag="OQQ66_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCS1904740.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15921..16937)
                     /gene="rtcA"
                     /locus_tag="OQQ66_001778"
     CDS             complement(15921..16937)
                     /gene="rtcA"
                     /locus_tag="OQQ66_001778"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1904741.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIEIDGVTR
                     VSIE"
     gene            complement(16941..18167)
                     /gene="rtcB"
                     /locus_tag="OQQ66_001779"
     CDS             complement(16941..18167)
                     /gene="rtcB"
                     /locus_tag="OQQ66_001779"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1904742.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18356..19954
                     /gene="rtcR"
                     /locus_tag="OQQ66_001780"
     CDS             18356..19954
                     /gene="rtcR"
                     /locus_tag="OQQ66_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1904743.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSATGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19936..20694)
                     /locus_tag="OQQ66_001781"
     CDS             complement(19936..20694)
                     /locus_tag="OQQ66_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1904744.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20711..21541)
                     /gene="glpG"
                     /locus_tag="OQQ66_001782"
     CDS             complement(20711..21541)
                     /gene="glpG"
                     /locus_tag="OQQ66_001782"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1904745.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21586..21912)
                     /gene="glpE"
                     /locus_tag="OQQ66_001783"
     CDS             complement(21586..21912)
                     /gene="glpE"
                     /locus_tag="OQQ66_001783"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1904746.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22102..23607
                     /gene="glpD"
                     /locus_tag="OQQ66_001784"
     CDS             22102..23607
                     /gene="glpD"
                     /locus_tag="OQQ66_001784"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1904747.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23659..>24054)
                     /locus_tag="OQQ66_001785"
     CDS             complement(23659..>24054)
                     /locus_tag="OQQ66_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904748.1"
                     /translation="SVMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYI
                     SPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKN
                     RNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5653 a         6549 c         6080 g         5772 t
ORIGIN      
        1 cataatatat tccggcaata tttatcactt cattaacaac tgaaacctta attaaacatt
       61 agccagtccg ggtaattcac tattcgaatt atattttcgc tgcgatataa ccttgagcca
      121 catcaacatt gagtcagatt attattcaaa ccaacattcg cacacatttt aagtattgct
      181 gatagaaacc attctcatta tcattgtgtt attgattatt taatctctcc tttgttggca
      241 aatcatctgg tctcatgtcg ctgtcaaacg ccccatgagg tagttatcca gttaatgaga
      301 aacaagtagg cacctatgca atacactcca gatactgcgt ggaaaatcac tggcttttcc
      361 cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc ttggcatgtt acctggctcc
      421 tcttttaatg tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt
      481 gtgagcctgg tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt
      541 taatacggtg ataacaacaa tgaaaaaatt aaccataggc ttaattggta atccaaattc
      601 tggcaagaca accttattta accagctcac tggcgcacgt cagcgtgtag gtaactgggc
      661 tggcgttacc gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct
      721 ggtggaccta cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga
      781 gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac ctgctgatta acgtggtgga
      841 tgcgtctaac cttgagcgta acctgtacct gacgctacaa ctgctggaac tcggcattcc
      901 ctgcattgtg gcactgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat
      961 tgatgctctg tcggcgcgtc tgggctgtcc ggtgatcccg ctggtttcaa cccgtggtcg
     1021 cggtattgaa gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact
     1081 ggtgcattac gcacagccgc tgctcaacga agcagattca ctggcaaaag tgatgccttc
     1141 cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta
     1201 cagccgcgcc tacgccggtg aagcgtcgca gcatctggat gccgccctcg cccgtctgcg
     1261 taatgagatg gacgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc
     1321 catctgtgat gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt
     1381 agataaaatc gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct
     1441 gatgttcctg ctggctatca acatcggcgg ggcgttacag ccgctgtttg acgtcggctc
     1501 cgtggcgcta tttgtgcatg gtattcaatg gattggctac acgctccact tcccggactg
     1561 gctgactatc ttcctcgccc agggcctggg tggcggcatt aacaccgtgc tgccactggt
     1621 gccgcagatt ggcatgatgt acctgttcct ctccttcctt gaggactccg ggtatatggc
     1681 gcgtgcggcg tttgtgatgg accgtctgat gcaggcgctg ggcttgccgg ggaaatcctt
     1741 tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggtg cacgtacgct
     1801 tgatgcaccg cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc
     1861 gcgtctggct atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgcgctggc
     1921 ggtcttctcg ctgtatatgc tgggtattgt gatggcggtg ctgactggcc tgatgctcaa
     1981 gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg gagttgccgg tctatcatgt
     2041 accacacgtt aaaagcctga ttatccagac ctggcagcgt ctgaaaggct tcgttctgcg
     2101 tgctggtaaa gtgatcatca tcgtcagcat tttcctgagc gctttcaaca gcttctcgct
     2161 gagcgggaaa atcgtcgata acatcaacga ctcggcgctg gcgtccgtca gccgggtgat
     2221 caccccggtc ttcaagccaa ttggcgtgca tgaagataac tggcaggcaa cggttggcct
     2281 gtttacaggt gccatggcga aagaagtggt agtgggtacg ctcaacaccc tctacaccgc
     2341 agaaaatatt caggacgaag agttcaatcc ggcagagttt aacctcggtg aagagctgtt
     2401 cagtgcgata gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat
     2461 gaaccccatt gaagccagca aaggcaacgg cgaaatgggt accggggcga tgggcgtgat
     2521 ggatcagaaa ttcggtagcg cagcagcagc ttacagctac ctgattttcg tcctgctgta
     2581 tgtaccatgt atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg
     2641 cttctccatc ctgtgggggc tgaatatcgc ttactcgctg gcaacattgt tctatcaggt
     2701 cgccagctac agtcagcacc caacttacag cctggtgtgc attctggcgg ttatcctgtt
     2761 taacatcgtg gttatcggtc tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct
     2821 ggcaacccgc aagtcggtaa gcagttgctg cgcagccagc accaccggtg attgccatta
     2881 atggcttcac ttattcaggt gcgcgatttg ctggcgttac ggggccgtat ggaagcggcc
     2941 cagataagcc agacattgaa cactccacag ccaatgatta acgccatgct gcaacaactg
     3001 gaaagtatgg gcaaagccgt gcggattcag gaagaacctg acggctgcct ctctggcagt
     3061 tgtaaaagct gcccggaagg aaaagcctgt ctgcgcgagt ggtgggcgct tcgttaacct
     3121 tactccatcg cctgttttgg aaagcccggt atgcgtctgc atccgggctt ttttgcgtgc
     3181 ggctttccat aaaaatgcaa ctcttgcagc acggcgtaag ttcctttgaa agcatctcgc
     3241 agggatgaaa actcgctaat acacaggtgt ggagtggcgc gtagagtcgc ggcattcaaa
     3301 caacaggtga aggaacgcca tgagcaaaaa gcagagttcc accccacacg atgcgctgtt
     3361 caaactcttt ttacgccaac cggacacggc tcgtgatttt cttgcgtttc atttaccggc
     3421 acccattcac gcgctttgtg atatgaaaac cctcaagctg gagtcgagca gctttattga
     3481 tgacgatctg cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg aacaaggacc
     3541 aggatacatc tattgtctga ttgaacatca aagcacctca aacaaactga tcgcatttcg
     3601 catgatgcgt tacgctattg ccgcaatgca aaatcacctt gatgctggat acaaaacgtt
     3661 gccgatggtg gtgccattgt tgttttacca cggtattgaa agcccctatc cctattcgct
     3721 gtgttggctg gattgtttcg ccgatcccaa actggcaagg cagctttatg cctccgcatt
     3781 tccgctgatt gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc gacgtatggc
     3841 gctgctggag ttaattcaaa aacatattcg tcaacgcgac ctgatggggc tagtagagca
     3901 aatgggctgc ttattaagta gtggatacgc taatgacaga caaatcaaag ggctgtttaa
     3961 ttacatactg caaaccggcg acgcggtacg ttttaacgat tttatcgacg gcgttgccga
     4021 acgttcaccg aaacacaagg agagtttaat gacaattgcg gaaagattgc ggcaggaggg
     4081 ggaacaatcc aaagccctgc atatagccaa aataatgctt gaatccggag tccctcttac
     4141 agacatcatg cgctttaccg gggtgtcaga agaagagttg gctgcggcga gccagtaaat
     4201 ttctgtctcg ccatttcaaa agttgcctac accctctgct tcaacgccac cagcaggtga
     4261 caaaactcga ccggatgcga aataaacggc gcatgggccg ctttggcgaa gatatatgat
     4321 ttgctgcgag gccagagttt atccagcatc ggcaccactt tgcgcggcac cagaccatcg
     4381 agatagccat acaatcgcaa aaacggcatg ggcacgtttt gcagcggctg acggagatca
     4441 accgttttca ggatttccag cccgccatta agcacgtcaa cctccggcat cggtaacgcc
     4501 agaacggttt tcttcaacgc ccgcgcatcc tggcgcgccg tttcagtccc catggtttgt
     4561 aacgccagga accgctccac tgtacgctga aaatcatcac tgagttgctg ctgaaatccc
     4621 gccagcacgt ccggttttat ccccggccaa tcgtcacgag cactaaaaca aggtgacgac
     4681 gccacggtga ccagcgcctg aacacgctcg ggatgggtta acgcaatctg gcttgccacc
     4741 agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgttg cagcacggct
     4801 tcggccatat cagcaagtga cagcgcacca aatccctggc tgcgcccgaa gccgggcaga
     4861 tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg ccacacttcg
     4921 gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc tttggtctgc
     4981 caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga acatatgcta
     5041 acagtaccgg gattatgctg gctatgccga atgccgctgg cgttaggtca ttgggggatt
     5101 tgttcggtct gctcacgcgc cgcccgtaca gataaaacgt tatgcccaca atgtggatta
     5161 cccgccacac actcacatct tccctgcggt cgctgcctgc aaaaaccacc gccctggcaa
     5221 agactggtca cggttgccga ctatgcgccg ccgttaagtc cgcttatcca ccagcttaaa
     5281 ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta
     5341 cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca tcatcagcgt tccgttatgg
     5401 cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca gccgttatca
     5461 cgctggttgc actgccgatg ggatagcgaa gccgtcacac gaacacgggc cactgcgacc
     5521 cagcattttc tcagtgcccg gctacgcaag cgcaacctga aaaatgcctt tcgtcttgaa
     5581 ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac cggaagtacc
     5641 gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc
     5701 ctttgtcgaa ccttgtagag cctcgatgat aggcgtatta taaccaacta aaatagtcaa
     5761 ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact ttgccaaact
     5821 gctggcaaac caggaagaag ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc
     5881 taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca ccgacactgc
     5941 cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgctcctt acctggaaga
     6001 tgcagagatc gattttgtta ccgaccagtt gggttctcag ctaacactga aagcgcctaa
     6061 cgccaaaatg cgtaaagtgg cagacgatgc accactgatg gagcgcgtgg agtatatgct
     6121 gcagtcgcag atcaacccac agcttgctgg tcacggtggc cgcgtttcac tgatggaaat
     6181 caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt gttccatggt
     6241 cgatgtgacg ctgaaagaag ggatcgagaa gcagctgcta aacgaattcc cggagctgaa
     6301 aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact aagttatccc
     6361 ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac gttgaacatg
     6421 tgccagacgt tggcgcagca ttgctttggc aaatccctcc cttcgtttct ccgcgataat
     6481 atgaccaacc tctcataatt taaatttact ccgctctggt gattctcaaa cgccagatgt
     6541 taccggtatc attcacatgg gtaccaaaca tactcctgac atctgactac aataattagt
     6601 tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga taattagagt
     6661 ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa tcatgccatt
     6721 agtcattgtt gctatcggtg taatcttgtt gttgctcctg atgatccgtt tcaaaatgaa
     6781 cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag ggatgccgct
     6841 ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta gccttgccct
     6901 gatcatgggt tttggcgcga tgctgggcaa aatgctggca gactgcggtg gcgcacaacg
     6961 tatcgccacc accctgattg ccaaatttgg taaaaaacac atccagtggg cggtggtact
     7021 gaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggctttgtgc tgatgctgcc
     7081 gctggtgttt accatcgcgg cttctgcgaa tattccactg ctgtatgttg gtgtaccaat
     7141 ggcagctgca ctgtctgtga cccacggctt cctgccaccg catccgggtc cgactgcgat
     7201 tgccaccatt ttcaatgccg acatgggtaa aaccctgctg tacggtacta ttctggcaat
     7261 cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta tagataagcc
     7321 gatccctgaa ggtctgtaca gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg
     7381 cgtcagcgtc tggacctctc tggtgccggt agtgctgatg gcgatgcgtg cgattgccga
     7441 aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg gtgacccggt
     7501 aatggctacg ctgattgccg tgctgattgc gatgttcacc tttggtctga accgtggtcg
     7561 ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg cgatgatgct
     7621 gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa
     7681 atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg cctggtcgat
     7741 tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg
     7801 tatcgcggca ccgctgattg caacgacggg tgttagcccg gagctgatgg ttattgcggt
     7861 gggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc tgttcaaaga
     7921 gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg aaaccattat
     7981 ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat aacactgact
     8041 gccggatgca tcgcatccgg catcaaccgc actctactgc ttcttcgctg cagctctgcg
     8101 ccgtctgtcc aaatccttca gcaacttatt cacgccatca tcggcaaaca tcgtctcaag
     8161 cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag gaatattcac
     8221 cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac tgttactgtc
     8281 ggcaatgtag cgctgcaaac cacgattaag cgtcggcgtc atcgacatca acgatgcctt
     8341 atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca gcccttgctt
     8401 cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat acagccccag
     8461 ggttttgccc aacgttagat ccccgctctc ccagtaaccg cgcagcgtag gcaggtcatg
     8521 tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga acgtcttctc
     8581 gtggtcgttt tcgaaataga gcactttgta ggagtacaca ccgctgctgc gcagcttacc
     8641 gacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc gatgacgttt
     8701 actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat acgcgccctg
     8761 atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca catggtcaat
     8821 tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg gttcataggc
     8881 acgcgcggtg atgatatgcg ggtccattgg cggtaatccc cagttctgcc ccaacgggcc
     8941 gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa tatagttcac ggtcacacca
     9001 ggtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc caatcggcat
     9061 ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca gccactgcaa
     9121 ccagagataa aaatcgacgt catcacgatg ttcttcgcag aactgacgca cttctggtga
     9181 atccacgttc tgatacatct ctggccatgc aggccagccc cagcgcattt cgtcctcttt
     9241 cacttgctgg gcatgtagcg catcaaaggc tgcctgccag aacaggctgt cgccctgctc
     9301 tgcaacaaac tggcgaaacg cggtcatctg ctcatcatca cgttgcgcga aacctttcca
     9361 cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcga cccagtcggc
     9421 atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc
     9481 ttcgctaaga tggaaatctt caacggcgtt aacgtcgata taaatcacat tcagccaacg
     9541 gcgagaagac ggactgtatg ggctggcact ctccggattt gccggataga gcgcatgaat
     9601 cgggttcagg ccgatgaacg acccgccacg ttttgccaca tccaccagca tcgctttgag
     9661 atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa gctgaacgca
     9721 ggcaccccac agcttttgtt tattcagcaa cgcctgcggt tcgtaacagc gtttcggggc
     9781 gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg agtgtcagcg tgtgataacc
     9841 ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttacatggc ctttgtactg
     9901 tgttccttct tcggtggtca gcagccagct atattcgccg ctgccctcca ccaccatcgg
     9961 catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg ccactttcgt
    10021 ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg
    10081 cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg cggcattatc
    10141 cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac gagcctgata
    10201 atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga ggatcgccgc
    10261 gcgagtccag gcctcttggt cgcggtacag cacatccacc tgcttttgtg cctcaacata
    10321 ggctgcgaaa tccgccatca ccagatacgg atcgccgcct tgtttgccga tactgtgcag
    10381 catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca gctctttcaa
    10441 tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc ctttggccag
    10501 aattgccttc acttgttcca cggtatgacc aaaaataaag atattttctt caccgacttt
    10561 ctcggcgatt tcaacgttcg caccatccag cgtaccgaca gtaagcgcac cattgagcgc
    10621 cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt gttcggagat
    10681 atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatcctgca ggaacaccac
    10741 cttcaactta tcgccaacca gtggatcgtt gttgatcacg tcagccactt tgttgatcgc
    10801 aaagataata ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga agaggaagac
    10861 gcgcggtacg cgatcagcct gcgggttttc acgaatttct ttgtacaacg ccagaatatg
    10921 cagcagattc aggtgctggc gtttgtactc atgcagacgt ttaatctgaa tatcgaaaat
    10981 cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca gacggacttt
    11041 attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag cgaatttttc
    11101 cagattgatc agctgatcga gatcgttagc ccactctttt tgcagtaatt tatccaacag
    11161 agccgccagt gcagggttgc actgtttgat ccagcgacgt ggggtaatac cgttggtgac
    11221 gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt tcaccaccag
    11281 atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac acaggttcgc
    11341 catatgcact tgtttatcgt gcaccaccgc cagtttggcc cacacttttt catcgcccgg
    11401 ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa tctgcatatg
    11461 gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt ctggcatcag
    11521 ggtatggttg gtgtaagcga aagttttgct ggtaatggcc caggcgtcat cccagctcat
    11581 ctggtgctca tcgatcagca cgcgcagcag ttctggaatc gcgatagttg ggtgggtatc
    11641 gttcagctga ataacttcat aatccgccag ttcgtgcagt ttacgccccg ccagatgatg
    11701 gcgacgcaaa atatccgcta ccgaacaggc acactggaag tactgctgca tcaagcgcag
    11761 ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt tttccgcatt
    11821 gatgccctgc tgttcggcac gcaggaaatc accgtcgtta aatttagtca gatcaaacgg
    11881 atgcgcgtgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac gatagccgac
    11941 aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt
    12001 cgtcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc ggaaccacgg
    12061 gtagttactg cgatgccagt catccggcgc ttcaacctgt ttgccatcga caaaagactg
    12121 gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac cgatagttgc
    12181 cattgagtcg aggaagcacg ccgccagacg tcccagacca ccgttaccca gcgccgggtc
    12241 gatctcttct tccagcaggt ccgtcagatt gatgtcataa gccttcaacg aatcctgtac
    12301 ttcctgatac cagccgagat tcaacaggtt gttgcccgtc aggcgaccaa tcaaaaactc
    12361 cattgagatg tagttaacat gtcgctgatt cgccaccggc ttggcgaatg gctgagcacg
    12421 cagcatttcg gccagtgctt cactcactgc tagccaccac tggcgaggag tcatttcagc
    12481 cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa attgcttatc
    12541 gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac acaacgttat
    12601 cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta ggcagggagg
    12661 agttgcgggg atgagcaagg aaatgtgatc tcaaccactt aaagctagtg caaaccacag
    12721 gattagcagc aaatcaatgc aatacagcgc agaaaatctg tatctaagtg caaaaaatgg
    12781 ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag gagtgaaaca
    12841 gtttcacaag taaaatatcc agtgtgctcc atctcattct taatagattt attaagatca
    12901 tctttttaga tggcactttc atcaggaatg aagaagaaac ccttgcttaa atgaatctga
    12961 tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat
    13021 aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc
    13081 attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata
    13141 tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc
    13201 cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga
    13261 acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc accctcattt
    13321 cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa ggtgataacc
    13381 agcaagagcg tttcgccagc tatctcattg ccaccgtgca gcaggcaacc aacggtcact
    13441 gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg
    13501 cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact
    13561 atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac
    13621 cagaaaatct caccctggtg gtgttgtcac gcaaccttcc gcaactgggc attgccaatc
    13681 tgcgtgttcg tgaccaactg ctggaaattg gcagtcagca actggcattt acccatcagg
    13741 aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca gaaagcagtc
    13801 ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc ctctccgccc
    13861 ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc aatgccagcc
    13921 atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca acgcgccatt
    13981 ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc gctcatcacc cgtgtgaccg
    14041 gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg tttttacagc
    14101 ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac ttcctgcgcc
    14161 agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa
    14221 gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg gcaggcgatg
    14281 cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac catagcgaac
    14341 tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc
    14401 agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc gaagttaaca
    14461 ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcacgcag
    14521 aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatccggat gaagcggaac
    14581 ggctggcaaa actggcactg gaagagctgc caccgggctg gttctatagc cgcattgtgg
    14641 caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc
    14701 taatgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac gccttatgga
    14761 gtttaatcca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa
    14821 cacaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg ccgatgcatg
    14881 agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg
    14941 aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag caacagcttc
    15001 agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta
    15061 gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct
    15121 ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg
    15181 ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc
    15241 aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg
    15301 ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta
    15361 acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg
    15421 tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg
    15481 aaggcgaagc gatggcgcaa caactgcgcc agctgattca actcaatacg ctgccggaac
    15541 tggaacagca tcgcgcgcag cgtattctgc gagaaatcaa tcaacatcat cggcataaat
    15601 tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac
    15661 tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg atctactccg
    15721 gttacagcaa tgagcaaatt gcgggcgagc tggaagtggc ggcaaccacc atcaagacgc
    15781 atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggccgcg gtacaacacg
    15841 cgcagaaact gctgaagatg atgggatatg gggtgtgagt ttagccggat aatgcattgg
    15901 atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc tatttctatc
    15961 aaaccaaacc gcaccggcaa gaaacgctcc accacggcga tattggtcag caaatggcat
    16021 gaaggatggg cgaccgtaaa ttctcccgcg cccgccagcg ccatcggtag caccagctgg
    16081 tcggcgagat attcccccac tgccgccggg cttgccaggt agcgtttcac ctctttcact
    16141 aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacgaa aaagcgttca
    16201 gtgatatttt cactttcgac ttccagcgag acggtattac ccggcccctg gtcgcgcggc
    16261 aggttatgaa tattctgttc atgcagggaa aaactccccg ccagtgtagc gatttcacgc
    16321 tcagcaacat ggcgcggcac accagctaat agaacttctc cacgcatctg cacaatgttc
    16381 ccgcgctcgc caagttgcaa ggtgttaaac gatgccacgg gcgagacttc cgttgccacc
    16441 acgccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg atgaattcct
    16501 attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg cgccgacggg
    16561 ttatcggtgc ctccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc
    16621 agcaccgttt gcagcaccag cgtacagctt ccggcactac cgatagcaaa gcggtactcg
    16681 ccgccgcgca cggcgccagg ccggaagacc agacgctgcg accccagctc cgcgccttcc
    16741 accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgctg gcgcaacagc
    16801 cccggtttcg cccgcccggc acgaatgctg gtgatggtaa atggctggcc ggttatcatc
    16861 gacaggctca gcgccgagcg cagaatctgc ccgccacctt cgccctgtgc gccatccagc
    16921 gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag ggtatagata
    16981 acttccacca gatcgctttg tgccgtcatc accgcatcaa tatctttata cgccatcggg
    17041 atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtggc acgaatttga
    17101 tcttccacgc tgaacagttt tttcgcttta gttcggctca ttacccgccc ggcaccgtgg
    17161 ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct ttttgctccc
    17221 atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcgcc tttacgcgtc
    17281 acgtagatct cttcaccaaa gtgctgttct ttttgcacat agttgtggtg acagttgatc
    17341 tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct ctgcaatgcc
    17401 gttaccacgt tttccatcat cacatcgcgg ttaaggctgg caaaaagctg cgcccaggcc
    17461 acggctttca ggtaatcatc aaagtattcc gtaccttcca taaagtacgc cagatcacgc
    17521 gacggcaacg tctcaagcgt ttcctgcatc tctttttgtg ccagatcgat aaagtaagtc
    17581 ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca taatccacac ctggtccgac
    17641 tcatcaaggc agattttaat aaagtggtta ccggttccca gcgttcccag gtgtttatag
    17701 ttattggtat tcaggaaacg gggatatttt tgcgttaacc actgataacc ggcttcaagc
    17761 tcagcccatt tcgcatcgac gttgacaggc gggttttccc atgcgccttt atcacgttta
    17821 caacgcccgg tagtacgccc gtgcggcacg gccgtttcaa tcgcctgacg cagctctgcc
    17881 aggttttcag gcaggtcttc cgccgttaac gcggtacgca gcgcgttcat tccacagcca
    17941 atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac gctaccaatg
    18001 gtggaatctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa aataaacggc
    18061 atcttcgccg tattaataag ttgctgacgc gcatcggctt ctaccggcac gcctttggtc
    18121 cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt gtttttctct
    18181 tttcgttgtt tgctgtcctg ataattgcaa ccgtcgtgcc agaaaattaa ataagtaaat
    18241 taatttttta attcattgtt tttaaaaaga ttatatcttt atgtccgtaa ccggagattt
    18301 cccgcaaagc cgatttaccg ataatgaaat atcgtctttt ataaggatat ctaagatgcg
    18361 taaaacagta gcttttggct ttgtcggaac ggtgctggat tatgccgggc gcggcagtca
    18421 gcgctggtca aaatggcatc cgacactctg tttatgccag caagaatcgt tggtcatcga
    18481 tcgactggaa ttattgcatg acgcccgctc gtgctcgcta tttgaaacgc ttaaacgcga
    18541 tatcgccagc gtttcgccag aaacagaagt ggtgagcatt gagattgaac tgcataaccc
    18601 gtgggatttc gaagaggttt acgcctgcct gcatgatttc gcccgtggtt acaagtttca
    18661 gccagaaaaa gaagactatt taattcacat caccaccggc acccacgtcg cgcagatttg
    18721 ctggtttctg ctggcggaag cgcgttatct acccgcccgg ttgatacaaa cttcaccacc
    18781 gcgcaaaaaa gaacaaccgc gcggtgccgg tgaagtgacg attatcgatc tcgatctaag
    18841 ccgttataac gccatcgcca gccgctttgc cgaggaacgc cagcaaacgc ttgattttct
    18901 taagtccggc attgccacgc gtaaccccca cttcaaccgc atgattgagc agattgaaaa
    18961 agtggcgatt aaatcccgcg caccgatcct gcttaacggc ccaaccggtg cgggaaagtc
    19021 atttctggcg cgacgtatat ttgaattaaa acaggcgcgg catcagttta gcggcgcgtt
    19081 tgtggaagtg aactgcgcca ccctgcgcgg cgataccgcc atgtcgacgc tgtttggtca
    19141 tgtgaaaggc gcgtttaccg gggcgcggga atctcgtgaa ggtttattac gcagcgccaa
    19201 cggcggaatg ttgttttttg atgagattgg cgaactgggc gcagacgaac aggcaatgct
    19261 gctaaaagcc attgaagaga aaacctttta cccgtttggc agcgatcgcc aggtgagcag
    19321 tgattttcag cttatcgccg gaacggtgcg cgatttgcgc cagctggttg ccgaaggcaa
    19381 atttcgcgaa gatctgtacg cgcggatcaa tctctggacc ttcaccctgc cgggtctacg
    19441 ccagcgccag gaagatattg aaccgaacct tgattatgaa gtggagcgcc acgccacact
    19501 cactggcgac agcgtgcgtt ttaacaccga agcgcggcgc gcctggttgg cttttgcgac
    19561 ctctccccag gcaacatggc gcggtaactt tcgcgaactt tctgccagcg tcacgcggat
    19621 ggccaccttt gccactagcg gacgcatcac tctggacgtg gttgaagatg agataaaccg
    19681 tctgcgctat aactggcagg agagccgccc ctccgcgctt acggcgttgc tgggcgcaga
    19741 ggcagaaaac atcgatctct tcgaccgtat gcaactggaa cacgttatcg ctatctgccg
    19801 ccaggcaaag tcgctttctg caaccggacg acagcttttt gatgtttcgc gccagggaaa
    19861 agccagcgtc aatgacgcgg atcggctacg caaatacctg gcgcgttttg gtctgacgtg
    19921 ggaagccgtg caggatcagc acagctccag ttgaatatga tggtccgtca gcacctgcat
    19981 tacgcttact ggcggcgggg cgtcggtgta gacggcatct accatgctga tgctgcccat
    20041 attgaccatc gcgttacggc caaatttcga gtgatcgaca accagcataa cgtggcgcga
    20101 gttctcaata atggcgcgct tggtgcgaac ttcgtggtaa tcgaactcca gcagcgagcc
    20161 gtcgctatcg atgccgctta tccccagaat gccgaaatca aggcggaact gggagataaa
    20221 atcgagcgtc gcttcgccaa tgatcccgcc atcgcggctg cgtaattcgc caccagcgag
    20281 aataatgcgg aaatcttctt ttaccatcaa cgtgttagca acattgagat tgttggtgac
    20341 aatacgcaaa ttgctgtgat taagcagcgc gtgcgccacc gcttccggcg tggtgccgat
    20401 atcgataaac agtgtcgagc cgttggggat ttgctccgcc actttgcggg caatcctctc
    20461 tttttcttcg gtctgggtgg ctttgcgatc gtgccacggc gtgttaaccg aactggaagg
    20521 cagcgccgca ccgccatggt gacgcaggat caggttttgc tccgccagct cattgaggtc
    20581 gcggcgaata gtctgcgggc tgacggagaa atgctctacc agctcttcag tactgacata
    20641 accctgctgt ttaaccagtt cgataatacc gttgtgacgt tgtgtttgtt tcatttataa
    20701 atccctggaa ttattttcgt tttcgcgcat tgagcgaatc aacaaaagcc atcgctaaac
    20761 ccacggctaa cccggcgatg tgtgctccgt tcgccatcga catcccaaac aaatcaaacc
    20821 atccggcgat aatccagatc agcgcaaaga taattaaccc acgttgcagg taaatgccac
    20881 tttgcggatc gcgctcacca cgtagccaga cgtagcccat cagcgcatac accacgccag
    20941 aaagcccgcc aaaccacggc ccgctgaatt tttgctgcac atagccgctt aacagggcac
    21001 taatgagagt aataacaatt agcttaccgc taccgaggcg tttttccacc gcaccgccga
    21061 gataccacca ccagagcagg ttaaagagga tatgcatcag cgagaagtgc attaacgcgt
    21121 gggtgaagta acgccagaac tcaaatttca gtgttggatc gaatggccag gccagccata
    21181 acatcacttc ctgatcgccg agaatttgca tggcgataaa caccaccacg caggcgatca
    21241 tcaccaccca ggttaccgga cctgcgcgtt cacgcaaggc agcaaagaaa ggataacggc
    21301 gataatgcag accactgccg gtatggcccg cctgccagct ggccgccaga taacgcggat
    21361 ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgcac gcgctcggcc tgggactcat
    21421 ccgccagcca gacatcgctt tggttatgtt gttgaatcgt aaggataaca ccctgcgtcg
    21481 ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc aaaagaggta atcatcaaca
    21541 tcgttgctgt cgcttattcc acacaaaagg ggacagtata aagcgttacg cgccgtacgc
    21601 cacctctgcg ggaaactgac gctgccaggc ttcaaaaccg ccgtcaatgc tatagaccac
    21661 atcgtagccc tgttgcagca gatactgcgc cgcgcctttg ctgctattgc cgtgataaca
    21721 catcaccatc accggagtgt caaagtcgtt atcacgcata aacgcgccca gcgtgtcgtt
    21781 ggttaaatgg aaagcctgca ccgcatgtcc cattgcgaaa ctctgtggat cgcgaatatc
    21841 gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg tcggcaacgt taatacattc
    21901 gaacagatcc atgcgtctct ctttctttac aaacaagtgg gcaaatttac cgcacagttt
    21961 acgtcgaagc ggcagataaa cgccataatg ttatacatat cactctaaaa tgttttttca
    22021 atgttaccta aagcgcgatt ctttgctaat atgttcgata acgaacattt atgagcttta
    22081 acgaaagtga atgagggcag catggaaacc aaagatctga ttgtgatagg tggcggcatc
    22141 aatggtgctg gtatcgcggc agacgccgct ggacgcggtt tatccgtgct gatgctggag
    22201 gcgcaggatc tcgcttgcgc gacctcttcc gccagttcaa aactcattca cggtggcctg
    22261 cgctaccttg agcactatga attccgcctg gtcagcgagg cgctggctga acgtgaagtg
    22321 ctgctgaaaa tggctccgca tatcgccttc ccaatgcgtt ttcgcctgcc acatcgtccg
    22381 catctgcgcc cggcgtggat gattcggatt ggtctgttta tgtacgatca tctgggtaaa
    22441 cgcaccagcc tgccgggatc aaccggtttg cgttttggcg caaactcagt gttaaaaccg
    22501 gaaattaagc gcggattcga atattctgac tgttgggtag acgacgcccg tctggtactc
    22561 gccaacgccc agatggtggt gcgtaaaggc ggcgaagtgc ttactcggac tcgcgccacc
    22621 tctgctcgcc gcgaaaacgg cctgtggatt gtggaagcgg aagatatcga taccggcaaa
    22681 aaatatagct ggcaagcgcg cggcctggtt aacgccaccg gcccgtgggt gaaacagttc
    22741 ttcgacgagg ggatgcatct gccttcgcct tatggcattc gcctgatcaa aggcagccat
    22801 attgtggtgc cgcgtgtgca tacccagaag caggcctaca ttctgcaaaa cgaagataaa
    22861 cgtattgtgt tcgtgatccc gtggatggac gaattttcca tcatcggcac caccgacgta
    22921 gagtataaag gcgatccgaa agcggtgaag attgaagaga gtgaaatcaa ctatctgctg
    22981 aaagtgtata acgcgcactt taaaaaacag cttagccgtg acgatatcgt ctggacctac
    23041 tccggtgtgc gtccgctgtg tgatgatgag tccgactcgc cgcaggctat tacccgtgat
    23101 tacacccttg atatccatga tgaaaacggc aaagcaccgc tgctgtcggt attcggcggt
    23161 aagctgacca cctaccgaaa actggcagaa catgcgctgg aaaaactgac gccgtattat
    23221 cagggtattg gcccggcgtg gacgaaagag agcgtgctgc cgggtggtgc cattgaaggc
    23281 gaccgcgacg attacgcagc tcgtctgcgc cgtcgctatc cattcctgac tgaatcgctg
    23341 gcgcgtcatt acgcacgcac ttacggcagc aacagcgagc tgctgctcgg caatgcgggg
    23401 gcgataagcg acctcgggga ggatttcggt catgagttct acgaagcgga actgaaatac
    23461 ctggtggatc acgaatgggt ccgccgcacc gacgatgccc tttggcgtcg cacaaaacaa
    23521 ggcatgtggc tgaatgcgga tcaacaatct cgtgtgagtc agtggctggt ggaatatacg
    23581 cagcagaagt tgtcgctggc gtcgtaagga tgatgcccgg ttgaagtgca accgggctat
    23641 ttggattcct tagactattc attgtaaata attgaataac ggcccctgag ctaaaatcgc
    23701 caaacttaag cacgttaccc aatcgcccca cttcttcctc tggtttctat tttttctgca
    23761 ccaatggcaa taatatccca atgcaaaacc aagctgtgca ctaatcgggc cgacaaaatc
    23821 agtccaaggc tgcggccaaa aaggggaaaa attcagggga ggccagacaa acgtcagaaa
    23881 gaaatttcgg atattttcca gcaacgctga tttctctgga gaaatataaa gaatgaaatt
    23941 cgagagaatt aaccagaaat agaccggcga aaagaccagc cagcaatgca gtctgttttt
    24001 taattcagga ggcgttctgc gggagaattg ccaaccgatg ccagccatta cact
//
LOCUS       DAJVTY010000050        23023 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVTY010000050 DAJVTY010000000
VERSION     DAJVTY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23023
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.050"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..555)
                     /locus_tag="OQQ66_001786"
     CDS             complement(<1..555)
                     /locus_tag="OQQ66_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1904749.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLN"
     gene            complement(774..1751)
                     /gene="epmA"
                     /locus_tag="OQQ66_001787"
     CDS             complement(774..1751)
                     /gene="epmA"
                     /locus_tag="OQQ66_001787"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1904750.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLNLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2076..3884
                     /gene="frdA"
                     /locus_tag="OQQ66_001788"
     CDS             2076..3884
                     /gene="frdA"
                     /locus_tag="OQQ66_001788"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1904751.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3877..4611
                     /gene="frdB"
                     /locus_tag="OQQ66_001789"
     CDS             3877..4611
                     /gene="frdB"
                     /locus_tag="OQQ66_001789"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1904752.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4622..5017
                     /gene="frdC"
                     /locus_tag="OQQ66_001790"
     CDS             4622..5017
                     /gene="frdC"
                     /locus_tag="OQQ66_001790"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1904753.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5028..5387
                     /gene="frdD"
                     /locus_tag="OQQ66_001791"
     CDS             5028..5387
                     /gene="frdD"
                     /locus_tag="OQQ66_001791"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1904754.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5450..6583
                     /locus_tag="OQQ66_001792"
     CDS             5450..6583
                     /locus_tag="OQQ66_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1904755.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6672..7205
                     /gene="blc"
                     /locus_tag="OQQ66_001793"
     CDS             6672..7205
                     /gene="blc"
                     /locus_tag="OQQ66_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1904756.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGLFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7202..7519)
                     /gene="sugE"
                     /locus_tag="OQQ66_001794"
     CDS             complement(7202..7519)
                     /gene="sugE"
                     /locus_tag="OQQ66_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1904757.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7701..7847)
                     /gene="ecnB"
                     /locus_tag="OQQ66_001795"
     CDS             complement(7701..7847)
                     /gene="ecnB"
                     /locus_tag="OQQ66_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1904758.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7958..8083)
                     /gene="ecnA"
                     /locus_tag="OQQ66_001796"
     CDS             complement(7958..8083)
                     /gene="ecnA"
                     /locus_tag="OQQ66_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1904759.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8135..8701)
                     /gene="efp"
                     /locus_tag="OQQ66_001797"
     CDS             complement(8135..8701)
                     /gene="efp"
                     /locus_tag="OQQ66_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1904760.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8743..9792
                     /gene="epmB"
                     /locus_tag="OQQ66_001798"
     CDS             8743..9792
                     /gene="epmB"
                     /locus_tag="OQQ66_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1904761.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10166..11035
                     /locus_tag="OQQ66_001799"
     CDS             10166..11035
                     /locus_tag="OQQ66_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1904762.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11238..11591)
                     /locus_tag="OQQ66_001800"
     CDS             complement(11238..11591)
                     /locus_tag="OQQ66_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1904763.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11729..13375)
                     /gene="groL"
                     /locus_tag="OQQ66_001801"
     CDS             complement(11729..13375)
                     /gene="groL"
                     /locus_tag="OQQ66_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1904764.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13419..13712)
                     /locus_tag="OQQ66_001802"
     CDS             complement(13419..13712)
                     /locus_tag="OQQ66_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1904765.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13988..15244
                     /gene="yjeH"
                     /locus_tag="OQQ66_001803"
     CDS             13988..15244
                     /gene="yjeH"
                     /locus_tag="OQQ66_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1904766.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15260..15736)
                     /gene="fxsA"
                     /locus_tag="OQQ66_001804"
     CDS             complement(15260..15736)
                     /gene="fxsA"
                     /locus_tag="OQQ66_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1904767.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16073..17509
                     /gene="aspA"
                     /locus_tag="OQQ66_001805"
     CDS             16073..17509
                     /gene="aspA"
                     /locus_tag="OQQ66_001805"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1904768.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17627..18928
                     /gene="dcuA"
                     /locus_tag="OQQ66_001806"
     CDS             17627..18928
                     /gene="dcuA"
                     /locus_tag="OQQ66_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1904769.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19044..19382
                     /gene="cutA"
                     /locus_tag="OQQ66_001807"
     CDS             19044..19382
                     /gene="cutA"
                     /locus_tag="OQQ66_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1904770.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19358..21055
                     /gene="dsbD"
                     /locus_tag="OQQ66_001808"
     CDS             19358..21055
                     /gene="dsbD"
                     /locus_tag="OQQ66_001808"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1904771.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21092..21667
                     /locus_tag="OQQ66_001809"
     CDS             21092..21667
                     /locus_tag="OQQ66_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1904772.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21774..21849
                     /locus_tag="OQQ66_001810"
     tRNA            21774..21849
                     /locus_tag="OQQ66_001810"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21807..21809,aa:Phe,seq:gaa)
     gene            22047..>23023
                     /locus_tag="OQQ66_001811"
     CDS             22047..>23023
                     /locus_tag="OQQ66_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1904773.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5737 a         5937 c         5699 g         5650 t
ORIGIN      
        1 atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac acattactgc
       61 aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag caacgacggt
      121 gaccagaatc atccatgcta ctgccagcag accaaccacc tgcgtaggct ccagtccggc
      181 aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg gtacccaaac
      241 tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa acgtaccaat
      301 gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac cttcttcttt
      361 gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga ataacaatgc
      421 agaaaatata taaaagggaa tcgcactata acccattaag taataagccg atgggctatt
      481 ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac tcatcttttt
      541 tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg tgcccactga
      601 actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg atacccggat
      661 gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa catatgctac
      721 agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca gaattatgcc
      781 cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc caacatcacc
      841 agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt caaggcttca
      901 atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt acggttatct
      961 tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc attcgccagc
     1021 tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc ggtactgatt
     1081 tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt ttctttgcca
     1141 atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg gtcttcttcg
     1201 gtatcagcaa cgttactcaa attcagtttc gctgcgactt cccgcagttg cgttttgtcg
     1261 gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga aaggctttct
     1321 gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat caaccggtac
     1381 atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg ataacgcccc
     1441 atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc ggcaaccagc
     1501 aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc ctgcgaatgc
     1561 ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac cgtcgcctgg
     1621 ctcatacaag gcgtctccac ctccagcact ccacgatcag caaagaaacg acggatctcc
     1681 gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg ccaggatgcc
     1741 gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac tcgcaacgcg
     1801 acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta aaaaaagcac
     1861 gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc ctcttgcgca
     1921 ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact gttgtaccta
     1981 taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac cctgaagtac
     2041 ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa gccgatcttg
     2101 ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg caggcaaatc
     2161 caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat accgttgctg
     2221 cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat cactttcacg
     2281 atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat ttcgtccacc
     2341 actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc cgtcgcccgg
     2401 atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc tggttcgccg
     2461 ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg caattcccgc
     2521 agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat ggtcatgttc
     2581 gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt gctaacgcgg
     2641 tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac ggcggcatcg
     2701 ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt gacatggaat
     2761 tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc gaaggctgcc
     2821 gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa gattacggca
     2881 tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg ggtccacgcg
     2941 acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc tccacgccgc
     3001 gtggcgatgt ggtttatctc gacttgcgtc acctcggcga gaaaaaactg catgaacgtc
     3061 tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt aaagaaccga
     3121 ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat cagaactgtg
     3181 aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt ctgcacggtg
     3241 caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt atggccggtg
     3301 aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagaggca attgaagcgc
     3361 aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc ggcgaaaact
     3421 gggcgaagat ccgcgacgaa atgggcctag caatggaaga aggttgcggt atctaccgta
     3481 cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa cgcttcaagc
     3541 gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac accattgaac
     3601 tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca cgtaaagagt
     3661 cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac gtcaacttcc
     3721 tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag tacagcgacg
     3781 tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg gatgcagccg
     3841 ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa aaacctgaaa
     3901 attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc attctatgaa
     3961 gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa agacaacctg
     4021 gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg
     4081 atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta caccgacggt
     4141 atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt cgatatgacc
     4201 cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg
     4261 gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca gttctccggt
     4321 tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc
     4381 atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg cgaccacggt
     4441 aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg tactttcgtg
     4501 ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat tcagcagggc
     4561 aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta aggagtgcaa
     4621 catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt
     4681 gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg tgtggttcag
     4741 cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggacgcct gggcgggatt
     4801 cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc tggcggcagc
     4861 tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa
     4921 agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa ctgtggttgc
     4981 caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg attaatccaa
     5041 atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt ggtatgtgga
     5101 gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca ctggggctgt
     5161 ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc
     5221 gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac cgtatgcacc
     5281 acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc tacggtctgg
     5341 ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt atcgccaatg
     5401 taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta tgttcaaaac
     5461 gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat
     5521 caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga tccccggtat
     5581 ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct atgcggacat
     5641 cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac
     5701 atttacgggc gtgcttggtg gcgatgctat tgctcgaggg gaaatcaagt taagcgatcc
     5761 cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca cactattaca
     5821 tcttgcgacc tacaccgctg gcggcctgcc attgcagatg ccggatgaag tgaaatcctc
     5881 aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag gaacacaacg
     5941 tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt
     6001 gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca accatacgtg
     6061 gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc
     6121 agttcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt cgaccattga
     6181 agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg agaaaacgct
     6241 tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg
     6301 cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta acggcagtga
     6361 caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt
     6421 acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt
     6481 tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc
     6541 gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca tcgggtccga
     6601 attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta
     6661 aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg
     6721 ttgcctgcag ttctcctacg ccgccgcgtg gcgtgacagt agtgaataat ttcgacgcta
     6781 aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac
     6841 tggaaaaagt caccgcaaca tacagtctgc gtgatggcgg cggcctgaat gtcatcaata
     6901 aaggctataa ccctgacaga gaaatgtggc agcagagtga agggaaagcg tactttaccg
     6961 gcgcaccaac acgcgctgcg ctgaaagtgt cattctttgg tcttttctat ggcggttata
     7021 acgtcattgc acttgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact
     7081 acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg
     7141 cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta
     7201 gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca
     7261 ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatagcc gcgccgactg
     7321 cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc atcgcccagg
     7381 caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc gtcaaacgac
     7441 taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga ccagcaataa
     7501 ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg gtgaaaagac
     7561 gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcaggg
     7621 gaatttcaac tattacctta gtgactatcg cagaaaaaac aaacggcacg acacagctgt
     7681 cgtaccgtta ttgcttattg ctattgctgc gctttcgttg ctgcgccaga aatcgcgtta
     7741 ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt taatactgtt
     7801 gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac gtcttccttt
     7861 taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat cgccaaaaat
     7921 gacggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg cgagagatgg
     7981 agttgccgag atgtttgatg tcttcgccga aaccgcgagc ggtgttacag cccgtgagca
     8041 gcgtactggc aagcaaaaca agaacgataa ggcgtttcat catctctgac ctgaataagt
     8101 gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag acgtattcac
     8161 cagagcgggt atccactttg atgacttcgc cgatttgtac gaacagcgga accttaacca
     8221 cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca cctttcaggc
     8281 ccggatcggt atcaacgatt tccagttcaa cgaagttcgg cggagtaacg gagatcggct
     8341 gaccattcca cagagttacg atacactctg cctgatccag cagccattta gcgttatcgc
     8401 caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg aagtgccaga
     8461 actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagcg ccttcagcgg
     8521 aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga cgcagtttaa
     8581 cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca accgcgtaag
     8641 gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag tacgttgcca
     8701 taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca tattgtaacc
     8761 ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt gaccgatcct
     8821 gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc cggacgcagc
     8881 gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc
     8941 aatccggacg atcctctttt gcgtcaggta cttacctcgc aagatgagtt tgtcgtcgcg
     9001 tccggattct ccaccgaccc actggaagaa cagcacagcg tagtgcctgg tttgttgcat
     9061 aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat
     9121 tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa ctggcagact
     9181 gcacttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc cggcggcgat
     9241 ccgctgatgg cgaaagatca cgagctggac tggttgctca cacaactgga agccatcccg
     9301 catatcaaac gtctgcggat tcacagtcgt ctgccgattg tgatcccggc acgcatcaca
     9361 gatgcgctgg ttgaacgctt ttcacattct acgctgcaaa tcctgctggt gaatcacatc
     9421 aaccatgcca atgagataga tgaaacattc cgtcaggcga tggctaagtt gcgccgggta
     9481 ggcgttactt tgctgaacca gagcgttctg ttacgtgatg tgaacgataa cgcacaaacg
     9541 ctggcaaacc tgagtaatgc gttgttcgat gccggcgtga tgccgtatta cctgcatgtg
     9601 ctcgataaag tacagggcgc agcgcatttt atggtgagtg atgacgaagc acggcagatt
     9661 atgcgtgagt tgctgacatt ggtgtcggga tatctggtgc cgaaactggc gcgagaaatt
     9721 ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagtt actgattaaa
     9781 cagggaagtt aaacattaaa attgcctgat gcgctacgct tatcaggcct acatcgtgca
     9841 ttgaaattga atggatttgc aatgaactgt aggccggata aggcgtttac gccgcatccg
     9901 gcaagagcaa caaacgcaag tacagcaacc tgtcattaaa ctgagaaatt taatctttgc
     9961 ccgttgcata acctttaatt attcatatgc attgctgttg ttacttttta tatagctaac
    10021 accaataatt aacctttctt ttctgtttat atagagtgag cacggctatt tatgttattt
    10081 taacaactgt tactaccaag cccatttatc tggcttataa attaaaatta attatttaat
    10141 tgagaaatgg ttagggagaa cctacatggc cataagcatc aagggtgtta ataccggtgt
    10201 tattcgcaag agtaataatt tcattgcact ggcgctgaaa attaaagaac ctcgcaataa
    10261 agaatcgttg ttctttatgt ctgtcatgga actgcgcgac ctgctgattg cgctggaaag
    10321 tcgtctgcat caaaagcata aactggatgc tgctgcacgc cttcagtatg agcaagctcg
    10381 cgataaagtc attaagaaaa tggcagaaaa catcccagaa attctggttg atgagcttaa
    10441 aaatgccgat attaatcgtc gggttaacac gctggaactt accgataatc aaggcgaaaa
    10501 tcttaccttc gtattaacgc tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt
    10561 gcagattgaa atgctagcgc gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga
    10621 actggcatta cgtattacct cactgttaga tttcctgccg ttatatgacg ttgactgcca
    10681 ggaaaatggc aatctggaat atgacaccta ttcccaaccg gaatggaaac ataacttgtt
    10741 cgaccattat ctggcggtgc tttaccgctt taaagatgaa agtggcaaag aacaatttag
    10801 cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa gaagtagagg cgatcactcg
    10861 ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta
    10921 tgttcgcacc gtagcggcga ataacgccca accgctaacc caggatcaat gcttacgcgc
    10981 gctgcatcac ttacgtgttc aatccaccag caaaactgca ccacaggcga aataatctca
    11041 tctttgctaa tgcgaggctg gagccatcca gcctgaaatg actgataacg tgatcgttgg
    11101 tcatgccgga tgcggcgtaa acgccttatc cggcctacaa attctcaagc attcaatcag
    11161 ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca ggcaataata cgtttgtcac
    11221 ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat ctggctatcc
    11281 gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta
    11341 ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc
    11401 tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt accaatcagc
    11461 tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc agcactcaat
    11521 tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat
    11581 tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc
    11641 tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt
    11701 ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc
    11761 ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg gtaaccatgc
    11821 actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta
    11881 ctttggttgg atccaagata cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt
    11941 tgtaaccgta gttgccgtcg ccgcctttaa cagtgttagc aacaacagac ggttcttcgc
    12001 cgcagttcaa tacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac
    12061 ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca
    12121 gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg
    12181 catcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca cctactttga
    12241 taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca cggtcgtagt
    12301 cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt
    12361 cttcacccac gccatcaatg atggtggtgg tgtctttgtt gatcacaaca cgtttagcct
    12421 gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca gagattacgg
    12481 taccgccagt cagggttgcg atatcctgca gcatagcttt acggcgatcg ccgaagcccg
    12541 gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca
    12601 gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg tttacctgct ttagcaacgg
    12661 cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga
    12721 acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt
    12781 agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt
    12841 cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac
    12901 ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct ttagagtcag
    12961 agcatggtac ggacagcgct ttcagttctt caactgcagc ggtaacagct ttgtcgatac
    13021 cacgtttcag gtccatcggg ttcatgcccg cagcaactgc tttcagaccc tcagtgatga
    13081 tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttag
    13141 aggcaacttc tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc agttcgattt
    13201 cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa
    13261 ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt acgtttacgc
    13321 cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct
    13381 ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt acgcttcaac aattgccaga
    13441 atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg
    13501 tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca
    13561 ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct
    13621 gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg
    13681 atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta
    13741 tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg
    13801 atgaggattc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg
    13861 gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt cataagcgaa
    13921 tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggata
    13981 tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg gcctgctatc
    14041 gacgtcatta ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac aggtagcagg
    14101 caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt
    14161 gtttgcgatt ctgggtcgcc actatccctg cgcaggcggt gtcgcgcact tcgtcggtat
    14221 ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg tcattcccgt
    14281 gggtttgcca gccgcgctac aaattgccgc cgggttcggc caggcaatgt ttggctggca
    14341 tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt atatcggtac
    14401 tcgcagtgcc agttccagtg ctaatctaca aaccgttatt gccggactta tcgtcgcgct
    14461 gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct tcccggcacc
    14521 tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt
    14581 cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc
    14641 tcgcgcattg atgattggtc tgctgctggc aggattagtc tactggggct gtacggtagt
    14701 cgtcttacac ttcgacgcct atggtgaaaa aatggcggcg gcagcatcgc ttccaaaaat
    14761 tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtgattg gctatctggc
    14821 ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc
    14881 gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc
    14941 cctcaatgcg gtgctcggct gctgtgtggt gagcactttg gtgattcatg ctttagagat
    15001 caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg
    15061 catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg
    15121 gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg cgctgatcgt
    15181 gctggcgggg ttatggctgt tgctgccaaa acgaaaaacg ccggaaaatg gcataatcac
    15241 ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg cggtcgcgct
    15301 catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa
    15361 aaccgccgcc tggcatacga gaaaagcgca aatgcggcat caacttcact gtcagatgct
    15421 tttgcaccgg cggtaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata
    15481 aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc gctgggtttt
    15541 cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca
    15601 gtgacatccc gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata
    15661 catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa
    15721 aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gtaaaacccg gctacctgaa
    15781 tgggttgcga atcgcgttca gcttatattg tggtcattag caaaatttca agatgtttgc
    15841 gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta ataattaagg
    15901 ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag cagcatatga
    15961 tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat
    16021 atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa
    16081 caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta
    16141 tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga
    16201 tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa
    16261 agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt
    16321 cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg
    16381 tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg
    16441 tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc
    16501 cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaagct
    16561 ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga
    16621 catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga
    16681 attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aatatccaac gtaccgctga
    16741 actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa
    16801 agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc
    16861 ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg ttcacggcgc
    16921 gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc
    16981 tggcccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg gctcttccat
    17041 catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt
    17101 catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt
    17161 tatggagccg gtcattggcc aggccatgtt cgaatccgtt cacattctga ccaacgcttg
    17221 ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg
    17281 ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa
    17341 cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct
    17401 ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat
    17461 gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt
    17521 agtacaaata aagaaggcac gtcagatgac gtgccttttt tcttgtgaac agtaacttaa
    17581 aaacaacaac ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga
    17641 actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg
    17701 ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat
    17761 cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt
    17821 tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa
    17881 atacatcacg atcctcgcac ctatcgtgac ctatttcctg acaatctttg ctggtactgg
    17941 caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa
    18001 accttgccgt ccgctctcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc
    18061 aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct
    18121 ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtcctttct
    18181 ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga
    18241 agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac
    18301 gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag
    18361 cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa tcctgatcat
    18421 catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacatcct
    18481 caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg
    18541 gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt
    18601 gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta
    18661 ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttcacc
    18721 gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc cgacctaccc
    18781 gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg gtaaattcgt
    18841 cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg
    18901 cttcgtgctg ggtagcttca tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt
    18961 ctttcccgcc gactaacatc cttcccccgt acgttgtata gtgacctctc tcttgcggtt
    19021 ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg
    19081 tggtgctatg tacggcacca gatgaagcga cagcccagga tttagctgcc aaagtgctgg
    19141 cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc tattactggg
    19201 aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc gtatctcacc
    19261 agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg gaacttctgg
    19321 ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca tctttacgct
    19381 gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc cgggacgttc
    19441 acaatttgtc cccgcggatc aagcctttac ttttgatttt cagcaaaacc aacatgacct
    19501 taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga tccgcattac
    19561 gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc atgaagatga
    19621 gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa ccatcaacca
    19681 ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg ccggtttctg
    19741 ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc
    19801 acagcctgtg tctgtttcgc agcaagagca gcacaccgcg caattgccct tttccgcgct
    19861 ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc
    19921 actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca gagcattgtt
    19981 gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg gtctggtagt
    20041 tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc tcattggcct
    20101 cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc tacaactccc
    20161 ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc caacagggcg gctcacctgg
    20221 cggtgtgttt gttatggggg cgattgccgg acttatctgt tcaccatgca ccaccgcacc
    20281 gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg gcggcggcac
    20341 gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg tctttggtaa
    20401 ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt
    20461 gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatgtat ggggattacg
    20521 cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca gcctacaggc
    20581 taaacgcggt tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat tggttagcgt
    20641 gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc agacgcatct
    20701 caactttaca caaatcaaaa cggtagatga gttaaatcat gcgctcgttg aagccaaagg
    20761 caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa
    20821 atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct tacttcaggc
    20881 caacatcacg gccaacgacg cacaagatgt ggcgctgtta aagcatctta atgtccttgg
    20941 cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag cacgcgtcac
    21001 gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac
    21061 acttgcagcg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc
    21121 cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg ttgctgaacg
    21181 tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg caatcctcta
    21241 cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc tgatgctgga
    21301 cgaaacgcaa accgccgaac aaaagcagct ggcacgttat caggcgttat cggagtgcgt
    21361 taaaaacaac cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc
    21421 tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg atttcaccca
    21481 cgaactgtta accacactgg aagttgacga tccggcgatg gtagcaaagc agatggaact
    21541 ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac
    21601 tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt
    21661 gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt
    21721 ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc gttgcccgga
    21781 tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg attccgagtc
    21841 cgggcaccaa attcatatca acggacctct acggaggttc gtttttcgtt tcagaacgcc
    21901 acgatttaag cgttctgcct acaaatcaat tctaccgaac tcaaccagat tccccccaca
    21961 tcaaccccat tgtgtgggta taattgcggg cataccccag ttcgacagaa tttgtaccct
    22021 ctttaacgca ttcagaaggc tgaactatgg cactgactga cgcaaaaatc cgggctgcaa
    22081 agcccactga caaggcttat aaactcactg acgggactgg catgttcctg ctggtacatc
    22141 ctaatggctc ccgttactgg cgtctccgtt atcgtattct gggtaaggag aagactctgg
    22201 cacttggtgt gtatccagaa gtttctctct ccgaagctcg tacaaaacgg gatgaggccc
    22261 gaaaactgat ttcggagggg gttgaccctt gcgaacagaa aagagctaaa aaagtagtcc
    22321 ctgatttaca gctctctttt gaacatattg cgcgacgctg gcatgccagt aataaacaat
    22381 gggcacaatc acacagcgat aaagtactca aaagcctcga gactcacgtt ttccccttta
    22441 tcggcaaccg ggatatcaca acactcaata ccccggatct gcttatccct gttcgtgctg
    22501 cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata tctgccgtaa
    22561 tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat atggctggcg
    22621 cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc ctgcctgaac
    22681 ttttgtcgcg tattggcagt tacaaagggc aaactgtcac ccagcttgcc gttatgctga
    22741 atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct gaaattgata
    22801 ttgacaatgc catgtggact attccagccg aacgcgaacc cctgccaggc gtaaaattct
    22861 cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa caggctgtag
    22921 ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt acgggagcac
    22981 atgacccgcg taaaccaatc agtgaaaatg taatgaacgc atc
//
LOCUS       DAJVTY010000051        22847 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVTY010000051 DAJVTY010000000
VERSION     DAJVTY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22847
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.051"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..658)
                     /gene="fsr"
                     /locus_tag="OQQ66_001812"
     CDS             complement(<1..658)
                     /gene="fsr"
                     /locus_tag="OQQ66_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1904774.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     P"
     gene            876..2528
                     /gene="ushA"
                     /locus_tag="OQQ66_001813"
     CDS             876..2528
                     /gene="ushA"
                     /locus_tag="OQQ66_001813"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1904775.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2565..3044)
                     /gene="ybaK"
                     /locus_tag="OQQ66_001814"
     CDS             complement(2565..3044)
                     /gene="ybaK"
                     /locus_tag="OQQ66_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1904776.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3248..4042)
                     /locus_tag="OQQ66_001815"
     CDS             complement(3248..4042)
                     /locus_tag="OQQ66_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1904777.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4180..4521
                     /locus_tag="OQQ66_001816"
     CDS             4180..4521
                     /locus_tag="OQQ66_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1904778.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4736..7240)
                     /gene="copA"
                     /locus_tag="OQQ66_001817"
     CDS             complement(4736..7240)
                     /gene="copA"
                     /locus_tag="OQQ66_001817"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1904779.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7502..8434
                     /gene="glsA"
                     /locus_tag="OQQ66_001818"
     CDS             7502..8434
                     /gene="glsA"
                     /locus_tag="OQQ66_001818"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1904780.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8437..9729
                     /locus_tag="OQQ66_001819"
     CDS             8437..9729
                     /locus_tag="OQQ66_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1904781.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9854..10261
                     /gene="cueR"
                     /locus_tag="OQQ66_001820"
     CDS             9854..10261
                     /gene="cueR"
                     /locus_tag="OQQ66_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1904782.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10262..10720)
                     /locus_tag="OQQ66_001821"
     CDS             complement(10262..10720)
                     /locus_tag="OQQ66_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1904783.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10717..11634)
                     /locus_tag="OQQ66_001822"
     CDS             complement(10717..11634)
                     /locus_tag="OQQ66_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1904784.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11780..12457
                     /gene="fetA"
                     /locus_tag="OQQ66_001823"
     CDS             11780..12457
                     /gene="fetA"
                     /locus_tag="OQQ66_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1904785.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12444..13223
                     /gene="fetB"
                     /locus_tag="OQQ66_001824"
     CDS             12444..13223
                     /gene="fetB"
                     /locus_tag="OQQ66_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1904786.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13286..14140)
                     /gene="cnoX"
                     /locus_tag="OQQ66_001825"
     CDS             complement(13286..14140)
                     /gene="cnoX"
                     /locus_tag="OQQ66_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1904787.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14201..15010)
                     /gene="ybbO"
                     /locus_tag="OQQ66_001826"
     CDS             complement(14201..15010)
                     /gene="ybbO"
                     /locus_tag="OQQ66_001826"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1904788.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15000..15623)
                     /gene="tesA"
                     /locus_tag="OQQ66_001827"
     CDS             complement(15000..15623)
                     /gene="tesA"
                     /locus_tag="OQQ66_001827"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS1904789.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15594..16280
                     /locus_tag="OQQ66_001828"
     CDS             15594..16280
                     /locus_tag="OQQ66_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1904790.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16277..18688
                     /locus_tag="OQQ66_001829"
                     /pseudo
     CDS             16277..18688
                     /locus_tag="OQQ66_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19117..22847
                     /locus_tag="OQQ66_001830"
                     /pseudo
     CDS             19117..22847
                     /locus_tag="OQQ66_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5261 a         5988 c         6466 g         5132 t
ORIGIN      
        1 gtggattgat tatcgtcgct ttgggttttc ctttattcat tcggtgctgt gccgagtacc
       61 aacggctaat ttgcgccaac accacgatcg ccagcagtgc cgcaagcaca aaccaggcaa
      121 cgttgccttt gccataaggc gcgataatca ccgccgtcag caagggtccc agggaactgc
      181 caaagttgcc gccgacctga aagatagatt gcgccaggcc atgccgcccg ccggaagcca
      241 tacgggctac gcgagaagat tccggatgaa agaccgatga actggtaccg accagcgccg
      301 ccgccagcag aactgcgcca acactgcccg ccagcgcaag cagcaccaga ccacttaagg
      361 taaagcacat gccaattggc aacgaccacg gcatcggata tttatcggtc cagtagccga
      421 ccactggttg cagtagcgaa gaggcgagct ggaaggtgag ggttatcatg ccaatctgca
      481 taaatgtcag agaaaattct gactgaagca gaggataaat cgccagaatc agcgattgga
      541 tcatgtcgtt cagcagatgt gagaggctga tagcacctaa aataccaaac gatgttcggg
      601 ccttggtcgt tgacgcagcc gcgcccgcca caggctgggt ttgttcactc attgccatag
      661 gaaagtcact ttttcagggt tgcgatgtaa agaatgatct tatttgtgat tattacccga
      721 ctaacatacc tgtaagcgtc gcctgaagga agtctcaacg ccgaatacag aatttctaat
      781 ctggatgcag atttatcttc accggacgca gacttgtcta ttatgttgcg tcatactatt
      841 tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa attattgcag cggggcgtgg
      901 cgttagcgct gttaaccaca tttacactgg cgagtgaaac tgctctggcg tatgagcagg
      961 ataaaaccta caaaattaca gttctgcata ccaatgatca tcatgggcat ttttggcgca
     1021 atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct ggtggatggt atccgcaaag
     1081 aggttgcggc tgaaggcggt agcgtgctgc tactttccgg tggcgacatt aacactggcg
     1141 tgcccgagtc tgacttacag gatgccgaac ctgattttcg cggtatgaat ctggtgggct
     1201 atgacgcgat ggcgatcggt aatcatgaat ttgataatcc gctcaccgta ttacgccagc
     1261 aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat ctaccagaaa agtaccggcg
     1321 agcgcctgtt taaaccatgg gcgctgttta agcgtcagga tctgaaaatt gccgtcattg
     1381 ggctgacaac cgatgacaca gcaaaaattg gtaacccgga atacttcact gatgtcgaat
     1441 ttcgtaagcc cgccgatgaa gcgaagctgg tgattcagga gctgcaacag acagaaaagc
     1501 cagacattat tatcgcggcg acccatatgg ggcattacga taatggtgag cacggctcta
     1561 acgcaccggg cgatgtggag atggcacgcg cgctgcctgc cggatcgctg gcgatgatcg
     1621 tcggtggtca ctcgcaagct ccggtctgca tggcggcaga aaacaaaaaa caggtcgatt
     1681 acgtgccggg tacgccatgc aaaccagatc aacaaaacgg catctggatt gtgcaggcgc
     1741 atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc gaaatgaaaa
     1801 tggttaacta ccagctgatt ccggtgaacc tgaagaagaa agtgacctgg gaagacggga
     1861 aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa ccagcaaatg atctcgctgt
     1921 tatcgccgtt ccagaacaaa ggcaaagcgc agttggaagt gaaaataggc gaaaccaatg
     1981 gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gaccaatatg gggcggttga
     2041 ttctggcagc ccaaatggat cgcactggtg ccgactttgc ggtgatgagt ggaggaggaa
     2101 ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtactgaaa gtgcagccat
     2161 tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga ggtgattgat tacctgaccg
     2221 ccgtcgcaca aatgaagcca gattcaggtg cctacccgca atttgccaac gttagctttg
     2281 tgacgaaaga cggcaaactc aacgacctga aaatcaaagg cgaaccggtc gatccggcga
     2341 aaacttaccg tatggcgaca ttaaacttca atgccaccgg cggtgatggc tatccgcgcc
     2401 ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg ctgaaagcgt
     2461 atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt gaggtgagct
     2521 ggcagtaatc cgaaagtgcc ggatatttgc atccggcaca atgcttaatc gcggcgggcg
     2581 atatcagcaa atttggcatc gaggatcttc gccagatcgc ctgccgccag ttcgatatcc
     2641 agtccgcgct tgccgccaga aacataaata gtggcaaatt cttgtgcggg ggcgtcgata
     2701 atcgtcggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag gtatcccgtc
     2761 gaacgctgcg cggccatcgg atcggccatc tcaactttct tggcacccag cgcttttgct
     2821 acttttttaa gatccagttg acctgcgacc ggcgtaacgg ccactgccag atgtttcata
     2881 tcaccgttca ctgccaccag tagcgttttg tagacttgat ccggattcaa acctaatttt
     2941 ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt atggatttga
     3001 aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat tttcctgcct
     3061 taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag agtcatcttg
     3121 cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc gtcgcttaaa
     3181 gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag cgatattggc
     3241 cattttttta gcgcaacatt tgcggcaaat tcccttctcc atacaggtgt agtgcaccga
     3301 ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag gcgagatttc
     3361 gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc gttatatcat
     3421 tattttgcgg aggtgcattc agccaccagc tcatcatttg ttgcagcaac cgtgcgttgg
     3481 tatgccagtg ggtcagcgta tcgtccagca gcgccagtcc tttgtcaggg agctggagca
     3541 acatggtaat ctggttttca gccccttcca gttcaatcac gggtttatgt tgttgcttcg
     3601 ccgcctgcaa taactggtaa tcgataccgt attctgcccg cagccccagt ttttgtgcct
     3661 gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg agtagaaaag agcgacggag
     3721 aaatgcccat ctcctggcta atgtgctgca gattttggag ttgctcctcg ttaatgcgct
     3781 cttccagcgc ctcgcaagta ggcaaattag caaaaggcgt atcgctggtg gaaacatccg
     3841 cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg ggcaggggag
     3901 tcatatcgtg gctacccata tgaatactgc caatcagatg aaaatggcga ttgccgggaa
     3961 gggtgatatc gatggcaggc caggtgtaat gattaccgcg cagcgcggcc caaagcgttt
     4021 ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct agcgcgcggt
     4081 gagggatggc gcaaatggag ttggggactt gtttaatgtg tttgtatgat tcagtatgtt
     4141 cttgcatcgc tattcacagg gaagcaacag ttaaaaacca tgaaacaggc aacaagaaaa
     4201 ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga tttgaaaatc
     4261 aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtacact gatcaataat
     4321 aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga taccacagtc
     4381 gatttttggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa caacatgcgc
     4441 acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg ccgtgaagag
     4501 cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat ctcgtaggcc ggataaggcg
     4561 ttcacgccgc atccggcatc cgagcaccat tgcctgatgc gacgcttacc cgtcttatca
     4621 ggcctacaaa cctgtgcctg aaccgtaggc cggataaggc gtttacgccg catccggcag
     4681 tcatgcgtcg atgccaaatg cgccgcccta aagcagcgca tccgcaatga cgtacttatt
     4741 ccttcggttt aaaccgcagc aaccggttgg cgttactcac tacggtaatc gacgagagcg
     4801 ccattgccgc tccggcaact accgggttaa gcagtgttcc agtaaacggc cacaaaatac
     4861 cagcggcgac cggaatacca atgctgttgt agataaacgc accgagcagg ttctgcttca
     4921 tattgcgcag cgttgcgcgg gaaatcgcga gcgcatctgc aacacccatc aggctatggc
     4981 gcatcagggt aatcgccgcg gtttcaatgg caacatcact gccgccaccc atcgcaatac
     5041 cgacatccgc ctgagccagc gctggcgcgt cgttaatgcc gtcgcccacc attgccacct
     5101 gacgtccttc actttgcaga cgtttgatcg cttcggcttt accgtctggc agcaccccgg
     5161 cgattacctc atcaatccct gcttctttgg cgatcgcatt ggcggtggtt gggttatccc
     5221 cggtcaacat caccagacga tatcccgctt tatgcaggcg ttgcagcgcc gccacgctat
     5281 cactacgcaa cggatcgcgt acagccagca gggctactac tttcccgtca atcgccagca
     5341 gcacaggcgt tgctccttgc gatgcctgag ctgaaatatc ggcttcaatc gctttagtat
     5401 cgacttgctg gtcatttaac agcgccggat tacccagcaa taacgcatga ccttcagctt
     5461 caccgcttac gcccagcccg cgcaatgtgc ggaaaccgtt gacctgcggc agctgcatat
     5521 cgcctgcttt atcgaggatc gctcgtgcca gcggatggct ggaaccttgc tccagcgccg
     5581 ccgccagacg caatgcttgc gcttcatcaa catcagcaaa tgtttttact gcgacaacct
     5641 gcggcttccc ttcggtcagc gtcccggttt tatcgaacac gacggtatcg agtgtactgg
     5701 cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa ctcagccgcc cgcccgacgc
     5761 cggaaataat cgacattggc gtcgccagcc ccagcgcaca cggacaggca ataatcagca
     5821 ccgtggtggc tatcaccagg gtatagacaa tctgcggtgc cggaccaaag aaataccaga
     5881 ttgctgcact gatcagcgca atcaccacta ctaccggcac aaatacggct gagattttat
     5941 ccgccagctg accgatttct ggcttgctgc tctgggcctg gcgcaccatg cgaataatcc
     6001 ttgacagcgt ggtatggctg ccaaccgcac tggcgcgaaa tagcacgcta ccgtcctgta
     6061 ccactgtccc ggcatggacg ctttcgcctt cgcctttttg ctgcgggatt ggttcgcccg
     6121 tcagcatcgc ttcatccagc catgcttcgc cctgggtaat ctcgccatct accggcacgc
     6181 gatcgccggt cgtcaggcgc agtaacatcc ctggctgaac ttccgccagc ggcacgcttt
     6241 tttcaccctc tgcggtgacc actcgcgccg tcggtggggt taaatcgagt aatttctcca
     6301 gcgccttaga agaacgctga cgcgcgcgcg cttccagcat atggccgaga ttaatcagac
     6361 cgataatcat cgcactggct tcatagtaaa gatgccgcgc ttccatcggg aaccactgcg
     6421 gccacaggtt gacgctcatc gaatagagcc acgccacacc cgtacccagc gccaccagcg
     6481 tatccatcgt cgccgcaccg ttcagcaggc ttttccacgc gctgcggtaa aaatgcccgc
     6541 cggcgaaaac cattaccgcc agggttatca ggccgataac taaccacagg ctgcggttgt
     6601 cagcggtgac catcatgtta tcgccgatca tcccccagac cattaccgga atacccaccg
     6661 ccagtgcgac aatcgcctgc cagcggaagc gcttcatcgt agcgacggcg gtttcctgct
     6721 ggcgctcgcg gcgtttcgcg tcatcttcaa tcgcttccgc gccgtagccc gctttttcca
     6781 ccgcctgcac taaatcttgt ggggaggcac tgcccatcac cagcgcagta cgctccgcca
     6841 ggtttacccg tgcctgagtg acgcccggta cgctttgcag cgcattttgt acgcgggtga
     6901 cacagctggc gcagctcatg ccgctcagca gcaactgctg gctgtcatcg tcatcggcgg
     6961 tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc cgacgggatt gatgactccg
     7021 ccagcggttt agcctttggg tggcttacag atgcgtcata acccgcttgt ttgatggttt
     7081 cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc ttcagtgata gacacatccg
     7141 cttgttccac atccgggcgc tgttcgagac tttctttcac gcgtttaacg cagtgaccgc
     7201 aggacaggcc gtccagggtc aggtcgatag tttgtgacat aaaacactcc tttaagacag
     7261 ttttgactgg ctgtgataaa ggttaaacct tccagcaagg ggaaggtcaa gaaattaata
     7321 aaccaggcgg gtaaaagtcc gtaaagatta aaaaatcggc tcgatttgca tcaggattag
     7381 acatttattt ctttgttttc ctgtagttaa gttgctggtg ctaagttaaa tcaggatgcc
     7441 tgaaaatcgg caccggggtg aggaattacc tcccgcatct ataaaaagga gttaacaaaa
     7501 gatgttagat gcaaacaaat tacagcaggc agtggatcag gcttacaccc aatttcactc
     7561 acttaacggc ggacaaaatg ccgattacat tccctttctg gcgaatgtac caggtcaact
     7621 ggcggtagtg gctatcgtga cctgcgatgg caacgtctat agtgcgggtg acagtgatta
     7681 ccgctttgca ctggaatcca tctcgaaagt ctgtacgtta gcccttgcgt tagaagatgt
     7741 cggcccgcag gcggtacagg acaaaattgg cgctgacccg accggattgc cctttaactc
     7801 agttatcgcc ttagagttgc atggcggcaa accgctttcg ccactggtaa atgctggcgc
     7861 tattgccacc accagcctga ttaacgctga aaatattgaa caacgctggc agcgaatttt
     7921 acatatccaa cagcaactgg ctggtgagca ggtagcgctc tctgacgaag tcaaccagtc
     7981 ggaacaaaca accaacttcc ataaccgggc catagcctgg ctgctgtact ccgccggata
     8041 tctctattgt gatgcaatgg aagcctgtga cgtgtacacc cgtcagtgct ccacgctcat
     8101 caatactgtt gaactggcaa cgcttggcgc gacgctggcg gcgggtggtc tgaatccgct
     8161 gacgcataaa cgcgttcttc aggccgacaa cgtgccgtac attctggccg aaatgatgat
     8221 ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc gttggtttac cgggcaaaag
     8281 cggtgtaggt ggcggtattc tggcggtcgt ccctggcgtg atgggaattg ccgcgttctc
     8341 accaccgctg gacgaagaag gtaacagtgt tcgcggtcaa aaaatggtgg catcggtcgc
     8401 taagcaactc ggctataacg tgtttaaggg ctgatcatga tgaacacgga aggtaataac
     8461 ggtaacaaac ccctcggtct atggaatgtc gtttccatcg gtattggggc aatggtgggg
     8521 gcggggatct tcgcgctgct ggggcaggct gcgttgctaa tggaagcctc gacctgggtc
     8581 gcctttgctt ttggcggtat tgtggcgatg ttttccggtt atgcctatgc gcgtctgggg
     8641 gcgagctatc ccagcaatgg cggcattatc gacttctttc gtcgcggatt aggcaacggc
     8701 gtcttttcgc tggcgctctc gttactgtac ctgttgacgc tggcggtgag catcgccatg
     8761 gtcgcccatg cttttggcgc ttatgccgtg cagtttttgc atgaaggcag ccaggaggag
     8821 caccttattt tgctctacgc gttggggagc attgcggtga tgacgctttt caactcctta
     8881 agcaaccatg cggtagggcg gctggaagtg atcctcgtcg gcattaaaat gatgatcctg
     8941 ttattgctga ttattgccgg tgtctggtcg ctgcaaccag cgcatatttc cgtctctgcg
     9001 ccccccagct ccggtgcgtt cttctcctgt attgggataa ctttccttgc ctatgcgggc
     9061 tttggcatga tggcgaacgc ggcggataaa gtgaaagatc cgcaggtcat tatgccacgg
     9121 gcgtttctgg tggcgattgg cgttaccacg ttgctttata tctcgctggc actggttttg
     9181 cttagcgatg tatcggcatt agagttagaa aaatatgccg ataccgccgt agcgcaggct
     9241 gcttctccgc tgctcggaca tgtgggttat gtgatcgtcg tcatcggcgc tttactggcg
     9301 acggcttcag ccattaacgc gaacctgttc gccgtgttta acatcatgga caacatgggc
     9361 agcgaacgcg aactgccgaa gctaatgaat aaatccctgt ggcggcagag tacctggggc
     9421 aacatcattg tcgtggtgtt gattatgctg atgacggcgg cactgaattt aggctcactc
     9481 gccagcgttg ccagcgccac ctttttgatt tgctacctgg cggtgtttgt ggtggcgatc
     9541 cgcctgcgtc atgatattca cgcctcgttg ccgattctta tcgttggtac gttggtgatg
     9601 ttgttggtga tcgttggctt tatctacagt ctgtggtccc agggtagccg tgcgttgata
     9661 tggattattg gctcactctt actcagcctt attgtggcaa tggtcatgaa gcgcaataaa
     9721 accgtataac atctctctgt gcgcagtact tcctgtatta ttgtggtggc ggtcgatatt
     9781 cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc ctttaacaaa gcacaggagg
     9841 cgttgcgcga acgatgaaca tcagcgatgt agcaaaaatt accggcctga ctagcaaagc
     9901 cattcgcttc tatgaagaga aggggctggt gacgccgccg atgcgcagcg aaaacggtta
     9961 tcgcacctac acgcagcagc atctcaacga actgacctta ctgcgccagg cacggcaggt
    10021 gggctttaac ctggaagaga gcggcgagct ggtgaatctg tttaacgacc cgcagcggca
    10081 cagcgccgac gtcaaacggc gcacgctgga gaaggtggcg gagatcgaac gacacattga
    10141 ggagctgcaa tccatgcgcg accagctgct ggcactggcg aatgcctgcc ctggcgatga
    10201 cagcgccgac tgcccgatta tcgaaaatct ctccggctgc tgtcatcatc gggcagggtg
    10261 attaagacga gacggctcgg atatgtaggg ttatcccttc tatcgcaatg acttcaacat
    10321 gcgtacctgc accgagatcc tcgctggcgc tgacaggcca tgaactgtca ccgacgcgca
    10381 tatgaccgcg cccgttgacc agcggagatt ccagcacaaa acgtcggcca atcagctgct
    10441 gcccgcgctg gtttaaatga ctatcgctgt gcttttgttc gcgcacccgc cgcgacaacc
    10501 atttccacca cagccaggcg gcgagcagcg tcaggatggc aaacattacc ccttgccact
    10561 cccaacccag cggcaccagc cagaccacca ggccggtaat caccgctgcc acgccgctcc
    10621 acaacaaata accatttccg cccagcatct cggctgccag cagcaaaccg ccgagactga
    10681 gccagaaaat atgtggatga acgaccatta actccatcat ggctgagtcc gtttgttggc
    10741 gctgtctttc accagttcgg caatcccggc aatcgacccc atcaggctgc tggcctctaa
    10801 tggcatcatc actactttgc tgttactgga ggaaccgatc tgctgtaacg cttcggtgta
    10861 tttctgcgct acgaagtagt tcaccgcctg aatatcaccg gaggcgatag cttcagacac
    10921 cattttggtg gcgcgggctt ctgcttcggc ggaacgttca cgcgcttcag cctgtaaaaa
    10981 cgccgactga cgttcgcctt ccgctttcag gatttgcgac tgtttttcac cttcggcttt
    11041 gaggatttcc gcctgacgga tcccttccgc ttcaagaatg taagcgcgtt tggtacgttc
    11101 cgctttcatc tgcgcgttca ttgaagagat aagctctgcc ggtgggcgca cgtcgcgaat
    11161 ttcaatacgg gtgactttaa tcccccacgg gttggtggct tcatcgacaa tacgcagcag
    11221 gcgtgagttg atgctgtcgc gctgagagag catttcgtca agttccattg aacccaacac
    11281 ggtacggatg ttagtcatgg tcaggttgat gatcgccagc tccagattgc tgacttcata
    11341 ggcagcgcgt ggcgcgtcaa tcacctgaat aaaacagact gcgtcgatgg taacgttggc
    11401 gttatctttc gagataactt cctgggaagg gatatcgagc acttgctcca tcatattgat
    11461 cttgcgacca atgcgatcca taaacggcac caccagactg agccccggct gtaacgtttt
    11521 ggtatagcga ccaaaacgtt ctaccgtcca ttgatagccc tgcggcacga ttttgacgcc
    11581 cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg ataaagataa gcatcggaaa
    11641 aacctcctgt tgtaccgtcc ataatcagca aaattgctgc ttgattaaac aaattatacc
    11701 tgattactga aagagagttc ccccttattc ctgcgaagga taaactgttt ttagtaaaaa
    11761 tcagaaaaag ggaacagcga tgcaggaaaa tagtcctttg cttcagctac aaaacgtagg
    11821 atatctggcg ggtgatgcga agattcttaa taacatcaat ttttcgctgc gtgctggcga
    11881 atttaagtta attaccggtc cttctggttg tggcaaaagt acgctgctaa aaatagttgc
    11941 ttcattgatc agcccaacca gcggaacgtt actgtttgaa ggtgaggatg tcagcacact
    12001 aaagccagaa atctaccgcc aacaagtctc ttactgcgcc cagacaccga cgctgtttgg
    12061 cgatacggta tacgataatc tgatctttcc ctggcagatc cgtaaccggc agcctgaccc
    12121 agccattttt ctcgattttc tcgaacgctt cgccttgccg gacagcattt tgacgaaaaa
    12181 tatcgccgag ctatctggtg gtgaaaaaca acgcatctca ctgattcgta acctgcaatt
    12241 tatgccgaag gttttattgc tggatgaaat caccagtgcg ctggatgaaa gtaataaaca
    12301 taacgttaat gagatgatcc atcgttatgt gcgcgagcaa aatattgccg tgctgtgggt
    12361 gacacacgat aaagacgaaa ttaatcatgc ggataaagtg attacactgc aaccgcatgc
    12421 cggagaaatg caggaagcac gctatgaact cgcataatat tactaacgaa tcattggcac
    12481 tggcattaat gctggtggtg gtggcaatct taattagcca taaagaaaaa ctggcgctgg
    12541 agaaagatat tttctggagc gtcgggcgcg cgataattca gctgattatt gtcggctatg
    12601 tgctgaagta tattttcagc gtggatgatg ccagcctgac attattgatg gtgttattta
    12661 tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa atatattgct aaagctttta
    12721 tctcatcgtt tattgccatt acggttgggg cgggaattac cctggcggtg ctgattctct
    12781 cagggtcgat tgaatttatc ccgatgcagg tgatccctat cgccgggatg attgccggta
    12841 acgccatggt agcggtgggg ttgtgttaca acaatttagg gcaacgggtc attagcgaac
    12901 agcaacagat tcaggagaaa ctgagtcttg gcgcgacgcc gaagcaggct tcagcgatat
    12961 tgattcgcga cagtattcgc gcggctttaa ttccgacggt cgattcagca aaaacggttg
    13021 gcttagtgag tttaccagga atgatgtccg ggctgatatt tgccgggatt gatccggtga
    13081 aggcgattaa atatcagatt atggtgacct ttatgctgct ctcaaccgcc agcttgtcga
    13141 ccattattgc ctgctattta acctatcgta agttttataa ttcgcgccac cagttggtgg
    13201 tgacgcaatt gaagaagaaa tgatgcgatg ccggatgcgc agcatcgcat ccggcgttgt
    13261 ggttcatgtg ccgaacaacg gaatatcaat acaacaatgc atacagctgg cggcgatact
    13321 tcgacgccag tgcatcaccc gtacccagcg cagcgaggat ctcctggaac gttttacgcg
    13381 tctgaccgtc tgcggcggtg agatctttac gcagatgccc gaacagcaac tccagcgcct
    13441 cttcattgcg cccaacctga tgcagttgca gcgccagttg cgtcgccagt gcggcatctt
    13501 ctggattctc cgccacctgc tgttgcaact gttgaatttc cggcgtatca gccgcctgct
    13561 tcagcagttc gatttgcgcc accagcccct ggtagcgggt gtcctgatcc tgcaacggaa
    13621 tggttttcag caccgcttcc gcatcttcag aacggttcag cgcaatcagc gtttctgcca
    13681 gcagcaggcc gatctccccg ttctgattcg acaactgcca ggcgtctttc agcaatggca
    13741 gggcatcggt gtaattgcct tcctgcatca gttgcatcgc ctgctgcgct ttcagctctt
    13801 cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc ctcttccggt tgcggcccct
    13861 ggaagccatc taccggttgc ccgttctgga acagatacac ggtcggaatc gcgcgcagac
    13921 caaactgggc ggcaatcatc tgctccgcgt cgcagtccag cttcgccaga ataaattgcc
    13981 cgttgtactg cgccgcgagg ctttccagga tcggggttaa ctgcaaacag tgctggctac
    14041 gttcagacca aaaatagaac agcaccggag tggtcatcga ctgttcaaga acctgttgca
    14101 ggttagattc gttaatgttg acaatatttt ctacggacat ggagtcgctc tctgttgtcg
    14161 atattttctt tgacatgggg gcttaagcgc gcgcttcaac tcacccctgc aatattttgt
    14221 ccatcacgcg cccaggcagc aggcgcttga gcaccattac tgcccaggtc accagcgtta
    14281 ccggatagcg catcttcggc ttctcgctaa taaaagcatg gcgcactttg tcgaccaccg
    14341 cttccggtcc caacgtaaag cgggcggcga tgccgggatt ttcgactggt ttatcacttt
    14401 gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg accgggttcg atcaggctga
    14461 ctttaattcc gctgtggcgt agctccatgc gcagcgcgtc tgaccacgcc tccagcgcat
    14521 atttactggc cgcgtaagcg ccacgacccg gcgtggagat taatcccatc accgatgatg
    14581 tcatcacaat acgcccttca ccgtgcggta acatcgcggg taacaggcgc atggtgagct
    14641 ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat ctgcgcacgg ctgatggtgg
    14701 aaagggggcc atacatgccg aatccggcat tgttaaagat cccatacaga caattatcgg
    14761 tcagggcgat cacctcgtct gctgcgcgat caacactttc tggtgaatcc agatcgatca
    14821 acacgccggt aaatcccatg ttgttcatac gctcaacatc atccggtttt cggcaacctg
    14881 ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct ttccaggcca attccactgg
    14941 aacatccggt aattaagacc gatttttgca taactttacc tgtcaggatc tccgttgctt
    15001 tatgagtcat gatttactaa aggctgcaac tgcttcgcca tccagtcggc aataaacggc
    15061 tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc attgtggctt gaggtagacc
    15121 tcttccataa aaaagggcag cagcggaaca tcaaactctt tggcgagttt ggggtaaatg
    15181 gcgctaaagg cttcattata acggcgacca tagtttgcag gcagacgtat ttgcattaac
    15241 aatggttcag cgttggcggc tttgacatcc tgcaaaatct ggcgcagcgt ttgctcggtt
    15301 tgctgtggct gaaaaccacg caaaccgtca ttgccgccca gttcaaccag cacccagcgc
    15361 ggctgatgct gtttcagcag agccggaagg cgcgccagtc cttgttgcga ggtgtcgccg
    15421 ctgatgctgg cattaactac cgacgtttta ctctgccact tatcattcaa caaggcaggc
    15481 caggccgcgc tggcagacat tcgatacccg gcgctcaggc tatcacccag aatcaataac
    15541 gtgtccgctg cggcggcacg gaaggttaac aggaccagga acaggaaggg caaatgccag
    15601 cggaaaacat tgttgaagtt catcatctta agaagtccgt cggtcagggg gagcatgaac
    15661 tctccatcct caccggagtt gagctggttg tcaaacgtgg cgagaccatc gcactggtgg
    15721 gcgagtcggg atcgggtaag tcaaccttgc tggcgatcct cgccgggctt gatgacggca
    15781 gcagtggcga agtgagtctg gtgggacaac cgctacataa tatggacgaa gaagcgcggg
    15841 caaagttgcg cgcgaagcac gtcggctttg tttttcagtc atttatgtta attcctaccc
    15901 ttaacgcgct ggaaaacgtc gagcttccgg ctctgctgcg cggtgagagt agcgcggaaa
    15961 gtcgtaacgg ggcgaaagcg ttgctcgaac agttagggct gggtaaacgt ctggatcatc
    16021 ttccggcaca gctttccggc ggtgaacagc aacgagtggc gctggcacga gcctttaatg
    16081 gtcgacctga tgtgctgttt gccgacgaac ccaccggcaa ccttgaccgc cagacgggcg
    16141 ataaaattgc cgacctgctg ttttccctca accgtgaaca tggcaccacg ttgattatgg
    16201 tgacccacga cctgcaactg gcggcacgct gcgaccgctg cttacggctg gtgaacgggc
    16261 agttgcagga ggaagcatga ttgcacgttg gttctggcgc gaatggcgtt cgccgtcgct
    16321 attaattgtc tggctggcgc taagcctggc ggtggcctgc gtgctggcgc tgggcaatat
    16381 cagcgatcgc atggagaagg gcttaagcca gcaaagccgt gagtttatgg cgggcgatcg
    16441 ggcgttgcgc agttcacgcg aagtgccgca agcgtggctg gaggaagcgc aaaagcgcgg
    16501 cctgaaagtc ggcaagcagc tgactttcgc cacaatgacc tttgcaggcg acacaccgca
    16561 gctggcgaac gtcaaagcgg tggatgatat ctacccgatg tatggcgatc tgcaaactaa
    16621 tccccctggc ctgaaaccgc aggcgggcag cgtattgctg gccccacgcc tgatggcact
    16681 gcttaacctg aaaacgggcg acaccattga cgtgggcgat gccaccttgc ggattgccgg
    16741 agaagtgatt caggaaccgg attccggttt taaccccttc cagatggctc cgcgtctgat
    16801 gatgaatctg gcggatgtcg ataaaaccgg agccgtgcag ccggggagtc gggtcacctg
    16861 gcgttataaa ttcggcggca acgagaacca gctcgacggc tatgagaaat ggttgttacc
    16921 tcagcttaaa cccgaacaac gctggtacgg tctggaacag gacgaaggcg cgctggggcg
    16981 atcgatggaa cgctcgcaac agttcctgct gctttcggcg cttctgacct tgctgctggc
    17041 agtggcagcg gtggcggtag cgatgaatca ttactgtcgc agtcgctacg atctggtggc
    17101 gatcctcaaa acgctggggg cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg
    17161 gttgatggtg ctgacgcttt cagccgttac cggtggggcc ataggcctgt tgttcgaaaa
    17221 cgtgttgatg gtgctgctca agcccgttct gcctgctgca ctaccgccag ccagcctctg
    17281 gccgtggctg tgggcgcttg gcaccatgac ggtcatctcg ctgctggtgg ggctacgacc
    17341 ataccgcttg ttgctggcaa cgcagccttt acgcgtatta cgtaatgatg tggtagcgaa
    17401 cgtctggccg ctgaagtttt atctgccgat tgtcagtatg gtggttgtgc tgctgctcgc
    17461 cggattaatg ggtggcagca tgctgctttg ggcggtgctg gcgggcgcgg tagtactggc
    17521 tttgctgtgc ggtgtgctgg gctggatgca gctgaatgta ctttgccgca tgacgctgaa
    17581 atcgctacct ctgcgcctgg cggttagccg cctgttacgt cagccgtggt caacgttaag
    17641 ccagctttcg gcattttcgc tctcctttat gctgctggcg ctgttgctgg tattgcgttg
    17701 cgatctgctc gaccgctggc agcagctacc gccagaaagc ccgaactact ttttaattaa
    17761 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    17821 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    17881 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    17941 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    18001 agaggggctg gcgaaacgct taaacgttgc cctcggtggt accgtgactt ttatgggcga
    18061 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    18121 gcctaatttc tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct
    18181 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    18241 gaccattagc ctgttagata ttggcgcgat tttaaaacag gtcggttagg tgctggagca
    18301 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    18361 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    18421 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    18481 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    18541 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    18601 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    18661 ggcgctgttc aggcagtttg cggggtgatg agagtaaata aggggcgttc tggttgaatg
    18721 gaacgccttg tttaaattaa ttctacaata tgtttcattg atgatgttat tgaattggtg
    18781 atttcctatc tttctattgc tgatattaat ctgaattggt ggagtttaat aattttaaat
    18841 atagccctac aaaaataact cagactaata aaatcataaa tcatatgcgt tgaatggata
    18901 ttatccatat agtgaatttg ttgatgatga attcatctgt gctaaagatt ttagtttaat
    18961 aaaatattga aagtgacctg taataacagt tattgttgat tgagaacaaa taagtttatg
    19021 tgaaaaatat ataaatacat tagctggtca tgtgtgtcat tttatttttt tgttgctaac
    19081 acagggatat gaacaataac taaaagggca ctttatatga gcggaaaacc agcggcgcgt
    19141 cagggagata tgactcagta tggcggtccc attgtccagg ggtcggcagg tgtaagaatt
    19201 ggcgcgccaa ctggcgtggc gtgctcggtg tgtccgggcg ggatgacctc gggcaacccg
    19261 gtgaacccgc tgctcggtgc aaaagtacag cccggcgaga cggaccttgc gctgcccgac
    19321 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcgcca
    19381 gtgggaactt tttggccccg gctggaaagc gccttctgat atccgcttac agatacgcga
    19441 tgatgcactg gtactcaacg acaacggcgg gcggagcatt cactttgagc cgctgctgcc
    19501 ggggaaggct gtgtacagcc gcagtgagtc aatgtggctg gtgcgtggcg gtaaggcagc
    19561 gcagccggac ggccacacgc tggcacgact gtgggcgtcg ctgccgccgg atatccggtt
    19621 aagcccgcat ctttacctgg cgaccaacag cgcacagggg ccgtggtgga tactggggtg
    19681 atctgagcgg gtgccgggtg ctgaggacgt actgccagcg ccgctgccgc cgtaccgggt
    19741 gcttaccggg atggcggacc gcttcgggcg gacgctgacg taccggcgtg aggccgcagg
    19801 cgacctggcc ggggaaatca ccggcgtgac ggacggtgcc gggcgggagt tccgtctggt
    19861 gctgaccaca caggcgcagc aggcggaaga ggcccggcag caggcggtcg tcgccggagc
    19921 aaaacgaccg gacattcctg attcactgcc ggagagcaca gagtacggcc ccgacagggg
    19981 tatccgcctt tcggcggtgt ggctgatgca cgacccggca tacccggaga gcctgcccgg
    20041 tgcgccactg gcgcggtaca cgtatacgga agccggtgaa ctgctggcgg tatatgaccg
    20101 cagcaatacg caggtgcgcg ctttcacgta tgacgcgcag cacccgggcc ggatggtggc
    20161 gcaccgttac gcgggaaggc cggagatgcg ctaccgctac gacgatacgg ggcgggtggt
    20221 ggagcaactg aacccggcag ggttaagcta ccgctatctt tatgagcagg accgcatcac
    20281 cgtcaccgac agcctgaacc ggcgtgaggt gctgcataca gaaggcgggg ccgggctgaa
    20341 acgggtggtg aaaaaagaac tggcggacgg cagcgtcacg cgcagcgggt atgacgcggc
    20401 aggaaggctc acggcgcaga cggacgcggc gggacggcgg acagagtacg gtctgaatgt
    20461 ggtgtccggc gatatcacgg acatcaccac accggacggg cgggagacga aattttacta
    20521 taacgacggg aaccagctga cggcggtggt gtacccggac gggctggaga gcagccggga
    20581 atatgatgaa ccgggcaggc tggtatcgga gacatcgcgc agcggggaga cagtacgcta
    20641 ccgctacgat gacgcgcaca gtgagttacc ggcgacgaca acggatgcga cgggcagcac
    20701 ccggcagatg acggcggttc accgcgagga aggtatcagc ctttaccgcc actatgacaa
    20761 ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt gaaacgcagt atgagtacaa
    20821 cgccgcaggc gacctgactg ccgttatcac cccggacggc aaccggagcg agacacagta
    20881 cgatgcgtgg ggaaaggcgg tcagcaccac gcagggcggg ctgacgcgca gtatggagta
    20941 cgatgctgcc ggacgtgtca tcagtctgac taacgagaac ggcagtcaca gcgacttcag
    21001 ctacgatgcg ctggaccggc tggtacagca gggcggcttt gacgggcgga cgcaacgtta
    21061 tcattatgac ctgaccggaa aactcacgca gagtgaggat gaggggcttg tcaccctctg
    21121 gcactacgat gcgtcggacc gcatcacgca ccggacggtg aacggcgacc cggcagagca
    21181 gtggcagtat gacggccacg gctggctgag agaaatcagc cacctgagcg aaggccaccg
    21241 tgtcgctgtc cactacggct acgacgataa gggccgcctg acgggcgaac ggcagacggt
    21301 ggagaacccg gagacggggg aactgctgtg gcagcatgag acgaaacacg cctacaacga
    21361 acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt ggctgaccta
    21421 cggcagcggt tacctggcgg gcatgaagct gggcgggacg ccgctggtcg agtatacgcg
    21481 ggacaggctg caccgtgaga cggtgcgcag cttcggcagc atggcaggca gtaatgccgc
    21541 atatgaactg accagcacat acacccccgc aggccagtta cagagccagc acctgaacag
    21601 cctggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc gcatcagcgg
    21661 tccgcgacag acgcgggaat acggctacag cgccacgggc aggctggaga gcgtgcgcac
    21721 cctcgcaccg gacctggaca tccgcatccc gtatgcgacc gacccggcgg gaaaccggct
    21781 gccggacccg gagctacacc cggacagcac gctcacggcg tggccggata accgcatcgc
    21841 agaggatgcg cactatgttt accactatga tgaatacggc agactgacgg agaagacgga
    21901 ccgcatcccg gcgggtgtga tacggacgga cgacgagcgg acccaccact accactacga
    21961 cagccagcac cgcctggtgt tccacacgcg gatacagcat ggcgagccac tggtcgagag
    22021 ccgctacctc tacgacccgc tgagccgccg gacggggaaa cgggtgtggc gtcgcgagcg
    22081 tgacctgacg gggtggatgt cgctgtcgcg taaaccggag gtgacgtggt acggatggga
    22141 cggcgaccgg ctgacgacgg tacagaccga caccacgcgt atccagacgg tataccagcc
    22201 ggggagcttc gcgccgctca tccgcatcga aacagacaac ggtgaacggg agaaagcgca
    22261 gtgccgcagc ctggcggaga agttacagca ggaagggagc gaagacggtc acggtgtggt
    22321 gttcccggct gaactggtgg ggctgctgga caggctggag ggggaaatcc gggcaaactg
    22381 cgtgagcagt gaaagccgcc agtggcttgc gcagtgcggg ctgacggtgg agcggctggc
    22441 ggcacagata gagccggttt atttgcctga gcgaaaaata catctttatc actgtgacca
    22501 ccggggcctg ccgctggcgc ttatcagtga agacggcaat acggcgtgga gcgcggaata
    22561 tgatgaatgg ggcaaccagc ttaatgagga gaacccgcat cacctgcacc agccgtaccg
    22621 gctgccgggg cagcagtatg ataaggagtc ggggctgtac tacaaccgga accggtacta
    22681 cgatccgttg caggggcggt atatcactca ggacccgata gggctggagg ggggatggag
    22741 tctgtatgcg tatccgctga atccggtgaa tggtattgat ccattagggt taagtcccgc
    22801 agatgtagcg ctaataagaa gaaaagatca actaaaccat caaagag
//
LOCUS       DAJVTY010000052        22770 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVTY010000052 DAJVTY010000000
VERSION     DAJVTY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.052"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..199)
                     /gene="pspE"
                     /locus_tag="OQQ66_001831"
     CDS             complement(<1..199)
                     /gene="pspE"
                     /locus_tag="OQQ66_001831"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1904791.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVY"
     gene            complement(274..495)
                     /gene="pspD"
                     /locus_tag="OQQ66_001832"
     CDS             complement(274..495)
                     /gene="pspD"
                     /locus_tag="OQQ66_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1904792.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(504..863)
                     /gene="pspC"
                     /locus_tag="OQQ66_001833"
     CDS             complement(504..863)
                     /gene="pspC"
                     /locus_tag="OQQ66_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1904793.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(863..1087)
                     /gene="pspB"
                     /locus_tag="OQQ66_001834"
     CDS             complement(863..1087)
                     /gene="pspB"
                     /locus_tag="OQQ66_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1904794.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1141..1809)
                     /gene="pspA"
                     /locus_tag="OQQ66_001835"
     CDS             complement(1141..1809)
                     /gene="pspA"
                     /locus_tag="OQQ66_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1904795.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            1976..2953
                     /gene="pspF"
                     /locus_tag="OQQ66_001836"
     CDS             1976..2953
                     /gene="pspF"
                     /locus_tag="OQQ66_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1904796.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3073..4338)
                     /gene="puuE"
                     /locus_tag="OQQ66_001837"
     CDS             complement(3073..4338)
                     /gene="puuE"
                     /locus_tag="OQQ66_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1904797.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4376..5645)
                     /gene="puuB"
                     /locus_tag="OQQ66_001838"
                     /pseudo
     CDS             complement(4376..5645)
                     /gene="puuB"
                     /locus_tag="OQQ66_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(5647..7134)
                     /gene="puuC"
                     /locus_tag="OQQ66_001839"
                     /pseudo
     CDS             complement(5647..7134)
                     /gene="puuC"
                     /locus_tag="OQQ66_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(7409..7966)
                     /gene="puuR"
                     /locus_tag="OQQ66_001840"
     CDS             complement(7409..7966)
                     /gene="puuR"
                     /locus_tag="OQQ66_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1904798.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(7993..8756)
                     /gene="puuD"
                     /locus_tag="OQQ66_001841"
                     /pseudo
     CDS             complement(7993..8756)
                     /gene="puuD"
                     /locus_tag="OQQ66_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            8968..10386
                     /gene="puuA"
                     /locus_tag="OQQ66_001842"
     CDS             8968..10386
                     /gene="puuA"
                     /locus_tag="OQQ66_001842"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1904799.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLALNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10535..10700
                     /locus_tag="OQQ66_001843"
                     /pseudo
     CDS             10535..10700
                     /locus_tag="OQQ66_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10690..12075
                     /gene="puuP"
                     /locus_tag="OQQ66_001844"
     CDS             10690..12075
                     /gene="puuP"
                     /locus_tag="OQQ66_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1904800.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12209..12454
                     /locus_tag="OQQ66_001845"
     CDS             12209..12454
                     /locus_tag="OQQ66_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1904801.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            12767..14410
                     /gene="sapA"
                     /locus_tag="OQQ66_001846"
     CDS             12767..14410
                     /gene="sapA"
                     /locus_tag="OQQ66_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1904802.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14407..15372
                     /gene="sapB"
                     /locus_tag="OQQ66_001847"
     CDS             14407..15372
                     /gene="sapB"
                     /locus_tag="OQQ66_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1904803.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15359..16249
                     /gene="sapC"
                     /locus_tag="OQQ66_001848"
     CDS             15359..16249
                     /gene="sapC"
                     /locus_tag="OQQ66_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1904804.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16249..17241
                     /gene="sapD"
                     /locus_tag="OQQ66_001849"
     CDS             16249..17241
                     /gene="sapD"
                     /locus_tag="OQQ66_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1904805.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVIADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17243..18049
                     /gene="sapF"
                     /locus_tag="OQQ66_001850"
     CDS             17243..18049
                     /gene="sapF"
                     /locus_tag="OQQ66_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1904806.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18117..18470
                     /locus_tag="OQQ66_001851"
     CDS             18117..18470
                     /locus_tag="OQQ66_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1904807.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            18838..19626
                     /gene="fabI"
                     /locus_tag="OQQ66_001852"
     CDS             18838..19626
                     /gene="fabI"
                     /locus_tag="OQQ66_001852"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1904808.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            19771..20898
                     /locus_tag="OQQ66_001853"
     CDS             19771..20898
                     /locus_tag="OQQ66_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1904809.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            20966..>22770
                     /gene="rnb"
                     /locus_tag="OQQ66_001854"
     CDS             20966..>22770
                     /gene="rnb"
                     /locus_tag="OQQ66_001854"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1904810.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELV"
BASE COUNT         5111 a         6046 c         5961 g         5652 t
ORIGIN      
        1 aatacacttt cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt
       61 ccttcagggg aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac
      121 gaacatcgat ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa
      181 gtaagccttt tttaaacatg taagcgtcct tctatggatg aaaattgcca tccattatac
      241 gcctgagatt taacaatttt tgcgaaactc ccctcacctt ttataacgct gtgccagttt
      301 attagcagcc cgacttaaca gcggttccag cgccacagcc agcaacattt tcagcggtcg
      361 gcgagcaact gattttatcg cccagcccgc tacacccgcc gggccatagc gcagtgcggt
      421 aagaagtacc agcttgcctg ctaatttgaa accaggcttt accttttgcc cggcctgttg
      481 ccagcgagta ttcataactt tcctcacagt tgacggaaac ggctacgtaa cgtgaaagta
      541 tcggaagtga cataacgttc catctcgcgt aaacgcgttt cacttgccgc cagttcacgg
      601 tcgacttcat ccagcaattc gctgctggag ggtagctgct caccaaaggc catgttgtcc
      661 ggcattggat caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa cagcgccaga
      721 ccgaagaaaa tcgacagcac caccaggata cgcaccagtt ttaccggtac atcaaaatag
      781 ttggcaatcc cggcgcagac gccgcggacc atgccctgct gtggaatacg ccataatttt
      841 ttattgagat taatgcccgc cattagcgat ccctccagtt cggatgttct gcatcaagaa
      901 tagattccag cgcctgaata cgttcgcgca tccgttttgc ttcatcagcc agttgcgcta
      961 atcgctgctg ctcactttgc gacaattcac tgcgaccaga acgattgctg taatgcagcc
     1021 ataaccagat cggtaaaaca aacagcacaa aaatggttaa cggaatagcc agaaatagcg
     1081 cgctcataag tactccttag acgatgagcg gcggcgcgtt cggacgccgc ctagatgtta
     1141 ttattgattg tcttgcttca ttttggcttt taattgtgcc agttgttcgc tgattgcatc
     1201 atcggctttc agttcggcaa actgatcgtc cagcgatttt tgtttaccga agctgtggct
     1261 ttctgcttcc gcttccatct ggtcaatacg acgttcgaaa gattcgaaac gagccattgc
     1321 ttcatccagt ttgccactgt ccagctgacg acgcacatcg cgcgacgagt ttgccgcctg
     1381 atggcgtaac atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt tttccagctc
     1441 accgctctct ttcttcatgc gtgccagcgt atcgtccacc agcgtcactt catgttccag
     1501 actcttaatc agatcggtca gtttctgttt ttcaattaac gccgcacgtg ccaaatcctc
     1561 tctctctttc agcagcgcca gttcggcttt ttcctgccat tcaacttcac gcgccgacgc
     1621 ttgttcaata cggcgagtca gctgtttctt ttctgccaac gcacgcgccg aagtagaacg
     1681 tacttcaacc agtgtatctt ccatctcctg gatcatcaga cgcaccagtt tctgtggatc
     1741 ttccgctttc tctaacagag cgttgatgtt ggcattcacg atgtcggcaa agcgagaaaa
     1801 aatacccata atgttgtcct cttgatttct gcgttcagga ttgtcctgct gaactgttaa
     1861 tacaatttgc gtgccaattt tttatctttt tgatttataa agatctgatt gaagaatcat
     1921 cagcaacatg ccaggatgaa ttagcgaatt acactaacaa gtggcgaatt tcatcatggc
     1981 agaatacaaa gataatttac ttggtgaggc gaacagcttt ctcgaagtgc tggaacaggt
     2041 ttcgcatctc gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg gcaccggtaa
     2101 agagctgatt gccagccgcc tgcattatct ctcctcccgt tggcaagggc cgtttatttc
     2161 ccttaactgc gcggcgttaa atgaaaatct gctggattcc gaactgtttg gtcacgaagc
     2221 gggggcgttt accggtgcgc aaaaacgtca tccagggaga tttgaacgtg ccgacggcgg
     2281 tacgctattt cttgatgaac tcgctacggc accgatgatg gtgcaggaga aattattgcg
     2341 cgtgattgag tacggtgaac tggagcgcgt tggcggcagc cagccattgc aggtgaatgt
     2401 gcggttggta tgcgcgacga atgccgatct cccggcgacg gtcaatgaag gcacttttcg
     2461 cgctgacctg ctcgaccgac tggcttttga tgttgtacaa ctgccaccac tgcgcgagcg
     2521 cgaaagcgac ataatgttga tggcagaaca ctttgccatc cagatgtgtc gggaaatcaa
     2581 gctgcctctg ttcccggggt ttacggagcg cgccagagaa acattgctga attatcgttg
     2641 gccgggaaat attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc gccacggcac
     2701 cagcgattat ccgcttgatg acatcattat tgatcccttt aaacggcgtc cgcctgaaga
     2761 cgctatcgcc gtttcagaaa ccacctcgct tccaacactg ccgctggatt tacgtgagtt
     2821 tcagatgcag caggaaaaag agttgctgca actcagtttg caacaaggga aatataacca
     2881 gaagcgcgcg gctgaattac tggggttaac ttatcatcag ttccgcgcgt tgttgaaaaa
     2941 gcaccagatt tagcgaagtt gagcaataca acttgccgga tgcggcgtga atgctttatt
     3001 cggcatacat aatcaataag tcgtttgcag gcctgataag cgcagcgcat cagacattat
     3061 tgcgttgggc tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg
     3121 cgcatccgga atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg cgccacaggt
     3181 cagcaggagc agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga
     3241 cggctcgccc gtttgcggat cgttaaactc tgccgcaatc atcgacccca ggccgcgtac
     3301 cgcagcaatg gccggaacgc tttctttggc atcaatcaac gtgtttttga gacgctggcc
     3361 cagttgattc gcgcgttcgc agagtgattc tttgtcgata atattgagca ccgcgtgcgc
     3421 ggcagccacc gccagcgggt tcccggcgta ggtgccaccc aacccgcccg gcgcgggcgc
     3481 gtccataata ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg cgaggctttt
     3541 cgccatcgtc attaagtccg gcttatcggc gtaatgatcc atggcaaaca gtttaccggt
     3601 acgcgcaaag ccgctttgca cttcatcggc aatcatcaca ataccgtgct cgtcgcacag
     3661 gcggcgaatg gcggcaacca gctcttttgg cgcaacgttg aaaccgccct cgccctgcac
     3721 cggttcgaaa ataatggccg ccacctgctt cgcttcaatg tccgatttaa acaagcgttc
     3781 gatggcgtcg agggagtcct gtgttgaaat gccgtgtaaa tctgacggat aaggtacgtg
     3841 atacaccgaa ccagggaacg ggccgaagcc gattttgtac ggcgcaactt ttccggtcag
     3901 cgccatggtc atatatgtac gaccgtggaa gccgccgcta aacgcaatca cgccaggtcg
     3961 tccggtatgg gcgcgagcaa ttttcaccgc gttttccacc gcttccgcgc cggtagtgaa
     4021 gaatgcggtt ttagcctgcc cgctcactgg ggcaagggcg ttaattttct ccgccagcgt
     4081 gacgtagctt tcgtacggca caatctgata cgccgtgtgg gtaaactgtt gcaattgctg
     4141 ctccaccgcc gcgaccagat cagggtggcg atgcccggta ttcagcaccg caatgcctgc
     4201 ggcgaaatcg atgtattcgt tgccctctac gtccttcagc gtagcgtttt ctgccgactg
     4261 ggcgaagaag ttacacatca cgccaacccc gcgtggggta gcagaaagac gacgctgatg
     4321 gaattcattg ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa
     4381 cgcccagacg atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaat ggcacgcgca
     4441 gcgtgcgccc gccggggaac gggtaatgcg gcagattggc gaaggcatcg aaacgctcgg
     4501 cgtcaccgcg cagcagttca gcaatcaaac gcccggcgag gtgggtacag gtgacgccgt
     4561 ggccgctgta gccctgcatg taatagatgt tggtatcgag gcgaccgaac tgcggcatcc
     4621 gtgaaagggt cagcaggaag ttgcccgtcc agcgatagtc gattttgacg cccttcagct
     4681 gcgggaaggt tttcagcagt ttcggcacaa caagacgctc aacgtcatcc gggtcgcgcg
     4741 cgccgtagac cacgccgccg ccgtacagca aacggttgtc ggcggtaaga cggtagtaat
     4801 caagcagata attacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat
     4861 cttccgacag ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg cgtttcgcca
     4921 gttccggctc tactttatcg cccagatacg cattcccggc gacaatcaca tacttcgccg
     4981 tcacctgacc tttggcagtg cgcacaacgg ctggcgtggt gtgctggatc tgcgtcacgg
     5041 cagaaagttc atacacgcgc ccgccgttga ggcggatggc gtccgcttcg ccaatggcga
     5101 ggtttagtgg gtgaatatgc ccaccgctgt gatccagcag cgcaccggta tagcgatcgc
     5161 tggctacttc acggcgaatg gcgttggcgt ccagcaattc cagctgttta ttgccgtagc
     5221 gttcccagtt ctctttctgc tcttcaagtg tggcgagctg tttatcattc atcgccacaa
     5281 acaggctgcc ggggcggtag tcgcagtcaa tctgataacg tttgatacgt tcgcggatga
     5341 tctcaccacc ttcgaacatc atgctgccga gcataggggc ggtgtccatg ccgtagcttt
     5401 tttcgatcac gtcgatgtcg cggttgtagg agttcacaag ctgcccgcca ttgcgcccgc
     5461 ttgcgccaaa gccgatgcgt gaggcttcga gaaccactac gtcaaagccc gcttccgcca
     5521 gatgcagcgc ggaggagagc ccggtatagc cgccgccaac cacgcaaacg tcgcaggtga
     5581 ttgactcatt cagggtgtcg aatggcacgt atttatgcgt aataactgct ggtatgttcg
     5641 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcggtgaatt tttcaagggc
     5701 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     5761 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcggc ggctcatacg
     5821 gtgcgcgcgg gagaggtcgc gcatccatac cgccgcgcca aggccgtact ggctgtcgtt
     5881 ggcaagctgt agcgcctgtt cttctgatgt gaaacgcgtg accaccagca ccggaccgaa
     5941 aatctcttcg cggcttaagg acgcattcgg gtctacatcc acaaagatgg tcgggccgat
     6001 ggcggcagcc agcccggcgt tacggccatc cagcagcagt tgccctttgc cttcgccctc
     6061 acgaataaag ctatggaccg agtcggcgtg ggcgcagtcg attaaagtgc ccatggtggt
     6121 tgcggggtca agcggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     6181 gaattcatcg gcgatgctct cttccagcaa caggcgtgtt ccggcgatgc acacctgacc
     6241 ctggttgtag aagatgcctg ctgcggtgga gctcgccgct ttttgcagat ccgaacaatc
     6301 ggagaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct
     6361 gtcgcccgca tctttcagca gctgtttccc ggtacgggtt gaaccggtaa aggcaatggc
     6421 gtcgatatcg ttatgacgcg acagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     6481 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccacgagac gaatcgcact
     6541 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     6601 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     6661 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     6721 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     6781 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     6841 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     6901 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     6961 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgactg gatcaacggt
     7021 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     7081 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     7141 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     7201 ggccgtatgc ccgtttattt ctggcgacat tctttatata cacttcatcc ttcaagccgc
     7261 ctctgccttg gctgcgttcg ctcccccgaa tcacttacct gagtaagctc atcgggattc
     7321 gctcacttgc cgccttgatg caacttgaat gattttgtgt atagattgcc gggcgcattg
     7381 cgcttacccg gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa
     7441 ttcggcaaat acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat
     7501 aactttgacc cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt
     7561 ccagtactgt gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg
     7621 gctggtacgt ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct
     7681 tcattgacac accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg
     7741 gctcatcagg tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca
     7801 gcagcttttg cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag
     7861 cactgtgagt cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc
     7921 ggatttccga caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt
     7981 aaactgtagt ggtcagagtc gctgtttttc agcgatatgg tgctgacaag cggtgataaa
     8041 gccctcgaac aatatacgcg aaagagcgta ctcgctactg ttccattccg ggtgccactg
     8101 tacgcccagc gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga
     8161 gcgagcttca acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt
     8221 tacccaaaag ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg
     8281 aacttcatgc gacggtgcat attgctgttc caccggcagt tcggatcttc ccgatgttcc
     8341 agcaattcag gctgttcgca cagcttgcga tgcaacgaac caccggttgc caccaccagt
     8401 tcttgtaaac cccggcagat ggcgaaaatg gggatgcgcc tttcgagtgc ggcattaatt
     8461 atcgccatgc tcagaagatc acgcccggga tcggcgtcag gctcatcgcc gttttcacca
     8521 tataggtgcg gctgcacatt gctgggacta ccaggaagat aaatgccatc gagtttcggc
     8581 aaaagttgtt caagtaacga cggttccgcc agcgcatgtg gtagcgcaat tggcaagccg
     8641 cctgcgtgga tgatggcatt caggtacttt tcttgcagag tctgggtcgc atgaccctta
     8701 agcctgttcc tgcacattac gacaccgata accggattgt tcattatatt tttcatgctc
     8761 aatctcacaa agtggactaa attatcgcca ttactgcgct ttaaatgacc tttatgttca
     8821 atattttttc aatctagcag tgggttttca tttttgcaaa ctaaatttaa catttggcaa
     8881 acatttagtt tgcatacaga ttcgaatggt ggtcattata ttttacgctt tgataacgag
     8941 cggaaaacaa accaaaggcg aagaatcatg gaaaccaata tcgttgaagt agagaacttt
     9001 gttcagcagc cagaagagag gcggggtagc gcctttacgc aggaagtgaa acgctacctg
     9061 gagcgctacc cgaatacgca atatgttgat gttttactta ctgatttgaa tggttgcttc
     9121 cgtggaaaac gtatccctgt ttcaagcctg aagaagttgg agaaagggtg ttatttcccg
     9181 gcctcggtgt ttgcaatgga tattctgggc aacgtagtag aagaagcggg tctgggtcag
     9241 gaaatgggcg agccggatcg tacttgtgtt cctgttctcg gttccttaac cccttctgcc
     9301 gccgatccag agtttatcgg tcagatgctc ctgaccatgg tcgatgaaga tggcgctccc
     9361 tttgacgttg agccgcggaa cgttcttaac cgcctgtggc agcagctacg ccagcgcgga
     9421 ttgttcccgg ttgtagcggt agagctggag ttctatttac tggatcgtca gcgcgacgct
     9481 gaagggtatc tgcaaccgcc ctgcgcgccc ggcaccgata accgcaatac acaaagccag
     9541 gtttactccg ttgataacct caaccacttc gccgacgtgc tcaatgatat tgatgaactg
     9601 gcgcagttac agttaattcc cgccgacggt gcggtcgccg aagcctcgcc gggtcagttt
     9661 gaaatcaacc tttaccatac cgataacgtg ctggaagcct gcgatgatgc gctggcgcta
     9721 aaactcctgg tgcgtctgat ggcagaaaag cataagatgc actccacttt tatggcgaag
     9781 ccgtatgaag agcacgcggg cagcgggatg catatccata tcagtatgca aaataatcgt
     9841 ggcgagaatg tgctttctga cacagaaggc gaagattcgc cgctgctgaa aaagatgctc
     9901 gccgggatga ttgacctgat gccgtcgtcg atggcgttgc tggcactaaa cgtgaactcg
     9961 tatcgccgct tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg ccataacaac
    10021 cgcaccgtcg ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt ggaatatcgc
    10081 gtggcgggtg ccgatgccaa cccatatctg gtgatggcag cgatttttgc cggtattttg
    10141 catggccttg ataacgagct gccgttgcag gaagaagtcg aaggcaacgg gctggaacag
    10201 gaaggcttac ccttcccgat tcgccagagc gatgccctgg gtgagtttat cgagaatgat
    10261 cacctgcgcc gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat
    10321 gagctgttgc agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac
    10381 gcatagtacc ccttgcgggg ttgtttcatc gacctgagcg gagccaggcc tgacatttgc
    10441 tggtaatcca gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg
    10501 ttaacgatta agactgtgac gaggaaatga gatgatgccg atgattaaat ccccgcatgg
    10561 cgagggaggc tgcgtatgcg cccctcctgc tactgactgg actccgtccc ctttgcttcc
    10621 cttgctaaac aggtttgatt tcaggtcgac acgaccgcaa accttattac gcagggggag
    10681 gcagcaatta tggctattaa ttcaccactg aatattgctg cgcaacccgg caaaacccgt
    10741 ctgcgaaaat cactgaaatt gtggcaggtg gtgatgatgg gtctggccta tctcacgccg
    10801 atgaccgtat ttgatacttt tggtattgtc tccggcatta gcgacggtca cgttccggcg
    10861 tcctatttgc tggcgctggc gggcgtgctg tttaccgcta tcagctacgg caaactggtt
    10921 cgccagtttc cggaggccgg ttcggcctat acctacgcgc aaaagtcgat taacccgcac
    10981 gtcggattta tggtcggctg gtcatcgctg ctggattatc tctttttgcc gatgatcaac
    11041 gtcttgttgg cgaaaatcta tctctccgcc ctcttcccgg aagtgccgcc gtgggtgtgg
    11101 gtagtaacct tcgtcgccat tttaaccgcc gcgaatctga agagcatcaa cctggtcgct
    11161 aacttcaata ccctgtttgt actggtgcaa atctccatca tggtggtgtt tatcttcctg
    11221 gtagttcagg gactgcataa aggagaaggc gttggcaccg tctggtcact tcagccgttt
    11281 atcagcgaga acgcgcacct gatcccgatt attaccgggg cgacgattgt ctgtttctcc
    11341 ttcctcggtt tcgatgcggt gaccacgctt tcggaagaga cgccggacgc cgcacgcgtg
    11401 atcccgaaag ccatcttcct gacggcggtc tacggtggcg ttatctttat cgcggcgtcg
    11461 ttctttatgc agctgttctt tcccgatatc agccgcttta aagatccgga tgccgcactg
    11521 cctgaaattg cgctgtacgt cggcggcaag ctcttccagt cgattttcct ctgcaccacg
    11581 tttgtgaaca cgttagcctc tggcctggct tcacatgcca gcgtgtcgcg tctgctgtat
    11641 gtgatggggc gtgacaatgt gtttccggag cgcgtgtttg gctatgtgca cccaaaatgg
    11701 cggactccgg cactcaacgt cattatggtc gggattgtcg cgttgtcggc gctgttcttc
    11761 gatttagtta ccgccacagc gttaattaac ttcggtgctc tggtggcgtt taccttcgtg
    11821 aatctgtcgg tgtttaatca tttctggcgg cgtaaaggaa tgaataaaag ctggaaggat
    11881 cacttccact atttgctgat gccgctggtt ggcgcgctga cggtgggtgt gctgtgggtt
    11941 aacctcgagt caacgtcact gacactcggt ctggtatggg cttcgttagg cggcgcatat
    12001 ttatggtatc tgatccgccg ctatcgcaaa gtgccgttgt atgatggtga cagaacgccg
    12061 gtgagtgaaa cgtaatagtc ggatgcgcgg cgcattatcc tgccggaggg taatgcaccg
    12121 cgccattaac tataaaaaga tgtatgaccc ggatcacgag tgctatcatc cgcccttatg
    12181 cttagcgcat taaccccagg ggcaaataat gaaccacgat atcccgctaa aatattttga
    12241 tattgccgat gagtacgcga ccgaatgcgc agagcctgtc gcagacgctg aacgcacacc
    12301 gctggcccac tacttccagt tactgctcac ccgtttgatg aacaatgaag agatcagcga
    12361 agaagcccaa catgaaatgg ctgctgaagc ggggattaat cccgtacgca ttgatgagat
    12421 cgcagagttt ttgaatcaat ggggtaatga gtaattattt aattgcagac gatcggtcag
    12481 tatcgttgtc cggataaagc gttcacgccg catccggcta acaatgacac gcactgcagc
    12541 gtgctttaat gggtttctca cgatcgtacc gctctgctcc tcgctaagtt gttctgtgag
    12601 ttatagttat agaggaagca cgggtacgaa tgttgaacag atggcgacgc cacctgttca
    12661 acgaccaacc acacggccct cattacgacc cctgaacaca aacggtaatg gcaaatgtca
    12721 ttaaatatta gtaagatagc ctctttacat aaaatccctt aatattatgc gccaggtatt
    12781 atcgtctctt ttggtgattg ctggacttgt gagtggtcag gcaatcgccg cgcctgaatc
    12841 ccccccgcat gctgatatcc gcgacagcgg ttttgtctat tgcgtcagcg ggcaagtcaa
    12901 cacctttaac ccatccaaag cgagcagtgg gttaattgtc gatacccttg ccgcccagtt
    12961 ttatgatcga ctgctggatg tcgatcccta tacctatcgc ctgatgccgg aacttgccga
    13021 aagctgggaa gtactcgaca acggcgcgac ctatcgcttc cacctgcgtc gcgatgttcc
    13081 gtttcaaaaa accgactggt ttactcccac tcgtaaaatg aatgccgacg atgtggtgtt
    13141 taccttccag cgaatttttg accgcaacaa cccgtggcat aacgtcaacg gcagcaactt
    13201 cccctacttc gacagcctgc aatttgccga taacgtgaaa agcgtccgca aactggataa
    13261 tcataccgtt gagttccgac tcgcccagcc ggatgcttct tttttgtggc acctcgcaac
    13321 ccattatgct tcggtcatgt cggcagaata tgcccggaag ttagagaaag aagatcgcca
    13381 ggagcaactc gaccgtcaac cggtcggcac cggtccgtat cagttgtcgg aataccgcgc
    13441 cgggcaattt attcgcctac aacgtcatga tgacttctgg cgcggtaaac cgttaatgcc
    13501 gcaggtagtg gtggatttag gctccggcgg caccggacgt ctgtcgaaac tcctgaccgg
    13561 ggaatgcgac gttctggcct ggcctgctgc cagccagcta tccattttgc gtgacgaccc
    13621 gcgcttgcgt ttaacgctgc gtcctgggat gaacgtcgcc tatctggcat ttaacaccgc
    13681 caaaccgccg ctaaataatc ccgctgtccg ccatgcactg gcactggcga ttaataacca
    13741 gcgcctgatg caatccatct attatggtac ggctgaaacg gcggcctcta ttttaccgcg
    13801 cgcctcgtgg gcctatgaca acgaggctaa aattactgaa tacaatccgg cgaaatcgcg
    13861 cgaacagttg aaggcgttgg ggctggaaaa cttaacgctg aaactgtggg tgcccacacg
    13921 ttcgcaggcg tggaacccca gtccactgaa aactgccgaa ctgattcagg cggatatggc
    13981 gcaggttggc gtaaaagtgg tgattgtgcc ggtagaaggt cgctttcagg aggcgcggtt
    14041 gatggatatg agccatgatc tgacgttatc cggttgggcg acggacagta acgacccgga
    14101 cagtttcttc cgtcctttac tgagctgcgc ggcaattcat tcacagacca acctcgccca
    14161 ctggtgcgat ccgaaattcg acagcgtgtt gcgtaaggcg ctctcctcgc agcagctggc
    14221 ggcgcgtatt gaagcctatg acgaagcgca gagtattctg gcgcaggaat tgcccatttt
    14281 gccgctggcg tcgtcattgc gtttgcaggc ctatcggtac gatatcaaag gtctggtact
    14341 tagcccgttt ggtaacgcct cctttgctgg ggtgtatcgc gagaaacagg atgaggtgaa
    14401 aaaaccatga ttatcttcac cttacgccgc attttgttat tgattgtcac cttgttcctg
    14461 ctgaccttcg ttggcttcag cttgagctat ttcacccctc atgcgccgtt acaaggcgcg
    14521 tcactgtgga atgcctgggt gttctggttt aacggcctga tccactggga ttttggtgtt
    14581 tccagcatta acggtcaacc gattgccgaa cagttaaaag aggtcttccc ggcgacgatg
    14641 gaactgtgca tcctcgcctt cggttttgcg ctgattgttg gcatccccgt ggggatgatt
    14701 gccgggatta cgcgccataa gtggcaggat aacttgatca acgccatcgc cttactgggc
    14761 ttttcaatcc cggtgttctg gctggcgctt ctgttgacgc tgttttgttc actcacgcta
    14821 ggttggttgc ccgtttcagg gcgtttcgat ctgctctacg aagtgaaacc gattaccggt
    14881 tttgcgttga ttgatgcctg gctttcggac tcaccatggc gggatgaaat gatcatgagc
    14941 gcaatccgcc atatgatatt gcccgtgatc accctgtcgg ttgcgccaac aactgaagtg
    15001 atccgcctga tgcgtatcag cactatcgaa gtgtatgacc agaactatgt aaaagcggcg
    15061 gcgacccgcg gtttgtcgcg ctttactatt ttgcgtcgcc acgttctgca taacgcgtta
    15121 ccgccggtta ttcctcgcct gggcttacag ttttccacca tgctgacgct ggcgatgatc
    15181 accgaaatgg tttttagctg gccgggttta gggcgctggt taattaacgc catccgccag
    15241 caggattacg cagccatttc cgccggagtg atggtgtgtg gctcactggt cattattgtt
    15301 aacgtgattt ctgatatttt gggtgccatg gctaaccctc tgaaacataa ggaatggtat
    15361 gccttacgat agcgtataca gcgaaaagcg cccgccgggc acgctgcgta ccgcctggcg
    15421 caaattttat agtgacgcct ctgcgatggt cggcctgtac ggctgcgcgg ggctggctgt
    15481 actgtgtatt tttggcggct ggtttgcgcc ttacggcatc gaccagcaat ttctcggtta
    15541 tcaattattg ccgccatcat ggtcgcgcta tggcgaagtt tctttcttcc tggggactga
    15601 cgacctcgga cgcgatgttt taagccgttt actgagcgga gctgcgccaa ccgtgggtgg
    15661 cgcatttgtg gtgacgcttg ccgcgacgat ctgtggccta gtactgggca ccttcgccgg
    15721 ggcgacacac ggcctccgct cagcggtgct taaccatatt ctggatacct tactggcgat
    15781 cccttcgctg ctgttggcaa ttatcgttgt agcgtttgcc ggaccgagtt tgtcccacgc
    15841 tatgtttgct gtctggctgg cattgctgcc gcgtatggta cgttcgattt acagcatggt
    15901 gcatgacgaa ctggaaaaag agtacgttat cgccgcccgt ctggatggcg catcaacgct
    15961 gaatattctc tggtttgctg tgatgccaaa catcaccgcc gggctggtca ccgagatcac
    16021 ccgcgcactg tcgatggcaa ttctcgatat cgccgcgctg ggctttctcg atctcggcgc
    16081 acaactcccc tcgcctgaat ggggagcgat gctcggtgat gcgctggaac tgatttatgt
    16141 cgccccgtgg actgtcatgc tgccaggtgc ggcaattatg attagcgtgt tgttggttaa
    16201 cctccttggc gatggcgttc gtcgtgcgat tattgcgggg gtggaataat gccattactc
    16261 gatattcgta acctgaccat tgaatttaaa accggtgatg agtgggttaa agccgtcgac
    16321 cgggtaagta tgaccttaac cgaaggtgaa atccgcggtc ttgttggtga atccggttcc
    16381 ggcaagagtt tgattgcgaa agcgatttgt ggggtgaata aagataactg gcgtgttatt
    16441 gctgaccgta tgcgttttga tgatatcgat ttgctgcgtc tctccgcacg cgaacggcgc
    16501 aaactggttg gccataacgt gtcgatgatt ttccaggaac cgcagtcgtg tcttgaccct
    16561 tcagaacgtg tgggtcgcca gttgatgcaa aacatcccag cctggaccta taaaggccgt
    16621 tggtggcagc gttttggctg gcgcaaacgc cgtgccattg aactgctgca ccgcgtgggg
    16681 attaaagatc acaaagatgc gatgcgcagt tttccctatg agttgaccga aggtgaatgt
    16741 cagaaagtga tgatagccat tgcactggcg aatcaaccgc gtctgctgat tgctgacgaa
    16801 ccgaccaact caatggagcc aacaacccag gcgcaaatct ttcgcctgct gacgcgtctc
    16861 aaccaaaaca gcaataccac tattttgctt atcagccatg acttacaaat gcttagccaa
    16921 tgggcggata aaattaacgt gctttactgc ggtcaaacag tggaaaccgc gccgagtaag
    16981 gagttggtga cgatgccaca tcatccttat acccaggcgc tgatccgcgc gataccagac
    17041 ttcggcagcg cgatgccgca taaaagtcgc ctcaatacgc tgcccggcgc tatcccactg
    17101 ctggaacagt taccgattgg ttgtcgtctg gggccacgtt gcccgtatgc acaacgagaa
    17161 tgcattgtga cgccacgttt gacgggggcg aaaaatcatc tctatgcctg tcatttcccg
    17221 ctgaacatgg agaaagagtg agatgatcga aacgctgctt gaagtgcgta atttaagtaa
    17281 aacgttccgc taccggaccg gctggtttcg ccgtcagacc gtagaagcgg taaaaccctt
    17341 gagctttacg ctacgtgaag gccagacact ggcgattatt ggcgagaatg gttcgggtaa
    17401 atccacgctg gcaaaaatgc tggcgggaat gatagagcct accagcggcg agttattgat
    17461 tgacgatcat ccactgcatt ttggcgatta ttccttccgc agtcagcgta ttcgcatgat
    17521 ttttcaggat ccttcgacct cgttgaatcc ccgtcaacgt atctcgcaaa ttctggattt
    17581 tccactgcgc ctgaacaccg acctggaacc ggaacagcgt cgcaaacaga ttattgaaac
    17641 gatgcgtatg gttgggctgc taccggatca cgtcagttac tatccgcata tgttggcacc
    17701 cggacaaaaa cagcgtctgg gtctggcgcg cgcgctgata ttgcgcccaa aagtcattat
    17761 tgccgatgaa gcactcgctt cactggatat gtcgatgcgt tcgcagttga ttaatctgat
    17821 gctggagtta caggaaaaac agggcatttc gtatatttat gttacccagc atatcggaat
    17881 gatgaagcac attagcgacc aggtgctggt gatgcatcag ggcgaggttg tcgagcgagg
    17941 cagcaccgcg gatgtgctgg catcgccgct gcatgaactc accaaacggc tgattgccgg
    18001 gcactttggc gaagcattga cagcggatgc gtggcgtaaa gatcgctaat tttctgaatc
    18061 ctccttccag attatccacc tcattctgac aagagattaa atcttgtcag aatgaggtga
    18121 tggataaaat aaaatcaaat gcgcgtgatt taagacgcaa tctcactttg caggaacgaa
    18181 agctctggcg atatcttcgc agccgacgtt ttggtgattt caaatttcgc cgtcaacatc
    18241 cggtggggag ctacattctc gattttgctt gctgctcggc gcgcgtagtc gttgagctgg
    18301 atggtgggca gcatgatttg gcagttgcct atgataccag gcgcactagc tggcttgagt
    18361 cgcagggctg gaccgtgctg cgtttctgga ataacgagat tgattgtaat gaggaggcgg
    18421 tgctggagat tattctgcag gaactgaacc gccgatcacc ctcaccctga aagggcgagg
    18481 gggccgaccg agccgaatag ctgttgtggt gaaaacatgg agactgtgct ggagaatatt
    18541 cggcaaggtc tgaaccatcc cagccatcgc cataaaaggg ttaggggctg tatgagcctg
    18601 tttgttgctg gggtaacaat atttgcacaa tatggtcccc tcgcccctct ggggagaggg
    18661 ttagtgtaag gggaaaagcg cccaccctgc cgcagcctgc tccggtcgga cctagcaact
    18721 atagctactc acagccaggt tgattataat aaccatttat ctgttcgtac tgtttactaa
    18781 aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg
    18841 ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact atccatcgcc
    18901 tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac
    18961 gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga catcgttctg
    19021 cagtgcgatg ttgcagaaga tgccagcatc gacaccatgt tcgctgaact ggggaaagtt
    19081 tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat
    19141 ggtgactatg ttaacgccgt tacccgtgaa ggcttcaaaa ttgcccacga catcagctcc
    19201 tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg
    19261 ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg
    19321 gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt
    19381 gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcttc cggtatcaaa
    19441 gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact
    19501 attgaagatg tgggtaactc cgcggcattc ctgtgctccg atctctctgc cggtatctcc
    19561 ggtgaagtgg tccacgttga cggcggtttc agcatcgctg caatgaacga actcgaattg
    19621 aaataatcat tctgttggta aatattgggc ggcgttctgc cgcccgttat ctctgttata
    19681 cctttctgat atttgttatc gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat
    19741 aacgatttcc tttacgacca aggagcgccc atggaacaac gccacatcac cggcaaaagc
    19801 cactggtatc atgaaacgca atccagtact gcggagtatg acgttctgcc tctggtcccg
    19861 gaagccgcaa aggtcagcga tccctttctg ctcgacgtga tccttgatga agaaacgcta
    19921 gctcccttcc tttcatggct ggtccctgcg cgcgttcttg cagtggaatt gttccctgac
    19981 cagcttaccg tgacccgttc acagaccttc accgcttatg aacgcttgtc tacggccctg
    20041 acggttgctc aggtttgcgg cgtccagcgg ttatgtaact actattcggc gcgacttacg
    20101 ccgctccccg ggcctgattc ctctagggaa agcaatcata ggttggcaca aatcacgcaa
    20161 tatgcccgcc aactggctag ctcgccttct attatcgaca accgatcgcg ccagcatctg
    20221 aatgacgtcg gtcttactgc cagggactgt gtaatcatta accaaatcat tggttttatt
    20281 ggctttcagg cgcggaccat tgcgacattt caggcttatc tcgggcaccc ggtacgctgg
    20341 ttacccggtc tggaaataca aaactacgcc gacgcgtcac tgtttgctga tgaatcaata
    20401 cgctggcgaa gcagctatga ggtggaaaaa ctacctgaag agtacacaaa aagttcaaca
    20461 gcagaacttt gccaactggc cgaaacactc tctctccacc ctatttcact ttcccttctc
    20521 gaaaggttgt taaacagtac acgggttaat acacagccgg ataataagct tgcggcgttg
    20581 ttatgcgcgc ggataaatgg cagtcctgct tgttttgccg cctgtatgga ttcatcaaat
    20641 gaatataaaa aaatcagccc ccttctgcgc aagggcgaaa atgaaattaa ccaatgggct
    20701 gaccgtcatt ctgttgagca cgctaccgtt caggcgatac aatggctgac ccgagcaccc
    20761 gatcgcttta gcaccgccca gttcagccct ttactcgaac acgaacaatc atcaacgcag
    20821 attattaatc tgctggtatg gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg
    20881 ttgggtgaga catattaacc ttgccgcgtc agacagattc gcgtaaaact gtcagccgct
    20941 ctaatggcca ccaaaataga caattatgtt tcaggacaac ccgctgctag cgcagcttaa
    21001 acagcaactg cattcccaga cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg
    21061 ctttggcttc ctggaagtcg acgcgcaaaa aagttatttc attccgccgc cgcagatgaa
    21121 aaaagtcatg catggcgacc gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc
    21181 cgcagagcca gaagaactgg ttgaaccgtt cctgactcgt ttcgtgggta aagttcaggg
    21241 caaaaatgac cgtctggcca tcgttcctga tcatccactc ttaaaagacg ccattccttg
    21301 ccgcgcagcc cgtggcctga accacgagtt taaagaaggc gactgggcgg ttgccgaaat
    21361 gcgccgtcat ccgctgaaag gcgatcgttc tttctatgct gaattgacac aatacatcac
    21421 ttttggtgac gaccactttg taccgtggtg ggttacactt gcgcgccata atctggaaaa
    21481 agaagcacca gacggcgtcg ctaccgaaat gctcgatgaa ggtctggttc gtgaagatct
    21541 gaccgcgctg gattttgtca ccatcgacag tgccagcaca gaagatatgg atgacgccct
    21601 tttcgctaag gcgttgccgg atgacaaact tcagctgatt gtggcgattg ccgatccaac
    21661 cgcgtggatt gctgaaggca gcaagctgga caaagccgcg aaaattcgcg cattcaccaa
    21721 ctatctgcct ggcttcaaca tccctatgct gcctcgcgag ctttctgacg atctctgctc
    21781 actgcgcgcc aatgaagtcc gcccggtact ggcatgccgc atgacgctct ccgctgatgg
    21841 cgccattgaa gataatatcg aattctttgc cgccaccatc gaatccaaag caaagctggt
    21901 gtatgaccag gtttctgact ggctggaaaa taccggtgac tggcagcctg aaagtgaagc
    21961 aattgccgaa caagtccgtt tgctagcgca aatttgccaa cgccgcggcg agtggcgtca
    22021 taaccacgca ctggtgttta aagatcgccc ggattaccgc tttattctcg gtgaaaaagg
    22081 tgaagtgctg gatatcgtcg ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc
    22141 gatgattgcc gctaacattt gtgcggcccg cgtactgcgc gataagctcg gttttggcat
    22201 ctataacgtg catatgggct ttgatccggc gaatgccgac gcgctggcag cgttgctgaa
    22261 aacgcacggt ctgcatgtcg atgccgaaga agtgctcacg ctggacggtt tctgcaaact
    22321 gcgtcgtgaa ctggacgcgc aaccaactgg tttcctcgac agccgcattc gtcgcttcca
    22381 gtcatttgct gaaattagca ctgaacccgg tcctcacttt ggcctcggtc tggaagcata
    22441 cgccacctgg acttcgccga tccgtaaata tggcgacatg atcaaccacc gtctgctgaa
    22501 agcggttatc aaaggcgaaa ctgcgacgcg cccacaggat gaaatcactg tccaaatggc
    22561 cgagcgtcgc cgtctcaacc ggatggcaga acgtgatgtt ggtgactggt tatacgcacg
    22621 cttcctgaaa gacaaagccg ggaccgacac ccgtttcgca gcggaaattg tcgatatcag
    22681 ccgtggcggc atgcgtgtgc gtttggttga taacggcgct atcgccttta ttccggcacc
    22741 tttcttacac gctatgcgcg atgaactggt
//
LOCUS       DAJVTY010000053        22760 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVTY010000053 DAJVTY010000000
VERSION     DAJVTY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22760
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.053"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..445)
                     /locus_tag="OQQ66_001855"
     CDS             complement(<1..445)
                     /locus_tag="OQQ66_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1904811.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGV"
     gene            complement(475..1191)
                     /locus_tag="OQQ66_001856"
                     /pseudo
     CDS             complement(475..1191)
                     /locus_tag="OQQ66_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1487..2263
                     /gene="exuR"
                     /locus_tag="OQQ66_001857"
     CDS             1487..2263
                     /gene="exuR"
                     /locus_tag="OQQ66_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1904812.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2608..3270
                     /locus_tag="OQQ66_001858"
     CDS             2608..3270
                     /locus_tag="OQQ66_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1904813.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3274..3657
                     /gene="mzrA"
                     /locus_tag="OQQ66_001859"
     CDS             3274..3657
                     /gene="mzrA"
                     /locus_tag="OQQ66_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1904814.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3804..4172
                     /locus_tag="OQQ66_001860"
     CDS             3804..4172
                     /locus_tag="OQQ66_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS1904815.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4210..4515
                     /locus_tag="OQQ66_001861"
     CDS             4210..4515
                     /locus_tag="OQQ66_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1904816.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4518..4922
                     /locus_tag="OQQ66_001862"
     CDS             4518..4922
                     /locus_tag="OQQ66_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1904817.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4912..5211
                     /locus_tag="OQQ66_001863"
     CDS             4912..5211
                     /locus_tag="OQQ66_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1904818.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5397..5789
                     /locus_tag="OQQ66_001864"
     CDS             5397..5789
                     /locus_tag="OQQ66_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1904819.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5859..6762
                     /locus_tag="OQQ66_001865"
                     /pseudo
     CDS             5859..6762
                     /locus_tag="OQQ66_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7055..7420
                     /locus_tag="OQQ66_001866"
     CDS             7055..7420
                     /locus_tag="OQQ66_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1904820.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7662..8018
                     /locus_tag="OQQ66_001867"
     CDS             7662..8018
                     /locus_tag="OQQ66_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1904821.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8069..8965)
                     /gene="yhaJ"
                     /locus_tag="OQQ66_001868"
     CDS             complement(8069..8965)
                     /gene="yhaJ"
                     /locus_tag="OQQ66_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1904822.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            9070..9771
                     /locus_tag="OQQ66_001869"
     CDS             9070..9771
                     /locus_tag="OQQ66_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1904823.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9794..9958
                     /locus_tag="OQQ66_001870"
     CDS             9794..9958
                     /locus_tag="OQQ66_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904824.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10092..11402)
                     /locus_tag="OQQ66_001871"
     CDS             complement(10092..11402)
                     /locus_tag="OQQ66_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1904825.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11430..12762)
                     /locus_tag="OQQ66_001872"
                     /pseudo
     CDS             complement(11430..12762)
                     /locus_tag="OQQ66_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            complement(13036..14400)
                     /gene="tdcG"
                     /locus_tag="OQQ66_001873"
     CDS             complement(13036..14400)
                     /gene="tdcG"
                     /locus_tag="OQQ66_001873"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1904826.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(14472..14861)
                     /locus_tag="OQQ66_001874"
     CDS             complement(14472..14861)
                     /locus_tag="OQQ66_001874"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1904827.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14875..17169)
                     /gene="tdcE"
                     /locus_tag="OQQ66_001875"
     CDS             complement(14875..17169)
                     /gene="tdcE"
                     /locus_tag="OQQ66_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1904828.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(17203..18411)
                     /gene="tdcD"
                     /locus_tag="OQQ66_001876"
     CDS             complement(17203..18411)
                     /gene="tdcD"
                     /locus_tag="OQQ66_001876"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1904829.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(18437..19768)
                     /gene="tdcC"
                     /locus_tag="OQQ66_001877"
     CDS             complement(18437..19768)
                     /gene="tdcC"
                     /locus_tag="OQQ66_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1904830.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19790..20779)
                     /gene="tdcB"
                     /locus_tag="OQQ66_001878"
     CDS             complement(19790..20779)
                     /gene="tdcB"
                     /locus_tag="OQQ66_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1904831.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20878..21816)
                     /gene="tdcA"
                     /locus_tag="OQQ66_001879"
     CDS             complement(20878..21816)
                     /gene="tdcA"
                     /locus_tag="OQQ66_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1904832.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            22005..22349
                     /gene="tdcR"
                     /locus_tag="OQQ66_001880"
     CDS             22005..22349
                     /gene="tdcR"
                     /locus_tag="OQQ66_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1904833.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5965 a         5758 c         5307 g         5730 t
ORIGIN      
        1 gaacaccttt ggtggtttga gcaaacatca ggtttttagc cagagaaccg ttagttaacg
       61 catcggaagc aaagattttg gttgcggtaa aagtggttgc cgcatcagct ttaatttctt
      121 caccgcccca ggttactgtc agcggaacaa tcttgctagc atccaggctt tgaaccagtt
      181 gcgccgggga aactaactgc atttttacat ctttggtaac gtcgttagac cagattttgg
      241 tcatcaactg gtaagattgc agaccctgcc ccggcagata ttgcatttct actgctttcg
      301 gcaacgcggt gttatcggtc tgtgtcatat ccagagcggc atcaacgttg gccgttacag
      361 taatatcctt ttgtactgcc aatgcggagc ctgcaacaga aaacatcgct gcgaccagaa
      421 gagattttgc aaataccttt ttcattacta ttccttcgtt tattacaatg ataattaatg
      481 gaccgccagt tcaaactcgc cattatcttt ggtgatccag ttattatatt tgacaacgat
      541 tttgtcgtag ccagatatgc tttcaagatt tctttcctga ccagggaaga tgtttttatt
      601 tacggtctgt atcttacaat tttctttgct attattttta caataagcga tcgttaaaat
      661 ctttaagcgt tgattccctg cattaactaa tttttgggca gcatttactt ctaacttagc
      721 aataggttgc tgaggtggaa cactaacaac aatcccccaa attaagctaa cagttaactg
      781 catagagaaa tcttttttat ctgtggcaac gtcatcaata ctgggaaccg cttcaaattt
      841 aacgcgttaa tttttttctt tttgctctgg ttccattgca acaaagcgca cagttttgct
      901 actaccggct ggtaaagcga atttaggtgg cataacaacc agatgatttg catcaccgga
      961 tttaatctca acttcatttt cctgaggcgt cgatggatta tcgatacgga atactgtcgc
     1021 cttgatgtac tgaacttcat tacttttaga tattacgcga acgttgcctt catcctgact
     1081 attaatggaa acagccatag gataaacagt catatttgac tgtgccaaaa aaggagcgaa
     1141 cacgaataaa agcggcgcaa gccgtgataa gagattaatt ttcgccacca tataaatttg
     1201 atcttacaat attgtttcca aaaagttggt gtattagaga tgtagattta ttgtaagtca
     1261 atataaaaaa gcatttttag ttaatattaa atttaaaggc tgggaattaa cttgctgaaa
     1321 tttagtagct ttcacaatga cgatacttat tttttaggaa tttataaata ttcacaatat
     1381 taattcaata cattaattca atcaccaaat ttatatatga ccaatggcat acccatgaat
     1441 aaagtggtat gataacttcg tccgcattcc actccgggag acgcatatgg aaatcactga
     1501 accacgccgt ttgtatcaac aacttgccgc tgacctgaaa gagcgcattg aacagggcgt
     1561 ctatctggtg ggtgataaac tgcctgccga acgctttatt gccgatgaaa agaacgtcag
     1621 ccgaacggta gttcgtgaag ccatcatcat gctggaagtt gaaggctatg tggaggtgcg
     1681 taaaggttca ggtattcatg tggtttccaa ccagccgcgc catcagcagg cggctgacaa
     1741 taatatggaa ttcgccaatt acggtccgtt tgagttgctt caggctcgcc agctcatcga
     1801 aagtaatatt gccgagttcg cggcaactca ggtaacgaaa caagacatca tgaaactgat
     1861 ggccattcag gaacaggcgc gcggcgaaca atgcttccgt gattccgagt gggatttaca
     1921 gttccacatt caggtagccc tggcgacgca gaactccgcc ctggcggcca tcgttgaaaa
     1981 aatgtggacc cagcgtagcc ataacccgta ctggaaaaaa ctacacgaac acattgattc
     2041 ccgtaccgtt gataactggt gtgatgacca cgatcaaatt ctcaaggcgt taattcgcaa
     2101 agatccccat gctgctaagc tggcaatgtg gcagcatctg gaaaacacca aaatcatgtt
     2161 atttaacgaa accagcgacg atttcgagtt caatgccgac cgctatctat tcgccgaaaa
     2221 cccggtagtg catctcgata cggccaccag tggcagtaaa tgaaactcct gtccgacagg
     2281 cgctgcgacg cgcctgttcg cttcgtcggt aagcgaaaag tataaagtgt cagcctgtgt
     2341 aaatcctctc gccaccctcc cctgcattca gcaaaatcag actccacgga catgtaattt
     2401 tgataacgaa caacgttgac ctttgttaca attagattca atttgaattt atgtttttga
     2461 atgctttctt atctcacgat ttaacaggga atagttcagg ctgtgttgat gtatcaaacc
     2521 cgcagaacat accaaaacag caataacatt gcggtagtgc atcttttaaa accagcgtgg
     2581 cgttaaccga ttcaccagga ataatgaatg gaacttttga cccaattgct gcaagccctg
     2641 tgggcgcagg attttgaaac cctggccaat ccatcgatga ttggcatgtt gtattttgtc
     2701 ttgtttgtaa ttttgttcct tgaaaacggc ttgcttccag cggccttttt accgggcgac
     2761 agtttactgg tattggtcgg cgtgttgatt gcgaaaggcg cgatgggtta cccgcaaacg
     2821 attctgctgc tgaccgttgc cgccagcctc ggctgctggg tcagctatat tcaggggcga
     2881 tggctgggca atacccgcac cgtacaaaac tggctatctc atttacccgc gcattatcat
     2941 caacgcgcac accatctttt tcataaacac ggtttatcgg cgctgttaat tggtcgcttt
     3001 attgcgtttg tcagaacact gctgccgacg attgccgggt tatcagggct gaataacgcg
     3061 cgctttcagt ttttcaactg gatgagcggt ctgctgtggg tattgatcct gacaactctg
     3121 ggttacatgc tcggcaaaac gccggtattt ttaaagtacg aggaccagct gatgtcatgc
     3181 ctgatgctgc tcccggtggt gctgctggtg tttggcctgg caggttctct ggtcgtgtta
     3241 tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa tacctcgcat gtcgcttcgc
     3301 cagctagcct ggtccggcac tgttttactt ctggtcggca cgctgttgct ggcctggtcc
     3361 gcggttcgcc agcaagagtc tacgctggcg attcgtgccg ttcatcaagg cacaacgatg
     3421 ccagacggtt tttcgatctg gcatcacctt gacgctcatg gcattccttt caaaagtatc
     3481 acccccaaaa acgacactct gttaattaca tttgactcca gcgaccagag cgccgccgca
     3541 aaagcggtcc tcgacagaac attgccccat ggctacatca ttgcgcagca ggacaataac
     3601 agtcaggcta tgcagtggct gacccggtta cgggataact ctcatcgctt cggataactt
     3661 cctgttatcc gaaacatatc actcactttg gtgatttcac cgtaactgtc tatgattaat
     3721 gaggcggtgg ttaacactac cgcttcgttg tctggacaat cgttcctttg taacaggtcc
     3781 gaatcacaat ggaaggttca agaatgaaat accgcatcgc tttagctgtt tctctctttg
     3841 ctcttagtgc cggtagttat gccactaccc tgtgtcagga aaaggagcaa aatatcctta
     3901 aggagatcag ctatgccgaa aaacaccaaa accagaatcg tattgacggt ctgaataaag
     3961 ccctgagtga agtccgggcc aactgttcag atagccagct gcgtgccgat catcacaaga
     4021 aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca agatttagcc gaggcgaagc
     4081 aaaaaggcga tgccgataag attgccaaac gcgaacggaa actggcagaa gcgcaggaag
     4141 agctgaaaaa gctggaagcg cgcgactact aactcacaat agtcactact tactcacctg
     4201 gagaaaacta tgtcgaaaga acacactacg gaacatctgc gtgctgagtt gaaatccctt
     4261 tccgatacgc tggaagaggt gcttagctca tctggcgaga agtcgaaaga agagttgagt
     4321 aagattcgta gcaaagcgga gcaggcactg aaacagagcc gttatcgcct gggtgaaacc
     4381 ggtgatgcca tcgccaaaca aacccgtgtc gcggcggcgc gtgctgatga gtatgtgcgt
     4441 gaaaatccgt ggaccggcgt gggaattggc gctgcaatcg gtgtagtgct cggcgttctg
     4501 ctgtcgcgtc gttaattatg gcggacactc atcacgcgca agggcccggt aaaagcgttc
     4561 tgggcatcgg gcagcgaatt gtttccatca tggttgaaat ggtggaaaca cgtctgcggc
     4621 tggcggtggt ggagctggaa gaggaaaaag ctaatctctt tcaactttta ctgatgctgg
     4681 gcctgacgat gcttttcgct gcatttggtc ttatgagcct gatggtgcta attatttggg
     4741 cggttgatcc gcaatatcgc ctgaatgcga tgattgccac caccgtggtg ttgctgctac
     4801 tggcactgat tggcggtatc tggacgttac gtaaatcgcg taaatccacc ttgctgcgcc
     4861 atacacgcca tgagctggca aacgatcggc aactgctcga ggaggagtcc cgtgagcagt
     4921 aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc agatccagca acaacggctg
     4981 gatctttccg ccagtcgtcg tgaatggctg gaggcaacag gcgcttacga tcgtcgctgg
     5041 aatatgctgc taagtctgcg ctcctgggcg ctggttggca gtagcgtgat ggcgatctgg
     5101 acgattcgcc atcctaatat gctggtccgc tgggccagac gcggttttgg cgtatggagc
     5161 gcctggcgtc tggttaaaac gaccctcaag cagcaacagc ttcgcggtta acttccctct
     5221 ggccggagcc attccggccc tatccctcaa attttttgaa gatttttgac agttttcctt
     5281 gctaacaatc atcattcacc acgtttatga ttctctccat cgacagcaac gacgctaata
     5341 ccgcgccctt gcacaaaaaa acaatcagca gcctgagtgg cgcagtggag agtatgatga
     5401 aaaaattaga agatgttggt gtactggtag cgcgcatttt aatgccgatt ctgtttatta
     5461 ccgcaggctg gggaaaaatc accgcttact ctggtaccca acaatatatg gaagcaatgg
     5521 gtgtcccggg ttttatgctg ccgctggtga ttctgctcga gttcggtggt ggtctggcaa
     5581 tcctgttcgg tttcctgact cgcaccacag ccctgtttac tgcgggcttt acgctgctga
     5641 cggcattttt atttcacagc aagtttgctg aaggcgtcaa ctcgctgatg ttcatgaaaa
     5701 acctgacaat ttctggcgga ttcctgctgc tggcaattac cggtccgggc gcgtatagca
     5761 tcgaccgcct gctgaataaa aagtggtaag cacgcgctat acttaacgat aaaaaacgag
     5821 gaggaagctc ctcgtttttg ctattggagg agagaaaaat ggggcaactg attgacggcg
     5881 tctggcatga cacctggccc cactggcaca ggcggtttta tcgcagagaa agatcgttat
     5941 catctctatg tttcactcgc ctgcccgtgg gcgcaccgca cgctgatcat gcgcaaactc
     6001 aaaggactgg aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct ggaaaacggc
     6061 tggacctttg atgacagttt tccgggagca accggagata cgctctatca acatgaattt
     6121 ctgtatcagc tttatctcca cgccgatcca cactacagcg gacgagttac tgttcccgtg
     6181 ctgtgggaca aaaagaacca caccattgtc agcaacgaat cagcagaaat catccgcatg
     6241 ttcaataccg cgtttgatgc gctgggcgca aaagcgggtg attactaccc accagccctg
     6301 caaacgaaaa ttgacgaact taacggctgg atttatgaca ccgttaacaa cggcgtgtat
     6361 aaagctggtt ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa agtgtttgaa
     6421 tcgctggcgc gactggagca gattttaggc cagcatcgtt acctgaccgg caaccagcta
     6481 accgaagccg atattcgcct gtggactacg ctggtgcgtt ttgatccagt gtatgtgacc
     6541 cacttcaagt gtgataagca ccgcatcagc gattacctga atctgtatgg cttcctgcgc
     6601 gatatctacc agatgccggg gatcgccgaa actgtcaatt tcgatcacat ccgtaatcat
     6661 tacttccgca gccataagac catcaaccct acggggatta tttcaattgg tccgtggcag
     6721 gatctcgatg aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc accttacgag
     6781 gtgcctctga taattagcta agtgcatgtt gttcatgtcg gatgcggcgt gaacgcctta
     6841 tcaaacctac aaaacgttta tattcaatat gttaaaatga ccgcgtaggc cagataagcg
     6901 tagcgcatct ggcgattttg cgcttgtcat cagtcttagc gcaccttcac ggcactttat
     6961 taattccttg aacgaatatt tactgccatt tcattcacgt ctattcttaa tttgctgctt
     7021 taaaaacaag tgattgagca aaattgaggc aaaaatggac tggtatctga aagtactaaa
     7081 aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt
     7141 caacatcatc tttacgttcg tgctggggct gctggataaa atgttaggct ggcaacgcgc
     7201 cggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac cgtggtgggc
     7261 ggttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac tgttgttctt
     7321 aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg gtacgccagg
     7381 cgaaaaccgc tttggaccag atccgaaact ggaaccttaa aacaaatctg ctcgccggat
     7441 gtctgactca tccggctttt atcatcatgt tgatgaatga ataatttact aatgtttatt
     7501 taaaatattt caatgtcgtt atataaatta cctataaaaa acaaccatgt atttatctta
     7561 aatatcaaaa cctttataac atccgaaaaa gaaagatacg aatagcattt acacaatacc
     7621 cggaatagac tttcgtcact tttaattaaa gggatgtttt tatgcagtgg tatttagccg
     7681 tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg taaagagtac tggatgttta
     7741 ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt ttaggtttag
     7801 agtttccatt cctttctctc atttacctgg cagcaacaat cattccggtc attgctctgt
     7861 gtgttcgtcg tctgcacgat actgaccgtt caggtgtgtg ggcgcttctc tatctggtac
     7921 cgatcattgg ctggttagtt ctgtttgtat ttgcctgttt agaaggcaat tctggcagca
     7981 atcgttacgg aaacgatcct aagtttggtt caaattaatc ttagaattgg ggcgatattt
     8041 cgcccctttt tattaacaat aataaatatt attttccgct aaaaagtttg ggaatttccc
     8101 gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata ataatatcga
     8161 tctcgctggt cgattccggg ctgacgacac gcaaccgccc ttccgcaata tctttttcga
     8221 ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga cgtttatctt
     8281 caatcgtgct caccgttaag cgcggctgtt tgtccagcag ctgtacggtc aacaccgggc
     8341 gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc acttcagata
     8401 acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg ttcattaagg
     8461 tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc ggcgcgataa
     8521 cgatatccgc ccgcccctgc tccagtcgtt cccacgcccc cgccagcact tcggtgatga
     8581 ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa aaggcaggtg
     8641 tcggtaccag cgcctcggtc acaatggtga gatgcgtttc ccaaccgcgc gcgagggctt
     8701 ccgcatcggt agtcagttta tctgctgctt ccagcaaaac gcgccctcgc tccagcaaca
     8761 tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc accacatcca
     8821 gctcttcttc cagtttttgc atggtgtagc taagtgcgga aggcacgcgt cccagctcat
     8881 ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt agtgcttcca
     8941 gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa cataccgggc
     9001 agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag taaagagagg
     9061 tcaagaatta tgattactac ccgaactgcc aggcagtgtg gacaagcaga ctacggatgg
     9121 ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt gttaggctat
     9181 gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt tcagccgcga
     9241 acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga gtatcgcgac
     9301 agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc tacccagccg
     9361 ggtgtcagct acagcgaaca caatctcagc aaagacaaac cgttaacacg aatgcagctt
     9421 tggctggacg cctgcccgca gcgagagaat ccgctgattc aaaaactggc gcttaatatg
     9481 ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg gaagcctgca attacgccag
     9541 caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt ccagttgcat
     9601 gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac gcatcatgaa
     9661 gagaaagcgg cgctgacctg cggtgatggg gcgtttattc gtgacgaggc taacattacg
     9721 ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta gttgttttta
     9781 cagggagatg atgatgagta aaaaatcggc caaaaagcgc cagccagtga agcccgtggt
     9841 ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt tgagcgagct
     9901 ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta ccgcgtaaag
     9961 acaggttgcc ggatgcggcg aaaacgcctt atccggccta caaaatccaa caacattaac
    10021 gaattaaaca acccgtaggc cggataaggt gcgccagcat cgcatccggc acaatactca
    10081 aaacctggcg tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc
    10141 tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc
    10201 acgatccctt cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac
    10261 gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgacgcacc atcgcaaatc
    10321 atgccgctga catcgccgat catactgctg atcgccatcg agatagtttc ataacgccca
    10381 tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc ggcacaaagt
    10441 gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgaga aagcatcagc
    10501 gcacgcgcca gtcgttcatc atccgctccg aagtgttctg ctacaaccac cacaggcatt
    10561 gttgcggtga tcccctggtt acccgagccg gagttactca tagccggaag cgtagcgccg
    10621 cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc
    10681 gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc caatatgcag cccccactta
    10741 ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag aataaagcgg
    10801 atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt cgttctggaa
    10861 agcaccgtca gcggagactc ttgctcgccc tctgccacac acgcctgctg ggtaaacacc
    10921 acactattgt gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat ggtgacacac
    10981 gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatt gcaaggttcc
    11041 tggatcttaa cggagacttt ccccgccgcc agcagtgctt tggcatcggc aattgcctgc
    11101 gcagttgcgt ctttcagcac ttccagcccg gcgttggcat ttccacctaa cgcccccagc
    11161 gccgccgcaa tcggcagccc caccattccc gtgccgggaa cggtgacgcc cagaccgttc
    11221 ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttctgcc
    11281 gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag cgccggtttt
    11341 acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac
    11401 atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa
    11461 agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt gtgcaatgcc
    11521 ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta
    11581 cagatagagg tgaagctcaa caccggtgcg ttcagtacga tggcgctcca ggccagcaaa
    11641 atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt taatcttctc ggcaggcatc
    11701 ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg
    11761 ccaaagaatg cagtcatgac agcaaagata ttgagaatga cgctgaccac tttcacccac
    11821 gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca gcgcggaaat attctgctcg
    11881 taggctttta ccgcttcgtc atgtcccatc gccagcgtga acgacacggc gtagaaaaag
    11941 acggtgacaa ataaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc
    12001 acttcaattg atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata
    12061 aacagaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcac cagcagcccc
    12121 agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc
    12181 gcagcgacta ccagcagctt ggtcagcacc atgccggtcg aaattttgaa taacaatttc
    12241 tcgccgcgtg aagagatcgc caccagaatg caaatcagta ccagaccata aaagggactg
    12301 tctgacagca ccccttccgt cacgccgaag gtatgcagat aggaagcact atcgttggtg
    12361 atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag cgcacctaac
    12421 aggatgcccc agtttttacc taaataaccg ctaatgacac tcgggtaatc tttacattct
    12481 gtgtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
    12541 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
    12601 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
    12661 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
    12721 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt
    12781 ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg
    12841 caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc
    12901 aagactttga aataacggag atggataaga attttctact aaattaatcg caagagaata
    12961 ttcattctct atttgcggca gatcacaaaa aaaggtgcac atttgtgcac ccaaggatga
    13021 aagctgacag caatgtcagc cgcagaccac tttaatggcc agtcctccgc gtgatgtttc
    13081 gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag
    13141 tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta ctgctttcac
    13201 ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca
    13261 ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact
    13321 gccgcccagt agttcagtta accctgccgc cgccattgaa caggccacgc caatctcccc
    13381 ctgacagccg acctccgcgc cagagatgga ggcgttcatt ttatacagcg cgccaatagc
    13441 ccccgctgcc agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc
    13501 gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac
    13561 gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatgttga tccagtcgat
    13621 gacattcatc ggatcgttag agatgttatc gctagaaacc agctgacgac gcagcgctac
    13681 ggcacgacgc ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc
    13741 aataccgtca tgcatcactt gccagatacg ggcaaaccct gcgtcaattt ccgctttgct
    13801 gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt tgtaatcaca
    13861 cattttcagc agttcacctg ctgagtggaa atcgtaaggt acggacgttt cgacatcgtg
    13921 cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt
    13981 acttaatagc gcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg
    14041 caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc
    14101 agatgccact ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc
    14161 aatgacaaca tcctgcggac tgtttcctgc cagtcccatg ataatggcga catccgtggc
    14221 atggcctttg cccgtcagtg acaacgcccc atacagatcg accacaatat ggctcgtcgc
    14281 ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc
    14341 cacggtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat
    14401 atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg
    14461 ctcacgaggc tttacgcact acgtactgcg atagcttcaa tttccagctt cacatctttc
    14521 ggcaaacgcg caacctgcac acagctccgg gtcgggtagg tcgcctgatg ctcatcgaag
    14581 aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat gaacacggtc
    14641 atcttgatga tatcgcccac gctcagcccg gcagcaacca cgatcgcttt gacgttttcg
    14701 aggcttaaac gcgcctgatc ttgcacatca gccgggatct caccggtctg cgggcaaacc
    14761 ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg
    14821 attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag
    14881 cgcctgggta aaagtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa
    14941 gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat
    15001 ggcatccagc agcatttccc gattcattat gttgacgttg aggtgttgac cgccttcgac
    15061 atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg
    15121 atcttctttg cccagcgccg caggaacgat tgagaaggtg tacgagatcc catctttggc
    15181 gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcacctt tgcggtcacg
    15241 accatgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcgacgac cgtccggcgt
    15301 attaccggtt ttctggccgt acaccacgtt agaagtgata gtcagaatcg actgggtagg
    15361 gacggcgttg cgataggttg gcagcgcttt aattttcttc ataaagcgtt caaccaggtc
    15421 gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac tgcggatatt caccgtcgat
    15481 ttcaaagtcc accgccaggc cgttttcgtc acggattggt ttcacgcggg catatttgat
    15541 ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg ccgcatgcca tagtgcgata
    15601 gacatcacga tcgtgcagcg ccatcagcga agcttcgtag ctgtacttgt cgtgcatgta
    15661 gtgaatgata ttcagcgcgc tgatgtactg caccgccagc cagtccatga agtgatcgag
    15721 gctgtccatc actttgtcgt aatccagcac gtcgtccatc agcggtgctg ttttcggccc
    15781 gacctgaatc ttcagcttct cgtccacccc gccgttaatt gcgtagagca gcgttttcgc
    15841 caggttagcg cgtgcaccaa agaactgcat ttgcttacca atcaccattg ggctgacgca
    15901 gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc atcagatcgt cattttcata
    15961 ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat tttttgaagg caatcggtaa
    16021 ttcttccgac caaagaatgg tcaggttagg ttccggtgcc ggccccatag tgtgcagggt
    16081 gtgcaaatag cggaaggagt ttttggtcac cagcgtacga ccgtccagcc ccatcccgcc
    16141 gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc gaatcaaatt ccggtgtacg
    16201 caggaagcgt accatacgga tcttcatgat gaagtgatcg atcagttcct gtgcctgctg
    16261 ctcattgagt acgccagctt caaagtcgcg ctcaatgtag atatcgagga acgatgccgt
    16321 gcggcccagc gacatcgcgc cgccattttg cgatttcact gccgccagat aagcgaagta
    16381 gagccactgc accgcttctt gcgcattctg cgccgggcga gagatatcaa agccatattt
    16441 cgccgccatt tcctgaatct gcaacagcgc atgacgatgc tctgccagct cctcacgcag
    16501 acggatggtg gcttccagat cctcgccttt ttccagacga gactggagat cggcaaattg
    16561 cagttcgcgt tcacgtacca gataactgat gccatacagc gctacgcggc gatagtcacc
    16621 gataatgcgc ccacggccat agccatctgg taaaccggtc agcacgccag atttacggca
    16681 gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc
    16741 ggtaaacaga tattcaaatt cactgtccat ttcacggcca taggcgtgga atgaactttt
    16801 aatcatatta atgccaccga acgggtgtag cgcacgtttc aacggcgcat ccgtttgcag
    16861 gccaacaatt ttttccagcg gctggttaat atatcccgca tcatgagcgg taattgtggt
    16921 ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg ttttcgatac ggataccttc
    16981 cattactttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagga aagattcatc
    17041 gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca
    17101 gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc
    17161 taccttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcggg
    17221 cgcgttaact ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttagtcgg
    17281 aataacagcg caaatgacac gcgcattttc actggaaaca attcgctcac cacaggagtt
    17341 agagcgatta ttcatttctg tatcaatctc tacgcctaat acagccaaat gttccatgac
    17401 cagacgacga attaagcttg aattctctcc tattccgccg gtgaatataa ttccatccag
    17461 gcgacgtaat gaagctgcgt gtccggcaat atgacgggca attcggtgaa caaaggtttt
    17521 aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc
    17581 cgaagaaaga ccggaaatac ctaataatcc cgactcttta ttcactacgc gttccaggtc
    17641 acccaagctc tggttggttt ggctggcgac ccaggacatc gcaccaaaat cgacatcgcc
    17701 actgcgggta cccatcatca agccttccag cggcgtcatt cccattgagg tgtcaacact
    17761 ctgaccgttg cgaactgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc
    17821 ggagtcatct tccgctagat tcagcaaaga atgtgcgcgc tgggaaacat agcggtgcga
    17881 cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc
    17941 gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgccacctg
    18001 agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg
    18061 caggggtgcc agtggagaaa cgcgacggat attatcaatg acctcatcgg taataatggc
    18121 ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc
    18181 atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg
    18241 gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt ctgagttaat
    18301 accgtcggca atacctgaca ttaatacttc acagtcgctg gcatcgagta cggaaaactt
    18361 aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct
    18421 catcctgagt tacggattaa aacagtttgt atacgatgtt caggatggtc agcagaccaa
    18481 tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac ggtgctttac
    18541 ggatggcata catcggcaac aggcacagca gggatgcgat aatcggtgcg cccatggctt
    18601 caataaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga
    18661 agatcatgct gatagtgttc agtttaccca gcgacacttt agttttgtcg cctttataac
    18721 cgaacttcag aatcagacca ttcaagcctt ccagcgtccc cagatagtga ccgaagaaag
    18781 atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg
    18841 ttgttttggt accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat
    18901 tctgcgcttt ggcttccgcc atgttggccg gagacagcgt aaacaggcag ctaaaggcaa
    18961 agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac
    19021 gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg
    19081 aagagacgat tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc agccagacag
    19141 tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa
    19201 ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg
    19261 gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag
    19321 ccatcagcag caacaggaac agcgccacaa agccgcgatt cagcggtgca aatccgagct
    19381 ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg
    19441 ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct
    19501 cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacac agacgcgcca
    19561 gcgcccggtg gcaataaaat gcgatggggt atgccaatac caacatcaga agaatcggga
    19621 tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac
    19681 caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt tttgtctggc
    19741 tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa
    19801 cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga
    19861 cggtttttct gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca
    19921 gagcacctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc
    19981 tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag
    20041 ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg
    20101 tggttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttct gactgtacgc
    20161 caataacacg aatggtcggg ttaatagatt taattgccac cgcaatacca gcaattaaac
    20221 caccaccacc aattggcaca atcacgttat cgacatcata gagatcttcc ataatttcca
    20281 gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg ataaaaatac
    20341 gaccttccat ttcgacaatt tcgctcactt tagcgatagt gtcgttgaag ttatcaccat
    20401 gcagaacgac ttctgcggag tagtcgcacg ttgccgctac tttggatttt ggcgcacctt
    20461 ttggcatcac cactttaccg tcgataccca gcatcgcgca ggagagcgaa accccttgcg
    20521 catggttgcc cgcagaacag gccaccacgc ctttgcgttt ttccgcatcg gtcagtgaac
    20581 ttaatttatt aaatgcgcca cgaattttaa atgaacccgt acgctgcata ttttcaaact
    20641 tcaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc atgcctgttt
    20701 tataaattcg cccagccagt cgttgtttcg cttcaataat atcatcaata gcaaccggca
    20761 gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa ttaaatatgt agataaccgt
    20821 aaccgacacc tgcaaaacag acaggtggat tatttatgtt taaataaaaa caaataacta
    20881 accaacttct attaatagcc ttcgtctaca cccattataa gatgaatact ctttggctaa
    20941 ttccaccaaa accgatgctg cttttttaat acgataattt ttcgaccata ccgcggcata
    21001 ttgtgccaca ggtaatgttt cttcaactgg aatagtaata aattgatttg aaccaaaagg
    21061 tgacgtcata tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa gattataaat
    21121 tgtcacgact gagtcggttt taacgatgtt ttcaatactg atgccatttc tttgtaacgt
    21181 agtaagaagc tcgctgtagt accccatatt ggtttgtggc aacacccact gttcgttctt
    21241 caacgactcc agcgtggtgg tgccggtgca tgttcgggac ttactggcta ccagcacaaa
    21301 ctcggactcg aacagcggct caacatgtaa atcctgaagc ttcatttctg cacttaacgt
    21361 accaatcgca aaatccagtc gaccgtcgcg gattgccggt aagaacgaag acagttgcgc
    21421 ttcatacata gaaacctgcg ctttcgggaa cacctctttg aacttgttga tcatccctga
    21481 cataaaagta aaaccaatca atgaaggaaa accaaatgag acttccacca ccgcctcaga
    21541 agacataccg cttatctcat taaccatatt tttcatttca cgggtaatgg attcggaacg
    21601 ggagagtaac aattgaccgg caggtgttaa tgttacaccg gtatttttcc gcaccactaa
    21661 ttccacacca aaataatctt caatatcgtt aatgatttta ctgacggccg gttgagttaa
    21721 ccctaattct tttgcagccg agccgataga gccacttcta atgacttcct gaaagactac
    21781 caggtgctgc gttttcggaa gaagaatagt gctcataacg acctacgtta attacctcat
    21841 tgacggcatg aagtgtatca aaatgaaatg aacaggatat gtgcgaccac tcacaaatta
    21901 actttcatta ctttccaggg tatcgttatt acaattaaat atttttaaat atcaacaagt
    21961 taaaacataa aaatcagcat aaaccctgat ttttatcaaa aaaaatgacg gggataacca
    22021 tattttatgg cgataacatc attcgttatg tggttaatac aaaaaagggg ctgagaccat
    22081 atttcaagca attaccggat aattaccagg caaaatttga gttaaattta atgagcaagt
    22141 tctcaaattt tattataaat aaaccatttt cagcgataaa tactgcggca cgtcacattt
    22201 tttcacgcta tttgttggag aacaaacatt tattttatca atattttaaa atttcgaata
    22261 caggtattga tcatctcgaa caattgatta acgtcaactt tttctcttct gacaggacgt
    22321 cattttgtga atgcaatcgt tttccataaa ttcttctccc ctcataggcg acgaatagca
    22381 ttttgtgttg aggatcacaa aacaaataat tgctggtcgc cgcgataagg tcagacgaag
    22441 tcaacaaggg gaattttcac tgtgctttac atcagcgtag gacacagaat gttgcttcgt
    22501 ttactgtaac gccgggtaaa tgagcgtttt ttgatagtgc gtaagtgtcg tgcgaggaaa
    22561 ataagcatct attattgttt gtcatgacga caaggcaggc atcaatgaaa ggcttcccaa
    22621 ttgcacatat ttttcaccct tcaatcccgc caatgcacgc agtggttaac aatcacaata
    22681 gaaatattga ttattggacg gtaaaaagaa agtttgcaga aattgtctcc accaatgacg
    22741 ttaaaaaaat ttacagtata
//
LOCUS       DAJVTY010000054        22596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVTY010000054 DAJVTY010000000
VERSION     DAJVTY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22596
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.054"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            41..970
                     /gene="aaeR"
                     /locus_tag="OQQ66_001881"
     CDS             41..970
                     /gene="aaeR"
                     /locus_tag="OQQ66_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1904834.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1098..2543)
                     /gene="tldD"
                     /locus_tag="OQQ66_001882"
     CDS             complement(1098..2543)
                     /gene="tldD"
                     /locus_tag="OQQ66_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1904835.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2699..6499)
                     /gene="yhdP"
                     /locus_tag="OQQ66_001883"
     CDS             complement(2699..6499)
                     /gene="yhdP"
                     /locus_tag="OQQ66_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1904836.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDATAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6567..8036)
                     /gene="rng"
                     /locus_tag="OQQ66_001884"
     CDS             complement(6567..8036)
                     /gene="rng"
                     /locus_tag="OQQ66_001884"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1904837.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8026..8619)
                     /gene="yhdE"
                     /locus_tag="OQQ66_001885"
     CDS             complement(8026..8619)
                     /gene="yhdE"
                     /locus_tag="OQQ66_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1904838.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8628..9116)
                     /gene="mreD"
                     /locus_tag="OQQ66_001886"
     CDS             complement(8628..9116)
                     /gene="mreD"
                     /locus_tag="OQQ66_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1904839.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9116..10201)
                     /gene="mreC"
                     /locus_tag="OQQ66_001887"
     CDS             complement(9116..10201)
                     /gene="mreC"
                     /locus_tag="OQQ66_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1904840.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10267..11310)
                     /gene="mreB"
                     /locus_tag="OQQ66_001888"
     CDS             complement(10267..11310)
                     /gene="mreB"
                     /locus_tag="OQQ66_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1904841.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11615..13555)
                     /gene="csrD"
                     /locus_tag="OQQ66_001889"
     CDS             complement(11615..13555)
                     /gene="csrD"
                     /locus_tag="OQQ66_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1904842.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13707..14681
                     /locus_tag="OQQ66_001890"
     CDS             13707..14681
                     /locus_tag="OQQ66_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1904843.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15659..16129
                     /gene="accB"
                     /locus_tag="OQQ66_001891"
                     /gene_synonym="fabE"
     CDS             15659..16129
                     /gene="accB"
                     /locus_tag="OQQ66_001891"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1904844.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16140..17489
                     /gene="accC"
                     /locus_tag="OQQ66_001892"
     CDS             16140..17489
                     /gene="accC"
                     /locus_tag="OQQ66_001892"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1904845.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17581..18628)
                     /locus_tag="OQQ66_001893"
                     /pseudo
     CDS             complement(17581..18628)
                     /locus_tag="OQQ66_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18625..19587)
                     /locus_tag="OQQ66_001894"
     CDS             complement(18625..19587)
                     /locus_tag="OQQ66_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1904846.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPQEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19609..20598)
                     /locus_tag="OQQ66_001895"
                     /pseudo
     CDS             complement(19609..20598)
                     /locus_tag="OQQ66_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20599..22098)
                     /locus_tag="OQQ66_001896"
     CDS             complement(20599..22098)
                     /locus_tag="OQQ66_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1904847.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22159..>22596)
                     /locus_tag="OQQ66_001897"
     CDS             complement(22159..>22596)
                     /locus_tag="OQQ66_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1904848.1"
                     /translation="AIISYPALQSVVARVDGFKKGLSDTPDIKIVAEQPSITRAEALT
                     TAQNIMQANANLNGLFGFGDDAALAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKT
                     FAAVIRQYPDQMGAKAIDAAVDHLNGKPVEKMIPVQPGVYTGK"
BASE COUNT         5597 a         6122 c         5405 g         5472 t
ORIGIN      
        1 atattatgtg atctaaatca cttttaagtc agagtgaata atggaacgac taaaacgcat
       61 gtcggtgttt gccaaagtag ttgaatttgg ctcttttacc gccgccgcca gacagctaca
      121 gatgagcgtt tcgtccatca gtcagacggt gtcaaaactg gaagatgagt tgcaggtaaa
      181 gctgttaaac cgtagcacac gcagcattgg cctgaccgaa gccggtagaa tttactacca
      241 gggctgccgt cgtatgcttc atgaagtgca ggatgttcat gagcaactgt atgccttcaa
      301 taacaccccc atcgggacgc tacgcattgg ctgttcttca actatggcac aaaatgttct
      361 cgccgggctg acagccaaaa tgctgaaaga atacccaggt ttgagcgtca atctggttac
      421 cggaattcca gcccccgacc tgattgccga cggtctggat gtggtgatcc gcgtcggcgc
      481 gttgcaggat tccagcctgt tttcccgccg tcttggcgcg atgccaatgg tggtgtgcgc
      541 cgcgaaaagc tatctcacac aatacggcat accggaaaaa cccgccgatt taagtagcca
      601 ttcatggctt gaatacagcg tgcggcccga caatgaattt gaactgattg caccggaagg
      661 gatctcgact cgcctgatcc cgcaagggag atttgtgact aatgatccga tgacgctggt
      721 gcgctggcta acggcgggtg ccgggatcgc ctacgtgccg ctgatgtggg tgatcaacga
      781 gatcaatcgt ggggagctgg agatcctgct gccgcgttat cagtcagatc cacgcccggt
      841 ttatgcgtta tataccgaaa aagataagct gccgctgaag gtacaggtcg tgatcaactc
      901 gctgacggat tattttgttg aggtcggtaa attgtttcag gagatgcacg ggcgcgggaa
      961 agagaagtaa ttcatattgt actgttacgt tctacaaacc tgtgccaacg ggttcccctc
     1021 accctaaccc cagaggagcg aggggaccgt tcgtgcacgt agaaagatta attatccttc
     1081 tgaaaataat gaaattatta cgcagtaccg ccaacagtca ggttatcaac tttcagcgtt
     1141 ggctggccca cgccaaccgg caaactttgc ccttctttac cgcagacacc cacgccgtta
     1201 tccagtttca ggtcgttgcc aaccatcgaa atctgctgca tggtttcgat accggaacca
     1261 atcaacgttg cgcctctcac cggcttcgtt actttaccgt tttcaatcag atatgcttct
     1321 gaagtggaga aaacgaattt gccggaggtg atatccacct gaccgccacc aaagttcggt
     1381 gcatagatac cgtactcaac ggattcaata atttcctgcg gggtcgattt acccggcagc
     1441 atataggtgt tggtcatacg cggcatgggc agatgggcgt aggattcacg gcgaccgttg
     1501 ccagtcggcg tcatccccat caaacgcgcg ttgagtttat cctgcatgta gcctttcaga
     1561 atgccgttct caatcagcac gttgtactgg cctggcgtac cttcgtcatc aatcgccacc
     1621 gaaccacggc gatcgaccat cgtgccgtca tcaaccacgg tacacagttc tgaagccacc
     1681 agctccccga cctgcccact aaatactgaa gtgccgcggc ggttgaagtc gccttccaga
     1741 ccgtgaccta ccgcttcatg caacagcacg cccggccaac ctgcgccaag tactaccggc
     1801 atggtgcccg ctggtgcagc aacggcagaa agattgacca gcgccatacg cactgcttct
     1861 tttgcccatg catccgcacg gacttcgccg tcaagatcgg caaggaagaa ctcataacca
     1921 aaacgaccgc caccgccact ggcaccgcgt tcgcgtttgc catcttcttc gaccagaacg
     1981 ctcacggaaa gacgcaccag cggacggaca tccgccgcta gcgtgccgtc ggtggccgca
     2041 accagaatta attcatagac accactgagg ctggcagtca cttcctgtac gcgcttgtcc
     2101 gcttcgcgag caaccttatc gacgcgacgc aggatatcca gcttctcttc acggctcatg
     2161 ctttgcagcg gatctaccga ggtatacaac gggctatgcc ctaccgcgcc cagcgtctgt
     2221 actttgccat caccactatc acggacgatg gtgcgcgccg cttgcgcact ctgttccagc
     2281 gccagcaggc tgatttggtc agcgtaagca aatccggttt tttcaccgct gattgcacgc
     2341 acgccaacgc cctgatcgat gttgtaagaa ccatctttaa taatgcggtc ttctaaaacc
     2401 caggattcgt gatagctcga ctgaaaatag agatcgccat aatcaaggcg acgttcggcc
     2461 agttgaccga ggatcgcgaa caagtcctga tgtttcaggc cgttcgccgc tagcaattgt
     2521 tcacttacta ggttaagact catcgttttt gctactcgtt agttactgca gtagaggatt
     2581 ttttacggct gccggatgcg gcgtgaacgc cctatccgac ctacggttat gttctggctc
     2641 gttggccttt ggcaacgatt atcatatgag attggggcaa ttacgcgccc tcgtcaaatc
     2701 attgcgcttt ttctttacgc ggttggcgca acacttcgtt gatttgcgga tcgtccagcg
     2761 gacccgaaat gtgatagcgc aaaatggaga ctttgctcca cagcggcccc agcactttac
     2821 tggctgcaaa tactgccgcg ccaacaatgg ggttaaccgc aaaagccgca gccacgccca
     2881 ccgtcgcaga aatctctggt gcgacaaccg cttccatatt caggtcgcga cgtaccagat
     2941 ttaccgaccc tttcatggcg atatccgcct ccaggccatc caccagcgtg tcgtcggtgt
     3001 gcataacgcc gtctttaatc cacgcggtgc tgcgaatgga gtcaaaatag aacccttcgc
     3061 caaaagtgtc tctgaaatca aaacgcagct tacgcatcag ggcatcaacg ctcaataagc
     3121 gcagcaattg cccggcatgt ccggtattga tttcggtaat ttcgccttta cccagttgag
     3181 tatgaatgat gccattcaac gtcgcctcat ctggttgcca gggtgctttg cgccagtgta
     3241 aatcgtaatc cacattaaat gacgactggc gtatgggcgt cgtgacacca aaaaattctg
     3301 cggtggcatc aattttctgc ccgcgcagtt ttcctttcag cgaggtacgt tcattccccg
     3361 gattattaac ccattcacca tcggcagtaa gccgcgagaa accagtatca atcagtccat
     3421 tggtcagcgt taacgtatcg ccagaaatgg tgagatcact gtcaatacga ccgaattttt
     3481 gcccccagaa ccagcactct gtgcatcgta tttgtgcgtc cggccagcca cggaagttaa
     3541 tgcgctccgt tgtcgggaaa ggtgatgacg gtgttgaatc accacgagtt ttcgccacgc
     3601 tcgggttgta ataaagatat ttgatattcg ccagccacgg cgcgttatta cgcatcgcca
     3661 gcgtggcatt gatttcacgc ccctgtgcct caaccagggt gccatttgcc gtcggttgcg
     3721 aaacaatact caggttattc cattgctgat ttcccagtga caacataggc gtacgtaacg
     3781 ttatgtgttg tgggaaactc gctgcaccac cgacactctc cgcagcgcct ttctggaaca
     3841 gggccagcca ctcggcacca ttcatcggcg gcatattgag ttcaacgcca ctttgttccg
     3901 gcaacggcgg gagcgtttta ctgtctgccg cccaaatagc acgatcgagc gtcagctttt
     3961 gaccaagcaa ccagcggcta ttgaaatggt tatccgcacc agcctgtccg gttaattcaa
     4021 agctgctgag attgccatca accttaacgt ttaccggcag tggttcaccc gcaggtttgg
     4081 ctaacggtga aggtaagtga ctgctcacat tattcagatc gccgttcagc tctacgttat
     4141 aggtcgcgcc agcatgataa ggcagatcaa tgcccacttt accatcccac gccacgctgc
     4201 cactcaatgc ttcgttcacc gctgcaggca gaacgccggt tttcgccggt tgccagttac
     4261 cgttgaggtt taccgcaacc tggtaggctt ttgccccttc tttggtggaa aaatccacgt
     4321 tcaacggctg attaaaccag cttgctgtca gtggttcact ttgcagatcg ccattgataa
     4381 agctgaattt accgctcaaa tttttcaggg tgctgtcgag tagtttgata aacagactgt
     4441 tattacgcag cgtcacttca cctttcgcgg ttaccagttc gccgttcagc gggatatcaa
     4501 gatgtaagcg agcattcaca tcgccgtcga gctggagttc ttgcagggtc gcacccagag
     4561 aatctttcag cggtgtctca tcaaagtaag ggccaacggc tttacccgga cctttaatgt
     4621 cagcgtcaat cagcagtttt tctttcgagt agtcagggat cactgcggtg agattactcg
     4681 cgcgcacgcc gcccagatta acgtcatcgg ttttcatcca taaaccgtcg ttaataaagt
     4741 ccagttcaat atcaaggtta gttaatgcag gccagtccgg ctggaaggca aacttcgcgt
     4801 tacgcagcgg caccagcact tcaaactgac cttcgttgtg tttatagggg aagagttgcg
     4861 gattgccacc ataaaccagc gtcgcgttat ccgcttcacc gccctgaatc gcgccactta
     4921 agtaatcaac taggtcttta cccatcaagt tttccgggaa atagcgccag gcttgtgaac
     4981 catcatcggt actgatgcca gccagaatac ccagccaggg ttcatcgtta gcaggttgca
     5041 ggtaacgaaa accgccgcgc gcatggacag ctttggcttt aacgtcaata ttacgcccat
     5101 ccaggtggaa acctttgtca ttgttcagcc agcttatagt tgcctggccg tcggcgattt
     5161 ccagtggcgc acggaatacc gtttcgtaag gcatctttgc ctgcttcatc gacgcggtaa
     5221 gcaaaccatt ttcaacgctg ccggaaagcg tcccggagaa gtgttccgca cccggtaata
     5281 atttccattg cttccaggcc agatcgctcc acgatgcctg aaaacgggtc ttgtctgccg
     5341 cctgaagcgg gatatccagc gccagagtgt taatcttgcc gctcggttgt gtggagcgcc
     5401 aaacatcacc cagtgcaggt gaaagtttcg cggccagcgg gcgtatgccc tccaggcctg
     5461 ccagctccag attactggcg cgaatccgga gttcgtcact gcgtttattg tctttgccgc
     5521 caacgtcctg ttccggtatc caggccagcg tcaatgctcc gctcggccag ggtttgccgt
     5581 ccatcgtgat ccgtgtatcg ggaatagaga actgccagcc cggattttca cgcgtaatat
     5641 gcgcggtcag attatccacc gacagcgtat gcgtttgctt ctcgcccaac cagctggcac
     5701 cgccctgttt cagccagacg tcaccgccgg ttacatcgcc tttgtcgatc gtcatccagc
     5761 cttcaaggga gaactgtgcc gtttccagag caatattgtc ctgcatccat ttaccgagcc
     5821 acggcttcag gtcgatgtca tccgcctgta gccagacgcg accattgctt aacaacccct
     5881 catcatcgcg caaatccatg cgcacctgca tcacgccgtg ctgtccggta aggctggaga
     5941 ggcttaccag gccttccgca cggtgtcgac gtggatcgtt cagccaggtg agttgtggga
     6001 tcgccagctc ggcgcgctga ccggatggcg tcagaaaact gacttcactg tcgcggagat
     6061 cgaaatggtc aaattgacga agaaacagat cgctgatgtg actggcttcc aggctatcat
     6121 taccaccacc gctggtgata ggagtgttgg tgcgaaagcg cagctgccag aaagtgaggt
     6181 cgcgaaactg ccagcgcata tgtaacaagc tctgccagac atccagcgcc agagtaacgc
     6241 gtttaaccga aaattcgccg ccatctttta gttctgcacg gatgtcgtgt gcttcaagcg
     6301 tcgggccaaa attctgccag ctggctgaga gctgactggc ttctaccggc atgccagtcg
     6361 cggattctat tttgttgagg atttccggac gccaggcgtc aagatgcggt aaagcaatac
     6421 gcaggccgct aaccagcagg gctgcgatca caacgagcgc ggctccagta agcagtaaaa
     6481 tccccggcaa tcgcctcacc cgtcactcct tgtctgctaa aaatgtgact caaaaaccct
     6541 ttgccggatg gcggcccagc atctgtttac atcattacga cgtcaaactg ctcctggtta
     6601 tagagcggtt caatttgtac tttaacctgt ttgccaacga aaatttccac ttccgccagc
     6661 gagtgtgact cttcgccttt caaggcttca gctactgccg gagaagcata gaccaggaaa
     6721 cggtcggagt cgtaagcatg gtggacacga acaatctcgc gcatgatttc atagcatacc
     6781 gtttccacgg ttttcaccgt tccgcgaccg tggcaggttg ggcattcgtt acacagtacg
     6841 tgctcaatgc tttcgcgggt gcgtttacgc gtcatctcca ccagccccag cgccgaaaaa
     6901 ccattaacgc tggttttcac ccggtctttg ctcaacgcct gctccagcga gtgcagcacg
     6961 cggcggcggt gatcttcatt attcatatcg atgaaatcaa taatgataat cccacccaga
     7021 ttacgcaacc gtaactggcg agcgatagcc tgcgtcgctt caatattggt attgaaaatg
     7081 gtgtcgtcca gattgcgatg accgacaaac gctccggtat tgatgtccac ggtggtcatc
     7141 gcttcggtct ggtcgataat cagataacca ccggatttca gttctacttt gcgttccagc
     7201 gctcgctgga tttcgttttc gacatcaaag agatcgaaaa tcggctggcg tccggtgtaa
     7261 tgctccagct tgctggtcat ctcgggaatg tactccgagg taaactccag caatgcttcg
     7321 taagtcaggc gtgagtcaac gcgaatgcgg tccagttcgg cgtcggcgaa atcacgcaga
     7381 acacgctgcg ccagcgccag ttcgccgtac agctgataac gggtctgcgg gcgtttttta
     7441 cgctccataa ctttggtcca gacgcgtttc agataagcgg catcggaggc cagttcagcc
     7501 tcgccaaccc cttccgctgc ggtacggatg ataaacccgc cctgctcgtc gcaatactct
     7561 gcgaccactt ttttcaggcg ttcacgttct gattcgcttt caatacgttg ggaaacccca
     7621 acgtgagaag cccctggcat aaacaccaga tagcgagaag gcagcgtgat atcggtggtc
     7681 aggcgcgcac ctttagtgcc aagcggatct ttcaccacct gcaccatcag atcttgcccc
     7741 tgacgaacca gttccgagat gtcgcgcacc gtgaattgct tttgttcttc acccgccaca
     7801 cattcggtgt gcggcatgat atcggatgca tgaagaaacg cggctttatc cagcccaata
     7861 tctacaaaag ccgcctgcat acccggaagt acacgactta cacgaccctt gtagatattg
     7921 cctactattc cgcgtcgcgc ctcacgttca atatgaattt cctgcagaat accgccatca
     7981 atatacgcca ctcgcgtttc cgaaggcgtt acgtttacta acaattcagc cgtcatgttt
     8041 atcccttttc tcacgcagtg cgttaaaatt acttaataat tcatacgttt caaccagcgg
     8101 taagccgact acggcgtgat agctgccatt tatcttcctg acaaaacagc cacccagccc
     8161 ctgaataccg tatgcacctg ctttatctaa cggttcaccg ctggcgacat agcccgcgat
     8221 gtcttcgtct gttaacgttc tgaaagtcac atcggtgacc accaggcaat cgagaatgtg
     8281 ctggctgtcg gccaacacca ctgccgtcat cacctgatgg gtctgacccg ataatttgcg
     8341 caacatctgc gccgcatgct ctgcgtcgcg cggtttctcc agcacttctc cattcaggat
     8401 aacgatagta tccgcaccca gcaccgggag atcctgcgcc gtttgcgcga cacccgcctg
     8461 tgctttcttg tgtgccagac gcacaacata ctgctgcgcg ctctcctgcg gctgacgctg
     8521 ctcctcaatg cccgtaacaa tacgttcaaa ggtcacgcca agttgcgcaa gtaactcctg
     8581 acgacgcgga gaaccggaag ctaaatacag agaagtcata gaaaccttta ttgcactgca
     8641 aactgctgac ggactttacg catcagcaag aaaatccacg gccagagcac cccattgact
     8701 acactactcc agaacacttc cggtctgaaa gagacgttaa tcactaaaaa ctctgcccag
     8761 aaaacaataa tatccaccac cagcgaaagc aacatgacga ctagcgcctg ctgccataat
     8821 gcgaggttgc ggaaaagctg gtatttcagc gccaccaggt aagcaatgat gctcatcgcc
     8881 aatacgcgta cgccaagcgt cgagccgctg atcagatcca gtatggcacc catcacaaaa
     8941 cctgtgccca catttacgcg atggggcaag gccaggatcc aatacaacaa gataagtaac
     9001 acccagtttg gccggaaaac aatcaggtta tccggccagg gcatgatttg cagcaacagc
     9061 gcaatgagga aagagagcca gattacccaa cgtccctggc tacgatagct cgccactatt
     9121 gccctcccgg cgcacgcgca ggcggttgag caccactttg cggcggcgta gccctttgtg
     9181 gagagcgatt agcagcaggc gcagtagctg catttcctgt cgtcggttgc tgcggagtcg
     9241 gctgagcgat ccccgttgtc ggttcaggta actttggccc catcgcgtct ggcgaaggca
     9301 atacctgcgg catcatctgc atcagacgtt cattagcaac acgatgcacc tcttccggcg
     9361 tcatcgggtt agcgccgtta cgatccgcgc cccacagcag cagcagataa cgcaaacgtt
     9421 gcagtcctgc agtcggacgc gcctgaatca cagtataagc gcgctgggta tcgagtttta
     9481 cggaagagac aaccgcgacc ggatagcctt ccgggaaacg accgcccaga ccggaggtca
     9541 ccagcacatc accaacacga atatccgtat tcgctggcag atgctcaagc tgcaaatcat
     9601 ctgtacaacc gttaccggct gcaattacgc ggatatcgtt gcgcagcacc tggattggca
     9661 gcgcgtgggt cgcatcacaa atcagcagca cgcgactggt cagtttagcg acggccacca
     9721 cctgaccaac aacacctttg tcgctgatga ccggctggcc ttcataaacg ccattaacgc
     9781 tacctttatc gataacaact tgatcgctat aaggatcgtt aaccgtggag ataacctgag
     9841 tcaccatttt ctgctcatcc tgacgcagcg gggaacccag cagctcgcgc agacgcgcgt
     9901 tctcctgttt gtattgtcca agcatcagca gttcactgtt tttcagcaac agttcctgac
     9961 gtaacgcccg gttttcaagt tctagttggt cacgcgaggc cagcgtctgc gatacgccat
    10021 ccagcaattc acgaggagca ttggaaacaa agtagaaagg actgacggcg gtatccatat
    10081 aagtacggat ttgactgaac gtccccaggc ggctgtcggc aataataatg ccgagcgcca
    10141 ccagcaccgc cagaataagg cgaatctgta gcgacgggcc acggctaaaa attggcttca
    10201 taagttatgc gtattctcgt atcagaccag gcagggtaaa cagacatttc ccctgcctgc
    10261 atccgattac tcttcgctga acaggtcgcc gccgtgcatg tcgatcattt ccagcgcttt
    10321 gccgccaccg cgcgccacac aggtcagcgg gtcttcagca acaacgactg gaatgccggt
    10381 ttcttccatt aacaaacggt caaggttacg cagcagtgcg ccaccaccgg tgagcaccat
    10441 gccgcgctcg gagatgtcgg aagccagttc cggcgggcac tgttccagtg caaccattac
    10501 cgcgctcaca ataccggtca gcggttcctg cagtgcttcg aggatttcgt tggagttcag
    10561 ggtaaaaccg cgtggaacac cttctgccag gttacggcca cgaacttcga tttcacggac
    10621 ttcatcgccc gggtaagccg aaccgatttc gtgcttgata cgttctgcgg tggcttcacc
    10681 gatcagagaa ccgtaattac gacgcacata gttgatgata gcttcgtcga aacgatcacc
    10741 accaatgcgc acggaagagg agtaaaccac gccgttcaag gagataacag caacttcagt
    10801 ggtaccacca ccgatatcaa ccaccataga accggtcgct tcagaaaccg gcaggccagc
    10861 accaattgcg gcagccatcg gttcttcaat caggaagact tcacgggcac cagcgccctg
    10921 cgcggattca cgaattgcgc ggcgttcaac ctgggtcgcg ccaaccggta cacaaaccag
    10981 aacgcgcggg cttggacgca taaagttgtt gctgtgcact tgtttgatga agtgctggag
    11041 cattttttca gtcacgaaga agtcggcgat aacgccgtct ttcattgggc gaatggcagc
    11101 aatattgccc ggcgtacggc ccagcatctg cttcgcgtca tgacctactg cagctacgct
    11161 tttcggtgaa ccggcacgat cctgacgaat ggccaccacg gaaggctcat tcaatacgat
    11221 gccttgtcct tttacataaa tgagggtatt cgcagtaccc aggtcaatgg acaagtcatt
    11281 ggaaaacatg ccacgaaatt ttttcaacat actaagggat aatcctgaaa gctggggcgg
    11341 aaaagaaaat ccgcttactt taccaaccac acgcagcagc gacaaggcgc aaaaatcatc
    11401 tgctacggtg aaaattagtg cagttcgttt cctttgttac aaatctctgc ctgagtccag
    11461 aaaggcttaa tgcatcagca gcattcctcg cctgtttgca accgcgtaag gtcattcatc
    11521 tgcatatgtg ctgcaacaat ctggcgagca gacaagcaca ctcccatgag acgcagcgcg
    11581 cgttattcta cgtgaaaacg gattaaacgg caggttaaac cgagtatctt tgtgaatatt
    11641 ttttcacgtt agtatcaagt ggctgtgagg acgcgaaaaa atccccttgc ccgcctgtaa
    11701 caccgcgctg aatcagggtc tgccactcgc ttcgcgaacg cacaccggtg gcgtaaacct
    11761 gggtgctggt cccggagcag gcttccacca ggctttgaac cagcagctgg ttctccgttc
    11821 gcttctcaat gtttctgacc agccccggat ggagcttgag taactcaaca ttaagttctt
    11881 tgatccaact ggtgcttacc agcgtcaaac cagcctggtt gacggctacc cgtaccccta
    11941 aagcattcac taaacgaata acaggttgta aacgactgat atgttgacct acatcggcct
    12001 ctgcaagttc aataattatg cgttttcgtt gtgatttttc acattgcatt aacgtatcgc
    12061 gcagccaacg ctgaaaacgc gggcgaatca gcgactcaac ggtaacctga atcgccagat
    12121 tttcctctgg ccagtaacgc aatagtggaa taagacggct gatttgcaga cggtcatact
    12181 cttccgataa gccaaactgc aagaccatcg gcatatactc cgccgagcta acctcttcat
    12241 taccatcgaa gatgcggcac atgagttcgc gatgatgaac ctgaccttcg cgagtaaccg
    12301 ccggtttttg ataaaggcgc gggccgccgc gactgagcat ttgctcgata agcgtacgcc
    12361 agcgaacatt accgcgtcct ttttcaggca acgagtcatc gtaaatagcc cagctattgc
    12421 cgccctgcaa tcccgcatta cgcgtggcag actctgcatg ttccattacc tgctcggtat
    12481 cctgaccact acgccaggcg cagataccaa tgtggatcat atcgtcgcga tcgagcattt
    12541 tattgttcgg caaggtatcg acggctttga ttaactgacc ggcgatgctc tctgcctctt
    12601 ttaacgtccg gtgcggtaac agcgcagcaa aatcactgcg gtggtaacgc gccagcagtg
    12661 cgccagggta gcgcatcata aatgtcgaca gcagattcgt cagagtgaag aactgttctt
    12721 caacctggct gtgcccccaa gtatcgctca acatattgaa atccggcaga cgaatcatca
    12781 tcacgatccc gtgggtacct actttctcct gatcttccag taacgttgct aactgattat
    12841 cgaaaaagag tcggttattg aggccggttt tcacgtcctg ggcggcataa gagcggatca
    12901 gcgtatcaag acggctgtgt tgttcgcgtg cgttctgaat ttcacgaagc agcgtatcca
    12961 gcgcactgct ggttctgggc ggccattcat agatggttcc caacacatta gagccacgct
    13021 caccgtttaa gatacgagta gcccgggttt ccagcaattc ttgcccggca agttgccgtt
    13081 gtaaccagcg taccgccaag aagagcataa caatgataaa gctaatcgcc cccgtgagcg
    13141 gcgcggtggt catcaacgaa tggaaatagt tgcccatcgg atcctgataa accagacgca
    13201 gcgacatccc cggatacttt atcaacggaa cgctcagttc acgaaacaga tcgctggagc
    13261 caaccggacg ataactacca tttctggcca gggtataaac ctgtttatca ccatggagca
    13321 ggtctacacg aacgatatct gccgacatca ttaattcggt aatttgtggc cttaatgtgc
    13381 tgaagtcatt cgacacaagg tgggtatcaa tcgccgtcgc caccgcctga acgcgatgac
    13441 taaacttata ctgaattgcg ttgtagaaac ttagcgaaca gcccagcaaa gtcacaaaaa
    13501 ttgttaaccc ggtgagcagc gtaacaaagg ccgaaaattt cgtcgttaat ctcatccttg
    13561 tgttaactcc gatagtgagg aagcgggcat actagcaaat cagatttatc tcgcaattta
    13621 ttgcgcttca tcggctttgc ttttccatta gcgagtataa tcttcagaaa ttattttcca
    13681 atccatcatg cacatgagga ccacttatgc aggcgttact tttagaacag caggacggca
    13741 aaactctcgc atcagtacag actctggacg aaagtcgcct gccggagggc gatgtcatgg
    13801 tcgatgttca ctggtcgagc ctgaactata aagatgcgct ggcgattacc ggtaagggaa
    13861 aaatcatccg taattttccg atgattcctg ggatcgattt tgccggaact gtacgcacca
    13921 gcgaagatcc gcgttttcat gccggtcagg aggtgttact cactggctgg ggcgttggtg
    13981 aaaaccactg gggtgggctg gcggagcagg cgcgagtgaa aggtgactgg ctggttgcca
    14041 tgccgcaagg gctggacgcg cgtaaagcaa taattatcgg tactgccggt tttaccgcca
    14101 tgctgtgtgt gatggcgctg gaagatgccg gtgttcgccc gcaggacggg gagattgtcg
    14161 tgacgggtgc cagtggtggc gtcggcagta ccgccgtggc gctgctgcat aagttgggtt
    14221 atcaggtcgt tgccgtttcc ggtcgcgaaa gtacccatga atatctgaaa agtttaggtg
    14281 ccagccgtat tctccctcgt gatgagtttg ccgaatcccg tcctctggaa aaacaagtct
    14341 gggctggggc aattgacact gttggcgaca aagtgctggc aaaagtgctg gcgcaaatga
    14401 attacggcgg ctgcgtggcg gcctgtggtc tggcgggtgg ttttactctg ccaaccacgg
    14461 tcatgccatt tattctgcgt aatgtccgtt tgcaaggggt ggattcagta atgacgccac
    14521 cagaacgccg cgcacaagcc tggcagcgac tggtcgccga tttaccggaa tcattctata
    14581 cccaggcggc aaaagagata tctctgtcag aggcaccgaa ctttgccgag gccatcatta
    14641 ataaccagat ccagggtcgc acgctggtga aggttaacta accatttagc aaggaataat
    14701 aagagaggga actcattttg acatcattat tcaatcggtt aacgggaaaa gcggttagcc
    14761 ggacagcttt cgtcgaacac cttggtcagg aagttgtaca acatcatcca aactggaaag
    14821 tcatgatttc gactgaccac aaattgatgc gcattgatac tcctctaaac agccattatt
    14881 gatacgcctc cgtcgcctgt taggtttatg gtgctttgcc tgggcgacac tacgcttaac
    14941 ctgttactga tttctggtac catggggtga ataatctgat tttatttgac tacaaattaa
    15001 ccactcgaac ctatttaatg ctgagaattg tcagtcggtt gattttgcgt gctttagcat
    15061 tcacatctat ccagacgatg cagtgaaaat tgggtaatcc ccagcaaccg ctgcgtaatg
    15121 tcgtctatct tgtcgcgatc ctggcatccc tacattattt gtggtctgtg aagattatct
    15181 cattgcagcc cctcatcttc gcagggctgg ctttacagct tttcacctta cgttataaga
    15241 agttccgtcg atgatggcgc taatttcgtg aattgtgcgg cttgttgcaa attacacggt
    15301 gttgaaggtt atttacatgt tagctgttga ttatcttccc tgataagacc agtatttagc
    15361 tgccaattgc tacgaaatcg ttataatgtg cgacctcgtc ctccctgacg cagtttttgc
    15421 gctgcggaaa aggtgacatt ggcgcaacga aggtatattt tgttttttgc cggaggatag
    15481 cagcagatcg ctgcacaatg tccgtcaagt ctaacattga tactctgggg caaaatagac
    15541 cggcgtcccg gcctgctgga atttatcgct atgcatacag ctgtcggggc atacgcttta
    15601 cagacggcgg tgaaacgcct gtcacaatca cactaaacaa agagtacgga acccactcat
    15661 ggatattcgt aagattaaaa aactgatcga gctggttgaa gaatcaggca tctccgaact
    15721 ggaaatttct gaaggcgaag agtcagtacg cattagccgt gcagctcctg ccgcaagttt
    15781 ccctgtgatg caacaagctt acgctgcacc aatgatgcag cagccagctc aatctaacgc
    15841 agccgctccg gcgaccgttc cttccatgga agcgccagca gcagcggaaa tcagtggtca
    15901 catcgtacgc tccccgatgg ttggtacttt ctaccgcacc ccaagcccgg acgcaaaagc
    15961 gttcatcgaa gtgggtcaga aagtcaacgt gggcgatacc ctgtgcatcg ttgaagccat
    16021 gaaaatgatg aaccagatcg aagcggacaa atccggtacc gtgaaagcaa ttctggtcga
    16081 aagtggacaa ccggtagaat ttgacgagcc gctggtcgtc atcgagtaac gaggcgaaca
    16141 tgctggataa aattgttatt gccaaccgcg gcgagattgc attgcgtatt cttcgtgcct
    16201 gtaaagaact gggcatcaag actgtcgctg tgcactccag cgcggatcgc gatctaaaac
    16261 acgtattact ggcagatgaa acggtctgta ttggccctgc tccgtcagta aaaagttatc
    16321 tgaacatccc ggcaatcatc agcgccgctg aaatcaccgg cgcagtagca atccatccgg
    16381 gttacggctt cctctccgag aacgccaact ttgccgagca ggttgaacgc tccggcttta
    16441 tcttcattgg cccgaaagca gaaaccattc gcctgatggg cgacaaagta tccgcaatcg
    16501 cggcgatgaa aaaagcgggc gtcccttgcg taccgggttc tgacggcccg ctgggcgacg
    16561 atatggataa aaaccgtgcc attgctaaac gcattggtta tccggtgatt atcaaagcct
    16621 ccggcggcgg tggtggtcgc ggtatgcgcg tagtgcgcgg cgacgctgaa ctggcacaat
    16681 ccatctccat gacccgtgcg gaagcgaaag ctgctttcag caacgatatg gtttacatgg
    16741 agaaatacct ggaaaatcct cgccacgtcg agattcaggt actggctgac ggtcagggca
    16801 acgctatcta tctggcggaa cgtgactgct ccatgcagcg ccgccaccag aaagtggtcg
    16861 aagaagcacc agcaccgggc attaccccgg aactacgtcg ctacatcggc gaacgttgcg
    16921 ctaaagcgtg tgttgatatc ggctatcgcg gtgcaggtac tttcgagttc ctgttcgaaa
    16981 acggcgagtt ctatttcatc gaaatgaaca cccgtattca ggtagaacac ccggttacag
    17041 aaatgatcac cggcgttgac ctgatcaaag aacagctgcg tatcgctgcc ggtcaaccgc
    17101 tgtcgatcaa gcaagaagaa gttcacgttc gcggccatgc ggtggaatgt cgtatcaacg
    17161 ccgaagatcc gaacaccttc ctgccaagcc caggcaaaat cacccgtttc cacgcacctg
    17221 gcggttttgg agtgcgttgg gagtctcata tctacgcggg ctacaccgta ccgccgtact
    17281 atgactcaat gatcggtaag ctgatttgct acggtgaaaa ccgtgacgtg gcgattgctc
    17341 gcatgaagaa tgcgctgcag gagctgatca tcgacggtat caaaaccaac gttgatctgc
    17401 agatccgcat catgaatgac gagaacttcc agcatggtgg cactaacatc cactatctgg
    17461 agaaaaaact cggtcttcag gaaaaataat ttgtgacgcg cggtgttttc atcgcgctta
    17521 gcaaagaacg taatcaacgc cgggcctgtt aacgctgccc ggcgttttta ttgccgttag
    17581 ctaacggtag cctgcaattt ccgctttcaa cgcgctcatt tcagcatcgc catcaacaat
    17641 tcccagctca aagcagtatt tctttgtttc ccctggctca ataaagggca gacgcccctg
    17701 cgctcgttct ttatcacgac cgtcaggaaa actattactg acctccagtc ccataacata
    17761 ctcacccgcg cctaaatttt tccactggat aaagttgggt agctgcatgg tgtcgaattt
    17821 ctcgtaaatt ccccacccca gttcggcgtt caccagagca actttactca tcccctgact
    17881 gtcgctattc aacgtgagat agtaaacctg ctcgtcaaaa ccgggctgtg gggtacaaat
    17941 ctccagatga ctggttaacc cttccgccgc atgcggagtt cgtggcgtta cgcttttagc
    18001 cgacagcaag atttctggcc tgtggcgata ataaaggcca gccgtaatta aagtgataaa
    18061 gaatttgcag cggctggcgg gtgaaaccat gattagtcac cttgtcttca atgcgcagca
    18121 cattttcgcc ataacggcag cgaatggtcc tttccagcgt cagattttcg ccgaacagtc
    18181 gcgtttcccg tactttgcca ttgatgacaa attcaatgcc gtcgtcggtc cgttctgtgc
    18241 gataccccac ctcttctgct ggagtcgcag ccaaacgccc gtgtaacccc agcgtttctc
    18301 cttcatcctc acaaggcgtg ccaatgtagc taagtccgca ggtagtcagt aaccccgcgt
    18361 aaaagctacg taaaaatccg ttgccctgag actcaaaaaa agcgggggcg acaatgccgt
    18421 tgggggtgat aaaagaaagc gccgtgtcct tgtagctggc ccatgcaata tccagcgcgc
    18481 gatcttttac caccgtaaat gccagtcccg caccagtacg gacatcaaac gcttctgtac
    18541 cctttgctct gccactacgt aactcatagt gtttaattcc ggcaacctgc gagagatcgc
    18601 caatccacga ccaaatttcg tttttcattg tgctgcttcc cgaatggaca tgtcatggcg
    18661 ctggatgaac gcttcgattt ccgcaaggcg agacgttccc tccattgggc cacgtctggt
    18721 taccgccagc gcaccgcagg cattggcata ttgcagcgct cgatggacgt caaaccctag
    18781 ctggcggcag gcaatccacg cgccaccaaa gcagtctccc gcaccggtcg ggtcaacttc
    18841 ttccaccgga tagctctcaa catgaaactg ctcattcgcg gagtagtaac tggcaccttg
    18901 attgccgcgc ttcacaatca cctctttaac accttcttca agaaaacctg cgattgcacg
    18961 ctccggcgtc gaatgtggcg acaagagcaa cacttcgccc tcgctgggca tatagatatc
    19021 cgttaactca agaacaaagt ggagggcgtc acgcatttct ggaatatcga gcatctcttt
    19081 acgaatatta gggtcgaaag aaataacacc accatttgct ttaacgatag ttaccgcttt
    19141 ttttactgcg tctaccatat gaaacgaaaa cagggaagag cccataatat gaaaatgggt
    19201 acagtccttc agaatatttt catcaacatg ctgcgccgat aatttaccgc aagcggcatt
    19261 cttaatatta aaaataaaat cgcgatcgcc agagttatga taagtaacaa aggcactccc
    19321 ggtagcttcc tgaggtaaga ctgaaatccc cctgatatcc acaccgtcag cagccagacg
    19381 atgtatatta atatcaccaa agccatcatt ccccacacag ctaatgatgc cgcacggaac
    19441 gccaagacgt gtcacctggt cgataaaaat agcgggcgca ccactgggat aagggccgtt
    19501 ccagatacct ggttgatcaa atggctggcc gattttactc gccataattt caaccagaat
    19561 ttctccaatt gtgaagacct tactcatatg attaattcct tgaaatgatt agctgcgttt
    19621 tcttttattt cttacgtcaa aataaacggc tacaagaatg actaatcctt taaccaccat
    19681 ctggtaaaaa tagggaacat tcattaagtt catgccatta ttaataacgc cgatagttaa
    19741 tgcgccgatc accgtcccca ccacggttcc atagccgccg ttcaggctgg tgcctcccag
    19801 tacagccgca gcaatagcat ccagttcgta gctcaacgcg gaattcggtt acccggaata
    19861 gaggcgtgag gcgaggatca tcccgctgat acctgccagc acggctgtaa tcataaagac
    19921 tttaattttc aagcgggtga catttatccc tgaataaatt gccgcttctt tattaccacc
    19981 agtgatataa atttttcgtc caaaggttgt tttagtaaga atgaagtggt taatcagcaa
    20041 taagatgatt aatatccaga ttggaatagg tatatagaga aattctccat tccccagtgc
    20101 cagaaaggca tcatctttaa tcatgatggg cataccatcg gtcactatat aagcgatacc
    20161 acggaagatc cccatagtag caacggtgac aataaacgac gggatccccg ctatagccgt
    20221 caagccacca tttatcatcc ccagagccag tgctgcaata atggataatg ggaccaataa
    20281 aataaatgga attccccatg aagccccaag cgcagcaaat gtgccaatca atgcaatggt
    20341 tgaaccgacc gataaatcaa tatcgccaag caaaagtaca taagtcattc cataggcggt
    20401 aattgcaatt atcgaaacct gaagaataat attagtaatg ttgttggcgg taaaaaaact
    20461 gtcattaata aaactgaaca gaatgattaa caccaccatt ccactgagga taccgccata
    20521 ttgcctgaag acttttttaa aattatttgc aatgggaatt tccgaaggtt tcggactggc
    20581 aagagttttc tctgtcattt aacgtactcc tgttgcagcg gtcattacct gtgtctgcgt
    20641 taaattttca catgaataaa tatcggcaat cgttttatta cgcattacca ccagacgatc
    20701 gctaagtgcc atgacttctg gcagttcaga ggacaccaga ataattgctg tccctgccgc
    20761 agccagttga cgtatcacgt tatagatttc aaattttgcg ccgacatcca ctccgcgcgt
    20821 tggctcatcg agcaataaaa tacgcggagt tttctccagc cacttcgata acacgatttt
    20881 ctgttgatta ccgccgctca gcgctcccac tgcctgatgt acagaactca ctttagtgtt
    20941 cattctggca attgattgca gcgtcttcgc ctcttcttgt cggtgattga tgagtcccat
    21001 ccgcgaagcg ttatattgaa acgccacatg gtgcgtgttc atattgacgt cgtgcatcag
    21061 cactaaccct tcttccttgc ggttttcggt cacaaaagca atgccatgat caatagcctg
    21121 tttaggatta gtgatcctga cttccttacc atcaatcaac acagtcccct gatacgaaac
    21181 gaggccaaag agcgccttca tcacatcgga acgcccggcc cccaccaggc caaaaaatcc
    21241 gaggacctcc cccggacgca cagcgaaact gatctcttta aaaagcgaag gatgggaaag
    21301 attttttact tccagttttg caggaacatt ttcatccggt atttccccaa gacgtgctgg
    21361 ccaaatattt ttcatttccc gaccaaccat ttgggcaatc aaatgctgga tatcactgtt
    21421 tttcttttca tgcgtggcga tatactcacc gtcgcggatc accgaaatat catctgaaat
    21481 ccgcataatt tcatccatgc gatgagagat ataaataacc gcacagcctc gctgctttaa
    21541 tttttcaata atgttaaaca gaatctccgc ttcattatca cttagcgaag atgtcggttc
    21601 atccataatc acgacatccg ctgcatagct taaggcttta gcaatttcta ccagttgcct
    21661 atgtgccatc gacatattgc cgaccatttc tagtgggtct agtggtatgc cccgttccgc
    21721 gaggagattc tggcaatctg cgagcatttt tttatcgtta ataatgccaa aacgtcgcgg
    21781 ttcattattg gcataaatat tttccgccac ggtcatatta ttactcaggc tcaattcctg
    21841 aaaaataatc gcaattccta actggcgtga atgctgcgga ttattaatgg tgatagtttc
    21901 accacgtagt gttatcgttc cctgggttgg cggataaaca ccggccagta ttttcattaa
    21961 tgtcgattta ccggcaccat tctcgcctaa taaagtatgc acccgccctt tacgtaaaga
    22021 gagggtaata tcttttaatg cttgaacatg accaaatgtc ttcgcaatat gcgaaacatc
    22081 aagaataaca tccgtcataa tgacctcggt gtcacaacaa aacgccaata acgttttgtt
    22141 gtgaggtgaa gatgtttact atttacctgt ataaacgccg ggttgtaccg ggatcatttt
    22201 ttctaccggt ttaccattga ggtgatcaac agcggcatca atggctttag cacccatttg
    22261 atcgggatat tgccgaatca cggccgcaaa cgttttttca ctgtcaacgg catcacgcgc
    22321 ttctttcatg ccatcaaaac caatgatttt gatgttgtcg ttatgcgcag attttgctgc
    22381 gataaccgct gccagcgccg catcatcacc aaagccaaac agaccattca ggttggcatt
    22441 tgcctgcata atgttttgcg cagtcgttaa cgcttcagcc cgcgtaatac taggctgttc
    22501 agcaacaatt ttaatatccg gtgtatcgct taaccctttc ttaaaaccat caacgcgcgc
    22561 aaccaccgat tgtagtgccg gataagaaat aatggc
//
LOCUS       DAJVTY010000055        22472 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVTY010000055 DAJVTY010000000
VERSION     DAJVTY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22472
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.055"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..257)
                     /locus_tag="OQQ66_001898"
     CDS             complement(<1..257)
                     /locus_tag="OQQ66_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1904849.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDC"
     gene            complement(248..718)
                     /locus_tag="OQQ66_001899"
     CDS             complement(248..718)
                     /locus_tag="OQQ66_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1904850.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(903..1697)
                     /gene="thyA"
                     /locus_tag="OQQ66_001900"
     CDS             complement(903..1697)
                     /gene="thyA"
                     /locus_tag="OQQ66_001900"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1904851.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="OQQ66_001901"
     CDS             complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="OQQ66_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1904852.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="OQQ66_001902"
     CDS             complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="OQQ66_001902"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1904853.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="OQQ66_001903"
     CDS             complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="OQQ66_001903"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1904854.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            6204..6893
                     /gene="mutH"
                     /locus_tag="OQQ66_001904"
     CDS             6204..6893
                     /gene="mutH"
                     /locus_tag="OQQ66_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1904855.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6962..7675
                     /locus_tag="OQQ66_001905"
     CDS             6962..7675
                     /locus_tag="OQQ66_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1904856.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7813..8031
                     /gene="ygdR"
                     /locus_tag="OQQ66_001906"
     CDS             7813..8031
                     /gene="ygdR"
                     /locus_tag="OQQ66_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1904857.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8139..9179
                     /locus_tag="OQQ66_001907"
     CDS             8139..9179
                     /locus_tag="OQQ66_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1904858.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="OQQ66_001908"
     CDS             complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="OQQ66_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1904859.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNSTPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10397..12556)
                     /gene="aas"
                     /locus_tag="OQQ66_001909"
     CDS             complement(10397..12556)
                     /gene="aas"
                     /locus_tag="OQQ66_001909"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1904860.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12945..13976
                     /gene="galR"
                     /locus_tag="OQQ66_001910"
     CDS             12945..13976
                     /gene="galR"
                     /locus_tag="OQQ66_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1904861.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="OQQ66_001911"
     CDS             complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="OQQ66_001911"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1904862.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15367..16302
                     /gene="lysR"
                     /locus_tag="OQQ66_001912"
     CDS             15367..16302
                     /gene="lysR"
                     /locus_tag="OQQ66_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1904863.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     MIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="OQQ66_001913"
     CDS             complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="OQQ66_001913"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1904864.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17110..18525)
                     /gene="araE"
                     /locus_tag="OQQ66_001914"
                     /pseudo
     CDS             complement(17110..18525)
                     /gene="araE"
                     /locus_tag="OQQ66_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="OQQ66_001915"
     CDS             complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="OQQ66_001915"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1904865.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="OQQ66_001916"
     CDS             complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="OQQ66_001916"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1904866.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(20754..21935)
                     /locus_tag="OQQ66_001917"
     CDS             complement(20754..21935)
                     /locus_tag="OQQ66_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1904867.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22190..>22472
                     /locus_tag="OQQ66_001918"
     CDS             22190..>22472
                     /locus_tag="OQQ66_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1904868.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYF"
BASE COUNT         5769 a         6025 c         5412 g         5266 t
ORIGIN      
        1 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
       61 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
      121 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
      181 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
      241 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
      301 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
      361 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
      421 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
      481 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
      541 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
      601 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
      661 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
      721 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
      781 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
      841 tttaatggag ggtaaaaaaa accgacgcac actagcgtcg gctctggcag gatgtttcgt
      901 aattagatag ccaccggcgc tttaatgccc ggatgcggag cgtagccttc aatctcaaaa
      961 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
     1021 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
     1081 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
     1141 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
     1201 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
     1261 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
     1321 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
     1381 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
     1441 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
     1501 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
     1561 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
     1621 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
     1681 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
     1741 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
     1801 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
     1861 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
     1921 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
     1981 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
     2041 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
     2101 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
     2161 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
     2221 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
     2281 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
     2341 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
     2401 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
     2461 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
     2521 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
     2581 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
     2641 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
     2701 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
     2761 ccgcgacgct ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc
     2821 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
     2881 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
     2941 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat accatgtatt
     3001 tccgcttccc gggcgatcat tgccagagct cgtaacattg caggatgaag gctgtcataa
     3061 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
     3121 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
     3181 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
     3241 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
     3301 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
     3361 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
     3421 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
     3481 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
     3541 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
     3601 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
     3661 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
     3721 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtatc
     3781 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
     3841 agcaccgaag gctgaatatc cgcgcccatc acggtaggga gccccagcgc acgtaccatg
     3901 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
     3961 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
     4021 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
     4081 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
     4141 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
     4201 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
     4261 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
     4321 gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag cgttgatgcc
     4381 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
     4441 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
     4501 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
     4561 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
     4621 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
     4681 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
     4741 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
     4801 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
     4861 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
     4921 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
     4981 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
     5041 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
     5101 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
     5161 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
     5221 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
     5281 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
     5341 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
     5401 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
     5461 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
     5521 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
     5581 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
     5641 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
     5701 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
     5761 taggcatttt aaaatattca ttataagtca atgcattaca tgtaatttga gtaatgaaaa
     5821 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
     5881 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
     5941 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
     6001 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
     6061 cctattgcag tgctgcgcaa ctcgattatc ggcagcctaa aaagcaatat aaatcagaga
     6121 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggtacataa ttgctgtttg
     6181 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
     6241 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
     6301 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
     6361 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
     6421 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
     6481 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccacg
     6541 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
     6601 tggcgcagcg tcgcgtagga tccccgttgc tgtggacccc gaatgaagag gaagaccgac
     6661 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
     6721 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
     6781 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
     6841 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
     6901 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
     6961 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
     7021 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
     7081 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
     7141 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
     7201 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
     7261 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
     7321 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
     7381 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
     7441 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
     7501 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
     7561 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
     7621 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
     7681 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
     7741 ctgctttcaa gatatataac gtcgttttat aaacagacta ttatcatagg taggccaaat
     7801 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
     7861 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
     7921 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
     7981 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
     8041 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
     8101 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
     8161 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
     8221 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
     8281 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
     8341 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
     8401 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
     8461 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc
     8521 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
     8581 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
     8641 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
     8701 gctacctgca tctggcggac aaacacgatc tgccgagtat tgtcaccatt cagaaccctt
     8761 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
     8821 tcgaactgct ggcctattca tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
     8881 gtgcaaaaac cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
     8941 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc
     9001 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
     9061 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
     9121 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
     9181 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     9241 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     9301 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     9361 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     9421 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
     9481 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
     9541 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
     9601 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagagt tatcggtaat
     9661 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     9721 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
     9781 ggcattcagg aaactgcggg tcatgttgat gagattccaa gactgccccg gacgcgccgc
     9841 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     9901 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
     9961 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    10021 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacccccac
    10081 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    10141 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    10201 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    10261 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    10321 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    10381 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    10441 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    10501 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgagcg
    10561 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    10621 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    10681 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    10741 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    10801 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    10861 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    10921 acaacagacg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    10981 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    11041 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    11101 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    11161 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    11221 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    11281 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    11341 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    11401 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    11461 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    11521 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    11581 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    11641 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    11701 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    11761 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    11821 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    11881 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    11941 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    12001 cagcgatttt gcgacggtca cgggcacgcg acgcatccgg catcgccacc tgcgttggag
    12061 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    12121 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    12181 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    12241 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    12301 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    12361 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    12421 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
    12481 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    12541 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    12601 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    12661 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    12721 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    12781 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    12841 cgtttggctc acattcccac gatgaaaaca cgccactcct tgaaccaacg ggcgttttcc
    12901 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    12961 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    13021 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    13081 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    13141 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    13201 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    13261 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    13321 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    13381 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    13441 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    13501 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    13561 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    13621 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    13681 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    13741 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    13801 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    13861 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    13921 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    13981 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    14041 gcctgaccat tatcaaacag aacttctggt aacagcgaac ggctattgta gttggatgac
    14101 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    14161 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    14221 cataacggtc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    14281 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    14341 aacccggcat caaccagcac aaagtggcgg ctccccattt gtttgacgct ccgcacctga
    14401 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    14461 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    14521 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    14581 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    14641 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    14701 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    14761 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    14821 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    14881 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    14941 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    15001 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    15061 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    15121 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    15181 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    15241 ggcataacaa actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa
    15301 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    15361 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    15421 ggaagcctga ctgaggcggc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    15481 cttgcgcgct ttgagaagat gatcgggctg aaattgtttg agcgcgtacg tgggcgatta
    15541 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    15601 gatcgaattg tcagcgctgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    15661 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    15721 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    15781 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    15841 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    15901 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    15961 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    16021 cgcatgatcg tagaaaccca cagcgccgcg tcaatctgcg caatggtacg ggcgggggta
    16081 ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggtg
    16141 cggcggttca gtattgcggt tccgttcaca gtcagcctga tccgccccct gcaccgcccg
    16201 tcatcagcgc tggtgcaggc atttagtgag catttacaag cggggttacc gaaactggtc
    16261 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    16321 catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    16381 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    16441 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    16501 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    16561 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    16621 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    16681 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    16741 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    16801 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    16861 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    16921 aacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    16981 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    17041 caacggccaa gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    17101 cccgtgaaat cagatgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    17161 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc
    17221 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctgt cgagcagcgt
    17281 caggaaggtc gccgataatc atattcgaca cccagttcgt ggtggtcgaa caggtaatac
    17341 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
    17401 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaatc
    17461 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
    17521 acgccatcac gctgaaacca attttcagag ccggtttacc cctgctttat ctaccgtaaa
    17581 caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg caatcatctg
    17641 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
    17701 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    17761 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
    17821 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaatacttc
    17881 ttccgcctca atatgacgcc ccttttccgc caaccagcgc gggctatttg gcaggaagac
    17941 caccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    18001 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    18061 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    18121 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agccccacac gagcggcgat
    18181 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    18241 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
    18301 accaattgct gcgccgagca tcatgctact aaccacccat tcctgcaaat gactggtcag
    18361 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    18421 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    18481 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    18541 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    18601 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    18661 aattaagttg aattattgag attattatta accacctaat tttacagcag ataaaattca
    18721 taaagttcat taattgataa ttaatatgga ttatttcata accatgatat ggattatgat
    18781 gatctacagg tataaaaaac cctgccatgc ggcagggtca taaaagtaag aagaatgaat
    18841 taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata atctgaagcg
    18901 ctggaggcaa ggaacactat cggccccatc aggtcactcg gcagtcccca acgaccagct
    18961 ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgtagttg ttgagtattg
    19021 ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg
    19081 ttcgccatca atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca
    19141 cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct
    19201 tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg
    19261 ttcatgacat cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat
    19321 ccggcgttat tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt
    19381 ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc
    19441 gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag
    19501 cccgcttgcg ccagccccaa cgccatcccc tggcccagcc cagtatcaca accagtgacg
    19561 accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc ttattcgttt
    19621 atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt
    19681 tttcaccaac catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg
    19741 accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg
    19801 gttgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt
    19861 ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct
    19921 ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa
    19981 tagtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccgggg
    20041 tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg
    20101 gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg
    20161 cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa
    20221 tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa
    20281 cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt
    20341 caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt
    20401 cattgtgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta
    20461 cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac
    20521 tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat
    20581 aaaacaatgt tttattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat
    20641 gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat
    20701 tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg
    20761 tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct
    20821 ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa
    20881 gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc
    20941 cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata
    21001 agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc
    21061 ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc
    21121 agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg
    21181 ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga
    21241 tttaaacggg ctaaaccttc tgcactggcg tcagtgcatg gctgttcatc ggtatcaaca
    21301 accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg
    21361 gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga
    21421 cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg
    21481 aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt
    21541 gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg
    21601 gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta
    21661 agcccagaac cgcaaacgtc attaatagtg attgcagaaa cgctattagg aagaccacct
    21721 ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga
    21781 attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc
    21841 aaactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt
    21901 gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg
    21961 caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc
    22021 acaaaagaaa tatgcctgag cagcagtcag agacataact ggcacgtaag gtttgcaacc
    22081 actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa cctggtttat
    22141 ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat
    22201 ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg
    22261 cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc
    22321 tctggtcgcc tggcctttaa catattggcc acataaagct ttatgccagt tcatcctctc
    22381 atcgaaaaca tcagcaggtg aagggataac gggcgcggta acacactact atggcaagaa
    22441 gattggtaat ctgattacca cgctgtactt ca
//
LOCUS       DAJVTY010000056        22300 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVTY010000056 DAJVTY010000000
VERSION     DAJVTY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22300
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.056"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            114..720
                     /locus_tag="OQQ66_001919"
                     /pseudo
     CDS             114..720
                     /locus_tag="OQQ66_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            823..1326
                     /locus_tag="OQQ66_001920"
                     /pseudo
     CDS             823..1326
                     /locus_tag="OQQ66_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1393..2850)
                     /gene="nuoN"
                     /locus_tag="OQQ66_001921"
     CDS             complement(1393..2850)
                     /gene="nuoN"
                     /locus_tag="OQQ66_001921"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1904869.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2857..4386)
                     /gene="nuoM"
                     /locus_tag="OQQ66_001922"
     CDS             complement(2857..4386)
                     /gene="nuoM"
                     /locus_tag="OQQ66_001922"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1904870.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4550..6391)
                     /gene="nuoL"
                     /locus_tag="OQQ66_001923"
     CDS             complement(4550..6391)
                     /gene="nuoL"
                     /locus_tag="OQQ66_001923"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1904871.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6388..6690)
                     /gene="nuoK"
                     /locus_tag="OQQ66_001924"
     CDS             complement(6388..6690)
                     /gene="nuoK"
                     /locus_tag="OQQ66_001924"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1904872.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6687..7241)
                     /gene="nuoJ"
                     /locus_tag="OQQ66_001925"
     CDS             complement(6687..7241)
                     /gene="nuoJ"
                     /locus_tag="OQQ66_001925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1904873.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7253..7795)
                     /gene="nuoI"
                     /locus_tag="OQQ66_001926"
     CDS             complement(7253..7795)
                     /gene="nuoI"
                     /locus_tag="OQQ66_001926"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1904874.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7810..8787)
                     /gene="nuoH"
                     /locus_tag="OQQ66_001927"
     CDS             complement(7810..8787)
                     /gene="nuoH"
                     /locus_tag="OQQ66_001927"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1904875.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALIVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8784..11510)
                     /gene="nuoG"
                     /locus_tag="OQQ66_001928"
     CDS             complement(8784..11510)
                     /gene="nuoG"
                     /locus_tag="OQQ66_001928"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1904876.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11563..12900)
                     /gene="nuoF"
                     /locus_tag="OQQ66_001929"
     CDS             complement(11563..12900)
                     /gene="nuoF"
                     /locus_tag="OQQ66_001929"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1904877.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12897..13397)
                     /gene="nuoE"
                     /locus_tag="OQQ66_001930"
     CDS             complement(12897..13397)
                     /gene="nuoE"
                     /locus_tag="OQQ66_001930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1904878.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13400..15202)
                     /gene="nuoC"
                     /locus_tag="OQQ66_001931"
     CDS             complement(13400..15202)
                     /gene="nuoC"
                     /locus_tag="OQQ66_001931"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1904879.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15296..15958)
                     /gene="nuoB"
                     /locus_tag="OQQ66_001932"
     CDS             complement(15296..15958)
                     /gene="nuoB"
                     /locus_tag="OQQ66_001932"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1904880.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15974..16417)
                     /gene="nuoA"
                     /locus_tag="OQQ66_001933"
     CDS             complement(15974..16417)
                     /gene="nuoA"
                     /locus_tag="OQQ66_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1904881.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17048..17986)
                     /gene="lrhA"
                     /locus_tag="OQQ66_001934"
     CDS             complement(17048..17986)
                     /gene="lrhA"
                     /locus_tag="OQQ66_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1904882.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18905..20122
                     /gene="alaA"
                     /locus_tag="OQQ66_001935"
     CDS             18905..20122
                     /gene="alaA"
                     /locus_tag="OQQ66_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1904883.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20206..20804
                     /gene="yfbR"
                     /locus_tag="OQQ66_001936"
                     /pseudo
     CDS             20206..20804
                     /gene="yfbR"
                     /locus_tag="OQQ66_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
     gene            complement(20863..>22300)
                     /locus_tag="OQQ66_001937"
     CDS             complement(20863..>22300)
                     /locus_tag="OQQ66_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1904884.1"
                     /translation="PSRLMMPLSFAGLISGMMTLVATPPNLVVNSELLREGYHGFSFF
                     SVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFRDLIREYRLTGRARRLA
                     IRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMS
                     AADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPESELIGKSVREIGFRTR
                     YGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGMLAKQGRDFVALNLPEEV
                     SEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLMGKFRCIDAESAYKSIH
                     WPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMMLGCLFVLSAVIGLFIS
                     NTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPVSSPVNTLVLGPGNYSF
                     SDFVKLGVPFTIIVMAVCVVMIPMLFPF"
BASE COUNT         5691 a         6142 c         5594 g         4873 t
ORIGIN      
        1 tttgtataga tctaacaacc gtaataaaac aaatactcat aactcattat aaataatgat
       61 aaaataacgt cttttattga taatttatat atgggaaaaa ttccttttgt tatattgttt
      121 ctgatttcac tccctctgat tatttttacc aaccctgggt cactgcatta ccaccaacgc
      181 cccgacacgt cagtcctgag caattagaaa aaacagttcg ttatcttaca caaactgtgc
      241 atccacgtaa tgccgacaat attgataatc tgaataggtc cgtagagtat ataaaagagt
      301 tctttgtcag tagcggtgcc agagttaccg cgcagggcgt ccccgattgg ctattacgac
      361 tctgcgcctg gtagccagga ttacctttac ccgcaatgtc ctggctttat cccgatcggg
      421 gggactttat tgcctggtcg gcagaataca ggatatcaac gccgttcgtc aggtaaaaac
      481 ggcattgtta tcatcccggg atttatctgt ttattctatg aatgccccat ggtttattcc
      541 cggtattgat ttctctgacc acctgaatta ttggcaacac gatattcccg ccgtaatgat
      601 tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata cagcagacag
      661 attgaattat cagaaaatgg ctcagatggt gttataacct tgttacacaa cagtaaataa
      721 taaaaatttc tcgaggcggg tccagaaaca gcgacatgta cattttattt tccctcgtct
      781 gttttaagat gcgaaacata aaattcaatg gaggaaaaat aaatgggaat gattggttat
      841 tttgcagaaa ttgactcaga aaagattaac caatctgctt gagtctactg aaaaaccttt
      901 gatggataat atacatgaca cgctttcagg attacgccga ctggatattg ataaacgctg
      961 ggatttttta cacttttggg ctgacaggca cccctgcttt tgatcccgcg aagaatgatc
     1021 cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga tggcatagat ggcttcctgg
     1081 gattgacctg gaatcaggaa ttagccgcga caatcgaccg actggaatca ctggatcgaa
     1141 gtaagctgcg taaacagttt tccattaagc gactatgaaa tggagattta tccgggagtg
     1201 accttttcag aagagttaga ggggcaactc tttgcctcga tcatgcttga tatggaaaag
     1261 ctgattagcg cgtatcgacg tatgctgcga caagggaatc atgcgctgac ggttattgtt
     1321 gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat gtcggcggtt ttctgactta
     1381 caaactaaca gattacatca gcggcattgc taaccgcaca atactaatca gcgattgtgg
     1441 ccatacaccc agcaccagta ccaacagtgc agaaatcagc accacgatac cgcccgcgct
     1501 gtactgccag tttgacggtg catcgcgacc cggttgttcc ggtgcgtgca ggtacaggct
     1561 caccgccacg cgcaggtagt agtagaggcc gattgccgaa ccgacaacca cggcacccac
     1621 cagccaccac aagtgtgcct ggacaccgac cgccagcacg tagaacttac cgataaagcc
     1681 cagcgtcatc gggataccgg ccagagacag catcatcacc gtcatcactg ccgcgaggat
     1741 cggacgatgc cagaacagac cgcggtaaga gaacagggaa tcagcatccg ggccacgata
     1801 cgggctggac atcaggctga ccacgccgaa cgcgccgagg ctgctgaaca gataaccggc
     1861 caggtaaacc cctaccgctt ccatcgacat ctcgccggtt tgcagcgcaa tcagcgccac
     1921 cagcagatag ccgaggtgag agatagatga gtaaccgagc agacgtttga tgttggtctg
     1981 gctcaacgcc atcaggttac cgaagatgat ggaggcaaag gcgataatcg ccagtactac
     2041 gcgaatcgct tcgctgtcac ccaccggtgc atagaggaac agacgcatca ccacaccgaa
     2101 gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc ggcgcaggcg cgccctggta
     2161 tacgtctggc gtccacaggt ggaacggcac cagagagagt ttgaagccga ggccaacaat
     2221 catcatgccg aaacctgcca gcaacagcgg ctcgttgagc ataccgtcgc cgaggttttt
     2281 acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac accagcgcca taccaaacag
     2341 caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg atactggctt ccagcgaacg
     2401 tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc aaagagatca gttcaatacc
     2461 gaggaacaga gacgccagat ggtttgcatt cgccagcagg atcccgccca gcgcggcaat
     2521 taacaccaac aggtagaact catccttgtt gtcgttatag ccttcaagcc acgggtaggc
     2581 gaaagtacag gtggcgaggc tcgccaacaa taccagcccg gtgtaaagca tggcgaaacc
     2641 atcaacgcgc atcagcggcg taacgtccat agcgcccgcc tggccaacaa accagagcga
     2701 aaccagcgcc gcgttaagcc caataaccga gagcgtagcg ttgaggaaat gattgcgtcg
     2761 ccacgcaatg gagagcatca caaccaccac cgttaagccg acgatcagca acggtagcag
     2821 tgcgatcagg ttttgtggag ttattgtcat ggcgatttac ggccttgtag tagtaacgga
     2881 attaacaaac cactgctgga tattgccaat cgcggagtgc gaggtatcca gaatcggctg
     2941 cggatagaag cccagcagta ccagcagcac caccagcaac aggatcataa acagctcacg
     3001 cagcgacatc cctggcagtt cctggctggc aatctggctt ttcgctttac cgaagtaagc
     3061 gcgatgcaac atcgccagcg aataaacaga tgcaaagacc agcccaaagg tagagataac
     3121 ggtaatcacc gggacaacct ggaagctgcc gaacagaatc ataaattcgc cgacgaagtt
     3181 accggtgcca ggcatcccaa gcgttgccac cgcaaagaac agcgacaatg ctggcagcca
     3241 tttcatcttg ctccacagac cgcccatcat gcgcatgtcg cgggtatgga tacgttcata
     3301 aagctgacca caaagaataa acagacccgc cgccgacaaa ccgtgcgcaa tcatctggat
     3361 taccgcgccc tggtaggcca actggctgcc ggtgtagata gcaatcagca cgaagcccat
     3421 gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg gtctgggcga aggccatcca
     3481 cgcaccgtag aagatgccga taacacccag ccacatagca attggcgcga actctgccga
     3541 cgcgttcggg aacagcggca gggagaaacg cagcaaaccg taagcggcag ttttcagcaa
     3601 gatccccgcg agatcaacgg aaccggcggt cggagcctgg gagtgcgcat ccggcagcca
     3661 gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg atgaagaagc ccagcattaa
     3721 caggtattcc acaccactgg acattggcgt attcagcagc tcttcatagt tgaaggtcca
     3781 gacgccggtc gcattgtagt gaacaaaaac cagcgccagg atggcaatca acatcaccag
     3841 gccactcgcc tgggtgtaaa tgaagaactt ggttgccgcc gtgatacgcg ttttaccgtc
     3901 agaggcttta tgcccccaca gtgcgatcag gaagtacatc ggcaccagca tcatttccca
     3961 gaagaagaag aacaggaaca tgtcgatggc aaggaacacg ccgataacgc cgcccaggat
     4021 ccacatcagg ttgaggtgga agaagccctg atatttttcg atctctttcc acgaacagag
     4081 taccgccagc acaccgagca gaccggtcag cacgaccatc agcagcgaca gaccgtcaat
     4141 ggcgagatga atagagatac caaaacgtgg gatccacggc atgtcgaatt cagactgcca
     4201 ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc tgcaaccaca gttgcagcga
     4261 cagcgccagc gtcaatccca tggtgatcag cgcgatccag cgtggcacct tgacgtcaaa
     4321 gtgttcggtc tgccagcaca ggaagccgcc aataaaggga attaatatta gccagggtag
     4381 taacatggcg atctttattc cttgtaaaag tcccgtcagg accggatttt caacgaattc
     4441 tcacgacaga aaattctctt cggactgggg gtagtgtcgg atgcgccgcg aaggcagctt
     4501 atccgaccta cgttcttata tgcccagggg caatcccaca atccttaact caacgcagta
     4561 ccatcaacag tgccagcacc acgaccgcac cgatgctcat ggatgccaca taccagcgca
     4621 gataaccgtt ctcgcttaac agcaggcctt tacctgcaaa gcgggaaaga accgccggaa
     4681 tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca ggcaataccc agaaacggct
     4741 tgacgaacac tttgtcgtac agccagtcaa agccccaggc gttgtaccac caggtgccca
     4801 gcagacggcc cggcgcactg ttggcaatgg aggtcaccag agtacgttta ccctgccaca
     4861 gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc agaggtaatt tccagggtca
     4921 acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac gctctgcagc ggcggtacaa
     4981 tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat cagcggcagg ctgtgagtta
     5041 cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg gaagacgatg aaaatcatac
     5101 ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc tgccaccatc agattgatat
     5161 gaccattcgc catcgcaccc gcgaggatct catccttact gaagaagccc gcagtgacca
     5221 gcggtagtgc cgacagtgct gcgccaccca ccaggaagca gaaataaacc agcggaatag
     5281 atttacgcag accgcccatc ttgaagatgt tctgttcgtg atggcaggcc agaatgacgg
     5341 aaccggatgc caggaacagc agcgctttaa agaacgcgtg agtcatcaag tggaaaatcg
     5401 ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta gccaatctgg ctcatggtag
     5461 agtaagcgag aacacgtttg atgtcggtct gtaccagcgc ggcaaaaccg gccagcagca
     5521 gcgtaaccgc cccgacaatg cccaccagat gcagaatttc cggcgtcatc aggaacaggc
     5581 cgtgggtacg ggcgatcagg tagacacccg cggttaccat ggttgcggcg tggatcagcg
     5641 cggagacagg cgtcgggccc gccatcgcgt cggcaagcca tgtctgcaac ggcaactgcg
     5701 cagatttacc gaccgcaccg cccagcagca tcagcgtcgc ccacatcagc atgttattgc
     5761 cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg gaagttcagg gtgcccagtt
     5821 cgttgtaaag aatgaacagc gcgaaagcga ggaacacgtc acccacacgg gtcacgacga
     5881 acgctttcat tgccgctgcg ccattcttcg gatcggtgta atagaacccg atcagcagat
     5941 aggagcacag gcccacgcct tcccagccga ggtacatcag cagcaggtta tcggcgagaa
     6001 ccagaactac catgctggcg atgaacaggt tggtgtaggc gaagaagcga gagtagccct
     6061 cttcaccgcg catgtaccag gaggcgtaca tgtgaataag gaaacccaca ccagtgacca
     6121 ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt aaaaccgatg ttaaagtcgc
     6181 ctaccgacat ccacgtccac agcggctggc tgtatgcctg ctcgccgtta gcgaagaaat
     6241 caacgccgat aaaggcggtt accagcgccg ccaggcccac agagccaacg ccgacgatcg
     6301 ccgaaacgtt ttcagaccag cgcccacggg agaacgccag caggacgaag ccaatcaatg
     6361 gcaaaataat ggttaaggca agcatgttca tccgcgcatc tcacttactg aatcgatgtt
     6421 caggttctgg cgacgacggt gaagttgcag cagcagcgca aggccgatac tcgcttctgc
     6481 cgccgcgagg ctgatggcga gaatgtacat cacctgaccg tcggtctgac cccagtagct
     6541 tccggcgacc acaaaggcca gcgcggaggc gttaatcatg atttccagac caatcagcat
     6601 aaacagcaga ttgcgacgga taaccagacc ggttaagcca agaacgaata agattgccga
     6661 gaggatcagt ccatgttgta aggggatcat gcgtgctcct ccgtttttct tttcgcgctg
     6721 tcgtctttac gattgctcag cacttcaccc gcacgctctt cacgaccgac gtggaaggcc
     6781 acaaccagac ccgcgagcag cagcatagaa gccagttcca ccgccagtac gtaaggcccg
     6841 aacagcgtaa tacccactgc tttagcactg attggcgtac cgtcgatacc ctgatcatta
     6901 acaccaagga tggcgtaaac aatcaccacc agcatgatgg ccgacaaaat tgccggacca
     6961 atccacacct gcggtttcag ccactggcgt tcctgttcga tttctgaacc gcccaggttg
     7021 agcatcatca ccacgaacac gaacagcacc ataatggcac ccgcgtagac gataatttcc
     7081 agcgcaccgg cgaagtaagc gcccagtgag aagaacaccc cggaaatcgc cagcagcgaa
     7141 ataatcaggt acagcagtgc gtgtaccgga ttggtatggg tgatcactcg caaggtcgca
     7201 agtatggcta tcaggccaca gatataaaaa gcgaactcca ttgcccctct ccttacggta
     7261 acaggctctt gacgtcgata ggcttggctt cgttctctgc ttcgccctta tctttgccgt
     7321 cgattgccat acctgccatc cggtagaagt tatattccgg gtatttgccc ggaccggaga
     7381 tcagcagatc ctctttttcg taaaccagat cctggcgctt gtattccccc atttcgaaat
     7441 ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc gcacagacca cagaaaatgc
     7501 agcgtgagaa gttgatgcgg aaaaattccg ggtaccagcg accgtctttg gtttctgctt
     7561 tttgcagcga gatacagccg accgggcagg ctaccgcgca gaggttacag gctacgcaac
     7621 gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc acgataacgg ggcggcagat
     7681 agaccggctc ttccgggtac attcgcgttt cgcgtttggc gaacgcgtgc aggccgatca
     7741 tccagatact acgaacctgg gtgccgaaac ctactaacaa ttcttttaag gtcatggtct
     7801 tattgcccct tattgcgcct gccagagaat gacagccgcc gttaccagca agttgatcag
     7861 cgtcagcggc aggcagattt tccagccgaa ggacattacc tggtcataac gcggacgcgg
     7921 taacgacgca cgaatcaaaa tgaacatcat cataaagaac gcggttttca acgcgaacca
     7981 gatgaatggc ggtaacaacg ggccttgcca gccaccgaag aacagcgtca caatcaatgc
     8041 agagatggtc acaatcccga tgtactcacc cacgaagaac agaccgaact tcataccgga
     8101 atattcaatg tggtaaccat ccgccagttc ctgctcggct tccggctggt caaacgggtg
     8161 acggtgacat accgccacgc ccgcgatggc aaaggtaata aaaccaaaga attgcgggat
     8221 aacgttccac acatgcgcct ggctgttgac gatgtcggtc atgttgaatg aaccggcctg
     8281 cgccaccacg cccatcaggg aaagcccgag gaacacttcg tagctcaggg tctgcgcaga
     8341 agcacgcatc gcacccagca acgagtattt gttgttactt gaccagcccg caaacagcac
     8401 cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa atcccgatgt tcaggtcggc
     8461 aaccacccaa cccggactga ctggcacaat cgcaaaggcc agcagcagcg aggtaaaggc
     8521 aatcatcggt gccagggtaa agatgacgcg atccgagaat ttcgggatcc agtcttcttt
     8581 aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa ccgccccagc caacacggtt
     8641 aggtccataa cggttctgga acagacccag caggcgacgt tcgccaaagc tcatgaatgc
     8701 cccacaggtg acaaccacca gcaggatcac caccgctttg aggatggtca gcaggatctc
     8761 aatcagttcc ggtgatatcc aactcattgt tgtgcctcct tgagatcctc aagatgcgcg
     8821 ccagccagca ccggagcaat gccggacata cccatcggca agcccacctg ccctgccgtc
     8881 agtccttcgg cgatttcaac cggcagcgtg accgtgttgc catcgtaact aaaggagacg
     8941 cgtgtacctg cgttcacacc caacttcgcg gcatccgctg ggttgagttt gatgtacggc
     9001 tgcggcatac ggctctggaa gaccggagca cgctgtgaca attcatcgct gccaaacagg
     9061 tggtaatacg gcgcaatacg ccatttcccg tcctgcggct ggaagcgtgc cggtacgctg
     9121 gtgaagtaat ccagaccatt ctcgctggtt tcaaacagac gcacgcccgg atcgccaaag
     9181 cgcagtttgc cgcccacttc gtcctggaat ttgttccacg cctgcgggga gttccagccc
     9241 ggtgcccagg caaacggcac ttgcgaacgg tgcgcggtcg gctggttgtt accttccatc
     9301 gagaaggtga acatggtgtc aatatcctgc ggctgacgcg gctcatgaac gctgatattg
     9361 gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac gggccagttt ctgcccacga
     9421 atacggaatg tcgcatccgg cgcagcatct ttgatacctg ccagttccgg gattttcgcc
     9481 acaacagcgt caatcacatg gtcgagctgc gtccagtcca cttcacggct cagcagggtg
     9541 ctgtgcagcg agtgcagcca gcgccagctt tccagcatga cagttttgct gtcgtaataa
     9601 gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt cattattaat taccgtaccg
     9661 tcgctttcag caaagctggc ggcggaaagt accagatggg cgttttccat gatcgctgta
     9721 cgctgatggt caaccaccat caccagcggt gctttagcca gcgcagcatt cacgcgggta
     9781 gcagaagcgt gacgatgcag atcgttttcc aacaccacca ccgcgtcggc gcgtccggtt
     9841 tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca taatgcccag ccccatgctg
     9901 ttgacggaac gggcaatcat ggtgataccg acgtcagcgc cgcgaccttt cagggctttc
     9961 gcgacgttag ccgccgcctg aatcacctct aagctaccgg cgttcgtccc ggagataatc
    10021 aacggtttct tcgcacctgc cagtgcctgc acgatgacgt cgattttgct ttgcagctca
    10081 ggttcgatac cgtcaaccgc tggtgcagag ttatccagcg catgggcgat ggcaaaacct
    10141 aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg ccgcgatatc atccaaacgg
    10201 gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac gttgaccgat gttaaggatt
    10261 gccgcaatct gccagtcagc cactttctgt gctgccgcca tttcgcgcgc tttacctttc
    10321 accgcctgac gcactgccag cgcgacgcgc gcgccggtct gggtaacgtc ttcgcccagc
    10381 accagtaccg catcgtaaga ttcgatttcg cgcagagccg gagtataaat gccgccttca
    10441 cgcagcactt tcagcgccag ttgcagacgt tcctgctcac cgtgagcgat accggtgtag
    10501 aagttttctt cgcccaccag ttcacgcagt gcaaagttgc tttccacgct ggcacgcgga
    10561 gaaccaatac cgatcacttt cttcgactga cgcagaatat ctgccgcgcc ctgcattgcc
    10621 tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct gtactggctg acgcggacga
    10681 tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt cgcagaggaa gtagtggttt
    10741 accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc cgtaacgttc accggggctg
    10801 atgttacagc cgatggaaca ttgctggcag atgctcggcg caaactgcat atcccattta
    10861 cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc cggtcgggca aatttcgacc
    10921 aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg ggcgaccgaa gtagacgttg
    10981 tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt aatctttgta gtaacgcaca
    11041 cagcggtagc aggcgatgca gcggttcatt tcgtgagata tgaatggccc caaatcctga
    11101 ttacggtggg tacgtttggt gaaacggtag cgacggaagc tgtgtccggt catcacggtc
    11161 atatcctgaa gatggcagtt accgccttct tcacataccg gacagtcgtg cgggtggttg
    11221 gtcattaacc actcgaccac actttcacgg aactgtttcg cttcttcgtc gtcaatggaa
    11281 ataaaggtgc catcggaagc cggtgtcata caggacatca ccaggcgacc acgcgtgtct
    11341 tccgcgtttt ggtattgctt caccgcacac tggcggcaag caccgacgct tcccagcgcc
    11401 ggatgccagc aaaagtaagg aatatcaagg cccagagaca gacaagcttc cagcaggttg
    11461 tccgctccgt tgacctcgta ttctttgccg tctacatgaa ttgtagccat tagcatgctt
    11521 ccagttttct cggtcaggga ccgagcgtta atcgaaattc ggttaccagc gctctttcag
    11581 caggttcggc tgaatcccat taatcaaatg ggtattgctg aacggctgtt tgattcccgc
    11641 ctcaaattct tcgcggaaat atttgatggc gctctgtaac ggctccactg caccaggtgc
    11701 gtgggcacag aaagttttac ccgggcctaa gaatcgacac agttgctcaa gtgtttcaat
    11761 atcgcccggc tggccttcgc cacgctccag cgcacgcaga attttcacgc tccacggcag
    11821 accgtcgcgg cacggcgtac accagccgca ggactcacgg gcgaaaaact cttccaggtt
    11881 acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc atcgccagcg ccgtacccag
    11941 acggctgccc gctttaccga tactttcgaa ttccatcggc agatcaaggt gcgcttcggt
    12001 caggaagtca gtccccgcgc cgcctggctg ccaggcttta aatttcagac catcacgcat
    12061 accaccggcg taatcttcga ggatctcgcg tgcggtggtg ccgaacggca gttcccacag
    12121 tcccggattt ttcacccgac cggagaagcc catcagcttg gtgccagcat ctttactttt
    12181 cgagatgttc tgataccact ccacgccgtt agcgaggatc gccggaacgt tacacagggt
    12241 ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg gaggttgccg ggaagggggg
    12301 tttcgagcgt gggttagcac gacgcccttc tagggagttg attaacgctg tttcttcccc
    12361 gcagatgtag cgccctgccc cggtatggac gaacagttcg aagtcgaaac ctgttcccat
    12421 aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca atggcgcggc gcagatttac
    12481 tgccgcttcg atatattcgc cacgcaggaa gatgtagcca cggtaagctt tcagcgcaaa
    12541 cgcggagatg agcatacctt ccaccagcag gtgcggcagt tgctccatca acaggcggtc
    12601 tttataggtg cccggctcca tttcatcggc gttacacagc aggtaacgga tgttcatgga
    12661 ttcgtctttc ggcatcaggc tccacttcag gccagtagaa aagcccgctc cgccgcgccc
    12721 tttcagacca gcgtctttta cctgattaac gatttcgtcc ggagacagcc cggtcagcgc
    12781 cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg tccagccaca ctggctgttt
    12841 gtcatcgcgc agacgccagg tcagcggatg cgtttcggga gtacggataa tgtttttcat
    12901 ttataccgct ccagcagttc agggatcgct tccggggtca gatgcgcgtg agtgtcctca
    12961 tcgatcatca tgtttggccc tttatcacag ttccccaggc agcaagttgg cagcagcgta
    13021 aagcggccat caaatgtcgt ttgccctggt ttgatgttca gctttttctc gagcgccgcc
    13081 tgaataccct gatacccgtt gatatgacag accacgctgt cgcaataacg gatcacatgg
    13141 cgaccaaccg gctggcggaa gatctgactg tagaacgttg ccacaccttc gacgtcgctt
    13201 gccggaatac ccagcacatc ggcgatcgcg tggatcgcac catccggcac ccagccacgc
    13261 tgcttctgaa cgattttcag cgcttcaatg gacgccgcac gcgggtcttc gtagtggtgc
    13321 atctcgtgct caatcgcttc acgctctgcc gcactcagct caaaagcctc ggtttgtggt
    13381 tgttgattct cgtgcataat tagcggtcca catctgacat aacaaaatcg atactgccca
    13441 gataaacaat caggtcagac accaggctgc cgcggatcgc cgccggaatt tgctgcaaat
    13501 gcgcataact cggcgtgcgg atacgagtgc gataactcat ggtgctaccg tcgctggtca
    13561 ggtagtaact gttgatcccc ttggtcgcct caatcatctg gaaagattca ttggcaggca
    13621 tcaccggccc ccacgacact tgcaggaagt gggtgatcag ggtttcgata tgttgcagcg
    13681 tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc tttgaacggg ccttccggca
    13741 tgttgttgag gcactgctca agaatgcgca gactctggcg cagctcttcc actttcagca
    13801 ttacgcgggt gtagcagtca gaaacaccac caccaaccgg gatttcaaag tcgaagtttt
    13861 catagccaga ataaggacgc gccttacgca cgtcgaagtc gatcccggta gcacgcaggc
    13921 ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc ataggcggca acgccctggg
    13981 aacgaccttt cagaatggta ttttgcagcg ccgctttctc gtaagacgcc agacgtttcg
    14041 gcatccagtc gaggaactca cgcagcaggc gatcccagcc gcgcggcagg tcgtgcgcta
    14101 cgccgccaat acggaaccac gccgggtgca tacggaaacc cgtgatcgct tccaccagat
    14161 cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg cgtcattgcg ccgacgtcct
    14221 gaataaaggt cgagatgtac agcaggtggc tgttgatacg gaacagttcg gagagcataa
    14281 cgcgaatgac gttaacgcga tccggcacgg tgatcccggc cagtttctct accgccagca
    14341 cgtaaggcat ttcgttaacg cagccgccga ggtattcaat acggtcggta tacggaatgt
    14401 agctgtgcca ggactggcgc tcgcccattt tctccgcacc acggtggtgg tagccgatgt
    14461 ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa aacgatacgg aaagccccgt
    14521 gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa gtcctcgttt tcggtgccgc
    14581 gcttcatccc ccactcttcc ggtttgaagg tcagggcttc catctccaga tcctgtttgg
    14641 ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc cgggtaatct ttacgcagcg
    14701 ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag gttcgggtga ccgtcgaaag
    14761 taatgccaaa cagatcccag gtttcacgct cataccagtt agcgttcggg aacagtttgg
    14821 tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac cttcagcatg atgtcgcggt
    14881 tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc cgcggcaggt aacccttcgc
    14941 ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa cagcatgacg taaggtttcg
    15001 gcagtttctt taagaaatcg ccaacttcca gtaattgttc acgcttgatc cacacaacgg
    15061 gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg cccaaaacgg ttgcgcagtt
    15121 cgccaattac cggatcatca agatgatcgc gggtctgcca ggcgggttct tgcgcggtta
    15181 agtcggtcat attgttcacc attgcaaatg gtccgtggtg actgtcggcg cagtaagctt
    15241 cgcgaaatag atttaggaat aagcgaagaa aaatcccttt gccgacaggc gcaaattaaa
    15301 tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc accgcgcttg cgttcgcgct
    15361 ctgattgcat attggcgcga taaacgccct gatcgccaac cacccaggag agcggacgac
    15421 gttctttgcc gatagattcc tgcaacagca tcagtgcctg catgtacgct tcaggacgcg
    15481 gcgggcagcc cgggatatac acatcaaccg ggatgaattt atcgacgccc tgcacaacgg
    15541 aataaatatc gtacatacca ccagagttgg cacaggcacc cattgagata acccattttg
    15601 gttccagcat ctggtcatac agacgctgaa taaccggtgc cattttggta aagcaggttc
    15661 ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg caatacttct gcgccaaaac
    15721 gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc aacgtaacag caggaaagac
    15781 cgaagttata cggccaaatt gagtttttac gaccccagtt aaccatgtca ttgagcttgc
    15841 ccataaacac gtttttgtta acttcttgct ccagagggtc ggttacgatc tcctgctttt
    15901 gcagggggta acggtcgttc tcaccgttgg gatctatgcg ggtgagcgta taatccatct
    15961 taatgcctcg cggttagcgt tgacgattag cgatactgtt cgtttccggg ttcatacgct
    16021 cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat acgcaccaga taaaccagac
    16081 ctgccagtaa cacaaaaata aaaattgcag cttccacaaa gcctacccag ccgctttcgc
    16141 ggatagaggt tgaccatgcg aacagataca gcgcttcaac gtcgaagata acgaagaaca
    16201 tggccaccag ataaaacttg gcggacaggc gtaagcgggc ggagccgacc gagtcgatac
    16261 cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg accgcctaaa aaccaaccac
    16321 ctaccagcat taggcaacac aggccaatgg caacgataag aaagatagcg aatgcccagt
    16381 gatgagcgat gacttcagtg gatgttgaca tactcattgc ttactcatca aaagtagcgc
    16441 cagattcact gctcttcacg gcagatggac gccacatcga ttcatgggga ggaataaaaa
    16501 aaaccttaca atcactgtag aaattctttt atacagctaa ttgatgtggt cttttactcc
    16561 tttctataac cttttgtcaa ctttaacaaa agtttcttca cattagttta cataatatca
    16621 acaccattag catttaatgc cctttcaccc cagatccttg acgactccag gataattaga
    16681 tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat tttgacagga
    16741 atttgtgtcg attcctcccc ccaaaagaga gtattttctt gatctgtgac acgcttttgt
    16801 cattccataa caaaaacgca gcaacaaatt tacgtatttt ttaacatcat tgtagcaggt
    16861 gatttttttc aggcgattat ttgtgcgttc gggacgtgaa tctctggtga ttgaaaaatg
    16921 aacagttttg tacgttctgc actatgcgag gaaggatttt tactaaaaaa aagccgctgg
    16981 ggtttaaaac acccccagcg gctcgttttt tacactattg tctcaggaat tatctatcgt
    17041 ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc ggagcagaca
    17101 ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc acctgggcca
    17161 gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt agcggcggta
    17221 agccatctac tccactcagc acacgcaggt ccgggctcat catctcaacc ggccttgccg
    17281 tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag gcgacataag
    17341 ccagtcgcca tggaatatct gctttattaa gcgttgccag caccatatcg cgaaacggac
    17401 ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg tattccgctg
    17461 cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct gaaggacgat
    17521 gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata taggcattac
    17581 gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga tttaacagga
    17641 aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg ccctgaagat
    17701 tactgaacat taatgatgag caggcctcat cattaaaacg caggattttc ctggcgtaac
    17761 caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga gcgaacagtt
    17821 ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac tgagtacgac
    17881 acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca aatgttctca
    17941 gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat tatcacttac
    18001 tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat tccatttgta
    18061 atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc atatgagttg
    18121 cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca cagaatatat
    18181 cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac atgcgcgtgt
    18241 ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct ggtcatacgc
    18301 gaacacgtac aaacagcaat ggtgaggcgg catcaagagc agggatccgt catttatgcg
    18361 agcattttac cccaaaaacc ttttatttat aagggccatt gcgaatcatc tgatgcaaag
    18421 ttatgttatg ttaggcaaag taatcttcta ttattaataa gcacatcaaa acctttttga
    18481 atattaaata ataattaatt agcatcatcc tcattcatta attccgctta acaatagttt
    18541 cacaccttgc cccattacga cacctcggaa atcatcacgc tgttaaactc tccgtaatgc
    18601 ggaaatatca tcatcgcgtt tcattagtga attcttaaat gaggcatttt cacacaatta
    18661 tcttacagat aaaaaaacca gacttacaat taagaatcag aacaattcac tatataacat
    18721 tgcatgtaaa gcatatacac ctcattattt tgtcatcatt aagttattaa cagcacaatc
    18781 gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag
    18841 tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag
    18901 attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg
    18961 tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa
    19021 catcggcaac ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat
    19081 acgcaacctg cctaccgctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa
    19141 agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta
    19201 catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg
    19261 ggacgaaatg ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc
    19321 cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga
    19381 tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa
    19441 cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca
    19501 gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctttacg acgacgctga
    19561 gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc
    19621 gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa
    19681 acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa
    19741 cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt
    19801 tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga
    19861 tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga
    19921 cgccaaacgc tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga
    19981 aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat
    20041 tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct
    20101 ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagcgtt
    20161 atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat
    20221 ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctaat gcgcaacgtg
    20281 ctgacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca ttcgctggca
    20341 gctatcaaaa atcgaaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg
    20401 atgtaccacg atgcctcaga agtgccaccg gcgatctccc tactccggtg aaatacttca
    20461 attcgcaaat cgctcaggag tacaaggcta ttgaaaaaat cgctcagcaa aaactggtcg
    20521 atatggttcc ggaagagctg cgggatatct ttgcgccgtt aattgacgag catgcatata
    20581 gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact gtgtgcatat ctgaaatgtc
    20641 tggaagaact cgcggccgga aataatgaat tcttgctggc aaaaacgcga ctggaagcga
    20701 cgcttgaagc gcgtcgcagc caggagatgg actacttcat ggaagtattt gttcccagct
    20761 tccatctttc gctcgatgag attagccagg attcaccgct gtaagcagcc ggagtctgcg
    20821 tcgcatcagg caataagcgc cggatgcgac atcaggctct tgtcaaaacg gaaacagcat
    20881 cgggatcatc accacacaaa ccgccatcac gataatggtg aacggtaccc ccaacttcac
    20941 aaagtcactg aagctgtaat ttcccggacc taaaaccagt gtgttaacag gtgaagaaac
    21001 cggtgtcata aaggcggcgg atgctgccat cgccacgacc atcgcgaatg gataaggcga
    21061 cacccccatc gttttggcag cagccagcgc aatcggagcc atcaacaccg ccgtcgcggt
    21121 attagagata aatagcccaa taaccgccga caagacaaac aaacacccca gcatcatatg
    21181 tggcccgtaa ccaccgccaa tgtccatcag ccctttcacc gccagcgcga cacctcccgt
    21241 tttctgtaat gccacagcaa acggcatcat cccaacgatc aaaataatgc tcggccagtg
    21301 aatggattta taggcgcttt cagcatctat acagcggaat ttccccatca gcaggcaggc
    21361 gatgatagcg gcaacaggat taggaatttc atctgtcagc attaacgcca ccattagcac
    21421 cagacagaaa atggcatggg gtgcctggct gtgcgcgggc gatgcttcac tcacctcttc
    21481 cggtaagttc agcgctacaa agtcgcggcc ctgtttggcc agcataccga tcagtttcca
    21541 gttacccaca accaggatga tatcgcccag cagcagaggc tcatccgcca gcgaaccttc
    21601 cagcgccacg ccattgcgct ttagccccac cacattcagt ccgtagcggg tacgaaaacc
    21661 aatttcgcgc accgatttac caatcagttc tgactcagga attaatgaaa tctctgccat
    21721 gcccacatca agggcctggt cagaaaaata ctcgccgcgc agtaccatcg gctccagcaa
    21781 ttgctcacta caaaattgcc ggagatcgac atcagccgca gacatatcaa taagcaaaac
    21841 gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg ataacgcgac gaaaacgccg
    21901 ccagcgttca acaccgatga cgttagcgcc ataacgctca cgtaatttga gatcatccag
    21961 ccgttgacca atcattggcg atccggggcg aatagccaga cgtcgcgcac gcccggtcag
    22021 tcgatattca cggataagat cgcgaaaggt tcgacgcgtc cagccttcgc gctgcggggt
    22081 ctgggtatcc cctttcagca tgaaacgcat cactaacata tacaaaatac ccagcaccag
    22141 cacgaccagg ccaacaggtg ttacgctaaa gaaactgaag ccgtgatagc cttcacgcag
    22201 caattcactg ttgactacca ggttcggcgg cgtcgccacc agcgtcatca tgccgctaat
    22261 cagcccggca aaacttaacg gcatcatcag acgtgacggc
//
LOCUS       DAJVTY010000057        22296 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVTY010000057 DAJVTY010000000
VERSION     DAJVTY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22296
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.057"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            353..1711
                     /locus_tag="OQQ66_001938"
     CDS             353..1711
                     /locus_tag="OQQ66_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCS1904885.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNHG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="OQQ66_001939"
                     /pseudo
     CDS             complement(1950..3335)
                     /locus_tag="OQQ66_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OQQ66_001940"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OQQ66_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1904886.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKAQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="OQQ66_001941"
     CDS             complement(3729..4109)
                     /locus_tag="OQQ66_001941"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1904887.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAATCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="OQQ66_001942"
     CDS             complement(4464..4898)
                     /locus_tag="OQQ66_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCS1904888.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="OQQ66_001943"
     CDS             complement(4886..5287)
                     /locus_tag="OQQ66_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904889.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="OQQ66_001944"
     CDS             complement(5547..6116)
                     /locus_tag="OQQ66_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCS1904890.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6789..6998)
                     /gene="hha"
                     /locus_tag="OQQ66_001945"
     CDS             complement(6789..6998)
                     /gene="hha"
                     /locus_tag="OQQ66_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1904891.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAELAFNSAV
                     DHRLAELTMNKLYDRVPASVWKYVT"
     gene            7697..7903
                     /locus_tag="OQQ66_001946"
     CDS             7697..7903
                     /locus_tag="OQQ66_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1904892.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            7989..8561
                     /locus_tag="OQQ66_001947"
     CDS             7989..8561
                     /locus_tag="OQQ66_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904893.1"
                     /translation="MQKLENRCDLLLTQHQKWMASVTRFIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPATPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGESRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELESWAVARKREKRELELMIDEYLLYAC"
     gene            8770..8877
                     /locus_tag="OQQ66_001948"
                     /pseudo
     CDS             8770..8877
                     /locus_tag="OQQ66_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            10054..11073
                     /locus_tag="OQQ66_001949"
     CDS             10054..11073
                     /locus_tag="OQQ66_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1904894.1"
                     /translation="MNNEQMLIKCKDPRKCRFYAEYLNHKYPNEFPQFAPDNPQNCIR
                     NVNSFLKEKYYKASNNSFGVFGSTAEEALSKEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            11183..12055
                     /locus_tag="OQQ66_001950"
     CDS             11183..12055
                     /locus_tag="OQQ66_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1904895.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12389..15508
                     /gene="ag43"
                     /locus_tag="OQQ66_001951"
     CDS             12389..15508
                     /gene="ag43"
                     /locus_tag="OQQ66_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCS1904896.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDDNTGGQ
                     QIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWIHA
                     GGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINK
                     NGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGWQ
                     VIKEGGTTAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSVV
                     AGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSG
                     AAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLAG
                     TTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTLT
                     QKTVNLNEGTLTLNDSTVTTDVIAQRGTTLKLTGSTVLNGAIDPTNVTLTSGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNMLQ
                     AGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGYN
                     SQATLNVTF"
     gene            15629..18145
                     /locus_tag="OQQ66_001952"
     CDS             15629..18145
                     /locus_tag="OQQ66_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904897.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLVLPVQIRTIQQSGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDISERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVISLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAFPPELPA"
     gene            18221..18676
                     /locus_tag="OQQ66_001953"
     CDS             18221..18676
                     /locus_tag="OQQ66_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904898.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHHVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(18797..19008)
                     /locus_tag="OQQ66_001954"
                     /pseudo
     CDS             complement(18797..19008)
                     /locus_tag="OQQ66_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19089..19910
                     /locus_tag="OQQ66_001955"
     CDS             19089..19910
                     /locus_tag="OQQ66_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1904899.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            19965..20450
                     /locus_tag="OQQ66_001956"
     CDS             19965..20450
                     /locus_tag="OQQ66_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1904900.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            20466..20942
                     /locus_tag="OQQ66_001957"
     CDS             20466..20942
                     /locus_tag="OQQ66_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1904901.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLGRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            21029..21250
                     /locus_tag="OQQ66_001958"
     CDS             21029..21250
                     /locus_tag="OQQ66_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1904902.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            21413..21781
                     /locus_tag="OQQ66_001959"
     CDS             21413..21781
                     /locus_tag="OQQ66_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1904903.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            21871..22245
                     /locus_tag="OQQ66_001960"
     CDS             21871..22245
                     /locus_tag="OQQ66_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1904904.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
BASE COUNT         5543 a         5859 c         5681 g         5213 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtta ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttatg gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggtgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcacg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac cctcaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgctacacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttca gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcctttt
     2461 ctgtccgggc tgtaagcgaa cctcactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc gatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 tgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagt
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttagg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtca ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaacatgg ccggacatgg gatcgtcttt cagcgccgtt tgtgctttcg cagccaggcc
     3661 gttgaagcca tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agtagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagcggcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggtgg cagcaacgag ctgctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgatactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata attttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtgtcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gcgagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggc
     6001 acatccggga gacgcagatc aacccgaagt gccgtcaggc ggggatgaac atccagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgttgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gtcctgctga ctactgtcag cgtcagtgtg
     6421 tgtatactgt gtagctggtg agataacggt tcatgtatcc cctgagctga caatgtaccg
     6481 tgtatattaa gcgtaaccga caggttctcc ttcataccat attaaatttt ccaggtattg
     6541 ccgctgcctt atttcgtccc atacaggaaa actcatctgg gtttctgaca aatacgcgaa
     6601 actcacagcc cctcaatatc aaccacacgt tgtaagccgg taatacctga aaatcagcct
     6661 attaccgccc acgcctccgg caccattccc ttttgatttc gaattctttg ttgccgttat
     6721 actgcttaat gttcccgatc ttccggtgca cagataacac cggagtcatc gtagcgcgga
     6781 ttcaagattc aggtgacata tttccagacg gaagccggca cgcgatcgta aagtttgttc
     6841 attgtcagtt ctgccagccg gtgatcgacc gcagagttaa aggccagttc cgcaggggta
     6901 agtttataac gtgtgtggga aatcactttt tccagcgtct cccgggatga acaacgacgg
     6961 aactgataca gccagtcttc tttggtttta acttccatta gcctctcatc actttatgct
     7021 gcaattaacg tgatactgcc agcataccgc acctgactgg cattctggta cccaccccaa
     7081 actcattact cccgcaccac cacttaaccg gtctctcaga ctgtcactca ccaggtcgga
     7141 tacgtttacc gctcagacgg atgcttaatg cactctgttc acgtgtgttg caggtacgtt
     7201 ttatatacgc aggttatacg ccagctatca gggatagccc tgtgagtaca acgtaatctg
     7261 ttgctactgc gttctcatac ccactgatca cactcacagt gtttatttca cagatatgaa
     7321 ctcatgtgtt tgatctctct tatatattta taataccaat cagacagtct tatcccatcg
     7381 gtgatacctg tctctgccca caaggcacga gctgccccac cgtaaccgcc ccgtaaataa
     7441 ctctgcaatt ataagaacga cggttcttcc cctcatcctc ccgaaaatca tcccttctct
     7501 gtcgtgcgta cgcgcgagaa acatcgtttc gcggtagcca ccgggtatcc cagcatcatg
     7561 ctccctgttt atctcccgac aggaatattc ccctgctcat tttttacgtt tctccccggt
     7621 tgttatgcca gtgaaggtgg tgcgtttttc atcaccaacg ccggttgaga ttaacaacat
     7681 ccggaggaac attttcatga ataccccagt ttcgctgatg gatgaccaga tggtcgacat
     7741 ggcgtttatc actcaactga ccggcttaac cgataagtgg ttttacaagc tcatcaagga
     7801 tggtgccttt ccggccccca tcaaactcgg ccgcagctcc cgatggctga aaagtgaagt
     7861 ggaagcctgg ttgcaggcgc gtattgcaca atcccgtccg taattcctga ccattacccg
     7921 ttcaaccgca gcgaatacac tatccggcct ccggaggcat tttgctgcgt gctaaaggag
     7981 agaatattat gcaaaaactt gaaaaccggt gtgatctgct gctcacccag catcagaaat
     8041 ggatggcaag tgtaacccgg tttattgtcg ctcacggaat gggctctccg catcttcatg
     8101 gttatcatcg cctgaccctg gcccactttt ttctgccgga aaaaggcacc atcatctcgg
     8161 tggcaccaca gggactctat caggtagtca acccggcaac gccaccgttc attcctgcca
     8221 ttcaggaagg gttaatgacc agcattcaga ctcatgaaat catgttactt acacatttta
     8281 acctgggtgg tgttttactc agtgaactac atcgtctggg agaaagccga ctggcaaaca
     8341 ggctgaacag tctgctccgg cgttttgacg atcgcgatct ctaccacacc ctgatatggc
     8401 tgtgctggta tgatctgatg tgcgcccact ccatgcagcc ctggactgaa gagttaaagc
     8461 ataaatcaca cgcagaactt gaaagctggg ctgtggcgcg caaacgggaa aagcgcgagc
     8521 ttgaactgat gattgacgag tacctgttat acgcctgttg attcataatc actcccgggc
     8581 ggtacgtagt cagtgatacc ggaatttccg gagagggtcg caggtatatt gtttctgtgc
     8641 aattcatggt tattcacgtt ggatgtagtg attgctgcat ccaatgctat cggaaagaga
     8701 ttaaccagca aatggttcct caaatcgtat tgatatttac ctggcgcatt gagcaccacc
     8761 atctgccccc gttcccacat caaacggctg gaccggaaaa cgatttgctt ttcatgctcc
     8821 gtggaaattc acgaaaaagt tattgggtca ttcatcgaaa aaaacatatt ctgctgactg
     8881 gagtcatcac ccagaaataa acgtcaactg aacaataacg attttttctt tgtgggattc
     8941 catacacttt tccatccccc tctgaaaatt agtcatcaac tcaccaacac atcttctgaa
     9001 atttgtatta aacttattgc ttaaatagat gatataatat ctcttccatt gcacggaatc
     9061 agcaaaaact gtcattctgg caatcttaac aagaacattt atcggttaat tttatcagta
     9121 aacaatggaa tatcatactg ataaaatgaa cattcagggt aagatgatga aattcacatg
     9181 aaaaatgctg aagattattt atttcatgca gataaacgat aaaaaacaag agagtatatt
     9241 tttcagtgta agagtcaact gttcatctcc actgacaatc acggtcagca tacaaacaaa
     9301 gtgagtcctc ctgcttagtc attgcgtatc agaaactaaa aaacaaaaga ttctgcaaac
     9361 aacgtgacat ttttatgaca tttcccgaat acatgtatat caataaacgg tagtcatcgt
     9421 tgaattaata agtcttacag agctatacgt agcttataaa accactcaac gaccatcatc
     9481 acctgactat ttagcgggag tatcgtctgt ataaatccat aaataataac acctgttatt
     9541 aaaataagaa tgctgtcagc cgtaaactat taacacaaca cctgtccact ctatctgttc
     9601 ttttaactca accggcgtcg aaaatatttc ctgattagtg catcatagtc ttcttttttc
     9661 tttctcaacc atcaaaccaa acacaacatt cacaaccaca caataaaaca aaaccaaaac
     9721 ataagattaa caatgccagt caacaggaaa acactgaata ttgaggtgcg ctgtaacctg
     9781 gacagcgcat acggcgcatg tgatcaacac agcccattct tttctgtcat cccccaaatg
     9841 cagaacaaac aatcataacc aaatgaatat aaaagaaaat atgatttttc accatcataa
     9901 aaacatcaat gtatctttat ataaaaacac tgataacatg acatagacat ttaaaaacat
     9961 aaacaaatca caacaaaaac atttgataat cacttagtga caaaataaaa ttgagacgat
    10021 tttgttataa gagaggcgac atggagtgag ataatgaata acgaacagat gcttatcaaa
    10081 tgcaaagatc cccgtaaatg tcggttttat gccgaatatc tgaatcacaa ataccccaat
    10141 gaattccctc agtttgcgcc tgacaaccca caaaactgta tcagaaatgt taattcgttt
    10201 ttaaaagaaa agtattataa agcttcaaac aattcttttg gtgtttttgg ttcgactgca
    10261 gaagaagctc tcagcaaaga aattaatgaa cttgaaatta ttgcagggca ggcaatactg
    10321 cctgaagaga gttttctctg gttgaaaact gacgaacttg cgactttctt tacctggact
    10381 actatttatc tgtcgaagga agaccataca aaattcccga aaataaacca taattcatca
    10441 ggaataaaaa acggaattga agtcaggaaa atacgaacag ttaccgatga aacgttaaat
    10501 acttacaaaa aaagtaacct tccggataat ccatcatccc ataacgaacg aataaaagta
    10561 atcatttcat attttgactc ctgccattct ttaattaata aaaatgcaaa aagcatatac
    10621 ctcgaggaac tcagacaggc gtggcttaaa aagaaaagcg cttgtcaaaa gtttaagtgg
    10681 ctccttcctg atgatgatga aatgtgtcag tggttctgga caagactgaa acaaaaacag
    10741 gatgaaaaac atccagtaca taacccagtg acccggtggt tcataccatc ttcaacgaaa
    10801 gaatattatc ttgcctctct tgtcgccttt gctttatgga aagaagcgcc tgataccatt
    10861 gaactgttca gaacacgaat aaatcatgcc tggcatcaga aaaaacaaaa ggacaaatat
    10921 cggagtgatg gtaaaaaggc cattaatata catataagaa gcaatgtgaa agaaatgctt
    10981 gatgagctca gaactgttta cggtctgaga acaggagagt tttttgaaat gctgattaca
    11041 gagaaatatc ttgaacacaa gcgaaggaag taattcatta cctgttacat tctaaataat
    11101 ctcagacaga tattatctcc gtgaatcccc cgccaccttt ccggtgcgcg gggttttgtc
    11161 ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa ttgaaaaacc
    11221 gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc gcaaactcac
    11281 cagtcacgaa cccgtgattg gcattatggg taaaagcggg gccggtaaat cttcactctg
    11341 taatgcactg tttcaggggg aggtcacacc ggtcagtgat gttcacgccg gcacccggga
    11401 agtgcagcgc ttccgtctga gtggtcacgg tcacagcatg gttatcactg acctgcccgg
    11461 ggtgggcgag agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg acattctgcc
    11521 tgaactggac ctggtgctgt ggctgattaa agccgatgac cgggccctgt ctgtggatga
    11581 gtatttctgg cgacacatcc tgcagtgcgg acatcagcag gtgctgtttg tggtgacgca
    11641 ggctgacaaa acggaaccct gccatgaatg ggatatggca ggtattcagc cttctcccgc
    11701 acaggaacag aacattcgtg agaaaacgga ggcggtattc cgtctgttcc ggcccgtaca
    11761 tccggttgtt gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg tcagtgcgct
    11821 aatgacagca cttcccgacc atgccgccag tcccctgatg acccgcctgc aggacgagct
    11881 gcgcacggaa tctgtccgcg ctcaggcccg tgaacagttt accggtgcgg tggaccggat
    11941 atttgacacg gcggagagcg tctgtgttgc ctctgttgcc cgtacggttc tgcgtgccgt
    12001 ccgtgacacg gtggtctctg ttgcccgcgc ggtatggaac tggatcttct tctgaacctg
    12061 ttgtggatga tgtcctccct gcctctgagt ctgctcacaa aagcgctgtt ttcgttactg
    12121 tctctcttgt ccgtgcaata gcctgataat agaataaaac gatcgatatc cattttatcg
    12181 atcgtttata tcgatcgtca tgctaataat aactcctgtt agcaacgtgc gcagatacac
    12241 acagacatga gattcaggga acaacagagc cacacgtcag aaacttccgt cagccggacc
    12301 tccggcactg taacccttta cctgccggta tccacatctg tggataccgg cttttttatt
    12361 caccctcact ctgattaagg aaatgctgat gaaacgacat ctgaatacct gctacaggct
    12421 ggtatggaat cacattacgg gcgctttcgt ggttgcctcc gaactggccc gcgcacgggg
    12481 taaacgtggc ggtgtggcgg ttgcactgtc tcttgccgca gtcacgtcac tcccggtgct
    12541 ggctgctgac atcgttgtgc acccgggtga aacagtgaat ggcggaacac tggtaaacca
    12601 tgacaaccag tttgtatccg gaacagctga tggcgtgact gtcagtaccg ggcttgagct
    12661 ggggccggac tctgatgaca acaccggcgg tcagcagata gcccggggtg gcactgccag
    12721 aaacaccacg gtcaccgcaa acggtctgca ggatgtcatg gcaggtggca gtgccacgga
    12781 tacggttatc agtgccggtg gaggacagaa tctgcgcggg caggcttacg gtactgtcct
    12841 taacggtggt gaacaatgga tacatgcagg cggcagtgca tccggaacgg tgattaacca
    12901 gtctggctac cagaccatta aacatggcgg gcaggcaacc ggaaccatcg tcaacaccgg
    12961 tgcagaaggt ggtccggagt ctgaaaatgt gtccagcggt cagatggttg gagggacggc
    13021 tgaatccacc accatcaaca aaaatggccg gcaggttatc tggtcttcgg ggatggcacg
    13081 ggacaccctc atttacgccg gcggtgacca gacggtacac ggcgaggcac ataacacccg
    13141 gctggaggga ggaaaccagt atgtgcacaa atacggactt gcgctgaaca cggtaattaa
    13201 cgaggggggc tggcaggtga ttaaggaagg aggaactacc gcgcatacca ccatcaacca
    13261 gaaaggaaag ctgcaggtga atgccggcgg taaagcgtct gatgtcaccc agaacacggg
    13321 aggtgcactg gttaccagca cagctgcaac cgtcaccggc acaaaccgcc tgggagcatt
    13381 ctccgttgtg gcgggtaagg ctgataatgt cgtactggaa aatggcggac gtctggatgt
    13441 gctgagcgga cacacagcca ctaatacccg tgtggatgat ggcggaacgc tggatgtccg
    13501 caacggtggt gccgccacca ccgtatccat ggggaatggc ggtgtactgc tggccgattc
    13561 cggggccgct gtcagtggta cccggagcga cggaacggca ttccgtatcg gaggcggtca
    13621 ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg gtgatacggc
    13681 cacggatacc acggtaaatg gcggactgtt cactgccaga gggggctcgc tggcgggcac
    13741 caccacgctg aataacggtg ccacgcttac tctttccggg aaaacggtga ataacgatac
    13801 cctgaccatc catgaaggtg atgcactcct gcagggaggc gctcttaccg gtaacggcag
    13861 ggtggaaaaa tcaggaagtg gcacactcac tgtcagcaac accacactca cccagaaaac
    13921 cgtcaacctg aatgaaggca cgctgacgct gaacgacagt accgtcacca cggatgtcat
    13981 tgctcagcgc ggtacaaccc tgaagctgac cggcagcaca gtgctgaacg gtgccattga
    14041 ccccacgaat gtcaccctga catccggtgc cacctggaat atccccgata acgccacggt
    14101 tcagtcggtg gtggatgacc tcagccatgc cggacagatt catttcacct ccgcccgcac
    14161 agggaagttc gttccgacaa ctctgcaggt gaaaaacctg aacgggcaga atggcaccat
    14221 cagcctgcgt gtacgcccgg atatggcgca gaacaatgct gacagactgg tcattgacgg
    14281 tggcagggca accggaaaaa ccatcctgaa tctggtgaac gccggcaaca gtggtacggg
    14341 gctggcgacc accggtaagg gtattcaggt ggttgaggcc attaacggtg ccaccacgga
    14401 ggaaggggct tttgtccagg ggaatatgct gcaggccggg gcctttaact acaccctcaa
    14461 ccgggacagt gatgagagct ggtatctgcg cagtgaagaa cgttatcgtg ctgaagtccc
    14521 cctgtacgcc tccatgctga cacaggcaat ggactatgac cggattctgg caggctcacg
    14581 cagccatcag accggtgtaa acggtgaaaa taacagcgtc cgtctcagca ttcagggcgg
    14641 ccatctcggt cacgataaca acggcggtat tgcccgtggg gccacgccgg aaagcagcgg
    14701 cagctatggc ttcgtccgtc tggagggtga cctgctcaga acagaggtcg ccggtatgtc
    14761 actgacgaca ggagtgtatg gtgctgcagg ccattcttcc gttgatgtta aggatgatga
    14821 cggctcccgc gccggcacgg tccgggatga tgccggcagc ctgggcggat acctgaatct
    14881 gacacataca tcctccggcc tgtgggctga cattgtggca cagggaaccc gccacagcat
    14941 gaaagcatca tcggacaata acgacttccg cgcccggggc cggggctggc tgggttcact
    15001 ggaaaccggt ctgcccttca gtatcactga caacctgatg ctggagccac gactgcagta
    15061 cacctggcag gggctctccc tggatgacgg taaggacaac gccggttatg tgaagttcgg
    15121 gcatggcagt gcacaacatg tgcgtgccgg tttccgcctg ggcagccaca acgatatgac
    15181 ctttggtgaa ggcacctcat cccgtgacac cctgcgcgac agtgcaaaac acagtgtgcg
    15241 tgaactgccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca gttcccgggg
    15301 ggacatgagc atgggtacag ccgcagccgg cagtaacatg acgttctcac cgtcacagaa
    15361 tggtacgtca ctggacctgc aggccggact ggaagcccgt gtccgggaaa acatcaccct
    15421 gggcgttcag gccggttatg cccacagcat caacggcagc agcgctgaag gttataacag
    15481 tcaggccacg ctgaatgtga ccttctgata attctgcatt gcctctctgt ggtcccggtc
    15541 atcatgaccg ggacccggac gggggaaaac gtttcagtgc cacattcact ggcattcaca
    15601 ataacatgat attcatcacg gagtgactat gttacagata gtcggcgcgc tgattctgct
    15661 gatcgcagga tttgccattc ttcgcctttt gttcagagca ttaaccagca cagcgtctgc
    15721 gctggcaggg ttcatattgc tgtgtctgtt cggtccggct ttactggctg gctatatcac
    15781 tgaacgcata acccggttat tccatattcg ctggctggca ggcgtatttc tgacgattgc
    15841 cggaatgatc atcagcttca tgtggggact tgatggtaaa catatcgcac tggaggctca
    15901 tacttttgac tctgtaaaat ttattctgac caccgctctc gccgctggtc tgctggttct
    15961 tcccgtgcag ataagaacca ttcagcagag cgggctcaca cctgaagata tcagcaagga
    16021 aattaacggg tattactgct gtttttatac tgcttttttc cttatggcgt gttctgcata
    16081 cgcaccattg atcgcattgc agttcgatat ttcaccctca ctgatgtggt ggggcgggtt
    16141 gttgtactgg ctggctgcat tagtgacgct gctatgggcg gccagccaga tccaggcgct
    16201 gaaaagactg accagtgcca tcagccagac actggaagaa caaccggtgc tcaacagtaa
    16261 atcgtggctg agcagtttgc aaaacgatta cagccttcct gaaacgctga cggagcgcat
    16321 ctggctgaca ctcatttcac aacggatttc ccggggagaa ctgagggaat ttgaactggc
    16381 agacggaaac tggctactgg acaatgcctg gtatgaaaga aacatggcag ggtttaacga
    16441 acagttgaaa gagaacctgt cattcacacc tgatgaactg aaaacgctct tccggaaccg
    16501 cctgaattta tcaccggaag cgaatgacga ttttctcgat cgttgcctgg acggcggtga
    16561 ctggtacccc ttttcagaag gccgccgttt tgtatcattc caccacgtgg atgagcttcg
    16621 tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc ataagccgga
    16681 tccggaatgg tactgctcct ctctttgtcg cgaaacagaa acactgtgtc aggacatttc
    16741 tgaacgttct tacaccggtt ttatttccga tgcaacggcg aatggtctga ttctcatgaa
    16801 actgccggaa acctggagta caaatgagaa aatgtttgct tccggagggc agggacatgg
    16861 gtttgccgct gaacggggaa accatattgt cgacagagtc cgtctgaaaa acgcacggat
    16921 cctcggtgat aataatgcca gaaatggagc agacagactg gtcagcggaa cagaaatcca
    16981 gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg gcattcgacg gacaaaacgg
    17041 acagtatcgt tacatgggaa atcatggtcc catgcaactg gaagtccccc gtgatcagta
    17101 tgccggcgct gtggaaacca tgaagaataa gatccgcgaa ggtaaagtac ccggtgtaac
    17161 cgatcccgca gaagcgtccc ggctgattcg tcggggacat ctgacttata cccaggcccg
    17221 taatatcacc cggttcggga ccatcgaatc ggtcacttat gatattgccg aggggtcggt
    17281 tatcagtctg gcggccggag ggatcagttt tgccctgacg gcatcggtct tctggctcag
    17341 caccggcgat cgcgatgctg ccctgcagac agctgctgtc caggcaggaa aaaccttcac
    17401 ccgcacactg gctgtctacg tcacaaccca gcaactccac cggctcagtg ttgttcaggg
    17461 tatgctgaag cacattgatt tttcgactgc cagcccgact gtccgactgg ctcttcagaa
    17521 ggggaccggt gcaggaaata tcagtgccct gaacaaagta atgaagggta cgctggtgac
    17581 atctctggca ctggtagctg tcacaaccgg ccctgacatg atcaaaatgt tgcggggacg
    17641 gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttccggtg tggcaggtgg
    17701 tgctgtcggg tcagtggcgg gcggaatact gttcagtcca ctgggaccat ttggtgcact
    17761 gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg tatcaggaaa
    17821 aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga ttcaggagca
    17881 ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa atctgaacga
    17941 gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg ccggtataca
    18001 acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca tcaggcaacg
    18061 ccccgtcatg gaatatgatg catcccatct cggcaatatg gttaaccgac tggaagaagc
    18121 attccccccg gagttaccgg cataacggct attctgcgcc ttttacgttt tatcacctga
    18181 tttgtcagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc tgttcaaaac
    18241 ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc gggcacagga
    18301 tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa atatttgtgc
    18361 ctaccagttc cggctggata atggcggaaa cgatgaagga tttggccccc tcaccatcac
    18421 tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg aaacggaagc
    18481 ctttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg agtgcgtgga
    18541 aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc atcatgtcag
    18601 tctgccgtta tcggttttcg atccccagga ctaccatcca ctgctgatta ccgtttccgg
    18661 aaaaaacgtt aactgatccc tgaagtcagt ttcatgcttc agcctctgtc acaacgcgtg
    18721 gcagaggctt tttatttatc aggagaaaac agatatgccg ggttacacag agtatgtcct
    18781 ggctgaagga tctttctcat acgggcaggc ggttgctgtc ataaccgctt accgcaatgt
    18841 gtttatccag gatgacccgg gaatgcattt tcgtcgggtt atccgcaacg cggaaggtca
    18901 gcgacgctga cgcagccgga actctgaagc tgatgccgga aaacagctta atgcgtggct
    18961 cgccagtgga gggcttctca gacaataaac ctcattgatt tcatccatca ggccgcgtct
    19021 tctccgggag acgcggcttt tttcatttat tacaccagta aatcttaacc accgataagg
    19081 agcaaagtat gcgattagcc agtcgttttg gttatgctgc aaaccagata cgtcgtgacc
    19141 gcccactgac acatgaagag ctgatgcacc atgtgccggg gattttcggg gaagataaac
    19201 acacgtcccg aagccagaac tatacgtaca tccccactat caccgtactg gaaagcctgc
    19261 agcgggaagg ttttcagccc tttttcgcct gccagacccg tgtgcgcgac ccgggccgcc
    19321 ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg agagataaac ggagaacatg
    19381 tccctgaaat tattctgctc aactctcatg acggtacctc cagctaccag atgctgccgg
    19441 gttacttcag gttcgtctgc cagaacggat gcgtctgtgg tcagtctctg ggggaagtgc
    19501 gtgttccgca ccggggaaat gtggtggaca gagtcattga aggggcttac gaggtggtgg
    19561 gcgtgtttga ccggattgag gaaaagcgtg atgccatgca gtcgctgatt ctgccgccac
    19621 cggcacgcca ggcgctggca caggcggcac tgacttaccg ttatggtgac gaacatcagc
    19681 ccgtcaccac cgccgacatt ctgacgccac gacgccggga ggattacggt aaggacctgt
    19741 ggagtgctta tcagaccatt caggagaata tgctgaaagg cggaatttca ggtcgcagcg
    19801 ctaaaggaaa acgtatccat acccgtgcca ttcacagcat tgacactgac attaagctca
    19861 accgcgcatt gtgggtgatg gcggaaaccc tgctggagag cctgcgctga ttcagccatt
    19921 accctgaaaa ccacgcattt atttgtcctt taaggagtca tttcatgaaa accatttcac
    19981 ataattccac cacatcttct gtttccgtaa ccgctgcatc agggaatgac cagcctcagt
    20041 tggttgccac cctcgtccct gatgaacagc gcatcagctt ctggccgcag cactttggcc
    20101 tcattccaca gtgggtgacc ctggagcccc gtgtcttcgg ctggatggac cgtctgtgtg
    20161 aagactactg cggtggtatc tggaatctgt acaccctgaa caacggcggg gcatttatgg
    20221 cacccgaacc ggatgacgat gatgacgaaa catgggtact gttcaatgcc atgaacggta
    20281 accgcgctga aatgagtccg gaagctgccg gcattgctgc ctgtctgatg acgtacagtc
    20341 atcatgcctg tcgtacggag tgttatgcca tgacggtcca ttattaccgg ctgcgggatt
    20401 acgccctgca gcatccggaa tgcagcgcca ttatgcgcat catcgactga atggagagaa
    20461 gtacaatgca acagctttcc tttctgcccg gagagatgac acccggtgag cgcagcctca
    20521 ttcaacgggc cctgaaaacc ctgggccgcc atcttcatga acccggcgtg gccttcacct
    20581 ccacgcatgc ggcacgggaa tggctgattc tgaacatggc gggactggag cgtgaagagt
    20641 tccgggtgct gtatctgaac aaccagaatc agctgattgc cggtgaaacc ctcttcaccg
    20701 gcaccatcaa ccgtacggaa gtccatcccc gggaagtgat taaacgcgcc ctgtaccaca
    20761 atgccgctgc cgtggtgctg gcgcacaatc acccgtccgg tgaagtcacc cccagcaagg
    20821 cagaccgcct tatcactgag cgtctggtac aggcactggc cctggttgat atccgggtac
    20881 cggaccatct gatagtcggt ggcagccagg ttttctcctt tgccgaacac ggtctgcttt
    20941 aacccgtcac cgtcacaatc accttcatat cacttcagtt tctctttctc agctgtttct
    21001 tactttcaca ttcaggagga ctattctcat gaaaatcatc acccgtggtg aagccatgcg
    21061 tattcaccgt cagcatcctg catcccgtct ttttccgttc tgtaccggta aataccgctg
    21121 gcacggtagt gctgaagcgt ataccggtcg tgaagtgcag gatattcccg gtgtgctggc
    21181 cgtgtttgca gaacgccgta aggacagttt tggtccgtat gtccggctga tgagcgtcac
    21241 cctgaactga tgttccgtca cccggaaaac gagtacgact ggctgagctc agacgccgct
    21301 gtggatgagg tcctgctcat caacgaatac acctttaccg aagccggtct gcgcgccggc
    21361 tgacccgtca tcgttaaact atcattcacc gattttctgt gaggatttta tcgtgtcaga
    21421 cacactttcc gggacaacgc atcccgatga caatgacgac catccctggt ggggactacc
    21481 ctgcacagtg acgccctgtt tcggcgcccg tctggtgcag gagggtaacc gcctgcatta
    21541 ccttgccgac cgcgccggta tcagaggcct gttcagcgat gcagatgcgt accacctgga
    21601 ccaggccttt ccgctactga tgaaacaact ggaactcatg ctcaccagcg gtgaactgaa
    21661 tccccgccat cagcataccg tcacgctgta tgcaaaaggt ctgacctgca aagctgacac
    21721 tctcggtagc ggtggttacg tttatctggc tgtttatccg acgcccgaaa cgaaaaagta
    21781 aaccaccgga ataaccttct gctccggcct ggtgctttca ccacgccact tttccatttt
    21841 tcatctctgc atatcaggaa aaccttcagt atgaaaacat tacccgatac acacgtacgg
    21901 gaggcatcga gctgcccgtc tcccgtcacc atctggcaga ctctgctctc ccgactactg
    21961 ggccagcatt acggccttac actgaatgac acaccgttcg ccgatgaacg tgtgattgag
    22021 cagcatattg aagctggcat ttcactgtgt gatgcggtga attttctcgt ggaaaaatac
    22081 gcgctggtgc gtaccgacca gccgggattc agcacttgtc cccgctctca gttaataaac
    22141 agtattgata tcctccgggc tcgccgggcg accggcctga tgacccgcga caattacaga
    22201 acggtaaata acattaccct gggtaagcat ccggagaaac gatgaaactt tccctgatgc
    22261 tggaagccga cagaattaat gtgcaggcac tgaaca
//
LOCUS       DAJVTY010000058        22047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVTY010000058 DAJVTY010000000
VERSION     DAJVTY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22047
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.058"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1103
                     /locus_tag="OQQ66_001961"
     CDS             <1..1103
                     /locus_tag="OQQ66_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1904905.1"
                     /translation="PRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKG
                     KAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGD
                     IAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVD
                     IYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLA
                     IVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRD
                     RILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSERL
                     YRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1283..2077
                     /gene="potC"
                     /locus_tag="OQQ66_001962"
     CDS             1283..2077
                     /gene="potC"
                     /locus_tag="OQQ66_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1904906.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2074..3120
                     /gene="potD"
                     /locus_tag="OQQ66_001963"
     CDS             2074..3120
                     /gene="potD"
                     /locus_tag="OQQ66_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1904907.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3276..4097)
                     /gene="cobB"
                     /locus_tag="OQQ66_001964"
     CDS             complement(3276..4097)
                     /gene="cobB"
                     /locus_tag="OQQ66_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1904908.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4113..5024)
                     /gene="nagK"
                     /locus_tag="OQQ66_001965"
     CDS             complement(4113..5024)
                     /gene="nagK"
                     /locus_tag="OQQ66_001965"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1904909.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5053..6297)
                     /gene="lolE"
                     /locus_tag="OQQ66_001966"
     CDS             complement(5053..6297)
                     /gene="lolE"
                     /locus_tag="OQQ66_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1904910.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6297..6998)
                     /gene="lolD"
                     /locus_tag="OQQ66_001967"
     CDS             complement(6297..6998)
                     /gene="lolD"
                     /locus_tag="OQQ66_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1904911.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(6991..8190)
                     /gene="lolC"
                     /locus_tag="OQQ66_001968"
     CDS             complement(6991..8190)
                     /gene="lolC"
                     /locus_tag="OQQ66_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1904912.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSINPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8452..9525
                     /locus_tag="OQQ66_001969"
     CDS             8452..9525
                     /locus_tag="OQQ66_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1904913.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9653..13099
                     /gene="mfd"
                     /locus_tag="OQQ66_001970"
     CDS             9653..13099
                     /gene="mfd"
                     /locus_tag="OQQ66_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1904914.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13243..14205
                     /gene="ldtC"
                     /locus_tag="OQQ66_001971"
     CDS             13243..14205
                     /gene="ldtC"
                     /locus_tag="OQQ66_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1904915.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14288..14545)
                     /gene="bhsA"
                     /locus_tag="OQQ66_001972"
     CDS             complement(14288..14545)
                     /gene="bhsA"
                     /locus_tag="OQQ66_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS1904916.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14786..15418
                     /gene="comR"
                     /locus_tag="OQQ66_001973"
     CDS             14786..15418
                     /gene="comR"
                     /locus_tag="OQQ66_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1904917.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15480..16019)
                     /locus_tag="OQQ66_001974"
     CDS             complement(15480..16019)
                     /locus_tag="OQQ66_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1904918.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16246..17550)
                     /gene="ndh"
                     /locus_tag="OQQ66_001975"
     CDS             complement(16246..17550)
                     /gene="ndh"
                     /locus_tag="OQQ66_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1904919.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(17950..18492)
                     /locus_tag="OQQ66_001976"
     CDS             complement(17950..18492)
                     /locus_tag="OQQ66_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1904920.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18515..19540)
                     /gene="nagZ"
                     /locus_tag="OQQ66_001977"
     CDS             complement(18515..19540)
                     /gene="nagZ"
                     /locus_tag="OQQ66_001977"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1904921.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19551..20375)
                     /gene="thiK"
                     /locus_tag="OQQ66_001978"
     CDS             complement(19551..20375)
                     /gene="thiK"
                     /locus_tag="OQQ66_001978"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1904922.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20359..20997)
                     /gene="lpoB"
                     /locus_tag="OQQ66_001979"
     CDS             complement(20359..20997)
                     /gene="lpoB"
                     /locus_tag="OQQ66_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1904923.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21011..21388)
                     /locus_tag="OQQ66_001980"
     CDS             complement(21011..21388)
                     /locus_tag="OQQ66_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1904924.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21391..21750)
                     /gene="hinT"
                     /locus_tag="OQQ66_001981"
     CDS             complement(21391..21750)
                     /gene="hinT"
                     /locus_tag="OQQ66_001981"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1904925.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5471 a         5800 c         5479 g         5297 t
ORIGIN      
        1 caccgaggcc acgaaaaaac agcactgacg tagccggtct ttacgaaaag tttgatcgca
       61 gaactggcag agtttactac cagtataaaa atcctgtgac tggaaaattt cacggactcg
      121 gaacagacaa aggtaaggca gaaaaaatcg cttccacagc caatcagcga atagctgcag
      181 cagaagctga atatttcatg cgcaaaattg atgaaagtcc gtcagcaaca aaacgtcggg
      241 gtatcagatt aaaggcatgg gttgatcgat atctgaaaat acaggacacg cgactgaaaa
      301 atggagatat tgcagctaca actcacaaag aaaaaactcg aatggctgca tacctggttt
      361 cccgtctggg aaaccaccca ttgaaagaac tggaagtaag agactttgca ttaatactgg
      421 atgagtggct ggataaagac atggtcagca cagcgagagt aaatcgtgga ttatgggttg
      481 atatttataa agaagcacag catgcagggg aagttcctcc tggatggaat cctccggagg
      541 ctacccgtaa accgatccct aaagtaacca gagccaggct caccatggaa gactggcaaa
      601 aaatttacaa tgcaacgcct gaaaaacact ttatccgtaa cgcaatgctt cttgcgattg
      661 ttactggtca gcgccgtgat gacatttgcc acatgcgttt ttcagatgtg tggaacgaac
      721 acttgcatat cacccaggga aaaaccggaa tgcgtctggc gttaccgctt acactacgct
      781 gtgatgccat tgggataacg ttaaaagaag ttattgatgg gtgccgagac agaatattaa
      841 gtccatatct aatccatagt cggcaccaga aacaaccgaa gccgatgagt aaagacaacc
      901 tgagcgacta ctttgccaaa gcacgggatc tggctgggat aattccacca gcaggaaaaa
      961 ctccgccaac atttcatgaa caacgctctc tatcagaacg gctgtaccgt gcacagggta
     1021 tcgatacaaa aacattacta ggacataaag tccaggcaac caccgatcgc tataacgata
     1081 ctcgaggtca ggaatgggtt taagctggtt atttgacaaa caccatatgg aatatccgtc
     1141 accatctcag gaaaaaagtg ttgtaaaatg cgtggcctag ttttggggaa acgttttgga
     1201 gagattttgg agaaaggaaa aaattgaata aattcaaaat cctgaaagtg ctccacctta
     1261 tgaataagaa ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt ttatgaccgc
     1321 tatctacgcg tacctgtata tcccaatcat tattttgatt gtgaactcct ttaacagctc
     1381 gcgctttggc atcaactggc agggttttac caccaaatgg tatagcctgc tgatgaacaa
     1441 cgacagcctg ttacaggcag cgcagcattc actaacaatg gcggtgtttt cggcgacgtt
     1501 tgctacgctt atcggttcac tgacggcagt tgcgctgtac cgttatcgct ttcgcggtaa
     1561 gccgttcgtt agcggaatgc tgtttgtggt aatgatatca ccagatatcg tgatggcaat
     1621 ttcgctgctg gtgctgttta tgctgctggg tattcagctt ggcttctggt cgctgctgtt
     1681 ctcgcatatc accttctgcc tgccatttgt ggtggtgacg gtgtattcgc gcctgaaagg
     1741 ttttgacgtg cggatgctgg aagcggcgaa agatctcggt gccagcgaat ttacaattct
     1801 gcgaaaaatc atactgccac tggcaatgcc agcggtggca gcgggctggg tgttaagctt
     1861 taccctgtcg atggacgatg tggtagtttc ttcgttcgtc accggaccaa gttatgaaat
     1921 tctgccgtta aaaatttatt cgatggtcaa agtcggcgta tcgccggaag ttaacgcgct
     1981 ggcaaccata ttactggtgc tgtcgctggt gatggttatt gccagtcagc ttattgctcg
     2041 tgataaaacg aaaggtaaca caggggacgt taaatgaaaa aatggtcacg ccacctgctc
     2101 gcggcgggtg ctctggcact gggcatgagc gccgctcacg ccgatgacaa caacacgctg
     2161 tatttctaca actggaccga gtacgtgccg ccaggactgc ttgaacagtt caccaaagaa
     2221 acctgtatta aggttatcta ttcgacttac gagtcgaacg aaaccatgta cgccaagctg
     2281 aaaacgtaca aagacggtgc gtatgacctg gtggttcctt caacctatta cgtcgataaa
     2341 atgcgtaaag aagggatgat ccagaagatc gacaagtcga agttaacgaa cttcagcaat
     2401 ctcgatccag acatgctcaa caagcctttt gacccgaata acgactactc cattccgtat
     2461 atctgggggg cgacggcgat tggtgttaac ggtgatgcgg tggatccgaa atctgtcacc
     2521 agctgggccg atctgtggaa gccagagtac aaaggcagcc tgctgttgac cgacgatgcc
     2581 cgtgaagtgt tccagatggc gctgcgtaag ctgggctatt ccggtaacac caccgatccg
     2641 aaagagattg aagctgcata taacgagctg aaaaaactga tgccaaacgt cgcagcgttt
     2701 aactccgata acccggctaa cccgtacatg gaaggcgaag ttaacctcgg catgatctgg
     2761 aacggttctg ctttcgttgc acgccaggcg ggtacgccta ttgacgtggt gtggccgaaa
     2821 gaaggcggca tattctggat ggacagcctg gcgatcccgg caaatgccaa aaacaaagaa
     2881 ggcgcgctga aattgatcaa cttcctgctg cgcccggatg tggcaaaaca ggttgctgaa
     2941 actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc tgttaagtcc agaagtggcg
     3001 aacgataaaa cgctctaccc ggatgctgaa accattaaaa atggcgaatg gcagaatgac
     3061 gttggcgcag ccagcagcat ttatgaagag tattatcaga agctgaaagc aggacgttaa
     3121 ttcagcaagc gggcggtggt attaccgccc gttttttatt tagcaaggat gtggctaatg
     3181 agtcaggaca gtagagtttt atccgactct ttcgtcggta ttgtggtccg ctcgtaggcc
     3241 tgataagcgt agcgcatcag gcaatgcttc ctgctttata atcccttcag caacttttca
     3301 acaaactcag gcaccacctg gcttgccggg ccgtaatatt tctcggcaaa ttcattaccg
     3361 acctgacttg gttcgagatt cagttccacg gtgtgcgcgc catgcagttt cgcttcgtga
     3421 acaaacccag ccgccggata aacatgcccg gaagtaccaa tggcaatgaa aatatcagcc
     3481 atcgacaacg ccatataaat ttcatccatg ccgagtggca tttcgccaaa ccaaacgacg
     3541 tgcgggcgca agggtgccgg gaactggcaa caatggcatt tatcttctgg cgtaacgtct
     3601 ccggtccagt cgagaacctg accactttgc gaacaacgaa ctttcagcaa ttccccatgc
     3661 atatgaatga cattggtatt acctgcgcgt tcatgcaggt tgtctatatt ctgcgttacc
     3721 agcaaaaagc gatcgccgag agcgtcttgc agtttagcca gcgcaagatg cgcggcgtta
     3781 ggctgaattt ttggctgctg cagctgtcga cgacgggcgt tataaaacgc ttgcaccagt
     3841 tcaggatcgc gatcgaaacc ttccggcgtt gccacatctt caacccgatg ttcttcccac
     3901 aggccatctg cggcgcgaaa ggtacgaata cctgattccg cagaaattcc tgcccctgtc
     3961 agtacgagta ctcttggttt ttccattgct tccggcacca ctttatctct gaaaaaaata
     4021 cgctgacgca aacgctcgcg caggcggcgt ttatttttac gaaaacgact taaccgatga
     4081 ccccgacgcg acagcatagc aacctctgtt gtttaatcgg ttagatgtag gaaggccgca
     4141 ccacgcattc ctcccgcatc accgtggcgc gcgcgttcaa tgcgcggaac acgagctaca
     4201 ggtaagagat gacgaggcag cctgtccgcc agttgcgttg tgattgccgg gaaattcgat
     4261 aagccaccac caatgacgac caggtcaggg tcaacaatgg tcaggatatt tcccagacaa
     4321 accgctaata aatccagata acgctcaacg tgcgcccttg cctgctcatc gccttgatta
     4381 taaagcgcaa taatttcggg agcctgcaac tgttgatgat aatagtgttg atacagccac
     4441 gcaaaaccgc gaccagacag ataattctca atgcagccat gctgaccaca gccgcagcgg
     4501 cgtaacggga aatccaaccc catcatggtt aacgcatcaa ccggcagacg catatggcca
     4561 aactcgccgg taatgtagct tttaccggta atcggtttgc cgttgaaaat cagcccaccg
     4621 ccaacgccgg taccaagaat caaccccatc accagtggat attgcgtaaa ttcatcatcc
     4681 caggcttctg aaagggcaaa acagttggca tcgttatcaa ggcgtacatc gcgatcaaga
     4741 cgtgcgctca ggtcggcacg cagcggttta ccgctggcag cggggacatt ggcggcatac
     4801 agcgtaccat cttctgtttc cggcattccc ggtataccaa tgccgacaga acctctacag
     4861 ccaaaacgtt gatcagcttc ggctaccagc tcgcacactg catctaaaaa tgcgtcatag
     4921 ctgtcacgcg gtgtcggcac ccgcttttcc cactgcaact gccgaccgct atcaaacacg
     4981 ccaagcgcaa tttttgttcc accaatatca aacccgtaat acattgccgc tccttgtttt
     5041 aatgtactgc ctttactggc cgctaaggac tcgcgcaggg tcaatattac tggcgcgccg
     5101 cgccggatac caacttgcca aaagactcag caacaatgct gtgaccagta cgtagaagac
     5161 gtccagccag tgcaattccg atggcaggaa gtcaataaaa tagatatcgc tggagaggaa
     5221 ctgatgaccg atcagctttt caatccgctc aataatcggg gtaagttgca gtgaaacaac
     5281 tacgccgata atcagcccac acaggctgcc gaacagccct gccagcaatc cataccagac
     5341 aaagatggcg cgaattaaac catctttcgc ccccagcgtt cttaatactg cgatatcgcc
     5401 actcttatct ttcaccgcca tcactaaggt ggagacgatg ttgaaacagg ccacgccaat
     5461 caccagtacc atcgccagat acataatggc gcggatcatc tggatatcgc gatacatata
     5521 gccgtaagta ccaatccagc ttttaatata aacatagctg ttggtcattt cacccgcatc
     5581 gcgtaccagc ttattggcgt tgaaaacatc cgtcatttta agggcaatac ctgacacgct
     5641 ggaacccata tcaagatatt gttgggcatc cgccagcggg atcatggcaa aactgtgatc
     5701 gagttggcca ctcaactgca aaatacccgc aacgtgcaaa cgcacacgtt ttggctgcat
     5761 cagtttatgc tcaggattcg agttggggat catgatcgac acccaatcgc cctgcttcac
     5821 tttcagcgca tccgccacgc ctttgccgat gataatttgc tgttcgcccg ctttaaaatt
     5881 gcgccacgca tccccctgaa caaacgaggg taatgcgctc agacgctgtt cctgttgtgg
     5941 gttaacgccc ttcacctgga ttgcgcgcag attcgctcca ctttccacca gcccggtgaa
     6001 attgatatac ggcgcagcgg cggcaatacc cggcactttc tgcactttat ccagtgcttc
     6061 ctgccagtta gtccatggct gatttaccgc ttcgatttca ccatgcggca ccaccgccag
     6121 aatgcggtta ttcagttcgc gttcaaagcc gttcatcgcg cttaagccga cgatcaatac
     6181 cgccacaccg agggcaatgc caatggtaga aatgacggag atcagcgaca ccatgccgcc
     6241 gcgccgccgt ccgcgactaa aacgcaggcc aattaataac gataaaggca tcgccattac
     6301 tccgccccca tcaggctcag ttccgccgtc agacgtccat cacgcatttc taactggcgg
     6361 ctcatacgtt tcgccagttg caggtcgtga gtaaccacca ggaaggcggt gccctgcaag
     6421 cgattcaatt ccccaagcaa ctggaagatg ctgtcggcgt tacgcgcatc gaggttaccg
     6481 gtaggctcat ccgccagtac cagacgcgga ttattgacca gcgcacgggc aatcgctaca
     6541 cgctggcgtt cgccgccaga aagttcagat gggcggtgat tcgcacgatg ctccagcccc
     6601 accgctttta acatctccag tgcacggctg ttgatttcag cgggcttttt cttgccaatc
     6661 agcagcggca tagccacgtt ttccagggcg gtaaaatccg gcagcaggtg gtgaaactga
     6721 taaataaagc ccagtttctg gttgcgcagt tctgcttttg ctgccgacga cagtttgctc
     6781 atcggttgac cgttaaagat cacatcgccg gaggttggcg tatccagtcc gcccagcagg
     6841 tgcagcaagg tgcttttacc ggaaccagag ctaccgacga tcgccatcat ttcaccttcg
     6901 ccgacgctga aactgacatt gtgcagtaca tcggtttgca cactgccttc ctgatagcgt
     6961 ttgcacaggt tgtcgcattg caacaggatc ttattcataa cgtaaagcct cagcgggttg
     7021 tgtggcggca gcgcgccatg aagggtaaag cgtagacagc agcgcgatag ccatcgccac
     7081 cagcgcaata acaatgacct gtaaaggttc gatagccatc ggcagcgccg cgccatcaag
     7141 caggacgccg attattggca tcagattatt taactggctg gcaagcagtg cgccaagcgc
     7201 cgctccgagg atcgcaccga taatcccggc gctggcccct tgcaccataa agatcatcat
     7261 gatttgtcgc ggagttaagc cttgcgtttg caggatcgct acttcgccct gcttctccat
     7321 caccatcagc cccagcgagg taataatgtt aaacgccgca acggcgacaa tcaggctcag
     7381 cagtaaaccc atcatatttt tttccatgcg tacggcctgg aacaactcgc ctttacgatc
     7441 acgccagtcc tgccatttgc tgtcttcagg cagtttttgc tgacttaatg agtcgacttt
     7501 cagcggctca tccagccaca aacgccagcc ggtaatattg cctgccggat aacgcatcag
     7561 acgcgaggca tcctcaatat tcaccagcat ttcattgcca tcgacttcac tgttggcggc
     7621 gaaagtgcca atcacattga acaggcgctg acttggaata cgccccatcg gcgtgaactg
     7681 gctggcagat ggtaccatca tgcggatttg atcaccgcga ttaacgccta gctgtgaggc
     7741 aagttgttcg ccgaggatga cattatattt ccccggctcg aggtcagttt gtttcacatt
     7801 gaccagatac ggcgttagtg gatctttttg cgccggatcg atacccagca tcaccccgac
     7861 cgccacgctg cgcgcgcttt gcagtaccac atcaccggta gtaataggtg cgacgcgatt
     7921 aacgccgtcc agtttgactg ccgtttccgg gagttgctgc gggttaatag agccatgctc
     7981 agaagagaga attgcctgtg gcatcaggcc aaggatgttg ttttgcagct cgcgctcaaa
     8041 gccgttcatc actgacaata ctgtgaccag cgccatcacc ccgagggtaa tgccgatggt
     8101 agaaagccag gagacgaaac gaccgaagcg atccgctgca cgcccacgca tgtaacgcag
     8161 gccaataaat agagcgacag gttggtacat gaaatccgtc tggttgctgt agcaaagaca
     8221 cgagtatata agcgaaagca agatgggtaa acgtctgaac tgtaagtgat cgtcagggct
     8281 aatctgaaaa gcccccgtaa atcaatcggc taaattgcca tgttttatca tcaatcttgc
     8341 cgcctgatct taatctgaaa aatcatccaa tacagacaga tacccattac acctcgggtc
     8401 ataacccgca ggatcaaccg catgtaaatt aaaaaagagc gtgttgtcct gatgaaacaa
     8461 aaagagctat ggattaacca gatcaaaggg ttatgtattt gtccggtggt gatttatcac
     8521 tcggtcatta ccttttatcc gcatatgacc acttttccgc atccgttatc ggaagtcctg
     8581 agcaaatgct ggatctattt caatctttac cttgcaccct ttcgtatgcc ggtttttttc
     8641 tttatatccg gctatttgat tcgccgctat atcgacagcg taccgtgggg aaattgtctc
     8701 gataaacgga tctggaacat cttctgggtg ctggcgcttt ggggcgtggt gcagtggctg
     8761 gcgttaagtg cgctgaacca gtggctggca cctgagcgcg atttaagcaa tgcctccaat
     8821 gccgcttatg ccgattccac cggtgagttc ctgcacggga tgatcaccgc cagcaccagc
     8881 ttgtggtatc tgtatgcgtt aattgtctat ttcgtggtat gtaaaatttt tagccgtctg
     8941 gcgctgccac tattcgcctt gtttgtactg ctgagtgtgg cggttaattt cgttcccacg
     9001 ccgtggtggg gaatgaacag tgtgatccgc aatttgcctt attacagcct tggcgcatgg
     9061 tttggcgcaa caataatgac ctgtgttaaa gaggtaccgt tgcgccgcca tctgctgatg
     9121 gcttctttgc ttaccgttct ggcggtcggt gcctggttgt ttactatctc gctgctgttg
     9181 tcgctggtat cgattgtggt gatcatgaag ctgttttatc agtacgagca acgattcggt
     9241 atgcgctcca ccagcctgct gaatgtgatt ggctccaaca ccattgctat ctacaccacc
     9301 catcgcattc tggttgaaat attcagctta actctgcttg cgcaaatgaa cgcagcacgc
     9361 tggtcgccgc aagtcgaact gacactcctg ctggtttatc cctttgtcag tttgttcatc
     9421 tgtactgttg cgggcttgct ggtaagaaaa ctttcacagc gtgcattcag cgatctgttg
     9481 ttctccccgc cttcactgcc cgctgccgtc agttactccc gctaagccat cgccccagcc
     9541 ggggcgattc aatttgctaa accatgtcga ttacggataa tgaacagcat tgcttatcag
     9601 agatatgccc ccatatgttg aggcatatcc taacgagaat ctgacaaccg ttatgcctga
     9661 acaatatcgt tatacgctgc ccgtcaaagc gggtgagcag cgtctgctgg gcgagttaac
     9721 cggcgcagcc tgtgcaacgc tggtagcgga aattgccgaa cgtcacgccg gtccggtggt
     9781 actcattgca ccagatatgc aaaatgctct gcgtttgcat gatgaaatca gtcagttcac
     9841 cgatcagatg gtgatgaatc tggcggactg ggaaactctt ccctacgaca gcttttcgcc
     9901 tcatcaggac attatctcct cgcgcctttc caccctttac cagctaccga cgatgcagcg
     9961 tggcgtactg attgttccgg tgaatacgct tatgcagcgc gtttgcccac acagttttct
    10021 ccacggtcat gcgctggtga tgaaaaaagg tcagcgcctg tcacgagatg cattacgaac
    10081 ccaactggac agcgccggtt atcgccatgt tgaccaggtg atggagcacg gcgaatacgc
    10141 cacgcgcggc gcgttgctgg atctcttccc gatggggagt gagctgcctt atcgtcttga
    10201 tttctttgat gatgaaatcg acagcctgcg ggtgtttgac gtcgacagcc agcgcacgct
    10261 ggaggaagta gaagcgatca atctgctgcc cgcgcacgaa tttccgaccg ataaagcggc
    10321 aattgaactg ttccgcagcc agtggcgcga taccttcgaa gtgaagcgcg atccggaaca
    10381 tatttaccag caagtgagta aaggcacatt acctgccggg atcgagtact ggcagccatt
    10441 gttcttcagc gaaccactgc cgccgctgtt cagttatttc cctgccaata ccttgctcgt
    10501 gaatactggc gatctggaaa ccagtgccga acgtttccag gctgacacgc tggcgcgttt
    10561 tgagaatcgc ggcgtcgatc cgatgcgccc gctgttgcca ccacaatcgc tctggctgcg
    10621 ggtggacgag ctcttctcag agctgaaaaa ctggccccgg gtgcagctaa aaactaaaca
    10681 tttaccgaca aaagccgcga atgccaattt aggtttccag aaactgccag acctagccgt
    10741 tcaggcgcaa caaaaagcgc cgctggatgc gctgcgtaag ttcctcgaga ctttcgacgg
    10801 tccggtggtg ttctcggtag aaagtgaagg tcgccgtgaa gcgctgggtg aactgctcgc
    10861 acgaattaaa attgctccgc aacgcattat gcgtcttgat gaagccagcg accgtgggcg
    10921 ttatctgatg attggcgctg ccgaacatgg ttttgtcgat aaggtgcgta atctggcgct
    10981 gatctgcgaa agcgatctgc tcggtgaacg tgttgcccgc cgtcgtcagg attctcgccg
    11041 caccatcaac cccgatacac tgatccgtaa ccttgcggag ctgcatattg gtcagccggt
    11101 ggtccatctg gagcacggtg tcgggcgcta tgccggaatg accacgctcg aagcgggcgg
    11161 cattactggc gagtatttga tgctcaccta tgccaacgac gccaaactgt atgttccggt
    11221 gtcgtcactg catctgatta gccgttacgc aggtggcgcg gaagaaaacg ccccgctgca
    11281 taaacttggc ggcgatgcgt ggtcacgcgc gcggcagaaa gcggcggaaa aagtgcgtga
    11341 tgtggcggcg gaattgctgg atatctacgc gcaacgcgcc gccaaagagg gcttcgcgtt
    11401 taaacacgat cgtgagcagt atcagttgtt ctgcgacagc ttcccgtttg aaaccacgcc
    11461 ggatcaggcg caggccatta atgcggtact tagcgacatg tgtcagccgc tggcaatgga
    11521 tcgtctggtg tgcggcgatg ttggctttgg taaaacagaa gtggcgatgc gcgccgcttt
    11581 cctggcagta gataaccaca agcaggtggc ggtgttggtg cctaccaccc ttctcgcgca
    11641 gcagcattac gacaacttcc gcgaccgttt cgccaactgg ccggtacgta tcgaaatgat
    11701 ctcccgtttc cgcagcgcca aagagcagac gcaaatcctt gcggaagtgg cggaagggaa
    11761 aatcgatatt ctgatcggta cgcacaaact gctgcaaagt gacgtcaagt ttaaagattt
    11821 aggcctgctg attgtcgatg aagaacaccg cttcggggtg cgtcataaag agcgcattaa
    11881 agcgatgcgc gcgaacgtgg atattctgac gcttactgca acgccgatcc cacgcacgct
    11941 gaatatggca atgagcggaa tgcgtgacct gtcgattatc gccacgccgc ccgcccgtcg
    12001 tctggcagtt aaaacctttg tccgtgagta tgacagcctg gtggtccggg aggcgatcct
    12061 gcgtgaaatt ttgcgcgggg ggcaggttta ttatctctac aatgatgtgg aaaacatcca
    12121 gaaagctgcc gaacggctgg cagaactggt gccagaagcg cggatcgcca tcggtcacgg
    12181 gcagatgcgc gagcgcgaac tggaacgggt gatgaatgat ttccatcatc aacgtttcaa
    12241 cgtgctggtt tgtacaacca ttatcgaaac cgggatcgac atcccgacag ccaacaccat
    12301 tatcattgaa cgcgcggatc acttcggtct ggcgcagctg caccagttac gcggtcgcgt
    12361 cggacgttca catcatcagg catatgcatg gctgctgacg ccgcatccaa aagcgatgac
    12421 taccgatgca caaaaacgtc ttgaagcgat tgcctcgctg gaagatctcg gtgcaggctt
    12481 tgcgctggca acgcacgatc tggagatccg cggcgcgggt gaactgcttg gcgaagaaca
    12541 aagtggctca atggaaacca tcggtttctc gctgtatatg gagttgctgg aaaacgccgt
    12601 cgatgcactg aaagccggac gcgagccgtc gctggaagat ctcaccagcc agcaaacaga
    12661 agtcgagctg cggatgccgt cgctattgcc agatgatttc attcctgacg tgaatacgcg
    12721 tttgtcgttc tataaacgta ttgccagcgc caaaacggaa aacgaactgg aagagatcaa
    12781 agtcgagctt atcgatcgct tcggcctgct gccggatccg gcgcgtaccc tgctggatgt
    12841 tgcccgtctg cgccagcaag cgcagaaact ggggatcagg aagctggaag gtaatgagaa
    12901 aggcggggtg atcgaatttg ccgagaagaa tcacgttaat ccggcctggt tgattggttt
    12961 gctgcaaaaa cagccgcagc attaccgcct tgatggtccg acgcgcctga aatttattca
    13021 ggatttgagt gagcggaaaa cgcgtatcga atgggtacgc cagtttatgc gtgaactgga
    13081 agagaacgcg atcgcttaat ggtaaatcgg gggcgtttct gcgcccccat cggcaacatt
    13141 tacaaattct ttgcacttcc ctgcactatc cgacactgtc accatccata attcaggctt
    13201 atctgtttat tacaataacc ttatatttat tatggatttt tggtgatgat caaaacgcgt
    13261 ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctggc gctaccggca
    13321 aaagccaaca cctggccgct gccgccagcg ggcagtcgtc tggttggcga aaacaaattt
    13381 catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata taacgtcggc
    13441 tttctcgctc tgttacaggc taaccccggc gttgatcctt acgtaccgcg cgcgggcagc
    13501 gtgttaacga tcccgttgca aaccctactt ccagacgcgc cgcgcgaagg cattgtgatc
    13561 aacattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt aaccgtgtat
    13621 ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt gaccaccgtt
    13681 tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acacccgcgc ccgttataaa
    13741 gcacagggaa ttgagttgcc tgcggtagtg ccggctggac cggataaccc aatgggccat
    13801 catgcgattc gtctggcggc ctatggcggc gtttatttgc ttcatggtac gaacgccgat
    13861 ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga cgatatcaaa
    13921 acactcttta gccaggtcac cccaggcacc aaagtgaata tcatcaacac tccgataaaa
    13981 gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct gtcagagaag
    14041 attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca atcatttaaa
    14101 gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg ttccgggatg
    14161 ccggtggatg tccgccgtca tcaagtgagc ccacaaactc tgtaaatcct ggataaaaaa
    14221 aatccccgcg acacggcatg tcgcggggaa aaaatcagca gtagcaggca ttaatgaggg
    14281 ttaatgctta tttataaatt actgctgttc catggagggt attcggaccg gttacagaag
    14341 taatacggaa agattttgca cccatctcat ccgctttttg cgccagctgc tcttccagcg
    14401 atcccagatt tgtccccgct ttagcactga ttgtaccgac tttttgttgg ccttctggcg
    14461 ttgactgaac ttcgacagcc gcaaagctgg caaatgacat ggagcttaaa atcgccgcag
    14521 cgatgagggt ttttacgttt ttcataatag tggccttatg cagatgaatg acgtcgcaag
    14581 caattactta acgatcgata actaaatgac agatgtgatc tggatcacat acaagatatt
    14641 ttttataaca atcattaatt aatttaaaaa tctttaaatt tcagttaaat agaaagcaat
    14701 tatttttatg ggatctggac tggtgaacgg aggtgattat ttttataata atctttcatt
    14761 cacagtcacc aggtacaacg gcatcatggc aactgactca acacaatgtg taaaaaaaag
    14821 ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca tgaaattgtt
    14881 ctggcaacac ggttatgaag cgacttctct tgcggacctc gtcgaagcga ccggagccaa
    14941 agcgcccacg ctatacgcgg aatttaccaa caaagagggg ttatttcgtg ccgttctcga
    15001 ccgctatatc gatcgttttg ccgctaagca tgaagcacag ctgttttgtg aagagaaaag
    15061 cgtggagtct gcgctggctg actattttgc tgccatcgcc aactgcttta ccagcaaaga
    15121 caccccggca ggctgcttca tgatcaacaa ctgcaccacc ctctccccag attcaggaga
    15181 tatcgccaat acgttgaaat cacgccatgc gatgcaagag cgcactttgc agcagttttt
    15241 atgtcaacga caagcgcgcg gggaaatccc gacccactgt gacgtgacac atctggcaga
    15301 attccttaat tgtattattc aggggatgtc gatcagcgca cgcgaaggtg catcgctgga
    15361 aaaactaatg cagattgccg gaacgacttt gcgtttatgg cccgaactgg tgaaataagt
    15421 cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa attgcagagt acagccttgt
    15481 tatactttgt tattcaaaat cagttgccca ttactatcta gcgggatctg cgtacccgga
    15541 tcgcggtcca tgcggatttt gccctgctga tcgccaatct tataggtcac atcataaccg
    15601 agcatttttt ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt cgtgtaagta
    15661 tcgctttcct ggagagagcc ctggatctgg ttaccggcat atccaccacc cagcgccccc
    15721 acaacagtgg cgacatcttt accgcgacca ccgccaaact gatgccctat cacgccgcca
    15781 gcaacagcgc cgagcaccga cccggtaatg cgattttcat cctgcaccgg tcgacgatgg
    15841 gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt gattggggtt
    15901 gcagaaacaa cctgagcgta ttgcgggcca cgttcaaaca cgttcagact ggccactgcc
    15961 gctacgccca gcgcagctgc gacaccaatc ccgatacccg ccaacattga tttattcacg
    16021 ggaacacctc cttctttgca gttatgccaa tcatgcaagc gaaggagaaa agcagctatc
    16081 agacagaaga tgaaaagcgc gggaatggct ggaaatactt aatgattcgg aggagtctga
    16141 aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt accatttgtc
    16201 cagggcggat gaggcgttta tgccacatcc gccagtgtac gtcgattaat gcaacttcaa
    16261 acgcggacgg ataacgcggt taatactccc caccagcatc attaatccgg ttttaaagta
    16321 accatgcagc gcaatctgat gcattcggta tagcgagata tatacaaagc gcgcaattcg
    16381 tccttcaatc atcattgagc cgcgcgtcaa gttacccatc aggctaccaa cggtggagaa
    16441 gttcgacagc gataccagtg aaccatgatc tttatactga taatttttca gcggcttacc
    16501 gtttatctgc gccagaatat tgttcattgc gcaagtcgcc atctggtgtg cagcctgagc
    16561 acgcggcgga acaaagcccc cttccggacg cgtgcatgac gcgcagtcgc caatagcgta
    16621 aatgtctgga tcgtgggtgg tttgcagcgt cggttccacc accagctggt tgatacggtt
    16681 agtttcaaga ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg ctgcccacac
    16741 catcagatca gcctcaatat attcaccatc tttagtgtgc aggccgcctt catcagcact
    16801 ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc gttaactcac tatgggccgc
    16861 agcagagatg cgtggcggta atgcaggcaa aatacgttct cccgcttcta ctagcgttac
    16921 gttcagggct tcgttggtca gacctttata accgtagctg tgcagttgct tgaccgcgtt
    16981 atgcaactca gcggagagtt ctacacccgt cgcgccgcca ccgacaatcg caatgttcac
    17041 tttgccattc gcgcccaggt tggcggagta tttcaggaac agattcagca tctcctggtg
    17101 gaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag ttctctttga cgcctggcgt
    17161 attgaaatcg ttagaggtgc tacccagcgc catgaccagg gtatcatagg cgattttacg
    17221 ttccggaacc agcagttcac ctttctcgtc gcgtagttct gcaatagtga ttgttttcgc
    17281 ttcacgatca atatcaatga cggaacccaa ctggaactgg aaaccatgat tgcgcgcatg
    17341 ggccagatag ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca cttcgtgcag
    17401 cagcggtttc cacaggtggc tgtggttacg atcgaccagc gtaattttgg cttttttctt
    17461 acgtcccagc ttatgcccca gctgtgttgc catttccagc ccaccagcac cgccgccgac
    17521 aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc ttaaatttat taaccaattg
    17581 ttaattaatg gttattaaca tagctcttaa ttaacaacag gttacagcaa tgaaaatgtt
    17641 cttcgctatt gagaataaca tgaatggtgc attggtcata ccaaaattga tgttaatcaa
    17701 gttttgttgc tgaaaagata ggcgacttta ccgggtgaga tcaaaaaata gagtttattg
    17761 tttattattt gagcgaattt gtgagagtga tatagagtga ggggtgtatg cagggcgttc
    17821 ggtaggcctg ataagaggcg catcaggcat tgatgctcga ctgtcggatg cggcgtaaac
    17881 gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta cctgatgcga
    17941 cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgcaaatgcg gggagatatt
    18001 cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat gcaactcttc
    18061 agatgtccgc tggctgttaa gcgcttcatc attacgcgac aaaatcacca ggcaacgatc
    18121 gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat actcttccgg
    18181 gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga agatcacctg
    18241 gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccagac caacgccgca
    18301 aatcagcgga cgctcgtcga cgttcagttg caacattttg tccacttctt taagcagatg
    18361 ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat ccgggtcaat
    18421 aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa aaccgtgtaa
    18481 atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc tcttcctgcc
    18541 agcgttcatg taactgattc agacgggtgc tgctcgcctt ccagcgagcc gagtccatca
    18601 gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct gccttgatcg
    18661 gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc aggatcatat
    18721 cgcaacctgc gtccagtgat gcctgcccgc gttcggcata actgcccata atcgcggcac
    18781 cttccatcga taaatcgtca gagaaaatta caccgtcaaa acacagttcc tgacgcaaaa
    18841 cggttttcag ccagtaagaa gaaccgctcg ccggacgcgg atcaacatca ctgtagatca
    18901 catgcgcagg cataatggcg tcgagtttat tttcgcggat taacgtgctg aaaaccgaca
    18961 tatctttggc acgaatttcc gcttgcgggc gtgggtcgca cggcgtctct ttgtgtgaat
    19021 ctgcagttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt ccggcttcat
    19081 gcatcccatc aataaaccga ctagcgattg ccagggcttt ttctgggtcg gcatgataag
    19141 aacgctcgcc aatcgccgcg ctgatatgtc ctacatccag cactggcgca aagctgatat
    19201 caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc gccagtttgc
    19261 cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag cgggtaaaac
    19321 cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc agatgattgc
    19381 gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca tgatagttac
    19441 gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc tcttccgcgt
    19501 ccagttcata accttcgaca tccaacatta ctggacccac actgctctcc ttattgtttt
    19561 attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc gccggtttgt
    19621 cgccagcggt actcaaacca ccctgctttg agcatcagca gccagggaaa ccatcgcctg
    19681 acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc attgaccaat
    19741 tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc ggtatcacca
    19801 tctccggcat actcccagtc gatgagtttt aaccctgatg cgctatgcac taaatttccg
    19861 gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg cgctttgcgc
    19921 agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct ttgctgccag
    19981 tacagttcca gtaacggcaa cagcgttatt cgccagccaa aacgtggttg ttgatgtaga
    20041 taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac cgcgccgggc
    20101 agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc aatgcttgcg
    20161 ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc atcaggatcg
    20221 tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact accgccacta
    20281 agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga cagcaattcg
    20341 tcgcgcgtga tgggattatt actgcgaaac ggcaccttta cctgaccaga taatttcgcc
    20401 cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc cagaggcgct
    20461 ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt tactacgggt
    20521 gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca gttgctgggc
    20581 ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt cggtcgcttc
    20641 tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca ggacgctacc
    20701 cgcagtcacc ccgtcagccc caagcatctt actgactatc ggctgcattg cgccattcca
    20761 gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct caattgggcc
    20821 tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt gtggctcggc
    20881 tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt gtcccacaca
    20941 cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt ttgtcatcaa
    21001 gattcacccc ttacaaatat agataaagtc tgactttgtg cgcccccaga aaattggcgc
    21061 tgccgtacag cgttaccgcc gaatgtgcgg gaatggtaac gctgcgtggc ctttccagag
    21121 gatgcatctc cagccctctg gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt
    21181 tttgcctttc gttataaagc gttgaggatg ctgaaggttg aatatcagac gtcgaaagga
    21241 cgggcttttc tgcactgatg cccgcagcca ataaagatga ctccatcacc agcgattgct
    21301 catcattcac cggaatttcc ggatgtgaac gacagcccac cagcagtaac aacaccagag
    21361 acaccagccc aaagcatcct tttctcatcg ttacagacct ttatgcgcca gcattggtcc
    21421 cagcggacgg ccacccaaca agtgcatatg gatgtggtaa acctcttgtc cgccatggcg
    21481 gttggtattc atgatcagac gatagccatc ttcggcaata ccttcttgct cagcaatttt
    21541 tgccactacg gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaga cgtcgttcac
    21601 agtcggaatg aggatattcg gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc
    21661 gcgaaacgcc gttaccagat catcctggta gacgatatcg gaggggatct cacgacgaat
    21721 aattttgctg aatatagttt cttctgccac gacattttcc tttttcaaat agccatgcgt
    21781 tgcgccatgc tacgccgcgc cacactgcga gtatagagta tggtcgagtt aaacctgctc
    21841 tttcaacctt aactcacgat ttcttaagca aaaaatcgtt ttgctgccgt atatatcgcc
    21901 attattccca tttctgcctg cgaatgcgct gaaaagacat tctctgtttg gtttacacga
    21961 caaatatgaa tgcgtatatt tctcatttgc atttacaaac aaaattattc gcacactaaa
    22021 tataatcgct catcaccctc ctccggc
//
LOCUS       DAJVTY010000059        20902 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVTY010000059 DAJVTY010000000
VERSION     DAJVTY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20902
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.059"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(162..707)
                     /gene="hemG"
                     /locus_tag="OQQ66_001982"
     CDS             complement(162..707)
                     /gene="hemG"
                     /locus_tag="OQQ66_001982"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1904926.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(719..2170)
                     /gene="trkH"
                     /locus_tag="OQQ66_001983"
     CDS             complement(719..2170)
                     /gene="trkH"
                     /locus_tag="OQQ66_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1904927.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2209..2823)
                     /locus_tag="OQQ66_001984"
     CDS             complement(2209..2823)
                     /locus_tag="OQQ66_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1904928.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2823..4154)
                     /gene="pepQ"
                     /locus_tag="OQQ66_001985"
     CDS             complement(2823..4154)
                     /gene="pepQ"
                     /locus_tag="OQQ66_001985"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1904929.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4344..6540
                     /gene="fadB"
                     /locus_tag="OQQ66_001986"
                     /pseudo
     CDS             4344..6540
                     /gene="fadB"
                     /locus_tag="OQQ66_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
     gene            6550..7713
                     /gene="fadA"
                     /locus_tag="OQQ66_001987"
     CDS             6550..7713
                     /gene="fadA"
                     /locus_tag="OQQ66_001987"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1904930.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7905..8606)
                     /gene="fre"
                     /locus_tag="OQQ66_001988"
     CDS             complement(7905..8606)
                     /gene="fre"
                     /locus_tag="OQQ66_001988"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1904931.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8652..10145)
                     /gene="ubiD"
                     /locus_tag="OQQ66_001989"
     CDS             complement(8652..10145)
                     /gene="ubiD"
                     /locus_tag="OQQ66_001989"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1904932.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10312..10800
                     /gene="rfaH"
                     /locus_tag="OQQ66_001990"
     CDS             10312..10800
                     /gene="rfaH"
                     /locus_tag="OQQ66_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1904933.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMIPLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10797..11579)
                     /gene="tatD"
                     /locus_tag="OQQ66_001991"
     CDS             complement(10797..11579)
                     /gene="tatD"
                     /locus_tag="OQQ66_001991"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1904934.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAVQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11621..12397)
                     /gene="tatC"
                     /locus_tag="OQQ66_001992"
     CDS             complement(11621..12397)
                     /gene="tatC"
                     /locus_tag="OQQ66_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1904935.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12400..12915)
                     /gene="tatB"
                     /locus_tag="OQQ66_001993"
     CDS             complement(12400..12915)
                     /gene="tatB"
                     /locus_tag="OQQ66_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1904936.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12919..13188)
                     /gene="tatA"
                     /locus_tag="OQQ66_001994"
     CDS             complement(12919..13188)
                     /gene="tatA"
                     /locus_tag="OQQ66_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1904937.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTVKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13267..14907)
                     /gene="ubiB"
                     /locus_tag="OQQ66_001995"
     CDS             complement(13267..14907)
                     /gene="ubiB"
                     /locus_tag="OQQ66_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1904938.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14904..15509)
                     /gene="ubiJ"
                     /locus_tag="OQQ66_001996"
     CDS             complement(14904..15509)
                     /gene="ubiJ"
                     /locus_tag="OQQ66_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1904939.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15523..16278)
                     /gene="ubiE"
                     /locus_tag="OQQ66_001997"
     CDS             complement(15523..16278)
                     /gene="ubiE"
                     /locus_tag="OQQ66_001997"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1904940.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16373..17800)
                     /gene="rmuC"
                     /locus_tag="OQQ66_001998"
     CDS             complement(16373..17800)
                     /gene="rmuC"
                     /locus_tag="OQQ66_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1904941.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17941..18702)
                     /gene="udp"
                     /locus_tag="OQQ66_001999"
     CDS             complement(17941..18702)
                     /gene="udp"
                     /locus_tag="OQQ66_001999"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1904942.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18964..19776
                     /locus_tag="OQQ66_002000"
     CDS             18964..19776
                     /locus_tag="OQQ66_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1904943.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20170..20830
                     /gene="hcp"
                     /locus_tag="OQQ66_002001"
                     /pseudo
     CDS             20170..20830
                     /gene="hcp"
                     /locus_tag="OQQ66_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5106 a         5638 c         5245 g         4913 t
ORIGIN      
        1 ttttgctttt ctctgttgag attctcagga gaaccccgct gacccggcgg cgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagaggaaat
      121 ttaaaataat tttctgaccg cgcacttttt attctttatg cttatttcag cgtcggtttg
      181 ttggttaact gggcgatttc tcgggcgaaa ttcgccacct gctcccaatc ggtatagaca
      241 acttctttgc gcgtatccgt ttcaccgcct gacatcttca taatcagctt aatcataaaa
      301 cggtcgtacc agcgataacg tgggtaacgc agcgccccgg caatgaccgc gcagcgatcg
      361 ggacgccatt gtgaactcat cagaaacttg cgcgcgtagc tgttggtctg tggagtacgc
      421 ttctccggtt tgcgcgccac caggtttacg gaataaaagg cgctcggcat cgaattcagt
      481 cgcgtcgcat gttttttgac aaattcctgg aacgcagagt ggtagtgacc atagcgaata
      541 gaagcaccaa tgaccacacg gtcatagttt tcccattgtg gttcttcaat gcggtgcaca
      601 ttggcgacat ccgtctggat ccccagttct ttcagttccg aagccaggta ggaggcaatc
      661 tcgcgcgttt gtccgtccct tgttgagaaa agaattaatg ttttcacgta ttactccatc
      721 attcacgcca gaaagtcggg gtaaagagca ccagcaatgt aaagacctcg agacgaccaa
      781 acagcatgtt ggcaatcagg atccatttag ccaccgggtt catactggta aagttatcag
      841 caaccacgcc aagccctggc cccaggttat tcaatgtcgc aacaactgag gcaaaggcag
      901 aaaaatcatc cacgcccgtg gcgataatcg ccagcatact gacaataaac accaatgcat
      961 aggcggagaa aaatccccaa acggcttcga ggatacgttc cggcagtgcg cgattcccca
     1021 gcttaatgct atagacggcg ttcggatgca ccagtcgttt cagctcacgg ttcccctgct
     1081 taaacagcag caggatgcgg atcactttca ggccaccgcc cgttgacccg gcgcaaccgc
     1141 cgataaaagc tgaacataaa agcagtaccg gcaaaaagag cggccagcgg gcaatgctgt
     1201 cagtcgtaaa cccggcggtt gtcgccatcg ataccacctg gaaaaacgcc tggttaattg
     1261 tcatcagcgc cgaactgtag acattatgaa accacagtac gagtgtacaa ataaccacca
     1321 gcgtaaactg tacgccgata aacatgcgaa attccggatc gcgccaataa accttcagac
     1381 tacgcccact taacagtgaa aagtgcagac cgtagttaca accggagatc agcaggaaga
     1441 tagcaatgat agtgttaata gtcggactgt cgaaataacc gatactggca tcatgtgtcg
     1501 agaagccgcc aatagcgata gtcgcaaagc tatggccgat ggcatcaaag gcatccattc
     1561 cggcaaacca caacgccagc gcacaggcga cggtcagcaa gacataaatc aaccacaggg
     1621 ttttcgccgt ttccgcaatt cgcgggcgca ttttgttatc tttcagcggg ccgggcattt
     1681 ctgcgcgata gagctgcatc ccacccacgc cgaggatagg cagtatcgca accgctaaca
     1741 cgatgatccc catcccgcca aaccattgca gcatctggcg ataaaagagg atggcatgag
     1801 ggagcgaatc cagccccacc agcgtagtgg ccccggtggt ggtcaggcca gagaaagatt
     1861 caaaaaacgc atcggtaatc gtgaggttcg ggctttccga gaagataaaa gggagcgcac
     1921 cgacactacc cagcacggtc caaaacagca ccactatcag aaacccctca cgggatttca
     1981 gttcgccctt ctctttgcgg ttcggccacc acagcataga gccaatggcg agggcgacaa
     2041 aaaaggtctg ggtaaaagcg cggcccgctc catcccggta gatgagtgct accagcccag
     2101 ggataatcat ggtccctgaa aataagatga ccagtagtcc aacgattcgg gtaatggcgc
     2161 gaaaatgcat ctctgccgct tccttagttc tgcaaaatga agtggggatt attcttcaat
     2221 cgctaacaat tgcaatgaac cacggctaaa atccgccagc tttgcggaaa attcagccac
     2281 tttcgccgcc ggaagcgcca cccgcagcag aacgaatgcc tgataatcac tgttgataat
     2341 tttgccgtca cactgcccca gcaacgcttc aatgccggtt aactgactat attcacattg
     2401 caaagtatat tcggttaatg gcgtcttgcg ttgggtcgtt agctggcgca gcgcctgatt
     2461 cacgccgccg ccatacgctt tcactaaccc accggtgcca agcaatatgc cgccgtagta
     2521 gcgcactacc acagcggtaa tttccccgat gccgctgccc attagctggg cgagcatcgg
     2581 tttacctgcc gttcccgccg gctccccgtc gtcagagaaa cccagctgtt gagaatcatc
     2641 cggcgcaccc gccacccacg ccacgcaatg gtggcgggca tcggggtgtt ctgcccgcac
     2701 cgattcaaca aacgctttcg ccgcctcaac gccatcggta tgcgccaaca tcgttatgaa
     2761 gcggctcttt ttgatctctt caacgaccgt gaccggtgcc gcaggaatta accagctttc
     2821 catcacgcca gtttcagatc ccgggtcatg ttttccacgt tattttcgtg gatcaccacg
     2881 ttgtcttcga tacgaatgcc gctgaacggt ttcagtgctt caattttctg ccagttgaag
     2941 tgcttgctga actgcccttc acgccacggt gccagtagcg attcgatgaa gtagataccc
     3001 ggttcgatgg ttaacaccat gcccggctgg agaatacggg tgcagcgcag gtacggatat
     3061 tttgccggtg ccgcgaggtg tgtaccgcta tcatcctgca taaaaccggc tacgtcatgc
     3121 acctgcaggc ccagcggatg gccgataccg tgcggcataa acggtccggt gagatcgttt
     3181 tcgaccatcg cttcttcact catatcggtg atgatttgat gtttacgcag caatttggcg
     3241 atgcgctgat ggaactggag gtggtaatcc acatagctga cgccagcttt catggtagcg
     3301 atcagcgcaa gttgttcatc atttacgtct ttcaccagct gtgcgtagtc gttgtcgctt
     3361 tttgccgacc aggtacgagt cagatcagcc gcatagccgt tatattcggc cccggcatcc
     3421 agcaggaagc tgcgcatctc ttccggtgcc tgatgatcca gtttggtgta atgcagcacc
     3481 gaagcgtgtt cgttaagcgc cacaatgttg ctgtaaggta cgtcggtatc acgatgaccg
     3541 gtcgcggtca gataggcgat gttgatatcg aactcgctca tgccagaacg gaacgcttct
     3601 tctgccgcac gatgaccgtt gaccgccatt ttctgcgctt cacgcataca ggccagttcg
     3661 tactcggttt tgaaggagcg gtagtaatgc aggtagtcga tcaccccttt cgggttgatg
     3721 ttgctggcct caatacccag ttgcagcgca cgttccggca ccggaccgat ataaccgata
     3781 ttgccgcgcg cagcgggcag caggctacca atgccatcgg ctttcggcag tgcgatcact
     3841 tccacatctt cagtccagaa ggaggtcggc agcggttcga cgttgtgcca gtaatcaacc
     3901 ggcagataga accacagttt cggcttgttc acgccatcca ccagcaacca gcagtttggc
     3961 acctgagtta ccggcaccca cgctttgaat tgcgggttca ctttaaacgg atagggatga
     4021 tcgtcgagaa aaacgttgaa cagctcgccg gagtgaataa gtaatgcatc cagcttgaag
     4081 cgcgccagcg catcgcgagt ccgttcttgt aaggtagcta tatgattttt atagagcgag
     4141 gccagtgatt ccatttttta cccttctgtt tttttgacct taagtctccg catcttagca
     4201 catcgttcat ccagagcgtg atttctgccg agcgtgatca gatcggcatt tctttaatct
     4261 tttatttaca tgtttttaac acaaaataca cacttcgatg catctggtac gaccagatca
     4321 ccttgcggat tcaggagact gacatgcttt acaaaggcga caccctgtac cttgactggc
     4381 tggaagatgg cattgccgaa ctggtatttg atgccccagg ttcagttaat aaactcgaca
     4441 ctgcgaccgt cgccagcctc ggcgaggcca tcggcgtgct ggaacagcaa tcagatctaa
     4501 aagggctgct gctgcgttcg aacaaagcag cctttatcgt cggtgctgat atcaccgaat
     4561 ttttgtccct gttcctcgtt cctcgttcct gaagaacagt taagtcagtg gctgcacttt
     4621 gccaatagcg tgtttaatcg cctggaagat ctgccggtgc cgaccattgc tgccgtcaac
     4681 ggctatgcgc tgggtggtgg ctgcgaatgc gtgctggcga ccgattatcg tctggcgacg
     4741 ccggatctgc gcatcggtct gccggaaacc aaactgggca tcatgccggg ctttggcggt
     4801 tctgtacgta tgccacgtat gctgggcgct gacagtgcgc tggaaatcat tgccgccggt
     4861 aaagatgtcg gcgcggatca ggcgctgaaa atcggtctgg tggatggcgt agtcaaagca
     4921 gaaaaactgg ttgaaggcgc aaaggcggtt ttacgccagg ccattaacgg cgacctcgac
     4981 tggaaagcaa aacgtcagcc gaagctggaa ccactaaaac tgagcaagat tgaagccacc
     5041 atgagcttca ctatcgctaa agggatggtc gcacaaacgg cggggaaaca ttatccggcg
     5101 cccatcaccg cagtaaaaac cattgaagct gcggcccgtt ttggtcgtga agaagcatta
     5161 aatctggaaa acaaaagctt tgtcccgctg gcgcacacca acgaagcccg cgcactggtc
     5221 ggcattttcc ttaacgatca atatgtaaaa ggcaaagcga agaaactcac caaagacgtt
     5281 gaaacaccga aacaggccgc tgtactgggc gcaggcatca tgggtggcgg cattgcttac
     5341 cagtctgcgt ggaaaggcgt accggttgtc atgaaagata tcaacgacaa gtcgttaacc
     5401 ctcggcatga ccgaagccgc gaaactgctg aacaagcagc ttgaacgcgg caagatcgat
     5461 ggtctgaaac tggctggcgt gatctccacg atccacccaa cgctcgacta cgccgggttt
     5521 gaccgcgtgg atgttgtggt agaagcggtt gtcgaaaacc cgaaagtgaa aaaagccgta
     5581 ctggcagaaa ccgaacaaaa agtacgcccg gataccgtac tggcgtctaa cacttcaacc
     5641 attcctatca gcgaactggc caacgcgctg gaacgcccgg aaaacttctg cggaatgcac
     5701 ttttttaacc cggtacaccg gatgccgttg gtagaaatta ttcgcggcga gaaaagctcc
     5761 gacgaaacca tcgcgaaagt tgtcgcctgg gcgagcaaga tgggcaagac gccgattgtg
     5821 gttaacgact gccccggctt ctttgttaac cgcgtgctgt tcccgtattt cgccggtttc
     5881 agccagctgc tgcgcgacgg cgcggatttc cgcaagatcg acaaagtgat ggaaaaacag
     5941 tttggctggc ctatgggtcc ggcatatctg ctggatgtcg tgggcattga taccgcgcat
     6001 cacgctcagg ctgtcatggc agcaggcttc ccgcagcgga tgcagaaaga ttaccgcgat
     6061 gccatcgacg cgctgtttga tgccaaccgc tttggtcaga agaacggcct cggtttctgg
     6121 cgttataaag aagacagcaa aggtaagccg aagaaagaag aagacgccgc cgttgaagac
     6181 ctgctggcag aagtgagcca gccgaagcgc gatttcagcg aagaagagat tatcgcccgc
     6241 atgatgatcc cgatggtcaa cgaagtggtc cgctgtctgg aagagggcat tatcgccact
     6301 ccggcggaag cggatatggc gctggtctac ggcctgggct tccctccgtt ccacggcggc
     6361 gcgttccgct ggctgaacac cctcggtagc gcaaaatacc tcgatatggc acagcaatat
     6421 cagcacctcg gcccgctgta tgaagtgccg gaaggtctgc gtaataaagc gcgtcataac
     6481 gaaccgtact atcctccggt tgagccagcc cgtccggttg gcgacctgaa aacggcttaa
     6541 ggagtcacaa tggaacaggt tgtcattgtc gatgcaattc gcaccccgat gggccgttcg
     6601 aagggcggtg cttttcgtaa cgtgcgtgca gaagatctct ccgctcattt aatgcgtagc
     6661 ctgctggcgc gtaacccggc gctggaagcg gcggccctcg acgatattta ctggggttgt
     6721 gtgcagcaga cgctggagca gggttttaat atcgcccgta acgcggcgct gctggcagaa
     6781 gtgccgcact ctgtcccggc ggttaccgtc aatcgcttgt gtggttcatc catgcaggca
     6841 ctgcatgacg cagcacgaat gatcatgact ggcgatgcgc aggcatgtct ggttggcggc
     6901 gtggagcata tgggccatgt gccaatgagt cacggcgtcg attttcaccc cggcctgagc
     6961 cgcaatgtcg ccaaagcggc gggcatgatg ggcttaacgg cagaaatgct ggcgcgtatg
     7021 cacggtatca gccgtgaaat gcaggatgcc tttgccgcgc ggtcacacgc ccgcgcctgg
     7081 gccgccacgc agtcgggcgc atttaaaaat gaaatcatcc cgaccggtgg tcacgatgcc
     7141 gacggcgtcc tgaagcagtt taattacgac gaggtgattc gaccggaaac caccgtggaa
     7201 gccctcgcca cgctgcgtcc ggcgtttgat ccagtaaacg gtacggtaac ggcgggcaca
     7261 tcttctgcac tttccgatgg cgcagctgcc atgctggtga tgagtgaaag ccgcgcccat
     7321 gaattaggtc ttaagccgcg cgctcgtgtg cgttcgatgg cggtcgttgg ttgtaaccca
     7381 tcgattatgg gttacggccc ggttccggcc tcgaagctgg cgctgaaaaa agcggggctt
     7441 tctgccagcg atatcggcgt gtttgagatg aacgaagcct ttgccgcgca gatcctgccg
     7501 tgcattaaag atctcgggct aatggagcag attgacgaga agatcaacct caatggtggt
     7561 gcgatcgcgc tgggtcatcc gctgggctgt tccggtgcgc gtatcagcac cacgctgctg
     7621 aatctgatgg aacgcaaaga cgttcagttt ggtctggcga cgatgtgtat cggtctgggt
     7681 cagggtattg cgacggtgtt tgagcgggtt taaccacttg ccggatgcgg cgtgaacgcc
     7741 ttatccggtc taccgatcct gcaccaatgt aggcctgata agacgcgcca gcgtcgcatc
     7801 aggcatcgtg caccgattgc cggatgcgac atagatctaa atgccactga ataaagtttt
     7861 agttgccgtt cttcccgcct gtcaggggcg ggttttttta tatctcagat aaatgcaaac
     7921 gcatcgccaa acagacgatc ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca
     7981 attttcgcca tctcaaaacg tccggcaata tagatatcat gctctgccag cgtaccatga
     8041 tcctgcaaga ccgccgttaa cacggtgcca gtacgcccac gccagcccgc ttccggttgt
     8101 tcaaccaccg gcaccacacg caggccagga tgcttcagcg aaagcgcctc aagctcggag
     8161 agatcataaa gatgttgctc ctcacgccca ccccagtaaa tggtgatatc gcgattggga
     8221 ttacgcgcca acgccgtcaa taaaatcgag cgcgcatagg agaacccggt gccgcccgca
     8281 atcaaaatca tcggacgctc ttcatcatcg cgcagccacg cttctccgta gggaatatcg
     8341 accacgattt gataatcttt gaggatgcgg tccatgactg ctttcgcgta aaggttgatt
     8401 tcagaagcgc caatatgcag ctctataaac cctttttcat ccggcgtcga agccattgaa
     8461 aacggacgtt tgtcgcgctc atccatcact accatcaaat actgaccagc acgaaaagaa
     8521 aaggccgcgt ctggcacgat gcggacacga tatacggtat ccgtgatagc ttctaccgag
     8581 gtcactttac agcttaaggt tgtcatgcgc tttctctgtc ggatcgataa atagggcaaa
     8641 acaaacgcgc atcaggcgct tttaccgttg ttaaaaatag ccagttcatc ccagatggcg
     8701 tcaatatgcg cgacaacatc tggatctttt ttgatgggac gtccccattc acgctgggtt
     8761 tcccccggcc atttattcgt ggcatccagc cccatttttg aacccagccc ggagacaggc
     8821 gaggcaaaat ccagataatc aataggcgta ttttctacca gaacagtatc ccgcgccggg
     8881 tccatacggg tggtaatcgc ccaaatcaca tcgttccagt cgcgtgcgtt gacgtcatca
     8941 tcgcaaacga tcacaaattt agtgtacata aactggcgta agaacgacca gacgcccatc
     9001 atgacgcgct tcgcgtgtcc ggcgtactgt tttttgatcg tcactaccgc cagacgataa
     9061 gaacagcctt ccggcggcag gtaaaaatcg acaatttccg ggaactgttt ttgcagaatc
     9121 ggtacaaaca cttcgttcaa cgccacgccc agtaccgcgg gctcatctgg cggacgcccg
     9181 gtataggtgg aatggtaaat cgcatcttca cgctgggtaa tatgcgtcac ggtaaatacc
     9241 gggaaactat cgacttcgtt atagtaaccg gtgtggtcgc catacggccc ttccggcgca
     9301 gtttcgcctt gttcgatata cccttccagc acaatctccg cactggcggg cacttcaagg
     9361 tcattggaga tacacttcac cacttcggtc ttggtaccgc gcagcaatcc ggcaaacgca
     9421 tactctgaaa gtgtatccgg aacgggggtg actgcaccga gaatcgtggc gggatcggca
     9481 cccagcgcca cagaaaccgg gaaacgttcg cccggatgcg ccgcacacca ctcctgataa
     9541 tccagcgcgc cgccgcgatg cgacagccag cgcataatca gtttgttttt accaatcagc
     9601 tgctggcgat aaatgcccag attctgccgc tctttatgcg ggccgcgcgt cactgtcagc
     9661 ccccaggtaa tcagcggtgc ggcatcttcc ggccagcagg tcataatggg aatgcgattg
     9721 agatcgacgt catcgccaga gacgattttt tgttggcagg gcacaccacg cagccgcttt
     9781 gtcggcatgt tcaatacttg cttaaactgc ggcagtttat caaacaggtc gcggaaacct
     9841 tttggcggct ccggctcttt cagaaacgcc aataatttac caacttcacg cagcgccgaa
     9901 acatcttcct gtcccatgcc catcgccacg cgctttggcg taccgaacag gttgcacagc
     9961 accggcattg agtagccttt agggttttcg aacaacagcg caggcccacc ggcacgcaaa
    10021 gtgcggtcag caatttcagt gatttccaga tgcggatcca ccgggagcgt gatacgtttt
    10081 agctcaccct gctgttcaag cagcgtcaag aagtcgcgta aatcgttata tttcatggcg
    10141 tccattgtag cctcttaatc tacgcccatt atacggcgtt catctttgcg atgctgtaaa
    10201 tttgttaaat tagcgtgaac tctgacggta taacgcaaac cggggaatat aattaactta
    10261 gcgtaaagct tttgctatcc ttgcgccccg attaaacgga taagagtcat tatgcaatcc
    10321 tggtatttac tgtactgcaa gcgcgggcaa cttcaacgtg cccaggaaca cctcgaaaga
    10381 caggctgtga attgcctggc accgatgatc cccctggaaa aaatcgtgcg tggaaaacgt
    10441 actgcagtca gtgagccatt gtttcccaac tacctgtttg tcgaatttga tccagaagtg
    10501 attcatacca cgactatcaa cgcgacccgc ggtgtcagcc acttcgtgcg ctttggcgcg
    10561 tcgccagcga tagtcccatc ggcggtgatt catcagctat cggtatataa accgaaagac
    10621 attgtcgatc cggcaacacc ttatccgggt gataaggtga ttattaccga aggcgcgttc
    10681 gaaggctttc aggccatttt caccgaaccc gatggtgagg ctcgttccat gctattgctt
    10741 aatcttatta ataaagagat taagcacagt gtgaagaaca ccgagttccg caaactctaa
    10801 aacgcaatcc caaacagtgt tttgacattg gcatccgtgg tggcagccag ccatgcggca
    10861 tcttctccac gccagtgcgc aatacgttgc aaaatatggt gcagatgggc tggctcgttg
    10921 cgccgggatg atggctttgg cgtgaaatcg cgcgggagca gatacggcgc atcagtttcg
    10981 atcagcaatt tctccgccgg aatcaacggt aacaattccc gcagctccag cccgcgtcgt
    11041 tcatcgcaaa cccaaccggt aatgccgata taaattccac gcgccacgca cgcctgcatc
    11101 tcttcgcgtg tgccggtaaa gcaatgaaga accgcaccag gcagtttatc cagccacggc
    11161 tccagcaatg tcataaaccg ctcgtgggca tcgcgacagt gcataaatac cggcatgttt
    11221 aattctgcgg caatgcgtag ctgagcaaca aaagcgcgtt cctgctcttc cggcgtcgaa
    11281 aagttgcggt taaagtcgag accacattca ccaatcgcca ccacttctgg ctgcacggcc
    11341 agctcaataa tcgcttcttc agtcgcagct tgccactggc tgctgtcgtg aggatgtacg
    11401 cccgccgttg accaacagga cgaatactga cgcgccagct tttgcgcctg ctggctttca
    11461 cgcaggttag tgccggtgat gagtagccca ttaactcccg cgtcaaaagc gcgcgctaca
    11521 acatcatcac ggtctttcgc aaattgcgaa ctggtcaaat taacgccgat atcaaacatc
    11581 ctgtactcca tatgacaacc gccctgacgg gcggttgaat ttattcttca gttttttcgc
    11641 tttctgcttc agcgtcgttt tcctcttccc gatttcgccc tttaccaacg taaaagcgtg
    11701 agaagaagac accgatttca aacagacagt acatcgggat cgccaatagc gtttgcgaga
    11761 agacatccgg cggcgtcagc aacatcccga caacgaatgc accaaccagc acatacgggc
    11821 gttttttgcg taagtcttct ggcgaggtaa tccccatcca gcacagcagc acaattgcca
    11881 ccggcacttc aaaggagaca ccaaacgcca taaacagcgc cataacgaag cttaaatagc
    11941 tggcgatgtc ggtggatacc tgcacccctt ccggcgcggt attggcaagg aagccaaatg
    12001 ccagcggaaa gaccacaaag taggcgaatg ccatgccgat ataaaacagc agagagctgg
    12061 aaaccagcag cggcaccacc aggcgacgtt catgcttata cagcgctggg gcgataaatg
    12121 cccacacctg atagagaatc accggcgctg acagaatcag cgacaccata aaggtcagct
    12181 tgatcggcgt aaagaacggc gaggccacgt cggtggcgat catcgttgaa ccttgcggca
    12241 actgcttgat caatggcgcg gataccaggt gatagatgtc attggcgaaa tagaccagac
    12301 acaggaatat cacgatcacc gcgataatgc agttcagcag acgcttacgc agctcaatta
    12361 gatgcgtgat aagcggttga gtatcttcta cagacatgtt tacggtttat cactcgacga
    12421 aggggaaggt gcagcggttt tcggttcagc gtccgcagca ggttttacca ccggctctgg
    12481 cgtggtttct ggcttctgtt ccggcgaact ggcctgcgtt tgtgcagcgg caggcgttac
    12541 gccctcatgc gcagcttcat tatctttcac caccgggtta tggatggtgt gcgcttcatc
    12601 gctcgccttt tctggatcgt ttgcaacgta ggaacgcttc atcgactccg cggcctggcg
    12661 tagttcatcc atcgacgctt tcagttcggg cgtcaggtta gtgaggctcg ccttttcaac
    12721 ctttttcaga ctgtcctgaa actcctggag ttttaactcc tggatcagtt cgttctgcac
    12781 cgttgtcgcc agtgaacgca acgcgcgaat ccagcccgct accgttttta ccgccacagg
    12841 cagtcgttgc ggccccagaa cgacgaggcc gatgatgaac accaatagca gttcgctaaa
    12901 accgatatca aacacggatt acacctgctc tttatcgtgg cgcttcgcgt cttctgtttt
    12961 agcctgttcc tgattcgtat ccgcctgctt atcggcgata gttttcacag taaaatcagc
    13021 atcctgactg gttttatcct gctttggttc atcatcgctc attgcttttt taaagccttt
    13081 gatcgacgca ccaagatcgg aaccgatgga gccgagcttt ttggtgccaa aaagcagtac
    13141 aacgatgacg gcaataatca ataactgcca aatactgata ccacccatac atgttcctct
    13201 gtggtagatg atgattaaac aaagccgcat tatacgttac acggcccgcc ttgagcgatg
    13261 aaaaaatcag cgtgttttgc gccaaccgac aaaccaggcg atcagaccac ctgccattaa
    13321 ccagccgggc atcagccccc actcaggtcg gctgaccaac aagaatgtgc cacttaatac
    13381 taacgtagcg ccaattccga gaaaataacg cgattgtccc tgacgtacat gatttgactg
    13441 aagctcgcgg gcaatcttat caacactgtg ctgtaaatac ttgccctggc gcaaactgtc
    13501 gtaaaccaat tcaggcagtt ctggcatttt ttcgacccag aacggcgctt tttctttaaa
    13561 tgctctcacc agcgcaggaa taccgacctg atctttaatc cacgactcca ggaaaggctt
    13621 cgctgttttc cataaatcga gctgcggata aagctggcgt cctacccctt cgacatagag
    13681 cagggttttc tggagtaaca ccagttgcgg ctgcacttcc atattgaagc gacgcgccgt
    13741 attaaacaga tttaacagta catgtccaaa cgaaatttcg gccagcggtt tctcaaagat
    13801 aggttcacag accgtacgaa tggcaaattc gaactcttca acgttggtat ctggtggcac
    13861 ccagcctgaa tcgacgtgta gctctgccac tttgcgatag tcgcgattaa agaaggcgat
    13921 aaagttttct gccagatagc gtttatcttc tttgtttagc gagccaacaa tcccgcaatc
    13981 aatgccgata tatttcgggt tttccgggtg ttcatagctt acgaagatgt tgccagggtg
    14041 catatcggca tggaaaaagc tgtcgcgaaa gacctgagtg aagaacacct gcacgccgcg
    14101 ttccgccagc aatttcatgt tagtgccgtt tttctccagc gccgcaacat cagacaccgg
    14161 aatgccgtaa atgcgctcca tcaccatcat cccttcacta caatagtcag ggtaaacttc
    14221 cgggatgtag agcatcgggc tgtcttcaaa attgcgccga agctgaatgg cgttggcaga
    14281 ttcccgcagc aaattcagtt catcaatcaa ggtcttttcg tactcgcgca ccacttcggt
    14341 tgggcgcaga cggcgaccat ccggcagcaa acgcggcacc cagcgagcca gacggtagat
    14401 aagtttcaga tccgctttaa tgaccggcaa aatatccggg cggatgactt taatcaccac
    14461 ctctttacca ttcgatttca atcgcgcggt atgaacctgg gcgatagaag cagaagccag
    14521 cggcttgatt tcaaaatcgt caaaccacgc ttctaccggc aagccgccca ttgcagcttc
    14581 aatctgctgc ttcgccagct tgccatcaaa cggagctact ttgtcctgca ataacgccag
    14641 ctgatcggca atatgcggtg gaaaaagatc gcggcgggtt gataacattt gcccgaactt
    14701 gatccaaacc ggccccagtt cttgcagggc cagtcgtagt cgctcaccta aaagtttgtc
    14761 tttatgccga tttggcatcc agaataatga gtatcgccat agccgtagcg gcagggtgat
    14821 acgcattttg gggatcagtt catcaagtcc gtagcttaaa aaagtgcgaa tgatgaaata
    14881 taggcgccgt acttcacctg gcgtcattta gcctccagtt tttccagccg tttggtcagg
    14941 gcatcaacag cacgctcgac ggcagccgtc tcttccgcaa accaggccac ttcaagcgga
    15001 ccgggtgcca tacgccactc ttcagtaatg gcttccgcca cataacgttg ctggcgctta
    15061 atgccgtgat gcaggaactt tgcgcctccg cgcatggctt tgctgattcc ttcagcggcg
    15121 atatcaccgg tataaggggc cagcagttcc gcagggtcga actctgccag atctgccagc
    15181 gcaacgaagt tttgcaccac ctgaatatcg ccctgcactt ccagctcacc actgcgaatc
    15241 agtgcggtaa gctgctggcg atcgcgaagt ttcggcaaca cactggcgta ggcgataacg
    15301 gtgcagtcag catcgcctgc ccattcgccc agtacatcaa cctggcgttc gctgaacacc
    15361 agaatcaatg acgtcgaaaa gccttttacc tccacgcgca atactttacc cagcagacgc
    15421 gagcgggctg ttttcagcgc gggtgagcga tacaggaagg tgttgagcag actttcaatt
    15481 cctgccgtca ctaaaggttt aaaaggcatt tccggtctcc tgtcagaact tataaccacg
    15541 atgcagcgcc acaacccctg ccgtcagatt gtagtagtcg acactttcga atccggcatc
    15601 ctgcatcatg gctttcaggg tatcctgatc gggatgcata cggatggatt ctgccagata
    15661 acggtagctg tcggcgtcgt tcgcgaccag tgagccaata cgcggcagca catggaagga
    15721 gtatgcatca taggctttgc tcagcggctc gataattggc ttcgagaact caagcaccag
    15781 caggcggccg ccgggtttca gcacgcgata cattgaacgc agtgctttat ctttgtcggt
    15841 gacgttacgc agaccaaacg aaatggtgat gcaatcaaag gtgttatccg ggaacggcag
    15901 cgcctcagcg ttcgcctgaa catactcaac gttgccaatc acaccgatat tacgcagctt
    15961 ctcgcgtccc attttgagca tggattcatt gatatcagca aggaccactt tgccagtttc
    16021 tccgaccagg cgggagaatt tcgctgtcag gtcgccggtg ccaccagcca gatccagcac
    16081 ggtctgccca cggcgtacgc cgctgcaatc aatcgtgaat cgcttccaca aacgatgaat
    16141 accaaatgac atcaaatcat tcatgacatc gtattttgat gccacggaat ggaaaacgtg
    16201 ggcgaccata tccgcttttt gttccttcgc gacggtctga aaaccaaagt gcgtcgtttc
    16261 ttgtgactta tccaccatct caatgcctgc tcatcaaaaa attgttccag aagtgtaaca
    16321 gattgggcgt cgatgcccta gatttctacc cggcttaact acccctaatg ggctagcgcg
    16381 actgctgatt atattcatca tcgcgttgat aagcttcatc attcggctgc tccggaaccg
    16441 accgaagtcg atactcttca tcctggctca ccgcctgttc agccaaatcc ggattaatct
    16501 cgcgtttaat ttctactcct aaaccgcgaa acgcttctgc ctgcgccagc acatttccgc
    16561 gacctgaaga gagttttttc attgcctggc ggtagttatc ctgcgctttg tcgagacttt
    16621 gaccaatcgc ggacatgtca tcgatgaaca aacgcatctt gtcgtacagc ttgctggcac
    16681 gatcggcgat ttgctgggcg ttgcggcttt gatgctcata acgccacagg ttggcaatag
    16741 tacgcaacgc caccagcagc gtagtcgggc taaccagcat gatattgttt ttcaacgctt
    16801 cggtgatcag ctccggctgg cggtcaagcg ccagtaaaaa agcgggttca acgggaataa
    16861 acatcagcac gtaatccaga gttcgcagcc ccggcagctg ttgataatct ttgcgtccca
    16921 gcaaacggat atggttacgc accgacgcga tatgttcctg tagcgcgctt tcgcgggtgt
    16981 agtcgtcttc ggcgttaaaa tagcgttcat aggcgaccag cgtcattttg gcgtcgatca
    17041 ccacatcttt tccctgcggc agccgcacga tgacatccgg ctgcatccgc gagcgggcgt
    17101 cattttcgat gctgacctgg gtttcatatt catacccttc acgcagaccg gaagcctcca
    17161 gcacccgcgt caatactacc tcgccccagt tgccctgggt tttattgtcg cctttcagcg
    17221 cgcgcgtcag gttgatcgct tcctgggcca tttgcgcgtt gagttgctgg agattgcgaa
    17281 tttcgtgggt cagggtatgg cgttcttgtg cttctttacc gaagctgtcc tgaacctgac
    17341 ggcggaaacc gtccagttgt tcacgtagcg gcgacaacag gctgttcaga ctctgacggt
    17401 tttgctcatc aacccggcga ttgctgtgct caaaaatacg gttggcgagg ttttcaaact
    17461 gctcactgag gcgctgctcg ctgttaatca tctggcgaat tttatcgtca gcatgttgct
    17521 gtgcggcttc catccgcgtg gttacttcac gcagatcggc ctccagagag gtgttaatac
    17581 tttgcaggct gcgcacttcg ttattgagta actcgcactc tgcacgccag tgctcgcttt
    17641 gggtaatttg ttgttttgcc gcgcttaact ccgcgaccat ctcttcacgt tcagctaatt
    17701 gctcggcttt ttgctgcgca tgttgataac tggcaaacag ccagccaatt gccacaccca
    17761 ccaacgcaat aactgcgtaa accatgattg agaaatccac aacgcctcct gacgcaacaa
    17821 cttacccgca caaaatagaa ttgtgctgta caaacgtcca gttggaaagg catttcctgc
    17881 gaggcgctac gcaaaaatac aaaaggccga acgcgtcggc cttcagacag gagaagagaa
    17941 ttacagcaga cgacgcgccg cttccaccac gattttcacc gcttggcttt cagtttgttt
    18001 catcgtctca gcattcggga tctcttgctg ggtgcggtta acgataacac ccgctaccat
    18061 accggcacgc aggccctgac ttgcacacat ggtcagcagg gttgcagatt ccatttcata
    18121 gttcattacg cccatcgcct gccactcttc catagaacct ttaaagtgac gaactacgcg
    18181 accagagtaa gtatcgtaac gttcctggcc tgggtagaag gtatcagaag aagctgtcac
    18241 gccaacgtga gttgtcgcgc caatggattt cgcagcttca accagcgcag tcgtacattc
    18301 gaaatcagcg acagccggga attccagcgg tgcgaagtgc aggctcgcgc catccagacg
    18361 gacagacgcc gtggtaacca ggacatcacc cacattaata tgcggctgaa tagcgcccgt
    18421 tgtaccgata cgcaggaagg tgcgaatgcc cagctgtgcc agctcttcaa cagcaataga
    18481 ggtagacggg ccgccgatac cggtagagca gacgataaca ggtttaccat ccagctctgc
    18541 acgccaggtg gtgaattcgc ggtgagatgc cagcttaacc ggcttatcca tcagcgcggc
    18601 gatcttttcc acacgatccg ggtcgccagg gacgatggca agcgtagccc cttgtaaatc
    18661 gtttttagtg aggccgagat gaaaaacatc agacttggac atatacaact cctctgtgaa
    18721 tcggtttagt cagaagaagc aaaaagacac tttaccgaag ggtttaacat tttttcgtga
    18781 tactcatcac catgacgcaa atgcgttgca taaatttacc caccaaaagt gatgcaaatc
    18841 acataaatca ctcttcattc tgcaggactg cataaaagat gagccgattc aggcaatgtg
    18901 atttgttgca tgctgactat agttaacatt gcgcaacatt tttataaggg tacggagaat
    18961 accatggcaa caacacaaca atctggattt gcacctgctg catcgcctct cgcttcgacc
    19021 atcgttcaga ccccggacga cgcgattgtg gcgggcttca cctctatccc ttcacaaggg
    19081 gataacatgc ctgcttacca tgccagacca aagcaaagcg atggcccact gccagtggtc
    19141 attgtagtgc aggaaatttt tggcatgcat gaacatatcc gcgacatttg tcgccgtctg
    19201 gcgctggagg ggtatctggc tatcgcacct gaactttact tccgcgaagg cgatccgaat
    19261 gattttgccg atatccccac gttgcttagc ggtctggtag caaaagtgcc tgactcgcag
    19321 gtgctggccg atctcgatca tgtcgccagt tgggcttccc gcaacggcgg cgatgttcat
    19381 cgtttaatga tcaccggatt ctgctggggt ggacgtatca cctggctgta tgccgcgcat
    19441 aatccacagc taaaagctgc agtggcgtgg tacggcaaac tgacgggcga caagtcgcta
    19501 aattcaccga aacaacctgt tgatatcgca accgatctta acgcgccggt tctcggctta
    19561 tatggtggtc aggataacag cattccgcag gagagcgttg aaaccatgcg ccaggcgctg
    19621 cgggctgcta atgcgaaagc agagattatc gtgtacccgg acgccgggca tgcattcaac
    19681 gctgattatc gcccgagcta tcatgccgaa tctgcgaaag atggctggca gcgtatgctg
    19741 gaatggttta agcagtatgg tgggaagcgg agttagtttc ctcttagtct atatttgaga
    19801 tggtgagtga acgagtgcgt tttgctgcca tctctgtccc ccaaaaaata atcagtatga
    19861 attgattgga gattacgact ccgctatttg accttaagaa ggtcggcaag tcatagtcat
    19921 aaatgagtct cttcaatgga agacacctta gagatttacc atctactccc attcacaaac
    19981 aaacattaat tattatcaac acattcacat taattataaa tcatctcaga ccttcctgat
    20041 aaaaagatat taaagtgaaa cattttgttt ttcacatttt agttcatcac ttttaggcgt
    20101 aaataaaggt aaaaaaaaca acaatactat ttctctttca caaaagaatt aacaaaaggt
    20161 tttttaagga tgagtgatat tatttacttg accataaccg gagaacaaca aggatgtatt
    20221 tcctctcttt gtggaacgag cgcatcaata ggtaatcgct ggcagattgg ccatgaagat
    20281 gaaatcttcg cgttctcgct ctctaatagt attacgaatg caggcaaagg gtcacaattg
    20341 catggcctta gtttctgtaa attcttatta acgctattaa caataacgag cagttgttca
    20401 tggcgtttga cttctatcgg atcaatagat ttggtcgatg ggagaaatat tacaacattc
    20461 aactccgcgg cgcgctctta tcagcaatca atcacctgtt tactgagaac aatctggata
    20521 cagaagccat cactgtcagt tatgaatata tactctccag gcatctgatt gctaatactg
    20581 aatttagtta tttggcattt cccgataact acaaccgtct gtttatacca cggccaaaaa
    20641 ctaaagctgg taatggttca aaacacttaa tagtaaagcg gttggcagac tgcttgctgc
    20701 tggcggcata tacaatggga atatcgaagg attcagggaa acagcagaca agttgggggg
    20761 agatgcacct gctggttacg atcaatttat ggataacaaa gggttactca tcgctggtgc
    20821 ttcaatagtt gctggagcag ttctggggag aatgagactc cctgatttgg aatcacttga
    20881 gcatttcgga gccagaggag ca
//
LOCUS       DAJVTY010000060        20694 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVTY010000060 DAJVTY010000000
VERSION     DAJVTY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.060"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..224)
                     /gene="hyfA"
                     /locus_tag="OQQ66_002002"
     CDS             complement(<1..224)
                     /gene="hyfA"
                     /locus_tag="OQQ66_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1904944.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDA"
     gene            complement(477..947)
                     /gene="bcp"
                     /locus_tag="OQQ66_002003"
     CDS             complement(477..947)
                     /gene="bcp"
                     /locus_tag="OQQ66_002003"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1904945.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(947..1519)
                     /locus_tag="OQQ66_002004"
     CDS             complement(947..1519)
                     /locus_tag="OQQ66_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1904946.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1665..2543
                     /gene="dapA"
                     /locus_tag="OQQ66_002005"
     CDS             1665..2543
                     /gene="dapA"
                     /locus_tag="OQQ66_002005"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1904947.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2560..3594
                     /gene="bamC"
                     /locus_tag="OQQ66_002006"
     CDS             2560..3594
                     /gene="bamC"
                     /locus_tag="OQQ66_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1904948.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3807..4520
                     /gene="purC"
                     /locus_tag="OQQ66_002007"
     CDS             3807..4520
                     /gene="purC"
                     /locus_tag="OQQ66_002007"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1904949.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4688..5551
                     /locus_tag="OQQ66_002008"
     CDS             4688..5551
                     /locus_tag="OQQ66_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1904950.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5566..7581
                     /gene="tmcA"
                     /locus_tag="OQQ66_002009"
     CDS             5566..7581
                     /gene="tmcA"
                     /locus_tag="OQQ66_002009"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1904951.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7655..8353
                     /gene="ypfH"
                     /locus_tag="OQQ66_002010"
     CDS             7655..8353
                     /gene="ypfH"
                     /locus_tag="OQQ66_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1904952.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8463..8663)
                     /locus_tag="OQQ66_002011"
     CDS             complement(8463..8663)
                     /locus_tag="OQQ66_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1904953.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8691..9818)
                     /gene="dapE"
                     /locus_tag="OQQ66_002012"
     CDS             complement(8691..9818)
                     /gene="dapE"
                     /locus_tag="OQQ66_002012"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1904954.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9822..10178)
                     /locus_tag="OQQ66_002013"
     CDS             complement(9822..10178)
                     /locus_tag="OQQ66_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1904955.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10559..10618
                     /gene="ypfM"
                     /locus_tag="OQQ66_002014"
     CDS             10559..10618
                     /gene="ypfM"
                     /locus_tag="OQQ66_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1904956.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10717..13825)
                     /gene="acrD"
                     /locus_tag="OQQ66_002015"
                     /pseudo
     CDS             complement(10717..13825)
                     /gene="acrD"
                     /locus_tag="OQQ66_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(13988..15688)
                     /gene="narQ"
                     /locus_tag="OQQ66_002016"
     CDS             complement(13988..15688)
                     /gene="narQ"
                     /locus_tag="OQQ66_002016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS1904957.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15894..17873
                     /gene="aegA"
                     /locus_tag="OQQ66_002017"
     CDS             15894..17873
                     /gene="aegA"
                     /locus_tag="OQQ66_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1904958.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAIRHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17941..18516
                     /gene="nudK"
                     /locus_tag="OQQ66_002018"
     CDS             17941..18516
                     /gene="nudK"
                     /locus_tag="OQQ66_002018"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1904959.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18642..19685
                     /locus_tag="OQQ66_002019"
     CDS             18642..19685
                     /locus_tag="OQQ66_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1904960.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19795..20694)
                     /locus_tag="OQQ66_002020"
                     /pseudo
     CDS             complement(19795..20694)
                     /locus_tag="OQQ66_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         4999 a         5291 c         5540 g         4864 t
ORIGIN      
        1 atcgcatcat ccctctggga gatggcattg accgggcaga cctgcaggca aggggcttcc
       61 tcacagtgat gacacacgac aggggcagtg attgttgacg tcttcgccag ggccaggcgc
      121 gggtgttgct gtaaaccttg cgttttatgc acgtccgaac aggcagcgag acaggtatta
      181 catcctgtac accacagtgg ttcggccacc acaaagcggt tcatgtcggc tccttgaaaa
      241 tgatatgaaa cattgcattt catattgcat aaaggatgcc agtctgatta atttaatata
      301 ttcaataaat tatgtattaa cagggttctg aacggtacaa aaatgatggt cattaacgtt
      361 cagcaaaaaa tggattcgac acccgtgtcg caggggaaag cgagaatgtg atcttgatca
      421 cgagaagtga ggtgtgctga ccgcaagcgc agccagcacg gaatggagca aagtaatcag
      481 gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat tgctggtttt aaaatcgtca
      541 aagacatgtt cgattttgcc atcagcgtca atcaggaagc tgatgcgatg aataccatcg
      601 taggttttgc ccatgaagga cttttcaccc cagacgccga attgttcgca cacctggtgg
      661 tcctcatcag acaggagcgt aaagttaagc agctcttttt ccgcaaaacg ggagagtttt
      721 tcgggtttat cggtgctgat acccagcaca tcaacgcccg cttttttcaa ctcatccatg
      781 ttatcgcgta agccgcaggc ctgtacggta cagccggggg tcatggcttt cgggtagaaa
      841 taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc
      901 ggcaagctaa atttcggtgc gatatcaccg gctttcagtg gattcattac ttaactccat
      961 cctgttcatc atgttgggaa taattgacga cgttaatact gccttgtgca ttgagttctg
     1021 tacatagggc tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg
     1081 cggtgatctg aatatgcaac tgcgcagccc gttcattttc agcaggttgc gtgcgcgaca
     1141 ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat
     1201 gcggggagtc tgcaacatcg acctgaaccc agacagatgc tggcattggc ggacgcggac
     1261 gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc ggcacctttc aacggtaacg
     1321 ttgattcaat cagagtaatg gcattccatg aaccggaaag cagcatgata aacgtgaact
     1381 cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta ctgacatgac
     1441 gggtgatggt gttcacaatt ccagggcgat cggcacccaa cgcagtgatc accagataat
     1501 gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc ttaaggaaag
     1561 cataaaaaaa acatgcatac aacaatcaga acggttctgt ctgcttgctt ttaatgccat
     1621 accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc acgggaagta
     1681 ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa
     1741 aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt ggcaccactg
     1801 gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg acgctggagc
     1861 tggctgacgg gcgcattccg gtgattgccg ggaccggtgc taacgctact gcggaagcca
     1921 ttagcctgac gcagcgcttc aatgacagcg gtatcgtcgg ctgcctgacg gtaacccctt
     1981 actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc gctgaacata
     2041 ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg
     2101 aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag gcaacaggga
     2161 acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt ctgctgagcg
     2221 gcgatgatgc gagcgcgctg gacttcatgc aattaggcgg tcatggggtt atttccgtta
     2281 cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca gaagggcatt
     2341 ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg
     2401 aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg gcgaccgata
     2461 cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc agagcggcgc
     2521 ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc tgttcaaaag
     2581 tcgcgcctgg caaaggttgc gggtgtttcg cttattttat tactcgctgc ctgtagttct
     2641 gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc ggcaccgctt
     2701 gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga ttatgcaatc
     2761 ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc accagcccag
     2821 ccgctggcac tggtttctgg cgtgcgtacc cagttcacgg gcgataccgc ttcattgctg
     2881 gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa
     2941 aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccatcga ttgggtacaa
     3001 tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg
     3061 cagggttatc agcaggcggt tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg
     3121 gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt tatttccgcc
     3181 ggtctggata aatctgcctc tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact
     3241 atggacgtac aaagtgcagc tgatgacacc ggtttaccga tgctggtcgt acgcgggccg
     3301 ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat gaaagtgacc
     3361 gacagcaccc gttcgcaggg taacatggcc gtaacttata agccgctgtc tgacagcgac
     3421 tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa attgcaggtt
     3481 ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca tactctgact
     3541 cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatat
     3601 agggctggag tcatccggcc cttttttctg atatgatacg caaacgtgtg cgtcggcaga
     3661 aaaatgcaat tttagcggta attcgcacga aatttgtttg tcggacgtag gtcggataag
     3721 gcgttcttgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg
     3781 ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag
     3841 cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc cgcaatgata
     3901 cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg caaaggtatg gtgaacaaca
     3961 agttcaacta cttcattatg agcaaactgg ctgaagcggg tatcccgact caaatggagc
     4021 gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg gttgagtgtg
     4081 tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg
     4141 agctgaaccc gccgctgttc gatctgttcc tgaaaaacga tgccatgcac gatccgatgg
     4201 tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg gcgcgtatga
     4261 aagagctgac ctacaaagcg aacgacgtgc tgaaaaaacg gttcgatgat gctggtctga
     4321 ttctggtcga cttcaagctg gagtttggtc tgtacaaagg tgaagtggta ctgggcgatg
     4381 agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa atggacaaag
     4441 accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc gcccgccgcc
     4501 tgggtgtaca gctggactga tttttctgtt catcatcttg ccgtgcagct ggcacggcaa
     4561 gacaaccgct ctcggaatat atgcattgaa cgctcattcc tccgccattt catcccgctt
     4621 tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat atattgttga
     4681 ggtatctatg cgttggcaag ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag
     4741 ctccggtggt ccgtctatgg gcggtcccgg ttttcgcctg ccaagcggta aaggcgggct
     4801 gattttactg atagtcgtgc tggttgcagg ctactatggt gttgatttaa ccgggttaat
     4861 gactggacag ccggtttccc aacaacaatc aacgcggtcc attagcccta atgaagacga
     4921 agctgcaaaa ttcacctcgg tgattctggc aaccacggaa gacacctggg gacaacagtt
     4981 cgagaagatg ggtaagacct atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg
     5041 caccggctgc ggggcgggcc agtccattat ggggccgttc tattgcccgg cggatggcac
     5101 agtttatatc gatctctcct tctatgatga catgaaagac aaacttggcg cggatggcga
     5161 ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga aactgttagg
     5221 catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag tgaaccgctt
     5281 atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc ataacatgca
     5341 gcagcaaggc gttctggaaa ccggcgatct ggaagaggcg ctgaatgcgg cgcaggccat
     5401 cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca gtttcacaca
     5461 tggcacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg gcgatccggc
     5521 acaatgcaat acttttggta aaagcattta atttccgggg cagggatggc tgaactgact
     5581 gcgctttaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg
     5641 agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc cttacttggc
     5701 gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc ctcggcgcta
     5761 caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca cggctttgat
     5821 gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct ggttttgtta
     5881 ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat
     5941 tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt acttacggcg
     6001 gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca ttttactccc
     6061 cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca actcttacag
     6121 cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg gcgcggtaag
     6181 tcggcgctgg cagggcaact catttctcgt attgcgggca gtgcgattgt caccgcgccc
     6241 gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg ctttattgcg
     6301 ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga tgaagccgca
     6361 gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac gttgttaacc
     6421 actacggtgc agggctacga aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc
     6481 tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca gggatgcccg
     6541 ctggaaaaaa tggttagtga ggcactggtt tttgacgatg aaaacttcac ccatacacca
     6601 caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga gccagaaacg
     6661 ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacttcgcc gctggattta
     6721 cgccggatga tggatgcacc agggcaatat tttttacagg cggctggcga aaacgagatt
     6781 gccggagcgc tgtggctggt ggatgagggg ggattatctc aacaactcag tcaggcggta
     6841 tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc ggcgcacggt
     6901 agcaatccac tggcagcgac attgcgtgga cggcgggtca gccggatagc agttcatccg
     6961 gctcgtcagc gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca atatacgcaa
     7021 gacctcgact atctttcggt gagttttggt tacaccgggg agttatggcg tttctggcaa
     7081 cgctgcggtt ttgtgctggt acgaatgggt aatcatcgtg aagccagcag tggttgctat
     7141 acggcgatgg cgctgttacc gatgagtgat gcgggtaaac agctggctga acgtgagcat
     7201 tactgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac acttcctgtt
     7261 gatccactaa acgatatcgt gctttctgac gacgactggc ttgaactggc cggttttgct
     7321 tttgctcatc gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct acaaaccagt
     7381 gaactggcat taccggcgct gcgtgggcgt ttacagaaaa atgccagtga tgcgcagtta
     7441 tgtaccacac ttaaacttgc aggccgcaag atgttactgg tccgtcagcg ggaagaggcc
     7501 gcgcatgcgc tgttcgcact taatgatgtt cgcactgagc gtctgcgcga tcgcataacg
     7561 caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt gagccagcgg
     7621 cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt ttgttgttca
     7681 aagcccggat aaacctgtgc aacagttgtt actgcttttc ctcggtgtcg gggataactc
     7741 ggtggcaatg ggggaaatag gcagttggtt tgcaccgctg tttcctgatg cgctggtggt
     7801 gagtgtcggt ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca
     7861 gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga cgtttattga
     7921 aacggtacgc tactggcaga aacagagtgg agtaggggct aacgccacgg cgttaatcgg
     7981 tttctctcaa ggcgcgatta tggcgctgga gagtattaaa gccgaaccgg gccttgcttc
     8041 acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaaccgcgt ctacagcaac
     8101 gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc atgcggttgc
     8161 ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg tggaggattt
     8221 aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc gttataccat
     8281 tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg atgatgtgat
     8341 tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt cgcatcaggc
     8401 atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt tgagcactca
     8461 gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca cttatcgttg
     8521 aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttgtg gtcgacgcgc
     8581 ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac cagaatccac
     8641 caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca tcaggcgacg
     8701 agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc agcgttcaca
     8761 cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac ctgcgccccc
     8821 atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg cggtttaatt
     8881 tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg cgcggtcaaa
     8941 aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt ttcaagcagg
     9001 gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg gaagttaaac
     9061 tgcacaaaca gttcacccgg aataacattg ttactgcccg ttcccgcctg aatattagca
     9121 atctgcatac tggtcgccgg gaaaaattca ttgccctgat cccactcaat agccactaat
     9181 tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgcgg gtaggccaca
     9241 tgcccctgaa cgccatgaat ggtaaggttg caggttaacg atccgcgacg accatttttc
     9301 accacatcac caaccacttc gatactcgac ggttcgccga ccaggcagta atcgagacgc
     9361 tcattacgtg ccatcaacgc ttcgacgact tttaccgtac cgttgtgggc actggcttct
     9421 tcatcagagg tgatcagaaa tgccagtcgc cccgtatggt tgggatgttg tgcgacaaag
     9481 cgttctgccg ctaccaccat cgccgccagc gagcctttca tatctgccgc accgcgcccg
     9541 aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg atcggcgtcg
     9601 ccaggcggca ccacgtcggt atgcccggca aaggctaacg tttcaccttg cccacgccat
     9661 gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc gatcgcctgc
     9721 aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga agggcggcga
     9781 ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc atggaaaaat
     9841 tgctgataac tggaatcact gaaacccagc agcataggct taccaggcgc gcagagcaat
     9901 ggacgtttga taattgaagg catttcagtc attaatgccg ccgcagaggc cgcatcggtg
     9961 attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt gttcagtaac
    10021 gcttcccagc ctaattcgtt gataaaaccg ttcaataatt cgctgtccag cccatcgacg
    10081 cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg agccttttta
    10141 atggtgtcac aatttttgat gccgtaaagt gtaaccataa tgaatccttt ttcgatctat
    10201 gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta tcaggaaata
    10261 taacattatc gaagtattca aattaacaac tattaaataa tattaaacat ttttaacgta
    10321 aacattaaga atattcatct tgttatctgt ctgtatttta accagaatta aatcagtatt
    10381 agatgccgta tgaatatctc gaaatgttgc tgtgtatcac atacaaatta tcaccgcagg
    10441 accatagtat ttacataagg tttgtccccc ggaggtagtc atcgcagcaa tcgagtaaat
    10501 tagcttcacc acggtatatt gtttccgtag aatacccata attataagag aggttgttat
    10561 gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc gtaagtaatt
    10621 cctgagacaa agtacaaaca gcaagaaccc gctgagcagg ttcttaatcg gaaagttgaa
    10681 aaaggcgaca cattagcatg tcgccttttt tattgcttat tccgggcgcg gcttcagcgg
    10741 gaagcggcgg cgcaccagta caaagaacag cggcacgaag taaatagcca gaatagtggc
    10801 cgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg aaccggcacc
    10861 cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca tcagaatcgg
    10921 gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc ctttttggtt
    10981 catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac caatgaccgt
    11041 taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg ttgccagcag
    11101 cgcgccgata acccccagcg gcacgaccag cattaccgag aacggcaccg accagctttc
    11161 aacgccgcca gacacaggaa taccaccagc aaggaaatgg cgtacagcgc cggagcctgc
    11221 gcgccggaaa gccgctcctg atacgacatc gccgtccact ccagaccaaa gccgtttggc
    11281 aactgcttca ctaacgattc cattatatcc atcgccgtac cggtactgac ccccggtgcg
    11341 gcttccccaa caatctcaac cgcagaataa ccgttatagc gttccagacg cggcgagcca
    11401 gtctcccagc gtgaggtcgc gaaagcagag aagggcacca tgccgccatc tttatttcgg
    11461 acataccaga gattaatgtc atctggcagc atgcgatacg gcgcagctgc ctgcacatag
    11521 actttcttca cacgaccgcg atccataaag tcattcacat agctcgaacc ccaggcggtt
    11581 tgcagtgtgt cgttaatatc gtcgatagca acgcccagcg cctgagcttt acgctggtcg
    11641 atatcaatct gcaactgcgg actgtcatcg aggccgttat ggcgcacacg ggttagctcc
    11701 gggttttccg ccgccagcgc cagcaactgg ttacgtgctg ccatcagcgc atcgtgacct
    11761 gctccagcgt ggtcctgcaa ctccatatca aaacccgcag aactaccaag accgctaatt
    11821 gctggcgggc tgctggcgat aacgcgagct tctttaatct tgttaaacgc ctttgttgca
    11881 cgctcgataa tggcaaacga ggtgccggtc ttactgtcgc gttcgctcca gtctttcagg
    11941 cggataaaca ttcgcgccac gttttgcccg ttacccccag ggccagaacc aacggtggca
    12001 aacaccgaca tgatgttgtc tttttcatgg gtgaagtaat atttctcgat ttgctcaacg
    12061 actttcaggg tctgttgttg ggttgaaccg ctgggcaatt gtaccgaggt agtaaacatg
    12121 ccacggtctt ccagcggtaa gaacgacgtc ggcaaacgca ggaacaggaa caccatgccg
    12181 ccaagcagca ggacataaat cacaatccag cgcaggctgc ggtggagaat tttcgccacc
    12241 cctttttcgt agcgttcggc gttgcggtta aacatctggt taaaccaggc aaaaaagcct
    12301 ttttgcccgt gatgctcacc ttttttcagt ggcttaagta gtgtggcaca cagagccgga
    12361 gtgaggatca tcgctaccag tactgacagc accatcgccg caacaatggt aatagagaac
    12421 tggcgataga tggcaccggt ggtgccgccg aagaaggcca ttggtacaaa taccgccgaa
    12481 agcaccatcg caatcccgac cagtgccccc tggatctgcc ccatcgattt acgtgtggct
    12541 tcgcgaggag tgagtccttc ctcactcata atacgttcga cgttttccac caccacgatt
    12601 gcgtcatcca ccagcagacc gatcgccagt accatcgcga acatggttaa ggtgttgacg
    12661 ctgtaaccga aggcgtaaag tacggagaag gttcccatca acaccaccgg cacggcgata
    12721 gtgggtatca gcgtggcgcg gaagttttgc aggaacagat acataacgag gaaaaccagg
    12781 gcgatagctt ccagcagcgt tttcaccacg tcttcaatgg aggctttaac aaacgaggtt
    12841 gtttcatacg ccaccttgta ttccagtccg tgcgggaagt actgcgccag ctcgtcgaga
    12901 cgattgagca ccagctccct gtcgccattt cgtttgcgcc ggaggccagt tttaccccca
    12961 gcccggaggc tggcttacca ttgaagcggc taagataatc gtatttctcc gcccccattt
    13021 cgacggtggc gacatcgccc agccttacct ctgagccgtc ttgattcacc cgcaaggtga
    13081 tatcgcggaa ctgttctggt gtttgcagca gtgactgggc gttaatggtg gcgttgagcg
    13141 cctgcttatc gacggaaggt gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg
    13201 actcaatggc atcagtgaca tctttagccg tcatctggaa actgttgagt ttcgccgggg
    13261 ccagccagat acgcatggaa tattgcgaac cataggcatc gatatccccg acgccattca
    13321 cgcggcttaa cgggtcctga atattactgg caacataatc ggcaatatcc tgtttatcca
    13381 tcgaaccatc ggtagagacg aaagcaatgg tcagaatatt ggtatcgccg gttttacgca
    13441 ccgtcacgcc ctgattttgc accgcctgcg gtaactttcg catggctgat tgcagctggt
    13501 tttgtacttg ctgcacggct tcgtccggat cggtgcctgc tttaaaactt aaagtgacag
    13561 atgcctgacc ggtgccactg ctctgagatg acatatacat gagattatcg aggccggtca
    13621 tattttgctc gataacctgg ggcacggtgt tttccagcgt ctgggccgat gcgccgggat
    13681 agttagcggt cactcgcaca ttcggtggcg cgagatcggg gtattgttca acgggcaatg
    13741 aaaaaatcgc tagggtccct gtcagacaca acaggattgc cagcacccag gcaaaaatgg
    13801 ggcgatcaat aaagaaattc gccattaaaa gaggacctcg tgtttctgcg tatcgttgta
    13861 ggcttgactt gcttcactgt agcggcaagg tacgagccaa tcgtggagaa aaaaggagtt
    13921 aatgtaaatt atcatgccct tttatactga accttaagtg caagtattct ttggtcagta
    13981 ggaggcatta catcaactga ctttcctcac cctctgcaga gcgaaagcta atactcacta
    14041 acgtgccgcc gccggaaggt tgtgaaaaag tcagcgtccc acccagccgt tccgcgcgtt
    14101 cgcgcatgat attcagacca taatgacctt cgggttcttt cggttcaccg ataccaatcc
    14161 cgttatcacg gatataaacc gtgtgattgc cgtccggcgc ggtgacgcaa ctgacggcga
    14221 tttcgctggc gttggcgtgc ttcatcgcat tcagcaccgc ttcgcgaata atttgcaaca
    14281 aatgcacctg catttgcgca tccagcgcaa gggttggcag acggcagtcg agggtcagtt
    14341 tggcgctggt ttgattttgt aacgtatcca gcatttccct caacgcggag gggagatccg
    14401 cctgctgcag cgtcaggcgg aaagtagtca acagctcgcg taactgccga taagcatcat
    14461 tcaacgcctg ggaaaaatcg gccatgatac tttgtgcggt ggcgttatct tccggtatcg
    14521 aacgcttcag taacgtcaac tggatacgta agtaagaaag tacctgagcc agcgagtcgt
    14581 gcaattcgcg ggcgatggtt gcacgttctt ccatcaacaa taaatgctga aaatgcttct
    14641 gcgcctgatt aaagtacaaa ccgcgtccca gcatcgacga aacgctgtta agcagcggtt
    14701 ctgatgatga aacgtgacta ttttgccagt gcagttcgcc gtaaaccgtc tcttgcattg
    14761 tcaccggtaa aatctgcatc ggcaattccg ggtttggttg cccttcgcta atccgccagt
    14821 tttcaccgac atttaactcc agatattcag ccgcttcatt gtcgcgaaca atctgcaaaa
    14881 tatggcggaa acaatgcaca tcaatctggc ttgtgttcag cgcctgcgaa cattgataca
    14941 acacctccag acgacgcttg gcctcgtgga gatcgcgggt cttttcttct actgacgcct
    15001 ccagcgaaag gtacaattta tgcagctcgc tcgacatctg gttaaaggtt tttgcaagca
    15061 gaccaagctc attcggcaga ctggtatcca gcggcggcga gtcgaactgc ccgtgttcaa
    15121 tacgttgact ggcggtaacc agctgattca gcggggcaac cacctgatgg cgtatacggc
    15181 gcaaagtaaa aaagaccagc gtgaaaatac cgataccgcc agccagggaa atcgccacca
    15241 ccagcagcat tttgcgttca gcgtagtgct gtaaagccag cacgaacagg tctatctgat
    15301 taacataatt attaatattg gcctgatacc acggcaaatc gcccttgctg agccgattat
    15361 tcatctccag ccagttggca ttcagatgcg catagcgagt ttttactgct tctggcacat
    15421 accagacgtt gagattggtt aataccggtg aatgcagtgc ctgctgaaac aactggcgat
    15481 gtgcattgag ttgtggactg ccactttgca agtcgtagcc caggcggtaa ctctgcatac
    15541 gcagcgatcc ggcaatattg atagcctcag cgtcgcgcaa actgctcgcc agagttagca
    15601 gagcgatacc cgtggaaaga atcgacagca gcacaatgta aaaaaaggcc cgggccagac
    15661 tggccgagac gggtcgttta acaatcacac gcgtcttctc cacaaaaaat cattaatgtt
    15721 ccagttcgct gaaacgcaat ttagtcagtg atgggattat ttgagctgcc acagtttata
    15781 gcgaaatagt tacttattct gggcacatga acattgccga ttcccctgta agtcgggtaa
    15841 taacaacact catataaaga ataaggtttt tacaaccaaa aaagaaggtc gttatgaatc
    15901 gttttattat ggccaacagt cagcaatgtc tgggttgtca tgcttgtgaa atcgcctgtg
    15961 tcatggctca caatgatgag caacatgtcc tgagccaaca ccattttcat ccccgaatta
    16021 cggttatcaa acatcagcag caacgtagtg cagtgacctg tcaccattgt gaagatgcgc
    16081 cctgcgcccg tagctgccct aatggcgcaa tccgccacgt tgatgacagc attcaggtca
    16141 atcagcaaaa gtgtattggc tgtaaatcct gcgtggtggc ctgtccgttt ggtacgatgc
    16201 aaatcgtcct gacacccgtc gcggcaggaa aagtaaaagc cacggcgcat aaatgcgacc
    16261 tttgtgcagg gcgcgaaaac ggtcctgcct gtgtcgagaa ttgcccggcg gacgcgctgc
    16321 aactggtcac tgacgtcgca ctctccggca tggcgaaatc ccgccgcttg cgcaccgcac
    16381 gtcaggagca tcaaccgtgg catgccagta ccgctgcaca agaaatgccg gtaatgagta
    16441 aagtcgaaca aatgcaggca acgcccgcgc gtggcgagcc ggataaactg gcgattgaag
    16501 cgcgcaaaac cggttttgat gaaatttatc tgccatttcg cgccgaccag gcacaacggg
    16561 aagcctcgcg ctgccttaag tgcggcgagc acagcgtttg tgaatggacc tgcccgctgc
    16621 ataaccatat tccgcagtgg attgaactgg tgaaagccgg aaacatcgac gccgccgtcg
    16681 agctttctca ccagaccaac accctgccgg aaattaccgg acgcgtttgt ccgcaagacc
    16741 gtttgtgtga aggtgcctgt actattcgcg atgagcacgg cgcggtaact atcggcaaca
    16801 ttgaacgcta tatttcagat caggcgttgg cgaaaggttg gcgtcctgac ttaagtcatg
    16861 tcaccaaagt ggacaagcgg gtggcgatta tcggggcagg tccggcaggg ctggcctgtg
    16921 cggatgttct gacccgcaat ggcgtggcgg tgacggtgta cgatcgccat ccagaaatcg
    16981 gtggcttgct cactttcggc attccttctt tcaaactgga taaatccctg ctggcacgcc
    17041 gtcgggaaat cttcagcgcg atggggattc acttcgaact caattgtgaa gtgggtaaag
    17101 atgtctcttt ggattcgctt ttggaacaat acgacgcggt cttcgttggc gtaggcactt
    17161 accgttccat gaaagcgggt ttacccaatg aagatgcgcc gggcgtttat gacgcgctgc
    17221 cgttcctcat cgccaacact aaacaggtga tggggctcga agagctaccg gaagagccgt
    17281 ttatcaatac cgccggactt aacgtcgtgg tactgggcgg cggcgacacc gcgatggact
    17341 gtgtgcgtac tgcgctgcgc cacggcgcga gtaacgtcac ctgcgcttat cgtcgtgatg
    17401 aagctaacat gccaggctcg aagaaagagg tgaagaacgc ccgcgaagag ggggccaact
    17461 tcgaatttaa cgtccagccg gtggcgcttg agctgaatga acaaggtcac gtttgcggga
    17521 ttcgtttcct gcgcacgcgt cttggcgagc cggatgccca ggggcgtcgg cgtccagtac
    17581 cggtggaagg cagtgaattt gtcatgccag ccgacgcggt gattatggcg tttggcttca
    17641 atccgcacgg gatgccgtgg ctggagtcgc acggtgtaac ggtagacaaa tggggccgca
    17701 tcatcgcgga tgtggaaagc cagtaccgtt accagaccac caatccgaaa atcttcgccg
    17761 gtggcgacgc cgtgcgtggt gcggatctgg tggttaccgc aatggcagaa ggacgtcatg
    17821 cggcacaggg gattattgac tggctggggg taaaatcagt caaatctcac tgatagcctg
    17881 cgcagacaaa cccgacttca cagcgtaaga taattgttca tttcgcgctg tggagtcggt
    17941 atgacgcaac aaatcaccct cattaaagac aaaattctct ccgataacta tttcaccctg
    18001 cacaacatta cctacgatct cacgcgcaaa gatggcgaag ttatccgcca taaacgtgaa
    18061 gtttacgatc gcggtaatgg tgcgacgatc ctcctgtaca acgcgaagaa aaagaccgtg
    18121 gttctgattc gtcagttccg tgtcgctacc tgggttaatg gcaatgaaag cgggcagctg
    18181 attgaaacct gcgccgggct gctggataac gacgaaccgg aagtgtgcat tcgtaaagaa
    18241 gcgattgaag agacgggcta tgaagtgggc gaagtgcgca aattatttga actgtatatg
    18301 tcgccaggcg gtgtgactga gctaatccac ttttttatcg ccgaatacag tgacaatcag
    18361 cgcgctaacg ccggtggcgg tgtcgaagat gaagatattg aagtgctcga gctgccgttc
    18421 agccaggcgc tggagatgat caaaaccggc gagatacgtg acggtaagac ggtgttattg
    18481 cttaactatt tgcaaacgtc acatttaatg gactgaaaaa taacaataat atttcgttgt
    18541 ttattattgg ctcaatccga caaatccgat tgagccgcgc tacctgcgca acgaagatac
    18601 gacttgtgct gtttgtttga acttctgggg tcgtaccgtc catgcgctat cgcattttcc
    18661 ttctcttttt tttcgctttg ttgccgacgt ctttggtgtg ggcggcacca gcgcaacggg
    18721 cgttttccga ctggcaggtc acctgtaata accaaaactt ctgcgtggcg cgtaatacgg
    18781 gcgatcataa tggactggtg atgaccctga gccgcagcgc cggggcgcat accgatgccg
    18841 ttttacgtat tgagcgcggc ggattgaagt cgccggaggc gtcagaaggg gagatagcgc
    18901 cacggctgct gttagatggc gagccgttgg cgttaagtgg tgacaagtgg cggatttcac
    18961 catggttatt agtaaccgat gatacggcaa ccatcaccgc gtttttgcag atgattcagg
    19021 aagggaaggc gatcacctta cgcgacggca atcagaccat ttcgctgagt ggcttaaaag
    19081 cggcgttgtt gtttattgat gctcagcaaa agcgcgttgg cagtgaaacc gcgtggatca
    19141 agaaagggga cgaaccgccg ctcagcgtac cgcccgcgcc tgcgctgaaa gaggtcgcgg
    19201 tggttaaccc aacgccgacg ccactctcac tcgaagaacg caacgatttg ctggattatg
    19261 gaaactggcg gatgaatggt ctgcgctgct cgcttgatcc attgcgtcgt gaggtgaatg
    19321 tcactgcgct gactgatgat aaagcgctga tgatgattag ctgtgaggca ggggcctata
    19381 acaccattga tttggcatgg attgtgtcgc gtaaaaatcc gctggcctcg cgcccggttc
    19441 ggttgcgttt gccgttcaac aacgggcagg agacgaatga actggaactg atgaacgcaa
    19501 catttgatga gaaatcgcgt gaactggtga ccttagcgaa agggcgcgga ttaagcgatt
    19561 gtggcattca ggcgcgctgg cgctttgacg gtcagcgttt tcgcctggtg cgttacgccg
    19621 cagaacccac ctgcgataac tggcatgggc cagacgcctg gcccacgttg tggatcaccc
    19681 gttaaacaaa atggcgggga catgtaggcc ggataagacg ctgtaggccg gataagacgc
    19741 gtcagagtcg catccggcaa tcagcgtccg gcaatcagca tccggcgatc accatcaggc
    19801 acctttcact cccagcacct tatgcgcttt tgccacaata ttctcggcgg taaagccaaa
    19861 gaacgggaac agcttatccg ccggagcaga ttccccataa cctgtcatcc caacaatcgc
    19921 ccctttcaaa ccaacatact tgtaccagta atcggcaata cctgcttcca ccgccacgcg
    19981 agccgcaacg ttagaaggca acaccgactc ccgatattcc tcatcctggg cgtcgaaaat
    20041 atcagtcgag ggcagggaaa ctacgcgtac attgcgacct tctcccgcta atttctctgc
    20101 cgcttgcagg gggatttcca tctctgaacc ggtggcaatc aaaataatat ctggcttacc
    20161 gccgctgtct ttcagcacat aaccgccacg ggcaatctct tttacctgat ccggcgtacg
    20221 ttccacctgg gccagattct gccttgagag gatcagtgcc gtcggtccgt tgtggcgctc
    20281 aaccgccagc ttccagccca ccgccgcttc cacctgatcg cacggtcgcc aggtgctgaa
    20341 atttggcgtt aagcgcaggc tggccagttg ctcaacagcc tggtgcgtcg gaccatcttc
    20401 gcccaggcca attgagtcgt gggtataaac cataatctgc cgcgctttca tcagtgccgc
    20461 catccgcgcg gcgttacggg cgtattcaac aaacatcagg aacgtggcgg tatacggcac
    20521 aaagccgccg tggtgcgcga tgccgttggc tatagcggtc atgccaaatt cacgcacccc
    20581 gtagtgaatg tagttgcccg ccggatcttc cttcagcgaa acagaacctt tccagatggt
    20641 caggttgctg gagccagatc cgccgaaccg ccgagcaact caggcagcat cggc
//
LOCUS       DAJVTY010000061        20396 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVTY010000061 DAJVTY010000000
VERSION     DAJVTY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20396
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.061"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            117..932
                     /gene="mepH"
                     /locus_tag="OQQ66_002021"
     CDS             117..932
                     /gene="mepH"
                     /locus_tag="OQQ66_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1904961.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1060..1641
                     /gene="sodB"
                     /locus_tag="OQQ66_002022"
     CDS             1060..1641
                     /gene="sodB"
                     /locus_tag="OQQ66_002022"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1904962.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1787..2962)
                     /locus_tag="OQQ66_002023"
     CDS             complement(1787..2962)
                     /locus_tag="OQQ66_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1904963.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3129..3218)
                     /gene="ynhF"
                     /locus_tag="OQQ66_002024"
     CDS             complement(3129..3218)
                     /gene="ynhF"
                     /locus_tag="OQQ66_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1904964.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3517..4542
                     /gene="purR"
                     /locus_tag="OQQ66_002025"
     CDS             3517..4542
                     /gene="purR"
                     /locus_tag="OQQ66_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1904965.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4539..5471)
                     /locus_tag="OQQ66_002026"
     CDS             complement(4539..5471)
                     /locus_tag="OQQ66_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1904966.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5584..6795
                     /locus_tag="OQQ66_002027"
     CDS             5584..6795
                     /locus_tag="OQQ66_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1904967.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7086..8234
                     /gene="cfa"
                     /locus_tag="OQQ66_002028"
     CDS             7086..8234
                     /gene="cfa"
                     /locus_tag="OQQ66_002028"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1904968.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8274..8915)
                     /gene="ribE"
                     /locus_tag="OQQ66_002029"
     CDS             complement(8274..8915)
                     /gene="ribE"
                     /locus_tag="OQQ66_002029"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1904969.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9130..10503
                     /gene="mdtK"
                     /locus_tag="OQQ66_002030"
     CDS             9130..10503
                     /gene="mdtK"
                     /locus_tag="OQQ66_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1904970.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10544..11800)
                     /locus_tag="OQQ66_002031"
     CDS             complement(10544..11800)
                     /locus_tag="OQQ66_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1904971.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12108..12184
                     /locus_tag="OQQ66_002032"
     tRNA            12108..12184
                     /locus_tag="OQQ66_002032"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12142..12144,aa:Val,seq:gac)
     gene            12189..12265
                     /locus_tag="OQQ66_002033"
     tRNA            12189..12265
                     /locus_tag="OQQ66_002033"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12223..12225,aa:Val,seq:gac)
     gene            12373..12678
                     /locus_tag="OQQ66_002034"
     CDS             12373..12678
                     /locus_tag="OQQ66_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1904972.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12804..14408
                     /locus_tag="OQQ66_002035"
     CDS             12804..14408
                     /locus_tag="OQQ66_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1904973.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQCLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFVQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14420..15232)
                     /locus_tag="OQQ66_002036"
     CDS             complement(14420..15232)
                     /locus_tag="OQQ66_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904974.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFRRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIAIAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGSGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15236..16021)
                     /locus_tag="OQQ66_002037"
     CDS             complement(15236..16021)
                     /locus_tag="OQQ66_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1904975.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16018..16686)
                     /locus_tag="OQQ66_002038"
     CDS             complement(16018..16686)
                     /locus_tag="OQQ66_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1904976.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16750..17388)
                     /locus_tag="OQQ66_002039"
     CDS             complement(16750..17388)
                     /locus_tag="OQQ66_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1904977.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAELYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17401..19503)
                     /locus_tag="OQQ66_002040"
     CDS             complement(17401..19503)
                     /locus_tag="OQQ66_002040"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1904978.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKCVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGSPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(19473..20150)
                     /locus_tag="OQQ66_002041"
     CDS             complement(19473..20150)
                     /locus_tag="OQQ66_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1904979.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITVRILQRKKSWL
                     TVGQVIY"
BASE COUNT         5107 a         5308 c         4763 g         5218 t
ORIGIN      
        1 aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat tagaatcatc
       61 aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga cgctcagtgg
      121 cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc ctgcctttaa
      181 cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact catatcacca
      241 aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa acagcgaaaa
      301 aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa aaatcttcca
      361 ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag
      421 ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca
      481 aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa aaagctacga
      541 aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggtggcagct
      601 caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtga
      661 aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca gcgccaatcg
      721 aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggcacag
      781 ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgcacaggtc
      841 aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat gttggcgctc
      901 gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg
      961 gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc
     1021 tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca
     1081 ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac
     1141 tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg
     1201 tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac
     1261 gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc
     1321 gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc
     1381 aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg
     1441 aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccgctgacc
     1501 accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta catcgactat
     1561 cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta
     1621 gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgt
     1681 ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg
     1741 tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa
     1801 cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg
     1861 cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac
     1921 caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag
     1981 acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc ggtacgaccg
     2041 caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca
     2101 ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc aacgtgccgt
     2161 taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat ccgacaccaa
     2221 tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg
     2281 gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc gtcagcaatg
     2341 cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc
     2401 ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg
     2461 ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca
     2521 cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt
     2581 tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat gctccgtgat
     2641 tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg atggcggaaa
     2701 gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat
     2761 gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca taggcactga
     2821 ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc
     2881 ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc gccagcgcca
     2941 gcaacggata gttaattttc atgccttact ctccacctct tcgcgtcatt acgcgatatt
     3001 cattaaagtg gcgaaagcat gacagcaatc acaaaaaaat gaaaataaca aaaagagaaa
     3061 acacttttgc cattttgcta acaaacagga aggagatgcg agggagaacg cgctccctcg
     3121 agaggaaatc agtgcagcgc ggcagtcaaa cccacggcca cgatcaaacc gaggacgata
     3181 atcgttgtta ccagtgaaaa tttaaggtcg gtgctcatca agttttctcc ttttttatta
     3241 ccacacaaat agtgatatta cgcattttta cacactgtga tgaaaaaatc tcccgtcatt
     3301 tataatgata agtgttttta ccacttcccc ttttcgtcaa gatcggccaa aattccacgc
     3361 ttacactatt tgcgtactgg ccattgaccc cttcctgacg ctccgtgtcg tttttccggc
     3421 gtaccgcaac acttttgttg tgcgtaaggt gtgtaaaggc aaacgtttac cttgcgattt
     3481 tgcaggagct gaagttaggg tctggagtga aatggaatgg caacaataaa agatgtagcg
     3541 aaacgagcaa acgtttccac tacaactgtg tcacacgtaa tcaacaaaac acgtttcgtc
     3601 gctgaagaaa cgcgcaacgc cgtgtgggca gcgattaaag aattacacta ctcccctagc
     3661 gcggtggcgc gtagcctgaa ggttaaccac accaagtcta tcggtttgct ggcgaccagc
     3721 agcgaagcgg cctattttgc cgagatcatt gaagcggttg aaaaaaattg cttccagaaa
     3781 ggttacaccc tgattctggg taatgcgtgg aacaatcttg agaaacagcg ggcttatctg
     3841 tcgatgatgg cgcaaaaacg cgtcgatggt ctgctggtga tgtgttctga gtacccagag
     3901 ccgttgctgg cgatgctgga agagtatcgc catatcccaa tggtggtgat ggactggggt
     3961 gaagcaaaag ctgacttcac cgatgcggtc attgataacg cgttcgaagg cggctacatg
     4021 gccgggcgtt atctgattga acgcggtcac cgcgaaatcg gcgtcatccc cggcccgctg
     4081 gaacgtaaca ccggcgcagg ccgccttgcc ggttttatga aggcgatgga agaagcgatg
     4141 atcaaggtgc cggaaagctg gattgttcag ggtgactttg aacccgaatc tggttatcgc
     4201 gccatgcagc aaatactgtc gcagccgcat cgccctactg ccgtcttctg tggtggcgat
     4261 atcatggcaa tgggcgcact ttgtgctgct gatgaaatgg gtctgcgcgt cccgcaggat
     4321 gtttcgctga tcggttatga taacgtgcgc aacgcccgct attttacgcc ggcgctgacc
     4381 acgatccacc agccaaaaga ttcgctgggt gaaacagcgt tcaacatgct gttggatcgt
     4441 atcgtcaaca aacgtgaaga accgcagtcc attgaagtgc atccgcgctt gattgaacgc
     4501 cgctccgtgg ctgacggccc gttccgcgac tatcgtcgtt aatcacccgt tgcgggagtc
     4561 tcttccggct cccgcagcca ctccttattc agcgtctcac tatcgccgag atactcaagc
     4621 agccaggtta acgcaggcga catatcattt tgctgccatg tcagacaaca tgccgaatcc
     4681 gggaaggggt tttccaattc aagtgctacc cacttcccct cattaagcca cggtttggcg
     4741 aaatgtgttg gcaccatccc tatgcataat cctgccgaga tacaggttgc cgatgattcc
     4801 cagtcaggca cgacaactct tttttggtta tccagtaacc aggtaatacg tttaggtagc
     4861 gttcgcgagg tgtcttcgcg caccaacgac ggccagttgc gcaacgtatc atcgctgaat
     4921 gggccatcca tcaacgccag cgggtggtgg ctggcaacaa cgcaactcca gcttagcatc
     4981 cccatatccc ggaaggcata acgaccgcct accggaatcg cgcgtgttgc gccaatcgcc
     5041 agttccacgc gcccgtcgga aagcgcatcc cagacaccgt tgaacacttc ctgaaagaca
     5101 agaagttcga catcatcaaa atggcgataa aaatcaacga tcatctgccg tgtacgttct
     5161 ggcctgacaa tattatccac tgcgatagct aactgaccgc gccagccgtt cgctatctgc
     5221 tgacattgct ggcgggtgat ctgcattttt ttgacaacag agcgcccttc tttgagaaac
     5281 cacgctccag cagcggtcag ctccacatca cggtgccgtc gttcaaagag cggcaccgcc
     5341 agccactctt ccagctgacg cacggtatag ctgaccgcag aaggaacgcg atgcagctcc
     5401 tgtgccgcag cgctaaaact accattacgc gctaccgcat caacaacttc gagtgaatat
     5461 tctgaccaca tagtctgcct gcaaaatttt tgaaatcagt catcaaatat taccgtttca
     5521 caacactaat ttcactccct acactttgcg gcggtgttta attgagagat ttagagaata
     5581 tacatgcaac ctgggaaaag atttttagtc tggctggcgg gtttgagcgt actcggtttt
     5641 ctggcaaccg atatgtatct gcctgctttc gccgccatac aggccgacct gcaaacgcct
     5701 gcgtctgctg tcagtgccag ccttagtctg ttccttgccg gttttgccgc agcccagctt
     5761 ctgtgggggc cactctccga ccgttatggt cgtaaaccgg tgttattaat cggcctgaca
     5821 atttttgcgt taggtagtct ggggatgctg tgggtagaaa acgccgctac gctgctggta
     5881 ttgcgttttg tacaggctgt gggtgtctgc gccgcggcgg ttatctggca agcgttagtg
     5941 acggattatt atccttcaca gaaagttaac cgaatttttg cgaccatcat gccgctggtg
     6001 ggtctatctc cggccctggc tcctctgtta ggaagctggc tgctggtcca tttttcctgg
     6061 caggcgattt tcgccaccct gtttgccatt accgtggtgc tgattctgcc tattttctgg
     6121 ctcaaaccca cgacgaaggc cggtaacaat agtcaggatg gtctgacctt taccgacctg
     6181 ctacgttcta aaacctatcg cggcaacgtg ctgatatacg cggcctgttc agccagtttt
     6241 tttgcatggc tgaccggttc accgttcatc cttagtgaaa tgggctacag cccggcagtt
     6301 attggtttaa gttatgtccc gcaaactatc gcgtttctga ttggtggtta tggctgtcgc
     6361 gccgcgctgc agaaatggca aggcaagcag ttattaccgt ggttgctggt gctgtttgct
     6421 gtcagcgtca ttgcgacctg ggctgcgggc ttcattagcc atgtgtcgct ggtcgaaatc
     6481 ctgatcccat tctgtgtgat ggcgattgcc aatggcgcga tctaccctat tgttgtcgcc
     6541 caggcgctgc gtcccttccc acacgcaact ggtcgcgccg cagcgttgca gaacactcta
     6601 caactgggtc tgtgcttcct cgcaagtctg gtagtttcct ggcttattag tatcagcacg
     6661 ccattgctca ccaccaccag cgtgatgtta tcaacagtag tgctggtcgc gctgggttac
     6721 atgatgcaac gttgtgaaga agctggctgc cagaatcatg gcaatgccga agtcgctcat
     6781 agcgaatcac actgatctat atcgatatac ttatacttag gctgctaaca aaattttgtt
     6841 gtatgattga aattagcggc ctatactaat ttcgagttgt taaagctacg ataaatatta
     6901 tgtttttacg gggacaggat cgttcccgac tcactatgga tggtcatttc ggcaagggtt
     6961 cctcctttcc ctctgttcta cgtcggatta tagactcgcg gttttttctg cgagatttct
     7021 cacaaagccc aaaaagcgtc tacgctgttt taaggttctg atcaccgacc agtgatggag
     7081 aaactatgag ttcatcgtgt atagaagaag tcagcgtacc ggatgacaac tggtaccgta
     7141 tcgccaacga attacttagc cgtgccggta tagccattaa cggttctgcc ccggcggata
     7201 ttcgtgtgaa aaaccccgat ttttttaaac gcgttctgca agaaggctct ttggggttag
     7261 gcgaaagtta tatggatggc tggtgggaat gtgaccgact ggatatgttt tttagcaaag
     7321 tcttacgcgc aggtctcgag aaccaactcc cccatcattt caaagacacg ctgcgtattg
     7381 ccggcgctcg tctcttcaat ctgcagagta aaaaacgtgc ctggatagtc ggcaaagagc
     7441 attacgattt gggtaatgac ttgttcagcc gcatgcttga tcccttcatg caatattcct
     7501 gcgcttactg gaaagatgcc gataatctgg aatctgccca gcaggcgaag ctcaaaatga
     7561 tttgtgaaaa attgcagtta aaaccaggga tgcgcgtact ggatattggc tgcggctggg
     7621 gcggactggc acactacatg gcatctaatt atgacgtaag catggtgggc gtcaccattt
     7681 ctgccgaaca gcaaaaaatg gctcaggaac gctgtgaagg cctggatgtc accattttgc
     7741 tgcaagatta tcgtgacctg aacgaccagt ttgatcgtat tgtttctgtg gggatgttcg
     7801 agcacgtcgg accgaaaaat tacgatacct attttgcggt ggtggatcgt aatttgaaac
     7861 cggaaggcat attcctgctc catactatcg gttcgaaaaa aaccgatctg aatgttgatc
     7921 cctggattaa taaatatatt tttccgaacg gttgcctgcc ctctgtacgc cagattgctc
     7981 agtccagcga accccacttt gtgatggaag actggcataa cttcggtgct gattacgata
     8041 ctacgttgat ggcgtggtat gaacgattcc tcgccgcatg gccagaaatt gcggataact
     8101 acagtgaacg ctttaaacgg atgtttacct attatctgaa tgcctgcgca ggtgctttcc
     8161 gcgcccgtga tattcagctc tggcaggtcg tgttctcacg cggtgttgaa aacggccttc
     8221 gagtcgctcg ctaaaggcta ttctatcgcc ccctctccgg gggcgatttc agatcaggct
     8281 tctgtacctg gttgattcat ggcattttct cgtgccgcca gcacacgttc taccgtatct
     8341 accactgcct gagtttgtgg atcaatttca atgttgacgc gtgcgccgag ttttttcttc
     8401 ccaagagtcg tgcgttccag agtttccgga attaaatgga cgcaaaaacg cgttggcgtg
     8461 acttcgccga cggtcaggct aataccgtcg atgccaataa atcctttgta caggatatat
     8521 ttcatcaact gactatcctg gactttaaac cagatctggc gattattttc cgaggttaat
     8581 attttcgcca cttcagcggt ggtcataata tgacctgaca ttaagtgtcc gccaatttca
     8641 tcactgaatt tcgccgcacg ctcaacgttt acccaatccc ccacttttaa atcgccaaga
     8701 ttggtaatgc gtaacgtttc tttcatcagg tcaaaactga catggttgcc gttaatttcc
     8761 gtcacggtca ggcagcaacc gttatgcgcc acggaagcac cggtttccag gccgtccagc
     8821 atgtggtcgg gtaactccac cacatgcgta cgaaaatttg gtttctcgtc aatcgacacc
     8881 agttttgcgg tgccctgtac aatccccgta aacatactta caactcctga aatcagttaa
     8941 gacattctgt tcagcacaat agcaggtggc aaacgccctt accagtgaag gggtaagaat
     9001 gactattttt tcactggaga attaataaat cctcgctaca atagactgaa tttcccctgc
     9061 ttcttctttt tgctgcccat tcaggcggct ttttagtctc tcatataact acaaataaaa
     9121 ggtgttcacg tgcagaagta tatcagtgaa gcgcgtctgt tattagcatt agcaatcccg
     9181 gtgattctcg cgcaaatcgc ccaaactgcg atgggttttg tcgataccgt gatggcgggc
     9241 ggctatagtg ccaccgacat ggcggcggtc gctatcggta cttctatctg gcttccggcg
     9301 atcctctttg gtcacggact gctgctggca ttaacgccgg ttatcgcgca attaaatggt
     9361 tccggtcgac gtgagcgcat tgcgcatcag gtgcgacaag gtttctggct ggcaggtttt
     9421 gtttccgttc tcattatgct ggtgctgtgg aatgcaggtt acattatccg ctccatggaa
     9481 aacatcgatc cggctctggc ggacaaagcc gtgggttatc tgcgtgcgtt gttgtggggc
     9541 gcgccgggct atctgttctt ccaggttgcc cgtaaccaat gtgaaggtct ggcaaaaacc
     9601 aagccgggta tggtaatggg ctttatcggc ctgctggtga acattccggt gaactatatc
     9661 tttatttatg gtcatttcgg tatgcctgag ctcggtggcg ttggttgtgg cgtggctact
     9721 gcggcggtgt attgggtcat gttccttgcc atggtttctt acattaaacg cgcccgctcc
     9781 atgcgcgata ttcgtaacga aaaaggcacc gcgaaacccg atcctgcggt tatgaaacga
     9841 ctgattcaac tcggtttacc gattgcgctg gcactattct ttgaagtgac actgtttgcc
     9901 gtcgtggctc tgttagtgtc tccgctcggt attgttgatg tcgcaggaca ccagattgcc
     9961 ctgaacttta gttcactgat gtttgtgctt ccaatgtcgc tggcggcagc ggtaactatc
    10021 cgcgtaggtt atcgtctggg tcagggctca acgctggatg cgcaaaccgc tgcgcggacc
    10081 gggcttatgg tgggtgtctg tatggcaacc ctgacggcca ttttcacggt ttcactccgg
    10141 gagcaaatcg ccctgttgta caacgacaat cccgaggttg taacgctggc tgcgcatttg
    10201 atgttgctgg cggcggtata tcagatttct gactcaatcc aggtgattgg cagtgggatt
    10261 ttgcgtggtt ataaagatac gcgttccatt ttctatatca cctttacagc ttactgggtg
    10321 ctgggcttgc caagcggcta tattctggca ctgaccgatc tggtcgttga acctatgggg
    10381 ccagcaggct tctggatagg ctttattatc ggcctgacat cggcagccat tatgatgatg
    10441 ttgcgtatgc ggttcctgca acgtctgccg tcagttatca ttctgcaacg agcatcccgc
    10501 taataaagac aaggcgcaac cttcacgggt tgcgcctgtg tttttacgca ggctggagcg
    10561 ttgcgccaat cccgtcttcg tctggctgta atttcagagc gttgcacaga gtttgccgac
    10621 tggcatctgt tatctcaacg tgtatatcac cggtaacttc ccctttcaca ttgatgaagt
    10681 caggtgagat ttctttcgca ctggtttgca ggcaaatcgc tccctctccg cgcaggcgtt
    10741 tcactttcag ttgcacatgc gttgccgcac cagtgatcag ccacagtttt ccgccattca
    10801 gattgatgtt accaacaata aaagtgacac cgtcaccttt taccgtactg ccgtcgagaa
    10861 tcaacacacc gccctgctgt acagtaatcg ttgattgttg cagatctgcg ccatagccaa
    10921 taatcattga agcgccgtcc agtaatgcaa cactcccggt cagttcaacc ggtgcgcgat
    10981 cttcctctac gacaaaattt tcgtccgcgc tggtgggtga caacaggatc actgttccac
    11041 cttgtattac cgcattttcc agcgtaccgg catgcacttc agcccgacca cccacattca
    11101 cggacaggtt ttgcgtctta ccggaactgt agagctgaag gccatccgtc cccagacgat
    11161 aaatggatcc attctcaaca atggtgtcag agtcttcacc accgagatta ttcatggtgc
    11221 caccagcaag caacgtatca ctggctttcc cggccagcat aaaaacgccg ccctgattaa
    11281 tctcacaatt gctggcttcc ccacctggat acaccaacaa ttcaccacct tcatctacca
    11341 cgaccgctga ggttgtccca ttaaccaaca ggccgccctc ttgatcgaga atgatttttt
    11401 ccgcgcgatg tccttccagt acacgcaggt taccgccatt ttccagcaac aaaccgcagg
    11461 cataaccctg atcaacgcta aattcacctt cgggatggcg gccattaacc gtagcgagcg
    11521 tagagagact aattgcgcct ccctcggact gaacaatatc tgtggcaatg gcaccgtcgc
    11581 tgaccgatag cgtgccattg ttaccgtact gtgtgcattt ggcgatccca ccggttgcca
    11641 ctttcaacca gccagccccc gtgactacgg tattttcggc tcttccgcca gcttcaacaa
    11701 tcaactcgcc ttcagtaagt tgcgtcgcgc caatgacctc gccggtctta acaatttgca
    11761 cattcccgtc gctcatcaag tttttcgcat cagatcccat gatttattcc tttgctgcat
    11821 ctgtgtgcct ttattgctac ctaaatgtaa aggctacgga ggatttatcc acgacagatt
    11881 tgagatggtg gcaaacaact ctgtttaaac tctgatacac gaattattgg gttgtatcag
    11941 atgtaaatgc gatcctgaat aaaaatcgcc cttgcaaatc aacaaaatat agccaatttg
    12001 cttaaaagct ctgcaaacgg tcgaaatagc gcagaaaatt acgttttgcc tcttgccacc
    12061 ttcccactct gccgctaata ttcgtccccg ttgtcaccta caacgttgcg ttcatagctc
    12121 agttggttag agcaccacct tgacatggtg ggggtcgttg gttcgagtcc aattgaacgc
    12181 accatcctgc gtccgtagct cagttggtta gagcaccacc ttgacatggt gggggtcggt
    12241 ggttcgagtc cactcggacg caccagattt tcttaatctg gtcttctcct ttttccctct
    12301 gtttcttctc tgtatccaat acgttaaaag atttacactg tcttcgtatg cgttatcaga
    12361 aggagaatcg ctatggcaac tttgttacaa cttcattttg cttttaatgg cccatttggc
    12421 gacgcaatgg ctgagcagct taaaccgctt gctgagtcga ttaatcagga acctggtttt
    12481 ctgtggaagg tatggacaga aagtgaaaag aaccacgaag ccggtgggat ctatcttttt
    12541 actgatgaaa aaagcgccct tgcctatctg gaaaaacata ctgcccgact taaaaatctc
    12601 ggcgttgagg aagttgtcgc caaagttttc gatgtcaatg agccactgag tcaaatcaat
    12661 caggcaaaac tcgcctgaca gaatttaatc aagggcggtt agcgcccttt tcatccctgt
    12721 ctgaaatttc tcaaattcta aaaatctcaa ccaaacttat ctgataacac taaattcgaa
    12781 agaatgcgta caggtaagta acaatgaaaa aaattgctat tgtgggtgcc gggcctacgg
    12841 ggatctacac cttattctcg cttctacagc aacaaactcc actttctatt tctatcttcg
    12901 agcaggctga cgaggccggt gtcgggatgc catacagtga tgaggaaaac tcaaaaatga
    12961 tgctggcaaa tattgccagt attgaaatac cgccgattaa ttgtacgtat ctcgaatggc
    13021 tacaaaagca agaagccagc catctccagc gttatggcgt taaaaaagaa accttgcacg
    13081 atcgtcagtg tttaccgcga attctgctgg gcgaatattt ccgcgatcaa tttttacgat
    13141 tagtagacca ggcacgacag caaaaatttg cagtggctgt ttatgaatca tgccaggtta
    13201 ccgatctgca aattacaaat gctggcgtca tgctcgctac aaatcaggat ttacccagcg
    13261 agacgtttga tttagcggtg attgccacgg gtcacgtctg gcctgatgaa gaagaagcaa
    13321 ccagaacgta ttttcccagt ccgtggtcag gcctgatgga agcaaaggtc gatgcgtgta
    13381 acgtgggtat tatgggaaca tccttgagcg gactggatgc ggcaatggca gtggctattc
    13441 agcatggttc gttcattgaa gatgataaac aacacgtcgt ttttaaccgc gataacgcaa
    13501 gtgaaaagct aaatattacg ttgatgtcgc gcacgggtat tttacccgaa gccgatttct
    13561 attgccctat tccctacgag cccttacaca tcgtcaccga tcaggcatta aatgctgaga
    13621 ttcaaaaagg tgaagagggc cttttggatc gggtatttag attgatagta gaggaaatca
    13681 agtttgctga tccagactgg agccaacgca tagccttaga gagcctgaat gtcgattcct
    13741 ttgttcaagc ctggtttgcc gagcgcaaac aacgcgaccc atttgactgg gcagaaaaaa
    13801 atctccagga agtcgaacgc aataaacgag aaaaacatac tgttccctgg cgttatgtca
    13861 ttctgcgcct gcatgaagcc gtacaggaaa ttgttccaca tctgaatgaa cacgaccata
    13921 aacggttcag taaaggcctt gcccgggttt ttatcgataa ttatgcggca atcccttcag
    13981 agtctattcg tcggctgctg gccttacgtg aagcggggat cattcatatt ctcgccctcg
    14041 gtgaagacta cgaaatggaa attaatgagt cgcgcaccgt cctgaaaacg gaagacaaca
    14101 gctactcgtt tgacgttttt attgatgccc gcggacagcg tccgcttaaa gtgaaagata
    14161 ttcctttccc tgggctacgc gagcaattac agaaaacagg ggatgaaatc cctgatgttg
    14221 gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg ttcggcgcgt
    14281 tgccctggtt gatgcacgac cagcctttcg ttcagggact tacggcatgt gcagaaattg
    14341 gtgaggcgat ggctcgggcg gtcgtaaaac ctgcatcccg tgcacgtcgg cgtctttcgt
    14401 ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta agacgatagc
    14461 ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga actctttcgc
    14521 cgtcatgata tgccagtgcg gcgcttcccc ctgacccgag aataattgag tcgaccacgc
    14581 ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg tcgccgctgt
    14641 tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca ttactggcga
    14701 catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc gcaatccgac
    14761 tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc cgtgcgcgtt
    14821 caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg ctataaccca
    14881 tactggcaat ttgcgcggca tacccagcga ttgcgattcg ggcgttatcc tcacaacagc
    14941 cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc tctccggggt
    15001 ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca tccgccagcc
    15061 attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct tcctcaatca
    15121 gtgcatgatg aatatcggca taactaccgc cgcgtcggaa atggcgcagg aaccagcgat
    15181 acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc attcgttagt
    15241 gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc cccgtggtgc
    15301 aaagataaag atgtccgaag ataaaaaaga ggcttataaa tgccagagca aaatgcgcct
    15361 gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga tagagacaca
    15421 acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca taggcgacct
    15481 gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct tccccctgca
    15541 taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac ccctgacgac
    15601 gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag ccgagccagc
    15661 atgccagtaa caaaaatccg caaacttcat gcaccgcaac cagactttta accgcagttg
    15721 cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca aagagtaacg
    15781 cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc tcaccatgat
    15841 cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc agcaccaacc
    15901 acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat tcgggagtga
    15961 actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc gacgggttca
    16021 tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg gtttcccggc
    16081 accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa tttctgggct
    16141 atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa tgggcggaaa
    16201 gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt tagtgacagg
    16261 gttaaggtag cgaacctggt acggacaagc gccaatgcaa taactgcagc cgatgcactg
    16321 cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg tcgggcaaac
    16381 gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt gatactgcgt
    16441 ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc cctgagcggg
    16501 gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc accgtgactc
    16561 gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta acagagaaga
    16621 cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc gtcgagtgaa
    16681 ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc atgacatctc
    16741 cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata agccataagc
    16801 cttgccatag gtcataaatc gctcactaaa caagtacaac tccgccgtcg atgactccac
    16861 ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg cttcaatctg
    16921 cgcttcctgg aagtaatatg gcgcttgcat cagcatggcg actttgtaag gacgaggata
    16981 tgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct gctccacaac
    17041 ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat agtaatcatc
    17101 ctggcttccc gtcatacaag cgatatcagc gtagtctccg ttctgcttaa tgctttccag
    17161 caaaactgac agctcctctt ctgcaaccga atagggtggc gagagaaaca cggcgcgcgc
    17221 caccagctgc ccagccgcag aatgctggcg aatgaactct gccaggattt cagccggttc
    17281 tggttcggtt accgtatcaa cggggtgacg catcccctgt aaccattgcc gcttcgcctc
    17341 gtcaacttcg ctgactttcg ctaacggcaa ttcatcccga tggttcatct cacccattcc
    17401 ttacacaggc agtagattgt gtgctgccag atcggcggca atatcttcca gccccagacg
    17461 ctgcaatgtt tcgcgggtgg gactaccaag ctgtgggtcc cagcccattt ctttgtagaa
    17521 catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt cagtaaagac
    17581 cgggatctgc ggatccttgt cgaataccca ggaacagata agatcgtgtt cattacgcat
    17641 atctttggtt tgcataagtt ttaccgtgta ggcacgatgc aacgtaaaga tacgctctgc
    17701 agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga agaatttggc
    17761 ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg tcattggcca
    17821 gacccagtta cacagggtga cggcgttatg caaacagacc cgcaacagcg accacttagc
    17881 atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt aggcatcttc
    17941 agaaccaaaa agctctttcg ccacttcacg ttgcagtttc aatggcaagc cggaaccaat
    18001 aaagttgata tgggtatgtg tcatgcagtc acggttgaac atacagttaa caatggaacc
    18061 gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg accagagttt
    18121 atttttcgcg tagccccagt actcttcacc caaattccag cgttctgcga tggcatatga
    18181 accatcagcc aggtgactca gctcacccac acgatgcgcc agacggtagt aaaaatcttt
    18241 aatgaaatta acgtcacccg cttccagttg atcccagtga atttccgcat actcttcagc
    18301 aggcagaaca cacttgaaca cccctttgct gtaacaataa gtaaagtcgc gatgcaactg
    18361 cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca agttaccaat
    18421 cacacggcca tcgtctttat cttcaaaatc tttcggaccg ttcgggaaga tggtggtatg
    18481 gacaaagttg gctacacaag tgttaccacc tgtgctgggc acgccaaact ctttcacccg
    18541 aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccac tcatttttac
    18601 tgtgtatttc tctgccgccg gacctaagtc aaaaacggat ttataggtac gaaagccgac
    18661 cgtgttctga ttgcctggcg gaatttcccc cgtttcaatc ggaccacctt cagccgcgcc
    18721 ccaaaacagc cctttacgtg ctgtccaacg tgacttggga tctgaatact ctgcccacga
    18781 ttgcggcgta cttggcacga catggttgtt attcgcacca ataagttcag tcatcatgta
    18841 atcgttgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg tcccttcaac
    18901 cgcaatcgct ttcaggtttt tcgaacccat aattgcgcca gttcccgcac cgccgctgtg
    18961 gttacggcta ttcaacatgc cagagagagg aacaaggttt tccccagcct ggccaatagc
    19021 cgccacacag gtttccggac tggtcaatcg acaaatttct tccgtcgttg cgcgcgtccc
    19081 ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa tcttcagcca
    19141 taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga atttcatttg
    19201 cgctgcaaaa aagccaccca tatgggcatc gacaactaaa tttcctttgg taaaagtaga
    19261 aagtgaggtg atatttacgc gagaactaca gggggcacca gatccagtta atgggccggt
    19321 agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctggcggta cttcgtcata
    19381 cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac tggaatcttc
    19441 gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc aaccgttagc
    19501 catgattttt tcctttgcaa gattctcaca gtaatatctt tccactcgat aatttttaac
    19561 gcccctgttg ggcaggcgtt tgcacattca ccgcataaca cacatttcga ggatttttta
    19621 ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc gctacagcca
    19681 atacagcgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt aatcgcacca
    19741 atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag
    19801 ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa gaaataattg
    19861 cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa gttggtacaa
    19921 gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag cacccctttg
    19981 ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca acccaaaagt
    20041 gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat acgcaagaat
    20101 tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat ttatttcgca
    20161 tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat tcaattaata
    20221 cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg tatgagaaaa
    20281 gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt gattattctg
    20341 agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat aagtga
//
LOCUS       DAJVTY010000062        20295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVTY010000062 DAJVTY010000000
VERSION     DAJVTY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20295
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.062"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(46..471)
                     /gene="nudI"
                     /locus_tag="OQQ66_002042"
     CDS             complement(46..471)
                     /gene="nudI"
                     /locus_tag="OQQ66_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1904980.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            750..1292
                     /locus_tag="OQQ66_002043"
     CDS             750..1292
                     /locus_tag="OQQ66_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1904981.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1392..2594
                     /locus_tag="OQQ66_002044"
                     /pseudo
     CDS             1392..2594
                     /locus_tag="OQQ66_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2814..3596
                     /locus_tag="OQQ66_002045"
     CDS             2814..3596
                     /locus_tag="OQQ66_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS1904982.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3611..4816
                     /gene="rhmD"
                     /locus_tag="OQQ66_002046"
     CDS             3611..4816
                     /gene="rhmD"
                     /locus_tag="OQQ66_002046"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCS1904983.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4873..6162
                     /locus_tag="OQQ66_002047"
     CDS             4873..6162
                     /locus_tag="OQQ66_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1904984.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6180..6983
                     /gene="yfaU"
                     /locus_tag="OQQ66_002048"
     CDS             6180..6983
                     /gene="yfaU"
                     /locus_tag="OQQ66_002048"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS1904985.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVSSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7024..7926)
                     /locus_tag="OQQ66_002049"
     CDS             complement(7024..7926)
                     /locus_tag="OQQ66_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1904986.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8119..9309)
                     /gene="glpC"
                     /locus_tag="OQQ66_002050"
     CDS             complement(8119..9309)
                     /gene="glpC"
                     /locus_tag="OQQ66_002050"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1904987.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9306..10565)
                     /gene="glpB"
                     /locus_tag="OQQ66_002051"
     CDS             complement(9306..10565)
                     /gene="glpB"
                     /locus_tag="OQQ66_002051"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1904988.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10555..12183)
                     /gene="glpA"
                     /locus_tag="OQQ66_002052"
     CDS             complement(10555..12183)
                     /gene="glpA"
                     /locus_tag="OQQ66_002052"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1904989.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12456..13814
                     /gene="glpT"
                     /locus_tag="OQQ66_002053"
     CDS             12456..13814
                     /gene="glpT"
                     /locus_tag="OQQ66_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1904990.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13819..14895
                     /gene="glpQ"
                     /locus_tag="OQQ66_002054"
     CDS             13819..14895
                     /gene="glpQ"
                     /locus_tag="OQQ66_002054"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1904991.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14937..15179)
                     /locus_tag="OQQ66_002055"
                     /pseudo
     CDS             complement(14937..15179)
                     /locus_tag="OQQ66_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15358..16008
                     /gene="inaA"
                     /locus_tag="OQQ66_002056"
                     /pseudo
     CDS             15358..16008
                     /gene="inaA"
                     /locus_tag="OQQ66_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16062..16316)
                     /gene="yfaE"
                     /locus_tag="OQQ66_002057"
     CDS             complement(16062..16316)
                     /gene="yfaE"
                     /locus_tag="OQQ66_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1904992.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16316..17446)
                     /gene="nrdB"
                     /locus_tag="OQQ66_002058"
     CDS             complement(16316..17446)
                     /gene="nrdB"
                     /locus_tag="OQQ66_002058"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1904993.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17593..19878)
                     /gene="nrdA"
                     /locus_tag="OQQ66_002059"
     CDS             complement(17593..19878)
                     /gene="nrdA"
                     /locus_tag="OQQ66_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1904994.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4738 a         4952 c         5482 g         5123 t
ORIGIN      
        1 ccactaaatt ttcaatctga cagccagtga tggctgtcat cattgttaaa gaagaccttt
       61 caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat cttcaggttt
      121 tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg cagaaacgca
      181 gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat acgtcttggt
      241 gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct gttctcccag
      301 ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact ccacgccgcc
      361 acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt tgcacagcag
      421 ataagcacca tcattttgaa tcaaagggca tacaatagtc cgttgtcgca caggggtatt
      481 ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg aaagtgacag
      541 taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc ggtaaagtgt
      601 gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag tgactagatc
      661 ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc tttgggaatt
      721 ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca ggtctggccg
      781 gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag gcgggtaaag
      841 aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg ttaagcggta
      901 actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg ttgaatctgc
      961 ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac ccgaattacg
     1021 gcgatgaagg ttatgccgca gcggtaggag gtggtttgca gtggaaaatt ggcaacagct
     1081 tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt attcaaagtt
     1141 atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt attgaggcgg
     1201 gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc gtggctgacg
     1261 gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat tctgtcgcgc
     1321 cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga taacaaaagc
     1381 aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg ttacacgggc
     1441 aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag gggctgccat
     1501 tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt cttcgcgaac
     1561 gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc agcgatgatt
     1621 taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat gaagcctggc
     1681 tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg agcaaccgta
     1741 aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc accgcctgtg
     1801 gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta ccgtcagaat
     1861 ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt tctttaccac
     1921 agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat ctggcacaaa
     1981 gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctct tcaatgccga
     2041 ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa aaactgtggc
     2101 tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa ggactgcccg
     2161 tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc gagtaattca
     2221 ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg gcgtgtgaag
     2281 tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca gaacggcggg
     2341 ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa catctcaact
     2401 ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc actacgcgct
     2461 acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg ctgcgccgtt
     2521 ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt gagtccatga
     2581 ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca ttccccctcc
     2641 ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact gattcacaaa
     2701 tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg cttcacaatt
     2761 ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc agtatgttgg
     2821 aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctaaatttg attgcccgca
     2881 ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa agcacggcct
     2941 atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac caggaaaatt
     3001 tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag atggatctcc
     3061 gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg ttatgtcatc
     3121 tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca tcagccacta
     3181 tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga ataggtaaat
     3241 gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg ctggtctggg
     3301 agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa ctggcgcgta
     3361 ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc cgttgtgtcg
     3421 ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg gtcggtaccc
     3481 gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt gcctgcgcca
     3541 gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc tcataacaat
     3601 ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta ctggcggtgc
     3661 gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga atcactggat
     3721 agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt cacgccagtc
     3781 atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa acggccagac
     3841 cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa aacaccttaa
     3901 ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc aaatgctcaa
     3961 tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt cctgtgtcga
     4021 tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata aacttttagg
     4081 cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg atctggcaaa
     4141 agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg atggcgatgc
     4201 ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg gtgaagattt
     4261 ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca aactagccca
     4321 cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac agcagtatga
     4381 aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca gcggtgagca
     4441 ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca ttatgcagcc
     4501 ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa tcgccaaatc
     4561 cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg cggtgatcac
     4621 cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt caacgatgcg
     4681 tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc gtattcataa
     4741 atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca atttgaaacg
     4801 cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc cacatattct
     4861 aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa ccgtgtgcgt
     4921 ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc gaatatcggt
     4981 tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta tgcgctggga
     5041 gcaggcattt tctttgtggt atatgcgttt ctgggggttc cggcgaatct tttgatgcgc
     5101 aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt tctttcggca
     5161 gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct gcttggtgct
     5221 gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt tccgcagcgt
     5281 aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt aacactggga
     5341 tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc cggctggttc
     5401 tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac attcttttgg
     5461 cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac gttgcttatc
     5521 aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga tgcgctgcgt
     5581 aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc ggtttacgga
     5641 ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt gggctttaca
     5701 gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg gcttattccg
     5761 cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt actggcggcg
     5821 ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt agcgctgtgt
     5881 gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc gacacaactg
     5941 ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg tgcagtgggc
     6001 gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag cgatgcggca
     6061 ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt cactctgcgt
     6121 gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta aggaacacga
     6181 tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa gtgcaaattg
     6241 gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact tccggttatg
     6301 actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt tatcaccagc
     6361 tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa ggcagtaaat
     6421 cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg atggtcgata
     6481 ctgccgatca ggctcgtcag gtgtcgtctg ccacgcgcta tcctccctac ggtgagcgtg
     6541 gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat tacatggcgc
     6601 aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca ctggataacc
     6661 tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca gcggatcttt
     6721 ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga attattgaaa
     6781 ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct gtggctcctg
     6841 atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc gttgacacga
     6901 tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc aaaaatgggc
     6961 cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt gggcctgtgt
     7021 taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg cgagcacctg
     7081 gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt gttcatgcat
     7141 accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa tagtcattaa
     7201 tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg tgaaacggga
     7261 ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt cattagtgaa
     7321 gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc ggtcgcgaat
     7381 atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct catcgtcagg
     7441 tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg caagttgcgg
     7501 atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt cgccatgata
     7561 aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt gacgctgcat
     7621 ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag agctttgatg
     7681 ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc acaaaacatc
     7741 cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc gtaaggtttg
     7801 caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat cccgtgcggt
     7861 ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg ttgttgattc
     7921 ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt atcctaggcc
     7981 tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt cactgcgttg
     8041 caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc acatgcttac
     8101 cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg gatgttcgca
     8161 gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg tgaccaccag
     8221 atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt gtgaggtggg
     8281 gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg aatccagcac
     8341 cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg tccagcccat
     8401 tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt tcagcggtaa
     8461 cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga tatgatcgcg
     8521 caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg caaatgtaca
     8581 ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc ggatcgactc
     8641 tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga ttagcggtac
     8701 gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt tgagcacttt
     8761 aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt gaaagaaagc
     8821 gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc gacggaacgt
     8881 gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg catcaagcag
     8941 ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg gcgtggaaac
     9001 gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc gcgtggtgtc
     9061 atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg ggcaggcgac
     9121 ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg cgccatcttt
     9181 caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac cgggattcac
     9241 ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt cgaagctggt
     9301 gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg taaagcactg
     9361 acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc cagcaccgaa
     9421 ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag cgtctcatca
     9481 gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt ataccattca
     9541 ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat gccgttacgt
     9601 tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa atgtggacgt
     9661 agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt tttacaggtc
     9721 acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa ctggcgctgt
     9781 aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa aagcatcagt
     9841 gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag gccgaagcag
     9901 gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc aagtaacagc
     9961 ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa ttcggtggcg
    10021 ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac cgaaagatcc
    10081 agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc gcttatcccg
    10141 accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga acttagccag
    10201 gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac gctgccctgc
    10261 aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc gagcacgcgt
    10321 tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga ttccagtcca
    10381 ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc cagcgaaccg
    10441 gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag gccgtgtttt
    10501 tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac agtatcaaag
    10561 cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc gggtaaattc
    10621 gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc gttcgttaag
    10681 gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc gttgcagcag
    10741 tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca cacgggtacg
    10801 acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact gcacttcacc
    10861 cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt cgctcagcca
    10921 ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg caggcaggga
    10981 gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg ccagatcggc
    11041 agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag cccattcagc
    11101 catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt ccagaccatc
    11161 gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc tcggatcgtc
    11221 atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg ttttcgccat
    11281 cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg ccgtcactcg
    11341 attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca gcgaaatggt
    11401 atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga tcacatgctg
    11461 gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc gaatgcgcag
    11521 atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca cgacaggtgc
    11581 atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc acaccgtcgc
    11641 gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac cgtgttcttt
    11701 ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat ccggaacttt
    11761 caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt gctgcgggtc
    11821 tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg cctggaatga
    11881 gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc agtgacgtgc
    11941 aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg catcggttac
    12001 cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac cggttgcgat
    12061 gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc gggcaatccc
    12121 ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt cgcgagtttt
    12181 cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa catgaattgt
    12241 ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat gcgtcatttt
    12301 gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa tttatgaatg
    12361 ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat caggctgtta
    12421 atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt aaaccagcgc
    12481 cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga ttgcgctggc
    12541 aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt aagaactttg
    12601 cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta ggtttcgcgc
    12661 tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg gtatcagatc
    12721 gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca gtgatgttgt
    12781 ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta cttttgttcc
    12841 tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg gtgcactggt
    12901 ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac aacgttggtg
    12961 gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac tggcatgcgg
    13021 cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt gcgatgatgc
    13081 gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat gattatccgg
    13141 acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc atgcagtacg
    13201 tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt tatctgctgc
    13261 gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat ttcgcgctgg
    13321 ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc actctgctgt
    13381 gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc gttttcttta
    13441 tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt aacccaaccg
    13501 tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg atgctgatcg
    13561 gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg ggctttaccg
    13621 ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc tacaccgtgg
    13681 acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt ctggcggtta
    13741 tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta ctacaaaaac
    13801 gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc gatcatgatg
    13861 agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa aatagtcatc
    13921 gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa agcgatggcg
    13981 tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga cgaccatctg
    14041 gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg tttcccggat
    14101 cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga aattaagtcg
    14161 ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac ttatccgggg
    14221 cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga gattgaattt
    14281 gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga aatcaaagcg
    14341 ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga agtgctgaag
    14401 aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga tgctgatgag
    14461 ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa tctggtacag
    14521 ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg aagctgggtt
    14581 aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc ggaatatgca
    14641 gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc gggtaatatc
    14701 aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca tccttatacc
    14761 gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata tgatgctctg
    14821 tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc agtaaaattt
    14881 cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt ctcatatcaa
    14941 atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag tggactgcct
    15001 tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg ttcgagagga
    15061 acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa ggcgatgcct
    15121 aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca ttttatctgc
    15181 tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg aagggtaaaa
    15241 gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt cattaagatt
    15301 ttcctcaggt agccacctat agtcattgtc gttaataaaa acgacaggcg atagggtatg
    15361 gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg cgactgggtt
    15421 gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga gcgcaacggc
    15481 aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg ttatccgttc
    15541 ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg ggccggtgtc
    15601 atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg gcgagcgttg
    15661 ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc ccagcatgca
    15721 gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc gctggcgttt
    15781 aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca tatctatgtg
    15841 aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg tcgccgcttg
    15901 cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct ggagccaatc
    15961 cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc gttgtaggcc
    16021 agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc gatttcaata
    16081 tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa ggctaacggt
    16141 tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc gcagtaacct
    16201 tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag aagggaaggg
    16261 tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg ggccatcaga
    16321 gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg accagataag
    16381 aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc caagtgttga
    16441 tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact gcctgcatac
    16501 ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga ccaatcatcg
    16561 aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc tgaacaaaca
    16621 ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga tcgtccgcgc
    16681 cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct tcgtcgcggg
    16741 caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca aaggcaaagg
    16801 aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc atcaggcaaa
    16861 gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg ttaacggtgt
    16921 gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca tcgtaatagc
    16981 tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg tcaaacacaa
    17041 cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa tgaatcgttt
    17101 ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata agcggcaata
    17161 gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat ttcaggttgc
    17221 tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg tcgcgggaga
    17281 cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc ttttcgaaga
    17341 tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag aacatcggtt
    17401 ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt gtgtcctgtt
    17461 gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg cgacgctggc
    17521 gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca cgctgcatcc
    17581 ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct ggattgacgg
    17641 taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat acagcgtttt
    17701 gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg gcacttttcc
    17761 tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat cgataaattt
    17821 ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc acagcagctc
    17881 ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg cgcaaaatac cgtctttcga
    17941 cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt tagagatctg
    18001 cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc cgtgcgtttt
    18061 gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga tggcatccag
    18121 atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt cgttaaacca
    18181 cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt aatactgaat
    18241 cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc gtttaccgtg
    18301 cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac gacccatcgc
    18361 tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt caagtgcacg
    18421 aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc ctaggttgaa
    18481 agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt tcagcggttt
    18541 ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga tggccggatc
    18601 aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac cggtagacgc
    18661 acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct tgcggatgct
    18721 gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg cgaagaacgc
    18781 gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac ctttcagcag
    18841 acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca cgcggttgcc
    18901 ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat gccacatcgg
    18961 gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt tcaccgctgt
    19021 ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac cgcgaatcgg
    19081 gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct gggaaacgta
    19141 tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc actcgatcag
    19201 tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg gcgtcggcag
    19261 cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca ggcgcgtttc
    19321 acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact gggcgctctc
    19381 atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct gcttaacggc
    19441 agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct gcttgaactc
    19501 ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga ccattttcac
    19561 cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac gcaggtggaa
    19621 gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga tcaggtctgc
    19681 ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat aaaactgaat
    19741 gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg ccgcccaatc
    19801 cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc gctttgtcac
    19861 cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg caatagtttc
    19921 atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg ggtatgtttg
    19981 ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact ttgtggataa
    20041 ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc cgggaaagtc
    20101 aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa cggcagaata
    20161 ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg caaaaattga
    20221 tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata taagcgaatg
    20281 taagcgattg ttgat
//
LOCUS       DAJVTY010000063        20266 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVTY010000063 DAJVTY010000000
VERSION     DAJVTY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20266
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.063"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            133..336
                     /locus_tag="OQQ66_002060"
     CDS             133..336
                     /locus_tag="OQQ66_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1904995.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            344..1276
                     /gene="aaeA"
                     /locus_tag="OQQ66_002061"
                     /pseudo
     CDS             344..1276
                     /gene="aaeA"
                     /locus_tag="OQQ66_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            1282..3249
                     /gene="aaeB"
                     /locus_tag="OQQ66_002062"
     CDS             1282..3249
                     /gene="aaeB"
                     /locus_tag="OQQ66_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1904996.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTGLQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRLLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3341..3613
                     /locus_tag="OQQ66_002063"
     CDS             3341..3613
                     /locus_tag="OQQ66_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1904997.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3669..3932)
                     /gene="yhcN"
                     /locus_tag="OQQ66_002064"
     CDS             complement(3669..3932)
                     /gene="yhcN"
                     /locus_tag="OQQ66_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1904998.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4298..4768)
                     /gene="argR"
                     /locus_tag="OQQ66_002065"
     CDS             complement(4298..4768)
                     /gene="argR"
                     /locus_tag="OQQ66_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1904999.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5203..6141
                     /gene="mdh"
                     /locus_tag="OQQ66_002066"
     CDS             5203..6141
                     /gene="mdh"
                     /locus_tag="OQQ66_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1905000.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(6204..7271)
                     /gene="degS"
                     /locus_tag="OQQ66_002067"
     CDS             complement(6204..7271)
                     /gene="degS"
                     /locus_tag="OQQ66_002067"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1905001.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(7361..8728)
                     /gene="degQ"
                     /locus_tag="OQQ66_002068"
     CDS             complement(7361..8728)
                     /gene="degQ"
                     /locus_tag="OQQ66_002068"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS1905002.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(8882..9280)
                     /locus_tag="OQQ66_002069"
     CDS             complement(8882..9280)
                     /locus_tag="OQQ66_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1905003.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            9474..10601
                     /gene="zapE"
                     /locus_tag="OQQ66_002070"
     CDS             9474..10601
                     /gene="zapE"
                     /locus_tag="OQQ66_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1905004.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            10819..11247
                     /gene="rplM"
                     /locus_tag="OQQ66_002071"
     CDS             10819..11247
                     /gene="rplM"
                     /locus_tag="OQQ66_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1905005.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            11263..11655
                     /gene="rpsI"
                     /locus_tag="OQQ66_002072"
     CDS             11263..11655
                     /gene="rpsI"
                     /locus_tag="OQQ66_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1905006.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            12050..12688
                     /gene="sspA"
                     /locus_tag="OQQ66_002073"
     CDS             12050..12688
                     /gene="sspA"
                     /locus_tag="OQQ66_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1905007.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            12694..13191
                     /gene="sspB"
                     /locus_tag="OQQ66_002074"
     CDS             12694..13191
                     /gene="sspB"
                     /locus_tag="OQQ66_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1905008.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(13234..14600)
                     /gene="dcuC"
                     /locus_tag="OQQ66_002075"
                     /pseudo
     CDS             complement(13234..14600)
                     /gene="dcuC"
                     /locus_tag="OQQ66_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            14980..15771
                     /gene="nanR"
                     /locus_tag="OQQ66_002076"
     CDS             14980..15771
                     /gene="nanR"
                     /locus_tag="OQQ66_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1905009.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            15893..16786
                     /gene="nanA"
                     /locus_tag="OQQ66_002077"
     CDS             15893..16786
                     /gene="nanA"
                     /locus_tag="OQQ66_002077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1905010.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            16895..18385
                     /gene="nanT"
                     /locus_tag="OQQ66_002078"
     CDS             16895..18385
                     /gene="nanT"
                     /locus_tag="OQQ66_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1905011.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     SQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            18433..19122
                     /locus_tag="OQQ66_002079"
     CDS             18433..19122
                     /locus_tag="OQQ66_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1905012.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            19119..19994
                     /gene="nanK"
                     /locus_tag="OQQ66_002080"
     CDS             19119..19994
                     /gene="nanK"
                     /locus_tag="OQQ66_002080"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1905013.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            19991..>20266
                     /locus_tag="OQQ66_002081"
     CDS             19991..>20266
                     /locus_tag="OQQ66_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1905014.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQC"
BASE COUNT         4862 a         5051 c         5287 g         5066 t
ORIGIN      
        1 ataacagtta cgctaatata ttaaatatca atctacagca atgttgctct cgcccggctt
       61 tccatgccct tctctgcggc gacagatgct gaaaataata acgcctgctc tctctataca
      121 accaaggtca acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg
      181 atattttttg aattgctttt atcactggcg attttctggc tggtgcgccg ggtacttgtg
      241 ccaacaggta tctacgactt tgtctggcat ccggcgttgt tcaacaccgc gctctattgc
      301 tgcttgtttt atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa cactaataag
      361 aaaattctcc cgtacggcca tcacggtcgt attagtcatt ctggccttca tcgcaatttt
      421 taatgcctgg gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga
      481 cgtcattgcg atcgcgccgg acgtttctgg actcattacc caggtgaatg ttcatgataa
      541 ccagctggtg aaaaaaggac agatactgtt caccatcgac cagccgcgct atcaaaaggc
      601 gcttgaggaa gcgcaagccg atgttgctta ttatcaggta ctggcacagg agaaacgcca
      661 ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag agatcgacta
      721 ggctaacaac gtactacaaa cggttctgca tcagttagcg aaagcgcagg cgacccgcga
      781 tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg ccagcagatg gctgggtgac
      841 caacctcaac gtctataccg gtgagtttat tactcgagga tcaacggcgg ttgcgctggt
      901 gaaacagaac tccttctatg tactggccta tatggaagaa actaagctgg aaggggtgcg
      961 tccggggtat cgtgcagaga tcacgccgct tggcagtaac aaagtgctga aagggactgt
     1021 tgatagtgtt gccgcagggg tcaccaacgc cagcagcacg cgtgacgaca aagggatggc
     1081 gactatagac tctaaccttg aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg
     1141 tctcgacaac cagcaagaga acatctggcc tgcgggcacc actgctacag tggtggtcac
     1201 tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct
     1261 gcgtgagttt ggttaatcac gatgggtatt ttctccattg ctaaccaaca tattcgcttt
     1321 gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg ttggctttca cttccagctg
     1381 gaaacgccac gctgggcggt actgacagcg gcgattgttg ccgccggtcc ggcctttgct
     1441 gcgggaggtg aaccgtattc tggcgctatt cgttatcgtg gctttttgcg catcatcggc
     1501 acatttattg gctgtattgc cggactggtg atcatcattg cgatgatccg cgcaccatta
     1561 ttgatgattc tggtgtgctg tatctgggcc ggtttttgta cctggatatc ctcgctggta
     1621 cgaatagaaa actcgtacgc gtgggggctg gccggttata ccgcgctgat cattgtgatc
     1681 accattcagc cggaaccatt gcttacgccg cagtttgccg tcgaacgttg tagcgagatc
     1741 gttatcggta ttgtgtgtgc gattatagcg gatttgctct tttctccgcg atcgatcaaa
     1801 caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc
     1861 tgtatcaagc atggcgatgg tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc
     1921 accacggggc tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg
     1981 gccaatcgac gtttaaaagc gatcaatacg ctatcgctga cgctgattac ccaatcctgc
     2041 gaaacttatc ttattcagaa tacgcgcccg gaattgatca ctgatacttt ccgcgaattt
     2101 tttgacacgc cggtagaaac cgcgcaggac gtccacaagc agctcaaacg cctgcggaga
     2161 gttatcgcct ggaccgggga acgggaaacg cctgtcacca tttatagctg ggtcgcggcg
     2221 gcaacgcgtt atcagcttct caagcgcggc gttatcagta acacaaaaat caacgccacc
     2281 gaagaagaga tcctgcaagg cgaaccggaa gtcaaagtag agtcagccga acgtcatcat
     2341 gcgatggtta acttctggcg aaccacactt tcctgcattc tgggcacgct tttctggctg
     2401 tggacgggct ggacttccgg cagtggtgca atggtgatga ttgcggtagt gacgtcactg
     2461 gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact ttatctacgg gacgctggcc
     2521 gcgctgccgt tagggctgct ctactttttg gtgattatcc ctaataccca acagagcatg
     2581 ttgctgctgt gtattagcct ggcagtgctg ggatttttcc tcggtataga agtacagcaa
     2641 cggctactgg gctcgatggg ggcgctggcc agcaccataa atattatcgt gctggataac
     2701 ccgatgactt tccatttcag tcagtttctc gacagcgcat tagggcaaat cgtcggctgt
     2761 gtgctcgcgt tcaccgttat tttgcttgtg cgggataaat cgcgcgacag gaccggacgt
     2821 gtactgctta atcagtttgt ttctgccgct gtttccgcga tgactaccaa tgtggcacgt
     2881 cgtaaagaga accacctccc ggcactttat cagcagctgt ttttgctgat gaataagttc
     2941 ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg
     3001 cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc
     3061 acagcagacc atgtgatatc tgcccgtagc gatgataaac gtcgtcggta ctttggccag
     3121 ttgctggaag aacttgaaat ctaccaggaa aagctacgca tctggcaagc gccaccgcag
     3181 gtgacggaac cggttcatcg gctggcgggt atgctccata agtatcaaca tgcgttgacc
     3241 gatagttagg tcaaaaccaa cgccaaaagt gtcggttttt tcatggctat acttagcgat
     3301 gcacggcaga actcgccgcg aaacgtgacg gtggcaatag atgaatattt atacctttga
     3361 ttttgatgag attgagagtc aggaggattt ttatcgtgac tttagccaaa cctttggtct
     3421 ggcgaaagat aaggtacgcg atctcgactc actatgggat gtgttaatga acgatgtcct
     3481 gccgctacca cttgagattg aatttgttca tctgggagag aaaacgcgtc gccgttttgg
     3541 cgcgttaata ttgctgtttg atgaggcaga ggaagagctg gaagggcatt tgcgttttaa
     3601 tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc ggacaagacg
     3661 atgagggttt atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca
     3721 gtaatctggt aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgcata
     3781 tccattggcg aagacgccac accacttacg gatacggtcc cgattgcttc acgattttgt
     3841 gcttgtgcag catcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg
     3901 cttaatgcag ctacagtggt tttgattttc atgattttta cctcgacata atcttttagc
     3961 tgggcctttg ttttgtgacc catatcacaa aatcaagcac acactaatta ttgaactaat
     4021 taataccacg ctaactgttt cagttgatac taagtgttaa aaatgtgaat tttaataacg
     4081 aaaaagaatt aaaaacacgg tcatttattt ttaattctag tattttcaat tagataatga
     4141 attttgcata atgtgctttt tttaccgtat gtattcatta tgtgaatgac atgtcgcagt
     4201 aaaactgcac tattcgttaa attaatcgca ggggaaagca gggggttgag agggataagc
     4261 aacattttcc ccgccgccag aagcgacggg gcagagatta aagctcctgg tcgaacagct
     4321 ctaaaatcgc ttcgtacagg tctttgacgg tgaaaccgtt agcaggggtg gtaaagatgg
     4381 tgtcatcgcc agcgatggtg cccagaatac cttctgcttt gcccagtgag tccagcaggc
     4441 gagcaattaa ctgcgccgcg ccagggctgg tatgaatcac gacaactgca tcgttgtagt
     4501 cgatatccag caccagattc ttcaatggac tggaggtggt tggtacaccc agttcagctg
     4561 gcaggcagta aaccatttcc attttggcat tgcgtgtacg tacagcacca aacttggtca
     4621 acatccgcga gactttagac tgattaatat tgtcaaagcc ttgctcctgc aacgcggcga
     4681 cgatttcgcc ctgggagcta aatttctctt ctttaagtaa tgctttaaat gctttaacta
     4741 gttcttcttg cttagccgag cttcgcataa gtcacccgat atggtggttg atacaacatt
     4801 attgtgcata cagatgaatt tttatgcaaa cagtcaaccc tgaagaaggc tgaaataatg
     4861 ttatgaaaga gcgggatttt atcaaatttc gttattgaga aacatgcctg cgtcacggca
     4921 tgcaaattct gcttaaaagt aaattaattg ttatcaaatt gatgttgttt tggctgaacg
     4981 gtagggtata ttgtcaccac ctgttggaat gttgcgctaa tgcataagcg actgttaatt
     5041 acgtaagtta ggttcctgat tacggcaatt aaatgcataa acgctaaact tgcgtgacta
     5101 cacattcttg atatgtggtc attgtaaacg gcaattttgt ggattaaggt cgcggcagcg
     5161 gagcaacata tcttagttta tcaatataat aaggagttta ggatgaaagt cgcagtcctc
     5221 ggcgctgctg gcggtattgg ccaggcgctt gcactactgt taaaaaccca actgccttca
     5281 ggttcagaac tctctctgta tgatatcgct ccagtgactc ccggtgtggc tgtcgatctg
     5341 agccatatcc ctactgctgt gaaaatcaaa ggtttttctg gtgaagatgc gactccggcg
     5401 ctggaaggcg cagatgtcgt tcttatctct gcaggtgtag cgcgtaaacc gggtatggat
     5461 cgttccgacc tgtttaacgt taacgccggc atcgtgaaaa acctggtaca gcaagttgcg
     5521 aaaacctgcc cgaaagcgtg cattggtatt atcactaacc cggttaacac cacagttgca
     5581 attgctgctg aagtgctgaa aaaagccggt gtttatgaca aaaacaaact gttcggcgtt
     5641 accacgctgg atatcattcg ttccaacacc tttgttgcgg aactgaaagg caaacagcca
     5701 ggcgaagttg aagtgccggt tattggcggt cactctggtg ttaccattct gccgctgctg
     5761 tcacaggttc ctggcgttag ttttaccgag caggaagtgg ctgatctgac caaacgtatc
     5821 cagaacgcgg gtactgaggt ggttgaagcg aaagccggtg gcgggtctgc aaccctgtct
     5881 atgggccagg cagctgcacg ttttggtctg tctctggttc gtgcactgca gggcgaacaa
     5941 ggcgttgtcg aatgtgccta cgttgaaggc gacggtcagt acgcccgttt cttctctcaa
     6001 ccgctgctgc tgggtaaaaa cggcgtggaa gagcgtaaat ctatcggtac cctgagcgca
     6061 tttgaacaga acgcgctgga aggtatgctg gatacgctca agaaagatat cgccctgggc
     6121 gaagagttcg ttaataagta attgattagc gaataataaa aaaccggagc acagactccg
     6181 gttttttgtt ttgagcactc gacttaattg gttgccggat attcctgaat ggtgacctgc
     6241 agcgttaact gcttatcatc acgcatcact acaaccggga tcaccgaacc agggcgaatt
     6301 tctgccacct gatccatcgt ctccagagca gagatggccg gtttgttatc caccgaaata
     6361 atcagatcgt tgacctgaat acccgcattc gccgccgggc cgtcaggtga cacttcatta
     6421 accacgatcc cttgcagttg atctatacca ccgctctgcg cgtgcagtgg tgcgatctca
     6481 cgtccaccaa tgccaatgta gccgcggatc acgcgcccat cgcggatcag cttatccata
     6541 attttggttg ctaactggaa aggaatcgca aagccgatac cttccggcgt ttcgccatcg
     6601 ttactcttat caaacgacag cgtgttaatg cccatcagtt ctcccagcga gttcaccagc
     6661 gcgccgccag agttaccgtg gttaatggaa gcatcggttt gtaggaagtt ttgccgcccg
     6721 gtagggttca gaccgattcg ccccgtggca ctaataatcc cttgggtaat ggtctgcccg
     6781 aggttgtacg ggttaccgat cgccagtact acgtcgccaa tgtgcggtac gcgacgtgca
     6841 ttaattggaa tggtaggtaa accgccagtg gcattaattt taagtaccgc cagatcggtt
     6901 agagagtcag atcccaccag caatgcttca aatacacgtc catcctgtaa ggcgacgatg
     6961 atctgatcgg cgtcgttgat gacgtgttta ttggtgatga tataaccgcg ttgatccatg
     7021 attacaccgg atcccagggt gcggatctca agctggttgt gagagttggt gttcacacca
     7081 cggttgtaaa cgttaaccac cgctggcgcg gcgcggcgaa ccgccagatt atagctggca
     7141 ggcgtctcat cggtactgtc aaattgcgga gtggaaagcg ggttaaggct gcgcagcgaa
     7201 ggcatggcaa ccagcagaat agcgccgaca attaatccaa tcgcaacgga acgtaagagc
     7261 ttcacaaaca tgatggaggc gtcattaaaa aagggaacgg cagcagcata ccacgagtta
     7321 accggacatc acacgtaagc ctgatgcccg gtttacgaca ttaacgcatc agcagataga
     7381 tgctttcatt gccgcgtaca atttgcaggg cgatgatggc cggttttgcc gccagcactt
     7441 tacgcatttc agcaatcgag ttcacccgat cgcggttgac gccaatgatc acatcgtctt
     7501 tttgcaagcc tgcctgagca gctgggcttc ctttgacaac ttcatcgatc ttaatacctt
     7561 tgccgcaatc ttttagctga ccatcgctca acgttgcacc ttccagcgct ggcgtgatca
     7621 tttcagcgct ggccgacgaa gaggtgctgg tatcaagcgt cacttctact tccagtggtt
     7681 tgccgttacg cagcaggcca agcttcactt tcgtgcccgg ctctgtagtc gcgatacgag
     7741 agcgcaactc agcaaagcta ttcagcggtt tgccgttgag gctggtaata atatcgcccg
     7801 ctttgacgcc cgcttttgcc gagccagaac ctggcaacac ttcgctgaca aacgcgccac
     7861 gctgcacgtc aaggttgaag gctttggcga tatcggcact catctcggtg cctttgatgc
     7921 ctaacaaacc gcgtttgatt tcaccaaagt cgataagctg ctgcgccagt gttcgcgcca
     7981 tattactggg gatggcaaat ccaatcccga cgctcccgcc gccaggcgca aggattgcag
     8041 tgttgatgcc aattaactca ccgttaaggt ttaatagtgc accgccggag ttaccgcggt
     8101 taatggaagc atctgtctgg ataaagtttt ccagaccttc aagattcaac ccgctgcggc
     8161 ctaatgcgga aacaatgcca gaggtggcgg tttgcccaag gccaaatggg ttaccgaccg
     8221 ctacggcaaa atcaccgacg cgcaatttat cggagtcggc aatagcgatt tgcgttaatt
     8281 tgctcgggtt ttgaatttgt aacagggcga tatcgctctg gtcatcgcta ccaatcagtt
     8341 ttgcatcaaa ctcgcgccca tcattgagct gaatactgat tttctgtgcc tgattaatca
     8401 catggttgtt ggtcagcaca tagcctttac tggcgttgat gatgacaccg gaacctaaac
     8461 cttcgaaggg ttgtgcaggt tgatccggta aatcatcacc aaaaaacttt ttgaattctt
     8521 ccgggatttt ctgtccctga ctggccgttc cttccacccg tacgctcacc actgccggaa
     8581 gcactttttc cagcattgga gccagactgg ggagaggggc ctgatcggca acctggcctg
     8641 gaatcgacgc gacggcctga aatgacgccg agagagttaa cccgacactt aacgctaatg
     8701 cactcaacag ctgggtttgt tttttcatta ttcctgctct cgtacctgaa tgataagaaa
     8761 agagattcaa aacagtttga tgttattgaa ttttcagacg gctaacaatg agacaccaga
     8821 ttaaatgata gtcggggagg gagagaagag gagggcgcaa tggctgcgcc cgaaaaataa
     8881 attagtcgcg cttcgcgcca gtacgcagca ggccggatgc accttcagaa tagtcgcgag
     8941 gcatctgcac cggtgcctga tcgttgctgg cttcagactc tgccagacga ttacggaacg
     9001 ggtttgcttc agcagacagt tccggcagca ggctgctgga gcttttcgcc atgtgctgat
     9061 acagctggcg atagtcgtgc gccatggtat ccagtaattc cgcgctgcgg gcaaagtggc
     9121 taaccagctc ttcgcgatac tcgtccagtt cagctttatt cttttccagt tcgtactgca
     9181 acgcctgttg ctggcgtagt ttacgattac caaaacgcat ggccacagca ccaataatga
     9241 tgccgacgac taacccaatt agcgcatatt cccaggtcat gaacatctcc cgttgtcttt
     9301 tgtttccgta gggtgttggc ttcaggctcc atgcctacgg ctgattatgc cactataacc
     9361 gttaattcca cagaagtgga atcccgactg catatcgcgt agtgtagaac ggcctttttt
     9421 tcgtcaaccg tgaacaacgg cgcaccgatt attcaaggaa taataataag atcatgcaaa
     9481 gcgttacccc aacatcgcaa tacctgaagg cgctcaatga aggcagccat caacccgacg
     9541 acgttcaaaa agaggccgtc agccgcctgg aaattattta tcaagaactc atcaatagca
     9601 ggccaccagc ccccaggacg agtgggctaa tggcgcgggt cggtaagctg tggggtaaac
     9661 gcgaagacac aaagcatacg ccagtgcgtg gcttatatat gtggggcggt gtaggacgcg
     9721 ggaaaacctg gctgatggac cttttctatc aaagcctgcc gggagagcgg aaacagcgcc
     9781 tgcactttca ccgttttatg ctgcgggtgc acgaagagct aactgcctta caggggcaga
     9841 ccgatccgct ggaaattatt gccgatcgct ttaaagccga aactgacgtg ctctgttttg
     9901 acgaattttt tgtttctgat attaccgacg ccatgctact tggcggtctg atgaaagccc
     9961 tgttcgcccg cggtattacc ctggtagcaa cgtcaaatat tccgccggac gaactttatc
    10021 gaaatggctt gcaacgtgcg cgttttctgc ctgcaatcga tgccattaaa cagcattgtg
    10081 atgtaatgaa cgtggacgct ggtgttgatt atcgactgcg tacactcact caggcgcatc
    10141 tgtggctttc gccactccac gatgaaaccc gggcacaaat ggataaacta tggttggcgc
    10201 tggcgggagc gaaacgcgaa aattcaccga cgttagaaat caaccatcga ccgctggcga
    10261 caatgggcgc tgagaatcag acgcttgctg tctcttttac caccttgtgt gtcgatgccc
    10321 gcagccagca tgactatatt gcgctctcac gcctctttca tacggtcatg ttgtttgatg
    10381 taccagttat gacgcggttg atggagagcg aagcgcggcg ctttattgcg ctggtggatg
    10441 agttttacga gcgccatgtc aaattagtgg tgagtgcaga agtgccgctg tatgaaattt
    10501 atcagggcga tcggctgaag tttgagttcc agcgttgcct gtcacgtctg caagagatgc
    10561 aaagcgaaga gtatctgaag cgcgagcatt tggcgggtta aaacctgtca caaatcacaa
    10621 aaaggggtcg atctttgacc ccgacttctc tataatcctg cgaccccacg ttacaagaaa
    10681 gttttttccc aaaacttttt gtgtgctggc ataggctatt cgaaggggta ggtttgccgg
    10741 actttgtcgt gtgaacctca acaattgaag acgtttgggt gttcaccaac gtgtaactat
    10801 ttattgggta agcttttaat gaaaactttt acagctaaac cagaaaccgt aaaacgcgac
    10861 tggtatgttg ttgacgcgac cggtaaaact ctgggccgtc tggctactga actggctcgt
    10921 cgcctgcgcg gtaagcacaa agcggaatac actccgcacg tagataccgg tgattacatc
    10981 atcgttctga acgctgacaa agttgctgta accggcaaca agtgtactga caaagtgtac
    11041 tatcaccaca ccggccacat cggtggtatc aaacaagcga cctttgaaga gatgattgct
    11101 cgccgtcctg agcgtgtgat tgaaatcgcg gttaaaggca tgttgccaaa aggcccgctg
    11161 ggtcgtgcta tgttccgtaa actgaaagtt tacgcgggta acgagcacaa ccacgcggca
    11221 cagcaaccgc aagttcttga catctaatcg ggattatagg caatggctga aaatcaatac
    11281 tacggcactg gtcgccgcaa aagttccgca gctcgcgttt tcatcaaacc gggcaacggt
    11341 aaaatcgtaa tcaaccaacg ttctctggaa cagtacttcg gtcgtgaaac tgcccgcatg
    11401 gtagttcgtc agccgctgga actggtcgac atggttgaga aactggacct gtacatcacc
    11461 gttaaaggtg gtggtatctc tggtcaggct ggtgcgatcc gtcacggtat cacccgcgct
    11521 ctgatggaat acgacgagtc cctgcgttct gaactgcgta aagctggctt cgttactcgt
    11581 gacgctcgtc aggttgaacg taagaaagtc ggtctgcgta aagcacgtcg tcgtccgcag
    11641 ttctccaaac gttaattggc ttctgctccg gcagaaaaca attttcgaaa aaacccgctt
    11701 cggcgggttt ttttatagct aaaatctgaa tcagcgtaaa aactggaaag ttgctttttg
    11761 ctgccacctg acagacaggt aaaacaaaaa ccacatcgcc aataagggac taagtcaact
    11821 atttcagact aaagcgcatc tctttttccc catttccggc atcgactcac cacaaaggtc
    11881 gcaaaatctg gtaaactatc atccaatttt ctgcccaaat gtcgggtatt gctcattttt
    11941 tgtttgattt tcgaacaaag agagtcgttc cttactgggt aacacaactt ctgactggcc
    12001 acctggtggc tggtagcagt aaaaattctg actatacctg gaggttttca tggctgtcgc
    12061 tgccaacaaa cgttcggtaa tgacgctgtt ttccggtcct actgacatct atagccatca
    12121 ggtccgcatt gtgctggctg agaaaggtgt aagtttcgag atcgaacacg tggaaaagga
    12181 caatccgcct caggatctga ttgacctcaa cccgaatcag agcgttccga ccctggtgga
    12241 tcgtgagctg accctgtggg aatctcgcat cattatggaa tatctggatg agcgtttccc
    12301 gcatccgcca ctgatgcctg tttacccggt agctcgcggt gaaagccgtc tgtacatgca
    12361 tcgcatcgaa aaagactggt acacgctgat gaacaccatc atcaacggtt cagcttctga
    12421 agcagatgcc gcacgtaagc aactgcgcga agaactgctg gcgattgcgc cggtcttcgg
    12481 tcagaagccg tacttcctga gcgatgagtt cagcctggtc gattgctatc ttgctccgct
    12541 gctgtggcgt ctgccgcaac tgggcatcga gttcagcggc ccgggtgcga aagagctgaa
    12601 aggctatatg acccgcgtct ttgagcgtga ctctttcctt gcttctttaa ctgaagcaga
    12661 acgtgaaatg cgtctgggcc ggagttaatc tgtatggatt tgtcacagct aacaccacgt
    12721 cgtccctatc tgctgcgtgc attctatgag tggttgctgg ataaccagct cacgccgcac
    12781 ctggtggtgg atgtgacgct ccctggcgtg caggttccta tggaatatgc gcgtgacggg
    12841 caaatcgtac tcaacattgc gccgcgtgct gtcggcaatc tggaactggc gaatgatgag
    12901 gtgcgcttta acgcgcgctt tggcggcatt ccgcgtcagg tttctgtgcc gctggctgcc
    12961 gtgctggcta tctacgcccg tgaaaatggc gcaggcacaa tgtttgagcc tgaagctgcc
    13021 tacgatgaag ataccagcat catgaatgat gaagaggcat cggcagacaa cgaaaccgtt
    13081 atgtcggtta ttgatggcga caagccagat cacgatgatg acactcatcc tgacgatgaa
    13141 cctccgcagc caccacgcgg tggtcgaccg gcattacgcg ttgtgaagta atacaaaaac
    13201 aggccctggt ggcctgtttt gtcttttaat ggcttaataa aatagcgtga ttgtggcaat
    13261 catattcacc acgaaaccga ctgccatggg gatcgctgtt cgcttcacca ccgcaaaagg
    13321 cgaaacgcct gcaattcctg acgtaacgac caccaccgca gtaatcggcg aaaccgcgcg
    13381 cgctagcgtc gtggcaaaat gcatcggcat aatcattaca accgctggta catgtagtcc
    13441 agctgcgata ttcggaataa gactggcaaa tgacataaac ggcgcattgc cagagcccat
    13501 cacaatggca caaatggcaa tgaccagcgc cataataatc atcacgccaa taccgcccag
    13561 accagaatgc tccgcacccc tgataaccgc atcgacagtg ccaatcgtcg ttaagccttt
    13621 cgcaaatatt tcacccgcga ccaccagcgt taccacgttg gcaaactgcg tacccatgcc
    13681 gtcaaaaaac gcctgcacat cgtccattgt ttcgcgcaag ttatgcttgc ggaagaactc
    13741 aacaaacatc gtcaccgtca aactcagtaa catcaccacc accagatgca gttctgactg
    13801 catcagcccg acgtgggcga ggaacagcga gccgagcatc aggattaacg gcatcacagg
    13861 taaaatggcg taatagagcg gcgggacatt atcgagagct ttttgctctg cttgttcgtg
    13921 attgatattt ttatcttttt tgtcaaaagc gcgttgcacg aaaaagtggg agatcgccac
    13981 cgagataatg acgcaagagg cgaccggaag ctggtagtgg aagaagtaag tggcaatttt
    14041 cattcccgcg acctgggctg caaaaatgga gttcgtttcc agaattcccc actcaatgga
    14101 cattgtggtt gcgataaccg ccaccgcaga gagacgactt actcccagac tcaccagtgt
    14161 cggaaataag gtgaccatca gcaacatacc cagaccggag gcgctggtaa taaactgcgc
    14221 catgatttga ccgatgacgt aagttgccga cagaataata tacggtgagc gaatgagttt
    14281 taacgggcgg cttaacaagc tcaccatcgc gcgactggcc cctatgcgct ccatgtagcg
    14341 ggcataccgc ccaccgccat aatcgatagc cccaatccgg caatgcggtt ggacagcatg
    14401 cgcaggattt cattataaat atcgacgatc aagtagccgc tgcttttggc gggctcgagt
    14461 acaccaccga accctaacca gacaccgcac atcatcagga agatacctgc ggcagccagc
    14521 accacctgag gtttgtagtt tttcaggatc aaatagccca tcgtaattaa tacgatgaca
    14581 gatataatta tgccgaacat tgttgaactc cgtgtcaaaa gaaaacggtc aatcccataa
    14641 acggcagatt gaaaacaatg atgttatatt ttttgcaagt ctatttatgg tgcggatgtc
    14701 gtgtttttaa ttgtaggtga gataattttt cattaaaaaa tatgcgctta tgattatttt
    14761 gcaagaacgc attcataata ttcataatgc tcgtgaatag tcttataaat aattcaaacg
    14821 ggatgttttt atctgcgtta cattaatttt tcgcaatagt taattattcc gttaattatg
    14881 gtaatgatga ggcacaaaga gaaaaacctg ccattttccc ctactttcaa tcctgtgata
    14941 ggatgtcact gatgatgtta atcacactga ccttacagaa tgggccttat gaacgcattt
    15001 gattcgcaaa ccgaagattc ttcacctgca attggtcgca acttgcgtag ccgcccgctg
    15061 gcgcgtaaaa aactctccga aatggtggaa gaagagctgg aacagatgat ccgccgtcgt
    15121 gaatttggcg aaggtgaaca attaccgtct gaacgcgaac tgatggcgtt ctttaacgtc
    15181 gggcgtcctt cggtgcgtga agcgctggca gcgttaaaac gcaaaggtct ggtgcaaata
    15241 aacaacggcg aacgcgctcg cgtctcgcgt ccttctgcgg acactatcat cggtgagctt
    15301 tccggcatgg cgaaagattt cctttctcat cccggtggga ttgcccattt cgaacaatta
    15361 cgtctgttct ttgaatccag tctggtgcgc tatgcggctg aacatgccac cgatgagcaa
    15421 atcgatttgc tggcaaaagc actggaaatc aacagtcagt cgctggataa caacgcggca
    15481 ttcattcgtt cagacgttga tttccaccgc gtgctggcgg agatccccgg taacccaatc
    15541 ttcatggcga tccacgttgc cctgctcgac tggcttattg ccgcacgccc aacggttacc
    15601 gatcaggcac tgcacgaaca taacaacgtt agttatcaac agcatattgc gatcgttgat
    15661 gcgatccgcc gtcatgatcc tgacgaagcc gatcgtgcgt tgcaatcgca tctcaacagc
    15721 gtctctgcta cctggcacgc tttcggtcag accaccaaca aaaagaaata atgccacttt
    15781 agtgaagcag atcgcattat aagctttctg tatggggtgt tgcttaattg atctggtata
    15841 acaggtataa aggtatatcg tttatcagac aagcatcact tcagaggtat ttatggcaac
    15901 gaatttacgt ggcgtaatgg ctgcactcct gactcctttt gaccaacaac aagcactgga
    15961 taaagcgagt ctgcgtcgcc tggttcagtt caatattcag cagggcatcg acggtttata
    16021 cgtgggtggt tcgaccggcg aggcctttgt acaaagcctt tccgagcgtg aacaggtact
    16081 ggaaatcgtc gccgaagagg cgaaaggtaa gattaaactc atcgcccacg tcggttgcgt
    16141 cagcaccgcc gaaagccaac aacttgcggc atcggctaaa cgttatggct tcgatgccgt
    16201 ctccgccgtc acgccgttct actatccttt cagctttgaa gaacactgcg atcactatcg
    16261 ggcaattatt gattcggcgg atggtttgcc gatggtggtg tacaacattc cagccctgag
    16321 tggggtaaaa ctgaccctgg atcagatcaa cacacttgtt acattgcctg gcgtaggtgc
    16381 gctgaaacag acctctggcg atctctatca gatggagcag atccgtcgtg aacatcctga
    16441 tcttgtgctc tataacggtt acgacgaaat cttcgcctct ggtctgctgg cgggcgctga
    16501 tggtggtatc ggtagtacct acaacatcat gggctggcgc tatcagggga tcgttaaggc
    16561 gctgaaagaa ggcgatatcc agaccgcgca gaaactgcaa actgaatgca ataaagtcat
    16621 tgatttactg atcaaaacgg gcgtattccg cggcctgaaa actgtcctcc attatatgga
    16681 tgtcgtttct gtgccgctgt gccgcaaacc gtttggaccg gtagatgaaa aatatctgcc
    16741 agaactgaag gcgctggccc agcagttgat gcaagagcgc gggtgagttg tttcccctcg
    16801 ctcgccccta ccgggtgagg ggaaataaac gcatctgtac cctacaattt ttataccaaa
    16861 gcgtgtgggc atcgcccacc gcgggagact cacaatgagt actacaaccc agaatatccc
    16921 gtggtatcgc catctcaacc gtgcacaatg gcgcgcattt tccgctgcct ggttgggata
    16981 tctgcttgac ggttttgatt tcgttttaat cgccctggta ctcaccgaag tacagggtga
    17041 attcgggctg acgacggtgc aggcggcaag tctgatctcc gcagccttta tctctcgctg
    17101 gttcggcggc ctgatgctcg gcgctatggg tgaccgctac gggcgtcgtc tggcaatggt
    17161 caccagcatc gttctcttct cggccgggac gctggcctgc ggctttgcgc caggctacat
    17221 caccatgttt atcgcgcgtc tggtcatcgg catggggatg gcgggtgaat acggttccag
    17281 cgccacctat gtcattgaaa gctggccaaa acatctgcgt aacaaagcca gtggttttct
    17341 gatttcaggc ttctctgtgg gggccgtcgt tgcctctcag gtctatagcc tggtggttcc
    17401 ggtctggggc tggcgtgcgc tgttctttat cggcattttg cctatcatct ttgctctctg
    17461 gctgcgtaaa aacatcccgg aagcggaaga ctggaaagag aagcatgcag gtaaagcacc
    17521 agtacgcacg atggtagata ttctctaccg tggtgaacat cgcattgcca atatcgtaat
    17581 gacactggcg gcggctactg cgctgtggtt ttgcttcgcc ggtaacctgc aaaatgccgc
    17641 gatcgtcgct gttcttgggc tgttatgcgc cgcaatcttt atcagcttta tggtgcaaag
    17701 tacgggtaaa cgctggccaa cgggcgtaat gctgatggtg gtcgtgttgt ttgctttcct
    17761 ctactcatgg ccgattcagg cgctgctgcc aacgtatctg aaaaccgatc tggcttataa
    17821 cccgcatact gtcgccaatg tgctgttctt tagcggcttt ggggctgcgg tggggtgctg
    17881 tgtcagcggc ttcctcggtg actggctggg aacccgcaaa gcctacgttt gtagcctgtt
    17941 ggcctcgcag ctgttgatta ttccggtatt tgcgattggc ggcgcaaacg tctgggtgct
    18001 cggtctgtta ctgttcttcc agcaaatgct tggacaaggg atcgccggga tcttaccgaa
    18061 actgattggc ggttatttcg ataccgacca gcgtgcagcg ggcctgggct ttacctacaa
    18121 cgttggcgca ttgggcggcg cactggcccc aatcatcggc gcgttgatcg ctcaacgtct
    18181 ggatctgggt actgcgctgg catcgctatc gttcagtctg acatttgtgg tgatcctgct
    18241 gattggtctg gatatgcctt cccgcgttca gcgttggctg cgcccggaag cattgcgtac
    18301 tcatgacgct atcgacggca aaccatttag cggtgccgtg ccatttggca gcgccaaaaa
    18361 cgatttagtc aaaaccaaaa gttaatcctg ttgcccggtc tctgtaccgg gcctttcgct
    18421 aagggaagat gtatgtcgtt acttgcacaa ctggatcaaa aaatcgctgc taacggtggc
    18481 ctgattgtct cctgccagcc ggttccggac agcccgctcg ataaacccga aatcgtcgct
    18541 gccatggcat tagcggcaga acaggcgggc gcggttgcca ttcgcattga aggtgtggca
    18601 aatctgcaag ccacgcgtgc ggtggtgagc gtgccgatta ttggaattgt gaaacgcgat
    18661 ctggaggatt ctccggtacg catcacggcc tatattgaag atgttgatgc gctggcgcag
    18721 gcgggcgcgg acattatcgc cattgacggc accgaccgcc cgcgtccggt gcctgttgaa
    18781 acgctgctgg cacgtattca ccatcacggt ttactggcga tgaccgactg ctcaacgccg
    18841 gaagacggcc tggcatgcca aaagctggga gccgaaatta ttggcactac gctttctggc
    18901 tataccacgc ctgaaacgcc agaagagccg gatctggcgc tggtgaaaac gttgagcgac
    18961 gccggatgtc gggtgattgc cgaagggcgt tacaacacgc ctgctcaggc ggcagatgcg
    19021 atgcgccacg gcgcgtgggc ggtgacggtc ggttctgcca ttacgcgtct tgaacacatt
    19081 tgtcagtggt acaacacagc gatgaaaaag gcggtgctat gaccacgttg gcgattgata
    19141 tcggcggtac taaacttgcc gccgcgctga ttggcgctga cgggcagatc cgcgatcgcc
    19201 gagaacttcc gacaccagcc agccagactc cgcaggcgtt gcgtgatgcc ttagccgcgt
    19261 tagtcgcccc gttgcaagct catgcgcagc aggttgccat cgcttcaacc ggaattattc
    19321 gcgacggcag cttgctggcg ctgaatccgc ataatcttgg tggattgcta cactttccgt
    19381 tagtcaaaac gctggaacaa ctttccaatt tgccgaccat tgccattaac gacgcgcagg
    19441 ccgcagcatg ggcagagtat caggcgctgg aaggcgatat aaccgatatg gtgtttatca
    19501 ccgtttccac tggcgtcggc ggcggtgtag tgagcggcgg taaactgctt accggccctg
    19561 gcggtctggc ggggcacatc gggcatacgc ttgccgatcc acacggccca gtctgcggct
    19621 gtggacgcac aggttgcgtg gaagcgattg cttctggtcg cggtattgca gcggcagcgc
    19681 aaggtgaact catgggcgcg gatgcgagaa ctattttcac acgcgccggg cagggcgacg
    19741 agcaggcgca gcaactgatt caccgctccg cacatgtgct ggcaaggctg atcgctgata
    19801 ttaaagccac aactgattgc cagtgcgtgg tggtcggtgg cagcgttggt ctggcagaag
    19861 ggtatctggc gttggtggaa acgtatctgg cgcaggagcc agcggcattt catgttgatt
    19921 tactggcggc gcactaccgc cacgatgcag gtttacttgg ggctgcgctg ttggcccagg
    19981 gagaaaaatt atgatgatgg gtgaagtaca gtcattaccg tctgctgggt tacatcctgc
    20041 gttacaggac gcgttaacgc tggcattagc tgccagaccg caagaaaaag cgcctggtcg
    20101 ttacgaatta cagggcgaca atatctttat gaatgtcatg acgtttaaca ctcaatcgcc
    20161 cgtcgagaaa aaagcggaat tgcacgagca atacattgat atccagctgt tattaaacgg
    20221 tgaggaacgg attctgtttg gcatggcagg cactgcgcgc cagtgt
//
LOCUS       DAJVTY010000064        19988 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVTY010000064 DAJVTY010000000
VERSION     DAJVTY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.064"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..572
                     /locus_tag="OQQ66_002082"
     CDS             <1..572
                     /locus_tag="OQQ66_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCS1905015.1"
                     /translation="FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVE
                     VNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFP
                     IVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTC
                     SVVTYSQLPELKQPKLRGNVQTAQREVAP"
     gene            569..1658
                     /locus_tag="OQQ66_002083"
                     /pseudo
     CDS             569..1658
                     /locus_tag="OQQ66_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(1746..3044)
                     /gene="exuT"
                     /locus_tag="OQQ66_002084"
     CDS             complement(1746..3044)
                     /gene="exuT"
                     /locus_tag="OQQ66_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1905016.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3527..4939
                     /gene="uxaC"
                     /locus_tag="OQQ66_002085"
     CDS             3527..4939
                     /gene="uxaC"
                     /locus_tag="OQQ66_002085"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1905017.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDTAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            4954..6441
                     /gene="uxaA"
                     /locus_tag="OQQ66_002086"
                     /pseudo
     CDS             4954..6441
                     /gene="uxaA"
                     /locus_tag="OQQ66_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6524..7075
                     /locus_tag="OQQ66_002087"
     CDS             6524..7075
                     /locus_tag="OQQ66_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1905018.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7080..8324)
                     /gene="sstT"
                     /locus_tag="OQQ66_002088"
     CDS             complement(7080..8324)
                     /gene="sstT"
                     /locus_tag="OQQ66_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1905019.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(8940..9905)
                     /gene="alx"
                     /locus_tag="OQQ66_002089"
     CDS             complement(8940..9905)
                     /gene="alx"
                     /locus_tag="OQQ66_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1905020.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10189..11175)
                     /locus_tag="OQQ66_002090"
     CDS             complement(10189..11175)
                     /locus_tag="OQQ66_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1905021.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11254..11937)
                     /locus_tag="OQQ66_002091"
     CDS             complement(11254..11937)
                     /locus_tag="OQQ66_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1905022.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12014..12517)
                     /locus_tag="OQQ66_002092"
     CDS             complement(12014..12517)
                     /locus_tag="OQQ66_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1905023.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12602..13738
                     /gene="rlmG"
                     /locus_tag="OQQ66_002093"
     CDS             12602..13738
                     /gene="rlmG"
                     /locus_tag="OQQ66_002093"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1905024.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14023..14337
                     /gene="higB"
                     /locus_tag="OQQ66_002094"
     CDS             14023..14337
                     /gene="higB"
                     /locus_tag="OQQ66_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1905025.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14334..14750
                     /gene="higA"
                     /locus_tag="OQQ66_002095"
     CDS             14334..14750
                     /gene="higA"
                     /locus_tag="OQQ66_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1905026.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14795..16813)
                     /gene="fadH"
                     /locus_tag="OQQ66_002096"
     CDS             complement(14795..16813)
                     /gene="fadH"
                     /locus_tag="OQQ66_002096"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1905027.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17039..19391)
                     /gene="ygjK"
                     /locus_tag="OQQ66_002097"
                     /pseudo
     CDS             complement(17039..19391)
                     /gene="ygjK"
                     /locus_tag="OQQ66_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19408..>19988)
                     /gene="ygjJ"
                     /locus_tag="OQQ66_002098"
     CDS             complement(19408..>19988)
                     /gene="ygjJ"
                     /locus_tag="OQQ66_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCS1905028.1"
                     /translation="TRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRY
                     DDKYTYPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRA
                     DGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGG
                     GRFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         4882 a         5245 c         5236 g         4625 t
ORIGIN      
        1 gcttctctga acaggatgaa gagagtcagg atctgcaact cgaagcgcgg gtggcaggca
       61 gccgaacttt gcagcttggt caaagcgaca gtgtgttgtt cccttatcct ggatttcagt
      121 ctggttttgt tgaggttaac gacagtagcc agggcaatca gcaggggaca acaaacatca
      181 ttaacggtgc agggaatcgt gaattaatgt tgttgcctgg caagctgcgc tatcgcgaag
      241 tgtctgccag ctttaactac aactatatcg gtcgtttatt actgccggca gcggtgaaaa
      301 aattcccgat tgtggggctg aacagcgcca tgttactggt agctgaagat ggcggattta
      361 cacttgaaat taacggcagc gaaaaagagc tctatctgct ttccgggcag caattcctta
      421 agtgtccact gagtgttgaa aagaaacgcg ccagcattcg ttacagcgga gatgttactt
      481 gtagtgtggt gacttattca caattaccgg agttgaaaca gcctaaatta cgtggaaacg
      541 ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg
      601 cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg aaattacgta
      661 tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt cggcaggaaa
      721 taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct gtacagctaa
      781 tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga tgcgttggcc
      841 ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc cgctaggatg
      901 caaggggggg caaagggctt cagacgaatc ttaaggattc taatagatct tcatgtacag
      961 aagatggtca acattattat atatacgata caaagtttct tacgctctac cttgagcaga
     1021 cagagatgaa gaatttgccg attggtggcg tctggaaggg gaaagttaaa ttacattcga
     1081 acagcccggc ccaggactat ttcgcaaata ttaccctgaa tacgctcgac cccaaccata
     1141 ttgacgtgtt cttcccggag ttcgcccacg ccacgccgag ggtgcagtta gacttgcatc
     1201 caacaggaag cgttaacggc agcaactacg cgcaagatct gaccatgttg gacatgtgcc
     1261 tgtacgatgg ttttaacggt aatgccatca gttatgaaat catgctcaaa gatgaagggc
     1321 gaccagctgc agggcgcaga gacggtgact tctctatcta tcgtcaggga ggaacctcta
     1381 ccgacgaggg agaacgcatt gattaccggg tcaaaatgta caacccggaa accggtgggc
     1441 aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg aaaagtgtgc
     1501 gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca cccttggctc
     1561 tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg ggcaaattgt
     1621 cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaagcg gacaaacgct
     1681 tttttctata taaacgacga agccgcctga taaggcggct tcgtcgttat aacttacgtt
     1741 aaacgttaat gttgcggtgc gggatcgtta tgtgattcct gcgctacctc aatcgccggt
     1801 ttgttctgca acacggtcca gataaccagc gcacctaaca ggtcgaacac tgccagtact
     1861 gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag cgaaaacagc
     1921 gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac ttcgttacga
     1981 ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg agcaaaacca
     2041 ccgatacaca gcagcataat tgcgacatac gggttggtga acaggccgat catacccggg
     2101 ccaatcatca gcactgcacc cagcgttacg accatcttac gggaaacgat caggttcaca
     2161 ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc gaggtcagca
     2221 aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc gtaaacttta
     2281 aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ctggttctgc caggaaacgc
     2341 ggcagcgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga cattttcttc
     2401 gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg ttcttcatcg
     2461 gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat cgcccagata
     2521 aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcattac gattgcccat
     2581 accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa gtaacctacc
     2641 gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc cgccgggatc
     2701 atcgctgctt ccgcggcacc gactgcacca cgagcaatcg ccaggccacc cctgctacca
     2761 gccagcgcgg ttgcaccaca gaatacggcc cacagtacag caaacattgc ataaccgatt
     2821 ttcgtaccca gcacatccag tacatagccc gctaccggtt gcatgaccgt ataagcagca
     2881 gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc ttccatcaga
     2941 gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt gccaagcgtc
     3001 accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa cctcatcgtt
     3061 ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat gttagttacg
     3121 accgggtaac cgaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt ttcgtgctaa
     3181 ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg gcaatttgca
     3241 ttccgtcgac ttatgatttg cgacggcaga aagatgactt gtcatacaac tttaaaaggt
     3301 gagagccatc ataaatgtgc gaatatttgt agggacatta cctgacgaca gcgaggccag
     3361 tactggcgcg gcctgcagcg agatttacca ctttgagagt aattttttta actacgttta
     3421 ttgatctaac tcacgaaaat atcttcggac tctggaaatt ggtgtgataa ctttgtcagc
     3481 atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga ctccgtttat
     3541 gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg actacgcaaa
     3601 agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg cggaagacta
     3661 tcgttttaaa aacctgtatg acatctggct gaaaggcgat cactacaaat ggcgcgctat
     3721 gcgtaccaac ggtgtggccg agcgtctgtg taccggtgat gcgtctgacc gtgaaaaatt
     3781 tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc actggacgca
     3841 cctcgaactg cgtcgtccgt ttggtatcac tggcaaattg ctttctccgt caactgccga
     3901 tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg cgcgcggcat
     3961 catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg attctctgga
     4021 gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc cgagctggcg
     4081 tccggacaca gccttcaaca tcgaacaggc gacctttaac gactacatgg cgaagctggg
     4141 cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct tgaccaaacg
     4201 tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg acgtggtgat
     4261 gtttgctgaa gcgaacgaag cggaactgga cagcattctg gcgcgccgtc tggctggcga
     4321 aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt tccttggtgc
     4381 cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc gtaataacaa
     4441 cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca acgaccgtcc
     4501 gatggcagaa gagttgtcta agctgctgag caagcagaac gaagaaaacc tgctgccgaa
     4561 aaccattctg tactgcctga acccgcgcga taacgaagtg ttgggcacca tgatcggtaa
     4621 cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt ggtttaacga
     4681 tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtt tgctgagccg
     4741 ctttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc acgaatactt
     4801 ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag caccggcgga
     4861 catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc gtgactactt
     4921 cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat ccatgcgctg
     4981 gataacgtcg cggtcgcttt agcggatttg gctgaaggca cagaagtcag tgtcgataac
     5041 cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt aacggatatc
     5101 gcaaaagggg ccaatgtcat caaatatggc ctgccgattg gttatgcatt ggcggatatt
     5161 gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga tctggatcag
     5221 tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcg tgaagtgcag
     5281 atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat cctgccaacc
     5341 gtgggctgtg tcaacggcat cgcgcggcag atccagaacc gttttctgaa agagaccaac
     5401 aacgctgaag gtaccgacgg cgtgttcctc ttcagccaca cctacggctg ctcacagctg
     5461 ggcgacgatc acattaatac ccgcaccatg ctgcaaaaca tggtgcgcca cccgaacgca
     5521 ggcgcagtgc tggtgattgg tctgggctgt gaaaacaact aggttgccgc attccgtgaa
     5581 acgctgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca gcaggatgat
     5641 gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg caacgataaa
     5701 cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg ttctgacggt
     5761 ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt gattgctaac
     5821 ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca gttgctgatg
     5881 gaccattgcc gcgacgaagc aacgtttgaa aaactggtca ccatggtcaa cgactttaaa
     5941 cagtacttta ttgcccatga tcagccgatc tacgaaaacc catcaccggg gaacaaagcg
     6001 ggcggtatca ccacgctgga agacaaatca cttggctgta cccagaaagc gggttccagt
     6061 gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccagggct gaacttgtta
     6121 agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg ctgccatatg
     6181 gtgctgttca gtactggtcg cggcacgcca tacggtggat ttgtgccgac ggtgaaaatc
     6241 gccaccaaca gtgaactggc ggcgaagaaa aaacactggc tcgactttga cgcgggtcag
     6301 ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacat catcgttgag
     6361 tttgccaacg gtaaacaaac ctgcaacgag cgtaacgact tccgcgaact ggcgatcttc
     6421 aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg cgtttcataa
     6481 tgtgaagcgc cgtttgcttt tccatacctg ataaggattt atcatgaccg cgtattggct
     6541 ggcccagggc gtgggtttca tcgcctttct gattggtatc acaacatttt tcaatcgtga
     6601 cgaccgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg tacatttttt
     6661 tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc gtacattgat
     6721 taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga ctggcggaat
     6781 tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta cgattgtcag
     6841 taccagggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt ggttttcaac
     6901 gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacaa tgattgaggg
     6961 tagttttctg cttatgaatg gcttgaatat cattcgtttc tggcggatgc agaaaagggg
     7021 aattgatccg tttaaagtag agaaaacccc tcccgccgta gacgaaaggg gttaaacaat
     7081 taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc tgccgcagtg
     7141 aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa tacgccgatg
     7201 ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa catattacag
     7261 gccagcggga tcagcagcag agaacccccc gccacgccgg atgcgccaca ggcacacaga
     7321 gaagccacca cgctcaacag cagcgccgtg ggcagatcga ccgggatacc cagcgtatta
     7381 accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt gatggtggca
     7441 cccagcggaa tagaaacgga ataggtatcg cgatccagat tcagcttttc acacagtgcc
     7501 atattcaccg gaatgttcgc cgcagagctg cgggtgaaga aggcatacac gccgctttcg
     7561 cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca caccagcaat
     7621 gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag cagttgcgcg
     7681 tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag accaaaaata
     7741 ccaatcggtg cgaagcgaat gaccagtttc accataaagg tgacggcatt cgacagatcg
     7801 ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaaaccgag gccaattgcc
     7861 cacaccagga tcccgatgta gttacctttc agcagcgcgt cgatggggtt ggaaaccatg
     7921 ctcattacca gcccgcgcat cacttctaca atgcctgacg gtggcgaaat atcacccgcg
     7981 ctactggata agtgcagggt agaagggaag gcgaagctga aaacgactgc ggccagagca
     8041 gcagagaagg tacccagcag atagaggaac aaaatagggc ggatattggt tttctgcccg
     8101 tgctggtggt tagcaataga tgccatcacc agcatcaaca ccaggatggg ggcaacggct
     8161 ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc gggttttgag
     8221 atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac caggctgcca
     8281 tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg atcctttcat
     8341 tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg taacaaagtg
     8401 tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg ctggatttta
     8461 aagagaggtc atattttgca acttaattgt tacatttgtt tgacataaac agagggaagg
     8521 actggctact aaggctgatt agataacata tgggcaaaac tatttggaat atgagaacgg
     8581 ttgcgctcct ttaatgtgct atgccatata aagtcactgg cgcagggagc gcgcaggggg
     8641 cagccaatcg ccgccgcccc ctgcaccccc aggctctggc gaacaaaatc gccgctgcgc
     8701 gattccctca gcttatccct tgcggctacc gggtcgggcg caagggaacg tccctgtaag
     8761 atgcgccctc gccgcgcatc catgcgcgtc gccccggcct ccagggaacg cttcggcgat
     8821 tttgacgcca ccaacacccg tgcggtcttt ttgtaaagag ctacaagcca attacataat
     8881 taatagctca cctttgtctg gaatgagtta tctctaggaa tggcttaata tacagcgaat
     8941 cattctcccc gctgcttatc atgccgataa ttcaccctgg cgttgataat aaacgtcatc
     9001 accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca
     9061 atcagcatct tgataccgat aaacaccagg atcaccgcca ggccatattt gagcatcgag
     9121 aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gatcgcaaac
     9181 aggtttgagg tcagcacaat gaacgggtca gtggtcacgg cgaagatagc cggaatgcta
     9241 tccacggcga aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg
     9301 gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg
     9361 cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca
     9421 tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc
     9481 cagtcgaact gcgaaattag ccagctgcca gtgaagatca tgatggtacg cagaacaatc
     9541 gccccgagca cgccatacac cagcacgcgg cgttgtaatg ccgccggaac agagaaatag
     9601 ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc aatcagataa
     9661 cccgtgagaa aggccagtgc ctgtggatcg gcaacggcgc gaccttcggt ttgcaccaga
     9721 taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccaaaccag cgaccaggcc
     9781 gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata
     9841 gccagcataa tggcgacaac aacagcgaat ccgccccata gcaacggcgt gccgacagta
     9901 ttcatagaag ttccttacac ataaaaaaaa cggccaacgt cggaagacgt cagccgctgc
     9961 tttttatgca tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt
    10021 ctgcgtattc ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac
    10081 gatcgaccgg caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag
    10141 ccagtcaatg gtctgttaca taccttttac actgttttac tttggcaatt atagttttac
    10201 gctatctgcc ggaaaaatca ccccagtctg gcggcggatc tcagtcagca gtttggcggt
    10261 gatgcgactg accgccagcc ctggatgatc caccagatgc tcatccacca gggtagcaaa
    10321 cagctctgct tcgtagagca tggtattaat atgctgcggc tgggtgagat cctgcatctg
    10381 gctgccacgc ggcacgaagc acactttctg gcattcagac agtttttcaa tcaccagcga
    10441 tcccgcttcg ccctgaatct cgctttccag gacagaatca ctgacctttg agtgcagcaa
    10501 cgtaacgctg aaatcaccgt aatccatcac caccacgccg tgggcatcta cgccgcttgc
    10561 caacaaactg gcggttgcct gcacgctttt cggttcaccg aataatgcca ccgccgacgc
    10621 caggcagtaa aagccgatat ccataattga accgttagag aatgccggat taaaggtgtt
    10681 gggattctca ccatccaggt aacgttgata ccgcgaggaa tactggcaat agttgaaaaa
    10741 gactttacgc aatttgccga ctttcggcag cgcctggcgc agcaaatgaa agttcggcag
    10801 gctggcggtt ttaaatgcct caaacagcac cacctgattt tcccgcgcac aggcaatggc
    10861 ggcatccact tccgccagat tcgacgccag tggtttctcg caaatcacat gaattttatg
    10921 gctaaggaaa agttgtgtct gggaaaagtg cagggcattc gggctggcaa tatacaccgc
    10981 gtcaatggca tcgctttccg ccatcgcttc cagcgaggta aacagatgct cgacagaaaa
    11041 atcattggcg aagtgctggg cctgttcaag gctgcgggaa tatacggcgg ttaacttgta
    11101 tttaccgctc tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg taccaatcac
    11161 agcgaaacgt atcataacgc gtggatactc cataaaggga ttctgtcgtc aatgtagcac
    11221 gcctttttca tcttcgacgt gcgcagatac gcattattct tttgccggac agccatgctc
    11281 actaaacggc ccaatcatga ccgatggccc gagatatttc ggctggctat ggaggatttt
    11341 ggcgtcaatc acggcgctga cacgagcgag tttctctcgc agttgagtat aaaaaccgtg
    11401 cttcatattg aggtcaggcg cgttaaaacc gatagcgtcg atgctgtact gttttgccag
    11461 ccagatggcg cgttggttat gaaattcttg cgagataatg gtgatatggt tttcaccaaa
    11521 aacctttttg gcacgcacta ccgaatccag cgttgagaat ccggcatagt cgcagaagat
    11581 cactttcgcc ggtacgcctt tagcgatcaa agccttctgc atacctgatg cttcatcgta
    11641 atttttacgt ccgttatcgc cacttaccag tagccatttc acttttccgg cgtggtacaa
    11701 cgctgctgcg gtatcaatac gtcgggtaaa gtagcgatta cccggcctgg cccccagcaa
    11761 taagcccacg ttacgcgccg gaactgcgtt gacatcgctc caggtcagct gcttgctggc
    11821 gttgaccatt actcgatcgg cgataaaaat ggtcgctcct gccacaagta acagtaagca
    11881 ggcaatcttt agagtccggc gagaaaaaca aatacggaga agaaggcggg gctgcatggt
    11941 cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga aaagataacg
    12001 cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc cacagccggg
    12061 tgtcgaactc cagctggtgg tactgcggct ccatatggca gcacaactgg taaaacgctt
    12121 tgttatgctc tttctccttc aggtgcgcca gttcgtgcac cacgatcata cgtaaaaacg
    12181 gttccggcgc gttgcgaaac acagtagcga cgcggatctc tgcttttgct ttcagcttgc
    12241 cgccctgcac acgagaaaca gcggtatgta acccgagcgc gttttttagc acatggatct
    12301 tattgtcata catcacttta ttgatcggcg gggcattgcg cagaaactga ttttttagat
    12361 cctgagtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc ggatagcgtt
    12421 ttgccagcac atcgcccaga cgctgttcgt taatcagcgt tcgcacctgg gaaagtagct
    12481 gctcgggata accctgaaga taagtaagat tgctcataac cgcccacgaa aaagagaaaa
    12541 agggtatact cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt caggagggcc
    12601 gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc cggcgacgga
    12661 tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc aacagttgga
    12721 cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg cgttaagctg
    12781 cgcgctggcg gaacataagc cgtacagcat tggcgactca tacatcagtg aactggcgac
    12841 gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc tcgacagcac
    12901 cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa cactggcatt
    12961 gctggaacaa caactgcgtg cgctgcgcaa agtggtcacg tcggatacac gtattattgc
    13021 cggtgccaaa gcccgtgaca ttcacacttc cacgctggaa ctgttcgaaa aagtgctcgg
    13081 cccgaccacc accacgctgg catggaaaaa agcgcgcctg attaattgca cgttcaatga
    13141 accgccgctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta ctgactggac
    13201 tatccataac catgcgaatg tcttctcccg caccgggctt gatatcggcg cgcgcttctt
    13261 tatgcaacat ctgccagaga atctcgaagg ggagattgtc gatctcggtt gcggtaatgg
    13321 cgttattggt ctgacgctgc ttgataaaaa tccgcaggcg aaagtggtgt ttgtcgatga
    13381 atcgccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc cagaggcgtt
    13441 ggatcgcagc gagtttatga tcaacaacgc gctctccggc gtggagcctt tccgctttaa
    13501 tgctgtgctc tgcaacccgc cgtttcacca acaacatgcg ctgaccgata acgtcgcctg
    13561 ggagatgttc caccatgccc gccgctgcct gaaaatcaac ggcgagctgt atattgttgc
    13621 caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact gcaccaccat
    13681 cgccacgaat aataaatttg tggtgctgaa agcagtgaag ctggggcgtc gtcggtaaga
    13741 ttgggagtta cctgccggat gcggcgttaa cgccttatcc ggcctacgtt cagcacgtta
    13801 ctcccaggcc ggataagacg cggcaagcgt cgcatccggc aatgtctgcc cagttctgga
    13861 atcgcgcttc cataaaacaa aaaatccctc tcaccatcct ctacattctc ttgtttagcg
    13921 tttttctacg tttattcttc cgtcacacag ataaattcat ccgttgccaa tctgtcaacg
    13981 gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct gataactcaa
    14041 aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt ggtggctctg
    14101 gggaacatga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc agtattccca
    14161 tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg gggcaatgaa
    14221 ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg tgaagttatg
    14281 acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa aaaatgattg
    14341 cgattgccga catcttgcaa gcaggagaaa agctaactgc ggtggtacct tttctggcgg
    14401 gtattcagaa cgaggaacaa tacacccagg cgctggaact ggtagatcat ctgctgctca
    14461 acgatcctga aaaccccttg ctggatctgg tgtgtgccaa aataaccgcg tgggaagaat
    14521 cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc ggtatagccg
    14581 tgattcgtac ccttatggat caatatggtt taacccttgc cgatctgccg gaaattggca
    14641 gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg gaacacgcta
    14701 aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa cgcgacgtgc
    14761 cggatgcgtt gcatccggcg cttcaaataa cgatttaaat ctccagcgcc agccgtgttc
    14821 cctgggcaat tgcccgtcgt gcgttcagct ccatagccac atcgcagccg ccaattaaat
    14881 gcacagtttt cccgctatca atcagcggtt gcgccagcgc gcggtttggc tcttgccctg
    14941 cgcagatcac cacattgtcc acagctaata cctgcgtttc gccgttgatc accacatgca
    15001 gcccgtcatc gtcaatcttc tgataactta cgcctgggat cattttcaca ccccgcgaga
    15061 gcagggtggt gcgatggatc cagccggtgg ttttgcctaa cccctgtccc ggtttgctgg
    15121 ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg gatctgcatt ccctgcgggc
    15181 ttaagccacc agcctgttgt aggctgctgt cgatccccca ctcattacag aacctggcga
    15241 tattctggct ggtggattcg cccggctgac ttaaatacat cgccgtatca aaaccaatcc
    15301 cgccacaacc gatgatggca accttgttgc ctaccggcgc tttgtcgcgc agtacatcga
    15361 gataactcaa taccttcgga tgatcgatcc cgtcgatagg cggaacgcgc ggcacgatcc
    15421 cactggcgag gatcgtttca tcgaacgcct gtaactgatc cgccgtcacg gtgtgattga
    15481 gtttcagcgt cacgcccgtc acttcgatca tccggtggta atagcgcagc gtttcgtaaa
    15541 actcctcttt gccggggatc tgtttggcga tattaaactg cccgccgctc tcgctgtggg
    15601 catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt aatggcaaac gccagcccag
    15661 caggtcccgc accgaccacc gccagatttt ttttctgcac ggcaggaagg attggcattt
    15721 tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt gactttgcca acgaagatct
    15781 gatcgaggca ggcctgattg cagccaatgc aggtgttgat ctcatcggct cgccccgatt
    15841 gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg tgccatcgat accatatcgg
    15901 catcgccgcg cgagagaata tcgtcggcaa cctgcggatc gttaatccgg ttggtggtta
    15961 ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac ccagctaaat gcgccgcgcg
    16021 gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca gccaatgccg gtgttgataa
    16081 tggtcgcgcc cgccgcttca atggcctgcg ccagctctac cgtttcggca aaagtcccgc
    16141 cgtcttcgac caggtcgagc atcgacagtc ggtagataat aatgaagtcg ttgccgacgc
    16201 gttcgcgcac cgcacgcact acttctacgg caaatcgcat ccggttgcgg taatcgccgc
    16261 cccactggtc actacgctga ttggtgtgca gcgtcagaaa ttcgttgatc aaataccctt
    16321 cggaccccat tacctctaca ccgtcgtatc ctgcctcccg tgccagttgc gcgcagtggg
    16381 cgaaatcgtc gatcagttgc aggatctctt catgggtgag ttcgtgagga acgaaacggt
    16441 tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg ttggtagctg tagcgcccgg
    16501 tatgcaaaat ttgcagggct attttgccgc cttcctgatg taccgcttcg gtaatggtgc
    16561 gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc accttccatg ccaacgcctg
    16621 ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac gccgtgacgg gcgcgttcgg
    16681 cataaaacgc tgccagccgc tcggcaccgt ccgggtattc ctccagcccg gtgtgcattg
    16741 agcccatcaa cacgcggttt tttaacgtgg taaaacctaa atccagcggg gcgaacagcg
    16801 acgggtagct cataaaattg tccagtatgt aaaataattg ttatgtggtc ggatgagttc
    16861 taatttagcc gtcgccggag aaaagggaaa aggggagtgc ggggtttgtg atgggattca
    16921 aaaagaatac gcccggtggt aacgccggat gcgacgcttg ccgtgtttta tccggccagg
    16981 aggattgcac atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt
    17041 attgcttacg gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat
    17101 ttggtgcagc cttgctgcgc gccagtcagc gggttgtaat tctcctgaat tgggccatcg
    17161 gcggttaatc ctttggcgtg ccggaagaac gtatccgcca gcttcagggc atcatcacga
    17221 taaccgtaac gctccatccc tttcagagca aaccagaact gatccaccca tacgcgcccg
    17281 cgccagtaga tatcagcgcc aaaagccgga ttggttaacg ccgccgttcc cagcgggaca
    17341 aaggtgttga actctttagg atcgagcatc accttcacca ccgcgtcggc attggcctgc
    17401 gttgccgcac cgttaaacag cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc
    17461 ggtttgcccg cgcagccgtt cgccagtggt ttatcttcaa tacgtacgtc atagtagaac
    17521 tgggtagtcg ggtcgaacat acaggtgttg atgtagtccg cgagctgctg tgccaactgg
    17581 cgatagcgtt tggcctcttc tggcttaccg aggatggttg ccatctccgc cagataatgg
    17641 ttatcgctgt acatatagct ggcctgatcc accgactcct gcaatagcga gtagcccagc
    17701 aacgttccgt cctgactgcg gttttcggcg aatttcaccg tccagtcgct acgtttgcca
    17761 ccattagcga catatttatc caactgttct ttgtcgataa aaccaaagac ggcggcatca
    17821 tcacgccccg attcccacga cgcagcgacc tgtgccggaa tttccagact gtcgtactgg
    17881 cctttctcca ccacgcgggc gtagttgttc agcccagact gcgtctcttc tttgttgcct
    17941 ttcttcaccg caaacagcat ctcgccgctc tcagtgttgt gggctttgtc gcgggtcgcg
    18001 ccatattccg gcacgccgtt gccgttgtga tcgcggttac gtaaccacca gtcgtgatag
    18061 gcgaccagtt tcgggtacat ctctgccaac caggctttat cctgggtgac gttgtacact
    18121 tctatcaccg accaggcggc aaggctgggt ttggtattac gttcgttcca gttgccgcca
    18181 tcgccaccac gctcggggct aaggttccac gctatcaggt cggggacaaa gcccacatcc
    18241 tgcggacgca cgcggtcgcc aggctggatc tgccaggaga agaccgcgcg gatattctct
    18301 ttggcgatgt ccggattgaa atgcgccatc gcaaacgcct gcttctaggt atcccacggc
    18361 caggtctgat tgccggagaa ccagcgcccg gtcaccgacg gtgtaacggt gttaaatttc
    18421 accgcaccgc ccggtgagcg ccagttaccg ttgagcgttt cgatcgcttt caccgcgacg
    18481 cgcgtctgtt ccggcgtcgc atccggattg gttaaccctt tcttcaggta ttcttcccag
    18541 cgttgctgcg aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt
    18601 tgctctttgc taacttcctg agcggtcagc agatgggaat aggtggtgta gagcgtggtc
    18661 gaaccgttga tatgcgcctt actggtaaag cgattaccgt tgatttcagt ctgcaccggc
    18721 agggatttat gcacctgata ttccgattcg ccggaggtca gcagatccca ggtggcgcgc
    18781 actttgccaa aggtgacttt caggccatca cgggtggcgc tgattttgcg ctgatagtca
    18841 gggtattcgc cagcaatggt tttatcggaa agcggtttcc cttctttcgc ttccagtttt
    18901 tccagcagtt cgccgtccca caccagatcc agcggtttat tgctggtgat tttggtttcc
    18961 agtagtgacg tgcgcggcgt ggcgaagcgc agagtcattt cgacttgcac atcttttgct
    19021 gtcagttttt gcaccagcgc accgggaata ctgtatgcct ccagcgtaaa gtcgactttc
    19081 ttgccgtcct gccagacggt caggcggtcg aaattgctgg ccataaagtt gatgtactct
    19141 tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt tagggccgtc tggcaacaga
    19201 tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg
    19261 taatccttca tgtactgcgg cgcgccagta cggttaatca cgtttttata attgtcggcg
    19321 ttagcggcat gggcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt
    19381 tttattttca tgaaattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg
    19441 gtcgctggga tcgtgagagg catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa
    19501 acgtccgccg ccgtattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc
    19561 agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccctttaa
    19621 cgcttcatac atggcgctca ccatcatccc actttcactt cccggtacgt ccagcccgtc
    19681 ggcgcgtccg gtatggtgcc aggcgcgagc ggcaaagtta ggagttagca ggcccgtgag
    19741 atagagctgt cctgtgccgt cggtgatttc cagaccgttc atccaggtga tgccttcggc
    19801 aaggtcatac tggatgtaac cgttcatcat tgccggatag gtgtatttgt cgtcatagcg
    19861 atcgtatttg ccaaaatgta gccaggcttt gctttcatca tgatgtccgg caggagtcgc
    19921 ggtgacgcta taacgaaagt tgccgcttaa gttctgtacc ttcagcgcag tcataatgtc
    19981 gcgggtat
//
LOCUS       DAJVTY010000065        19877 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVTY010000065 DAJVTY010000000
VERSION     DAJVTY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19877
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.065"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..4205)
                     /gene="mukB"
                     /locus_tag="OQQ66_002099"
     CDS             complement(<1..4205)
                     /gene="mukB"
                     /locus_tag="OQQ66_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1905029.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRL"
     gene            complement(4205..4909)
                     /gene="mukE"
                     /locus_tag="OQQ66_002100"
     CDS             complement(4205..4909)
                     /gene="mukE"
                     /locus_tag="OQQ66_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1905030.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(4890..6212)
                     /gene="mukF"
                     /locus_tag="OQQ66_002101"
     CDS             complement(4890..6212)
                     /gene="mukF"
                     /locus_tag="OQQ66_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1905031.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6209..6994)
                     /gene="cmoM"
                     /locus_tag="OQQ66_002102"
     CDS             complement(6209..6994)
                     /gene="cmoM"
                     /locus_tag="OQQ66_002102"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1905032.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7130..7909
                     /gene="elyC"
                     /locus_tag="OQQ66_002103"
     CDS             7130..7909
                     /gene="elyC"
                     /locus_tag="OQQ66_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1905033.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7886..8779)
                     /locus_tag="OQQ66_002104"
     CDS             complement(7886..8779)
                     /locus_tag="OQQ66_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1905034.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIYGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8933..9679)
                     /gene="kdsB"
                     /locus_tag="OQQ66_002105"
     CDS             complement(8933..9679)
                     /gene="kdsB"
                     /locus_tag="OQQ66_002105"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1905035.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9676..9858)
                     /gene="ycaR"
                     /locus_tag="OQQ66_002106"
     CDS             complement(9676..9858)
                     /gene="ycaR"
                     /locus_tag="OQQ66_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1905036.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9910..11142)
                     /locus_tag="OQQ66_002107"
     CDS             complement(9910..11142)
                     /locus_tag="OQQ66_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1905037.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11179..12165)
                     /gene="lpxK"
                     /locus_tag="OQQ66_002108"
     CDS             complement(11179..12165)
                     /gene="lpxK"
                     /locus_tag="OQQ66_002108"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1905038.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAEQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12162..13910)
                     /gene="msbA"
                     /locus_tag="OQQ66_002109"
     CDS             complement(12162..13910)
                     /gene="msbA"
                     /locus_tag="OQQ66_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1905039.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13947..16219)
                     /locus_tag="OQQ66_002110"
                     /pseudo
     CDS             complement(13947..16219)
                     /locus_tag="OQQ66_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16426..16710)
                     /gene="ihfB"
                     /locus_tag="OQQ66_002111"
     CDS             complement(16426..16710)
                     /gene="ihfB"
                     /locus_tag="OQQ66_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1905040.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16870..18543)
                     /gene="rpsA"
                     /locus_tag="OQQ66_002112"
     CDS             complement(16870..18543)
                     /gene="rpsA"
                     /locus_tag="OQQ66_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1905041.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18654..19337)
                     /gene="cmk"
                     /locus_tag="OQQ66_002113"
     CDS             complement(18654..19337)
                     /gene="cmk"
                     /locus_tag="OQQ66_002113"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1905042.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19510..19877)
                     /gene="ycaL"
                     /locus_tag="OQQ66_002114"
                     /pseudo
     CDS             complement(19510..19877)
                     /gene="ycaL"
                     /locus_tag="OQQ66_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4775 a         5519 c         4843 g         4740 t
ORIGIN      
        1 agacggcaag gagagatatc tttaccgcgc aggcggcgag attcatcttc ccagctttgt
       61 accaccatca ccagaatcga cataccggta ccaatcgcct caccggtcga caatgcacca
      121 gactctgcgc gcaaccaacc atcggaacca cggttaacct caacttccat ttccagatag
      181 ttgcggtaat ccagcagttc ttcgccaatg gtctgcggcg tgcgctgccc catatcaatc
      241 tgcggattaa gacgctgata caatttcgcc aacgcttccg agaaggtcag acggttgctg
      301 ttaaacagat cctgatgctg ctcgtgctgt tcagagagca catccagcag catggcgtgc
      361 gtttcacgca cgttcacgtt gaggcgcacg ctgttcacct gaccaaacga tacgttctgc
      421 aacccctggt tgagcatacg gatacggttc tgctcgcgct gaatggtttt gcgaatgatg
      481 ttcgccacgc tgcgggaact gatcaccagt ttctgttcac gggaggttaa ttcttcggtc
      541 agacggctaa gttcgatctc catctgttcg atagcttcca ccggatcatc ggtacgaata
      601 atatcctgac gaatacgttc acgcagatgc tgataagccg ccacgaagaa ctgaatttta
      661 cgctccggac gtttcggatc ttccgacatg cgcagcacgt cgcgcagatg ttcgttatcc
      721 gccaccgcca gacgcagcgc acccaatgcc ttatccgaca tagagcgcaa atcatcagcg
      781 gagagataag ccagctcacg acggtgtaag cggcgctcaa cgccgttatc tttcaccatg
      841 cgcatcaccg cacaccagcc cgctttggcg gtcactacct gctcgcgcat ctcaaagtaa
      901 tcccgctcca gcttgcgcag tttgcgggtc aggttgtcca tctccgcttc gcagaaggta
      961 agcgcttttt ccagttgatt gcggcgtgaa cggttattgc tcagttgcgc atgcagctcg
     1021 tcacggcgaa tacgcgcccg ctcttctgcc ccgctatcag cacacacgcc gatatcctgc
     1081 aattcacgtt gcagatcgtt gagtagctct tttttggtgt cgtaagaact tttcagcgaa
     1141 gccagcacct ggttgtactg actcaactgc gctgcgtgac cgcgcaacgc ttcgcgagcg
     1201 cgggtacgct ccgcttccgc ctgttccaga cgttcacgca gtttttcgtt gagatcgctg
     1261 ttaccgctaa gcatttctgc cgagtcagag tagctaaagt gcgcacgacg ctgcaccact
     1321 tccgtcaggg caaacgcctg ctgacgggca tcgcgctgca tctgttgaga gtacgcgtaa
     1381 tcttctttta actgttcgaa ctgttccggg tcgctctgca ataccgaaac gatcggttcc
     1441 agtttcgcca gttgattgcc aaactgctga acaaaacgcg cggcttcctg ggcttcatcc
     1501 agacgttcgc ggatttcatc gacgcgatcc gccaggctat catcagccaa caggttgaga
     1561 cgcggcagaa tgcggttcag cgccgtaacg ccctctttcg cctgctcaaa ctgaatacgc
     1621 tgctgctggt tatcattttc atgattactt aacgcccgct ccagttcgac gcgacggctg
     1681 ttcagttgac ggatttctgc ttccgggtca gactcaaaca caaccgccag atgactgccg
     1741 ataaagcggc tgaacgcctg atgcagacgc tgagttttct gtacatcaaa ggagagcgtg
     1801 gcgaagcgtt cggaaagcac ttcacgctcg gcatggaggc tttcaatacg gctttcacgc
     1861 gcagcacgac caaacagcgg cacttccggg aaacgtgaat aacgccactg acgatcggcg
     1921 attttcacca ctaccgcttt ttccagctca tcaacgctga acacgctgtc atcgaatgac
     1981 tgcggatctc cttcgatcag atagagatct tccgggcaat cggtcaagcc atctaggtgt
     2041 tcagttacct gtgacagatc tggcaccacg atggcgtggc gtgacgggcc atacagcgct
     2101 gagaagtacg gcgcatcttc caggctaacg tcgtcataaa tttctgacag cagcacacca
     2161 ccaaaacgct ccgccagcgc gttcagacgc tgatcttcag agccgccagg ctggcttaaa
     2221 cgttcgatct cttcatcgac ggcgtttttg cgcgcgccca cttcatcgcg ttcaacaatc
     2281 gcctctcgct cacgctccag caactgttgc agatattcgg tgacgtcctg gctggaggta
     2341 aactcttcgc cgcactgttc gctcaactgg ttgagactgt tttgcgctgc cagccaaacc
     2401 ggcgcacgct gcatcaaact ctgaatgcga gactgcagct gttcctgctc ctggcgcagt
     2461 gccatgcgct cttcacgggc gttagacacg ctatcggaaa gagaggcaat gcgtgcttcc
     2521 agttcctgat gcagggcttc cagttcgtcg atatcaaaat tcttgccctg acgtttgcag
     2581 aaatctgcca gcagacgctc agcttcttgc tgctcgcgta gacgctgttc cagttcgctt
     2641 aatcgcatcc gcagcggctg aacctgctct gccaggtgac gctgatcgac cccttcgcgc
     2701 aatagttcgc gagcgacatc ccacgcctcg ttacgcgcca gtgggccgtt gattgccacc
     2761 accagctgat aagcctgctc aaactggctg tgcgcggttt gcgccatgct cattttctgc
     2821 tcaagagaga gcattttttc agtcgcttcc agctctttcg cctggaaggt ttccagccat
     2881 tcggcggcgc tgtcggcggt taagtccggc agatggcaca gttctttggc acgattaagc
     2941 gcagcaatcg cctggttata ctggatcgcg cgcgtttgct ggacgtccag cgcctgctgg
     3001 tagtcagcaa gctggctttt cagctcgtcc acttccagtt cggcagcttc cgcgcgagcc
     3061 tcgttctctt cctggcgttc gatggcttct gccaccactt cgttttgctc ttccagacgg
     3121 atctgcagct catcgagatc cgcttcgtag cgttcgatct tctcctgctg acgcagtgcg
     3181 gtttgcacca ggttcaggtg atcgctggcc gcctgataat ccgcttccag atcgccttcg
     3241 gcaccgttgt gctctgccag ctcacgcgcc atatcgacgt gtttgtactg ctcagccgcc
     3301 agttgctgac gcgaagtatg taactcgcga cgaaactcca gggctttgtc gagatggaca
     3361 cggcgctcgt tggcgtgacg catgtagtcc gccgccacgt agttggtagc ttcgctgatc
     3421 agatgcttaa acaggtcacg gtccgactgg gtgacacgaa tcgcttccag cgtcatgcgg
     3481 ttttcacgca gcgccgcttc catgtcctgg aacgctttac gcacgccgct gttttccggc
     3541 aacagatagt cgcgcagaga acgggtaatg gcactggaga tcccgccata cagcgaagct
     3601 tcgatcagac gatagaattt gctacggtca gatgcggagc gcagacgacg cgcgatgatg
     3661 cccagatcga acatcagcga gtggtaatcg gtaatggagt tgaactgttt aaactgcacg
     3721 ccttccatcg cctcgagctt gtctttcagc tcgttaagcg gcagcacgcg cgcctggcgt
     3781 tcgttcaggg tttcggtcac cagctgtgtc ggctgcaccg acatcggcag tccctgaatg
     3841 gcaaatggtt tgatatccac tttacgatcg cgtccggcaa cctgttgcag acgcacaccg
     3901 accaccacgc gctggtggcg cgagttaatg gtgtcgagca tcgaataaca gacacccgct
     3961 ttcagcttac cgtgcagacc tttatcgcgc gaaccgctgg tggccccggc ttccgtagtg
     4021 ttacggaaat gcagcagggt caggtcgggg atcagcgccg taacgaacgc cgccatggtg
     4081 gtggatttac ccgccccgtt accgccagaa agcgtcgtga ccagctcgtc aaggtcaaaa
     4141 gttcgggcaa aaaagccgtt ccagttaatc agcgtcagtg agcgaaattt accgcgttca
     4201 atcattattc ttcctctccg ctatctggct gattctcttc ggtttcatcg ttgagttgca
     4261 gatgattttc aatcggcatt gcttcgccat cacgaatcag gcgacgctgt gcttcacggg
     4321 gatcgtcgcc agcacgcaca tcggctccga agcggaacac cgattcggta atgcgaaact
     4381 tgctgctgtc gtggcccata aaccacacca tgcctaaacg acgcagacgg ttgagcgaag
     4441 aacgtacttt ctcctgcaac ttctgacggt caacgtctga accggttgaa cggttgttca
     4501 ccagtttcag cagttttgct tcatcggcca gggtgagcag ttcgtcgtac agttcctgct
     4561 gggtgaaaat cccctcattc gccagccgtt ccgggctgag atagagataa cagaggattt
     4621 tcccgaccat catatccagt tccgacaaga cggaacgcgg gataagagtg gtggaacgtg
     4681 ggcgtaaata gaagaaccct tccggtgcgc gaataagctc aacgttataa cgcgcgtaaa
     4741 actcttccag atattcctga aaatccatca ggaatgcatg attatccagt tcgtcgaggc
     4801 caatatggcg gcctgaacgt aaggcgctgt ccagcgccgg aaataacgga ttcgccagcg
     4861 cctgcgccag cttaaccggc atcacttgtt caatatttgt cgatgacatg cgcctgtacc
     4921 ttggctccgt aatcattaat cggctgccat ttcgctggca gtccggtgaa atctgcttgc
     4981 gctacgccaa gacgtaccgc ctgatcaata acaatacgcg caacgtcaaa gtgacgtgca
     5041 cgcggatact gtgacagata ttcgcgtacc accagaccaa gatccagcgg cacttgtctg
     5101 gttttgtaca cggcaagttg ttcttcgatg atcgccgcca gctgttcgcg gatctcgtta
     5161 aactcttcgt attccagatc ctcaggaagt tccccagtca cttcttcatc gcgcagtgcc
     5221 atctcttcgt cacgcatatc cagcagacga tcggcattgg cataagttag cgcccacggc
     5281 tcatcaaaat aggtttgtac cgactgacgt aaccgctgag caaagacgcg gtttttatcc
     5341 atatcgatcg cggtacgaat aaatttgtgt acgtggcggt cgtagccaat ccacaagtcg
     5401 atggattgct ggccccaact gataatacga tcgagtttgc tctgcagatc gaacaccaga
     5461 cgatcgacga aatgcagatc gtcatgggtc atcgtcgcat cctgaatgcg caacagatta
     5521 gcctgcaatt tgtcgcctgc cgcttccagc gtatcctgca attcacgcag cgttccggaa
     5581 gtttccgaaa gcaacaattc acagctggaa atcgccgccc gccagtcttt gttcagcaac
     5641 tgggcgatat cgtccttcac ctgctgctgc tgttcgtcca tcagacgttg cgtcaggtcg
     5701 atactgtcga aaatttctgc taccgaatat ttcagtggcg catagacatt acggtgccag
     5761 tgaaattcat caccgccctc ttcggcggca tctgctgcgc gtttgagctc acccgccaca
     5821 atcgacaact gcatagaaag acgcagcgta gaaaactcgc gctgacggat gtagtagtca
     5881 gtaatgccga tgccgagcgg cgtcagacgg taaattgcgt tcccttccgc ctgctcgctg
     5941 gtaaagcggt tcagcagacg ttgacgcacc atgtcgttga tcgcgttatt ggcgcgcacg
     6001 ccgatggttt cgctggtttg ctcaaacgca tcactcacat ggcggaatgc atccaccagc
     6061 tcgccttcac tcatctcacc atccagacgc tcgccgttca gcgtggcaac cgccagcaga
     6121 aaagagagtc ggtctacctg cagcgagatg gagaagtcat tttttctggc ccaggcaacc
     6181 agttcgggga ctgtctggga aaattcactc atactttatc cttgctctgc ggtttgcgcg
     6241 cggtgacatg aatataacgc cccagggtaa tatacggttc ctgacggcaa taacgcgttt
     6301 ctaattcaag taatgcttca tagcagtcgc gctgctggtg cttctcgcgc agataatcat
     6361 gaaacacgcg aacgcctgtc ttacccataa tttgccaacc agcttcttcc agccacagat
     6421 aaacctgcgc cgggtcgcgt ggataatctg gcgaaagcgt ccgttttttc tttttcggca
     6481 ttcccgcctg cacgtaatca aaattcccgg cgaccatgtt atgcatcaac aaaccatgcg
     6541 cattgtagaa cattaacgac aacacgccgc ctggacgcaa cactgaccac agggtctgca
     6601 atacactgcg gggatcggcc acccactcga gcaccgcatg gaacaatatc agatcaacgg
     6661 gcgtttccaa atgcgaagca acatcctgag cggcgcaatg tataaattgc atgttgtcgc
     6721 tcacaccttt tgcttctgcc gcctgttttg cgcggtcgat catctgcgca gaaagatcgc
     6781 ataaaatgac ctgatgccca cgctcggcca ttttgattgc ggtctgccct tctccaccgc
     6841 cagcatccag cacacgcagt ttttgcgggc ccatttccgc cagcacgcga tcgagatcct
     6901 gccacagaat agcctgtcga agctgccctt tggtggtgcc gtaaatgtta cgggaaaact
     6961 tttccgcaat atcatcaaaa ttgcgatcct gcattgcttt ctccaccggt tggctaagac
     7021 gttttctgat aaagacgcta ttttgtcaca gccacggcga gaatgaaccc gtctggtgta
     7081 atatcgctgg cgatcccttg ctatatggtt aaaaaaggaa ccagaaagga tgctttttac
     7141 actgaaaaaa gtgattggca atatgttgct gccacttccg ctgatgttgt taattattgg
     7201 cgctggcctg gcgctgctgt ggtttagccg ttttcaaaaa accgcgaaga tttttattag
     7261 cattgggtgg ctggcactct tgttattgag tttgcaaccg gtagccgacc gcctgctgcg
     7321 tcccatcgaa agcacctatc cgacatggaa taattcgcaa aaagtggact atatcgtggt
     7381 gctcggaggt ggttatacct ggaacccgca gtgggcaccg agctctaatt taatcaataa
     7441 cagtctgcca cgcctgaatg aaggtattcg cttatggcgc gaaaatccgg gatcaaaact
     7501 gatcttcacg ggaggcgtag caaaaaccaa tacggtgagt acagcggaag taggtgccag
     7561 agttgcgcaa tcgctgggcg tgccgcgcga gcaaattatc accctggatt tgccaaaaga
     7621 taccgaagaa gaagctgcgg cagtgaaaca ggcgattggt gacgcccctt tcctgctggt
     7681 gacctccgca tcacatctgc cgcgggcgat gatctttttt cagcaggaag ggttaaatcc
     7741 tctaccagcc ccggcgaatc agttggccat cgactcgccg cttaatccgt gggaacgggc
     7801 aatcccttct ccggtctggc tgatgcatag cgatcgtgtt ggctatgaaa cgcttggccg
     7861 catctggcag tggctgaaag gtccgtcagg cgagccacgg caagagtgat tttgatgcca
     7921 gatcgaagtt gcgccgacta aatcgtccgg tattaaccag ttgcgcaact tcatcccata
     7981 acacatacaa ccaacgccgc cagatgaacg actccgccac tggcgcgcgt tgcaggtaac
     8041 tccagagcaa atcttccgcc agagaattat ccattagtcg gaacagttcg tattctcgcg
     8101 gtgcccaaag cattagtccc ggcccgacca tcgccagtaa ctgatcgctg cgcgaatctt
     8161 tcagcatgct gcgtaaacag aagttaccgt aaatcaacac acaattgtcg ttaaagcctt
     8221 caaataatgc cgggagacat tcgcgagtgc gaaacaggat ccgcttatcc tgcatcgtca
     8281 gaccggtgtt attgaactga ttgagcgtgg tccatagcac ttcaacatgt tgccggtacc
     8341 atgagggcca gaaattttcc tgagtattgt cgaccgcgcc gacgcaaccg cgactgtcct
     8401 gacggtgcca tgccagtaag gcttcaacga tttggtcttt gagctgttcc cagcgttctg
     8461 gtgttcgggc tggtgcctcc accgaaacac cacgcatccg ctccagtagc aggacatcag
     8521 ggccggggtg ttcttcatgc gtcatcacac cataaatagt cggcatacgg acagtcccac
     8581 ttcttgccag catggtggtt ttccatgcca gttgtcgggc ttttccgggc gtactaaagc
     8641 ttcttgccat taacggcatt gggtttccct ggctgtcata caaagcccac aacgccgtat
     8701 ccgctttttc attgacgcac tcaatacgac tgagtttttc gcccagtaaa tggcttaatt
     8761 cggcacgcag ctgttccatt cagattaccc tcataaaagc tactgctttc ataatgagcg
     8821 ccaatataaa agatgtcacc tgaatgagat caattgttga aaagaaaaat tgaggaaaat
     8881 cgtaacggta cgacactcct cccaaaattg gctgaagtgt cgtgaagtga aattagcgca
     8941 tttcagcgcg aacgcgctca agatcttcag gggtatccac acctgtgcca ggaacttcct
     9001 gagcaacagc aacatggatt ttttcgccgt accacagaac acgaagctgc tctaacattt
     9061 cgatgtgttc taacggactt gcctgccagt tgacgtaacg acggataaag cctgcacggt
     9121 agccataaat gccaagatga cgcaggaagt tatcgccaac ggtttcaagg ccttctgcaa
     9181 aacgatcacg atcccaagga atggtggcgc gtgagaagta cagtgcatac ccttcagcgt
     9241 cgagaaccac tttcaccgca ttcgggttaa acgcttcttc cgcattgtgg attggcaccg
     9301 ccagagtcgc catacccacc tgacgctgag cgaggttatc agcaacctga cgaatgattg
     9361 tcgcagggat catcggttca tcaccctgca cattaacgat caccgtgtcg tcgctgaatg
     9421 cgcatttttc gacaacttcc gccagacgtt ctgttcctga ctgatgatcg gcgcgcgtca
     9481 tacatacttc accgccagcg gcttcaacgg cgcgggcaac atcctcatga tcggttgcca
     9541 cgatgatgcg ctcggcacct gattcacgcg cgcgttcaag aacatgaaca atcatgggtt
     9601 tgccgttaat atcaaccaat ggtttaccgg gcagacgcgt ggacgcgtag cgcgcgggaa
     9661 taatgaccac aaaactcatg atttactctc atcagcagtc agcacgcggg cttccgtttc
     9721 cagcaacact ggaatgccat cgcggagcgg gaaagccagg ttatccagtt tgcaaataag
     9781 ctcttgtttt tcctggttat accaaagttt tccgttgcaa accgggcagg caatgatttc
     9841 aagcaaacga tgatccatag ttcctccaga tggatcgggt tatgaatgca taaatcttat
     9901 cataatcatt tatgcgacgg ggtctatttc ccagccagtg cggcaatcag taaagagacc
     9961 ttgcgggcag cgtcctaatg tcacccgcgt tgcctgctgc cagctcgcga aatcagtaat
    10021 cgcctgacgt aacccttttt gcagcattgt cgttggttta atgccttcct gtaaccacag
    10081 agagataact tcaaggatgc ctgtctggcg atgcattttg gcatccattc gcccaactaa
    10141 ttgcccacga tgtaataatg gcagaacaaa atagccatac tggcgtttcg gcgctggggt
    10201 atagcactcc agccggtagc taaaatcaaa aagctgctct gcgcgtttgc gatcccagac
    10261 aacaggatca aaaggcgaaa gtaccgcgct gtgcgttgca gtgagctttc cggccagcgc
    10321 acgctcgagt agcggcagca aatcagcatg cagccaaaga ttgcccaatt tttcaacatg
    10381 cacagcaatg atttgctgct gttcagccct cgcttcgcgc cacgccgcca gtgccggacg
    10441 tttcagccga tagtaatctg ccagccactg ttcgcggaat attcccaggc tacgcgcact
    10501 gttatccagc atgatgattt ctgcttctgt ttgcgaaacg agatcgcgct catcatccca
    10561 gtcaggcatg acacggtggg ttaaatcata aacgcgctgg aagttgcgcc gttcaatcac
    10621 catcaccttt ccggcagtaa ataaaccttc cagatgccgt ttatgcggtt tccattccca
    10681 ccagccgctt gcacctttac gaggatgctc aaaatcggct gaacgtaccg gccccttatc
    10741 atgaatatgc tgaattaact gtgcaatttc cgcctcatgt tcctgcatcc aggcgtcttt
    10801 gtatttccag cccatttttt caggtgccag catgcggtgg cgaataagac gaaagtcgct
    10861 acgcggcata aagcaggctt catgcgccca gtattccatt aattcgccac gcgccagaga
    10921 ctcatccagc cactgggcag gataatttcc cagacgactg aaaagcacca gatatggact
    10981 acgggcaaca atattgatgg tatcgatttg cagcaaggac atgcgggaga tcgttgccgg
    11041 aatatcctcc aacgacgctc gacggcgggg tttgtttaac aggccttgtg cggcaaggtg
    11101 aagattacgc gcatcagcaa gggagaggtg cggcagcgac attcatgact ccatcaatcg
    11161 aacgctgccg cggcgtaact agttgccaga agccagcgag gttagttgcg taagcagttt
    11221 cgctggttca tcacctgaaa gctgtgcgtc tacaggcaaa taccaccaat tttcttctgc
    11281 aaaggcccgg catttcaccg catctttttc agtcattacc agcgtttgct cggcgcttac
    11341 caacgcactg acatccgcat ggttcaaaga ctgatgatcg gccagcggta cacatttttc
    11401 cggttgtacg ccacacatct tcagcgtggc aaaaaagcgc ggcggatgcc caatccccgc
    11461 catcgccact acatgttcaa gctgagcaac gtcacaacgc gtaccggtac gtaaattcac
    11521 cgcctgaccc ggcagcagat gcatgggaat ttcaccgctg cgagggacac cgccgttgac
    11581 gattaccgca tcaaccgact ttaagcgccc cgctcgctca cgcattggcc ccgccggcaa
    11641 ccaccagcca ttgccaaagc gacgcacacc atcaataacg acaatttcca catcacgcgc
    11701 cagacggtaa tgctgtaaac cgtcgtcggt tacgatgatc tgcacatcag ggtgttgcgc
    11761 cagaatggct tttaccgcat cagaacgaac gggagaaacc gcaacaggcg catcagtgcg
    11821 ttgataaatc aacacaggtt catcacccgc ctgtgctgtt gtggtatctg ccgacaataa
    11881 cagcggataa gattcagcct tactaccata tccccgcgat acgaccccca cgcgaatacc
    11941 gcgctgttgc aactgttcca ccagccagac aacgaccggg gtttttccgt tgccgcctgc
    12001 ggtgagatta ccaaccacga caaccggtac gggggcacgc caggcgcgct tcagttttag
    12061 tttatagcaa agacggatcg cgccactcac caggccatac aaccaggaga gtggcagcaa
    12121 tagccgccac aaaggggatt caccagacca gattttttcg atcattggcc aaactgcatt
    12181 ttgtgaagtt gcgcgtaaac gccgcggtgc tcaagcaaat cgttatgcgt accgcgttcc
    12241 acaatgacac catcctcgac gaccacgatt tcgtctgcct tttcaatggt agacaagcgg
    12301 tgggcaatca ccagagaggt acggtttttc tgcaactcat ccagtgccgc ctgaatcgca
    12361 cgttcggatt cggtatccag agccgaggta gcttcatcca gaatcagaat cgggctatca
    12421 cgcagcaagg ctcgagcgat agcaatacgc tgacgctgac cgccagagag cagcacgccg
    12481 ttttcaccaa tcactgtatc gagaccgtta tccatcttat tgatgaagtc catggcgtag
    12541 gccatacgcg ccgcttcttc aatttgctca cggctgtact gttcagtccg tgcgtaagca
    12601 atgttgttag caaccgtatc gttaaacaga tggacattct gcgacaccag agcaacctgg
    12661 ttacgtaacg acgccagggt atactcgcgc agatcgtgac catccatcag gatttcgcct
    12721 tcatcaatat cgtaaaaacg agtgatcagg ctggcgatgg ttgatttacc tgaaccagag
    12781 cgtccaacca gagcaaccgt cttccctgcc ggaattttca ggttgatgtt acgcaatgca
    12841 ggtacgtcac gtcccggata agtaaaggtg acattgcgga attccacgtc gccagtcgca
    12901 cgctcgatca cgcgcttacc ttcgtctttc tcctgctcac tgtccagaat ggtaaacagc
    12961 gtctgacaag ccgccatacc gcgctggaac tgggcgttaa cgttggtcag tgatttcagc
    13021 ggacgcatca gtgcaatcat tgaagagaaa acaacggtaa tcgtaccagc agtcaggcta
    13081 tccatgacac ttgggaagct cgccgcatac agaacaaacg ccagcgccaa agaggcgatc
    13141 agctgaatga tcggatcaga gatggaagag gctgaaacca ttttcatccc ctgaagacgc
    13201 attcggttgc tgactttatc aaagcgtttc gtttccactt cctgaccacc gaaaatcaat
    13261 acttctttgt ggcccttcag catttgttcg gcgctggtgg tcacctgccc catggtgttc
    13321 tgcatgtttt tactgatgtt gcgaaaacgc ttcgatacta cgcgaatcgc aatcgaaaca
    13381 atcggtgcca gcacaatcaa aatgatcgac agttgccaac tgtaatagaa catcatgatg
    13441 aacaggccga tgattgacgc accttcacgc acaaccgtta tcagtgcgcc ggaagaagaa
    13501 gaagcaacct gttcggaatc gtaggtaata cgtgacaaca gcgtccccgt tgactgtttg
    13561 tcaaaaaatg aaaccggcat tcccatcatg tgaccaaaca ggcggcgacg catggtcatt
    13621 accacctttc ctgataccca ggagatacag tagctggaga catagctggt gataccacgt
    13681 aaaatcatca gcccgatcac caccagcggc atccacacca gcacggagcg atctgtttta
    13741 ccaaagccat catcaagaag tggcttaagg agcgataaca tgaaggtatc gctggctgcg
    13801 ttgaggatta acgctacgcc cgccacgatc agacccgctt taaaaggcgc aatggttggc
    13861 cacagtcggc ggaacgtctg ccacgtagag agatctttgt cgttatgcat tcaaaaaacc
    13921 agcatttgtt gaaatagccg catattctac ccgttatcca ctggcacgcc aaaccactga
    13981 tgataccaac gaggtaaaat ttgctctctg aggctgctaa tccgccagcc ttgcgctgaa
    14041 aaattgaccg tcacttgacc ctgatgtgga gtatcaagcc attgatatcc ttgctgttga
    14101 tagcgatgct taactttatt agagggcaat cgccatgcgt tatagcgtta tagcgcgatg
    14161 ccgatgcgag tgccactttt ccattcactc gctgaattaa tggcaacgat gatgaggtat
    14221 tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac ttgctgccag taacggctta
    14281 gcatcttttg ttcagcgggg acttcaatat caccggttag aagaatgcta ttcgtcccgt
    14341 cataaacctt aaccacacag gaatggttat ttcctttatc gttgctagct tgtaaaggcc
    14401 agtgcacgct gaaacgcaat ccttgccatt gccacgcttc gccacgcaca cagggctgat
    14461 gatgttccca gtttaacgga cttctgatcc ataacatcgg ccatgtgtgc aatattgagt
    14521 ccagccctcc ccggtgatcc agatgttcat gactcagaat aacgccttcc ggttcaagat
    14581 tatgccagtg gagccagggg ataatcagtt gttgcccact atccccttcg ggccaggcca
    14641 gtcctgtgtc atagagaatc gctttgccgt ttctggctat caccattgcc agcccttgcc
    14701 cgacatcaag catgtacacc tgccactcgt caggtcgagg tttttgccac agcggccagc
    14761 acatcagcaa gcctacagcc acacacattg ctggcaacgt tcgccaggcg tttaatcgcc
    14821 ataccaccag taagaaccat ggggaaaatg atagccattg ccaacgttca gcaatgttga
    14881 tccacccttc ttgcaaactc tttaaccccc agaaaagtaa agccaaagac cggtcggcaa
    14941 gaaaccataa cccttgctcc aggattaacg gcccgcttaa atgcacaacc atcgcggcga
    15001 ggatcaacgg aaccgtgata aatgtcacca agggaattgc taatagattt gcaataaacg
    15061 aggtcagact aatgccatga aatatgacga tttgcacagg cataagcagg agtgtgattc
    15121 ccagttgcaa atgaatgagg gaaacaactg cacgcaatac cggcggcagt tgccactcag
    15181 gacagggaaa ccactggtac caaaaaatca gtgccgcgac cgcagcggca gagagccata
    15241 aactttgcga gagaatggca acaggatcca tcagcaaaat tgccgccaga caacatatcc
    15301 atacatccca gccactccac tgtcgcccac ttaacttaag cattccccac gtagcaagcg
    15361 ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa ccaggcataa aaagcagcac
    15421 agcagattcc accaattaat ggcatttgcc agtggatcca gcgcccaggc agaaaaactt
    15481 gcccaccgcg aatgagtccg gcagccagca acgccgcaaa agcgatgtgc aatcccgata
    15541 tcgccattaa atgcgccgtt ccagtatcac gcatgatatt tttgatttct ttggggacgg
    15601 ataaccgttc ccccatacct aaaccaagaa taaccgcatt ccacatatag ggttgcagcg
    15661 ttgtttgtag tgacgccaga tactgtgcac gcaggctaca attcggttca atgacacttg
    15721 cctgcagaaa gcggccgatg agcggctgat gctgggcaat ggcataacgc tggctatcaa
    15781 agccgccatc attaagttgg ccatgaactg cacgaacttt gagtttcatt gaccattgtt
    15841 gtccagcaca aaccgcttgc ggaagatatt cgccatacag cacaagacct ggcgcaggga
    15901 atatacgtcg accttgtaga tgagtaattt gaccgtaatg agtggtcatg ccgtcagttg
    15961 cagtgatctc aacaattgca tcctgcgtcg cgccagttaa ggtttctcct gcccacaaaa
    16021 tttgctttgc tgataatatg ccccaaacga aaaacagcaa cgtcagcgcg atataacgga
    16081 cggttttaac agggataaat gccagtacgc aggcgaagag agtcagaaac gcaagggtta
    16141 atgtcccagg caattggggc aaaatcagca acggaaaaat tccgcaaatt atgcatacgc
    16201 cgactgtcgt tattttcatg cacccctccc tgtttatgga aagtgtgcaa ctttgttgag
    16261 cggttgtctg gtgggaattt tgcgttcccc gaggcacatt cagaagaatt taacgaaagg
    16321 gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc ctgattagag aaatgaaaaa
    16381 agcacccgac aggtgctttt ctcgcgttca agtttgagta aaaacttaac cgtaaatatt
    16441 ggcgcgatcg cgcagttctt taccaggttt aaagtgagga acgtattttc cttccagttc
    16501 tactttatcg ccagtcttcg gattacgtcc ggtacgtggt gcgcggtagt gcaaagagaa
    16561 actgccgaaa ccgcggattt caatacgctc gccctgcgca agagtcgagg ccatatgctc
    16621 cagcatctct tttactgcat cttcaaccgt cttggcggga atgtgcgatt gctgggtggc
    16681 aagtctttct atcaattctg acttggtcat gattcctccg gttccttaaa ggcaaattaa
    16741 gcggctgctg ttgctgcatt gattaagggc ggccgtagcc gcccttagtg cttgattaca
    16801 ggacgaaacc tgcaatctgt caagtaaact caacaaactt cggaataaaa atcccgaaga
    16861 gtcagagaat tactcgcctt tagctgcttt gaaagcttca gccattgcgt tgttggagaa
    16921 gtttgcatct tcctgtttgt taacagttgc gattgcatct ttctcgtcag cttcgtcttt
    16981 cgcacgaaca gacaggctga ttgcgcggtt tttacgatca acgccggtga atttagcttc
    17041 aacttcgtcg ccaacgctca gaaccagggt agcgtcttca acgcggtcac gggatgcttc
    17101 agaagcacgc aggtaacctt caacgccgtc agccagttct acggttgcgc ctttagcgtc
    17161 aactgcagtt actttaccgg ttacgatagc gcctttcttg ttcagagcaa cccagttgtt
    17221 gaacggatct tctgcgagct gtttaacgcc caaggagata cgttcacgtt ctgcgtcaac
    17281 ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat tcacgaactg cttcttcgcc
    17341 tgcaacgttc caggagatgt cagacaggtg aaccaggccg tcgatgccgc cgtctaggcc
    17401 gatgaagata ccgaagtcag tgatagactt gattttacct tcaacacggt cgcccttgtt
    17461 gtgggtttcc gcgaactgct gccacgggtt agctttgcac tgtttcagac ccagggagat
    17521 acgacgacgt tcttcgtcga tatccagaac cataacttcc actacatctc caacgttaac
    17581 aactttggac gggtggatgt ttttgttggt ccaatccatt tcggaaacgt gtaccaggcc
    17641 ttcaacgcct tcttcgattt caacgaagca gccgtagtcg gtcaggttgg tcacgcgacc
    17701 agtcagtttg gtaccttccg gataacgttt agcgatagct acccacggat cttcgcccaa
    17761 ctgtttcagg cccagggata cacgggtacg ttcgcggtcg aacttcagca ctttaacagt
    17821 gatttcgtcg cccacgttga cgatttcgct cggatgctta acgcgtttcc aggccatgtc
    17881 agtgatgtgc agcaggccgt caacgccgcc cagatcaacg aatgcaccgt agtcagtgag
    17941 gttcttaacg atacctttaa cttccatgcc ttcctgcagg ttttccagca gctgatcgcg
    18001 ctctgcgctg ttttcggatt cgataacggc acgacgagaa acaacaacgt tgttgcgctt
    18061 ctgatccagc ttgattactt taaattcaag ctctttgcct tccaggtgca gagtgtcacg
    18121 caccggacga acgtctacca gagaacctgg caggaacgca cgaataccgt tcagctcaac
    18181 agtgaagccg cccttaactt tgccgttgat aacaccggta acagtttcag catcttcgta
    18241 agctttttcc agcgtgatcc aggcttcgtg acgtttagct ttctcacggg acagcagagt
    18301 ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca acttcgtcac ctacctggat
    18361 ttccagctcg ccctgggcgt ttttgaactg ctcagccggg atggcggact cagatttcag
    18421 accagcgtca accagtacta cgtctttgtc gatagcaaca acaacgccac gaacgataga
    18481 acccgggcgg gtttcgattt cttttaagga ctcttcaaag agttgagcaa aagattcagt
    18541 catgtttaat cttcaggttt atatttaacg tccacctggc tccatgccgg atggggttgt
    18601 ttaacatacc cgctgtcatt ccattgcaac gggggtactg caaattcggt cgcttatgcg
    18661 agagccaatt tctggcgcgc gtattgtagc gctttttcaa tcacttgctc aatgcttaag
    18721 gtggtggaat ccaacactaa agcatcggct gccggaacca gtggcgctac cgctcggtta
    18781 cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc gctcaaagtt aacactaaag
    18841 cccttctcct gcaactgtag catgcggcga tgcgcacgtt cttccgagga ggcgtcaagg
    18901 aaaattttca ctggtgcatc agggaatacc accgttccca tgtcgcggcc atcggcaatc
    18961 agacctggta attcgcgaaa cgcgcgttgg cgacgcaata atgcttcacg aacgcgtggg
    19021 aatgtcgcga cttgtgaagc cgcattcgcc acttcctgag tacgaatttc gccgctgaca
    19081 tcttcccctt cgaggatcac ttccagattg ccattggttg acacaaaacg tacatccaga
    19141 tgggatgcca gcggtaccag cgcatcttct gacgcaacat caacatggtg atgtaatgcc
    19201 gccaatgcca gtacgcgata aattgcaccc gaatccagca gatgccattg caatgcttcc
    19261 gccatagcct tacacaaggt gcctttccct gcaccgcttg ggccatcaat ggtaataacc
    19321 ggggcaattg ccgtcatctt tatctcctta aaacaggcgt accgttaaca taaccgcgcg
    19381 cattatacgc gccgacgcca caaatcgtta acgttgtgtg caaaaaccag ttttgtctgt
    19441 attcagatgg ggatttattg cgcaattata gcgggctggc agaagaccag cccgaaagat
    19501 gacaatgatt tacttaccag aggcgatacg atcacggatg tgttgcgcac gctctgttga
    19561 tggtgggtga gagtcaaaca tggactgggt gcgaccgcca tccagactag ccagtttttc
    19621 aaagctgcca accagcccct aggtgctgat gccacgtttc ttcaacagat caaaggagaa
    19681 atcatctgct tcggactctt tatcacggga gtatttagca ttaatagcgc cttctgcgat
    19741 atcacccaat tgtgagcggg aaagctggga agccacaccg ctggtagctg aaatggcatc
    19801 gcgtgcggca acgatcgcat aagaaacttt catttcagcc agcgagtgac ccaacgcgac
    19861 gtggcccagt tcatggc
//
LOCUS       DAJVTY010000066        19801 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVTY010000066 DAJVTY010000000
VERSION     DAJVTY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19801
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.066"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..412)
                     /locus_tag="OQQ66_002115"
     CDS             complement(<1..412)
                     /locus_tag="OQQ66_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCS1905043.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDL"
     gene            complement(381..1589)
                     /locus_tag="OQQ66_002116"
     CDS             complement(381..1589)
                     /locus_tag="OQQ66_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCS1905044.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(1589..3169)
                     /locus_tag="OQQ66_002117"
                     /pseudo
     CDS             complement(1589..3169)
                     /locus_tag="OQQ66_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(3201..3847)
                     /locus_tag="OQQ66_002118"
                     /pseudo
     CDS             complement(3201..3847)
                     /locus_tag="OQQ66_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(3870..4022)
                     /locus_tag="OQQ66_002119"
     CDS             complement(3870..4022)
                     /locus_tag="OQQ66_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCS1905045.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFLCQK"
     gene            complement(4282..5535)
                     /gene="amiC"
                     /locus_tag="OQQ66_002120"
     CDS             complement(4282..5535)
                     /gene="amiC"
                     /locus_tag="OQQ66_002120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1905046.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5767..7098
                     /gene="argA"
                     /locus_tag="OQQ66_002121"
     CDS             5767..7098
                     /gene="argA"
                     /locus_tag="OQQ66_002121"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1905047.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7160..8986)
                     /gene="recD"
                     /locus_tag="OQQ66_002122"
     CDS             complement(7160..8986)
                     /gene="recD"
                     /locus_tag="OQQ66_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1905048.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     TLTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(8986..12528)
                     /gene="recB"
                     /locus_tag="OQQ66_002123"
     CDS             complement(8986..12528)
                     /gene="recB"
                     /locus_tag="OQQ66_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1905049.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12521..15409)
                     /gene="ptrA"
                     /locus_tag="OQQ66_002124"
     CDS             complement(12521..15409)
                     /gene="ptrA"
                     /locus_tag="OQQ66_002124"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1905050.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15585..18953)
                     /gene="recC"
                     /locus_tag="OQQ66_002125"
     CDS             complement(15585..18953)
                     /gene="recC"
                     /locus_tag="OQQ66_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1905051.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(18966..19289)
                     /locus_tag="OQQ66_002126"
     CDS             complement(18966..19289)
                     /locus_tag="OQQ66_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1905052.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19274..19681)
                     /locus_tag="OQQ66_002127"
     CDS             complement(19274..19681)
                     /locus_tag="OQQ66_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1905053.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(19678..>19801)
                     /locus_tag="OQQ66_002128"
     CDS             complement(19678..>19801)
                     /locus_tag="OQQ66_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1905054.1"
                     /translation="VVRQDVSGFSPVLTVNMRAASKSEPQTVVNASYSVTGFNL"
BASE COUNT         5001 a         5282 c         4618 g         4900 t
ORIGIN      
        1 ataaatcaca acttatcgca agcgatgagt gatgactgcg gataaaagag atcaaataat
       61 tacccatttt tttcaaacta ggcagtgtcg ccaggatttc cgccgtctca ccactgtttg
      121 aaataagaat aacaacatcg tctttcgcca ccatcccaag gtcgccgtgt accgcttccg
      181 taccgtgaac aaaaaacgag ggagttccgg tgctggcaaa tgttgcagca agttttctgg
      241 caataatgcc agatttccca acaccaataa aaacaacttt tccctggcaa gattgtaata
      301 ttttaattaa attcgcgact tccgaaaggg gggaatcaat aacctcttct actgctttat
      361 gttcgttacg cataaaacgt ttaataagat ggataagatc cgtattgttc attttctttt
      421 tcctgattaa tatttttaat tacactcgca aggcgcgcaa gccccatata taatttttct
      481 cgactgcagg ccagattaag acgcagatag ccctctcctt cagtaccata aacatgcccc
      541 gccataatac ctactctccc ttcgttaatt aatcgacggt aaaatttatc aaagggataa
      601 ccaagggccg agcaatcaac ccacgaaaaa tagcagccct cagctggcca tacgatcaat
      661 tccggaatat tttcatttaa atactgcaag gtaaattgaa tgttagaggc aatatgtacc
      721 tttaatgact ttaaccattc ttcacattca ttataggcaa caatggtcgc aattacattc
      781 agtactgatg gggagtttac gaaatcgcga tacctcgata tcgttctgaa cttatgatta
      841 aaattatcgt cagttgaaat catatatgat ccggttagcg caggaacatt aaatgttttc
      901 gaaatcgaat taaacaccac cactttgtca tcttttattg ttagcgctga cgtgaaacta
      961 tgattaaaaa caaaatcact atgtatttca tctgaacata accaaatatt atgccttgca
     1021 cagcatgctg cgattctgtg aagatcgtct tccgaccaga ccgttcctgt cgggttatga
     1081 ggattacata ataacaccat cttcgcgctt ttaattttaa gctcaaagtc ttgccagttg
     1141 ataacgtagc ctctcccatt ttctttcatc aaagaggttg ttagcatgtt tcgaccatta
     1201 tgcttaatca catcaaaaaa tgcgttataa acaggcgtga aaatgagaat attatctcca
     1261 ttcaatgtca atatttctat tgccttagat attgaataca tcacgcttgg gctataggtg
     1321 atccattctg gttgaatctc tgttttatat tgttgatgat accaattgac tatcgaattc
     1381 agatagtcat catgctgcca acgactgtac cctaaaatcg ggtgttccag acgttttttt
     1441 aatgttctga tgatagcttc cggtatcgga agatccatat ctgaaataga gaatggtaat
     1501 acatctttta caccaaatct gtcttcacaa aagtcccatt gtgtgcaata tgttccttta
     1561 cgagaaattg aggtattaaa aatatccatc aacatatccc catgtaatta tccatatccc
     1621 gacgtagggc gatgactttt gtaccgataa tgacttgtaa ggtattttga tcaagtttca
     1681 caaccgcaat tcctccggcc ctttttattg cagcctcatt tatcaatccc atatccgcga
     1741 tagttaaacg caaacgagtt acacaattat ccagtgattt aatattttct ttaccaccca
     1801 gagccttaac catttgctcc gctgcctgca actctgattt atcatgaact gggatgttag
     1861 ctattgcagt ttcttcgtga gcgttcagcg tgacaggact ggactcttca cggcctacag
     1921 tcatcaggtt aaatttgaga atggtccaac ggaacacaaa gtaataaatg aagaaataag
     1981 ccacttcaac acataccgct aatggccatc ccgttcttgt gccacgtaag acaccaaagc
     2041 taataaattg aatcagatcg ccagtaaagc ccatcttcac gcccatatat ggcagaacca
     2101 tgttagccaa tccattcata aaggcatgga agacaaacaa cagcgggctg acaaataaga
     2161 acagaaattc aatcggctcg ctgacgccgc caataattac cgtgagcaca ccggaaatca
     2221 gcaatgcttt gatagccctt ctgttttgag catgagcagt gcgatacatc gccagcgcgg
     2281 ctccagccag accatactga atcatcagtt tgccctgctc cattttcccc gccaaatcca
     2341 gcggaatgac cgcattctct ttgacgtatg ccataaacat gttcagggtg ccgtaatact
     2401 cttcaccgcc aatcactgcc gatccgccga ttggggtaaa gcggaaaact gatgtcacca
     2461 agtgatttaa gccaaaaggt atcgtcacgc gttcggcaac agcatagaag aaataaccaa
     2521 caggaccgga agttgaaatc cagtgaccaa tgagcatgaa aagattgaaa aatggcggcc
     2581 agataaaagg aatgatcaga ccaaatccac tcatcacaat cagtgtaatg ataggcacaa
     2641 ggcgtgggcc actataaaac cctaattatt caggaatacg taagttaacg atctttttat
     2701 acatgctggc gactaataac cctgccacaa tcccacccaa cacgctggta ttgtaggact
     2761 ggatcccgag tatgatggtt tgcccatgtg tcgacatttg gtcagcagct accaataagt
     2821 cgtgctgttt aagataaaag ttcgttccaa gatgcatcgc cataaagcca attaagccag
     2881 aaaaagcgcc ataggcttta tcatctttct cttttaataa tcccaaaggt atagctatcg
     2941 caaacaatac aggtaaatta acgaaggcaa acaaaccaag gctaacaata aaatcaagta
     3001 tggttttaat taacggaata gccaacactg gaatgaattt tgccatatca tcgctggcta
     3061 aaccacttcc cagccctaac atcataccac atacacttaa cagagcgatg ggatacataa
     3121 atgccttccc caggctctga aaaaaactcc aggatttctt ttgtttcata ctggttatct
     3181 catgaaaatg ttatgtataa ttagtccatt aatactttga tgcgaataga aagatatagt
     3241 ttctcttcca gaatcaaatc atatttaaaa gttgcgtaca gatatctttc aatttcatga
     3301 atggcacgtt gtaactgaaa atctttttca agaaactgct catagatatc accaatattt
     3361 ttctgtttta ttttttcatt acataacaca cgactagaaa aatatttaag gtgcatcata
     3421 aatctggagt agttaaatga acttctgtcc acccttctgt tataaacaac ttcaataagc
     3481 tccgtgattt tttgagacag ctcaataatc cttactgatt cataatcaac tgaattttca
     3541 taattattta aaatatgcaa tactataaaa ccaatctcat catccggtag ttgaacgcta
     3601 aattgctcat taatgattgt taatgcatca cgacttattg catactctgc tttgtacaac
     3661 gctttgattt cctcaaggaa aacattatta atttgtatgc ctgagacgac tcgggatatt
     3721 gcattcgaaa tgtgatcagc aagaatcagt agtagcgatg gattgagttt ttttcgagat
     3781 tactctccgc ataatgcact atcttttcgg caacaaaaat atactcaata cccacatgct
     3841 caatcaattt gtagagttta tttttttgct catttctgac ataaaaatcc ggtttgcagg
     3901 atgctcaggg accgacatac catattttta ttgtaaccaa cgccaggccc agaaatgata
     3961 acctcctgtc cgttcaccga tgcctgtaca caattattgt tcatgacttt ttcgatgatc
     4021 attccatggc tccccaaaaa aaaggcaagg catgtcagaa gtaataatta cttcaaacat
     4081 tgccttgcct gatttaacag ttgcaagata attaactaat gcgtatacct tacacggaat
     4141 tgtgcttaat aaaagagatg acagacttat tttttaagcc gatattcaaa tattcgacag
     4201 cagtcacaaa accatcgttc aacatttatg taaataatat ttattcaatg aaacataaat
     4261 tcattgaata aattaaaaac atcatcccct tctcgccagc gtcgccccat cggcaaaata
     4321 cgctttaatc cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag
     4381 tttacgctct tcctcaacgt tactaataaa cgccgtttcg accagaatgg agggaatatc
     4441 tggtgccttt agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat
     4501 tttaccgagc ttattcagta ccgctttacc aaacttcagg ctgtcggcaa tggtcagcga
     4561 ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc
     4621 aatcaagtcc gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcacc
     4681 tttggttgag agcgcaaaca cagaggaacc gctcggctga cgactggtaa aggcgtcggc
     4741 atggatagag acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa
     4801 cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat
     4861 cagagagcgc agacggcgag ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt
     4921 ccccaccgca ccggagtctt cgccgccgtg gccagggtca agcataataa caataggacg
     4981 atcgcgccct gctttacccg gttgtggacc actttgtgct ggcggcacct gcttttcgag
     5041 gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt
     5101 ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa
     5161 cagctgcggt tttacgtttt gctttaattc aaaaaccatc cgtacggtct gcgggtcaaa
     5221 ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc
     5281 cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact
     5341 caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacac gggtgtagct
     5401 ggacgcaggc cagacgcgca ccgccacgac ctggctgacc gcagccagac tgacctgact
     5461 tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt
     5521 gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg
     5581 actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa
     5641 cattcttttt atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac
     5701 ttgcaccctg cgaaaaaaca gaataaaaat acactaattt agaataatca tgcaaagggg
     5761 tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc
     5821 tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag
     5881 catgagaatt tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt
     5941 ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggccgc gcatcaccac
     6001 gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag
     6061 caggctgcgg gaacattgca actggacatc actgctcgcc tgtcgatgag tctcaataac
     6121 acgccgctac agggcgcgca tatcaacgtc gtcagtggca attttattat tgcccagccg
     6181 ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa
     6241 gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt
     6301 tcagtcactg gcgagagctt taatctgacc tcggaagaga ttgccactca actggccatc
     6361 aaactgaaag ctgagaagat gattggtttt tgctcttcac agggcgtcac taatgacgac
     6421 ggtgatattg tctcagaact tttccctaac gaagcgcaag cgcgggtaga agcccaggaa
     6481 gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc
     6541 cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa
     6601 gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt
     6661 cgtcgcgcaa caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag
     6721 caaggtattc tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc
     6781 attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag
     6841 aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc
     6901 gaggttctgc tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt
     6961 gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt
     7021 gatttactgc ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg
     7081 atggcggatt tagggtaagc gatgaaaatt gtcggatgcg acatgcgtaa cactcgtgca
     7141 tcgcatccgg caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc
     7201 gctcagtacg agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac
     7261 ggcgacgcgc gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt
     7321 ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca
     7381 tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt
     7441 ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc
     7501 cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcga ccttcgtacc
     7561 aacgagagtg cggatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct
     7621 cattcagtcc agccacgcca aacggccctt cccgcagggc gcacaaaagt tggtactcat
     7681 tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc
     7741 cataacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc
     7801 gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac
     7861 ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt
     7921 gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt
     7981 gagtccccgt caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat
     8041 aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc
     8101 cgagaaagat cactcgcgca tgatcgggca aggcgtcgat cagtctcgac atcataggca
     8161 gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat
     8221 gatgacgtaa acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat
     8281 cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga
     8341 gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc
     8401 gttcgccgtc ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac
     8461 cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt cagcgtcact gccgctgcaa
     8521 ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta
     8581 gagcttcatc aacctcaatg gcatgattca cctcattgaa gaagcgcgct accgtccgct
     8641 cgttacacca catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt
     8701 catctcccct gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac
     8761 cgatttcact gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg
     8821 aaagcggcaa acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg
     8881 tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta
     8941 gctgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag
     9001 ggtcataccg gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt
     9061 gtaaatccct tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc
     9121 gccaaagtga cgatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc
     9181 cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc
     9241 ctgttgggtg taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta
     9301 ataacgccct tcgtggcgga atactaggtc gataaagcct tttaacatgc cgcgtacctg
     9361 catgaactcc agcggcgggc agccagcgga tagcggatca aactggcgaa ttagtgcatc
     9421 aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt
     9481 atcgcggtcg gaaagctgac tcaggctaac ccccttttca ttgagaggtg cctggaggac
     9541 agccgtgatc cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag
     9601 tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact
     9661 gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc
     9721 ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc
     9781 gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg
     9841 caatcgttgt agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg
     9901 ctggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat
     9961 acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc
    10021 actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac
    10081 gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc
    10141 cgccagacgt tcgacctccg cgaggtcgac gctttttggc gcagcattaa gatccaaaac
    10201 tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat
    10261 aaatggcaac cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg
    10321 caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc
    10381 gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc
    10441 ttcttgtagc agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt
    10501 tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac
    10561 gccacgtttg cgccagattt gccgataacc atcgaactct tcaactaccg catcccacgc
    10621 atgttcgtca ttattcagcg tttcgatatc cagcgcgttc agccccatca ttgacgttgc
    10681 cagcgcacta cgcagggtgt tctcacgttc gggcgtcatc accgcctgca ataaccaaag
    10741 catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg
    10801 gatttccagc aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag
    10861 cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc
    10921 gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt
    10981 actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg
    11041 ctgcgtttca cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg
    11101 aataaacggt atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac
    11161 actgttcacc attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac
    11221 ttcactacgc gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg
    11281 cttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa
    11341 aattcggtac tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg
    11401 cgtacggatc gccgctgcca acacctcacc gctttcgcta cgcagcgcgg aatcgagccg
    11461 acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc
    11521 gcggatctca gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag
    11581 cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccag ctttcgtgcg
    11641 atcttctaag aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgttggt
    11701 ctcttcttct gcccaggcgc tgatcttgtc gatccattta gcctgattgc tacggttaaa
    11761 cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg
    11821 ccactgctgt tttaccgtag caatacgcgc cacaatttgc gcgtggcggg aagccagcgt
    11881 ttcatcatcg ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat
    11941 atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg
    12001 cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtaac gtagcagaga
    12061 ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag
    12121 catgcgctgg caaaaaccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc
    12181 taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca gacgttcgta
    12241 cagtggattg tcggtagttt cacgcagaca ggcgatgcgc aactcatgga tattgctacg
    12301 gatacgacca cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc
    12361 aacggtcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata
    12421 gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcacc
    12481 ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca
    12541 tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcagggt
    12601 gtacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt
    12661 gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta
    12721 tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac
    12781 ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg
    12841 cctgctggat ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgtggttg
    12901 ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa
    12961 ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca
    13021 attgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag
    13081 agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg
    13141 cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta
    13201 catctttgtt tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg
    13261 cctgcgttgt tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc
    13321 ctgattttaa ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt
    13381 tacgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg
    13441 cctgctcaaa cgctttaccc ttttctgcgg aatccatcat ctggttatac caagacttcg
    13501 cctgctcaag ctgatcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga
    13561 acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt
    13621 tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc
    13681 ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg
    13741 gattacgcaa aatcaggctg acatcagctt tgggttcgct ggaaaaataa cggcttggcg
    13801 catacaccac gcgcagattc gactcatcaa caatcagctc tggatggtca tatttcttct
    13861 ctgacttaat cagcgagaaa tcgtcaggaa tataggggtt aagctccggt aaggagagcg
    13921 caatattggc agctttttgc tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct
    13981 gatacggcgc atcgacaaaa taagccgttt tattgtgcgg ctctttcggg ctgatatacc
    14041 agatacgcgc attctgcggc gtcatcatcg ccagacgttc ttttactgct ttagcatcgt
    14101 accgatcggc aatattgact gcatccagcg tatgctcaac cggaacgcga atcatggtat
    14161 ctgccagcca ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat
    14221 ccagcacatt cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat
    14281 tgagatagct aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcggtta
    14341 aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc
    14401 taatgccctc aactaatccc tgcttttgca gccagtcgga aagtgtacct gggctgcgat
    14461 taccaatcag ataggtaatc aattcatccg ttttactacg gaacttcgct gagttgttat
    14521 cgatgcgaaa ctcaacgcgc agaactttac gcggcagtgc cgggacgtaa tgaatgataa
    14581 tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt
    14641 tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattgc
    14701 tgtaaataac cgccttcatc agattggcgg aatagtactt ctcgtggaaa tctttcagcg
    14761 cctgctgcac cggattacca ggtttatcgc ttaaagtttc gaggttacca ccagaaaact
    14821 ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt
    14881 cacgcgtacg cgccatggtt aattcagcgt tcaccgcatt acgctcacgt tcggcatatt
    14941 tcttgtcgag caaaggttca gcaatagcat cggccaggcg gtctaccgca ccaggcaagg
    15001 cgtcgttctc aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac
    15061 taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc
    15121 ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca
    15181 gcgacccaac gggcaccacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca
    15241 gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac
    15301 ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt aagggtgccc
    15361 aaagggcaac taacaacaat aatgctttga accaagtgct gcggggcatt cacggacctc
    15421 ataatcaact taattttctg tccagattca acacgttaac gcaaatcatc tgccagaatt
    15481 taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg
    15541 catcataaag taagcggata gattgcgcaa tttttataca gcactcatga ctgattaaag
    15601 cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt
    15661 gttaattgcc gccagagcct ttgataccag atattatcac cttcaccacg caccatcatg
    15721 ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc
    15781 agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt
    15841 aacaccagca atggcgcgga catcccttca cgatacccct caatcagttg tgagaggtaa
    15901 tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa
    15961 aggcgacttt caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc
    16021 attccctgcg ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc
    16081 tgcggcaacc agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc
    16141 cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgagtt
    16201 tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcgacgg
    16261 aacaagcgtt cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga
    16321 taacggctaa gcccttccag aataaatggc tcggtgtcgg ggatttcgct gtcttcggta
    16381 cggaagttca cctgcaaacg catctggaaa aatgcccgca ccggatgtgc ccagaatcgt
    16441 tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca
    16501 gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt
    16561 tcgcctggct ggtagttttg tggatcaaac ggcatccggg tatggaggca ggtaagatgc
    16621 gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga
    16681 ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta
    16741 tcctgaatgg aaagcccgat ataactgata tagagttttt gctgcgcgga aattaacgct
    16801 tccaggaaca ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg
    16861 ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc
    16921 agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc
    16981 ggtccggcga gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca
    17041 cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc
    17101 gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag
    17161 aaggcgttga gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc
    17221 agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct
    17281 gcaattaagc cgctcgactc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc
    17341 tccatcgcgt agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg
    17401 gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg
    17461 ttgacccaca ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc
    17521 accggcacat ccagtaatgc cagcacgtct tccgacacaa agcggctgtc tggcaacgat
    17581 aacaggctga taaacgcttc cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg
    17641 gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg
    17701 tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct
    17761 tccagcatcg ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa
    17821 acgtggaagg tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa
    17881 aactcttcga tgttcacacc agcaacggca cggttttcca gttccagaat gtcagactga
    17941 atgttatgca gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc
    18001 tccaggtcag aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc
    18061 gggttgccga catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg
    18121 cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc
    18181 gccagataag caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg
    18241 agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac
    18301 gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac
    18361 tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt
    18421 tcatgggtat attccaccag cgccttccac aacggcgctt gccaggcctg tgcttctccc
    18481 agcccttcaa ccaaatgtcc tgtttcccat tgtgccagcc actccgggcg atagaccaga
    18541 tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta
    18601 tcgtcagtca gataatgccg caacagggta aagtcttcgt gctccaacag ttgcggcagc
    18661 agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatctcc
    18721 ggtaacaccc ggacgaacat atcccagata aagctcgcag gcagcggaaa atcaatgttt
    18781 gccgcaatac caaacttttg cgacagggtc atttgcagcc actgtgccat accggtactt
    18841 tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac
    18901 tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct
    18961 cctgactact gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt
    19021 gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcaa tcggttgacc
    19081 tgccagttag ctggtggcga aatcgcgcgc agttgcgttt gctgccagcc atgccgccag
    19141 agctgttggt actggtttct gctggcaaga ctgttcatta atgttcgctg gaaacccgat
    19201 aacgcagtga caatcatcac catcaacacc atcgccaaca ttacctccgg caggctaaag
    19261 ccttgttgat tcttcaggga agctgacata acgccctctc tttcagcgga caaaaatcgc
    19321 tccagccgct tggcgaaaaa acaatgtttc catcaatgac ttcgcctgtt cgccacaacg
    19381 aaacgccttc ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa
    19441 cctgggcatc ggtttcagcg tactgcgagc actgaactgc tggctgcgtc tgccaggagt
    19501 gcatttttcc ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt
    19561 ccatgctcac gcgagaggca aaactgcgat cctgttggct cattccttgt aatagcaaac
    19621 tacccaaaac cagcagcatc aggaccagag ccagtgacga aacacccttt tcgcggttca
    19681 caggttgaat cctgtcacgc tatagctggc attcaccacg gtttgcggtt cagacttact
    19741 ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac
    19801 c
//
LOCUS       DAJVTY010000067        19798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVTY010000067 DAJVTY010000000
VERSION     DAJVTY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19798
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.067"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1988
                     /locus_tag="OQQ66_002129"
     CDS             <1..1988
                     /locus_tag="OQQ66_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCS1905055.1"
                     /translation="GVGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRD
                     TFNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDV
                     NQVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQH
                     NTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHD
                     INDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKIL
                     TNQTNLTSTFYTSSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLT
                     RNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITC
                     PAGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQ
                     SGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQ
                     YGKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTL
                     WSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQL
                     NVYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2030..2707
                     /gene="ybiX"
                     /locus_tag="OQQ66_002130"
     CDS             2030..2707
                     /gene="ybiX"
                     /locus_tag="OQQ66_002130"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1905056.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2781..3047
                     /gene="ybiI"
                     /locus_tag="OQQ66_002131"
     CDS             2781..3047
                     /gene="ybiI"
                     /locus_tag="OQQ66_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1905057.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3312..3572
                     /locus_tag="OQQ66_002132"
     CDS             3312..3572
                     /locus_tag="OQQ66_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1905058.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3800..4885)
                     /gene="hcxB"
                     /locus_tag="OQQ66_002133"
     CDS             complement(3800..4885)
                     /gene="hcxB"
                     /locus_tag="OQQ66_002133"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1905059.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5026..5988)
                     /gene="ybiB"
                     /locus_tag="OQQ66_002134"
     CDS             complement(5026..5988)
                     /gene="ybiB"
                     /locus_tag="OQQ66_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1905060.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6016..8166)
                     /gene="dinG"
                     /locus_tag="OQQ66_002135"
     CDS             complement(6016..8166)
                     /gene="dinG"
                     /locus_tag="OQQ66_002135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1905061.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGRHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8286..8768
                     /gene="ybiA"
                     /locus_tag="OQQ66_002136"
     CDS             8286..8768
                     /gene="ybiA"
                     /locus_tag="OQQ66_002136"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1905062.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(9000..10364)
                     /gene="rhlE"
                     /locus_tag="OQQ66_002137"
     CDS             complement(9000..10364)
                     /gene="rhlE"
                     /locus_tag="OQQ66_002137"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS1905063.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10593..11264
                     /gene="cecR"
                     /locus_tag="OQQ66_002138"
     CDS             10593..11264
                     /gene="cecR"
                     /locus_tag="OQQ66_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1905064.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11267..12262
                     /gene="hlyD"
                     /locus_tag="OQQ66_002139"
     CDS             11267..12262
                     /gene="hlyD"
                     /locus_tag="OQQ66_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1905065.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12255..13991
                     /locus_tag="OQQ66_002140"
     CDS             12255..13991
                     /locus_tag="OQQ66_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1905066.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13984..15117
                     /locus_tag="OQQ66_002141"
     CDS             13984..15117
                     /locus_tag="OQQ66_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1905067.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15128..16234
                     /locus_tag="OQQ66_002142"
     CDS             15128..16234
                     /locus_tag="OQQ66_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1905068.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFTRASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16196..16606)
                     /locus_tag="OQQ66_002143"
     CDS             complement(16196..16606)
                     /locus_tag="OQQ66_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1905069.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16739..17490
                     /locus_tag="OQQ66_002144"
                     /pseudo
     CDS             16739..17490
                     /locus_tag="OQQ66_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17487..18728
                     /gene="clsB"
                     /locus_tag="OQQ66_002145"
     CDS             17487..18728
                     /gene="clsB"
                     /locus_tag="OQQ66_002145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1905070.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18728..19684
                     /locus_tag="OQQ66_002146"
                     /pseudo
     CDS             18728..19684
                     /locus_tag="OQQ66_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19720..>19798)
                     /locus_tag="OQQ66_002147"
     CDS             complement(19720..>19798)
                     /locus_tag="OQQ66_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCS1905071.1"
                     /translation="FIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT         4619 a         5365 c         5244 g         4570 t
ORIGIN      
        1 ccggcgtggg tgcgtttttg gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgtgg tgccgatacc tctaacagta tttatattga tggcattcgc gatatcggca
      121 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccgtccggca
      181 ccgactacgg gcacagcgca ccgacgggct cgatcaatat gatcagcaaa cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacca ccgcggtgcg cctgaatgta atgggcgaaa
      361 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
      481 acaacacgcc agacggcggc attccgacca tcggtttgcc gggctattct gccccatctg
      541 cgggaacggc ggccctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
      601 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
      661 acgataacac caccattcgc aatactaccc gttggtcgcg cgtaaagcag gattacctga
      721 tgacggcgat tatgggcggg gcgtcgaata ttactcagcc caccagcgat gtgaatagct
      781 ggacctggtc acgcacggcg aataccaaag atgtgagtaa taaaattctc accaaccaga
      841 ccaacctgac ctcgacgttc tataccagtt ctatcggtca tgatgtcagt accggcgtgg
      901 aatttacccg tgaaacgcag actaactacg gcgttaatcc ggtgacgtta cccgcggtaa
      961 atatttatca tcctgacagc agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg
     1021 ccaatggtca gacggatacc ttcgcaattt acgcctttga tacgctgcaa atcacccgtg
     1081 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcca
     1141 ccgcctgcgg cggcagcgga cgcggtgcca tcacctgccc agctggtgtg gcaaaaggtt
     1201 ctccggtcac caccgtcgac accgccaagt cgggcaatct ggtgaactgg aaagccgggg
     1261 cgctgtatca cctgacggaa aacggtaatg tctatattaa ctatgccgtt tcccagcagc
     1321 ctccgggcgg caacaacttc gcccttgcgc agtctggcag cggtaacagt gccaaccgca
     1381 cagattttaa accgcaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
     1441 ataaacgcct gttgctcacc gccgcactgt tccgtactga tatcgaaaat gaagttgagc
     1501 aaaatgatga cggaacttac tcgcaatacg gtaagaaacg tgtcgaaggc tatgagatat
     1561 ccgtggccgg gaatatcact cccgcgtggc aggtgattgg cggctatacc cagcaaaaag
     1621 caaccatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccgtataccc
     1681 cggagcacgc cttcacctta tggagccagt atcaggcaac cgatgatatc tctgttggcg
     1741 cgggcgcacg ctatatcggc agtatgcata aaggttcaga cggcgcggtg ggaacgccag
     1801 cgtttaccga aggttactgg gtcgccgatg ccaaactggg gtatcgggtt aatcgcaatc
     1861 tcgacttcca gctaaacgtt tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca
     1921 agagcggcta ccgttatcac ccgggcaagc caagaacctt cttgctcaca gccaatatgc
     1981 atttctgatt cagatgtggg gcgcaggccc cactttttgg agaaattgta tgatgtacca
     2041 cattcccggc gtgttatcgc cacaggacgt cgctcgtttt cgcgaacaac tggaacaagc
     2101 cgaatgggtg gatggacgcg tcaccaccgg cgcacaaggt gcgcaagtta agaacaatca
     2161 acaggtagac acccgcagca cgttatacgc cgccctgcaa aatgaggtgc tgaacgcggt
     2221 taaccaacat gctttattct ttgccgcggc cttgccgcgt accctttcca cgccgctgtt
     2281 taatcgctat cagaacaatg aaacctatgg ttttcatgtg gatggcgcag tacgcagcca
     2341 tccacaaaac ggctggatgc gcactgacct ttctgccacg ctgtttttaa gcgatccaca
     2401 aagctacgac ggcggcgaac tggtcgttaa tgacaccttc ggacaacatc gggtaaaact
     2461 cccggcaggt gatctcgtgt tgtatccctc cagcagcctg cattgcgtga cacccgtaac
     2521 ccgcggcgta cgagtggcat catttatgtg gatccagtcg atgatccgcg atgataaaaa
     2581 gcgcgccatg ctgtttgaac tagacaaaaa tattcagtcg ctgaaaagcc gctatggtga
     2641 aaatgaagag atcctgtcgc tgctcaatct ttatcataat ctgctgcggg aatggtcgga
     2701 gatctgacgc ttgaactgcc caggttcgtc cctatattag ctacacttaa ctgtacaagc
     2761 attgatatgg ggaggacgat atggcatccg gttgggctaa cgatgacgcc gtcaacgaac
     2821 agatcaacag tacaattgaa gatgcgattg cccgcgctcg gggtgaaatt ccgcgtggcg
     2881 aaagcctgta tgaatgtgaa gagtgcggtg cccccatccc gcaagctcgt cgggaagcca
     2941 ttcctggcgt gcgcttatgt attcattgtc agcaggagaa agatttacaa aaaccagcgt
     3001 atacaggata taatcgcaga ggttcgaaag acagccagtt acgttaacta caactggcga
     3061 gatgcatagc gagtataatt tgtattttgc gtggcattcc gtgcctttaa cgctgctgtg
     3121 actgccacgc ggcataaaac aagtccataa gtacaacaaa taaatggttt atcagtatgt
     3181 tagatgtcaa tcaatttatt tgaacaaggc ggtcaattct cttcgatttt atctctcgaa
     3241 aaaaaacgtg atactcatca catcgacgaa acaacgtcac ttatacaaaa atcacctgcg
     3301 agagattaat tatgaaaact atcaatactg ttgttgctgc tatggctctt tcaactctat
     3361 catttggcgt gttcgcggcg gaaccggtaa cggcatccca ggcacagaac atgaataaaa
     3421 tcggcgtggt ttctgccgat ggcgcatcca ccctcgatgc cctggaagcg aaactgactg
     3481 agaaagccgc ggcagccggt gccagtggat acagcatcac ttctgccacc aacaacaata
     3541 aattaagcgg tactgcggta atttataagt aattcgtagg acggataagg cgtttacgcc
     3601 gcatccggca acagatgcct gatgcgacgc taccgcgtct tatcaggcct acaaaatccg
     3661 aaccgttgga cggatcaggc ggttacgcta cgtccagcac aagtatttcc ctgtctgttt
     3721 gccgacagac gcatatgctc taaccctcat tgatcctata ttacccttgt ttgcccgtcc
     3781 gttactggac gggctttttt tagctggcta actgctgaca gaaagcctgc aacgtctctt
     3841 ccgacatacc aatctgccgc gccgcatcac aaatggcctg ccagcttccc gcatccaatg
     3901 gaatcccctg ctcctgccgt tcgcgacgcg tgttcacttc ccactcgccc ggtagcaaaa
     3961 tcggcttatc gtcatcatgc ggcgaggctt tcacccactc ggcaaaggct tcagtctgcg
     4021 cgctacaatc cggcgcgccg aacagttccg ggttgatgat gatagtggtc atgcagttaa
     4081 gaatggcatc gggactggtt tgtaacgttt cctgatgcgt cgttttaccg ccagaaagcg
     4141 ccccgccaag aatttcacac atcgctgcaa gggcgtagcc tttatgttcg gcaaaggtca
     4201 acagcgaccc caacggcgac tcctgcatta ccgccggatt ggtcgtcggc acgccgttaa
     4261 cgtcaatcag gcaacctggc ggcacgggga cgcctttatg ccaggcgacg cgagttttac
     4321 caaatgcgat ggcgctggtg gcgtaatcaa gcaacagcgg gaaattatct ttacgaggga
     4381 aaaccacaca gaacggattg gtgccaaagc ggctgtcgcg accgtggaac ggtgcgacca
     4441 ttggaatacc gaccacgcta acaaagtgga tagagacaaa ccccgccgct gcacactgct
     4501 ccgcccagta accgatacgg ccgatatgat gcgagttatg tagcgccacg gcagcaatac
     4561 cgtgctgatg cgctttctca atccccagcg ccatcgcttc gtgtgccgcg acctgaccga
     4621 atgcgcgatc gccatcgagc gtgactgccg cccccgcctc tttaacgatt ttggcatgat
     4681 ggttaatttg cagatgcccc tgactccagg aacgcacata gcttgggatc atgccaatac
     4741 catgagaatc atgccctgcc aggtttgccg cgattaaatg atcggccact aatttcgctt
     4801 cttgttcctc gctacccatc tgacgaaata cagcctgaat aaaactgtgc agcgtctgag
     4861 catcaaagcg atgaccactt tccatggtta actcctgttt atgttatgtg tttgttgttt
     4921 ttttatgctg cacgccgggc gcgggaaggt caataaagat gcaggaattt catattgcgg
     4981 tggggtaaaa gccggatgac atggctcatc cggctgagaa aagaattaaa atgcctgatt
     5041 aacgcgcgcc aggccgtcgc tgatagttgc cgcttcaccc gtagcctcca ggcagcaagc
     5101 catctggatt ttcagcgatt cgggaatcgg ttcgctgcca gcaaggcaac gctcaatcca
     5161 ctgcgccgtg gtttctgggt cttttgcttg tggcagtaac tcgctaccag cagtgtcctg
     5221 tttttcatac agcacccgca tcccttcacg gtcaatgaga ttgatctgcg ggcagcgctg
     5281 cggattagca tacacttcgc cttcagtacc atgcattagt agcgcccgcc cgccgatatc
     5341 gctaaagaac ttcgcgactc gcccgatgta ttccggatgc gaaacgctgg aaagacgcag
     5401 cgcctcacct tcggcaaatg gcgtcgccag tttcgccagg gtatgtgcac tgttacgcac
     5461 gcccatccgc cagcgcatcg ccagttgttt ttccagcggc gggcaaaacg cgccgacagg
     5521 cataaacacc ggttgatgtt cgtcgagctt cgcctgcgcc tgcccgccgt gcaacgttgg
     5581 tgtaataccc atcagttcga aaatggtttc tgtcagcacg cgagttggat cttcgctaac
     5641 cccgtgaacc accacaggaa aaccgagttt gtgcaggaga atagccaaca acggcgtcag
     5701 gttggcctgt ttacgcgcgc cgttgtaact ggggatgaca atcggcatcg gcttgcctgc
     5761 aggcggcgtc agcttgatgg tgtgattttg catggcttcg taaaagccga gcatctctgc
     5821 ttccccttcc cctttgatac gtagcgcaat caatacgccg cccaactcaa ggtcagggac
     5881 ttcaccattg agcatatggg catacagacc gcgcgcagta tcccggtcta aatcgcgcgc
     5941 gtggtttttc ccgcgcccga tctctttaat gattttgcga tagtccattt acgactcctt
     6001 acctgactca catcattaac gccgccgacg gcgtggggat ttcgtttttt ctttcttttt
     6061 aactatacct tcagggactt ccggttgctc tatcggaaat accggtaatg catccagtag
     6121 tcgcttgcca taatttttgg tcagcaagcg tttgtcgtag ataaccactt cgccccagca
     6181 accgtggctt cgaatcagtc gcccaacctg ctgaatcagg ttaaacgagg cgctcggcag
     6241 gctttgcacc tcaaacggat agcggttgag gcttttcagc cattcccctt cggtgatcac
     6301 caccgggctg tcgatgggcg gaaaagcgat tttgtggata tgcacctggc tgagcagatc
     6361 acctttcaaa tcaagccctt cggcaaatga ctgtaagccc accagcacac tacgctcacc
     6421 gttggcaacg cgtttgcggt gcagttcaac taagcgataa cgcggctgat cgccctgcac
     6481 cagcagcatc aggcgtaaat ccgtcacata gtcgagaaag cgctgcatcg cccgtccgct
     6541 ggcaaacagt accaacatac cgagatgttt tttgctctcc acctgctcac ggaaaaaggc
     6601 cgccatttcg gcaatatgct gctcttcgtt gtcgatggaa ggctcaaagc gcatccgggg
     6661 aataacaatt ttgccctgtt cgcagtggtt aaagggggaa tccagcgcca caaaacggtc
     6721 gcccgctttc tctttcagcc cgctcatctc ctgcaaacgc gaaaaactgt tcagcgaacg
     6781 caaggttgcg gaggtgacaa taatgtgcgg aatactgcgc cacagcagcc tttccagctg
     6841 atcgctgaca cgtattccca cgcagtgaaa ccagagatgc agctgccctt cgcgctcttc
     6901 ccgcgtcgcc catttggtca ccggcgcacc ggaagattgc gccagagacg ccagccgcca
     6961 gagtttgctt tgcgcctcga acatccccaa cgcgcggttc atctgcaaaa tcaaccgatg
     7021 cagacgcaca atatcgtggc ggccggtttt ctcacttaaa tcgttaagaa ataactccgc
     7081 cagaccacgc agcatctcgg tgagttttgc cagccgctgg cagatctcca gcacttcatc
     7141 aggcagttcg cccatcgcaa aacggtgctc tgcctcctgc ccggcaggca tgtagagatt
     7201 gagaatgttg tttaacgagg cgataagctc atacaactct tcacaatgcg cattcaaacg
     7261 ttcagggatc gccagcggtg ggatggtctt cgggcgaaac tgctccatgc aggtagcgac
     7321 cagtttcgtg aacaagtcca gctgtagtcg ataccagggc gcggtgattt cagcgctcat
     7381 ctccagcgca tcccgggcca catctggcag gtgatgacct tcgtccagca ccagcagcag
     7441 atttttcggg tcaggcagta cggcttcgct ttccatcgcc gccatcacca gcgcatggtt
     7501 tgccaccacc acttccgctt cctgaatctc ccgacgagca acaaaaaacg ggcattcacg
     7561 atagtagtaa cagttacggt taaggcagct ggctttgtcg gtactcaaac gacgccagag
     7621 atcatcatct atagcgatat ccgtatgatc gcgcagacca tcccatttat aagtgtcgag
     7681 atcgcccttc agcttcgcac aacgtttttg ctcttcctga ttatttggcg tcagttcgtc
     7741 gtcaagaaac gccagcagat cctgttgcgt gggttcagtg ctggcgagcg ccgtcagatt
     7801 acgcggacaa acgtagcgcc cacgcccgaa agcggcagtg aatttaagat cgggaatgat
     7861 ctttttcagc agcggtaaat ctttgctgta aatctgatcc tgcaacgcca cgttggcggt
     7921 actcaccacc agcgtttttt gctcttcgcg ggcaatggcg atgccgggga tcaaatagga
     7981 gagcgttttc ccaacgccgg tgggggcttc aatcgccaga tgccgccctt cttctccggc
     8041 cagcgttttg gcgacgtccg caatcatctg ccgctgcggc gcacggggaa taaagtcggg
     8101 gatctgttcc tgaagcgcct tataccaggc ggcaatttgc gctttaagcg cggcggttaa
     8161 tgccatgaga aaacctgaaa tactgtataa acagccaata ttgtggcatt tttcggtttt
     8221 caggggtaat ggtttttctg tttctggaaa gcggctcgca ataaccacca acataatgcg
     8281 gtttaatgcc cgttcgagca caaaggatcc aacacgtcat gcaagacacc atcataaatt
     8341 tctacagcac cagcgatgac tacggtgatt tttctaactt tgccgcctgg cccatcaagg
     8401 ttgacgggaa aacctggccc acctccgaac actattttca ggcacaaaaa ttccttgatg
     8461 aaaaataccg cgaagagatc cgtcgggttt cttcccccat ggtcgccgct cgcatggggc
     8521 gtaatcgttc caagccactt cgtaaaaact gggagtcggt taaagaacag gtgatgcgta
     8581 aagcacttcg tgctaaattc gaacagcatg cagaactgcg tgcgctcttg ctggcaaccg
     8641 cgcccgcaaa actggttgag catacggaaa acgatgctta ctggggagac ggtggtaatg
     8701 gtaagggcaa gaatcgactg ggctaccttt taatggagtt gcgcgaacaa ttggctatag
     8761 agaagtaacg tctcggtggc ttatgggcgg taaaacagat aaattttttc ccgtgttctc
     8821 aaattgctga caagtgcttg ttgtgaaatg ccggatgcgg cgtaaacgcc ttatccggcc
     8881 tacaaaagca tgcaaattca atatattgca gagttaacgt aggcctgata agcgtagcgc
     8941 atcaggcagt tttgcgtttg ttcatcagcc tgatgccggg catagcccgg cataaaagat
     9001 tactgcgcag cggcaggttt acgcggacgg cggcgacgtt gttgttcgcc tgccggtttg
     9061 gcatcgccga ggcggcgaga cggtttttct gcaggtttcg cgcttgcaga ttttgcgcca
     9121 ccttccccac ggcgtggttg ctgttgaccg cgaccaccac cttgcccacg accgccgccg
     9181 ccacgttgct ggcgaccgtt ctggatcggt tcggctttga ttgacgggtc cggctcatag
     9241 cccggaatcg caatgcgcgg gatctctttt ttcagcagtt tttcgatatc acgcagcagt
     9301 ttgtgttcat caacacacac cagcgacaac gcttcaccgg tagcagccgc acgaccggta
     9361 cgcccgatac ggtggacata atcttccggt acgtttggca gttcatagtt gacgacgtgc
     9421 ggcaactctt cgatatccag accgcgcgca gcgatatcgg tcgccaccag tacgcgaatg
     9481 tcactcgatt taaaatcagc cagcgcacgg gtacgcgcac cttgcgattt attcccgtgg
     9541 atcgccgcac tacggatgcc atctttattg agctgctcag ccagatggtt agcgccgtgt
     9601 ttggtacggg taaacaccag cacctgctgc cagttccctt taccaatcat gtgcgacagc
     9661 aattcgcgtt tgcgtttctt atcgacaaag tgaacgtgct gggtcacctg atcagacgcg
     9721 gtattgcggc gtgccacttc gatttccagc gggttgtgca acagtttttc cgccagggct
     9781 ttaatatcgt cagagaaggt cgcagagaac agcaggttct ggcgtttcgc tggcagtttt
     9841 gttaacacgc gacggatatc gtggataaag cccatgtcga gcatgcggtc agcttcatcg
     9901 aggacgagga tttcaacctg atccagcttc actgcatttt gatgttccag gtccagcaaa
     9961 cgtcccggtg ttgccaccag aacatcaacg ccgccgcgca gcttcatcat ttgcgggtta
    10021 atgctgacac cgccaaacac caccagcgaa cgaatgttca aatatttgct gtaatcacgg
    10081 acgttttcgc caatctgcgc agccagttca cgggtcgggg taagaatgag cgcacgtacc
    10141 ggacgacgcc ctttggcgtg cggctggcga gtgatcaggt gttgcaacag cggcagcgta
    10201 aagcccgctg ttttaccggt accggtctgg gcgctagcca tcaggtcgcg gccttccagc
    10261 accgcaggga tcgcctgctg ctgaataggg gtgggttcac ggtaaccctg ctcggcaacg
    10321 gcgcgcagga tatcagggct taaacccaaa gaatcgaaag acataactac tccgaaccgc
    10381 cccgaccgtt accggtgtag ttttcaggga gatactgaaa aggaaaatga caaaaaccac
    10441 aatgtcatga gggagcggag tgtagcagtt tttgtgacgc agcgcataaa ttatccctgt
    10501 gactggcgtt gctaaaattc ttagccatac tggacaactc ccctatggag tcataatctt
    10561 aatcaatcat ttgattaaga atggactgtg cgatgaataa tcctgccatg acaatcaagg
    10621 gtgaacaggc gaaaaaacag ctgattgctg ccgcactggc gcagtttggt gaatatggaa
    10681 tgaacaccac cactcgcgag atagccgccc aggccgggca gaatatcgct gccatcacct
    10741 actacttcgg ttcgaaagaa gatttgtacc tcgcctgcgc ccagtggatt gccgatttta
    10801 ttggcgagca gttccgtccg catgccgagg aagccgaacg cttgttcgcg caaccacagc
    10861 ctgatcgggc tgccatccgt gaactgatcc ttcgcgcctg caggaacatg attaagctgc
    10921 tcacccagga tgacaccgtc aacctcagca agtttatctc ccgtgagcag ctctctccca
    10981 cggcagccta ccacctggtg cacgaacagg tgattagtcc gctacacagc cacctgacac
    11041 gcctgattgc cgcctggacc ggctgcgacg ccaatgacac ccgcatgatc cttcataccc
    11101 atgcgttgat tggtgagatt ctggcgttcc gtcttggcaa agaaacaatt ctgttacgta
    11161 ccggctggac cgcgttcgat gaagaaaaga ccgaactgat caaccagacg gtgacctgtc
    11221 atatcgacct gattttgcaa ggattatcgc aaaggagttt gtagtgatga aaaaacctgt
    11281 cgtgatcgga ctggcggtag tggtacttgc cgccgtggtt gccggaggct actggtggta
    11341 tcaaagccgc caggataacg gcctgaagct gtatggcaac gtggatattc gtacggtgaa
    11401 tcttagtttc cgtgttgggg ggcgcgttga atcgctggcg gtggacgaag gtgatgcgat
    11461 caaagcgggc caggtgctgg gcgaactgga tcacaagccg tatgagattg ccctgatgca
    11521 ggtgaaagcg ggcgtctcgg tggcacaggc gcagtatgac ctgatgcttg ccgggtatcg
    11581 cgatgaagaa atcgctcagg ccgccgcagc ggtgaaacag gcgcaagccg cctatgacta
    11641 tgcgcagaac ttctataacc gccagcaagg actgtggaaa agccgcacca tttcggcaaa
    11701 tgacctggaa aatgcccgct cctcgcgcga ccaggcacag gcaacgctga aatcagcaca
    11761 ggataaattg cgtcagtacc gttccggtaa ccgtgaacag gacatcgctc aggcgaaagc
    11821 cagcctcgaa caggcgcagg cgcaactggc gcaggcggag ctgaatttac aggactcaac
    11881 gttgatagca ccgtctgatg gcacactgtt aacgcgcgcg gtggagccag gcacggttct
    11941 caatgaaggt ggcacggtgt ttaccgtttc actaacgcgt ccggtgtggg tgcgcgctta
    12001 tgttgatgaa cggaatcttg accaggccca gccggggcgc aaagtgctgc tttataccga
    12061 tggtcgcccg gacaagccgt atcacgggca gattggtttc gtttcgccga ctgctgaatt
    12121 taccccgaaa accgtcgaaa caccggatct gcgtaccgac ctcgtctatc gcctgcgtat
    12181 tgtggtgacc gacgccgatg atgcgttacg ccagggaatg ccggtgacgg tgcaattcgg
    12241 taacgaggca ggacatgaat gatgccgtta tcacgctgaa cggcctggaa aaacgctttc
    12301 cgggcatggg caagcccgcc gtcgcgccgc tcgattgtac cattcacgcc ggttatgtga
    12361 cggggctggt ggggccggat ggtgcaggta aaaccacgct aatgcggatg ctggcgggat
    12421 tactgaaacc cgacagcggc aatgccacga tgattggctt tgatccaatc aaaaacgacg
    12481 gcgcgctgca cgccgtgctc ggctatatgc cgcagaaatt tggtctgtat gaagatctca
    12541 cggtgatgga gaacctcaat ctgtacgcgg atttgcgcag cgtcaccggc gaggcacgta
    12601 agcaaacttt tgctcgcctg ctggagttta cgtctcttgg gccgtttacc ggacgcctgg
    12661 cgggcaagct ctccggtggg atgaaacaaa aactcggtct ggcctgtacc ctggtgggcg
    12721 aaccgaaagt gttgctgctc gatgaacccg gcgtcggcgt tgaccctatc tcacggcgcg
    12781 aactgtggca gatggtgcat gagctggcaa gcgaagggat gttaatcctc tggagtacct
    12841 cgtatctcga cgaagccgag cagtgccgtg acgtgttact gatgaatgaa ggcgagttgc
    12901 tgtatcaggg ggaaccaaaa gccctgacac aaaccatggc cggacgcagc tttctgatga
    12961 ccagtccaca cgagggcaac cgcaaactgt tgcaacgcgc cttgaaactg ccacaggtca
    13021 gcgacggcat gattcagggg aaatcggtac gtctgatcct caaaaaagag gccacaccag
    13081 acgatattcg ccatgccgac gggatgccgg aaatcaacat caacgaaact acgccgcgtt
    13141 ttgaagatgc atttattgat ttgctgggtg gtgccggaac ctcagaatcg ccgctgggtg
    13201 caatattgca tacggtagaa ggcacacccg gcgagacggt gatcgaagcg aaagaactga
    13261 ccaagaaatt tggtgatttt gccgccaccg atcacgtcaa ctttgccgtt aaacgtgggg
    13321 agatttttgg tttgctgggg ccaaacggcg cgggtaaatc gaccaccttt aagatgatgt
    13381 gcggtttgct ggtgccgact tccggccagg cgctggtgct ggggatggat ctgaaagaga
    13441 gttccggtaa agcgcgccag catctcggct atatggcgca aaaattttcg ctctacggca
    13501 acctgacggt cgaacagaat ttacgctttt tctctggtgt gtatggctta cgcggtcggg
    13561 cgcagaacga aaaaatctcc cgtatgagcg aggcatttgg cctgaaaagt atcgcctccc
    13621 acgcgaccga tgaactgcca ttaggtttta aacagcggct ggcgctggcc tgttcgctga
    13681 tgcatgaacc ggacattctg tttctcgacg aaccaacgtc cggcgttgat cccctcaccc
    13741 gccgcgaatt ttggctgcat atcaacagca tggtagagaa aggcgtcacg gtgatggtca
    13801 ccactcactt tatggatgaa gcagaatatt gcgaccgcat cggcctggtg taccgtggga
    13861 aattaattgc cagcggcacg ccggatgatt tgaaagcgca gtcggccaac gacgagcaac
    13921 ccgatcccac catggagcaa gcctttattc agttgatcca cgactgggat aaggagcata
    13981 gcaatgagta acccgatcct gtcctggcgt cgcgtacggg cgctgtgcgt taaagagacg
    14041 cggcagatcg ttcgcgatcc gagtagctgg ctgattgcgg tagtgatccc gctgctactg
    14101 ctgtttattt ttggttacgg cattaacctc gactccagca agctgcgggt cgggatttta
    14161 ctggaacagc gtagcgaagc ggcgctggat ttcacccaca ccatgaccgg ttcgccctac
    14221 atcgacgcca ccatcagcga taaccgtcag gaactgatcg ccaaaatgca ggcggggaaa
    14281 attcgcggtc tggtggttat tccggtggat tttgcggaac agatggagcg cgccaacgcc
    14341 accgcaccga ttcaggtgat caccgacggc agtgagccga ataccgctaa ctttgtacag
    14401 gggtatgtcg aagggatctg gcagatctgg caaatgcagc gagcggagga caacgggcag
    14461 acttttgaac cgcttattga tgtacaaacc cgctactggt ttaacccggc ggcgattagc
    14521 cagcacttca ttatccccgg tgcggtgacc attatcatga cggtcatcgg cgcgattctc
    14581 acctcgctgg tggtggcgcg agaatgggaa cgcggcacca tggaggctct gctctctacg
    14641 gagattaccc gcacggaact gctgctgtgt aagctgatcc cttattactt tctcgggatg
    14701 ctggcgatgt tgctgtgtat gctggtgtca gtgtttattc tcggcgtgcc gtatcgcggg
    14761 tcgctgctga ttctgttttt tatctccagc ctgtttttac tcagtaccct agggatgggg
    14821 ctgctgattt ccacgattac ccgcaaccag ttcaatgccg ctcaggtcgc cctgaacgcc
    14881 gcttttctgc cgtcgattat gctttccggc tttatttttc agatcgacag tatgcccgcg
    14941 gtgatccgcg cggtgacgta cattattccc gctcgttatt tcgtcagcac cctgcaaagc
    15001 ctgttcctcg ccgggaatat tccagtggtg ctggtggtaa acgtgctgtt tttgatcgct
    15061 tcggcggtga tgtttatcgg cctgacgtgg ctgaaaacca aacgtcggct ggattaggga
    15121 gaagagcatg tttcatcgct tatggacgtt aatccgcaaa gagttgcagt cgctgctgcg
    15181 cgaaccgcaa acccgcgcga ttctgatttt acctgtgctt attcaggtga ttctgttccc
    15241 gttcgccgcc acgctggaag tgactaacgc caccatcgcc atctacgatg aagataacgg
    15301 cgagcattcg gtggagctga cccaacgttt tacccgcgcc agcgccttta ctcatgtgct
    15361 gctgctgaaa agcccacagg agatccgccc aaccatcgac acacaaaagg cgttactcct
    15421 ggtgcgtttc ccggctgact tctcgcgcaa actggatacc ttccagaccg cgccgttgca
    15481 gttgatcctc gacgggcgta actccaacag tgcgcaaatt gccaccaact acctgcaaca
    15541 gatcgtcaaa aattatcagc aggaactgct ggaaggaaaa ccgaaaccta acaacagcga
    15601 gctggtggta cgcaactggt ataacccgaa tctcgactac aaatggtttg tggtgccgtc
    15661 actgatcgcc atgatcacca ctatcggcgt aatgatcgtc acttcacttt ccgtcgcccg
    15721 cgaacgtgaa caaggtacgc tcgatcagct actggtttcg ccgctcacca cctggcagat
    15781 cttcatcggc aaagccgtac cggcgttaat tgtcgccacc ttccaggcca ccattgtgct
    15841 ggcgattggt atctgggcgt atcaaatccc cttcgccgga tcgctggcgc tgttctactt
    15901 tacgatggtg atttacggtt tatcgctggt gggattcggt ctgttgattt catcactctg
    15961 ctcgacacaa cagcaggcgt ttatcggcgt gtttgtcttt atgatgcccg ccattctcct
    16021 ttccggttac gtttcgccgg tggaaaacat gccagtatgg ctgcaaaacc tgacgtggat
    16081 taaccctatt cgccacttta cggacattac caagcagatt tatttgaagg atgcgagtct
    16141 ggatattgtg tggaatagtt tgtggccgct actggtgata acggccacga cagggtcagc
    16201 ggcgtacgcg atgtttagac gtaaggtgat gtaacttctt atctttcgcc agcaaagaca
    16261 ctaccgccgg gccggcaagg attgccagcc ctgcaattgc cagcaaaaag ttgttttcca
    16321 gtacgcggga caacagccac agcacaatca gcgctgcgcc aaaataccag gtcgtggtca
    16381 gctcttccaa cacgtctgcc acctcacgcc agcgttgctc ggggtggcgc tgaaacacca
    16441 acatcatcac taccgtgaca caatacagtg cgcataatcc cacgccgacg tgaaccagag
    16501 tcttactcat ccagcccacc accagcagcg ccactaccag taaatgcaac ataatctgcc
    16561 agcaacttag acccgttgcg acacgaacac gttgttgcca cttcatggct tctctcctga
    16621 agaatttatc tctacttact cagagtaccg aactttacgg aaaattccgt aagaccgcac
    16681 ctttttgtga cgccgactac actatttcaa tgagataaga ggcaaagaca ggaaaattat
    16741 gcccgatcaa acacaacaat tttcgttcaa ggtgctcacc atcaacattc aaaaaggttt
    16801 taccgcgttt aaccgacgct tcattttgcc ggaacttcgc gacgcggtgc gtaccgtcag
    16861 cgccgatatt gtttgcctgc aggaagtgat gggcgcgcac gaagttcatc cgctgcatgt
    16921 ggaaaactgg cccgatacct cgcactacga gtttctcgcc gacactatgt ggagcgattt
    16981 tgcctacggt cgcaatgccg tatacccgga agggcatcac ggcaacgccg tactgtcgcg
    17041 ttatcccatt gaacattatg agaatcgcga tgtttcggtc gatggtgcgg aaaagcgcgg
    17101 cgtgctctac tgccgcattg tgccgccgat gaccggaaaa gcgattcatg tgatgtgcgt
    17161 acatctgggc ctgcgtgagg cgcaccgtca ggcgcagctt gcgatggggt gaatgagcta
    17221 ccggacggcg aaccggtatt ggtggcgggt gatttcaatg actggcggca aaaagctaat
    17281 catccgttaa aagtgcaggc cggactggat gagattttta cccgcgccca cggacgcccg
    17341 gcgcgcacgt ttccggtgca atttcctcta ctacgactgg acaggatcta cgtcaaaaat
    17401 gccagcgcca gcgcgccaac cgcgttgccg ctgcggacat ggcgacacct ttctgatcat
    17461 gcccctttaa gtgcggagat tcatttatga aatgtagctg gcgcgaaggc aataagatcc
    17521 agttgctgga aaacggcgag caatattatc ccgcggtgtt taaggcgatt ggcgaggcac
    17581 aagaacgcat cattcttgaa acgtttatct ggtttgagga taacgtcggc aaacagctgc
    17641 atgcggcact actggcagca gcgcaacgcg gagttaaagc ggaagtcttg ctggatggct
    17701 acggttcgcc ggatctcagc gatgagtttg tcaataaact gacggcagct ggcgtagtgt
    17761 tccgctacta cgatccccgc cctcgccttt ttggtatgcg caccaatgtg tttcgccgga
    17821 tgcatcgcaa aattgtggtg atcgacgcgc gtatcgcctt tattggcggg ctgaattact
    17881 ccgccgagca tatgtccagc tacggtccag aggctaaaca ggattacgcg gtacgccttg
    17941 aagggccgat tgtcgaagat attctccagt ttgagctgga aaacctgcct ggacagagcg
    18001 cggcacggcg ctggtggcga cgtcatcaca aagcggaaga gaaccgccag ccgggagaag
    18061 cgcaggtatt gctggtctgg cgcgataacg aagaacatcg cgatgatatt gaacgccatt
    18121 atctgaaaat gctcactcag gcgcggcggg aagtgattat cgccaacgcc tacttcttcc
    18181 ccggctatcg atttttacac gccttgcgta aagcggcacg gcgcggggtg cggatcaaac
    18241 tgatcattca gggcgaaccg gatatgccga ttgtcagagt cggtgcgcgc ttgctgtata
    18301 actatctggt taaaggcggc gttcaggttt ttgagtaccg ccgccgcccg ctccacggca
    18361 aagtggcatt gatggacgat cactgggcga cagtagggtc cagtaatctc gatccgctca
    18421 gtttgtcact gaatctcgaa gcaaatgtca tcatccacga tcgtcatttt aaccagacgc
    18481 tgcgcgataa tctgaacggc attattgccg cagattgtca gcaggtggat gaaaccatgc
    18541 tgcccaaacg cacctggtgg aacctgacca aaagcgtgct ggcgttccac tttttacgcc
    18601 acttcccggc gctggttggc tggcatccgg cacacacacc acgtctgacg caggttgatc
    18661 cccccgcaca accgacaatg gaaacgcagg atcgggtaga aactgaaaac acgggggtaa
    18721 aaccctgatg agtaaatcac acccgcgcta gcgcttagca aagaagatcc tcacctggct
    18781 gttttttatc gcggtgattg tgttgctggt ggtctacgcc aaaaaagtgg actgggaaga
    18841 ggtctggaag gtcatccgcg actacaatcg cgttgcgctg cttagtgcgg tcgggctggt
    18901 ggtcgtcagc tatctgattt acggctgcta tgacctgctc gcccgctttt actgcggtca
    18961 caaactggcg aagcgccagg tgatgctggt gtcgtttatc tgctacgcct tcaacctgac
    19021 gctcagtacc tgggtcggcg gcattggtat gcgctatcgt ttgtactccc gactagggtt
    19081 accgggcagc actattacgc ggattttctc gctcagtatt accaccaact ggctgggcta
    19141 tattttactg gcagggatta tctttaccgc aggcgtggtg gagttgccgg atcactggta
    19201 tgtcgctcaa actacgctgc gtattctcgg cattggctta ctgatgatta tcgcggttta
    19261 tttgtggttt tgcgctttcg cgaagcaccg ccatatgacc atcaaagggc aaaagctggt
    19321 gctgccttca tggaaattcg ccctcactca gatgctgatt tccagcgtta actggatggt
    19381 aatgggggcg attatctggc tgttacttgg tcaaagcgtg aactatttct ttgtactggg
    19441 cgtgttactg gttagtagta ttgctggcgt catcgtgcat attccggcgg ggatcggtgt
    19501 gctggaagcg gtgtttatcg cactactggc tggggagcat acctcgaaag ggagcattat
    19561 cgccgcgctg ctcgcttatc gcgtcctgta ttactttatc ccgctgctgc tggcgctgat
    19621 ctgttacctg ctactggaaa gccaggcgaa gaagctacgg gcgaaaaatg aagcggcgat
    19681 gtgaaattta gttaagttct ttagtatctg cattcacgac taatgaaaaa aacgcgtatg
    19741 cctttgccag acaagcgtta tagctatcgc ggcaagtatc ccaaagaagc cgataaaa
//
LOCUS       DAJVTY010000068        19515 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVTY010000068 DAJVTY010000000
VERSION     DAJVTY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19515
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.068"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            6..81
                     /locus_tag="OQQ66_002148"
     tRNA            6..81
                     /locus_tag="OQQ66_002148"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OQQ66_002149"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OQQ66_002149"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="OQQ66_002150"
     CDS             complement(468..1226)
                     /locus_tag="OQQ66_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1905072.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /locus_tag="OQQ66_002151"
     CDS             complement(1234..2337)
                     /locus_tag="OQQ66_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1905073.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3517)
                     /locus_tag="OQQ66_002152"
                     /pseudo
     CDS             complement(2347..3517)
                     /locus_tag="OQQ66_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3585..4628)
                     /locus_tag="OQQ66_002153"
     CDS             complement(3585..4628)
                     /locus_tag="OQQ66_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1905074.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTALQSG
                     EVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAG
                     TDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNP
                     AEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPA
                     TPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNL
                     WNNGGIQYAPPVR"
     gene            complement(5059..5280)
                     /locus_tag="OQQ66_002154"
     CDS             complement(5059..5280)
                     /locus_tag="OQQ66_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905075.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5533..8637)
                     /gene="acrF"
                     /locus_tag="OQQ66_002155"
     CDS             complement(5533..8637)
                     /gene="acrF"
                     /locus_tag="OQQ66_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCS1905076.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            complement(8649..9806)
                     /gene="acrE"
                     /locus_tag="OQQ66_002156"
     CDS             complement(8649..9806)
                     /gene="acrE"
                     /locus_tag="OQQ66_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS1905077.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10205..10867
                     /gene="acrS"
                     /locus_tag="OQQ66_002157"
     CDS             10205..10867
                     /gene="acrS"
                     /locus_tag="OQQ66_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS1905078.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10870..11049)
                     /locus_tag="OQQ66_002158"
     CDS             complement(10870..11049)
                     /locus_tag="OQQ66_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1905079.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11133..12017)
                     /gene="yhdJ"
                     /locus_tag="OQQ66_002159"
     CDS             complement(11133..12017)
                     /gene="yhdJ"
                     /locus_tag="OQQ66_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1905080.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDII
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12103..12399)
                     /gene="fis"
                     /locus_tag="OQQ66_002160"
     CDS             complement(12103..12399)
                     /gene="fis"
                     /locus_tag="OQQ66_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1905081.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12425..13390)
                     /gene="dusB"
                     /locus_tag="OQQ66_002161"
     CDS             complement(12425..13390)
                     /gene="dusB"
                     /locus_tag="OQQ66_002161"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1905082.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13719..14600)
                     /gene="prmA"
                     /locus_tag="OQQ66_002162"
     CDS             complement(13719..14600)
                     /gene="prmA"
                     /locus_tag="OQQ66_002162"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1905083.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14612..16063)
                     /gene="panF"
                     /locus_tag="OQQ66_002163"
     CDS             complement(14612..16063)
                     /gene="panF"
                     /locus_tag="OQQ66_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1905084.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16053..16295)
                     /locus_tag="OQQ66_002164"
     CDS             complement(16053..16295)
                     /locus_tag="OQQ66_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1905085.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16388..17323)
                     /locus_tag="OQQ66_002165"
     CDS             complement(16388..17323)
                     /locus_tag="OQQ66_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1905086.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(17355..18185)
                     /locus_tag="OQQ66_002166"
     CDS             complement(17355..18185)
                     /locus_tag="OQQ66_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1905087.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVATQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            18528..19382
                     /locus_tag="OQQ66_002167"
     CDS             18528..19382
                     /locus_tag="OQQ66_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCS1905088.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         5170 a         4886 c         4670 g         4789 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtcttcc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggcccagt cttccgactg agccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggttc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacct
      781 gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttcttaggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta aaacggtcag atgagggaag agattgaaat gctgaaatac cattcccact
      961 tcttgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctacgaca
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaaccagaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta tggacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgttggc tataacgtga catgctgaaa caaaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaagcc gatgatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaccccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggtagg gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctcaagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaata ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccagttagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc ccagtgaccg agccgcttca
     2641 tgttgaccat aaggcactgc ctggatccca gcgcggataa tctcggcgat aaatgcagag
     2701 gtataaaccg aaagtgccag cgttaaggct gccagttcag gaattaaaac cattccgccg
     2761 cggaagttaa agcctcgtag ggctgggaca tcccagtgta gtgctgcgcc aaaaagccat
     2821 tgcgccagta aaggcaaacc gatgatcaac actgcggcga acggccaggt gcggcgcagt
     2881 tgtccggtct ttatctggta tgttttatta aagcggaata gcccaacaga aaggactata
     2941 gccataacaa cagccaggat aaacgcaata aatccgtctc ccagctgcgg tgacggaata
     3001 taaagcccac gattgctcaa aaaggccaga tcaaacgcgc tcacagcctg gcgcgtggtc
     3061 cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc gggggaatat
     3121 tacggaagat ctcaatataa attgtggata gctttcgtag cagtcagtta tccgaaagtc
     3181 tcgtcagacc gataaagaag cctaaaacag aagcgaacac aatacacaat gcagaaacca
     3241 gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc tgctggtaat
     3301 cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca aaacctgaag
     3361 taatgccacg attactgaga ttcgttacag tgttgtgaaa taaccaacca acaattccga
     3421 caacagcaac aacggcaagg atctggaata accaggcacg aaccgtaggg ttggcaaagg
     3481 agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa agcataacgc
     3541 tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg cacgggcggc
     3601 gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat tttcagcggg
     3661 ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg tttgatgatg
     3721 ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc tttacccagc
     3781 agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt ctgggaattg
     3841 atgcccatct cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat
     3901 tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac ctccggtaag
     3961 acaatccatt cagctgggtt gcttaatttg atgcgcaggg catacagttg cgactgatcc
     4021 gaggccagcg tatcgcagcg accagattcc agtgccttcg ctgattcgtc agagcgatcg
     4081 aaagtcaccg gtgtgtactt catattgttt gccttgaaat agtcggcaac gttgagttcg
     4141 gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc gctttttagc
     4201 cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc
     4261 attcccatcc cggcgtcgcg agatgaagtc caggtcgtat tacgggagag caaatccact
     4321 tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata tttcactttc
     4381 gtgtcgtcac caaatacagc agcggcaaca ctacgacaaa catcaacatc aacatcaaca
     4441 tcaacatcaa tacctgaaaa cttaccgtct gcatcggcat aagagaaccc aagtaatcca
     4501 tcactgatcc cgcattgcac aaaacctttt ttctgaacgg catcaagcgt cgcgccagca
     4561 tgcgcctgat ttgcaacggc aagcagcacg ctggcggcag ccagtgtggc tatcatcatc
     4621 tttttcataa tgcttcctgt gaggcgaaaa ttatcgttat aggacgtttg ggaaacgtct
     4681 tttacctgtc tgtggccatc ctcttataag caaaagtagt gccagctttt gcgcacgatt
     4741 tcgcgtcgca ataagctgag tgtagacagg aaataataaa aacagcgccc cgcaatggtg
     4801 cgaaaatgaa acatgcgcca cattttggcg caaaatattt acgcgtgttt tactaagcag
     4861 aataatgatt gttagcgtga gtatatttct ggtgggaagt gtgaaagtta aattgatgcg
     4921 ttatttcgaa agcagcgaaa taacgcgata taacatgcta aatcagcaga gaattagcca
     4981 gcagaatata gcaatatttt atcttatgta aagatactgt atcgttaaac agagcgcgaa
     5041 actccgcgcc ctgttcagtt atttcgacag agcgatgatc cccatcgtca ggccagcaac
     5101 agccgctgcc ccgccggcaa tagccccagc cgtttcccag ttatcctgag tcgtgccttt
     5161 catacaggta ttggtgtgaa ccagacaacc ctgatcgtca taaaccggaa cgcagggcga
     5221 atcgtgagca cagcccgcca gcaatgcggt gagcaatgcc acaggaatta atcttttcat
     5281 ggtgttacct cagtaataaa cgctaacgct taataagtta actctcttat taacgagctg
     5341 aatgccattt tatcaccgga ggttataaat attgcggaat gaataatgac aaattatgtg
     5401 aattgtaaaa atagtgaagg gagcgggaac tatagaaagt ttttaaagag tgagtggaag
     5461 aatatcttat taatttttat gggtttcact gaaaataata aaggcacccg aaagtgcctt
     5521 tatgtttctg atttatcctt taaagcaacg gcggatcacc acaaagaaca ccggtaggaa
     5581 gaagattgcc agcaacgttg cagagaccat tccgcccatt accccgatac ccactgcgtt
     5641 ctgcgcgcca ctgcctgcac cgttactgat agctagcggt aatacgccga gaataaaggc
     5701 gagagaggtc atcaggatag gacgcagacg catacgtact gccattagtg tcgcttcaac
     5761 aacaccttta ccctctttct ccatgagatc tttagcgaac tcaacaatca aaatagcgtt
     5821 tttggccgac aagccaattg tcgttagcaa gcccaccata aagtagacgt catttttttg
     5881 attaaagagt gtcgccgcca gcagcacgcc gacaatccct aacggcacga ccaacataac
     5941 cgagacagga attgaccagc tttcatagag tgcagcaagg cacaggaaaa caaccacaaa
     6001 ggaaattgct actagagcgg gagcctggtt tcccgataag cgttcctgat acgacatacc
     6061 cgtccagtca taaccaatgc ccgcaggtaa ttttgacgca aggttttcca tcaacgccat
     6121 ggcatcgccg gaactggttc ctggcgcggc ttccccctga atctccattg acggcagacc
     6181 gttgtagcgt tccagtcgcg gagagccata cacccaatgt gaagtggtaa aggccgagaa
     6241 tggcaccatt tcgccgttgg cgctgcggac ataaagttta tcgacatctt ctggcagcat
     6301 acggaatttg gcatccgcct gaacatacac ctttttcacg cgaccacggt cgatgaagtc
     6361 gttaacgtaa gtcccaccca acgccgttga aatggtctga ttgatgtcag aaagtgaaac
     6421 acttaatgcc tgcgcctttt cctggtcaac ttccagttta aactgcgcgg tgtcttccag
     6481 gccattagga cgcacgctga ctaagctggc aggatgttgc gccgccatac caagcaactg
     6541 gttacgggcc tgggttaggg catcgtgacc cagcccagcc tgatcaatta actcaaagtc
     6601 gaaacccgtt gccgtgccca gttcaacaat ggctggcata ttgaatggaa tgacaaaacc
     6661 gtcgcggatc ttgcccaatt ccattttggc acgatggatt accgcttccg cactgttttc
     6721 atcaccatta cgctcttccc acggtttcag actgacgaag gccataccgg cgttttgtgc
     6781 ctggccgctg aagctaaagc cgttaaccgt aaagacactt tcaacgttcg ctttctcgtt
     6841 cttcagatag taatccgtca cttgatccaa cactttttgc gtccgctctt gcgtcgcgcc
     6901 agcgggtaac tgaatcatgg tcagaaagac accctgatcc tcttcaggta agaaggaaga
     6961 cggaagacgt aaaaacaaca ccaccattcc tgcaacaatc agcgcataga tcagtaaata
     7021 tcgtcctgtg gaaccgagga ttttgccgac gctgttggtg tagtggttaa cgctatgatc
     7081 gaaggtggta ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct cagcagagac
     7141 gggtttaagc agcgttgcac ataacgcagg ggtaagaatc aatgccacca gaacagaaag
     7201 tgccattgcc gaaacgatgg tgatagagaa ctggcgataa attgccccag tagaaccgcc
     7261 gaagaatgcc atcggaataa ataccgctga cagcaccatc gcgataccca ccagtgcgcc
     7321 ctgaatttgc gacatcgatt tttccgtcgc ttcttttggc gggagcttat cctccatcat
     7381 cacgcgctcg acgttctcca ccaccactat cgcatcatcg acgagcagcc cgatggcaag
     7441 caccatcccg aacatcgtta gtgtgttgat ggagtaacca aaagcggcga ggatggcaaa
     7501 cgtccctaac aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg
     7561 caagaacaga tacatcacca ggaacaccag cataatggct tcgaacagcg tttttaccac
     7621 ttcgtgaata gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc
     7681 ctgcgggaag aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag
     7741 agcattcgcg ccggttgcca gcttaatccc caggcccgcc gccggttttc cgttgatacg
     7801 agcgataacg ttatagtttt caccgccaag ttcaacccgt gcgacatctt tcaggcgtac
     7861 caccgagccg tcactgttta cgcgcagggt cactttgccg aattcttccg gattttttaa
     7921 ccgcgtttga gcaataatcg aggcattcaa ctgttgccct ggtaacgctg gcgttccgcc
     7981 caactgtccg gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg
     8041 tgtcagttta tatttgttta gcagatcggc gtccagccag atacgcatcg catactgtgc
     8101 gccgaaaagc tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga
     8161 ggccacatag tccgagatat cgtcctgtgt ggtgcctggg ttatcagaga caaaacccgc
     8221 caccatcaaa tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg
     8281 cggcagcaac ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg
     8341 atcggtcccg gactggaagg taagggtaat tgtcacgcta ccggcggaat cgttggtgga
     8401 ggacatatac atcaggttat cgataccatt catattctgt tcgataacct gcgtcaccgt
     8461 atcctgcacg gtctgcgcat cagcacctgg atagtttgcc gaaacagaaa ccgcaggcgg
     8521 tgcaatggtt ggatactgag cgacgggcaa ttgtaggatc gccagtgcgc ccgccatcat
     8581 cagaataatg gccagcaccc atgcaaatat cggtcgtcga ataaaaaagt ttgccatgtc
     8641 agattacctt acttcgatgc agtatctgcg ggggtatcgg tagtggcttt aacctgctct
     8701 cccggacgcg ctttttgcag gccgctgaca atgacttgat cgccagattt cagaccttcg
     8761 ctaatcaacc atttatcgcc aatcgcctga ctggcaacga cagggcgcgc ttccacctga
     8821 cttttatcgt taacaatcag cacggttgca tcaccacgcg gtgtgcggct aacgccttgt
     8881 tgcgggataa gaatggcgtc aggttggacg ccttcatcaa tccgtgcacg cacatacata
     8941 cccggcaaaa gcgtatgttg cgggttaggg aagacagcac gtagggttat ggagccggtg
     9001 ctttcatcaa cggtcacatc agagaattgc agcgtacctt tcaggggata ggtttgaccg
     9061 ttttccatga ccaactctac gttgctggtg gcgttttcct tatgcaaatt tccttgctct
     9121 acggattgct tcagcctcat aaaatcgttg ctggattggg tcacatcaac gtagatagga
     9181 tcgagctgct ggacagtcgc cagttcagtc gtttgcccat tagtgacaag agcgccttcg
     9241 gtcacggtcg atttgccgat acgtccgcta attggcgcag tcactttggt ataagcgaga
     9301 ttgatgcgag cgctttcgac tgtgcctttt gcggcaatca cggcggcatc ggcctggcga
     9361 gcatcagcaa tggcctggtc gtactcctgc tggctgatgt atttcgtacc cacgagcgga
     9421 acgtaacgtt ttaccgtcaa atgcgcgatg gcggcggcgg cttcactttt cgccagttcg
     9481 cctttcgcgc tgttataatt tgcctgatag gtcgcgggat cgatctggta cagggactgg
     9541 cctgcttgta catcgctgcc ttcagtgaaa ttacgattca gtacgatccc gctaacctgt
     9601 gggcgaactt cggctatacg gtaagcattg gtgcggcctg gtaattcagt cttaacttct
     9661 aacggggccg ttttcacaat atgaacggta acctgcggtt caccgacgtg agctttctct
     9721 tctcccttat cgttacaacc ggcgattaaa gccgcggaga tcagaataaa ggagggcagg
     9781 aggaaaaacc tggcatgttt cgtcattact attcctcaaa aaaccaaaag cgcgttattt
     9841 acccagaaag gcaacgcgtt aactcgcaga aagaaaaata cagtttgcta tcctacaaat
     9901 tatcattcgt cgatgtaagg aatagttatg aatactggcg tctcaaggca cataaacaca
     9961 aaaaaagatt aatattctac tgttttattt tgacgcgggt tgaaagaggc agaattaaaa
    10021 cctcgtaaat tgaaatatat attcatgtgg cgaatgtatt ttaggtaaat aatatacatt
    10081 gtttatttga cgatttatag caactcaatg ttaccctctc gtataaatac acattagatg
    10141 atagattaac cttcgctatt ttctcaccct gtgtcgaata tatttatttc ctgaataatt
    10201 aatcatggca aaaagaacca aagccgaagc tctgaagacc cggcaagaac tgattgaaac
    10261 tgccatcgcc cagtttgcgc agcatggcgt aagcaagacg acgctcaacg acattgccga
    10321 cgccgctaac gttacgcgtg gcgctatcta ctggcacttc gaaaacaaga ctcaactgtt
    10381 taatgagatg tggttgcaac agccttcatt gcgggagtta atccaggaac acttgacggc
    10441 tggattagag catgacccgt ttcaacaatt gcgtgaaaaa ttgattgtcg gcttgcaata
    10501 tattgccaaa attccccgcc agcaggcgtt gctgaaaatc ttatatcaca aatgtgaatt
    10561 taatgatgag atgctggccg agggagtgat acgcgaaaag atgggcttta atccgcagac
    10621 tctccgcgaa gtattgcagg cgtgtcagca acaaggttgt gtagcaaata acctcgattt
    10681 agatgttgtg atgattatta ttaatggtgc cttcagcgga attgttcaaa actggttaat
    10741 gaatatggcg ggttatgatc tttataaaca agcacccgcg ctggtcgata acgtattaag
    10801 aatgttcatg ccagatgaaa acataacgaa attaattcat caaacgaatg aattaagtgt
    10861 catgtaaatt cacattctgt cctgaaaatt cagttcgtca cagttaacca gttttagtgg
    10921 attaactgag ttcatattac ggcatttgtc tgtttctgtt gtcagcagtt caatccactg
    10981 catcagtaaa gtatcaaaca gaaaaacgaa gacagcgaaa acgacgagcc accaatactt
    11041 gcgaatcatt acctgagcct gtgcatgttt aaccaagtct ggcaggaaat atacacgaaa
    11101 tgaataatct gaaaaccagg cttacacttc ctttactttg caatgagatc ggggtcaact
    11161 tcgcttaacc ggcttcgttt tgacaggttg ccggtctttc tttttttcac tttcgccagt
    11221 tcttccgcag agtaatgcga cgcgacatcc agccgtcgaa gccccatttt gatgtactcg
    11281 ctgttgatct caataccaat gaattttcgc ccgctggaga cggctacggc accggtagta
    11341 aagctaccgg caaacgggtc gagaatgata tcgcctgggt tggaagaggc gagaataatg
    11401 cgtttcagta aggcttccgg tttttgcgtc gggtggttct catattcatc catcaaataa
    11461 cgcacgcgcg gaaaatccca gacgttaccc ggtacttttt gatgattgta tggctgtgga
    11521 ggatttttgc gataatcgat caacgcgcgc tgcgatccgg ttttggcttc taccagaata
    11581 gcatcaccgt tgaatgtgta gttctttgcg tctttcacca tcatcaggat ggggtcgtac
    11641 atggagccgt agtgtttttt cgcctgtact ccagaactgt catatgacca gacgatgcga
    11701 cttttgatgg taaaaagctt gcggcactgg agatcgataa agggcatgtt ttccgtgctg
    11761 ttcatgatgt acatgctgcc ctgctttttc agaacgcggt ggcactctgc aatcacttca
    11821 aacagccagt cgataaacag atcttctttc caggcttcga tcagaccatc aaaattttta
    11881 ccgatgttat atggtgggtc ggcaaagatc agatcgacac tttcggcggg gatcttttta
    11941 agttcggcaa gcgcatcacc gtgaataatg gtcttagctt cattaccaaa ccgggtcggt
    12001 tcacatcctg ttctcatggt cactcccttt gtgacaccta taaaaaaggc gcttccccat
    12061 gccgagtagc gcctttttaa tcaagcattt agctaacctg aattagttca tgccgtattt
    12121 tttcaatttt ttacgcagcg taccacggtt gatgcccatc atcagcgcag cacgggtctg
    12181 gttaccacgg gtgtattgca tcaccatgtc caacaggggc tgttctactt cagccagtac
    12241 cagctcatag aggtcattca catcctgacc attcagttga gcaaaatagt tcttcagtgc
    12301 ctgtttaacc gagtcacgca ggggtttttg ggttacctga tcctgagagt taacggtaga
    12361 aacggtcagt acgtcagaat ttacgcgttg ttcgaacata gttctgtcag ctctttattt
    12421 ctgtttacgc aaaattttcg aagtatgcct ccaacgcctc cagctgttcg ctggcatcct
    12481 caatggcgtt gaatgtgcgc cgaaactggt catttggagc gtgttcctgg agataccagg
    12541 aaacgtgttt acgtgcaatt cggtaccctt ttgccggacc ataaaagtca tgcagttccc
    12601 gaacgtgcgc gcaaagcaag cgcttaacct ctgccaaagg cagtgggggc agcaactccc
    12661 cagtgtccag ataatgctgg atttcccgaa agatccaggg tcttccctga gctgcgcggc
    12721 ctatcatcag ggcatccgcc cctgtatagt cgagcacagc tctggcttta agcgggtcag
    12781 taatgtcgcc attcgcgata accggaatgg aaactttctg cttaactgcc cgaatactgt
    12841 cgtactcagc ttctccattg aacaaacagg cgcgtgtacg gccatgaatg gtcagagcct
    12901 gaatgccaca gtcttcagcc agttgggcaa tctcttcgca gttacggtgt tccggtgccc
    12961 agccagtgcg aatcttcagg gtaacaggaa cgtccactgc attgacgacc tcggtaagga
    13021 tcgatttaac gacatccggg tactgcaaga gggctgaacc tgcgagcttg cgattcactt
    13081 ttttagccgg gcaacccata ttgatatcaa taatctgggc accgctttcc acgttaatac
    13141 gtgctgcatc tgccatttct ttcggatcgc taccagcaat ttgcacggtg cgaataccgg
    13201 gttcatcaat gtgcaccatc cgtaaacgag atttgtcgct ttcccaaacc tgtgggttag
    13261 aagacatcat ctcggatact gtcaatccgg ctcccatctc gtagcacaac gtccgaaaag
    13321 gtctgtctgt aatgccagcc atgggcgctg cgatcaggcg atttctgagc tgatattgtc
    13381 cgatgcgcat gagttaagaa atgaccatac tgtgactgca aggcggcgta tattacgcat
    13441 tttttgcacg agatgaaagg ccaaactttg accaatcctc tgagatggat caaagaattg
    13501 catttaaaat gagcgtggtg cgataattac tcataaaaat catcatatta gaaaatagtg
    13561 actaaaattt acattcaaag aaatttgagt aagttctcaa tttttcttta tgaatgaaaa
    13621 ttttggcacg caaatttgcg taaataaccg gcaaatttac gtgccttttg tgagcttgct
    13681 cgcacttcgc cccgcgtcac cctacggcga tgcgaaggtt aattcttacg accggtaata
    13741 cggcaccact cttctttttc cacgaccggg tccagtgcga agctatcggc ataagcttca
    13801 caaacgctct ctgcctggct tgccagaata ccggaaaggc ccagcaaacc gcctgaaacc
    13861 ggcaggacgc tgattaacgg tgccagttca cgtaatgggc ctgcaaggat gttcgcgacc
    13921 accacgtcgg ctttcatttc ttctggctga tctttcggta agtagagttc cagacggtca
    13981 gaaacgccat tacgttcggc gttgtcgcgg ctagcctgaa tcgcctgcgg atcgatatca
    14041 ataccaatgg cttttgctgc acccagtttc agcgccgcga tagccagaat gccggaaccg
    14101 cagccaaagt cgatgactgt tttaccagtt aaatcgaggc tgtcgagcca ttgtaggcac
    14161 agagaggtag ttggatgggt acccgtacca aacgccagcc ctgggtctaa catcacgttg
    14221 acggcgtttt cgtccggcac atcacgccag cttgggcaga tccacagtcg ttcaccaaag
    14281 cgcatcgggt ggaaattatc catccattcg cgctcccagt ctttatcttc cagttgttcg
    14341 attttatgcg cgaagcctgc gccgagcagc ggatggtttt ccagaatcgc caccacgtcg
    14401 ttcatatcgg tttcagcgtc gaacagacca atcacatcgg tgtcgcccca caggcgcgtt
    14461 tcgcccggca gcggctcaaa tactggcgta tcgtgggtat cctgaaaagt gatagaaacg
    14521 gcacccgctt ccatcagcgc atcgctaaga tcttcagcgt tcgcgccggt ggtgttcagt
    14581 ttcagttgga tccaaggcat ggcaaaactc tttatttatc agtagtcaaa acggtagctt
    14641 gcgggacgga tgtaccgaaa cggtttccga ccaggaaagc cagcaaactt agtagtaacg
    14701 agggcacgat agggtggaag cccaggtact gaatattcag cgtcgcgagt acggcataca
    14761 gcacgccgcc aacgatcatc gcacttagcg cgcctttggc gttggcgcgt tcccagtaaa
    14821 gacccagcac cagcggccac aggaaaacgg cttccaaccc accgaaggcc agcaaattca
    14881 gccatatgat catttctggc ggcttccagg cggcaagcag cagcaacgcg ccgagaacta
    14941 acgtaattac cgccgacatc cgcttcagac gcgtctcgtt ttgcatttga tccggacgga
    15001 tattcagata gagatcttta atgatcgtag cggaactttg tagcaattgg gcgttaattg
    15061 tcgacatgat cgcagccata ggtgcagcca gaaagatccc ggcagcaaac ggtggcagca
    15121 cttttaccat taacgtcggg atcaccagat ccggtacggt gagatcgggg atcactgcac
    15181 gacctaacgc tccggccagg tgcataccga acatcaggat tgcgactaca atcgtaccga
    15241 tgatgatccc ccggtgtacc gctttgctgt ctttataaga gatacagcgc accgcagtat
    15301 gcggcaggcc aatcacgcca aagcacacca gtacccagaa cgacgtcata aaggcaggcg
    15361 acagaatatc gtcagcgcct tgtggcgtaa ccagttgcgg atcgatggtt tgcagcgtct
    15421 ctaccgcatt acttaaacca cctgcggcat gtaccacgcc aataagcagc acaacggtgc
    15481 caatcagcat cacaagccct tgcatggtgt cgttcagcac gctggcgcga aagccgccaa
    15541 aggcggtata caacgcaatg ctgataccga aaatcagcag cccggtttca taaggaatac
    15601 ccgccgcggt ttccagcagg cgcgcaccgc cgataaactg cacggtcatt gccccaacga
    15661 aggcaaccag caaactcaaa ctcgccagcc acaccagaag acgactctgg tagcgggcaa
    15721 acagcatgtc gttcagcgtc actgcattgt agcggcgcgc aagaatcgcg aacttcttgc
    15781 cgagaatacc gagcgaaagc cagactgcag gaagctgaat catcgccagc aatacccaac
    15841 ccaggccgta tttataagca gctccaggcc cgccgataaa cgaacttgca ctgatatagg
    15901 tcgcggtgag cgtcatcgcc agcacaatac cgcccataga gcggctgccg aggaaatact
    15961 cattaaggaa ggtaccggtg ctccgtttac gcatcgcata aacagagata ccgaacacca
    16021 ccaccagata ggcgaccagc ggtagaatca cttcaagctg catcgtcatc ctccagtggg
    16081 atatcgcgat agataaattt caccatcgcc cagcacagtc caataaacag cagcggcgtc
    16141 aggatgcagg ccatctcaaa ccagcgcgga aagccggtaa aaccgggggc aacgtcaggt
    16201 aagtaagcgg ctgctaacca aactgccaga tacaaaaggg tcagccccag cgcccagcgc
    16261 gcctctttat gggcctgaac aaaacgagtg tccacggaag ggttcctcct catgggggga
    16321 tacgagaaac gggcaattct acggcaactc ttcaggcagg gaaaataatc tgacagaaaa
    16381 ttgccgttca gtgctgctgc aaaaagtctt caatgtcagc cagtgaggat atcccttcca
    16441 tcggaccttg ccgcattacg gcgagcgcgc cgctggcatt tgcatattgc agtgccttat
    16501 gtgccgggaa accggataag aaaagagtga taaacgttgc gccaaagcag tcgcctgcac
    16561 ccgttggatc gataatttcg atatcgtggc ctgtaacatg ctgggcgtgt aatgtaccgt
    16621 ttttaagctt gtaatagctg gccccacgct gggcgcgttt tatcgccaca tgttttacgc
    16681 cgccgtggag aagatcgcta acaatctgtt cttctgacag atttttgtga cgcgcgaaga
    16741 aagggagttc gctttcgctg gggatgaaaa tatccgtata ttcaatcaaa taatcgagag
    16801 cctgcgccat ttcaggaatg ctcagcatct ctttgcgaat attgggatcg aaagaaacgg
    16861 tgccgccagc cgatttgatc gtcgttattg ctttacgcat gacatcgatc atgcgaaatg
    16921 agaacaatga tgagcccaca atatggagat ggttacactg ttttagcaga tccttatcaa
    16981 tgtgctcagc agtaaacaag ccgcaggcgc tgttagggat attaaagacg aaatcgcgct
    17041 gctggggatt ttgataactc acgaacgccg tacctgtgac ggcattgttc ataacatgga
    17101 tcccatcggt aatgacacct tcatgacgga ggcgctctat aatgagtcgg ccaaaatcat
    17161 cattaccaac acatccgaag aagatggccc gatgggacag ttttgccacc tgagcagcaa
    17221 aaattgctgg cgcaccgctg ggaaatggcc cgataaactc cccaggctga gtgaattttt
    17281 gatgtgggtt gcgggacaaa aactcggcca gcaattcgcc catagcgcag atagttattg
    17341 tcacaaaacc cctctcattc ctgaagcctt tcgcgattgg accaggtaaa gcactcatta
    17401 ctggtataaa ggataacgaa atcagccgac tgcaattgtt gctgatattg ttcgttaatg
    17461 ctgcggctga cgatcacatt ttctgcctga tgcaaatgcc cgatagcagc cgttgatggc
    17521 gcaggatcgg cggcaacgat aaccgtctta ttctgggcat gatcgaaaca gagctgccag
    17581 gcagcggcca gtctgggaac gttgatattt aaagtaccat cagcatccag atggttgatt
    17641 tccagaatgc tatgtgtaat ggtgaaatgc tgaagtgcct gttcaagcgg ttttccggcc
    17701 agtgcggtgt cggggcgcag catttcactg ccagccagta ttatttcgcc atcaataaac
    17761 tgatttgcca gcagcgcatg ttcgggatcg ttggtgatca gacgcagttt tttcacttcg
    17821 ctgagcaatg gaatgatctt acggcagatt gtcccatgtc caagaaatag cgtgtcacga
    17881 tcgcgaatta accgtgcgca atgtcgtgct atttccattt gttgtgccag agacaatggc
    17941 gcgatcggtt gtatcgcttc tggttcagag gagagtggtg tcgcaatagc accgccaaat
    18001 gatcgcttta aatatccctg ttgctccaga taactgagat cgccgcggat cgtcacgcca
    18061 gagacaccaa gttgcgcaga taagtcatca actttgattt caccttgggt ggcaacaatt
    18121 tctgcaattt ttaatctgcg tttcagtagg ttagacgatt ttctcggcac gttaacactc
    18181 ttcattggcc tttaatgcgt aacactatag ccccttgtat ttttctttga aagttatcct
    18241 tcaataatat cgacaattcg aaaagaaaat catgggtcaa aaatcgcaat aaaaatagat
    18301 taattcattg aaaaataaat gaatttattc atatttatgc agagatgtta ttggtttcac
    18361 ttgtaaagtg aaaaacaaat taagttattt ttttgtaagt tattttgatg atattcgaca
    18421 tgtatcacgc tttttgccgc ttttgctgtg ctagtttgct ttcaaaatga aagtgaatat
    18481 tctctttctt cattttgtaa ggcgaacgac aaataacagg agctgcgatg tacatcattt
    18541 caagcaaaaa catgctgaaa aaagcccagt cagagggata tgccgtcccc gctttcaaca
    18601 ttcataacct tgaaacttta caggtagttg ttgagagcgc tgcacaactt cgctctcccg
    18661 ttatgctggc gggaacaccc ggtacttatc gttatggtgg cgtgggaagt ttaatttcgt
    18721 tagtacaatc gctggctcgc gagtacaacc tgccgttagt cctgcatctc gaccatcatg
    18781 aagagagtga tgacattttt aacaaggttc gcgcagggat ccgttctgtc atgattgatg
    18841 gctcccattt gccatttgaa gacaacattg cgcgagtgca agaagttacc accttttgcc
    18901 atcgctttga tgttagcgtt gaggccgagc tagggcgtct tggcggccag gaggacgatc
    18961 tgctggtgga tgaaaaagat tccgcttata cctcaccagc cgcagctgca gagtttgttc
    19021 aacgtaccca aattgactcc ctggccgtag caatcggcac agcacatggt ttatatgcgc
    19081 atgaaccacg actggatttt gtgcggctgg aacagattcg tcagcgagtg gagattccac
    19141 tggtgttgca tggtgcttcg ggcttatcag aacaggacgt tcagcactgt attcgccgcg
    19201 gtatttgtaa agtgaatgtc gcgacagaat tgaaaattgc gtttgccgat gcgttaaaag
    19261 gatatttcta caacaatcct gcggcaaatg atccacgcca ctatatgcag cccgcaaagg
    19321 ccgccatgaa agaagtcgta actcgtatta ttggtgtctg tggtagtgaa ggaaaaattt
    19381 aagacgttgt accctacaaa aacaatgagg attgtttatg aaaaatatta aattacttgg
    19441 ttcagctatg ttggtgagcc tggcattgtt ctcgcaaaat tcgttggcaa aagagtataa
    19501 aattggtgcg tcatt
//
LOCUS       DAJVTY010000069        19496 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVTY010000069 DAJVTY010000000
VERSION     DAJVTY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19496
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.069"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..840
                     /gene="wecH"
                     /locus_tag="OQQ66_002168"
     CDS             <1..840
                     /gene="wecH"
                     /locus_tag="OQQ66_002168"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1905089.1"
                     /translation="SVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIAL
                     FTSINMELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGII
                     ANPNTVPQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFA
                     TGVFIISRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLI
                     SRHSLGIYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNR
                     LVS"
     gene            complement(882..1319)
                     /locus_tag="OQQ66_002169"
     CDS             complement(882..1319)
                     /locus_tag="OQQ66_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1905090.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1365..1706)
                     /locus_tag="OQQ66_002170"
     CDS             complement(1365..1706)
                     /locus_tag="OQQ66_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905091.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(1871..2062)
                     /locus_tag="OQQ66_002171"
     CDS             complement(1871..2062)
                     /locus_tag="OQQ66_002171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905092.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(1995..3314)
                     /gene="xylB"
                     /locus_tag="OQQ66_002172"
     CDS             complement(1995..3314)
                     /gene="xylB"
                     /locus_tag="OQQ66_002172"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1905093.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHSFCH
                     ALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLSGER
                     TPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTLIGG
                     GARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3431..4708)
                     /gene="xylA"
                     /locus_tag="OQQ66_002173"
     CDS             complement(3431..4708)
                     /gene="xylA"
                     /locus_tag="OQQ66_002173"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1905094.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5074..6045
                     /gene="xylF"
                     /locus_tag="OQQ66_002174"
     CDS             5074..6045
                     /gene="xylF"
                     /locus_tag="OQQ66_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1905095.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEIKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6123..7664
                     /gene="xylG"
                     /locus_tag="OQQ66_002175"
     CDS             6123..7664
                     /gene="xylG"
                     /locus_tag="OQQ66_002175"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1905096.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7642..8823
                     /gene="xylH"
                     /locus_tag="OQQ66_002176"
     CDS             7642..8823
                     /gene="xylH"
                     /locus_tag="OQQ66_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1905097.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            8901..10079
                     /gene="xylR"
                     /locus_tag="OQQ66_002177"
     CDS             8901..10079
                     /gene="xylR"
                     /locus_tag="OQQ66_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCS1905098.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10187..11011)
                     /locus_tag="OQQ66_002178"
     CDS             complement(10187..11011)
                     /locus_tag="OQQ66_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1905099.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11331..13361
                     /gene="malS"
                     /locus_tag="OQQ66_002179"
     CDS             11331..13361
                     /gene="malS"
                     /locus_tag="OQQ66_002179"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1905100.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13539..14792
                     /gene="avtA"
                     /locus_tag="OQQ66_002180"
     CDS             13539..14792
                     /gene="avtA"
                     /locus_tag="OQQ66_002180"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1905101.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(14943..15416)
                     /locus_tag="OQQ66_002181"
     CDS             complement(14943..15416)
                     /locus_tag="OQQ66_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1905102.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15518..16366)
                     /gene="yiaJ"
                     /locus_tag="OQQ66_002182"
     CDS             complement(15518..16366)
                     /gene="yiaJ"
                     /locus_tag="OQQ66_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1905103.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16567..17565
                     /gene="yiaK"
                     /locus_tag="OQQ66_002183"
     CDS             16567..17565
                     /gene="yiaK"
                     /locus_tag="OQQ66_002183"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1905104.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            17577..18045
                     /locus_tag="OQQ66_002184"
                     /pseudo
     CDS             17577..18045
                     /locus_tag="OQQ66_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            18163..18636
                     /gene="yiaM"
                     /locus_tag="OQQ66_002185"
     CDS             18163..18636
                     /gene="yiaM"
                     /locus_tag="OQQ66_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCS1905105.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            18639..>19496
                     /locus_tag="OQQ66_002186"
     CDS             18639..>19496
                     /locus_tag="OQQ66_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1905106.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQ"
BASE COUNT         4934 a         4886 c         4737 g         4939 t
ORIGIN      
        1 agcgtgccgc tatttttcat gatttccggc tatctctttt ttggcgaacg cagcgcccag
       61 ccgcgccatt tcttgcgtat cggcttatgt ctgatttttt atagcgcaat cgcactgctc
      121 tacattgcgc tgtttacctc catcaatatg gagttagcgc tgaaaaacct gctgcaaaag
      181 ccagtgtttt accacttgtg gtttttcttc gcgattgcgg tgatttatct ggtttcaccg
      241 ctgattcagg tgaagaacgt cggcggaaaa atgttgctgg tgctaatggc ggtgattggc
      301 attatcgcta acccaaacac agtgccgcag aaaattgacg gttttgaatg gctgccaatt
      361 aacttatata tcaatggcga tactttttac tacattctgt atggcatgtt gggccgcgct
      421 atagggatga tggacacaca gcataaagca ctgtcgtggg tgagcgccgc gctgtttgcg
      481 acgggggttt ttattatctc tcgcgggaca ttatatgaat tgcagtggcg cggaaatttt
      541 gccgatacct ggtatcttta ctgtgggccg atggttttta tctgcgcaat cgcgctattg
      601 actctggtta aaaacacgct ggatacgcgt accattcgcg gacttggctt aatctcccgc
      661 cattcattgg gtatatacgg attccacgcc ttgattatcc atgcgctgcg cacccgggga
      721 attgagctta aaaattggcc aatactggat attatttgga tcttttgcgc gacgttggca
      781 gcgagtttgt tactttctat gctggtacaa cgaatcgaca gaaacagatt agtgagttaa
      841 gtaaaagccc gttcacattg gactgaccgg gcttacgtga attattcgct gtattcttcc
      901 tgggtaacct gtgtttcttt tggcgggttt attccggcat cacgaatatt cttctgcacc
      961 gctactgcac caaacaagct taagaagaaa gccagtccat aaaaaccttt ttcactgagt
     1021 aataatgtcg cattccacag acctaccatc agtaatgcaa cagagataat aaagacagtc
     1081 aggcaggtca tataataaat ggaagtggtc ggtatgcctt catacttgtc ccgaacggtc
     1141 ttttgataag acgcagcaga aaacagtcct aacaccagta cggcaaaata ataccctttt
     1201 tcatttaact gcatctctgc attccatagc cctaacagat aggtaacgat accaccaacg
     1261 agagctatcc atgacacaat actaaaggcc ggtgaatagg ttgatatttt gttgtccatc
     1321 acagtattcc tttcattcct gaatgtgtaa gagctttatg ttgctcatac cgagcgaaac
     1381 aaacgcattt gaccaaacaa gcagacaaaa aaggcgatgg ggtagatcac catttcataa
     1441 gcattgatag gtgcatttaa aacaccgata aacaagagac cgatagtgat taacgcaact
     1501 aactggcaga tatgggtaaa aaaattatcc agttgcccga gtttttctgc gcgaagatat
     1561 gcatagttac caaaagttgc tgtcattaac acgccaagaa aatagccttt tccacttagc
     1621 aatgggcatg agatccatag cccaaccaga taaaccagcg ccccgacaac aaataatagt
     1681 tttgccacag tttttgaggt tttcataacg atctccatat ctaccagcga tacattacga
     1741 gtaaccaacg aaagacaaaa ctgaaaaatg ccattaacaa atgattttca gaataaattc
     1801 atactaaata ttaattagtg agatatatag atgtgaatta tcccccaccc ggtcaggcag
     1861 gggataacgt ttacgccatt aatggcagaa gttgctgata gaggcgacgg aacgtttcac
     1921 gtcgtggctg ataagcggca taacgctgcg catctggtag atgcgactgt tctaacggta
     1981 gctgcggcaa caactcaatg agcgatttct ctggattcgc cgcaatctgc gccagccttg
     2041 ctgcgcccag tgctggcccc acatcccccc gtacggtaat cgagctgctg accgctgata
     2101 tccgccagca tctgacgcca gtactcacta cgcgccccgc caccaatcaa cgtaacactt
     2161 tgcggtttaa tgccgcaggc atgcacgaca tccatgccat ctgccagcgc ataacccacg
     2221 ccttccagca ctgctcgcgc cagttcattg gggccatgtt gatgagtcaa accaaagaaa
     2281 acccccttcg cctggggatt attgtgtggt gtacgctcgc cggaaagata aggcagaaac
     2341 caaactggct cggcactttc atcagcctgt tgagctgcag cgattaaagc tgggacattg
     2401 cacaggccgg ttaatttcgc gacccaatcc agacacgatg ctgcactcag cattacagac
     2461 attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt gtacagcgct ttctggcttg
     2521 cttaagaacc cttcgctgac agcaaaatag acccccgacg tccccagcga taacattgcc
     2581 tgattagcat caaccattcc cacaccaact gcattgtcgc caccgcctgc gacaactggc
     2641 accgtcgcca taccccacgc tttcgcaact tcaggtaaca aagcaccagt aatttcgctg
     2701 ccttcgtata atgcgggcat ctggtcacga gataagtcgc aagcctgcag catgacgtca
     2761 ctccagtcac gctttgcgac atccagccac atggtgccag ctgcgtcaga catatcgctg
     2821 gcaaactccc ccgtcatacg cagacgcaag taatctttcg gtaataatac tttgtcgatt
     2881 tgacggaata tctccggctc atgccgctga acccatagca atttaggcgc agtaaatccg
     2941 ggcatcatca ggttgccggt aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg
     3001 cactcttgcg cacagcgccc gtcgttccac aaaatggcag ggcgcaatac ccgttgttga
     3061 gcatccagta aggttgctcc atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc
     3121 agagaatgct gatcgcccag agctttcatt gcgcgatcag ttgcctgcca ccactgttcc
     3181 gggtcttgtt ccgaccagag tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa
     3241 gcaaccacct caccctgctc gttgagcaaa ataactttta cgcccgaggt gccaagatct
     3301 atcccgatat acatatcgat cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg
     3361 ggccaacgga ctgcacagtt agccgttatt tgtcgaacag ataatgattt accagatttt
     3421 ccagttgctc ctagcgacca ctctgatgca ccggagacaa attatgttcc tgagcatatt
     3481 tggctaaatc tgccagtgac atttggcctt tcaggatttg ctggcccaat tcgctattcc
     3541 agccggaata acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg
     3601 caattttcag cgccagtgcc atcgtatcca tcgcgccgat atgaccgtaa aacagatcat
     3661 atttatcagt actttgacga cgtactttgg catcgaagtt cagaccaccg gtggtgaaac
     3721 cgcctgcttt gagaatttca tacatcacca gcgcattctc ttccacactg ttcgggaact
     3781 ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc
     3841 caagcgcaat ggcggtggct atttcatgat ggaaagagtg acctgccagc gtcgcgtggt
     3901 tagcttcaat gttcagttta atctcttttt ccagaccaaa ctgtttcagg aagccataga
     3961 ccgtcgcggc atcgtaatca tattgatgtt tagtcggttc ttgcggtttc ggttcgataa
     4021 gcaacgtgcc ctggaagccg attttatgtt tatgctcaac caccatctgc ataaagcggc
     4081 ccagttgttc acgctcctga cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac
     4141 caccccacag gacatagttt tcaccgccca atttatgggt tgcttccatc gctgtaacaa
     4201 cttgcgttgc cgcccagctg aagacttcag gatctgggtt cgtcgccgca cccgcgccat
     4261 agcgagggtt tgtaaagcag ttagcggttc cccacagcag cttcacgccg ctctcttctt
     4321 gcttggctgc caggacatca accatttgcg caaaattatt gatgtactct tttaacgacg
     4381 cgccctcagg ggaaacatcc acatcgtgga agcaataaaa tgacacatgt aacttgtgga
     4441 aaaactcaaa tgcgacatct gctttacgct tcgccaacgc cagtgcctca ccaggctgct
     4501 gccacggacg attaaacgcc cccacaccaa acatgtccgc cccgttccag cagaaggtgt
     4561 gccagtaaca ggcggcaaaa cgcaagtgct cttccatacg cttacccaac accagttcgt
     4621 cgggattgta gtgacggaat gctaacgggt ttgaggattt tgagccttca taacgaacgc
     4681 gatcgagctg gtcaaaatag gcttgcatat tgaactccat aatcaggtaa tgccgcgggt
     4741 gatggatgat gtcgtaatat tgggcactcc ctttcagttg ctcaattatg ttatttcaca
     4801 ctgctattga gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg gatgatgaaa
     4861 ctgggtaatt cctcgaagat aaaaatgcaa taagtacaat tgcgcaacaa aagtaagatc
     4921 tcggtcataa atcaagaaat aaaccaaaaa tcgtaatcga aagataaaaa tctgtaattg
     4981 ttttcccctg tttagttgct aaaaattggt tacgtttatc gcggtgattg ttacttatta
     5041 aagctgtcct ctaactacag aaggccctac accatgaaaa taaagaacat tctactcacc
     5101 ctttgcacct cacttctgct taccaacgtt gcggcacacg ccaaagaaat caaaataggt
     5161 atggcgattg atgatctacg tcttgaacgc tggcaaaaag atcgagatat ttttgtgaaa
     5221 aaggctgaat ctctcggtgc gaaagtattt gtacagtctg caaatggcaa tggagaaaca
     5281 caaatgtcgc agattgaaaa catgattaac cggggtgtcg atgttcttgt cattattccg
     5341 tataacggcc aggtattaag taacgttgta aaagaagcca aacaagaagg tattaaagta
     5401 ttagcttacg accgtatgat taacgatgcg gatatcgatt tttatatttc tttcgataac
     5461 gaaaaagtcg gtgaactgca ggcaaaagcc ctggtcgata ttgttccgca aggtaattac
     5521 ttcctgatgg gcggctcgcc ggtagataac aacgccaagc tgttccgcgc cggacaaatg
     5581 aaagtgttaa aaccttacgt tgattccgga aaaattaaag tcgttggtga ccaatgggtt
     5641 gatggctggt taccggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt
     5701 gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca
     5761 gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc
     5821 ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac tgccgcagaa
     5881 attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact gaataatggc
     5941 ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa
     6001 gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc
     6061 gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg
     6121 ttatgcctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga
     6181 ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg
     6241 ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg
     6301 aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac
     6361 gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg
     6421 aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga
     6481 cgctacgctg tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg
     6541 ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac
     6601 aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg
     6661 ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc
     6721 acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggtcagc
     6781 acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg
     6841 ggcgagagtt aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac
     6901 gtattgaaca tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg
     6961 tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac
     7021 gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt
     7081 atattgatgg caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg
     7141 cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa
     7201 atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg
     7261 cagagcaaaa atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg
     7321 atcttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc gcccgctgtc
     7381 tgttactcaa cccgcgcatt ctcattcttg atgaacctac caggggtatt gatattggcg
     7441 cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg
     7501 tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg
     7561 aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag
     7621 ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta
     7681 ccgacatccg gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt
     7741 gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc
     7801 gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga
     7861 atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta
     7921 ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc
     7981 attgtgacgt tggttctggg actgcttctc ggtgcgtgga acggatggtg ggtcgcgtac
     8041 cgtaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc
     8101 attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc acaaattggg
     8161 caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt aatggctttt
     8221 gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca gtctccggcc
     8281 tctaccgcag tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg
     8341 ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc
     8401 ggcggaatgt ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc
     8461 aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc
     8521 gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct
     8581 ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcta ttgcagcatg cgtgattggc
     8641 ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc
     8701 atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc
     8761 gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct
     8821 tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat
     8881 gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg
     8941 ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac
     9001 aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg
     9061 actggttagg agatggcgtc attgccgact tcgacgacaa acaaatcgag caagcgctgg
     9121 ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc
     9181 cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt
     9241 taaaagagaa aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac
     9301 gttgggccac tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg
     9361 gagtggttta tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc
     9421 tggcagactg gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc
     9481 gggcgcggca tattctgcaa gtatgtgaac atctacacat tcccgtaccg gaaaaattat
     9541 gcgtgattgg catcgacaac gaagaactga cccgctatct gtcgcgtgtc gccctttctt
     9601 cggtcgctca gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat
     9661 tagataaaga agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac
     9721 ggcgctcaac agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca
     9781 ttcgtaatca cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct
     9841 cgcgctccaa tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga
     9901 ttcatgccga gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca
     9961 atgagatatc gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa
    10021 aagcatatga cacaacgcca aaagagtatc gcgatgtaaa tcgcgaggtc atgttgtagc
    10081 gatgatgaga attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa
    10141 aagccttatc cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg
    10201 cgattaaccg ttggttatcc tggtacattg cgaacaggta gttattataa ctcttccctt
    10261 tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt
    10321 tacgcagctg cgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag
    10381 tgacataggc gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac
    10441 ccggcgcatt ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc
    10501 gcgccagctt cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg
    10561 tagggataat gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag
    10621 accacttcac cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc
    10681 attggccctg atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt
    10741 ggctggtaat gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag
    10801 ggtattttcg caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt
    10861 tactgcttac ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg
    10921 tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg
    10981 ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc
    11041 aacaccttat atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg
    11101 tcaagagcac cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga
    11161 ttggcgctaa atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcgc
    11221 gatcgctcac ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg
    11281 cttatcgctg gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg
    11341 cctcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg
    11401 ggttccctgc ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca
    11461 aaggtacgcg tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga
    11521 taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat
    11581 tgttcaggga cggcaaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga
    11641 tgatttccat ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa tgcccgaaat
    11701 gggatggatt accgctcacg ctggatgtca gcgccacttt cccggaagga gccgccgtac
    11761 gggattatta cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg
    11821 ctgctaccag caacggttta ctcctgctgg aacgggcaga aactgacaca tccgcccctt
    11881 tcgactggca taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc
    11941 ccagtaatga ccagagttac ggacgtcata aagacggtat ggcggaaatt ggcacttttc
    12001 acggcggcga tttacgcggc ctgatcaaca aactggatta cctccagcag ttgggcgtta
    12061 atgctttatg gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa
    12121 aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg
    12181 atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg
    12241 gtattcgtat tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata
    12301 tgcaggagta tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaaacgctgg
    12361 gtgaacgctg gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt
    12421 acattaattt cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa
    12481 cggatatcgg cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt
    12541 tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca
    12601 aaacggatac tcacgctaaa gtcatcgaag gctttacacc tcgcgattac ttaacccact
    12661 ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac
    12721 atgttgagtt gcccgcctgg cagcaactga aaaccgaagc cagctccgcg cttcgcgaat
    12781 ggaaaaaaac taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag
    12841 cctggggcca cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca
    12901 atttcgatta tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga
    12961 cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc
    13021 atgatacccg tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc
    13081 caggcgcggt acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt
    13141 ctgatccgct gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg
    13201 ccgccaacgt cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg
    13261 gcgcgggcaa acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg
    13321 gcgacgataa agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc
    13381 atcagtacct cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg
    13441 ataaagcacc gctcactctt caacagaccg atttgcaccc cagcaaatgt agcgttattg
    13501 ttaccttcct tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg
    13561 acaaatttac ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac
    13621 gcacgcctgg cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg
    13681 actacttcca gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt
    13741 gtaactacga cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc
    13801 gcgagaagtt gggttgggat atcgaaccac agaatattgc actaacaaac ggcagccaga
    13861 gcgcgttttt ctacttattt aacctgtttg ccggacgccg tgccgatggt cgggtcaaaa
    13921 aagtgctgtt cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag
    13981 atctgtttgt ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc
    14041 acgtcgattt tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc
    14101 cgacgaatcc aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg
    14161 cgaatcaaca cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta
    14221 tcatcttcag tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt
    14281 ccaagctggg gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca
    14341 ctgccatcac caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga
    14401 tgatgtgtga aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac
    14461 cgttttacta ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaaa
    14521 atcgctgcct gattcataaa ccggaaggag ccattttcct ctggctatgg tttaaggatt
    14581 tgcccattac gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc
    14641 cggggcacaa cgtcttcccg ggactggata aaccgtggcc gcatacgcat caatgtatgc
    14701 gcatgaacta cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag
    14761 agatagaaag agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag
    14821 gcctacatga tttctgcaat atattgaatt tggaagaatt tgtaggccgg ataaggcgtt
    14881 tacgccgcat ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaacg
    14941 tttcaaacag gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc
    15001 aacgcctttg tcgggcaggc ttcaacacag gccggccccg tctcccgatg ccagcacaga
    15061 tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg
    15121 caagccagca tacagctttt acagccaatg caacgtgttt gttcaacgaa aatatgccca
    15181 tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc acggtgcatc ttcacactga
    15241 tgacaggcta ctgccgtggt ccagctgtgg tctttaatga cacgaatacg ggaaataaac
    15301 tcgtctggtg acaacgcagc gcaatcctga ttctcctgat gcgacactgc gcaagccact
    15361 tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc
    15421 actcctttgc actaaaacag cagagtgcca gagagcgcgt ccctactgct ttgatccaac
    15481 gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg cccaggtcat
    15541 ccctgacagt aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc agtggtttca
    15601 ggagattttt ctctcccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt
    15661 acggcacccg cccatgaata tcaaacaccg gaacagcaat acaggagacg ccgagttcgt
    15721 tttcttctct gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg tcgaacatcg
    15781 cgggcagctc ggtaatggta ttgcgggtta acggctggat ctcatgctga tggttttccc
    15841 agtatgactt cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac
    15901 agtagagcgg catatgctgg ccaatatagg cacgggttcg cagcatcccg gttgtgggtt
    15961 ccagcttata aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atggtttcac
    16021 cagtggcgat gttcagtgcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag
    16081 acagcgcctt ctgcccgacg gcaataaatt tggtagtcag gcggtaactc cctgcggcgg
    16141 gcgcggtggt cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct
    16201 tatttaaacc agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca
    16261 aaatctcaat cagcatcaac ccgcgaaaca gactctggct tccggctgga cgctcttttt
    16321 cctgcgccat ctcgttctct ttttttccca tcacttcttt ccccattttg tcgcgtcctg
    16381 atggtagcgc aaagtgtgcc gtagttcacg atctcgacag ataatttata accacttgat
    16441 ttttatgtct tttgaaattc atcaatcaga ttgctttgtt aaaaagtgat cgatatattt
    16501 gaaatcaagt ttcgcatatt gaaattttaa gccaaaaaag cgatcaaaaa aacaaggaag
    16561 cctgggatga aagtgacatt tgagcagtta aaagcagcct ttaatcgggc cttaatttca
    16621 cgcggcgttg acagcgaaac ggctgacgcc tgtgcagaga tgttcgcccg caccaccgaa
    16681 tccggcgttt attctcacgg cgttaatcgt ttccctcgtt tcattcaaca actggaaaac
    16741 ggcgatatca ttcctgatgc ccaacccaaa cgtataacca gcctcggcgc aattgaacag
    16801 tgggatgccc agcgttcgat cggcaacctg acggcgaaaa agatgatgga tcgcgccatt
    16861 gaactggctg ccgatcacgg tattggtctg gtggcacttc gtaatgccaa ccactggatg
    16921 cgcggcggca gctacggctg gcaggcggcg gaaaaaggct atattggcat ttgctggacc
    16981 aactccatcg ccgtaatgcc gccgtggggc gcaaaagagt gtcgcattgg cactaacccg
    17041 ctgatcgtcg ccattccttc cacgccgatc accatggtcg atatgtcgat gtcgatgttc
    17101 tcttacggca tgttagaagt taaccgcctg gcgggccgtc agctcccggt cgatggtggc
    17161 tttgatgatg agggcaattt gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt
    17221 ttgccgatgg gctactggaa aggttctggc atgtcgattg tgctggatat gatcgctact
    17281 ctcctttccg acggcgcatc ggttgccgaa gtcacccagg acaacagcga cgaatacggc
    17341 atttcacaaa tttttattgc cattgaagtg gacaagctta tcgacggtcc cacccgcgat
    17401 gccaagctgc aacgcatcat ggattacgtt actaccgctg aacgcgctga cgaaaaccag
    17461 gccatccgct tacccggcca cgaatttact accctgctgg ccgaaaaccg ccgtaacggc
    17521 atcactgttg atgacagcgt gtgggccaaa atccaggcgt tatgaggaga taagtcatga
    17581 tttttggaca tatcgcgcag cccaatccgt gccgtttgcc cgccgccatt gaaaaggcgc
    17641 tcgattttct gcgcgctacc aacttcaacg tcctggagcc gggcgttgtc gaaatcgacg
    17701 gcaagaatat ttatgcgcaa attatcgatt taaccacacg cgaagcggtg gaaaaccgtc
    17761 cggaagtgca tcgtcggtat atcgatattc agtttctggc gtggggtcaa gagaaaatcg
    17821 gcattgctat tgatacagga aataataaag tcagcgaatc actattagag cagcgcgata
    17881 ttatttttta tcatgtcagt gaacatgaat catttataga aatgataccg ggcagctacg
    17941 ctattttctt cccgcaggat gttcaccgac caggttgtat tctgcaaaca ggcctctgag
    18001 atacgaaaaa ttgtggttaa agtcgcatta acagcgctga attaatcaaa taatcgtcta
    18061 cgccagaacg actgggtaat gttattgctc taactatatt aataatttag gttgctgcac
    18121 atgcaacttg aattatgaca gacatagcct caaggaatag ctatgaaaaa aatacttgaa
    18181 gtaatactgg cgattaatct cgccgtactt tcctgtattg tgtttataaa cattatttta
    18241 agatatggtt ttcagacaag cattttatct gtagatgaat tgtcacgtta tttatttgtc
    18301 tggttaacat ttattggcgc gattgtggct tttatggata acgcccacgt tcaggtcacc
    18361 tttctggtgg agaaactctc ccccgcatgg caacgacgag tcgctctggt tacccattcc
    18421 ttaatcttat ttatttgtgg cgcactggcc tggggcgcaa cactaaaaac aattcaggac
    18481 tggagcgatt attcaccgat cctcggtttg cccatcggcc tgatgtatgc cgcttgtctg
    18541 cccaccagcc tcgttatcgc attctttgaa ctgcgtcatt tatatcaact catcacgcgt
    18601 agcaattctc taacgtcgcc accgcaagga gcttaatcat ggctgtgctg atttttctgg
    18661 gctgtctgtt gggtggtatt gctatcggtt tgcctattgc ctgggcactg ttgttgtgcg
    18721 gggcggcgtt aatgttctgg ctggacatgt ttgatgtcca gatcatggca caaacgctgg
    18781 tgaacggagc cgatagcttc tccctgctgg cgattccgtt ctttgtgctg gcgggtgaaa
    18841 tcatgaatgc gggcggcttg tcaaaacgca ttgttgacct gccgatgaaa ctggtggggc
    18901 ataaaccagg cgggctgggc tacgtcggcg tgctggcagc tatgattatg gcgagccttt
    18961 ctggttctgc cgttgcggat accgccgccg ttgccgctct actggtgccg atgatgcgca
    19021 gcgccaacta cccggtcaac cgggcggcgg ggctgattgc ctctggcggc attatcgcgc
    19081 caattattcc cccttccatt ccgtttatta tcttcggcgt ttccagcgga ttatccatca
    19141 gtaagctgtt tatggcgggc attgcccctg acatgatgat gggcgcaacg ctgatgctta
    19201 cctggtggtg gcaggccagt cgccttaatt taccccgcca gcaaaaagca acgatgcagg
    19261 aaatctggca ctcttttgtc tccggtatct gggctctgtt tcttccagtc atcattattg
    19321 gcgggtttcg ctccggtctg tttaccccta ccgaagccgg ggccgttgct gctttctatg
    19381 cgctgtttgt cgccacagtt atttaccgtg aaatgacctt cgccacactt tggcatgtac
    19441 tcattggcgc ggcgaaaacc acctcagtgg ttatgtttct ggtggcctcg gcacaa
//
LOCUS       DAJVTY010000070        19457 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVTY010000070 DAJVTY010000000
VERSION     DAJVTY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.070"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..422
                     /gene="eutC"
                     /locus_tag="OQQ66_002187"
     CDS             <1..422
                     /gene="eutC"
                     /locus_tag="OQQ66_002187"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCS1905107.1"
                     /translation="ANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPF
                     FVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTC
                     ISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            432..1091
                     /gene="eutL"
                     /locus_tag="OQQ66_002188"
     CDS             432..1091
                     /gene="eutL"
                     /locus_tag="OQQ66_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1905108.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1104..1604
                     /gene="eutK"
                     /locus_tag="OQQ66_002189"
     CDS             1104..1604
                     /gene="eutK"
                     /locus_tag="OQQ66_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1905109.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKESGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            1650..2702
                     /gene="eutR"
                     /locus_tag="OQQ66_002190"
     CDS             1650..2702
                     /gene="eutR"
                     /locus_tag="OQQ66_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS1905110.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(2708..3607)
                     /gene="hemF"
                     /locus_tag="OQQ66_002191"
     CDS             complement(2708..3607)
                     /gene="hemF"
                     /locus_tag="OQQ66_002191"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1905111.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(3611..4480)
                     /gene="amiA"
                     /locus_tag="OQQ66_002192"
     CDS             complement(3611..4480)
                     /gene="amiA"
                     /locus_tag="OQQ66_002192"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1905112.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            4694..5119
                     /locus_tag="OQQ66_002193"
     CDS             4694..5119
                     /locus_tag="OQQ66_002193"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1905113.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5106..5555
                     /locus_tag="OQQ66_002194"
     CDS             5106..5555
                     /locus_tag="OQQ66_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1905114.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            5762..6337
                     /locus_tag="OQQ66_002195"
     CDS             5762..6337
                     /locus_tag="OQQ66_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1905115.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6433..7332
                     /gene="yfeX"
                     /locus_tag="OQQ66_002196"
     CDS             6433..7332
                     /gene="yfeX"
                     /locus_tag="OQQ66_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1905116.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7390..8694)
                     /gene="pbp4b"
                     /locus_tag="OQQ66_002197"
                     /pseudo
     CDS             complement(7390..8694)
                     /gene="pbp4b"
                     /locus_tag="OQQ66_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(8699..10123)
                     /gene="murP"
                     /locus_tag="OQQ66_002198"
     CDS             complement(8699..10123)
                     /gene="murP"
                     /locus_tag="OQQ66_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1905117.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10127..11023)
                     /gene="murQ"
                     /locus_tag="OQQ66_002199"
     CDS             complement(10127..11023)
                     /gene="murQ"
                     /locus_tag="OQQ66_002199"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1905118.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11187..12044
                     /gene="murR"
                     /locus_tag="OQQ66_002200"
     CDS             11187..12044
                     /gene="murR"
                     /locus_tag="OQQ66_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1905119.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12173..12964
                     /gene="ucpA"
                     /locus_tag="OQQ66_002201"
     CDS             12173..12964
                     /gene="ucpA"
                     /locus_tag="OQQ66_002201"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1905120.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13268..14284
                     /gene="cysP"
                     /locus_tag="OQQ66_002202"
     CDS             13268..14284
                     /gene="cysP"
                     /locus_tag="OQQ66_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1905121.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14284..15117
                     /gene="cysT"
                     /locus_tag="OQQ66_002203"
     CDS             14284..15117
                     /gene="cysT"
                     /locus_tag="OQQ66_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1905122.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15117..15992
                     /gene="cysW"
                     /locus_tag="OQQ66_002204"
     CDS             15117..15992
                     /gene="cysW"
                     /locus_tag="OQQ66_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1905123.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            15982..17079
                     /gene="cysA"
                     /locus_tag="OQQ66_002205"
     CDS             15982..17079
                     /gene="cysA"
                     /locus_tag="OQQ66_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1905124.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17213..18124
                     /gene="cysM"
                     /locus_tag="OQQ66_002206"
     CDS             17213..18124
                     /gene="cysM"
                     /locus_tag="OQQ66_002206"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1905125.1"
                     /translation="MSTLEQTIGNTPLIKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18127..18495)
                     /locus_tag="OQQ66_002207"
     CDS             complement(18127..18495)
                     /locus_tag="OQQ66_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1905126.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            18600..19451
                     /gene="pdxK"
                     /locus_tag="OQQ66_002208"
                     /gene_synonym="thiJ"
     CDS             18600..19451
                     /gene="pdxK"
                     /locus_tag="OQQ66_002208"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1905127.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4484 a         4925 c         5479 g         4569 t
ORIGIN      
        1 ttgctaatcc ggacgtacag gtcgttattt ctgatggcct gtcaactgat gccatcaccg
       61 tgaactacga agagatcctg ccgccgctga tggcgggcct gaaacaggcc gggctgaaag
      121 tcggcacgcc attctttgtt cgttatggtc gcgtgaagat tgaagatcag atcggtgaga
      181 tcctcggcgc gaaagtggtg atcctgctgg tgggggaacg tccggggttg ggtcagtcag
      241 aaagcctctc ctgttacgcc gtttactcgc cgcgtatggc gaccaccgtt gaggccgatc
      301 gcacctgcat ctctaacatt caccagggcg gcacgccgcc ggttgaagcc gcagccgtca
      361 ttgtggattt ggcgaaacgt atgctggagc agaaagcatc cggcatcaac atgacccgat
      421 aaggaggcat catgccagct ttagatttga ttcgaccgtc ggtaaccgcc atgcgggtga
      481 ttgcctccgt taacgctgaa tttgcgcgtg aactaaaatt gccgccgcat attcgtagcc
      541 tcgggctgat ttctgctgat tccgatgacg tcacctacat tgcggctgac gaagcgacca
      601 agcaggcgat ggtcgaagtg gtatatggcc gctcgctgta tgcgggagca gcacacggac
      661 cgtcaccgac tgccggtgaa gtgctgatta tgcttggtgg gccaaacccg gcggaagtgc
      721 gtgctggtct ggatgcgatg gttgcgcata ttgaaaatgg cgctgctttc cagtgggcta
      781 atgacgcaga aaacaccgca ttcctggcac atgtagtttc gcgtaccggt tcttatctct
      841 catcaaccgc cgggatcacg cttggcgatc cgatggcgta tctggtggca ccgccgctgg
      901 aagcgaccta cggcattgat gcagcgttga aatctgccga cgtacagctg gtgacctatg
      961 tcccgccacc gtctgaaacc aactactcgg cagcattttt aaccggtagc caggccgcgt
     1021 gtaaagcagc ctgtaacgcc tttaccgatg cagtgctgga aatcgcgcgt aatccaatcc
     1081 agcgtgcgta acggaggttg ccgatgatca atgcactggg attgctggaa gtggacggca
     1141 tggtcgccgc gatagatgca gcggatgcca tgctcaaagc agctaacgtt cgtctgctca
     1201 gtcacgaagt gcttgaccct ggtcgcttaa cgctggtggt ggaaggcgat ctggcggcgg
     1261 gtcgtgcggc gctggacgca ggttgtgctg ccgcgatgcg taccgggcgt gtcatcagcc
     1321 gcaaggagag cggtcggcca gacgatgaca cccagtggct ggttactggc tttaaccgcc
     1381 agccgaagca acccgtaagg gaacccgacg cgccagttat cgtcgcggaa tctgctgacg
     1441 agttgttggc gctgttaaca tcagtacgtc agggaatgac ggcaggagaa gtggctgccc
     1501 actttggctg gccgctggaa aaagccagaa atgcgctcga acagctcttt tctgccggga
     1561 cgttacgtaa acgcagtagt cgttatcgtc tcaagcccca ttaacctgtc ggaggtgctg
     1621 ggtgtcctac aacacccggc attaacatca tgaaaaagac ccgtacagcc aatttgcacc
     1681 atctttatca tgaaccctta cccgaaaacc tgaagctcac gccgaaggtc gaagtggata
     1741 atgttcatca acgacagaca acggatgtct atgaacatgc tttaacgatt accgcctggc
     1801 agcagattta cgatcagctg catccgggca agtttcatgg tgaatttacg gaaattctac
     1861 tcgatgatat tcaggttttt cgtgaataca ccggtctggc gctgcgtcag tcgtgcctgg
     1921 tctggccgaa ctcgttctgg tttggcattc cggcgacgcg cggtgagcag ggatttatcg
     1981 gttcgcaatg tctgggaagc gcggaaatcg ccacccgccc tggtggcact gaatttgaac
     2041 tgagcacgcc ggatgattac acgatcctgg gcgtggtgct ttctgaagat gtcatcaccc
     2101 ggcaggctaa ctttttgcat aacccggatc gggtattaca tatgctgcgt agccagtcgg
     2161 cgctggaagt gaaagagcag cataaagccg cgctgtgggg ctttgtccaa caggcgctgg
     2221 cgacgttttg cgagaacccg gaaaatctcc atcagcctgc agtgcgaaaa gtgctggggg
     2281 ataatttgct aatggcgatg ggggcgatgc tggaagacgc gcaaccaatg gtgacggcgg
     2341 aaagcatcag tcatcagagt taccgtagat tactttcccg cgcccgtgaa tatgtgctgg
     2401 aaaatatgtc tgagccggtg acggtgctgg acttgtgtaa tcaactgcat gtcagtcgcc
     2461 gcacgctaca aaacgcgttt cacgctattt taggcattgg cccaaacgcg tggctgaaac
     2521 gcattcgcct gaacgccgta cgccgcgaac tgataagccc gtggtcgcaa agcacaacgg
     2581 taaaagacgc cgccatgcag tggggattct ggcatctggg gcaatttgtc acggattacc
     2641 agcagctgtt tgccgagaag ccgtcgttga cgttgcatca gcggatgcgg gaatgggggt
     2701 gagggagtta cacccaatcc ctgaccttaa taaactcact taacgccgct tctgggctgc
     2761 catcttttgg ctgataatca tactcccagc gtaccagtgg cggcattgac atcaggatcg
     2821 actcggtgcg cccgccagtt tgcaggccaa acagagtgcc gcgatcccag accagattga
     2881 actcgacata acgaccgcga cggtagagct gaaaattgcg ctcgcgctcg ccgtaggcca
     2941 ttgctttacg tcgctctaca attggtaaat aagcgtcggt gtagcctttg cctaccgcct
     3001 gcataaaggc aaaacagtgg tcgaaatctg gcgtgttcag atcatcaaag aacagcccgc
     3061 caataccgcg ctgttcgttg cgatgtttga ggtagaagta atcgtcgcac cactttttgt
     3121 aacggggata aacgtcttca ccaaatggca ggcacaggtc acgggcggtg cggtgccagt
     3181 gaatggcgtc ttcttcaaaa ccatagaaag gggttaaatc gaagccgccg ccaaaccacc
     3241 agacgggctc tgcacccggt ttttcggcaa taaaaaaacg cacattcgcg tggctggtgg
     3301 gaacatacgg gttatgcgga tgcactacca gtgaaacgcc catcgcctcg aaactgcgcc
     3361 cggcaagttc cgggcgatga gcggtggcgg aagcaggcat cgcctcgccg tggacgtgag
     3421 aaaagttgac gcctgcctgt tcgaaaacac caccattacg caacacccga ctacgcccgc
     3481 cgccgccagc ttcgcgctgc caactatctt cgacaaattc tgcgccatcg acggcggtca
     3541 gctgctgaca aatcgtatcc tgaaggttga gcagaaactg tttaacctgg tgtgcgtcgg
     3601 gtttcataac ttatcgcttt ttcgaatgtg ctttctggtt gtcgaaccag tggaaataac
     3661 tgatcacgcc ttcagcaatc gctgtggcga ttttctgacg aaacgccgcc gtgcctaaca
     3721 gccgctcttc ttccgggttg gtgataaacg aggtttccac cagcaccgaa ggaaccgacg
     3781 gcgatttcaa caccacaaac gcggcttgtt cggtgttgcg gctgtgcagt ttatgcaccg
     3841 gcttaatctt tttcagaata tgcgagccaa gcgtcaggct gtttttaata gtatcggttt
     3901 gcaccagatc aaacagcact tgctgcaata ggtgatcctt gtcagtcgcc tttttaccgg
     3961 caacttcatc ggcgcggttt tcgcgttcag acaggtattt cgccattgcg ctacttgccc
     4021 cacggttaga gagggcaaat accgaagcac cggcagcttt cgggttggta aagccatcgg
     4081 catgaattga cataaacaga tctgcgccat gtttatgggc gatttcaacg cgatcgtaaa
     4141 gtgggataaa cgtatcgcca gaacgcgtta aacgcgcatc aatcccatga ttacgcaaaa
     4201 tggaacggac gtttttagca atcgccagca ccacatgttt ttctttcgaa ccgttgcgtc
     4261 cgatcgctcc ggtatcaatt ccgccgtggc ctggatcgag aacaacgaca cgtttgccgc
     4321 cagatttttt ggctttcggc ttgctgtgtc cgttgctggt ttttaaaggt tcgtctttgg
     4381 cgatggcttg cgacattcct gacaacgtca gggcagccaa accggctttc agcacctggc
     4441 ggcgcgaagt gagtgttttt agtggtttaa aagtgctcat acggcctgag ttgtaataat
     4501 aaagttccag atgttatatc gtatcgcgta atccgttacg acggtttgat gttgagaatt
     4561 gttttacttt tcatttcaat agttgacaac tccccattat gccacttttt caaccgattt
     4621 tcgtcagata ttggctaatt ctaccgttca cgccataatc agccttttaa cccgcaaaaa
     4681 ggtaacgaat accatggaga tacgcgtatt tcgccaggaa gatttcgaag aggtcatcac
     4741 cctttgggag cgttgcgact tgctgcgtcc gtggaacgat ccggaaatgg acatcgagcg
     4801 taagatgaac catgacgtca gtttgttttt ggtcgctgag gtgaacggtg aagtggtcgg
     4861 aacggtgatg ggcggttatg acgggcatcg cgggtctgct tattatcttg gcgtgcatcc
     4921 agagtttcgt gggcgtggga ttgccaatgc gttgcttaat cggctggaga aaaagctgat
     4981 tgctcgtggc tgcccgaaaa ttcagatcaa tgtgccggaa gataacgaca tggtgctcgg
     5041 aatgtatgaa cgcctgggat atgaacacgc cgacatgctg agtctgggta agcgtttgat
     5101 tgaagatgaa gagtactgag ttccatcctg tccattatga tgcgcacgga cgcctgcgtt
     5161 tacccttgct cttctggctt gtgctattgc ttcaggcgcg aacctgggtg ctgtttatca
     5221 ttgccggtgc gtcgcgcgag cagggtactg cgctgctaaa tctgttttat cccgatcacg
     5281 ataatttctg gctggggttg attcccggta ttccggcggt gctggcgttt ttgttgagcg
     5341 gtcggcgggc ttcgtttcct cgcacctggc atgtgctcta ttttctgttg ctgttggcgc
     5401 aggtggtttt actttgctgg caaccgtggc tgtggctgaa cggtgaatcc gttagcggta
     5461 tcggtctggc gctggtagtg gcggatattg tggcgttaat ctggctgctg accaatcgac
     5521 gtttacgcgc ttgttttaat gaagaaaaag aataacgcag aggtgcgctt tgtttatgcc
     5581 ggatgcggcg tgaacgcctt atccggccta caaaacatcg aaaattcaat atattgcagg
     5641 agaaagtagg cctgataagc gtagcgcatc aggcaatttt gtgtttgtca tcaaccttta
     5701 acggcacttt ttggtgaatt tgcactccaa gcaacgttat tgaataacca aaggaagtga
     5761 catgaaatcg ctgcgtttaa tgttatgcgc tatgccgttg atgctgaccg gctgttccac
     5821 gatgtcgtca gttaactggt cggctgctaa cccgtggaac tggtttggat cgtccaccaa
     5881 agtgagcgag cagggtgtgg gtgaattaac ggcgtccaca ccactgcaag aacaagccat
     5941 tgctgatgcg cttgatggcg attatcgcct gcgcagcgga atgaaaaccg cgaacggcaa
     6001 cgtggtgcgc ttttttgaag tgatgaaagg cgacaacgtg gcgatggtga ttaacggcga
     6061 tcaaggcacg atcagtcgca ttgacgtgct ggatagcgat attcctgctg acaccggtgt
     6121 taaaatcggt acaccgttta gcgaccttta cagcaaagca tttggcaatt gccaaaaagc
     6181 tgatggtgat gataatcgtg ccgtcgaatg taaagccgaa ggcagtcaac atattagcta
     6241 ccagttcaga ggggaatggc gtggtcctga agggttaatg ccttcggacg ataccctgaa
     6301 aaactggaaa gtcagtaaaa ttatctggcg gcgttaattt gcgtctgaac aaaccgccgc
     6361 cgcgaaaaaa cacgtaaaat agcgctcaac aatgccacgg attgcgtggc attcttattt
     6421 tcaggaggaa caatgtctca ggttcagagt ggcattttgc cagaacattg ccgcgcggcg
     6481 atttggatcg aagccaacgt gaaaggggaa gttgacgccc tgcgtgcggc cagtaaaaca
     6541 tttgccgaca aactggcaac ttttgaagcg aaattcccgg acgcgcatct tggtgcggtg
     6601 gttgcctttg gtaacaacac ctggcgcgct ctgagcggcg gcgttggggc agaagagctg
     6661 aaagattttc cgggctacgg taaaggcctt gcgccgacga cccagttcga tgtgttgatc
     6721 cacattcttt ctctgcgtca cgacgtaaac ttctctgtcg ctcaggcggc gatggaagcc
     6781 tttggtgact gcattgaagt gaaagaagag atccacggct tccgttgggt ggaagagcgt
     6841 gacctgagcg gctttgttga cggtacagaa aacccggcgg gtgaagagac gcgccgcgaa
     6901 gtggcggtta tcaaagacgg cgtggatgcc ggcggcagct atgtgtttgt gcagcgttgg
     6961 gagcacaacc tgaagcagct caaccggatg agcgttcacg atcaggagat gatgatcggg
     7021 cgcaccaaag aggccaacga agagattgac ggcgacgaac gtccggaaac ctctcacctc
     7081 acccgcgttg atctgaaaga agatggcaaa gggctgaaga ttgttcgcca gagcctgccg
     7141 tacggcaccg ccagtggcac tcacggtctg tacttctgcg cctactgcgc gcgtctgcat
     7201 aacattgagc agcaactgct gagcatgttt ggcgataccg atggtaagcg tgatgcgatg
     7261 ttgcgcttca ccaaaccggt aaccggcggc tattacttcg cgccgtcgct ggacaagctg
     7321 atggcgctgt aagcgtttct gattctatga ttaaaggcca gccagtaaca aaactgactg
     7381 gcctttttat tacttttgct tcaacgccgc atacacctga tcaactaccc aaccataagt
     7441 tgcaatcggt aactgaccgc tttcgaacat attgggattt ttttgtggat cggcaactgg
     7501 cgaatggggc ttgttgctta acatcacaat cgccatatga ttgaccggat cgataacggt
     7561 tactgtcccg gtccagccag tgtgaccgta ggtttgcggg cttgccagcg tgccaaacgt
     7621 cggcgtcatg gtggcattac cattcacgcg ccagccgagg ccaaaagtgg catcttcctt
     7681 agagctggtg gtgaacatct tcaccgtttc cgcactgaac agctgcacat cgccatagcc
     7741 cccgccgttc agcatcgttt gcattaacac cgcaatatcg ccggtattgg aaaacaaacc
     7801 tgcgtgcccg gaaacgccgc ccatcgaata aaaggctttt tcatcgtgca cctgacccca
     7861 gagagtggag gtgcggatat tcgggaaatg gatcacgcca tcgcgggtat tgccgttcaa
     7921 ttccgtggcg gcaatttgtt gcggtttaaa gcctttcagc agcgggttaa ataccgtatg
     7981 tgtcaggccg agcgggcgat aaatcgactc ttcaacatag cggtcaagcg gctgaccggt
     8041 aaccgactcg acgataaatc caagcagcat ataatcgaca tcgctgtaga tatgtttgct
     8101 gccgggctga tattccagcg gcgtgcgctt gatcatctcc agcgtctggc ctttatccta
     8161 ggaatataac gcgcccgcga ccgctttatt cgggtattgc ggatctgccg ggaaaccgcc
     8221 gctgtgatgc agcaggtcag aaatccgcag ggtgtttttg cctttaatgg tgtcatttgg
     8281 actatcggca aaccccggaa tatatttcgc aatccgatcg tcaggatgca gtttgccttc
     8341 gctcatcagc ttttgcaggg cgaagttcgt ggcgtacatt ttggtgtttg aggccaggtc
     8401 atacagcgtc ccggtggtgg ctttgacagg ctgttccatc aacacgctgc catcgtactt
     8461 tttcgccgct ccccaggctt tgcgatacac aatctgatta tctttaatga tcagcaggtt
     8521 tacgccggga taaccggcat caacttgctg gctaatccag cgatccatct ggttaagccg
     8581 tttgacgtta aaccctgctt tctctggcga gctttctgtc agaacagggt attttgcggc
     8641 gctgacgcta caactaacag ccagcagaga aagatagagc attgtccgtt tcatgatatc
     8701 agtccagatt gacgttacgg cagccaaaga gcgtggtgaa aataaacccg caaacccatg
     8761 ccaccagaat cccgccagca taaaccgcca tggccggaag gatgccttgt gcggaagtca
     8821 tcagcggcag cgctaccaga ccagacggcc caaaggcgct gtttaagccc atcggcagac
     8881 cccaccaggc tatcaggccg ataaacaaac cgcccgccgc accgcctaaa caggcggtga
     8941 caaacggctt catgcggggc agggtgacac cgtaaatcag cggttcacca acgcccagca
     9001 ggccgggaat aatcgccccg cgtacctgac tgcgtagcgc actgtgcggt tgcgaccgcc
     9061 agtagagtgc cagcgccgcg cccacctggc ccgcgcctgc cattgaaagg atgggaaata
     9121 agctgttgaa tccctggctg tccattaacg cgaggtaaac aggaataaag ccctgatgca
     9181 cgccaaatac cacggcgatc aggaacagcc cggctaaaac cgcacaaccg aacggattac
     9241 tgttcaggtg cataaacagc catgacatac cttcgaataa ccagccaccc agcggcataa
     9301 tgatcaggta cgcaagcgta gcggtgatca gcagggtgat taacgaggtc agcagcatgt
     9361 cgagatcgtc cggcataaag cggcgcacca tgccttcaat gcgggcgcag gcccacgcgg
     9421 caatcagcac gccgataata ttgccgcgcg gatcgatggg cagaccaaag aaatcgtgaa
     9481 aaccggcgta gtaaccggtg gttgcagcgg ggttataacc gagcaaaaag agtgcggcaa
     9541 taattgcgcc attgacgccc gtgccgccaa atgcctgggc ggcgttatag cccaccagaa
     9601 tcaccaggaa ggtgaacaaa cctttgctga acaccttcat aaaattcagc gcgtcgggta
     9661 gcgtgccctg agcatctgcc ggaacgtgca tcaccgtggc aattaacgtc gctatcccca
     9721 gcagcagacc ggcggcaata aaaccgggga tcagcggcgt gaagatggtg gcgaatttgg
     9781 cgagaaattg ttgcacgccg gaggtttgtt tggcttttaa ctgacgtttg ttctgggcgg
     9841 cgatttctgc ggcatcctgt accggtgcct cgcccagcag ctcgctcatc gcttttgcgg
     9901 cacgatgcgc tttaccaggt ccaaaaacga cctgcacctg gtcgctggtc aaaatgacgc
     9961 tcttcacgcc ttccagcgtt ttgatattgg gatcaaccag tgaactgtca tgtacaccca
    10021 gacgcaggcg cgtcatacag ttaccacaac tggcgatatt tcccggtccg ccgacacggg
    10081 taagaatggt gttcagaagt tcactgctga tctctttggc catgggttat tccttgtcta
    10141 aaacctgacg aataaagccg ccgtgttgat ccaggcgttt tttagcttct gcggcatcga
    10201 gatttttcag caccatcaca atggccgttt tacagttgcg ctcgcaggca attaacgccg
    10261 cttccgcttg ttcggcatta catccggtgg cgtttttaac gatattgacc tggcgcacat
    10321 gcagtttttc gttggtggcg accacatcga ccataaggtt gccgaacact ttgccggatt
    10381 taatcatcag cccggtggaa agcatattga gcaccagttt ctgcgctgta cctgctttca
    10441 tccgcgaaga accggtaaca acttcggcac ctacaatcgg tgtaatggca aactcagcgg
    10501 tggttgaaac ggcgctcccc ggattacagg aaattcccac tgtgcggcag ccgagctggc
    10561 gtgcgtattc cagtccggca atcacatacg gcgtgcgacc gctggcagca atgccaacca
    10621 ccacatcctg tgccgttaaa ttaatatttt tcagatcatt aacaccgcct tcccggctat
    10681 cttccgcgcc ttccaccgcg tgctgaatgg catattcgcc gccagcaatc aaaccaacca
    10741 ccagacccgg tttcacgccg taggtgggcg gacattcgct ggcatccaga atccccagac
    10801 gaccggatgt tcccgcaccg aggtaaatca gacgcccgcc gccgcttacc tgggcgtgga
    10861 taacatcgat cgccgcggcg atatccggca gtacgcgctc aacggcaagc ggtacggttt
    10921 tatcttcatc gttgataatc cggcacattt ccagcgttga tacgcggtca atttcagccg
    10981 aggcggcgtt cgagccttca gtaatcatct tttcaagttg catcggtggt gaccttacta
    11041 tttacgatca aataattgca gatgtatgga atcatatatc ctgaattgac agacttaaag
    11101 gaatcattga gtctacactc gcagagtgtg aagagcgtca taagaactta agcggaattt
    11161 atgctgccag cccaggagag tggaagatgt tgtacctgac gaaaataagc aatgcgggga
    11221 gtgaattcac cgaaaacgag caaaaaatcg ctgactttct gcaggccaac gtgagtgagc
    11281 tgcaatccgt ttcttcacgg cagatggcga aacagctcgg cattagccag tcgagcatag
    11341 tgaaatttgc ccaaaaactc ggcgcgcaag gatttactga attgcgtatg gcgttaatag
    11401 gcgaatacag tgctagccgg gagaaaacga ataccacggc gctgcacctg cacagttcga
    11461 ttaccagtga tgattcgctg gaagtgattg cccgcaaatt gaatcgcgaa aaagagctgg
    11521 cgctggagca aacctgtgcg ttgttcgact acgcgcggct gcaaaaaatc attgaggtca
    11581 tcagtaaagc accgtttatc cagataacgg gcctgggcgg atcggcgctg gtggggcgcg
    11641 atctgtcgtt caaactgatg aaaattggct atcgcgtggc ctgcgaagcc gacacgcacg
    11701 tacaagccac cgtttcccag gcgttgaaaa aaggcgatgt acaaatcgct atctcttaca
    11761 gcggtagtaa aaaagagatt gtcttgtgcg tagaagcggc acgaaagcag ggggcaacgg
    11821 tgattgccat tacttctctg gctgactctc cgctgcggcg gctggcgcat ttcacgctcg
    11881 ataccgtttc cggtgaaacc gaatggcgta gctcgtcgat gtctacccgc acggcgcaaa
    11941 attctgttac cgatttactg tttgttggtc ttgtgcaact caatgatgtg gcgtcattga
    12001 aaatgattca gcgcagcagt gagctaacac aacgcctgaa atgatgcata aagcagcgac
    12061 tggattgaga ttttcctgaa ttagtgagct gatccgcagc aatattttgt ttatcctgta
    12121 ttttcagagg gaatggagtg taacgctctg tattaacaag gagagcatta aaatgggtaa
    12181 actcacgggc aagacagcac tgattacggg cgcattgcag ggaattggcg aaggaattgc
    12241 cagaactttt gcacgtcatg gcgcgaacct aatcttgctg gatatctccc ctgagatcga
    12301 aaagctggcg gacgaactgt gtggtcgtgg tcatcgctgt acggcggttg tcgccgatgt
    12361 gcgtgacccg gcgtcggtag ccgcagctat caaacgcgcg aaggaaaaag aagggcgcat
    12421 tgatatcctg gtgaataacg caggcgtttg tcgtctgggc agtttcctcg atatgagcga
    12481 tgacgatcgc gatttccata ttgatatcaa tattaaaggc gtatggaacg tcacgaaggc
    12541 ggtgctgccg gagatgattg cgcgcaaaga tggtcgcatt gtgatgatgt cttcagtcac
    12601 tggtgatatg gtggccgatc ctggcgaaac ggcgtacgcc ttaacgaaag cggcgattgt
    12661 tggcctgaca aaatcgctgg cggtggagta cgcgcagtct ggtattcgcg ttaacgccat
    12721 ttgcccggga tacgtgcgca caccaatggc ggaaagcatt gcccgccagt cgaacccgga
    12781 agatccagag tcggtgctga ctgaaatggc gaaagcaatc ccgatgcgtc gcctcgccga
    12841 tccgctggaa gtcggcgaac tggcggcctt cctcgcatcg gatgaatcca gctatttaac
    12901 cggtacacag aatgtgattg atggcggcag cacactgccg gagacggtta gcgtcggtat
    12961 ctgattcacc tctgtttcct ccctgcatgt gtggggagga tttcgtcttg aactaagttc
    13021 accaggctat tttatttgtc attttggccc cgggcagtgc tcgaaatact cacgtactat
    13081 gtgtacgctc cggtttctcc gcgctgtccg tgtccaaact gcccgcaaca attacgcctg
    13141 ttgaaccaag ttcttattcc ctttttaact tccaaatcac caaacggtat ataaaaccgt
    13201 tactcctttc acgtccgtta taaatatgat ggctattaga aagtcattaa atttataagg
    13261 gtgcgcaatg gccgttaact tactgaaaaa gaactcactc gcgctggtcg cttctctgct
    13321 gctggcgggc catgtacagg caacggaact gctgaacagt tcttatgacg tctcccgcga
    13381 gctgtttgcc gccctgaatc cgccgtttga acagcaatgg gcaaaagata acggcggcga
    13441 caaactgacg ataaaacaat ctcatgccgg gtcatcaaaa caggcgctgg caattttgca
    13501 gggcttaaaa accgacgttg tcacttataa ccaggtgacc gacgtacaaa ttctgcacga
    13561 caaaggcaag ctgatcccgg ctgactggca gtcgcgcctg ccgaataata gctcgccgtt
    13621 ctactccacc atgggcttcc tggtgcgtaa gggcaacccg aagaatatcc acgactggaa
    13681 cgacctggtg cgctccgacg tgaagctgat tttcccgaac ccgaaaacgt cgggtaacgc
    13741 gcgttatacc tatctggcgg catggggcgc agcggataaa gctgacggtg gcgacaaagc
    13801 caaaaccgaa cagtttatga cccagttcct gaaaaacgtt gaagtgttcg atactggcgg
    13861 tcgtggcgcg accaccactt ttgccgagcg cggcctgggc gatgtactga tcagcttcga
    13921 gtcggaagtg aacaacatcc gtaaacagta tgaagcgcaa ggctttgaag tggtgattcc
    13981 gaaaaccaac attctggcgg aattcccggt ggcgtgggtc gataaaaacg tgcaggccaa
    14041 cggtacggaa aaagcagcaa aagcctacct gaactggctc tacagcccgc aggcgcagac
    14101 catcatcacc gactattact accgcgtaaa taacccggaa gtcatggaca aactgaaaga
    14161 taaattcccg cagaccgagc tgttccgcgt ggaagacaaa tttggctcct ggccggaagt
    14221 gatgaaaacc cacttcacca gcggcggcga gttagacaag ctgttagcgg cggggcgtaa
    14281 ctgatgtttg cagtttcatc aagacgcgtg ctgccgggct ttaccttaag cctcggcacc
    14341 agtctgctgt ttgtgtgcct gattttgctg ctgccgctct ccgcgctggt gatgcagctg
    14401 gctcagatga gctgggcgca gtactgggag gtgatcacca acccacaagt ggtcgcggcc
    14461 tacaaagtga cgctgctgtc ggcgtttgtg gcatcgattt ttaacggcgt tttcggtctg
    14521 ctgatggcgt ggattttaac acgttatcgc tttccgggcc gcacgctgct tgatgcgctg
    14581 atggatttac cctttgcgct gccaacggca gtcgccggtt taacgctggc ctcgctcttt
    14641 tccgtaaacg gtttttacgg tgaatggctg gcgaagtttg atatcaaagt cacctatacc
    14701 tggcttggta ttgcggtggc gatggccttt accagcattc cgtttgtggt gcgtaccgtg
    14761 cagccggtgc tggaagagtt aggcccagaa tatgaagaag cggcggaaac gcttggtgca
    14821 acgcgctggc agagtttctg caaagtggtg ctgccggaac tttctccagc gctggtggcg
    14881 ggcgtggcgt tgtcgtttac ccgtagtctt ggtgagtttg gcgcggtgat atttatcgcc
    14941 gggaacatcg cttggaagac ggaagtgacg tcgctgatga tttttgtgcg cttgcaggag
    15001 tttgattacc cggcggcgag cgcgattgct tcggtgatcc tcgcggcatc cctgctgctg
    15061 ctgttctcaa ttaacactct gcaaagtcgc tttggtcggc gtgtggtagg tcattaatgg
    15121 cggaagttac ccaattgaag cgttatgacg cgcgcccgat taactggggc aaatggtttc
    15181 tgattggcat cgggatgctg gtatcggcgt tcatcctgct ggtgccgatg atttatatct
    15241 tcgtacaggc gttcagcaaa gggctgatgc cggttttaca gaatctggcc gatccggaca
    15301 tgctgcacgc catttggctg acggtgatga ttgcgctgat tgccgtaccg gtgaacctgg
    15361 tgttcggcat tctgctggcg tggctggtaa cgcgctttaa cttccccgga cgccagttac
    15421 tgctgacgct actggacatt ccgtttgccg tatcgccggt agttgccggt ctggtgtatc
    15481 tgctgttcta cggctctaac ggtccgctgg gcggctggct ggatgaacat aatttgcaga
    15541 ttatgttctc ctggccggga atggtgctgg tcaccatctt tgtgacctgt ccttttgtgg
    15601 tgcgcgagct ggtgccggtg atgttaagcc agggcagcca ggaagacgaa gcggcgattt
    15661 tgcttggcgc gtctggctgg cagatgttcc gtcgcgtcac attgccgaac attcgctggg
    15721 cgctgctgta tggtgtggtg ctaaccaacg cccgcgcgat tggcgagttt ggtgcggtgt
    15781 cggtggtttc cggctcgatt cgcggcgaaa cactttcgct gccgttacag attgaattgc
    15841 tggagcagga ctacaacacc gtcggctcct ttaccgccgc cgcgctgttg acgctgatgg
    15901 cgattatcac cctgttttta aagagtatgt tgcagtggcg tctggagaat caggaaaaac
    15961 gcgcgcagca ggaggagcat catgagcatt gagattgcca atattaagaa gtcgtttggt
    16021 cgcacccagg tgctgaacga tatctcactg gatattcctt ctggtcagat ggtcgcgctg
    16081 ctggggccgt ccggttccgg aaaaaccacg ctgctgcgca ttattgccgg gctggagcat
    16141 caaaccagcg ggcatattcg cttccacggt accgacgtga gccgcctgca cgcacgcgat
    16201 cgtaaagtcg gtttcgtgtt ccagcattac gcgctgttcc gtcatatgac ggtgttcgac
    16261 aatatcgctt ttggcctgac ggtgctgcca cgtcgcgagc gcccgaatgc cgcagccatc
    16321 aaagcgaaag tgacaaaatt gctggagatg gtccagcttg cgcatctggc ggatcgttat
    16381 ccggcacagc tttccggcgg ccagaaacag cgcgtggcgc tggcgcgcgc gctggctgtt
    16441 gaaccgcaaa ttctcctgct tgatgaaccg tttggcgcgc tggatgcgca ggtgcgtaaa
    16501 gagctgcgtc gctggctgcg tcaactgcat gaagagctga aattcaccag cgtgttcgtg
    16561 acccacgacc aggaagaagc gaccgaagta gctgatcgtg tagtggtgat gagccagggc
    16621 aatatcgagc aggctgatgc gccggaccag gtatggcgcg aaccagcgac ccgatttgtg
    16681 ctcgaattta tgggcgaagt gaaccgcctg cagggaacca ttcgcggcgg gcagttccat
    16741 gttggcgcac atcgctggcc gctggggtat acgcctgcgt atcaggggcc ggtggatctc
    16801 ttcctgcgcc cgtgggaagt ggatatcagc cgccgtacca gtctcgattc gccgctgccg
    16861 gtacaggtac tggaagccag cccgaaaggt cactacaccc aattagtggt gcagccgctg
    16921 gggtggtaca acgaaccgct gacggtcgtg atgcatggcg acgatgcccc gcagcgtggc
    16981 gagcgtttat tcgttggtct gcaacatgcg cggctgtata acggcgacga gcgtatcgaa
    17041 acccgagatg aggaacttgc tctcgcacaa agcgcctgat aggttgagtg aatgttaaac
    17101 gcccggaggc gcttcccgcg atccgggctt tttaatggca aggtttgtaa cctgtagacc
    17161 tgataagacg cgcaagcgtc gcatcaggca acaccacgta tggatagaga tcgtgagtac
    17221 attagaacaa acaataggca atacgcctct gataaagttg cagcgaatgg ggccgaataa
    17281 cggcagtgaa gtctggttga aactggaagg caataacccg gcaggttcgg tgaaagatcg
    17341 tgcggcactt tcgatgatcg tcgaggcgga aaagcgcggg gaaattaaac cgggtgatgt
    17401 gttaatcgaa gccaccagtg gtaacaccgg cattgcgctg gcaatgattg ccgcgcttaa
    17461 aggctatcgc atgaaattgc tgatgcccga caacatgagc caggaacgcc gtgcggcgat
    17521 gcgtgcttat ggtgcggaac tgattcttgt caccaaagag cagggcatgg aaggtgcgcg
    17581 cgatctggcg ctggagatgg cgaatcgtgg cgaaggaaag ctgctcgatc agttcaataa
    17641 tcccgataac ccttacgccc attacaccac taccgggccg gaaatctggc agcaaaccgg
    17701 cgggcgcatt acccattttg tctccagcat gggaacaacc ggcactatca ccggcgtttc
    17761 acgctttatg cgcgaacaat ccaaaccggt gaccattgtc ggcctgcagc cggaagaagg
    17821 cagcagtatt cccggcattc gccgctggcc tgcggaatat ctgccgggga ttttcaacgc
    17881 ttctctggtg gatgaggtgc tggatattca tcagcgcgat gcggaaaaca ccatgcgcga
    17941 actggcggtg cgggaaggaa tattctgtgg cgtcagctca ggcggcgcgg ttgctggagc
    18001 actgcgggtg gcaaaagcta accctggtgc ggtggtggta gcgatcatct gcgatcgtgg
    18061 cgatcgctac ctctccaccg gagtgtttgg ggaagagcat tttagccagg gggcggggat
    18121 ttaagattat tgcgcaggca ccgttttatc cgtctgcgca attagcgcat gtaaaaaagg
    18181 ctgtgcgttc tcatcgctct taccggggat cttcacaata tacggcttcc cggtaatcga
    18241 tgacgacgaa gcaaccttat caataaactg ctcggcagtg tcaatgcggt tacgggtatt
    18301 gccgagcttc agacgcagat gagaaaccgc ttcatcgcag gtatgttcat caccgttgcg
    18361 cacaaatatc aaatcctttt tctgtgataa tccctccagc atggcgttga tgcgggcttc
    18421 ttcatgggca gttaacttcg cgtaagcggg gagtgtcatt agtagtgtaa tgaccagaca
    18481 aatgattttc ttcattgaat taccttatcc ttgttttgta atagccgatt tgactgccag
    18541 aatcaggtaa agtttcggtt ggaatacgat gaggatcatc ggcaaggaga aaaaattaaa
    18601 tgagtagttt gttgttgttt aacgataaga gcagggcgct gcaggcggat atcgtcgccg
    18661 tgcagtcgca ggtggtttac ggcagcgtgg gcaacagcat tgccgtgcct gctatcaaac
    18721 agaacggtct gaatgttttt gccgtgccga cggtattgct gagcaatacg ccgcattatg
    18781 acactttcta cggtggtgcg attccggatg aatggtttag cggttatttg cgtgcgcttc
    18841 aggagcgtga tgcgctgcgc cagctgcgcg ctgtaaccac gggctatatg ggaacggcat
    18901 cgcaaatcaa aatccttgcc gagtggctga ctgcgctacg caaagaccat cctgacctat
    18961 tgatcatggt cgatccggtg attggcgata ttgatagcgg aatttatgtc aaacctgacc
    19021 ttcccgaggc gtatcgacaa tatttactgc cgctggcgca gggaattacc cccaatatct
    19081 ttgagctgga aatcctgacc ggtaaaaatt gccgcgatct cgacagtgcc attgctgccg
    19141 caaaaagtct gctttcagac acattaaaat gggtggtgat taccagcgcc tccggtaatg
    19201 aagaaaatca ggagatgcag gtggtggtgg tcagtgccga cagcgtgaat gtcatttccc
    19261 attcacgggt aaaaaccgac ctgaaaggga ctggcgacct gttttgtgct cagcttatca
    19321 gtggtttgct gaaagggaag gcgttaaacg atgcggtgca ccgtgcgggg ttgcgcgtac
    19381 tggaagtgat gcgctacacc cagcagcatg agagcgatga attgattttg ccgccgctgg
    19441 cggaagcata aaaaatg
//
LOCUS       DAJVTY010000071        19403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVTY010000071 DAJVTY010000000
VERSION     DAJVTY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19403
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.071"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(33..1862)
                     /gene="glmS"
                     /locus_tag="OQQ66_002209"
     CDS             complement(33..1862)
                     /gene="glmS"
                     /locus_tag="OQQ66_002209"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1905128.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2024..3394)
                     /gene="glmU"
                     /locus_tag="OQQ66_002210"
     CDS             complement(2024..3394)
                     /gene="glmU"
                     /locus_tag="OQQ66_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1905129.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3745..4164)
                     /gene="atpC"
                     /locus_tag="OQQ66_002211"
     CDS             complement(3745..4164)
                     /gene="atpC"
                     /locus_tag="OQQ66_002211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1905130.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4185..5567)
                     /gene="atpD"
                     /locus_tag="OQQ66_002212"
     CDS             complement(4185..5567)
                     /gene="atpD"
                     /locus_tag="OQQ66_002212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1905131.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5594..6457)
                     /gene="atpG"
                     /locus_tag="OQQ66_002213"
     CDS             complement(5594..6457)
                     /gene="atpG"
                     /locus_tag="OQQ66_002213"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1905132.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6508..8049)
                     /gene="atpA"
                     /locus_tag="OQQ66_002214"
     CDS             complement(6508..8049)
                     /gene="atpA"
                     /locus_tag="OQQ66_002214"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1905133.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8062..8595)
                     /gene="atpH"
                     /locus_tag="OQQ66_002215"
     CDS             complement(8062..8595)
                     /gene="atpH"
                     /locus_tag="OQQ66_002215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1905134.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8610..9080)
                     /gene="atpF"
                     /locus_tag="OQQ66_002216"
     CDS             complement(8610..9080)
                     /gene="atpF"
                     /locus_tag="OQQ66_002216"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1905135.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9142..9381)
                     /gene="atpE"
                     /locus_tag="OQQ66_002217"
     CDS             complement(9142..9381)
                     /gene="atpE"
                     /locus_tag="OQQ66_002217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1905136.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9428..10243)
                     /gene="atpB"
                     /locus_tag="OQQ66_002218"
     CDS             complement(9428..10243)
                     /gene="atpB"
                     /locus_tag="OQQ66_002218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1905137.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10252..10632)
                     /gene="atpI"
                     /locus_tag="OQQ66_002219"
     CDS             complement(10252..10632)
                     /gene="atpI"
                     /locus_tag="OQQ66_002219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1905138.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11249..11872)
                     /gene="rsmG"
                     /locus_tag="OQQ66_002220"
     CDS             complement(11249..11872)
                     /gene="rsmG"
                     /locus_tag="OQQ66_002220"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1905139.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11936..13825)
                     /gene="mnmG"
                     /locus_tag="OQQ66_002221"
     CDS             complement(11936..13825)
                     /gene="mnmG"
                     /locus_tag="OQQ66_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1905140.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14204..14647)
                     /gene="mioC"
                     /locus_tag="OQQ66_002222"
     CDS             complement(14204..14647)
                     /gene="mioC"
                     /locus_tag="OQQ66_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1905141.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14737..15195)
                     /gene="asnC"
                     /locus_tag="OQQ66_002223"
     CDS             complement(14737..15195)
                     /gene="asnC"
                     /locus_tag="OQQ66_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1905142.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15347..16339
                     /gene="asnA"
                     /locus_tag="OQQ66_002224"
     CDS             15347..16339
                     /gene="asnA"
                     /locus_tag="OQQ66_002224"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1905143.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVIHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16344..17795)
                     /gene="viaA"
                     /locus_tag="OQQ66_002225"
     CDS             complement(16344..17795)
                     /gene="viaA"
                     /locus_tag="OQQ66_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1905144.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWSEALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17789..19285)
                     /gene="ravA"
                     /locus_tag="OQQ66_002226"
     CDS             complement(17789..19285)
                     /gene="ravA"
                     /locus_tag="OQQ66_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1905145.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4638 a         5124 c         4863 g         4778 t
ORIGIN      
        1 ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccaag
       61 ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc
      121 agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg
      181 tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata caactgaccg
      241 ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc gttgttcggt
      301 gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg tttcagctcg
      361 ccagcagcgt aggcttcagc gtgaatgtag gagatctctt tcagcttcaa tgcgccttcc
      421 agtgcgattg ggtactgatc gccacggccc aggaacagtg cgtgatgttt gtcagagaaa
      481 tcttctgcca gagcttcaat gcgtttgtcc tgagatagca tctgctcaat acggctcggc
      541 agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga
      601 gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggac
      661 gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga
      721 gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga
      781 cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg
      841 ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac gtcgcacgga
      901 atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata agaagtacca
      961 caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc
     1021 tcgcttaaat caacctgacc gtggctgatg cgcccagtaa gggtgttttt gatcgcgttc
     1081 ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca
     1141 tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg
     1201 tttaccgaac ggcgagtaat ttccgcaata tcgccctctt caaggaagat aaagcgacgg
     1261 gtcaccggca gcagcgccag ctggtcagaa gcgataaagt tttcgcccat acccaggcca
     1321 atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg
     1381 atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc ctcacgcaga
     1441 gtcccgcctt gttttagctc ccagttcacc agatgggcaa tcacttcggt gtcggtttca
     1501 gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg gttttcgatg
     1561 atgccgttat gcaccaccat aatgtgttca gaaacatgcg gatgcgcgtt agcttctgaa
     1621 ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg cagaggatgt
     1681 tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg ggtcatatga
     1741 ccttccgcat caacaacggc cagaccagca gagtcatatc cgcggtattc cagacgacgt
     1801 aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac
     1861 atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc
     1921 gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt
     1981 atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt ttctttaccg
     2041 gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc
     2101 cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg
     2161 gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt
     2221 tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa
     2281 tttccgcatc gcccaggtaa gtcagatgac cagctttcga gcctttaccc agacgcgctt
     2341 ttttcatctc aacaaagtta ccgacgtgtg caccttccag caactcagca ccaggacgca
     2401 gacgggcaaa cgggccaata gtacaggccg ctgccagatt cacgtcttcc acaacggtat
     2461 acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa ccggtgccga
     2521 ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa
     2581 catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa
     2641 cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga
     2701 gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga acggcgacga
     2761 tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag
     2821 cattattgtt ggtcagcttc gccagccagc gtttcatatc tgcgccgtta gagatcagaa
     2881 tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa
     2941 tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt
     3001 tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc acgcaggcgc tggagtgttt
     3061 cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga
     3121 aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt
     3181 taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaggt
     3241 gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc
     3301 cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg
     3361 caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac
     3421 gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg
     3481 ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca
     3541 cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt
     3601 tgctcttatt tcgatcaatg aataaacggg aaaacagggg tgttttatat cagcaggatc
     3661 tatgtgaacg ctcttcagga cggatcacgc gtaaaaaaaa accagcctgt ttccagactg
     3721 gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag
     3781 ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga
     3841 gctgctaatg tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc
     3901 ctggccgcga attgcggtgt cggccagaac ggtcacgttg ccagactgca cttcaagaat
     3961 gccgccagac agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc
     4021 aggcttaatg gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct
     4081 acccgttacc tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac
     4141 gtccaggtgg taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct
     4201 ttttccacag cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg
     4261 tattcgcctt ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta
     4321 cccggagaac cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc
     4381 ttacgagcac gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaggatg
     4441 gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg
     4501 tcgtagtgtt cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg
     4561 tcaacggccg ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca
     4621 aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt catccgcagg tacgtatacc
     4681 gcctgtacgg aggtgataga accagttttg gtggaggtga tacgttcctg cagaacgccc
     4741 atctcttccg ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg
     4801 gatacttccg taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca
     4861 cgaccttcgt cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg
     4921 tttcccggcg gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag
     4981 tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac
     5041 acagagtaac cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt
     5101 ttacctacac ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc
     5161 aggtcgataa ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag
     5221 gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg
     5281 accggttcac ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg
     5341 atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt
     5401 gcgatggtac gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca
     5461 ttaccatttt gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg
     5521 acgtcaacta cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta
     5581 cgaaataacc tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa
     5641 tgctggcctg acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat
     5701 tgtcggtcgc ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa
     5761 ccacgccctg ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct
     5821 tcggatcggg ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg
     5881 ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt
     5941 tgctgacaat gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac atcactttta
     6001 ccggaccgat cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa
     6061 cattgccgcc cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc
     6121 attgaacgcc tttgtcggtc caggtcttca tttccgccag cagttttttg aacaggttaa
     6181 tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa
     6241 cgtcgcggtc ttccaggtaa aggtgcttat attccagatt accatgtgca aggtgaccaa
     6301 tcactttgcg catggtttct gcataaggac ggctggccgc catgcgatcc tgcgatttac
     6361 gcattttgga agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc
     6421 ttgcgatctt actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg
     6481 gcctgcccta aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag
     6541 gatgcctttc agtttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg
     6601 catcaacgga gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccaat
     6661 tttcgacagt tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg
     6721 ctgcgcaacg gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc
     6781 gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc
     6841 cagttcacga tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt
     6901 ggtctgtgct gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat
     6961 accggcgttg aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt
     7021 ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttcg ataatcggca gtgcggtcag
     7081 agaaccggtt ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac
     7141 acgtgcagca cgctccagca gacgagagtg gaggtagaaa acgtcgcccg ggaatgcttc
     7201 acgtcctggc ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga
     7261 caggtcatcg taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc
     7321 gcaaccggca tacggtgcca ggtattgcag tgcagcggat tcagacgcgg ttgctaccac
     7381 aacgatggtg ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt
     7441 ggacgctttc tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat
     7501 gatggcatcg atagccagtg cggttttacc tgtctgacgg tcaccgatga tcaattcacg
     7561 ctgaccacga ccgattggga tcatggagtc aacggcttta taaccggtct gtaccggctg
     7621 atctacggac tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc
     7681 gtgatccagc ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag
     7741 caggccacgg ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc
     7801 ggcaaggtca gcgtacggac ccataacaac cgcacctaca gagtcgcgct cgaggttcag
     7861 tgcgatagcg taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag
     7921 gccgtgaatg cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc
     7981 actcacaaca ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt
     8041 cagttgcatg ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc
     8101 gcgtacgctg ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga
     8161 cttatcgatt ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga
     8221 aattttcgcg agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt
     8281 agcctcactc acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag
     8341 acgaccattt tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc
     8401 acaaactgcg ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc
     8461 tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc
     8521 tacactttgg tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt
     8581 aataaattca gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg
     8641 tcgctgttag cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc
     8701 aggatagcaa cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct
     8761 gcctgcgcct gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc
     8821 agaatctgcg agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct
     8881 tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct
     8941 gcggaagcaa ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc
     9001 ggccatacgt acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg
     9061 attgttgcgt taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc
     9121 tttaaataaa agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac
     9181 agcgatcatc gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag
     9241 aggaatcaga tcaggttgac gcgctgcgcc ttccaagaat ttacccccga ggatgccgat
     9301 accgatcgca gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag
     9361 atccatattc aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt
     9421 tggtaaatta atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga
     9481 tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc
     9541 actgtgacca ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca
     9601 tgttaccgaa cagtcgcaaa ccgagtgaaa ctggtttgga cagcaggctt accccttcaa
     9661 ggattaagtt gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg
     9721 tgaagccgcc gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca
     9781 atgccataga cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac
     9841 ccagtacatg ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca
     9901 tcaggaatac ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat
     9961 ggtacatgtc tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa
    10021 acttacctgg cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca
    10081 acagacccag caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg
    10141 gggggttttg tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt
    10201 ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt
    10261 gttgttaatt acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat
    10321 cagcggcaag aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc
    10381 cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt
    10441 atgcgcctgg tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag
    10501 gcccccgctt attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact
    10561 tgctatcacc accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag
    10621 cgacacagac atcacgtttt ttactcctgc tcccttcgag gtatgccgcg tgtcgtataa
    10681 aactttcttt aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggcgcgcccc
    10741 cgtgatttca aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc
    10801 atacaattgc gacttttcta ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta
    10861 aacactggtg ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagcatatt
    10921 tatttacttg gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac
    10981 tgttttaata aatgacattt acacaacaaa aaccacccat tgacattttt aataatgttt
    11041 tagcagccaa tgatggttct tagcgccgat ttttagcaga ctgatatttt cactaatgac
    11101 ttattttctg cttaccaaaa aaagccacgt tatcttgttg atgcaaaaga gtgaacgtgg
    11161 cgttaaatgt aaccagttat atcagtagaa aacctggttg ttgttaacag tctaaccggt
    11221 caatttttta tgattttttt gataaaaatt aaattttatt tgctttaatc accaccagat
    11281 gacgttcgcc atccagggct ggaacctgaa gtttaaccac tgattcgacc tgatattctt
    11341 cgggcaacaa agcgatttca tcttccggca tttgcccttt cagcgcgtag aaacggcctt
    11401 gctcaccagg aagatggtgg caccagctca ccatatcgtt cagagaggca aaagcgcggc
    11461 taattacgcc atcaaatggc ggctctgaag gaaactcttc taccctgctc tgtacaggtt
    11521 caatattctc cagtttaagc tcatgttgca cctgacgaag gaaacgcacg cgtttaccaa
    11581 ggctatccaa cagagtgaaa tgggcttcag gacgcacgat agagagtgga atgcctggca
    11641 gtcctggtcc ggtgccgaca tcgataaacc gttcaccttg cagatacggt gccaccacaa
    11701 tgctatcgag aatatggcgt accagcatct cattaggatc gcggaccgaa gtcaggttgt
    11761 acgctttgtt ccatttatgc agcatattca cgtaggcaat aagctggttt ttctggtgat
    11821 cggtaagcga aatacctgcg tctttcagca gtaaggagag tttgttgagc acggtgatta
    11881 cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca ggcattttta atgcgttatg
    11941 cgctacgacg cagcataccc tgttttttca gccacaccag cagaatggag atagccgcag
    12001 gcgtgacgcc agaaatacgc gaagcttggc cgatagaggc tggtttgtga tcgttaagtt
    12061 tggcgatcac ttcgttagaa agaccggata cctggcggta atccagtgtc gcgggtagca
    12121 gggtgttctc gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg atataacctt
    12181 cgtatttaac ctgaatctca acctgttccg ccgcctgttc gtctgtcaac gcaggggcaa
    12241 acggcgtcag cgtggttaat ttttcataag tcatttccgg acgacgcagc agatcttcac
    12301 cactggcttc acgggaaagc ggcgcagtca ggtgagcatt cacttcggct gcagcttccg
    12361 ccgacggggt tacccaggtc gatttcagac gctgacgctc acgctcgata ttctcaagtt
    12421 tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga ccgatttcag
    12481 tcaaacgcag atccgcatta tcttcgcgta gcatcagacg atattctgcg cgcgaagtaa
    12541 acatacgata cggttctttg gttcctaaag tgcacaggtc atcaactagt acgccgagat
    12601 acgcctgaga acgtgccgga gcccaacctt ctttgtcagc agacagacgg gcagcgttaa
    12661 gaccggccag caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg ttaatctgac
    12721 cagcaaagaa cagcccctgg ataaacttgc tctccagcgt cggtttcagg tcgcgaggat
    12781 cgaagaagtc atactcaatg gcataacccg gacgcacgat cttcgcgttt tccatcccct
    12841 gcatagagcg gacgatttgc atctgcacat cgaacggcag gctggtggag ataccgttcg
    12901 gataaatttc attagaggtc agtccttccg gttcaaggaa gatctgatgc tgatttctgt
    12961 cggcgaagcg catgactttg tcttcgatcg acgggcagta gcgtgggccg acaccttcga
    13021 tcacccctgc atacattggg ctacgatcga ggttactgcg gatcacatca tgggttttct
    13081 cgttggtatg agtgatataa cacggcacct gctggggatg ctgggacgca ttgcccataa
    13141 acgagaatac cggcattggg ttatcgccat gctgttgcgc cagcacacta aagtcgatag
    13201 ttcgagcatc aatacgcggt ggtgtcccgg ttttcagacg accaacgcgc agcggcagtt
    13261 cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc agcacggcca ccgctgtaat
    13321 tatccagacc gatatgaatt ttaccgtcga ggaacgtccc aacggtgagc acgacggctt
    13381 tggcacggaa cttcagtccc atttgggtaa cagcaccgac cacgcgatcg ttttcgacaa
    13441 taagatcttc aaccgcctgc tggaagatca tcaggttcgg ttggttctcc agcgccgtac
    13501 gtaccgcctg acggtagagc acacgatccg cctgagctcg ggtagcgcga accgccggtc
    13561 ctttgcttgc gtttagtatc ctaaactgga tacccgcttg atcgatcgct ttcgccatca
    13621 gaccgccgag tgcatccact tcttttacca gatgtccctt cccaataccg ccgatcgccg
    13681 ggttgcagct catctgcccc agagtgtcga tattgtgtgt caaaagcaga gtctgttgac
    13741 ccatacgcgc cgcggccatc gcggcctcgg tgcctgcatg acccccgcca atgatgatga
    13801 cgtcaaaagg atccggataa aacatggtga ttgcctcgca taacgcggta tgaaaatgga
    13861 ttgaagcccg ggccgtggat tctactcaac tttgtcggct tgagaaagac ctgggatcct
    13921 gggtattaaa aaggagatct atttatttag agatctgttc tattgtgatc tcttattagg
    13981 atcgcactgc cctgtggata acaaggatcc ggcttttaag atcaacaacc tggaaaggat
    14041 cattaactgt gaatgatcgg tgatcctgga ccgtataagc tgggatcaga atgaggggtt
    14101 atacacaact caaaaactga acaaccgttg ttccttggat aactaccggt tgaaccaagc
    14161 ttcctgacag agttatccac agtagaacgc acgatctgta tacttatttg agtaaattaa
    14221 tccacgatcc cagccattct tctgccggat cttccggaat gtcgtgatca agaatgttga
    14281 tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt gagttctgcc tcgagtttat
    14341 cgatagcccc acaaaaggtg tcatattcac gactaccaat accgattgcg ccaaagcgga
    14401 ccgcagaaag atcgggcttc tgttcctgca atgcttcata gaaaggagaa aggttgtccg
    14461 gaatatctcc ggcaccgtgg gtggagctga taatcagcca gatccccgag gcagataaat
    14521 cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc cgcctcttcc agcttttcag
    14581 ccaggtgttc tgctacatat tcggcaccgc cgagggtgct gccgctgata agagtgatat
    14641 ctgccataaa ccgccacctt tattaagagt ggcgtattgt acgctgtgaa cgcgttggga
    14701 tctacctgtg gaaaagtatg ggattaaaaa agccgatcag ggcttgatag tacgcatgat
    14761 cgggtgttgc aggacgatca atgtctcggt ggactgaatt tcatcaattg tttggatctt
    14821 gttgataagt acatgctgga gagcgtcgat cgaacggcac attactttta taaagatgct
    14881 gtagtggcct gttgtgtaat aggcttcagt gacttcatca aggctttcca gctttgccag
    14941 cgcggaaggg tagtctttgg cgctctttaa tataatgccg ataaagcagc ctacgtcata
    15001 accgagctgc ttcgggctga catcaatacg cgccccggta atgatccccg cctgcttcat
    15061 tttctctacc cgaacgtgaa tcgtccccgg actgacgcca aattgtttcg ccagttcggc
    15121 gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg ccacggtcca gattgtcgat
    15181 cagataattt tccataggat tttcttatgc agattgatga ttcattctat tttagccttc
    15241 ttttttaatg aatcaaaagt gtgttaggct ttttattgaa tgattattgc atgtgtgtcg
    15301 gtttttgttg cttaatcata agcaacagga cgcaggagta taaaaaatga aaaccgctta
    15361 cattgccaaa caacgccaaa ttagcttcgt gaaatctcac ttttctcgtc aactggaaga
    15421 acgtctgggg ctgatcgaag tccaggcacc gattcttagc cgtgtggggg atggcacgca
    15481 ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa gtgaaagctc tgcctgatgc
    15541 ccagttcgaa gtgattcatt cactggcgaa gtggaaacgt cagaccttag ggcaacacga
    15601 cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc cttcgccccg atgaagaccg
    15661 tctttctccg ttgcactcgg tctatgtaga ccagtgggac tgggaacgcg taatgggcga
    15721 cggtgagcgt caattctcga ctctgaaaag cacggtagag gcgatctggg cgggaattaa
    15781 agcaaccgaa gctgcggtta gcgaagagtt tggcctggca ccgttcctgc cggatcagat
    15841 ccacttcgta cacagccagg agttactgtc tcgttatctg gatctcgatg ccaaagggcg
    15901 tgagcgggcg atagcgaaag atcttggcgc ggtattcctt gtcgggattg gcggcaagct
    15961 gagcgatggt catcgccacg acgtgcgcgc accggattat gatgactgga gcaccccgtc
    16021 agagctgggt catgcgggtc tgaacggcga tattctggtg tggaaccctg tactggaaga
    16081 tgcgtttgag ctttcttcca tggggatccg cgtggatgcc gacacgctga agcatcagct
    16141 ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg catcaggcgc tgctgcgcgg
    16201 tgaaatgccg cagaccatcg gcggcggtat cggccagtct cgtttgacta tgctgctgct
    16261 gcaactgccg catatcggcc aggttcagtg tggagtatgg ccagctgctg ttcgcgagag
    16321 cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc tgagcaggcg gcttcgcatc
    16381 ccggtatcaa agcgccagat atgatcgaaa atgcgcatga tgccgggttt gccgtgtgcc
    16441 gacatcgcca cggcatgaaa gcgatgctga tgtacccgct gcagctcttt cactttactc
    16501 gtcacgtcgt caggcaaccg ctgagcgata aaatcagaaa tcaccaccgc atcggcatca
    16561 aaccattccc tgctttgcaa gcgttccata atggcgcgaa aacaactggc aagatcggtg
    16621 ccgccacgaa actgctggct taaaaaacgg attgcttgtt cgatgccttg tgggcctgaa
    16681 agctcataac ggacgatctc ggtggaaaat agcataatat agcagcgccg gttttctgcg
    16741 agagcaatgc gcatcaaggc caggcagaac gctttcgcac actgttcatt aaagccgccc
    16801 attgagccgg aagtatccac acagacaata aacggcccgc gcggctgttc atcgtaatct
    16861 ttatgtacca ccggacgttc gatcactttt tcacgccacg actcaccgtg caggcgatag
    16921 gtgagcaact gtttttccac cagccgacgg taaaactcat actccagttc cgttatccct
    16981 agtgtcgcca gttctggcgg caggagacgt aaaatatcat cgctttgttg cagaccatca
    17041 acctgctcag gaaccgtcgc cggttcgcgc accatggtgc ggaaggtttc catctgcgca
    17101 tcgttgcgcg gtattgattt ggcttcccga gaacgcccca gctgctctgc caggcgtttc
    17161 agttccggct gttcgttaag aaattcaccg tatttcacaa tcaactgata gtcgccacgt
    17221 ttaagctggc cggcgctcat atcccacaga cgaccagctg cggtattgtt atctgcgaga
    17281 atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa cttcacttaa cagttgttcg
    17341 cgttcttctt ctaatagctg ttgatttaac gtcgttgctt gcacgatcag acttaaacgc
    17401 cagcgctgga gaaaaagcgt gtgtaacgct gaagttattg tgctgttagc atcaaccaac
    17461 tgccgggctt gttctgccca tggggaattc agacgatgca gtaagtccag gatctgtggt
    17521 agctgcacaa taaactgcgg cgtggagagg agctggcttt gctgatagca catcacctct
    17581 tcggtgagtt ctggcgggac tcgggcatct ttcagccgac tgcgcagcgc ctcactccag
    17641 cggggaacat catcagtgat tgctgccttc agtcgtggga atttttcaaa gaagaccgcc
    17701 agctgcggtg aggccagcag tgcgatgatc atctcttcga tcaatccctc ttcgctgacg
    17761 gccagcatca cattaagcgt atccagcgtt agcattgttg tgcctggcga atctgtgcac
    17821 cgacatcctg taggctggct tcaatgcgac ctaaccagtc gccagggata aacagacatt
    17881 tttgctgttc gctgaataaa gcgtgttgct tacgccagtc gctttcaagc tcttccagtt
    17941 gttgttttat ttcacctggc agaccttcag ccgatgaacc gggaagtgcc agcgtactgc
    18001 cttgtaaact tacatcgcgt acaacaagat gttgggtgct atcaacttcc agattcagtt
    18061 tctgggcaaa gccgataccg ttcagtttgc cgcgaatttc accaccttta ctcagccact
    18121 gttccagcgc gctacgctca aaggagatat gaaccacttc catatcatgc agttttaacg
    18181 gtttttgcag cagcagagtc agagtggaag cagtaacgtt aacagggagt tgatactgct
    18241 gacgacggct aaaaatgccg cccagacgaa ttaccgttaa ggctgtttta tcgctttgtt
    18301 gctgctgaag ttgcaggtga cgttgcacaa tcgcgcctag gcgggtcaac atcccttgct
    18361 gttgccaggc gtgaccggtc atcaatacat caatttgttg ttgtatcaaa ttcaggcttt
    18421 gcgcgtcata ccacaggcaa tctttcagca aaatgagatc taccggggca acagcactgc
    18481 gaccgctaaa aaaggcgctg gcctgcaata atcggatcgc ttttttccag cgacgatccg
    18541 agacataagg cgcatccggt aatttatcca gttgctggcg cagcataaaa atcagctcaa
    18601 atacatgatc gggcagcgta atttcaccaa tctctttctg ccagcgttca tattcttcat
    18661 ctgtgacctg caaggcatca ggaaccggat tgtcgttttc atcctgttga ctggtcagca
    18721 tggagcggaa attcgcttta tcctgcactt tatctaacca cagacgaatc agcatgcggt
    18781 catataacgc ttccagactg ctgtctgctt ccggcagctc gttggaggcc gccaccagca
    18841 gacgcatcgg gattttttct acgtgtgcac cgttgcggaa ctggcgctcg ttaatggcgg
    18901 tgagcaaggt attaagaatt gccgggcccg ctttccagat ctcatccaga aagacaattt
    18961 ctgcttccgg caggtaaccg ctggttaaac gttcatagcg cccttcatct tttagcgcct
    19021 gaatagaaag gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc agatattcaa
    19081 acgcgcgggc attctgaaag gcgaatttta agcgccgggc gatcaaactt ttggcaatac
    19141 ctggcgggcc aaggaggaac acactttcac cacttaatgc cgctaataaa cacaagcgga
    19201 tggcgtggct acgttcataa agcccctttt ccaacgaact gctcaggcgg gaaattcttt
    19261 ccgctaataa atgagggtga gccataatga agtggcgtcc tttcgtcaaa agttctgcgt
    19321 aaattgcgag tatagacgtt tcttgctggt ggctaaaata gtctcaaagg ggggtaatgc
    19381 cagtcagtta agcaactgac tgg
//
LOCUS       DAJVTY010000072        19331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVTY010000072 DAJVTY010000000
VERSION     DAJVTY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19331
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.072"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(189..530)
                     /locus_tag="OQQ66_002227"
     CDS             complement(189..530)
                     /locus_tag="OQQ66_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1905146.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(533..4312)
                     /gene="tamB"
                     /locus_tag="OQQ66_002228"
     CDS             complement(533..4312)
                     /gene="tamB"
                     /locus_tag="OQQ66_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1905147.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4309..6042)
                     /gene="tamA"
                     /locus_tag="OQQ66_002229"
     CDS             complement(4309..6042)
                     /gene="tamA"
                     /locus_tag="OQQ66_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1905148.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6248..6886
                     /gene="msrA"
                     /locus_tag="OQQ66_002230"
     CDS             6248..6886
                     /gene="msrA"
                     /locus_tag="OQQ66_002230"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1905149.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7209..8552
                     /locus_tag="OQQ66_002231"
     CDS             7209..8552
                     /locus_tag="OQQ66_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1905150.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8614..8820)
                     /locus_tag="OQQ66_002232"
     CDS             complement(8614..8820)
                     /locus_tag="OQQ66_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1905151.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9145..9699
                     /locus_tag="OQQ66_002233"
     CDS             9145..9699
                     /locus_tag="OQQ66_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1905152.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(9789..10700)
                     /locus_tag="OQQ66_002234"
     CDS             complement(9789..10700)
                     /locus_tag="OQQ66_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905153.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
     gene            complement(10912..11652)
                     /gene="cysQ"
                     /locus_tag="OQQ66_002235"
     CDS             complement(10912..11652)
                     /gene="cysQ"
                     /locus_tag="OQQ66_002235"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1905154.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            11842..13785
                     /gene="cpdB"
                     /locus_tag="OQQ66_002236"
     CDS             11842..13785
                     /gene="cpdB"
                     /locus_tag="OQQ66_002236"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1905155.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(13914..14294)
                     /locus_tag="OQQ66_002237"
     CDS             complement(13914..14294)
                     /locus_tag="OQQ66_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1905156.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGGSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDEHAA"
     gene            14383..15243
                     /gene="qorB"
                     /locus_tag="OQQ66_002238"
     CDS             14383..15243
                     /gene="qorB"
                     /locus_tag="OQQ66_002238"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1905157.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            15351..16316
                     /locus_tag="OQQ66_002239"
     CDS             15351..16316
                     /locus_tag="OQQ66_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1905158.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            16424..17086
                     /gene="ytfE"
                     /locus_tag="OQQ66_002240"
     CDS             16424..17086
                     /gene="ytfE"
                     /locus_tag="OQQ66_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1905159.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(17131..18543)
                     /gene="cycA"
                     /locus_tag="OQQ66_002241"
                     /gene_synonym="dagA"
     CDS             complement(17131..18543)
                     /gene="cycA"
                     /locus_tag="OQQ66_002241"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1905160.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWTGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(18852..>19331)
                     /gene="fklB"
                     /locus_tag="OQQ66_002242"
     CDS             complement(18852..>19331)
                     /gene="fklB"
                     /locus_tag="OQQ66_002242"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1905161.1"
                     /translation="GKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEEN
                     AKKEGVNSTESGLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEF
                     PVNGVIPGWIEALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         4729 a         5122 c         4713 g         4767 t
ORIGIN      
        1 atactcaccg ttcaaagcgt gcccccgttt tacccctgca catttgtgaa cgccagcaga
       61 gttttttatg cgattgcaga tatggaaagc ggctcggaga gaaaagcaaa agatggaaaa
      121 gttacaaaaa aaaatcgcga gatacatcgc ataaaacaac gccacccgaa ggtggcgtgc
      181 ttttattatt acttatccct gtctaaccag tcgccgcttt caattagctt taatccctcg
      241 acgggtcgtt gataaacgta catccatgca ctcccgtacg gcgtctgaat caactggcgc
      301 gcgtattcac cgcccctggt gcgcaaggca tcaagttcgg ccagcgtggc gttgtcaata
      361 cgataaactt caccgtgtac cgttccgttc cccggaactg cgcctggata gtggcccagg
      421 ctatacaact ggtagttatc gatactgaaa tcgcccagta actgggcatt ggtcatccag
      481 tgactgttgc cttgtttgtg gcgtaaactg ccgtagacaa atattcgcat tgctaaaact
      541 cgaactgata gagcaaatcc agtgcctggt ctacaccaga cacggcttcc agatatagct
      601 taggcatcag gcgataacgt aacgtgagtg ttgctataga gtcaaatata cccacgccgt
      661 atttcacttg caggcctggc aatacatagc cgctgaccac tacctgggag gagtcgccta
      721 ctccctgggt gtcgagcgct aaattgctta cgccaaacgt ctcgccgatt ttacccacaa
      781 tctggccact ttgcgcaacc cccagaccaa tcagcatcga ggtcattgcc gcactgtcgc
      841 tctgatcgct ctccagccct tgtccacgta gcaaataaga caatgcagct tgttgcgaca
      901 tcgccgggtc agagaagatc tccgctttcg gttcgtccgc cagaccagtg acgcgaactc
      961 cggcgattac gtcgtcttct gtagcatccg ggttacgaat agcttcaata ttaagatacg
     1021 gttgatctgg cggaccagag aacagtagct cacccttacg cacaatcaga tcctgaccat
     1081 aggcatggaa gcgcccttca gggatgttga tctgcccgtt caggcccagc ccttgtttgt
     1141 cctgaacgac gttaagatcg cccgtcagcc gcgctttcag gccaaaggcg tcaatgcgca
     1201 cattgttgcc aacgtggaca atcaggttac tgttaatcgg aatcgacgcc gttttcggct
     1261 cttccggttg caggttatcg ttaagcatca ccacatcgct ggagacgcct actgcgcttt
     1321 ccggcagatc gtgcaccacg atgcgcgccc acgggacatc cacgcgacca tcgagggtaa
     1381 acaggtttgg tgtagcctcg aatacaacat ctggcgatac atccattcgt accatcggcg
     1441 gcacggtgat ccgcacttta ctgcctttcg ccgttactcg tgcccgccag ttttcaattt
     1501 ggctccagtc ggcatcaccg ttcaggtaga tttcaccctg ctgggtccgt actgtaccgg
     1561 caagcgtcga gcgcataccg ttaaagttga ccgcaagctg gctcggctgc atatcaaacg
     1621 gcataaagtt gccgtcgata tccacacccg taacctgaag ctgaccaaac aactgcgggc
     1681 tttgcacatc accacccaga cgcaagttgg cactcaccat ccccgctgct ttttccccac
     1741 gagtaaagat ggggtttatc atcgcaaggt tgaagttacg gatattgacg ttgccaccaa
     1801 gattacggcg gccttgcgga tcggtcacca gcacctgtcc atcaaactgg ccgttattgg
     1861 tcaggcggat ggtccagccc aattcggcac ggttgttacg caattccgcc gtcagattca
     1921 gtgtctgaaa cgccaccggc agcgccgcat cgttgacggt ttgcgttacc tgcacgttac
     1981 gccccgaaag ggtgatactg ccctgcggca gcccctcttt cgtggtgtcc caggcaacat
     2041 ccgctttacc cgtgaagata ccgctggcct gagtggtttc tggcataaac ggtttcagca
     2101 tggcgaagtc gaagcggttg agattcacca ccgcacgccc ttcggccccc gcatcgatag
     2161 tttgcggcac gcacagttcc gcattcgggt taagccagca gtgtggcccg atgctgattt
     2221 tttgctcctt attgcggtaa tccagcgcaa tatcgcgggt cagcgaccac gggccgacca
     2281 gcgtctggaa gcgggtattg ctaagagttc ccttccagcg ttcttctttg cgatcaaaac
     2341 ttcctgccag attaagctgc ccggagacag gctcgccctg aatccgcaac tgtagctcgt
     2401 gctgcttttc gctgcctttg gcattcaggg tgacgaggtt gatatttaca tccggctgcg
     2461 aaatttgctc aacgcgtacg tcgagtttcc cggcgatctg atcggtggat ttaatgtcgc
     2521 cttccacgcg aacctgcgtc acggaaagtt cctgccagcg caggccgcgc gcggtgatat
     2581 ctgccagtag ttgtggcgct tccaccgtgc cgcgtacttt caccagccct ttcgctgtac
     2641 cgccaagccc cggcagcgcg ttatccagcc ccggcgcgtt gatggtggca tcaagattga
     2701 gatcttttac ccccagctcg cctttcactt cggcactgtt tggccccagt tccagatgaa
     2761 gcccagggat catccactgc atataactgt tgcctttcag cgtgccgtca acgttcactt
     2821 tgttctgttt gacgttaccg gtcagcttca gttctggcac ctccatctgc caggtgccgc
     2881 cgtacaggct accgcgggtt ttaatcaagc cattgagttt cgacggccac tccgggaact
     2941 ctttggcggt gttaatgcca ttaagcgtta gctcaccgcg ccaactaatg gcctgctgcc
     3001 agtcgagcaa cgccttgagt tcagttttcc cttccagcgc cgcgacggtg agtttgtcga
     3061 gattgacctg ctgttcatta cctttggcat caagggtaat ggtggctggc gggatctcct
     3121 gtcccttcac tgccgtacgc atagagagcg tgtaatcggt cattttgccg gtaagtttca
     3181 gtttcagatc atccgcctga tactgcttct caccagtgaa cggccagtaa agctgtttgc
     3241 tgttcacttc cacgttgagc ggcaatccgg cttccgccag tcgcgtctgg gcgcgtaaat
     3301 ccatatccac cggaccggaa agattaacgc caatctccag ctgttcgcgc agcgcgccgc
     3361 ccacttttag cttcactttt tcacctttca acggctccac gttcagtgtg ctgttgaggg
     3421 tgatatccac cggccagttg tctgacagct gcgccgtgcc gctggcgttg acgatcccct
     3481 ggttggaatc gatatccagg gcgtccagtt tagtattgcc gtcaatgctg ctcactttca
     3541 gcagcatggt gcgcacggtg atgtccgtgt cgcccgtcac gcgtaactgc tcgcctttaa
     3601 actcttcaat gttcaggtta agcggcaaat gcaagtcggt catttccggc aataccgggc
     3661 gagaaaagag atctttcagc gtttcgccga gcggcttttc atccggctgc ggattttcaa
     3721 ttttcggttc gaccacttct tcctgcgcca cttccgccac tttcggcaga gcaatcagca
     3781 ggcctttcag cgacgtcggt ttcagggtca gggttttctc ctgccagttc aggccggagg
     3841 tgaagtccat caccgacacc gtggtgtcat caatcttgat gttgacgttg tccagcgcca
     3901 cccgtgtcag ggtgatggga tacggcgtgg agagatccag cggaccgcta tcttcctctt
     3961 cttcaacctg ttcagaagga ggcatttttt tactgtcgat gttgacctga atgtctttca
     4021 gcgccaggtc attaatacaa acgctactgt tccacaggca ctcaagaccg accgccagat
     4081 gcagattacc cgcttttacc gccacgcctg gctgctcata acgaacgtca gacaaggtga
     4141 gatcgcgcca gccgccggtg accttgccaa tatccagtcc tggcacccag cgatccgccg
     4201 ctttaaatac aagatgcagg ccgctggtgg tgcccaccag aaacgccacc gatcccagca
     4261 acagtaagat aacgataacc acgccgaggc tgattttttt ccataaactc ataattctgg
     4321 ccccagaccg atgtaaaact gtaacccgtg ttcgtcttta tccgcgaccg gtacggcaaa
     4381 atcgagtttg attggcccga ccggcgattc ccagcgcacg ccgaccccgg taccggtttt
     4441 aaagtcgctg cggcgaatat cgcttaccgc ttcgccacta tcgacaaaca ccgcgcccca
     4501 ccattttccg gtcacgttgt actggtattc cagcgatccg gttatcaact tcgaggcccc
     4561 tttcaggtca ccgttggcgt atttcggggc gatagatttg tatttgtagc cgcgaatact
     4621 gcgatcgccc ccggcgaaaa aacgcagatc cggcggtact ttgtcgaaat caccggtttc
     4681 aatccagccc agcgtgccgc gtgtaacgaa acgatggcga tcgtacagtg tgcggatcca
     4741 gacgttctgc gcctggaaaa cggagaaatc gacatctgag ccccaggccg tgttggagta
     4801 gtcgatagag tagcgttgcg agtcgcccca ggttggcatc aggccaccac aagaacgcgt
     4861 gcggctaatc atcaccccag gataaaacag catcgtggtg ttggtaattt caccctgagt
     4921 aaagtggtcg agactccagc gcaggttaat ggcacgctgc caaccgctgg agagatccca
     4981 gtagcgagaa gccaccagcg tagtggagtc agattcggta tcgttcaggt cagtgcgctt
     5041 aaaaccgccc tgcaccaaat aatattgttc cagtggattc ttcagcagcg gcattttata
     5101 gctgaagtcg agggtctgtt ccggcgcgga aatactggta ctggtggtca aactgtgacc
     5161 gtatgagttc atccacggct ttttccacgt cgctttcacg cgcggtccca cgtccgtaga
     5221 gtaaccgacc ccggtttcga tggtgttttc tgttcgcggc gaaaccacgc ccgtcaatgg
     5281 taatactttc gtttcgcgcg ctttatcaaa ttgtggagcc accaccaccg agttaaacca
     5341 gccggtagca gaaagtcggc ggttcagttc cgccagatct ttcgattcat actcatcgcc
     5401 ctctttaaac ggcaccagat tttgcaggta ttcatcgcgg atttgtgatc cttcaaaggt
     5461 cacatgccca aagcggtaac gttcgccact gttataatca atatcccaga aggctttatg
     5521 caggccgagc gcaatgccca gctgcgcttt ggtaaattcg ctatcgaaat aacctttacg
     5581 caacgcaata ctggttaagg actttttgaa attttcataa tcgccctggt tcagtaccgt
     5641 accaatagcc gggcgagtat cgagcaattt caaatagtct ttatcggtcc gcgcgccgcc
     5701 gcgcaatacc acatcggtgc cgccaattaa caccggcaca cctggcgtga ctttggcgat
     5761 caatacctgc cgccctttct ttggcggtgg acggagatca aattcaatgg tcggctggta
     5821 ataacccagc gctttcagac cttcacggat ggcatcatcg acgcgtgcgc gaaagcggcg
     5881 gtctggcgtc acttcatcac tttcaatcgt agaaagctgc gcacgaacgt tcttttccag
     5941 ctgtcccgat aacccctcga cctgtagacg gacgttcgcg gcgacggcag atccgcttaa
     6001 gcagagtaag cttacacagc ataactgtcg gatatagcgc acattttctc ctgaatatcc
     6061 ttttttcctg cccctggaac gccgttaaac ggcttaacaa aaatccagta atatggatta
     6121 aaaagcagac taaaccccaa atatttctta tgttttactt tagacctatt cacggtggtt
     6181 attgtgtgca aatacgcctc ttgttacaac cttaacccca atgaccgatt ttcgggagag
     6241 cgacaccatg agtttatttg ataaaaagca tctggtttcc cccgccgatg ccctgcctgg
     6301 acgtaacacc ccgatgcccg tagccacgct gcatgcggtc aacggtcact caatgaccaa
     6361 tgaacctgac ggaatggaga ttgccatttt tgcgatgggt tgtttctggg gtgtggagcg
     6421 tctgttctgg cagttacccg gcgtttacag caccgccgca ggctataccg gcggctatac
     6481 gccaaatccg acttatcggg aagtgtgctc cggtgatacg ggtcatgccg aagcggtacg
     6541 cattgtttac gatccttccg tcatcagcta tgagcagttg ctacaggtat tttgggagaa
     6601 tcacgatccc gcccagggaa tgcgtcaggg caatgaccac ggcacgcagt atcgttcagc
     6661 gatttatccg ctgaccccag aacaggatgc cgcagctcgc gccagtctgg aacgttttca
     6721 ggcggcgatg cttgccgccg atgacgatcg tcacatcacc acggaaatcg ctaacgccac
     6781 accgttttat tatgccgaag atgaccacca gcaatatctg cataaaaacc catatggtta
     6841 ctgtggaatt ggcggaattg gcgtctgtct gccgccggaa gcatagcgtt acgggtacaa
     6901 atgtagtttg ttgataaagt gctctttgtc tatgcctgat gcggcttaaa cgacttacca
     6961 ggcctacaaa atcgtccaaa ttcaatatat tgcaaggact gcgtaggcct gatcggcata
     7021 gcgcatcagg cagtattgca tttatcagca gtctgaattt taaccctctg gcgactttac
     7081 agtaccttac gctatactag ccactgaaaa ttccggttca ctttcttcga atcggctttc
     7141 aatgtgtatt tcacacaaat taatcaactt tcccttccga ggatctggcc tgaaaggtcg
     7201 gataagatat gttaaacagt attttagtca tactctgctt gatcgctgta agtgcgttct
     7261 tctcgatgtc cgagatctcg cttgccgcct cacgcaaaat caaacttaaa ctgctggctg
     7321 atgaaggcaa tataaatgcc caacgcgttc tgaatatgca ggaaaatccc ggcatgttct
     7381 ttaccgtggt ccaaatcggt ctgaacgcag tggcgattct cggcggtatc gtcggtgatg
     7441 cggcattttc tccagctttt cacagcctgt tctcccgcta tatgtcggca gagctctctg
     7501 agcaactgag ctttattctc tctttctcgt tagtgactgg catgtttatc ctgtttgcgg
     7561 atttaacccc gaaacgcatc ggtatgattg cgccagaagc ggtggctttg cgtatcatca
     7621 acccgatgcg cttctgcctg tacgtttgca ccccgctggt gtggttcttc aacggcctgg
     7681 cgaacatgat cttccgtatt ttcaaactgc caatggtacg taaagatgac atcacttctg
     7741 atgacatcta cgcggtagtg gaagccggtg cgctggcggg cgtgttacgt aaacaggaac
     7801 acgagctgat tgaaaacgtc tttgagctgg aatcccgtac cgttccgtct tcaatgacac
     7861 cgcgtgaaaa cgtgatttgg tttgatctcc acgaagatga gcaaagcctg aagaataagg
     7921 tggcggaaca tccgcactct aagttcctcg tctgtaatga agatattgac cacatcatcg
     7981 gttatgtcga ttctaaagac ctgctgaacc gcgtgctggc taaccaaagc ctggcactga
     8041 acagcggcgt acaaattcgc aacacgctga ttgtgccgga tacgttaacc ctttcagagg
     8101 cgttggaaag ttttaaaacc gcaggtgaag acttcgcggt gatcatgaac gagtacgcgc
     8161 tggtggtggg gatcatcacc ctcaacgacg tgatgaccac gctgatgggc gatctggtcg
     8221 gtcaggggct ggaagagcag attgtcgccc gtgatgagaa ctcatggctg attgacggcg
     8281 gcaccccaat tgacgacgtc atgcgcgtgc tggatattga cgagttcccg cagtcgggca
     8341 actacgaaac catcggcggc tttatgatgt ttatgctgcg taagatcccg aaacgcaccg
     8401 attcggtgaa attcgccggc tacaaatttg aagtggtgga tatcgataac taccgcatcg
     8461 accagctgct ggtgacccgg atcgacagca aggccaccgc cctttcgcca aaactgcctg
     8521 acgctaaaga taaagaagaa agcgtcgcgt aacccccaga aacatcaacg gctcctgaat
     8581 caggagccgt cttattactg catagcactt tggttaagcc atctctgttt gcagacgcat
     8641 aacctgacgg ttaacttcgg acatcactga caaatgcagc ttatccttca cttttgggat
     8701 aagaatctta cccttatcaa attcaaaagc gccaacgtcc ttgatgtata accgtccacg
     8761 gaacaggatc tttacgtact tcgccacctg aagtgggttg taccgttgga aaattttcat
     8821 tgttatctcc tgctgagtat tacgcctgtg cggtgccaca atcggcccaa ctattatgag
     8881 acgcaaattt taatgcttag tgactataga ctatccgggc aatgtttcca ccgcgtataa
     8941 ctttttttac ctaaaggtta caattattca gaattatctt tttaccgaag cgcggtcttc
     9001 agtataagca ttcatttttc atatgatttg tgcgcttgac cgcaaactgg catcacactt
     9061 gcgggaaatt cgataaatag cacatatgat taaaactcag atccaagtgg tcggatcacc
     9121 tgcatatcat aagaaggaaa caccatgacc ctacgcaaga ttctggcact cacctgcctg
     9181 ctgttgccga tgatggcttc cgcacatcag ttcgaaaccg gtcagcgagt gccgccgatt
     9241 ggcattaccg atcggggcga gttggtgctt gataaagatc agtttagcta caaaacctgg
     9301 aacagcgcgc agttagtggg aaaagtgcga gtactgcaac atattgctgg tcgcacctct
     9361 gcaaaagaga aaaacgcgac gctgattgaa gcgattaaat cagcaaagtt accgcacgat
     9421 cgttaccaga ccaccaccat tgttaacacc gacgacgcaa ttccgggttc cggcatgttt
     9481 gtgcgcagca gtctggagag taataaaaag ctttatccct ggtcgcagtt tattgtcgat
     9541 agcaatggcg tcgcgcgcgg tgcctggcag ctggatgaag aaagttccgc tgtagtggtg
     9601 ctggataagg atggtcgcgt gcaatgggcc aaagacgggg cgctcacaca ggaagaggtg
     9661 cagcaagtga tggacctgct gcaaaagttg ctcaaataat ggcaataaca taaattctgc
     9721 aaaaataatt ttcccctatc gttatctatg ggatagggga attaggtctc tgagctctta
     9781 caccgaactc atactggtaa cgctagttgc tcttttttaa acgcctcaat ggttgattta
     9841 tatttctcgc aaatctcctc ttcggtaata tcactatcta cggccagatt cgcacttact
     9901 atcgcatcca cttgttcggc taattcgact tgttggcaac ctgcattaac cagaaaatta
     9961 taaataagag agtaatcttc atcctccaaa acaacagagg aaccattacg aaaacgttca
    10021 tcccctccat agattttaag cgcaaagccc aaagattcta tgactatggc aaaattatca
    10081 tcaattattt tttcgtaaat ataccatcca tcgcattctt taattccacc tcctggtatt
    10141 tggagttcaa atttaccccg ttttaatttg cttactatat cctccaataa ggcataacct
    10201 aaagacacgt caccaatcgc ctggtgaata taacctttaa tccttttcgg tatgtcagac
    10261 tgttttttct gcagaatatc ccaagcatac aatcttctgt tttgttctaa cggaaattca
    10321 cggtttttga aatccatgaa tgagttatac tcatttattg ttatggggat ttcttcgtat
    10381 tcaaatccac tgcctcgcaa tgtcattgta tattcggcac cgttatcttt aaggctgaaa
    10441 ctgataagaa acttatccat ggctttaaca gtttcatttt ttgatgaaag cacttgataa
    10501 ctatctttat caatctgagt tgcgcaacga aaaaactccc acaaagttaa caggctatca
    10561 taattcgaaa cccaatcttt tttcttaaat gcatgctgaa tattattaaa aaaattgttt
    10621 gaatgtgttt ttgtatgcga tgattttacg aataactcca ccgcaccaat aaaattattg
    10681 tgtctgatcc tgggtaacat atggctacct tattttattt ggttatttta tgtcgccatt
    10741 aaatatctca ttatagaagt aaactatcaa aaaacgctca ttattaaaat gtaacttatt
    10801 agcaattgaa tttttgcact ttaaatacaa tctcttttta tttataactt tttaggttgg
    10861 gagaaatcag gatggcagaa tcaggaaata cactgtttct gccatctgaa tttagtaaat
    10921 agacactctg aacccaggat tcaggaacga ctcgcgcgga gtgtaatcca gcggtttacc
    10981 ctgccagtcg tgaacgtgcg ctccggcagc tgcggccaca gcatgtccag cggcggtgtc
    11041 ccaaatattc gttggtccga agcgcgggta cagctgcgcc tgtccttccg ccaccaggca
    11101 gaatttcagc gaagagccga tggacgtggt ctgatgttcg ccaagctgtt gcagatactc
    11161 tttcagctcc gcatctgcat gggaacggct gataaccacc agcggcgggc gcgcatcgcg
    11221 gacctgaatc tgcttgcgca caccgcactc ttctttccag gccttgcctt ctgccgcgct
    11281 gtacattacg ttcattaccg gcgcatacac cacacctaaa atcggtttgc catggtcaat
    11341 gagcgcaatg ttaacggtga attcgccatt gcgtttaata aactctttag taccatccag
    11401 cgggtctacc agccagtaac gctgccagtg ctgacggact tcccaaccgg gaggatcttc
    11461 ttcagaaagg accggaatat ccggtgtcag cgtacgtaaa ccgtccatga taacggtgtg
    11521 tgcggcaata tccgctgccg ttaccggaga attgtccgct ttgctgacga cgtccatcgg
    11581 tttcgtcccg tcgtagacct gcataatggc atcgcctgca ttccgtgcaa gctggcatac
    11641 ttgatctaac atttctccac ctcgtctctg tgagcggtgt taacttattg ttttacttat
    11701 accctatcgt taatgaatgc gccaactgtg atagtgtcat cattttcaaa gcgtaaaatt
    11761 gtggcattct tcactgttct ataagtaaga cgtttattct cccttttctt tcgtattccc
    11821 gatgataaaa ggatgtccct gatgattaag tttagcgcaa cgctcctggc cacgctgatt
    11881 gccgccagtg tgaatgcagc gacggtcgat ctgcgtatca tggaaaccac tgatctgcat
    11941 agcaacatga tggatttcga ttattacaaa gacaccgcca cggaaaaatt cggactggta
    12001 cgtacggcaa gcctgattaa cgatgcccgc aatgaagtga aaaacagcgt actggtcgat
    12061 aacggcgatt tgattcaggg gagtccgctg gccgattaca tgtcggcgaa aggattaaaa
    12121 gcaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac ggtcggtaca
    12181 ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct ggcgggagcg
    12241 aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc aatgtttaca
    12301 ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca gacgctgaag
    12361 attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc taatttatcc
    12421 ggaaaagtga cggtgaatga tattaccgaa accgtgcgca aatacgtgcc tgaaatgcgc
    12481 gagaaaggtg ccgatgtcgt tgtcgttctg gcgcattccg ggctgtctgc cgatccgtat
    12541 aaagtaatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt taacgccatt
    12601 atgtttggcc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat cgaagaggct
    12661 gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat gtggggcgat
    12721 catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca ggtgacgcag
    12781 gcgaaagcgg aagcacggcc gatttacgac atcgccaata aaaaatccct cgcggcggaa
    12841 gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca gttcgtcagc
    12901 aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt gcaggacgat
    12961 ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta cattcagggc
    13021 gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa agttggtggt
    13081 cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac cttccgtaat
    13141 gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag cggtaaagag
    13201 gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgatcc taacagcacg
    13261 aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga tgttattgat
    13321 ggtgtgaatt atcagattga tgttacccaa cccgcccgct atgacggcga gtgccagatg
    13381 attaatgcca atgcagagcg gattaagaac ctgaccttta atggcaagcc gattgatccg
    13441 aacgctatgt tcctcgttgc caccaataac tatcgcgctt acggcggcaa atttgccggt
    13501 acgggcgaca gacatatcgc ttttgcttca ccggatgaga accgctcggt gctgacagcg
    13561 tggattgctg atgagtcgaa acgtgcggga gaaattcacc cggcggcaga taacaactgg
    13621 cgtttagcac cgatagctgg cgataagaaa ctggatatcc gtttcgaaac ttccccgtca
    13681 gataaagccg cagcgtttat taaagagaaa gggcaatatc cgatgaataa agtcgcgacc
    13741 gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac ttctttttcg
    13801 gtctataaat tatcaaccgc atccggcatt tattggcgga tgcgatgctg gcgcatctta
    13861 tccgccctac cagccatgca ccgtagacca gataagctca gcgcatccgg cagttatgcc
    13921 gcatgttcat cccgcaccgc caacacctcg ggcaaattca actcaatcca gtccgccagt
    13981 gcagcaacct tttcgctcac ctgctcgccc agcggcgtga ggctatattc cacatgcggt
    14041 ggcaccaccg gatacgcgat acggttaaga aaaccatcct gttctaacgc ctgtaacgac
    14101 tgcgcaagca tcttttcgct ccccccgccg attttgcgcc gcaggtcgct aaagcgatga
    14161 gtaccttcgc gtagcgccac cagaatcaac accccccaac ggctggtgac gtgtttcaac
    14221 acctcgcgcg acgggcactg ttccgcaaag agattaccct ctttcagttg ttgcgacagg
    14281 ctaacctgac tcatttcata cttacctttt tgtacgtact tactaaaagt aagtttaggt
    14341 gttagcgtat ttaaacacaa gacaaaacga tggagacttc ccatgatcgc tattactggt
    14401 gccactggcc aacttggtca ctatattatt gaatccttga tgaaaacggt tcctgccagc
    14461 caaatagtgg ctatcgttcg taatccggca aaagcccagg ctctggcagc acaaggcatt
    14521 accgtgcgtc aggctgacta cggcgatgaa gccgcactga catctgcact tcagggagtg
    14581 gaaaaactac tgctgatctc ttccagcgaa gtgggtcaac gtgccccgca gcatcgtaat
    14641 gttattaatg ccgcaaaggc ggctggcgtg aaatttatcg cttataccag cctgctacat
    14701 gcagatacct ccccgctcgg cctcgccgat gagcacattg agacggagaa aatgttggct
    14761 gattctggca tcgtttacac cctgctgcgc aacggctggt acagcgaaaa ctacctcgcc
    14821 agcgccccgg cagcactgga acacggcgta tttatcggtg cggcgggcga tggcaaaatc
    14881 gcctcagcaa cgcgggcaga ttatgcggca gctgcggcac gcgtgattag cgaagccggt
    14941 cacgaaggca aggtttacga actggcgggc gatagtgcct ggacgttgac acagttagcg
    15001 gcagagctga ccaaacagag cggcaaacag gttacctatc aaaatctgag cgaagccgat
    15061 ttcgccgcgg cactgaaaag cgtcggactg cccgacggac tggcggatat gctggcggat
    15121 tctgacgttg gcgcatcgaa aggtggtctg tttgataaca gcaaaacgct tagcaaattg
    15181 attggccgcc caacgacaac gttagccgaa agcgtaagcc atctttttaa tgttaataac
    15241 tagttaatta aagtggcatc ctcccgcatc ctctctaata atgacgggat gccgggagca
    15301 atcatgtctg cttcctgaac tttcttctga cagaccaatg gatgccagta atgattagcg
    15361 gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc gggccgttga
    15421 tcgtaccgga atacccggcg atgttgcagt cgatggggcg ttatctggcg ttagggttaa
    15481 ttgcgctgcc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg cgtcgcgact
    15541 ggcttaccgc cttgatgctc actatgatgg gcaacctcat ctattacttc tgccttgcca
    15601 gtgccattca acgtactggt gcgcctgttt ccacgatgat tatcggcacc ctgccggtgg
    15661 tgattcccgt ttttgccaat ctgctttata gccagcgcga cggcaaactc gcgtggggaa
    15721 aactcgcccc ggcactggtt tgtattggca tcggcctggc gtgtgtgaat attgctgagt
    15781 taaaccacgg actccccgat tttgactggg cacgttatac ctcaggcatc gtgctagcgt
    15841 tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg ctgcgggaga
    15901 atcccgacaa acatccgatg atgtgggcga cggcgcaggc gctggtcaca ctgccggttt
    15961 ctctcatcgg ctatctcgtc gcctgttact ggctgaatac gcaaacgccg gacttctccc
    16021 taccttttgg cccccgtccg ctggtgttta ttagtctgat ggttgcgata gccgtgcttt
    16081 gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg acagtgattc
    16141 tcgggccgct gatcgttttc gaaacactgg caggtttgct gtacaccttt ttgatacgcc
    16201 agcaaatgcc gccgctgatg acgctgagcg gtatcgcgct gttagtggtt ggcgtggtca
    16261 ttgtagtcag agcaaaaccg gaaaagcctt taactgaatc tgtctcagaa agttgacata
    16321 ctggctgtga gttaaataag cctctgctac gtaagggtta tagcttttgc cttaaagatg
    16381 tatttaaaat acatcttatc ttattaagaa tgaggtatca gctatggctt atcgcgacca
    16441 acctttaggt gaactggcgc tctctattcc tcgcgcttca gctctgtttc gtaaatataa
    16501 tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac gtaaagaact
    16561 ggatgttgag gtcattgaag ctgaactggc aaagcttgct gaacaaccga ttgagaaaga
    16621 ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct accacgatcg
    16681 tcaccgcgag caactgccgg agctgatcct gcaagcgact aaagtcgagc gcgttcacgc
    16741 cgacaaaccg agcgtgccaa aagggctgac aaaatacctg accatgctgc atgaagagct
    16801 ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac aaggcatggg
    16861 cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag cgggcgaact
    16921 gctggaagtg atcaaacaca ctaccaataa cgtcacaccg ccgccagaag cctgcaccac
    16981 ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg atcacatcag
    17041 tctggaaaac aatgtactgt tcccacgcgc gctggcgggt gagtgatcta aaagctggat
    17101 ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag cccgcttctt
    17161 accaataaac aaccagccca gccccagtgc gataaaccac agcggggtaa ccagcagcgc
    17221 ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga acgccataca
    17281 tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttctcat gcagatgagg
    17341 acgctgtttg cggtacacca ggtacgagca aaggataatc gtccagacga acataaacag
    17401 aatcgcggaa acggttgtaa tcatcgtgaa cgcaccaatc acgctaggat tcacatacag
    17461 catcaccacg ccaccgagca ggcagatgca ggagaaggtc agccctttcg ctggtactgc
    17521 gcgcttagaa agtttagcga acgctttcgg tgccacacct tcctgcgcca gaccaaacag
    17581 catacggctg gtagagaaga cgccgctgtt agcggaagac gccgcagagg tcagcaccac
    17641 aaagttgatc acgctggcag ccgcaggcaa acctaccaac acaaacagct caacgaacgg
    17701 gcttttctcc gggactaccg aactccacgg tgtcacggac ataatcacaa tcagcgcgaa
    17761 gacgtagaac ataatgatac ggatcggaat ggagttaatc gcgcgtggca gtgatttctc
    17821 cggatctttg gtttccgcag ctgttgtacc taccagctca atccccacga aagcgaaaac
    17881 cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc cgccgtcatt
    17941 ccacaaatgc gcgaatgacg cttccacacc agtcggtgac tgaaagtgca tcgccaccat
    18001 gaccaggccg acgacaatca gcgacacgat ggcgacgatt ttgatcatcg caaaccagaa
    18061 ctccatctca ccgaacattt tcacggtggc aaggttgagc gtcagcagca gcactatcac
    18121 cgccagcgag gcgacccagt cggagagatc ggggaaccag aactgagcat aagccgtgat
    18181 cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc agccagtgaa
    18241 atatcctgtc cacggcccga gtaaatcgga agcgaagtca ctaaaagatt tgtattccag
    18301 attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa aaccaatgat
    18361 catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccag accccagaaa
    18421 caaccccgta ccaatggcac cgccaatggc aataagctga atatgtcggt ttgtgagatt
    18481 gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta cctgatctac
    18541 catgtttttt tcttcctgta cctgtctgtg ttgttcaggc tctacggcct ttagtcagtc
    18601 tatgatacga cgaatctgta tcgggactca tcgatattag gtaacaatac gcgggatgaa
    18661 tactaagatt tagatctaat gttaatttta tgtttaaagt gagtgtcata tcacggtgat
    18721 aacgcgaaac atctcacaaa aatacacgct tacggggtct ttgcaacaca aaatagcgcc
    18781 gtttggcaaa ctgaagggtt tattgctgaa tgcctgctcc cctctggttc gaggggaaca
    18841 gaatgcgctg cttagaggat ttccagcagt tcgacttcaa acaccagggt gctgaacgga
    18901 gggatggatg cacctgcgcc gcgctcgcca tatgccagtt cctgcgggat agtcagttcc
    18961 catttagaac ctaccggcat cagagtcagt gcttcaatcc agccagggat cacgccgtta
    19021 accgggaatt cagcgggttc accacgagca acggagctgt caaacacggt gccgtcgatc
    19081 agtttaccgg tgtaatgaac acgaacgcgg tcggtacgtg ccggaattgc gccttcaccc
    19141 tggttgatca cgcggaattg caggccagat tcggtgctat tcacaccttc ttttttggcg
    19201 ttttcttcca ggtatttcac accttcagca gccatcgcct ggaaacgctg acgacgaacg
    19261 gcatcggcgc gttcgtggat ttcacgcagc gcgcgatgca ccacatcaac cggaacagcc
    19321 ggatgtttgc c
//
LOCUS       DAJVTY010000073        19057 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVTY010000073 DAJVTY010000000
VERSION     DAJVTY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19057
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.073"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..732
                     /gene="rihB"
                     /locus_tag="OQQ66_002243"
     CDS             <1..732
                     /gene="rihB"
                     /locus_tag="OQQ66_002243"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1905162.1"
                     /translation="IMRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMA
                     SDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPE
                     AARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFEN
                     YGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTK
                     VGITIDTDWFWGLVEECVRGYIKTH"
     gene            832..2082
                     /locus_tag="OQQ66_002244"
     CDS             832..2082
                     /locus_tag="OQQ66_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1905163.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRVPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2138..3226)
                     /locus_tag="OQQ66_002245"
     CDS             complement(2138..3226)
                     /locus_tag="OQQ66_002245"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1905164.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3229..4086)
                     /gene="nfo"
                     /locus_tag="OQQ66_002246"
     CDS             complement(3229..4086)
                     /gene="nfo"
                     /locus_tag="OQQ66_002246"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1905165.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4160..5209)
                     /locus_tag="OQQ66_002247"
     CDS             complement(4160..5209)
                     /locus_tag="OQQ66_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1905166.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5309..6190
                     /locus_tag="OQQ66_002248"
     CDS             5309..6190
                     /locus_tag="OQQ66_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1905167.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6395..7864
                     /gene="lysP"
                     /locus_tag="OQQ66_002249"
     CDS             6395..7864
                     /gene="lysP"
                     /locus_tag="OQQ66_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1905168.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8157..10137
                     /gene="cirA"
                     /locus_tag="OQQ66_002250"
                     /pseudo
     CDS             8157..10137
                     /gene="cirA"
                     /locus_tag="OQQ66_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10169..11005)
                     /gene="yeiG"
                     /locus_tag="OQQ66_002251"
     CDS             complement(10169..11005)
                     /gene="yeiG"
                     /locus_tag="OQQ66_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1905169.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11263..11931
                     /gene="folE"
                     /locus_tag="OQQ66_002252"
     CDS             11263..11931
                     /gene="folE"
                     /locus_tag="OQQ66_002252"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1905170.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11948..13105
                     /locus_tag="OQQ66_002253"
     CDS             11948..13105
                     /locus_tag="OQQ66_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1905171.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNRAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMCS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13247..14287
                     /gene="galS"
                     /locus_tag="OQQ66_002254"
     CDS             13247..14287
                     /gene="galS"
                     /locus_tag="OQQ66_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1905172.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14567..15565
                     /gene="mglB"
                     /locus_tag="OQQ66_002255"
     CDS             14567..15565
                     /gene="mglB"
                     /locus_tag="OQQ66_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1905173.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15626..17146
                     /gene="mglA"
                     /locus_tag="OQQ66_002256"
     CDS             15626..17146
                     /gene="mglA"
                     /locus_tag="OQQ66_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1905174.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLRRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17162..18172
                     /gene="mglC"
                     /locus_tag="OQQ66_002257"
     CDS             17162..18172
                     /gene="mglC"
                     /locus_tag="OQQ66_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1905175.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18415..>19057)
                     /gene="preA"
                     /locus_tag="OQQ66_002258"
     CDS             complement(18415..>19057)
                     /gene="preA"
                     /locus_tag="OQQ66_002258"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1905176.1"
                     /translation="AINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALRFI
                     QQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGNRIVEDMASGL
                     SHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGG
                     HQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL"
BASE COUNT         4549 a         4680 c         5067 g         4761 t
ORIGIN      
        1 attatgcgta aacaaatcgt tgccgataat attcacggtg aaaccggact ggatggtccg
       61 gtattcgagt cgctgacccg ccaggcagaa agtacccacg cggtgaaata catcattgat
      121 accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct ttcaaatatc
      181 gcggtggcaa tgcgtatgca acccgcgatc ctgcccaaaa tccgtgaaat tgtgctaatg
      241 ggcggcgctt acggtacagg caacttcacg ccatctgccg agttcaacat ctttgctgat
      301 ccggaagcag cccgcgtggt gttcacctcc ggcgttccat tagtgatgat gggcctcgat
      361 ctcaccaacc agactgtttg caccccggac gtgattgcac ggatggaaag ggcaggcggc
      421 cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaaaac
      481 tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct gattaaccct
      541 gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg cccttgctat
      601 gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc ccgctaatac caaagtcggc
      661 atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc
      721 aaaacccatt aagtgttgat aacaagccgg gtcagtatcg caagatccaa gcccggctat
      781 ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataataac tatggatgtc
      841 atgagaagtg ttctgggaat ggtggtattg ctgacgattg cgtttttgct gtcagtaaac
      901 aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc
      961 gggattatgc tttggttacc ggcagggcgt tgggtcgctg aaaaagtcgc ttttggcgtg
     1021 cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc gctggtcggg
     1081 ccgaaaatgg acacgctgtt tgatggcgca ggatttatct ttggtttcag ggtattaccg
     1141 gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatt
     1201 ttaattcgca ttctcggcgg tatattccag aaagcattaa atatcagcaa gattgagtca
     1261 ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc
     1321 tttatcgatc atctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg
     1381 attgctggtt cgacaatgat tggttacgcc gccctgggcg tacctgtgga atatttgctg
     1441 gcggcatcgt taatggcgat cccaggcggg atcttgtttg ctcgcctgtt aagcccggct
     1501 acggaatctt cgcaggtttc ctttaataac ctctctttca ccgaaacacc gccaaaaagc
     1561 attattgaag ccgctgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg
     1621 acagttgtta tggcatttgt cgccatcatt gcgttaatta atggtattat cggcggcgtt
     1681 ggcggctggt ttggttttgc acatgcctcg ctggagtcca ttttaggtta cctgttggcc
     1741 ccattggcgt gggtgatggg ggttgactgg agtgatgcaa atcttgccgg gagtttgatt
     1801 gggcagaagc tggcgatcaa tgaatttgtc gcttatctca atttctcgcc atatctgcaa
     1861 acgggtggca ctctggatgc taaaaccgtg gcgattattt ctttcgcgtt gtgcggtttc
     1921 gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt
     1981 gtgccggaaa tcgcccaact tggtttacgc gcgctggcgg cggcgacact ttctaacctg
     2041 atgagtgcta ctattgcagg attctttatt ggtttagcgt agtgcgcaat ttatgagccg
     2101 gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaaa
     2161 gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgc gaaattgccg
     2221 cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta tccttcaaga aagctatata
     2281 ccaggcccgc cataaaacca tcgccagcac caaaactgtc tactgtcgta tgcgctggcg
     2341 cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca tcgggcaaat
     2401 aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag
     2461 catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg
     2521 tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga
     2581 caaacaccgg gatttcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt
     2641 tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt
     2701 gcggtgtcaa ctgttccagc agatgggtat cgttaatcgc cagcacggtt tgatcgtctc
     2761 gattggcaat tgccagatac gtcgatgtgc tttgaccatg caaacgaacg cagccggaga
     2821 cattcacgcc ggcacggcgc gtttcttcca ggagcatttc gccataaaag tcatcgccaa
     2881 tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg atgttgcgtc
     2941 ccacgccgcc cgccgagcaa tgaattgtac cgggatgaga agccgcttgc gggtaacgga
     3001 tatccgcgat cccgcgaata tccatattga ttgtccccac cactacgcag tattcctgct
     3061 cggtgagaat gtaacctttg cctttaatcc gccctttgcg cattaaatcc ataatatgcg
     3121 ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat ttcgttctgc tgaatcagcg
     3181 ggttacgccg taaaattgca aggatctcct tttcccggtt attcatcttc aggctaccgc
     3241 tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat
     3301 ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc agcggaacgc
     3361 atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt
     3421 gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccaacaa tacgggcaaa
     3481 atccgcgaac gttttctcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc
     3541 atggcaggta tcaatgcaga cgccgacacg ggatttatct tccacgccgt cgataatcgc
     3601 cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt tttcaatcac
     3661 cgctgttaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag
     3721 gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag
     3781 ccccagttgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc
     3841 ttcagtgacc ggatgtccga ggttaatcag atagctgtcg tggggaagaa tttgcgccga
     3901 tgtgtagtga tatttttcac aggctgcttt gaattcatcg atggtttgcg tcgtgagcgg
     3961 tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc
     4021 ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta
     4081 tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac
     4141 aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac
     4201 caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc gccccggctt
     4261 ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga
     4321 aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa
     4381 agatggcaac gacgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc
     4441 cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca
     4501 gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc gggctgatgg
     4561 catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga
     4621 aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatcg
     4681 cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt
     4741 tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga
     4801 tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca
     4861 ggaaggtact ggacagcgtc aacacgtcaa tgatgatccc actgataccg acatcggcaa
     4921 tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct
     4981 tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta
     5041 aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg
     5101 gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa
     5161 taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct
     5221 tataaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt atatatttat
     5281 aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt tggaagtttt
     5341 tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca
     5401 atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc aactgtttga
     5461 tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc cgcgtgcgtt
     5521 ggcattgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca acggcgcgat
     5581 tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta tcgcccgtta
     5641 tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc aggacgtgat
     5701 gcaagcggtg ctggatttcc gcgttgatat tggctttatt gaaggaccgt gccacagcac
     5761 tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg cgccgacttc
     5821 gccgttggct cgtggtccgg tcaccttaga acagctggcc gctgcgccgt ggatcctgcg
     5881 tgaacgcggt tccggcacgc gggagattgt cgattatcta ttgctgtcac atttaccgaa
     5941 gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg
     6001 gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg caggcacatt
     6061 aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg
     6121 gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc ccgcgaatat
     6181 accgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc
     6241 cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc cagggcgatc
     6301 atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt ctgctacaat
     6361 tgcgcctcat ttttaagatg gatagcattt ttgtatggtt tccgaaacta aaaccacaga
     6421 agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg ccattggcgg
     6481 ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg caggtccggg
     6541 cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga tgaccagtct
     6601 cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg gtcagaacta
     6661 tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact gggcggtgac
     6721 tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc cggatacacc
     6781 gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact acatctcagt
     6841 tcgtggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga cagttattgt
     6901 ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc agcctgcggg
     6961 ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg
     7021 cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta ttgctgcagg
     7081 cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt tctggcgaat
     7141 cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt acaccgatcc
     7201 gagcctgctg cgtaacgatg ttaaagacat cagcgttagt ccgttcaccc tggtgttcca
     7261 gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga cggcggtgct
     7321 gtcagccggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga
     7381 cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc gtaatgcgct
     7441 gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg gcaaccagac
     7501 ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct ggctggggat
     7561 tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg acattaacga
     7621 tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca ttctgtgtct
     7681 gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg actggggcgg
     7741 cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg gctacaagct
     7801 gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga acgataagaa
     7861 ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
     7921 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg ttatcgtttg
     7981 cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc cggctttctg
     8041 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtga
     8101 agaaggtgtg agcgataacc cattttattt tcgtagttac ctcatggaga tatggaatgt
     8161 ttaggttgaa ccctttcgta cgggtcaggc tgtgtttgtc cgctatttct tgtgcatggc
     8221 ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct tccgttgaac
     8281 aaaacctcaa agatgcaccc gccagtatca gcgtcattac ccaggaagac ctgcagcgaa
     8341 aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg
     8401 aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac accctgattc
     8461 tcgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat gatttcgatc
     8521 tgaactggat cccggtcgat tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt
     8581 cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc
     8641 agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc gatcgcggtg
     8701 atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg ctgggaatga
     8761 aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg
     8821 ataccggaga aatgccgcgt attgaaggat tctccagccg cgacggcaat gtcgaatttg
     8881 cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac cgtcaggaac
     8941 tcgctggaca aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg
     9001 gattacggca ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc
     9061 aacagcagcc cgataacttc cgaaagcaat acggtcgacg gcaaatacac gttgccgctg
     9121 acggcgatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa acttagcgat
     9181 gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt
     9241 gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac
     9301 gatcacgaaa cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc
     9361 gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa
     9421 cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg
     9481 gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa
     9541 ggctggctgg aagggggtga atccagcgtt accgttttcc gtaacgatgt gaaagatcgt
     9601 atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt
     9661 tttgagacgg gcgctaacgg acggcgcata ccggtattta gctactacaa cgttaacaaa
     9721 gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg
     9781 tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta
     9841 tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa
     9901 gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg
     9961 gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa
    10021 gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac
    10081 tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga
    10141 gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca
    10201 ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac
    10261 gcagcgtcat cggccacgct ttctggcgcg cggcttctgc cagtaccgca ggttgcaact
    10321 ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg
    10381 cgttactggc atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac
    10441 ctaaatagct gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg
    10501 caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt
    10561 gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa
    10621 ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg
    10681 gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg
    10741 caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg
    10801 ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa
    10861 gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg
    10921 tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac
    10981 agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc
    11041 atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg
    11101 ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg
    11161 attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc
    11221 ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa
    11281 gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg
    11341 cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa
    11401 atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc
    11461 atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa
    11521 atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact
    11581 ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat
    11641 atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc
    11701 cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg
    11761 ctgggcacca ataacgtggc tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt
    11821 ggcatccgcg atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc
    11881 agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc
    11941 aggaaccatg gagcgcaacg taacgctcga ttttgttcgc ggcgtcgcca ttctggggat
    12001 cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca atcgcgcctg
    12061 gtacggcgct attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca
    12121 ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc
    12181 ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gttttattca
    12241 cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg
    12301 ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaatacag gcgtcatgct
    12361 ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag
    12421 ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg
    12481 cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg
    12541 cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc tgatgtgcag
    12601 cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt
    12661 ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc
    12721 gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc
    12781 gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct ttaagctggt
    12841 gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat
    12901 ttgtaccatg cttttttacc acctcggttt gtttatgcat tttgaccgcc tggagctgct
    12961 ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta
    13021 cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggaac
    13081 ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta
    13141 accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc
    13201 cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg
    13261 tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag
    13321 cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta
    13381 tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt
    13441 ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc
    13501 tcagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
    13561 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc
    13621 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
    13681 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
    13741 aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag
    13801 tcacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
    13861 ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga
    13921 ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctatat ttgcttataa
    13981 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
    14041 acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
    14101 aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac tggccttgca
    14161 gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
    14221 tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc
    14281 gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg
    14341 attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt
    14401 aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg
    14461 aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact gccctgccga
    14521 gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt
    14581 gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga
    14641 tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa
    14701 ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca
    14761 gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc
    14821 actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg
    14881 gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta
    14941 cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc agggcgattt
    15001 gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca
    15061 gtccgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt
    15121 gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat
    15181 gtgggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa
    15241 caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact
    15301 gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct
    15361 ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc
    15421 gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac
    15481 caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa
    15541 cctggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat
    15601 tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata
    15661 cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt
    15721 taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa
    15781 atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt
    15841 ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat
    15901 ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctgag
    15961 gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc
    16021 gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt
    16081 ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat
    16141 tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat
    16201 tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat
    16261 cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc
    16321 gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca
    16381 gcgttttcct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac
    16441 gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct
    16501 cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat
    16561 tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc
    16621 caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat
    16681 ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa
    16741 taaagttggt ttactggata aatcgcggat gaaaagcgat acccagtggg tgattgattc
    16801 gatgcgggta aaaacaccgg ggcatcggac gcaaattggt tcgctctcag gtggtaatca
    16861 gcaaaaggtg attattgggc gctggctact aacgcaacca gaaatattaa tgctcgatga
    16921 accgacgcgc ggtattgatg tcggggcgaa gtttgagatc tatcagttaa ttgccgaact
    16981 ggcgaagaaa ggcaaaggga ttattattat ctcctctgaa atgcctgagt tgttagggat
    17041 aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg acacaaaaac
    17101 aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc
    17161 catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt
    17221 cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt
    17281 gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc
    17341 agggttaatt gtcacccagg ggaccgacct ttctgcgggg cgtcaagtag ggctggcagc
    17401 agtggtggca gcgacattat tgcagtccat ggataacgcc aataaagtgt tcccggaaat
    17461 ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg
    17521 tttgatcaac ggtctgatta tcgcttatct caacgttacg ccgttcatta ccacgctcgg
    17581 cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg gggcgtcgcc
    17641 aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag
    17701 tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt
    17761 gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc
    17821 aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt
    17881 gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct
    17941 cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag
    18001 cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa
    18061 ctatggcctg acgtatatcg gcgtaaaccc atgctggcag tacatcatca aaggggcgat
    18121 tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaaat gatgtgaatg
    18181 tggtccgcat tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg
    18241 ataaggcgtt cacgccgcat ccggcaacag gagccaaatg ccggatgcaa cgctggcgcg
    18301 ttttatccgg cctacaataa gctcaacata ctgatttgta tggttttgta ggccggataa
    18361 ggcgttcaag ccgcatccgg cgacaggaac caaatgccgg atgtgacgcg ggttctacaa
    18421 cgttaccggg tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc
    18481 caccgggcag acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg
    18541 tgtgcgggtt ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata
    18601 acagcgtcca cagccaacac atttgtcaag attgatacgg ggatagacaa tataactgcg
    18661 gtctaaatct tccgccggga caatattgtt attcgccaga cctaccatct cctgcaacga
    18721 atcaaatccc tgatcggcga gataatgcga taacccgctc gccatatctt ctacgatgcg
    18781 attgccgtac tgcataatgc cggtggtgac ctgtaacgtt gctgcgccga gcaataaaaa
    18841 ctccgccgca tcctcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag
    18901 ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc
    18961 ggaatatccg gaaatactcg atttaccgtt aacgatcggc ataccgattt tctgattaag
    19021 atcgatattg gtgatggatt taacggtgtt aattgcc
//
LOCUS       DAJVTY010000074        18887 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVTY010000074 DAJVTY010000000
VERSION     DAJVTY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18887
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.074"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OQQ66_002259"
     CDS             complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OQQ66_002259"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1905177.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OQQ66_002260"
     CDS             complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OQQ66_002260"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1905178.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OQQ66_002261"
     CDS             complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OQQ66_002261"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1905179.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OQQ66_002262"
     CDS             complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OQQ66_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1905180.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWDSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OQQ66_002263"
     CDS             complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OQQ66_002263"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1905181.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OQQ66_002264"
     CDS             complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OQQ66_002264"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1905182.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10958..11551
                     /gene="yhgN"
                     /locus_tag="OQQ66_002265"
     CDS             10958..11551
                     /gene="yhgN"
                     /locus_tag="OQQ66_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1905183.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OQQ66_002266"
     CDS             complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OQQ66_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1905184.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OQQ66_002267"
     CDS             complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OQQ66_002267"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1905185.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OQQ66_002268"
     CDS             complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OQQ66_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1905186.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OQQ66_002269"
     CDS             complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OQQ66_002269"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1905187.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15655..16692)
                     /locus_tag="OQQ66_002270"
     CDS             complement(15655..16692)
                     /locus_tag="OQQ66_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1905188.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17024..17512
                     /gene="yhhY"
                     /locus_tag="OQQ66_002271"
     CDS             17024..17512
                     /gene="yhhY"
                     /locus_tag="OQQ66_002271"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1905189.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17749..>18887
                     /locus_tag="OQQ66_002272"
     CDS             17749..>18887
                     /locus_tag="OQQ66_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCS1905190.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGS"
BASE COUNT         4824 a         5003 c         4599 g         4461 t
ORIGIN      
        1 ccgttctatt tattggtgaa cttacaatct caccggatcg atatgccaga tatgatcggc
       61 gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
      121 ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct gacaatcgac
      181 atagctgcga taatccgcca gaacctgata gtgatcgccg aagttgatca gcgaatcaac
      241 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
      301 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
      361 cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag caccgacatg
      421 atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac cgttaagcgc
      481 aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct gttcagacag
      541 atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga tgaacacgac
      601 tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
      661 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac
      721 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg taatcacatg
      781 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta catcgaacaa
      841 cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca gacgcttttt
      901 gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac agtgttgttg
      961 cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac
     1021 ggctgaaagc gatgggttcg ctaccgctag ccagcgacgc ggcgtcacac cgttggtgac
     1081 gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt gcaccatcag
     1141 attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg ccagccaggc
     1201 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
     1261 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
     1321 ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt ccagcgcctc
     1381 gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc
     1441 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagtaccgg
     1501 atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa
     1561 ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accagaaagt attcctgacg
     1621 caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc gagatacgtt
     1681 ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccttggttga atttaccgag
     1741 gttaatttcg ctactggctt gcgcactcca caaacgcaac gtgttggtcg cgtcggtgtc
     1801 gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt caatccagcg
     1861 cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc gcgtgttgtg
     1921 gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact ctttctggct
     1981 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
     2041 cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc ccaggccacc
     2101 gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta accccatcgc
     2161 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggagagcgt
     2221 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
     2281 ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
     2341 gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa acatcagctt
     2401 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
     2461 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
     2521 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
     2581 cagtgaagca cgagaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc
     2641 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
     2701 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
     2761 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
     2821 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
     2881 gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
     2941 gagtagcgcc agctgcccgc cctgctccag aagacccggt aaggcttcca gcaccaggtc
     3001 gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
     3061 cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc
     3121 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
     3181 gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
     3241 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga ccgccgtaat
     3301 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
     3361 atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
     3421 gccttgatag gccaagttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc
     3481 cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca
     3541 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
     3601 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
     3661 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
     3721 gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtca cgccacggcg
     3781 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
     3841 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
     3901 acatacatgt aaaacttgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
     3961 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
     4021 ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac gcagcggcgc
     4081 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
     4141 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
     4201 acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
     4261 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
     4321 atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta agctttcagc
     4381 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
     4441 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
     4501 tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag atagtcggcg
     4561 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
     4621 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
     4681 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
     4741 ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
     4801 aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg ggtgaccgca
     4861 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
     4921 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
     4981 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta
     5041 gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
     5101 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
     5161 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
     5221 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag
     5281 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
     5341 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
     5401 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
     5461 atcgatttag ccaacggaca ttgccatcgc cttcttccca ccagcgattc tccaccaaag
     5521 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
     5581 cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat
     5641 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca
     5701 acgttgttaa caaggcgtga atactgtcgc gccgccgttc aaccaggtca agagaaccgc
     5761 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
     5821 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
     5881 catgcgcagt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
     5941 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgtagccaga
     6001 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
     6061 ccacctgata accaccaagg gcgatatccc acggttcagc aattaacttc acctgcgaga
     6121 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
     6181 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtgacaggtt tctacccaat
     6241 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
     6301 cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttat
     6361 cgatcccacg cagtgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
     6421 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
     6481 catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg
     6541 ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca ctggcaaact
     6601 gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag ttgatcatca
     6661 ccggatgccc gagggcttta taagtgccac ggatctcgac cggaatctcc gggtgcaagt
     6721 acgttaatcc tttgacatgg gcttcataaa tgatggtgct gtcccacggc gtgcgcggcg
     6781 gggcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
     6841 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaatt
     6901 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
     6961 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat
     7021 ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct
     7081 ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga
     7141 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca atggcgagtt
     7201 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagtg tcaggcttag
     7261 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgcctgc
     7321 attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt tgcctggctg
     7381 attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg caacgataat
     7441 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
     7501 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt tatggtggcg
     7561 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccacc
     7621 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcacggccct gggcaaattc
     7681 gttccccatg aacagcagtt tcttgcccgg gaatgcccac atccagccat agtaggcgcg
     7741 caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc
     7801 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagaat
     7861 cccgaaggtg agtttatcgt gatgatactg acgataaacc gggtcgagct tcatgtagtc
     7921 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
     7981 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
     8041 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
     8101 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cgcgataaat
     8161 cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
     8221 cgcgttaccg acgaggaagt tactgacttc acggcgacca taattgtaga tcagcgtatt
     8281 ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg tgccatcaaa
     8341 ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggc acccagtcga gaatcacgtt
     8401 cagacccgct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
     8461 gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc
     8521 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
     8581 atcggccagc tcgcggtagc tcaaccagaa attgttatcg gtgtggcgac gccaggagcc
     8641 caggtgaact tcataaatag agattgacgc atcaaactga ttcgcttttt tgcgctcttc
     8701 agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
     8761 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
     8821 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaaa tgccgctctc
     8881 tttacgcaga cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
     8941 gacccgacgg gcgtttggag cccaaacaga gaaacgcgta cctgtgacgc catccatagt
     9001 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
     9061 ggcatccatt tcctggatta gcgggccaaa acggtaagga tcatcaatca ggttttgctg
     9121 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacacc
     9181 gctaaagaat ccccgtgagt caagacactc cagttttgcg agtttgcgcc cggttttcgg
     9241 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
     9301 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
     9361 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
     9421 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
     9481 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
     9541 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
     9601 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
     9661 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
     9721 tcacccacgg taaaggctga caggaactcc ggtcccatat tcagcttacg caggcggcct
     9781 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
     9841 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttcgacggtc
     9901 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
     9961 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    10021 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
    10081 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    10141 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    10201 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    10261 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
    10321 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    10381 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg
    10441 atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat
    10501 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    10561 atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt
    10621 ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac agggcgaatg
    10681 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    10741 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    10801 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag
    10861 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    10921 acagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    10981 agttttattg atcctgatta tggatccgct cggaaacctg cctattttca tgtccgtact
    11041 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11101 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    11161 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca ttaagatgat
    11221 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
    11281 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11341 tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
    11401 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11461 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct
    11521 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agaacccggt
    11581 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac
    11641 gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    11701 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac
    11761 aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac cggagtagtt
    11821 cagttgttca atgaccggca tcaccagccc caccgccgtt aaacaggcaa cggtggcaga
    11881 cccctgaatg atgcgcactg cagcggccag cacgaagcag gtgatagcaa tcggcaggcc
    11941 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    12001 gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgta gcgcgtgacc
    12061 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    12121 taccagacaa gcaaccagaa tcgcggtaaa tggatgacca ataaactcga accattcgta
    12181 agcagttgat ccttctggca caaaacgagc ggcaatggtt ttcagcccca ccagcaccag
    12241 cggcaacagg atcagcgaca ggctgaatcc gaaggacggc attttgcctt cgccgagatg
    12301 cggttcgctg atgtcgtcag gaatatgcaa ctccacgtaa cggctgatga aattacccca
    12361 cagcggcccg gcaataatca ttcccggaat tgccgcacac agaccaatca ggatcatcca
    12421 gccaaagtcg gcattcatct gcgatgccag caacatcggc gctggtccag gcaccagaaa
    12481 tgccgccgct gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    12541 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga actatcgcca cttcaaagaa
    12601 cagcggtagc gcacagacca accccgcaag gccgatggca taatgcgcgc ggctgtgacc
    12661 gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
    12721 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggggc ctcccatccc
    12781 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    12841 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    12901 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    12961 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13021 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13081 catttgggtt ttaaagaaat ggcctttgcg cgctttcaag cgactttcaa tcacatcaaa
    13141 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    13201 gtgctttttc agtgcagaac agacgatcag cgacacttta ttggtgcgca gcatagcaaa
    13261 cgcggcgtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca gcggttcgcc
    13321 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcaccatcaa gaaacgcggc
    13381 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    13441 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13501 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga
    13561 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtaggctta
    13621 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    13681 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    13741 cagcacgctc gctaaccgcg gctccatcac ctggccaatg tcatgaccgt ggaaaccggc
    13801 aatcgccata tcgtcaggaa ccttcaatcc cagacgctga cattcaaacg ccgcgccgac
    13861 cgccaggtca tcgttggtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    13921 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatacga
    13981 caccagacct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    14041 gtcgagacgc gcgccgagat aggcaatgtg gcaatgcccg cgagcaataa tggcggtggt
    14101 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
    14161 gctgtccatg agttccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    14221 cgtgtgggta cgttcggtga ggatcagtcc gtcgatattc catgagagca tggattcgag
    14281 acgttcttgc tccatttccg gtttataacc gtagtgagcc agcatggtct gataaccgtg
    14341 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    14401 aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg gcgcacgatt
    14461 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14521 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    14581 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    14641 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    14701 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    14761 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    14821 aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    14881 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    14941 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
    15001 caatctgatg caccgactgc tcatctttca gcaacgccca gcggtacagt tccatatcct
    15061 gatgcacttt caatgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgta
    15121 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatacc gttttcttca ggcatgatcc
    15181 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    15241 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
    15301 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15361 ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15421 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
    15481 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    15541 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15601 tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttaatt
    15661 cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    15721 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
    15781 cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    15841 gccctcgtca ttgacatact ccggcacacc gaccgaatca tccgctgcga atcccggttc
    15901 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    15961 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16021 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    16081 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16141 aataatctgg tccatcgtat gcacaccaag gccataaaac gcgccatcct gcggcagccc
    16201 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt caacaatctc
    16261 tcccaacttg ccactttcaa tcgctttttt cgctgtcagg aagcaggagt caaagcgacg
    16321 attctgatac ggcgtgacgc tcagcccttt gcttttcgcc aacgcaaaca gctccttcgc
    16381 ctgggcaagc gtcggggtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
    16441 gcgtttcgcg tactcgaaat ggctgtccgc gtgggtgcag acaacaacca gcttaacatc
    16501 gggatcgttt agtacttcgt cgagatcgct gatgaaatgg atatgggaat aaatgggagc
    16561 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
    16621 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    16681 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    16741 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
    16801 gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    16861 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat aataatcatt
    16921 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    16981 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    17041 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    17101 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    17161 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    17221 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    17281 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    17341 gtgcgacaac tggttgcggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcccc
    17401 ggcaattaag gtctataaaa aatacggctt tgaaattgaa gggactggta agaagtacgc
    17461 attgcgtaat ggtgaatatg tcgatgcata ttatatggcg cgggtgaagt aagatagtgc
    17521 cctttttctg agatggaaaa agggtgtcat tcaaaatcga cataccttcc tttaaggtat
    17581 ttatttaccc aatacatata ttgagattta actcataatt acttcctaaa gtgtaatatt
    17641 ttatttttta atatatacgc ctacaatttc ctggagtaaa taaataacaa ttaacaagca
    17701 taatattgcc attgataaaa tagcatgcca taaaaggact tttcagggat gagtaatatt
    17761 gtttacctga cagtaacggg agaacaacaa ggaagcatct ccgcaggttg tgggacttct
    17821 gagtctacag gtaatcgttg gcagagcggg catgaggatg aaatatttac attctcactc
    17881 ttaaataata ttaataatac ggggcttggt tcacagttcc atggtataac attttgtaaa
    17941 ttaattgata aaagcactcc attatttatt aattccatta acaataatga acaattattt
    18001 atgggatttg acttctatcg aataaataga tttggtagat tggaaaagta ttattatata
    18061 caactaagag gcgctttttt atcggctatt catcaccaga tcattgaaaa ccaactggat
    18121 acagaaacaa taactattag ttatgaattt atcctctgtc aacatcttat cgcaaatacc
    18181 gagttcagct atttggcact ccctgaaaat tataaccgat tgtttttacc aaattcaaaa
    18241 aaccaaacaa ataatcgttt caaaacgtta aacagcaaag ctattggcag gctacttgct
    18301 gctggtggcg tatacaatgg gaacattgaa ggattcagag atactgcgga aaaactgggt
    18361 ggagatgcaa taaaaggcta tgatcaaata ctaaatgaaa aaacagcggg catagcgata
    18421 gcaacagcat ctattctttt aacaaagcgt tctaatgttg atacatatac agaaataaat
    18481 agttacttag gcaaacttag aggtcaacaa aaacttcttg atggtataga cataatagaa
    18541 ataatataca ttaagagacc ttcaaaagac ttagctaact tacgaaagga gtttaataaa
    18601 actgtaagaa aaaattttct tatcaaactt gcaaaaacct ccgaagcatc tggaagattc
    18661 aacgccgaag accttttaag aatgagaaag ggcaatgttc ctctaaatta taatgttcac
    18721 cataaactat ctctagatga tggtggtact aatgatttcg aaaatttagt attaatcgaa
    18781 aacgaaccat atcataaagt ttttactaac atgcaatcac gaatagctaa gggaatatta
    18841 gtaggtgaaa gcaaaatcac tccctgggcc attccatctg gctcaat
//
LOCUS       DAJVTY010000075        18737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVTY010000075 DAJVTY010000000
VERSION     DAJVTY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18737
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.075"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..719)
                     /locus_tag="OQQ66_002273"
     CDS             complement(<1..719)
                     /locus_tag="OQQ66_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1905191.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPH"
     gene            1186..2418
                     /gene="mdtM"
                     /locus_tag="OQQ66_002274"
     CDS             1186..2418
                     /gene="mdtM"
                     /locus_tag="OQQ66_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCS1905192.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2459..3739
                     /locus_tag="OQQ66_002275"
     CDS             2459..3739
                     /locus_tag="OQQ66_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCS1905193.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            3855..5007
                     /locus_tag="OQQ66_002276"
                     /pseudo
     CDS             3855..5007
                     /locus_tag="OQQ66_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5017..5785
                     /locus_tag="OQQ66_002277"
                     /pseudo
     CDS             5017..5785
                     /locus_tag="OQQ66_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5782..6039
                     /locus_tag="OQQ66_002278"
     CDS             5782..6039
                     /locus_tag="OQQ66_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCS1905194.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5993..6964
                     /locus_tag="OQQ66_002279"
     CDS             5993..6964
                     /locus_tag="OQQ66_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCS1905195.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7032..8204
                     /locus_tag="OQQ66_002280"
     CDS             7032..8204
                     /locus_tag="OQQ66_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1905196.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8217..8772)
                     /gene="kptA"
                     /locus_tag="OQQ66_002281"
                     /pseudo
     CDS             complement(8217..8772)
                     /gene="kptA"
                     /locus_tag="OQQ66_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9022..9704
                     /locus_tag="OQQ66_002282"
                     /pseudo
     CDS             9022..9704
                     /locus_tag="OQQ66_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9701..10162
                     /locus_tag="OQQ66_002283"
     CDS             9701..10162
                     /locus_tag="OQQ66_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1905197.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10175..11347
                     /gene="iadA"
                     /locus_tag="OQQ66_002284"
     CDS             10175..11347
                     /gene="iadA"
                     /locus_tag="OQQ66_002284"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1905198.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11412..12323
                     /gene="hypT"
                     /locus_tag="OQQ66_002285"
                     /gene_synonym="qseD"
     CDS             11412..12323
                     /gene="hypT"
                     /locus_tag="OQQ66_002285"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1905199.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12316..12708)
                     /gene="iraD"
                     /locus_tag="OQQ66_002286"
     CDS             complement(12316..12708)
                     /gene="iraD"
                     /locus_tag="OQQ66_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1905200.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12705..12788)
                     /gene="idlP"
                     /locus_tag="OQQ66_002287"
     CDS             complement(12705..12788)
                     /gene="idlP"
                     /locus_tag="OQQ66_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1905201.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13380..14210
                     /locus_tag="OQQ66_002288"
     CDS             13380..14210
                     /locus_tag="OQQ66_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1905202.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14351..15124)
                     /gene="uxuR"
                     /locus_tag="OQQ66_002289"
     CDS             complement(14351..15124)
                     /gene="uxuR"
                     /locus_tag="OQQ66_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1905203.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15339..16799)
                     /locus_tag="OQQ66_002290"
     CDS             complement(15339..16799)
                     /locus_tag="OQQ66_002290"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1905204.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(16880..18064)
                     /gene="uxuA"
                     /locus_tag="OQQ66_002291"
     CDS             complement(16880..18064)
                     /gene="uxuA"
                     /locus_tag="OQQ66_002291"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1905205.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18404..18608
                     /locus_tag="OQQ66_002292"
                     /pseudo
     CDS             18404..18608
                     /locus_tag="OQQ66_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4385 a         4746 c         4732 g         4874 t
ORIGIN      
        1 tggtgtggca ttcgcctggt aagttcgctg ataaacgcct taaaacgcgc ttcatcgcca
       61 gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt cattagcaca ctctgtaacc
      121 agtaatacta ccagttggtc aataagcccc atcaaatcgc gctggcgaat atgcttttgg
      181 atcaactcca acagggcgac tctgcgatgc tgcacaatct cgtcgtctgg cacgacagtg
      241 acatccacca gcgggaacgc tgcgctataa agtttccgtg cggtagtcgg ggcggcaaat
      301 tcgtccagcc agcacaggga ccaggggtaa ggactacggc taccgtgata aaacagcatc
      361 gggataacca gcggtagcgg ccggcgttta tcatgctcga tatggcgctg catcaccgcc
      421 atggaatatc gcatcaggcg aaatgccata tgaatatcct cgcggctctg atgttcaatc
      481 actacgtaaa tataaccatc accttcacgg gtctttaccg accacagaat atcggagtgt
      541 agcgcccgca atttttcatc gacaaagctg gcggattcca gttttaagct gtcgagatcg
      601 cacagttcac gtaaatcttt gggtaagtgg atctccataa aatcccgcgc ggtgtcaggg
      661 tgagtgagaa aggatttaaa taatgcgtca tgtggcgtgc tggtcgtgaa gtttgtcatg
      721 gcgatccgtc acctgtagat aagatggccc gactttagcg actaacaaaa ccattctcac
      781 ccggcaattt ctcgttcccc gaggtgcttt ccaggcgtct gcaaacgata catttttccg
      841 gaaacaggct cgcgttttac atttcctgta cccggcagat attccccttt ccaccgcgct
      901 cttttgatta accgcaaatt ttaattacac ttaaggtgta tattttctat gcaacccatc
      961 aattaaagag gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc tgacctggag
     1021 tcagaaggta tttctctgtc tgtgtgttca caggcagtgt ggttgattac atgaaattca
     1081 gtacatttgc agtctcgttg cccttctcgc ctgcctttcg tcattaccga cggtattgaa
     1141 tttcgttttc cccgttgggg ttctccgaac aaggagttgt ttgttatgcc acgttttttt
     1201 gcccgccatg ccgccacgct gtttttcccg atggcgttga ttttgtatga ctttgccgcg
     1261 tacctgtcga cggatctgat ccagccgggg atcattaatg tggtccgtga ttttaatgcc
     1321 gatgtcagtc tggccccggc agccgtcagt ctctatctcg cgggcggtat ggcgttacag
     1381 tggctgctgg ggccgctttc cgacaggatt ggccgcaggc cggtgctgat taccggggcg
     1441 ctaatattta cccttgcctg cgctgcgaca atgttcacaa cgtctatgac acagtttctt
     1501 atcgcgcgtg caattcaggg caccagtatc tgttttattg ccaccgttgg ttatgtcacg
     1561 gtgcaggagg cgttcggcca gacaaaaggg atcaagttga tggcgattat cacctccatc
     1621 gtactgattg cgccgattat tggcccgctt tccggcgcag ctctgatgca ctttgtgcac
     1681 tggaaagtcc tttttgccat cattgcagtt atgggtttta tctcatttgt tggcttactg
     1741 ctggcgatgc cagagacggt gaagcgcggc gcggttccat ttagcgccaa aagcgtcttt
     1801 cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc aaccatctct
     1861 ttaagctata tcccaatgat gagttgggtg gctgtctcgc cggtgatcct gatcgacgcg
     1921 ggcagcttaa caacttcgca gttcgcctgg acacaggttc cggtgttcgg cgcggtgatt
     1981 gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga ccgaaccgcg ctttatctgg
     2041 cgtgctgtac ccattcaact ggtcggcctc gcgctgttga ttatcggcaa tctgctgtca
     2101 ccgcacgtct ggctgtggtc ggtgctgggc accagtttgt atgctttcgg gattggtttg
     2161 atcttcccga ccttattccg ctttacgctt ttttccaata acttaccgaa agggaccgtc
     2221 tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg tctcggtcga aatcggccgc
     2281 tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt ggcgggcgtt
     2341 atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc gccaacatca ggctgccgag
     2401 ctggcggagg agcggtgatt tttgcgcgat cctgccgtca ggctctattc ttaacgttat
     2461 gaataaactc attgaactca gacgcgccaa aatgttggcg ctctctttac tgcttatcgc
     2521 cgctgctacc tttgtcgtta cgctgttttt gccgcccaat ttttgggtga gcggcgtgaa
     2581 ggcgattgct gaagcggcga tggtcggcgc gctggcggac tggtttgcgg tggtggcgct
     2641 gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg gcgattatcc cgcgtaataa
     2701 agaccggatt ggcgaaaatc tcggtcagtt cgtgcaggaa aaatttctcg atacccagtc
     2761 gctggtggca ttgattcgac gtcacgaacc ggcgttgttg attggcaact ggtttagtca
     2821 gccggagaac gcccgccgcg ttggtcagca tctgttgcag atcatgagcg gttttcttga
     2881 actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc gcggtccatc gggcgattga
     2941 taaggtcgat ctttccggaa ccagtgcgtt gatgctggag agtatgacca aaaacgatcg
     3001 tcatcaggtg ctgctcgata cgctgatcgc acagttgatc gcccttctcc agcgcgataa
     3061 atcgcgcaag tttattgccc agcaaattgt tcgctggctg gagagcgagc atccgctgaa
     3121 agccaaaatt ttgcctactg aatggctggg cgaacatagc gcggagttgg tttctgacgc
     3181 ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg catcagatcc gtcatgcgtt
     3241 tgatcgcgcc acttttgccc tgatcgacaa gctgaaaaac gatccagaaa tggcagcgcg
     3301 agccgatgcc gtaaaaagtt atctgaaaga agatgaagct tttaaccgct atctcagtga
     3361 attgtggggg gatttacggg aatggctgaa agcggatatc aacagtgaag attctcgtgt
     3421 gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa acgttaattg ccgatgatgc
     3481 cttgcgggca tcgttaaatg gtcacctgga acaagccgcg caccgcgtcg cgcctgagtt
     3541 ttccgcattc ctgacgcgcc acatcagcga tacagtaaaa agctgggatg ctcgggatat
     3601 gtcgcggcaa atcgagttaa atatcggcaa agatctgcag tttatccgtg tcaacggtac
     3661 gctggttggc ggttgtattg ggctaatttt atatttgttg tcgcagctcc cggccttgtt
     3721 ccccctcagc aatttttaga aatccatgaa aaactaagtg gctaaccgcg agagagatca
     3781 aataaaccac actaaaagaa tggtaataat gtggcctctt ttcattatct cccgtggtac
     3841 ggggaaggaa aatcatgtca cttatcaccg atctacccgc cattttcgat cagttctctg
     3901 aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc attccgctgg
     3961 ttggcactta ctgcaccttt atgccgcaag agatcccgat ggcagccggt gcggttgtgg
     4021 tttcgctctg ttctacctct gatgaaacca ttgaagaagc ggagaaagat ctgccgcgca
     4081 acctctgccc gctgattaaa agcagctacg gcttcggcaa aaccgataaa tgcccctact
     4141 tctacttttc ggatctggtg gtcggtgaaa ccacctgcga cggcaaaaag aaaatgtatg
     4201 aatacatggc ggagtttaag cccgttcatg tgatgcagtt gccgaacagc gttaaggacg
     4261 atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt gcaaaaaacg gtagaagaac
     4321 gttttgggca cgagattagc gaagatgctc tgcgcgatgc cattgcgctg aaaaaccgcg
     4381 aacgtcgcgc actggccaat ttttatcatc ttgggcagtt aaatcctccg gcgcttagcg
     4441 gcagcgacat tctgaaagtg gtctacggcg caaccttccg gttcgataaa gaggcgttga
     4501 tcaatgaact ggatgcaatg accgcccgcg ttcgtcagca gtgggaagaa ggccagcgac
     4561 tggacccgcg tccacgcatt ttaatcaccg gctgcccgat tggcggcgca gcagaaaaag
     4621 tggtgcgggc gattgaagag aatggcggct gggttgtcgg ttatgaaaac tgcactgggg
     4681 cgaaagcgac cgagctaatg cgtggcggaa acgggcgatg tctacgacgc gctggcggat
     4741 aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct gcaaatgctc
     4801 agccagatgg tggaagaata tcaggtcgat ggcgtagttg atgtgatttt gcaggcgtgc
     4861 catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgtcagca gcacaacatt
     4921 ccttatatcg ctattgaaac agactactcc acctcagatg tcgggcagct cagtacccgt
     4981 gtcgcggcct ttattgagat gctgtaagga gtggcagtga catattcgat tggcattgat
     5041 tctggttcta ccgccaccaa agggatctta ctggcagacg gcgtgattac gcgccgtttc
     5101 ctcgttccaa caccctttcg tccggcaaca gcaattactg aagcctggga aactctgcgc
     5161 gaagggttag agacaacgcc gtttctgacg ctcaccggct atggacggca actggtggat
     5221 tttgccgata aacaagtgac ggaaatctcc tgtcacgggc tgggcgcacg gtttcttgcg
     5281 ccagcaacgc tgcgcggtaa tcgacatcgg tggtcaggac agcaaagtga ttcagcttga
     5341 tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg gcaccgggcg
     5401 tttcctggaa gtgatctcgc gcacgcttgg caccagcgtc gagcaactcg acagcattac
     5461 cgaaaatgtc acgccgcatg ccatcacgag tatgtgcaca gtgtttgccg aatcagaagt
     5521 gatcagcctg cgctcagcgg gcgtcgcgcc agaagcgatt ctcgccggag tgattaacgc
     5581 gatggcgcgg aggagtgcca atttcattgc tcgtctctcc tgtgaagcgc cgattctgtt
     5641 tactggtggc gttagtcatt gccagacgtt tgcccggatg ctggaatctc acctgggaat
     5701 gccggtaaat acccatcctg atgcgcaatt tgctggcgca attggcgcgg cggtaattgg
     5761 tcaacgagtg aggacacgcc gatgaaagag tttttatttt tatttcactc cacagtcggc
     5821 gtcatacaaa ctcgcaaagc gttgcaggca gcgggcatga cttttcgcgt cagcgatatt
     5881 ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc ccccggcgaa
     5941 gaaatacaat gggtgatccc tgggctgact gagtcaattt attgccagca ggatggtgtc
     6001 tggcgctgca ttgcacatta ccgggtttct ccgcgttaag gattcattaa ggtcgctacg
     6061 tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa aaagtatttc
     6121 gttaagtaaa cgcatctccg ttattgtgat tctttttgcc attgtggcag tgtgtacttt
     6181 cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga attaccacgc
     6241 caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc tgacctggtc
     6301 ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg ttgaaatgac
     6361 caccaatggt gattttatcc gcacaattcc tctggatttt gttaaggatc tcgagacgat
     6421 cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg ccatttatgt
     6481 gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa tcccgctgca
     6541 agactcccca accaattgcg gttttgaagg gttagcttat tctcgccagg atcacacttt
     6601 ctggtttttt aaagagaaaa atccgattga ggtctacaaa gttaacgggc tgttaagtag
     6661 caatgaatta cacatcagca aagacgaggc actacaacgg caatttactt tagatgatgt
     6721 gtccggcgca gaatttaacc agcaaaaaaa tacgcttctg gtgctgtctc atgaatctcg
     6781 cgcactccag gaagtaaccc tggttggcga ggttatcggt gaaatatcac taaccaaagg
     6841 tagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg attgcgatgg atgcctctgg
     6901 taatatttat atcgttagcg aaccaaatcg tttttatcgc tttaccccta aatcatcgca
     6961 ttaacaggct ggcatatttc acctttttgt tattaacttg ttaacaacaa aaatgacata
     7021 caaggttaaa catgccttcg tcctcgcatc ccgtagaacg cttttctttc agcaccgcgc
     7081 ttttcgggat gctggttctg accctaggta tgggattagg tcgcttcctt tatacgccta
     7141 tgttgcccgt cattatggcg gaaggggcgt tttcatttag ccagctctcg tggattgcca
     7201 gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcgtttggc gcatttcacc
     7261 agccgtcgcg cctacgccca tttctgttgg tttctgccct ggcgagaggg ttgttgatcc
     7321 tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcttc ctggcgggtg
     7381 tcgccagcgc cggtatgctg atttttggtt cgacgctgat tatgcagcac acgcgccatc
     7441 cttttgtgct ggcagctttg ttttctggcg ttggcattgc actgggcaac gaatatgttc
     7501 tggcaggcct gcattttgcc ttctcctcgc aaacgttatg gcaaggcgcc ggggcgcttt
     7561 ctggcatgat gctgattgca cttacgcttt taatgccctc gaaaaaacac gccatcgcac
     7621 caatgccatt ggcaaaaacg gagcaacaga taatgagctg gtggttactg gctattctgt
     7681 acggccttgc gggatttggt tatatcatcg tcgcgactta cctgccgctc atggcgaaag
     7741 atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta tcaattgtgc
     7801 caggctgctt tggctggcta tgggcagcaa aacgttgggg ggctttgcct tgcctgacgg
     7861 cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac tctcctctct
     7921 tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca tctctggtga
     7981 tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc tttgtgacgc
     8041 tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc ggcaatggaa
     8101 catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt gctgcgctta
     8161 tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg tttcccttac
     8221 cactcaagaa attcgaccgc aaccgttgat gtcagccaga ctccgttttc cgcttgccag
     8281 aaaggaatgc ctcgtttcgc catttcctgt gctttgacgg ttaagacaac cggcgatccg
     8341 tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgcac gtaatgacga
     8401 tcccctgcaa tcagcccctg ttttttttat ctcgtcaagg aaacggcttg ccgtaccgtg
     8461 gtagagaaac tgtggcggtg ttttttcggc aaaggaaata gcgacctgcg aagtcgaatg
     8521 cccctgaacc gcgcggatgc acctaccatc actggaataa ctaaaacgct ttttatcgct
     8581 ggttgcgacc acggtatcca gcaatgcacg ggttaatcgc ttgcccgctt tttgggcaca
     8641 aagtatgagc ttatcgatat ccgcccatcc ttcacggtcc agtacgatac caatcgcctc
     8701 cggtttgtgt cggaggacaa aacttaaaaa cttgctcgta tccgccagct ctttttcgtt
     8761 gtgctttgcc atctcgtttt cgtccctgga ttttctgttt ttatttacca atgtcattgt
     8821 cactgggttt atgaattttc tgcaactcaa tcctgcgcag aaccttttcc acacagcgta
     8881 atgtttcccc tatgcaaaaa acgcatagcc agaaacatac ttgcattttc cccataccca
     8941 catggctgat aaggtttttc agttccagga attttcctgg ctcaagcaac acacaaacac
     9001 aaaaaaacaa tggggattgt tatgacacaa caaggggatg ctgtagccgg ggagctggcg
     9061 acagaaaaag tgggcattaa agggtatctg gctttttttc tcaccataat cttttttccg
     9121 gtgttttttc cggaacagac agctggtggc gcgtttttga tttttccgtt ctgaacggat
     9181 cgtttgggca actgcctggt gcgaacggag ccaccacctc atttcgcggc gcgggtggtg
     9241 cgggggctaa agatggcttt ctctttgcgc tggagctggc accgtcagtc attctctctc
     9301 tggggattat ttcaattacc gatggtcttg gtggattacg cgccgcgcag cagttaatga
     9361 cacctgtgct aaaaccgttg ttgggcattc cagggatctg ctcgctggcg ctgatcgcta
     9421 acttgcaaaa cactgatgcc gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa
     9481 ttaccgaacg cgataaagtg attttcgccg catatcaaac cagcggtagc gccatcatca
     9541 ccaactactt ctcttccggc gtcgcggtgt tcgcctttct gggcacgtcc gtaatcgtac
     9601 ctttagccgt catcctggtg tttaaatttg tcggagccaa cattttgcgc gtctggctta
     9661 acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa
     9721 aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa
     9781 cctgctgcca aacgtggtga tggcgtttgt catcattcag gcgctgaaaa ttaccggcct
     9841 gctcgactgg gtggggcata tctgtgaacc cgtaatggcg ctgtggggat tacccggtga
     9901 agcggcaacc gtccttttag ccgcattaat gagtatgggg ggcgcagtcg gtgttgccgc
     9961 cagtctggca accgctggcg cattaaccgg tcacgatgtc accgttttac tgcccgcgat
    10021 gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa
    10081 tgccaaatat tacccgcata tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg
    10141 ggttatgcag cttattgttt aacaaggagt taccatgatt gattataccg cagccggttt
    10201 taccctgctg cagggagcgc atttgtatgc gccggaagat cggggaattt gcgatgtcct
    10261 cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc ccttctgaca ttgtaccgaa
    10321 ctgcacggtt gtcgatctca gtgggcagat cctctgccca ggttttattg atcaacacgt
    10381 ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg cgcacgccgg aagtggcgct
    10441 aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt ctgctgggca ccgactctat
    10501 ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag
    10561 cgcctggatg ctgaccggcg cttatcatgt cccttcccgc actattacgg gttccgtgga
    10621 aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg
    10681 ttctgccgca ccggacgttt atcacctggc taatatggcg gcagaatccc gcgttggcgg
    10741 tttgctcggc agtaaacctg gcgtcaccgt gttccacatg ggcgacagta aaaaggcgtt
    10801 acagcctatt tatgacctgc tggaaaactg cgatgtgccg atcagcaagc tgctgccgac
    10861 ccacgttaac cgcaacgtac cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg
    10921 caccatcgat atcaccagca gcattgacga accggtcgcc cctgccgaag gtattgcccg
    10981 cgccgttcag gcgggtattc cgctggcacg cgtcaccctc agctccgacg gcaacggtag
    11041 ccagccgttc ttcgatgacg aagggaattt aacccatatc ggtgttgcag gttttgaaac
    11101 gttgctggaa accgtgcagg tgctggtcaa agactatgat ttcagtatca gcgatgccct
    11161 gcgcccgctc accagtagcg tagccggttt ccttaacctg agcgggaaag gcgaaattct
    11221 gcctggcaat gacgcagact tactagtcat gacgccagaa ttgcgcattg agcaggtata
    11281 tgctcgcggc aaactgatgg tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac
    11341 ggcttaaacg gttgtcagcc gccctaatgg acgtagaatg cccgcaaggg cggctgacag
    11401 agtaaaacgt aatggatgac tgttgtgcgg ttttgcataa tattgaaacc aaatggcttt
    11461 atgattttct gaccctggaa aaatgccgca atttttccca ggcggcagtc agtcgtaacg
    11521 tctcgcaacc ggcattcagc cgccgcatcc gcgcgctgga acaggcgatt ggcgttgaat
    11581 tgtttaaccg ccaggtgacg ccgctgcaac tctcagaaca agggaaaatc ttccattcgc
    11641 agatccgcca tctgttgcaa cagttagaaa gtaaccttgc agagctgcgt ggcggcagcg
    11701 attacgcgca acgtaaaatc aagatagccg ctgcacactc tctttccctc ggtctgttac
    11761 cgtccattat cagccagatg ccgccgctct ttacctgggc gattgaagct attgatgtcg
    11821 atgaagcagt cgataaactg cgtgaagggc aaagtgactg tattttttcc tttcacgacg
    11881 aagatttgct ggaagcaccg tttgaccaca ttcgcttatt tgaatctcaa ttgttccccg
    11941 tttgcgccag tgacgaatac ggagaagcaa tttttaacct cgcgcagcca cactttccgt
    12001 tactgaatta cagccgcaac tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc
    12061 acagcgagtt aagtttcagc accttttttg tctcttcgat gagcgagcta ttaaagcagg
    12121 ttgccctcga cggctgtggg attgcctggc tgccggagta tgccatccaa caagaaattc
    12181 gcagcgggca actcgttgtg ctgaatcggg acgaactggt gatcccgatt caggcctacg
    12241 cataccgtat gaatacccgc atgaatccag ttgccgaacg cttctggcgt gaactgcgcg
    12301 agctggagat tgtgcttagc tgacattctc aagcgtcgca ctgcgccaac cgccattatc
    12361 ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac agcattatct caaggctgat
    12421 cgccagagtg tggcagtacg cttctttcaa attaacctgc aacgccgcaa tccgtggctc
    12481 atgaaataac agtgcagaac gcagctgacg gcaataccag ctctggagat gtttgtcgct
    12541 cgggttatgc acttgccact cgggcatgcc tgaaacaagt aatgacggta atgccgaatg
    12601 aggagaatta aaaagagtga ggagatcgct gcataccgat ttacgcagca atgacgtttt
    12661 attgcctgaa atttcaggta aaacagcctg aagtgattgg cgcatcattt tgcgcactcc
    12721 tgacgtttag caacatatgc ggcttgccct gaacaccggg caccactgta ttgatgctca
    12781 ttttccatag ccattccctt ttgttcatta catccatgtc agtctgagac aggcctgaat
    12841 cctgtcagca aagtataaaa ccttacttgc ctatgaatat ctactgactt ttcaggaaaa
    12901 aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa ccgctagtat attctgaata
    12961 ttccgaatag ttacgtagat gaaaatactt gtcaccgccg ctacttaatt ccggtgggaa
    13021 attatttatt tccgcataat cagccatgta ttatttgaaa caattagttt gtaaataata
    13081 taaattgata acatatcaaa tacatcaaaa ataccattca taggcattat taatatagac
    13141 atcaatatag caacatcaaa ttccaattag taataagata tgctttttaa ttgcatgaaa
    13201 ataatttcaa atgagcgttt tttgatactt tagagtgttt cctacccttt aatatcaccg
    13261 tgtagagttg atatcactac aacagaaaca tgcatgtcat ttttttactt cttttttact
    13321 acagaaaaat ttatctattt ttctaaacat cggtgaaata ctcataaaag gtaactatta
    13381 tgtctatgcc attaagcaat gcccttcaga gtcaaattat tacagataat cacttccttc
    13441 atcatccaaa agtcgacaat aagttaacac gtgaatatga atatacacgc atggatacgg
    13501 aaaatatcta tcttctgcct cttactcgtg gaaataacca taattacgat ggaaaaagcg
    13561 ttgtcgaaat tagaaaactt aatattggca aacagtcctg gccatttaat tatgcgacag
    13621 atacatgcag agaattcaat ggtattacaa caacaggacg tatgctctat cgaaacttga
    13681 aaatcacctc agctttagac gaaatatatg gtggcatatg taaaaaggcg catgctgcta
    13741 cagagttagc agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg
    13801 aggactatac agttcatgaa ataacattag gccctggtta caatgttcct ggttatgccg
    13861 gaaccacgat aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg
    13921 agcagccaag aattgatatc tatattgatc agataataac tgtctcaggg gtggcaaatt
    13981 cttccggatt cgcccttgcc gcattactta atgctaatat tggaatggga aatgacccaa
    14041 taattggtat agaagcctat cctggtaccg cggagatcca tgcaaaaatg ggatataacg
    14101 ttatccccgg tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc
    14161 cagaactgtt tgaattaaaa aatggtgaat ggaattatat tggcaaataa ccactgagaa
    14221 gaatgagtac taacattcag gttatataga tatttaaaca ataccctgca atttacaaag
    14281 taatactcat tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta
    14341 tcataatcag tcaggcgtcg actttatcca gcggccatga atcaaacaga tagccgtcaa
    14401 aataaatatc gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct
    14461 gccacattgc cagcttcgcc gctcgggcat cttttttgat taacgcggca aggatctgtt
    14521 tgtgatcgcc caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga
    14581 tccacattgg attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc
    14641 tgttatgcgt tgcttcagca atggcgagat ggaactgcat gtcaccgctt tcgctgctac
    14701 ccggcgcgct ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga
    14761 tatcttcgcg ggtagcctgc agggcggcaa attccgcgat gttgctctca agaagctgtc
    14821 gcgcctgcaa caattcaaag gggccggcat cattgcagac gttggcatcc ggactgtctg
    14881 tgttgtggct gcctgagcta tcaagaacat agataccggc accccggcgc acttccacca
    14941 gccctttgat ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca
    15001 tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga
    15061 tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtggcagatt
    15121 tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg
    15181 ccttatccgg cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta
    15241 gcactggaag ttagcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca
    15301 gtttgattga ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg
    15361 cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca ccgcgccaac aaagtcggca
    15421 ttctgcggca gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct
    15481 gcgccctgat actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca
    15541 atggcattac cctgctcatc cacgccctgg gtgtaacgca tccagccagc cacgcccagc
    15601 gccaggtgac gccagctgcc gccattttgc aggtgcagac gcaccgggtc cagcagacgc
    15661 tgcggtaact tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg
    15721 ttgctgaaac gctcgatcag cagcgtcgca taggccttca ggtccgtacc ctccggcata
    15781 gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg
    15841 ttagtcatgg tgtcggcaat agtttcatag ccgccgaggt aaccgaggta tgccaggaaa
    15901 gagtggctgc cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg
    15961 aactgtgcgc ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc
    16021 cactgacgga acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca
    16081 atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg
    16141 aaggtgacgt tctcttcaat ccatgccgcc agctgcgggt cacgtgcctg agccagcccc
    16201 agtaccgcga ccttcgccac atgaccgttt tcacgcacgt tatcgcagga catcaccgta
    16261 aatgctttca gccctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg
    16321 gacttcggcg cagtcgggtt ttctagatcg tgcttgatca gcgggttatt gagatccagt
    16381 tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc
    16441 gtttgcggac gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc
    16501 gcttctttca tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg
    16561 gtgtacagca gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt gcctggcatc
    16621 aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac
    16681 agcgcctgat gtgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga
    16741 cgagaatgat cccatgacgg acgggcaacc ggcagattgc tgtcaacaat agtagtcatc
    16801 gtaaactcca gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc
    16861 gcagccatgc cggtggatat taacggctaa agaaagcgcg ctggatcgcc agttcgacac
    16921 cgcgaacttc ggccaggcct ttcagacgac caattgcgga gtaacctggg ttggttttct
    16981 tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt
    17041 tgccttccgc tttacgacgg tgttcttctt caacaatcgc tttcaccact tcgtacatat
    17101 caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg
    17161 tggagcgcag atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat
    17221 cgttgtcagc acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt
    17281 ttacggtatc aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaggccga
    17341 ggatcgggcg cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa
    17401 ctggaataat cgctttcagg aagacggcaa agttttcgcg cagcttcgct ttatcgatat
    17461 ctttgtacag ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg
    17521 gcagaccggc aatgatgtta cgcgtcagac gcgctttgtc ttcatcgctc atagtggcga
    17581 agcgttcagc tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt
    17641 tcaggatatg catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc
    17701 cgtctggcag cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt
    17761 agcacacggt gcgaatgccg cactgcgcca ggttgcgcag agtctgctga tagttagcga
    17821 tccactgctc atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta
    17881 cgacagacca caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta
    17941 cggaccatac ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg
    18001 cctgacggac atcagctaaa gaaaccagat cgtttgggcc gtaccagcgc caggtctgtt
    18061 ccatgatttt tcctctttca catagtgggt tccacagcaa agagacgcgg aaccctggag
    18121 ttgtttaggt atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg
    18181 gcgtataagt tctacctttt ggtaagatat atcaacccaa atcctgaaat tggttaacca
    18241 aatcacaaga atttcactct catggttgtt gctgcaaatt gcagcaaaag gtaatcgatg
    18301 gttattgatc agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg
    18361 tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac gttatgcatg tgcttaacat
    18421 tctctgggta gtattcggca ttggtctgat gctggtactg acaacgaccg caggttcaga
    18481 cacattcact gcattagcaa tgcctcttag attattttga cctgtcagta aaagacacgt
    18541 tgaaaacctg gggtctgctg gaactggtca actccgtggt tgggctgatt attagcaagg
    18601 tagcgtaaaa gaacaccaga gcctgccaat ggcaggctct gactgatgac aaagtctaaa
    18661 aatgtgcccg gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac
    18721 cagcaaagtg cgctttg
//
LOCUS       DAJVTY010000076        18663 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVTY010000076 DAJVTY010000000
VERSION     DAJVTY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18663
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.076"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..262
                     /locus_tag="OQQ66_002293"
     CDS             <1..262
                     /locus_tag="OQQ66_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="member of the major facilitator superfamily (MFS)
                     of transporters; unknown function; may be associated with
                     transport or processing of arabinose polymers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1905206.1"
                     /translation="PLQILLLQNAKGGELLGAAGGQIAFNLGSAVGAYCGGMMLTLGL
                     AYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKPLG"
     gene            complement(405..1313)
                     /gene="mak"
                     /locus_tag="OQQ66_002294"
     CDS             complement(405..1313)
                     /gene="mak"
                     /locus_tag="OQQ66_002294"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1905207.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            1438..2349
                     /gene="rdgC"
                     /locus_tag="OQQ66_002295"
     CDS             1438..2349
                     /gene="rdgC"
                     /locus_tag="OQQ66_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1905208.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(2427..2510)
                     /locus_tag="OQQ66_002296"
     CDS             complement(2427..2510)
                     /locus_tag="OQQ66_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905209.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(2507..2848)
                     /locus_tag="OQQ66_002297"
                     /pseudo
     CDS             complement(2507..2848)
                     /locus_tag="OQQ66_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2996..3280)
                     /gene="ppnP"
                     /locus_tag="OQQ66_002298"
     CDS             complement(2996..3280)
                     /gene="ppnP"
                     /locus_tag="OQQ66_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1905210.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(3352..4029)
                     /locus_tag="OQQ66_002299"
     CDS             complement(3352..4029)
                     /locus_tag="OQQ66_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1905211.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(4287..4478)
                     /gene="yaiA"
                     /locus_tag="OQQ66_002300"
     CDS             complement(4287..4478)
                     /gene="yaiA"
                     /locus_tag="OQQ66_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1905212.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(4528..5052)
                     /gene="aroL"
                     /locus_tag="OQQ66_002301"
     CDS             complement(4528..5052)
                     /gene="aroL"
                     /locus_tag="OQQ66_002301"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1905213.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(5235..5693)
                     /locus_tag="OQQ66_002302"
     CDS             complement(5235..5693)
                     /locus_tag="OQQ66_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1905214.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5813..6622
                     /gene="proC"
                     /locus_tag="OQQ66_002303"
     CDS             5813..6622
                     /gene="proC"
                     /locus_tag="OQQ66_002303"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1905215.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6639..7754)
                     /gene="adrA"
                     /locus_tag="OQQ66_002304"
                     /gene_synonym="dgcC"
     CDS             complement(6639..7754)
                     /gene="adrA"
                     /locus_tag="OQQ66_002304"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1905216.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7856..8176)
                     /gene="psiF"
                     /locus_tag="OQQ66_002305"
     CDS             complement(7856..8176)
                     /gene="psiF"
                     /locus_tag="OQQ66_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1905217.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(8295..9710)
                     /gene="phoA"
                     /locus_tag="OQQ66_002306"
     CDS             complement(8295..9710)
                     /gene="phoA"
                     /locus_tag="OQQ66_002306"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1905218.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9811..10071)
                     /gene="iraP"
                     /locus_tag="OQQ66_002307"
     CDS             complement(9811..10071)
                     /gene="iraP"
                     /locus_tag="OQQ66_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1905219.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(10298..10455)
                     /locus_tag="OQQ66_002308"
                     /pseudo
     CDS             complement(10298..10455)
                     /locus_tag="OQQ66_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10533..11627
                     /gene="ddlA"
                     /locus_tag="OQQ66_002309"
     CDS             10533..11627
                     /gene="ddlA"
                     /locus_tag="OQQ66_002309"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1905220.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11651..11863)
                     /locus_tag="OQQ66_002310"
     CDS             complement(11651..11863)
                     /locus_tag="OQQ66_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1905221.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            12123..12431
                     /locus_tag="OQQ66_002311"
     CDS             12123..12431
                     /locus_tag="OQQ66_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1905222.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(12490..13584)
                     /gene="yaiW"
                     /locus_tag="OQQ66_002312"
     CDS             complement(12490..13584)
                     /gene="yaiW"
                     /locus_tag="OQQ66_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1905223.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(13597..14817)
                     /gene="sbmA"
                     /locus_tag="OQQ66_002313"
     CDS             complement(13597..14817)
                     /gene="sbmA"
                     /locus_tag="OQQ66_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1905224.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            15169..16326
                     /gene="ampH"
                     /locus_tag="OQQ66_002314"
     CDS             15169..16326
                     /gene="ampH"
                     /locus_tag="OQQ66_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1905225.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(16327..16950)
                     /gene="iprA"
                     /locus_tag="OQQ66_002315"
     CDS             complement(16327..16950)
                     /gene="iprA"
                     /locus_tag="OQQ66_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS1905226.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWKNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINCLPSEY"
     gene            complement(17038..>18663)
                     /locus_tag="OQQ66_002316"
     CDS             complement(17038..>18663)
                     /locus_tag="OQQ66_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1905227.1"
                     /translation="VNNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDS
                     YSTVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADH
                     NGNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDN
                     GTELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKA
                     DLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHG
                     LADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKG
                     DMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTN
                     SDAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYEL
                     GVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFS
                     FTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         4648 a         4831 c         4475 g         4709 t
ORIGIN      
        1 accgctacaa atattgttac tacaaaacgc caaaggcgga gagttattag gtgccgcagg
       61 tgggcaaata gcgtttaacc tcggtagcgc cgtcggcgca tattgcggtg gtatgatgct
      121 gacgctgggg ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc
      181 gatgtcgtcg ttgctgctgt atggtcgcta taagcgccag caagcggcgg atactccggt
      241 gctggcgaaa ccactggggt aggttatagt ctcggtgttc attacttatt gccggatgcg
      301 gcgtgaacgc cttatccgcc ctacgcggtt ctggcacatt ttgcaggcct gataagacgc
      361 ggcaagcgtc gcatcaggca tgatgcgcca attgcctacg ttttttactc ttgtggccat
      421 aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct tcgccttacg caccggcgtt
      481 tcacattcgc cgccgaagac aaattgttta atcaactgcg gaaccgtttg atataaacgg
      541 tctacattgc tcatcccccc ccccaggaca atcacatccg gatcgagaat attcacgaca
      601 tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc cgctaccgga
      661 tcgctttctt caaccaggcg gataatttca ctgcctttca gcgcatgtcc gctcaaacga
      721 cgataatcca tcgcgaatcc cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg
      781 caataacaag ggacttcctc gcgataacgc agttcgtctt cgtccatcca cggtagcgga
      841 ttgtgtcccc actcacctgc cgtgccattg ccgctgatat gcgcccgccc attgaatgcc
      901 acgcccgcgc cgcatcccgt gccgataatc acggcaaata ccgtctgcgc tcccgctgcc
      961 gcgccatcta ctgcttctga aaccgccaga cagttagcgt catttgccag ccgcacttcc
     1021 cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac cgttgagcca ggttgaattg
     1081 gcattcttca ccacaccggt gtaaggcgaa attgagccag gaatgcccat acctaccgtt
     1141 ccgcgctgcc ccgtcgcctg ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc
     1201 tgccggtaat catcacgcgg cgtgggcaga cgatggcggt acaactgctc ccctgcatcg
     1261 cccagtgcaa tcacttcagt tttggtgccg cctaaatcga tacctatacg cacggtactc
     1321 tccttatttt tttcaatatc aatagcgtag agacggacaa ccggattggc aatgcaaggc
     1381 cgccgacaat tcgttatcat gcccgctaaa tttaacaaca aggccgtgga aattatcatg
     1441 ctgtggttca aaaatttaat ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa
     1501 gagatggaaa aacagctagc ctcgatggca tttaccccat gcggcagcca ggacatggcg
     1561 aagatgggct gggttcctcc gatgggatcg cacagcgatg cgttaacgca cgttgccaat
     1621 ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc tcccgtctcc ggtgattaag
     1681 caggcgctgg aagcgaaaat cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa
     1741 accgaaaaag attcgctgaa agacgaagtg ctgcactctc tgttgccgcg tgctttcagc
     1801 cgttttagcc agacaatgat gtggatcgac acggttaacg gtttgattat ggtggactgc
     1861 gccagtgcca aaaaagcgga agatacgctg gcactgctgc gtaaaagcct ggggtcgtta
     1921 cctgtggtac cattgagcat ggaaaacccg attgaactga cgctgaccga atgggttcgc
     1981 tccggtagcg cggcacaggg cttccagctg cttgatgaag ccgagctgaa atcgttgctg
     2041 gaagatggcg gcgtgatccg cgcgaagaaa caagatctga ccagcgaaga gatcaccaat
     2101 cacatcgaag ccggaaaagt ggtgactaaa ctggcgctcg actggcagca gcgcattcag
     2161 tttgtgatgt gcgacgatgg ttcgctcaag cgtctgaagt tttgcgacga gctgcgcgat
     2221 caaaacgaag atatcgaccg tgaagatttc gcccagcgtt ttgatgccga tttcatcctg
     2281 atgactggtg aactggcagc gttaattcaa aacctgattg aaggattagg tggcgaagca
     2341 caacgttaat tgctgatttt cctttaatgc cggatgcgac gcctgccgcg tcttatccgg
     2401 cgtacgaagc cacaccaggg atataattat tcgctacggc ggacaataat ttttagcgca
     2461 gcaatattat gcgttttatg ctgtaacttg ctccatggac gttgtgtcat tgtttttcct
     2521 caagccagtg acgcgcttca ctgcgaattt tccgcattgc cccggttttc actaaatccc
     2581 gtaacagcga aagatcgggt tgttcgacaa ataagagtaa tgatggatcc tcttttaacc
     2641 acgtcgtttt cacatcggca gcgagctgtg gattattcat agctagcgac cgatgccgag
     2701 actcggtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat gccgtttggc
     2761 gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct gatttatctt
     2821 ttatcgtagc cagttccgca acaatatctg gagatcttca ctgatattta tatcaacctt
     2881 gatgaattat gaatatatcg gaaattgaaa aaatcgcagc aaaggaaata ttattatggg
     2941 ttaaagagag gatatttcag aaaaatgccc tcccgttcaa ggggaaggcg aggaattaca
     3001 gatagcggca cagataagag gtgggttcgg caacttgcag atgaaactca ctgtgaccgg
     3061 gaacattaaa caccgaaccg gcttcataca cctgccagtc ggtcgcatca ggcagtaaca
     3121 cattcagcgc gccactgatt accgtcatct cttccggctc agcggtgctg aaggtgtatt
     3181 cgccttcaac cataacaccc acgctggcgc gaccagtgct gctgctggaa aagccgattg
     3241 atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc tgtctggtag
     3301 ttgaacatct acttcactat aggggccaga ggcgctggct gtcacgcaaa attacaccag
     3361 taattccgca gccagccgtg caatcaatac gttagacagc aagacaggaa catcgagctg
     3421 tttttgcagt aaatcgcgat gacgttggtg aaatcctaaa caatccagca tgatgacatc
     3481 agcccctttt gccagtaatt ctttccctgc atcaatgatt ttttgttctg aatcatgaat
     3541 ggggttaccc aatgaaaata ccggcgattt ctgcaaaatt tgccattttt gcgcctgaac
     3601 gggcagcatc tcctcaaccg gaacgataac ccccacctga tgatcttcaa caatagagga
     3661 aaccagtgga ggcaatattc gcgacggctc aagaaagatc gtattacgcg cagtcatact
     3721 actaatgttt gctgtactca ttaataaaat gacgtcataa ccctgattat cgagcacttc
     3781 aaccacacct tgcaggtctc gctccacttt acgacgcgaa acatgggcca gctggttgtc
     3841 atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg ccatcacttc
     3901 ttcacgactt aacttcccca gcaggctatg atgggaaata ttatcttcgt caatgtattc
     3961 cgtcaggagc ggcaaaacct cctgcatagg tacaatgccg atggtcagga tcgccaacga
     4021 cgcactcatt gttcatccac cttacttttc tttcgtcgtt aattaccggg caagtgtgaa
     4081 gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag cagacgtgca
     4141 cggcataatg taaagattcc aaatgattcc agtaatggat tgttattgtt taatattcta
     4201 attattagaa aaacatgaat tatgaaaaat gtgacgcaga tcccttgtag tggcactcag
     4261 aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata gcgttttttc
     4321 gcatccatcg cttcctgagc ggtcggatgt tcactgatca acgagtctgg tttaggatga
     4381 tcggctctga gttggtacca ggttaccgtc tgtcctggct ttcctttttc aatcgtcact
     4441 atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta tttcacggaa
     4501 tgaacgttaa gtataggcgc tctaaaatca acaattgatc gtctgtgcca gggcgctacg
     4561 aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat gcgcaacttc
     4621 gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca gcggttttcc
     4681 cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa ccaggactga
     4741 tactggcgca cacaaataaa ccacgatccc gttattttgc atgaagtgac gattaaattc
     4801 cgtcagaata atgccgccgc ctgtagcgat aactgtggat ggcgcagtta ccgcttccag
     4861 cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc ctttcgacga tctccgcgac
     4921 cgtcatattg agctgtgatt gcaaccactg atcggtatcg acaaaccgac ggttaagcga
     4981 atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga tcagaaaaag
     5041 aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc cggtgtcatg
     5101 agaatagcga tcattccatc aaactagtac aatttcgatt gtaaagaaaa aattccactt
     5161 aaagtgaaaa tctcaataca tcccttacta taccaataaa tattcaagaa tgaagtgtaa
     5221 ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt ctccagctcc
     5281 gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt ctggatccca
     5341 ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat ggtcgctggc
     5401 gtgtaacgtt cgccgcgcgg attaagcgcc gctgcgcctt tctcgatggc ttcagcagcc
     5461 aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac aatttcgtta
     5521 tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga tggcggcacg
     5581 cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc cgcgcgatac
     5641 aaaatctctt taattacatt gggacacgcg tcagcatcca cccaaattgt cataaagtca
     5701 tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata gttctgttta
     5761 tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg caatggaaaa
     5821 gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg gtctgattgc
     5881 cagcggtcag gtgcttccag ggcaaatctg ggtatacacc ccctccccga ataaagtcgc
     5941 cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag tggcgcaaat
     6001 cgccgacatc atttttgctg ccgttaaacc tggcatcatg attaaagtgc ttagcgaaat
     6061 cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggtg tcacgctcga
     6121 ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc cgaacactcc
     6181 cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa ccccagaaga
     6241 taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa ttgctgagcc
     6301 gatgatccac ccggtagtcg gtgtgagcgg ttcttcgcca gcctacgtat ttatgtttat
     6361 cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg cgtataaatt
     6421 tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag aacatccggg
     6481 ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaagcgg tacgcgtact
     6541 ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta tggaaaaatc
     6601 agaaaaactc agcaaatcct gatgactttc gcctgacgtc aggccgccac ttcggtgcgg
     6661 ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg ccgatttcaa ccactcacga
     6721 tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa agttacctgt
     6781 ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca cccgtaacat ggcggtaatg
     6841 gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc aaaccgacca
     6901 atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac aatcgcttca
     6961 tcgcccacat catgacccca ggtatcgttg atgctcttga aatggtcgat atcgataatc
     7021 agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg taacatagtt
     7081 tcccaatgac gtcggttata cacgcccgtc atgccgtcgc gggtactcat gacctgcaac
     7141 ctgcgtttat gttccgccag tttggttgcc gtctggtagc tgacccagcc aaacagcaga
     7201 ggataaacga caataatggg aagggagagc caccattcca gcggcgcact attgaacgac
     7261 acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa caccagaccc
     7321 gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacataat catcaacata
     7381 gcggtggaag gcagcacgtt tacgcccatt acgcctaccc acattcccgc taatactgca
     7441 tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct cgctatctgc
     7501 caggctaaat gcggccagac gaacgcccag ccgaccaaca ctagccacca ccagcccggc
     7561 ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag gccaaccgca
     7621 cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc gttttgagga
     7681 gttaaaggag gttcctcccc atgcgccgcc gcttttttga aaaagttttc atcattcatt
     7741 atttttggga acattcagaa acaattttcc caaattatag agacggggct aaaggcagaa
     7801 gtatgaaatt tcgggtgagc cactttcgta gctcacccgt tagcactatc aggtatcagg
     7861 cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc agagattgtt
     7921 gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa cttttttcac
     7981 caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt
     8041 tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgtggggtta
     8101 acgttctctc ggcagcgccg actgtggtta aaaaaaccag accgaaaagc aaggtaacca
     8161 gtaatgtaat tttcatagca ccatccctct tcatgtttta accatgagcg tatgcgcccg
     8221 agatttgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac
     8281 tgccgggcgc ggttttattt cagccccagg gcggctttca tggtgtagaa gagatcggtc
     8341 tggtcggtca gtccaacgac attggcggca tgcgggccat acgccgcaat acgcaactga
     8401 ctgccggtat gttcttgtga atcctcttcg gagttcccgt agctcatcac catcactgcg
     8461 ccatctttgg tatttagcgc ctgggtgagg cccggagctt tggtatccgg cgcgacaatc
     8521 tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttgccatc ctttttagcg
     8581 aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa
     8641 ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag gaaaaatcct
     8701 ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgcgccag ggttggtaca
     8761 ctgtcattac gttgcggatt aggcgtacag gtgactgcgg gcttatcgat attgccgtgg
     8821 tacgttgctt tcggtcctag ccagcgcact ggcatattgc cgtcagcaaa cagtccaagc
     8881 agaggttttt gctgattcgc ttccgtcacc gcatttagcg aggcggcatc gctgaccaac
     8941 tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc accggcggtt
     9001 gccgtttcag caaaggtttt tgcgccaccg ccaagcgtaa cgtcggcacg agcattaagc
     9061 agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg acatttttca
     9121 ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag cgcagcgggc
     9181 gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc ggcttttgcc
     9241 atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc gccgttatag
     9301 gttttgacac cggttgacca ggcggttgct gatgcagccg aatcggtgac gtagtccggt
     9361 ttgccggttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg taaggcatcg
     9421 atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggctgt aatttccgaa
     9481 tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc gctaagagaa
     9541 tcacgcagag cggcagtctg atcacccgtt aaacggcgag caccgccggg tgcagtaata
     9601 tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc ttttgtcaca
     9661 ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac tttattttct
     9721 ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg ccgtgacaac
     9781 tttatgacag ctgttgaaaa gattaacttt ttactgacga ggatgcttca ataacttctt
     9841 tacataattg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcag gctttacctc
     9901 gtaaagagcc ccctccacct gttcaatcaa gcgctgttgg tcattttgtg ccatattgcg
     9961 aagcattgca gtgacgataa tctccaaagc ttctacctgc gcgcacagtt ctttcgactc
    10021 ttcttctttt tgggcaagct ttaataacaa ctcagcaatg agatttttca tgtctgtatt
    10081 tccttatcca acgtatggag aagttaacat tatgtttcac aacttcatag tgtctggaag
    10141 tattattttt atgacaaagg aaatactttg cgcaaatgtt ctttgtttga ttaccagcga
    10201 aacgtttctc atatttaaat aattaaccac acaaagaaca tgcctttaat tatcaacctt
    10261 tattataaac aaaagttatg aaatatattc tctgcgttta cttacaacta agagaaaaaa
    10321 ctcaccgcat ggccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat
    10381 ctcgcgcaca tctttgtaat aacgcacttt cttcaccatt acgttgcacg ctttgtcagc
    10441 agaagattgc aacattctgt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat
    10501 gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt
    10561 ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg
    10621 ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc
    10681 acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc
    10741 gcccttcggc gactagcctt gcgcaggtgc caagtaaaca tgagcatcag cttatcgacg
    10801 cgcaaaacgg tcagccgtta ccgacggtgg atgtcatttt cccgattgtc cacggtacgc
    10861 tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt
    10921 ctgatgttct ggcttcagca gcctgtatgg ataaagatgt taccaaacgt ctgctgcgcg
    10981 atgccgggct gaacattgcg ccatttatta ccctgacgcg cgccaatcgt cacaacatta
    11041 gttttgccga agtggagtct aaactgaggt taccactgtt tgtaaaaccg gctaatcagg
    11101 gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc
    11161 tggcgttcga attcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg
    11221 aatgcgcagt tctgggcaac gacaacccgc aagccagcac ctgtggcgag atcgtactca
    11281 ccagcgactt ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag
    11341 ttccggcagc cattgcgcca gaaatcaacg ataagatccg ggcgattgcc gttcaggctt
    11401 atcaaacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg
    11461 aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga
    11521 agctgtggca agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg
    11581 cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc
    11641 gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg
    11701 ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg
    11761 cttcaaatcc cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg
    11821 catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga
    11881 aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa
    11941 ttgttaataa atttaactta aggttgccat tttgtgactc acaacgaaga ttcacgcttc
    12001 gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc
    12061 agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt
    12121 ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt
    12181 caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc
    12241 aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg
    12301 aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc
    12361 cgtttttagt aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc
    12421 gccgccagta agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt
    12481 cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca
    12541 ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc
    12601 gcggggtagt gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc
    12661 cgtctcctca aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct
    12721 catccccagt tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact
    12781 gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgaccgc
    12841 gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta
    12901 tatcggtgcg ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc
    12961 gccgcggcga ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt
    13021 ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt
    13081 aagcgaacca aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc
    13141 gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg
    13201 cgatttgatt tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg
    13261 acggtcaatc tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata
    13321 atttgactcc tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag
    13381 cccctgactt tcaaaggtgg tggcgagatc tttcgcccag gcatcgcggt ctttcacgct
    13441 cgcaggcatc ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa
    13501 cggttgcgga gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga
    13561 agatagatga ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc
    13621 ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat
    13681 caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc
    13741 aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg
    13801 aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt
    13861 gaaatacata tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc
    13921 gcgtaccgta ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg
    13981 gtaggcggcc tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac
    14041 tgccagcaat ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg
    14101 aatgtgcccg ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag
    14161 gaaggcgacc aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga
    14221 agcaaaacgc atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg
    14281 ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact
    14341 gacaaagaag ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac
    14401 gcccacttcg cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg
    14461 aatcagatca tagaacggtg cataccaggc gttgacggcg actccgactt ccaccaaaaa
    14521 ccaggtgacg aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg
    14581 gctgtagata aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa
    14641 aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc
    14701 ggtgatacgc gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc
    14761 ccaaacaaag gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg
    14821 cactcctgct aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac
    14881 aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag
    14941 cctgaccata agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga
    15001 ttaacacgcg caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg
    15061 cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag
    15121 tagtataaat acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt
    15181 ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg
    15241 atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac
    15301 ggcagcggtg ccacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgc
    15361 agttatggcg aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt
    15421 attgcttccc tcaccaagct aatgaccagt gaaatgctgg tgaaattgct cgaccagggg
    15481 accgtgaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccaacc
    15541 tacaatggga cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt
    15601 gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc
    15661 tggaaatacc tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct
    15721 aaccttgcgt tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc
    15781 cagttgtttg aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc
    15841 tcaccggatc agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac
    15901 acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg
    15961 atgcagcagt atttatcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag
    16021 acgttgattt atcagcgcgc gcagtttacc aaagtgattg gcatggatgt ccccggcaaa
    16081 gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt
    16141 attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag
    16201 aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc
    16261 gatggcatca atgatctggt gaccgagcta agcgggaata aaccgttggt tatccctgca
    16321 tcctgattaa tactctgaag gcaaacagtt gatagcgacc agtttgcctt tattcatttc
    16381 gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg
    16441 tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc
    16501 aatcattgat aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac
    16561 gtcgcgtaat tccagaattc tatttttcca ggctaaccag taaaaagcgt ctcgccagag
    16621 ttgattttgt tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt
    16681 tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat
    16741 aatataagaa gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac
    16801 gccctctaaa ataaagaaag tatcttcgct attgacatca ctggaaaata taacttgctt
    16861 ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa
    16921 ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa
    16981 atccttgacg ggcattattt agattaaatt accagaattt cttcggagtg aagaatatta
    17041 ccaagtatat ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag
    17101 gtagttagca tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc
    17161 aacacgtacc gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc
    17221 gttgtttgag tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc
    17281 gctgtaggtg aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga
    17341 ctgaccgtca actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc
    17401 agaaacgctg ccgtaaggag tctcgtaacc agcatcaccc agtttgaagt cgtaaccggc
    17461 tttcaaaccg aagccccacg cgtcagagtt ggtgctaccg tcaacgtaag taccgttgct
    17521 catggttgca gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac
    17581 gttgttcgcg aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatccacctg
    17641 accagaacgg tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt
    17701 agcgttgtta ccgtcaattt tggtatcaac accgaccatg atgccgttaa catcctgatc
    17761 atagttgatg gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc
    17821 gccgttatca gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc
    17881 caggttccag atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt
    17941 cagctccatc tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc
    18001 acccaggtca gctttgttag cagcagagaa ggtcgcgttg gtgttagcgt cgtttacccg
    18061 aataagttct ttatctttgt aatcagcgat agtgccagcg ccagttgcgt tgtcgatacg
    18121 aactttgtag ttaccggttg cagcaacaac gttatcaaca gagctgttat ccagttcagt
    18181 accgttgtct acgtcgccgt taccattgat agtcaggtga ccatcagagt tcatagcaac
    18241 aataccgtaa ccgtagttag acttggaagt atcccacgta cggtcgttca ccagatcagc
    18301 gttcagaacg taatcgctgc tgttgatgtc gaacacacca gaaattacgt tgccattatg
    18361 gtcagcaatg ttgccgctgg tcagttccag tttatcagta cggaaagctt cagtatcgaa
    18421 ctgatcaaca ttcacatcca gcacaccacc gttagtcaca gtgatagtat tggtatacag
    18481 attagaataa ttattccaac cggtagattc ggttaaatta acggtactgt agctatcaac
    18541 agtcaggtgg tctgtagcaa ccacgccatt tgcaccaata ttcagtgaag aaccgttggt
    18601 taaaccaatg gtatctgata ccagagagga atcagtaaca tttacctgag agccattatt
    18661 tac
//
LOCUS       DAJVTY010000077        18179 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVTY010000077 DAJVTY010000000
VERSION     DAJVTY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18179
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.077"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..717
                     /locus_tag="OQQ66_002317"
     CDS             <1..717
                     /locus_tag="OQQ66_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1905228.1"
                     /translation="LYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(766..2421)
                     /gene="tsr"
                     /locus_tag="OQQ66_002318"
     CDS             complement(766..2421)
                     /gene="tsr"
                     /locus_tag="OQQ66_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1905229.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2540..2986)
                     /gene="hpaR"
                     /locus_tag="OQQ66_002319"
     CDS             complement(2540..2986)
                     /gene="hpaR"
                     /locus_tag="OQQ66_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1905230.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3258..4547
                     /locus_tag="OQQ66_002320"
     CDS             3258..4547
                     /locus_tag="OQQ66_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1905231.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4544..6003
                     /gene="hpaE"
                     /locus_tag="OQQ66_002321"
                     /pseudo
     CDS             4544..6003
                     /gene="hpaE"
                     /locus_tag="OQQ66_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
     gene            6005..6856
                     /gene="hpaD"
                     /locus_tag="OQQ66_002322"
     CDS             6005..6856
                     /gene="hpaD"
                     /locus_tag="OQQ66_002322"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1905232.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            6866..7246
                     /locus_tag="OQQ66_002323"
     CDS             6866..7246
                     /locus_tag="OQQ66_002323"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1905233.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7357..8160
                     /gene="hpaH"
                     /locus_tag="OQQ66_002324"
     CDS             7357..8160
                     /gene="hpaH"
                     /locus_tag="OQQ66_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1905234.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8171..8959
                     /gene="hpaI"
                     /locus_tag="OQQ66_002325"
     CDS             8171..8959
                     /gene="hpaI"
                     /locus_tag="OQQ66_002325"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1905235.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLSDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVHATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9134..10510
                     /gene="hpaX"
                     /locus_tag="OQQ66_002326"
     CDS             9134..10510
                     /gene="hpaX"
                     /locus_tag="OQQ66_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1905236.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10520..11406
                     /gene="hpaA"
                     /locus_tag="OQQ66_002327"
                     /pseudo
     CDS             10520..11406
                     /gene="hpaA"
                     /locus_tag="OQQ66_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11657..13219
                     /gene="hpaB"
                     /locus_tag="OQQ66_002328"
     CDS             11657..13219
                     /gene="hpaB"
                     /locus_tag="OQQ66_002328"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS1905237.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13237..13749
                     /locus_tag="OQQ66_002329"
     CDS             13237..13749
                     /locus_tag="OQQ66_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1905238.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14142..16292
                     /gene="btsT"
                     /locus_tag="OQQ66_002330"
     CDS             14142..16292
                     /gene="btsT"
                     /locus_tag="OQQ66_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1905239.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16423..16626
                     /locus_tag="OQQ66_002331"
     CDS             16423..16626
                     /locus_tag="OQQ66_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1905240.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16637..17593
                     /gene="yjiA"
                     /locus_tag="OQQ66_002332"
     CDS             16637..17593
                     /gene="yjiA"
                     /locus_tag="OQQ66_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1905241.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17695..>18179)
                     /locus_tag="OQQ66_002333"
     CDS             complement(17695..>18179)
                     /locus_tag="OQQ66_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1905242.1"
                     /translation="SVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWET
                     LSKRYGNNVSNWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPT
                     TSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES
                     "
BASE COUNT         4164 a         5039 c         4841 g         4135 t
ORIGIN      
        1 ctctatcgca accgcgagca cgtagaactg actgccgttg agcaagctta tctcaatgca
       61 ggtagcgtca atgcccgccg agatccgctc agttttgccg aatggcgcag cctgttccgt
      121 aaccgtacaa tgtggggaat gatgctcgga ttcagtggca tcaactacac tgcgtggctg
      181 tatctggcct ggcttcctgg ttacctgcaa acagcctata acctggattt aaaaagcaca
      241 gggttgatga cggctatccc tttcctgttt ggggctgccg ggatgctggt caacggttac
      301 gttaccgact ggctggtcaa agggggaatg gctccgatta aaagccgtaa gatctgcatt
      361 attgccggga tgttctgttc tgccgccttt acgctgatag taccacaagc gacaacatcc
      421 atgacggcgg ttctgctgat tggcatggca ctgttctgta ttcactttgc cggaacatcc
      481 tgccggggct tgatccacgt cgcagttgct tctcgcatga ctgcgtcggt gggcagtatc
      541 cagaactttg ccagcttcat ctgcgcctct tttgcgccga tcattactgg ttttattgtt
      601 gataccaccc actcattccg tctggcacta atcatctgcg gttgcgtcac cgcagcgggg
      661 gcactggcgt acatcttcct ggttcgtcag ccgatcaacg acccacgtaa agattaatct
      721 ccttatgccc gataacattt tgcttatcgg gcattttcat ggcgattaaa atgtttccca
      781 gttctcctcg ctatctgcca cggccatttt acgcggcgca gctggcgtca cggtttttac
      841 cacagccgat gtttcacgct gctgttgctg aatccggaac actgccactg cttcggtcag
      901 gcgactggcc tgctcttcca gcgcggcggc ggcagcggca gactcttcca ccagcgcggc
      961 gttctgttga gttacccggt ccatctcagc aaccgctaag ccaacctgat cgataccacg
     1021 gctctgctca tcagaagcag aagcaatttc gcccataatg tccgtcacgc gggtcacggc
     1081 gctgacaatc tccgccattg tttccccggc gctttcgacc agcgtagagc caacatccac
     1141 tttccccacc gagtcttcaa tcaggctttt aatttcacga gccgcctggg cgctgcgctg
     1201 ggccagatta cgcacttctc ccgcgaccac cgcaaaaccg cgcccttgct cacccgcacg
     1261 cgcagcctca accgccgcgt tcaaagccag aatattggtc tggaaggcaa tgccgtcaat
     1321 tacgctgata atatcggcga ttttctgcga actggtggag atatcgcgca tagtctgtac
     1381 cacgttatct accactttgc cgccgcgttg cgccgtttca gaagcactta acgccagatg
     1441 gctggcctgg cgcgcatttt cggcgttctg cttcaccgtt gcggtcagtt gctccatgct
     1501 ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga gatcgttatt
     1561 gccggtggcg atttcgctgg caccgctata gatggcattg gccccgttgc gcacatcacc
     1621 gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt gccccatctc
     1681 attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat gacgaatgct
     1741 gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc agacggcgaa
     1801 gatgaccgcc agtacgacga tcatcacgcc caccagaatc cacatcgcct ggctgtagga
     1861 ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca tgtaagccac
     1921 atactgcttc tcgaaaccgt cctgatatcc ctgggtcggc tgatcaaaga actcgttgat
     1981 tttgcctgca cctaacagtt ggatcagctc cgccagcgca ttgtgataaa tatcgtaatt
     2041 acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca acgcttcgta
     2101 atccgcccag tttttttccg cctgtttcag cgaaatactg gcactctgca tcagctcagc
     2161 aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac ccgcgcggtt
     2221 gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg attgctgctg
     2281 gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat taaagaacag
     2341 accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta agctggtcac
     2401 gatcttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaag attttcggct
     2461 tggaaagcaa aaactttatg aaaaggtgta acccgccatc catggaaaaa ccaggcgctg
     2521 gttatcagat actaaaaagt tattcattat cgcccgggat ggcttcttgt cgggaattgc
     2581 ccagagcaat aaattcctct aacagatggg ttaactgttg cattttctcg gcagtaaact
     2641 gagcttcaat ctgccgatag gcttcttcaa tctgtgtctg agcgcggtta tacagcgcct
     2701 gcccctcttt ggttagcgag atatagagct tccgctgatc attgatcggc tttaatcgca
     2761 acactaaacc gtcgcgttcc atccgcgtga gaatcccggt caggcttggg cgcaaaatgc
     2821 aggcgcgata cgccagatcg tgaaaatcca ttgatgggct ttccgccaga atacgcacga
     2881 tgcgccactg ttgctcagtc aaattatgcc gtttaacgat tgggcgaaaa taactcatcg
     2941 ccgcttctcg cgcctggagc aacgcaatgg ttagtgagtc gtgcattatc tttcccctga
     3001 aaaattcaaa atcattaata tagaaacagt taatcgtcac gttaccggga ctgcgctgcc
     3061 cctgcaattc ctgattttat caaaattgat gatattttat taacttactg atatttaata
     3121 ttttattatc tttaaatgta aatagtttgt taattagatc acatttacat cacttacttt
     3181 tgccaaacta cgcaatcggg aataaaagca atcattaaca tattaatgat taagataatc
     3241 gaatccgagg agtggtaatg aaaggcacta tcttcgccgt agcgttgaac catcgcagcc
     3301 aacttgatgc atggcaggaa gcgttccagc aatcccccta caaagccccg cctaaaactg
     3361 cggtctggtt tattaaaccg cgcaatacgg tgattggttg cggtgaaccg attccctttc
     3421 cacagggtga aaaggtactg agcggtgcga ctgttgcgct gattgtggga aaaacggcga
     3481 cgaaagtacg tgaagaagat gcggcagagt acatcgccgg atatgcgctg gctaacgacg
     3541 tcagcctgcc ggaagagagc ttttaccgcc cggcaatcaa agcaaaatgt cgtgatggat
     3601 tctgccccat tggcgaaacc gtggctctca gcaatgtcga taatctgacc atctataccg
     3661 agatcaacgg gcgtcctgcc gatcactgga ataccgccga tttacaacgt aacgccgcgc
     3721 agttgctgag cgccctgagc gaatttgcca cgctgaatcc aggcgacgcc attctgctcg
     3781 gcacgccaca ggcgcgcgtg gaaatacagc caggcgatcg cgttcgtgtt ctcgcagaag
     3841 gtttcccgcc gctggaaaat ccggtagtgg acgaacgtga agtgaccacg cgcaagagct
     3901 tcccaacgca gccacacccg cacggcacgc tgtttgccct cggcctgaac tacgccgacc
     3961 acgccagcga actggaattt aagccaccgg aagaaccgct ggtattcctg aaagcgccaa
     4021 ataccctcac tggcgataac cagacctccg tgcgtccaaa caatattgaa tacatgcact
     4081 atgaagcgga gctggtggta gttattggca aacaggcgcg taacgtcagc gaagccgatg
     4141 ccatggatta tgtcgcgggc tacaccgtgt gtaacgacta cgccattcgt gactatctgg
     4201 aaaactacta ccgccctaac ctgcgggtaa aaagccgcga cggactgacg ccgatgcttt
     4261 caaccatcgt gccgaaagag gcgatcccgg acccgcataa tctgaccctt cgcaccttcg
     4321 tcaacggcga gttacgccag caaggcacca ccgccgatct gatcttcagc gtgcccttcc
     4381 tgatcgccta cttaagtgaa tttatgaccc tgaatccggg cgacatgatc gccaccggca
     4441 cacccaaagg cttatctgac gtggtgcctg gcgatgaagt agtggtggaa gtagaaggcg
     4501 tgggccgcct ggtgaaccga attgtgagtg aggatacagc gaaatgaaaa aagtaaatca
     4561 ttggatcaac ggcaaaaatg ttgcaggtaa cgactacttc cagaccacca atccggcaac
     4621 gggtgaagtg ctggcggatg tggcctctgg cggtgaagcg gagatcaatc aggcggtagc
     4681 ggcagcgaaa gaggcgttcc cgaaatgggc caatctgccg atgaaagagc gtgcgcgcct
     4741 gatgcgccgt ctgggcgatc tgatcgacca gaacgtgcca gagatcgccg cgatggaaac
     4801 cgcggacacc ggcctgccga tccatcagac caaaaatgtg ttgatcccac gcgcttccca
     4861 caactttgaa tttttcgcgg aagtctgcca gcagatgaac ggcaagacct atccggttga
     4921 cgacaagatg ctcaactaca cgctggtgca gccggtgggc gtttgtgcgc tggtatcgcc
     4981 gtggaacgta ccgtttatga ccgccacatg gaaggtcgcg ccgtgtctgg cgctgggcaa
     5041 taccgcggta ctgaaaatgt cggaactctc cccgctgacc gcctgggtga gctggcgctg
     5101 gaagccggta ttccggcagg cgtgctgaac gtggtacagg gctacggcgc aaccgcaggg
     5161 gatgcgctgg ttcgtgatca tgacgtacgt gccgtgtcgt tcaccggcgg tacggccacc
     5221 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggaact gggcggtaaa
     5281 tcgccggtgc tgatttttga agatgccgat attgaacgcg cgctggacgc cgccctgttc
     5341 accatcttct cgatcaacgg cgaacgctgc accgccggtt cacgcatctt tattcagcaa
     5401 agcatctacc cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtcggc
     5461 gatccgaacg atccgaatac ccaggttggc gcgcttatca gccagcagca ctgggaaaaa
     5521 gtctccggct atatccgtct cggcattgaa gaaggcgcca ccctgctggc gggcggcccg
     5581 gataaaccgt ctgacctgcc tgcacacctg aaaggcggca acttcctgcg cccaacggtg
     5641 ctggcagacg ttgataaccg tatgcgagtc gcccaagaag agattttcgg gccggtcgcc
     5701 tgcctgctgc cgtttaaaga cgaagctgaa ggcttacgcc tggcaaacga cgtggagtac
     5761 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
     5821 attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg ccagccattt
     5881 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
     5941 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
     6001 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatctctc
     6061 tgaactgcca gggaaaaacc acggttgccg ccagggcgcg atcgacgggc ataaagagat
     6121 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata cccactggct
     6181 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
     6241 cgagctgccg cattttatcc gcgatatgac ctacaactac gagggcaacc cggagttggg
     6301 gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc acaacattcc
     6361 cagcctgaaa ctggaatacg gcacgctggt gccgatgcgc tacatgaatg aagacaagca
     6421 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
     6481 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
     6541 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcagaagaag ggatgaacag
     6601 ctacacccgc gagttcgacc gccagatgga cgagcgcgtg gtgaagttgt ggcgcgaagg
     6661 ccagttcaaa gagttctgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
     6721 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
     6781 ggtggagttt atcaccgagc tattcccaag ctctggcacc ggtcaggtta acgctgtttt
     6841 cccgcttccc gcgtaaggac gttttatgcc gcactttatc gttgaatgca gtgacaacat
     6901 ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg aatccgacgc tggcagccac
     6961 gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata cctggcagat
     7021 ggccgacggg cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg gcgcaggtcg
     7081 cagcctggaa agccgtcagc aggcgggtga aatgctgttt gaactgatta aaacgcactt
     7141 cgccgccctg atggagagcc gcctgctggc gttgtcgttt gagattgaag agctgcatcc
     7201 gacgctgaat tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg cagattgccc
     7261 ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag gccggataag
     7321 gcgcagccgc atccggcaat gccacaggat atcgctatgt tcgataaaca cacccacacc
     7381 ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg aacagatccg cgcgatctcg
     7441 ctggattacc cagagatcac catcgaagac gcttacgcgg tgcagcgcga atgggttcgc
     7501 ctgaaaatcg ccgaaggccg tacgctgaaa ggccacaaaa tcggtctgac ctcaaaagca
     7561 atgcaggcca gctcgcagat cagcgaacca gattacggtg ccctgctgga tgacatgttc
     7621 ttccacgatg gcagcgatat cccgaccgat cgctttatcg tgccgcgcat tgaagtggag
     7681 ctggctttcg tgctggcaaa accgctgcgt gggccaaact gcacgctgtt cgacgtctac
     7741 aacgccacgg actacgtgat cccggcgctg gagctgatcg acgctcgctg ccacaacatc
     7801 gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca tttctgataa cgccgccaac
     7861 gccggggtga tcctcggtgg tcgtccgatc aaaccagacg aactggatct gcgttggatc
     7921 tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc tggcgtgctg
     7981 aatcatccgg caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta tgacgtacaa
     8041 ctggaagccg ggcaaatcat tctcggtggt tcgttcaccc gcccggttcc ggcgcgtaag
     8101 ggcgacacct tccacgtcga ttacggcaac atgggttcca ttagctgccg ctttgtttaa
     8161 ggagagaacg atggaaaaca gctttaaagc ggcgctgaaa gcaggccgtc cgcagatcgg
     8221 attatggctg gggctgagta gcagctacag cgcagagtta ctggccggag caggattcga
     8281 ctggttgttg agcgacggtg agcacgcgcc gaataacgtg caaaccgtgc tcacccagct
     8341 acaggcgatt gcgccctacc ccagccagcc ggtagtacgt ccgtcgtgga acgatccggt
     8401 gcaaatcaaa caactgctgg acgtcggcac acaaaccttg ctggtgccga tggtacaaaa
     8461 cgctgacgaa gcccgtgaag cggtacacgc cacccgttat ccccccgccg gtattcgcgg
     8521 tgtgggcagt gcgctggccc gcgcctcgcg ctggaatcgt attcctgatt acctgcaaaa
     8581 agccaacgat caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa tgaagaactt
     8641 accgcagatt ctggacgtgg aaggcgtcga cggcgtgttt atcggcccgg cggatctgag
     8701 cgccgatatg ggttatgccg gtaatccgca gcacccggaa gtacaggccg ccattgagca
     8761 ggcgatcgtg cagatccgtg aatcgggcaa agcgccgggg atcctgatcg ccaatgagca
     8821 actggcaaaa cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg ttgacaccac
     8881 cctgctcgcc cgcgccgctg aagcgctggc agcacggttt ggtgcgcagg ccaccgccgt
     8941 gaagcccggc gtgtattaat gccggatgcg ctgcacttat ccggcctaca ctcgcaccaa
     9001 accgtaggcc ggataaggcg tttacgccgc atccggcaaa aagccgtacc aaatcgcgga
     9061 ccggataagg cgttacgccg catccgccat aaaccttgtc gtaccctaca aaaatcccat
     9121 tagaggaaga aaaatgagcg acacctcacc tgccataccg gagagtatcg atccggcgaa
     9181 tcagcataaa gcgctgactg ccggacaaca ggcggttatt aagaagctat ttcgccgcct
     9241 gatcgtcttt ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca acatcggctt
     9301 tgccggactc acgatgggac gcgacctcgg tctgagcgcc accatgtttg gcctcgctac
     9361 caccctgttc tatgccgctt atgtcatctt cggcattccc agcaacatta tgctgagtat
     9421 tgtcggtgcg cggcgctgga tcgccaccat catggtgctc tggggcatcg cctctactgc
     9481 caccatgttt gccactggcc ccaccagctt gtacgtactg cgtatactgg ttggcattac
     9541 cgaagccggc tttctgcctg gtattctgct gtatttaacc ttctggtttc cggcctattt
     9601 ccgcgcccgt gccaacgcct tgtttatggt agcaatgccg gtaacgacag cgttgggctc
     9661 gatcgtttcc ggctacattt tgtcgctgga tggcgtcatg gcattaaaag gctggcagtg
     9721 gctgtttttg ctggaaggct tcccgtcggt attactcggc gtcatggtgt ggttctggct
     9781 tgatgactca ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat gcctgcaaga
     9841 gatgatggat aacgatcgtc tgacgctggt tcagccagag ggagccatca gccaccacgc
     9901 catgcaacaa cgcagtatgt ggcgggagat cttcactccg gtggtgatga tgtataccct
     9961 ggcgtatttc tgcctgacca acacacttag tgcgatcagc atctggacac cgcagatcct
    10021 gcaaagtttt aatcagggca gcagtaatat caccatcggc ctgctggccg ccgtaccgca
    10081 gatttgtacc attctcggga tggtctactg gagccgtcac tcagatcgcc gccaggaacg
    10141 aaggcatcac accgcccttc cttatttgtt cgctgccgca ggttggttac tggcttcggc
    10201 aactgatcac aacatgatcc agatgctggg gatcattatg gcttcgaccg gatcattcag
    10261 cgcaatggcg attttctgga caacaccgga tcagtccatc agcctgcggg cacgagcgat
    10321 cggtattgcg gtgatcaacg ccactggcaa cattggttca gcgttaagtc cgtttatgat
    10381 cggctggttg aaagatttga ccggcagctt taacagtgga ttgtggtttg ttgccgcgct
    10441 gctggtgatt ggtgcgggga ttatctgggc gattccaatg cagtcctccc gtccgcgagc
    10501 gaccccgtaa ggaacgacga tgtgtgaccg tcagattgcc aatattgata tcagcaaaga
    10561 gtacgatgaa agcctgggca cggacgatgt gcattatcag tcgttcgccc gcatggcggc
    10621 attttttggc cgccatatgc tgccacatcg ccacgaacag tactttcaga tgcatttcct
    10681 caatagcgga cagattgagc tacagcttga cgatcatcgc tactcggtgg aagcgcccct
    10741 gtttgtcctg acgccgccgt cagtacctca tgcgtttatt acggagtctg acgccgacgg
    10801 tcatgtgttg acggtacggg aagatctgat ctggcccctg ctggaagttc tttatccggg
    10861 cactcgggaa accttcggcc tgccgggggc ctgtcactgg cagataaacc cgacgaactg
    10921 gcggcgctgg aacactattg gcaactgata gagcgggaat cggtggaaca actgcctgga
    10981 cgggaacaca ccctgacgtt actggcacag gcagtgttca ccctactgct gcgtaacgca
    11041 aaactcgacg accatgccgc cagcgggatg cgcggagaat taaaactgtt ccagcgtttt
    11101 aatatgctga ttgaaagcca ttttcatcag cactggacag taccggatta cgctaacgaa
    11161 ctgcatatca ccgaatcacg actcacggac atctgccgtc gctttgccaa ccgtccgcca
    11221 aaacggttga ttttcgacag gcagctgcga gaagccaagc ggctgctgct gttttctgat
    11281 aacgccgtga acaatattgc ctggcaactc ggttttaagg atccggctta ttttgcgcgc
    11341 ttttttaatc gcttagtcgg ttgctcgccc agtgcttatc gcgccaaaaa agtacctgtg
    11401 acgtgaaaat cccttagcct taacgggaaa ccaggcacca cctgctattc ccctttctcc
    11461 gcctggagca aggggattta gtaacgtcaa aaatcgctaa aagcgacctc gatcacaaaa
    11521 ccgtacccga cctgaaaagt accagccatt gtcccaaaag tctcttcccg taatcgcccc
    11581 tgcaagcttt aattgaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa
    11641 tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtccgttcac
    11701 cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg
    11761 agtgaaagac gtcactactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact
    11821 gtacgacgcg ctacacaaac cggagatgca ggactctctg tgctggaaca ccgacaccgg
    11881 cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccaacg acctgcgcca
    11941 gcaacgcgat gccatcgctg agtggtcacg cctgagctat ggctggatgg gccgtacccc
    12001 agactacaaa gctgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt
    12061 cgagcagaac gcccgtaact ggtacacccg tattcaggaa actggcctct actttaacca
    12121 cgcgattgtt aacccaccga tcgatcgtca tttgccgacc gataaagtaa aagacgttta
    12181 catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga aagtggttgc
    12241 caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac aagtgatggg
    12301 cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg gcgtaaaatt
    12361 aatctctcgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat acgactaccc
    12421 gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg tgctgatccc
    12481 atgggaaaac gtactgatct accgcgattt cgatcgctgc cgtcgctgga caatggaagg
    12541 cggttttgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga aattagactt
    12601 cattacagca ctgctgaaaa aatcactcga atgtaccggc accctggagt tccgtggtgt
    12661 acaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat tgagtgactc
    12721 gatgtgttct gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc atgccgcact
    12781 gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca ttatcgaacg
    12841 caacgttacc agtggcctga tctatctccc ttccagtgcc cgtgacctga acaatccgca
    12901 gatcgaccag tatctggcga agtatgtgcg cggttcgaat ggtatggatc acgtccagca
    12961 catcaagatc ctcaaactga tgtgggacgc cattggcagc gagtttggtg gtcgccacga
    13021 actgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcagt gtctgcgcca
    13081 ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct gcctgtcgga
    13141 atacgaccag aacggctgga ctgtgccgca cttgcacaac aacgacgata tcaacatgct
    13201 ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga acagcgcctg
    13261 cgctttcgtg acgcgatggc cagcctgtcg gcagcggtaa atattatcac caccgagggc
    13321 gacgccggac aatgcgggat tacggcaacg gccgtctgct cggtcacgga tacaccacca
    13381 tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca gggcaacggt
    13441 aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcacgcca cttcgcgggc
    13501 atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa aggtccgctg
    13561 gcgcagccgg tgctgaaagg ttcgctggcc agtcttgaag gtgagatccg cgatgtgcag
    13621 gcaattggca cacatctggt gtatctggtg gagattaaaa acatcatcct cagtgcagaa
    13681 ggtcacggac ttatctactt taaacgccgt ttccatccag tgatgctgga aatggaagct
    13741 gcgatttaag ctccagcccc ttcacgtatt cgtcgaaggg gcatttttat tgtgatctca
    13801 atcacatatc cacccgatga ataattcttg cccaaccgct caccgccatt cctgaccgct
    13861 tacgtcccta aaataaccac tcagttattt accttacttt acgcgcgcgt aactctggca
    13921 acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc
    13981 ttcggcctat gattaacaaa ttactctatg aattttggtt tgcaggaagg cagcaagtga
    14041 gtgaatcccc gggagcttac aacagtaagt gacagaggtg aacgaacgca gctgccgcac
    14101 ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg aaaaaactat
    14161 tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc gcggtagttg
    14221 cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct gtgtcggtgt
    14281 atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg aaactcgacc
    14341 ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt ccgaccaacc
    14401 gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgccggtccg ctggttggtc
    14461 cggtgctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg gcgggggtag
    14521 tactggccgg tgcggttcag gactttatgg tgctgtttat ctcctctcgc cgtaacggcg
    14581 catctctcgg tgagatgatc aaagaagaga tgggaccagt accggggact atcgcgctgt
    14641 ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt gtggttaaag
    14701 ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt
    14761 ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt aggtgaagtt tctgttattg
    14821 gtatcgtgct gctggttgcc tctatctatt tcggtggcgt gattgctcac gatccgtact
    14881 ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt ggctatgcgt
    14941 ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat ctggcaacct
    15001 tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtactg aacccggaac
    15061 tgaaaatgcc tgcaatgacc cagtacattg acggtactgg cccgctgtgg aaaggcgctc
    15121 tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc cacgcgctga
    15181 tctcttccgg tacgacgccg aaattgctgg ctaacgaaac cgacgcgcgt ttcattggct
    15241 acggcgcaat gctaatggaa tccttcgtgg cgattatggc gctggttgct gcgtccatca
    15301 tcgaaccggg tctttacttc gcgatgaata ccccgcctgc tggccttggc atcaccatgc
    15361 ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag ctgaaagacg
    15421 ttaccgcaca cgcggcggcg accgtcagct cctggggctt cgtgatttcg ccagagcaga
    15481 tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca ggtggcgcgc
    15541 cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg gctgacatgg
    15601 gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc gcgctggatg
    15661 cgggtactcg ttctggccgc tttatgctgc aagacctgct gggtaacttc atcccgttcc
    15721 tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt gtgggtctgt
    15781 ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag agcctgtggc
    15841 cgctgttcgg tatttccaac cagatgctgg cagccgtagc gctggtactg ggcaccgttg
    15901 tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg gctgtatggc
    15961 tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac ccgcagatgg
    16021 aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt actgacctga
    16081 cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc aacgcaggcc
    16141 tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc aaaacctggc
    16201 ttgcggtgcg taacagcgac aaacgtactg acaaagaaac tccgtacgtt ccaatcccgg
    16261 aaggcggcgt gaagatctct tcgcaccact aaccggttgc cggatgcgct gcgcgtatcc
    16321 ggcctacaaa gtgtctatcg taggccggat aaggcgttta cgccgcatcc ggcaaatccc
    16381 tagcccggac cttccgggct ttacgcatat ctggaaggca aaatgtttgg taacttagga
    16441 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggcattcc agactacgac
    16501 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
    16561 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
    16621 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
    16681 gggcaaaacc accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
    16741 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
    16801 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggaaga
    16861 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg accgtctggt
    16921 cattgaatgc accggcatgg ccgatcccgg cccgattatt cagaccttct tctcccatga
    16981 gattttatgc cagcgctacc tgctggacgg ggtgattgcg ctggtggatg cggtacatgc
    17041 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gttggctacg ccgaccgtat
    17101 tctgctgacg aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg
    17161 catcaacgcc cgcgcaccgg tctacaccgt tacccacggc gatatcgacc tgggccttct
    17221 gttcaacacc aacggtttta tgctggaaga aaacgtcgtc agcaccaaac cgcgtttcca
    17281 ctttatcgcg gacaaacaaa acgatatttc gtcgattgtg gtggaactgg attacccggt
    17341 agatatcagc gaagtttccc gggtgatgga aaacctgctg ctggaatcgg cggataaact
    17401 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
    17461 cgtccagcgc ctctacagcg ccgactggga ccgcccgtgg ggcgatgaaa aaccgcatag
    17521 cacgatggtg tttatcggga ttcagttgcc ggaggatgag attcgggcgg cgtttgcggg
    17581 actcaaaaag taaccagccc tccaacatca caatacctga gactttatca ccctcatatt
    17641 cctccagaat atgagggcat tcgggcttaa ttatctctga acgtgcaaca cttcctacga
    17701 ctccttatgc gcctccacat cctgcttcgt taaccagagc gacttacgac caaaattttc
    17761 gtacattttc agctgatctt catagtgctg atcaactgtt ccatcgggag caataaaccc
    17821 actctgaccg ggtgcgacca catcccaggc aagcaccgga cgatcgcttg tcgttggtga
    17881 gaaaacaatc atatcgtttt ctgttccacg gttttgatac tccgcctgat gacgcgtttc
    17941 ttccgctgcg gcctgcggta catcaaagaa attatttgcc cggaacgtta aggccattgc
    18001 aggtgttttc cagttactca cattattgcc atagcgtttg gaaagagtct cccaggtatc
    18061 ttccagcgca gccaacacaa cctcctgctg tggtttccca gcaaacagat caaccgcctg
    18121 tgggattggt gatttgtctc cctgcaccgc ctcatacaaa atttttgctc caacactta
//
LOCUS       DAJVTY010000078        17989 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVTY010000078 DAJVTY010000000
VERSION     DAJVTY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.078"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..88)
                     /locus_tag="OQQ66_002334"
     CDS             complement(<1..88)
                     /locus_tag="OQQ66_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HCS1905243.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEAR"
     gene            complement(93..278)
                     /locus_tag="OQQ66_002335"
     CDS             complement(93..278)
                     /locus_tag="OQQ66_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1905244.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(275..2914)
                     /locus_tag="OQQ66_002336"
     CDS             complement(275..2914)
                     /locus_tag="OQQ66_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1905245.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3122..4111
                     /gene="ldhA"
                     /locus_tag="OQQ66_002337"
     CDS             3122..4111
                     /gene="ldhA"
                     /locus_tag="OQQ66_002337"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1905246.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4222..4644
                     /gene="hslJ"
                     /locus_tag="OQQ66_002338"
     CDS             4222..4644
                     /gene="hslJ"
                     /locus_tag="OQQ66_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1905247.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4641..4907)
                     /locus_tag="OQQ66_002339"
     CDS             complement(4641..4907)
                     /locus_tag="OQQ66_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1905248.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5181..8705
                     /gene="nifJ"
                     /locus_tag="OQQ66_002340"
     CDS             5181..8705
                     /gene="nifJ"
                     /locus_tag="OQQ66_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1905249.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9071..10204
                     /gene="ompN"
                     /locus_tag="OQQ66_002341"
     CDS             9071..10204
                     /gene="ompN"
                     /locus_tag="OQQ66_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCS1905250.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10345..10779
                     /gene="uspF"
                     /locus_tag="OQQ66_002342"
     CDS             10345..10779
                     /gene="uspF"
                     /locus_tag="OQQ66_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1905251.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11336..12250
                     /gene="sitA"
                     /locus_tag="OQQ66_002343"
     CDS             11336..12250
                     /gene="sitA"
                     /locus_tag="OQQ66_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1905252.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12250..13077
                     /gene="sitB"
                     /locus_tag="OQQ66_002344"
     CDS             12250..13077
                     /gene="sitB"
                     /locus_tag="OQQ66_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1905253.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13074..13931
                     /gene="sitC"
                     /locus_tag="OQQ66_002345"
     CDS             13074..13931
                     /gene="sitC"
                     /locus_tag="OQQ66_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1905254.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            13928..14785
                     /gene="sitD"
                     /locus_tag="OQQ66_002346"
     CDS             13928..14785
                     /gene="sitD"
                     /locus_tag="OQQ66_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1905255.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15113..15886)
                     /locus_tag="OQQ66_002347"
                     /pseudo
     CDS             complement(15113..15886)
                     /locus_tag="OQQ66_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
     gene            complement(15892..16404)
                     /locus_tag="OQQ66_002348"
                     /pseudo
     CDS             complement(15892..16404)
                     /locus_tag="OQQ66_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
     gene            complement(16443..16730)
                     /locus_tag="OQQ66_002349"
     CDS             complement(16443..16730)
                     /locus_tag="OQQ66_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905256.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(16980..17135)
                     /gene="hokD"
                     /locus_tag="OQQ66_002350"
     CDS             complement(16980..17135)
                     /gene="hokD"
                     /locus_tag="OQQ66_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1905257.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(17363..>17989)
                     /locus_tag="OQQ66_002351"
     CDS             complement(17363..>17989)
                     /locus_tag="OQQ66_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1905258.1"
                     /translation="PNGANKLLLHSCCAPCSGEVMEALQASGIDYTIFFYNPNIHPQK
                     EYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGIRCTMCFDMRFERT
                     ALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWDYNWRKQGGSSRMI
                     EISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKEEKE"
BASE COUNT         4480 a         4586 c         4324 g         4599 t
ORIGIN      
        1 ttctggcttc atccagagtc aatgccatta cattgctgct taccagccca acaagaaacg
       61 cacaaagaag taatgttttc ttcatcatcg cctcagtaaa gatcgctacg agtttcaagc
      121 agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt aacgttcata
      181 ttgatagtga tcggttcctt gggcgcagcg acttcaatac gcggcgtaca ggcagccagc
      241 ataaaagatg acgtcaacgc agccagtaaa attttcattg tttttcctca cactcttttc
      301 cttgcggaca gtcatttccc ggcagacgtg cgttctgctc caaccatgcc tggagattat
      361 cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg taattaaggt
      421 ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatgttggcc tgcatggtta
      481 ataaaccgag attatctaaa ttaatatttg tcgatgaacg gctaatttcc atatagcgca
      541 accagttaat tgctgaaccc gcagtcatat tgtctttcac caccgcatct gcggtgtctt
      601 tatccagccg caaggtcatt ggcccgctat ttgccagcca accatctttc actatccatt
      661 tttcgttgtt tagccataac ggcaatgcac cactaaatgc cccggacatg gcgaattgtt
      721 tcggattgac ggcgctaacc agttcgctgg atgagagatt attcagccgc aacagcgccg
      781 ggtcatgttg cggcatacgt aattgtttca tcagtacgtt accgcctaac acatcgacgc
      841 taacatcagt caacagcaag ggttcttctt cagtccacgg ataacgccct tgcaaatcag
      901 ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac gtaacggggc
      961 cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca aaatcgacac
     1021 cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg tgccctcccg
     1081 cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt tcaccatcgc
     1141 gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt gattgttttg
     1201 gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc cgaaccgggc
     1261 caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg cgcccatcga
     1321 cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg
     1381 cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg
     1441 gcttgtccag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt aactggtcga
     1501 aaccggtgga gaggtcggtc agcgtaatcg tgctgtcatg ccattcacct tttcctgcga
     1561 catcccaggt ggcattcatc ggtgtaaaac tgccttttcc ccagtagcgc cactgccagc
     1621 ggcccgcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg aaatcgccca
     1681 gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca cgttgggtga
     1741 cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg atgactcttc
     1801 cctttgaacg aagtaacgcg cctggctcaa aggccagcgt tgggtcagtc agacttccac
     1861 ttagctgcgc gggtaagcgg gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca
     1921 gctgcagagg cagctggctg ttatccatgc ttaattttcc tgggccaaaa tttagcaccg
     1981 cgttgccttt acccgcttgc ccctgggtca gcacactcag tcgtccgctg atcagagcgt
     2041 tctcaagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc aaaggaaaac
     2101 ctgcatacgg ccaactccag cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc
     2161 acggcaaatc gagcaacgga tcgccgttat cacgtgccag cacaatcaat tgcccgctat
     2221 tttcctgcca gtccagctct gcatccacca gtgacggttc ctgcggcaag tttaacgtcg
     2281 cagtagcatg cccacttaca ggaagtccat ccggcacgag cggcatagta aattccccca
     2341 ccagtttaac cggcggctga tattcaaacg cgatgacatc cagttcgctg actgtaagtt
     2401 gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc agttgctgga
     2461 tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccaggga gaaaaaatca
     2521 gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg agggtttttg
     2581 gagcagctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa tcaagttcta
     2641 ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg gtgatatgtg
     2701 caagctgaca atctcccacc agataacgga gatcgggaat gattaaacct ttacgcgtaa
     2761 tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg cccgccagcg
     2821 tgggaaccca cagcccgagc gtcatcagca gcgtcaacgg cacaagaata atcagtaata
     2881 acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct gatttagcga
     2941 aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg aaggttgcgc
     3001 ctacactaag catagttgtt gatgaatttt tcaatatcgc catggttttc aattatattt
     3061 gaaattttgt aaaatatttt tagtggctta aatgtgattc aacatcactg gagaaagtct
     3121 tatgaaactc gccgtttata gcacaaaaca gtacgacaag aagtacctgc aacaggtgaa
     3181 cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa aaaccgctaa
     3241 aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca gccgcccggt
     3301 gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg ccggtttcaa
     3361 taacgtcgac cttgatgcgg caaaagaact ggggctgaaa gtagtccgtg ttccagccta
     3421 tgatccagag gccgttgctg aacacgccat cggtatgatg atgacgctga accgccgtat
     3481 tcaccgcgcg tatcagcgta cccgtgacgc taacttctct ctggaaggtc tgaccggctt
     3541 tactatgtat ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg tggcgatgct
     3601 gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc caagtgcagc
     3661 ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg aatcagacgt
     3721 tatctctctg cactgcccgc tgacaccggc aaactaccat ctgttgaacg aagccgcctt
     3781 cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat tgattgattc
     3841 tcaggcagca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta tggacgtgta
     3901 tgagaacgaa cgcgatctgt tctttgaaga taaatccaac gacgtgatcc aggatgacgt
     3961 attccgtcgc ctgtctgcct gccacaacgt gctgtttacc gggcaccagg cattcctgac
     4021 agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca atctggaaaa
     4081 aggcgaaacc tgcccgaacg aactggttta atgttgcagc tcccctgcaa tccaggggag
     4141 ctgattcaga taatccccaa tgacctttca ttctctattc ttaaaatagt cctgagtcag
     4201 aaactgtaat tgagaaccac aatgaagaaa gtagccgcgt ttgttgcgct aagcctgctg
     4261 atggcgggat gtgtaagtaa tgacaaaatt gctgttacgc cagaacagct acagcatcat
     4321 cgctttgtgc tggaaagcgt aaacggtaag cccgtgacca gcgataaaaa tccgccagaa
     4381 atcagttttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa
     4441 ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc
     4501 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     4561 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     4621 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     4681 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtgccatc cagttgacgg
     4741 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     4801 gcttttaaac ctggcgctac gtgcgccgca gtcgcctgct gaacaggttc actactacac
     4861 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctt ttcttcctct
     4921 gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc gacatcgaga
     4981 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     5041 tgatcaaaac attcattacg ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     5101 catttgctaa aattgaaaga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     5161 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     5221 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     5281 aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg
     5341 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg
     5401 gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacactgt
     5461 acaaactggc aggcgaactc acaccgtttg tcctgcatgt agcggcacgt accgttgcca
     5521 cacatgcact ctctattttt ggcgatcatt ccgacgtcat ggcggtgcgc cagacgggtt
     5581 gcgcgatgtt gtgtgcggca aacgtccagg aagcgcaaga ctttgctctc atttcgcaca
     5641 tcgcgacgct gaaaagccgc gtgccattta ttcatttctt tgatggtttc cgcacgtccc
     5701 acgaaatcaa taaaattgtc ccgctggccg atgacacgat tcttgatctc atgccgcagg
     5761 ccgaaattga tgctcatcgc gcccgggcgc tcaacccgga acatccggtg atccgtggta
     5821 cgtccgccaa tcctgactct tatttccagt ctcgcgaagc caccaacccc tggtacaacg
     5881 cagtctatga ccatgttgaa caggcgatga atgatttctc tgccgcgaca ggtcgtcagt
     5941 atcagccgtt tgaatattac gggcatccgc aagctgaacg ggtgattatc ctgatgggct
     6001 cagccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagtcg
     6061 gcgtgctgaa agttcgcctg taccgcccct tctcagccaa acacttgcta caaactctac
     6121 cgggatccgt acgcagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag
     6181 aaccgctcta tctggatgtg atgaccgcac tggcagaagc ctttaataat ggcaagcgcg
     6241 aaactctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     6301 actgtgtact ggcggtattt gccgagctca acgcggctaa accgaaagcg cgctttacgg
     6361 ttggtattta cgatgatgta accaatctgt ccctgccgtt gccggaaaac acccagccaa
     6421 actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcga
     6481 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     6541 tttacgactc caaaaaagcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     6601 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     6661 ttatcgataa atatcagatg gctgagcgtt taaaacctgg cggcattttc ctgctcaaca
     6721 cgccgtacag cgcagatgaa gtgtggtcgc gcttgccgca agaagttcag gccgtgttaa
     6781 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     6841 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc
     6901 ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     6961 gccaggatct ggtggaaggc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     7021 aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg
     7081 ccgcccctga tttcgtgaaa accgtaaccg cagcgatgct cgcagggctt ggtgatgccc
     7141 tccccgtttc ggcgctgccg ccagacggca cctggccgat ggggaccact cgctgggaaa
     7201 aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc
     7261 actgcgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtacca cccgaagcga
     7321 tggaaaacgc ccctgccagc ctgcattcgc tggatgtgaa atcgcgtgat atgcgcgggc
     7381 agaaatatgt cttgcaggtg gctccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     7441 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtcgcgcc
     7501 tggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     7561 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     7621 aatattccgg tgcttgctcc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     7681 tctatggcga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt tatggcggta
     7741 acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact
     7801 ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     7861 gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga
     7921 cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc
     7981 agcaactcaa cgatgttgcc gaagcacatg aactgctacg tgatgcagat gcactggtgg
     8041 aaaaatcaat ctggctgatt ggtggtgatg gctgggctta cgatatcggc tttggcggtc
     8101 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     8161 attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaaatttg
     8221 gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     8281 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa tcagacggtg aaagcgattc
     8341 aggaagcgga agcgtatccg ggaccatcgc tgatcattgc ttatagcccg tgtgaagagc
     8401 atggttacga tctggcactc agccatgacc agatgcgcca actcacagct accggcttct
     8461 ggccgctata tcgcttcgat cctcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     8521 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcaa cgtttccgtc
     8581 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     8641 tgcaaaaacg ctatgacttc ctggcacaaa tggccggaaa agcggaaaaa agcaacaccg
     8701 attaaatgct ctggataagg attatccaat tctaaaaaaa agcccggacg actgttcggg
     8761 cttgtctttt tatatgttgg aaaatcagtg gcaatgcaat ggcccaacag aaaataaaat
     8821 ctgtgacaat aaaggcatat aacccgcgca gaataacgta tagaaaatta aaagtataat
     8881 ttttattttg tatgtattat cttcgataca ttcaccagac ttattatcat ttcagaagaa
     8941 ttatttgatt aaggttttac ttaaggcgta acaaatgata tttatcggct aactgaactt
     9001 ctcctttagg atgttttcac tccccggaat ggggataaaa ggcaaataaa ataactaaag
     9061 gatttattca atgaaaagca aagtactggc acttttaatt cctgccctgc tcgccgcagg
     9121 tgctgcacat gcagccgaag tttataataa agacggcaac aaattagatc tgtatggcaa
     9181 agttgatggc ctgcattatt tttctgataa ttcagcgaaa gatggcgatc agagctatgc
     9241 gcgtctgggt tttaaaggcg aaacccaaat taacgatcaa ctcactggct acggtcaatg
     9301 ggaatacaat attcaagcaa acaacactga atcttcaaaa aaccagtcat ggacccgtct
     9361 ggcatttgcc gggctgaaat ttgcagatta cggttctttc gattacggac gtaattatgg
     9421 cgtaatgtac gacatcgaag gctggaccga tatgctgcct gaatttggcg gtgactctta
     9481 taccaatgca gacaacttta tgactggtcg agccaatggc gtcgcgactt atcgtaatac
     9541 tgatttcttc ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag gtaacaacga
     9601 aggagccagt aatggtcagg aaggcaccaa caacggacgt gatgttcgcc atgaaaacgg
     9661 tgacggctgg ggtttttcca caacatatga tttaggcatg ggctttagcg ctggtgcggc
     9721 atacacctct tctgaccgca ccaatgacca ggttaaccat actgcggcgg gtggtgataa
     9781 agcagacgcg tggactgctg ggctaaaata cgatgctaac aatatttacc tggcaaccat
     9841 gtattcagaa acgcgtaata tgaccccgtt tggcgacagc gattatgctg tggcaaacaa
     9901 aacccagaat tttgaagtca ctgcccagta ccagtttgat tttggtctgc gtccggcagt
     9961 ctctttcctg atgtctaaag gccgtgacct gcacgctgcc ggtggtgcag acaacccggc
    10021 aggtgttgat gataaagatc tggttaaata cgccgatgtt ggcgcgactt actatttcaa
    10081 taaaaacatg tccacctacg ttgactataa aatcaacctg ttggatgaag atgacagctt
    10141 ctacgctgcc aatggcatct ctaccgatga tattgtcgct ttaggtctgg tttatcagtt
    10201 ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc ttattcttcc tcctttgatc
    10261 taaattaaaa atgtgaactc catcattaca caaaaagtgt catctggcgt tacactttat
    10321 gcggatacta aaacaggagg ttttatgaac agaacgattc ttgtccctat cgatatttcc
    10381 gattcagaat taactcaacg cgtgattagc cacgttgagg cagaggcaaa gattgacgat
    10441 gcagaggttc atttcctgac ggtaatacct tcactgccct actatgcctc tctgggttta
    10501 gcgtattccg cagaattacc ggcaatggat gacctgaaag cggaagccaa atcgcaactg
    10561 gaagagatca ttaaaaaatt taaactgcca accgacagag tgcatgtcca tgttgaggaa
    10621 ggctcgccca aagaccgcat tctggaattg gcgaagaaga tccccgctca tatgatcatc
    10681 attgcttccc atcgaccgga tatcaccact tatctgctcg gttccaacgc cgcagctgta
    10741 gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac actaacgccc gcacattgct
    10801 gcgggctttt tgattcattt cgcaaatgtg ctgacatttt cccctctaat ccgtaccata
    10861 cacgccactg tttttatatc agatttctta tgctgggcat tccggcatga cgcatactct
    10921 tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa
    10981 gaaagaacaa tacaacctga acaagtaagg gcaaaaatca caactatctg actcataagt
    11041 attttattta tttttcaata tgttaaacat tatcagcgac aacaataagc tacgataacc
    11101 cactcttttt ttgccccatt tttgccatca acaattttcc tcataaagtc atttcaaagc
    11161 ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac aacctaatcc
    11221 ctgttttagg cctcaaagct gttaatgata attattttca ttttgtatag gttatgctat
    11281 aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact aaattatgca
    11341 ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct cgatcgcatt
    11401 tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca tcgcggatat
    11461 ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc ctggtgcaga
    11521 aattcatgag tatcagccta cccctggcga tattaaacgt gcacaggggg cacaactgat
    11581 tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc atctcaatgg
    11641 ggttccagaa gtaattgtct cttcgggtgt gacgccagta gggatcaccg aaggacccta
    11701 tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga tttacgtcga
    11761 taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct accaacgtaa
    11821 tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta agcagattac
    11881 ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt cttatctcgc
    11941 acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc aacaaggaac
    12001 accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc cggcagtctt
    12061 tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa ccggcgcaca
    12121 ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg tacctacgta
    12181 tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag ccgggaaaag
    12241 ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt cacctggcgt
    12301 aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc tattgccgct
    12361 ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat gggatttgtg
    12421 cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc gctacagaaa
    12481 aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc tgtactggtg
    12541 gaagatgtgg tgatgatggg ccgctatggg tatatgggta tgttgcgtat agccaaaaag
    12601 agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga ttttcgccat
    12661 cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc gagagcgatt
    12721 gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga tgtgaaaacc
    12781 gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac gatgcttgtc
    12841 tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat ggtcaaaggc
    12901 accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct ggagctggct
    12961 tttagcggcg tactgcgcca tgtcacactt aacggctcgg aagaaagcat tattactgac
    13021 gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc aagatgaacg
    13081 tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc tcggcaatgg
    13141 tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg tcgctgattg
    13201 gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg ctggggcttc
    13261 cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg ctgtttctta
    13321 accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg tccttttttg
    13381 gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag accatcgtcc
    13441 tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc attggcattc
    13501 tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt ttcgacgaga
    13561 atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc ttcaccctgc
    13621 tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg atctgtctgg
    13681 tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc ctgctcataa
    13741 ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt tactttctgg
    13801 acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt ttagcatttg
    13861 tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa gcgctggagg
    13921 atcgctcatg atggcattat tactggagcc actgcagttc acctttatga gtcacgcact
    13981 gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc tggtcctgaa
    14041 aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca tcgtgcttac
    14101 ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat tctgcgcggt
    14161 ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta tggggattgt
    14221 tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac cagacgtaca
    14281 tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata taatccagac
    14341 ggtgattatt gccgggctgg ttacacttgt tattagcgta aaatggcggg attttttgct
    14401 gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat ggctgcatta
    14461 tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag taggtatcat
    14521 tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga cacaacgctt
    14581 tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga ctggtgtctg
    14641 gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat tcgcggtcgt
    14701 gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa acgcccatac
    14761 acaggatctg ttatcaccca attagcacaa tatccgagga cagcatcgtc cgccagcgtc
    14821 cgccagccta cagaagttcg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta
    14881 tcatgcaaaa aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg
    14941 aaagtttacc agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt
    15001 ccacgataaa attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct
    15061 cccgctgacg gcgataaatc gtaagcgccc cgagaagtac gtagcgtaag gattatttta
    15121 cagacgagaa gttccagggc agcagttcat gcacttggtt cgacggccag tcattgagct
    15181 tctcgatcac ttcgcgcaac cagtcctccg gctccacttc gttctgtttg caggtgacca
    15241 gcagactgta gatgatcgcc gcactttctc ctcccttgtc tgagccgaaa aagagataat
    15301 tttttcttcc aaccgccacc gatcgtaacg cgttttcacc gatgttgttg tctatttcca
    15361 cccagccgtc acgacagaac tcgttcagcg cattccagtg attcaggata tagtcgaacg
    15421 ccttcgccat ctccgcatgt ttcgacagcg ttttcctctg caactgtatc cagtcgtaca
    15481 atgactgcat caactggacg cttctggctt ttctgactgc aagccgttcc tctgccggac
    15541 tgccacgtat ctccgcttct atgtcgtata actctgctat ccgtctgagc gcttcctgag
    15601 tcatttctgt cggacggcgc acatcctcgt catggatttt tcggcgggcg tgggccaggc
    15661 acccggcttc cttcacccgg cccgtttcgt acagtacgtt ataacctgca taggcatcag
    15721 cctgcagtac gccctgatac tttgccaggt ggagctgcgg atgttctcct ttgcgatctg
    15781 ccgaatacgc gaaccagacg gctgccggca gggatgaacc cgcattacga tcatccctga
    15841 cgtataccca cagacgaccc gttttcgtct ttccgttccc cggggcggtt cattcagcac
    15901 agatttataa acctgctgaa actcggtttc gtccatattc gcaaacgaga tggatttcgc
    15961 cctgcgccca cggctaccgt ccggataaat atgctcggtg taaaatccgg cctgaatggt
    16021 tacccactcg cggaaagcct caaacgactt tagcaatgct gtatctcggg ttctgcgtgt
    16081 cgcaactgta ttcagatatt gctctgcggc ttcgctcaga gctggcgtat gttcccggcc
    16141 tactgattcg cacaggtact caacgaagcc tgataccagt tctcgttcgc gaggcgtaat
    16201 cgccccaccg atcggagtcc agtaatcgaa tcccagttgc aggagtttga aaaaacgctt
    16261 gtggaatgcg tagttacgca cacgcttaaa gtctgcgtgt atccactcac ctgttttgat
    16321 ttgatgcaga aaatcgcaac tctccggcgt cgccgggaga agtaatccgg aagaggtttg
    16381 tttgatcagt tgtatatgcg ccatcggctt tctccggtgg cacggtgtta cacagcagga
    16441 gttcaatcct gctcaagatt gtagatgagt ttattcttca gcaaaagcag aaaaccagcc
    16501 ttaaaaccaa tctctttcaa aacccgtaat gatgtgacaa attcgtcctc acgcaaaata
    16561 aaaccgtccg tcagaagtcc attacaaaaa taaaataaca cagcaccgct cttcctttgt
    16621 tgagattgca aacatctaat gcggcaatgg ctgacaatcg ctccattctc aacgcgcaca
    16681 gcatagaggc cattttcact aaaaatttca cgcaattctt cgattttcat cttcagaatc
    16741 cttccagata aatagctctc ccctgttcgg ggtccatccc tcttctccct gcgcgctact
    16801 taagtgagtc gattctatct gcgaaggtgc gcgaatcaaa ttcaccggaa ataaacaaca
    16861 aaaaacccgc cgaaacgctg agagatcaca gcgatgggcg ggttaagtgc gggtgcgttg
    16921 aggatgcctg acacatcaga ggtgggcggg gatgggatca gctccccgcc cggtcactct
    16981 tacttcctgg attcgtagtc tacgaagaca gcgacctccg tctggccggt tcggattcgt
    17041 acctcacaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
    17101 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc
    17161 atgtaagagg ctaacctaca tgtgcaaagc atgaaattgg cctcagatta atgttaagcg
    17221 tcttgccgga cgcgtaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc
    17281 ctgaagcaga tagcctcaag cacccgcagt cattctactt acctcgccaa tatgaaatca
    17341 atcagaaagg tgccccataa aatcactcct tctcttcttt accgtagtgg agttgaccaa
    17401 ttttgataag agggcgtccc tgagatttgc ggtgtagatt ggtatcgcgc agagaataca
    17461 cacagccaca atattcctgc tgatagaatt tttcgcgctt gctgatttca atcatacggg
    17521 acgagcctcc ctgcttgcgc cagttataat cccagtacac catgcccgga taatgcgcga
    17581 cggctcgccg cccacagtcg ttaacctgct gcatattttt ccagcgtgaa atgcccagtg
    17641 aactgctgat cacactgaaa ccattttcag cggcgtacag cgctgtccgc tcaaaacgca
    17701 tgtcaaaaca catggtgcaa cggatccctc gttcgggctc ccattccatt cctttggctc
    17761 gttcaaacca gttgtctgtg tcgtaatcag catcaataaa cggcacgccg tgttgttcag
    17821 caaagcgaat attctcatcc ttacgaatta aatactcttt ctgaggatga atgttcgggt
    17881 tgtagaaaaa gatggtgtag tcgattcccg aggcctgaag cgcctccatc acctcaccgg
    17941 aacatggagc acagcaagag tgcagtagta gtttgtttgc cccgtttgg
//
LOCUS       DAJVTY010000079        17835 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVTY010000079 DAJVTY010000000
VERSION     DAJVTY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17835
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.079"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            597..962
                     /locus_tag="OQQ66_002352"
     CDS             597..962
                     /locus_tag="OQQ66_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1905259.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1002..1697
                     /locus_tag="OQQ66_002353"
     CDS             1002..1697
                     /locus_tag="OQQ66_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS1905260.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1764..3182
                     /gene="dcm"
                     /locus_tag="OQQ66_002354"
     CDS             1764..3182
                     /gene="dcm"
                     /locus_tag="OQQ66_002354"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1905261.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3163..3633
                     /gene="vsr"
                     /locus_tag="OQQ66_002355"
     CDS             3163..3633
                     /gene="vsr"
                     /locus_tag="OQQ66_002355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1905262.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3622..4542)
                     /gene="yedA"
                     /locus_tag="OQQ66_002356"
     CDS             complement(3622..4542)
                     /gene="yedA"
                     /locus_tag="OQQ66_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1905263.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4715..5632
                     /locus_tag="OQQ66_002357"
     CDS             4715..5632
                     /locus_tag="OQQ66_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1905264.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDSLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5711..5893
                     /locus_tag="OQQ66_002358"
     CDS             5711..5893
                     /locus_tag="OQQ66_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1905265.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6064..7758
                     /gene="yedQ"
                     /locus_tag="OQQ66_002359"
                     /pseudo
     CDS             6064..7758
                     /gene="yedQ"
                     /locus_tag="OQQ66_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7755..8570)
                     /locus_tag="OQQ66_002360"
     CDS             complement(7755..8570)
                     /locus_tag="OQQ66_002360"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCS1905266.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8868..9084)
                     /gene="yodD"
                     /locus_tag="OQQ66_002361"
                     /pseudo
     CDS             complement(8868..9084)
                     /gene="yodD"
                     /locus_tag="OQQ66_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9247..9435
                     /gene="dsrB"
                     /locus_tag="OQQ66_002362"
     CDS             9247..9435
                     /gene="dsrB"
                     /locus_tag="OQQ66_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1905267.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9479..10102)
                     /gene="rcsA"
                     /locus_tag="OQQ66_002363"
     CDS             complement(9479..10102)
                     /gene="rcsA"
                     /locus_tag="OQQ66_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1905268.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKHKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10387..11172)
                     /gene="fliR"
                     /locus_tag="OQQ66_002364"
     CDS             complement(10387..11172)
                     /gene="fliR"
                     /locus_tag="OQQ66_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1905269.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11181..11450)
                     /gene="fliQ"
                     /locus_tag="OQQ66_002365"
     CDS             complement(11181..11450)
                     /gene="fliQ"
                     /locus_tag="OQQ66_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1905270.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11460..12196)
                     /gene="fliP"
                     /locus_tag="OQQ66_002366"
                     /pseudo
     CDS             complement(11460..12196)
                     /gene="fliP"
                     /locus_tag="OQQ66_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(12196..12561)
                     /gene="fliO"
                     /locus_tag="OQQ66_002367"
     CDS             complement(12196..12561)
                     /gene="fliO"
                     /locus_tag="OQQ66_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1905271.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12564..12977)
                     /gene="fliN"
                     /locus_tag="OQQ66_002368"
     CDS             complement(12564..12977)
                     /gene="fliN"
                     /locus_tag="OQQ66_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1905272.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(12974..13978)
                     /gene="fliM"
                     /locus_tag="OQQ66_002369"
     CDS             complement(12974..13978)
                     /gene="fliM"
                     /locus_tag="OQQ66_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1905273.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13983..14447)
                     /gene="fliL"
                     /locus_tag="OQQ66_002370"
     CDS             complement(13983..14447)
                     /gene="fliL"
                     /locus_tag="OQQ66_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1905274.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14552..15679)
                     /gene="fliK"
                     /locus_tag="OQQ66_002371"
     CDS             complement(14552..15679)
                     /gene="fliK"
                     /locus_tag="OQQ66_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1905275.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15676..16119)
                     /gene="fliJ"
                     /locus_tag="OQQ66_002372"
     CDS             complement(15676..16119)
                     /gene="fliJ"
                     /locus_tag="OQQ66_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS1905276.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16138..17506)
                     /gene="fliI"
                     /locus_tag="OQQ66_002373"
                     /pseudo
     CDS             complement(16138..17506)
                     /gene="fliI"
                     /locus_tag="OQQ66_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(17506..>17835)
                     /gene="fliH"
                     /locus_tag="OQQ66_002374"
     CDS             complement(17506..>17835)
                     /gene="fliH"
                     /locus_tag="OQQ66_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1905277.1"
                     /translation="LMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQL
                     RVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELCR
                     LAAPGVV"
BASE COUNT         4371 a         4458 c         4702 g         4304 t
ORIGIN      
        1 cgccgggacc agcattgcca gaacttttct tttcattatg tattcccttg tgattataat
       61 cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa gcacatttgg atactattct
      121 atgaagttca tttcatttta agtattacat gcaacaaatg tatttaaaag gtttcaaaat
      181 gtttctaatc tatttaatat aattcagaga tatgaattat aaatattcat gaaaattata
      241 aatgtatatc aatatgttat gagttgagcg gaaacatagg gggtatcaat gattgttgcg
      301 ccaaacggct ggtgtgatca ggcgcacatt aatgaaggca taaaaaaaag ccggcatcat
      361 gccggcccaa aaaatataaa caagacgata aaaggctaat aacggaagca tcatgacaca
      421 gaataataaa aaggtataat tgtaatcagt gctaatatat tgattaaatg aattaataaa
      481 gccgatatca aagataaata aaatcgctca aaacgatttt tcctgtattc actgccgttg
      541 cgcaaaattt atctatttgt tcaaaaaatg attgagtctt gactggctca tccaatgtgg
      601 aaaaatgtga cttttatcac ataatagtac taagtctgaa ttttccgggt tatctcaaaa
      661 tggaatacgg ttcgacaaag atggaagaga gactctctcg cagccctggc ggcaaactgg
      721 ctttgtgggc tttctataca tggtgtggct atttcgtctg ggcaatggcg cgttacatct
      781 gggtgatgag ccggatcccc gatgcccccg tgtcgggatt tgaaagcgat ttaggctcta
      841 ccgcgggaaa gtggttaggg gcactggtcg gatttttatt tatggctctg gtcggggcat
      901 tgttaggcag cattgcctgg tacacccgac ctcgtcccgc acgtagcaga cgctatgaat
      961 aaaaactgct aaggtggcga ctctttggca acggagcgcg tatggactta caacactggc
     1021 aggcacagtt cgaaaactgg ttgaaaaacc atcatcagca ccaggacgcg gcccatgacg
     1081 tgtgtcattt tcgccgcgtc tgggctacgg cacagaagct ggcggcagat gacgatgtcg
     1141 atatgctggt gattttaacc gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc
     1201 atccgcaaag gcagcgttct tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg
     1261 aagagttcga gcaatttccg gcggagaaaa tcgaggccgt ttgtcatgtc attgccgctc
     1321 acagtttcag cgcgcaaatc gcccccttaa caacggaagc taaaatcgtc caggatgcgg
     1381 atcggctgga agccctgggg gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat
     1441 tgggcgtggc gctgtttgat ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata
     1501 aacgctacgc gctggatcat ttccagacta agttgctgaa gctaccgcaa accatgcaaa
     1561 ccgcaagagg caagcagctg gcgcagcaca acgcgcattt tttagtcgag tttatggcga
     1621 agctcagtgc cgaactggcg ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt
     1681 tttcacccgc gggataagcg cgtggcccta aatggctgta attatgttaa cctgtcggcc
     1741 atctcagatg gccggttaaa tctatgcagg aaaatatatc agtaaccgat tcatacagca
     1801 ccgggaatgc cgcacaggca atgctggaga aactgctgca aatttatgat gttaaaacgc
     1861 tggtggcgca gcttaatggt gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg
     1921 cgctggcgaa tgactcggca tggcaccgtt taagtgagaa agagttcgcc catctgcaaa
     1981 cgttgttacc caaaccaccg gcacatcatc cgcattatgc gtttcgcttt atcgatctat
     2041 ttgccggaat tggcggcatc cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta
     2101 ccagcgaatg gaacaaacat gcggtacgca cttataaagc caaccattat tgcgatccgg
     2161 cgacgcatca ttttaatgaa gatatccgcg atatcaccct cagccataaa gaaggcgtga
     2221 gtgatgaggc ggcggcggaa catattcgtc aacacattcc tgaacacgat gttttactgg
     2281 ccggtttccc ttgtcagcca ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc
     2341 gggcgcacgg ttttgcctgc gatacccagg gcacgctgtt ttttgatgtg gtacgcatta
     2401 tcgacgcgcg tcgtccggcg atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg
     2461 accagggtaa aacgttccgc atcatcatgc agacgctgga cgaactgggc tatgacgtgg
     2521 ctgatgcaga agataacggg ccggacgatc cgaaaatcat cgatggtaaa cattttctgc
     2581 cgcagcaccg tgaacgcatc gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg
     2641 attttactct gcgtgatatc agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc
     2701 tgttggaccc gatggtcgag gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct
     2761 atcgatatgc gaaaaaacat caggcgcgcg gtaacggctt cggttatgga atggtttatc
     2821 cgaacaatcc gcaaagcgtc acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg
     2881 aaattttaat cgatcgcggc tgggatatgg ccaaaggtga gaaagacttt gacgatccgc
     2941 tgaatcagca acatcgtcca cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt
     3001 ttgaagcgcc gggagaagcg aaattccgca ttccggtttc ggacactcag gcctatcgcc
     3061 agttcggtaa ctcggtggtc gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa
     3121 aaatcaaaca ggcggtggcg ttgcgtcagc aagaggcaca acatggccga cgttcacgat
     3181 aaggccactc gcagcaaaaa tatgcgcgcg attgccacgc gtgatacggc gattgagaag
     3241 cgcctcgcca gtctgttaac cgggcagggc ctggcgtttc gcgttcagga cgccagtctg
     3301 cccggaagcc cggattttgt cgttgatgaa tatcgctgcg tgatatttac ccatggctgc
     3361 ttctggcatc atcatcactg ctatctgttt aaagtgcctg cgactcgaac cgagttctgg
     3421 ctggagaaga taggtaaaaa tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa
     3481 ctcggctggc gcgtactgat tgtctgggag tgtgcattac gtgggcgcga gaagctgacg
     3541 gatgaagcgc ttaccgagcg tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag
     3601 atcgacacgc aggggattca tttactcgct tgatgcgtcc tgaataactg gcgcaactac
     3661 gggttttgcc gggaagagat attttcccaa cgtgaccagt accaccgcga agacaattac
     3721 gccgagcgcc agccattcaa tcttcgacag cgtctctccg cccagtcccg tacccagcaa
     3781 gaccgcgacc accgggttaa cgtaggcgta gctggtggcg agagccggac tgacattacg
     3841 gattaaatac atataagcgt tgatggcgat aatcgaacca aacagcgcca gatagccgac
     3901 cgcaaggaag cctgaaaggg aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc
     3961 gatcattaac accacgcctg ccgccagcat ctcaatcgca cccgccatca tccctacagg
     4021 taaggtaatg cgcgagccat aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat
     4081 cagaatcgcg ccccacggat tgccgcttaa atttccaccg ctattgagca tgatgattcc
     4141 ggcaagccca atggcgatac ccacccattc caatttgcgc gttttaatgc caaacaggcg
     4201 gctgaagcac agggtaaaga ggggcacggt tgcaaccaca acggcggcga tgccggaagg
     4261 aacattttga tgctcggcaa ccgtcaccat gccattaccg acagccagca ataacaggcc
     4321 aatcagcgcg gcattgagca gcggacgtag cggggggagc ttgtgtccgc gcagtagcaa
     4381 aaatgccatc aataaaatac cggctgccag gaatcgaacg cctgccatca ttaacggagg
     4441 ccagctttcc acgccaatcc gaatgacaaa ataggttgag ccccaaatga tatacaacgc
     4501 aaacagcgcg ccaaaaagcg gtaacaactg gcggaaacgc ataatccctc acggtggaaa
     4561 taaaaaggtg gttcatagta aacgtgaaaa tcattctgct ggcgagagat ataattgcat
     4621 ttgattgtta aaaaaatgtt gacctgtgaa gcaagtatca gagctgccgt ttttgcttca
     4681 tacttacacc cttcaacaat aaaaatgaga gggaatgctt ttggccggga gtagtttact
     4741 gacgttgctc gatgatatcg ccacactact ggacgatatc tccgtgatgg gcaaactggc
     4801 ggcgaagaaa accgccggtg tattagggga tgacttatcg ctcaatgcgc aacaagtttc
     4861 aggcgtgcgg gccaaccggg aacttcccgt ggtctggggc gtggcgaaag gatcgctgat
     4921 taataaagtg attctggtgc cgctggcgct gatcatcagt gcgtttatcc cgtgggcgat
     4981 tacgcctctg ttgatgattg gtggcgcgtt tctctgcttt gaaggagtag agaaagtgct
     5041 gcatatgctg gaggcgcgta aacataaaga agatccggcg cagagccagc agcgtctgga
     5101 gaagctggcg gcgcaggatt cgctgaagtt tgaaaaggac aaaataaaag gggcgattcg
     5161 taccgatttt atattgtctg ccgaaatcgt cgccatcacg ctggggattg tggccgaagc
     5221 gccgttgctt aatcaggtgc tggtgctttc aggcatcgcg ctggtagtga ccgtgggcgt
     5281 ctacggtctg gtaggggtta tcgttaagat tgatgacctg gggtattggc tggcggaaaa
     5341 atccagcgcg ctgatgcagg cattaggtaa agggttattg atcattgcgc cctggctgat
     5401 gaaagcgtta tcgattgtcg gcacgctggc gatgttcctc gtcggcggcg ggattgtggt
     5461 acatggtatt gcgccgctgc atcacgccat tgaacatttc gctgggcagc aaagtgcagt
     5521 ggtggcgatg atattaccga ctgttttaaa tctgattctt ggatttatca tcggcggcat
     5581 cgtggtgctg ggagtgaaag ccgtagcgaa aatacgcggt caggcacatt aatactcgca
     5641 caggctatta tgcaaaattc gccatcttca actaaggtga acaagtttca ctcgaaaaaa
     5701 ggaggcgagt atgagcttta tggttagtga ggaagttacg gttaaagagg gcggcccgcg
     5761 gatgattgtc accggatact ccagcggtat ggttgagtgt cgttggtatg acggttacgg
     5821 ggtcaagcgg gaagcttttc atgaaaccga gcttgttccg ggggagggga gtcgttctgc
     5881 ggaagaagtt tgaaagggta tcattaacac caggccggat cacttccggc ctggtgtgcc
     5941 aatgcctgca atgaatgata gtcgcgtctt atcatgccac cattataaaa ttatcgctgt
     6001 tttgcggtac gctatcgcca ggttatgcca gaatcataaa aaagcaggtt gggagtcgtc
     6061 agggtgcagc acgagacaaa aatggaaaac cagagctggt tgaaaaaaat cgcacgccgc
     6121 ctggggcctg gtcatgtcgt taatctctgc tttatcgtgg tattgctttt ttccaccttg
     6181 ctgacctggc gtgaagtggt agtgctggaa gatgcctata tctccagtca gcgtaatcat
     6241 ctggaaaacg tagccaacgc gctcgataag catttgcagt ataacgtcga caaactgatc
     6301 tttttgcgta atggcatgcg cgaagctctc gtagcgccac tggatttcac ttcactgcgt
     6361 aatgctgtaa ccgagttcga acagcatcgc gacgagcacg cctggcaaat tgaactcaac
     6421 cgacgacgca ccctgtcagt caatggcgta tcggatgcat tagtcagcga ggggaatctc
     6481 ctgtctcgcg aaaatgaaag cctcgacaat gaaattaccg ctgcactgga agttggttac
     6541 ttgctgcgac tggcgcacaa cacctcgtcg atggttgaac aggcgatgta tgtctcgcgt
     6601 gccggatttt acgtttcgac gcagccgacc ttgtttacgc gcaatgtacc aacgcgttat
     6661 tatggctatg tcacccaacc ctggtttatc ggccattcgc aatgagaaaa tcgtcaccgc
     6721 gcggtacgct ggtttacttc gcaaccggaa cacgccagca atactgaacc gcaggttacc
     6781 gtcagtgttc cggtagacag taataactac tggtatggcg tgctggggat gagtattccc
     6841 gtgcgtacca tgcagcaatt tttaagaaac gccatcgata aaaacctcga tggtgagtat
     6901 cagctctatg acagtaagct gagatttttg acctcttcca atcctgacca tccaacaggg
     6961 aatatttttg atcctcgtga actggccttg ctggcgcagg cgatggaaca tgacacgcgg
     7021 ggcggcattc gtatggacag tcgctatgtt agctgggaac gtctggacca tttcgacggt
     7081 gtgctggtgc gcgtccatac gctaagcgaa ggcgtgcgcg gcgatttcgg cagtatcagc
     7141 attgcattaa ccctgctgtg ggcgctcttt accaccatgt tactcatctc ctggtatgtg
     7201 attcgccgga tggttagcaa catgtatgtt ctgcaaagct cgttgcagtg gcaggcgtgg
     7261 cacgacacct taacccgttt atataatcgt ggcgcactgt ttgaaaaagc tcgtccgctc
     7321 gcgaaattgt gtcagacgca ccaacatcct ttttctgtca ttcaggtcga ccttgaccat
     7381 tttaaagcga ttaatgaccg ctttagtcat caggcgggcg accgtgttct ttctcatgct
     7441 gccggattaa ttagcagttc cttgcgtgcg caggacgttg ccgggcgggt cggtggtgag
     7501 gagttttgtg tgattctgcc aggcgcgagt ctgacggagg ctgcggaagt cgcagaacgt
     7561 attcgcctga agttaaatga aaaagagatg ttgattgcta agagtacgac gatacgcatc
     7621 agtgcctcgt tgggggtaag tagcagcgag gaagccggtg attatgattt tgaacaactc
     7681 cagtcactgg ctgaccgtcg gctttatctc gctaaacagg ccgggcgtaa tcgggtatgc
     7741 gcgagcgata acgcttaacg agcggagaaa aaatggtcca gcccttcacg ccatccttcc
     7801 ggtccttcac gctgcgttcg ccagacgcgg gtcggatcct catcatgcag atgcacccct
     7861 tcacggttta gccctttcac aatcaccgcg taatccatta cctccagtaa gggcgcatcg
     7921 tttggcccat cgcccaggcc aagtgtggtt gggcgtttgc ctgataattg ttgataggtc
     7981 gcgataatcc agttggcggc ctgatctttt ccggcagagg catccaggac gtgccagaag
     8041 cgcgcaccct gcataaactg taagcccagt tcgttcagac gagcggtaaa ttgtgccata
     8101 cgctcgtcac tgtcgcgcca gattagcgtt accgacgcct catgcagctg cgtcagcgcc
     8161 gcctggctac ggcttaatcc cgtccattcg gcgatggttg catcgtcgac atcatcgaaa
     8221 gtcgtgaatt taaaatgttc tttctcgcgt agcgtattta aaacctggct gatttcgcca
     8281 tggctaatac ctgagatgat gcgtggaaaa ccgtctatat cctgccattg ttcagcaagc
     8341 tggatcactg cgccattttc ggcaatcagt ggtacccctt gtaaccccaa cgttttttgc
     8401 aagtacagca tttccgctga agttttactg ctacagagaa tgacgggaac attcgcttcg
     8461 cgtaaacggc tgagccaggg ggcagccggt tgccagtcat aactatgact gtccagcagg
     8521 gtgccatcaa gatcgctaaa aaccagtagt ggttgttgaa ttgaaaacat ggtcgcgcag
     8581 ttctcccctt accaggatga ttctcaaaac agcacaaata atcaaagatg attttttcgg
     8641 gagcgatccc gttgacgcca gagccaccaa ggggtgacgc acgtcacatt tctattcata
     8701 agtagcgtta atcattcata tcgcgaatat tttcttgtca gcgaaaaaaa ttgcggataa
     8761 ggtgatgaac acatcagatt tcctggtgta acgaattttt taagtgcttc ttgcttaagc
     8821 aagtttcatc ccgaccccct cagggtcggg atttttttat tgtgcattca acgattcact
     8881 tcagagacgc tgaagtcatg aatatccagt tcgaaggcat ccgccaattc acgccatgta
     8941 tgatattcac gtccatcgac gctaacgtgt tcagaatcgt tctggctgcg atgaacttat
     9001 ttcattgatc gccgccagca gggcatcgac atcgacgctg acctcacgtt ttgcggtatc
     9061 gctgtactct tttgcggttt tcatcatctt tcctcgcaac cgttactctg ttgttgccgc
     9121 cagtcgttaa gtccgcctgg catctcttaa gtatagaccg tcgagaaaat cagcactcgc
     9181 gcgcgcctgg cgctgccaga aaaacgaaat tgttctacac tggcacaaag ccacaggagg
     9241 aaaacgatga aggtgaatga tcgggtaaca gtcaaaacgg atggcggtcc gcgtcgtcct
     9301 ggcgtggtac tagcagttga ggagtttagt gaaggcacaa tgtacctggt ttcgctggaa
     9361 gactacccgc tcggcatctg gttctttaat gaagcagggc atcaggacgg tatctttgtg
     9421 gagaaagcag agtaatctta ttcagcctga ctggtgggaa accaccagtc agaatgtgtt
     9481 agcgcatgtt gacaaaaata ccattagtca cattatccgt cagtcggacg acatggtaga
     9541 taacctgttt attatgcgtt ttgatcttat gtttaatatt acctttatgc gatgaaacgg
     9601 tcttggcttt gatattcatt tggtcagaga tttgaatggt tccctgacct gccatccaca
     9661 ttcgcaacat actcgattcg gttcggctca atgataacgt cggcatattt aaaaaagagg
     9721 ttatcgttgt ctcttttttc agaatatcgc caaggatatc gtcgagagat tccggtttaa
     9781 tcgatttaga acttatcaat aaattttttc tgaccaatag atattcatca aaatgaacat
     9841 tggcaattgc cataaaaacg ataaataacg tattgggatg ttgattaatg atgtgcttga
     9901 tgtgctgact gttagaagca tcatggatga aacagtcctc attaataaac accactgaag
     9961 ggcgctgtga atcacaagct atggcaaggt catcaacggt ttcaatgtcg ttgatttctc
    10021 tttttttaac ccctctactc aacagatacc cggttaaacc tagtcgggtg taactacata
    10081 aatccataat aatcgttgac atggcatacc ctcactcaat gcgtaacgat aattcccctt
    10141 acctgaatat ttcatcatga ctaaacggaa caacatgggt cacctaatgc gccactctcg
    10201 cgatttttca ggcggactta ctatcccgta aagtgttgta taatttgcct ggaattgtct
    10261 taaagtaaat gttgcgatat gtgagtgagc ttaaaacaaa tatttcgccg caggagtatc
    10321 ctggaagatg ttcgtaggta ttatttttcg gataatcctt aggataacat gataaacgtt
    10381 acggaattat attaatggca attcactaat aatatcagcc agcaaattaa acatctcact
    10441 gaataaatgt tcgcaaaaag gtgcaattaa cggcattaat gccgccatta aagagatacc
    10501 gacagtcaga gttaatggaa atccaataac aaaaatggat aattgcgggg ccatacgatt
    10561 cagtaaacct aatgccagat tcagggtcag cagcagagta atgagcggta acgccagcat
    10621 cagcccgtta aggaaaatca aactccccgc tttggtgagt gccagaaacg cattgctgtt
    10681 caacggttcg ccaccaattg gcagggtgtg aaaggtatcg accagcagtg aaatcaacca
    10741 taaatgaccg ttaaatgtca ggaacagcag taacgccagc atatccatga tacgcgctaa
    10801 aacgggcata ttgagatggc tggccggatc gacaaacgtc gcaaatgaca gccccatttg
    10861 cagaccgata atttcgccag cggttcgcac agcggcaaag gcaaattgca tggtaaaacc
    10921 aagcgcaatg ccgatcagga tctgctgcac ggccagccac agagcaaaga acgaaaaaac
    10981 aggaacatcg ttggcaggta atgatggggc aatgacgaac gtgatcatca ttgccagccc
    11041 cagttttacc cgtttcggca cgctgcgttc gctcagaatt ggtgcggtag aaatgagcgc
    11101 cagcacgcgc agtaacggcc agaagtacag gcttaaccag gaaagccatt gatcgcttgt
    11161 cacctgcatc attgtacggc ctacccgatg atatacggca ggttagtgaa caaggtgcgg
    11221 acgtaatcca gcaacagatt gagcatccac ggtccggcaa taataatggc aataaatacg
    11281 gcgatgattt ttggaataaa cgacagcgtc atttcgttga tctgcgtggc ggcctgcaaa
    11341 atactgatga taaggcccgt gaccagcgcc accagcaaca gcggggcagc cagtgccagc
    11401 gcgactttca tcgcttcagt ccccatcatc atgaccgatt caggtgtcat tttgcctctc
    11461 tagctgtaaa agctctgcgc cagcgaaccg accagcaatt gccagccatc caccagtaca
    11521 aacagcatca gtttaaaggg cagagcaatg gtggcggggg gaccatcatc atccccaatg
    11581 ccatcaacac gctggcaatc accaggtcga taatcaaaaa aggaatgaaa atcgtgaagc
    11641 ctatctgaaa tgcggttttc aactcgctgg tcacgtaggc cgggcgcaaa atgcgcatcg
    11701 gcacggcttc aggtccctgc aacgggccgg tattcgctag tctggcaaac aaccctaaat
    11761 ctgcctcacg ggtctgacgc agcataaact cacgtagcgg ctgcgcccct ttttccagcg
    11821 cctcctgcat tgatattttc tcttcgctga atggctggta cgcatcgaca taaattttgt
    11881 cgatcaccgg tgacataata aaaaaggtca aaaatagtgc cagccccagc aatacctggt
    11941 taggtggcgc ggagggcgtt cccagcgcgt tacgcaataa accaaaaaca atgatgatgc
    12001 gggtgaaact ggtcatcatc agtaaaattg ccggaataaa cgtcaacgag gtgatgaaca
    12061 ccagcgtctg caccgggagc gaccagcttt gtccaccgcc aggcagcggc tggctggtga
    12121 tacccggcag ttgcgcgaag gcgaggggcg taaccagcca gagaaggaca ggtgcgacag
    12181 acaacaaacg acgcatcagg atctcccact acgcttaagc aaatttttca tgaccgactg
    12241 aaaatcggtc tgcggtatct cttctgttgg tgctgaaggg ggaagtttat gcagcagatt
    12301 gatttgccct gcggtaacgc cgagcaccag ccgtgcatct tccacatcga ccaccacaac
    12361 ccgttcacgc gcgcccagcg aagcactggc gctaattttc agaccgttaa cgccagtgcg
    12421 tttcggggca aatcccaacc gttttacctg ccaggcagca gcgaggatca gggcaataat
    12481 ggcgatgagt gcgccgctga cctgcagcag tggcgcagca gaaaccggcg tggaagattg
    12541 cacagtagcg tggttattca tcactaacgg ctcaggcgat gcattcgctc agacggagta
    12601 atgatatcgg tgatccgcac gccatattta tcggcaacga ccaccacttc gccctgggcg
    12661 attaaataac cgttgatcag aatatccagt ggttcgcccg ccagaccgtc cagcgccacg
    12721 acggaccctt gcgtcagacg caacagctct ttgatggtca tccgcgtacg gcccagctcg
    12781 acggtcagct tgaccggaat atccattatc aggtcgatat cctgcaacgt tccgctgaca
    12841 tcaccaccgc caaattgctg gaacaccgcg tcggcagcgc ttttgctgct ggttgatttt
    12901 tgttcgctca acgcttcagc ccacagatcg tccattgcgc cgttgttgtc atcggccgga
    12961 ttattcatgt cactcatttg ggctgttcct cgttcagaga atttaaaatc gggttaatca
    13021 aatgttctat ccgtaacgca tactgaccgt tgagagtgcc gtactgactg gtcagcaccg
    13081 ggacgccgtc aacatgggcg atgatgcgat caggtttttc tatcggcagg acgtcgccgg
    13141 gtttcagttt taaaatctgc gacaggcgta gcgagatatc ggcaaagttg gcgaccagct
    13201 ccagctgtga atgctgcacc tggcgcacca ggttatcgcg ccagttctga tcttcattac
    13261 gcgagttttc cagcggcggg ttaaccaaca attcccgtag cggctcgatc atgctgaatg
    13321 gcagacagat attaaattcg ccggtcaggt tgccaatctc cacatggaac ggcgtgttaa
    13381 ccacaatgtc gttcggcgag gtggtgatat tggtaaattt cacctgcatt tccgaacgca
    13441 cgtactcaac ttccagcgga ttaatcgcct tccaggcgtc gctatagcct tcaagcgcca
    13501 gctttaacat gcggttgatg acgcgctgtt cggtatgggt aaactcgcga ccttccactt
    13561 tggtcgggaa gcgtccatcg ccgccaaaca ggttatccac ggcgataaac accagactcg
    13621 gtgaaaacac caccagccct gtgccgcgca gcggtttcag atggataagg ttcaggttgg
    13681 tcggcaccgg caggttgcgg gcaaattcat ggtacggctg aatgcggatg gccccgacgg
    13741 ttatatccgg gctacgacgc agcaggttga acagccccat acgaaaatgg cgggcaaagc
    13801 gctcattaat gatttccagc gcctgcaaac gttcgcgcac aacccgtcgt tgggtattcg
    13861 gatcgtacgg acgaatgtca ctttcgccgc taacactggc tgtcggttcg tctttgactt
    13921 cgctgtcacc attcaacagc gcatcaattt cagcttgaga aagaatacta tcgcccatgt
    13981 cgttatcgca gaataaaagc ggtatacagc acgtcggtga catcctgttt cggttgcccg
    14041 gcaacaagcg gggtggaaag cgtggtttta atctcggcaa tcaggttttt cttgccttct
    14101 tctgtcgcca gtacggcagc atcctgacgc gaaaacagta acagcaaacg gctacggact
    14161 tccggcaaat actcactcag ccgcgagcgg gtagcttcat ctttcaggcg cagggttatg
    14221 ccgatataaa gcacgcgatc cgcatcgccc aaattgaccg tgaaggtatt cagcgcgtag
    14281 aagaccgggg agggtacgac gcgttgctgc gctttgtcgt cagcggcaac ctgatgcgaa
    14341 tgccagtagc tgtaacctgc gctggcacag gccgcgaggg taatgaatac cagaatcggg
    14401 atccaaagcg atcgcttgct tttcttgctt atcgcgtaat cagtcatgtg ttgcggtctt
    14461 cctgtgtcgc tactgcttat cggctaatta tcctgcgttt tgagcgcaac aaagcgggga
    14521 aaagacgcgg attacggtgc tacctctgac gttaggcgaa aatatcaacg ccgctgttgc
    14581 ctgttacacg cccttgtaaa gagacgggaa ccggaagcgt atcgtcgtct tcccccgcca
    14641 gaggttcatg gtttactgtg cgttggcttt gctgttgttg ggaagcggcc tgctgctgac
    14701 cactaaagct ttcgccactg atgttgcttt gccctaactg aatgccactt tcggccagct
    14761 gcgtacgcag taccggaagc gctgcttcca gcgcggcgcg tacatgctga tgcggtgaaa
    14821 ccatctggat ttgcgcctgg ttatcatcca ctttgaggga gatttgcact tcacctaaat
    14881 cctgcgggtg cagacgcaac tctgcacttt gttgcccctg gcgggtgaac agcgaaatat
    14941 gctggcttaa tgattgttgc cattcgtgag aacccagcgg tgcactcaaa acaggcgcgg
    15001 cgacggtggg cagtggctgt gtctggtgtg gagtaattag cgggctggcg gcagcggtca
    15061 ccggtgacgg tgtgctgatg acttccgctt tactctgggc ttctgctacc agcggtgtta
    15121 atggctgagc tggcgtgccg ggggcatcat caggctgtgc tgttgtgagt tgcgcagaag
    15181 tcagttttgt gaagagcgtt ggtttctctg ccggtaacac ggttgacggc acatcagtca
    15241 ctttgggcgt attgtcaaaa cccggcaaca tcgcaaaaag ggcgctcagg cttgcggtga
    15301 cgtcttcatt cagatcatcc gctttttcgt cttttgtcgt attttggtca gccaccgcag
    15361 ccaacgtcat cgtctgagcg gtggttaacg gtgttgatgt ggattgttcg tcgttgatga
    15421 caggcagtgt ttcatccacg gggatcagta aatccgcttg ttgcgcgtcg gcaagaatat
    15481 cgctgatcag cggctcgcct tttgtcgtgg gcttatctgt tgccaccagc aactgggggg
    15541 ccgctttgtc ggtagttgtc tcgcctgcta atgcttcgct caacaacgcg agaaaatctt
    15601 gtgcagcatc gctggctttg ccgccaggca atgtggtggt gtcaacgtcg gcggtaatta
    15661 agggcgctaa gcgaatcatt caggtttcct catggcggcg cgctgggcga actcatccat
    15721 ctttttctga tcgaggcggt tttctgcaag cagtgccgcc gtggatagcc gttcctgcag
    15781 tgtctgccag gcctgcaaac gttgtttctt ttctcgccag ctgttcaggg caatgtcaac
    15841 tttctgcgtc cactgattaa gttgctggcg atgctgagta atggcttttt ccagcgtctg
    15901 gataaactgc tgatagttga tccagcggtt gctggtcatc ccggcactca tatcgctgtt
    15961 gaggttattg cgatattcat tctgataatc gatcagcatt ttgagctgtt cttccgcctg
    16021 ctgacacccg cgacgcattt cacccagcag gcgcgcggca tcctctacct ctttttctgc
    16081 cagatctttc agggtcgcca gcgcgccatg ttctgccatc tgccgttatc tcctgggtta
    16141 tgacactgtc gggaaaatac gctccagccc ctggagagac gcttcccagt ccgcgcgttc
    16201 aaaaatgcct tgttgcaaat agccctccag ctgcggccac agggcgatgg ctttgtcgag
    16261 catcggatcg ctgcctttgg catacgcgcc gacgctaacc agatcgcggt tacgctgaaa
    16321 actcgacaac agctgtttga aggtgcgcac tcgcgcgtaa tgttgctcac tgatcaacgc
    16381 cgtcattgcg cggctgatcg acgcttcaat atcgatagcc ggatagtgcc cggcttccgc
    16441 cagtcggcga gacagcacaa tgtgaccgtc gaggattgcc cgcgcagagt cggcaatggg
    16501 gtcctgctgg tcatcgcctt cagtgagcac ggtataaaac gcggtaatcg atccgccgcc
    16561 gctaatgcca tttccggcac gctcgaccag tgccggtaat ttggcaaaca ccgacggggg
    16621 ataaccttta gtggcgggtg gttcgccaat cgccagcgca atttcacgct gggccatcgc
    16681 gtagcgggtg agggagtcca taatcagcaa cacatgctga ccacgatcgc gaaaatcttc
    16741 ggcaatgcgc gtggcatagg cggcaccctg cattcgcagg agaggagaaa catccgccgg
    16801 agcggcaatc accacggagc gtgcacgccc ttcggcaccg aggatattct caataaaatc
    16861 ttttacttcg cgtccgcgtt caccaatcaa acccacgaca atgacatcgg cgcgggtgta
    16921 acgtgccatc atccccagca gcacactttt accaacgccg gacccggcaa acagccccat
    16981 acgctgccca cgcccaacgg taagcagggc attgattggg cgcacgccgg tgtccagcac
    17041 atgttcaatc ggtgtacgtt gcaacgggtt aaatggcggg gtaatcagcg caccggtttc
    17101 cgtcgtatcg ggggaggcca tcgagcggtt taccgctgcc gtccagaaca cgacctaata
    17161 acgccggacc gagcggtaac tgtttgccgc tttgcagccc ttctgccgaa atgtttttgg
    17221 cataaacacg cgcgccgggc aggacacctt cgacttcctc cagcggcatt aaaaacagcc
    17281 gttgaccgtt aaagccaacg acttcacttt ctacttcgtg cgtttcagtg ccgttctggc
    17341 gctcaatcac acaggttgcg ccgagcggca attgtaatcc ggtggcttcc agcaccagcc
    17401 cggtagcgcg ggttaatcgc ccgtagcgac gtaccgcagg caactgcgcc attttggctt
    17461 caaagttatc cagcgtggtt agccagcgag tcaggcgcgt ggtcattaca ccactcctgg
    17521 tgctgccaga cggcagagtt cttgccagcg agtggcgaca ctggcgtcga gatcgccttc
    17581 atcggcggag actttacagc cgccaggatg gagggtggga tcgccccgca agcgccagcc
    17641 atgcaaactt aaggtagcgc cgagcatatc atccacacgt tgcagatcat ccgggtgcac
    17701 gcgcagctgt ggtttaccgc tgaataacgg ttcttgctgc aacaactgtt ggatctgttt
    17761 gatgagtgcc gagttatcca ctgttggcgt ctgaccgatg acctgacgtg ctgcctccag
    17821 cgccatctgc atcag
//
LOCUS       DAJVTY010000080        17735 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVTY010000080 DAJVTY010000000
VERSION     DAJVTY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17735
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.080"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1773
                     /gene="fhuE"
                     /locus_tag="OQQ66_002375"
     CDS             <1..1773
                     /gene="fhuE"
                     /locus_tag="OQQ66_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCS1905278.1"
                     /translation="ESRWNLGDALSDMALFERVEVVRGATGLMTGTGNPSAAIDMVRK
                     HATSREFKGDVSAEYGSWNKERYVADLQSPLTEDGKIRARIVGGYQNNDSWLDRYNSE
                     KTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWGGLPRWNTDGSSNSYDRARSTAPD
                     WAYNDKEINKVFMTLKQRFADTWQATLNATHSEVEFDSKMMYVDAYVNKADGMLVGPY
                     SNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYELFGRQHNLMFGGSYSKQNNRYFSS
                     WANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTTHMKSLYAATRVTLADPLHLILGA
                     RYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWSTYASYTSIFQPQNDRDSSGKYLAP
                     ITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQSTGTPIPGSNGETAYKAVDGTVS
                     KGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVNPNLPRTTVKMFTSYRLPVMPELT
                     VGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLFTRYQVTKNFSLQGNVNNLFDKTY
                     DTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(1833..3266)
                     /gene="ptsG"
                     /locus_tag="OQQ66_002376"
     CDS             complement(1833..3266)
                     /gene="ptsG"
                     /locus_tag="OQQ66_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1905279.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3561..4358)
                     /locus_tag="OQQ66_002377"
     CDS             complement(3561..4358)
                     /locus_tag="OQQ66_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1905280.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4369..5373)
                     /gene="holB"
                     /locus_tag="OQQ66_002378"
     CDS             complement(4369..5373)
                     /gene="holB"
                     /locus_tag="OQQ66_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1905281.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYTVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5370..6011)
                     /locus_tag="OQQ66_002379"
     CDS             complement(5370..6011)
                     /locus_tag="OQQ66_002379"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1905282.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6001..7023)
                     /gene="yceG"
                     /locus_tag="OQQ66_002380"
     CDS             complement(6001..7023)
                     /gene="yceG"
                     /locus_tag="OQQ66_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1905283.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7026..7835)
                     /gene="pabC"
                     /locus_tag="OQQ66_002381"
     CDS             complement(7026..7835)
                     /gene="pabC"
                     /locus_tag="OQQ66_002381"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1905284.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(7955..9196)
                     /gene="fabF"
                     /locus_tag="OQQ66_002382"
     CDS             complement(7955..9196)
                     /gene="fabF"
                     /locus_tag="OQQ66_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1905285.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9284..9520)
                     /gene="acpP"
                     /locus_tag="OQQ66_002383"
     CDS             complement(9284..9520)
                     /gene="acpP"
                     /locus_tag="OQQ66_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1905286.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9731..10465)
                     /gene="fabG"
                     /locus_tag="OQQ66_002384"
     CDS             complement(9731..10465)
                     /gene="fabG"
                     /locus_tag="OQQ66_002384"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1905287.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10478..11407)
                     /gene="fabD"
                     /locus_tag="OQQ66_002385"
     CDS             complement(10478..11407)
                     /gene="fabD"
                     /locus_tag="OQQ66_002385"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1905288.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11423..12376)
                     /gene="fabH"
                     /locus_tag="OQQ66_002386"
     CDS             complement(11423..12376)
                     /gene="fabH"
                     /locus_tag="OQQ66_002386"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1905289.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12444..13514)
                     /gene="plsX"
                     /locus_tag="OQQ66_002387"
     CDS             complement(12444..13514)
                     /gene="plsX"
                     /locus_tag="OQQ66_002387"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1905290.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13595..13768)
                     /gene="rpmF"
                     /locus_tag="OQQ66_002388"
     CDS             complement(13595..13768)
                     /gene="rpmF"
                     /locus_tag="OQQ66_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1905291.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(13820..14341)
                     /gene="yceD"
                     /locus_tag="OQQ66_002389"
     CDS             complement(13820..14341)
                     /gene="yceD"
                     /locus_tag="OQQ66_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1905292.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14540..15124
                     /gene="yceF"
                     /locus_tag="OQQ66_002390"
     CDS             14540..15124
                     /gene="yceF"
                     /locus_tag="OQQ66_002390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1905293.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15236..16195)
                     /gene="rluC"
                     /locus_tag="OQQ66_002391"
     CDS             complement(15236..16195)
                     /gene="rluC"
                     /locus_tag="OQQ66_002391"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1905294.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            16481..17500
                     /locus_tag="OQQ66_002392"
     CDS             16481..17500
                     /locus_tag="OQQ66_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS1905295.1"
                     /translation="MKYTPVGVGIAKHVIQIHFINEHTDEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         4444 a         4766 c         4332 g         4193 t
ORIGIN      
        1 gaatcgcgct ggaatctggg cgatgcactt tctgatatgg cactgtttga acgcgtagaa
       61 gtagtgcgtg gcgcgacagg actcatgacc gggacgggta atccatctgc ggcaattgat
      121 atggttcgaa aacacgcgac cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt
      181 agctggaaca aagaacggta tgtggcggat ttacaaagcc cactcaccga agacggtaaa
      241 atccgcgcgc gaattgtcgg cggctaccag aataacgact catggctgga ccgctacaac
      301 agtgaaaaga ccttcttctc cggcattgtc gatgctgatt taggcgatct tacgacgctt
      361 tcagccggtt acgaatatca gcgcattgat gttaatagcc ctacctgggg cggtttaccg
      421 cgctggaata ctgatggcag cagcaacagt tacgatcgtg cacgcagtac cgcacctgac
      481 tgggcgtaca acgataaaga gatcaacaag gtctttatga ccctgaagca gcggtttgct
      541 gatacctggc aagcgacact gaatgccacc cactctgaag tcgaatttga cagcaaaatg
      601 atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc tggttgggcc atacagtaat
      661 tatggacctg gctttgatta tgtcggcggc accggttgga acagtggcaa acgtaaagtt
      721 gatgcgctag atttgttcgc tgacggtagt tatgaattgt ttggtcgtca gcacaatcta
      781 atgtttggtg gcagttacag caaacaaaac aatcgttact tcagttcatg ggccaacatc
      841 ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca aaccgactgg
      901 tcaccacaga gcctggcaca ggacgatacc acacatatga aatcgttata tgctgccact
      961 cgagtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgctatac caactggcgg
     1021 gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg
     1081 tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa
     1141 aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg
     1201 ggtctgaaat cgggctggat gaatagccgt ctgaccacca cgttagccat cttccgtatt
     1261 gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc
     1321 gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact caacggcgca
     1381 attaccgaca actggcagct gacatttggc gcaacgcgct atattgcaga ggataacgaa
     1441 ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg
     1501 ttgcctgtca tgccagagtt gacagtcggc ggtggtgtga actggcaaaa tcgcgtgtat
     1561 accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg
     1621 gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac
     1681 ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg cgcaccgcgt
     1741 aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc
     1801 tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc
     1861 tcggttttca ggttatcgga tttagtaccg aaaatcgcct gaacaccaga acccgcaacc
     1921 actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca
     1981 acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca
     2041 aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc atcttcagtc
     2101 gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg
     2161 tagtaaacaa tcgcataacc gataccgacg atcgggaaca gccacagttt gctgctgtta
     2221 ccagacagaa cgataaagtc gatcagaccg tgcgagaacg acgtaccgtc atgcatcccc
     2281 agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc
     2341 ggcgcaacga acatgaagga gaactcgatc ggctcggtga taccggtcag gaacgaggtc
     2401 agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc
     2461 cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt
     2521 ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct
     2581 gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga
     2641 ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc
     2701 tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag
     2761 gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac aaagcgttta
     2821 ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac
     2881 gctgcgatcg caccggagat aatccctccg agtacgccag tatccgccag gtgtttagag
     2941 gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc
     3001 atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg
     3061 agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct
     3121 gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc
     3181 agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc gaccttttgc
     3241 aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt
     3301 tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc
     3361 tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc tatcacgttt
     3421 caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa
     3481 acgcggttat tcaccgccca attttcggcg gtgaacttta ctcgttttag ccattaatta
     3541 cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac
     3601 agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac
     3661 acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc
     3721 ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag ggggacataa
     3781 cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg
     3841 taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt
     3901 acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcacgggcg
     3961 tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg
     4021 aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag cccggtttca
     4081 cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga
     4141 tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc
     4201 agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca
     4261 tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc
     4321 agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg
     4381 aaccggtagc acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat
     4441 gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc
     4501 ccccagtata gcctgcaggc gcgagggaga aagatggttt gccagttcgg cgaccaggcc
     4561 cggcacatca acattggtca cctgtgcagc gccatgatgg cgttttagcg catccatcag
     4621 caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgccagcag
     4681 cgaataccag tcgcccgatg gtacgctata tgccaacgcc tgacacaatg tttcacgagc
     4741 ctgccagtta tctccctgaa acaacgccag tgccgcgcta ggcgaaccgg cacttaagcg
     4801 caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggt
     4861 gtactgttcc ggcggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag
     4921 taaacgttca ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg gctcttcaag
     4981 cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac
     5041 aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc
     5101 atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt
     5161 gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg
     5221 ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa
     5281 cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc
     5341 gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac ccagtgggtc
     5401 acggtagtac ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga
     5461 atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg
     5521 aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt taagccaact
     5581 tccggggtaa catcgagata gagcgttaag tcagggcgaa aatccccgag aacagcatca
     5641 cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata cgcctgagtg
     5701 gagagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagagctgg tttgatgacc
     5761 gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg
     5821 acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc
     5881 gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc aagcgtctca
     5941 accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac
     6001 ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact tgttatggct
     6061 ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg
     6121 cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag ctatcgcacc
     6181 tggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg
     6241 agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt cggtctgcag
     6301 gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc gttcactggc
     6361 aacggcggtt tctttttcga taattgatgc catcgtcacc agctggtttt tatctttata
     6421 aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg
     6481 cgctcgcttg agtaacgcga catcggtggt attggcggta tacatccagg tgtctggcca
     6541 gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta
     6601 cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc
     6661 gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccag
     6721 caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa
     6781 gtgagaaaga tccggttcga tacgcagcaa ccattgaaaa acccgtgggc gattgatgat
     6841 cttatcggca taaagctgtt caccaagcgc cagacgtccg gtccctggct tcagggtaaa
     6901 tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc
     6961 cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata acactttttt
     7021 catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa
     7081 aaggagacat cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc
     7141 atctcatctg cctgcaacgc ctcttccaga gaggcttgca cttcgacaag ctgataagag
     7201 gattgtgcca gcaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc
     7261 agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc acagcattcc
     7321 gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga
     7381 gagcggatca atacttgctc aagccgatta agatgtttaa tacctgcaag atggggattg
     7441 cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa
     7501 tgtgcaggat aaaccgtaac ggagagaatc cgcgttgccg gtccactgtt caatgtgctg
     7561 taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt ttgctgttct
     7621 gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcacagaa aatcatcaac
     7681 cgctgacaag catcctgtag tcgctggata tgcgccgata acaaactgac tttaccgtcg
     7741 ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc
     7801 agcgattcct gcttatgacc gttaattaag aacataccgg ctccttatca gagaattgtt
     7861 agtgtggcag catgctcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc
     7921 aagcggacct tttatatggg tgggaaatga caacttagat ctttttaaag atcaaagaac
     7981 cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac
     8041 gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc caggttatcc aggttgatgg
     8101 ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagactct actgcccccg
     8161 ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag
     8221 cttcaccgaa gatggctttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag
     8281 taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat
     8341 ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat
     8401 cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt
     8461 gttcgtactc ttctagtacc agcataccgg caccatcgcc cagtacgaag ccatcacgct
     8521 ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg
     8581 ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca
     8641 tcacgtcagc atcgccatac gcgataatac gcgcagcatg accaatgttg tgcacgccgg
     8701 aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat
     8761 gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac
     8821 caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc
     8881 caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca
     8941 tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt
     9001 tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat
     9061 aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt
     9121 tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta
     9181 cacgacgctt agacacgttt gtcctccagg gagggaaaaa atgattctag tgggacaaaa
     9241 agataaaact caggcggtcg aacgaccgcc tggagatgtt cacttacgcc tggtggccgt
     9301 tgatgtaatc aatggcagcc tgaacggtgg tgattttctc agcttcttcg tccggaatct
     9361 cagtatcaaa ctcttcttcc agagccatta ccagctcaac ggtgtcaaga gaatccgcgc
     9421 ccaggtcttc aacgaaagaa gcattgttgg taacttcttc ctgcttaacg cccagctgtt
     9481 cgccgataat tttcttaacg cgttcttcga tagtgctcat actcttaaat ttcctatcaa
     9541 aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc
     9601 ccggcaggtc ttaccacgat tttacgttat tttgaggcct taccccccaa taacgcaaat
     9661 cattttgcac taattattac tcataaccac gcagactgcg cgcaacatga gcattttgtg
     9721 caaatcgcgg tcagaccatg tacatcccgc cgttcacatg caaagtttca cccgtgatgt
     9781 aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga
     9841 ggcgacccgc aggaacctgc gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg
     9901 tcatgtccgt ttcaataaag cccggagcaa caacgtttac agtaataccg cgtgacgcaa
     9961 cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg
    10021 cctgaccgcc atttcccatg gtaccaacca cagaaccgat agtgataata cgaccatgac
    10081 gctttttcat catagcgcgc attaccgctt ttgacagacg gaaaacagat gaaaggttgg
    10141 tttcgataat atcgttccac tcttcatctt tcattcgcat taacaggtta tcacgagtga
    10201 taccggcatt attgaccagg atatccactt caccaaattc tgcgcgaatt ttttccagaa
    10261 cagattcgat agatgccggg tcggtcacat tcaacatcag acctttgccg ttagcaccta
    10321 aataatcact gatcgcctga gcgccatttt cactggtcgc agtgccaata actttcgcgc
    10381 cacgggctgc gagcgtttca gcaattgcgc ggccaattcc gcggcttgca ccggttacca
    10441 gtgcgatttt tccttcaaaa ttcatgattt tcctctttta aagctcgagc gccgctgcca
    10501 tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc
    10561 cagttagcac tttgcccggg ccgacttcat agagatgttc tacgccttgc gctgccatgt
    10621 actcaacaga cttcgtccac tgaaccgggt tatacaactg acgtaccagt gcgtcacgaa
    10681 tggcatcacc attggtttcg catttcacat caacgttatt cacaacagga actgttggtg
    10741 cgttaaaggt gattttcgct aattctactg ccagtttgtc ggctgctggt ttcatcagcg
    10801 cacagtgaga cggtacgctc actggtaacg gcagcgcgcg ttttgcgccc gcagctttac
    10861 aggcagcgcc agcacgctca accgcctctt tatgaccggc aataaccacc tgtcccggag
    10921 agttaaagtt taccggagaa acgacctgac cttctgcagc ttcttcacac gctttcgcaa
    10981 tagacgcatc atccagaccg atgattgccg ccatagcgcc cgtgccttct ggtacggctt
    11041 cttgcatgaa cttgccgcgc atctcaacca gacgcaccgc atcagcgaaa tcaatcacgc
    11101 cagcgcaaac caacgcggag tattccccca ggctgtgacc ggccatcatt gccggtgctt
    11161 taccgccctg ctgctgccat acgcgataca gcgcaacaga tgcagtcaac agcgctggct
    11221 gggtttgcca ggttttattc agttcttcag ctggcccctg ctgggtcagc gcccacaggt
    11281 cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc gacaattgga tagctcgccg
    11341 ccatatcagc cagcattcca acggtttgag aaccctgtcc agggaacaca aatgcaaatt
    11401 gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc ggagccccag gtgaatccac
    11461 cgccaaaggc ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg cgtacagctt
    11521 catccagcgc gcacgggaca gaggccgcag aggtattacc gtggcgatcc aacgtcacca
    11581 cgacattgtc catagacata ccgagttttt tcgccgttgc actgataata cgcaggttag
    11641 cctgatgcgg aaccagccag tccagttgag aacggtcaag attattcgcc gccagcgtct
    11701 catcaacgat gtgcgccagt tccgttaccg caaccttgaa gacttcgttg cccgccatcg
    11761 tcagatgaat tgaattctct ggattcacgc ggtcggcgtt tggcagcgtc agcaattcac
    11821 cataactacc gtcggcatgc agatgggtgg agatgattcc cggctcttca gaggcagcca
    11881 gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt cccacgatct gttggatcgc
    11941 aggtgcgcgc cagtacatcg gaaccgacga ccagagcata cttcaccgcc ccagatttca
    12001 cgtattgatt ggctacgctt aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa
    12061 atgccgggca acctttaatg cccaacatgc tttgaatctg acaagctgcg ctcgggaaag
    12121 cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat ctggtctttc tcaatgcccg
    12181 ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg
    12241 cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat ccactcgtca gaggtgtcca
    12301 ccattttttc caaatcggcg tttgtccgca cttgttcggg cagatagctg ccagtaccaa
    12361 taatcttcgt atacatgtac gctcagtcac ttttcggtta tataccgtca cttgcaaact
    12421 gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc ttttgccacc gtccagcagc
    12481 tcaaaaccag ctgggtatac agattccagg cgagcggcaa ttcgctgagg aacttgtcgc
    12541 tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca
    12601 tgacttttta tcaccgtgcc gcgcaatcct aacagacagg cgccgttata ctggtcgggg
    12661 ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa taacagtagc
    12721 caccacgacc gttttttccc ttcaccctga gatttcagca gagaaaggaa catcctgaca
    12781 acaccttcca tcgtctttaa cgtgacattt cctgtaaagc cgtcacaaac cagcacatct
    12841 gtcttgccag ttaacaactc attggcttca agatagccga tataattgat agaagggatt
    12901 gttttaagca ccgctgaggc atcccgaata ctgtcgagac cctttacttc ttcttcacca
    12961 atattgagca acgccacgcg aggattggga atttccacca cctcttcagc cagaactgag
    13021 cccataatgg caaattgcac caacattgtg ctgtcacaat cgacgttggc ccctaaatcg
    13081 aggaccaccg ttttgccctt ttgctgatgt ggtaataccg tcaccagcgc cggacgctca
    13141 atcccctcca ggggcctgag taataatttt gccagcccca tcagtgcccc ggtattaccg
    13201 gcactgacac aggcttgcgc tcgaccttct ttcaccagct ccagggccat acgcattgaa
    13261 ctcccacgac tagcgcggat agcttgcgaa ggccgggcat cactggcgat aactgactgc
    13321 gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg
    13381 atggcgtcgg gattgccgac taaaagaaga gtgagttgcg aattagaatt cagtgcctgc
    13441 aatgctgcag gcactgtcac ggaagggcca aaatcccctc ccatgacatc taacgccagg
    13501 gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt gcctggggaa
    13561 aatcctcact aagcttcatc acgcagacgc gtgattactt agcgatgacc ttgcggccgc
    13621 ggtagtaacc gtcggcagtg atatggtgac gcaggtgttt ttcaccagaa gttttgtcta
    13681 cagacaggct ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca cgtttggaac
    13741 gggttggttt attctgttgt acggccatgg accttactcc tcaattacgg tttatccccg
    13801 atccaacggg gagcaccaat tacttacgct ttaagctggc taatacggca aatgggtttg
    13861 gcttttgcac ttcttcaggc agttcaccaa agaccatgtc cgcttcggac acttcacagt
    13921 gttcagaatc atgcaccgga actaccggca aggcgaggat gatttcatct tcaaccattg
    13981 caagcagatc gatttcaccg aattcgttaa cctcaatcgg ttcatacgct tccggcagtg
    14041 cttcagcctg ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag acctgatgag
    14101 taaacggctt cccgcaacgc tgacactcga gcgttaccgt caccttcgca tcgccgttta
    14161 acactgcgag acgttggtta tcgatagcga acgacatgga gcattccaca tcactgtcca
    14221 cactgactac ggattcggcg acgcgctcaa cctgatcagg ggtatagata ccctggtaat
    14281 caaggcgttt ttgagccgta cgaaccggat cgagagtcag gggtaatttt accttttgca
    14341 tagggcgcgc atattaactt tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt
    14401 gccttttgcc aataattcgc acacattgcg ggttactgct ttattttcgt tcagtgctga
    14461 taacgaacat caacctgcaa aacgaagcgc atagtttaaa atggctatca tcaatacgct
    14521 atatctgatg gaaaaaaata tgcctaaact tattttagcc tccacctcgc catggcgtcg
    14581 cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca gcaccagagg tcgacgaaac
    14641 cccacgcagc gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca
    14701 atctctggcg tcacgttatc cggatcattt aattattggt tcagaccagg tatgtgttct
    14761 tgatggcgaa atcaccggta aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa
    14821 agccagcggc aatatcgtca ccttctatac tggactggcg ttgtttaatt cggcgaatgg
    14881 gcatctacaa acagaagtgg agccttttga cgtccatttc cgtcacctgt gcgaggcgga
    14941 gattgataat tacgtgcgta aggagcatcc cctgcactgc gcgggtagct ttaagagtga
    15001 aggatttggc attacgctgt ttgagcgttt agagggacgt gaccctaaca cgctggttgg
    15061 tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg
    15121 atagttgttt gcgccggatg cggttacgtc gcatccggca atcggtgcag gcggcctgat
    15181 gcggcgtaaa cgccttatca ggccaaccgc ccattatcag gcttatatgc ttgtattagc
    15241 gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc atccatcggc gcttcgatac
    15301 gcatcacctc accggtcccc ggatgggtaa acttcaacgc tgcggcgtgc aggaacagac
    15361 gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa ttcacggtca ccgtagcgat
    15421 cgtcaaaggc aatcggatga cccgcatact gtgtatgcac acggatctga tgagtacgtc
    15481 cggttaccgg gctacaacgc accagggtgg caaatgcata gcgttcttcc actttaaagc
    15541 gtgtttccga cggtttgcct tcctgactga cacgcacgat acgttcgccg ctttgcagaa
    15601 tatttttcaa taacggcgct tgaacgctct tcacatgcga ctgccactga ctgcgcacca
    15661 gcgccaggta atctttttgc attccttttt cacgtaattg ctcatgcaga gaacgcaacg
    15721 ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc ccggtcgaga cgatgaacca
    15781 gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc ttcgataacg ccgaagctta
    15841 aaccactgcc gccatgtacc gccgtaccgg aaggtttatt cagcaccagg atatgatcat
    15901 cttcatataa gatgacgtcc gccagcgccg ccaccttttg cagatgtggc gaaaccgcct
    15961 cttcttcccg ctcagcaacg cgaaccggtg ggatacgcac ctcatcaccc gcttcgagtt
    16021 tatattcagg cttaatacgt tttttgttca cccgcacttc gcctttacgc aaaatacggt
    16081 aaatcatact ttttggtacg cctttcaatt gggtacgcaa aaagttgtcg atacgttgcc
    16141 ccgcttcgtc agcggtgata gcaacaattt ttacggatgg agtctctgtt ttcatggtgg
    16201 gcgattctaa ttagccaaca ggattcgcgc cactcatttt tctatgctta tatttagttt
    16261 tacaccttat tacttcacta cgtgatcact ttattgatgg ttattaaacc aatcaccagc
    16321 aagaagtgaa aaatgtaatg aacgcatccc acacaattaa acacggagca gtatgatggt
    16381 taaaagggtg aaactggcag gtgtggagcc tcgcagattt ccggcatcat agtgcccaat
    16441 acgggatgca taaagctccc tcagactgga gactccacaa atgaaatata caccggttgg
    16501 cgttggtatc gcaaaacatg tcattcagat tcacttcatc aatgagcaca cagatgaagt
    16561 ggttgataaa cagttgcgta gacaggattt tctgacgttc ttcggcaacc gtgagccatg
    16621 cctgattggt atggaggcct gtggaggttc tcagcactgg gcacgggaac tgacaaaact
    16681 tggtcataaa gtccggttgt tgcaggcccg cttcgttaag gcattcgtca tgggcaataa
    16741 gaatgatgtg atggatgccc gggctatctg gatggcggtt cagcagccgg gtaaagaaat
    16801 cgccgtaaaa acagaagaac agcagtcggt actggttctg caccgtaccc gcatgcaact
    16861 ggtgaagttc cggaccgcac aaattaatgc cctgcacggg acgttactgg agtttggtga
    16921 aaccatccac aaaggccggg cagcgatgga gcgggagttc cccgaagcac tggaacggat
    16981 gaaagagaga ctgccaccgt atctcattat ggttctggaa aaccagtaca accgactgaa
    17041 tgagctggac tcactgatag aggatattga aaaacagctt accagcgtgg cgaggcagaa
    17101 tgaaacctgt aagcggttgc tggatattcc tggcgttgga ccacttattg cgacggcagc
    17161 ggtggccacc atgggggaag catcagcgtt taaatcgggg cgagagttcg ccgcatatgt
    17221 tggtctggtt ccaaaacaaa ctggctccgg agggaaagta cgtctgctgg ggataagcaa
    17281 acgtggtgac acttatctca ggacattatt tatccacggt gcaagagcgg tggcattagt
    17341 agctaaagag cctggcccgt ggataaccga actgaaaaaa cgtcgtccag ccagtgtggc
    17401 aatcgtcgcc atggcaaaca agctggcacg aacagtatgg gcgataaccg cccatgaccg
    17461 taagtatgac aggaaccacg tcagtatcag accatattaa tcgctgatac cattaaacaa
    17521 tgaactctta acaaaagggt gaatgctgaa aggttgctat ggcggccaga gtgatgacaa
    17581 agacaggtaa gaccgtgact cactaaacct gaacagtatt ttgggcttga agtccgccgt
    17641 gaaaataagg ggtgagtcgg cgaattacat aggggctcgc agcgttacgg ctgcaataaa
    17701 gccggatata aagctgcaac ctacccgtca tgtca
//
LOCUS       DAJVTY010000081        17348 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVTY010000081 DAJVTY010000000
VERSION     DAJVTY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.081"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..589
                     /locus_tag="OQQ66_002393"
     CDS             <1..589
                     /locus_tag="OQQ66_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1905296.1"
                     /translation="TKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTA
                     ELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELP
                     TIDLYIDQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFER
                     VPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(660..1109)
                     /gene="rplI"
                     /locus_tag="OQQ66_002394"
     CDS             complement(660..1109)
                     /gene="rplI"
                     /locus_tag="OQQ66_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1905297.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1151..1378)
                     /gene="rpsR"
                     /locus_tag="OQQ66_002395"
     CDS             complement(1151..1378)
                     /gene="rpsR"
                     /locus_tag="OQQ66_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1905298.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1383..1697)
                     /gene="priB"
                     /locus_tag="OQQ66_002396"
     CDS             complement(1383..1697)
                     /gene="priB"
                     /locus_tag="OQQ66_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1905299.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1704..2099)
                     /gene="rpsF"
                     /locus_tag="OQQ66_002397"
     CDS             complement(1704..2099)
                     /gene="rpsF"
                     /locus_tag="OQQ66_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1905300.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2426..2701
                     /locus_tag="OQQ66_002398"
     CDS             2426..2701
                     /locus_tag="OQQ66_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1905301.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2776..3327)
                     /locus_tag="OQQ66_002399"
     CDS             complement(2776..3327)
                     /locus_tag="OQQ66_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS1905302.1"
                     /translation="MAINGLFVRTEPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3424..4110)
                     /gene="ulaF"
                     /locus_tag="OQQ66_002400"
     CDS             complement(3424..4110)
                     /gene="ulaF"
                     /locus_tag="OQQ66_002400"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1905303.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4110..4964)
                     /gene="ulaE"
                     /locus_tag="OQQ66_002401"
     CDS             complement(4110..4964)
                     /gene="ulaE"
                     /locus_tag="OQQ66_002401"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1905304.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4974..5624)
                     /gene="ulaD"
                     /locus_tag="OQQ66_002402"
     CDS             complement(4974..5624)
                     /gene="ulaD"
                     /locus_tag="OQQ66_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1905305.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5638..6102)
                     /gene="ulaC"
                     /locus_tag="OQQ66_002403"
     CDS             complement(5638..6102)
                     /gene="ulaC"
                     /locus_tag="OQQ66_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1905306.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6112..6417)
                     /gene="ulaB"
                     /locus_tag="OQQ66_002404"
     CDS             complement(6112..6417)
                     /gene="ulaB"
                     /locus_tag="OQQ66_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1905307.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6433..7881)
                     /gene="ulaA"
                     /locus_tag="OQQ66_002405"
     CDS             complement(6433..7881)
                     /gene="ulaA"
                     /locus_tag="OQQ66_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1905308.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8185..9249
                     /gene="ulaG"
                     /locus_tag="OQQ66_002406"
     CDS             8185..9249
                     /gene="ulaG"
                     /locus_tag="OQQ66_002406"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1905309.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTPPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9357..10112
                     /gene="ulaR"
                     /locus_tag="OQQ66_002407"
     CDS             9357..10112
                     /gene="ulaR"
                     /locus_tag="OQQ66_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1905310.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10109..10858)
                     /gene="yjfP"
                     /locus_tag="OQQ66_002408"
     CDS             complement(10109..10858)
                     /gene="yjfP"
                     /locus_tag="OQQ66_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1905311.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11040..11369
                     /gene="bsmA"
                     /locus_tag="OQQ66_002409"
     CDS             11040..11369
                     /gene="bsmA"
                     /locus_tag="OQQ66_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1905312.1"
                     /translation="MVSRKRNSVIYRFACLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11518..11793
                     /gene="yjfN"
                     /locus_tag="OQQ66_002410"
     CDS             11518..11793
                     /gene="yjfN"
                     /locus_tag="OQQ66_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1905313.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11910..13535)
                     /locus_tag="OQQ66_002411"
     CDS             complement(11910..13535)
                     /locus_tag="OQQ66_002411"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1905314.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13619..14677)
                     /locus_tag="OQQ66_002412"
     CDS             complement(13619..14677)
                     /locus_tag="OQQ66_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1905315.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14680..15318)
                     /locus_tag="OQQ66_002413"
     CDS             complement(14680..15318)
                     /locus_tag="OQQ66_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1905316.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15328..15726)
                     /locus_tag="OQQ66_002414"
     CDS             complement(15328..15726)
                     /locus_tag="OQQ66_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1905317.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15744..16403)
                     /locus_tag="OQQ66_002415"
     CDS             complement(15744..16403)
                     /locus_tag="OQQ66_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCS1905318.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16454..17152)
                     /locus_tag="OQQ66_002416"
     CDS             complement(16454..17152)
                     /locus_tag="OQQ66_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCS1905319.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17225..>17348)
                     /locus_tag="OQQ66_002417"
     CDS             complement(17225..>17348)
                     /locus_tag="OQQ66_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCS1905320.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4410 a         4641 c         4160 g         4137 t
ORIGIN      
        1 aacgaagtgt aatgcttacg atggagtaac aatgaatggt gataattctt ttggatggaa
       61 ccatgaagct acattagata atattcacgc ccagaaatac aataaagctt acgcgggtgc
      121 gaggttaacc gcagaactga aatatttatt gcaagatgtc gaatccttcg aacccaacag
      181 taaatacaca atacacgaag tggttttggg acctggttat ggtactccag actataccgg
      241 ccaaacgata ggatacgttg taacccttcc tgcccaaatg cctaattgct ggtcatctga
      301 actaccaaca atcgatcttt atatcgacca acttcacact gttacaggcg tttctaatgc
      361 cctcggattc atcattgctg cattgctaaa cgcgtattct gatttacccc atgatttgaa
      421 aattggtctg cgttccttat caaattcagc agcaatctac tcgggtttag gtttcgagcg
      481 agtgccgcag gaccgagaca taaggagtga tcgtatgcat ctaacgccag caaaccatcc
      541 tgacttatgg acacaagaga atggtgaatg gatctacttg cgaaactaat acctgacgaa
      601 aaatagaaag caaaacgccg accaatggtc ggcgttttta cgtctcgttg aataacgaat
      661 tattcagcta ctacgtttac gatcactttc gcgaatactt cgctgtgaac ctggaagctc
      721 acttcgtgtt cgccagtggt acgcagaacg ccgttcggca gacgaacttc gctcttagcc
      781 acttcaacgc cagctgcagt tacagcgtca gcgatgtcgc gagtaccgat ggaaccgaac
      841 agtttacctt cgtcgccagc tttagacgcg atggtaacag tttccagtgc attgattttc
      901 tcagcgcgag cattagcagc tgccagaact tcagccagtt tagcttccag ttcagcgcga
      961 cgtgcttcga agaattcaat gtttttcttg gtagctggaa cagctttacc ctgcggtacc
     1021 aggaagttac gagcatagcc cgctttaacg tttacctgat cacctaggct acccaggttt
     1081 gctactttat caagcagaat aacttgcatt accttatcct ctcaaagtcg tattaatgga
     1141 ccgtgaccga ttactgatgg cgatcagtgt acggcagcag ggacaggtag cgagcgcgtt
     1201 tgatagcgcg agccagctga cgctggtatt ttgcacgggt accggtgata cggcttggga
     1261 caatcttacc gctttcggtg atgtagtttt tcagcgtagc gatatcttta tagtcgatct
     1321 cttgaacgcc ttccgcggtg aaacggcaga acttgcgacg acggaaataa cgtgccatat
     1381 ggctagtctc cagaatctat caattcaatc tgctcggcat gcaaaaccat tttgctcagt
     1441 ccgttctttg ccttgtggca tgaaatgaac ccctgaacgg ttatgcgact gccgaccgtt
     1501 atactgtgag taatggcttg gttttcgtgt ccgctaacaa taacgggcat ttggcaccac
     1561 gcctgccggt gaaaaccggc ttcctcctgc acagaacgat gctcaagcac gaactggcag
     1621 tgaggaattc ctgatggact gacctttcga aggggagtcc tgcacacggt gccggacaac
     1681 accagacggt tggtcatcag aaattactct tcagaatccc cagcttcagc atcatcagcg
     1741 gtttcgtttg cgaaatcatc gcgacgctca cggcgctcgt ctttcgcttt aaccatcgga
     1801 gatgcttcgg taacagcgtg cttggtacgc ataaccatgc tgcggataac ggcatcgttg
     1861 aagcggaagg tagtttccag ctcatcgatc acttcctgcg gagcttcaac gttcatcaga
     1921 acgtagtgtg ctttgtgcag tttgttgatc gggtaagcca gctgacggcg gccccagtct
     1981 tccagacggt ggatcttgcc ttctgcacca gtgatggcag cagtgtagcg ctcgatcatg
     2041 cccggaacct gttcgctctg gtcaggatgg accataaaaa cgatttcgta atgacgcatc
     2101 gaattgctcc ttacggatta ttcagcctcc tgtctgggtc agccgaatcc cggggaggca
     2161 aggaacgtgt taaaggtcgg ctgaaaaatg acgcgttata gtacttgtat gccccactaa
     2221 actcaaggcc agccgacaaa taaatctcat ctttcgctca aaagaactaa ctcgctgatt
     2281 tgaagttcat cacattccgt cacagtgttt tttcgaacaa ctgcatcaga aatggtcacc
     2341 atcaacttct ttatcattga ttaaaattaa atcagaagat catcacagag gagagcacac
     2401 aaagtcagcc gtcgaggagg tatctatgtt cagtcgtgtt ttagcccttc tggctgtgct
     2461 tttgctaagt gcaaatacat gggcagccat tgaaattaat aaccaccagg ccagaaatat
     2521 ggacgacgta cagagcttag gcgtgattta tatcaatcat aatttcgcca ctgaaagcga
     2581 agcacgtcag gcattaaatg aagagacaga tgcgcagggc gcaacgtact accacgtaat
     2641 tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc agcgcggata tttatcgcta
     2701 gcaccaggta taaccaacga aacattgcca tagtttgctt tgcccccttc gcagggggct
     2761 tttttatacg gttattcacc ggattgcgac ttccggatct ggctcatgag taatgcgatt
     2821 acgtaagtcg cggcgcataa tttctattat ccagaaccag aaaatatgcc ccaggatttc
     2881 agaggcatat tcgtcaaaag gcaattgagt gagcggcggc gttaaattca gcgccgggca
     2941 aaatataccg tggactgcca ctgtcacaat aagccctgct aatatcccct gccataattt
     3001 cactttcggg aaaatttccg cgacaataca ataccctatc gcaaaaacca gtgagaagat
     3061 gatatgcgtc accatcaccg ggttgataat atgttccgag aatgtataca cagtttgggt
     3121 tgggtcgata cctaaataat cccgtagaaa gatatacggc gggttaaatt catcgcgacc
     3181 accggaaaat gtgcgcggtg gcaaggggac ttccgctccc catttaacaa atgctgaaat
     3241 aattcctgaa agtacaccca caagtaacgc aacgccatag cggcgacgtt gcggctccgt
     3301 tctgacaaac aatccattga tagccataaa aacacctgtt gtttatacga ttttttaact
     3361 gaagcggccc cggaatcaga ccaattacat cccgcaggag gtaataattc cggggcgcac
     3421 tgcttacttc tgcccgtaat aggcgttagg accgtgctta cgcatgaagt gtttattcat
     3481 gaggaagctg tcgatgtgat tcagttgtgg gttaatactg cgcgcaatcc acgccatttt
     3541 cgccacttct tccatcacca ccgcgttatg caccgcgtcg tgggcatctt tcccccaggc
     3601 gaacggcccg tgctgataca ccacaattcc cggcgtatgc agcggctcgg cgtcacccag
     3661 cgtttcgata atcactttgc cggtgttcag ttcatactcg ccctgcacct cttcttcgct
     3721 taatccacgc gtacacggga tgtcgccaaa gaagtagtcg gcgtgcgtgg tgcctaacgc
     3781 cgggatcgcc agccccgcct gcgcccatgc ggtggcatga gtggaatggg tatggacaat
     3841 gccgccgagc gacgggtaac gacggtagag ttcaagatgc gtcgcggtgt cagaagacgg
     3901 gcgatacttc ccttctacca ccttgccgct catatcgacc accaccatat cgtctgcttt
     3961 catggtttcg taggcaacgc cgctgggctt gatcaccacc agcccgcgtt cgcggtcgat
     4021 agcgctgacg ttgccccagg taaaggtcac cagcccatag cgcggcagat ccatgttggc
     4081 ttcaaacacc tgctgtttta gcttttgcat tatgccgcct ccaccatgcc tgctttggcc
     4141 atgcgcgctt tcacccaatc acgcgctttc accacttctg ccgccgggtc ttccgccgtt
     4201 tcgctccaca tctcaatcag gtacggcccg caatagccac tctgtttgag cgtttcgaag
     4261 caacgttcga aatccaccac accttcgcca aatggcacgt ttttgaagac gcccggtttg
     4321 gtgtctttca catgcaccgc gacgatatgc ccgattccgg cctgcaactc catctgcaca
     4381 tcgttgtccc acgccgacag gttgccgata tctgggtaga gctggaacca cgggttgttg
     4441 agatagtgcg cgtaacccag cgccttgctg atggagttca tcaacggata atccatgatc
     4501 tccatcgcca gcgtcacctg cgcgcggctc gccatctcaa cgctctcttt caggccgtca
     4561 cggaaacgac gacgcgtttc gttattggct tcctgatagt aaacgtcata gcccgccagc
     4621 tggatcacgc gaatgccaac gtcctgggcg aactggatgg ctttacgcat aatctccagc
     4681 ccctgcgccc gcaccgcgtc atcttcgctg cccaacggga aacgacgatg ggcagaaagg
     4741 cacatggacg gcacacgcac gccggtttca acaatcgcat tcaccagcgc cagacgctgc
     4801 tcgcggctcc agtcgaggcg cgacaggcgc tcgtcagttt catctaccga catctcgaca
     4861 aaatcgaagc ccaacgtttt tgccagtcgc aggcgttcca gccagcactc cccggcgggg
     4921 agcgcttttt catagatgcc aagcgggatt tgtttggaca acatatccgc tccttagccc
     4981 cacagttcgg cgatggaacg tttgaactga cgcgcggctt caaccggaga agcggcatca
     5041 cggatgctgc gaccggcgat aaacacatga attggaatgc ctttgaacag cggcagatct
     5101 tccagcgcca ggccaccggt gacggtgact ttgaagccca tatcggaaag acgtttgatc
     5161 gcagtgatat cggcttcacc ccacgccacg ccagcggcct gcgcgtcacg gctgcggtga
     5221 taaaccacct gctgaatacc tgcgtcgcgc cactgttgcg cctgttccca ggtccagtaa
     5281 ccggtcagtt cgatctgcac gtcgccgtta aactcttttg ctacgtccag cgcacctttg
     5341 gcggtgttga tatccgcaca acaaatcacg gtcacccagt cagcgttggc ttcgaagcac
     5401 atacgagaaa gaattttgcc cgcatcggca attttggcgt ctgccagcac gattttgtgc
     5461 gggtagagcg ctttcagatc gcgaaccgca cgcacgcctt cgcccacgca cagaatggta
     5521 cccacttcga taatgtcgac ttcttcggca atcaggcgag tggtttcgta ggcgctatcc
     5581 atagtctggt tgtccagcgc gacttgcaac atcggtaatg acatcttcaa ttccttctta
     5641 agctgccgcg ttggtgcggt caatgagatc cagtacttcc tgctcggtac ggcaggcacg
     5701 taaacggtcg aaattttctt catcttcaaa caggttgacg atctgcatga tgcccacttc
     5761 ctggtgcgtg ttggcatcga ccgccgccat ggtgatgagg atatccaccg ggtcgttatc
     5821 ttcgtggttg aactccagcg gctttttcag cgtcaccagc gcaaaaccgg ttttcttaac
     5881 gccctcttcc gggcgaccgt gcggcattgc caggcctggt gcgagcacaa agtacggacc
     5941 gtgctgttca accgcatcca gaatcgcctg gtagtaacgc ggctcgacaa catccgccgc
     6001 caccagcagg tcaacgccga tttttactgc gtcctgccat gtctcagctt cagcctgcag
     6061 gcggatggat ttattctccg ccagcgaatc acgtaatttc atggcgcgtc cttacttcac
     6121 atcctgcggg aaatgcgctt tgatcacttc cagcagtttc gggccaaagt cggcaggaga
     6181 gagcatgttg cgcacgccaa ccacgtattt gttgccggtc acggtgattt cgcccgcaat
     6241 gtgcgtagaa gcgatgatga tatccgcgcc gctcaactcg cttttgtatt cgccaaccgc
     6301 gcagctattt accgtgtggt caatgtttga ttgggttaaa aactggtcca ctttcatctt
     6361 catgatcatg gaactgcctt gtccgttgcc acacacagcc agaatacgta cggtcataat
     6421 caaaactcct tattaagcag actgttctgc cagttgtttt tctgcatctt cttctgcgcg
     6481 cagtgcgcgg ccagcgaaga acatataagc cagtgcaatt acaatgatga cggccataaa
     6541 ggcgataccg atggagaaga agccctgcat catcggcggt gccagaatcg accagtccgc
     6601 catgcccatc caggcgctca taccggtgag tttcaccgcc cagacgcagc caaagatttc
     6661 aatcatcccc atcaccagac atatcttcag cgccgcacgc cagccgccga agtggttagc
     6721 gaacacgccg atggtggcgt tggagaagaa catcgggata aagccgggaa taatcaggat
     6781 cgaggaaccg caggcgacca ggatgccaac cgcaatcagc tgaccgatgg tgccccacat
     6841 aaagccccaa accacggcgt tcggcgcgaa gctatagata gccgcacagt caatcgccag
     6901 aaccgcacca gggatcaggc gctgggagat accgttaaat gcttcagaga gttccgccac
     6961 aaacatgcgc acgccctgag tgatgatgaa gatcgccacc gcgaaggaga aaccagtttg
     7021 caggatgtac accgtccagt tcactttgcc tgccatcgcc tgcacggtgt cgataccgaa
     7081 ggagagtaga atggcaccaa agaagatggt catcacaatc gccgtggaga cgatgttgtc
     7141 gtggaaaata ttcagccagc ctggcagttt gaggtcttca acgctttcct cttttttgcc
     7201 gaggaacggc gcaactttat aggcaatcca tgatgcaaac tgttgctggt gaccgatgga
     7261 gaaaccgcag ccgtccgtca cttcctgagt cggcttgtac atcatattgg aggtaatgcc
     7321 ccagtagagc gaaaccagaa tcgccgtgca gataatggtt gtccacatgg agtagccgaa
     7381 aataaacaac gtaacggcaa tcagccccgc ctgctggaac atgatatgac cagtcagcat
     7441 gatggtacga ataccggtaa tgcgacgcag cagcacgtaa cagatgttca gcgccagcgc
     7501 taacaacacc gcgtaaccta cccagctata ggcatcgccc atgcggtcga tggttgccat
     7561 catcgacgcg taagtatcgg aaattgcgcc gttaatgccg tagacttcgg acattttcgc
     7621 caccaccggt ttgaaggtgc tggtgaggat gccggaccct gcctgcaaca acatgaaacc
     7681 aattatggtt ttaatcgtgc ctttaataat aacgctgaca cttttgcgca gtaggatgta
     7741 gcccagacag gtcacaatac ccagcaacaa cggggcattg gtcatgacct ggttaaaaaa
     7801 cacggtaaag atgttgtaga gacgatctcc tgaagaagag gtaaccgggt attccgcaca
     7861 cccggaatgt tgtgcactca ctctaatttt caaaagcaat cacaacaaga ttatttatga
     7921 ttaaatgtga cgcgacccgc aaataattcc atacggatag agtgggattc acacaacgaa
     7981 acaattattt atttaaattt agttaaattc atttagttaa tttaactata cattccatta
     8041 aatctttcca cactcaaaat caacaacaac ttccctggca aaaagtcaaa aaatggtcat
     8101 tgccttcctg aaaaaatagt gtcaggatta atcaaaacca atcacatatt gattcgaatt
     8161 gaactatcat gaaggtaaat ggcgatgagt aaagtgaaaa gtatcacccg agaatcctgg
     8221 atcctgagca ctttcccgga gtggggtagc tggctgaatg aagaaattga acaagagcag
     8281 gtcgcccctg gcacatttgc gatgtggtgg ctaggctgca ccgggatctg gttgaaatca
     8341 gaaggtggca ccaacgtttg cgttgatttc tggtgcggca ctggcaaaca aagtcacggt
     8401 aacccgttaa tgaaacaggg tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag
     8461 ccaaacctac gcaccccccc gtttgttctt gatccgtttg cgattcgcca gatcgacgcg
     8521 gtactggcga ctcacgatca caacgatcat atcgacgtta acgtcgctgc tgccgtgatg
     8581 cagaactgtg ctgatgacgt accattcatt ggaccgaaaa cctgcgttga tttgtggatt
     8641 ggctggggcg taccgaaaga gcgttgcatc gtggtcaaac cgggcgatgt ggtgaaagtg
     8701 aaagacattg aaattcatgc gcttgatgct ttcgaccgta ctgcactgat caccctgcct
     8761 gccgatcaaa aagcggctgg cgtactgccg gacggcatgg acgatcgcgc ggtgaactat
     8821 ctgttcaaaa cgcctggcgg ctccctgtat cacagcggcg actcccacta ctctaactat
     8881 tatgcgaagc acggtaacga acatcagatc gacgtggctt taggttcata cggcgaaaac
     8941 ccgcgcggta tcaccgacaa aatgaccagc gccgatatgc tgcgtatggg tgaagccctg
     9001 aatgcgaaag tggttatccc gttccaccac gatatctggt caaacttcca ggccgctccg
     9061 caagagatcc gcgtgcagtg ggagatgaaa aaagatcgtc tgaagtatgg cttcaagccg
     9121 tttatctggc aggttggcgg caaatttacc tggccactgg ataaagacaa cttcgagtac
     9181 cactatccgc gcggtttcga tgattgcttc actattgaac cggatctgcc gttcaaatca
     9241 ttcctgtaat ctgtcgttta taccggatag cgtggagaag ttcgcgctat ccggcgtagt
     9301 atttcctggc tatttcaaat atcatctaaa aaaatcaaat tttatcggaa tagctcatga
     9361 ctgaagcaca aagacatcaa atcctcctgg aaatgctcgc acaattgggc tttgtgaccg
     9421 ttgagaaagt cgttgagcgt ctgggaattt cgcctgccac tgcgcgacgc gatatcaata
     9481 aacttgacga aagcggcaaa ctcaaaaagg tgcgcaatgg cgcagaagct attacccaac
     9541 agcgcccgcg ctggacgccg atgaatctgc atcaggcgca gaatcacgat gaaaaagtac
     9601 gtattgctaa agcggcctcg cagctggtta atccgggcga aagcgtagtc atcaactgcg
     9661 gctccaccgc gtttctgctt gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta
     9721 attatctacc gctggcaaat tacctgatcg atcaagaaca tgacagcgtg atcattatgg
     9781 gtggacagta caacaaaagt cagtccatca ctttaagccc gcagggcagc gaaaacagtc
     9841 tctatgccgg gcactggatg tttaccagcg gaaaagggct gaccgcagaa gggttgtata
     9901 aaaccgatat gctgacagca atggcagagc agaagatgct gagcgtggta gggaaactgg
     9961 tggtactggt tgatagcagt aagattggcg aacgcgcggg aatgcttttt agccgtgccg
    10021 atcaaatcga tatgcttatc accggcaaaa atgctaaccc ggaaatcctg caacaactgg
    10081 aagcgcaagg ggtcagcatt ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag
    10141 catctaacgc ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa caggttagca
    10201 gtttatcccg tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg tcggcaggca
    10261 caacgtcatc gtcgaggcca tgccacagca gtagaggtct gtccccaagt tgttccaggt
    10321 ggtttgtcgc ttcccactct gccagcggcg cgataatgtt attgaattca ttctgctgtg
    10381 ctgccgtttc aggtatcagc ggtggaaaca gtgaacgggc gagcgatgta aaatagcccg
    10441 atcccatcat gctggcggta catttcaccg tggggtggcg agcggtaatt cccagtgccg
    10501 tcatcgcgcc catcgacgcg ccaccgactg ccagacggtc atcaagcagc cagttttctt
    10561 cggctattgc cgcacgtaaa gtagtgaatt cctgcatact ttgtagcaag atttgccaga
    10621 attgatttaa ccgccgcgct tcgtcgctac taaaacggct accgtgatcg ggcgcatccg
    10681 gcatgatcac ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta tacaccaggc
    10741 tggatgaagt aaagccgtga taaaaaatta cgcacggtaa cggggtatct ttttgcccga
    10801 caggataagc atgaagaacg ggaatatctg ccagctcgcg tgattctatt tcaatcatct
    10861 gtctctcctt tttgtctggt atgacatgaa gaaatcctta gcacaagttt ctgctggcgg
    10921 tattagttca taatgttgag atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg
    10981 cgtaccaggt aacaattcgg gaacattccc ccaaagttaa attaataagg cactacacta
    11041 tggttagcag gaaacgtaat agcgttattt accggtttgc ctgtttatta ttggtgttga
    11101 tgttaagtgc ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca gtaacgaacc
    11161 atccgcaaga gattcgccgc gaccagacgc aagggttaca gcgaataggt agcgtaagca
    11221 cgatggttcg gggttctccg gatgacgcat tagcagaaat cagagcaaaa gctgtcgctg
    11281 caaaagctga ttattacgtt gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt
    11341 attcacaagc cattttgtac cgtaaataaa tcagacaact tacatagtca gctttacatt
    11401 gctttgcgtc cctatgcgtt tacctgtgca caataaatta ccggctggaa gcctgggcgt
    11461 ttattaccgc gatggaatgc cctgcaacat gtggggaaac gaaaaatgga gctgacgatg
    11521 aaacaattac ttgcttcacc ctcgctgcaa ttagtgactt atcctgcgag cgccacggca
    11581 cagtctgcag aattcgccag tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc
    11641 tcagttagca atatttcagg ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc
    11701 gatgaacaag gtgcgtcatg gtaccgcatt attaccatgt acgaagacca gcagcctgat
    11761 aactggcgcg tacaggcgat actgtatgca tgagtgtcat ctacagcaaa tgaacaatgc
    11821 cggattcggc gcgagcgcct tgtccggcct acaattttca catatttcat tgagttaatc
    11881 gaaaccagcg tcgcatcagt cgtatacgct tacacacaca ctccccccgt cgcccgcagc
    11941 aataaatcat tctggatctg ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc
    12001 tgacaccacg cctgcgccat tggcggagaa gcatatttca acatttgcgc accgcagccc
    12061 agcaggaata gctgatgagt aatctctcgc cccagttctt cagctggctt acgcagctgc
    12121 tgctgtaaac gacgaaccgc gcgatcaaaa tagcgatcct gccctttcac ttccacaaat
    12181 gcttccgaca ataagtcgta tacgcccgct tgcttattga gaacgcgcaa cacatccagg
    12241 cacataatat tgccggaacc ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc
    12301 ggcagctcgc tctcctcgca ataaccaatg ccgcccagca cctccatcgc ttcggccaca
    12361 aacggcatac ctcttttgca gatcacaaat tttgccgcag gcgtaaataa acgcgcccac
    12421 agggcttctt tggcatcggc acgccggtcc cacgctcgcg caagacgaaa cagcaacgcc
    12481 gtttgccctt caagctgaag tgccatgcga cttaagacat gacacataag gggctgttgg
    12541 atcaatggat taccaaaaac atggcgttga tgtgcatgat aaatcgccag cgaaaatgca
    12601 cggcgcatca tggcatggct acccagggcg caatcaaaac gcgtcatccc acccattttc
    12661 aggatcagac gaattccttc cccttcctgc cccaacaacc agccaatggc atcctgaaac
    12721 tccacttcgc aactggcgtt agagcgatta cccagcttat ctttcagccg ctcgaggcga
    12781 atcgcgttgc gttgcccatc aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc
    12841 gtagtctgcg ccagcaccag atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc
    12901 cccaccagcc gataagagcc atcttccaga cgctctgcac gggtggtgtt gctcatgaca
    12961 tcggaaccgc cctgcttttc cgtcattccc atgccaatca acaaaccgcg tttttgccca
    13021 cctggcaata agagagaatc gtagcgatcg ctcagcagcg gcgtggtcca gtcctgaaac
    13081 ggcgtgggta acatctgtaa caacaatggc gtggcggcaa aggtcatcgt tattggacat
    13141 aacgacccag cctcaacctg cgcatgtaac ataaaacgcg ccgcgcgcgc cacaaatgcg
    13201 ccggagcgag cgtcttcttc ccaggcaaga ttgtgcaccc gattggtaca taacgcctgc
    13261 atcagcaggt gccaggcggg gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg
    13321 tagcgcaata attcaggcgg attcacattc gccagccgcc ccagttcaag agattcagcc
    13381 gttcctaact gctgaccaat actggctaga aaatcgctat cccagccagc accttcacgc
    13441 gttaccgctt cacagagcgc gccatcagac aggtataagt tgctgttatt taatggtata
    13501 ggttgattaa aaacggtgtg agtttgccag tgcactgtgt ctccctccat caatggcagt
    13561 cacccttagt atggtcactg ccatggattc ctgacagaaa gagattgcta aaacattctt
    13621 atccagcaat gtaatgcgga acgaaacgtg aggtgtcttt ggtgatcagc gtgttatctt
    13681 cacggatccc cattccgcac gcttcatcat cgacaatcca gctaccgatg agtgtgtagc
    13741 tatcgccaaa tcgcggcaga ggttgaaacg cctggtagat catcggttca tcggcgtaat
    13801 caccatcagc gtggtcaacg acgttattct gaccgtcgaa aatggtgacg tttccgcctt
    13861 cgcgtgagta gatcggttta cgcacatagc tttcgccagc ggcaatctgc ggtttttcgc
    13921 cctcaaacca ggatgcaaga aggttaggat gaccaggaaa gaagcgccaa agcagcggca
    13981 ttagcccttt attactcaag atacttttcc ataacggctc cacccattgc tcgcgacgct
    14041 tgcgcagcag cggaccgtta tcgtcacgca tcatccactc cagcggatac agcttaaatg
    14101 cacgctggat gacattatca tcaagatcgg tcagtacgcc gccgacgccc aaaccgagat
    14161 cttcaatgta gataaaccgc gactcctgcc ctgcctgctg ggcgcagtcc tgcaagtaca
    14221 gcacggtact cctgtcttca tcggtgtcct gacagcagca aaaataaaac ggttcccgac
    14281 tgtaaagctc actaaagcgc gaaatcaggc gttcctgaat ggcgttgtac tccagcagct
    14341 tcaccggcgc attgccacac caggcaaaat ccatccggcc atacagcgaa ggatcgcgtg
    14401 cgcgccagct ttcggcgatc acatcccagt acaacggcgg gatcgccagt tgcgtcagga
    14461 tctcttcgtc tttaaccgcg cgatccacca cctcaaggca catctgatgc agttccgcag
    14521 tcggtttttc gatctgctct tcaatctggc gcagagtaaa acggtaagcc cgactctcgt
    14581 cccaatagat ttcattgtcg atgatatgaa agtcgaaacc gttgttagcg gcgatctggt
    14641 ccagatcccg tcgcacagga acgttgtgtc tcagcatgat tagcctcccc aatgcccacg
    14701 ggcgctggaa gaacgaccat agccgccgcg cgaaacggtt gacgcttttt tggtggtaaa
    14761 gccgcctgaa gagtaagact cttttttgcc agaaccggag cgccaggagt aatcaccgga
    14821 agtgttgagc cagacggggc gggaagcaaa tgaggatccg ccgctgttat agacgaacgg
    14881 ctcatcgcga tctttacgaa taacccggct taacaaaaat ccagaaacga ccgggatcca
    14941 actctgttca acattgtcgt agtagcaatt ttcgtactta gactgacaat tctgctgagt
    15001 catattcttc ggaacatcgg cataaaatgc cgctttggcg ttgttccagc cacgcgcgca
    15061 aatgtcggca ttattaccgt catcaataca atcctgcact gtcgaataaa acgttccgtc
    15121 gccgtcgtta tcgacatcgc cactatcgcc acaacctttc aatacaaaaa aagcggcacc
    15181 gcccattagc gccaacgtta agtatttttg tgcgtagcga ttgcgacgcg gttcaaaagg
    15241 attattcggt ctaccaatgc gactgatcgc tccgtgacca attttactgt tatttcttga
    15301 tttgcgtttt ctggccatga tcgtccatta ccatgtcata caagctgcgt taaaaatacc
    15361 gccagccagc gcggcggttc ccataaacat tcctgctgcg gtattgtgat taataatttt
    15421 ttcgcttaat gcgggcatat aaagtctgac accagcaaaa acgagtaact gaatcaccag
    15481 tgcaatcccg ccccaggcga aatagtctgg aatactcacc gcattgattg ccgcactgga
    15541 taaggggatc acgtaaccca acaatgtacc gctgaatgcc agtgacaccg cggtattatt
    15601 gtttttgatt aattgccatt cgttgtgcgg tgtaattttg gagtagataa acagaaaaat
    15661 aatcaccata gccacgccaa taaaaaaata ggcactaaag gcaagaagtg aatccagtat
    15721 atgcattgta aacgtccttg taattaacca ataatatgaa gtgatgtcag cgggatatcg
    15781 acacccaacg cgcgcgaaaa taaccactct ggctcgccga gatcgttaaa agattcctca
    15841 ccatttaaca gtaaatattc gtaagtatct tcggttactt gccgctggta gcccatggta
    15901 aaattatgga cctcccattt ggcatggttt tgattttcta ctttttccag catgtagacg
    15961 ggttcgatat ttcccggttc ttcgctgtta aaaaatcgct gccagcgttt ttcctgccag
    16021 tttaaggtca ttgcccccat cgttttggcg ttgatcgcct cgcgccagtg actctcttta
    16081 ctgataccgt agctctcttc atagacaaag agcttgatat catcaatgtc atcaatatct
    16141 tcgccgccgg tggtattgat ttgcagaaat tcatcgcctg aagtgtagta acggaaaatc
    16201 tggctaccgc ctcccagatc gatgcggctg acagcggcta ccgtaaattc ctcacccggt
    16261 agcgcaatca gcaattcatc ttccagcaaa cgaaacgcta acgtgtcgag cgtaaagcca
    16321 ctattgagat gaagtcccag cggaccgcga gcgatagcag gcttattatc cttgccaaac
    16381 agacgctgga aaaaaccaga catattttat ccttaaaata gttgccagcc ttttagcgag
    16441 gctggcaaaa aaattactcg ccctgttggc gttgcagtct tgctaataca tcctgggctc
    16501 tacttttatt gctaccgcca attccggctt ccgccagctt ttcgtcaagg tcgcgaccgt
    16561 ctgcgacttt ctccaactgt gcggcagcat ccaggcgagc ctgacgttcg gcctgacgcg
    16621 tttgcaggcg ttttaaggat tctgccgctg tcgaaacgct ggaagatgcg ccaacggtag
    16681 aggttgttac agcctgttgt gcacgctgca tggcttcagt ggctttaacg acttccattt
    16741 gctgctcaaa ctgagcgata cgctgcgctg tcgctgtaac tgctttttcc acgccatcac
    16801 gggagacttc cagattcgac aacacttgct cttcagcggt aatgagattc tcaaggcgcg
    16861 cgatttcttc agcaacttcg ttaatcaacg acggattaac gttcttgctt aacgcttcca
    16921 gcgcacgggc ttccagactg gctttgcgct cacgtaaatc tttcagctta tcgtgactta
    16981 atttcacccg cgccagcaga tcaacgcgag attttccggc tttatcgagt tcagctttag
    17041 cgtcacgaat atgctgttcc agcatgcgca cgccctgggt ttcttcaatg gattcttccg
    17101 cctgggagat aaacgatttc cccagcgtaa ataaactttt taaaattccc ataaataccc
    17161 gtccttgtaa ttagtgtgaa tattcttctg taatttcagc cagatccaat gcgttatcta
    17221 ccagctaggt tatctcaagc aggatatctt caagagaaga tttaagcgat aacgccccga
    17281 aaacaatgta atactcttcc tgttgcacgc tggagattcc taccgatgac aacggcatca
    17341 tcttctga
//
LOCUS       DAJVTY010000082        17048 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVTY010000082 DAJVTY010000000
VERSION     DAJVTY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17048
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.082"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(254..535)
                     /locus_tag="OQQ66_002418"
     CDS             complement(254..535)
                     /locus_tag="OQQ66_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1905321.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(798..1907)
                     /gene="apbC"
                     /locus_tag="OQQ66_002419"
     CDS             complement(798..1907)
                     /gene="apbC"
                     /locus_tag="OQQ66_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1905322.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            2039..4072
                     /gene="metG"
                     /locus_tag="OQQ66_002420"
     CDS             2039..4072
                     /gene="metG"
                     /locus_tag="OQQ66_002420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1905323.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4213..8007
                     /locus_tag="OQQ66_002421"
                     /pseudo
     CDS             4213..8007
                     /locus_tag="OQQ66_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
     gene            8017..11649
                     /locus_tag="OQQ66_002422"
     CDS             8017..11649
                     /locus_tag="OQQ66_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCS1905324.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALKEPPARPGNPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMQWLSTQPQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     VFVKPCDNSEAALFALRLLYEQGHSELLQTVANRWDRADMWPALEKILTQNPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTNDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYNLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11710..12027
                     /locus_tag="OQQ66_002423"
     CDS             11710..12027
                     /locus_tag="OQQ66_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905325.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12334..13422
                     /locus_tag="OQQ66_002424"
     CDS             12334..13422
                     /locus_tag="OQQ66_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1905326.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13433..15712
                     /locus_tag="OQQ66_002425"
     CDS             13433..15712
                     /locus_tag="OQQ66_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905327.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15705..16841
                     /locus_tag="OQQ66_002426"
     CDS             15705..16841
                     /locus_tag="OQQ66_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1905328.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16838..>17048
                     /locus_tag="OQQ66_002427"
     CDS             16838..>17048
                     /locus_tag="OQQ66_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905329.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQC"
BASE COUNT         4328 a         4389 c         4303 g         4028 t
ORIGIN      
        1 gtctgtatct cttggaaaca aatttctcac atcaaaaaag ttgccgcaat gtaaacacat
       61 tgttttattg atttaaaaca aattaaccat tgcaatataa atacaaaaaa tatttaagag
      121 tattaactat ttatcgcatc tatcaattaa tgtagattta tgtaaatggt attgttgata
      181 tcaataaaaa agccgaaatc atttatataa atgatttcgg ctttctaaat gcaattaaaa
      241 tcaggttgcc aaattaatcg tagcgattta acacgattcg acgtttgcta tcgctacctg
      301 caacaaccac ttctgtcgtt acacctttga cgggcgaaga aaacagatga cgggtgtcaa
      361 cattatacgt taactgacca caatgctgtt gaggttgacc attaaccata gccatactgc
      421 atgttggggg actgatcctg ccattaatcg ctaatgtggc agtctctgaa gagaatgcgg
      481 gagaagccaa tccataggca agaaaaataa ttcctgacag ccaatactta ttcattgaac
      541 gttatccctg tagtaaaggt tatgcctgga tagaatgagt gcataacaaa ctatagctgt
      601 acatccacta cacagccacg aaggatgata atgaagcatt gcctgtatga tcaatcgact
      661 ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt attaaataag tgttcattaa
      721 tgcggcaaaa actaatacac cgcatcaatg taacatctct gcggtgtatg aagaataacc
      781 agaatggtta ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat gacttcaccc
      841 tgccagtaga gctgagctgc aacgcggtca gccagctggc ggtagatagc ggtaaattcg
      901 ctctccggac ggctgataac cgttggcgta cctttatcga gatcctcacg taaggagatg
      961 tgtaacggca tctgccccag cagctgtgta tggtattttt cagccagttt ctctgcacca
     1021 ccggtaccga aaatcggttc atggtgacca cagttactgc aaatatgcac actcatgttc
     1081 tcgacaatac cgagcaccgg aacctcgact ttctcgaaca tcacaatgcc ttttttcgca
     1141 tcgatcagcg cgatgtcctg cggggtggtg accacgactg caccggttac tggaatgttt
     1201 tgcgccagcg tcaattgaat gtcaccggtg cctggcggca tatcaagcac cagatagtcg
     1261 agatccggcc acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc catcggtcca
     1321 cgccacacca tcgcattgtc gtcagtgacc agataaccaa ttgaattggt tgccaggcca
     1381 tgagacataa tcggtgccat atgggtaccg tccggcgaag ttggacgctg attttctgcg
     1441 cccaacattg ttgggattga tgggccgtag atatcggcgt ccagaatgcc gactttcgcc
     1501 ccttctgccg ccagcgccag tgccaggttt accgccgtgg acgatttacc cacgccgcct
     1561 ttaccggaac tcaccgcgat aatgttcttc acaccgttaa ttcccggctg attcttcacg
     1621 cgtttcagag tggctatgtt atgcgacagt ttccagtcga tagctttcgc cccggtgatg
     1681 cgcagcagtt cggcgctaca ttgctctttt aactcttcga acgcactatg ccagacaaac
     1741 ggcataacca gttccacatg caacgtgtcg tccatccagg cgacatggtg caacgctttc
     1801 agcgtggtga ggttatgctt cagggttggg tgctgaaaat tggccagtgt cccggcgacc
     1861 attgcgcgca aggcttctgg tgatttggcc tgggattgtt cgttcatccc gactcctttt
     1921 ttgtatagat aaaccatcag ttgatagttt acctgaagaa tatagagaag tacttactta
     1981 acattttccc atttggtact atctaacccc ttttcactat taagaagtaa tgcctactat
     2041 gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat
     2101 ccacctcggc catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat
     2161 gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc cacggtacac cgatcatgct
     2221 gaaagctcag cagcttggta tcaccccgga gcagatgatt ggcgaaatga gtcaggagca
     2281 tcagactgat ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga
     2341 agagaaccgc cagttgtcag aacttatcta ctctcgcctg aaagaaaacg gttttattaa
     2401 aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt
     2461 tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt
     2521 ctgcggcgcg acctacagcc cgactgaact gatcgagccg aaatcggtgg tttctggcgc
     2581 tacgccggta atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat
     2641 gttgcaggca tggacccgca gcggtgcgtt gcaggagcag gtggcaaata aaatgcagga
     2701 gtggtttgaa tctggcctgc aacagtggga tatctcccgc gacgcccctt acttcggttt
     2761 tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg ctggacgcac cgattggcta
     2821 catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct tcgatgaata
     2881 ctggaagaaa gactccaccg ccgagctgta ccacttcatc ggtaaagata ttgtttactt
     2941 ccacagcctg ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgtccaacct
     3001 gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg tccaagtctc gcggcacctt
     3061 tattaaagcc agcacctggc tgaatcattt tgacgcagac agcctgcgtt actactacac
     3121 tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac ctggaagatt tcgttcagcg
     3181 tgtgaatgcc gatatcgtta acaaagtggt taacctggcc tcccgtaatg cgggctttat
     3241 caacaagcgt tttgacggcg tgctggcaag cgaactggct gacccgcagt tgtacaaaac
     3301 cttcactgat gccgctgaag tgattggtga agcgtgggaa agccgtgaat ttggtaaagc
     3361 cgtgcgcgaa atcatggcgc tggctgatct ggctaaccgc tatgtcgatg aacaggctcc
     3421 gtgggtggtg gcgaaacagg aaggccgcga tgccgacctg caggcaattt gctcaatggg
     3481 catcaacctg ttccgcgtgc tgatgactta cctgaagccg gtactgccga aactgaccga
     3541 gcgtgcagaa gcattcctca atacggaact gacctgggat ggtatccagc aaccgctgct
     3601 gggccacaaa gtgaatccgt tcaaggcgct gtataaccgc atcgatatga ggcaggttga
     3661 agcactggtg gaagcctcta aagaagaagt aaaagccgct gccgcgccgg taactggccc
     3721 gctggcagat gatccgattc aggaaaccat cacctttgac gacttcgcta aagttgacct
     3781 gcgcgtggcg ctgattgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct
     3841 gacgctggat ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt ctgcttaccc
     3901 ggatccgcag gcactgattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa
     3961 aatgcgcttc ggtatctctg aaggcatggt gatggctgcc ggtcctggcg ggaaagatat
     4021 tttcctgcta agcccggatg ccggtgctaa accgggtcat caggtgaaat aatccccctt
     4081 caaggcgctg catcgacagc gccttttctt tataaattcc taaagttgtt ttcttgcgat
     4141 tttgtctctc tctaacccgc ataaatactg gtagcatctg cattcaactg gataaaatta
     4201 cagggatgca gaatgagaca ctttatctat caggacgaaa aatcacataa attcagggca
     4261 gttgagcaac agggaaacga gttgcatatc agttggggaa aagttggcac caaagggcaa
     4321 agccagataa aaagtttttc agatgctgcg gcagcggcaa aagcggagct taagctgatt
     4381 gcggagaagg tgaagaaggg gtatgtggag caagcgaagg ataattcttt gcaaccttcc
     4441 caaacggtaa cgggctctct caaggtagcg gacttatcca ccattattca ggaacaaccc
     4501 tctttcgtag cagaaacccg tgcgcctgac aaaaatacag atgctgtttt accgtggctg
     4561 gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac acaccacgtt aagtcatcgc
     4621 cgctttcccg gagttcctgt tcagcaagca gacaaattgc cccaattacg tcgcttagcc
     4681 tgtagtgtgt cgcaacggga taataaaaca gccacatttg acttcagcgc ctgttcttta
     4741 gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg acggcctgaa aacaacacag
     4801 ttaccatcac cagtaatggc tgtactcacg gcacttgaaa tgaaatgcac aagatataaa
     4861 gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg gtctggaata tgctactgat
     4921 gtaataatac accttcaaca gattgatatt gaatgggatt atgcgaataa tgtcattatt
     4981 attctgccgt ctggcattgc acctagctac ttggagcaat attccagatt tgaatgacgc
     5041 ctacgtaaac atttatcact gacggaagag tctctctggc aaaaatgtgc acaaaaactt
     5101 attgccgcaa ttccacatat tccagaatgg cggcaaccat taattgcttt gttattaccc
     5161 gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa aaaattaccc
     5221 tcgcttgagt ggttaaaaat agtggcgact gatgagcaca ttcttgcctc attagaaaaa
     5281 tatcacgaac catatgccat ttttgatgat tactattgtg gtgcgatatg gtcagccacc
     5341 gtattacagg agcaaggtgt tgcagccctg ccccgatttg ctccctatgc cgcaagtgac
     5401 tactgcgccg atgtgttgcg tcatatcaat catccgttcg cattgacact gcttatacgt
     5461 gtagccgggc aaactaaacg ctgtcacgat cggatgacga aagccattgc tgcgttccca
     5521 catgcagcaa tggcggcact gacggaactt cttgggcaaa aagaagagaa cagttggcgc
     5581 attatgctaa tgacaatgct tatctcacaa ccagcactgg cagaacaggt cattccctgg
     5641 ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc aacaactgac acagccctca
     5701 aaccatgcca gcgccgatct actgccagcc gtagtagtct cccctccctg gctttcgaaa
     5761 aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcattga gccaatctgt
     5821 tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta ttcaaaacac
     5881 atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg ttttcaacga
     5941 cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg gctaaatgaa
     6001 gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg gcattattgg
     6061 cagttgggga ttttgacaac attgccgctg gagaaagcag taaaagcatg gaatgccctt
     6121 accctatctc cacataccga taccgaatac tccatgttac attttggact caaagggtta
     6181 cctgggttag taaactcact tgcacgctat ccacaagaag ccttgcccat cacgaattac
     6241 ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct tcaacaaact gaaaacgcta
     6301 cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac atgcccttac cggcctgctg
     6361 cctgcggcgc tcggcaaagc cggtgaagca caggataacg cccgcgctgc cttgcgtatg
     6421 cttaccgaaa acggtcatca gccattactg caagaaatcg cccgacgtta taaccagccg
     6481 gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc ccttagataa tcacccgaca
     6541 aaaatcccca ctcttccggc cttttatcag ccatcgctct ggacgcgccc ggtattaaaa
     6601 gcaaatgccc aatcactgcc agatagcgcc ctcctccacc tcggtgaaat gctccgcttc
     6661 cctcaggaag aggctctgta tccgggatta ttgcaggtga aagacgtctg ttccgccgac
     6721 tcactggcgg gatttgcctg ggatctgttt accgcctggc agaccgctgg cgcgccgtcg
     6781 aaagagagtt gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa
     6841 ctgacgccat taatacgcgc ctggcctggt gaatcccagc ataaacgcgc caccgttggg
     6901 ttggatattc tcgctgctat cggtagtgat atcgccctta tgcagcttaa cggcatcgcc
     6961 cagaaactga aattcaaagc attacaggag cgggcaaaag aaaaaattgc cgacattgcc
     7021 gagagccgcg aactcacggt ggcggagctt gaagatcggt tagcaccgga tctcggtctg
     7081 gatgataacg gttcgctgct gctggatttt ggcccacggc agttcaccgt cagctttgat
     7141 gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc gcctgaaaga cctgcccaaa
     7201 ccgaacaaaa gcgatgatga aacgcgggcg aacgatgcgg ttaaccgcta caaattgctg
     7261 aaaaaagatg cgcgtaccat cgccgcccag caggtagcaa ggctggaatc cgccatgtgc
     7321 ctgcgccgcc gctggtcgct ggaaaacttc cagctcttcc tggttgagca tccgctggtt
     7381 cgtcacttaa cccgccgtct gatttggggc gtttatagcg ccgaaaacca gctactggct
     7441 tgctttcgcg tagcagaaga taacagctcc agcaccgctg acgatgatct tttcaccctg
     7501 ccggaaggcg atatctctat cggcactcct cacgttctgg aaatatcacc aacggatgct
     7561 gccgcctttg gtcagctttt tgccgactac gaactgctac caccgttccg ccagctcgac
     7621 cgtaacagct acgccctgac agaagccgag cgcaatgcca gtgaactgac ccgctgggca
     7681 ggcagaaaat gcccgagtgg tcgggtcatg gggctggcga ataaaggctg gataaagggc
     7741 gaaccacagg atggaggctg gatcggatgg atgatcaaac ctttgggtcg ctggtcgtta
     7801 atcatggaaa tcgatgaagg ctttgcggta ggcatgtcgc cagccgaact cagcgctgag
     7861 cagctcttaa gcaagctgtg gctatgggaa ggcaaagcag aaagatatgg ctgggggagt
     7921 aattcaacac aggaagcgca gttctccgta atcgatgcca tcaccgccag cgagctaatt
     7981 aacgatattg aagccctgtt tgaataagga aagagcatgg acaaggaatt accgtggctg
     8041 gcggataacg cccaactgga actgaaatat aaaaaaggca aaacgccgct cagtcatcgt
     8101 cgctggccgg gcgaaccagt gtccgttatc actggaagtc tcatccagac attgggtgat
     8161 gaattgctac aaaaagctga gaagaaaaaa aacattgtct ggcgttatga gaatttttca
     8221 ctggagtggc agtccgccat cacgcaggcc atcaacttga tcggcgaaca caaaccctca
     8281 atcccggccc ggacaatggc ggcgctagcc tgtatcgcgc aaaatgacag ccaacagttg
     8341 ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga ctgaggtggt gattgcacgc
     8401 cagtttattg cgcggtgtta tgagagtgat cctctggtag tgacattgca gtatcaggac
     8461 gaggattatg gctatggtta tcgctcagaa acctataacg aattcgatct ccgactgcgt
     8521 aagcatctct ctctggcaga ggaaagctgc tggcagcgtt gcgccgacaa actcattgcc
     8581 gcactaccag gaataaccaa agttcgccgc ccttttattg cgctgatcct cccggaaaaa
     8641 ccagaaatag ccaatgagtt ggtaggcctt gaatgcccgc gaactcattt tcattctaag
     8701 gagtggttaa aagttgttgc taatgacccc acagcggtga gaaaactcga acactactgg
     8761 agccaggata tatttagcga tcgagaagcc agctacatgt cgcatgaaaa ccacttcggc
     8821 tacgcggcct gcgccgccct tttgcgcgaa caaggactgg cagccattcc gcgcctcgcg
     8881 atgtatgccc ataaagaaga ttgcggcagt ctgctggtac aaattaacca tccgcaagtc
     8941 atccgcacct tgctactggt ggctgataaa aacaaaccca gcctgcaacg tgtagctaaa
     9001 taccataaaa acttccccca tgcgacgctc gccgcactgg cagaactgct ggcgttaaaa
     9061 gaaccaccag cccgcccagg taatccaatc atcgaagaca aaaagctgcc tgcacagcaa
     9121 aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc tgatggcatc gcagccacaa
     9181 ctggcagaag aggtgatgca gtggttaagt actcaacccc agtcagtgct gaagagttat
     9241 ttatcggcac cgcccaaacc ggttattgat ggcaccgata acagcaatct gccagaaatc
     9301 ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag ctccacgtct tgatttggca
     9361 ccgctcgaat taactccgca agtttactgg caaccaggcg aacaagagag gcttgccgcc
     9421 actgagcctg cccgttattt cagcacggaa tctcttgcgc aacgcatgga acaaaaaagt
     9481 ggacgagttg tattacagga actgggtttt ggggatgatg tatggctgtt tctgaattat
     9541 atactccccg gaaaactgga tgctgcacgc aattcactca ttgttcagtg gcattactac
     9601 caggggcggg ttgaagagat cctgaatggc tggaactccc cggaagcaca attagcagaa
     9661 caggcgctcc gcagcggtca catagaagcg ttaattaaca tatgggaaaa tgacaactac
     9721 tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt tattggcaca gttgccgcgt
     9781 gagatggctt tgaccttctg gctgcgtatc aatgagaaaa agcatctgtt cgcgggtgag
     9841 gactattttc tcagtatcct cggattggat gcgctaccag gtctgctgtt ggctttttca
     9901 catcgtccaa aagaaacatt tccgttaatt ttaaatttcg gcgcaacaga actggccctg
     9961 cccgttgccc gcgtctggca ccgttttgcg ggccagcgta atctggctcg ccagtggatt
    10021 ttacaatggc cggaacatac ggctactgca cttattccac tcgtctttgt taaaccctgc
    10081 gacaacagcg aagcggcatt atttgccctt cgtttactgt atgaacaagg acatagtgaa
    10141 ttactgcaaa cggttgcaaa ccgctgggat cgcgctgata tgtggccagc cctggaaaaa
    10201 atacttaccc agaacccgat ggaaatttac ccggcacgca ttccaaaagc ccctgatttc
    10261 tggcatccgc aaatgtggtc caggccgcgc cttatcacta ataatcaaac tgttaccaat
    10321 gacgctctgg aaattatcgg cgaaatgctg cgctttaccc aggggggacg tttttatagc
    10381 gggctggaac aactgaaaac gttctgccag ccacaaacgc tggcagcttt tgcttgggat
    10441 ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag acaactgggc atttctggcg
    10501 ttaagtctct ttggtgacga aagcacggca cgggatctga cgacacagat cctcgcctgg
    10561 ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga acatccttac cctgatgaat
    10621 aatgatatgg cgctgataca gctgcatcat atatcgcaac gggctaaatc ccgcccctta
    10681 cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa atcgcgggct aagccaggaa
    10741 gagctagcgg acagattagt cccaaccctg ggccttgatg atccgcaggc gttgagtttt
    10801 gattttggtc cccggcagtt taccgttcgc ttcgatgaaa atctcaaccc ggttatcttt
    10861 gatcagcaaa acgttcgcca gaaaagcgtt ccccgtttgc gcgccgatga cgatcaactg
    10921 aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgctac tcaggtgagc
    10981 aaaaacctgc tcccgcgtct tgaaacggcc ctacgtacca cccgacgctg gtcgctggca
    11041 gattttcatt ctctgtttgt taatcatccc tttacccgtc tggttaccca gcgattaata
    11101 tggggggttt atccggcaaa tgaaccgcgt tgtttactca aagcctttcg tgtggccgca
    11161 gagggggagt tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga cgctctgatt
    11221 ggcattgccc acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt
    11281 gccgattacg aaattatgcc gccttttcgc cagttgtcgc gccgcacggt gctgctcaca
    11341 cctgacgagt caaccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg
    11401 caacttatgg gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat
    11461 aacctgggtg agtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt
    11521 gatagcaaag cgctaatgag cttccgttct cttcgagtgt accgtgacaa taaatccgtc
    11581 acttttgccg aacttgatgt gtttgatttg agtgaggcgt taagcgcacc tgacgtcatt
    11641 ttccattaaa acacagcggg cagtgatgca actgcccgtt atcaacacga cttaccagcg
    11701 gggaaagcga tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta
    11761 cctgaagatc cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt
    11821 tccgtctatc gactggagcg attccggcta cgtccaacat ttcatcaacg tgatcgagaa
    11881 gatgctgacc cgctaataaa tgatgcgttg atttatataa gagatgagtg tattgatgag
    11941 cggaaattac gaggtgaatc acctgaaact gtaatagcaa tttttaatcg tgaactacag
    12001 aatatattca accaagaaat agaataatat actctaaata attcaaattg gtccgatccg
    12061 gcgcaacgtc ccaatggcct ggattataaa tctcattatc ttaattgcaa cggggtccag
    12121 ccgtggttat aattcgttcg ttggaacacg atgttgcaca ggctgtggtg ttaggcctga
    12181 aaatagtaaa taaaagaacc tgcctcacca gcaggttttt ttatttactg tgatctgctt
    12241 tccagatatt tttcgctcaa acaactaatg cgccaaacat ttattgcgcg taaaatatcg
    12301 tttatttcat taatacattt cagggatgaa tatatgtcac cgcagaataa tcatctgcag
    12361 cgtccgcctg ctgctgtgtt atacgccgat gaactggcaa aattaaaaca aaatgataac
    12421 gcaccttgcc cgcccggttg gcagttaagt ttgcctgcgg cccgtgcttt tatccttggc
    12481 gacgaagcgc aaaatatcag ccgtaaagtg gtgattagcc cctccgctgt cgaacgtatg
    12541 ttagtcactc ttgctaccgg ccgcggtttg atgttggtgg gggaaccggg taccgcaaaa
    12601 tctcttcttt ctgaattact ggcaaccgct attagcggcg atgccgggtt aaccatccag
    12661 ggcggggcat ctaccaccga agatcaaatc aagtatggct ggaactacgc cctgcttatc
    12721 aatcatggtc cgtcaacgga agcgctggtc cctgccccgc tttatcaggg aatgcgcgac
    12781 ggcaaaatcg tccgtttcga agagataacg cgtacaccgc tggaagtgca ggactgtctg
    12841 ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga agccagtcag
    12901 ctctatgcgc gagaaggctt taatattatt gccactgcca atacccgcga tcgcggcgtc
    12961 aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg aaacggtttt tccgattatg
    13021 gatttcgccc aggagttgga actggtcgcc agtgcttcgg cgcgtttgct ggcccatagc
    13081 ggtattccgc ataaagtgcc tgacgcggta ctggaattac tggtccgcac cttccgcgat
    13141 ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac tgacagcgat tatgtccacc
    13201 gctgaagccg tgaacgtggc ccatgctgtg ggcgtccgcg cctggttttt agcgaatcgc
    13261 gcgggggagc ctgccgacct ggtggaatgt attgccggaa ccatcgtcaa agataacgaa
    13321 gaagatcgcg cgcgtctgcg ccgttacttc gaacagcgtg tcgccacaca taaagaagct
    13381 cactggcagg cttattatca agcccgccac cgcctgccgt gaggaaagat gcatgagcga
    13441 gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg tgaaatcgtt
    13501 aatcgaaagc cagcggccac gatacgtgtt gattgaaggc ccggctgatt ttaatgaccg
    13561 ggtagacgaa ctgtttttag cccaccagct tccggtagct atttacagtt attgccagta
    13621 tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat tttcgccgga
    13681 gtggcaggcg ctacaagccg cacgtcgcat tcaggcacaa acttacttca tcgatttgcc
    13741 ttgctgggcg cagagtgaag aagaggacga ttcgcctgat acgcaagatg aaagccaggc
    13801 cttactgctg cgtgccaccc gcatggataa cagcgatacc ctgtgggatc acttgttcga
    13861 agatgaaagc cagcaaactg cattaccctc tgcgctggcg cactattttg cccaactgcg
    13921 gggcgacgcc tccggcgatg cgctcaatcg tcagcgcgaa gcctttatgg cccgctggat
    13981 tggatgggcg atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg gctggcacgc
    14041 tccggcactg gcaaagatgt ggcgcgaatg ccctcagaaa attaacaagc cagaattgcc
    14101 ctcgctggca gatgccgtta caggttgtta tctcacaccc tacagtgaaa agcgccttga
    14161 tgtgctggca ggataccttt caggaatgcc tgccccggta tggcaaaact ggtgctggca
    14221 gtggggcttg cagaaggccg gtgaacaact gctaaaaact atccttaccc gtttgcgcca
    14281 gcacaaattg cccgcttcta ccgcggatat ggctgccgct catctgcatg cgatggcgct
    14341 ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg ccatagcagg
    14401 ctcgctgatt aaagaagccc tgaacgcgcc gttgccgtgg agctatcgcg gcgttattca
    14461 tcccgatacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg acggattcgg
    14521 taaacttgcc ccttctacgc cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga
    14581 acgtaccgca atctcccttc cggcggagct taccttaaat cgctttaccc ccgatgggct
    14641 ggcgcaaagt caggtgttac atcggctggc aatactggag atccctggga ttgtacgcca
    14701 gcagggaagt acactgacac ttgcaggcaa cggtgaagaa cgctggaaat taacccgccc
    14761 gcttagccag catgcggcat tgattgaggc cgcctgtttt ggtgccacac tccaggaagc
    14821 cgcacgcaat aaattagaag ccgatatgct ggacgcgggc ggaatcggca gtatcaccac
    14881 atgtcttagc caggcggcgt tagcgggtct ggcgtccttc agtcaacaat tactggagca
    14941 actcacacta ttaatcgccc aggaaaatca atttgccgaa atgggccagg cgctggaagt
    15001 gctttatgcc ttatggcggc tggatgaaat tagcggtatg caaggcgcgc agatattaca
    15061 aacgacgtta tgcgcgacta tcgatcgcac gctgtggctg tgtgaatcta acggcagacc
    15121 ggatgaaaag gagtttcacg ctcacctgca tagctggcaa gcgctttgcc atattctgcg
    15181 cgatctacat agcggcgtta atttacccgg cgtttctctt tctgcggcgg tagccttact
    15241 ggagcgacgc agtcaggcaa ttcatgcccc ggcgctggat cgcggcgcgg ctcttggcgc
    15301 actaatgcgt ctggaacatc ccaacgccag tgccgaagcg gcgctgacga tgctggcgca
    15361 gttatccccg gcacaatctg gtgaggcgct gcacggtttg ctggcgctgg cccgccatca
    15421 actggcctgt cagccggcat ttatcgccgg tttcagcagt catttaaatc aactgagtga
    15481 agccgatttt attaacgccc tgcccgattt acgcgcggcg atggcctggc taccaccacg
    15541 agaacgcggg acgctggcgc atcaggtgct tgagcattat caactggcgc aacttcccgt
    15601 ttcggcgctg caaatgccgt tgcattgtcc accacaggcc attgcacatc atcaacaact
    15661 cgaacagcag gcactggcat cgctgcaaaa ctggggagtt ttccatgtct gaactgaacg
    15721 atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa
    15781 cgacgctttg tgggctggat gacaacgccc ggcagataga ccacgcgctg gagtggctgt
    15841 atgggcgcga tcctgaacgg ctccagcgtg gtgaacgttc cggtggatta ggtggctcaa
    15901 atctcaccac ccctgagtgg atcaacagta ttcacacgct gtttccgcag caggtgattg
    15961 agcggctgga aagcgatgcc gtgctgcgct acggcattga agatgtggtg acgaatctcg
    16021 acgtgctgga acgtatgcag ccttctgaaa gcctgctacg cgctgttttg cacaccaaac
    16081 atctgatgaa ccccgaagta ctggctgccg cccgccggat agtgtgccag gttgttgaag
    16141 aaattatggc tcgactggca aaggaagttc gtcaggcttt ttctggtgtc cgcgatcgcc
    16201 gtcgccgttc atttattcca ctggcgcgaa actttgattt caaaagtact ctgcgcgcca
    16261 acctgcaaca ctggcacccg caacacggca agttgtatat cgaatccccc cgctttaaca
    16321 gccgcattaa acgccaaagc gaacaatggc aactggtctt actggttgat caaagcggct
    16381 cgatggtcga ttcggtgatc ccctctgcgg tgatggcggc ctgtttgtgg cagttacccg
    16441 gcattcgtac ccatctggtg gcgtttgaca caagcgtcgt tgatctcacg gcagacgttg
    16501 ccgatccggt agagttatta atgaaagtac agttgggcgg cgggaccaat atcgccagtg
    16561 ccgtggagta tggtcggcaa cttattgaac aaccagcgaa aagcgtcatt atcctcgtga
    16621 gcgattttta cgaagggggt tcatcatcat tactgacgca tcaggtgaaa aagtgtgtcc
    16681 agagcggcat caaagtgctg ggactggcag cgctcgatag cacagcaaca ccttgctatg
    16741 accgcgatac ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc atgacaccgg
    16801 gcgagctggc atcatggctt gcggagaatc ttcagtcatg aattcactac gtccggaatt
    16861 attagaactg acaccgcagg ccctgacggc gttaagcaat gccggttttg ttaagcgcag
    16921 tcttaaggaa ctggaaaatg gcaacgtccc ggagatcagc catgagaacg gcgctttaat
    16981 cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggccaggcac tgaaagaggc
    17041 tcagtgca
//
LOCUS       DAJVTY010000083        17035 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVTY010000083 DAJVTY010000000
VERSION     DAJVTY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.083"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..672
                     /gene="csgG"
                     /locus_tag="OQQ66_002428"
                     /pseudo
     CDS             1..672
                     /gene="csgG"
                     /locus_tag="OQQ66_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(736..1227)
                     /locus_tag="OQQ66_002429"
     CDS             complement(736..1227)
                     /locus_tag="OQQ66_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1905330.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1329..1883)
                     /gene="ycdY"
                     /locus_tag="OQQ66_002430"
     CDS             complement(1329..1883)
                     /gene="ycdY"
                     /locus_tag="OQQ66_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1905331.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1907..2644)
                     /gene="ycdX"
                     /locus_tag="OQQ66_002431"
     CDS             complement(1907..2644)
                     /gene="ycdX"
                     /locus_tag="OQQ66_002431"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1905332.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2699..3637)
                     /gene="ghrA"
                     /locus_tag="OQQ66_002432"
     CDS             complement(2699..3637)
                     /gene="ghrA"
                     /locus_tag="OQQ66_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS1905333.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3871..3958
                     /locus_tag="OQQ66_002433"
     tRNA            3871..3958
                     /locus_tag="OQQ66_002433"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3905..3907,aa:Ser,seq:gga)
     gene            4003..4139
                     /locus_tag="OQQ66_002434"
     ncRNA           4003..4139
                     /ncRNA_class="other"
                     /locus_tag="OQQ66_002434"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4230..4916)
                     /locus_tag="OQQ66_002435"
     CDS             complement(4230..4916)
                     /locus_tag="OQQ66_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1905334.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(5497..6285)
                     /gene="phoH"
                     /locus_tag="OQQ66_002436"
     CDS             complement(5497..6285)
                     /gene="phoH"
                     /locus_tag="OQQ66_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1905335.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6907..8178)
                     /gene="efeB"
                     /locus_tag="OQQ66_002437"
     CDS             complement(6907..8178)
                     /gene="efeB"
                     /locus_tag="OQQ66_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1905336.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(8184..9311)
                     /gene="efeO"
                     /locus_tag="OQQ66_002438"
     CDS             complement(8184..9311)
                     /gene="efeO"
                     /locus_tag="OQQ66_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1905337.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(9369..10197)
                     /locus_tag="OQQ66_002439"
                     /pseudo
     CDS             complement(9369..10197)
                     /locus_tag="OQQ66_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            10233..10535
                     /locus_tag="OQQ66_002440"
     CDS             10233..10535
                     /locus_tag="OQQ66_002440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905338.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPGDINDYS
                     RVERWDCLQRTMCSCRMPRKRHIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(10866..12374)
                     /gene="putP"
                     /locus_tag="OQQ66_002441"
     CDS             complement(10866..12374)
                     /gene="putP"
                     /locus_tag="OQQ66_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS1905339.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(12533..12742)
                     /locus_tag="OQQ66_002442"
     CDS             complement(12533..12742)
                     /locus_tag="OQQ66_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905340.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            12797..16759
                     /gene="putA"
                     /locus_tag="OQQ66_002443"
     CDS             12797..16759
                     /gene="putA"
                     /locus_tag="OQQ66_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1905341.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(16799..>17035)
                     /locus_tag="OQQ66_002444"
     CDS             complement(16799..>17035)
                     /locus_tag="OQQ66_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HCS1905342.1"
                     /translation="KALIDEKSALIAGWVKSGKLAPIDPQHLIFIIWASTQHYADFAP
                     QVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         4278 a         4590 c         4197 g         3970 t
ORIGIN      
        1 aacattcagg acgaaaccgg gcaatttaaa ccctacccgg caagtaactt ctccactgct
       61 gttccgcaaa gcgccacggc aatgctggtc acggcattga aagattctcg ctggtttata
      121 ccgctggagc gctagggctt acaaaacctg cttaacgagc gcaagattat tcgtgcggca
      181 caagaaaacg gcacggttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca
      241 aatattatgg ttgaaggttc gattatcggt tatgaaagca acgttaaatc gggcggtgtt
      301 ggggcaagat attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg
      361 aacctgcgtg tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag
      421 acgatacttt cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg
      481 ctggaagggg aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct
      541 atcgaaacag gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg
      601 caaaataaag cagaacggca gaatgacatt ctggtgaaat accgccatat gtcggttcca
      661 ccggaatcct gacagataaa aaagccggaa ttatcatccg gcattttact gtttaagcga
      721 aagcgtgagt gcccctcacg ctttattttt gatttcctgc tcacggtgag ccgtctttgc
      781 actacgcgcc gccagccaca ggccgctgtt tttcattgcg taaccaaata ccagcccaag
      841 cgccagcgaa ggtaacacca gtttccagtc accctgcccg gcaaacgtcg cgcatgcgcc
      901 tataaacgtc cccggaacaa atgaaagcag cagctgtttg gcctgaatac acatcagaaa
      961 cgccacaata ccggttatga cataaccgag aatttccaga tgtggtgcca gtgcactacc
     1021 gtaaataatg accatcgccc acaccacgcc actaagcagg gttgcagcgg agatcgccag
     1081 ccctttcagc ccaccttgcg ggcaggcaaa gtaggccgta cagcccagga agcccgccca
     1141 gcttagtaag cccagggaaa cagccaccca tccccagata ccggagagaa tgcctgttgt
     1201 gattgcaata gagagaagta tgttcatgcc gcgcatgata gcagaaaacg cggccatgaa
     1261 tgagatcgag cacacatttt atgcaacaac aaattatctg ttgcagttat gagtgacgat
     1321 gatcacattt actcttcaga atcttcttcc agctcgtccc acattgcact aatggcatca
     1381 cgggttagcg gtgccatggt gcgccagaaa ggcgtggttg catgggcctc cactttgccg
     1441 aggaacgcac cacaccaggg taacagatac tcactgaaca gtgtttccag tgcttcgctc
     1501 tcatcttccg ttgactgatc ttccatccag gaagccgcaa gcagcaatgt gccgatgtga
     1561 tcggctggcg tatccgccag tggcatccct cgttcggaaa gaaaagcgcg cacttccgct
     1621 tccgttgcgt cctcaaccca tgcgctacga tatggcggca cggcacattc atcgccgata
     1681 aacaacgcat tgtaatcggc agagacttgc gccatatcac agcttttctg taaacgcgtc
     1741 agtaactcat cctgctccag tggccagttc gcagccagtt tcccctcacg aatcagggta
     1801 aacagcggca ccagtaaagg atcttgcggt tggcggtaat agagcgaacc cagcacacga
     1861 cagaggatag aaaactcgtt cattaatata tttccattac tagtaattaa aggtctgcaa
     1921 attccgcaat cggtgccata ccgcgagatt caaggaagtt cagtaagcgg cgcggagaaa
     1981 cattcaaaat gcgctctggc ggaaaatcta ccgcgtcgag gattttaaga cactcttcaa
     2041 attcccccat ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa cctccagcat
     2101 cacgtaccgc tgcggctacc gcacgacagt tgtcttcact gcccttacgt gagtgtaaaa
     2161 atgaagaatt attgatttcc agcgccacct gatgtttcgc ggctgcttca gcaacggctt
     2221 tcacatctat ttcatatttg ggatttccgg gatggcttat tatatgcaca ttgccgctgg
     2281 cgatagtggc gatcatcgct tgtgtattgg tcgctttgtc atgtggtgca aaaaccggct
     2341 catgaaaacc ggcaataatc agatccagcg agtcaaacat tttaccgctg caatcaattt
     2401 caccatcaac atttttaatg ttagcttcga tgccgcgcag gatccctacc ccatcaacca
     2461 ctcgcggcca gatacgcatg ttaatgaagt gccagtgatg cggagcatct tccatatccg
     2521 ggccatgatc ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg gcaatgtaat
     2581 cactcaatgt gctatatgca tgtgtgctgg caacggtatg catatgaagg tcgacgggat
     2641 acataactct ctcctgtatt caattcccgc caaggatagc aggaatcctg atgctttatt
     2701 agtagccgcg tgcgcggtcg acttgcccgc agaccctctc ccctttttcg agctgggcaa
     2761 tggtgcgaga aatgtactcc acagcttcag cgggacgggt aatcgcggcg acatgtggtg
     2821 ttatcgtcac gcgtggatgt tgccagagcg gactttcagg cggtaagggt tcacgattaa
     2881 aaacatccaa cattgcgcct ttaactttgc cgctatccag cgccgcgagc aggtcatctt
     2941 ccacaacatg aacaccacgc gccaggttga ggagatacgc gccatccggt aatttttcga
     3001 gtaattgttg attaataatg ccgacggttt caggggtatt cggtaacaaa ttaatcaata
     3061 cccgacattg gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt tgcacgccag
     3121 gccacgattt acgggttcga ctccagcaac gcagcggaaa gcgccaggtt tgcagactct
     3181 gagcaacttt actgcccagt acgcctgcgc ccaaaatgcc gatggtaaaa tcttcccaat
     3241 gatattcagg cagcggttgc caatgcgaac tattttgctg gatgcgataa tcgtcaaaac
     3301 gtcgaaacca atgcagcacc tgactgacag catattcctg catttgctcg cccataccag
     3361 tatcttccag gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt gcctgtagct
     3421 tgctcaaaat agaatcaaca ccggccccga gtgcgaacac cgctttaaga tcgcgccctg
     3481 ccagcatttc aacaggagga tgccagacta aagcataatc agcagagtca ttatctccgc
     3541 ttttccatgc tctgactctt gcctgaggaa tagctttgcg cagtgcctca atccaccatt
     3601 gggtatcgaa cgttgggtga taaaagatga tatccatact gactcccgaa aagcgttgtg
     3661 cgaaatttat tcgcacttat cgttatgatc tacaaaggcc accagcataa caaatccgtg
     3721 gtcggtggca aaaaaagcag atttcgctta ttaaaaccac acattgattg aagtttgaat
     3781 aaacgcgcga ttttttcaaa aagtttgttg acctcaggtc atgatttccc taaattagcg
     3841 cccgttccag caagacagga acaacaattt ggtgaggtgt ccgagcggtt gaaggagcac
     3901 gcctggaaag tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccata
     3961 tttaaagaag agctcgtacg aaagtacggg cttttttttc gtatattgca cacaccgggg
     4021 ggatgagaag ccccgaccgg ggttcgacaa ctggcgacag ccagttggac agaccgtgaa
     4081 cgcagtgagc gggctgcccg cagggcgagc gaagcgagtc aatccccccc tcaccgccat
     4141 ctttaaagaa gagctcgtac gaaggtacgg gctttttttc gtatattgca cacaccgggg
     4201 ggatgagaag ccccgaccgg gatacttagt taatgcaccg gttcactctt tgtcgccgcg
     4261 actaatgggc gaattttgtc acgagtctcc cagaccggag gaatttcctc caatttagtg
     4321 acagaagctc gtacaaaagg cacccgtgca agagtcataa ttgtatccaa ccaacggtca
     4381 cgaataatgc gatccgccct ttcatgactc gggtcatcca gctcaataac acagactatt
     4441 ttattgttct tctttgaaac cagaacataa tccattgcca tgccattata aattgcaaag
     4501 aaacgctttc tttcttcttc gctaaactca ggttgttctg gaaagttgat caagcgcccc
     4561 agagagacct ggcaatggat atgacaataa tgtccaaacc atttttccag attatcgaaa
     4621 tacatttttt cacaacgagt gattaaccac tcttttcttt ccaataaaat accttcggca
     4681 accattccct gaaataactc atctcgctct tgccaacgag agtcacgata ggttttctgc
     4741 ggtatatagc tccttgtcga cgccttagcg gcaggggaag ttgcagatga agggacgggt
     4801 tgcgatttag cgatgattac cgatgaggca acttttcgga gtgtcaattt tctggcaaca
     4861 gcaaaaccgg cgataaatgc aattgtcaaa caagttacaa aagccataaa gctcataatt
     4921 aattttcacc tatccatgtt gattatattt cattaccaat gttactcgct gcagcgtgtt
     4981 acatgccaac aatgcaacga tcaatatata gttagtatca tgttttgatt attacaacca
     5041 tccctattta gccattgcgg cttgccttcc acaaaaaaat aacaacacac aatattagca
     5101 aagaattaat taagattaac cttacattta taatattatc cctaaagaat aaatcagagg
     5161 aatatattcg gtgttaaaca gtcagtcaaa gtatagccca ttgttgatta aacctcataa
     5221 gcaaaattgg caccagatct cctgtacagg ttgaatggcc gaaaaaagat aggcagatgc
     5281 tcattttttt ggcttctgga agaaaaactc tccgcgctgg cagtattaca ctggtctgat
     5341 gctatgacga aaaatgcctg gtaagagtat ctaaaccaga ctccagttca gcttatcaac
     5401 acgctcaaag tcgcccccct gaacgctaac ctgaagtgaa aatctgttga aataaaacaa
     5461 agcccggttc gcccgggctc tgcaccgata acacacttaa ctgtatgcat gcagcgtacg
     5521 ttggcaaagt gccgaacgta cgcaatcctc ttttccgaac cggacgatcc caaccatttc
     5581 atcttcttcg aaacgttcca gcgcgtcact taatccggag cacacgccgc gaggcaaatc
     5641 gcattgcgtg atatcaccgt tgacgataac cgtcacgttc tccccgaggc gggttaaaaa
     5701 cattttcatt tgcgcggcag tcacattctg cgcctcgtca agaatgacga ctgcattttc
     5761 aaaggtacgt ccacgcatat aggcgaacgg cgcgatttcc accttcccta tttccggtcg
     5821 caggcagtac tgcataaagg aagcccctaa gcgccggacc agcacgtcgt agaccgggcg
     5881 aaaataggga gcaaactttt ctgcgatatc accaggtaag aagccaagat cttcatcggc
     5941 ttgcagaact ggacgggtga cgataatcct gtcgacatcc ttatgtatca gggcctctgc
     6001 cgcttttgct gcgctgatcc aggtttttcc gcacccggct tcgcccgtgg cgaatatcag
     6061 ctgcttactc tcaatagcct tcagatagtg caattgcgct tcatttcgcg cgaggatggg
     6121 cgaggtatcg cgactgtcgc gggccatacc aatggcttct acgccgctca tctgcacaag
     6181 cgaggtgacc gattcttctt cacgctgctt atggctgcgc gaatcccgtc tcagcacacg
     6241 ttttgcctcg cgacgagctt tgatcactgc tttttgtctt cccatggaga gcaccttgag
     6301 ttgtttgtat tcatcacacg cgccgttggc agcgcgatta tgcgcacgaa catcagaggg
     6361 ttggcttcct tgtaagccat agtttgcttt tggataaaat gccgaaaaac ggctacgcgc
     6421 accgtttacg gcgtcggtaa cacatgaaaa gaaaggatga ggttgaaaat gcaaagtgac
     6481 gagatgacta ccggaggaga aaactccgcg agtggtggcg cgttgattat ctaaaacatg
     6541 tccagtacag gacgttacca tccgcgatct ccatagtgac tgactatcac tgccgggaac
     6601 ttccgctgct acttaataag tacaacagat ctcgcatttt attgcaacaa tatatttact
     6661 tatatttaac tataaaacac tatttcagtg acattagttt ctactggaaa gatgacagag
     6721 tgatgacagt gatgaaaaaa gctgtgtgct ttcagcagga tttgcatcag gcatcagtgc
     6781 acgatcgccg gatgcggcgt gaacgcctta tccagcctac caaacccgca ctgattgtag
     6841 gcatgataag acgcgcaagc gtcgcatcag gcatctgcgc acaaatgcct tatccgccta
     6901 aaaacattaa acccgcaata acgcgcttcc gagataatcg ttcgcgtcct tcactcccgg
     6961 cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt
     7021 gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag
     7081 caaccccata tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg
     7141 cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc
     7201 cagcgcgatc accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg
     7261 cattcccagc ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag
     7321 cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc
     7381 accgattgtc cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa
     7441 cttatcattc tggctatcgg gattggcagt gccgtcttta aagcccagca aattaatcgg
     7501 cgtctctttg cctttactac gcgctgcgtg atcggaaata aacccttccc gcttccagcg
     7561 cacgctgagc aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga tcaccgtgtc
     7621 ctgggtgttg gcgcaaattt gtagcaacac atcaccatgg cataacgccg catccagcga
     7681 gtcgttgggg aaacgcgtca tcttctgcaa ctttttcggc atctgtggcg caaggccaaa
     7741 ccgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat
     7801 gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg tttctggtgc
     7861 cgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac aagcgaaaca accgttcaag
     7921 atcggcttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg
     7981 cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg
     8041 tgaaagcgta cccggcgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc
     8101 cagcgccagc gcacctatca ctttcagtaa acgtcggcgt gacggttcat tcacgccgtt
     8161 ttcatcttta tactgcataa cgcttaatcc agtcccagca caccgcgaag ttgcgccaga
     8221 tcttccgcct gcgcagtaat cggtcctttc agtgcattcc ggtcggcatc ggtcaatttg
     8281 tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc gaccttttta
     8341 aagttggcat cgacttttgc cagcagttcc gggttggctt tttgcagttg tggacgcagc
     8401 aaatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag atcggtgtgg
     8461 ctgtagcgat cttcttcacc gctaattttg ctggctgcca cttcctcaat cagtccggct
     8521 gcgccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt ttgcaaatcg
     8581 accacatctg tataaagctg ctcagcgtac tgatccatcc ctttggtggt gttgtcgcca
     8641 aacaatgctt tttccagacg gtggaaacca gtgaattttg ggtcggcggc tttttgctcg
     8701 taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc agcaatcggt
     8761 tcaatgcgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt ttcgatatcg
     8821 ccagctttaa tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt ggtttccgcc
     8881 atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc atcactttgc
     8941 gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt agtcagcaga
     9001 ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg gctaaaacca
     9061 ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc ccactccagc
     9121 gccttctggc tatggttctg aataatgaac tgtgttttcc cggcgttaac cgtaatggtc
     9181 atcggttcgc actgcttatc cgtcacggtc actttgacct gcggcacatc agcggcgtta
     9241 gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt acggcagaag
     9301 ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa gagtttgcgt
     9361 cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag agcaaatgcc
     9421 accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct cggcgcttcc
     9481 tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga gagcaccgca
     9541 ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg aaatgcgcga
     9601 atggcaccag ctgccagccc tgcggcgacg aagagaataa acaggctggt ccatttaaaa
     9661 aatgcaccaa gattgaggcg aataccgccc cagtagagca gaaagccgag caccacggct
     9721 gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg ttgaaatgcc
     9781 gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa gaccatcatc
     9841 accagcgccc agccatgatg atttccacgc tacaatgcgc tatcgactgc ctgttccagt
     9901 tgcacttacg ttgcgcgaca ctttgcgcat ccagaaaacc atccaggtaa ggatcaccac
     9961 ggcgatcacc gccacgatac cttcaaacag ttcctgttct ttttgcggaa attcgccggt
    10021 ggtttcgtta atgaagatcc ccaggcccag gcacaacgca gcggcaagca acacgccaat
    10081 ccacatcaca ccaatccatc ggcctcgctg ggtacgctta agatagctgg caatcaaact
    10141 gacaatcagc gcggcttcaa gtccttcgcg caacataatg agaaacggaa caaacatgcc
    10201 acccaccctt aaacgttttt ctgagtcaat ctatgcaaag aaaagtaaat cgcagtgata
    10261 gcgattatca ttacggtaaa gaaaaataca agcggaatgt ttataaaatg atgttggaat
    10321 gtaaacaaag agaggtaatg ccaggggata taaacgatta ttccagggta gaaagatggg
    10381 attgtttgca aagaaccatg tgttcttgcc ggatgccgcg taaacgccat atccagccga
    10441 caaaactgtc cagactcaat gcatcagagc ttccatgtaa aactgataag cgggtgtatc
    10501 aagcaatttc gtccatccgc gattatcaaa tctaactttt tgcccggaac ataataacag
    10561 ctcgggtgtt ggtggcgtca aaatcgctga agcgttcccc gaaggccggg acagcccaca
    10621 tggatgtggg ctgagggcgc gtcttacagg acgttacctc gcgcccgacc cggtagccga
    10681 aggggataag ctgaaggtac cgcgcagcgg cgattttgtt cgccagagcc cgggggtgca
    10741 cagggggcgg cggcgattgg ccgccccctg cgcgctccct gcaccattca atttatatgg
    10801 cgaggcacat taagggagcg caacccgctg ctgaagccaa acaaaaccgc cgcaggctaa
    10861 gtcccttagc tttcctgcaa ccgtgacggc ggagccgaat gatagtgcgc atcggcctcg
    10921 gcaaagcgtt tttgcatcgc cgctgacggc gctttaccca gcaaactaaa cactacaatc
    10981 ccgatactgc cgaagatgaa gcccggaata atttcgtaca gtcccagcca gccgaactgc
    11041 ttccagacga taaccgtcag cgcaccgatg atcatccccg ccagtgcacc gttacgcgtc
    11101 atgcgtgacc acatcaccga gaacagcacc accggaccaa acgccgcgcc aaagcctgcc
    11161 cacgcgtagc tcactaagcc cagcacgcgg ttttccgggt ttgccgccag cgcaatcgcc
    11221 accagcgcca ccaccagcac catcacacgc cctacccaca ccagctcttt ctggctggca
    11281 tgtttacgca gaaacgcttt gtataaatct tcggtaatcg cactggagca caccagcagt
    11341 tggcaactta aggttgacat taccgccgcc agaattgccg acagcagaat cccggcaatc
    11401 cacgggttaa acagaatttg cgccagttcg ataaacacgc gctcggcgtt ctggtttacc
    11461 gcgcctgcta ccgccgggtg ctcattaaag taagcaatcc caaagaagcc gacagccacc
    11521 gcccctgcca ggcagaggat catccaggtc atactgatac gacgcgcatg gacaatgctg
    11581 tggtgagaat ccgccgccat aaaacgcgcc agaatgtgcg gctgcccgaa gtaacccagc
    11641 ccccaaccca tcagtgagat aatggcaaca aagttcagac ctttgagcat atcaacgttt
    11701 tcgatgctct tttgtttgat cacttccagc gagtcaccaa agccaccgac actgataatg
    11761 acgataaccg gtgtcaggat cagggcaaaa atcatcaggc tggcctgtac agtgtcagtc
    11821 cagctcaccg cgaggaaacc gccaataaag gtgtaaagga tcgtcgccgc agctcccgcc
    11881 cacagagccg tttcgtagct catgccaaag gtactttcaa acagacgcgc gcctgccaca
    11941 atgcccgaag cgcaataaat ggtgaagaac agcaaaataa ccagcgcgga gataatgcgc
    12001 aaaatgcggc ttttatcttc aaaacgcccg gtgaaataat ccggcaatgt taaggcgtta
    12061 ttattgtatt cggtatgcac acgcaaccgc ccggccacca gcttccagtt gatccacgcg
    12121 cctaatgtca ggccaatggc gatccagctt tcggaaatcc cggaaagaaa aaccgcgcca
    12181 ggcaacccca ttaacagcca gccgctcata tccgacgcac ccgccgataa tgccgtcaca
    12241 aatggcccaa gactacgacc gcccagaata tagtcgtcaa agtttttcgt tgatcgccag
    12301 gcgataaacc caatcaatat catgccaaag atatagacac aaaatgtcac caacatcggt
    12361 gtgctaatag ccatctaaag tctccaaaaa attattatcg gcaatgtcga aacttgccgt
    12421 tatatctgca accggaacgg ggtaacagag tttatttttt accagggcga ccgtatcctg
    12481 ccggaagcgc tggttattca caatcgattt aacacaccat ttacattaaa ttttagtgct
    12541 cagcgacact atttttcatc aggttgcact ctctcacatt ttttgcggtt gcacctttca
    12601 aaaatgttaa ctgccgcaga gaaaaagtct gagttatttt tttaatccct gtcatatcaa
    12661 tttcttttat taacatttca ttcattttta agcttgctac gcaggtcaca tttaacatgg
    12721 ttgcacaaag ttgcaacatc atggatattt cacgataacg ttaagttgca cctttcagaa
    12781 caacaggagt aatggcatgg gaaccaccac catgggggtt aagctggacg acgcgacgcg
    12841 tgagcgtatt aagtctgccg cgacacgtat cgatcgcacg ccacactggt taattaagca
    12901 ggcgattttt tcttatctcg aacaactgga aaacagcgat actctgccag agctacctgc
    12961 gctgctttct ggtgctgcca atgagagcga tgaagcaccg actccggcag aggaaccaca
    13021 ccagccattc ctcgactttg ccgagcaaat tttgccccag tcggtttccc gcgccgcgat
    13081 caccgcggcc tatcgtcgcc cggaaaccga agcggtttct atgctgctgg aacaagcccg
    13141 cctgccgcag ccagttgctg aacaggcgca taaactggca tatcaactgg ccgataaact
    13201 gcgtaatcaa aaaaatgcca gtggtcgcgc aggtatggtc caagggttat tgcaggagtt
    13261 ttcgctgtca tcgcaggaag gcgtggcgct gatgtgtctg gcggaagcgt tgttgcgtat
    13321 tcccgacaaa gccacccgcg acgcgttaat tcgcgacaaa atcagcaact gtaactggca
    13381 gtcacacatt ggtcgtagcc cgtcactgtt tgttaatgcc gctacctggg ggctgttgtt
    13441 tactggcaaa ctggtttcca cccataacga agccagcctc tcccgctcgc tgaaccgcat
    13501 tatcggtaaa agcggtgaac cgctgatccg caaaggtgtg gatatggcga tgcgcctgat
    13561 gggcgagcag ttcgtcacgg gtgaaaccat cgcggaagcg ttagccaatg cccgcaagct
    13621 ggaagagaaa ggtttccgtt actcttacga tatgctgggc gaagctgcac tgactgccgc
    13681 agacgcacag gcgtatatgg tttcctatca gcaggcgatt cacgccattg gtaaagcgtc
    13741 taacggtcgt ggcatctatg aaggtccggg catttcaatc aagctgtcgg cgctgcatcc
    13801 gcgctacagc cgcgcccagt atgaccgggt aatggaagag ctttacccgc gtctgaaatc
    13861 actcaccctg ctggcgcgtc agtatgatat tggtattaac atcgatgccg aagaggccga
    13921 tcgtctggag atctccctcg atctgctgga aaatctctgt ttcgagccgg aactggcagg
    13981 ctggaacggc atcggttttg ttattcaggc ttatcaaaaa cgctgcccgt tggtgatcga
    14041 ttacctggtt gatctcgcca cccgcagccg tcgccgtctg atgattcgcc tggtgaaagg
    14101 cgcgtactgg gatagtgaaa tcaagcgtgc gcagatggac ggccttgaag gctatccggt
    14161 ttatacccgc aaggtgtata ccgacgtttc ttatctcgcc tgtgcgaaaa agctgctggc
    14221 ggtgccgaat ctaatctacc cgcagttcgc gacgcacaac gcccatacgc tggcagcgat
    14281 ttatcaactg gcaggtcaga actactatcc gggtcagtac gagttccagt gcctgcatgg
    14341 tatgggcgag ccactgtacg agcaggtcac agggaaagtt gccgacggta aacttaaccg
    14401 accgtgtcgt atttatgccc cggttggcac atatgaaacg ctgctggcgt atctggtgcg
    14461 tcgcctgctg gaaaacggtg ctaacacctc gtttgttaac cgtattgccg acacctcttt
    14521 gccactggat gaactggtcg ccgatccggt cactgctgta gaaaaactgg cgcaacagga
    14581 agggcaaact ggattaccgc atccgaaaat tcccctgccg cgcgatcttt acggtcacgg
    14641 gcgcgacaac tcggcagggc tggatctcgc taacgaacac cgtctggcct cgctctcctc
    14701 tgccctgctc aatagtgcac tgcaaaaatg gcaggccttg ccaatgctgg aacaaccggt
    14761 agcggcaggt gagatgtcgc ccgttattaa ccctgcggaa ccgaaagata ttgtgggctt
    14821 tgtgcgtgaa gccacgccgc gtgaagtaga acaggcgctg gaaagtgcgg ttaataacgc
    14881 gccaatctgg tttgccacgc ctccggctga acgcgcggcg attttgcatc gcgctgccgt
    14941 gctaatggaa agccagatgc agcaactgat tggtattctg gtgcgtgaag ccggaaaaac
    15001 cttcagtaac gctattgccg aagtgcgcga agcggtcgat tttctccact actacgccgg
    15061 acaggtgcgg gatgatttcg ctaacgaaac ccaccgtcca ttagggcctg tggtgtgtat
    15121 cagtccgtgg aacttcccgc tggctatttt caccgggcag atcgctgccg cactggcggc
    15181 aggtaacagc gtgctggcaa aaccggcaga acaaacgccg ctgattgccg cgcaagggat
    15241 cgccattttg ctggaagcgg gtgtaccccc tggcgtggtg caattgctgc caggtcaggg
    15301 ggaaaccgtg ggcgcgcaac tgacgggtga tgatcgcgtg cgcggggtga tgtttaccgg
    15361 ttcaaccgaa gtcgctacgt tactgcagtg caatatcgcc agccgcctgg acgctcaggg
    15421 tcgccctatt ccgctcatcg ctgaaaccgg cggcatgaac gcgatgattg tcgattcttc
    15481 agcactgacc gaacaagtcg tcgtggatgt gctggcctcg gcgttcgaca gtgcgggtca
    15541 gcgttgttcg gcactgcgcg tgctgtgcct gcaagatgag attgccgacc acacgttgaa
    15601 aatgctgcgc ggtgcaatgg ccgaatgccg gatgggtaat ccgggtcgcc tgaccaccga
    15661 tatcggtccg gtgattgata gcgaagcgaa agccaatatt gagcgccata ttcagaccat
    15721 gcgtagcaaa ggccgtccgg tgttccaggc ggtgcgggaa aacagcgaag atgcccgtga
    15781 atggcaaagc ggcacctttg tcgccccgac gctgatcgaa ctggatgact ttgccgaatt
    15841 gcacaaagag gtctttggtc cggtgctgca tgtggtgcgt tacaaccgta accagctacc
    15901 agagctgatc gagcagatta acgcttccgg ctatggtctg acgcttggtg tccatacgcg
    15961 tattgatgaa accatcgccc aggtcactgg ctcggcccat gttggtaacc tgtatgttaa
    16021 ccgtaatatg gtgggcgcag tggttggtgt gcagccgttc ggcggcgaag ggttgtccgg
    16081 taccgggccg aaagcaggcg gtccgctcta tctctaccgt ctgctggcga atcgcccgga
    16141 aagtgcgctg gcagtgacgc tcgcgcgtca ggatgcagag tatccggtcg atgcacagtt
    16201 gaaagccgca ttgactcagc cgctaaatgc actgcgggaa tgggcagcaa atcgtccaga
    16261 attgcaggcg ttatgtacgc aatatggcga gctggcgcag gcaggaatac aacgattgct
    16321 gccggggccg acgggtgaac gcaacacctg gacgctgctg ccgcgtgagc gcgtgttgtg
    16381 tattgccgat gatgagcagg atgcgctgac tcagctcgcc gccgtgctgg cggtgggcag
    16441 ccaggtactg tggccggatg acgcgctgca tcgtcagcta gtaaaggcat tgccatcggc
    16501 agtcagcgaa cgtattcaac tggcgaaagc ggaaaatata accgctcaac cctttgatgc
    16561 ggtgatcttc cacggtgatt cggatcagct tcgcgcattg tgtgaagccg ttgctgcgcg
    16621 ggatggagca attgtttcgg tgcagggttt tgcccgtggc gaaagcaata tccttctgga
    16681 acggctgtat atcgaacgtt cgctgagtgt gaataccgct gccgctggcg gtaacgccag
    16741 tttaatgacg atcggttaaa caggtagccg gaggatgtta caccctcctc cggcatcttt
    16801 aacgtggtcg aatcccctca ataataatcc gctgcacgtt ttcaaccgtt tgattgaaaa
    16861 atacctcatc gcgcaacgtt gcgcctgtca ccgcctccac ctgaggggcg aaatcggcgt
    16921 aatgttgagt ggaagcccaa ataataaaaa tcaaatgctg cggatcaatc ggcgcgagtt
    16981 tgccgctttt gacccaaccg gcaatcagcg ccgatttctc atcaattaat gcctt
//
LOCUS       DAJVTY010000084        16998 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVTY010000084 DAJVTY010000000
VERSION     DAJVTY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.084"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OQQ66_002445"
     CDS             complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OQQ66_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS1905343.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            677..1096
                     /locus_tag="OQQ66_002446"
     CDS             677..1096
                     /locus_tag="OQQ66_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1905344.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1096..2363
                     /gene="umuC"
                     /locus_tag="OQQ66_002447"
                     /pseudo
     CDS             1096..2363
                     /gene="umuC"
                     /locus_tag="OQQ66_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OQQ66_002448"
     CDS             complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OQQ66_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1905345.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OQQ66_002449"
     CDS             complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OQQ66_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1905346.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4848..5567
                     /gene="fadR"
                     /locus_tag="OQQ66_002450"
     CDS             4848..5567
                     /gene="fadR"
                     /locus_tag="OQQ66_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1905347.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5619..7151)
                     /locus_tag="OQQ66_002451"
                     /pseudo
     CDS             complement(5619..7151)
                     /locus_tag="OQQ66_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            7481..8779
                     /gene="dadA"
                     /locus_tag="OQQ66_002452"
     CDS             7481..8779
                     /gene="dadA"
                     /locus_tag="OQQ66_002452"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1905348.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFDSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8789..9848
                     /gene="dadX"
                     /locus_tag="OQQ66_002453"
                     /pseudo
     CDS             8789..9848
                     /gene="dadX"
                     /locus_tag="OQQ66_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10235..11971)
                     /locus_tag="OQQ66_002454"
     CDS             complement(10235..11971)
                     /locus_tag="OQQ66_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1905349.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OQQ66_002455"
     CDS             complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OQQ66_002455"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1905350.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13080..13691
                     /gene="emtA"
                     /locus_tag="OQQ66_002456"
     CDS             13080..13691
                     /gene="emtA"
                     /locus_tag="OQQ66_002456"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1905351.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13693..14427)
                     /locus_tag="OQQ66_002457"
                     /pseudo
     CDS             complement(13693..14427)
                     /locus_tag="OQQ66_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            14628..14882
                     /locus_tag="OQQ66_002458"
     CDS             14628..14882
                     /locus_tag="OQQ66_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1905352.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15061..15501
                     /locus_tag="OQQ66_002459"
     CDS             15061..15501
                     /locus_tag="OQQ66_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905353.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15580..16998)
                     /gene="treA"
                     /locus_tag="OQQ66_002460"
                     /pseudo
     CDS             complement(15580..16998)
                     /gene="treA"
                     /locus_tag="OQQ66_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
BASE COUNT         4279 a         4197 c         4396 g         4126 t
ORIGIN      
        1 caaatagcaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat tcatacactg
       61 tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta atatcgccga
      121 ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct tttatggttt
      181 catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga tctaatgctc
      241 catctgcggt ttcgattgcg tttttaacta cttctaccat tttatctgca acgatttcag
      301 tcataatcat tcgcctcttt aaatatataa attgtaatga aactcctgtt ttacaactat
      361 taataaattt tacttcatct aattcatagt tagccgggtg ggatgcgtca atgtctttat
      421 ttctattaat atgataaata tcaaacaatg tttaatgtca ttatggcgaa tgcttctatt
      481 ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc gccagaaggc
      541 cacgtgagca caggataaga gaacgaaaaa tcagcagtct atacagcgac aaatattgat
      601 agcctgaatc agtattgatc tgctggcaag aatagactac tgtatataaa aacagtataa
      661 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      721 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgtcga
      781 acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt acttcgtcaa
      841 agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac tgattgtcga
      901 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
      961 ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca gtgcgtactc
     1021 gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
     1081 taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc cagctgtgag
     1141 acggtgtttc gccctgattt atggggtaaa ccggtggttg tactatcgaa taatgacggt
     1201 tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg cgatccctgg
     1261 ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag caattatgag
     1321 ctttacgcag acatgagcaa tcgggttatg tcgacgctgg aagaactatc gccccgcgtc
     1381 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat
     1441 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1501 ggtgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1561 tggcagcggc agacgggggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa
     1621 tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg
     1681 acgcgatggg gatcaaaacc gttcttgatt tggcggatac agatatccgg tttatccgta
     1741 aacattttaa tgtcgtgctc gaaagaacgg tgcgtgaact gcgcggtgaa ccctgtttgc
     1801 aactggaaga atttgcaccg acaaagcagg aaattatctg ttcccgctcg tttggtgaac
     1861 gtatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg
     1921 aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac
     1981 catttgcgct caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca
     2041 ctcaggacag cagggatatc attaacgccg ctacgcgatc tctggatgcc atctggcaag
     2101 cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg
     2161 cgcagctcaa tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatgacgg
     2221 taatggatac actgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga
     2281 tccaacaaca atggcagatg aagcgagcca tgctttcacc acgttataca acgcgaagtt
     2341 ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagtgctccc gcaggagcgc
     2401 cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg ggaaatcatc
     2461 accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc
     2521 agacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc
     2581 acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaac
     2641 ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca
     2701 ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca
     2761 atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa
     2821 ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta
     2881 aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata
     2941 atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac
     3001 caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc
     3061 atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca
     3121 ttcggttaca ggggcaagcg taaactcgac gcagagcagc ccgacgagtg tcaggacgag
     3181 ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag
     3241 tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cagagggcag
     3301 attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc
     3361 actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga
     3421 aatggatgac agcagaccgt tgaaaatata gaacaacgac agctgagcat gttccgatgc
     3481 ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca ccgcgactac
     3541 cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat
     3601 agcatgttca tcggtgacac cggtcaatga tgtcgccaga ataatgaccg acaaaccaat
     3661 caagccaacc tccgccagat gcaacgccag cgcagtcacc agccagacgc cgataatcgc
     3721 ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa
     3781 ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag
     3841 cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga
     3901 catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg
     3961 tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg catgcatcat
     4021 caggctacgc agaaagccac ggaactgttc cagaaccact ttgtaatgct tgtcgatatg
     4081 actatcgtct tgcaggtcgg tatcttcagt acgggaagag gctacgcggt gataaatacc
     4141 ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc
     4201 ggagaggaac gccgccgcca cgcaaaaaga gagcgacagc agcattttgg aacgaatgct
     4261 taacagcaaa cgggtaaata tgaacagcaa tagctgtttc ataaaataga tacccgccac
     4321 cataaacatc agtaacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc
     4381 cgcgctggtc atgccgatga ataccgcttc aatagccaac agaccaccgg ggagtagcgg
     4441 gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca accagccagc
     4501 gacgaaaggg ctgatgagga aaattaacgg atttacgatt aagaaaatga tgagggcgag
     4561 tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat
     4621 ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa
     4681 agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga
     4741 aaatgaagcc ttgatccctt ttttcttctt tttgtctgct atcagcgtag ttagccctct
     4801 ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg
     4861 cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct
     4921 tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc
     4981 gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac
     5041 atggcaagcc gacgaaggtg aataatttct gggaaacctc tggtttaaat atccttgaaa
     5101 cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc
     5161 gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc
     5221 aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg
     5281 attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgatcc
     5341 ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc aatccggaag
     5401 cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc
     5461 acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc
     5521 ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta
     5581 aaaagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc
     5641 gggcaacgtt ccagcaactc gacgctgcca tcttcgtttt gctgttccag catcacatca
     5701 aatccccaca ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg
     5761 cgattatgtg gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag
     5821 atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc
     5881 tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcgtca
     5941 tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa ctggctgata
     6001 aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg
     6061 gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata
     6121 tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca cgggctgtta
     6181 taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc
     6241 gttactttcc cttcatcata cagatggtta aggatggtgt agtgccagaa ggtggcccag
     6301 ccttcgttca tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca
     6361 atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag
     6421 agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc
     6481 tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata ctcttcgcga
     6541 cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc
     6601 acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc atccacgcca
     6661 taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc gacaatcgaa
     6721 ctggcgtcgg ttcaactgcg gaataagtaa ttgtttttaa agaaagagtt atgcccatag
     6781 caggcgtgtg ccatcaccag cgcttgcatg gtaatggtgt tctcttccat caggtaagcg
     6841 atacacgggt tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac
     6901 agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg
     6961 ccgacgctgg agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg
     7021 taggtatcca gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc
     7081 aggtcgaacg tccagtcggg tccatcgctc aaccgtgtgg tgtccttatt catagaatcg
     7141 atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc
     7201 aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt gcgatttctt
     7261 attatcagag tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt
     7321 tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc
     7381 accataaaaa agctgtatgt tgaataatat tttcaactga gttatcaaga tgtaattaga
     7441 ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg
     7501 aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac
     7561 cgtcattgat cgggagccgg gggccgccct ggaaaccagc gccgcgaatg ccgggcaaat
     7621 ctcccccgga tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg
     7681 gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa
     7741 atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg
     7801 gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa
     7861 tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta
     7921 tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct
     7981 ggaatccagc cgcctggcgg aagtggagcc agcgctggca gaagtagcgc acaaactgac
     8041 gggtggtctg caactgccca atgatgaaac cggagactgc cagctgttta cccagaatct
     8101 ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact
     8161 gctttgcgac ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga
     8221 tgcgtatgtg atggcgtttg attcttactc gacggcgatg ctcaaaggca ttgttgatat
     8281 tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc
     8341 gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg
     8401 cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg
     8461 tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca
     8521 ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg
     8581 tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc
     8641 ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata
     8701 tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt
     8761 acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca gcctcgatct
     8821 gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgggtggtaa
     8881 aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg gccaccgatg
     8941 gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc tggaaggggc
     9001 cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc
     9061 gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa
     9121 aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc
     9181 ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt ggcgagatga
     9241 ccctgatgtc gcattttgcc gaagcggaac atcctgatgg aatttccagc gcgatggcgc
     9301 gtattgagca ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga
     9361 ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg
     9421 cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc
     9481 taagcagtga gattattggt gtccagacgc taaaagcggg tgagcgtgtg ggctacggcg
     9541 gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcaggg tacgccgacg
     9601 gttatccgcg ccacgcgcct accggtaccc ctgttttagt ggacggcgtg cgcaccatga
     9661 cggtggggac cgtctcgatg gatatgctgg cggtcgattt aacgccttgc ccgcaggcgg
     9721 gtattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg
     9781 ctgccggaac ggtgggctat gagttgatgt gcgcgctggc gctacgcgtc ccggttgtga
     9841 cggtgtaact tgttgtaagc cggatcggag gcaacgtctt ctgggtgcaa aaaaaatcat
     9901 ccatccggct ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg
     9961 tccattaaaa ggatggacac tatttccccg gaacctgaac tcaccgcaca ggcgttctac
    10021 ataaaacgct tacgcttcat tgttgactcg gagtcgactt cagataaatc acgctttacc
    10081 cttgatggag cctgtacata gatttgtgta attgcctgat tttgatatgt tcaattcaac
    10141 atcaaatgaa ggttaaatta tggacgacaa acaattgcag gctcaggctg cgttcagcaa
    10201 agcatcgcaa ccggcgatag atgcttcatt aaatttaaga ttcagcttcc tcttcagcca
    10261 cccgtacgcc aatcttcaac acttcattat cttctttctc ggccaccgtc cagatcatcc
    10321 cggcaaactc tacctggtca ccgacaaccg gtgccgtgcc taacaactgc tggacaattt
    10381 cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg
    10441 caacatcagc atatttggcg ctggcttcga gaatgaagtc accaaagaag cgttgatcca
    10501 gcgcgaccgg cggcgactgg ctgaacagtt taccgagcgc cggaagatcg cgttcccgac
    10561 caattacaca caacacatcg ccttcgcgca gtcgggtgct gccggtagga tgaagcaact
    10621 ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg
    10681 ccgcgcccac gcaccattta tcggcactca attgataaac aaactgctcc cacggatttt
    10741 ccggatgaat atccaggcca acgcgtgaca ccggacgtcc cactggcgga accaccactt
    10801 tggctttttt agccgcccac gagagtgatg ttccctgcaa gagcagtgaa accagaacca
    10861 caaagaaggc gacattaaag aacagacgtg cattctccag ccccgccatc atcgggaaca
    10921 cggccaggat gattggcacc gcgccgcgta atcctaccca gctgataaac acgcgctcac
    10981 gcagattgaa accgcggaag gggagcaatc cagcaaatac cgaaagggga cgggcgaaga
    11041 atatcatcca tgccgacaaa attagcgccg gaatggcaat aggcagcaga tcgcttgggt
    11101 taaccaatag ccccagcacc aggaacatgg cgatttgcgc cagccaggcg aggccgtcga
    11161 aattttgcag gatgccgtag cggttgcgaa tcggccgatt acccagcaga aaaccgcaca
    11221 gataaacagc cagaataccg ctgccttcca gcgccgtggt taaagcaaaa attaaaatac
    11281 cgccgcttaa tgccagcaat ggatataatc cggcaggcag ggcgatgcga ttaatcattt
    11341 gcagcagtaa ataaccaccg ccaagtccga tgacaatccc gaggccaaat tgctgcagaa
    11401 tatcgaccac gaacatccaa ctgacactgc tttcatgttg ctggatcatc gcaatcaggg
    11461 taatcgtcag aaagaccgcc attggatcat tactgccgga ttcaatttcc agcgtcgagc
    11521 caacacgttc gttaagcccc ttaccaccca gcagagaaaa gaccgctgca gcatcggtag
    11581 agccgacgat agcgccgatt aataagcctt caatcaaatc aagattaaac agccacgccg
    11641 ccatcatgcc ggttaaacca gaggtgataa gcacgcccag cgtcgccagc gacagtgccg
    11701 gtcctaacgc cacacgaaag gaactggcct gagtgcgcat cccgccgtcg agcaaaataa
    11761 tcgccagtgc caggttactt accatgtagg cgaaggggta attatcaaac gggataccgc
    11821 cgacgccatc gactcctgcc agcatgccga tcgccagaaa aataaccaga ataggaatgc
    11881 caagacggga agaaaatgaa ctaagtaata tactgctggt gacaaggatg gatcctaaga
    11941 tgaaaaggct aattattgtt gtggcatcca acggtcggtt actcctgatt acgctgtctc
    12001 ttatataaac cctaccatat tagcggcaga gacagcgttt tacttagccc tgaagcgtga
    12061 tttttttaca ttttaagaac aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg
    12121 ttattcagaa tggcatgtgc gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa
    12181 tcgaggccgg taatgagtgg aatcgacagg cgggaacgca aaaacgcgga cactgactcc
    12241 aggttgtaac ctgcgtcata atcattgggc gtgctgccgc taaagctacc gagaataatc
    12301 gccttctgac gtggcaaaat tcccgcatga tagagctgca acagcatacg ttcgacgcgg
    12361 aaagggtgct cgttaatatc ttccagtacc aggataccgt tctcaatttt tggcatccac
    12421 ggtgtaccaa tcagtgaaat cagcatcgca agattgcctc cccacaacgt gccttcggtc
    12481 tgacatgtcg ggccttcccc ttgccattca atggtgaagg ttttattgcg taacgccagc
    12541 cagaaatggt gctcggtaaa ggcgttcagt tcatccgcac caaagtttgc taccagcatg
    12601 gggccgctaa aggtgatgac attgccttgc gccagaagac cgcactgaat ggcggtaaaa
    12661 tcgctatgtc cgcaaatgag taacgggtca tgttgttggc gagccaccag tgcctgccag
    12721 tcaatatctg ccagtaaacg actggcaccg taaccgccgc gtacagccag cacgatggtg
    12781 ttgggtgtag tcagtctggc aagggaattg agatcttcca gacgctccgt ttccgtaccc
    12841 gcaaaacgct cacaacgacg ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg
    12901 gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca
    12961 attaagtgaa acagagacat ggcaattcct tgctgacaac agaaacgaaa tgtatatcat
    13021 gccgcttagg tgtgccgttg tcacctcaac ggcgattcca ggctataagg atagaagaag
    13081 tgaaattgag atggtttgcc tttttgattg tgttattagc gggttgttca tcaaagcatg
    13141 actatacgaa cccgccgtgg aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc
    13201 caataagcca gaaagccggt gcagcctggg gcgtcgatcc acaattgatc acggcgatta
    13261 tcgctatcga atcgggtggt aatccgaacg cggtgagtaa atcgaatgcc attggtttga
    13321 tgcagttaaa agcttcaacc tccggacgtg atgtttatcg ccgtatgggc tggagtggtg
    13381 agccgacgac cagcgagctg aaaaatccag agcgtaatat ttcaatgggg gcggcttacc
    13441 tgaatattct ggaaaccggc ccgctggcag gcattgaaga tccgaaggta ctgcaatatg
    13501 cgctggtggt gtcatacgct aacggggcag gtgcgctgct acggactttc tcgtcagatc
    13561 ggaaaaaggc gatcagcaaa atcaacgatt tggatgctga cgagttcctc gatcacgtag
    13621 cgcgcaatca tcctgcgccg caggctccgc gctatatcta caaacttgag caggcactgg
    13681 acgcgatgta aatcagtcgc gtactttgtc cgctttctcc cgggcttccc gctcgagaga
    13741 gaaaataatc cgttgtaatt gccgctccac cgtcgggctg acgttaagaa aacggaagct
    13801 cagacgggga gtggtgatgg tttcattctt gccatcaatc actttgcgct cgctgatgga
    13861 gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc atgttgactt caatctgagc
    13921 gaagcgcatg ccttcttgta attcggcagg ctttgctgtt tccagtaatg cgcccatgcc
    13981 gcctaacgac aaatcataca ggcggaaacg taacgtactg ttatccgcca gtttggtctg
    14041 gcaaaaataa ggcggatgga gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa
    14101 ccataaggtg ggaggcggta cggtaataaa tgccggaagc tgcaagtatt cactctactg
    14161 tagttgttca acagtaaact cgactttcgc accctgagtt tcggcggtaa tggtaatatg
    14221 ctgcgccttt agcacggcga tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt
    14281 atccggggtt attgccagta ttttgctgat cagctgcccg ccattccaac tgagacgcaa
    14341 aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg acggctaacg gattttgttt
    14401 caggaactgc tcatggtaat gactcacgcg gaaaaactcc tgactgacaa actgtctttg
    14461 gtttatcggt cacagttaac aaaacttaat acaaatgcgt gaatattttt tacatgttgt
    14521 tcttaaatca gccgacatac gcccagcatc gatcccgccc ttgcctatac ttagagcgtt
    14581 gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg cttgaacatg ggaattattg
    14641 cctggattat ttttggcctg atagccggca ttatcgccaa gctaatcatg ccggggcgtg
    14701 atggtggtgg atttttccag acctgtattc tcgggatagt cggtgcggtg gtcggcggct
    14761 ggctggcgac catgtttggc attggcggct ccatcagcgg ttttaatttg cacagcttcc
    14821 tggtggcggt ggtgggagct attctcgttc tgggcttatt ccgcctgctg cgaagagaat
    14881 aagattttca taaggcggat agcgatacag atgccgctat ccgcttttca catcagaacg
    14941 tatattcgac acctgcttta cgggtgaaaa aaatcaatat agcacttcga tatccgataa
    15001 agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt tgtttttggt
    15061 gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa tggggcggat
    15121 agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc ttctggcaga
    15181 ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg gtctgaattc
    15241 tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa tttaactcca
    15301 gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc aatcgatgct
    15361 ttatataggc aacgtcggtg taaatcaccg ggggatgata gtgaaacact tacccgtaga
    15421 aatgacgcta tcttcggcaa ccacgtatgg caaacatttg cgcaatactt cccgccagga
    15481 cttgaaaaac cggctgtgta gaaaacacca tagctatggt gttatctgcc gacaacgcca
    15541 tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg gttgtgcctc
    15601 tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg gaacattgtc
    15661 acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc cattggtcca
    15721 gccaaagcca tcctgtaatg gatattcgcc acctcttttt gcccgtagtt ttgtaatcct
    15781 tctgtcgcga cccactgtaa cggtgcccag ccatttggcg catcccattg ttgcccactt
    15841 ttcaccgacg tggtgttcag gccgccgggt tgcagcagat gtgttttcgt cgccgtcgcc
    15901 attttgttgg cgcgatcttt cgctgccgca ttgacgtaca gcgggaacag ggcggccgcg
    15961 gttaactgat tgcgcacttt atgacttttc aggtcgtaat cggcatacca gccttgttga
    16021 tcgttccaca ggtatttttc gattcctttt tgacgggcat tcgccagcgt ttcgtactgg
    16081 tttgccatcg cgttatctcc tgcagctttg ctggcgcggg cgaggatttt ttccatttta
    16141 aacatcaggc tgttcagatc gaccggtacg atgctggtgg tgcgtaaggt atttaactgc
    16201 tgcgggttgt ccatccagcg cgagctgaaa tcccagccag acgcagcggc agagcgcagg
    16261 tcgcggtaaa tttcagtggc aggtcgattc ggattgcttt tggcggtggc aatatcttcc
    16321 acccatgact ctggtcgtgg cgtatcgcga tcgtcccagt agcggttgag aagggtacca
    16381 tcctgaagtt tgacaacgcg tttttcctgt tgtccggctt gcaggttttc aacaccgtcc
    16441 atccagtaag catattcttt ttgcatttgc ggcaggtatt gcttcaacgc ggcatcgcct
    16501 tcatgctgcg ccagtaactc taccatcagg gcaaagaagg gcggttgcga gcggcttaaa
    16561 tagtaactgc ggttgccgtt gggaatatga ccgtaagtgt ctatttcatg agcaaaattg
    16621 gccaccatat ccgcgacttt atcccagtga ccgctttcgg caagtcctaa catggtgaag
    16681 taactgtccc agtaatatac ctcgcgaaaa cgtccgcccg gcacgacata aggttccggc
    16741 agcggtaaca gagaatccca tttttcggtg ttttcggtag aacgcgttaa taccggccaa
    16801 agtccgtcaa tatgttcgcg cagtgactgc ccctctggcg gaacatattt ctcgccttct
    16861 ttcggcaggg tgaaattgac gttaacgaaa tggcgcagat caaatccgct ctggttttgc
    16921 tgcatccgat aatcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg
    16981 gaaaaagttt ggcgtttt
//
LOCUS       DAJVTY010000085        16953 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVTY010000085 DAJVTY010000000
VERSION     DAJVTY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16953
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.085"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..2099)
                     /gene="topA"
                     /locus_tag="OQQ66_002461"
     CDS             complement(<1..2099)
                     /gene="topA"
                     /locus_tag="OQQ66_002461"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1905354.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFR"
     gene            2479..2730
                     /locus_tag="OQQ66_002462"
     CDS             2479..2730
                     /locus_tag="OQQ66_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1905355.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(2766..3815)
                     /gene="sohB"
                     /locus_tag="OQQ66_002463"
     CDS             complement(2766..3815)
                     /gene="sohB"
                     /locus_tag="OQQ66_002463"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1905356.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4035..4793
                     /locus_tag="OQQ66_002464"
     CDS             4035..4793
                     /locus_tag="OQQ66_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1905357.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            4790..5381
                     /gene="cobO"
                     /locus_tag="OQQ66_002465"
                     /pseudo
     CDS             4790..5381
                     /gene="cobO"
                     /locus_tag="OQQ66_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5421..6293)
                     /gene="rluB"
                     /locus_tag="OQQ66_002466"
     CDS             complement(5421..6293)
                     /gene="rluB"
                     /locus_tag="OQQ66_002466"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1905358.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(6504..8399)
                     /locus_tag="OQQ66_002467"
     CDS             complement(6504..8399)
                     /locus_tag="OQQ66_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCS1905359.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8427..9047)
                     /locus_tag="OQQ66_002468"
     CDS             complement(8427..9047)
                     /locus_tag="OQQ66_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1905360.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9044..9925)
                     /gene="yciV"
                     /locus_tag="OQQ66_002469"
     CDS             complement(9044..9925)
                     /gene="yciV"
                     /locus_tag="OQQ66_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1905361.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10063..10107
                     /gene="trpL"
                     /locus_tag="OQQ66_002470"
     CDS             10063..10107
                     /gene="trpL"
                     /locus_tag="OQQ66_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1905362.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10084..10177
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10199..11761
                     /gene="trpE"
                     /locus_tag="OQQ66_002471"
     CDS             10199..11761
                     /gene="trpE"
                     /locus_tag="OQQ66_002471"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1905363.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            11761..13356
                     /gene="trpD"
                     /locus_tag="OQQ66_002472"
     CDS             11761..13356
                     /gene="trpD"
                     /locus_tag="OQQ66_002472"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1905364.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGICFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13357..14718
                     /gene="trpCF"
                     /locus_tag="OQQ66_002473"
     CDS             13357..14718
                     /gene="trpCF"
                     /locus_tag="OQQ66_002473"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1905365.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            14730..15923
                     /gene="trpB"
                     /locus_tag="OQQ66_002474"
     CDS             14730..15923
                     /gene="trpB"
                     /locus_tag="OQQ66_002474"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1905366.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            15923..16729
                     /gene="trpA"
                     /locus_tag="OQQ66_002475"
     CDS             15923..16729
                     /gene="trpA"
                     /locus_tag="OQQ66_002475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1905367.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4098 a         4530 c         4230 g         4095 t
ORIGIN      
        1 cggaattcgc cctcttcgat ctcgtaaccg tcgcaggttg ggttattacc acagacatgc
       61 aacttacgtt tcggatcgat gagatagctg tccatcgccg tgccgcattt cgggcaacga
      121 cgttttgcgc gcagcgcgtt ggtttcagcg tcttcgcctt ccagtacgtt cagcacttcg
      181 ttttccggca ccaggttaat ggtggttttg caacgctctt tcggcggcag cgcataacca
      241 gaacagccaa ggaatacccc ggtgctcgct gtgcgaatcc ccattttgcg accacaagtc
      301 gggcagtcaa tgctggtcag aaccatctgg ttcgggcgca taccaccctc ttccggatct
      361 ttttcagctt tatctaactg ctgggtgaaa tccgagaaga agtgatccag tacagctttc
      421 cactctgctt cgtgatttgc cacctggtcg aggctgtttt ccatctgtgc ggtgaagtcg
      481 tagttcatta actcgcggaa attttcttca aggcgatcgg tgacgatttc gcccattttt
      541 tccgcataga aacgacgatt ttctactcgc acgtagccac gatcctgaat ggtcgaaatg
      601 atcgacgcat aggtagacgg acgaccgata ccgcgttttt ccagctcttt aaccagcgat
      661 gcttcactga aacgggctgg cggcttggta aagtgctggg ctggtgtaag ttcaacgagc
      721 gtcagagcat cgcctttatt aactgctggt aagatgcgat cttcatcgcc tttacgcaac
      781 gcaggcatca cttttgtcca gccatcaaaa cgcaaaatac gaccgcgtgc tttcaggcgg
      841 aaatcgcccg caccaacggt cagcgtcgtg gagtcatatt tcgctggggt catctggcag
      901 gctacgaact gacgccagat taactggtac agtttctgcg catctgcttc catatccttc
      961 agcgattccg ccatcacatt gacgtcagaa ggacgaatcg cttcgtgcgc ttcctgtgag
     1021 ttttctttgc tggcgtactg attcggactt tccggcagat atttcttacc aaaattatcg
     1081 ctgatataac cgcgaaccat atttaccgcg tcctgactca ggttagtgga gtcggtacgc
     1141 atgtaagtga tatagcctgc ttcatacaaa cgctgcgcca tcatcatggt ttttttcacg
     1201 ccaaatccaa gacgggtgct ggcagcttgt tgcagcgtag aggtaataaa aggagcgcca
     1261 ggtttactgg ttgtcggttt gtcttcacgt tccagcacgc tgtagcgcgc tttttccagc
     1321 agacttaccg cagcctgagt ttgttctttg ttgaccggac ggaacggttt gtcgttctga
     1381 tgagtcacct gtaacgccaa cgcttcacca gatggcgtgg tcgtgctggc atcgacttcc
     1441 cagaactctt ccggcacgaa cgctttaatt tcacgctcac gctcgaccac caggcgaacc
     1501 gccaccgact gcacacgacc ggcagacaga ccacgagcga tctttttcca tagcagcggc
     1561 gaaaccatat accccaccac gcggtccata aagcgacgcg cctgctgggc attaacacga
     1621 tcaatattca gctcacccgg tttgttaaat gcctggcgga tcgcgttttt agtaatttcg
     1681 ttaaacacca ctcggctata gcgcgcatca tcacccccaa tcacttcccg caggtgccat
     1741 gcaatggctt ccccttcgcg gtcaaggtcg gttgcgagat agatgtggtc ggctttttca
     1801 gccagttgtt tcagttcaga gacgaccttc tctttaccag gcaacacttc atagtgcgcc
     1861 tcccaattgt gccacgggtc aacccccata cggttgacga gagcgccacg ttcatccttt
     1921 ttaggctttt tagccgtctt ggtggaggta gagtcggcac tctttttggc agctgagcca
     1981 ctggtcggca aatcgcggat gtgaccgacg ctggatttca ccacgtagtc actacccaga
     2041 tacttgttga tcgttttggc ttttgccggg gactcaacga tgacaagagc tttacccata
     2101 ttcaccttac ctaatttgat tcttccagga atgcgtcgca cgttgataaa ccttccactg
     2161 gcaacgagtt accgatatgg actcgttctc tgcggatatc aaccccttgt cgtgactctg
     2221 ttcacgaaaa ttcaggtgac tgagttaaca ggtcttggcc tacaggcgct atagcacgac
     2281 ggaattttgg tcgaatgtca agcaaatcta ttgccagatt gacgaaagcg tcacactgta
     2341 cctgataaaa tccgatacgc aactttatta gcatgcaaaa tccaaagttg ccagtaaggc
     2401 gctcttgcca ccagcgtggt atatttgtct gcaagccatg cctggcgtag actaatgtcc
     2461 ttatttaagg agtaaataat gaacaaagag acacaaccca tcgaccgcga aacgctgttg
     2521 aaagaggcca acaaaatcat tcgtgagcat gaggatacgc tcgcagggat tgaagccacc
     2581 ggcgtcacgc agcgtaatgg cgtgctggta tttaccggcg actacttttt agatgaacaa
     2641 ggattgccaa cagccaaaag caccgcggtg tttaatatgt tcaaacacct ggcccatgtg
     2701 ctttcagaaa aatatcatct ggttgattaa agcaaaacgc gaggtcttag cctcgcgttt
     2761 gtcttttaca tcaatggctt ttgaccccgc tgccaccagc gtagcaacaa tcgatcggcg
     2821 ctctctgccg cgctgccggt gaatcggtca atgagtcgtt tacgctgcat atagcgtaca
     2881 ttaaccactt cacggccttc catcaggcta agaataactt catcactggt gttgatttca
     2941 tcaaccaggc ctttctctac cgcctgttgt ccgtaccagt gttcacctgt tgccacctgt
     3001 tcaatatcca gagacggacg catacgcttc acaaaatctt taaacaactg atgcgtttcg
     3061 ttcaactctt cgcggaattt ctcccgccct tcttcggtat tttcacccag caacgtcaga
     3121 gtacgcttat actgcccggc agtgtgcagt tcgatatcaa tatatttgct tttcaggaag
     3181 cggttaaagt tgggcatttg cgccaccacc ccaatggaac ccacaatagc aaacggtgcg
     3241 gaaacaattt tgtccgccac acaggccatc atgtaaccgc cgctggcagc gactttgtct
     3301 accgtaacag ttaaaggaat gtttttatca cgcagacgct gcagctgcga agccgccaac
     3361 ccgtaaccat gcaccatgcc accagggctt tccagacgga gcacaacctg atcctgcggt
     3421 ttgaatgctg cgagtaccgc cgttatctct tcacgtagcg agttcacttc atgggcgtcc
     3481 atgctgcctt taaaatccag cacccagacg cgaggtttac tgtcagttgc cacctcgccc
     3541 agtttggctt tcgcttttgc tgctttcgct tcttgcttgt gctttttctt ctgcgctttg
     3601 tgccactgtt tttgctgatg tgagcccatc agcgccgcgg ccagttcttc tttcatctcc
     3661 ttatactgtt cgctgagatt gttgacccgt aactcgccac gctggcgttt attacgttga
     3721 gcaacattga caataatggc ggcaatcgcc gcaatcgcta gcacaacggt aacgattttc
     3781 gccaaaaaca aaccatattc agacaacaat tccacgagtc cccaccttgg ttaaacaacg
     3841 ctttggctca cagtttacaa caggcatgtt aagccgtctc gcagagatgt tattgcagcg
     3901 aaagtatgac aaagtatcca ttttgcggtg ttatcgcccc aggctgattg aaatgccctt
     3961 ctgtttcagg cataaagccc caaagtcata aagtacactg gcagcgcgac agacaacgtc
     4021 gttgaggagt agccatgcat taccagccaa aacaagattt actcaatgat cgcattatcc
     4081 tggtgacggg agccagcgat gggattggtc gtgaagccgc gatgacgtat gcacgctatg
     4141 gtgcgacagt gattctgttg ggccgtaatg aagaaaaatt acgtcaggta gccagccaca
     4201 taaacgaaga aactgggcgt cagccacagt ggtttattct cgatttgctg acctgcacgt
     4261 ccgaaaattg ccaacaactg gcacagcgca ttgtcgttaa ttatccgcgt ctggatggtg
     4321 ttttgcataa tgccggattg ctcggcgata tttgcccaat gagcgaacaa aatccgcagg
     4381 tctggcagga cgtcatgcag gtcaacgtta atgccacctt tatgctcacc caggcactgc
     4441 ttcctttatt actcaaatcg gacgccggtt cactggtctt tacttcatca agcgttggac
     4501 gtcagggacg agctaactgg ggtgcatatg cagcgtcgaa atttgccacc gaagggatga
     4561 tgcaggtact ggccgatgaa tatcagcagc gcctgcgcgt caactgcatt aacccaggcg
     4621 gtacgcgcac cgcaatgcgc gcaagcgcct tcccgaccga agatccacag aaacttaaaa
     4681 cacccgctga tatcatgccg ctctacctct ggctgatggg cgatgacagc cgccgtaaaa
     4741 ccggcatgac ctttgacgcc caaccgggcc gtaaaccagg aatttcccaa tgagtgatga
     4801 acgctaccaa cagcgtcagc agcgagtgaa agaaaaagta gatgctcgtg tggcacaggc
     4861 cccaggatga acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg
     4921 cggcatttgg tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt
     4981 ttattaaagg cacctggcct aatggcgaac gcaatctgct ggaaccacat ggcgttgagt
     5041 ttcaggtgat ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg
     5101 cctgccgcga agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg
     5161 ttttgcttga tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg
     5221 tgcaggcgtt aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc
     5281 atcgggatat tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt
     5341 ttgatgccgg tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata
     5401 tgacgtccgg catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc
     5461 acctgactgt gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct
     5521 ttttctacag cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag
     5581 ttagtctggg cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg
     5641 atatcaccgt aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga
     5701 cgacgaactt cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct
     5761 tcgccgccgc tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca
     5821 cgactcaaat cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca
     5881 cgttcaactt cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac
     5941 agcagcagac cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc
     6001 agctttggca gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac
     6061 tcaccttccg gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc
     6121 gaaatcagat gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg
     6181 agtttagcaa ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca
     6241 cggcgggagc cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt
     6301 ccttttggtg tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact
     6361 tacatctgac tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact
     6421 cgacgtttat cggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct
     6481 tgtttcgatt aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgccg
     6541 ccagcaccac caccggaata acttcgcccc aacaaactgg tattggcccc gcaacttagg
     6601 tgcgtctgta atgagaataa aacgccgtta cgggcaatct ctggagcgcg aatcgccgaa
     6661 gaaagcgaag gggcaatgcg taacgcccat tccctcccca aatttgccga tatagcccaa
     6721 cctagcaacg cgtaatccag tttccgtgtt tcccctttcc gccgacgtcc actgtgtgtt
     6781 gctgcctctt tacgcgcaag atagttaacg atagcttcac cgcgggcata acgttgctgc
     6841 ccagtttggg taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg
     6901 caaacagcgc taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg
     6961 atatagagag aatagaaggc cacgcctgaa caaatcagtg gtaaaaacaa agtgatgatt
     7021 gcacgattaa cgcaaggctt gtcatcatcg cgggcaaaca aaagctcaag ggaacaaaat
     7081 aaaaaaggaa taatccagca gatcataata aacatatcgc ctaccagact cattcctgcc
     7141 caacctacgg gactcaggtt accgtagaga atgatcgcca atattgccag tccaccccat
     7201 tgcaaaaacg tatcaggccg gtaaaacaaa gactttttac gcggcagata aaatttctgc
     7261 atccgtaaaa atgccctgcg taagactctg ttgcgtctcc ccgtaagaac gaccttatcg
     7321 cctttacgaa ataacgcttc cagtaataac tgctctgcgg gcagtaatac ctcattattt
     7381 ctggtcgcgc cttcattaac acaaatcagg atttgctggt tcttatcata ctgatcttcc
     7441 agatgaattt ttcccttcac gatcagattt acgatatcgg cacagaaacc tttatcgtcg
     7501 taaaccagct tcgcatcgag acgtaacatg ccgggcgtgt aatctggcgg aatgacatcg
     7561 gtttcaatca catctaccgg cgtaaattgc ggcctgcgct tccagagata taaccatcca
     7621 caaaccagga atagacacgg aaaccaaatt aacaacgatg acgtggaggg taaaagaaga
     7681 tggctgaaaa tattcgtcgt ttgtggagcc ggggcattgc taagtaacgc gtgaggccag
     7741 cggtagagta ccgtgaaatc ttcacgataa aacggatcgc gggattcaat tcttccgtcc
     7801 tcaaggatgc ggccatttcg atatgtgtcg ccctcttctc cagtaaagag atcgatttcg
     7861 ctaaatggat tacccgcgat atcagggaac ttgagtgaaa atcgggtctt atagatttca
     7921 aacggccagt ggttaccagt cacgttccag attaacagat ctgaatctcc ctcacgcagg
     7981 aaagcatttt taacctgata atgaatttga taatgatagt cgccttgagg caagaaacgt
     8041 tgtttatcgc ccacgacaat actcattaac ccgatatgcc agtcaagatg ccagggttca
     8101 ggaataccat cacgagtgac gccaacaata tgatagttca gcagtgcagc gtccccatca
     8161 ggctgcatcc agagtcgtgg taaatcacga taaaatccgt gcttaatctg ctgatttcgc
     8221 gcctgaaaat gaatattttc tttgatgtcg agcaccccat ccggttgcag agtggcttca
     8281 acatcaaaga gcgagatccc ttcttcataa gaagggatct ccgggttttc tgcataactt
     8341 agagatgata caaacagccc tgctatcaac agcaaaatgc agcgaaactt ccctgccata
     8401 gtcttccatc cgtggaacgc aatttgttat aagaaaggct tcacatcacc cacgccttca
     8461 cgcaccacga ccggcgtatc atcggtaaga tcaatgaccg ttgtcggttt ctgtccgaga
     8521 tagccgccgt ggataatcaa atccacctgt ttttccagac ggtctttaat ttcttctgga
     8581 tcggattcgg taaattcgct gcctggcagc attagcgaag tggaaagcat cggttcgccc
     8641 agtgcttcaa gcaacgcctg cgcaatcggg ttagaaggca cacgcatacc gatggttttg
     8701 cgtttttcct gcaacagacg gcgtggcacc tccttcgtcc ccttcaggat gaaggtatag
     8761 ttgcccggcg tgttgttttt cattaaacga aacgcaacgt tatccacgaa tgaataggtc
     8821 gaaagttcag aaagatcgcg acacatcagg gtaaagttgt gaccgtccgg cagctggcga
     8881 atacgacaaa tacgctccat cgcgttttta tcttcaattt tacagccgag cgcatagccg
     8941 gaatcagttg gataaacaat caccccgcct ttacgcacga tctccaccgc ctggttgatc
     9001 agacgttgct gcgggttatc aggatgaata taaaaaaact ggctcataat tccctctctg
     9061 tggtgttctg cggctgttcc catagctgcc agacgccttc aacgcctgcg ggcagccaga
     9121 gtttacgacc cagttcgatc cacgggcatg gctgatgaaa atcagatcct tgtgatgccc
     9181 ataaatgatg ctgacgcgca agtgtcgtca actgggtgcg ttcattgggc gattgctggc
     9241 actgcgcgac ttccatcgcg tcgccgtggt gttcggcaaa atgcgcaacc aatcttttca
     9301 gccatttagc actaagattg taccgcccag gatgagctag caccgcctta ccgccagaat
     9361 gatgaatgac atcaatagct tgttctattg tacaccactg tggcggaacg tatccggttt
     9421 tcccgcgcgc cagatacttt ttaaagacat ctgccattga actggctttg ccgcactcaa
     9481 caaggaaacg cgcaaaatga ccgcgcgtca ctgcgccgcc ctgcgccagt cgttgtgcgc
     9541 cttccagcgc cccaggtatt tgcgcttttt caagtctttc ggcaatttgc tgcgcccgct
     9601 gattgcgccg ttctgtctgc tgcgcgagga actcacacat tagcggatga gtaatatcaa
     9661 tattcagccc gacaatatga atttcatgat tttcccagac cgtggaaatt tccacgccgg
     9721 gaataagatt cagcgccagt cccgaacgtg aaatttcctc tcttgctggc gcgattgcag
     9781 ctgtggtgtc atggtcggtg atcgccaggg tgccgacgcg catctcgact gcacggtgca
     9841 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa
     9901 tcactgcgta attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc
     9961 gacatcataa cggttctggc aaatattctg aaatcagctg ttgacaatta atcatcgaac
    10021 tagttaacta gtacgcaagt tcacgtaaaa agggtatcga caatgaaagc aattttcgta
    10081 ctgaaagctt ggtggcgcac ttcctgaaac gggcagtgta ttcaccatgc gtaaagcaat
    10141 cagataccca gcccgcctaa tgagcgggct tttttttgaa caaaattaga gaataacaat
    10201 gcaaacacaa aaaccgactc tcgaactgct aacctgcgaa ggcgcttatc gcgacaaccc
    10261 gactgcgctt tttcaccagt tgtgtgggga tcgtccggca acgctgctgc tggaatccgc
    10321 agatatcgac agcaaagatg atttaaaaag cctgctactg gtagacagtg cgctgcgcat
    10381 tacagcatta ggtgacactg tcacaattca ggcactttcc ggcaacggcg aagccctgct
    10441 gacactactg gataacgccc tgcctgcggg tgtggaaaat gaacaatcac caaactgccg
    10501 tgtgctgcgc ttcccccctg tcagtccact gctggatgaa gacgctcgct tatgctccct
    10561 ttcggttttt gacgctttcc gtttattgca gaatctgttg aatgtaccga aggaagaacg
    10621 agaagcaatg ttcttcggcg gcctgttctc ttatgacctt gtggcgggat ttgaagattt
    10681 accgcaactg tcagcggaaa ataactgccc tgatttctgt ttttatctcg ctgaaacgct
    10741 gatggtgatt gaccatcaga aaaaaaacac ccgcattcag gccagcctgt ttgctccgaa
    10801 tgaagaagaa aaacaacgtc tcactgctcg cctgaacgat cttcgccagc agctgaccga
    10861 agccgcgccg ccgctgccgg tggtttccgt gccgcatatg cgttgtgaat gtaaccagag
    10921 cgatgaagag ttcggtggcg tggtgcgttt gttgcaaaaa gcgattcgca ccggagaaat
    10981 tttccaggtg gtgccgtctc gccgtttctc tctgccctgc ccgtcaccgc tggcggccta
    11041 ttacgtgctg aaaaagagta atcccagccc gtacatgttt tttatgcagg ataatgattt
    11101 caccctgttt ggcgcgtcgc cggaaagttc gctcaagtat gacgccacca gccgccagat
    11161 tgagatctac ccgattgccg gaacacgccc gcgcggtcgt cgcgccgatg gttcactgga
    11221 cagagacctc gacagccgca tcgaactgga aatgcgtacc gatcataaag agctttctga
    11281 acatctgatg ctggtggatc tcgcccgtaa tgatctggca cgcatttgca cccccggcag
    11341 ccgctacgtc gccgatctta ccaaagttga ccgttactct tacgtgatgc acctggtctc
    11401 ccgcgtggtc ggtgagctgc gccacgatct cgacgccctg cacgcttacc gcgcctgtat
    11461 gaatatgggg acgttaagcg gtgcgccgaa agtacgcgcc atgcagttaa ttgccgaggc
    11521 ggaaggtcgt cgccgcggca gctacggcgg cgcggtaggt tatttcaccg cgcatggcga
    11581 tctcgacacc tgcattgtga tccgctcggc gctggtggaa aacggtatcg ccaccgtgca
    11641 agccggtgct ggcatagtcc ttgattctgt tccgcagtcg gaagccgacg aaacccgtaa
    11701 taaagcccgc gctgtactgc gcgctattgc caccgcgcat catgcacagg agactttctg
    11761 atggctgaca ttctgctgct cgataatatc gactctttta cgtacaacct ggcagatcag
    11821 ttgcgcagca atgggcataa tgtggtgatt taccgcaacc atattccggc gcagacctta
    11881 attgaacgcc tggcgacgat gagcaatccg gtgctgatgc tttctcctgg ccccggtgtg
    11941 ccgagcgaag ccggttgtat gccggaactc ctcacccgct tgcgtggcaa gctgcccatt
    12001 attggcattt gcctcggaca tcaggcgatt gtcgaagctt acgggggcta tgtcggtcag
    12061 gcgggcgaaa ttctccacgg taaagcctcc agcattgaac acgacggtca ggcgatgttt
    12121 gccggattaa caaacccgct gccagtggcg cgttatcact cgctggttgg cagtaacatt
    12181 ccggccggtt taaccatcaa cgcccatttt aatggcatgg tgatggcagt acgtcacgat
    12241 gcggatcgcg tttgtggatt ccagttccat ccggaatcca ttcttactac ccagggcgct
    12301 cgcctgctgg aacaaacgct ggcctgggcg cagcggaaac tagagccaac caacacgctg
    12361 caaccgattc tggaaaaact gtatcaggcg cagacgctta gccagcaaga aagccaccag
    12421 ctgttttcag cagtggtacg tggcgaactg aaaccggaac aactggcggc ggcgctggtg
    12481 agcatgaaaa ttcgcggcga gcacccgaac gagatcgccg gagcagcaac cgcgttactg
    12541 gaaaacgccg cgccgttccc gcgcccggat tatctgtttg ccgatatcgt cggcaccggt
    12601 ggtgacggca gtaacagcat caatatttcc accgccagtg cgtttgtcgc cgcggcctgt
    12661 gggctgaaag tggcgaaaca cggcaaccgt agcgtctcca gtaaatctgg ttcgtccgat
    12721 ctgctggcgg cgttcggtat taatcttgat atgaatgccg ataaatcgcg ccaagcgctg
    12781 gatgagttag gcatatgttt cctctttgcg ccgaaatatc acaccggatt tcgccatgcg
    12841 atgccggttc gccagcaact gaaaacccgc actctgttca acgtgctggg accattgatt
    12901 aacccggcgc atccgccgct ggcgttaatt ggtgtttata gtccggaact ggtgctgccg
    12961 attgccgaaa ccttgcgcgt gctggggtat caacgcgcgg cggtagtgca cagcggcggt
    13021 atggatgaag tttcattaca cgcgccgaca atcgttgccg aactgcatga cggtgaaatt
    13081 aagagctatc agctaactgc agaagatttt ggcctgacac cctaccacca ggagcaactg
    13141 gcaggcggaa caccggaaga aaaccgtgac attttaacac gcttgttaca aggtaaaggc
    13201 gacgccgctc acgaagcagc cgtcgcggcg aatgtcgcca tgttaatgcg cctgcatggc
    13261 catgaagatc tgcaagccaa tgcgcaaacc gttcttgagg tactgcgcag tggttccgct
    13321 tacgacagag ttaccgcact ggcggcacga gggtaaatga tgcaaaccgt tttagcgaaa
    13381 atcgtcgcag acaaggcgat ttgggtagaa acccgcaaac agcagcaacc gctggccagt
    13441 tttcagaatg aggttcagcc gagcacgcga catttttatg atgcgctaca gggtgcacgc
    13501 acggcgttta ttctggagtg caagaaagcg tcgccgtcaa aaggcgtgat ccgtgatgat
    13561 ttcgatccgg cacgcattgc cgccatttat aaacattacg cttcggcaat ttcggtgctg
    13621 actgatgaga aatattttca ggggagcttt gatttcctcc ccatcgtcag ccaaatcgcc
    13681 ccgcagccga ttttatgtaa agacttcatt atcgaccctt accagatcta tctggcgcgc
    13741 tattaccagg ccgatgcctg cttattaatg ctttcagtac tggatgacga ccaatatcgc
    13801 cagcttgcag ccgtcgctca cagtctggag atgggggggc tgaccgaagt cagtaatgaa
    13861 gaggaactgg agcgcgccat tgcattaggg gcaaaggtcg ttggcatcaa caaccgcgat
    13921 ctgcgtgatt tgtcgattga tctcaaccgt acccgcgagc ttgcgccgaa actggggcac
    13981 aacgtaacgg taatcagcga atccggcatc aatacttacg ctcaggtgcg cgagttaagc
    14041 cacttcgcta acggttttct gattggttcg gcgttgatgg cccatgacga tttgcacgcc
    14101 gccgtgcgcc gggtgttgct gggtgagaat aaagtatgtg gcctgacgcg tgggcaagat
    14161 gctaaagcag cttatgacgc gggcgcgatt tacggtgggt tgatttttgt cgcgacatca
    14221 ccgcgttgcg tcaacgttga acaggcgcag gaagtgatgg ctgcggcacc gttgcagtat
    14281 gttggcgtgt tccgcaatca cgatattgcc gatgtggtgg acaaagctaa ggtgttatcg
    14341 ctggcggcag tgcaactgca tggtaatgaa gatcagctgt atatcgatac gctgcgtgaa
    14401 gctctgccaa cacatgttgc catctggaaa gcattaagcg tcggtgaaac cctgcccgcc
    14461 cgcgagtttc agcacgttga taaatatgtt ttagacaacg gccagggtgg aagcgggcaa
    14521 cgttttgact ggtcactatt aaatggtcaa tcgcttggca acgttctgct ggcggggggc
    14581 ttaggcgcag ataactgcgt ggaagcggca caaaccggct gcgccggact tgattttaat
    14641 tctgctgtag agtcgcaacc gggcatcaaa gacgcacgtc ttttggcctc ggttttccag
    14701 acgctgcgcg catattaagg aaaggaacaa tgacaacatt acttaacccc tattttggtg
    14761 agtttggcgg catgtacgtg ccacaaatcc tgatgcctgc tctgcgccag ctggaagaag
    14821 cttttgtcag cgcgcaaaaa gatcctgaat ttcaggctca gttcaacgac ctgctgaaaa
    14881 actatgccgg gcgtccaacc gcgctgacca aatgccagaa cattacggct gggacgaaca
    14941 ccacgctgta tctgaagcgc gaagatttgc tgcacggcgg cgcgcataaa actaaccagg
    15001 tgctcggtca ggctttactg gcgaagcgga tgggtaaaac cgaaatcatc gccgaaaccg
    15061 gtgccggtca gcatggcgtg gcgtcggccc ttgccagcgc cctgctcggc ctgaaatgcc
    15121 gtatttatat gggtgccaaa gacgttgaac gccagtcgcc taacgttttt cgtatgcgct
    15181 taatgggtgc ggaagtgatc ccggtacata gcggttccgc gacgctgaaa gatgcctgta
    15241 acgaggcgct gcgcgactgg tccggcagtt atgaaaccgc gcactatatg ctgggcaccg
    15301 cagctggccc acatccttat ccgaccattg tgtgtgagtt tcagcggatg attggcgaag
    15361 aaacgaaagc gcagattctg gaaagagaag gtcgcctgcc ggatgccgtt atcgcctgtg
    15421 ttggcggcgg ttcgaatgcc atcggcatgt ttgcagattt catcaacgaa accaacgtcg
    15481 gcctgattgg tgtggagccg ggtggtcacg gtatcgaaac tggcgagcac ggcgcaccgc
    15541 taaaacatgg tcgcgtgggc atttatttcg gtatgaaagc gccgatgatg caaaccgaag
    15601 acgggcagat tgaagaatct tactccatct ccgccggact ggatttcccg tctgtcggcc
    15661 cacaacacgc gtatcttaac agcactggac gcgctgatta cgtgtctatt accgatgatg
    15721 aagcccttga agccttcaaa acgctgtgcc tgcacgaagg gattatcccg gcgctggagt
    15781 cttcccacgc cctggcccat gcgctgaaaa tgatgcgcga aaccccggaa aaagagcagc
    15841 tactggtggt taacctttcc ggtcgcggcg ataaagacat cttcaccgtt cacgatattt
    15901 tgaaagcacg aggggaaatc tgatggaacg ctacgaatct ctgtttgccc agttgaagga
    15961 gcgcaaagaa ggcgcattcg ttcctttcgt cacgctcagg gatccgggca ttgagcagtc
    16021 attgaaaatt atcgatacgc taattgaagc cggtgctgac gcgctggagt taggtatccc
    16081 cttctccgac ccactggcgg atggcccgac cattcaaaac gccactctgc gcgcctttgc
    16141 ggcaggtgtg actccggcac aatgttttga aatgctggca ctgattcgcc agaaacaccc
    16201 gaccattccc attggcctgt tgatgtatgc caatctggtg tttaacaaag gcattgatga
    16261 gttttatgcc cagtgcgaga aagtcggcgt cgattcggtg ctggttgccg atgtgccagt
    16321 tgaagagtcc gcgcccttcc gccaggccgc gttgcgccat aatgtcgcac ctatcttcat
    16381 ctgcccgcca aatgccgatg acgacctgct gcgccagata gcctcttacg gtcgtggtta
    16441 cacctatttg ctgtcacgag cgggcgtgac cggcgcagaa aaccgcgccg cgttacccct
    16501 caatcatctg gttgcgaagc tgaaagagta caacgctgca cctccattgc aggggtttgg
    16561 tatttccgcc ccggatcagg taaaagcagc gattgatgca ggagctgcgg gcgcgatttc
    16621 tggttcggcc attgttaaaa tcatcgagca acatattaat gagccagaga aaatgctggc
    16681 ggcactgaaa gtttttgtac aaccgatgaa agcggcgacg cgcagttaat cccacagccg
    16741 ccagttccgt tggcggcatt ttaactttct ttaatgaagc cggaaaaatc ctaaattcat
    16801 ttaatattta tctttttacc gcttcgctta cccctgtcga acgtcaactt acgtcatttt
    16861 tccgcccaac agtaatataa tcaaacaaat taatcccgca acataacacc agtaaaatca
    16921 ataattttct ctaagtcact tattcctcag ata
//
LOCUS       DAJVTY010000086        16924 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVTY010000086 DAJVTY010000000
VERSION     DAJVTY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16924
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.086"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            193..2040
                     /gene="ldtD"
                     /locus_tag="OQQ66_002476"
     CDS             193..2040
                     /gene="ldtD"
                     /locus_tag="OQQ66_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1905368.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2221..2769
                     /locus_tag="OQQ66_002477"
     CDS             2221..2769
                     /locus_tag="OQQ66_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1905369.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2796..3443
                     /gene="gloC"
                     /locus_tag="OQQ66_002478"
     CDS             2796..3443
                     /gene="gloC"
                     /locus_tag="OQQ66_002478"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1905370.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OQQ66_002479"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OQQ66_002479"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1905371.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OQQ66_002480"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OQQ66_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1905372.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="OQQ66_002481"
     CDS             complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="OQQ66_002481"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1905373.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OQQ66_002482"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OQQ66_002482"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1905374.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="OQQ66_002483"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="OQQ66_002483"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1905375.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12422..13173)
                     /gene="ssuB"
                     /locus_tag="OQQ66_002484"
                     /pseudo
     CDS             complement(12422..13173)
                     /gene="ssuB"
                     /locus_tag="OQQ66_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13170..13907)
                     /gene="ssuC"
                     /locus_tag="OQQ66_002485"
                     /pseudo
     CDS             complement(13170..13907)
                     /gene="ssuC"
                     /locus_tag="OQQ66_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13972..15117)
                     /gene="ssuD"
                     /locus_tag="OQQ66_002486"
                     /pseudo
     CDS             complement(13972..15117)
                     /gene="ssuD"
                     /locus_tag="OQQ66_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15114..16073)
                     /gene="ssuA"
                     /locus_tag="OQQ66_002487"
     CDS             complement(15114..16073)
                     /gene="ssuA"
                     /locus_tag="OQQ66_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1905376.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16066..16641)
                     /gene="ssuE"
                     /locus_tag="OQQ66_002488"
     CDS             complement(16066..16641)
                     /gene="ssuE"
                     /locus_tag="OQQ66_002488"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1905377.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4248 a         4360 c         4299 g         4017 t
ORIGIN      
        1 cataacggcg cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg
       61 tttatatact gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta
      121 ttggcgttgt acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac
      181 agggggcaag ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg
      241 tgcctggccg taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc
      301 cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc
      361 acggcaataa tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg
      421 caaatcgaat ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg
      481 ttgtatgccg cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc
      541 cagcaacagc tggcagaggt ggcgattgcc ggtttccagc cgcagtttaa taaatgggta
      601 gagttactga ccgatcctgg tgttaacggg atggcacgcg acgtagtgct ctctgatgcg
      661 atgatgggct atctccattt cattgcaaat attccggtca aaggcactcg ctggctatat
      721 agcagtaaac cttatgcact tgcaacgccg ccgctctcgg tgattaacca atggcagctg
      781 gcgctggata aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa
      841 tatgcggcga tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa
      901 ctgaccggca aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc
      961 gaaatattgc aacgcacagg catgttggac ggggggccga aaattactct acctggcgat
     1021 gacacgccaa ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact
     1081 aagccgatgg acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgcgccgcc
     1141 tacgataatg aactggtgga agccgttaaa cgttttcagg catggcaagg attgggggca
     1201 gatggtgcta ttggcccggc aacacgtgac tggttaaacg taacgcccgc ccagcgtgct
     1261 ggcgtgttgg ctctcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg
     1321 atcatggtta acattccggc ctattcgctg gtctactatc agaacggcaa tcaggtgctg
     1381 gattcgcgag tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgccctt
     1441 aacaacgtag tggtaaaccc gccgtggaac gtacctccaa ctctggcacg caaagatatt
     1501 ctgccaaaag tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgt
     1561 ggctggaaca gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc
     1621 tcgaatttac cgttccgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat
     1681 aaattcaata tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg
     1741 ttcaagcgtg atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat
     1801 ctggcgaata tgctgttgca ggatgcaggc tggaatgaca aacgtatttc tgatgcgctg
     1861 aagcagggtg atacacgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac
     1921 ctgacggcct ttgttggtgc agatggtcgt acccagtatc gtacagatat ttacaattat
     1981 gatctgcctg cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa
     2041 atgaagtagt tcggggaaat cagttgtcgt aataattagc atgattgggg gcgattctct
     2101 gcagcccccg tcactgctgg ggttgagtca tcttgacgtc tgctttacgg gcggttaagg
     2161 tgcctcttgt gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc
     2221 atggacaaat tcgacgctaa tcgccgcaaa ttgctggcgc ttggtggcgt tgcactcggt
     2281 gccgccatcc tgccgacccc tgcgtttgca acactctcta ccccacgccc gcgcattttg
     2341 acactcaata atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc
     2401 tatattcagg aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa
     2461 ataaagtcca tcgacccagg attattcgac cagttgtatc gcctgcaagg gttgttaggc
     2521 acgcgcaaac cggtgcaact catttccggt tatcgttcca ttgataccaa caatgaacta
     2581 cgcgcccgca gccgtggagt agcgaagaaa agctatcaca ctaaaggcca ggcgatggat
     2641 ttccatattg aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca
     2701 ggtggtgtag gatattaccc acgtagtaac tttgtgcata ttgataccgg gccagcacgg
     2761 cactggtagc aatcgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca
     2821 ccgcattctc ccagaactgt tcattaatct ggtgtgaaca aacccgtctg gccgcactgg
     2881 tcgatcctgg cggcgatgcg gaaaaaatca aacaggaagt tgatgacagc ggcctgacac
     2941 tgatgcagat cctgctgacg catggtcatc tggaccacgt tggcgcagcg gcggaactgg
     3001 cgcaacatta cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag
     3061 gcttgcctgc gcaaagtcgt atgtttggtc tggaagagtg ccagccgctg acgccagatc
     3121 gttggctgaa cgaaggcgat accatcagca taggaaatgt gactttacag gtgttacatt
     3181 gccctgggca tacgccgggt catgtcgtgt tttttgatga tcgggcaaag ctgctgattt
     3241 ctggcgatgt tattttcaaa ggcggagtag ggcgcagtga cttcccgcgt ggcgatcata
     3301 atcaactgat ttcttcaatc aaagataaat tgctgccact gggggatgac gtgacattta
     3361 ttccgggtca cggaccatta tccacacttg gttatgaacg cctgcataat cccttcctgc
     3421 aagacgaaat gcccgtctgg taaggcacat aaaaaagccc gcttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 atgcttacag cactgccaca atcgcttcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaaccg catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcagg ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcgcct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaac gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacggtttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcattcag gctgttaatc agtgcatcga
     4381 agtcaagagt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacact cgcttaacgc
     4501 tggtattttt tgccaggaaa tcggcagcta cgcgtagtgc gccagtgccc cccggagtct
     4561 gtgccatgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gtataaaaaa aacaggacca aagtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgtta
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccttggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt ttttatgaca cctgccactg ccgtcaataa
     6181 gttctgtcaa taaaaattta cggaactatt gatgagagtt tggtgtcttt atgtgtctgc
     6241 aggcatcttt ccattcaaac taacgtttcg ctaccgtgaa agtgctgcaa agataaagat
     6301 ttagtttcaa aaagaaaaaa tatgtaacca aaagtaaaaa ttaaggaact ttgtgaacac
     6361 cgtcatattt ccatagaaac gtgatgatat ttacagcaat ttttatctat ttatatgatt
     6421 tacttatatt taaattaact aagcggaaat tttgtttctg atggaaactt tatcgacctg
     6481 gcacaaaatc ctctttcaga catccagaat gccaaaaaat agtatgaaaa ttgtgctatc
     6541 agctaagaaa aaactaatcc gcaataagaa tacgggttat tttttgtgcg taaatgttgc
     6601 aatgtaatat tccgtaacag gatgatcgtt atggcggggt tgcaaataga ttgcttgact
     6661 taacaaacag ataacttgac agaaaggata aaaaagagcc agcaggcgct ggctctttga
     6721 ggaagaaaat cagaagctgg cgttgcgcgg agtacgtggg aacggaatca catcacgcac
     6781 gttttgcacg ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg
     6841 cggaacagta ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc
     6901 catttccagc atacgctcgt ccagaacgtc cagacgttct tcacgctggg agcctccaat
     6961 gatctcaccg atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt
     7021 gaggcgcata tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt
     7081 aaagtgttct tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac
     7141 cgggttttca aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac
     7201 ctgcgcgaaa tcggcctcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg
     7261 ttcagcgaag aatttcatgt cgtctgcgcg ttcttcgaga accgctttga agacatattt
     7321 cagcatggct tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc
     7381 cagcatccag aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg
     7441 gccgaaggta taaattttgg ataatgcgca agcgtaggtt tcgccgttca actggccaga
     7501 tacggtcagg aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc
     7561 gttacgcggc aggttttcca gatccagcgt agaaacgcgg aacatttcgc ctgcaccttc
     7621 ggtatcagat gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa
     7681 gcgatgcagc gcctgcgcca gcgtatggcg aacgcgcgcg acggcaccaa tcaggtttgt
     7741 gcgcggacgc aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat
     7801 cgggtaagtg tctggatctt caacccaacc agcaacttca actttgctgg cctgaatttc
     7861 aaattgttgc ccctggcccg gcgacgccac gactttaccc gtcacaatga ccgagcagcc
     7921 ggtggtcaga cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg
     7981 tacaggatca aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc
     8041 tcggcgggta cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg
     8101 gagtacgtcg gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa
     8161 taaacacttg tccacccgta aatgggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gctttttata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgactg gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ttcttccagc caggcagcaa gtgcagctcg ctggctgttg gctaggtccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgcgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 agtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga attcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcatcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cattttacgc ttaagatcgt taatgaaata agcccggcgg cgaataccgc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaaccaaat atcccttcct gctttccaac ggtaaccgcg ttgcgcaagg
    10261 tgaactggaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gacaccttta ccacgcgttc
    10381 tggtcgcgaa gtggcgctgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actcaatgaa atgggatgaa gaacgcttcg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtttg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccactga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtc acctgccgcg actggttcca gctcagcctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgtgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccgatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctataccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgccatg atggcagcgc ggcaacctgc gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgtcg atctctccca tttccgccgt tggtacagcc agtccggcac
    11101 gccgattgtg accgtcaaag acgactacaa tccggaaacc gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatctcg ttgcagaaag gcggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgctg
    11461 ggatgcggca caaagcctgc tggcaaccta catcaagctg aacgtcgccc gacatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcag tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcgaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcagttcca
    11881 cgaagcgaac aatatgaccg atgcgctggc ggcgctttct gcggcggttg ccgcacagct
    11941 gccatgccgt gatgcgctga tgcaggaata cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgcggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggcgcg tttgcgggca gcaatccggc agcgttccat gccgaagatg ggagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tggcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatctgt acgagaagat
    12361 caccaaagca ctggcttgat aaataaccga atggcggcaa tagcgccgcc attcggggaa
    12421 tttacccctg ttttctcagg cgaatttcag attcaccacg ctgcatcacc ctttgcaaca
    12481 cttccgattc cagctccgct aatcgcaccg accctaatcg acgcggaggg gggatatcca
    12541 ctgtcaaatc cagaccaatt tttccctctt caattaataa cacccggtca gccattgcta
    12601 ccgcttcgct cacatcatgc gtcaccaaca gtacggtaaa gccgtgctcc tgccaaagtg
    12661 acacaatcaa atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat
    12721 caagcaacaa taaacccggt cgatgaatca acgctcttgc cagcaccact cgctgtttct
    12781 gcccgcctga aagtgcggca ggccattccc cggcgcgatt ctccagtcct accgcagcca
    12841 acgcccgacg cgcggcatcg cgccactggc ctttaaggcc taacccaacg ttatcaatca
    12901 ccgatttcca cggtagcaga cgcgcatcct gaaacatcat tcgcgtgtct tcctgaattt
    12961 cagccagcgg tgtggtgcct gccaacacat cgcctgcggt tggcgtttcc agcccggcca
    13021 gcaggcgcag cagggtactt ttgccaccac cgccacaaac tgacctgccg gaatatgtaa
    13081 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13141 tggtgtgccc tggttcagac gagcagtatt cataccgtgg cctccttcaa atgataagcc
    13201 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13261 agcagggcgt aaagaataat agcgactacc accacgtccg tttgcaaaaa ctctcgcgca
    13321 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13381 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcaccaggc
    13441 aggatcacat ggataaacag cggtatgccg gataacccat agctacgcgc catctccacc
    13501 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg aaacagcgta
    13561 ccgagcgcca ccagaaagat ttttgcggat tcatcaatgc caaaccacaa aatcaccagc
    13621 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagccgt
    13681 tctccccagc gagatagccc gctaatcagt cccaaaatca accccagcga tccgccaatt
    13741 gaaaagccaa tcagcgcccg ctaggaactg atcgccagat gctgccacag ttcgccgctg
    13801 gcggagagcg tccagaacgc cgtcaccacc ccttccggtg agggcaaaat acgcgtcgac
    13861 agccagccaa ctgaggaggc cagttgccac accgccacga tgcccaccgg taaaaactag
    13921 ggggcaacgc gcaataacca cttcttcact ggcgttgcca ttatttcttc cttagctttg
    13981 cgcgacttta cgggggataa aatcattcgc caccgcttcg ccttgcggat tcagcggctg
    14041 cggctgggga atttccggaa tggcgacatc cagatgtggg aacagcaact cgccgacccg
    14101 atacgcttct tccagatgcg gatagcccga aagcacaaag ctgtcgatgc caagcgcggc
    14161 gtattcgttg attcgcgcag cgaccgtagg accatcgccc accagcgccg ttccggcacc
    14221 gccgcgcact aagccaacgc ccgcccataa attggggctg atctccagat tgtcgcgctt
    14281 gccgttatgt aacgccgcca ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc
    14341 ctgtgctttg gcgatagttt catcatcaag atgcgagatt aaccgctcgg cggcctgcca
    14401 cgcttcgtcg ttagtttcac gaacaatcac atgcagacga ataccgaaac gaattttgcg
    14461 tccatgcgcg gcagctttcg cccgcacttg ttcgatcttc tcttttacta gttctggcgg
    14521 ttcgccccag gtgaggtaga gatcaacttg ttctgccgcc agctcctggg cgacgtctga
    14581 cgatccgcca aagtaaagcg gaggataagg ctgttgaatc ggcgggaaga gcagttttgc
    14641 tccgcgcaca tgaatatgtt taccgttgaa attgacggtt tcccccagca ataagcgccg
    14701 ccagacctag gtaaattccg ccgaggcttc gtagcgctcg ctatgatcaa ggaacacgcc
    14761 gtcgcctgcc agctcttgtg gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt
    14821 tgagagacgg tcaagcgtgg cggcctggcg agcggcaacg gtaggtgagg ttacgctggg
    14881 gcgcagggcg acaagaaact tcagccgctg cgtcaccggg atcatcgacg ccgccaccag
    14941 ccacgcatct tcacacgatc gccccgttgg gatcagcaca ccggtatagc ccagacgatc
    15001 cgccgcttgc gcaatttgtt gcagataacc gtgatcaacc gggcgtgaac cttcttccgt
    15061 tcccagatag tgcccgtcac cgtgggtcgg taaaaaccag aacatattca gactcataat
    15121 tgttttcctt ccagttgagt gggctgccag atgcgctggc gaatatcgac ttttttcggc
    15181 accagacgat tttcataaaa cagatctgcc gtttgctgct gtaaggcggc aacctcggcg
    15241 ttaaccggtt tgatggtggt aggagggcga tgatctaagt aagaggcaat caccggtgcc
    15301 ggtaagccca tcgtttttgc cagtaaagcg atgctttgct cgcgctggct gcgggttaac
    15361 gcatcggcct cactaaaggt tgccagtacg ccctgaataa aagcgccgtt tttttctgca
    15421 tagggacgag ctgccagata aaacgatcca gtttgattga gatcggtgcc gtctttcagc
    15481 acccgcacgc cgccctgtaa taatgcagca gagtagtagg gatcccagat agcccaggcg
    15541 tcaacgttac cttgctggaa cgcggcgcgg gcatcagcgg gcgtcaggta agtgggttgg
    15601 atatcggtaa acttaagtcc ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg
    15661 gaacctttct gaaaggcaac tttgtgacct ttaagatcgg ctacggtttt gatcgggctg
    15721 ttttctgcca ccagaatcac ttcggctttg ggcttcggtg gctcgacgcc cacatatacc
    15781 aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact gccgagatca
    15841 atactgccaa cgtttaaggc ttccaacatt tgcggacccg cggggaactc cacccaggag
    15901 atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccag taccatacca
    15961 atactgcctt tctgatagcc tatacataac gcttcaggcg aggattctgc agcaaccgca
    16021 atcgtagaga cgctaagcaa tcccaccagc gccagtttaa tgatgttacg catgggcatt
    16081 acctcgcaga gacagaaggt caggaacctg aacatcgcgg cggtgcaatg cctgccagaa
    16141 agtttctagc gcggtatcaa gacgggtttg cagatttggc gtgaactggg gtctgtgatg
    16201 gtaatcaatt acttgtgagt catcggcaaa cacgccgtgc aggatctcct gagctttcag
    16261 tgcgcttaaa actggtttaa gggcataatc gaccgccagc agatgggcca cggtaccgcc
    16321 cgtcgccagc ggtagcacca ctttgccttg caaagcgcgc tctggcagca ggtcgagcag
    16381 ggttttcagc gcgccggaat aggcggcttt atacacaggt gtagcgacta tcagtccatc
    16441 agcctgttgc agctgttcgg tgaaggtctt gagtgccgga ctatcgaaac gagcataaag
    16501 cagatcttcc gggacgaagt tttgcagatt ccagtgataa acctctacat ccaggccatt
    16561 tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaagc gaggactacc
    16621 cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttctttt tttgttaaca
    16681 ttgataacaa ttcggtcagt ctgtcggaga gacaagaaaa tgccaaatat aaattttgtg
    16741 tatctttttc tgaaaataaa ctaaagaaag ggctttatat atcacgcata tttatttgtt
    16801 ggatagtcat tagatgttga atcaatgaat tactcgttca gttaatattt aaatattcat
    16861 gaaatctata aattaaagat ttgtcactta ttggatttag tatttttgat ttaaatgacg
    16921 gtca
//
LOCUS       DAJVTY010000087        16809 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVTY010000087 DAJVTY010000000
VERSION     DAJVTY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16809
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.087"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..270)
                     /locus_tag="OQQ66_002489"
     CDS             complement(<1..270)
                     /locus_tag="OQQ66_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1905378.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKI"
     gene            complement(570..664)
                     /locus_tag="OQQ66_002490"
     tRNA            complement(570..664)
                     /locus_tag="OQQ66_002490"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Sec,seq:tca)
     gene            957..2339
                     /locus_tag="OQQ66_002491"
     CDS             957..2339
                     /locus_tag="OQQ66_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS1905379.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2349..4640
                     /gene="yicI"
                     /locus_tag="OQQ66_002492"
     CDS             2349..4640
                     /gene="yicI"
                     /locus_tag="OQQ66_002492"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1905380.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4693..6402)
                     /locus_tag="OQQ66_002493"
     CDS             complement(4693..6402)
                     /locus_tag="OQQ66_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1905381.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLLMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6523..7914)
                     /gene="xanP"
                     /locus_tag="OQQ66_002494"
     CDS             complement(6523..7914)
                     /gene="xanP"
                     /locus_tag="OQQ66_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1905382.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8194..9399
                     /gene="gltS"
                     /locus_tag="OQQ66_002495"
     CDS             8194..9399
                     /gene="gltS"
                     /locus_tag="OQQ66_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1905383.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9433..11514)
                     /gene="recG"
                     /locus_tag="OQQ66_002496"
     CDS             complement(9433..11514)
                     /gene="recG"
                     /locus_tag="OQQ66_002496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1905384.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11520..12209)
                     /gene="trmH"
                     /locus_tag="OQQ66_002497"
     CDS             complement(11520..12209)
                     /gene="trmH"
                     /locus_tag="OQQ66_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1905385.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12216..14324)
                     /gene="spoT"
                     /locus_tag="OQQ66_002498"
     CDS             complement(12216..14324)
                     /gene="spoT"
                     /locus_tag="OQQ66_002498"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1905386.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14343..14618)
                     /gene="rpoZ"
                     /locus_tag="OQQ66_002499"
     CDS             complement(14343..14618)
                     /gene="rpoZ"
                     /locus_tag="OQQ66_002499"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1905387.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14673..15296)
                     /gene="gmk"
                     /locus_tag="OQQ66_002500"
     CDS             complement(14673..15296)
                     /gene="gmk"
                     /locus_tag="OQQ66_002500"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1905388.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15554..>16809
                     /gene="ligB"
                     /locus_tag="OQQ66_002501"
     CDS             15554..>16809
                     /gene="ligB"
                     /locus_tag="OQQ66_002501"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1905389.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLV"
BASE COUNT         3813 a         4287 c         4536 g         4173 t
ORIGIN      
        1 gattttttct tccttttttg cctcccctgg atcgcctcca gcagcgagga gtttcctggc
       61 ttcactgcgt ttctcgcgag catcggctaa cgttacatcg gggtatacgc caatagaaag
      121 catcttttct ttaccagcaa agcggtactt catgcgccag tatttagaac cattagtgtt
      181 aatcagtaga tacataccgc cgccatcagc aagcttatag ggtttgtctt tgggttttgc
      241 ggttttcact ttgatatcgg ttaatgccat gacatcctcc ggcttttgat gaattcatct
      301 tcctgagggg gatttctgcc agtaactaac ctgatacccc caatacaccc cttgtataac
      361 aaagatattg ttaaaagccg ttgttatgta tcatattttt cctattatgg gggtgcttgt
      421 atttcaatag gaaataacac cccctaaagt acccccaaaa gaaacttgat atggggtgaa
      481 gtaggttgac ctcaggatac aaataaatac tgatatacaa gggattagaa gagatttatt
      541 gacattggga gagttcagaa gaggtaaact ggcggaagat cacaggagtc gaacctgtcc
      601 gggaccgctg gcggccccaa ctggatttga agtccagccg cctcaccgga gacgacgatc
      661 ttccgcgcct cgattgctac atggaggcgg ggcgcattat agctacttcc ttgagtttct
      721 acatccccca gatcagtttt ttccgcctaa tacacgcagc gtattatcac gaaagcatta
      781 tagttcatat aaaacccata ttttacaatg acatatgagc ggaattaacg ctaccacgat
      841 cacataaaac aagaatcata aattaataac cagatatcgg aatattcgct ctcacaggga
      901 tggtttacaa aatgcgttat cggtctcagc acccctatag catcaaggaa aagcagatga
      961 agagtgaagt gttgtccgtt aaagagaaaa ttggttatgg catgggagac gccgccagcc
     1021 acattatttt cgataacgta atgttatata tgatgttctt ttataccgat atttttggca
     1081 ttcctgccgg ttttgtcgga accatgtttt tggtcgctcg tgcactggat gcgatttccg
     1141 atccttgcat ggggttgttg gccgatcgaa cgcgctctcg ctggggtaaa tttcgtccgt
     1201 gggtactgtt tggcgcactg ccattcggga tcgtctgtgt actggcctat agcacgccag
     1261 atctcagtat gaacggcaaa atgatctatg cagcaattac ttacacccta cttaccttac
     1321 tttataccgt cgtcaatatc ccttactgcg cattgggtgg tgtaatcacc aatgacccga
     1381 ctcagcgtat ctcgctgcaa tcctggcgtt ttgtgctggc gaccgcggga ggcatgcttt
     1441 ctactgttct gatgatgcca ctggttaatt taattggcgg tgataataaa ccactcggtt
     1501 tccagggcgg tatcgcggtc ctttccgtgg tggcattcat gatgctggca ttttgtttct
     1561 tcaccactaa agaacgcgtt gaagcaccac ctacaacaac gtctatgcgg gaagatttac
     1621 gtgatatctg gcaaaacgac cagtggcgga ttgtcggttt actaaccatt ttcaatatcc
     1681 tggcggtgtg cgtactcggt ggggcgatga tgtattacgt cacatggatt ttgggcacgc
     1741 cggaagtgtt tgtcgctttt ctcaccactt attgcgtggg taacctgatt ggttccgcac
     1801 tggcaaaacc tctgaccgac tggaaatgta aagtcactat cttctggtgg acgaacgccc
     1861 tgctggcagt gattagcctc gcgatgttct ttgttcccat gcaggccagc atcactatgt
     1921 ttgtcttcat cttcgtgatt ggtgtgttgc atcaactggt gacacctatc cagtgggtaa
     1981 tgatgtccga taccgtcgac tacggcgagt ggtgcaatgg taaacgcctg accgggatca
     2041 gttttgctgg cacgctgttt gtgctcaaac tggggttggc cttcggcggc gctcttatcg
     2101 gctggatgct ggcttatggc ggatatgatg cggcagaaaa agcgcagaac agcgccacga
     2161 ttagcatcat tattgcgcta ttcacgattg ttccggcgat ctgttatttg ctgagcgcga
     2221 ttatcgctaa acgctactac tcactcacga cgcacaatct gaaaaccgtt atggaacagc
     2281 tggctcaggg taaacgccgt tgccagcaac aattcacctc tcaagaagtg cagaactaag
     2341 gaacggcaat gaaaattagc gatggaaact ggttgattca acctggcctc aatttgattc
     2401 acccgcttca ggtgttcgag gttgaacagc aggataatga aatggtggtc tatgctgccc
     2461 cccgtgatgt gcgtgaacgt acctggcagc ttgatacgcc tttatttacg ctgcgctttt
     2521 tctccccaca ggaaggtatt gtcggtgtgc ggattgagca ttttcaggga gcgctgaata
     2581 acggtcctca ttatccgctc aatattttgc aggacgtgaa ggtcacaatc gaaaacacag
     2641 aacgttatgc tgagtttaaa agtggcaact taagcgcgcg tgtcagcaaa ggtgagttct
     2701 ggtcactcga ttttctgcgc aacggtgaac gtattaccgg tcaggacacg aataatcaac
     2761 gaaattatat gtttgagcgg cttgatcttg gcgttggcga aacagtttat ggtctgggag
     2821 agcgttttac tgccctggtg cgcaatggtc agacggtaga gacctggaac cgggacggcg
     2881 gcacaagtac tgaacaggcg tataaaaata tcccgttcta tatgactaac cgtggctatg
     2941 gggtgctggt caatcacccc cagagtgtct cttttgaagt gggatcggag aaagtctcca
     3001 aagtgcagtt cagcgttgag agcgaatatc tcgaatactt tgttatcgac ggtccgacgc
     3061 cgaaagcggt acttgatcgt tatacccgtt ttactggtcg tccggcgctg ccgcccgcat
     3121 ggtccttcgg cctgtggcta accacttcat ttaccaccaa ctacgacgaa gcgacggtaa
     3181 acagctttat cgatggtatg gcggaacgca atctgccgct gcatgttttc cactttgact
     3241 gtttctggat gaaagccttc cagtggtgcg attttgagtg ggacccgctg actttcccgg
     3301 acccggaagg gatgatccgc cgcctgaaag cgaaagggct gaaaatctgc gtctggatta
     3361 acccctatat cggtcaaaaa tcccccgtct ttaaagagtt acaagagaaa ggctatttac
     3421 tcaaacgccc ggacggttcg ctatggcagt gggataaatg gcagccaggt ctggcgattt
     3481 atgactttac caatccggat gcctgcaaat ggtacgccga caaactgaaa ggtctggtcg
     3541 cgatgggcgt tgattgcttt aagaccgact ttggcgaacg tatcccaact gatgttcagt
     3601 ggtttgacgg ttccgatccg cagaaaatgc ataaccatta tgcgcacatc tacaacgaac
     3661 tggtgtggaa cgtgctcaag gacaccgttg gtgaggaaga agctgtcttg tttgcccgct
     3721 cggcctccgt cggtgcgcag aaattcccgg tacactgggg tggcgattgt tacgctaact
     3781 acgaatcaat ggcggaaagc ctgcgcggtg gtttgtctat tggcctttca ggttttggct
     3841 tctggagcca ctatatcggc ggctttgaaa ataccgctcc ggcgcacgtt tacaaatgct
     3901 ggtgcgcgtt tggtttgctc tccagccata gccgtttaca cggtagcaaa tcttatcgtg
     3961 tgccgtgggc ctacgatgat gagtcctgtg atgtggtgcg cttcttcacg caactgaaat
     4021 gccgcatgat gccgtatctg tatcgtgaag ctgcgcgtgc aaacgcgcgg ggtacgccga
     4081 tgatgcgggc catgatgatg gagttcccgg acgatccggc ttgtgattac cttgaccgtc
     4141 aatacatgtt aggcgacaac gtgatggttg cgccggtgtt cactgaagcg ggcgatgtgc
     4201 agttctacct gccggaaggt cgctggacac acctgtggca caacgatgag ctcgacggtc
     4261 gttgctggca taaacagcag cacagcttcc tgagtctgcc catttatgtg cgtgataaca
     4321 ccctactggc gctgggcaac aacgatcaac gtcccgatta cgcgtggcac gaaggcacgg
     4381 cattccacct ctttaatctg caagacgggc atgaagccgt ctgtgaagtg cccgctgctg
     4441 acggatcggt gatctttact ttaaaagcag cacgtactgg caacacgatt actgtgactg
     4501 gtgcgggcga ggcgaagaac tggacactgt gcctgcgcaa tgttgtgaaa gtaaatggtc
     4561 tgcaagacgg ttcgcaggct gaaagtgagc aggggctggt ggtgaagcct caagggaatg
     4621 cgctgacaat tacgttgtaa tctattgatg gaatgccggg tacgtctgag tcgtacccgg
     4681 cgagattgat gtttactgca ccagctctgc cgacgtaact tcaccgctgg aaacgatgcc
     4741 agcattcatg gtttgagtca cttgctgctt tgcggcgttc acggcatgca attgcccgct
     4801 aaccgtaggt ttcagcgggg cattggcctg aatatctcca ctggcggtaa gctgaatatt
     4861 gccgtcgcca gtcaacggta attcaggcca tccccattgt tgcaaaatat tcaccggcac
     4921 accgcgtcca ttcaggctga tatgggtctg acgttgtggc gtttgtgaaa cactggcagt
     4981 ggcttccaga atgccttttt cagtaaatgc actcagttcg ctgatattca ccgtgctgct
     5041 gttggcggtc agcgccagcg acgggcgacg aacatcaaca cgattgaatg tcgcggcggc
     5101 ggcattcaga ttcgccgagc cactccagac gccccattta tgatcggtaa ccagcgtcag
     5161 gttggcaccg taaccatcga gcgtggtgag ctgccacggg aagtcagggt cgatatcaat
     5221 gatcagattg cggctggcgc taaatctctt cagttgcagg ctgtttaacc aaccgggtgt
     5281 cgtttccatc cacaactgtt gccagttttt cggcaaggta tattccagcc cggcaattgc
     5341 cgcatcatca aggatcaacg ttttcccgtc acgcagccag ttccctgacg ttctgaccat
     5401 acccccttcc cagcggctgg tgaactggcg cagcgctacg ccctgcgggg aaaattccgc
     5461 gtttataatc gggtcaaata aatgcagcga accataaatg aactcgctag cgttcatcaa
     5521 cagtttgcct tcttgtgtct gccagtcatc tttactgaag gtcatgttgc gcaagctgag
     5581 atcgaggtcg gtcaccgccc agtccggacc ttgcaaatga gcatcgatca cttccaggcg
     5641 accaatctgc aacgacggga cagagcgtaa tggcgcaaag aagtcggtta gcgatttttc
     5701 gctttgtaga cgtatatccg ccatgcgcag gttttccact tgccagctgc cgtcggcgtt
     5761 acgctgcgcg tttccggtta atgtcccgcg ggcgatgtcg gcacccaggt tagtcagcgt
     5821 aacgcgatcg ttatcaatac tgccttcaat cagtacattg gtggcaggaa catcattgag
     5881 cgaaagcgat ccggcactaa actgaatctg cgccttcgta cccagcactt taccggcttt
     5941 tggtgaccac ggaactacgc cgccatttac ccgctgcgcg ctcagtttcc attcgctatt
     6001 cgggctatta aacgccatat cacgcagttg cagacgatcg gctttgaacg gtagcggcgc
     6061 ggtctggtcg gtgagattca gcgtcccgtt ttccagcagg atggtatcga catggcgtgg
     6121 ttcggtcagt tgccgactgc ttagcgcaat gtcgacactt ttggccacca gggtcgcggg
     6181 ctgaccatca cgaccaaacg tgacattctc cagcacgata tgagatggcg cggaaaaacg
     6241 gtgatccatc gccccgaagg ccagatgata gtcgctattc tcggaaaccc atgcgctgat
     6301 atgttctgct ccccagcggg tttgcagaag aaaatagagg ccagcgatca ccactaacag
     6361 agcgatgaga atgtagagaa gcagcttccc aataaatttc atggtcttcc attccgtgaa
     6421 atgcacataa gagagttatg cacgatttac gcgcaatcct caaggcggga atggtgaaag
     6481 aggtgagact gcggcggtaa ttatcttacc gccgccagtg aattactgtt tttctggtgg
     6541 gaaaatcaga ttcagcacga tggcagtaat accgcccgcg gcgatcccgg aggagagcag
     6601 gtttttcagc cattcagggg caaactgcaa aatcagcggc tgctgagaca cgcccagacc
     6661 aaccgccagc gacagcgcga taatcagaat cgcccgacgg ttcagcggct cacgagaaac
     6721 gatacgcaca ccggaggcgg cgatggtgcc aaacattaca agcgttgcgc cgcccagaac
     6781 cggttctgga atgtgttgta caaaaccgct cactgccggg aacagaccca gcacgatcaa
     6841 catcagcgcg acgacaaaac cgacatagcg gctggcaaca ccagtcaact ggatcactcc
     6901 gttgttttgc ccgaagcagg agttcgggaa ggtgttaaac accgccgaaa caaacgagtt
     6961 caggccgttt gccagcacgc cgcctttcag gcgtttcatg tacagcggac cggacactgg
     7021 ctgttcggaa acgtcagagg tcgacgtgat atcgccaatg gtttccagcg aagtgatcat
     7081 aaagaccagc atcagcggca gcagcagact ccattcaatg ccaagaccgt aatagagcgg
     7141 cgttggcacc ataatcagtt cttgcgtcat cggttcgttg ctttctggca acatgcccat
     7201 aaaccacgcc agcgcatatc cggccgccat cgcaatcacc agtgaggcca cgcgtaagta
     7261 agggttacgt tgacggttaa gcaggataat taaggctaag accacgcctg ccagcagcag
     7321 atttttcggt gcgccgaagg tgttatcgct catggctgcg taaccgccgc caatggacgt
     7381 taacccaacc tgaattagcg acaggccgat aatcatcacc acaacgccag aaaccagcgg
     7441 cgtaataatg cggcgcgcca gatgcagaac gcgggagatc accatctcgg tgcaacttgc
     7501 cagcatcaac gtgccgaaca aagccgccat catggtagga acatcagcac caccggtttt
     7561 cagcgcggta ccgcccataa tcagcggggc aacaaagttg aagctggtgc cctgaataga
     7621 caacagcccg gagccaaccg gaccccaggt cttaatttga ataatcgatg ccacaccgga
     7681 ggcaaacagc gacatactaa taatgtgttg cgtgtcttgt gccggtaaac ccagcgcctg
     7741 gcagattaat agcgctggcg tgatcaccgc aacgaacatc gccagcagat gctgacaggc
     7801 ggcaaacagg gtttgaggaa gcggcggacg atcttcaaga cggtaaatca gttcgctgtt
     7861 ttgagtctgc gcaaccggtt gcgcattttc tgactcgagg gtggaaacag acatcgaaaa
     7921 caatcccgtg gtagcaaagc gggcatttta gctgaccgaa agagaaaagc aaacgtttgc
     7981 cacatcctca tctaaatatt taaatgagat aaatgcactt ttttatataa cttttggtat
     8041 ttttctgctt aaaatccatg ccatttgttc attaacagct gagaatttga cccaggaaag
     8101 cataactcga tacccgtcat agttcatgaa gttgccactg cgcgatgaag tatgacgagt
     8161 atgaaagagt gatgcggata caaaggagta actatgtttc atctcgatac tttagcaacg
     8221 cttgttgccg caacgctgac gttgctgctc gggcgtaagt tggtccattc cgtctccttt
     8281 ttgaagaaat acaccatacc ggaacctgtt gcgggtggtt tgttggtggc gctggcgcta
     8341 ctagtactga aaaaaagcat gggctgggaa gtcaactttg atatgtccct gcgcgatccg
     8401 ttaatgctgg ctttcttcgc caccattggc ctgaacgcca acattgccag tttgcgtgcc
     8461 ggtgggcgtg tggttggcat cttcttgatt gtggttgttg gtctgttggt gatgcaaaat
     8521 gccattggca ttggtatggc tagcctgtta gggcttgatc cgctgatggg gctgttggcc
     8581 ggttctatta cgctttccgg cggtcacggt acgggcgctg cgtggagtaa attgttcatt
     8641 gaacgttatg gcttcaccaa tgcgacggaa gtggcgatgg cctgtgcaac gttcggtctg
     8701 gtgctgggcg gcttgattgg cggtccggtg gcgcgctatc tggtgaaaca ctccaccacg
     8761 ccgaacggta ttccggatga ccaggaagtc ccgacggcgt ttgaaaagcc ggatgtggga
     8821 cgcatgatca cctcgttggt gctgattgaa actatcgcgc tgattgctat ctgcctgacg
     8881 gtggggaaaa ttgttgcgca acttttggct ggcactgctt ttgaactgcc gaccttcgtc
     8941 tgtgtactgt ttgttggcgt gattctgagc aacggtctgt caataatggg cttttaccgc
     9001 gtctttgagc gtgcggtatc cgtgctgggt aacgtaagct tgtcgttgtt cctggcgatg
     9061 gcgttgatgg ggctgaaact gtgggagctg gcttcgctgg cgctgccgat gctggcgatt
     9121 ctggtggtac agaccatctt catggcgttg tatgccatct tcgttacctg gcgcatgatg
     9181 ggcaaaaact acgatgcggc agtgctggct gcgggtcact gtggttttgg cctcggtgca
     9241 acgccaacgg caatcgccaa catgcaggcg atcactgaac gctttggccc gtcgcacatg
     9301 gcgtttttgg tggtgccgat ggtcggtgcg ttctttatcg atatcgtcaa tgcgctggta
     9361 attaagttgt atttgatgtt gccgattttt gccggttaac cgatgaagcg gcggtcttct
     9421 caccgccgcc ttttacgcat tcgagtaacg ttctgtctcc ggcatccagc gttctatcaa
     9481 ggcttttgcc tgttgtgggt aacgttcgtg aatatgtcgc gccaggcgct gaacttccgg
     9541 gatcatcgcc tgatcgcgca gtaaatccgc cactttaaat tcagcattac ccgtctgacg
     9601 cgtgcctaac aattcgccag ggccgcgaat ctccagatct ttttgcgcaa tcacaaaacc
     9661 gtcgttactg tcgcgcagca cttgcaggcg aatttgcgcc gttttagaaa gcggcgtttt
     9721 gtagagcagc acgcagtgag aagccaccgc gccacgacct acgcgcccgc gcagctggtg
     9781 taactgcgcc agacccagac gctcagggtt ttcgataatc atcaggctgg cgttaggcac
     9841 atcaacaccg acttcaataa cggtagtggc aaccagcaga tgtagctcac cttgtttaaa
     9901 cgacgccatc accgcctgtt tctcggctgg tttcatccgc ccgtgtacca ggccaacatt
     9961 caattctggt agcgccagtt tcaactcttc ccaggtagct tccgccgcct gcgcttccag
    10021 taactctgac tcttcaatca acgtacaaac ccagtacgcc tgacgacctt cagttatgca
    10081 ggcatggcgc acgcggtcaa tgatgtcggt acggcgggta tcaggaatag cgaccgtagt
    10141 cactggcgtg cggcctggcg gcagctcatc tatcaccgag gtatcgagat cggcatacgc
    10201 cgtcattgcc agcgtgcggg ggatcggcgt ggcggtcatg atcaactgat gcggatggaa
    10261 gccttgctgc tggcctttct cccataacgc cagacgctga tgcacgccaa agcgatgttg
    10321 ttcgtcgata atcaccagcg ccagaccgtt aaactgcacc tgttcctgga agatggcgtg
    10381 cgtaccaaca atcatctgca cctgaccgct ggcgatggct tcctgctgag acagtcgcgc
    10441 tttgcctttc tgcttaccgg cgagccagcc cacttcgata ccgagcggtt caaaccagtt
    10501 gcggaagtta ttggcgtgct gttcggcgag taattcggtt ggtgccatca atgccacctg
    10561 tttgccgtgg gcaatcgcac gcagcgcggc gagggcggcg accagcgttt taccggaacc
    10621 tacatcgccc tgtaccaggc gcatcatcgg cacatccagc gccatatcgc gctcgatctc
    10681 cgccactacg cgtgcctgtg cgcccgttgg cttgaacggt aaggctgcga ggagtttatt
    10741 tttcagcgca tcattggcgc tcagcggctg ggcatgaaaa cgctgtgctc cggcacgtaa
    10801 ggctaacatg ctgaggttgt gcgccagcag ttcttccaga atcagacgac gttgcgccgg
    10861 atgctgcccg gtttccagat cgctaagctg tagcgtcggt ggcgggcggt gcaaagtgcg
    10921 caacgcttcc ggtagcgtca tcattccttg tgacagttcc ggcggcagga gttcttcaat
    10981 ggcgcaggtg tcgagcagat ccagcgcctg gtcggttaat ttacgcagcg tggcctgctt
    11041 tacgccttcc gttgttggat aaaccggcgt gagcgtttcc tgtaattctg gcgtgctgag
    11101 atcgccctgc acgcggtatt ccgggtggat catctccgca ccatatttac cgcgctttgc
    11161 ttcgccataa gccagtacac ggcggcccgt cgccaggcta tttttcattg ccgcgctgaa
    11221 attgaaaaag cgcatggtga ggatgccgga accgtcgctg atctggcagg tcatcatccg
    11281 ccgaccgccg aaggagatat tgcagttcag cacttcgcct tccaccgtgg cataaacgcc
    11341 cggcagtagt tctccgatgg ggtagagatg ggtgcgatct tcgtagcgca ggggaaggtg
    11401 taagagtaga tcctgcacgg tatgcaggtt gattttcgcc agtttgttac taagtgctgc
    11461 gccaacgccc gttagggaac tgagtgggac agcatctaac aggcgacctg tcatggcact
    11521 taccctgcag cctgcatagt agcccaccag tcggcatcag cttcgatctc gccttgctga
    11581 ttgacgtggg gataaggcag gcctttgcgt tttgcgactt tcgccagcac cggatagccg
    11641 ccttcaaaca acaggcgttg ttgctctgct tccggcaaca tgctgttttc acgcaggtac
    11701 atgcctgcat tttgccgctg acgctgggct tcgtaaagaa tgagggctga ggcaacggaa
    11761 acattcagcg actgcaccat gccgatcatc ggaatgatga tgtcctgatc cgccagggcc
    11821 aatgcttcct gcgtgatgcc cgttttctcc tgtcccatca aaatgcaggt cgggcgagtg
    11881 taatcaattt cgcggaaatc gacagcgtta tcagaaagat gggttgccag aatctgcatg
    11941 ccctggcctt tgagatgagc gacggcatcg ccaatggtgc ggtgtgtttt cacctgtacc
    12001 cagctgttac tacccgccgc tgccgaagcc atggtgcgca tgcggctacc aggccagacg
    12061 gcgtgaactt catgtacgcc aacggcatct gcggtacgaa taatcgcaga aacgttatga
    12121 ggtttgtgga cctgctccat gcagacggtc agatcaggct gccgcctggc gagcatttcg
    12181 cagatgcgtg cataacgtgt tgggttcata aaacattaat ttcggtttcg ggtgacttta
    12241 atcacgtctg gcatcacgcg gattttgcgc atgatattcg ccagatgcac acggtcacga
    12301 gcggtcagac gaataaaggc gctgtagacg cgaccatctt tctcttccgt attcaaactt
    12361 tgaatattcg aagtcgtggt gttaattgcc gccgtcaggt ttgccagcgc accctgatga
    12421 ttgaacatct ccaccttgat ttcggtgatg aactcctgcg ccgtctcttt atcccattcc
    12481 acagccataa acttctctgg ctctttctgg tagccacgga tgttacggca ggattcatgg
    12541 tggatcacca gacctttacc ggggctgacg tgggcgataa tcgggtcgcc aggaataggg
    12601 cggcagcatt tcgcaaaggt gatcagcacg ccatccgcgc ctttgatcgg cagatgcccg
    12661 tggctttgag tcgccggtgg aatggaggcg tccccatgtt gtagattttt cgcgaccacc
    12721 acgctcattg cgttaccaag gccgatttct gccagcagat cgtcaagcgt tgccagcttc
    12781 atgcgatcca gctcgcgctg aatattttcc tgcgggattt cattgagctt acggctacca
    12841 cccaaagcat ggttgagcag acgacggccc aggcttacag aatcatcacg cttgaggttt
    12901 ttcagcaact gacgaatttt ggcgcgcgct ttcgagctaa cgacaaagtt cagccaagcg
    12961 gcattcgggc gagcgcccgg agcggtaatg atttcaacgg tctgaccgct agtaagcggc
    13021 tgcgacagcg ggtaaggctg gcggtcgacg cgtgcgccca cgcaggcatg gccgatatcg
    13081 gtatgcactg cataagcgaa gtcgacgggc gttgcaccgg caggcagctc gacaatgcgc
    13141 ccttccggtg tgaaaacgta aatctcatcc gggaagagat cggatttaac gctctcgata
    13201 aattcaaacg aactaccggc gctctgttgc agctccagca ggctttgcat ccagcgctgg
    13261 gcgcgtattt gtgcggtagt actggtttcg ccgtgctctt tataagccca gtgcgcggca
    13321 acacccatct ccgccatctg atccatatct tcggtacgga tctggacctc aaccggcacg
    13381 ccgtgcgggc caatcatcga ggtgtgcaac gactgatagc cgttcgcttt tggaatggcg
    13441 atatagtctt tcacgcggcc cggacgcggc ttgtacaggc tgtgcatctg gcccagcacg
    13501 cgataacagg tgtcagaatc attgacgatc acgcggaaag cgtagatgtc catgattgag
    13561 tgaaaacgct gctctttgag caccattttg cagtaaatcg aataaagatg cttttcgcga
    13621 ccactgacgc ggcacggtat tcccgcttcc tgcaaacgcc cttcgatttc agaaagaatc
    13681 ttctggatca tctctttacg gttgccgcgc gcggctttca ccacttcttt gattacgcga
    13741 taacggttgg gatacagcgc ctcaaaaccc agctcttcga gttcggtttt aatgtggtgg
    13801 atacctaaac ggtgcgccag cgggctgtaa atttcgagag tttcacgggc gatgcggcga
    13861 cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt gggtacggtc ggcaagtttg
    13921 atgaggatga cgcggatatc ctgcaccatc gccataatca tcttgcgaaa gttttcggcc
    13981 tgcgcctctt tcttatcgcg gaacttgagt ttatcaagtt tcgacacccc ctctaccagc
    14041 tcggcgacgc ttttaccaaa aagctgttcc atatcctggt aggtggcggg agtatcttca
    14101 atcacgtcat gcagcagcgc cgccatcagc gtttcatagt cgagtttcat ctcggccaga
    14161 atgcaggcaa ccgctaccgg gtgcgtgata tagggttcac cgcttgaacg tgtttgcccc
    14221 tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac gcttgatttg gtcttccggc
    14281 aggtaggttt gaatcagttg attcaggctt tcaaacagat acaagggcga cccgctttgt
    14341 gattaacgac gaccttcagc aatagcggta acggcttgta attcagcggc ttcctgctct
    14401 tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt tgatcagacc ttcttcgatt
    14461 tcgcgcagcg cgattacagt ggttttatcg ttttcttccg gtaccagcgg atcctttccg
    14521 cctacctgca tctgacgagc gcgacgcgcg gcgaccagta ccaggtcaaa acggttacca
    14581 attttctcta cagcgtcctg aacagttacg cgtgccatac ttaaaaagct ccacaggtga
    14641 agaaatgact gggcatgata ctgaaatcag gttcagtctg ccaacaattt gctgattaaa
    14701 gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt cggcgcgaat aatggtcttc
    14761 aaatcggtca acgcggtatc gaagtcatca ttcacaatca ggtaatcata ttcgtcgtaa
    14821 tggctcattt ctgcaacagc ttgcgccata cgctttgcaa tgacctcttc gctgtcctga
    14881 ccgcgaccgc gtagacggcg gtccagttca attttagacg gcggtaaaat aaagatactc
    14941 cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcgccct gccagtcgat atcgagaaaa
    15001 acatcgacac cggtcgccag tacttgctca atggcctcac gcgaagtgcc atagtaatta
    15061 ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa tcatttcttt aaattcatca
    15121 tgattaacaa agaaataatg ttcaccgtgg acttcaccag gacgcggttg gcgtgtggtg
    15181 tgtgaaacag aaacctgggt gtcatacaac ggttgggttt ttaataaagc ctgaatcagg
    15241 ctggatttac ccgcgccact gggggcagaa acaatataaa gcgtgccttg agccatgagt
    15301 atctttcgta tgtgattagc gaaataaagc ctacatacga gcttattata cacggcgcgg
    15361 ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt tgttgcgttt tgcgtagctg
    15421 cttccgcatt tgcctgtgat ttactgcaat aaagacgatt ctcaggaagc gtcctcgcag
    15481 actccgtttt tgccactcgg ctttgccttc accacgttgt atatctgcca gcctgactac
    15541 agcggaggtg aagatgaaag tatggatggc gatattaata agtatcttgt gctggcaatc
    15601 atctgtgtgg gcggtctgtc cggcctggtc gccagccaga gcacaggaag aaattttccg
    15661 ccagcaacag caaataaaac agtgggacga tgactactgg aaggaaggaa aaagtgaggt
    15721 ggaagacggt gtttacgatc agttaagcgc ccgtcttacg cagtggcaac gctgctttgt
    15781 gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt gcggttatgc atccggttgc
    15841 ccatacgggt gtacgcaaaa tggcggataa aaacgcatta agtctgtgga tgcgagaacg
    15901 tagcgatctt tgggtgcagc caaaagttga tggcgttgcg gtaaccctgg tttatcggga
    15961 cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg aaaggcgagg actggacgca
    16021 gaaagttagc ttaatttccg ctgtgccgca aaccgttagc gggcctttag ccaacagtac
    16081 gcttcagggg gaaatatttc tccagcgcga ggggcatatc caacaacaaa tggggggaat
    16141 aaatgcccgc gcaaaagttg ctggcttgat gatgcgccag gacgatagcg acacgctgaa
    16201 ttctctgggc gtttttgtct gggcatggcc ggatggaccg cagttaatgt ctgatcgttt
    16261 aaaagagctg gctaccgcag gttttactct aacgcagacg tatacccgtg cggtgaaaaa
    16321 tgctgatgaa gttgcgcgcg tacgcaatga gtggtggaaa gcggaattac ccttcgtcac
    16381 cgatggcgta gttgtacgag cggcgaaaga gccagaatcc cgccattggc taccgggcca
    16441 ggcagagtgg ctggtggcct ggaaatatca acctgtagct caggttgccg aagtgaaggc
    16501 gattcagttt gcggtgggta agagcggtaa aatatcggtg gttgcgtcac tcgcatctgt
    16561 catgctggat gataaaaaag tccagcgggt gaatattggt tccgtcaggc gctggcagga
    16621 gtgggatatt gcgcctggtg atcagattct cgtcagcctt gccggtcagg gtattcctcg
    16681 cattgatgat gtggtgtggc gcggtgcaga acgtacaaaa ccgacaccgc cagaaaaccg
    16741 ctttaactcg ttgacctgct actttgcttc tgatgtttgt caggaacagt tcatttcacg
    16801 cttagtctg
//
LOCUS       DAJVTY010000088        16652 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVTY010000088 DAJVTY010000000
VERSION     DAJVTY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16652
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.088"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..181)
                     /locus_tag="OQQ66_002502"
     CDS             complement(<1..181)
                     /locus_tag="OQQ66_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055794.1"
                     /note="catalyzes the of heptose(II) of the outer membrane
                     lipopolysaccharide core; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1905390.1"
                     /translation="MSLNFTVIVWLRLSIGGVYELAFCRSSLKSKKYFIILLALAAIA
                     GLGTHAAWSSNGLPRI"
     gene            432..1571
                     /gene="arnB"
                     /locus_tag="OQQ66_002503"
     CDS             432..1571
                     /gene="arnB"
                     /locus_tag="OQQ66_002503"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1905391.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1575..2543
                     /gene="arnC"
                     /locus_tag="OQQ66_002504"
     CDS             1575..2543
                     /gene="arnC"
                     /locus_tag="OQQ66_002504"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1905392.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2543..4525
                     /gene="arnA"
                     /locus_tag="OQQ66_002505"
     CDS             2543..4525
                     /gene="arnA"
                     /locus_tag="OQQ66_002505"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1905393.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4522..5412
                     /locus_tag="OQQ66_002506"
     CDS             4522..5412
                     /locus_tag="OQQ66_002506"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1905394.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5412..7064
                     /gene="arnT"
                     /locus_tag="OQQ66_002507"
     CDS             5412..7064
                     /gene="arnT"
                     /locus_tag="OQQ66_002507"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1905395.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7061..7396
                     /gene="arnE"
                     /locus_tag="OQQ66_002508"
     CDS             7061..7396
                     /gene="arnE"
                     /locus_tag="OQQ66_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1905396.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7396..7782
                     /gene="arnF"
                     /locus_tag="OQQ66_002509"
     CDS             7396..7782
                     /gene="arnF"
                     /locus_tag="OQQ66_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1905397.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7776..8042)
                     /gene="pmrD"
                     /locus_tag="OQQ66_002510"
     CDS             complement(7776..8042)
                     /gene="pmrD"
                     /locus_tag="OQQ66_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1905398.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8152..9507)
                     /gene="menE"
                     /locus_tag="OQQ66_002511"
     CDS             complement(8152..9507)
                     /gene="menE"
                     /locus_tag="OQQ66_002511"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1905399.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9504..10466)
                     /gene="menC"
                     /locus_tag="OQQ66_002512"
     CDS             complement(9504..10466)
                     /gene="menC"
                     /locus_tag="OQQ66_002512"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1905400.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSIAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10466..11323)
                     /gene="menB"
                     /locus_tag="OQQ66_002513"
     CDS             complement(10466..11323)
                     /gene="menB"
                     /locus_tag="OQQ66_002513"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1905401.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11338..12096)
                     /gene="menH"
                     /locus_tag="OQQ66_002514"
     CDS             complement(11338..12096)
                     /gene="menH"
                     /locus_tag="OQQ66_002514"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1905402.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLEGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(12093..13763)
                     /gene="menD"
                     /locus_tag="OQQ66_002515"
     CDS             complement(12093..13763)
                     /gene="menD"
                     /locus_tag="OQQ66_002515"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1905403.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13852..15147)
                     /gene="menF"
                     /locus_tag="OQQ66_002516"
     CDS             complement(13852..15147)
                     /gene="menF"
                     /locus_tag="OQQ66_002516"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1905404.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15226..15531)
                     /gene="elaB"
                     /locus_tag="OQQ66_002517"
     CDS             complement(15226..15531)
                     /gene="elaB"
                     /locus_tag="OQQ66_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1905405.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15586..16047)
                     /locus_tag="OQQ66_002518"
     CDS             complement(15586..16047)
                     /locus_tag="OQQ66_002518"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1905406.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            16112..>16652
                     /locus_tag="OQQ66_002519"
     CDS             16112..>16652
                     /locus_tag="OQQ66_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCS1905407.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKA"
BASE COUNT         3888 a         4493 c         4241 g         4030 t
ORIGIN      
        1 cgatacgtgg caaaccattg ctactccagg cggcatgagt acccagtccg gcaattgcag
       61 cgagcgccag taaaatgatg aaatattttt ttgacttcaa tgatgagcgg caaaaagcta
      121 actcgtaaac tccacctata gacaagcgca accagacaat taccgtgaaa ttgagcgaca
      181 tttctggcga taattagcag ttggtgtaat attaaaaatc ctacgatgtc ggcaaaatgc
      241 ctcaaaattt tgccaaatgc aaagcctaaa taagaaaaaa tataaaaatt tcaatattta
      301 cgtctaatat tagtttctta aggttaagtt aatattctat ccttaaaatt tcgttccaaa
      361 tggcaaaata tacacaacac tctttatagc aaatataagt ggacaggtat tcaatggcgg
      421 aaggaaaagc aatgtcagaa tttttgcctt tttcgcgacc agcaatgggc gtggaggaac
      481 tcgctgcagt taaagaagtt ctcgaatccg gttggatcac aaccggtccg aagaatcagg
      541 cgcttgagca agccttttgc cagttgacgg gaaatcagca tgccatcgcg gtcagttcag
      601 ccaccgccgg aatgcatatc acgctaatgg cgttggaaat tggcaagggc gatgaagtga
      661 ttacgccttc cctgacctgg gtttcaaccc tcaatatgat ttccttgttg ggtgcaacgc
      721 cggtaatggt ggatgtcgac cgcgatacgc tgatggtcac gcctgaagcg atcgagtcag
      781 ccattacgcc acgcactaaa gccatcattc cggtgcatta tgccggtgcg ccagcagata
      841 ttgacgccat tcgcgccatt ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc
      901 atgccgtcgg tacgtattac aaagggcgac atattggcgc aaaaggtacc gctatttttt
      961 catttcatgc cattaaaaat attacctgtg cggaaggtgg cctgattgta actgataatg
     1021 aaaaccttgc ccgccagcta cggatgctga aatttcacgg cctgggtgtc gatgcctatg
     1081 acagacaaac ctggggccga gcaccgcagg ctgaagtctt aacaccgggc tataagtaca
     1141 atctgaccga tattaacgcc gcaattgccc tgacacagtt agcaaaatta gagcacctca
     1201 acacccgtcg gcgcgaaatt gcccagcaat atcagcaagc actggctgct ctcccctttc
     1261 agccattaag ccttcccgcc tggccgcacg ttcacgcctg gcatctgttt attattcgtg
     1321 tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag
     1381 gcattggtac cgggttacat ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt
     1441 ttcccacgct gtcgttaccg aataccgaat ggaatagcga acgcatctgt tctttgccgc
     1501 tgttcccgga tatgactacc gccgatgccg accgtgtcat cacagccctt cagcaactcg
     1561 caggacaata agccatgttt gaaatccacc ctgttaagaa agtctcggtg gttattcccg
     1621 tttataacga gcaggaaagc ttaccggaat taatcaggcg caccaccaca gcctgtgaat
     1681 cgttggggaa agagtatgag atcctgctga ttgatgacgg cagtagcgat aattccgcgc
     1741 atatgctggt cgaagcctca caagcggaga acagccatat tgtgtctatt ttgcttaacc
     1801 gcaattacgg gcaacattca gcgattatgg cgggattcag tcacgttact ggcgacttaa
     1861 ttattaccct tgatgccgat ctccagaatc cgccagaaga aatcccccgc ctagtggcaa
     1921 aagccgatga aggttacgac gtggtaggga ctgtacgcca gaaccgccag gacagctggt
     1981 ttcgtaaaac agcttcgaag atgattaacc ggcttattca gcgcaccacc ggcaaagcga
     2041 tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc
     2101 actgccatga acgtagcacc tttatcccga ttctggcgaa tatcttcgcc cgccgtgcca
     2161 ttgaaattcc agtacatcat gccgagcgtg agtttggtga atccaaatac agttttatgc
     2221 gcctgattaa tttgatgtac gacctggtga cctgccttac cacaacgccg ctacgtatgc
     2281 tgagtctgct cggcagcatt attgcgattg gaggttttag cattgcggtg ctgctggtga
     2341 ttttacgcct gaccttcgga ccacaatggg cggcagaagg cgtctttatg ctatttgccg
     2401 tgctgtttac ttttattggc gctcagttta tcggcatggg attactcggt gaatatatcg
     2461 gcaggatcta caccgatgtc cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc
     2521 catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt gcctaccacg atatgggatg
     2581 cctcggtatt gaagctctgc tggctgccgg ttacgaaatt agcgccattt ttacccatac
     2641 cgataatccc ggtgaaaaag ccttttatgg ttcggtggct catctggcgg cggaaagagg
     2701 cattccggtt tatgcgccgg ataacgttaa tcatccgctg tgggtggaac gcattgccca
     2761 actgtcacca gaggtgattt tctcttttta ttatcgccat cttatttgcg acgagatttt
     2821 gcagctcgct cccgcaggtg catttaatct gcacggttcg ctgttaccaa aatatcgtgg
     2881 tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaacg gaaacgggcg ttacattgca
     2941 ccgaatggta aaacgtgccg atgctggggc cattgtggcg caactgcgcg ttgccattgc
     3001 gccagacgat atcgctatta cgctgcatca taaattgtgc catgccacgc gccagctact
     3061 ggaacagaca ttacccgcca ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa
     3121 cgaagccacc tgttttggtc gcagaacgcc ggatgacagt ttccttgaat ggcataaacc
     3181 ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag
     3241 ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa
     3301 agcacagccg gggagcgtga tttctattgc accgctgttg attgcctgtg gcgatggcgc
     3361 gctggaaatc gtcaccggac aggcgggcga cggcattact atgcagggct cgcaattagc
     3421 gcagacgctg ggcctggtgc aaggttcacg cttgaatagc cagcctgcct gcaccgcccg
     3481 acgccgtacc cgggtactca tcctcggggt gaatggcttt attggcaacc atctgacaga
     3541 acgcctgctg cgcgaagatc attatgaagt ttacggtctg gatattggca gcgatgcgat
     3601 aagccgtttt ctgaatcatc cgcattttca ctttgttgaa ggcgatatca gtattcattc
     3661 cgaatggatt gagtatcatg tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc
     3721 cacgccgatt gaatataccc gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa
     3781 tctgcgcatt atccgctact gcgtgaagta ccgtaagcga atcatcttcc cgtcaacttc
     3841 agaagtttat gggatgtgta gcgataaata cttcgatgag gaccattcta atttaatcgt
     3901 cggcccggtg aataaaccac gctggattta ttcggtatca aaacaattac ttgatcgggt
     3961 gatctgggcc tatggcgaaa aagagggttt acagttcacc ctcttccgcc cgtttaactg
     4021 gatgggacca cgactggata accttaatgc ggcacgaatc ggcagctccc gcgctattac
     4081 gcaactcatt ctcaatctgg tagaaggttc accgattaag ctgattgatg gcggaaaaca
     4141 aaaacgctgc tttactgata ttcgcgatgg tatcgaggcg ttataccgca ttatcgaaaa
     4201 tgcgggaaat cgctgcgacg gtgaaattat caacattggc aatcctgaga acgaagcgag
     4261 cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa aaacatccgc tgcgccatca
     4321 tttcccaccg tttgcgggct ttcgcgttgt cgaaagtagc agctactacg gcaaaggata
     4381 tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc caccgctgcc tggactggga
     4441 gccgaaaatt gatatgcagg aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt
     4501 tgatcttacg gataaaccat catgaccaaa gtaggcttac gcattgatgt cgataccttt
     4561 cgtggcaccc gtgaaggcgt gccgcgtctg ctggaaatct tgagtaagca taatattcag
     4621 gccagcattt ttttcagcgt cggcccggac aatatgggcc gccatctctg gcgactggtg
     4681 aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg catcgcttta tggctgggat
     4741 attttactgg caggtacggc ctggccaggt aaagagattg gtcatgccaa tgccgatatc
     4801 attcgtgaag cggctaaaca tcacgaagtc ggcctgcacg cctgggatca ccatgcctgg
     4861 caagcccgta gcggtaactg ggatcggcaa acaatgatcg acgatattgc acgcggtctt
     4921 cgcactctgg aagagattat cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt
     4981 gccgaccaga aggtgatcga agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt
     5041 cgtggggcca tgccgttccg tccattgctc gaatcaggaa accctggcac tgcgcaaatt
     5101 ccggtgacct tacccacctg ggatgaagtg attggtcggg atgtgaaaac agaagatttt
     5161 aacggttggt tactcaaccg catcctgcga gataaaggca cgccggttta taccattcat
     5221 gcagaagttg aaggctgcgc ttatcagcat aattttgtgg atctcctcaa acgcgcagct
     5281 caggaaggcg tgacattttg ccctttaagc gaactgttat cagagacgtt gccgctcgga
     5341 caagttgttc gcggaaatat tgccggacgt gaaggctggc tgggttgcca acaaattgcg
     5401 ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct cttcgcgttt attgcctgct
     5461 attacctgtt accgatcagc acgcgtctgc tctggcaacc cgatgaaacg cgttatgcgg
     5521 aaatcagtcg agaaatgctg gcatccggcg actggattgt gccccatctg ttagggctac
     5581 gttatttcga aaaacccatt gccggatact ggattaacag cattgggcaa tggctatttg
     5641 gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc gaccctgtta actgccgcgc
     5701 tggtgacctg gtttactctg cgcttatggc gcgataaacg tctggctcta ctcgccacag
     5761 taatttatct ctcattgttt attgtctatg ccatcggcac ttatgccgtg ctcgatccgt
     5821 ttattgcatt ctggctggtg gcgggaatgt gcagcttctg gctggcaatg caggcacaga
     5881 cgtggaaagg caaaagcgca ggatttttac tgctgggaat cacctgcggc atgggggtga
     5941 tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag cgtgctgcca tgggtagcga
     6001 cgcaaaaacg ctggaaagat ctctttattt acggctggct ggcggttatc agttgcgtac
     6061 tgacggttct cccttgggga ctggcgatag cgcagcggga gcctgacttc tggcactatt
     6121 ttttctgggt tgagcatatt caacgctttg cactggatga tgcccaacat agagctccgt
     6181 tctggtacta cgtgccggtc atcattgccg gtagcctgcc gtggctggga ttactccccg
     6241 gtgcactgta cacaggctgg aaaaaccgca agcattccgc aaccgtctat ttgttgagct
     6301 ggacgataat gccgctgctg tttttctccg tcgctaaagg taaattgccc acctatattc
     6361 tttcctgctt tgcatctctg gcaatgctga tggcgcatta cgctttgctg gcagcaaaaa
     6421 ataatcctct ggcgctgcgg attaatggct ggattaacat cgcttttggc gtcactggca
     6481 ttattgccac atttgtggtc tccccgtggg gaccaatgaa cacgccggtg tggcaaacct
     6541 tcgagagcta taaagtcttt tgtgcctggt cgattttttc gctatgggca tttttcggct
     6601 ggtacacctt aacaaacgtc gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc
     6661 tggcgttgct ggtaggattt tcaattcctg acagagtcat ggaaggaaaa catccgcaat
     6721 tttttgtcga gatgacacaa gaatcacttc aaccaagccg ctatattctt actgatagcg
     6781 tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc
     6841 agacaggtga gttgaaatac ggccttaatt atccggatgc gaaagggaga tttgtcagcg
     6901 gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga ggggattatt acactcgtgc
     6961 tttcggttga ccgcgatgaa gatatcaaca gtctcgccat tccgcccgca gatgccatcg
     7021 atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa atgatctggc taacattagt
     7081 ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt
     7141 ggcgataaac aaacggcgca aacatatcgt gctgtggctg ggactggcgc tggcttgtct
     7201 tggtcttgcc atggtgctct ggctgctggt cttgcagaac gtaccggtag gcattgccta
     7261 cccgatgtta agtctgaatt ttgtctgggt gacgctggct gcagtaaaac tgtggcacga
     7321 accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt attggtggca ttgtgatcct
     7381 cgggagtacg gtgtaatggg cctgatatgg ggattattca gcgtcattat tgcctcagtt
     7441 gcgcaattaa gcctgggttt tgcggcaagt catctgccgc cgatgacgca cttatgggat
     7501 tttatcgcgg ctctgttagc ttttggcctg gatgcccgga tactgttact cgggctgctg
     7561 ggttatctgc tttccgtttt ttgttggtat aaaacgctac ataaacttgc gctaagcaaa
     7621 gcctatgcct tgctcagtat gagttatgtg ctggtatgga ttgcttcaat ggtacttccc
     7681 ggctgggaag ggactttttc gctgaaagca ctactgggag tagcctgtat tatgagcggg
     7741 ttgatgctga ttttcctgcc cacgacaaaa caacgttact gagttttccc tgccgctgta
     7801 cactgccgtt cccactcatc ggggctataa ctgcttgccg agaggacttt caccgtatgc
     7861 agcttttcac gattaataca ataaagcgca ttctgcaaag gcgagagtaa atcccccact
     7921 ttcacggcga agtcactctt cacttcggcg atcattttta tcgcgccgcc agcatcgcag
     7981 agcataagta catgtctgtt atcttgttta ttgcagcacg attttttgac cagccattcc
     8041 attgcattat cctgtttgct aagagttttc acatcaattt acagattaac agataaactc
     8101 tttatcaacg gcaatggaat ggcagaaatg agaagtgaaa cggagggtta attattgctg
     8161 acgttgcacc cactctttta gcgcctgacg tgaaatttta ataccgccgt ttttcagctc
     8221 cggcggtaaa gtcagccagc gcaccggttg ttgaaaacga gccagcttat ctttcaccca
     8281 ttcgccaaga tcaacggttt gctggtcata ctccaccacc gctaccggcc gatgaccaaa
     8341 ctccttgtcg gcaacgggaa cgataaacac ctgcaaaacc gcaggatgtg cagcaataac
     8401 acgctcgact tcttccggct gaataccctc accgccactg aataacagat tgtctaaacg
     8461 tccgacaatg gtcagcttgc cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc
     8521 ttcgtcatta accagtgaaa ccagttgccc gttacgccag taaccttctg ccatactggc
     8581 agcccgcagc cacacttcat tattaacgat tttcacttcc cgacccggca gcggcgaacc
     8641 aacgtctgcc aggccgtcgg cttctttcgc acataccgtg gaggcaaact cggtcagacc
     8701 atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat
     8761 agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg
     8821 ccagagttgt gttggcacca gtgaagcgtg agtacagcct gccagcattt gctccaatgg
     8881 ctgtttatca cgcaccgtca tccgcgcacc agcgtataac cagcgccaca taattccctg
     8941 accggagacg tgaaacagcg gtaaagagag caaccagtca tcgtgatcgc caaacggaat
     9001 cagcgataac acaccttgcg cactggcaag atgggcctga taagtatgga cagcggcttt
     9061 cggtaaaccg gtggagccgg aggtcaatgt cattgaacaa agacgtgttg gttgccacag
     9121 agctgaatgt gtgccttcaa ccatctgaat atgcagtgaa gttaacgccg ggaaagtatt
     9181 ttctccttcc agcaccagcg catattgcag tgtaagattt ggcagtaatt cctcaagcaa
     9241 cggttgcgga agctgtggat ttacgggcag cacccgcgcc ccgcattgca gtaacgccag
     9301 ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac atcacgccgc tgccctcaac
     9361 cacaccctgc gccgcaaagc ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca
     9421 gttgagttgc tcgtcattaa gacgtaaggc gatggcttct ccccgcactt gccgccagtg
     9481 acgccacggc cagtcagaga agatcataac aaccgctcca gtgcatcaac ttccacgaca
     9541 ggcagcgtgc tacccggcca gcgacgtacc tgctgcgcct gcatcagatc cagcgtgtcc
     9601 agccctggaa tggtgtccgg cgttaaccag gcggcaatcc gcgccagttg cgttaagcct
     9661 aagctcgact caatagaaga actgatcacc gccgtcagcc tcaacgcgtg cgccgcctgt
     9721 acctgctcgc gtactttttc cagactgccc gtgagcgtgg gtttgataac taccgcgcgc
     9781 acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg
     9841 gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc gggttttgca cggctcttcg
     9901 agaaacgcga tgcggtggcg ataatccggg ttaacatatt tggcaaactg ctgacctttc
     9961 agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc
    10021 aacagattaa ccaccatgcc gtcgcgcacg gcttcgtaca atcccacttt gaccttcgcc
    10081 actttctcgc ctggcatatc agcaagtttg aggatcagat cgtcaggatc gccattacac
    10141 agtggtgccg cacggtaatt ggccgcctgc gggagcgtat ctgccagctc cgccaatgca
    10201 cagcttacgc caaaggcaat ggaaggcatc tgcagtagct cgcaatcgcc tgccagccag
    10261 ttatttaccc aggcaagcag cacgctttgc gcctcttccc aggtttcctg gctgaagccc
    10321 ggcagtgggg agatctcccc ccacccttcg cgctcgccct cacgcaggca aacgtacagc
    10381 ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc ccgcgtccat ggggatctgc
    10441 cagcggtata cctgcgcgct acgcattacg gattccgttt aaatttgctg aaatcaggct
    10501 gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc ttccgtcatg tagaacagca
    10561 tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc gtcacagtcg gcgttcagtg
    10621 cagctttcag gcagcgcagc gccatcggac tgttttgcag catttcgcgg caccagcgaa
    10681 cggtctcttt ttccagatcc gccagcggta ccacggtgtt gacaaggccc atatccagcg
    10741 cctgtttcgc gtcgtactga cggcacagga accagatttc ccgcgctttt ttctgtccga
    10801 cgatgcgagc catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag
    10861 tctgaccgaa gatggcatta tctgccgcga tagtcaggtc gcacatcatg tgcagaacgt
    10921 gaccgccgcc gatggagtag ccagccacca tcgcgacaac cggtttcgga caggtacgga
    10981 tctgacgctg gaagtccagc acgttcaggt gatgtacgcc ggaatcatct ttatagccgc
    11041 cgtaatcacc acgcactttc tggtcaccgc cggagcagaa cgctttatca cctgcgccag
    11101 tcagaatgat cacgccgacg ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct
    11161 cttttaccgt cagaggacgg aaggcattgc gcacctgcgg acgattaatg gtgatttttg
    11221 cgataccatc ggtggatttt tcataacgaa tgtcctcgaa accttcggag cagtcgtgcc
    11281 attcaaccgg tgcgtaaagc attgcttcat caggataaat catattgtgt cctttagtca
    11341 gaaacgcaag atctgcgcca gacttgcgat tacgccagcg ggattttccc gatgcgcgtt
    11401 atgtccggcg cgaggaatga catggcagtc ggcagccagt tccgccgcca gggcgcggaa
    11461 tttgctgtca cgttcaccac ataaataata aaacgcaaat gtgcgggcgc taaggttagc
    11521 acgtaaatca ggctggacgg cgagagaagt cgcctccagc atggcggcaa gggttgcgcc
    11581 attattgttg ctgcgcagcg ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa
    11641 aacaggctgt tgataccagt cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg
    11701 cgcccattgg cgatcggaac gctgacgttc cgcacgttgt tcagcatttt gcagccccgg
    11761 atgcccgcct tcgacaataa ccccacaaag ccccgccagc ccctggcaag ccgccatcat
    11821 cgccacccgt ccgccaagcg agtacccctc cagccagaag tcaaggatgt tgtaactaac
    11881 caaggtttta cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa tcgccgccga
    11941 accaccatga cctgggagat caacatacaa ccgtgagtag tcggcaaacg cctcgcccac
    12001 ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc
    12061 tggttttccg tgttttgcct gcgcgtgcag gatcataaat ggcttacctg cgccagaagt
    12121 tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca tttcaatcac cgtggtggtt
    12181 ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct gccagttttg cggacgatga
    12241 tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag
    12301 aaacgctcac gctcgctttg cggcgttggc aacagcgaga aaatttgccc gccgttgttg
    12361 ttcaccacaa ttaataccag cggcgcagaa acctgacgca ataacgccag cgcgttgaga
    12421 tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg gtttgccgct tgcccgctga
    12481 acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg cccctcggtt gctgtacacc
    12541 gggtaacctg ccggaagttg cgaaagcgca tcaatcagac gtaccaccag gctgttaccg
    12601 acaaacaatt gcccctgttc aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg
    12661 ccaaacgcat cgcgacgggc aataaccgcc tgcattgcct gttccgccag gcgcgggatt
    12721 tcaacgcacc agggctggcg tttttctgcc ggatgcagct ccagccagtc ggcaatattg
    12781 gcaattaagc ggcgtccacg atggtgcgcc ggatcaagcc gcccttcgat gtcatccaca
    12841 atccagtact cttcgggttc acagcttgcc tgccattgca ggagccgttt gcccgtcagg
    12901 ctgcttccca gttgcaccac aatttgcgcc tgctgcagct cgctggtcgc tttggcattg
    12961 cctaaccaaa gatcggcaca cggcagcggc tgcccggttt gtgacagcac atcgccaatc
    13021 agcggccagc caagagtttg cgcccacagg gcaacttttt tgccctcttc cgcactcatg
    13081 cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga accagtcgcg ctgtttttca
    13141 ctttccagac taggcgcttc acgcagccac ggtttgtcgt cctgccacca gtcacccaga
    13201 cgctgttgcc agctaagccc ggtatcgtcc atttcgccat acagcggttc agcaaacggg
    13261 cagttgatat ggactccccc cgcatgaagc gtaccgagag cgtggtcgat ggtagaaacc
    13321 agccaacgtg cggggatatc ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga
    13381 gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc cgcagtcaat tagctccggc
    13441 gggcgatcgg cggttaagag aatcagtttt tctccggtta acccggcttc aatcagtgcc
    13501 ggatagagat ttgccaccgc agtgccggag gtcacaatca ccgccaccgg ctgcttgctg
    13561 actttcgcca gccccagcgc cagatgcccc aacccacgct catcgaaatg ggtgtggtga
    13621 atgaatgcgg aattctccgc cgccgctaac gttaacggtg tagaacgcga gcctggggcg
    13681 atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt
    13741 cggttaaatg cgcttactga catgagtttg tccggtatca atattgcggc taagtataag
    13801 gagctacaaa aatagattat tgatatgaat cggtaatgat gcgactcatt actattccat
    13861 ttgtaataaa gtacgcaacc ctgccgcttt gttgtcgatt tcctgccact cttgctcggg
    13921 gtcggaacca cggacaatgc ccgcgccagc atataatcgc acgacattgc cgctaatttt
    13981 tgctgagcgc agggaaacgc agaattcgct ttgttgtaat gagagatagc ccgcagaacc
    14041 ggcgtaccat tctcgggtga acggttcgtg acgggcgata aactgtcgcg ccagatcgcg
    14101 cggtaagcca gcaactgctg ccgttggctg caactgatgt aaacagatca catcatccgc
    14161 tttgttgagt gaagtccaga tacagcggcg aagatgctgc actttacgca gacgcagtac
    14221 ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt aatcgttggc agatatcttc
    14281 caccaccagc atgttctcgc gctggttttt atcatccgcc atcagccact cttctaactg
    14341 ctgcgcctgc ttatcatcag gatgatttgc tactgttccc gccagcgctt cagtacgcag
    14401 cgctttgtca cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag cattttcgcc
    14461 atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt cgacgactgg cagccatcat
    14521 cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt
    14581 gtcgagctca ccttcggcga tggttttcgt tgccagttcg attaattgcg tccagcccgt
    14641 tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt aacccaggca agggcttgat
    14701 actcaccagt gtggcgataa attcttttgc ctgaatcgca tcgtgctgaa gggagctttc
    14761 gctgaatagc gtcagccgca gcgtggcttt accgccacag cgtcgccatt ccaggcgggg
    14821 taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc ccccaaatgc gtaagtcggc
    14881 gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat
    14941 cgcgcccagg acggcagctt cttcatcacc attacgttgt tgccagtaga attgcgggta
    15001 cgtttgctga ctggccagcc agctcaaggc atcaaaagcg tctttgagag ggaaaggaat
    15061 atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt
    15121 ttccagcgcc gtagtaagtg attgcacgcg gacctctccc tgttaaaagc ctcacattat
    15181 acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag
    15241 caacagtcct agtaccagcc caacggccgc gcccacacca attccttgcc aaggtttttc
    15301 gtggacgtag tcatcagcac gataaacagc ctgcttcgcc cgatagtaat aactgtctga
    15361 aacctggcta acccgttttt tcacatcatc cagcgctttt tctgcacgcg ctttcagctc
    15421 aacatatttc tgatcagcgg gatcgccaga ggagcggagc acctcttcca gtgtctcact
    15481 aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac tgattagaca ttctcgttct
    15541 ccatttgcgt aaaacctgtc actaactata gacaagggtt gttgattacg cctgaattac
    15601 ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca tagacctccg tcacagggat
    15661 aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc gccccgaggt aaacaggctt
    15721 atcaggccag tgatgcgtgc aggtgtcgag ggttttactc atcagttgct gccccacttt
    15781 ttcgccacgc aacgcttcac tgacaatcac ccgaccaata acgaccggct caagatcatc
    15841 atcacttttc agaatcctcg catatgccac cagttcatca tttttccaac cgagaatatg
    15901 gcgattatcc cccgtcaaat catcaccatc gatatcctga taagggcaat tctgctcgac
    15961 aacaaacacc gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg ataattcaga
    16021 gtgatgcaga tcttgccatt caatcataat gctctccttt tagatgtata ccttatacta
    16081 gccggaatat tttttgaaac gtgatgaact catggaatta atttttttag gtacttcagc
    16141 cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg cttaacctgc aacatccgac
    16201 ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc tactgcatac
    16261 cgcctttaac cctggaaaac tggacaagat ttttatcagt caccttcatg gcgatcatct
    16321 ttttggttta cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc aacccttaac
    16381 gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc gcgctgcgga ttagcggctc
    16441 atggaccgat tatccgctgg aaattgtcga aattggcgct ggcgaaattc tcgatgatgg
    16501 cctgcgcaaa gtaaccgctt atccgctgga acacccactg gaatgttatg gctatcgtat
    16561 tgaagaacat gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc
    16621 gcctggccca ctgtttcagg aattaaaagc gg
//
LOCUS       DAJVTY010000089        16648 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVTY010000089 DAJVTY010000000
VERSION     DAJVTY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16648
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.089"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..643)
                     /locus_tag="OQQ66_002520"
     CDS             complement(<1..643)
                     /locus_tag="OQQ66_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1905408.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALT"
     gene            complement(780..1232)
                     /gene="moaE"
                     /locus_tag="OQQ66_002521"
     CDS             complement(780..1232)
                     /gene="moaE"
                     /locus_tag="OQQ66_002521"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1905409.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1234..1479)
                     /gene="moaD"
                     /locus_tag="OQQ66_002522"
     CDS             complement(1234..1479)
                     /gene="moaD"
                     /locus_tag="OQQ66_002522"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1905410.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1472..1957)
                     /gene="moaC"
                     /locus_tag="OQQ66_002523"
     CDS             complement(1472..1957)
                     /gene="moaC"
                     /locus_tag="OQQ66_002523"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1905411.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(1960..2472)
                     /gene="moaB"
                     /locus_tag="OQQ66_002524"
     CDS             complement(1960..2472)
                     /gene="moaB"
                     /locus_tag="OQQ66_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1905412.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2494..3483)
                     /gene="moaA"
                     /locus_tag="OQQ66_002525"
     CDS             complement(2494..3483)
                     /gene="moaA"
                     /locus_tag="OQQ66_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1905413.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3471..3615)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3880..4788
                     /gene="yvcK"
                     /locus_tag="OQQ66_002526"
     CDS             3880..4788
                     /gene="yvcK"
                     /locus_tag="OQQ66_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1905414.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(4980..7001)
                     /gene="uvrB"
                     /locus_tag="OQQ66_002527"
     CDS             complement(4980..7001)
                     /gene="uvrB"
                     /locus_tag="OQQ66_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1905415.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVETQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7580..8257)
                     /gene="bioD"
                     /locus_tag="OQQ66_002528"
     CDS             complement(7580..8257)
                     /gene="bioD"
                     /locus_tag="OQQ66_002528"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1905416.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8250..9005)
                     /gene="bioC"
                     /locus_tag="OQQ66_002529"
     CDS             complement(8250..9005)
                     /gene="bioC"
                     /locus_tag="OQQ66_002529"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1905417.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(8992..10146)
                     /gene="bioF"
                     /locus_tag="OQQ66_002530"
     CDS             complement(8992..10146)
                     /gene="bioF"
                     /locus_tag="OQQ66_002530"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1905418.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10143..11183)
                     /gene="bioB"
                     /locus_tag="OQQ66_002531"
     CDS             complement(10143..11183)
                     /gene="bioB"
                     /locus_tag="OQQ66_002531"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1905419.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11270..12559
                     /gene="bioA"
                     /locus_tag="OQQ66_002532"
     CDS             11270..12559
                     /gene="bioA"
                     /locus_tag="OQQ66_002532"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1905420.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12618..13094
                     /locus_tag="OQQ66_002533"
     CDS             12618..13094
                     /locus_tag="OQQ66_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1905421.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13246..14529
                     /locus_tag="OQQ66_002534"
     CDS             13246..14529
                     /locus_tag="OQQ66_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1905422.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14670..>16648)
                     /locus_tag="OQQ66_002535"
     CDS             complement(14670..>16648)
                     /locus_tag="OQQ66_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1905423.1"
                     /translation="LCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAIIHQYMREMMA
                     GGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPY
                     VGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQ
                     TDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDT
                     LNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAA
                     ANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGD
                     TPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDC
                     WDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKI
                     LDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVS
                     ELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAE
                     EYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDA
                     PVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         3829 a         4567 c         4272 g         3980 t
ORIGIN      
        1 aggttaacgc gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct
       61 gctcacccat atttttcaat ttctgagtgt catacgccgt caatccgaca aagacaatca
      121 cgccgatgta ggtaactgcc cacatcaatg cttcgctttt cagccagaag ttgaccagcg
      181 atgccagcac aatgccgatt aacgccataa acagcatatt gccaaagcca cttaaatcgc
      241 gcttcgtggt gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag
      301 tactggcgat agaagcagcg gtatagacaa tgaatatact ggaaagcgta agacccgtca
      361 gcgccgaata aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg
      421 ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt
      481 tagtgaacaa cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg
      541 tcagcaacaa gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag
      601 cccggggttg tacgattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat
      661 ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac
      721 gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaaac
      781 taccagcgtt ttgccgcctg ctgatcgctc tcccgagctt caacccagcg gtcgccttcc
      841 ggcgtggctt cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc
      901 ccggcttcaa acgcactgct gcgatgcgca ctggtgacac cggcaaaaac gatttcatcg
      961 cccggccata attccccgat gcggtgaatc acagtgacgc gccccagcgg ccagcggtta
     1021 cgcgcttcat caacaatttc tgccagtgct ttttcagtca ttcccggata gtgttcgagg
     1081 gttaatgctt tgacgctgtc gcccaggtta tggttgcgca ccttaccagt aaaggtgact
     1141 accgcaccgt cttcgtcacg ctccgccagc cacgggtact cttctcctac gctgaacggc
     1201 tgcggaccaa caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa
     1261 agctacttcg tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc
     1321 agccagtaat ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccatgtg
     1381 ctggcgtaac gcttcaacag ttgggaaatc cgcagccact tcggttgcat ctgttcccac
     1441 caactcgcgc acctgcgcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac
     1501 ccgacttgcc gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca
     1561 ccgctttgca catgtcataa atggtcagcg ccgccacgga ggccgcggtt aatgcttcca
     1621 tttcgacacc ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct
     1681 ccggctcggc ctgtaaattg acttcaactt tgctgagcat cagcggatga cagagcggga
     1741 tcagatccca ggtgcgtttt gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt
     1801 cgcctttgtg gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa
     1861 aggcttccgc ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg
     1921 cttcgccagc ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg
     1981 gaaattacac ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt
     2041 acggcacgct ttggtcgaac ccggcatggc gaaaatcagc gttttgttgg cgacgcccgc
     2101 aaccgcacga gattgcaacg tggaagtgcc aatctcttca aacgacaaca tccggaacac
     2161 ttcaccaaaa ccttcaactt cacggtcgaa caacggcagc aatgcttcgg gagcctgatc
     2221 accttccgtc aggccagtac cacccgtaat caataccact tgtacatcgt cgctggcgat
     2281 ccacgcagat acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac
     2341 aacgtgatgg cccgcttctt gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc
     2401 ttcaccgcga cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct
     2461 tacctgactc atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg
     2521 cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc
     2581 tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt
     2641 aacgccgcct tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg
     2701 gttgcaagtg gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta
     2761 atctggatgg caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca
     2821 gccgcgacgc agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag
     2881 ctcgctgccc tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg
     2941 gatccagttc agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt
     3001 gactttgacc ttctcaaaac cggcctcaaa tgcagcatca atccctgcca tgacctggtt
     3061 gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt cgacactgac
     3121 gttaatgcca gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca gacggtaacc
     3181 attggtggtg accgcaatct ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc
     3241 ggtaaagtcg cggcgtaaag acggctcacc tcctgtcagg cgcacttttt cggtgcccag
     3301 actggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc ctttattggt
     3361 gacgccgctc ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc
     3421 ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc
     3481 catgtacacc tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat
     3541 ctttacgagc attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga
     3601 gtgtttagtg gttaagccga tactagcgtg taaatgagat ttttaccatc cacactttcg
     3661 ctatgcaatc atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat
     3721 ggcagtttta ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagaggt
     3781 agaagaaaat agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg
     3841 cgctactgta gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct
     3901 gatcttgatc gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca
     3961 ctttcgtctt tgggttctcg tttaacgggt atcgtcacca ccacggataa tggtggctcg
     4021 acggggcgta ttcgccgttc agaaggcggc attgcctggg gcgatatgcg caactgcctc
     4081 aaccaactga taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg ttttggcggc
     4141 aatggcgaac tttccgggca caaccttgga aacttgatgt taaaggcgct ggatcacctt
     4201 agcgtgcggc ctctggaagc catcaattta attcgtaatc tgctgaaagt ggatacgcat
     4261 ttgattccaa tgtcagagca tcccgtcgat ctgatggcga ttgacgatca ggggcatgaa
     4321 gtttacggcg aggtcaatat cgaccagtta actacgccga ttcaagagtt attattaacg
     4381 ccgaatgtac ccgcaacgcg tgaggcagtt cacgctatca atgaagcgga tctcatcatt
     4441 attgggcctg gcagttttta taccagcctg atgccaattc tgctgctgaa ggaaatcgcc
     4501 caggcattac gccgcacgcc agcgccgatg gtttatatcg gcaatctggg gcgtgagttg
     4561 agtttacctg cggctaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt
     4621 ggtaaaaaag tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa
     4681 gagcggattg tgatccagga ggtactggag gccagcgata ttccgtatcg tcatgaccgc
     4741 cagttgttac ataacgcgct ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa
     4801 ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg
     4861 aacgccttat ccggcctaca aaccgcgcaa attcaatata ttgcggagaa aatgtaggcc
     4921 tgataagcgt agcgcatcag gctgttttcc gtttgtcatc agtcttcttc gctatcctgt
     4981 tacgatgcgg caataaacaa ctcacgcaac tgatgcaact ggtcacgaat ttgtgccgct
     5041 tcttcgaact ccagattctg cgcgtgttgc atcatcagcc cttccagttc atggattttc
     5101 tgctgcaacg ctttcggcga catatccatc ggcacattat ccggctcaac aatcgggcgc
     5161 gattttcctc tgcccttcgc tttggttttg gcaatgttct gccccagcgc caggatatcg
     5221 accactttct tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt
     5281 ttctcacggc gacgttcggt ttcgccaatc gctttcgcca ttgatggggt gatcttatcg
     5341 ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac gaccaatggt ctggatcaac
     5401 gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga ggatcgccac cagcgacact
     5461 tccggcatat ccagaccttc gcgcagtaag ttgatcccta ccagcacgtc gaactcaccc
     5521 agacgcaagt cgcggataat ctccatacgt tcgacggtgt cgatatctga gtgaagataa
     5581 cgcacgcgct cgccatgttc ttcgagatat tcggtaagat cttccgccat ccgcttggtc
     5641 agtgtggtga ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga
     5701 tcatcaacct gtgtctccac cggccgcact tcgataatcg ggtcaagcaa tccggttgga
     5761 cgcaccacct gatccaccac atcgccgccg gatttttcca gctcgtaatt acccggcgtc
     5821 gccgaaacat agatggtttg cggcgctaat gcttcgaact cttcaaactt aagcggacgg
     5881 ttatccagcg ctgatggcag gcggaagccg tactccacca gtgtctcttt acgcgcccgg
     5941 tcaccgcgat acatgccgcc aatttgtgga atggtgacgt gagattcatc gacgaccagc
     6001 agcccatcgg caggcaggta atcaaacagc gtcggcggtg gctcacccgg tccacgaccg
     6061 gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt catcatctcc
     6121 agatcaaact gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt gttttccaac
     6181 agcactttgc gtctggcggc cagttcttct ttgatctcct ccatcgcctg tacgatgcgc
     6241 tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa aacgtggaat agtggaaaca
     6301 atctgcccgg tcagcgggtc aaataacgac aatcgttcca cttcctcgtc aaacagttcc
     6361 acgcgaagtg caatgtcatc cgattctgcc gggaagatat caatcacctc gccacgaacg
     6421 cggaaagtac cacgctggaa tgcttgatca ttacgagcgt attgcagctc cgccagtcgg
     6481 cgcagaatcg cgcgctgatc gataatcata ccgaccgtga gatggagcat catcttgaga
     6541 tataaatcag gatcgcccag accataaatc gcggaaacag aggccactac aaccacatca
     6601 cgccgctcca gcatcgcttt ggtggcggat aaacgcatct gctcaatatg ttcgttaacc
     6661 gaggcatctt tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg ctgatagtag
     6721 tcgtagtagg agacgaaata ctccaccgcg ttttccggga agaactcttt catttcgcca
     6781 tacagctggg ccgccagcgt tttgttgggc gcaagtacca tggttgggcg ctgaaggtca
     6841 gcaatgacat tggcaatggt gaaggttttc cctgagccag tcacgccaag taacgtctgg
     6901 tgcgccaggc catcttccag cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca
     6961 gaaggtttaa aagcggaatt cagtttgaac ggtttactca tgagtcgcta cctgaaggag
     7021 ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat tatgccaaca aattatactg
     7081 gataaaaaaa cagttcatca ccataatatt tctgatacag cgtaaactcc gctgtcaatc
     7141 atgagcaaaa tttactctgt ggcgagataa aactccggcc ttaccgggtt atccccaaag
     7201 caacggcttt tttaacattt gtcaagatga gtgatgacag ttttatggca agagatgcct
     7261 gttcagtgac tgccattgca tttatctaac cagttaaaaa ataaaagata tttctttgag
     7321 ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc tcgcttgcct cgtgttttct
     7381 aactctaata cacatggtta tccacaggaa tagtggataa ctgtctccag cccctatccc
     7441 ccgccgcctg ggcaattccc acattcgccg catgaggcgg aagtaaaatt tttttcgtga
     7501 ttattttaga atttaattgg ttaaattgca gtcaatcgaa gacgcgatct cgctcgcaat
     7561 ttaaccaaat acagaatggc tacaacaagg caaggtttat gtactttccg gttgccgcat
     7621 tttctggatt ttctgcaagc caggggatct ctcccagcag cggcgcggga atcatgcggg
     7681 tgagcgtggt catatattca gcgtgacgtt ttcccggagg cgtaacatcg ttcgccaccc
     7741 aacccgccag agtcagtccg gcgtgttgta ttgcctgcgc agtcaacatc gcgtgattaa
     7801 tacagccgag tttcacacca actaccagta tcaccggcag ttgttcctgt gttacccaat
     7861 ctgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc gccagcacct tccactaaca
     7921 cccagtcagc ctgttgttca agcgcgcgta atccggcgct cattaccaat gattctatcg
     7981 gcctgccctc ttgcgcgctg atgatgtgcg gcgaagtggg ttctgcgaag gtgtaaggat
     8041 ttactgttgc gtaatccagc tgcaggctgc tgttgtgctg taacgccagc gcgtcactat
     8101 tacgtaagcc ttccggggtc ttttcgctgc cagaggcgac cggtttataa cctgccgtcc
     8161 gataacctac tgccttcgcg gcttgtaaaa gtgcacaact ggcgacggtt ttccccactt
     8221 cggtatccgt tccggtgaca aaataacgtt tactcacgag caatcactcc caaaaaaaga
     8281 tgatacgtca gaggatatcg cccctgttgt tgcggccagg ccagttgcaa tcgctgcaat
     8341 tgcgaacgcg ttaatattcg cgggtcgcgc ccttcatgaa gatgcgtagc accgatgcct
     8401 ttcagcgaac gcatggcact gagcgcatca tcaaaccata gtgtgatggg ctgaatatga
     8461 tgttgataat gcacgccgtt cagcgactgt tcgatttcat ctggcggtaa aaagcgatta
     8521 gcatgcggac gctcgtccac cgcctgccac gcctgatgca gttcgggtaa cgatccctgc
     8581 accagcgtgg taaacgccac cacgcctccg gggcgcacca cccgatacag ctcgcggagt
     8641 gccgtggata aattaccgca ccactgcacc gcgagattgc tccatgcaag atcgaacgtc
     8701 gcagtcgcta acggcaggga ttcgatatct cccgccagat aatggtctgc agcatctttc
     8761 tggcgggctt gcacaagcat tggcggtgag agatctaacg ccgtcacctg tgcgtgacgc
     8821 tctcgccagt ggcggctcat ccagcctggc ccacaccccg cgtccagtac gtgggtgtat
     8881 ttacgctgtg ggagcattgc cagtaagacg tcagcactcc ggcgctgtaa ctctgcgtgt
     8941 tgctcatagt gcgcggctgc ccgaccaaat gccgctgcaa tggcttgttt attaaccgtt
     9001 gccatgcagc acctccagca gacggtcgat atcctgcatt tcatgcgctg cggttagcgt
     9061 taagcgcagt cgcgcagtac cagcgggtac ggttggcggg cgaatcgccg tgacccagca
     9121 gccttgctgg cgcagttttt ctgccagttg taacgcacgg ctgttatcac cgacgatcaa
     9181 tggctggatg gcgctgcatg aatcagcaag cgtaaacggc aaatcctgta ctccggcacg
     9241 aaaacgcgca atgagtgaca ccagtttttc gcgccgtgca tccccctcat cacggcgaat
     9301 gaccgccagc gacgcacgta atgcctgcgc ctgagcgggc ggcatactgg tgttgtagat
     9361 aaggtggcgg gcgaattgca gcagataatc cgccaccgta ctggagcaaa gcaccgctgc
     9421 cccgctgacg ccaaatcctt tgccaaaagt caccaccagc aattctggtt ttaccttttg
     9481 cagccagcag ctgccgcgcc cctgctcccc gataacgccc gtgccgtggg catcatcgac
     9541 catcaaccag ccattgtgct gttgcgttac ctgctggatt tccgccagtg gcgcactatc
     9601 gccgtccatg ctgaacacgc cttctgtcac caccagttgc tgccccggac agggggaagc
     9661 aagcagtcgc gccagatgag tgacatcgtt atgaacaaaa cggcgaagct gcgacgggct
     9721 taaactggca gcctccagca atgaggcatg gctaagccgg tcagcgacaa tgcggtcctc
     9781 ttttgccatc atcgcggcaa tgaccgcctg attagcggca aaaccagaga taaatagcag
     9841 tgcccgcgaa tagcccagcc actcggccag ttcttcttcc agcgcctgat gcgcaacgct
     9901 ataaccgctg acgtgaccgg aaccgccgct accgacgcca aattgctccg caccctgctg
     9961 ccaggcacgg ataatttgcg gatgatggct taaacctaaa taatcgttac tggaaaagtt
    10021 cagatactgg cgatcatccg ccaccagcca gcgtccggct ccttgcgcca ctggataacg
    10081 gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc gcgttgattt tctcctgcca
    10141 gctcatagtg ctgccgcgtt gtaatattcg tcagtatccg gggtcatcag cgcctgttca
    10201 agacgttgct gttgttcgtt atcccctgcc agcacggcag tttgctgcgg atttagcccc
    10261 agtttgcgga acagttgcag gtctttatct tcttccggat tcggcgtggt cagcagtttg
    10321 caaccgtaga aaatcgagtt tgcgcctgcc ataaagcaca tcgcctgagt ctgttcgttc
    10381 atctgctcgc gtccggcaga aaggcgcacg taagaggtcg gcatcatgat ccgcgcgacc
    10441 gcaatggtgc gaataaaatc aaaggcatca acgtcatcgt tatcggcaag cggcgtgcct
    10501 ttcaccttca ccagcatgtt gattggcacg ctttccggcg gcgtcggcag gtttgccagt
    10561 tgcagcaata atccggcgcg atcttttacc gtttcgccta agcccacaat gccgccagaa
    10621 cagactttga tcccggcatc gcgcactttt tccagcgtat cgaggcgttc ctgataagtg
    10681 cgggtggtga tgatattgcc gtaaaactcc ggcgaggtgt cgaggttgtg gttgtagtaa
    10741 tccagcccgg cgttcgcgag gcgctgcgcc tgagattcac tcaacgtgcc cagcgtcata
    10801 cacgcctcca gccctaaatc ttttaccccc tgcaccatct gctccaggta cggcatatcg
    10861 cgttcattgg gattcttcca cgccgcgccc atgcaaaaac gcgtcgatcc cgccgctttc
    10921 gctttgcgcg ccgactccag cacctgttca acttccatca accgctcggc ttccagaccg
    10981 gttttatagc gcgagctttg cgggcaatat ttgcaatctt ccgggcaagc accggtttta
    11041 atcgacagca acgtgctgac ctgcacctga cgaggatcga aatgctggcg atgcacctgc
    11101 tgcgcttcaa acagcagatc cagcaacggt ttttcaaata attctgcgac ttgcgacaat
    11161 gtccagcgtg ggcggtgagc catggggctt ctccaaaacg tgttttttgt tgttaattcg
    11221 gtgtagactt gtaaacctaa atcttttcaa tttggtttac aagtcgatta tgacaaagga
    11281 cgatcttgcc tttgaccaac gccatatctg gcacccatac acatccatga cctccccgct
    11341 gccggtttat ccggtggtga gcgccgaagg ttgcgagctg attttgtctg acggcagacg
    11401 cctgattgac ggtatgtcgt cctggtgggc ggcgatccac ggctacaatc acccgcagct
    11461 taatgcggcg atgaagtcgc aaattgatgc catgtcgcat gtgatgtttg gcggtatcac
    11521 ccatgcgcca gccattgagc tgtgccgcaa actggtggcg atgacgccgc aaccactgga
    11581 atgcgttttt ctcgcggact ccggttccgt agcggtggaa gtggcgatga aaatggcgtt
    11641 gcagtactgg caagccaaag gcgaagtgcg ccagcgtttt ctgaccttcc gcaatggtta
    11701 tcatggcgat acctttggcg cgatgtcggt gtgcgatccg gacaactcaa tgcacaacct
    11761 gtggaaaggc tatctgccag aaaacctgtt tgctcccgcc ccgcaaagcc gcatggatgg
    11821 tgaatgggat gaacgcgata tggttggctt tgcccgcctg atggcggcgc atcgtcatga
    11881 aatcgcggcg gtgatcattg aaccgattgt ccagggcgca ggcgggatgc gcatgtacca
    11941 tccggaatgg ttaaaacgaa tccgcaaaat gtgcgatcgc gaaggtatct tgctgattgc
    12001 cgacgagatc gccaccggat ttggtcgtac cggcaaactg tttgcctgtg aatatgcaga
    12061 aatcgcgccg gacattttgt gcctcggtaa agccttaacc ggcggcacaa tgaccctttc
    12121 cgccacactc accacgcgcg aggttgcaga aaccatcagt aacggcgaag ccggctgctt
    12181 tatgcacggg ccaactttta tgggcaatcc gctggcctgc gcggctgcaa acgccagcct
    12241 ggcgattctc gaatctggcg actggcagca acaggtggcg gctattgaag tacagctgcg
    12301 cgagcaactt gcccccgccc gtgatgccga aatggttgcc gatgtgcgcg tgctgggggc
    12361 aatcggcgta gtcgaaacaa ctcgtccggt gaatatggcg gcgctgcaaa aattctttgt
    12421 cgaacagggg gtatggatcc ggccttttgg caaactgatt tacctgatgc cgccctatat
    12481 tattctcccg caacagttgc agcgtctgac cgcagcggtt aaccgtgcgg tgcaggatga
    12541 aacatttttt tgccaataac gggaagtcag cgtgtgggtt tctggctaca ctttctgcaa
    12601 actagaaagg agggttcatg aaactcatca gtaatgatct gcgcgatggc gataagctgc
    12661 cgcatcgtca tgtctttaac ggcatgggtt acgatggcga taatatttca ccgcatctgg
    12721 cgtgggatga tgttcctgcg ggaacgaaaa gttttgttgt cacctgctac gacccggatg
    12781 cgccaaccgg ctccggctgg tggcactggg tagttgttaa tttacccgct gatacccgcg
    12841 tattaccgca agggtttggc tctggtctgg tagcaatgcc agacggcgtt ttgcagacgc
    12901 gtaccgattt tggtaaaacc ggatacgatg gcgcagcgcc gccgaaaggc gaaacccatc
    12961 gctacatttt taccgttcac gcgctggatg tagagcatat tgatgtcgat gaaggagcca
    13021 gcggcgcgat ggtcgggttt aacgttcatt tccactctct ggcaagcgca tcgattaccg
    13081 cgatgtttag ttaatcactc tgccagatgg cgcaatgcta tctggtatca cttaaaggta
    13141 ttaaaaacaa ctttttgtct ttttaccttc ccgtttcgct caagttagta taaaaaagca
    13201 ggcttcaacg gattcatttt tctatttcat agcccggagc aacctgtgaa cacattttca
    13261 gtttcccgtc tggcgctggc attggctttt agcgtgacgc tgaccgcctg tagctcaaca
    13321 ccacccgatc aacgtccttc tgatcaaact gcgcctggta cctcttctcg cccgattctg
    13381 tcggcaaaag aagcgcagaa tttcgatgct caacactatt ttgcatccct gacaccaggt
    13441 gctgcagcgt ggaatccttc cccgattacc ctgcctgcgc aacctgactt tgttgtcggc
    13501 ccggcgggca ctcaaggtgt aacgcatacc acgattcagg cggcggtaga tgcggcaatt
    13561 atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc ctggtgagta tcagggaacg
    13621 gtgtatgtcc ctgctgctcc gggtggaatt actctgtacg gtacaggtga aaaaccgatt
    13681 gatgtgaaga ttgggctttc ccttgatggg ggcatgagcc ctgccgactg gcgtcacgac
    13741 gtcaacccgc gcggcaaata tatgccaggt aaaccagcgt ggtatatgta cgatagctgc
    13801 cagagcaaac gcagcgacag tatcggtgtt ctctgctctg cggtcttctg gtcacaaaac
    13861 aatggcctgc aactgcaaaa cctgaccatc gaaaacacgc tgggcgatag cgtagatgca
    13921 ggtaaccatc cggcggtggc actgcgtact gatggtgacc aggtacagat taacaacgtt
    13981 aacattctcg gtcgtcagaa caccttcttt gtcaccaaca gcggtgtgca gaaccgtctg
    14041 gaaacgaatc gtcagccgcg tacgctggtg accaacagct acattgaagg ggatgtggat
    14101 atcgtttctg gtcgcggcgc agtggtgttc gataacaccg aattccgcgt ggtgaactca
    14161 cgtactcagc aagaagcgta tgtgtttgca ccggctacgc tgtccaacat ttactacggt
    14221 ttcctcgccg taaacagccg tttcaatgct ttcggtgatg gtgtggcgca actgggccgc
    14281 tcgctggatg ttgatgccaa taccaacggt caggtggtga tccgtgatag cgccatcaac
    14341 gaaggtttta acacggctaa accgtgggcc gatgcggtga tctctaatcg tccgtttgcg
    14401 ggtaataccg gcagcgtaga tgataacgac gaaatacagc gcaatctgaa tgacactaac
    14461 tacaaccgca tgtgggaata caataaccgc ggcgtgggta gtaaagtggt tgcagaggcg
    14521 aagaagtaag agcaattaac tatttgccgg atgcggcgta aacgccttat ccggcctacg
    14581 gttcgatgcg atttgtaggt cggataagat gcgcaagcat cgcatccgac aataagtgcc
    14641 gaatgcgacc tacattcaca tggcgctttt tacatctgac ggtttttatt gaagttaatc
    14701 aaactacccg ccttgataat ctcgcgctct tcagcagtca gactttccat atagagcgta
    14761 atttccgtta ccggcgcatc ttcatggatc acataacctt taaacgtcgt acccggatta
    14821 tccagcgccg ctttaatgcc agggatgtaa atgtaatccc ccacttcaaa ggttggcact
    14881 tccgccatct gcagcggtaa catcccccag ttgatgacgt tagaacggta gcgtttagtc
    14941 gcgtactctt cggcaatatt cgccagaccg ccaatcacac gctggcagct cgccgcctgt
    15001 tcacgcgcag aaccatcgcc tggtttcacc gcatatacca tgctgccaat ttcagtttgc
    15061 agcggatcaa tatgctcctg accagcaatc tgcttaatgc gcgcaaacac ctctgtcagc
    15121 tcgctgacat tccccgccag acgctgattt tccagctcag cagtcgcttt acttctgcca
    15181 acataaccgg gatcgcggcg tgacagggta aactccgcca gaccaatcgg atttgaacga
    15241 taagaagagg tttcaccgga aggaatcagt tcgtcggtgg tggtcacttc gtcgaggatc
    15301 ttcgagcaca ctttcaggac gatattgtca gtcagcgcac ccaattccgg ccagtcttta
    15361 atgttcggtc cgtaaatcag cggttgctga gttgcccctt tcacaaagcc ctgataaaca
    15421 cggtttttat acggcgttac atcgaaggcg tactccggca cgttgtccca gcaatcaagt
    15481 tcgctggcag aggttaaata gccaccgttt gccgcagtcg cagcgataga acgagcgtcc
    15541 atcaacgcca ccgctgacat ctgcccatta gctggcttag agccttcgcg gttcgggaag
    15601 ttacgcgtgg tgtggcgaat acttaatccg ttgttgattg gcgtatcgcc cgcgccaaag
    15661 catgggccgc agaacgcggt tctgatgatt gcgcctgcgc caatcaaatc tgctaccaca
    15721 ccttttttgg ctaaatccat aaacaccggc tgtgatgacg ggtaaaccgc cagcgagaag
    15781 gtgtcattgc cacaggattg accgcgcagt gcattcgccg ccgcgatgac gttttcgtag
    15841 ttaccgccag aacagcccgc gataatcccc tgctgcactt tcaggcgacc attttccact
    15901 ttatccagca gcgagagttt ggctttaccg tgcgccacgc gttcggactc aatttcaatc
    15961 tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga tttcatacac gttgctcggg
    16021 tggaacggca gcgcaatcat tggtttgatg gcgcttaaat caacgctgat gcagccatcg
    16081 tagtacgcca tcggttgagg gttaagctgg cagtaatcct ggccgcgacc gtgcagcgcc
    16141 agccagttat gcacttcttc atcagtttgc cagacggagc ttaaacaggt ggtttccgtg
    16201 gtcatcacgt caacgctgtt acggaaatcg gtagagagcg cgctaacgcc cggtccaacg
    16261 aactccatga ctttgttttt gacgtaaccg tttttgaaca ccgcgccaat gatagccagc
    16321 gccacatcct gtggccccac atacggcgca ggttttccgg tcagatgcac cgcaaccacg
    16381 cccggatagt cgatatccca ggtgtcatta agcagctgtt ttaccaactc accgccgccc
    16441 tcaccgactg ccattgtccc taatgcaccg taacgggtgt ggctgtctga cccgaggatc
    16501 attttgcccc cgcctgccat catctcacgc atatattgat ggatgatcgc aatatgcgga
    16561 ggcacaaaaa taccgccata acgctgagct gccgataaac caaaaacatg gtcatcaccg
    16621 ttaatggtgc cgccgacggc gcagagtg
//
LOCUS       DAJVTY010000090        16613 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVTY010000090 DAJVTY010000000
VERSION     DAJVTY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16613
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.090"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..246)
                     /locus_tag="OQQ66_002536"
     CDS             complement(<1..246)
                     /locus_tag="OQQ66_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1905424.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPIVTWIV"
     gene            353..649
                     /locus_tag="OQQ66_002537"
     CDS             353..649
                     /locus_tag="OQQ66_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1905425.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(783..1208)
                     /gene="arsC"
                     /locus_tag="OQQ66_002538"
     CDS             complement(783..1208)
                     /gene="arsC"
                     /locus_tag="OQQ66_002538"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1905426.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1221..2510)
                     /gene="arsB"
                     /locus_tag="OQQ66_002539"
     CDS             complement(1221..2510)
                     /gene="arsB"
                     /locus_tag="OQQ66_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1905427.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2565..2918)
                     /gene="arsR"
                     /locus_tag="OQQ66_002540"
     CDS             complement(2565..2918)
                     /gene="arsR"
                     /locus_tag="OQQ66_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1905428.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3657..3740
                     /locus_tag="OQQ66_002541"
     CDS             3657..3740
                     /locus_tag="OQQ66_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1905429.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3794..5146)
                     /gene="gorA"
                     /locus_tag="OQQ66_002542"
     CDS             complement(3794..5146)
                     /gene="gorA"
                     /locus_tag="OQQ66_002542"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1905430.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5218..6060)
                     /gene="rlmJ"
                     /locus_tag="OQQ66_002543"
     CDS             complement(5218..6060)
                     /gene="rlmJ"
                     /locus_tag="OQQ66_002543"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1905431.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6263..8305
                     /gene="prlC"
                     /locus_tag="OQQ66_002544"
     CDS             6263..8305
                     /gene="prlC"
                     /locus_tag="OQQ66_002544"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1905432.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8313..9065
                     /gene="rsmJ"
                     /locus_tag="OQQ66_002545"
     CDS             8313..9065
                     /gene="rsmJ"
                     /locus_tag="OQQ66_002545"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1905433.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9114..10583)
                     /gene="dtpB"
                     /locus_tag="OQQ66_002546"
     CDS             complement(9114..10583)
                     /gene="dtpB"
                     /locus_tag="OQQ66_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS1905434.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10901..11335)
                     /gene="uspA"
                     /locus_tag="OQQ66_002547"
     CDS             complement(10901..11335)
                     /gene="uspA"
                     /locus_tag="OQQ66_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1905435.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11726..12061
                     /gene="uspB"
                     /locus_tag="OQQ66_002548"
     CDS             11726..12061
                     /gene="uspB"
                     /locus_tag="OQQ66_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1905436.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12305..13804)
                     /gene="pitA"
                     /locus_tag="OQQ66_002549"
     CDS             complement(12305..13804)
                     /gene="pitA"
                     /locus_tag="OQQ66_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1905437.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14036..15238
                     /locus_tag="OQQ66_002550"
     CDS             14036..15238
                     /locus_tag="OQQ66_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1905438.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15553..16605)
                     /locus_tag="OQQ66_002551"
     CDS             complement(15553..16605)
                     /locus_tag="OQQ66_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCS1905439.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4269 a         4235 c         4312 g         3797 t
ORIGIN      
        1 aacaatccac gtaacaatgg gtattgtcga gcaactacag aacggggtaa cggctcccaa
       61 cagagcagcc aggagatagc ctctcccttt tctggccccc atcatctgct ggattttatg
      121 gtctggcacc ttttgtctta tcaggctgac accggcacta atcactataa acagcaacga
      181 aagctcgacg gcgagaaaca caaacatctc tgcggcatct tgcagcatcg caagccaact
      241 actcatacgt aacctcattg gataaatcta tatttccaga ataatcgaaa tgaaattcag
      301 atcaagatat ttctggtatt atcgaaatat caaccagaca gaggggtgtc atatgcagtt
      361 ggaagaagtc gctaaagcgc tgaaagagct aggtcacccg acccgtttgt tcatatttaa
      421 acatcttgtc aaagccgggg aacaagggtt gccggtcggt gagttgcaaa agcagcttgg
      481 gatacctggc tccacactca gccaccatat atcagcactg gtatcggtag gactggtcaa
      541 acaaaaccgt gagagtcgga cgctgatgtg tgtttcgcaa tacgcaatgc tggaagcgat
      601 aattgaattc ttacgcgagg agtgctgcgt aaatagcaaa attgcctgat gcgctacgct
      661 ctacatattt catgccatat attgaatttg cacggttttg taggtcggat aagacgcgtt
      721 agcgtcgcat ccgacataaa caaagcgcac tttaccaact atcggcagtg gatatcgccg
      781 ctttatttca ggcgcttacc cgcttcatca accactttct cgccatcttc cttggagaat
      841 gcgccttttt gcgcatctgg cagaatttcc agcaccactt ctgaagggcg gcacaggcga
      901 gttcccagcg gcgtcaccac aatcgggcga ttaatcagaa tcgggtgctg aagcataaag
      961 tcgattaacc gatcgtcagt aaatttatct tccgcaaggc ccagctcctc atacggttcg
     1021 acgtttttac gcagcagcgc gcgtacggaa atccccatat cggcaatgag tttgaccagt
     1081 tcatcgcgcg ttggcggagt ttccagataa tggataatgg tcggttctgt gccgctgttg
     1141 cggatcatct ccagcgtatt acgcgacgtg ccgcaggccg ggttgtgata aatggtaatg
     1201 ttgctcatat cagtatctca ttacaaagtg aaagagagac gtagcgccag cgcagccagc
     1261 gtcacaaaca gcacaggcag ggtcatgata atccctgtac ggaagtaata tccccagctg
     1321 atagtcatat tcttctgcga aagtacgtgc agccagagta gtgtagccag gctaccaatt
     1381 ggggtaattt tcggtcccaa atcgcagcca atcacattgg cataaaccat cgcttctttg
     1441 ataacgccag atgccgtgct gccatcaatg gacaacgcgc caaccagtac cgtcggcata
     1501 ttgttcataa tagaagagag gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac
     1561 aggccgttat ccgccagcac gttgagtacg ccagaaagat attccgttaa tccggcattg
     1621 cgcaggccat aaaccaccag atacatgccg agcgagaaga tgacaatttg ccagggggca
     1681 ccgcgcagga ctttacccgt attaatcgca tgaccgcgtt tagcgacgac aaataatatc
     1741 agcgcgccca cagctgcaat ggcgctcacc ggaatgccga gcggttccag gacgaaaaat
     1801 cccaccagca gaagcagtaa aacaacccag ccagttttga acgtagcagg atctttgatc
     1861 gcttctgcgg gagatttcag cagcgccata tcgtagttct gcggaatatc tttgcgaaaa
     1921 tagagatgta gcatcaccag cgtggcaaca atcgcggcga tatccaccgg caccatcacc
     1981 gaggcgtatt cgcgaaagcc gaggccaaag aaatcagcgg aaacgatatt caccaggttg
     2041 gagacaataa gcggcaggct ggcggtatcg gcaatgaatc cggccgccat cacgaacgcc
     2101 agcgtcgtgc ctttactgaa tcctaaagcc agcagcatgg cgatgacaat cggcgtcaaa
     2161 ataagcgctg cgccatcatt ggcaaacagg gcggcaacgg cagcaccgag caggacaatc
     2221 caggtaaaca gcaagcgacc acgaccatta ccccagcgtg agacgtgcag cgccgcccat
     2281 tcaaaaaagc cggactcatc cagcagcagg ctgatgataa tgacggcgat aaacgcagcc
     2341 gtcgcgttcc agacgatatt ccacaccacc ggaatatcac ccggatggac cacgcccgta
     2401 actaacgcca gtactgcgcc gagcgttgca ctccagccga tgcctaaacc tttcggctgc
     2461 cagataacca atacaatggt caggacaaag atagcgcctg ccagtaacat aatgcctcct
     2521 ggataaaaca catgcgaaaa tacatatgtg tttagctaaa ttttttaact gcaaatgttc
     2581 ttactgtctg cggaacagtt ttgtcgagcc aggttgcgga caattgcctg aatcttttcc
     2641 tgttcacatc gccaggcttc atcaattatt ttcgccgccc atgctggaat atgcggcgat
     2701 aagcggtaat gaacccattt accttgcttg cggtccagca atagcccgct ttcacgcagc
     2761 aatgccaggt ggcgggagat cttgggctgc gactggtcga gagcagtgca gagatcgcag
     2821 acgcataact ctcccagttc gctgagcagt aaaacgatgc ccagacgggt ttcatcagca
     2881 agaattttga acaattggat gggtaacaga aatgacatat tgcgctcctg attgttgcag
     2941 gtagtgtctc tcttcgaagc ggataagtca aaaacatata tgatttaacg aatgtgtaag
     3001 tgcagaggaa ggtaataggt gtgaattttg agttggctat tcatttgaaa ggaggtctga
     3061 atcaggacgt aaaaaaaccg actttgcgtc ggttttttac tttccagccc tgagttggtg
     3121 gctctggctg gagtgagaga gttcttatct aacagctcaa tactattagc cagtggctaa
     3181 cattgcaaga attagcgatt tcttcagctg gcggagggag cacttcggta tatatcgcag
     3241 tgacgattta tatactttca ctgggtcatc gtcatattaa gccttgtccg aatccggatg
     3301 taaaaaaccg actttgcgtc ggttttttac tttccagccc tgagttggtg gctctgactg
     3361 gagtgagata gccatcatct aacgcatcaa tgctaatacc atactgaaac tattgcaagg
     3421 acgtgctggt tttataacct gcatgtactg tatgattatc cagttagctc tgaggcattt
     3481 tcactctggc aatgcgcata aacgctttca aagtcctggt cagaagtacg ggtggtgccg
     3541 ttaactgatg ctctggccgg agtgagagag ttcttatcta acaatgagac atgcgccgtg
     3601 acaggcagtg gatgagtaag cggatgcatt ctcactccat cgcatggaga aaacgggtga
     3661 ttgataaagc aatcatcgtt ctaggggcgt taattgcgct gctggaactg atccgctttc
     3721 tgcttcagct tctgaactga tagcggaaac gtaattaagg gctaagagca cactactctt
     3781 agccctttaa catttaacgc attgtcacga actcttctgc cgccgttggg tgaatggcga
     3841 cggtattgtc gaagtctttt ttggttgccc ccatcttcag cgccaccgcg aagccctgca
     3901 acatttcgtc cataccaaag ccaatgccgt gaataccgac aatcttctct tccgatccaa
     3961 cgcacaccag cttcatgcgg cacggctggc ggtgagtggt gacggcggta tacatcgcgg
     4021 tgaaagagga tttatacact ttcacctgat cgtcgccata ctgctcgcgc gcctgcggtt
     4081 ccgttaaacc aacagtacca atcggcggat ggctgaagac cacggtcgga atgttgctgt
     4141 aatccagatg ctcatccggc ttgttattaa acaggtgttc agagagacgg cgacccgctg
     4201 caactgccac cggtgtcagc tccactgcac ccgtgttatc gcccaccgcg taaatacctt
     4261 caatgttggt gttttgatat ttatcgacga cgatatagcc tttttcgttg gttttaacgc
     4321 cagcggcttc gaggttgatg ttgtcattgg caggctcgcg accaatcgcc caaatcaggc
     4381 aatccaccgt ttcactgcga ccatcttcca gctccagcgt caggctacca tcggcatttt
     4441 tcactaccgc tttcgggatg gcgttggtgt gcagctgcgg gccttcggcg ttcatcactt
     4501 cgaccagcgt ttcggaaatc atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa
     4561 acagatgcgt tttcgcgccg aggccgttaa tcacgcccgc cagctcaacg gcgatgtaac
     4621 ccgcgccaac aaccgccacg cgctctggca aagcaggaag ggcgaagaag ccatcagaat
     4681 caataccgta ttccacgccc ggaatatccg ggtggctcgg acgaccgcct gtggcgatca
     4741 gaatatgatc ggccgtgatg gtttcgccgt ttacctccag cattttggca tcaacgaagc
     4801 gggcaaagcc tttgattaca tcaacgttat ttttaccgag cacgttttca taggaagtat
     4861 gaatacggtc gatataggcg gtacggctgg cgatcaacgt ttcccagttg aacttgttga
     4921 tggtggtatc gaaaccataa tcagggccgt acatatggat cgcttcacgg atttgcgccg
     4981 cgtgccacat cacttttttc ggcacgcagc cgacgttcac gcaggtgccg cccagctctt
     5041 tggcttcaat cagtgcgcat ttctggccgt acatagccgc gcggttgatg gaggctatac
     5101 cgccgctgcc gccgccgatg gcgatgtaat catagtgttt agtcatgaca gagtgtcctt
     5161 atcgttgatt accgcgattg tagcgcgaga cgtaataggt gccagcaatg gctgcaatta
     5221 ctccggcacg atccagctta cggtggcgtg cccggtgcct gccggaacca gtttgctgtg
     5281 cagccacggc agcacgttat tcatctgctg ttccagtttc cacggcgggt taatcacaat
     5341 catgccggaa gcggtcatgc cacggcgatc gctgtctggc agtaccgcca gttcaatttg
     5401 cagaattttg cgaataccgg tcgcttccag atcgtggatc atgcgcttaa tttgctgacg
     5461 cagcaccacc ggataccaca gtgcgtaagt accagtggcg aaacgtttgt aaccttctgc
     5521 tatcccgctg accaccgcct gatagtcggt tttcatttca tacggcgggt cgataaggat
     5581 taaaccacgg cgggaaaccg gcggcagttt ggccttaagc tgctggaaac catcggcttt
     5641 ttcgacgcgc gcacggctgt ctttctgaaa ttcagaacgc agcaacgggt aatcgctcgg
     5701 gtgcagttcg gtcagttgca ggctgtcttg ttcgcgcagt agctggcgag caatcaatgg
     5761 cgaaccgggg tagtaacgca actgaccgct acggttgaag tgttttacca cattgatgta
     5821 cgcctccagt tctgcgggca aatcgtcctg ctgccagata cgggcgatgc cttcgagata
     5881 ttcgccggta cgctcggcat gttcgctgcc taactgataa cgcccggccc ctgcgtgggt
     5941 gtcgagatag agaaacggtt tatctttctc tttcagcgac tcgatgatca ggctctgaac
     6001 ggtatgttta aggacgtcgg cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat
     6061 gggtaaaggt gttccggtaa gtaaaaatcg gcccgtattc ggggcgcaca aaagcgtatc
     6121 aggacagtat accgaaaaga ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc
     6181 actacactta accccatgct acacacatta tgtaaagcgc ctgttgagcg cttccttaac
     6241 ctctttaacc aggactgcgc gaatgacgaa tccgttactg actccctttg aattgcctcc
     6301 gttttctaaa attctcccgg aacatgtcgt tccagccgtg actcaggcgc tgaacgattg
     6361 ccgcgaaaat gtggagcgcg tagtagcgca aggggcaccg tacacctggg aaaatctctg
     6421 ccagccgttg gcggaagtgg acgatgtgtt ggggcgtatc ttctccccgg tcagccacct
     6481 gaactcggtg aaaaatagcc cggaactgcg tgaagcctac gagcaaaccc tgccgctgct
     6541 gtcggaatac agcacctggg tagggcaaca tgaagggctg tataaagcgt accgcgacct
     6601 gcgcgatggc gatcattacg ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc
     6661 cctgcgtgat tttgaactct ctggcatagg tctgccgaaa gagaaacagc agcgttacgg
     6721 cgaaattgcg acccgtcttt ctgaactggg caaccagtac agcaacaacg tcctcgatgc
     6781 gacaatgggc tggaccaaac tcgttaccga cgaagcggag ctggcgggga tgcctgaaag
     6841 cgcgctggct gcggcaaaag cccaggccga agcgaaagag ctggaaggct acctgctgac
     6901 gctggatatc ccaagctacc tgccggtaat gacctactgc gacaaccagg ccttgcgtga
     6961 agagatgtat cgcgcttaca gcacccgcgc ctccgatcaa ggcccgaacg ccggtaaatg
     7021 ggataacagc aaggtgatgg aagagatcct cgctctgcgt cacgaactgg cgcaactgct
     7081 gggctttgaa aactacgcct ttaaatccct tgccactaaa atggcagaaa acccgcagca
     7141 ggtgctggat ttcttaaccg atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct
     7201 ggcacaactg cgcgccttcg ccaaagcgga atttggcgtc gatgagttgc agccgtggga
     7261 tatcgcgtac tacagcgaaa aacaaaaaca gcacctctac agcatcagtg acgaacagct
     7321 gcgtccgtac ttcccggaaa acaaagcggt taacggcctg tttgaagtgg tgaaacgtat
     7381 ttacggcatc accgctaaag agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt
     7441 cttcgaactg tatgacgaga acaacgaact gcgcggcagc ttctacctcg atctgtatgc
     7501 ccgtgaaaac aagcgcggcg gggcgtggat ggatgactgc gtaggccaga tgcgtaaagc
     7561 tgacggttct ctgcaaaaac cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa
     7621 tggtaaaccg gcgctgttca ctcacgacga agtgatcacc ctgttccacg agttcggtca
     7681 cggcctgcac catatgctga cccgcatcga aaccgctggt gtttccggta tcagcggtgt
     7741 gccgtgggat gcggtcgaac tgccgagtca gtttatggaa aactggtgct gggagccgga
     7801 ggcgctggcg tttatctctg gtcactatga aaccggcgaa ccgctgccga aagagttgct
     7861 ggataaaatg ctggcggcga agaactacca ggcggcgctg tttattctgc gccagctgga
     7921 gttcggcctg ttcgatttcc gcctccatgc cgagttccgc ccggatcagg gtgcgaaaat
     7981 cctcgaaact ctggcagaaa tcaagaaact ggttgccgta gtaccgtctc catcctgggg
     8041 ccgtttcccg cacgctttca gccatatttt cgccggtggt tatgccgcag gttactacag
     8101 ctacctgtgg gctgacgtac tggcggcaga tgccttctcg cgctttgagg aagagggcat
     8161 tttcaaccgt gaaaccgggc agtcgttcct cgacaacatt ctgagccgtg gcggttcaga
     8221 agagccgatg gatctgttca aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct
     8281 ggagcattac ggcattaagg gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag
     8341 gcgccggaga cggcgcctta tctgttctgg cggcccgctg ggggctggag cacgatgaag
     8401 acaacctgat ggcgctggtg ttaacgccgg aacatctgga attgcgcaag cgtgatgagc
     8461 caaaacttgg cggcatcttt gttgattttg ttggcggagc gatggcgcac cgacgcaaat
     8521 tcggcggtgg tcgcggtgag gcggtggcga aagcggtggg cattaaaggc gattatttgc
     8581 cggatgtggt ggacgccacc gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg
     8641 gctgccgcgt gcggatgctg gagcgtaatc cagtggttgc cgcgctactc gacgacggcc
     8701 tggcgcgtgg ttatgcggat gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa
     8761 ttcacgcctc cagcctgacg gcgctaaccg atattacccc acgcccgcag gtggtctatc
     8821 tcgatccgat gttcccgcat aagcagaaaa gcgcgctagt gaaaaaagag atgcgtgtct
     8881 ttcagtcgct ggtgggaccg gatcttgatg ccgatggatt actggagcct gcacgcttgc
     8941 tggcgaccaa acgcgtggtg gtaaagcgcc cggactacgc gccaccgcta gcgaatgtca
     9001 ccacgccaaa cgcggttgtg actaaagggc atcgctttga tatctatgca ggtacgcccg
     9061 tgtaaaagta aagcagggta gcgataacgc taccctgctt tctgcaagaa taattaatgg
     9121 ctttccggcg tcgcaatcat gcgtttcagc cacggcacca tcaacagcat cactactgca
     9181 acgcccagcg tgaccagacc aatcttacca aacacgttgg tatagacggg caacgtctca
     9241 agcggatcgg taatgttgtc cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag
     9301 aacgcggcag cctgcgtcag gaaccacatc ccgagaataa agcccatcaa atgctgcggc
     9361 accagggcag caatcatcgc caggccaagg gcgctaataa acagttcacc taagctctgg
     9421 aataagtaca ccagcacgat aaaccatggc gatgtcagcc cttgtgcatc cgcaaaccac
     9481 attcccgcag ctgccgccgt caaaaagccc agtgagcaca taaacatgcc gagagtaaat
     9541 ttcatcggca tcgagaggtc tttgcctttg ttacccagat gcgtgtaaat gcctgccagt
     9601 attgggctgg cgagtaccac ccagaacggg ttaagcgcct ggaagctgac cgagttgatg
     9661 gaaaaaccga gaatttcatg atgcacgttg ttgatggcaa agaagttcag cgatgttggc
     9721 atctgggcgt agagaatgta aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac
     9781 attttattgc gcccggtttt atccagcttg aatgcctgac gaaagaagat gatggtgacg
     9841 acgatggaga gaacaatcag caccagattg gcgacttcta cgttgtgcat cagccatgca
     9901 catacgaaga tcatcaccac gctgccaagt aatacgtaca acagtttgct gaagctcatc
     9961 ggcttgaagt cgggttcaga accaatgtct ttcaccattc cacgacaggc gatgtaaacc
    10021 agtaatgcga taattaaccc cgccccgcac aggttgtagg tgactgaata accgaatcta
    10081 tcagcgatca caggggccag cgataacgct atcaacgagc cgatattgat cgacatatag
    10141 aacagggtga atgcgccatc aagccgcgga tctttcggcg gatagcactt cgaaagcaag
    10201 ctggctgggt tagctttaaa caggccgtta ccgacagcga tagtccccag ggcgatgaaa
    10261 atcaggtcag gcttaagtag cgacatgccg gtcatgaagt agccaatcgc cagcacaagt
    10321 gctccgagaa caatggtgcg tttggtcccc agcaggtggt cgccgacata gccgccaatg
    10381 gaaatgaggc catagaccag cgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc
    10441 gagaatccaa gctgtttaac gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg
    10501 aatcgctccc ataactcgac aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc
    10561 cccatgggtg ttgttgtatt catatttatt aaccttccaa taccatcctg aaaagtatga
    10621 acgcgtaagg gataacgcgc gaaaaagttt acatctgtca gagattctta atattgatgc
    10681 ggtgaatgga ttaatacact attacaacag aaaataacca gctaatcaca ctaatgatgg
    10741 aaaatatgat ggtgttaaat gttgtattgg caggagattt tgattgcgta cgcagatcgt
    10801 aggccagata aggggtttac gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc
    10861 cgcatatgcg ggcgttcagg aacacgtcgt ggagggaaga ttattcttct tcgtcgcgca
    10921 gcggaacaat cagcatatca acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca
    10981 gtttgctcca gaagtcctgg tggtgaccac aaaccaccag atccatatcg tatttcttga
    11041 ttgcatcgac cagaacctgg cccaggtcgc cgctgccgct cagggtttca gtgattgggt
    11101 agcctgcatt agtggaaagc tcggtcagcg catgatgtgt ctcttcagag atgcgtttct
    11161 gcatatcacc cagattcaca tcaataagcc cggtgtatag gtcagagtag tttacatcta
    11221 cgtggatcag agaaactttc gcattgtagg ggcgagccat agagactgct ttctctacca
    11281 gaactttgct ttccggggag agatcgaccg cgatgagaat gtgtttataa gccatagtgt
    11341 tactccttcc ataaagttgt cgatgactgg ccagctagcg tttcttgtgc cgttacgtca
    11401 cccgcgtcct gcgaactacg tgcgcggggc taacccagcc gtaaagattc agtgttaaga
    11461 ccatccttac cttatagcga cccggatgat ccgtcaatcc gccttgctta cccgttagat
    11521 aaacaaattt tcgacatgaa tatattgata gtggttaacc ttctggaaaa aaaacaacct
    11581 gatctcctac actatctata gagccgctcg gatgttggtc accacaaaag cggtttctcc
    11641 aggacctggt gttaactgat tggcagcgta tcgggagcgg tagccccggg gtggaactcc
    11701 gcgggcaggt cgccggggag gaggtatgat aagcaccgtc gcattatttt gggctttatg
    11761 tgtcgtttgc attgttaaca tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt
    11821 actgcgtaac tgcgatccat tgctctatca atatgttgat ggagggggct tttttacctc
    11881 acatggccaa cccaacaaac aggtgcgtct cgtttggtat atctatgccc aacgttatcg
    11941 cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct
    12001 gactagcgca ttgtgtggtc tggtggtggt cagcctgatt gcattgatga tttggcatta
    12061 agcacttcag caataataac gcataaaaaa agctggccag taaactgacc cgctcgagac
    12121 tgattacaaa cgcaaaattg cctgatgcgc tacgcttatc aggcctgcat aatctctgca
    12181 atctattgaa tttgcatgtt tttgtaggcc gaataaggcg ttcacgccgc atccggcatg
    12241 aacaaagcgc actatgtcac aatctgaagc gggccagctg acccgctcgt tttggtgcgt
    12301 acgattacag gaactgcaag gagagccagt acagcccgcc ggaaagcagt accgcagccg
    12361 gaagggtaaa cacccaggcc atcagaatgc tggtcacggt tttacgctgt aagccgccac
    12421 catctaccac catcgtcccc gcgacagaag aggagagtac gtgagtagtg gaaaccggca
    12481 tcccggtata actcgccagg ccgatagaca ctgccgccgt catctgggca gacatcccct
    12541 gagcgtaggt catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc
    12601 agccaatcat cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggtg
    12661 catactcgat ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag
    12721 cactcacgcc aggtatcttc actactttgt cgatagtgtc agaaacgcac agcataatgc
    12781 ggcgcatctg gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtagtta
    12841 acataccttt caggcggttg agcgcgttaa tggtattcga cggatggcaa tggaactccg
    12901 cgggttgcgt tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt
    12961 tcagcagcgc aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac
    13021 gggtgatttc gtagccagtg gcattcatat tcaccacgaa gcctgctggt gcgacaccaa
    13081 tcaataccaa cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg
    13141 ccacgccgat agcggaaagg atcagcgcaa tacgcgtcca gaacggcggc tttttcttgc
    13201 cgtctttctt ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc
    13261 tccagtagcg acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg
    13321 aaacaatcag agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg
    13381 acgtcccggt catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagtgtat
    13441 gagagctgga tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca
    13501 gcaacataga gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg
    13561 tcggcagcat atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa
    13621 agttgaacac cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa
    13681 cggttgccac ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa
    13741 aagccagtgc aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta
    13801 gcataggacg ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg
    13861 gcgcgggcaa agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa
    13921 aaaataatta tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg
    13981 tgaccactga ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga
    14041 aaggtttgat gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc
    14101 aggtcaggca ggacgccggg ttctgctgat cgataatggt aaaaaaccag ggcgcaaaat
    14161 ccttatgtct ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta
    14221 tctgagccag aatccgcatt tttgtaagtc tgcactcgcg cgttttaccc agtgggattt
    14281 cattgatctg gtcaataaac acggcatcgc ctggcacgag aaaacgttag gacaactctt
    14341 ctgcgatgac tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa
    14401 tgtaaccttc agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac
    14461 gcttgaactg aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct
    14521 gtcaatgccg gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct
    14581 caacgtgctg ccgacccgtg cgggtctggt gccattcact ctgcataaac cgttgctcga
    14641 agagttacag gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac
    14701 cgttttccgt gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca
    14761 gatttccagc tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga
    14821 cctcgaaacc ttcctgaatg agcagcgtaa cgcacatccg aatcagagcc tgaaaaacac
    14881 actggcggtt catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc
    14941 ggatgtttcg ctaaaacagc tcaacgtgcg tgaccaacag gcactgatta gcacattgac
    15001 cgactggcgc gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg
    15061 cggcgtggac accaacgaac tctcttcacg gacgatggaa gcgcgcaaag tgcctgggct
    15121 gtacttcatc ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg
    15181 ggcgtggtcg agtgcgtggg cttgtgcgca ggatttgatt gcagcgaagt cgtcctgacc
    15241 tgttacctaa agatgaaaat tattagagcc gcattaaaaa tgagctaatt ttgatagtgg
    15301 ctatcttgtg attattttct aatgagcccg tgaactgaaa ccctccaggc ttaatataag
    15361 gtggaaggaa agatgattga aaactcaccc agtggccaga tctttataaa atatgaacag
    15421 gctgaaaaag acagtaactt tgttgttttt tatccttaac cactatgcat taatgctgcg
    15481 ttatttcatg atgcctaaga aaaaccagcg ttacgcaaat ggtcaacgct ggttttatcc
    15541 ggtacgttgc aattattttt tagcagaacc tgcttctaat attgaaacga ttgagaacaa
    15601 cgtaaagcat accgctccca aaccgaccaa aacccgcgaa ggaataaaat atcccatgtc
    15661 ggtctgcgcc atttccgcaa gaaacgatgc caggaaaagg caaaacaggc aggtgaagac
    15721 gggaatcatc ggtattctgt tggctaacga acaggtacgt ctccatacca gtgccagtag
    15781 ccagactttt gagaatatgc tgtaacagat cattccgagg taaataagaa taatgccggg
    15841 agccagtcgg gcgcttgcat cggaactgac taagacgtat atcccctgca gtaccgtgat
    15901 tgagccgaga aaaataaccc aataacacca cagccagtgt tctttagttg aaaacgtatt
    15961 acgtgtttga tggacaattg tggcaacaag gccaattaga caggcacaga ttgcggttaa
    16021 cccaagcaat acgtgacccg cgacataatg cggagttatg tcagcactac gtaacagcgt
    16081 aatagaccaa atgaaaccga gcagggtgag taaaacggga acggcaatga ggcagttacc
    16141 gattaatgaa gaataagcct gtaccggtgt tccgtcgctc ttgctccccg ctgcattttt
    16201 gggaatgagc agaaagtgac cggacgacgc tgccaccgtc gatacacatg cggcaatcat
    16261 accaatgccg aaaataacat agccggcgac aaactcgtct gccatcacat cattgcctgc
    16321 taacagtgcc caaccccata tcatggtgat aattgaccct gcatagccaa taatggggaa
    16381 caatatattg taaaacgtat taacgccgcg cgtgagctgc gaaatgataa taaatgcggt
    16441 agtgaataat gccaggcata tggctgccag agaaatcagc acatgacccg cgacgaaata
    16501 ctcgctttgc cctgaacttt ccagcacaaa gccacctaac gcgatacaaa ttaagcccat
    16561 aatcaaaggg attaatttga aaagccaccc gatatatatg ttcactttaa act
//
LOCUS       DAJVTY010000091        16498 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVTY010000091 DAJVTY010000000
VERSION     DAJVTY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16498
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.091"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(18..2207)
                     /locus_tag="OQQ66_002552"
     CDS             complement(18..2207)
                     /locus_tag="OQQ66_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCS1905440.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2416..2491)
                     /locus_tag="OQQ66_002553"
     tRNA            complement(2416..2491)
                     /locus_tag="OQQ66_002553"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2456..2458),aa:Ala,seq:ggc)
     gene            complement(2531..2606)
                     /locus_tag="OQQ66_002554"
     tRNA            complement(2531..2606)
                     /locus_tag="OQQ66_002554"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2571..2573),aa:Ala,seq:ggc)
     gene            2827..3186
                     /locus_tag="OQQ66_002555"
     CDS             2827..3186
                     /locus_tag="OQQ66_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1905441.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3188..3580
                     /locus_tag="OQQ66_002556"
     CDS             3188..3580
                     /locus_tag="OQQ66_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1905442.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3631..5046)
                     /gene="gltX"
                     /locus_tag="OQQ66_002557"
     CDS             complement(3631..5046)
                     /gene="gltX"
                     /locus_tag="OQQ66_002557"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1905443.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5306..5381
                     /locus_tag="OQQ66_002558"
     tRNA            5306..5381
                     /locus_tag="OQQ66_002558"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5339..5341,aa:Val,seq:tac)
     gene            5426..5501
                     /locus_tag="OQQ66_002559"
     tRNA            5426..5501
                     /locus_tag="OQQ66_002559"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5459..5461,aa:Val,seq:tac)
     gene            5548..5623
                     /locus_tag="OQQ66_002560"
     tRNA            5548..5623
                     /locus_tag="OQQ66_002560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5581..5583,aa:Val,seq:tac)
     gene            5628..5703
                     /locus_tag="OQQ66_002561"
     tRNA            5628..5703
                     /locus_tag="OQQ66_002561"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5661..5663,aa:Lys,seq:ttt)
     gene            5823..6164
                     /locus_tag="OQQ66_002562"
     CDS             5823..6164
                     /locus_tag="OQQ66_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1905444.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6155..7081)
                     /locus_tag="OQQ66_002563"
     CDS             complement(6155..7081)
                     /locus_tag="OQQ66_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1905445.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALLLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7171..8169
                     /locus_tag="OQQ66_002564"
     CDS             7171..8169
                     /locus_tag="OQQ66_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1905446.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8166..8384)
                     /locus_tag="OQQ66_002565"
     CDS             complement(8166..8384)
                     /locus_tag="OQQ66_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1905447.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8386..10401)
                     /gene="ligA"
                     /locus_tag="OQQ66_002566"
     CDS             complement(8386..10401)
                     /gene="ligA"
                     /locus_tag="OQQ66_002566"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1905448.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10472..11458)
                     /gene="zipA"
                     /locus_tag="OQQ66_002567"
     CDS             complement(10472..11458)
                     /gene="zipA"
                     /locus_tag="OQQ66_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1905449.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11688..12449
                     /gene="cysZ"
                     /locus_tag="OQQ66_002568"
     CDS             11688..12449
                     /gene="cysZ"
                     /locus_tag="OQQ66_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1905450.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12634..13605
                     /gene="cysK"
                     /locus_tag="OQQ66_002569"
     CDS             12634..13605
                     /gene="cysK"
                     /locus_tag="OQQ66_002569"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1905451.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            13989..14246
                     /gene="ptsH"
                     /locus_tag="OQQ66_002570"
     CDS             13989..14246
                     /gene="ptsH"
                     /locus_tag="OQQ66_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1905452.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14291..16018
                     /gene="ptsI"
                     /locus_tag="OQQ66_002571"
     CDS             14291..16018
                     /gene="ptsI"
                     /locus_tag="OQQ66_002571"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1905453.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            16059..>16498
                     /gene="crr"
                     /locus_tag="OQQ66_002572"
     CDS             16059..>16498
                     /gene="crr"
                     /locus_tag="OQQ66_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1905454.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDE"
BASE COUNT         4164 a         4244 c         4048 g         4042 t
ORIGIN      
        1 cggttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa atacccttgc
       61 agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc gacaaactcc
      121 gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac aatcatcgca
      181 tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat gatatcagcc
      241 tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc aatcgcaatc
      301 cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac
      361 gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata acgcttaaac
      421 agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat taaattgact
      481 gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc
      541 agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg cagaaactta
      601 tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc ataaccttca
      661 ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc taacgccgtg
      721 cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat ctcctctctg
      781 ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca gcttaactgc
      841 cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc agcaccatag
      901 cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat gttaaccata
      961 tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc actacccggc
     1021 aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca aattgagcgg
     1081 atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag attatcaatg
     1141 cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa cgcacggaag
     1201 ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg attaagccat
     1261 tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga aatcagcacc
     1321 gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt ctggttgtaa
     1381 ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagctta
     1441 ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc aataaagtcg
     1501 ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag ccagcttaag
     1561 gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca caatatctgc
     1621 gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt gtaaatcagc
     1681 acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa
     1741 aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat gctgcatttg
     1801 atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata tcggctggtc
     1861 aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc acacaacacg
     1921 acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg aaagttccac
     1981 agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc aaagataaac
     2041 aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact ggaatggata
     2101 atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag cgtaaacgct
     2161 agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc
     2221 tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc gaagcaaaca
     2281 gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcactaaccc
     2341 ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag
     2401 cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
     2461 gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca aatttgctgg
     2521 gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag
     2581 cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat
     2641 catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga
     2701 aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac
     2761 ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata cattcaggga
     2821 attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc
     2881 cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc
     2941 gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgc
     3001 aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt
     3061 gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca
     3121 tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc
     3181 aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg aatgcctcgg
     3241 tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa cggaaaaatt
     3301 tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt
     3361 tattcagaac acgccagcct tccataacac accaatgttg atggaagctg aagagagaat
     3421 tgctgaatac gctcctggag tacgcgcccc ggcgtatcga caaatcatca acgcgataga
     3481 taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg
     3541 caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg gcaggatatt
     3601 atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt cagcaataaa
     3661 agccagcgct ttgttgatac gctcaatact gcgggtccta ccaattgcgt gaacggtaac
     3721 atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca taccaacttt
     3781 acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa cgttttcagc
     3841 ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct gacgcgctac
     3901 cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt cgtagaaata
     3961 acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag
     4021 atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt gccactgtaa
     4081 gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca gccacagcag
     4141 cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga agtatttgat
     4201 catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac gcaccagata
     4261 gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc tgaccgcccc
     4321 gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt gcgcgtaaac
     4381 cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt tgttgatatg
     4441 gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa ccacacagaa
     4501 gttataggtt ggggaaccat cggtacggcg aataataaga tcgtccagtt cctggttgct
     4561 gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc
     4621 aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc ggcagcgacc
     4681 gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc
     4741 tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt
     4801 gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcca ggctcagcca
     4861 gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc
     4921 ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg agtaaagggc
     4981 agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc gagttttgat
     5041 tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt
     5101 gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg tttctacctt
     5161 acgcattatt gttttcgttg acaaattgcg caacacggtc tattttgcga tcatcttgct
     5221 taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt tgaactctcc
     5281 ctataatgcg actccacaca gcggggggtg attagctcag ctgggagagc acctccctta
     5341 caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta tgtagtctcc
     5401 gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc acctccctta
     5461 caaggagggg gtcggcggtt cgatcccgtc atcacccacc actttctcac cagctaaatt
     5521 tcttgtaaca atgtgaagta ccgaagtggg tgattagctc agctgggaga gcacctccct
     5581 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg tcgttagctc
     5641 agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca
     5701 ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt aagattgatt
     5761 tttcttctca agttccttgt tcttctaccg atattacctc gtacagtgac gaggactgaa
     5821 caatggccac tatctcttca acttcaattc cttccataca aacacagagt agtaacaggg
     5881 catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa atcatcaaac
     5941 tcactcaaca aattaaagag attgtagaca ctagcggcag tgctgaagac aagcaaaaac
     6001 aggccgaatt gattcaacag caaatcaccc tactggaaac acaactggcg cagttgcaga
     6061 aacagcaggc agaaaaggcg caggagaaag agcagcgttt gtctctggat gtatcccttc
     6121 taaatcctgt tgaaaaaacg actcatatcg atatctatat ctgatacaac ggatcccctt
     6181 cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac gcttcagcgg
     6241 cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca acaaccggcg
     6301 taatgcgctt taccaccagt gggctacctt caggcaatag tagcgccagt gcgggaagaa
     6361 tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt ccaatctcct
     6421 gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc ggtcggctgc
     6481 ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag ggtacataat
     6541 cttccacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg gaaagaatcg
     6601 cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag tcgacatctc
     6661 cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca aactgaatat
     6721 ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg gaaatggtct
     6781 ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg gtatcgcgca
     6841 acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc tgccctgcat
     6901 ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta tgattttcca
     6961 gctccttcac actgtgactt actgccgact ggctcaagcc gatacgctct cctgcacgac
     7021 taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa gaataattca
     7081 tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact ggaagaagca
     7141 caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct cgatcctttc
     7201 accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag aggcgatttc
     7261 gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt tatgcacggc
     7321 gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact gcatttgtgg
     7381 gtaatgtgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc ctggtggaaa
     7441 ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcac tctcccggct
     7501 accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc ggcggcggtt
     7561 tgttctgcgt cagcatccag cctgctggga attttccttt caccattgct ggttggtctg
     7621 gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat tatgctgcaa
     7681 ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga ctgggtgtcg
     7741 cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt ggtttataca
     7801 gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg atcattgctg
     7861 tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa cgtctttatg
     7921 gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg tggttcgaaa
     7981 aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc ggtgatcggt
     8041 atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg tgcggtgctg
     8101 gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag cagcgccgat
     8161 aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc ggttttaatc
     8221 agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc tttgcgggca
     8281 aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta tttgccaaac
     8341 ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc tacccagcaa
     8401 acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct tcgccagttt
     8461 agatcctgca gcttcacccg caatcaccag atcggttttc ttcgacacgc tgcccgcgac
     8521 tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct ggcttaagct
     8581 gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg cgttgataac
     8641 gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat tgcggttgct
     8701 ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat caggcacctt
     8761 ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat atgccgccag
     8821 acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga agcgagcaaa
     8881 ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt ttggccccat
     8941 acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg gagtgtggac
     9001 atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa catccatcgc
     9061 acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa tcaggccacc
     9121 ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac ataccggaca
     9181 atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca cgacgttaac
     9241 cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa tgcgtaaacc
     9301 aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca cgccagcaac
     9361 atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc
     9421 gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcta ccgcccagcg
     9481 cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga ccttaatcac
     9541 cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt gatagaacgc
     9601 cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca acccccactt
     9661 tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt ccagaacacc
     9721 aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat caagctgacg
     9781 cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg tgcgtcgcgc
     9841 atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac gcacttccag
     9901 acgcgccggg atattctctc catgcagctt cagcggaatg gcgcgaatag tacgcacatt
     9961 agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga ctaaaacgcc
    10021 attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc accaggtgac
    10081 tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa agctttcttc
    10141 atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc tgaaagccgc
    10201 cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt
    10261 agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg
    10321 cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca gttctgtcag
    10381 ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga caagcggggg
    10441 ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga
    10501 tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga tcgtcaagca
    10561 cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc ttgaagttct
    10621 gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga gtagtgaaat
    10681 ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg aataacgccg
    10741 ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca aaaatgaagc
    10801 ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg tgatgcgccg
    10861 cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact ggagccggtt
    10921 ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa gcctgttgtg
    10981 ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc gaaactggct
    11041 gctgcaacgg ctgttcaggc tgcggctgat agacaggctg ctgcaccggc tgcgctggac
    11101 gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt tgacgcggct
    11161 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    11221 cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
    11281 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    11341 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta
    11401 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    11461 tattctctgt tgttctaaca ccttgccaca acggcaaaca tttactcact aagagtattt
    11521 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    11581 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    11641 atacgcagaa ttttcctagg attggtcaaa aggagctcat cctgattatg gtttcatcat
    11701 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    11761 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    11821 gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactctc atgagttacg
    11881 ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc
    11941 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
    12001 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
    12061 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct
    12121 ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa
    12181 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    12241 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    12301 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc
    12361 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    12421 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    12481 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    12541 tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    12601 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    12661 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    12721 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    12781 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc
    12841 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    12901 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    12961 aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    13021 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    13081 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    13141 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    13201 aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    13261 gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    13321 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    13381 atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt
    13441 gcaggtatct cttctggagc agctgttgcc gcggcgttga aactacaaga agatgaaagc
    13501 tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc
    13561 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    13621 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    13681 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    13741 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    13801 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    13861 aatggactgt attgcgctct tcgtgcgtcg cgtctattaa aaactggcgc taacaataca
    13921 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    13981 gaaatacaat gttccagcaa gaagttacca ttacagctcc gaacggtctg cacacccgcc
    14041 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    14101 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    14161 gtaccgttgt gactatctcc gcagaaggcg aagacgagca aaaagcggtt gaacatctgg
    14221 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    14281 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    14341 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga
    14401 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    14461 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    14521 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca
    14581 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    14641 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    14701 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    14761 tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    14821 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    14881 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    14941 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    15001 taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    15061 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac
    15121 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    15181 tgagttccta ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    15241 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    15301 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    15361 gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcaac tccgcgctat
    15421 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    15481 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    15541 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    15601 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    15661 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    15721 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    15781 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    15841 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    15901 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    15961 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    16021 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    16081 aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt gctccgctct
    16141 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    16201 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca
    16261 ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac
    16321 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    16381 cagaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    16441 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaat
//
LOCUS       DAJVTY010000092        16478 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVTY010000092 DAJVTY010000000
VERSION     DAJVTY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16478
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.092"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(122..1405)
                     /gene="gltA"
                     /locus_tag="OQQ66_002573"
     CDS             complement(122..1405)
                     /gene="gltA"
                     /locus_tag="OQQ66_002573"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1905455.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1611..1718)
                     /locus_tag="OQQ66_002574"
     CDS             complement(1611..1718)
                     /locus_tag="OQQ66_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905456.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2099..2503
                     /gene="sdhC"
                     /locus_tag="OQQ66_002575"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2099..2503
                     /gene="sdhC"
                     /locus_tag="OQQ66_002575"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1905457.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2497..2844
                     /gene="sdhD"
                     /locus_tag="OQQ66_002576"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2497..2844
                     /gene="sdhD"
                     /locus_tag="OQQ66_002576"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1905458.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2844..4610
                     /gene="sdhA"
                     /locus_tag="OQQ66_002577"
     CDS             2844..4610
                     /gene="sdhA"
                     /locus_tag="OQQ66_002577"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1905459.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4626..5342
                     /gene="sdhB"
                     /locus_tag="OQQ66_002578"
     CDS             4626..5342
                     /gene="sdhB"
                     /locus_tag="OQQ66_002578"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1905460.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5643..8444
                     /gene="sucA"
                     /locus_tag="OQQ66_002579"
     CDS             5643..8444
                     /gene="sucA"
                     /locus_tag="OQQ66_002579"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1905461.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYSDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8459..9676
                     /gene="odhB"
                     /locus_tag="OQQ66_002580"
     CDS             8459..9676
                     /gene="odhB"
                     /locus_tag="OQQ66_002580"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1905462.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLITIK
                     ELLEDPTRLLLDV"
     gene            9952..11118
                     /gene="sucC"
                     /locus_tag="OQQ66_002581"
     CDS             9952..11118
                     /gene="sucC"
                     /locus_tag="OQQ66_002581"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1905463.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11118..11987
                     /gene="sucD"
                     /locus_tag="OQQ66_002582"
     CDS             11118..11987
                     /gene="sucD"
                     /locus_tag="OQQ66_002582"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1905464.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12091..12813)
                     /gene="mngR"
                     /locus_tag="OQQ66_002583"
     CDS             complement(12091..12813)
                     /gene="mngR"
                     /locus_tag="OQQ66_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1905465.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12982..14898
                     /gene="mngA"
                     /locus_tag="OQQ66_002584"
     CDS             12982..14898
                     /gene="mngA"
                     /locus_tag="OQQ66_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1905466.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLVGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14916..>16478
                     /locus_tag="OQQ66_002585"
     CDS             14916..>16478
                     /locus_tag="OQQ66_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1905467.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQ"
BASE COUNT         3957 a         4134 c         4486 g         3901 t
ORIGIN      
        1 ttagaatata tgaatgtccg atagtggatt ttatttttac aattttttgt gtttaggcat
       61 ataaaaatta acccgccatt tgaacggcgg gttaaaatat ttacaactta gcaatcaacc
      121 attaacgctt gatatcgctt ttaaagtcgc gtttttcata tcctgtatac agctgacgcg
      181 gacgggcaat cttcataccg tcactgtgca tttcgctcca gtgggcgatc cagccaacgg
      241 tacgtgccat tgcgaaaatg acggtgaaca tggaagacgg aatacccatc gctttcagga
      301 tgataccaga gtagaaatcg acgttcgggt acagtttctt ctcgataaag tacgggtcgt
      361 tcagcgcgat gttttccagc tccatagcca cttccagcag gtcatctttg gtgcccagct
      421 ctttcagaac ttcatggcag gtttcacgca ttacggtggc gcgcgggtcg taatttttgt
      481 acacgcggtg accgaagccc atcaggcgga aagaatcatt tttatctttc gcacgacgaa
      541 caaattccgg aatgtgttta acggagctaa tttcttccag cattttcagc gccgcttcgt
      601 tagcaccacc gtgcgcaggt ccccacagtg aagcaatacc tgctgcgata caggcaaacg
      661 ggttcgcacc cgaagagcca gcggtacgca cggtggaggt agaggcgttc tgttcatggt
      721 cagcgtgcag gatcagaata cggtccatag cacgttccag aatcggatta acttcatacg
      781 gttcgcacgg cgtggagaac atcatattca ggaagttacc ggcataggag agatcgttgc
      841 gcggataaac aaatggctga ccaatggaat acttgtaaca catcgcggcc atggtcggca
      901 ttttcgacag caggcggaac gcggcaattt cacgatgacg aggattgtta acatccagcg
      961 agtcgtgata gaacgccgcc agcgcgccgg taataccaca catgactgcc attggatgtg
     1021 agtcgcgacg gaaagcgtgg aacagacggg taatctgctc gtggatcatg gtatgacggg
     1081 tcaccgtagt tttaaattcg tcatactgtt cctgagtcgg tttttcacca ttcagcagga
     1141 tgtaacaaac ttccaggtag ttagaatcgg tcgccagctg atcgatcggg aaaccgcggt
     1201 gcagcaaaat accttcatca ccatcaataa aagtaatttt agattcgcag gatgcggttg
     1261 aagtgaagcc tgggtcaaag gtgaacacac cttttgaacc gagagtacgg atatcaataa
     1321 catcttgacc cagcgtgcct ttcagcacat ccagttcaac agctgtgtcc ccgttgaggg
     1381 tgagttttgc ttttgtatca gccatttaag gtctccttag cgccttattg cgtaagactg
     1441 ccggaactta aatttgcctt cgcacatcaa cctggcttta cccgtttgtt atttggctcg
     1501 ccgctctgtg aaagagggga aaacctgggt acagagctct gggcgcttgc aggtaaagga
     1561 tccattgatg acgaataaat ggcgaatcaa gtacttagca atccgaatta ttaaacttgt
     1621 ctaccactaa taactgtccc gaatgaattg gtcaatactt ccacactgtt acataagtta
     1681 atcttaggtg aaataccgac ttcataactt ttacgcatta tatgcttttc ctggtaatgt
     1741 ttgtaacaac tttgttgagt gattgtcaaa ttagatgatt aaaaattaaa taaatgttgt
     1801 tgtcgtgacc tggatcactg ttcaggataa aacccgacaa actatatgta ggttaattgt
     1861 aatgattttg tgaacagcct atactgccgc caggtctccg gaacaccctg caatcccgag
     1921 ccacccagcg ttgtaacgtg tcgttttcgc atctggaagc agtgttttgc atgacgcgca
     1981 gttatagaaa ggacgctgtc tgacccgcaa gcagaccgga ggaaggaaat cccgacgtct
     2041 ccaggtaaca gaaagttaac ctctgtgccc gtagtcccca gggaataata agaacagcat
     2101 gtgggcgtta ttcatgataa gaaatgtgaa aaaacaaaga cctgttaatc tggacctaca
     2161 gaccatccgg ttccccatca cggcgatagc gtccattctc catcgcgttt ccggtgtgat
     2221 cacctttgtt gcagtgggca tcctgctgtg gcttctgggt accagcctct cttcccctga
     2281 aggtttcgag caagcttccg cgattatggg cagcttcttc gtcaaattta tcatgtgggg
     2341 catccttacc gctctggcgt atcacgtcgt cgtaggtatt cgccacatga tgatggattt
     2401 tggctatctg gaagaaacat tcgaagcggg taaacgctcc gccaaaatct cctttgttat
     2461 tactgtcgtg ctttcacttc tcgcaggagt cctcgtatgg taagcaacgc ctccgcatta
     2521 ggacgcaatg gcgtacatga tttcatcctc gttcgcgcta ccgctatcgt cctgacgctc
     2581 tacatcattt atatggtcgg ttttttcgcc accagtggcg aactgacata tgaagtctgg
     2641 atcggtttct tcgcctctgc gttcaccaaa gtgttcaccc tgctggcgct gttttctatc
     2701 ttgattcatg cctggatcgg catgtggcag gtgttgaccg actacgttaa accgctggca
     2761 ttgcgcctga tgctgcaact ggtgattgtc gttgcactgg tggtttacgt gatttatgga
     2821 ttcgttgtgg tgtggggtgt gtgatgaaat tgccagtcag agaatttgat gcagttgtga
     2881 ttggtgccgg tggcgcaggt atgcgcgcgg cgctgcaaat ttcccagagc ggccagacct
     2941 gtgcgctgct ctctaaagtc ttcccgaccc gttcccatac cgtttctgcg caaggcggca
     3001 ttaccgttgc gctgggtaat acccatgaag ataactggga atggcatatg tacgacaccg
     3061 taaaagggtc ggactatatc ggtgaccagg acgcgattga atatatgtgt aaaaccgggc
     3121 cggaagcgat tctggaactc gaacacatgg gcctgccgtt ctcgcgtctt gatgatggtc
     3181 gtatctatca acgtccgttt ggcggtcagt cgaaaaactt cggcggcgag caggcggcac
     3241 gcactgcggc ggcggctgac cgtaccggtc acgcactgtt gcacacgctt tatcagcaga
     3301 acctgaaaaa ccacaccacc attttctccg agtggtatgc gctggatctg gtgaagaacc
     3361 aggatggcgc ggtggtgggt tgtactgcac tgtgcatcga aactggtgaa gtggtttatt
     3421 tcaaagcccg cgctaccgtg ctggcgactg gcggggcagg gcgtatttat cagtccacca
     3481 ctaacgccca cattaacact ggcgacggcg tcggcatggc tatccgcgca ggcgtaccgg
     3541 tgcaggatat ggaaatgtgg cagttccacc cgaccggtat tgccggtgcg ggcgtactgg
     3601 tcaccgaagg ttgccgtggt gaaggcggtt atctgctgaa caaacatggc gaacgcttta
     3661 tggagcgtta tgcgccgaac gccaaagacc tggcgggccg tgatgtggtg gcgcgttcca
     3721 tcatgatcga aatccgtgaa ggtcgcggct gtgatggtcc gtgggggcca cacgcgaaac
     3781 tgaaactcga ccacctgggt aaagaagttc tcgaatcccg tctgccgggc atcctggaac
     3841 tctcccgtac cttcgctcac gtcgatccgg tgaaagagcc gatcccggtt atcccaacct
     3901 gtcattacat gatgggcggt attccgacca aagtgaccgg tcaggcgctg actgtgaatg
     3961 agaaaggcga agatgtggtt gttccgggac tgtttgccgt tggtgaaatc gcttgtgtat
     4021 cggtacacgg cgctaaccgt ctgggcggca actcgctgct ggacctggtg gtctttggtc
     4081 gtgcggcagg tctgcatctg caagagtcta tcgccgagca gggcgcattg cgcgatgcca
     4141 gcgagtctga tgttgaagca tctctggatc gtctgaaccg ctggaacaat aatcgtaacg
     4201 gtgaagatcc ggtggcgatt cgtaaagcgc tgcaagaatg tatgcagcat aacttctcgg
     4261 tcttccgtga aggtgatgcg atggcgaaag ggcttgagca gttgaaagtt atccgcgagc
     4321 gtctgaaaaa tgcccgtctg gatgacactt ccagcgagtt caacacccag cgcgttgagt
     4381 gcctggaact ggataacctg atggaaacgg cgtatgcaac ggctgtttct gccaacttcc
     4441 gtaccgaaag ccgtggcgcg catagccgct tcgacttccc ggatcgtgat gatgaaaact
     4501 ggctgtgcca ctccctgtat ctgccagagt cggaatccat gacgcgccga agcgtcaaca
     4561 tggaaccgaa actgcgcccg gcattcccgc cgaagattcg tacttactaa tgcggagaca
     4621 ggaaaatgag actcgagttt tcaatttatc gctataaccc ggatgttgat gatgctccgc
     4681 gtatgcagga ttacaccctg gaagcggaag aaggtcgcga catgatgctg ctggatgcgc
     4741 ttatccagct gaaagagaaa gatcccagcc tgtcgttccg ccgctcctgt cgtgaaggtg
     4801 tgtgcggttc cgacggtctg aacatgaacg gcaagaatgg tctggcctgt attaccccga
     4861 tttcggcact caaccagccg ggcaagaaga ttgtgattcg cccgctgcca ggtttaccgg
     4921 tgatccgcga tttggtggta gacatgggac aattctatgc gcaatatgag aaaattaagc
     4981 cttacctgtt gaataatgga caaaatccgc cagctcgcga gcatttacag atgccagagc
     5041 agcgcgaaaa actcgacggg ctgtatgaat gtattctctg cgcatgttgt tcaacctctt
     5101 gtccgtcttt ctggtggaat cccgataagt ttatcggccc ggcaggcttg ttagcggcat
     5161 atcgtttcct gatcgatagc cgtgataccg agactgacag ccgcctcgac ggtttgagcg
     5221 atgcattcag cgtattccgc tgtcacagca tcatgaactg cgtcagtgta tgtccgaagg
     5281 ggctgaaccc gacgcgcgcc atcggccata tcaagtcgat gttgttgcaa cgtaatgcgt
     5341 aaaccgtagg cctgataaga cgcgcaagcg tcgcatcagg caaccagtgc cggatgcggc
     5401 gtgaacgcct tatccggcct acaagtcatt acccgtaggc ctgataagcg cagcgcatca
     5461 ggcgtaacaa agaaatgcag gaaatcttta aaaactgccc ctgacactaa gacagttttt
     5521 aaaggttcct tcgcgagcca ctacgtagac aagagctcgc aagtgaaccc cggcacgcac
     5581 atcactgtgc gtggtagtat ccacggcgaa gtaagcataa aaaagatgct taagggatca
     5641 cgatgcagaa cagcgctttg aaagcctggt tggactcttc ttacctctct ggcgcaaacc
     5701 agagctggat agaacagctc tatgaagact tcttaaccga tcctgactcg gttgacgcta
     5761 actggcgttc gacgttccag cagttacctg gtacgggagt caaaccggat caattccact
     5821 ctcaaacgcg tgaatatttc cgccgcctgg cgaaagacgc ttcacgttac tcttcaacga
     5881 tctccgaccc tgacaccaat gtgaagcagg ttaaagtcct gcagctcatt aacgcatacc
     5941 gcttccgtgg tcaccagcat gcgaatctcg atccgctggg actgtggcag caagataaag
     6001 tggccgatct ggatccgtct ttccacgatc tgaccgaagc agacttccag gaaaccttca
     6061 acgtcggttc atttgccagc ggcaaagaaa ccatgaaact cggcgagctg ctggaagccc
     6121 tcaagcaaac ctactgcggc ccgattggtg ccgagtatat gcacatcacc agcaccgaag
     6181 aaaaacgctg gatccaacag cgtatcgagt ctggtcgcgc gactttcaat agcgaagaga
     6241 aaaaacgctt cttaagcgaa ctgaccgccg ctgaaggcct tgaacgttac ctcggcgcaa
     6301 aattccctgg cgcaaaacgc ttctcgctgg aaggcggtga cgcgttaatc ccgatgctta
     6361 aagagatgat ccgccacgct ggcaacagcg gcacccgcga agtggttctc gggatggcgc
     6421 accgtggtcg tctgaacgtg ctggtgaacg tgctgggtaa aaaaccgcaa gacttgttcg
     6481 acgagttcgc cggtaaacat aaagaacacc tcggcacggg tgacgtgaaa taccacatgg
     6541 gcttctcgtc tgacttccag accgatggcg gcctggtgca cctggcgctg gcgtttaacc
     6601 cgtctcacct tgagattgta agcccggtag ttatcggttc tgttcgtgcc cgtctggaca
     6661 gacttgatga gccgagcagc aacaaagtgc tgccaatcac catccacggt gacgccgcag
     6721 tgaccgggca gggcgtggtt caggaaaccc tgaacatgtc gaaagcgcgt ggttatgaag
     6781 ttggcggtac ggtacgtatc gttatcaaca accaggttgg tttcaccacc tctaatccgc
     6841 tggatgcccg ttctacgccg tactgtactg atatcggtaa gatggttcag gccccgattt
     6901 tccacgttaa cgcggacgat ccggaagccg ttgcctttgt gacccgtctg gcgctcgatt
     6961 tccgtaacac ctttaaacgt gatgtcttca tcgacctggt gtgctaccgc cgtcacggcc
     7021 acaacgaagc cgacgagccg agcgcaaccc agccgctgat gtatcagaaa atcaaaaaac
     7081 atccgacacc gcgcaaaatc tacgctgaca agctggagca ggaaaaagtg gcgacgctgg
     7141 aagatgccac cgagatggtt aacctgtacc gcgatgctct ggatgctggc gattgcgtag
     7201 tggcagagtg gcgtccgatg aacatgcact ctttcacctg gtcgccgtac ctcaaccacg
     7261 aatgggacga agagtacccg aacaaagttg agatgaagcg cctgcaggag ctggcgaaac
     7321 gcatcagcac ggtgccggaa gcagttgaaa tgcagtctcg cgttgccaag atttatagcg
     7381 atcgccaggc gatggctgcc ggtgagaaac tgttcgactg gggcggtgcg gaaaacctcg
     7441 cttacgccac gctggttgac gaaggcattc cggttcgcct gtcgggtgaa gactccggtc
     7501 gcggtacctt cttccaccgc cacgcggtga tccacaacca gtctaacggt tccacttaca
     7561 cgccgctgca acacatccat aacgggcagg gcgcgttccg cgtctgggac tccgtactgt
     7621 ctgaagaagc agtgctggcg tttgaatacg gttatgccac cgcagaacca cgcactctga
     7681 ccatctggga agcgcagttc ggtgacttcg ctaacggtgc tcaggtggtt atcgaccagt
     7741 tcatctcctc tggcgaacag aaatggggcc ggatgtgtgg tctggtgatg ttgctgccgc
     7801 acggttatga agggcagggg ccggagcact cctccgcgcg tctggaacgt tatctgcaac
     7861 tttgtgctga gcaaaacatg caggtgtgcg taccgtctac cccggcacag gtttaccaca
     7921 tgctgcgtcg tcaggcgctg cgcgggatgc gtcgtccgct ggtcgtgatg tcgccgaaat
     7981 ccctgctgcg tcatccgctg gcggtttcca gcctcgaaga actggcgaac ggcaccttcc
     8041 tgccagccat cggtgaaatc gacgagcttg atccgaaggg cgtgaagcgc gtagtgatgt
     8101 gttctggtaa ggtttattac gacctgctgg aacagcgtcg taagaacaat caacacgatg
     8161 tcgccattgt gcgtatcgag caactctacc cgttcccgca taaagcgatg caggaagtgt
     8221 tgcagcagtt tgctcacgtc aaggattttg tctggtgcca ggaagagccg ctcaaccagg
     8281 gcgcatggta ctgcagccag catcatttcc gtgaagtgat tccgtttggg gcttctctgc
     8341 gttatgcagg ccgcccggcc tccgcctctc cggcggtagg gtatatgtcc gttcaccaga
     8401 aacagcaaca agatctggtt aatgacgcgc tgaacgtcga ataaataaag gatacacaat
     8461 gagtagcgta gatattctgg tccctgacct gcctgaatcc gtagccgatg ccaccgtcgc
     8521 aacctggcat aaaaaacccg gcgacgcagt cgtacgtgat gaagtgctgg tagaaatcga
     8581 aactgacaaa gtggtactgg aagtaccggc atcagcagac ggcattctgg atgcggttct
     8641 ggaagatgaa ggtacaaccg taacgtctcg tcagatcctt ggtcgcctgc gtgaaggcaa
     8701 cagcgccggt aaagaaacca gcgccaaatc tgaagagaaa gcgtccactc cggcgcaacg
     8761 ccagcaggcg tctctggaag agcaaaacaa cgatgcgtta agcccggcga tccgtcgctt
     8821 gctggctgaa cataacctcg acgccagcgc tattaaaggc accggcgtgg gtggtcgtct
     8881 gacccgtgaa gatgtggaaa aacatctggc gaaatccccg gcgaaagagt ctgcaccggc
     8941 agcggctgct ccggcggcgc aaccggctct ggctgcacgt agtgaaaaac gtgtcccgat
     9001 gactcgcctg cgtaagcgtg tggcagagcg tctgctggaa gcgaaaaact ccaccgccat
     9061 gctgaccacg ttcaacgaag tcaacatgaa gccgattatg gatctgcgta agcagtacgg
     9121 tgaagcgttt gaaaaacgcc acggcatccg tctgggcttt atgtccttct acgtgaaagc
     9181 ggtggttgaa gccctgaaac gttacccgga agtgaatgct tctatcgacg gcgatgacgt
     9241 ggtttaccac aactatttcg acgtcagcat ggcggtttct acgccgcgcg gcctggtgac
     9301 gccggttctg cgtgatgtcg ataccctcgg catggcagac atcgagaaga aaatcaaaga
     9361 gctggcagtc aaaggccgtg atggcaagct gaccgttgaa gatctgaccg gtggtaactt
     9421 caccatcacc aacggtggtg tgttcggttc cctgatgtct acgccgatca tcaacccgcc
     9481 gcagagcgca attctgggta tgcacgctat caaagatcgt ccgatggcgg tgaatggtca
     9541 ggttgagatc ctgccgatga tgtacctggc gctgtcctac gatcaccgtc tgatcgatgg
     9601 tcgcgaatcc gtgggcttcc tgataacgat caaagagttg ctggaagatc cgacgcgtct
     9661 gctgctggac gtgtagtagt ttaagtttca cctgcactgt agacaggata aggcattatc
     9721 gccttctccg gcaattgaag cctgatgcga cgcttaacgc gtcttatcag gcctacggga
     9781 agcaccaatg taggtcggat aaggcgcttg cgccgcatcc gacaagcgat gcctgatgcg
     9841 acacttacgc gtcttatcag gcctacaggt gaccgacaat gcccggaggc gatacgaaat
     9901 attcggtcta cggtttaaaa gataacgatt actgaaggat ggacagaaca catgaactta
     9961 catgaatatc aggcaaaaca actttttgcc cgctatggct taccagcacc ggtgggttat
    10021 gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa aaatcggtgc cggtccgtgg
    10081 gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta
    10141 aacagcaaag aagacatccg tgcttttgca gaaaattggc tcggcaagcg tctggtaacg
    10201 tatcaaacag atgccaatgg ccaaccggtt aaccagattc tggttgaagc agcgaccgat
    10261 atcgctaaag agctgtatct cggtgccgtt gtagaccgta gttcccgtcg tgtggtcttt
    10321 atggcctcca ctgaaggcgg cgtggaaatc gaaaaagtgg cggaagaaac tccgcagctg
    10381 atccataaag ttgcgcttga tccgctgaca ggcccgatgc cgtatcaggg acgcgagctg
    10441 gcgttcaaac tgggtctgga aggtaaactg gttcagcagt tcaccaaaat cttcatgggt
    10501 ctggcgacca ttttcctgga gcgcgacctg gcgttgatcg aaatcaaccc gctggtcatc
    10561 accaaacagg gtgatctgat ttgcctcgac ggcaaactgg gcgctgatgg caacgcactg
    10621 ttccgccagc ttgatctgcg cgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca
    10681 caggctgcac agtgggaact gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt
    10741 aacggcgcag gtctggcgat gggtacgatg gacatcgtta aactgcacgg cggcgaaccg
    10801 gctaacttcc ttgacgttgg cggcggtgca accaaagaac gtgtaaccga agcgttcaaa
    10861 atcatcctct ctgacgacaa agtgaaagtc gttctggtta acatcttcgg cggtatcgtt
    10921 cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag cagaagtggg tgttaacgta
    10981 ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg gcgcgaagaa actggctgac
    11041 agcggcctga atattattgc agcaaaaggt ctgacggatg cagctcagca ggttgttgcc
    11101 gcagtggagg ggaaataatg tccattttaa ttgataaaaa caccaaggtt atctgccagg
    11161 gctttactgg tagccagggg actttccact cagaacaggc cattgcatac ggcactaaaa
    11221 tggttggcgg cgtaacccca ggtaaaggcg gtactaccca cctcggcctg ccggtgttca
    11281 acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc ttctgttatc tacgtaccag
    11341 caccgttctg caaagactcc attctggaag ctattgatgc aggcatcaaa ctgattatca
    11401 ccatcactga aggcatcccg acgctggata tgctgactgt gaaagtgaaa ctggacgaag
    11461 ccggcgttcg tatgatcggc ccgaactgcc cgggcgttat cactccgggt gaatgcaaaa
    11521 tcggtatcca gccgggtcac attcacaaac cgggtaaagt gggtatcgtt tcccgttccg
    11581 gtacactgac ctatgaagcg gttaaacaga ccacggatta cggtttcggt cagtcgacct
    11641 gtgttggtat cggcggtgac ccgatcccag gctctaactt tatcgacatc ctcgaaatgt
    11701 tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg tgagatcggc ggtagcgctg
    11761 aagaagaagc ggcagcgtac atcaaagagc acgttaccaa gccagttgta ggttacatcg
    11821 ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc gggtgcgatc attgccggtg
    11881 ggaaagggac tgcggatgag aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc
    11941 gcagcctggc ggatatcggt gaagcactga aaactgttct gaaataaata tctgtaataa
    12001 gaaatagccc tcgccgcttc cctctacagg aatggcgaag ggctgtcggt ttcgacatgg
    12061 ttggccatcg tatgatggcc ttttttgtgc ttatcgcgat gattttcgct gcgctatcag
    12121 ggtaaattta tagtcatcgg tattaaaagc gttgcggcta tattcaaaca cccgaccatc
    12181 aactaaatat ccacgcgata ctttttcaag aatcggcttt gtctggctga tattaagcag
    12241 acggctcatc tcttcggttg gcatcagagg aatgatttcc tgttcgctac gatcgataac
    12301 catcttcttc acttcttcaa taaagtgata tttcgaattt tccatgacct gccaggtgag
    12361 atccgggaac aacgcaagcg gcatccaggt ttcttccagc gccattggct tttgcttgcg
    12421 atagcgcacg cgcttcacat gccacacacg atcctgcgcg gtgatttgta gctgttgctg
    12481 aagaaaatcg tcagccggaa tcacttcaaa tatcaggact tcactgtgtg tatcgacgtg
    12541 acggtccgac agtttttcat caaaactggt taactgaaaa atatcgtaat tgacccgctc
    12601 ttctttgacg taagtcccgc tgccctgaat gctttcgagg atctgctgct cgactagctg
    12661 gcgcaaagcc tgacgcaccg taacccggct gacgccaaac tctgtttgta gcgctgattc
    12721 agtgggtaac gcatcgccag gtttaagctc gccacgcgca atttgttcac gaatgcgatc
    12781 ggcaatctgc cggtataagg gcttgtgtcc catttttagt atctcattaa tacgaattta
    12841 gccattatgc ccgataaatt catcctgtaa ataatgcaaa tacaatacaa ataatttcaa
    12901 tcaagtgaaa ttgatcatat aatggtattg ttttatcggg cacactggcg cgactataaa
    12961 aacgatcaag tgaggatcat gatgaatctg acgactctga cccaccgcga tgcgttgcgt
    13021 ctgaatgcgc gctttaccag ccgtgaagag gccatccacg cgttgactca acgtcttgct
    13081 gctctgggga aaatttccag tactgagcaa tttctggaag aagtgtatcg ccgtgaaagc
    13141 cttggcccga ccgccttagg tgaagggttg gctgtgccgc atggcaaaac tgctgcggta
    13201 aaagaagcgg cgtttgcggt cgccacactc agcgagccgc ttcagtggga aggcgttgat
    13261 ggcccggaag cagttgattt agtggtgctg ctggcgattc cccccaatga agcgggtact
    13321 atgcatatgc aactgctgac agcgctgacc acgcgccttg cggatgatga gattcgggcg
    13381 cgtatacagt cggcgacgac gcctgatgag ttgctctcgg cgctggatga caagggaggc
    13441 acgcaacctt ctgcctcttt ttccaacgcg ccaactatcg tctgcgtacc ggcctgtccg
    13501 gcgggtattg ctcacaccta tatggctgcg gaatatctgg aaaaagccgg acgcaaactc
    13561 ggcgtaaatg tttacgttga aaaacaaggc gctaacggca ttgaagggcg tttaacggcg
    13621 gatcaactca atagtgcaac cgcctgtatt tttgcggctg aagtcgccat caaggagagt
    13681 gagcgtttta atggcattcc cgcgctttca gtgcctgttg ccgagccgat tcgccatgca
    13741 gaagcgttga tccaacaagc gcttaccctc aagcgtagcg atgagacgcg taccgtacag
    13801 caagatacgc aaccggtgaa aagtgtcaaa acggagctga aacaggcact gttgagcgga
    13861 atctcttttg ccgtaccgtt gattgtcgcg gggggcacgg tgctggcggt cgcggtatta
    13921 ctgtcgcaaa tcttcgggct acaagatctg tttaatgaag aaaactcctg gctgtggatg
    13981 taccgcaagc tgggcggcgg gctgctcgga attttgatgg taccggtgct cgcggcctat
    14041 accgcctatt ctctggcaga taaaccggcg ttagcgccag gctttgcggc tggacttgcc
    14101 gccaacatga tcggctccgg gtttctcggc gcggtcgttg gcggattgat agccggttac
    14161 ttgatgcgct gggtgaaaaa tcacttgcgt cttagcagta aattcaatgg attcctgact
    14221 ttttatctct acccggtgct cggtacgttg ggagcgggca gtctgatgct gtttgtggtg
    14281 ggagaacctg tcgcatggat caataactcg cttaccgcct ggctgaacgg tctgtcagga
    14341 agtaacgcgc tgttgctggg tgccattttc ggttttatgt gttcctttga tcttggaggg
    14401 ccagtgaaca aagccgctta tgcattctgc ctgggcgcaa tggtgaacgg cgtttacggc
    14461 ccgtatgcca ttttcgcctc cgtcaaaatg gtttcggcat ttaccgtaac cgcttccacg
    14521 atgctcgcac cgcgcctgtt taaagagttt gaaattgaga ccgggaaatc cacctggctg
    14581 ttagggctgg taggtattac cgaaggggcg atcccgatgg cgattgaaga tccgctgcgg
    14641 gttattggtt cgtttgtgct gggctctatg gtaacgggcg ctattgtcgg tgcgatgaat
    14701 atcggccttt cgacacccgg tgccggcatt ttctcgctct ttttacttca tgataatggc
    14761 gcgggcggtg ttatggcggc aattggctgg tttggtgcgg cattggtggg ggctgcaatc
    14821 tcgactgcaa ttctcctgat ctggcggcgt cacgcggtta agcatggcaa ctatctgact
    14881 gatggcgtaa tgccataaac aaaaaggaaa cgacgatgaa agcggtatct cgcgttcaca
    14941 tcaccccgca tatgcactgg gatcgagagt ggtatttcac caccgaagag tcacgtattc
    15001 tgctggtcaa taatatggaa gagatcctgt gccgactgga acaggacaac gaatacaaat
    15061 attacgtact cgacgggcaa acggcgatcc tcgaagatta tttcgcggtg aaaccggaaa
    15121 acaaagaccg tgtgaagaaa caggtagaag ccggcaagtt gattatcggc ccctggtata
    15181 cccagaccga taccacgatt gtttctgcgg aatccatcgt ccgtaatctg atgtacggaa
    15241 tgcgtgactg cctcgcgttt ggcgagccga tgaaaatagg ttatttacca gattcctttg
    15301 gcatgtccgg gcaactgccg catatctaca atggatttgg cattacccgc accatgttct
    15361 ggcgcggatg ttcggagcgc cacggtactg ataaaaccga gtttttgtgg caaagcagtg
    15421 acggtagcga agtgacggcg caggtgctgc cgctgggcta cgccataggt aagtacttac
    15481 ctaccgacga aaacggatta cgtaaacgcc tcgacagtta ttttgacgtg ctggaaaaag
    15541 cgtctgtaac caaagagatt ttgctgccga atggtcatga ccagatgcca ttgcagcaaa
    15601 atatcttcga agtgatggat aagctacgtg agatctaccc tcaacgtaag tttgtgatga
    15661 gccgctttga agaggtattt gagaagatcg aagcgcagcg tgaaagtctg gccaccctga
    15721 aaggtgaatt tatcgatggc aaatatatgc gcgtacatcg caccatcggt tccacgcgca
    15781 tggatatcaa aattgcccac gcacgtattg aaaataagat tgttaatctg ctggaaccgc
    15841 tggcaacgct ggcctggacg ttgggttttg aataccacca cggcttgctg gagaaaatgt
    15901 ggaaagagat cttaaaaaat catgcccacg acagtatcgg ttgttgttgc agtgacaaag
    15961 ttcatcgcga aattgtcgcc cgcttcgaac tggctgaaga catggcggat aatctgctgc
    16021 gtttctacat gcgcaaaatt gccgataaca tgccacagag tgatgccgac aaactcgtgc
    16081 tgtttaacct gatgccctgg ccgcgggaag aagttattaa caccactgtg cggctgcgcg
    16141 ccagccagtt taatctacga gacgatcgcg gtcagcctgt accgtatttt attcgccatg
    16201 cccgtgagat cgatccaggc ctaatcgatc ggcaaatagt tcattacggt aattacgatc
    16261 cctttatgga gtttgatata cagatcaacc agattgtccc ttctatgggc tatcgcacgc
    16321 tttatatcga agcgaatcag cctggcaacg tgattgcggc aaaaagtgac gctgaaggga
    16381 tactggaaaa tgctttctgg caaattgcgc tcaatgagga tggttctctg caactggtag
    16441 ataaagacag cggtgtgcgc tatgaccgag tattgcaa
//
LOCUS       DAJVTY010000093        16014 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVTY010000093 DAJVTY010000000
VERSION     DAJVTY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16014
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.093"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..190
                     /locus_tag="OQQ66_002586"
     CDS             <1..190
                     /locus_tag="OQQ66_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905468.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OQQ66_002587"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OQQ66_002587"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1905469.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OQQ66_002588"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OQQ66_002588"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1905470.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OQQ66_002589"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OQQ66_002589"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1905471.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OQQ66_002590"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OQQ66_002590"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1905472.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OQQ66_002591"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OQQ66_002591"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1905473.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OQQ66_002592"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OQQ66_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1905474.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OQQ66_002593"
     CDS             7144..7353
                     /locus_tag="OQQ66_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1905475.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OQQ66_002594"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OQQ66_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1905476.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..9213
                     /locus_tag="OQQ66_002595"
                     /pseudo
     CDS             8228..9213
                     /locus_tag="OQQ66_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            9576..10613
                     /gene="dusA"
                     /locus_tag="OQQ66_002596"
     CDS             9576..10613
                     /gene="dusA"
                     /locus_tag="OQQ66_002596"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1905477.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10747..10989
                     /gene="pspG"
                     /locus_tag="OQQ66_002597"
     CDS             10747..10989
                     /gene="pspG"
                     /locus_tag="OQQ66_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1905478.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11154..12137)
                     /locus_tag="OQQ66_002598"
     CDS             complement(11154..12137)
                     /locus_tag="OQQ66_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1905479.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12220..13635
                     /gene="dnaB"
                     /locus_tag="OQQ66_002599"
     CDS             12220..13635
                     /gene="dnaB"
                     /locus_tag="OQQ66_002599"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1905480.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13688..14767
                     /gene="alr"
                     /locus_tag="OQQ66_002600"
     CDS             13688..14767
                     /gene="alr"
                     /locus_tag="OQQ66_002600"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1905481.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15020..>16014
                     /gene="tyrB"
                     /locus_tag="OQQ66_002601"
     CDS             15020..>16014
                     /gene="tyrB"
                     /locus_tag="OQQ66_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1905482.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLST"
BASE COUNT         3826 a         3833 c         4245 g         4110 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacag cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat
     8641 gcgactccca ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg
     8701 gttcagctat tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa
     8761 acaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc
     8821 ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta
     8881 tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa
     8941 ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc tgatggggga
     9001 gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga agggacgctc
     9061 gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag
     9121 tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt
     9181 tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa
     9241 ggcattatat aaaaggaccc aatatttatt gggtcctttt ttctctatca atgctattag
     9301 cagggagata tatcaccaaa gtttaatgtg tgatttttta tttatcgtcg aacctggatt
     9361 gtttatcatt ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag
     9421 agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg
     9481 ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata acataacccc
     9541 atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa
     9601 aaatcaacca aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta
     9661 gcgttgcacc aatgctcgac tggacggaca gacattgccg ctatttcttg cgtctgcttt
     9721 cccgcaatac gttgctgtat accgaaatgg tgaccacagg ggcgattatt cacggtaaag
     9781 gtgattacct ggcgtacagt gaagaagaac atccggtagc gttgcaactc ggcggtagcg
     9841 atccggcggc gctggcacag tgtgcgaagc tggcagaagc gcgtggatat gatgagatca
     9901 acctgaatgt cggctgcccg tctgaccggg tgcagaacgg catgtttggt gcgtgtctga
     9961 tgggtaatgc gcagctggtt gccgattgcg tgaaagcgat gcgcgatgtg gtgtcgattc
    10021 cggtgacggt gaaaacgcgt attggcatcg atgaccagga cagctatgaa tttctctgcg
    10081 atttcatcaa caccgtttcc ggcaaaggcg agtgtgggat gttcatcatc catgcacgta
    10141 aagcctggct ttcggggtta agcccgaaag aaaaccgtga aatcccgccg ctcgattatc
    10201 cgcgtgtgta tcaactgaag cgtgactttc cgcatctgac aatgtcgatt aacggtggta
    10261 tcaagtcgct ggaagaggcc aaagcacacc tgcaacatat ggatggcgtg atggtcgggc
    10321 gcgaggcgta tcagaatccg ggtattctgg cggcggtaga ccgggagatc tttggttcct
    10381 cggataccga tgcctatccg gtggcggtag tgcgcgccat gtatccgtac attgagcgtg
    10441 aactcagcca ggggacgtat ctcggccata ttacccggca tatgttgggc ttgttccagg
    10501 gtattcctgg cgcgcggcag tggcggcgtt atttaagtga aaatgcccat aaagcgggtg
    10561 cagacattaa tgtgctggaa cacgcgctca aactggtggc ggataagcgt taacttttca
    10621 ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa
    10681 tcaatagatt atttttggca tgattcttgt aatgccagca agagatttca tatttgggag
    10741 agcatcatgc tggaactact ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc
    10801 gtttcgttgc tgggcattat cgccgcgctg gttgtggcga cggccattat gttcctcggc
    10861 ggtatgctgg cattgatgat taagttgctg ccgtggttac tactggcgat tgcggtggtg
    10921 tgggttatta aggcgattaa agcaccaaaa gtgcctaaat atcagcgtta tgaccgctgg
    10981 cgttactaag ggattgtgcg gatgatcaca acctaaggtt ttatccttag aacaaaatag
    11041 gaattgataa tcaaatctgt cactattgcg cctctaacag attcatcgtg ctgtacccta
    11101 catacagccg actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
    11161 atcagcaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
    11221 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
    11281 aaagagaaca gttcattact ggcctcggtt aattcctccc gtgtggtgat atagccttgc
    11341 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
    11401 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
    11461 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ctacgcgcac tttcttaccg
    11521 ccggtgatct ctttcaatcg ctcgaccaga ttctcttcac gatagttaat aacctgccac
    11581 gcgccggctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
    11641 gcgcccagag cttttgccca ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg
    11701 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
    11761 aagcctttca ggaaagatgc tgcagcttgc tcaaaagaaa tagccgcagg cagaatcgcc
    11821 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
    11881 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
    11941 gccgcttcgg tacctgatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
    12001 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
    12061 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
    12121 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
    12181 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
    12241 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
    12301 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
    12361 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
    12421 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
    12481 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
    12541 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
    12601 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
    12661 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa
    12721 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
    12781 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
    12841 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
    12901 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
    12961 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
    13021 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
    13081 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
    13141 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
    13201 cgccgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
    13261 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
    13321 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
    13381 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
    13441 atcgagcagg atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac
    13501 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
    13561 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
    13621 tacgacgacg aataataatt attttatgaa ttaggcaatt aaagcaaaca cttatcaagg
    13681 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
    13741 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
    13801 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
    13861 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
    13921 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
    13981 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
    14041 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctgg
    14101 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
    14161 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
    14221 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
    14281 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
    14341 gccgctggaa gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac
    14401 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
    14461 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
    14521 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
    14581 cgggcgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
    14641 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
    14701 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
    14761 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
    14821 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
    14881 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
    14941 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
    15001 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
    15061 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
    15121 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
    15181 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
    15241 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
    15301 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
    15361 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
    15421 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
    15481 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
    15541 tattgtgttg ctgcatccat gttgccacaa cccaacaggt gccgatctca ctaatgatca
    15601 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
    15661 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
    15721 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
    15781 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
    15841 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaatttgg gtgcgcaggt
    15901 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
    15961 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcac
//
LOCUS       DAJVTY010000094        15938 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVTY010000094 DAJVTY010000000
VERSION     DAJVTY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.094"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1737
                     /gene="crfC"
                     /locus_tag="OQQ66_002602"
     CDS             <1..1737
                     /gene="crfC"
                     /locus_tag="OQQ66_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1905483.1"
                     /translation="ENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAA
                     IEHIPVIEVEFVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVL
                     DYTQLKSISDEEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGC
                     ITPQQIFPVSSMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLAD
                     LEHIRHAADQLWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFR
                     AHGLNVACEQLRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAL
                     NAQLAALFQDDSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRP
                     IEQQVKMELSESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRE
                     TFSRWLNQPDWGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAG
                     MNTFFAEFASCLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLR
                     DDIQTLFTAERY"
     gene            1734..2617
                     /locus_tag="OQQ66_002603"
                     /pseudo
     CDS             1734..2617
                     /locus_tag="OQQ66_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            2881..4383
                     /gene="proP"
                     /locus_tag="OQQ66_002604"
     CDS             2881..4383
                     /gene="proP"
                     /locus_tag="OQQ66_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1905484.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4449..4586
                     /gene="pmrR"
                     /locus_tag="OQQ66_002605"
     CDS             4449..4586
                     /gene="pmrR"
                     /locus_tag="OQQ66_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1905485.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(4604..5662)
                     /gene="pmrB"
                     /locus_tag="OQQ66_002606"
     CDS             complement(4604..5662)
                     /gene="pmrB"
                     /locus_tag="OQQ66_002606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1905486.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRA"
     gene            complement(5663..6331)
                     /gene="basR"
                     /locus_tag="OQQ66_002607"
     CDS             complement(5663..6331)
                     /gene="basR"
                     /locus_tag="OQQ66_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1905487.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6328..7971)
                     /gene="eptA"
                     /locus_tag="OQQ66_002608"
     CDS             complement(6328..7971)
                     /gene="eptA"
                     /locus_tag="OQQ66_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1905488.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8075..9412)
                     /gene="adiC"
                     /locus_tag="OQQ66_002609"
     CDS             complement(8075..9412)
                     /gene="adiC"
                     /locus_tag="OQQ66_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1905489.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(9549..10310)
                     /gene="adiY"
                     /locus_tag="OQQ66_002610"
     CDS             complement(9549..10310)
                     /gene="adiY"
                     /locus_tag="OQQ66_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1905490.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(10636..12903)
                     /gene="adiA"
                     /locus_tag="OQQ66_002611"
     CDS             complement(10636..12903)
                     /gene="adiA"
                     /locus_tag="OQQ66_002611"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1905491.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13102..14010)
                     /gene="melR"
                     /locus_tag="OQQ66_002612"
     CDS             complement(13102..14010)
                     /gene="melR"
                     /locus_tag="OQQ66_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1905492.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14293..15648
                     /gene="melA"
                     /locus_tag="OQQ66_002613"
     CDS             14293..15648
                     /gene="melA"
                     /locus_tag="OQQ66_002613"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1905493.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            15751..>15938
                     /locus_tag="OQQ66_002614"
     CDS             15751..>15938
                     /locus_tag="OQQ66_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1905494.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMG"
BASE COUNT         3815 a         3870 c         4219 g         4034 t
ORIGIN      
        1 gaaaatggcg tcgctttcga aaaatattat ctgggtgccc agcctatttt tcattgtctg
       61 aaaagtttga atgatttagt gcgactggcg aaggcgctgg acgtcgattt tcctttttct
      121 gcttacgccg ccattgagca tattcccgtg attgaagtgg agtttgtcca tctggcgggg
      181 ctggaaagtt atcccggtca gttgacgtta ctggataccc ccgggccaaa tgaagccggg
      241 caaccgcatc tgcaaaaaat gcttaaccag cagctggcac gcgcctcggc ggtactggcg
      301 gtgctggatt atacgcaact gaaatcgatc tccgatgaag aggtccgtga ggcgattttg
      361 gcggtggggc aatcggtgcc gctgtatgtg ctggtcaata agttcgatca acaggatcgt
      421 aacagtgacg acgccgacca ggtgcgggca ctgatttccg ggacgctgat gaaaggctgt
      481 attacgccac agcagatatt tccggtgtcg tcgatgtggg gctacctggc gaatcgggcg
      541 cgctatgagt tagccaacaa cggtaagtta ccagcgccag agcaacaacg ctgggtggaa
      601 gattttgccc atgccgcgct cggcaggcgc tggcgtcatg ccgatctggc ggacctcgaa
      661 catattcgtc atgctgccga tcagttgtgg gaagattcgc tgttcgccca gccaattcag
      721 gcgttgcttc atgccgctta cgctaacgcc tcattgtatg ctctgcgatc tgctgcgcat
      781 aaactgttga attacgcgca gcaggcgcgg gaatacctgg attttcgtgc gcacgggtta
      841 aacgtcgctt gtgaacaatt gcggcaaaat atccatcagg tcgaagaaag tttgcagcta
      901 ttgcaactca atcaggcgca ggtgagcggc gagattaaac atgaaatcga gctggccctg
      961 acctccgcca accactttct gcgtcaacag caagatgcgc tgaatgcgca gttagccgcc
     1021 ttgtttcagg atgattcgga gccgttaagc gagattcgta cctgctgtga gacactgtta
     1081 cagacggcgc agaacaccat cagtcgcgac tttacgctgc gttttgccga gcttgaatcc
     1141 accctttgcc gggtgttaac cgatgttatt cggcccattg agcaacaagt caaaatggaa
     1201 ttgagcgagt cagggtttcg tcctgggttt cattttcctg tttttcacgg agtagttccc
     1261 cacgtcaaca ctcgccagct gttcagtgaa gtcatttcgc gccaggaggc aacggacgag
     1321 cagagcacgc gtttaggcgt tgtgcgtgag actttttcgc gctggttgaa tcagcccgac
     1381 tggggacggg gaaatgagaa atccccgaca gaaacggttg attacagtgt gttgcaacga
     1441 gcattaagcg cagaagtcga tctttattgc caacaaatgg ctaaagttct ggcagagcag
     1501 gtcgatgaat ctgttacggc aggcatgaat acttttttcg ctgagttcgc ttcatgtttg
     1561 acggaattac agacgcgttt acgcgaaagc ctggctctgc gtcaacaaaa tgaatcggtg
     1621 gtcaggctga tgcagcagca attgcagcag actgtgatga ctcacggctg gatttacacc
     1681 gacgcccagc tgttacgcga tgatattcaa acacttttca cggcagaacg atattgacca
     1741 agacgttact ggacggcccc ggtcgcgtgc tggagtcggt ttatccccgc tttttagtgg
     1801 atctggcgca gggcgatgat gcccgccttc cacaagccca tcagcagcag tttcgtgaac
     1861 gactgatgca ggaacttctt tcgcgtgtgc agcttcagac atggacgaac ggcggcatgt
     1921 taaatgcgcc gcttagcctg cgactgacaa tggtggaaaa actggcgtcg atgctggatc
     1981 ccggtcatct ggcgctgacg cagatcgcgc agcatctggc gctgctgcaa aaaatggatc
     2041 accgccagca ctctgctttc ccggagctcc cccagcaaat tgccgccttg tatgagtggt
     2101 tttcagcccg ttgtcgctgg aaggaaaagg cgttaacgca acgaggccag gcctactggt
     2161 gcaggcaggt gatcagagcg agcaaatttt tacccgctgg cgtgctgggg cgtataacgc
     2221 ctggtcgttg cctgggcgct gttttatcgt tctcgaagag ttgcgttggg gggcatttgg
     2281 cgatgcctgt cgtctgggaa gcccgcaagc ggtggcgttg ctgctgggtg atttgcgcgt
     2341 gaaagcgaca caacatctgg cagagagtat caatgcggcc ccgaccacgc gtcactatta
     2401 ccatcagtgg tttgcctctt cgaccgttcc gacgggcggg gatcatgctg attttttaag
     2461 ttggctggga aagtggacca cggcagataa acaacccgtt tgctggtcag tgacccaacg
     2521 ttggcaaact gtcgcgctgg ggatgccacg actctgttca gcgcagcgtt tggtgggggc
     2581 aatggtcgag gaaatcttct ctgtaaattt ggcgtaaata atcagttaca tcaatgagtc
     2641 ctaaacgaaa tccatgcgtg aagttgatca caaatttaaa tactggtagg gtaaaaaggt
     2701 cattaactgc ccaattcagg cgtcaactgg tttgattgta cattccttaa ccggagggtg
     2761 taagcaaacc cgctacgctt gttacagaga ttgcatcctg caattcccgc tccccttttg
     2821 cggccgtcgc gctgattttt ctggcgtttg cggaaatggg ccaactctgc gaggaaagct
     2881 atgctgaaaa ggaaaaaagt aaaaccgatt acccttcgtg atgtcaccat tattaatgac
     2941 agtaaactgc gtaaagccat taccgcagca tcactgggta atgcaatgga atggttcgat
     3001 tttggtgttt atggttttgt tgcttacgca ttaggtaaag tttttttccc gggggctgac
     3061 cccagcgtgc agatggttgc tgcacttgcc actttctccg ttccctttct gattcgaccg
     3121 cttggcggac tcttctttgg tatgttgggc gataaatatg gtcgccagaa gatcctcgct
     3181 atcactattg tgattatgtc gatcagtacg ttctgtattg gcttaatacc gtcctacgac
     3241 acgattggta tttgggcacc gattctgctg ttgatctgta agatggcaca aggtttctcg
     3301 gtcggcggtg aatataccgg ggcgtcgata tttgttgcgg aatactcccc tgaccgtaaa
     3361 cgtggcttta tgggcagctg gctggacttc ggttctattg ccgggtttgt gctgggtgcg
     3421 ggcgtggtgg tgttaatttc gaccattgtc ggcgaagcga acttcctcga ctggggctgg
     3481 cgtattccgt tctttattgc tctgccgtta gggattatcg ggctttacct gcgccatgcg
     3541 ctggaagaaa ctccggcgtt ccagcagcat gttgataaac tggaacaggg cgaccgcgaa
     3601 ggtttgcagg atggcccgaa agtctcgttt aaagagattg ccactaaata ctggcgcagc
     3661 ctgttgacat gtattggtct ggtaattgcc accaacgtga cttactacat gttgctgacc
     3721 tatatgccga gttatttgtc gcataacctg cattactccg aagaccacgg ggtgctgatt
     3781 attatcgcca ttatgatcgg tatgctgttt gtccagccgg tgatgggctt gctgagtgac
     3841 cgttttggcc gtcgtccgtt tgtgctactt ggtagtgttg ccctgtttgt gttggcgatc
     3901 ccggcgttta ttctgattaa cagtaacgtc atcggcctga tttttgccgg gttactgatg
     3961 ctggcggtga tccttaactg ctttacgggc gttatggctt ctaccttgcc agcgatgttc
     4021 ccgacgcata tccgttacag cgcgctggcg gcggcattta atatttcggt gctggttgcc
     4081 ggtctgacgc caacactggc ggcctggctg gtcgaaagct cgcagaatct gatgatgcct
     4141 gcctattacc tgatggtagt ggcggtgatt ggtttaatca ccggcgtaac catgaaagag
     4201 acggcaaatc gtccgttgaa aggtgcgaca ccggcggcgt cagatataca ggaagcgaag
     4261 gaaattctcg tcgagcatta cgataatatc gagcagaaaa tcgataatat tgaccacgag
     4321 attgccgatt tgcaggcgaa acgtacccgc ctggtgcagc aacatccgcg aattgatgaa
     4381 taagctgaaa cggatggcct gatgtgacgc tgtcttatca ggccaattga actcttaagg
     4441 ttcacttaat ctctgacgcg catactctcc tccaggttaa cggaggagag tgcaatgaaa
     4501 aaccgtgttt atatgaaagt ttaactaccg tgttcagcgt gctggtggtc agcagctttc
     4561 tttatatctg gtttgccacg ttctgatctt tcttcagccg tacctaggcc cgcgtgccgg
     4621 aagtctcttg ccggttttgc aggaaaaact gcccgtgatg caactgtgta atgcggctga
     4681 caatacttaa ccccagacca atcccgccat aacggctgtc catacgtaca aacgctttac
     4741 tcaactcccc gcatttactc tcatcaatac ctggtccttc atcttcaact gccatgaccg
     4801 ctccgccatc ttcttgcagc ttaatcataa tgttgctgcc ttgcgggctg taacgatggg
     4861 cgttttctac caggtttcgc aataacatcc gcagcagggt tgcatcaccc tgaacggtga
     4921 tgtcggcggc gctctctggc aatagcaggg tttgctgtcg ctggtcgagc atggtactga
     4981 gttcgtcata cgaggggaga atgacatctt ccagcagttt tacatgttga taattaccgg
     5041 aagaaaatga ctgtccggca cgcgccagtt gcagcagctg ggagacgctc tccatcatct
     5101 gatcaagccg tgccactaac ggtgctacat caatgtgatg cgttttcgcc agcagttcca
     5161 gatgcaaacg cacccccgcc agtggcgttc gcagttcgtg cgcgacgtca gcggtaaaca
     5221 acctttcgtt atccagcgtg ctggtcaggc gactgaccag atcgttcaac gccgaaacca
     5281 ccgcttcgat ttcgagggtg gcgctgtgaa tggcaatggg cgttaagttg tcggcagtgc
     5341 gcgcttccag ctctttttgc agctccgcca gcgggcgggt gatgcggcgt accgcctgat
     5401 agcagataaa tagcgtcagg ctgaccataa agacgccggg gacaatcagg ctggcgaccg
     5461 cctcgcggat ctcacgcatg atgtggcgat cgttgttgcg attgtcgcgt agcgcctgct
     5521 caaacagctg aatctgctcg gtactttcat gccatagcca gaagacgctg atcagctcaa
     5581 acaccaacaa aatggccccg atagtcaata tcagccgttg gcgcagcgat attggtcggc
     5641 gcagaaaacg catcagattc aattagtttt cctcattcgc gaccagcata tagccaaagc
     5701 cgcgcacggt gcggatacgg gatttgccca ctttgtcgcg cagattgtgg atatgcactt
     5761 ccagggtgtt ggtcgagggt tcattgtccc agttatagat gtcgttgtag agaatttccc
     5821 gatgtaccgg actgcctgct ttgagcatta accgtgacag cagagcatat tctttgggcg
     5881 tcagaatcaa ctcttcaccg cccatccata cctgacggcg acccatattc agcgtcagat
     5941 tgccaacaat cagctcactt tcgccctgat tattatggcg tcgtagcagg gcgcggatac
     6001 gggcgtgtaa ctcttccagc gcaaaaggct tcaccagata gtcatcggca ccgacatcca
     6061 gcccggcgat tttgtcggtc agcgtatcgc gagcggtgag gatcagtacc ggcagggtat
     6121 attttttctg ccggatacgg gcgagaaaat gcagtccatc ttcgtcgggt aaccctaaat
     6181 ccagtaccac caggctgtaa tgaccggctt caaggctttg ttccgccatc cgcgcggttg
     6241 tcacgccatc gcacgcgtag ccttcggttt gcgccgccag aatcagtccc tgcaataaca
     6301 gcgtatcgtc ttcaacaatc agaattttca tttactcact ctcctgcaag tttgcagaat
     6361 atcatccgca gcctggtaat acttcgtctc aacgccagtt aatcccaata gcgtggagaa
     6421 taaattgtct tgtgaatagt gttgcgtttg cgcctgtttt tgcaggcagt tctggtcaac
     6481 ctgataccgt ttttgataat cctccgacag ccacagcagc atcggcacct gtttttggct
     6541 atccggggcg atggcataag gcagaccgtg cagatagatg ccattttcac ctaacgattc
     6601 accgtggtca gaaagataaa ccaggctggt ggtaaattta tcctgatgtt ctttcagcag
     6661 attaatcgct ttatcaacaa tatagtcgac gtaaaccagc gtgttgtcgt aagtgttcac
     6721 cagttgctct ttggtacagg tctggatctc attggtgtcg caggttgggg taaatttcct
     6781 gaactgcggc ggatagcggt tgtaataggt cggaccgtgg ctgccgatgg tgtgtaagac
     6841 aatcacgcca tcaccctgca ggttattgat gtactcttcc agcccgtgga acagcacttc
     6901 gtcatagcat tcgccgttga tgcactgacc aggcagattc agcgcggtga cgttctggtg
     6961 aggcacgcgg tcgcaggcac ctttacagcc gccatcgttg tcattccaca gcacgttgat
     7021 gcccgctcgc tgaatgatat ccagcacgcc ttcctggtgc tgtgccagct cttctttgta
     7081 atgctcacgc ggcatatccg agaacatgca cggtactgaa actgccgttg ccgtgccgca
     7141 agatacggta ttagggaaat agaccacgtt atctttcgcc agccgcgggt tagtttcacg
     7201 cgggtagccg ttgagggaga agttctccgc ccgcgaggtt tcgccgacaa tcaggatggt
     7261 caaattttta cgtttttcgt tctgcattaa cgggttgcgg tgggcgtctt caccaattcg
     7321 caccagcggt agatttgcca gtcgctgatg ggagtaccat gaccagctgg caacaatgct
     7381 gttagagggg cttaaggatt tcaccagctc tttgttattg cggaacaacg aggcgtagtc
     7441 tttataaaac agtgcggcga ccagcaaaat cagtagtaca gaaaccagaa tattggctcc
     7501 acggaaaaga acactgcgca gacgcgaggt ggcaggtttg atttttatcc agcaggcaat
     7561 cagcgcagca agcacgccgc tgaatcccag cgttaataac atttgcggtg tcatcagcgc
     7621 ataactttct gccggagtgg tatcaataat attggcaatc atcgagcggt cgatgacgat
     7681 gccgtaagtc attatgaaat attgtgcagc cgcgccaacc agaataaaca ggcaggccag
     7741 tggtcgatta agccataaga aagagcttag tgtcaggaca atattaatca cgctgaaagc
     7801 gacgaccggc atcgacaaga aaaccagtac gttatgcagc gaatccagcg gcagcgcctg
     7861 caacacctgt ttaaaaaagg caatattcag gcagatagag atataaaaag cggccaacaa
     7921 tagccaggcg agtaaattca aagagggtct ttttagtagg cgcttcaaca tcacggtgtt
     7981 tccatcgaac aaagtgcgca tatgctcgca aagcaaaatt aagccaacct taagttctta
     8041 aggttggctt ttatgtttgc tggattaagg ggaattaatc tttgcttatt ggtgcatcta
     8101 agggatacgg gtttttatgt agccggttgt aattcagcgc atacatggcg gtgatgacca
     8161 tcagggtgac aaatgaccac ataacctctt tcgctccgga ccccaccacg gcccagatgc
     8221 agtagaggaa ggcaatggta gtaactgcca gatatgccgg gcgtgcttta ccaaagtgac
     8281 cgtgtccgag cagcagtaac gccgcacagg tgtaaagata tggcaccagt gtaaagatga
     8341 ccgagacgga agaaaccaga ccgaactctt tggtcgcgtt tggtgaaatg ctgctgagct
     8401 ggaagatggt catcaaaata ccgacgataa tcagccccgc cactggcgta cccgctttat
     8461 ttacacgggc aaaaatcggt gggaacagtc cgtcatcggc agcggctttc gccgtttgac
     8521 ccgccagcaa cgtccagccg cccagtgaac ctaagcaacc cgcagctgcg cagaaggaaa
     8581 caatggcccc gggggtgtca cccaacgcca tccgtgcggc atcaccgaat ggcgaagcag
     8641 aaacgcgcag tgcggcatta gggatcatcc ccataatcgc ggtggtagaa agtacatagc
     8701 aaacggcggc aatcaatacc ccaccaatgg tggcgatagg gacattgcgt ttcgggtttt
     8761 tcaccacacc tgcggcaacg gaggcacttt ccacaccgat gaacgaccac agcgtaacgt
     8821 taagggtact ttgaattgca ccgaaggtac ccaggccgct gacgttccat gccgccatat
     8881 aggtttcacc acggaaccag aaccagccaa atacggcaat cccgacgatg gggatcagcg
     8941 ccagcacggt ggcaactgcc tgcacacggg tgatcatttt cggaccaaca atgttcagca
     9001 ggacgaagat ccacagcacc acgacgcagg tgatggttaa taccaatgga tctttcagaa
     9061 tcgggaagaa gtaacttaaa tatcctacgc caatgaccac catggcgata ttgccgatcc
     9121 agcaggccag ccagtagagg acgttggttt gataaccgag aaacgggcca aagcagcggc
     9181 gggcgtaagc gtaagaacca ccaggacttg ggtcgaggaa cgacattttg gcgtatacca
     9241 tcgagagccc cagtgcaccg ataatcgtca ccaaccatcc ataaatggca atcccgccag
     9301 tggaggccag gtttgcaggt aacagaaaaa cacctgaccc cataatattc cccgacacca
     9361 tcagggtgac ggggattaag cccactttgt gagcatcagc atccgaagac ataattaaac
     9421 tcctgcgaag gcgagcttcg tgacaataaa ttacgtcata tcatacgcct gcattcgata
     9481 gttgaattat tatcgaccgg gttatcgctg atgccgttat taatatagct cttcgttaag
     9541 gttaaaaatc aggcgacagt gcgttctctg tgctgactaa cataatgcag cggcgtcata
     9601 ccgtagaagt ctttaaagac agaaataaag tacgacgtac tgttgtagcc gcaggactgt
     9661 gatacctgag agatattttt accgtccatc attaattcat ttacggcata acgcatgcgg
     9721 caggtggtga ttatttggct ataactggtg ttttcgcttt tcagcttttt ctttaacaga
     9781 cttgggctaa gacataaaca actggctacc atactaagat tccagtcttt gtgaatatcg
     9841 ctttcaataa tttgataaac gctgtcactt acacaattac gtaacatata catcattaat
     9901 gaaaggaatt ttttactgtc gagaaaacgg gatagcaccg tgaataataa tgcgcgtgtt
     9961 cgttcctttt ctgactcagt ttctgcgggc atcatgctgt gttgcgcggc aacgcgaaag
    10021 acgtctggcg tcaggcatgg cagggtgagt atgggcgtag cgctacgttg ccatactggt
    10081 atttgcgaga gatctttatt cagaaaccgg aggtaatctt tgatgatgtc gtgactaata
    10141 tgagcaacca aagtgttgtt aagagaggag acgtcgataa tattattttc acaatttaat
    10201 aacgccatat ggttagcttt aaggctaata ggttctttcc cattcaccct tatccagaca
    10261 tctttttcag tcaataaaac aatacaaggt tggtcgctgc aaatcctcat acatgtactc
    10321 ctgagtgcga ataaaagacc agttctcgct aaagcaaagc gataccggga aatggttata
    10381 taattgtatg acgaatataa acatgcataa aaataacgag ttttactaat attacaaatt
    10441 tttatagtaa tttctcatgt acatattttt ttacagggta aataacaggc aaaaaaaaag
    10501 cgcggggtgg ggccgcgcaa aaagtatgac atttttgttt gcagcaattt ttatgaaaac
    10561 gttgccatac tcaaaaggcc taaggccagg cggcgtaatg ttatttaaac aattacgcct
    10621 tcagcggaat agtggttacg ctttcacaca catagcgtgg taaataccgt caataatttc
    10681 agtcccttca gtttcgtgtt caaatccagg gaaatggtgg tcccaggatt gcagcgagcg
    10741 taaataactt acttgcggac tgtttttatc gccgaagttt tcaccagaca gcagcatcgg
    10801 gattcctggc ggatatggga taactgagtt tgccgcgatg cgtcctggca gattttcaat
    10861 ggataccagt tcgacattgt tgtcgacaat cgcgttgtac gcttcacgcg gggtgatttc
    10921 cgccaccggc aggccggaat aggcttcgtt caaccgtgcg ccagggttgt tttctttcag
    10981 ccaggcaaac atggtgtcac ccagatcgtg aatccccatg ttcgcgtaag tgtcaggata
    11041 ttgttcaaca agttccggca tcacctgcgc cagcggtgtg ttggcgtcat agtggcgttt
    11101 gaaggagcaa agggtgttaa ccagagtccc ccatttccca cgggttacgc ccatagagaa
    11161 caggaacata atttggaagt cagtggtgcg ggtaggcaca atgccgtggc gaccaagcca
    11221 ggcagtgacc agcgccgccg gaacaccggt ttcttccagt tcaccatctt cacccattcc
    11281 cggagcaagg atgctgactt taatcgggtc gagcatactc cagttatccg gaatatcttt
    11341 gaagccgtgc cagctttcgc ccggatgcat tacccagtag tcctgaacgg tggtcagcag
    11401 tttggttggt gcgtcagcaa agtcataggt tttgccggtt tgtgggtcgg tgacgacttc
    11461 tttgttccac ggtttgaaga accagctacc gtcagcggtg aactctttat acagccgcgc
    11521 catcgcctga cggaaatcaa ccgcttcgtc gatcacttcc tgcgtcagtg acaggccgct
    11581 gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg cagatggcat acagcgggga
    11641 ggtggtggca tgcatcatgt aggcctggtt gaagcgggag aagttaatcg ccccacgacc
    11701 ttcacgtaca tgaatataag aagcctgtga aagcgcattc agcagtttgt gggtggagtg
    11761 ggtggcgaaa acggtaggac cgttgtgatc gccaggttcg ccgcgcatgg catagtgatc
    11821 ggcatagatc gggttgaaac gtgcatagcc gtaccaggct tcgtcaaagt gcagacgatc
    11881 ggaggttttt tccagcagat cctgcgcttc tttagcgtta taacacacgc cgtcataggt
    11941 gcagttggtc accacgcagt aagacggttt ttgcccggct ttgtctttag tcagcgggct
    12001 ttcactgatt ttcttctgca aggtttcagg ttgcatttcc tgcggataga ttggcccgat
    12061 aatgccgtag cggttgcggc ttggcaccat gtagaccggt ttcgcgcctg tcagcatcaa
    12121 gccttgttcg atggatttat ggcagttacg gtcaacgacc acgacatcgt tatcggtcat
    12181 gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg acgactaccg accaggagcg
    12241 atcggcacca aatacgcgtg cggcatattt ttcgctttcg ccaaatgcgc cagtatggtc
    12301 aagcaaagaa ccgagggaag ttcgttcgat gcccatatcg gtgcggaaca gattttctcc
    12361 atagtagtca tggtagaaac gtccggcggg tgttttggta aaaccaacgc cgccctggtg
    12421 gcctggcgct gcccaggaat attcatggat gtcgctatac ttcatcagcg cgctgaacag
    12481 tggcggcaac agctgctggc ggtagcgggt catcgcggca acggcgcgtc cggcgataaa
    12541 gtcggtggta tcttccagaa tccaggcgaa ttcatcgaca agctccagca ggtcgcgatc
    12601 cattgcggcg agggcttttt cccgatcgcc caacaggaag accggcacgt tttgttggcg
    12661 ctcatgaagc ttaccgatca attgtctgac gttttgatgt tcgtccggat gttccatttg
    12721 atagctgaac atcaggcagt caatggcttc gtttgaagag agaatggcaa aaccatcatc
    12781 aaaggaggtg gatttaatca cggtaacatt ttgctggctt aaagcatctg ccagacgctc
    12841 aacggcgtta ccgacccagg tgtcttgatg gagaaactcg ctttcaacaa ttaatacttt
    12901 catcattgct tacccggtta tgaaggaatc ttcgttgcgg gcaagtatct tccggccttt
    12961 tcgggctgta aagcgcgtga aaaacaaaca tttttgaaat atttattttt ataaacaatg
    13021 cgttatgtga ttattttaaa ttattttcac tgcttgttat cgacggctaa actatttttt
    13081 tggctgatac tgatatcgtc tttagccggg aaacgtctgg cggcgctgtt ggctaagttt
    13141 gcggtattgt tgcggcgaca tgccgacata tttgccgaac gtgctgtaaa aacgactact
    13201 tgaacgaaag cctgccgtca gggcaatatc gagaatactt ttatcggtat cgctcagtaa
    13261 cgcgcgaacg tggttgatgc gcatcgcggt aatgtactgt ttcatcgtca attgcatgac
    13321 ccgctggaat atccccattg catagttggc gttaagtttg acgtgctcag ccacatcgtt
    13381 gatggtcagc gcctgatcat agttttcggc aataaagccc agcatctggc taacataaaa
    13441 ttgcgcatgg cgcgagacgc tgtttttgtg tgtgcgcgag gttttattga ccagaatcgg
    13501 ttcccagcca gagaggctaa atcgcttgag catcaggcca atttcatcaa tggcgagctg
    13561 gcgaatttgc tcgttcggac tgtttaattc ctgctgccag cggcgcactt caaacgggct
    13621 aagttgctgt gtggccagtg atttgatcat catgccgtga gtgacgtggt taatcaggtc
    13681 tttatccagc ggccaggaga gaaacagatg catcggcaga ttaaaaatcg ccatgctctg
    13741 acaggttccg gtatctgtta gttggtgcgg tgtacaggcc cagaacagcg tgatatgacc
    13801 ctgattgata ttcacttttt cattgttaat caggtattcc acatcgccat cgaaaggcac
    13861 attcacttcg acctgaccat gccagtggct ggtgggcatg atatgcggtg cgcgaaactc
    13921 aatctccatc cgctggtatt ccgaatacag cgacagcggg ctgcgggtct gtttttcgtc
    13981 gctgctgcac ataaacgtat ctgtattcat ggatggctct ctttcctgga atatcagaat
    14041 tatggcagga gtgagggagg atgactgcga gtgggagcac ggttttcacc ctcttcccag
    14101 agaggcgagg ggagcgtccg agcatcatga ggccgaaaac tctgcttttc aggtaactta
    14161 ttaccataaa ctcagattta ctgctgcttc acgcaggatc tgagtttatg ggaatgctca
    14221 acctggaagc cggaggtttt ctgcagattc gcctgccatg atgaagttat tcaagcaagc
    14281 caggagatct gcatgatgac tgcacccaaa attacattta tcggcgctgg ttcgacgatt
    14341 ttcgttaaaa atattcttgg tgatgtgttc catcgagagg cgctgaaaac ggcgcatatt
    14401 gccctgatgg acattgatcc cacccgcctg gaagagtcgc atattgtggt gcgtaagctg
    14461 atggattcag caggggccag cggcaaaatc acctgccaca cccaacagaa agaagcctta
    14521 caggatgccg attttgtcgt ggtggcattt cagattggcg gttatgaacc ttgcacggtg
    14581 actgatttcg aggtctgtaa gcggcatggt ctggaacaaa ccattgccga tacgttgggg
    14641 ccgggcggta ttatgcgcgc gctacgtacc attccgcatc tgtggcaaat ttgcgaggac
    14701 atgacggaag tctgccccga tgccacattg ctcaactacg ttaacccaat ggcgatgaat
    14761 acctgggcga tgtatgcccg ctatccgcat atcaaacagg tagggctgtg ccattcggtg
    14821 cagggaacgg cggaagagct ggcgcgtgac ctcaatatcg atccgtccac gctgcgttac
    14881 cgttgcgctg gtatcaacca tatggcgttt tacctggaac tggagcgtaa aaccgccgac
    14941 ggcagttacg tgaatctcta cccggaactg ctggcggctt atgaagcagg gcaggcaccg
    15001 aagccacata ttcatggtaa tactcgctgc cagaatattg tgcgctacga aatgttcaaa
    15061 aagctgggct acttcgtcac ggaatcgtca gaacattttg ccgaatatac gccgtggttt
    15121 attaagccag gtcgtgagga tttgattgag cgttataaag taccgctgga tgagtacccg
    15181 aaacgctgcg tcgagcagct ggcgaactgg cataaagagc tggaggagta taaaaacgcc
    15241 tcccggattg atattaaacc gtcacgggaa tatgccagca caatcatgaa cgctatctgg
    15301 actggcgagt cgagtgtgat ttacggcaac gtccgtaacg atggtttgat tgataacctg
    15361 ccacaaggat gttgcgtgga agtagcctgt ctggttgatg ctaatggcat tcagccgacc
    15421 aaagtcggta cgctaccttc gcatctggcc gccctgatgc aaaccaacat caacgtacag
    15481 acgctgctga ccgaagctat tcttacggaa aatcgcgacc gtgtttacca cgccgcgatg
    15541 atggacccgc atactgccgc cgtgctgggc attgacgaaa tatatgctct tgttgacgac
    15601 ctgattgccg cccacggcga ctggctgcca ggctggttgc accgttaaaa cgcgactaaa
    15661 cgctactgcg ccgggggatt tattccggcg cacacctctg acgataccaa taacagaagg
    15721 cgggcgttgg taacagcgac ccgataccct atgagcattt caatgactac aaaactcagt
    15781 tatggatttg gagcgttcgg gaaggatttt gcgatcggca ttgtgtatat gtacctcatg
    15841 tattactaca ccgatgtcgt tgggctgtct gtgggtttgg tcggtacttt gtttctggtg
    15901 gcgaggatct gggatgctat taacgatccg attatggg
//
LOCUS       DAJVTY010000095        15636 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVTY010000095 DAJVTY010000000
VERSION     DAJVTY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15636
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.095"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            333..425
                     /locus_tag="OQQ66_002615"
                     /pseudo
     CDS             333..425
                     /locus_tag="OQQ66_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            429..608
                     /locus_tag="OQQ66_002616"
                     /pseudo
     CDS             429..608
                     /locus_tag="OQQ66_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            942..3221
                     /gene="ydeP"
                     /locus_tag="OQQ66_002617"
     CDS             942..3221
                     /gene="ydeP"
                     /locus_tag="OQQ66_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1905495.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3469..3666
                     /gene="safA"
                     /locus_tag="OQQ66_002618"
     CDS             3469..3666
                     /gene="safA"
                     /locus_tag="OQQ66_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1905496.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3741..4502
                     /gene="ydeO"
                     /locus_tag="OQQ66_002619"
     CDS             3741..4502
                     /gene="ydeO"
                     /locus_tag="OQQ66_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1905497.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4904..6586
                     /locus_tag="OQQ66_002620"
     CDS             4904..6586
                     /locus_tag="OQQ66_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1905498.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6638..7795
                     /locus_tag="OQQ66_002621"
     CDS             6638..7795
                     /locus_tag="OQQ66_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1905499.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISNDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7761..7883)
                     /locus_tag="OQQ66_002622"
     CDS             complement(7761..7883)
                     /locus_tag="OQQ66_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905500.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8098..9783
                     /locus_tag="OQQ66_002623"
     CDS             8098..9783
                     /locus_tag="OQQ66_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCS1905501.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     LLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9821..12193
                     /locus_tag="OQQ66_002624"
     CDS             9821..12193
                     /locus_tag="OQQ66_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1905502.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12250..15033
                     /locus_tag="OQQ66_002625"
     CDS             12250..15033
                     /locus_tag="OQQ66_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1905503.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15395..>15636
                     /locus_tag="OQQ66_002626"
     CDS             15395..>15636
                     /locus_tag="OQQ66_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1905504.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4555 a         3400 c         3424 g         4257 t
ORIGIN      
        1 cgtggtgacg gcagttgaaa ccaatgtgtt gaggttgcat ggacggttaa gccgagaata
       61 cggtagtaag tgagatatgg ggtttggtcg ttgttaatga gcaaccatga ccgccaacga
      121 ctggattctc ccttggttta tgttggcgcg cataatgtat attatgttaa atcatatctg
      181 agtgatgcga agtgcttttc tggtagtcgt tattcgttca gggttccaga acttaagcct
      241 ctcctctttc ataattggcc tgtgcatgac ggatataggc tggctggtat gcagatttgc
      301 cgtttgccgc atcttcccag cgttgaagac tcaagctggc aaaccgcgtg caaatgagca
      361 cgccctcttt ccatcaacac ttcaggcagc ttgcgggaat gagtccacta cggtatcaga
      421 aatgggaagt gggtgtctct ctgatgcgta atggaaaaat actggctacg ggagaaaatg
      481 tctccctggg gacagtaaat aaaagcaaag tatctctggg tttaagcgca acctatggac
      541 aaacaggaaa caaggttgca gccggaaccg tccagtcagt cattggtgtc acttttatct
      601 atgaataatt gtgattttga tcttagtcta tgcgctccat tcacgaaaat tgcgcggata
      661 attattttgt gaaggctatt atcctacacc tgtaagaaaa tccgcgcaca attgaagcca
      721 gagtcttatt ttttatattg aaaaatatgt taatttaaac actatgagag atgatgctta
      781 acttatcgta tttaatctat tgtttaacga taatagatta aaaatagaaa gagagtagtc
      841 ccctactctt atatatccat gttggcgata atccctttgt atgtactgtg catcatcgct
      901 attacaaatc ctaataattc atttccacac aggataagta gatgaagaaa aaaattgaat
      961 cctaccaggg tgctgcaggt ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta
     1021 agcagatgga tatacgccag gatgttattg ccatgtttga catgaataag ccagagggct
     1081 ttgactgtcc gggttgtgca tggccagatc ctaagcacac tgcgtcattc gacatttgtg
     1141 aaaacggcgc aaaagcaatc gcctgggaag tcacggataa gcaggtaaat gcctctttct
     1201 ttgctgagaa tacggttcaa tcattactta cctggggaga tcacgagctt gaggctgcgg
     1261 gacgtctcac tcagcctttg aaatatgatg ccgtcagcga ctgttacaag ccattaagct
     1321 ggcaacaagc tttcgacgaa attggcgcac gccttcaaag ctatagtgat cccaatcagg
     1381 ttgaattcta tacttcgggc cgcacttcca atgaagctgc ctttctttat cagctttttg
     1441 cccgtgaata cgggagcaat aactttcccg actgctccaa catgtgccat gaaccgacca
     1501 gtgtgggttt ggcagcgagt atcggcgtgg gtaaagggac tgtgttgctg gaagactttg
     1561 agaagtgcga tttagtcatt tgcattgggc ataaccctgg tacaaaccac ccccgcatgc
     1621 tgacttcgtt gcgcgcttta gtgaaacggg gagcgaaaat gatcgccatc aatcctttac
     1681 aggaacgtgg cctggagcga tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact
     1741 ctgagactca gttggccagt gcgtactaca acgtgcgcat tggtggcgat atggcgttgc
     1801 tcaaggggat gatgcgcctg ttaattgagc gcgatgatgc tgcaagtgcc gcaggtcggc
     1861 cctcattgct tgatgacgaa tttattcaaa cgcataccgt cggctttgac gagctacgcc
     1921 gtgacgttct caattccgag tggaaagata tcgaacatat ttctggacta agtcagacac
     1981 aaatcgccga actggctgac gcatatgccg ctgccgatcg caccattatc tgttacggaa
     2041 tggggatcac tcagcacgaa catggtaccc agaatgtaca gcaactggtc aatctgctgt
     2101 tgatgaaagg taacattggc aagcctggtg cgggtatctg cccactacga ggtcactcta
     2161 atgtacaggg cgaccgaacc gtcggtatca ccgagaaacc gtctgcagag tttctggctc
     2221 gcctgggtga gcgctatggc ttcaccccac ctcatgcacc tggacatgct gcaattgcca
     2281 gcatgcaagc aatatgcacg gggcaggctc gagcattgat ctgcatgggg ggcaactttg
     2341 cactggcaat gccagatcgg gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg
     2401 tacacgtagc cactaagctt aaccgctctc atctgctgac cgcacggcat agctatattc
     2461 tgccggttct ggggcgtagc gagattgaca tgcaaaaaag cggtgcgcag gcggtaaccg
     2521 ttgaggattc aatgtcgatg attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa
     2581 tgctgaaatc agagtgtgca gtggtcgcgg gaatcgcgca ggcagcacta ccccagagcg
     2641 tggtagcctg ggagtatctg gtggaagatt atgatcgcat tcgcaatgac attgaagctg
     2701 tgttaccaga gttcgccgac tataaccagc gcatccgaca tcctggaggt tttcacctga
     2761 taaatgcagc tgctgaaagg cgctggatga cgccgtcagg taaggctaat ttcattacca
     2821 gcaaagggct gttagaagat ccctcttcag cgtttaacag taagctggtc atggcgacag
     2881 tacgcagcca cgatcagtac aacacgacga tttatggtat ggatgatcgc tatcgagggg
     2941 tattcggtca acgagatgtg gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa
     3001 aaaacggcga aagagttaat cttattgcgc ttacgccaga cggtaagcgc agctcacgcc
     3061 gcatggatag attaaaagtg gtcatttacc ctatggctga ccgctcactg gtgacctatt
     3121 ttccagaatc gaatcacatg ctaactcttg ataaccacga tccattaagt ggcattcctg
     3181 gttataaaag tattccggtt gaattagaac catcaaatta attcgcctcc tcatttcttc
     3241 tgctgtcatc cgcacagcag aagaatttct cattgactat tatcgcgcaa tttgctcaca
     3301 ttgatcaaat taaaccacac actataagat ataaacctct gcctacagct gtaagaaact
     3361 ccgctcagta ctgaagtgcc agtcctattt cctcttttct ccagcctgtt atattaagca
     3421 tactgattaa caatttttaa cgttatccgc taaataaaca tatttgaaat gcatgcgacc
     3481 acagtgaaaa acaaaatcac gcaaagagac aactataaag aaatcatgtc tgtaattgtg
     3541 gttgtcttat tactgaccct tacgttgata gccatttttt cggcaattga tcagctgggt
     3601 atttcagaaa tgggtcgcat tgcaagagat cttacacatt tcattatcaa tagtttgcaa
     3661 gactgaaaat aaacagcaaa ttataaatat gaaatgttaa aaaagtatcg ataaaaactt
     3721 tattgtttta aggagataaa atgtcgctcg tttgttctgt tatatttatt catcatgcct
     3781 tcaacgctaa cattttagat aaagattacg ccttctctga cggcgagatc ctgatggtag
     3841 ataacgctgt tcgtacgcat tttgaacctt atgagcggca ttttaaagag atcggattta
     3901 ctgaaaatac cattaaaaaa tatctacaat gcactaacat ccagacagtg acggtgcctg
     3961 ttcctgcgaa gtttttacgt gcttcaaatg taccgactgg attgcttaat gaaatgattg
     4021 cttatctcaa ctcggaagaa cgcaatcatc ataatttttc agaacttttg cttttttctt
     4081 gcctgtctat ttttgccgca tgcaaaggtt tcattacact attaactaac ggtgtgctat
     4141 ccgtttccgg aaaagtgaga aatattgtca acatgaagct ggcgcaccca tggaagctga
     4201 aagatatttg tgactgcctg tacatcagtg aaagcctgtt gaagaaaaaa ctaaagcaag
     4261 agcaaacgac attctcacag attctattag atgccagaat gcagcacgca aaaaatttga
     4321 tacgcgtaga aggttcagtc aataaaattg ccgaacaatg tggttatgcc agtacttctt
     4381 attttattta tgcgttccgc aaacatttcg gcaacagtcc gaagagagtt tctaaggagt
     4441 accgttgtca aagtcacacg ggtatgaata cgggcaacac gatgagtgct ttagctattt
     4501 gattatttgc taacgagtag tcaaccacgc acgctacgta agaattatat ggggcagcca
     4561 ttccctgccc cgcgttgttt ttaggcgata tatttattga aataaataag tgacatccat
     4621 cacatattta tgcacttgca taacctgttg catgattatt tatgttctca attcagcatt
     4681 ttgtcagtaa aatgcaataa tttattaaat atcaataaat tagttgttta tcggcgagaa
     4741 attacttaat agaacagaaa gtaatgtcaa cgctttatgg actgttattt cccttttttt
     4801 agctaaatct gctatctctt tatgtgacta acttcactta catccactta tttctcttcg
     4861 taaaattact tcggaattaa gtacaataag aagaggaaca tttatgaagt ctgcattaaa
     4921 gaaaagtgtc gtaagtacct cgatatcttt gatactggca tctggtatgg ctgcatttgc
     4981 tgctcatgcg gcagatgatg taaagctgaa agcaaccaaa acaaacgttg ctttctcaga
     5041 ctttacgccg actgaataca gtaccaaagg aaagccaaat attatcgtac taaccatgga
     5101 tgatcttggt tatggacaac ttccttttga taagggatct tttgacccaa aaacaatgga
     5161 aaatcgtgaa gttgtcgata cctacaaaat agggatagat aaagccattg aagctgcaca
     5221 aaaatcaacg ccgacgctcc tttcattaat ggatgaaggc gtacgtttta ctaacggcta
     5281 tgtggcacac ggtgtttccg gcccctcccg tgccgcaata atgaccggtc gagctcccgc
     5341 ccgctttggt gtctattcca ataccgatgc tcaggatggt attccgctaa cagaaacttt
     5401 cttgcctgaa ttattccaga atcatggtta ttacactgca gcagtaggta aatggcactt
     5461 gtcaaaaatc agtaatgtgc cggtaccgga agataaacaa acgcgtgact atcatgacaa
     5521 cttcaccaca ttttctgcgg aagaatggca acctcaaaac cgtggctttg attactttat
     5581 gggattccac gctgcaggaa cggcatatta caactcccct tcactgttca aaaatcgtga
     5641 acgtgtcccc gcaaaaggtt atatcagcga tcagttaacc gatgaggcaa ttggcgttgt
     5701 tgatcgtgcc aaaacacttg accagccttt tatgctttac ctggcttata atgctccgca
     5761 cctgccaaat gataatcctg caccggatca atatcagaag caatttaata ccggtagtca
     5821 aacagcagat aactactacg cttccgttta ttctgttgat cagggtgtaa aacgcattct
     5881 cgaacaactg aagaaaaacg gacagtatga taatactatt attctcttta cctccgataa
     5941 tggtgcggtt atcgatggtc ctctgccgct gaacggggcg caaaaaggct ataagagtca
     6001 gacctatcct ggcggtactc acaccccaat gtttatgtgg tggaaaggaa aacttcaacc
     6061 cggtaattat gacaagctga tttccgcaat ggatttctac ccgacagctc ttgatgcagc
     6121 cgatatcagc attccaaaag accttaagct ggatggcgtt tccttgctgc cctggttgca
     6181 agataagaaa caaggcgagc cacataaaaa tctgacctgg ataacctctt attctcactg
     6241 gtttgacgag gaaaatattc cattctggga taattaccac aaatttgttc gccatcagtc
     6301 agacgattac ccgcataacc ccaacactga ggacttaagc caattctctt atacggtgag
     6361 aaataacgat tattcgcttg tctatacagt agaaaacaat cagttaggtc tctacaatct
     6421 gacggatcta cagcaaaaag ataaccttgc cgccgccaat ccgcaggtcg ttaaagagat
     6481 gcaaggcgtg gtaagagagt ttatcgacag cagccagcca ccgcttagcg aggtaaatca
     6541 ggagaagttt aacaatatca agaaagcact aagcgaagcg aaataactaa accttcatgc
     6601 ggcggaattt tccgccgcct tattgagcga gatagcgatg cacgttacag ccaagccctc
     6661 cagttttcaa tgtaatctca aatgtgatta ctgtttttac cttgaaaaag agtcgcagtt
     6721 tactcatgaa aaatggatgg atgacagcac tctgaaagag ttcatcaaac aatatatcgc
     6781 agcgtctggc aatcaggtct attttacctg gcaaggcggt gaacccactc tggctggcct
     6841 ggattttttc cgtaaagtta ttcactatca acaacgctat gcaggccaaa aacgtatttt
     6901 taatgcatta caaacgaatg gcattttatt gaataatgaa tggtgttcct ttctcaaaga
     6961 acatgaattt ctggttggta tctcgaacga tggcccccag gagttacatg accgttacag
     7021 acgcagtaat tcaggtaacg gtacttttgc aaaagtgata gcagccatcg agcgtctgaa
     7081 atcatatcaa gttgagttta atacgttaac cgtcattaat aacgttaatg tccattaccc
     7141 tcttgaggtt tatcattttt taaaatctat cggcagtaaa catatgcaat ttatcgaatt
     7201 gctcgaaacc gggacgccga atattgattt cagtggtcat agtgagaaca cattccgtat
     7261 cattgatttt tctgtgcctc ccacggctta tggcaagttt atgtcaacca tttttatgca
     7321 atgggttaaa aacgatgtgg gtgaaatttt cattcgtcag tttgaaagct ttgtcagccg
     7381 ttttttgggg aatgggcata caagttgtat tttccaggag tcctgcaagg ataatctggt
     7441 tgttgaaagt aatggagaca tttacgaatg cgaccatttt gtctatccac agtacaaaat
     7501 tggaaacatt aataaatctg aactcaaaac gatgaacagt gtacaactga cagcgcaaaa
     7561 aaaacggatt tcagcgaaat gtcagcaatg tgcatataaa cctatctgca atggcggttg
     7621 tcctaagcat cgtattacta aagtaaacaa tgagactgtt tcttattttt gcgaaggtta
     7681 taaaatcctt ttttcaacca tggtacctta tatgaacgcc atggttgagt tagctaagaa
     7741 cagagtaccg ctttaccaca ttatggatgt tgcaaaacaa atggagaata attaataacg
     7801 ctctctttta actccattct tagcctcctt cttatgacct taacaaggca cccgacataa
     7861 attcctgtcg ggtgtttcgt catcatcatc ttcccttgcg aaaatcctgg cttacgtggt
     7921 atgcgtcagg gcattagcaa tacccttgat tatcaatagc ataaccataa ccataaccat
     7981 aaccactgaa aattaaaaat ttctatatat ttcatattgt taaatgatac ttaaataaat
     8041 ttcatcagta aatcattgcc atagataatc attggatggc acaatcaatt ttcgctaatg
     8101 ataaccattc ccattacgtt ttgtatgttg atagcaaagt atctctgtct gctgaagccc
     8161 ttttggctgc gtaagaataa taaaacttca gtgttgttaa tcattattat cctcgcaatg
     8221 atccttggcg tggttaaaat tcaggtctgg ttaaacgact ggaataatga ttttttcaat
     8281 gcgctaagcc agaaagaaac cgataagctt tggcagcttg ttctttggtt ccctgccctg
     8341 ctggggatct ttgtattaat atccgtaaac aaaacatggt taattaaact actgactatt
     8401 cgctggcgcg aatggttaac agattactac cttaatcgct ggtttgcaga taaaaactac
     8461 tacttcacgc agatctacgg cgagcataaa aatacagata accctgacca acgtatcgct
     8521 gaagacattc tcttactgat cagcaaaacg ctcagtcttt catttggctt catccagtca
     8581 cttagcatgc tgatcacctt taccgttatt ctttggcaaa gcgcgggtac gctctcattc
     8641 actgttggtg gaacagaatg gaatatccag gggtatatgg tctataccgt cgtgctcatt
     8701 gtgatcggtg gaactttatt tacccataag gttggtaagc gtattcgtcc gcttaatgtg
     8761 gaaaaacaac gtagcgaagc gacatttcga actaatcttg tgcagcataa taagcaggca
     8821 gaactgattg cgctaagcaa tgctgagtct ctacaacgcc aggagttgcg cgacaatttt
     8881 cataccatta aagagaactg gcatcgttta atgaatcgtc agcggttgct tgattactgg
     8941 caaaatattt attcgcgctc gcttagcgtt cttccctact ttctgttatt gccgcagttt
     9001 attagcgggc agatcaatct gggcggactg atgaaatcgc gccaggcatt tatgctagta
     9061 tcgaacaatt taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt
     9121 atcgatcgct tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa
     9181 aattgccaac atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc
     9241 atattagaga acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac
     9301 tctggtgcgg gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa
     9361 ggtgatattt tttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaacc
     9421 ggcttactga aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg
     9481 agcgaagtac tgcatcaggt tggtcttggg aaattggctg cgcgtattca tgaccacgat
     9541 cgctggggag atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt
     9601 ttacgacgtc cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag
     9661 gctatccgct tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt
     9721 acacatcaac ccggtgtctg gaacctggcc gatgatattt gtgacattag cgcggtttta
     9781 taaaaacaat aaaagccctg ctgtacggag atataaataa atgaagcgag ttcttattcc
     9841 tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc gatgacaaaa acatgtacat
     9901 gcattttttt gaagagatga cggtctatgc tcctgtccct gtacccgtaa acggcaacac
     9961 gcattacacc agtgaaagca tcgagcgttt accgaccggg aatggcaata tcagcgatct
    10021 gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa agtacctcgt tgaaccaggg
    10081 agatattcgc cctgagaaaa tctctattca cggtgcgtcg ccctaccaga atgcctattt
    10141 gattgacggt attagtgcaa ctaataacct gaacccagcg aatgagtccg atgccagtag
    10201 tgcaaccaat attagcggga tgtcacaggg gtattatctt gatgtcagct tactggacaa
    10261 tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt cgcttcaatg gcggggtaat
    10321 tgatgcaaag atcaaacgct tcaacgctga tgatagcaag gtgaaattgg gttatcgcac
    10381 tacgcgttcg gactggttaa catcgcatat cgatgagaat aacaagagcg catttaatca
    10441 aggttcttca ggaagtactt atttctctcc agattttaaa aagaactttt ataccttgtc
    10501 gtttaatcag gagctggctg ataactttgg cgttaccgcc ggtttatcgc gccgccagtc
    10561 tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt gtcgtcggtc gggcacagta
    10621 taaaaacgtt atcgatactg cattgagcaa atttacctgg tttgccagcg accgctttac
    10681 ccacgattta accttaaaat ataccggctc cagccgtgat tataatacca gcaccttccc
    10741 gcagtctgat cgcgaaatgg gtaataaatc ctatggtctt gcatgggata tggatacgca
    10801 actcgcatgg gccaaactac gtaccaccgt tggttgggat catattagtg attatacccg
    10861 tcacgatcat gacatctggt acaccgaact ttcatgtaca tatggtgata ttacagggcg
    10921 ttgtacccgt ggcggattag gacacatttc ccaggctgta gataattaca ccttcaaaac
    10981 acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg catcaaccct actccggcgc
    11041 ggaatacatc tattccgatg catggactga acgccataac cagtctgaat cctatgtgat
    11101 taatgctgca ggaaagaaaa ctaaccatac catttaccat aaaggtaaag gcagcctagg
    11161 aattgacaac tacacgctgt atatggcgga tcacattagc tggcggaatg tgtcgttaat
    11221 gcccggtgtg cgttatgact atgacaacta tctgtcaaac cacaatatct ccccgcgctt
    11281 tatgacggaa tgggatattt ttgctgatca aacctcaatg attaccgccg gttataaccg
    11341 ttactatggc gggaatattc ttgatatggg attacgtgat atccgcaata gctggacgga
    11401 atcggtatca ggtaataaaa ccctgacgcg ttatcagaat ttgaaaacgc cttataacga
    11461 tgaactggca atgggattgc agcaaaaaat cggtaagaac gttattgcac gcgcaaacta
    11521 tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa
    11581 aaccaccatt actgaatata acaacgatgg caaaaccaaa acgcattcgt ttaacctcag
    11641 ttttgaactg gccgaacccc tgcatatccg ccaggtagat attaacccgc aaattgtctt
    11701 tagctatatc aagagcaagg gcaacttgtc gttaaacaat ggttatgagg agagcaatac
    11761 cggtgataac caggtggttt ataacggtaa tctggtctct tacgatagcg ttccagtggc
    11821 agattttaat aacccattaa agatctcctt aaacatggat ttcacgcatc aaccgagcgg
    11881 gttagtgtgg gcgaatacgc tggcgtggca agaagcgcgt aaagctcgca ttatccttgg
    11941 taagacaaat gcgcaataca tcagcgaata ttcagattac aagcagtatg ttgacgaaaa
    12001 actggatagc agcctgacct gggacacccg cttgtcctgg acgccacaat ttctgaaaca
    12061 acaaaacctg acgatcagtg ccgatattct caatgtactg gatagcaaaa ccgctgttga
    12121 tacaacgaat accggtgtgg cgacctacgc cagtggccgt actttctggc ttgatgtcag
    12181 catgaaattt taatagttcg gattttactt aaccgggtaa attcgcccgg ttttcgcatg
    12241 gagataatta tgagaaacct ctgtttctta ctgacgttag tggcaactct gttgctcccc
    12301 gggcggctga ttgccgccgc cttaccgcag gatgaaaagt taattaccgg gcaactggac
    12361 aatggcttgc gatatatgat ttatccgcat gctcaaccaa aggatcaggt aaatttatgg
    12421 ctgcaaattc ataccggttc attgcaggaa gaagacaatg agcgcggcgt ggctcatttt
    12481 gtagaacata tgatgtttaa cggcacaaaa acatggccgg gtaataaagt catcgaaaca
    12541 tttgagtcaa tgggcctgcg ttttggtcgc gatgttaatg cctataccag ctatgacgaa
    12601 acggtgtatc aggtgagttt gccgactacg cagaaacaaa atctgcaaca agtgatggca
    12661 atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac tcgaagtaga cgctgaacgt
    12721 ggcgtaatta ctgaggaatg gcgtgcccat caggatgcga aatggcgcac ctctcaggcg
    12781 cgccgccctt tcctgctggc taatacccgt aatttagacc gtgaacctat cggcctgatg
    12841 gatactgtcg ccacggtcac accggcacaa ttgcgccaat tttatcaacg ctggtatcaa
    12901 ccaaataata tgacctttat cgtggtcggc gatatcgaca gtaaaaaagc gctggcgctg
    12961 ataaaggata atttaagtaa gcttccggct aacaaagcag ctgaaaatcg cgtctggccg
    13021 acaaaagccg aaaaccacct gcgctttaat atcattaatg ataaagaaaa ccgggtgaac
    13081 ggcatcgcac tctattatcg cctgccaatg gtacaagtga acgatgagca aagctttatc
    13141 gaacaagctg aatggagcat gttagttcag ctgttcaatc aacgtctgca ggaacgcata
    13201 cagtcgggcg agttgaagac tatttctggc ggcactgcgc gcagcgttaa aattgcaccc
    13261 gattatcagt cgctgttttt ccgtgtaaat gcacgagacg ataatatgca ggatgctgcg
    13321 aatgcattaa tggcagagtt ggcaaccatt gatcagcatg gtttttctgc tgaagaactc
    13381 gatgatgtca aatctacccg cctcacctgg ctgaaaaatg cggttgatca gcaagctgaa
    13441 cgtgatttac gtatgctgac cagtcgcctg gcatccagct cattaaataa tacgccgttc
    13501 ttgtcgccgg aagagacata tcaactttcg aaacgtctgt ggcagcaaat taccgtgcaa
    13561 agtctggcgg aaaaatggca gcagttaaga aagaaccagg acgcattttg ggagcaaatg
    13621 gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg cggctatcct ggcgctggaa
    13681 aaagagtacg ccaacaaaaa gctggcggct tacatcttcc caggcagaaa tttatcgtta
    13741 acagtagacg ctgacccaca ggcggaaatt agcagcaaag aaacgctggc tgagaatctg
    13801 acatcattaa cactttccaa tggtgccagg gttattctgg caaaatccgc gggtgaagag
    13861 caaaagctac aaattactgc cgtatcgaat aaaggcgatt taagtttccc tgcgcagcaa
    13921 aaatcactta tcgcgctggc aaataaagca gtaagcggaa gcggcgttgg tgagctctcc
    13981 tcttccagcc tgaaacgctg gagtgcggaa aattcggtaa ccatgagcag taaagtcagt
    14041 ggcatgaata cgttgctctc cgttagcgcg cggactaata accctgaacc tggttttcag
    14101 ttgattaacc agcgaatcac ccacagcacg attaacgaca atatttgggc atcgctacaa
    14161 aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc cagcggagaa attcgcccag
    14221 cagatgtatg agacgcgcta tgctgatgac cgcacgaaat tactgcaaga aaatcagatt
    14281 gtacagttta ctgccgcaga tgcgctggct gccgatcgcc agttgttttc atctccagcg
    14341 gatatcacgt ttgtcattgt cggtaatgtc tcagaagaca aactcgtggc gttaattacg
    14401 cgttacttag gatcaatcaa acactctgat tcgccattag ccgcaggtaa accattaact
    14461 cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa atgaacctgt ggcacaggtt
    14521 tcacagtgga agcgttatga ttcccggaca cctgttaatc tggcgacgcg tatggcgctc
    14581 gatgctttta acgtcgcact ggcaaaagat ctacgtgtta atattcgtga acaggcatct
    14641 ggagcataca gcgtttcttc tcgcctctcg gttgatcctc aggccaaaga tatcagtcat
    14701 ttgctggctt ttacttgtca accagaacga catgatgaac tgttaacgtt agcgaatgaa
    14761 gtgatggtta agcgtctggc taaagggatc agtgagcaag aactgaatga ataccagcaa
    14821 aacgttcagc gcagcctcga tatccaacag cgtagcgttc aacaattagc gaacactatt
    14881 gtaaatagtc ttattcaata tgacgatcct gcagcatgga ctgagcagga gcaattgttg
    14941 aaacaaatga cggtagagaa tgttaacact gccgttaaac aatatctttc tcatccggta
    15001 aatacttata ccggagtatt attgccaaaa taataacagc cccgtcaaca catcgttggc
    15061 ggggatttta gcaatattcg ctatttttat gtaataattt tataaatgcg ttcaaaataa
    15121 taatcaagta ctaatagtga tattttaagg tctgattttt acgtgataat tcaggagaca
    15181 cagaatgcgc ataaaaataa cagcataaaa caccttacca ccacccaaga atttcatatt
    15241 gtattgtttt tcaatgaaaa aacattattc gcgtaatatc tcacgataaa taacattagg
    15301 attttgttat ttaaacacga gtcctttgca cttgcttact ttatcgataa atcctacttt
    15361 tttaatgcga tccaatcatt ttaaggagtt taaaatggat aagaagcaag taacggattt
    15421 aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct atttccacta tcgcagaatc
    15481 aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca ttccagatta tcaatgacga
    15541 attatatctt gatggcaacg ctcgtcagaa cctggccact ttctgccaga cctgggacga
    15601 cgaaaatgtc cacaaattga tggatttatc cattaa
//
LOCUS       DAJVTY010000096        15502 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVTY010000096 DAJVTY010000000
VERSION     DAJVTY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15502
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.096"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1112
                     /locus_tag="OQQ66_002627"
     CDS             <1..1112
                     /locus_tag="OQQ66_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1905505.1"
                     /translation="AGNLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGEL
                     CDIWTKIKETELTTNTMKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETL
                     YLDNPRSNKKGRTVRTVDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPL
                     SKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILD
                     MFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVF
                     MPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIA
                     SQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            1164..2099
                     /gene="ttcA"
                     /locus_tag="OQQ66_002628"
     CDS             1164..2099
                     /gene="ttcA"
                     /locus_tag="OQQ66_002628"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1905506.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2228..3601)
                     /gene="dbpA"
                     /locus_tag="OQQ66_002629"
     CDS             complement(2228..3601)
                     /gene="dbpA"
                     /locus_tag="OQQ66_002629"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1905507.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4047..5030)
                     /gene="zntB"
                     /locus_tag="OQQ66_002630"
     CDS             complement(4047..5030)
                     /gene="zntB"
                     /locus_tag="OQQ66_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1905508.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5285..6517
                     /gene="dgcM"
                     /locus_tag="OQQ66_002631"
     CDS             5285..6517
                     /gene="dgcM"
                     /locus_tag="OQQ66_002631"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS1905509.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISSLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6538..7101)
                     /gene="smrA"
                     /locus_tag="OQQ66_002632"
     CDS             complement(6538..7101)
                     /gene="smrA"
                     /locus_tag="OQQ66_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1905510.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7431..8339)
                     /locus_tag="OQQ66_002633"
     CDS             complement(7431..8339)
                     /locus_tag="OQQ66_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1905511.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8515..9815
                     /gene="abgA"
                     /locus_tag="OQQ66_002634"
                     /pseudo
     CDS             8515..9815
                     /gene="abgA"
                     /locus_tag="OQQ66_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9815..11260
                     /gene="abgB"
                     /locus_tag="OQQ66_002635"
     CDS             9815..11260
                     /gene="abgB"
                     /locus_tag="OQQ66_002635"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS1905512.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11297..12823
                     /gene="abgT"
                     /locus_tag="OQQ66_002636"
                     /pseudo
     CDS             11297..12823
                     /gene="abgT"
                     /locus_tag="OQQ66_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12834..13349
                     /gene="ogt"
                     /locus_tag="OQQ66_002637"
     CDS             12834..13349
                     /gene="ogt"
                     /locus_tag="OQQ66_002637"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1905513.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13544..14296
                     /gene="fnr"
                     /locus_tag="OQQ66_002638"
     CDS             13544..14296
                     /gene="fnr"
                     /locus_tag="OQQ66_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1905514.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14448..15398
                     /gene="uspE"
                     /locus_tag="OQQ66_002639"
     CDS             14448..15398
                     /gene="uspE"
                     /locus_tag="OQQ66_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1905515.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
BASE COUNT         3986 a         3846 c         3830 g         3840 t
ORIGIN      
        1 aggccgggaa tttaagagcg ttgatagttc atgaaatcag ttccggtgaa tttgagtatt
       61 taagacgttt tccccagtcc agcactgggg caaaaatggt gacaacgagg gtcataaaaa
      121 cattcgggga gctttgtgat atctggacaa aaattaaaga gacagagtta acaacaaaca
      181 caatgaagaa aacgaaatca caattaaaaa cactcaggat aataatttgt gagagtaccc
      241 cgatatcgca tattcgttat agcgatatct taaactaccg gaatgaactg ctgcatggag
      301 aaacgcttta cctagataat ccaagatcca acaaaaaagg aagaaccgtg cgcacagttg
      361 atagctatat cgccctgctc tgttcgttgt tacgttttgc gtatcagtcg ggatttatat
      421 caaccaaacc atttgaagga gtaaaaaaat tacagcgaaa cagaataaaa cctgacccgt
      481 tatctaaaac agaattcaat gcattaatgg aaagtgaaaa aggacagagc caaaacttgt
      541 ggaaatttgc cgtttactcc gggcttcgtc acggggaact ggcagctctg gcgtgggagg
      601 atgtggattt cgagaaggga attgtgaatg tcagaagaaa cctgacgata cttgatatgt
      661 tcggtccccc aaaaacaaat gccgggatcc ggacggtaac attactgcag ccggctcttg
      721 aagcactgaa ggagcaatac aaactgaccg ggcatcatcg caaaagcgaa atcacttttt
      781 atcatcggga gtacggcaga actgaaaagc aaaaactgca ttttgttttc atgcccaggg
      841 tgtgtaacga aaaacagaaa ccttattact cggtaagcag tttgggtgca agatggaatg
      901 cagcagtaaa acgtgctggt attcgccgcc gtaatccgta ccatacgcga catacttttg
      961 cctgctggct gttgacggca ggagcgaacc cggcatttat agccagccag atggggcatg
     1021 aaactgcgca aatggtgtat gaaatttacg gtatgtggat tgatgacatg aacgacgaac
     1081 agatagccat gttgaatgcg cggttatcgt agttgcaaag tttgccccca atttgcccca
     1141 tttagtacca gagaactgaa ataatgcaag aaaatcaaca aattacaaag aaagaacaat
     1201 acagcctgaa caaattacaa aaacgtctac gtcgtaacgt gggcgaagcc attgctgact
     1261 tcaatatgat tgaagaaggc gatcgcatca tggtttgcct ctcagggggt aaagacagct
     1321 ataccatgct ggagattctg cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg
     1381 tggctgttaa cctcgatcaa aagcaaccgg gcttcccaga acacgttctg cccgtgtatc
     1441 ttgaaaagct gggcgttgag tacaagattg tcgaagagaa cacttacggt atcgtgaaag
     1501 agaagattcc agagggcaaa accacttgct cactgtgttc tcgccttcgt cgcggtatcc
     1561 tttatcgtac cgcaacggaa ctgggggcga cgaagatcgc gctgggtcac catcgtgacg
     1621 atatcctgca gacgttgttc ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc
     1681 cgaaactgat gagcgatgat ggcaaacata tcgttattcg tccgctggcc tactgccgcg
     1741 agaaagatat tcagcgattt gccgatgcaa aagcgttccc gattattccg tgcaacctgt
     1801 gcggttcaca gcctaacctg caacgtcagg tgattgctga catgttgcgt gactgggata
     1861 aacgttatcc agggcgtatc gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc
     1921 atctgtgcga taccaacctg ttcgatttca aaggcatcac ccacggttct gaagtggtta
     1981 acgggggtga tctggcgttt gatcgcgaag agatcccact acaaccggcg ggctggcagc
     2041 cagaagaaga tgaaaatcag ttggatgagt tacggctgaa tgtggttgaa gtgaaataac
     2101 caggatagcg cccgatgcgc aagcgtatcg ggctactctt atggaggccg gataagacgc
     2161 ggccaacgtc gcatccggca atgccgaaga agatgtttac tcttgcaccc ggcaattcaa
     2221 catttcatta ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta
     2281 actgtttcca tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat
     2341 gcacggcgat tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta
     2401 atacatcacc cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt
     2461 ctgcttccag cggcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct
     2521 gcaacatgtc agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg
     2581 ccagaccgct atttcctgca cgagctgtac ggccgatgcg atgtacatga acttcagggt
     2641 cccacgccag ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag
     2701 caacatcagt cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat
     2761 cgcgatcgcg ttgctccaaa tcgccgtgta atgacaatgc actttgccct acttcattca
     2821 gcgcgtcgca gacagcctgg caatcttttt tggtattgca aaacaccacg caagaggatg
     2881 gctgatgcaa gcttaataac cgttgcaaca gaggaatttt gcctttgctg gatgtctcat
     2941 aaaattgttg ttcaatgggt ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat
     3001 cgcgttgcac tcgtccgctg attacggcga tggcttccgg ccaggttgcc gaaaacagaa
     3061 gcgtctgtcg agatgcaggc gcaaaacgga tgacatcatc aatggcatcg ctaaatccca
     3121 tatccagcat gcggtcggcc tcatccatca ccaaagtatt cagcgcatcc agtgataccg
     3181 tgcctttttg caagtgatcc agcaaacgac ccggcgttgc cacgataata tgcggcgcat
     3241 gttgcaacga atcacgctgc ataccgaacg gttgaccacc gcacaacgtc aaaattttgg
     3301 tatttggcag aaaacgcgcc agccgacgca attcacctgc cacctgatcc gccagttcac
     3361 gcgtaggaca cagcactaaa gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca
     3421 agccgaggcc aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg cgaacatctt
     3481 ttccggcaag gatcgccgga agcgcggcgg cctgcaccgg cgtcatagtt aaataaccca
     3541 actcattaag gttcgtgagt tgggcgggag gcaaaacatt cagggtagaa aaagcggtca
     3601 caatctattc tcgtggtcat cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg
     3661 cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat
     3721 cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct
     3781 ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc
     3841 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
     3901 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caagggagat
     3961 attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc gtttttgtat
     4021 acagacagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa ccataaagca
     4081 acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa ttgccacccg
     4141 ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaaggt actgggtaaa
     4201 aagaccattg ccatcaatga cattgtatag gtacgacgag ctaaattttc ctgcatcacc
     4261 tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat ttcgtcaaga
     4321 ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac
     4381 ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc
     4441 atcacgatta attgtttgcg cagcagagcc aggaatccac gcggtggaat ttgctgatca
     4501 aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa ttcgctggaa
     4561 tgatcggtca acgcatcgca cacatccacc agccaccctc cgcaatcggt cggacccgtg
     4621 ccctcttcca gatcgctcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa
     4681 acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg gcgttcatcg
     4741 gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg gctgactcgg
     4801 ggccgtgtgc tctcgcctgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc
     4861 gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca gggatgcgct
     4921 tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag
     4981 gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac agtgcctcca
     5041 tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc
     5101 ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa gcaaaatgct
     5161 tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag aattatctga
     5221 tcatatgacg tggctttttt gcgatcggat agcaacaaaa attgataaaa ataacgggat
     5281 ctcaatgatt acgcacaact tcaataccct ggacttactc accagtcctg tctggattgt
     5341 ttcgcccttt gaggaacagt taatttatgc caatagcgcg gcgcgactgt tgatgcaaga
     5401 cctcacgttt agtcagctac gaaccggacc ctattccgtc tcctcacaaa aagaactgcc
     5461 gaaatacctc tccgatctgc aaaaccaaca cgatattatc gaaatcctca ctgttcagcg
     5521 taaagaagag gaaacagcat taagctgtcg gcttgttttg cgagagctga cagaaacaga
     5581 accggtgatt attttcgaag gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg
     5641 ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg cgcttttttc tgactaactc
     5701 tgcaccaatg ttgttgattg acccctcacg agatggccaa atcgtcgatg ctaacctcgc
     5761 cgcgctcaat ttctacggtt ataaccatga aacgatgtgc caaaaacata cctgggaaat
     5821 taatatgctc gggcgtcgcg tcatgcctat catgcatgaa atttcgcatt tacccggcgg
     5881 tcataaacct ttgaattttg ttcataaact ggcggatgga tctactcgtc atgtgcagac
     5941 ctatgccggg ccgattgaaa tttatggcga caagctcatg ttatgtattg tgcatgatat
     6001 tactgagcaa aaacggctgg aggagcagct ggaacatgct gctcaccatg acgcgatgac
     6061 cggattactg aatcggcgac agttttatca cattacggaa ccaggccaaa tgcagcacct
     6121 cgccatcgcc caggattaca gcttgttgct catcgacacc gatcgtttta aacacattaa
     6181 cgatctctat gggcattcta aaggtgatga ggtgttatgc gccctcgccc gcaccctcga
     6241 aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt
     6301 gctaccaaga accccactgg ataccgcgct ttcgctggct gaaactatcc gtgtaagcgt
     6361 ggccaaagtg agtatttcga gcttaccacg ttttaccgtc agcattggtg tggcgcatca
     6421 cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc
     6481 gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca
     6541 acggctgcgc ttagcgtggc gctcccagtt ttcttgcttc gcctgcgccg ttttacgtag
     6601 tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc gcggtgcaat atgcctgaac
     6661 atcatcaaat tcggtcagcc agcgcgccac atagctgcgg acaatattgg catgcgattt
     6721 atcttcccgc cctttaccat gaataatcag cacattacgc aaaccatccg ccagggcttg
     6781 gtgaatgaaa ctgaacacca ttttgcggca ctcttccacc ggctggcgca aaagattcag
     6841 gctcgcctgt tgcggatatt taccactgcg cagcttatcc agcaccccat gttgcaaccc
     6901 ttcccgccga aactccagcg gctgacttag cgggatgatg tcgagaaatc cggtggtgag
     6961 gaaattatca agctgcagcg tgtcgatacg ctgcggcgca cgttggttac gcgttggatg
     7021 ccagtggaca tctgtagcac gtttcagcgg ctggacatct tccatggcgt caagaaacag
     7081 cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt aagagcgcga tatgataacc
     7141 agaccgggtc ggtccaacaa cgtattacca aaaattccag taataagttc caaatattgc
     7201 cgatatttta agcaaaatgc ttatgcatga ttattcattc acgatattaa taatgtaact
     7261 tatattttcg tgaaatctgt cactgaagaa aattggcaac taaaggttaa aaccgttata
     7321 acacagtcac cggcgcagag gagacaatgc cggacttaag acgcggatgc actgctgtgt
     7381 gtactgtaga gtctggcgga tgtcgacaga ctctattttt ttatgcagtt ttaactttgc
     7441 agatagccgc attctcgccg gaattgcgtg attaatgacg gggtcagtgg tgtctggcga
     7501 ctatcacgcc gctgaatcaa ataataggcc gctttcggta aaatttcgct aaccggcaac
     7561 atcaccagcc cctgtccgtg caagggatcg cagcccattt cttcaggcag tatgctgaga
     7621 aaatcgcttt ttgccaccag actgatacag gctgagaacg tctcgcagac tacaccgacc
     7681 tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact gtttgtagta actgccgtgt
     7741 ggcgtcggca ttgtccagct gtaatccagt aactgtttga tcgaacgggc accaatggcg
     7801 gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca gtaatttctc aaaagtaaat
     7861 tcgtggtcgt acggtccctg ataataggta ttgatggtga aatccaattc tccctgacgc
     7921 aattcattaa tcatcgacac cagttgccct tccataatgc gtacttttac ctgcggatgc
     7981 tgctgatgaa aacgagatat gacagctggc atcagactgc gggaaatact ggcccccatg
     8041 ccgatattaa tctgccctgc cagttgccct tgtcgttggc gaatatcctc ttgggctgcg
     8101 cgcagctctt caagaataag actggcgtgc tgataaaaac tttcaccggc atcagttaac
     8161 gtcacgcctt tactacggcg aaaaaagagt tgcgccgcta atccttcttc caactcctga
     8221 atagatttac tcagtgccgg ttgcgacata ttcaacattc ggctcgctcc gcgaatgctg
     8281 ccctgacgag ccacttcaac aaaagcacga atttgatgaa tttttacctg aaaagccatg
     8341 acgccaccga taaccgttat ttatcagacc aaagaaactg gcatctactt taatgcagat
     8401 gattgtgtca gggtaattta tgaacggtta aaactgtgaa aaatcagtta gtgataagta
     8461 aaaactatcg ctacgtgaac cgggtcacac tttttactga tgacgggaaa ggttatggag
     8521 tctttgaatc aatttgttaa ttcgcttgcc ccaaaattat cgcactggcg acgtgatttt
     8581 catcactatg cagagtctgg ctgggtggaa ttccgcactg ccacccttgt tgcggaagaa
     8641 ttgcagcagc tcggctattc actggcgctg ggccgcgaag tagttaatga aagtagccgg
     8701 atgggactac ctgatgaact cactctacaa cgcgaattcg agcgcgctcg tcaacagggt
     8761 gcgctagcac aatggattgc ggtttttgaa ggtggtttca ctggtatcgt cgccaccctg
     8821 gataccggtc gccccggtcc ggtgatggct ttccgtgtcg atatggacgc gctggatctc
     8881 agtgaagagc aggatgtcag ccatcgcccc taccgcgacg gttttgcgtc atgtaacgcc
     8941 ggaatgatgc atgcctgtgg tcatgatgga cataccgcca ttgggcttgg gctagcgcat
     9001 acccttaaac aattcgagtc cggactacat ggcgtcatca aactgatttt tcagcctgca
     9061 gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag gtgtcgtaga tgatgttgat
     9121 tattttactg ccgtgcacat tggcactggc gtacctgcgg gcaccgtggt gtgcggcagt
     9181 gataatttta tggcaaccac caaatttgac gcgcacttca ccggtaccgc cgctcacgca
     9241 ggcgcaaaac cagaagacgg tcacaatgcc ttgttggcgg cagcacaagc cactcttgca
     9301 ctgcatgcaa tcgccccgga gcttccagag taaacgtggg cgttatgcag gcaggaagcg
     9361 gtcgtaacgt tgttcctgcc tcggcgttgc tgaaagtgga aacacgcggg gccagcgacg
     9421 tcattaatca atatgttttt gaccgtgcac aacaagcgat tcagggcgca gcaaccatgt
     9481 atggtgtcgg cgttgaaact cgtctgatgg gtgcagctac cgccagttct ccttcgccgc
     9541 aatgggtcgc atggttgcaa agtcaggcgg ctcaggtcgc ggggggcaat caggccattg
     9601 aacgtgttga agcgcctgcg ggttccgaag atgccacatt aatgatggcc cgcgtgcagc
     9661 aacatcaagg gcaagcctcc tacgtggtgt ttggcacaca gctggcggca ggtcatcaca
     9721 acgaaaaatt cgattttgac gagcaggttc tcgctattgc cgtcgaaacg ctggcgcgca
     9781 ccgcgctcaa ttttccctgg acgcgaggta tctgatgcag gaaatctatc gttttatcga
     9841 cgatgcgatt gaagccgatc gccaacgtta taccgatatt gccgatcaaa tctgggatca
     9901 tccagaaaca cgttttgaag agttctggtc agcggagcat ctggcttcgg cgctggaatc
     9961 tgcaggcttc accgttaccc gcaacgtagg ctatatccca aatgccttta ttgcttcgtt
    10021 tggtcaaggc aaaccggtta tcgccctgct gggagaatat gacgccctgg caggtttaag
    10081 tcagcaagca ggttgcgcgc aacctacatc cgtgacgccc ggtgaaaatg gtcacggttg
    10141 cggacacaat ttgctgggaa ccgccgcctt tgccgctgca atagccgtca agaaatggct
    10201 ggaacaatat gggcaaggcg gcacggtgcg cttttatggt tgtcctggcg aagaaggcgg
    10261 ctcgggtaaa acgttcatgg ttcgcgaggg ggtatttgat gatgtggatg cggcactcac
    10321 ttggcacccg gaagcctttg ccggtatgtt caatacccgc acgctggcaa acattcaggc
    10381 atcatggcgc tttaaaggga tcgcagcaca tgccgcgaat ttccctcatt tgggacgcag
    10441 cgcccttgat gccgtaacgt tgatgaccac tggcaccaac ttcctcaacg aacatattat
    10501 tgaaaaagcg cgcgtacact atgccatcac aaatagcggc gggatctcgc ccaacgtggt
    10561 ccaggcgcag gcagaagtgc tttatcttat ccgcgccccc gaaatgaccg acgtgcagca
    10621 tatttatgat cgggtcgcca aaatcgccga aggtgcggca ttgatgaccg aaaccacggt
    10681 tgaatgccgc ttcgacaaag cctgttccag ttatctcccg aatcgcacct tagaaaatgc
    10741 catgtaccat gccctatccc attttggtac cccggaatgg aactccgaag aactggcttt
    10801 tacgaaacaa attcaggcta cgctcccccc caacgatcgg caaaacagtc tgaataatat
    10861 cgccgcaacc ggtggcgaaa acggcaaggc ttttgcacta cgtcatcgtg aaacggtact
    10921 ggcgaatgag gtcgctccat atgccgccac cgataacgtg cttgcggcat cgactgatgt
    10981 cggcgacgtc agttggaaac tgcctgttgc ccagtgtttc agcccctgtt ttgccgtcgg
    11041 tacaccgcta catacgtggc aactggttag ccaggggcga acatctattg ctcataaagg
    11101 aatgctgctg gcggcgaaaa ctatggcagc aaccacactc aatctcttca ttgattcagg
    11161 gctattgcaa gaatgccaac aagagcatca gcaagttacg gacacgcaac cgtatcactg
    11221 ccctatccca aaaaacgtga cactgtcatc tttaaaataa caacaacaac gcaaacacaa
    11281 caaccgagga atgcccatga gtatgtcatc cataccgtcg tcctcctaat ccgggaagct
    11341 ctatggctgg gtcgaaagaa ttggtaacaa ggttccccat ccttttttgc tctttatcta
    11401 tttgattatc gtactcatgg tgacgacggc aattttgtcg gcctttggcg tcagtgcgaa
    11461 aaacccgacc gatggaacgc cggtggtggt gaaaaacctg ctcagtgtgg aaggattaca
    11521 ctggttttta cccaatgtta ttaaaaactt tagcggtttt gctccacttg gtgcgatcct
    11581 ggcgctggtt ttaggtgccg gtctggcgga gcgcgtcggc ttactgccag cgctaatggt
    11641 taaaatggca tcgcatgtta atgtccgcta cgccagttat atggtgctgt ttattgcttt
    11701 tttcagccac atttcttccg atgcggcgtt agtgatcatg ccaccgatgg gtgcgctgat
    11761 ttttctggcg gtgggcaggc atccagttgc aggtttactg gctgccattg caggcgtagg
    11821 ttgcggcttt acggctaatt tactgattgt cacaaccgac gtgttgctgt cggggatccg
    11881 cacggaagcg gcggctgcgt tcaatccgca aatgcacgtc agtgtaattg ataactggta
    11941 ttttatggcc agctccgtag tcgtactgac gattgttggc ggcctgataa ccgacaaaat
    12001 catcgagcca cggttaggtc aatggcaggg aaacagcgat gagaaactgc agacattgac
    12061 cgaaagtcag cgttttggtt tacgcatagc aggtgtcgta tcgctacttt ttattgctgc
    12121 gatcgcgctg atggtgatcc cggaaaacgg gatattgcgc gatccgatta atcacaccgt
    12181 gatgccatca ccctttatta aaggtatcgt gccactaatc attctttttt tctttgtggt
    12241 ttcgctggct tatggcatcg ctacccgcac aattcgacat caggcggatt taccgcattt
    12301 aatgattgaa ccgatgaaag agatggcggg atttatcgtg atggtttttc ccctcgccca
    12361 gtttgtcgcc atgtttaact ggagcaacat ggggaaattc atcgccgtgg ggctgaccga
    12421 tatactggaa agttcagggc ttagcggcat cccggcgttt gtcggtctgg cgttgctttc
    12481 ctctttctta tgcatgttta ttgccagtgg ttccgcaatc tggtcgattc tggcccccat
    12541 tttcgtacca atgtttatgc tacttggctt tcacccggca tttgcgcaaa tcctctttcg
    12601 tattgccgac tcatccgtat tgcctttagc gccggtatct ccttttgttc cactgtttct
    12661 tggattcctg caacgctaca aaccagacgc gaaactgggt acttactatt cgttagtctt
    12721 gccctatccg cttatctttt tggtggtatg gctgctgatg ttgctggcgt ggtatcttgt
    12781 tggtctgccg ataggtccgg ggatttaccc acgtttgtct taagagagaa cggatgctga
    12841 gattacttga agaaaaaatt gccacgccac tgggtccact gtgggtgatt tgcgatgagc
    12901 aatttcgcct gcgggcggtt gaatgggaag agtacagcga acgcatggtg cagctgctgg
    12961 acatccatta tcgcaaagaa ggctatgagc gcatttctgc caccaaccca ggcggtttaa
    13021 gcgacaagct tagtgattat tttgccggta atcttagcat tattgatacg cttcccactg
    13081 ccacaggggg gacgccattt cagcgcgaag tctggaaaac tctacgcact atcccctgcg
    13141 ggcaggtaat gcattacggc caactggctg aacaattggg ccgccctggc gcggcgcgtg
    13201 ccgttggtgc ggcaaacgga tcgaatccca tcagcatcgt cgtaccttgc catcgggtta
    13261 ttggccgaaa cggcaccatg accggatatg caggcggagt tcagcgaaaa gagtggttat
    13321 tgcgccacga aggttatctt ttgctgtaaa cattaaacaa tttgtgccag cttgttcaca
    13381 cttttatgta aagttaccct taacaactta atggttttca aatagataga catatattta
    13441 catctaatat cggaattctc tgctgttaag gtttgcttag acttacttgc tccctaaaaa
    13501 gatgttaaaa ttgacaaata tcaattacgg cttgagcaga cctatgatcc cggaaaagcg
    13561 aattatacgg cgcattcagt ctggcggttg tgctatccat tgccaggatt gcagcatcag
    13621 ccagctttgc atcccgttca cactcaacga acatgagctt gatcagcttg ataatatcat
    13681 tgagcggaag aagcctattc agaaaggcca gacgctgttt aaggctggtg atgaacttaa
    13741 atcgctttat gccatccgct ccggtacgat taaaagttat accatcactg agcaaggcga
    13801 cgagcaaatc actggtttcc atttagctgg cgacctggtg ggatttgacg ccatcggcag
    13861 cggccatcac ccgagcttcg ctcaggcgct ggaaacctcg atggtatgtg aaatcccgtt
    13921 cgaaacgctg gacgatttgt ccggtaaaat gccgaatctg cgtcagcaga tgatgcgtct
    13981 gatgagcggt gaaatcaaag gcgatcagga catgatcctg ctgttgtcga agaaaaatgc
    14041 cgaggaacgt ctggctgcat tcatctacaa cctgtcccgt cgttttgccc aacgcggctt
    14101 ctcccctcgt gaattccgcc tgacgatgac tcgtggcgat atcggtaact atctgggcct
    14161 gacggtagaa accatcagcc gtctgctagg tcgcttccag aaaagcggta tgctggcagt
    14221 caaaggtaaa tacatcacca tcgaaaataa cgatgcgctg gcccagcttg ctggtcatac
    14281 gcgtaatgtt gcctgatttt tccgcataac tcactatcct tctgtcatat cattaaattt
    14341 ttctgattta ttgatctggc agaaggttca tcactgtttc attcaccaga tatgggttaa
    14401 tcttttaatt acaaactgcg ttgacagttg ttgtaaggag accctgtatg gctatgtatc
    14461 agaacatgct cgttgttatc gatcctaacc aggacgacca accagcattg cggcgagctg
    14521 tttatttaca tcaacggatt ggtggcaaaa ttaaagcctt tttgccgatc tatgacttct
    14581 catacgaaat gaccaccctg ctctccccgg acgaacgtac cgctatgcgt cagggcgtca
    14641 tcagccagcg tacagcctgg atccacgagc aggcaaaata ttatctcaat gctggcgttc
    14701 ccattgaaat taaagtggtc tggcataacc gtcctttcga agccatcatt caggaagtga
    14761 tcagcggcgg gcacgatttg gtgctaaaaa tggcgcacca acatgaccgt ctggaagcgg
    14821 tgatttttac gccaacggac tggcatctgt tacgcaaatg cccaagcccg gtgtggatgg
    14881 tgaaagacca gccgtggccg gaaggaggta aggcgctggt ggcggtgaat ctcgccagtg
    14941 aagagccgta tcataatgcg ctcaatgaaa aactggtcaa agagacgatc gaactggcag
    15001 aacaagtcaa ccataccgaa gttcatctgg ttggcgctta cccagtaacg ccaattaata
    15061 tcgcgattga actgccggaa tttgagccga gcgtttataa cgatgccatt cgtgggcaac
    15121 atttgctggc aatgaaagcc ctgcggcaga aattcggcat taatgaaaac atgacgcacg
    15181 tagaaaaagg tctgcccgaa gaggttattc ctgatttggc ggagcactta caggcgggga
    15241 ttgtggttct gggcacggta ggacgcaccg gtatttcagc agcattcctc ggcaacacgg
    15301 cggaacaggt gattgatcat cttcgctgcg atctgttggt cattaaacct gaccagtatc
    15361 agacacccgt tgaactggat gacgaagaag acgattaacg actcttcaat aactgacaag
    15421 gcccgagtta tcgggcctat tttttactta ctgctcttcg ccgccaagga aataaattcc
    15481 caacgggatt gccagcaaca ca
//
LOCUS       DAJVTY010000097        15112 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVTY010000097 DAJVTY010000000
VERSION     DAJVTY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15112
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.097"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..423
                     /locus_tag="OQQ66_002640"
     CDS             <1..423
                     /locus_tag="OQQ66_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1905516.1"
                     /translation="TLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKR
                     LAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMT
                     LSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            439..921
                     /locus_tag="OQQ66_002641"
     CDS             439..921
                     /locus_tag="OQQ66_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1905517.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(998..1345)
                     /gene="rplS"
                     /locus_tag="OQQ66_002642"
     CDS             complement(998..1345)
                     /gene="rplS"
                     /locus_tag="OQQ66_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1905518.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1387..2154)
                     /gene="trmD"
                     /locus_tag="OQQ66_002643"
     CDS             complement(1387..2154)
                     /gene="trmD"
                     /locus_tag="OQQ66_002643"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1905519.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2185..2733)
                     /gene="rimM"
                     /locus_tag="OQQ66_002644"
     CDS             complement(2185..2733)
                     /gene="rimM"
                     /locus_tag="OQQ66_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1905520.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2752..3000)
                     /gene="rpsP"
                     /locus_tag="OQQ66_002645"
     CDS             complement(2752..3000)
                     /gene="rpsP"
                     /locus_tag="OQQ66_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1905521.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3137..4498)
                     /gene="ffh"
                     /locus_tag="OQQ66_002646"
     CDS             complement(3137..4498)
                     /gene="ffh"
                     /locus_tag="OQQ66_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1905522.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4665..5456
                     /locus_tag="OQQ66_002647"
     CDS             4665..5456
                     /locus_tag="OQQ66_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1905523.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5477..6763
                     /locus_tag="OQQ66_002648"
     CDS             5477..6763
                     /locus_tag="OQQ66_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1905524.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6818..7411)
                     /gene="grpE"
                     /locus_tag="OQQ66_002649"
     CDS             complement(6818..7411)
                     /gene="grpE"
                     /locus_tag="OQQ66_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1905525.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7534..8412
                     /gene="nadK"
                     /locus_tag="OQQ66_002650"
     CDS             7534..8412
                     /gene="nadK"
                     /locus_tag="OQQ66_002650"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1905526.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8498..10159
                     /gene="recN"
                     /locus_tag="OQQ66_002651"
     CDS             8498..10159
                     /gene="recN"
                     /locus_tag="OQQ66_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1905527.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10308..10649
                     /gene="bamE"
                     /locus_tag="OQQ66_002652"
     CDS             10308..10649
                     /gene="bamE"
                     /locus_tag="OQQ66_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1905528.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10711..11001)
                     /locus_tag="OQQ66_002653"
     CDS             complement(10711..11001)
                     /locus_tag="OQQ66_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1905529.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10991..11428)
                     /gene="ratA"
                     /locus_tag="OQQ66_002654"
     CDS             complement(10991..11428)
                     /gene="ratA"
                     /locus_tag="OQQ66_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1905530.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11599..12081
                     /gene="smpB"
                     /locus_tag="OQQ66_002655"
     CDS             11599..12081
                     /gene="smpB"
                     /locus_tag="OQQ66_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1905531.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12296..12658
                     /gene="ssrA"
                     /locus_tag="OQQ66_002656"
     tmRNA           12296..12658
                     /gene="ssrA"
                     /locus_tag="OQQ66_002656"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12819..15112)
                     /locus_tag="OQQ66_002657"
                     /pseudo
     CDS             complement(12819..15112)
                     /locus_tag="OQQ66_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3705 a         4058 c         3544 g         3805 t
ORIGIN      
        1 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
       61 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagggt cttgattgaa
      121 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
      181 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca acagatatgc
      241 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
      301 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
      361 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
      421 taacaaggat atatcgatat gataaagcac ctggtagccc ccctgatttt cacctcacta
      481 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
      541 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
      601 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
      661 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
      721 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
      781 gcatgggcta ttggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa
      841 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
      901 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
      961 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
     1021 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
     1081 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
     1141 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
     1201 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
     1261 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
     1321 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
     1381 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
     1441 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
     1501 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
     1561 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
     1621 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
     1681 ccccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
     1741 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
     1801 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca attcgctgac
     1861 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
     1921 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
     1981 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
     2041 aggactccag ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
     2101 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
     2161 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
     2221 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
     2281 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
     2341 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
     2401 caggtctttc cagtagtagt caccctcttc aagctgaggc aactgcgatg aatccacgac
     2461 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
     2521 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
     2581 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
     2641 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
     2701 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
     2761 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
     2821 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
     2881 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
     2941 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
     3001 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
     3061 attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag ttaataactc
     3121 tgtcgcataa ggtcatttag cgaccaggaa atcccggcgg catcatcccc ttcatgcttc
     3181 tcatcatctt cgccattccg cccttcttca ttttcttcat catgcgctgc atgtcgtcga
     3241 actgtttcag aagacggtta acgtcctgca cctgcatacc gcaaccggca gcaatacggc
     3301 gtttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
     3361 tgatggcttc catacgcacc agcactttat cgtccatctg tgacttgacg ttatccggga
     3421 tctgccccat gcccggcagc ttgcccatca ggctggccat gccgcccata tttttcatct
     3481 ggcgcagctg ctcaagaaag tcgttgagat cgaagccgtc accttttttc agcttgctgg
     3541 ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagta
     3601 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
     3661 cagttttctc gccaacaccg aggaacttga tcggtttgcc agtgatgtga cgaatagaga
     3721 gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
     3781 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
     3841 caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg tccatcatcg
     3901 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
     3961 tggcttcttt cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga
     4021 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
     4081 aaacgtcggc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
     4141 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc cgccatcagc acgaccgcag
     4201 gcggttgcgc cgccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
     4261 tacggacgat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
     4321 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acatccgcct
     4381 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gttaggcgtc
     4441 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
     4501 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
     4561 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
     4621 ctatactgct tctctttctt attgctcaaa ctgttgacat cactatgccc gtttttgctc
     4681 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
     4741 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
     4801 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
     4861 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
     4921 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
     4981 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
     5041 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
     5101 tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt aaccaggaaa
     5161 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
     5221 tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt agcatggaaa
     5281 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
     5341 gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggcgcgg
     5401 taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc agctaaaccc
     5461 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
     5521 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
     5581 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
     5641 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
     5701 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
     5761 aattgcgact ggtgtgctga cttttgtcgt actggtattt gctgaggtat tgccgaaaac
     5821 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
     5881 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
     5941 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
     6001 cactatcgtg cacgaatcac gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
     6061 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
     6121 cggtattgat atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca
     6181 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
     6241 tgaagcctgg cgattgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
     6301 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtaaagtt
     6361 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
     6421 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
     6481 aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg atggcaccgc
     6541 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
     6601 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
     6661 tattggcgag tacgatatag atattctcga cgtacaggac aatatgatta agcaggtaaa
     6721 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggccctgcat
     6781 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
     6841 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
     6901 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
     6961 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
     7021 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
     7081 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
     7141 atttctccag cgcaaatttg tgggcttttt caatatccag ttcagtacgt cgacgcaggt
     7201 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
     7261 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
     7321 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
     7381 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
     7441 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
     7501 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
     7561 attgtgggac acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg
     7621 ctgtgcaaaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
     7681 aagaatgtga aaactggcac gctcgcggag attgggcaac tagctgatct cgcggtagtc
     7741 gttggtggcg acggtaatat gctgggcgcg gcacgcacac tcgcccgtta cgatattaaa
     7801 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
     7861 cagcaacagt tagccgatgt actggaaggc cactacatca gcgagaaacg ttttttgctg
     7921 gaagcgcaag tctgtcagca agattgccag aaacgcatca gcaccgcgat aaatgaagtg
     7981 gtgcttcatc caggcaaagt ggcacatatg attgagttcg aagtgtatat cgacgagatc
     8041 tttgcgtttt ctcagcgatc tgatggacta attatttcga cgccaacagg ctccaccgcc
     8101 tattccctct ctgcaggcgg tcctattctg accccctctc tggatgcgat taccctggtg
     8161 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
     8221 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
     8281 ctgccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
     8341 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
     8401 aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt atactgtacg
     8461 caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac tgaccatcag
     8521 caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac
     8581 tggcgagacc ggcgcgggta aatctattgc aatagatgcc ctcggtcttt gtctcggtgg
     8641 tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt gcgcccgttt
     8701 ttctctgaaa gatacgccag cggctctgcg ctggctggaa gaaaatcagc ttgaagacgg
     8761 gcatgaatgt ttgcttcgtc gcgtaatcag cagcgatggt cgctcccgtg gtttcatcaa
     8821 cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca
     8881 tggtcagcac gctcatcaat tactcaccaa acctgagcac caaaaattcc tgcttgatgg
     8941 ctatgccaat gaaacctctc tactgcagga aatgaccgca cgttatcagt tgtggcatca
     9001 aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgccg cccgtgcgga
     9061 actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg gagagtttga
     9121 acaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca
     9181 gaatgcattg gcattaatgg cctacggtga agacgcaaac ctgcaaagtc agctttacac
     9241 ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga
     9301 tatgctggaa gaagctacca tccagattgc tgaagccagc gatgaactgc gccactactg
     9361 cgatcgtctg gatctcgatc ccaaccgact atttgaactt gaacagcgca tctcaaaaca
     9421 gatttcgctg gcacgtaaac atcacgtcag ccctgaggca ttgccacagt attaccagtc
     9481 gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa cgcttgcgct
     9541 ggcggtaacg aaacatcatc agcaggcact ggaaatcgcg cgcgcattac accaacaacg
     9601 ccagcaatat gcagaagaac ttgcacagct gatcaccgac agtatgcatg cgctctcaat
     9661 gccgcatggg cagtttacga tcgatgttaa atttgacgag catcacctgg gcgctgacgg
     9721 tgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc
     9781 caaagtcgca tccggtggtg aattgtcccg catcgcactg gcaacccagg tcatcacggc
     9841 gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggtcc
     9901 aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc aggtgatgtg
     9961 tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga
    10021 aaccgatggt gcgatgacag aaacgcatat gcaatccctg aataaaaaag cgcggttaca
    10081 agagctggcg cgcctgcttg gtggcagtga agtcacacgt aatacactgg cgaatgcgaa
    10141 agaactgctt gcagcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc
    10201 cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt
    10261 acagatgagc cacgtactgc tagggcccga aaaggaatca aatcactatg cgctgtaaaa
    10321 cgctgactgc tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc
    10381 gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca
    10441 aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt
    10501 ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca ggtcatgaag
    10561 gtgtaacgca gcaaacgctg acgctgacct ttaacagtag cggtgtgttg accaatattg
    10621 ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa
    10681 ggtgctcaat gagcaccttt tttctgtctg ttatttattc gctgattttt ctgctcgttg
    10741 cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc gatcgccatc
    10801 atgcacacta tcgcttagtt ttgccggacg gctgtaaatg ccgactttgt ttttagttaa
    10861 atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt caaccgtcgc
    10921 gccctcctgc agcgtcacgc gctgcaggta ctgcttctca ggtagcgcat aagccacctc
    10981 aacggcaatt ttacctggca ctgtaaacct ctttcgcacg aaccgtaaaa gcctggacca
    11041 tattagacgc cagctcttta aacacgcgac caaaggcgag ttcaatcaac ttattggtaa
    11101 actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc gtaaacttcc
    11161 atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata ctttggttac
    11221 tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag acatctaccg
    11281 ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa
    11341 actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta
    11401 ccagtgcggt ccggctaatc tgaggcataa ctatttccat caacaaaaat ccaacaaata
    11461 atatcattta tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca tcgggttcat
    11521 gctaagatag agccttgtcc cccgcaggat tgatatgggg tgttttcgat ttcagattac
    11581 cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc
    11641 gcgcttaaca agcgcgcccg tcacgaatac tttatcgaag aagagttcga agcgggactt
    11701 gccctgcaag gctgggaagt taaatccctg cgcgcaggaa aagccaatat cagcgacagc
    11761 tacgtccttc tgcgtgacgg agaggcattt ctgtttggcg ctaacatcac gccaatggcc
    11821 gtggcctcca cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag
    11881 cgcgaactgg actcattgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc
    11941 tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaaaaaa
    12001 cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcacgtatc
    12061 atgaaaaacg cccaccgtta aacctgcact ccaattattg accagttcct caccgcgcct
    12121 ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacac aaatgttgcc
    12181 atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt taacgataac
    12241 gccattgagg ctggtcatgg cgctcataaa tctggtatac ttgccgttac acattggggc
    12301 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
    12361 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
    12421 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
    12481 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
    12541 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
    12601 tgtagtaccg aggacgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccaat
    12661 catgattgga cggtataagg acaacaccaa taaaaacagg aggttagcag tctcagcagg
    12721 acaccgacca gacggtgaag agacataaaa ggatacgcaa aggagccgcg gctcctggtg
    12781 acatgaaagc ccacagatgt gggctttttc gttgatggtc agaacgacca gttcacacca
    12841 gctaccccgt tccacgggga ttccacaccg gcaccatggc tataccccac cccaagatgc
    12901 ccgcttaacg tactgctgaa tgaggcttta atacctgcct ggtatattcc acgtctgccc
    12961 gacaaatcat tgacgaaatt accgtcacta ttcactttca cccggttatc atcgacaaat
    13021 tctttgcgca cagccgcctt cagccacggc tcaacttcca taccgttccc cagacgcatg
    13081 ttgtaactca gcgttgcacc cagttcacga tataaacctg cgggtatcga ctgatttcga
    13141 ttccatgcca ttggataaat gatattcagg gttatcagcg gtgaaccccc gttaacgatg
    13201 catacggcgt caggttccag ttaccatcgg taaatcgcat cccggtttca atgtgaccgc
    13261 ccagcccgtt gctgcgataa ctgccgttgg tggctccacc gctgccaacc gtggagtcgc
    13321 gagcttcggt acctgctaat accaataaat taccgccatt ttccagcaac atattggtcg
    13381 ctaaattgcc ggaaatggaa aaagtgccgt actggtgagt accgctgatt tcaataccgt
    13441 tagccgtgct cgtctggaga gcggcaccgc tgttctggac gatatctgtc gctttgccat
    13501 tatcgttaac tgtcagcgta ccgccttcat tgatctttgt ttttattgcc tctccgttag
    13561 ctgaaactgt ttgtattccg ccgtcgttaa tcgttgtctc attcgccacc ccctcgacga
    13621 tttgttcgcc gccggtgagc gtcgtgcctg tcgcagtggc ttttgttttg acgatctccc
    13681 gtccgcccgt attgacctgt gttttgtcag aagaggtgtc tgactccacg gttaacacgc
    13741 cgccatttgc cagcaggata ttgttcgccg caccctgctc gatgctgaac gcgacgccat
    13801 ccgcgcgtgt tcctgtgacc cgcgtcgccc tggtggttgc aaccaaagcg ccctggctac
    13861 tctgctgtat ccccgttgcg ctgcctttct cccgcacatc gagtgtgccg ccgtcattaa
    13921 gcaccgagtt ttcagccaga ccgccctcat taactacctg tgaaccccca ttaataatgg
    13981 aaccttccgc tgtcccgttt gccataattt gttgtaggcc agagacgata tcggtattga
    14041 ttgccttacc ataattctga acggtttgcg tgccaccatt gatgtgtgtt ttctctgttg
    14101 acccaccatc aacaatttgt tcaccacttt cgatatttgc ctcagtggct aatccatata
    14161 ccgtttgctt gccacctttg atatttgctt tatcagaagt ggcactggca tatattgttt
    14221 gggtgccagc actattgagt acagtgccaa catcttttcc ataaacatcc attttgccgt
    14281 tggcattaat aatcgtatca actgcccggg aaccagtgac gactgttaat gagccagcgt
    14341 tttccagcac tacattttta gcttctgaat tcctgatgta gaaagcatca ccataactgt
    14401 tggttccttt gataagggtt ccggaagttg tggaagcaat taatgcgccg ccggattgtt
    14461 gctcaacatg cttagcctca ccaccgtcct gaacctccag aacgccacca ttattaagtc
    14521 tggtggtatc tgttttagcc tccttctgga caatcagctt accgccagca tcaacggtag
    14581 tatttttcgc cgaggtttta gccactaccg tcagttcgcc ggtattttcc agcacaacat
    14641 aattagcctc ccctccgggt aatagtgaag tgagagagct tgttgtatcc ttcgatatca
    14701 gtccctgcgc ccgtgttggc aactaaagca ctgcccgtct cctggttaac cccatgtgct
    14761 ataccgccgg tatagacaat cagcgagcct ccggcgctaa tattgctgcc agttgccgta
    14821 ccatctttct caacaacctg ccggctcccg gtggaaatat ttgtcgtgtc agctttcccg
    14881 ccgctcttga tattttgtgt tccgctgttg atattggtac ccgtggcaat accatgatta
    14941 ttaatattct gtgtaccacc attgattatg gtattggtcg cgtttccggt aacatccatt
    15001 accccgccgt tatctattcg ggtcgcatca gctttagcat tcgttaaaac tgacattgtt
    15061 cctttatctt taataatcgt cttatttgcc gaaccatatg cgtttatgtc ta
//
LOCUS       DAJVTY010000098        14953 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVTY010000098 DAJVTY010000000
VERSION     DAJVTY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.098"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(37..327)
                     /locus_tag="OQQ66_002658"
     CDS             complement(37..327)
                     /locus_tag="OQQ66_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1905532.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            complement(586..1467)
                     /gene="yddG"
                     /locus_tag="OQQ66_002659"
     CDS             complement(586..1467)
                     /gene="yddG"
                     /locus_tag="OQQ66_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1905533.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTSVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1699..4746
                     /gene="fdnG"
                     /locus_tag="OQQ66_002660"
     CDS             1699..4746
                     /gene="fdnG"
                     /locus_tag="OQQ66_002660"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2284..2286,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1905534.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4759..5643
                     /gene="fdnH"
                     /locus_tag="OQQ66_002661"
     CDS             4759..5643
                     /gene="fdnH"
                     /locus_tag="OQQ66_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1905535.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5636..6289
                     /gene="fdnI"
                     /locus_tag="OQQ66_002662"
     CDS             5636..6289
                     /gene="fdnI"
                     /locus_tag="OQQ66_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1905536.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6340..6624)
                     /locus_tag="OQQ66_002663"
     CDS             complement(6340..6624)
                     /locus_tag="OQQ66_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1905537.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6770..7780)
                     /gene="adhP"
                     /locus_tag="OQQ66_002664"
                     /pseudo
     CDS             complement(6770..7780)
                     /gene="adhP"
                     /locus_tag="OQQ66_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7914..9611)
                     /gene="maeA"
                     /locus_tag="OQQ66_002665"
     CDS             complement(7914..9611)
                     /gene="maeA"
                     /locus_tag="OQQ66_002665"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1905538.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9768..9905)
                     /gene="sra"
                     /locus_tag="OQQ66_002666"
     CDS             complement(9768..9905)
                     /gene="sra"
                     /locus_tag="OQQ66_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS1905539.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(10007..10222)
                     /gene="bdm"
                     /locus_tag="OQQ66_002667"
     CDS             complement(10007..10222)
                     /gene="bdm"
                     /locus_tag="OQQ66_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS1905540.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10568..10999
                     /gene="osmC"
                     /locus_tag="OQQ66_002668"
     CDS             10568..10999
                     /gene="osmC"
                     /locus_tag="OQQ66_002668"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1905541.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(11055..11981)
                     /locus_tag="OQQ66_002669"
     CDS             complement(11055..11981)
                     /locus_tag="OQQ66_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1905542.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11974..12960)
                     /locus_tag="OQQ66_002670"
     CDS             complement(11974..12960)
                     /locus_tag="OQQ66_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1905543.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12957..13853)
                     /locus_tag="OQQ66_002671"
     CDS             complement(12957..13853)
                     /locus_tag="OQQ66_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1905544.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLIWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13850..14872)
                     /locus_tag="OQQ66_002672"
     CDS             complement(13850..14872)
                     /locus_tag="OQQ66_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1905545.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
BASE COUNT         3732 a         3842 c         3829 g         3550 t
ORIGIN      
        1 ggtcagtgat catattgtga tgggcactta actcaatcag agattcttgc cgcgcgagcc
       61 gttggcttca tcgttatagc ctttaaaatc atattcccag tgaccaaaac caatcatttg
      121 atcattgatt tgcgtttctc ctttaaagcc gagacgggca taagttttat caccatcgtc
      181 acgcttatca tcagtaaaat aacgtagagc ggtaaccttg ccataaaggt cgagtttatt
      241 accatccttg ttatagactt cggcagcgtg tgctacgttc gcagctaaca aaccagttac
      301 aaccaccgca actattttta atttcattta acagaatcct tttaattatc gtcaaacgta
      361 ttttctaaac gaattttaaa cggcgtcatt tataaatgac atactgtttt taaaatagaa
      421 tggtgattaa aaacaatgag aaaaatagat gagataatat tatttatcga tatgtgatcg
      481 aagtcgaaat gagatataag gtgaattact ggtatttgaa atttattttt taatattgtc
      541 ggaatttatc tgattaactg ccgggccgta gacccggcaa ttattttaac cacgacgtgt
      601 cgccagccag cagagcaggg aaccgccgca gaccattagc gcgccttgcc agaatgagaa
      661 cgacagcggg gcgctgagca gcacggctgc aagcgctgag gaaagtacgg gcgtaaaata
      721 cgagcctacc gccattatgg tgacattgcc atgcaatata ccgacattcc atgcagcata
      781 agcaaatcct aaggtaaatg ccgcagagat gagtttaatc atgacggacg tgctaaatac
      841 catttctggt tgttgcgtaa gaaaatagta aacccacaga cttgctcccg ttagcaggac
      901 aaaaacggta attccgttaa atccgcgtgc gtatttattc gttactgtgc aataggctgc
      961 ccagataaac gcaccaataa acgccaggaa ataactcaat gggctggtgg tgatattatt
     1021 gatgatttca tcataatgta acccattctc accgcctaac acccagcaga cgccgaccag
     1081 ggctaataat aatcctggta caatcagcca gttggttttc tgaccattaa acagaatggc
     1141 aaagagaatt gtcaggctgg gccacagata gttcaccata cccgcttcaa tcgcctgatg
     1201 acgggtcgcc gcatacccta aggaaagcgc cagacagatt tcatagctga cgaataacag
     1261 actcccggcg agtaaatagc ctttcggaat ctgccgaata cgcggaaatc caaccgtgaa
     1321 gattaacagc agcccgctta atgaatagat agcagctgcg ccgccgaccg ggccgagccc
     1381 ctcgctgaca ccgcgaatca atcctaccat cgtgctccac aggacgatcg ctattagccc
     1441 tatgagcgtt gctttttgtc gtgtcatgct cgctgttttg tctctcttgc cgttaaaaat
     1501 taagctgaat tttatagcat ttttttaact ggcctgtcag gcagtggtgc gtttttctac
     1561 cgctattgag gtaggtcaat ttgcgcaggc ggattatttt gtggcaaaca cgtgttcttt
     1621 ttgatttcgc gcaaaaagat tcagaatttt actgttagtt tcctcgcgca gtaatatccc
     1681 tgaaaaaaga ggaaagcaat ggacgtcagt cgcagacaat tttttaaaat ctgcgcgggc
     1741 ggtatggctg gaacaacggt agcggcattg ggctttgccc ctaagcaagc actggctcag
     1801 gcgcgaaact acaaattatt acgcgctaaa gagatccgta acacctgcac atactgttcc
     1861 gtaggttgcg ggctattgat gtatagcctg ggtgatggcg cgaaaaacgc cagagaagcg
     1921 atttatcaca ttgaaggtga cccggatcat ccggtaagcc gtggtgcgct gtgcccaaaa
     1981 ggggccggtt tgctggatta cgtcaacagc gaaaaccgtc tgcgctaccc ggaatatcgt
     2041 gcgccaggtt ctgacaaatg gcagcgcatt agctgggaag aagcattctc ccgtattgca
     2101 aagctgatga aagctgaccg tgacgctaac tttattgaaa agaacgagca gggcgtaacg
     2161 gtaaaccgtt ggctttctac cggtatgctg tgtgcctccg gtgccagcaa cgaaaccggg
     2221 atgctgacac agaaatttgc ccgctccctc gggatgctgg cggtagacaa ccaggcgcgc
     2281 gtctgacacg gaccaacggt agcaagtctt gctccaacat ttggtcgcgg tgcgatgacc
     2341 aaccactggg tggatatcaa aaacgctaac gtcgtaatgg taatgggcgg taacgctgct
     2401 gaagcgcatc ccgtcggttt ccgctgggcg atggaagcga aaaacaacaa cgatgcaacc
     2461 ttgatcgctg tcgatcctcg ttttacgcgt accgcttctg tggcggatat ttacgcacct
     2521 attcgttccg gtacggacat tacgttcctg tctggcgttt tgcgctacct gatcgaaaac
     2581 aacaaaatca acgccgaata cgttaaacat tacaccaacg ccagcctgct ggtgcgtgat
     2641 gattttgctt tcgaagacgg cctgttcagc ggctacgacg ctgaaaaacg ccagtatgat
     2701 aagtcgtcct ggaactatca gttcgatgaa aacggctatg caaaacgcga tgaaacactg
     2761 actcatccgc gctgtgtgtg gaacctgctg aaagcgcacg tttcccgcta cacgccggac
     2821 gtcgttgaaa acatctgcgg tacgccaaaa gccgacttcc tgaaagtgtg tgaagtgctg
     2881 gcctccacca gcgcaccgga tcgcacaacc accttcctgt acgcgctggg ctggacgcag
     2941 cacaccgtgg gtgcgcagaa catccgtact atggcgatga tccagttgct gctcggtaac
     3001 atgggtatgg ccggtggcgg cgtgaacgca ttgcgtggtc actccaacat tcaggggctg
     3061 actgacttag gtctgctctc taccagcctg ccaggttatc tgacgcttcc gtcagaaaaa
     3121 caggttgatt tgcagtcgta tctggaagcg aacacgccga aagcgacgct ggctggtcag
     3181 gtgaactact ggagcaacta tccgaagttc ttcgttagcc tgatgaaatc tttctatggc
     3241 gatgccgcgc agaaagagaa caactggggc tatgactggc tgccgaagtg ggaccagacc
     3301 tacgacgtca tcaagtattt caacatgatg gacgaaggca aagtcaccgg ttatttctgc
     3361 cagggcttta acccggttgc gtccttcccg gacaaaaaca aagtggtgag ctgcctgagc
     3421 aagctgaagt acatggtggt tatcgatccg ctggtgactg aaacctctac cttctggcag
     3481 aaccacggcg agtcgaacga tgtcgatccg gcgtctattc agactgaagt attccgtctg
     3541 ccttcgacct gctttgccga agaagatggt tctatcgcta actccggtcg ctggttgcag
     3601 tggcactgga aaggtcagga cgcgccgggc gaagcgcgta acgacggcga aattctggcg
     3661 ggtatctacc atcatctgcg cgagctgtac caggccgaag gtggtaaagg cgtagaaccg
     3721 ctgatgaaga tgagctggaa ctacaagcag ccgcacgaac cgcaatctga cgaagtggct
     3781 aaagagaaca acggctacgc gctggaagat ctctatgacg ctaatggcgt gcttattgcg
     3841 aagaaaggtc agttgctgag tagctttgcg catctgcgtg atgacggtac aaccgcatct
     3901 tcttgctgga tctacaccgg tagctggaca gagcagggca accagatggc taaccgcgat
     3961 aactccgacc cgtccggtct ggggaatacg ctgggatggg cctgggcgtg gccgctcaac
     4021 cgtcgcgtgc tgtacaaccg tgcttcggcg gatatcaacg gtaaaccgtg ggatccgaaa
     4081 cggatgctga tccagtggaa cggcagcaag tggacgggta acgatattcc tgacttcggc
     4141 aatgccgcac cgggtacgcc aaccgggccg tttatcatgc agccggaagg gatgggacgc
     4201 ctgtttgcca tcaacaaaat ggcggaaggt ccgttcccgg aacactacga gccgattgaa
     4261 acgccgctgg gcactaaccc gctgcatccg aacgtggtgt ctaacccggt tgttcgtctg
     4321 tatgaacaag acgcgctgcg gatgggtaaa aaagagcagt tcccgtatgt gggtacgacc
     4381 tatcgtctga ccgagcactt ccacacctgg accaagcacg cattgctcaa cgcaattgct
     4441 cagccggaac agtttgtgga aatcagcgaa acgctggcgg cggcgaaagg cattaataat
     4501 ggcgagcgtg tcactgtctc cagcaagcgt ggctttatcc gcgcggtggc tgtggtaacg
     4561 cgtcgtctga aaccgctgaa tgtaaatggt cagcaggttg aaacggtggg tattccaatc
     4621 cactggggct ttgagggtgt cgcgcgtaaa ggttatatcg ctaacactct gacgccgaat
     4681 gtcggtgatg caaactcgca aacgccggaa tataaagcgt tcttagtcaa catcgagaag
     4741 gcgtaagggg gcgaacagat ggctatggaa acgcaggaca ttatcaaaag gtccgcaact
     4801 aactccatca cgccgccttc tcaggtgcgt gattacaaag cagaagtcgc aaaacttatc
     4861 gacgtttcca cctgtatcgg ctgtaaagcc tgtcaggtgg cgtgttcgga gtggaacgac
     4921 atccgtgatg aagtggggca ctgcgtcggg gtttacgata accccgccga tctgagcgcc
     4981 aagtcctgga cggtgatgcg ctttagcgaa accgaacaga acggcaagct ggagtggctg
     5041 atccgtaaag acggctgtat gcactgtgaa gatcccggct gcctgaaggc gtgcccgtct
     5101 gctggtgcaa tcattcagta cgctaacggg attgtcgatt tccagtcgga aaactgcatc
     5161 ggctgtggtt actgcattgc cgggtgtccg tttaatattc cgcgcctcaa caaagaggat
     5221 aaccgggtat ataaatgcac gctctgcgtc gatcgcgtca gcgtcggcca ggaaccggct
     5281 tgtgtgaaaa cctgtccgac cggggctatc cacttcggca ccaagaagga gatgctggag
     5341 ctggcggaac agcgcgtggc gaaactgaaa gcgcgtggtt acgaacatgc tggcgtctac
     5401 aacccggaag gggtcggtgg tacgcacgtt atgtacgtgc tgcatcacgc cgatcagccg
     5461 gagctgtatc acggtctgcc gaaagatccg aagatcgaca cctcggtaag cctgtggaaa
     5521 ggcgcgttga aaccgctggc agcggctggc tttattgcca cttttgccgg gttgattttc
     5581 cactacatcg gtattggccc gaataaggaa gtggacgatg acgaggagga tcatcatgag
     5641 taagtcgaaa atgattgtgc gcaccaaatt tattgatcgc gtctgtcact ggaccgtggt
     5701 gatttgcttc ttcctggtgg cgctgtccgg gatttcgttc ttcttcccga cgctgcaatg
     5761 gctgacgcaa accttcggta cgccgcagat gggacgcatt ttgcacccgt tcttcggcat
     5821 tgcgattttc gtcgcgctga tgtttatgtt tgtgcgtttt gtgcatcaca acatcccgga
     5881 taagaaagat attccgtggc tattgaacat tgtcgaagta ttaaaaggca atgagcacaa
     5941 agtggcggat gttggtaagt acaacgccgg gcaaaagatg atgttctggt cgatcatgag
     6001 catgattttc gtgctgttgg tgaccggggt gattatctgg cgtccgtact ttgcgcagta
     6061 cttcccgatg caggttgttc gctacagcct gctgatccac gcggctgccg gtatcatcct
     6121 gatccacgcc atcctgatcc atatgtatat ggcattctgg gtgaaaggat cgattaaagg
     6181 gatgatcgaa gggaaggtaa gccgtcgctg ggcgaagaaa caccatccgc gctggtatcg
     6241 tgaaatcgag aaggcagaag cgaaaaaaga gagtgaagaa gggatataat ctcttttgaa
     6301 ctttaagctg aaaatggcgc tttaagaagc gccatttttt tattgcgtaa ccagacggcg
     6361 cagtcgcgac acatccaccg ttttttcagc ctctgccaga ctccaggcgt tttgcagatt
     6421 cagccacatt tgcggcgaac tgccgatcac cacggaaagt ttaattgcca tttctggcgt
     6481 caaagctgct tttccggtca gcaatcggct tgccgttgag ggcgcaattt ccattgctct
     6541 ggcaaactcg cgcaggctga cattaagttc gtccagtgat tcctgaataa tgtcccccgg
     6601 gcggggatga ttggccattt tcattagtgt ctttactctc agtagcgttc cttctctggg
     6661 aacactatca ttattgttcc ttcctacgca acaataatcc atttagccat ttcatgttgg
     6721 agagtgtgcg atgctgcgac ccgaacatgg gagtcgcagc aaagacctct tagtgacgga
     6781 aatcaatcac catacggcca cggattttgc cttcttccat ctcggtaaag atggtgttga
     6841 tgtccgctaa cggacgcagg gcgactttcg gcaccacttt gccttcggcg gcaaactgga
     6901 aggcttcggt tagatcctgg cgcgtgccga ccagcgaacc gaccacttca atgccatcca
     6961 gcacaagacg tgggatatcc aggctcatag actccggcgg cagaccgaca gccacaacac
     7021 gaccgcctgc acggacagca tcaactgccg agttaaacgc agctttagct actgctgtta
     7081 ccaccgcagc gtgagcgcca ccggttttct cctgcacaat ttaggcggcg tcttcggtgc
     7141 gtgagttaat cgctaaatct gcacccattt cggttgccag ttttaactgc tcatcattga
     7201 catcaatggc gatcactttg gcgttaaaga cattcttcgc gtattgcagg gcgaggttac
     7261 ccagaccgcc aagaccgtag atagcaatcc actgaccggg acgaattttt gacagtttaa
     7321 cggctttgta ggtggtgacc cccgcacagg taatgctgct ggccgccgcc gagtccagac
     7381 catctggcac ttttaccgcg taatcggcga ccacgatgca ctcttccgcc atcccgccat
     7441 caacgctgta tccggcattt ttaactgaac ggcagagcgt ttcgttacca ctgttacagt
     7501 attcgcaatg accgcatcct tcgtaaaacc acgccacgct ggcacgatcg cctggtttta
     7561 atgaggtgac acctgggcca acttctgcca ccacaccgat gccttcatgg cccagaatta
     7621 ccccagtttt gtcaccaaaa tcgccattct taacatgaag atcggtatga catacaccac
     7681 aacactccat tttcagcagg gcttcgccat gtttcagtga gcgcagtgtt ttatccgtaa
     7741 cgtcaacatg atgatccttc gtaacaactg cagccttcat agttcctcct tttcggatga
     7801 tgttctgcat agcaggtgag gcaaatgaga tttattcgcc actacccagt atggatgaga
     7861 tctgaaaaag ggagagggaa atagcccggt agccttcact accgggcgca ggcttagatg
     7921 gaggtacggc ggtagtcgcg gtattcggct tgccagaaat tatcgtcaat ggcttgttgc
     7981 aaagcttcag cagacgtttt caccgccacg ccttgctgct gcgccatttt gccaaccgca
     8041 aacgcaattg cgcgggagac tttctgaata tctttcagtt ccggtagtac cagaccttcg
     8101 ccgttcagca ccagcggcga atactgagca agcgtttcac ttgctgacat cagcatctca
     8161 tcggtgatac gtgacgcgcc ggaagcaata acacccagcc cgatgccagg gaaaataaag
     8221 gcgttgttac actgggcgat agggtagatt ttatctttcc ataccactgg attaaacggg
     8281 ctgccagtgg cgaccagcgc gttaccttcg gtccaggcga taatgtcctg cggtgtggct
     8341 tccacgcgtg acgtcgggtt agacagcggc atcacgatcg gacgcggaca gtgtttatgc
     8401 atctcacgga tgatctcttc cgtaaacagc ccggtctgtc ctgagacgcc aatcagaata
     8461 tctggtttta cattgcgcac cacatccagc agtgacagca catcgctgtc ggtatcccag
     8521 tcactgaggt tttcgcgttt ctgcaccagt ttggtctgga aaggcagcag gttcggcatc
     8581 ttgtccgtca gcaggccaaa gcgatcgacc ataaagactt tctgccgcgc cgcttcctcg
     8641 cttaatcctt cgcgctgggt ctgggcgatg atcatttcgg caatgccgca tcccgctgaa
     8701 cctgcaccaa ggaagacgat ttttttctcg cttaactgac cacctgccgc tcggcttgct
     8761 gcgatcagtg tgccgactgt taccgccgca gtgccctgaa tgtcatcgtt aaaagaacaa
     8821 atttcattgc gatagcggtt aagtaacggc atcgcatttt tttgagcaaa gtcttcaaac
     8881 tgcaacagca cgtctggcca gcgttgtttc acagcctgga taaattcatc aacgaattca
     8941 tagtattcgt cgtcaatgat acgcggatta cgccagccca tatacagcgg atcgttaagc
     9001 agctgttggt tgttcgttcc gacatccagc accaccggaa gggtatacgc cgggctgatg
     9061 ccgccacagg cggtatagag cgacagttta ccgatcggaa tgcccatccc gccgatgccc
     9121 tggtcaccaa gccccagaat acgttcaccg tcagtcacca caatcacttt gatattatgg
     9181 ttcggtacgt tttgcagaat atcgtccata ttgtggcggt tctggtaaga gataaacacg
     9241 ccgcgtgaac ggcggtagat ctcagaaaaa cgctcacagg ctgcgccgac ggttggggta
     9301 taaataacag gcatcatctc atcaagatga ttgtttacca gacggtagaa gagggtttcg
     9361 ttggtgtcct ggatgttacg caggtagatg tgtttgtcga tttcggtttt gaatccctga
     9421 tactggatcc atgctcgttc cgcttgttct tcgatggttt cgaccacttc cggcagtaac
     9481 cccagcaggt tgaagttacg gcgttcttcc atgctgaagg cactgccttt attcaacaac
     9541 ggaaattcca gcagtacagg gccagcgtaa gggatataaa gcgaacgctg tttttttgtt
     9601 tttggttcca tgtcactcac tcttttttga atatccatcc ctggcacgct tttatcgtct
     9661 ttgctttacc gccagggcgt cggccccaag tataaagcag ataaaaacaa aaacaccatt
     9721 gcgcaggcaa tggtgtttaa tcgtcattga ggactgatgg ttatgaatta cttttcagcg
     9781 gggcgttttc tgccggttgg gttatttacc acgctggatt tgtcaccttc ggtaactatt
     9841 ttgcgctggt tagaaatttt atggtccagt ccaagaatat gacgtgcctg acggttcgat
     9901 ttcattttaa ctcctcaatc ctgttgctag ttttaaggac aacatcgccg tagcgaagaa
     9961 acacgtgcta aacccctaaa ttaggttgcc gatcaagcat agcaccttaa agcgtagggt
    10021 gctggccact gaccacataa ttgatcgttt gctggtagat atcactgagg atgtcgttat
    10081 cagaagcttc cacccatttg gtcagctcca tgagaatgtc atcttcagtg acaacaccgt
    10141 gctctgcccg aaggccttgc tcaatggcat taaccagagc gggttctgct gttgaatttt
    10201 ctgcctgata ataagtaaac atagtgattc tccgtgtctg tgtatttatg gtgtttgcta
    10261 cggatcgcag atttataaag cacattcagc atggcaaata tttgccgctt tgttgttaag
    10321 attagtcctt gttgatgatt tttatatttt aacctcatga tattgatagg tattattatt
    10381 gtggtgagtt gattaatatt aatgcaatat ctcttgggtt atatattccc ggttatctat
    10441 tgtggaaatt taatttaagt gcagaagtaa tatttctgcc ggatttttat tcggaatatc
    10501 ctgcttatcc tcgtgctgtt tctcacgtag tctataattt cctttttaag cccacatgag
    10561 agcaacaatg acaatccata agaaaggtca ggcacactgg gaaggcgata tcaaacgcgg
    10621 gaagggaaca gtatccaccg agagtggcgt gctgaaccaa cagccgtatg gatttaacac
    10681 gcgttttgaa ggcgaaaaag gaactaaccc tgaagaactg attggcgcag cgcatgccgc
    10741 atgtttctca atggcgcttt cattcatgct gggggaagcg ggatttacgc caacatcgat
    10801 tgataccatc gccgatgtgt cgctggataa agtggatgcc ggttttgcga ttacgaaaat
    10861 cgcactgaag agtgaagttg cggtgccggg tattgatgcc tctacctttg acggcatcat
    10921 ccagaaagca aaagcaggat gcccggtctc tcaggtactg aaagcggaaa ttacgctgga
    10981 ttaccagttg aaatcgtaaa gcattgccgg atgacgtgtc aggcgcgtga atgcctgatg
    11041 cgttgttagc atctctacag cgcccgccag caccggagct cacgtccgtc ctcccttatt
    11101 gctaatgatt gccggacttc acatccgctg gttgcaaggg ggcaacgttc gtagaaaaaa
    11161 cagccttgcg gcaacgtgcg gtttcccggc agatccgttt tacgtaatgc ccattcttcc
    11221 tccagcggtt tatcaatggc ggggagggaa tccagcaata atcgggtgta tggatgtgcc
    11281 ggagccgtta gcacctgttg cgcgtcgccc agttctacaa tctgcccgag atacatcacc
    11341 gccacccgat cgctcatatg acgtatcacc gagacattgt gtgaaatcag cacataggtc
    11401 agcccgtgat tttcctgtag cgttaccagt aaattgagga tctgcgcctg cacggagata
    11461 tccagcgcag aggttggctc atcaagcaca atcacgtcag gctgcgaaga gagtgctctg
    11521 gcaatggcga tacgttgccg ctgcccgccg gagaacgcat gaggcaggcg gtcgagatac
    11581 tccggacgaa tacccacctg cacagccagc tcctctacca acgctcgccg ctgttgttca
    11641 ctactacgct tagctatcca gagcggttct gtgatgatgc gccacaccgg taagcgcgga
    11701 ttgagcgaag agagcgggtc ctgaaacacc atctgcataa tgcgttgtga gccagaacgg
    11761 atgtactgcc cgtggctcgg ttgcagcata cccattaaaa gctgtgcgag ggtgcttttg
    11821 ccgcagcctg actcgccgac gatccctaag gtttcaccac ggcggatctg taaatcaata
    11881 ccattaatcg catgaacatg ttccgtagtt ttaccaagcc agtttttacg ggccgggaaa
    11941 ttgatatgga cgccgcgtaa cgttaataac gtgtcagaca cttatgacct cctgctgcgg
    12001 ataccaacag gcgcagcgct ggttgttgtc accacacgct gtcagcgccg ggacgttttc
    12061 acactgtgca cctgccgcat agcaacgatc gcgaaaagcg cagccgtcag gcaaatgggt
    12121 gaggtttggt accgtccctg gaatggcggg taatagctgg cgtggtactc catgttctgg
    12181 tgcacattgg agcaaaccaa tggtatacgg atgccgggga tgatggataa cgtctgccct
    12241 cacgccgctt tcaattacgc ttccggcata catcacgtaa acgctatcgc acagttgcga
    12301 caccacggcc atatcatggc tgatgaacag aaccgcagtt ccactggcgc gggctttatg
    12361 tttaagcaaa cgcagtacct gcaactgcac cgtgacgtcc agcgccgtag tcggttcgtc
    12421 ggcaataatc aattgcggct cgcaggagaa tgccagcgca atcattaccc gctggcgcat
    12481 accacctgaa agctcaaacg gatagcgcga cataacttcc acggcatccg ggatttgcat
    12541 ctcttccagc aggacaatcg ctttagctct ggcttcccga cgacttattg gttgatgatg
    12601 gcggatcacg tccatcatct gaagacctat tcgacgtgtc ggattgaggg cggtcatcgg
    12661 ttcctgaaag atcatcgcca ctcgtgcgcc gcgccactga cgaagctgtt tttcccgagc
    12721 gttaagaaca tcgtctccca gcagtgaaat ctgcccccga tgtacgcaat aactgcccgt
    12781 cggtagcaga cgcataatca gcattgcggt gactgattta cctgagccgg attctcccac
    12841 cagaccgaca atttcaccgc ggttaatctg caaggacaca ttgttgagcg cgtgaacatc
    12901 accgttaaaa ccggggaaac tcaaatgcag ctgttgaatg tccagaacgg gctgggtcat
    12961 gactgctttc ctcctgcttt cgggtcaagc agatcgcgaa taccatcacc aaagagatta
    13021 aacccaacgg cggtaagcaa aatcgctgcc cctggaaatg cgcaatacca ccactgatcg
    13081 agtacatagt tgcgaccatt cgctaccatc gcgccccatt cagcactcgg ttgttgagca
    13141 cccaggccaa taaatcccaa cgtggcggcc attaaaatcg cgctaccgat atccagcgat
    13201 gcctgcacga tcagcggcgg cagggagtta cgtaaaatat gccagctgat cagatgccaa
    13261 cgcgacgcac caaaggtttt cgccgcctga acataggtat actggcgtac aactaatgct
    13321 tgcccccgcg ccaggcgcac ataaaaggga attcgcacaa tagcaatcgc cagcatggcg
    13381 ttaaataaac tcggcccgag agcggcagcc agtgccattg tcagtaccaa cgaaggaatc
    13441 gacagcataa tgtccatgat gcgcatgatg atggcgtctg cgcgtccgcc aagcacaccg
    13501 gatagacatc cgagtagcga accaatcata cccgcaatgg caaccacgac taatccggcg
    13561 agaattgatt gctgactgcc gaccagtacg cggctgaaca gatcgcgtcc cacttcatcg
    13621 gtgccaaacc agtgcgccgc agaaggcggc aaaaggcggg cggttaaatc aatggcgttg
    13681 ggatcatgcg tcgcaagcca cggtgaaaaa aacatcatca atagcattaa tacaatgatg
    13741 actgcgccgg tcacggtgag cggactgcct ttcagcatcc atatcagttt tgcaccgtta
    13801 aatcgcgttt gtttttgtgg gcgtacggcg gacgtttcct cgcttagcat cattcaccac
    13861 ctccacgtcc gatacgcgga tcaatccaca aatagagcaa atccaccacc aggttgacca
    13921 gcacataagc aaatgaaacc acgacggcaa agcccatcac tgccgggaag tcgagcgcct
    13981 gtattgatgt tactacccat gcacccattc cgggccaggc aaaaacggtt tcggtgagca
    14041 ctgcgccata caacaaatcg cccagcgcca gacccaatac ggtaatcgat gggatcaacg
    14101 cattgggtag cgcataacat aaaacgatat accagccggg caagccgctg gcccgggcgg
    14161 tacgaatgta gtcttcactc aattgttcca gcatcgctga gcggatctgg cgagcgacaa
    14221 ttcccaggtg aacgaacgcc agcgttaatg ccggtaagat gagatgttgc aacgcattga
    14281 agaagacttc accgttgcct tcaagcagcg catcgagcag ataaaagccg gtaacgtgcg
    14341 ttggtggatc cagccagtca tcaagccttc cgccgccggg aagaatttgc agatgaccat
    14401 aaaacagcac aatgacgccc agtccaagcc aaaacgcagg tgtggagatt ccggtaatgg
    14461 ccattattcg caccagatga tccagccagc gatttcgcca gactgcagag agtatgccca
    14521 gcgggatgcc aatcaggagt gccagcagca gggcacaaaa agccagttcc agcgtcgccg
    14581 ggaaaaatat acgcaactct tccaacaccg gacgcccggt acgaatggat gttcccaggt
    14641 caccatgaaa caggtcgctg acgtagcggt aaaactgtac atacagtggc tggtccagtc
    14701 ccagttgctg gcgaatattt tccacgatag catcgctggc gcggtcaccc gccagtaacc
    14761 gcgccggatc gccagggatc agatgcgaga taataaacgt aatcacgcag acgcccgcca
    14821 tcacgagcac cagcccccag cagcgttggc gtaaaatact ccagaaggtc acgttattta
    14881 ctcatggtat tgatattgaa gacctgttcc agcatgggat tgaacacaaa gcctttcacc
    14941 tctttgttca tcg
//
LOCUS       DAJVTY010000099        14950 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVTY010000099 DAJVTY010000000
VERSION     DAJVTY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14950
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.099"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..443
                     /locus_tag="OQQ66_002673"
     CDS             <1..443
                     /locus_tag="OQQ66_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1905546.1"
                     /translation="NAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPA
                     KSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQ
                     MFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(484..1416)
                     /locus_tag="OQQ66_002674"
     CDS             complement(484..1416)
                     /locus_tag="OQQ66_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1905547.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1780..4566
                     /gene="polA"
                     /locus_tag="OQQ66_002675"
     CDS             1780..4566
                     /gene="polA"
                     /locus_tag="OQQ66_002675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1905548.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLQQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRLHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4948..5580)
                     /locus_tag="OQQ66_002676"
     CDS             complement(4948..5580)
                     /locus_tag="OQQ66_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1905549.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6162..6671
                     /gene="yihI"
                     /locus_tag="OQQ66_002677"
     CDS             6162..6671
                     /gene="yihI"
                     /locus_tag="OQQ66_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1905550.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6860..8233
                     /gene="hemN"
                     /locus_tag="OQQ66_002678"
     CDS             6860..8233
                     /gene="hemN"
                     /locus_tag="OQQ66_002678"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1905551.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8423..8533)
                     /locus_tag="OQQ66_002679"
     CDS             complement(8423..8533)
                     /locus_tag="OQQ66_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1905552.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8644..10053)
                     /gene="glnG"
                     /locus_tag="OQQ66_002680"
     CDS             complement(8644..10053)
                     /gene="glnG"
                     /locus_tag="OQQ66_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1905553.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10065..11114)
                     /gene="glnL"
                     /locus_tag="OQQ66_002681"
     CDS             complement(10065..11114)
                     /gene="glnL"
                     /locus_tag="OQQ66_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1905554.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11288..12697)
                     /gene="glnA"
                     /locus_tag="OQQ66_002682"
     CDS             complement(11288..12697)
                     /gene="glnA"
                     /locus_tag="OQQ66_002682"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1905555.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13070..14893
                     /gene="typA"
                     /locus_tag="OQQ66_002683"
     CDS             13070..14893
                     /gene="typA"
                     /locus_tag="OQQ66_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1905556.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3865 a         3750 c         3852 g         3483 t
ORIGIN      
        1 tgaatgctgc attgttcaaa gagtatttac cgttattaca acaaagtgag ccgaccatta
       61 aacaaccggt aagatggaag aacgcactcg gcgaactaaa tgccaatctg gatatcagta
      121 ttgccgaccc agccaaatct tcatcatcca caaacaaaga tatcaaatcg ctcaattttg
      181 atgtgaagtt accgcttaat gtcgtcacag aaaccgcaaa acagcttaat ttatctgaag
      241 gaatggatgc ggaaaaagcg caaaagcggg ctgataaaca aatcagcggg atgatgacct
      301 taggtcagat gtttcagtta atcacgattg acaacaatac cgcctcgctg caactgcgtt
      361 atacaccggg taaagttgtt tttaacggac aggagatgag cgaagaagaa tttatgtctc
      421 gtgccggacg ttttgttcat taaaatactc actcgcctct gctcattcag aggcgagcaa
      481 aatttacttg tcctgccgct gtgatgacat caacgacgtc agaagtcggt ctttttcctg
      541 ccacagtgag ttcagccatt gctgaaaatg gcgcttaaag cttttgtcat tgatgtagtc
      601 gccatgtaac tcatcggcaa taggctgtaa atccacatga acgacaattc gcgtcagttt
      661 accgcttaac atatcgaaga acggctgacg gttattgtcc ggataacaca gcgtaacatt
      721 aagcagttta tcgaattgtt tacccagtac atttagcgcc atcgcaatgc ctgccgcctt
      781 tggtggcaac aagttttgaa aagtggagtg ggtttgctga tgtttttctt gcgtgaaacg
      841 ggagccttca acgaaattga caatggtggt gggatgcagg cgaaactttt cacaagaacg
      901 ccgagtggtt tcaacatctt taccacgtcg ctccggatga cgtaataaat aagcgcggga
      961 ataacgcttc ataaacggca tatccagcgc ccagcacgcc aggccaagaa atggcaccca
     1021 ggccagctgt tgcttgagga aatatttatt catcggaatg tgcttacgaa acagcacgca
     1081 cagtacgaca atatctgccc aactacggtg gttacaaata agcagatacc agttcttctt
     1141 acttaaccct tccagcccgt gaacttccca ttgcaggtgt gggttcagat gcagtaatac
     1201 cgccagacct tcacaccagc aatacatcat aaaatcacaa aaacgcgaaa cctttcgcca
     1261 aatgactggc acaggtagca atagttttac aatcccggca atgatgatcg ggacggaaca
     1321 aaaaatagtg accaaaatgg tcaatacgat actcagcaaa agggttatcg cagcgagtat
     1381 tctcgtcata atgaatttat tcaaaaggtt agccatagac agtgcgctaa agaagcgcaa
     1441 ggcgcagatt ttagcagaaa acgacccaaa taacggatga tccttaagga gaaaaataat
     1501 tcatatctat ccacattaga aaaaatccca ttatctcaat tattagggat ggctttattt
     1561 ttaactacat gaaaaacaaa gacaaacatc atactgtaaa aagcatgata ataaattaaa
     1621 agcgatgtaa ataatttatg cacaaagtta tccacatgac gatttgcgag cgatccagaa
     1681 gatctacaaa agattttcac gaaaagcggt gaaaaactca tgtttttatc ctgtctgtgg
     1741 catcctttac ccataatctg ataaacaggc acggacatta tggttcagat cccccaaaat
     1801 ccacttatcc ttgtagatgg ttcatcttat ctttatcgcg catatcacgc gtttcccccg
     1861 ctgactaaca gcgcaggcga gccgaccggt gcgatgtatg gtgtcctcaa catgctgcgc
     1921 agtctgatca tgcaatataa accgacgcat gcagcggtgg tctttgacgc caagggaaaa
     1981 acctttcgtg atgaactgtt tgaacattac aaatcacatc gcccgccaat gccggacgat
     2041 ctgcgagcac aaatcgaacc tctgcacgcg atggttaaag cgatgggact gccgctgctg
     2101 gcggtttctg gcgtagaagc ggacgacgtt atcggtactc tggcgcgcga agccgaaaaa
     2161 gccgggcgtc cggtgctgat cagcactggc gataaagata tggcgcagct ggtgactcca
     2221 aatattacgc ttatcaatac catgacgaat accatcctcg gaccggaaga ggtggtgaat
     2281 aagtacggcg tgccgccaga actgatcatc gatttcctgg cgctgatggg tgactcctcc
     2341 gataacatcc ctggcgtacc gggcgtcggt gaaaaaaccg cgcaggcatt gctgcaaggt
     2401 ctgggcgggc tggatacgct gtatgccgag ccagaaaaaa ttgctgggtt gagcttccgt
     2461 ggcgcgaaaa caatggcagc gaagctccag caaaacaaag aagttgctta tctctcatac
     2521 cagctggcga cgattaaaac cgacgttgaa ctggagctga cctgtgaaca actggaagtg
     2581 cagcaaccgg cagcggaaga gttgttgggg ctgttcaaaa agtatgagtt caaacgctgg
     2641 actgctgatg tcgaagcggg caaatggtta caggccaaag gggcaaaacc agccgcgaag
     2701 ccgcaggaaa ccagtgttgc agacgaagcg ccagaagtga cggcaacggt gatttcttat
     2761 gacaactacg tcaccatcct tgatgaagaa acactgaaag cgtggattgc gaagctggaa
     2821 aaagcgccgg tatttgcatt tgataccgaa accgacagcc ttgataacat ctctgctaac
     2881 ctggtcgggc tttcttttgc tatcgagcca ggcgtagcgg catatattcc ggttgctcat
     2941 gattatcttg atgcgcccga tcaaatctct cgcgagcgtg cactcgagtt gctaaaaccg
     3001 ctgctggaag atgaaaaggc gctgaaggtc gggcaaaacc tgaaatacga tcgcggtatt
     3061 ctggcgaatt acggcattga gctgcgtggg attgcgtttg ataccatgct ggagtcctac
     3121 attctcaata gcgttgccgg gcgtcacgat atggacagcc tcgcggaacg ttggttgaag
     3181 cacaaaacca tcacttttga agagattgcg ggtaaaggca aaaatccact gacctttaac
     3241 cagattgccc tcgaagaggc cggacgttac gccgccgaag atgctgatgt caccttgcag
     3301 ttgcatctga aaatgtggcc ggacctgcaa aaacacaaag ggccgttgaa cgtcttcgag
     3361 aatatcgaaa tgccgctggt gccggtgctt tcacgcattg aacgtaacgg tgtgaagatc
     3421 gatccgaaag tgctgcacaa tcattctgaa gagctcacct tgcgtctggc tgagctggaa
     3481 aagaaagcgc atgaaattgc aggtgaagaa tttaaccttt cttccaccaa gcagttgcaa
     3541 accattctgt ttgaaaaaca gggtattaaa ccgctgaaga aaacgccggg tggcgcgccg
     3601 tcaacgtcgg aagaggtact ggaagaactg gcgctggact atccgttgcc aaaagtgatt
     3661 ctggagtatc gtggtctggc gaagctgaaa tcgacctaca ccgacaagct gccgctgatg
     3721 atcaacccga aaaccgggcg tttgcatacc tcttatcacc aggcagtaac tgcaacggga
     3781 cgtttatcgt caaccgatcc taacctgcaa aacattccgg tgcgtaacga agaaggtcgt
     3841 cgtatccgcc aggcgtttat tgcgccagag gattatgtga ttgtctcagc ggactactcg
     3901 cagattgaac tgcgcattat ggcgcatctt tcgcgtgaca aaggcttgct gaccgcattc
     3961 gcggaaggaa aagatatcca ccgggcaacg gcggcagaag tgtttggttt gccactggaa
     4021 acagtcacca gcgagcaacg ccgtagcgcg aaagcgatca actttggtct gatttatggc
     4081 atgagtgctt tcggtctggc gcggcaattg aacattccac gtaaagaagc gcagaagtac
     4141 atggaccttt acttcgaacg ctaccctggc gtgctggagt atatggaacg cacccgtgct
     4201 caggcgaaag agcagggcta cgttgaaacg ctggacggac gccgtctgta tctgccggat
     4261 atcaaatcca gcaatggtgc tcgtcgtgca gcggctgaac gtgcagccat taacgcgcca
     4321 atgcagggaa ccgccgccga cattatcaaa cgggcgatga ttgccgttga tgcgtggtta
     4381 caggctgagc aaccgcgtgt acgtatgatc atgcaggtac acgatgaact ggtatttgaa
     4441 gttcataaag atgatgttga tgccgtcgcg aagcagattc atcaactgat ggaaaactgt
     4501 acccgtctgg atgtgccgtt gctggtggaa gtggggagtg gcgaaaactg ggatcaggcg
     4561 cactaagatt cgcctgaaca tgcccttttt tcgtaagtaa gcaacataag ctgtcacgtt
     4621 ttgtgatggc tattagaaat tcctatgcaa caactgaaaa aaaattacaa aaagtgcttt
     4681 ctgaactgaa caaaaaagag taaagttagt cgcgtagggt acagaggtaa gatgttctat
     4741 ctttcagacc ttttacttca cgtaatcgga tttggctgaa tattttagcc gccccagtca
     4801 gtaatgactg gggcgttttt tattgggcga aagaaaagat ccgtaatgcc tgatgcgcta
     4861 tgtttatcag gccaacggta gaattgtaat ctattgaatt tacgggccgg atacgccaca
     4921 tccggcacaa gcattaaggc aagaaaatta ttcgccgtcc tgcgtttctt ctacaggctg
     4981 catctcgcta aaccaggtat ccagtttctg ccgcagcttg tccacgcctt gtttcttcaa
     5041 cgaagaaaac gtttcaacct gcacatcacc gttaaacgcc agtacagctt cacgcaccat
     5101 attcaattgc gctttacgtg cgccgcttgc cagtttgtcc gctttggtca gcagcaccag
     5161 aacggcgata ttgctgtcta ccgcccactc aatcatctgc tgatccaaat ctttcagcgg
     5221 atggcgaata tccattagca ccaccagacc ttgcaggcta tgacgttttt cgaggtattc
     5281 gccgagcgca cgctgccatt tgcgcttcat ctcttccggg acttccgcat aaccgtaccc
     5341 aggcaagtca accagacgct tgccgtcagc cacttcaaac aggttgataa gctgggtgcg
     5401 ccctggggtt tttgaggtac gagccaggct tttctggtta gtcagcgtgt tcagcgcgct
     5461 ggatttacct gcgttggaac ggcctgcaaa agccacttca attccggtat cggaaggtag
     5521 gtggcgaata tcaggcgcac tcatcacaaa atgcgtctgt tgataattca aattagtcaa
     5581 agcggtcgtc tccgtcagtc aaagctttgt ggcgattata cctgaaccat aataaaaggc
     5641 ggatttttcg gcgtgagcgt tgtaagtaaa agccatacgc tttgtgagac attgccgata
     5701 gtctttatgc gaaatagcag agaaaattct gcgatgcatg tcaaataagc tatataaatc
     5761 agtgaattgc ttttatataa aactctgaaa atgtgcaaat actgatggcg gttgattgtt
     5821 tgtttaaagc gaaggcgtaa agtagcaccc atagagcgag gacgctaaca ggaacaatga
     5881 ctcaggatga gggtcaggag cgccaggagg cgaagacaga ggattgtcag gaagacaaac
     5941 gtccggagac gtaattaaac ggaaatggaa tcaacacgga ttgttccggc taaaggaaaa
     6001 acagggtgtg ttggcggcct gcaaggattg taagacccgt taagggttat gagtcaggaa
     6061 aaaaggcgac agagtaatct gtcgcctttt ttctttgctt gctttctgtt agattccgcc
     6121 gcaaatcgat actgaataaa acggctaaaa gacgaaccat tatgaaacca tcatcttcaa
     6181 actcacgcag caaaggtcat gcaaaagcgc gtcgaaaaac acgcgaggag ctggatcagg
     6241 aagctcgtga tcgcaagcgt cagaaaaaac gtcgtggtca tgcgccgggc agccgcgcag
     6301 cgggcggaaa caccacgtca ggcagcaaag gccagaacgc accaaaagat ccacgtattg
     6361 gcagtaaaac ccctattcca ttgggcgtga ctgaaaaagt caccaaacag cacaaaccga
     6421 agagtgagaa acctatgctt tcaccgcagg cggagttgga attactggaa acggatgagc
     6481 gtctggatgc gctgctggaa cgtctggaag caggcgaaac cctgagtgcc gaagagcaat
     6541 cctgggtgga tgctaaactg gatcgtattg atgagttgat gcagaaactc ggcctctctt
     6601 atgacgatga cgaagaagag gaagaagacg agaagcaaga agacatgatg cgtctgctgc
     6661 ggggcaacta acggattgcc gccgtgggcc ttcccgttct gctgataacc cttccggtta
     6721 tatgttatct ggtgtggtta ttcgttaaac tacagcggtt gtcgcggcga caaaagtggc
     6781 tgcgcaaccg gctgatgacc cgaaacgggc atccgccggt acgccgtagc cgccagagac
     6841 gccatcggaa ggagtgagca tgtctgtaca gcaaatcgac tgggatctgg ccctgatcca
     6901 gaaatataac tattccgggc cacgatacac ctcgtacccg accgcgctgg agttttcaga
     6961 agacttcggc gaacaggcgt ttttacaagc cgtggcgcgc tatcctgagc gtccattatc
     7021 tctctacgta catatcccgt tctgccataa gctttgttac ttctgcggtt gcaataagat
     7081 tgttactcgc cagcagcaca aggccgatca gtatctggac gcgctggagc aagaaatcgt
     7141 ccatcgtgca ccgctgttcg ccgggcgtca cgtcagccaa ttgcactggg gcggcggaac
     7201 gccgacgtat ctgaataaag cgcaaatcag ccgcctgatg aagctgctgc gcgaaaactt
     7261 ccagttcaat gccgatgcgg agatttcgat cgaagtcgat ccgcgggaaa tcgaactgga
     7321 tgtactcgat catttacgcg ccgaaggctt taatcgcctg agcatgggcg tgcaggactt
     7381 caacaaagaa gtgcaacgtc tggttaaccg cgagcaggat gaagagttca tctttgcact
     7441 gcttaaccat gcgcgtgaga ttggttttac ctccaccaac atcgacctga tttacggcct
     7501 gccgaaacag acgccggaga gtttcgcctt taccctgaaa cgtgtggcgg agctgaaccc
     7561 cgatcgtctg agtgtcttta actacgcgca tctgccgacc atttttgctg ctcagcgcaa
     7621 aatcaaagat gctgacctgc cgagtccgca gcaaaaactc gatatcctgc aggaaaccat
     7681 cgccttcctg acgcaatcgg gctatcagtt tatcggtatg gatcactttg cccgtccgga
     7741 tgacgagctg gcggtggccc agcgtgaagg cgtgctgcat cgtaacttcc agggctacac
     7801 cactcagggc gataccgatc tgctggggat gggcgtttcc gccatcagca tgattggcga
     7861 ctgctacgcg cagaaccaga aagagttgaa gcagtactat cagcaagtgg atgaacaagg
     7921 caatgcgctg tggcgtggta ttgcgctaac gcgtgatgac tgtattcgcc gcgatgtgat
     7981 taagtcgctc atctgcaact tccgtctgga ttacgcccct attgagaaac agtgggattt
     8041 gcacttcgct gattactttg cggaagatct caagctgctc gccccgttag caaaagatgg
     8101 gctggtggat gtggatgaga agggaataca ggtgacggcg aaaggtcgct tgctgatccg
     8161 caacatttgc atgtgctttg atacctatct gcgccagaaa gcgcggatgc agcagttctc
     8221 acgggtgatt tgaatagtga atggcgcttc gtttacaaag tagaaaacga aagcgccatc
     8281 aatgtgctga gaacaagatt gcctgatgcg ctacgcttat caggcctaca aaacctgttg
     8341 aaaatattgc ttttgtaggc cggataaggc gttcacgccg catccggcat gaacaaagag
     8401 cacttcatca gcaatctgaa actcaactaa acagcccaat cattgcggca cacagcacgg
     8461 cagcaacggc agtttgcggt gtgtggctgt caactgcgcc actcgatacc agattaatta
     8521 ttgattccag cataatgact ctccccgttt tccgggcaga tcatactgaa cttatcggaa
     8581 cagtaaagcg taaaatacca gcaatttgcg ctcaataatc aatctttaca cacaagctgt
     8641 gaatcactcc atccccagct cttttaactt acgcgtcagg gtgttgcggc cccagccgag
     8701 tagccgcgcc gcttcctgtt tatgcccctg cgtatgtcgc aacgcggtcg tcagtaacgt
     8761 ccgctccagc tctggctgcg cttcggaaag cagattttga tgaccggaac gcagcgctct
     8821 gtctgcccac tgcgcaagaa gcgtcgccca gctgtccggt tgcatttgcg aagtactctc
     8881 cgcaaccgtt gattcaaaca gttcgccggg caaatcctga atcaacactt cctgcccggc
     8941 ggccatcacc gttagccagc ggcaggtgtt ttccagctgg cgcacgttgc ctggccacgc
     9001 cagacgcgtc agagcagctt cggtttccgg atgcagtaac ttcgcttcta cgcccagttc
     9061 gcgcgcggca acctgtaaaa aatggcgcgc cagacgggga atatcttccc gacgttcgcg
     9121 cagcggcggt agatgaacgc ggataacgtt taggcggtgg aacagatcct cacggaactt
     9181 accttcctgc actcgctgtt cgagattctg gtgagtggca gcgataatcc gcacatccac
     9241 tttcaccggc gcatagccgc caacgcggta aaactgaccg tctgccagca cgcgcagcaa
     9301 acgcgtctgc acatccagcg gcatatcacc aatttcgtcg aggaataatg taccgccatc
     9361 ggcctgttca aaacgcccct gacgaatggt attcgcgcca gtaaacgcgc ctttctcgtg
     9421 gccaaacagt tctgattcga tcaaatcttt tgggatagct gccatattca gcgcgataaa
     9481 cggtgctttg gcgcgcggac tgtggcgatg cagggcatga gcgaccagtt ctttaccggt
     9541 gccggattcg ccgttaatca gcacgctaat agaagaacgc gaaagccgac cgataatacg
     9601 gaacacgtcc tgcatggctg gcgcttcgcc gatgatatcg gtcgttgggc cgttaagctg
     9661 aatattacgc ggctgctgct gttcctggta atgactgata gcgcgctcaa ccagcgccac
     9721 ggcttcgtcg atatcaaacg gtttgggcag ataatcaaac gccccttgtt gataggcgct
     9781 gacggcagca tccagatcgg aatgtgcggt cataatgatg accggaagca ttggatggcg
     9841 ctgtttaatc tgcttgagca gcgccagccc gtccattccc ggcatacgga tatctgaaag
     9901 cagcacatcc ggcgttttgc tcgccagcgc ctccagtact tccgcgccgt tctcaaatgt
     9961 cgtacaggtt aaacccgctc cagcgagcgc acgttcaagc acccaacgga tggaactatc
    10021 gtcatcgact acccagacta tccctcgttg cataaacgtc acctttattt cctgataggc
    10081 aggtaaaccg agaactcggt atgacctggc caactggtaa attcaatttt gcctgaatgc
    10141 tgatcaatca aattacgggc gatggataag ccaagcccgg tgccaccttc gcggccgctg
    10201 accatcgggt aaaacagcgt atcctgcaaa tgaggcggaa tgcccggccc gttatcttcc
    10261 acatcaatcc gcgccgccag ccggtagcgc tcgccgtgta aggtcagttg aaacgcggtg
    10321 cgggtacgca gaatgatttc accgccttcc ggccccagcg cctgtagcgc attgcgcaca
    10381 atattcagca agacctgttc aatttgatcc gggtcgtgcg ccagttccgg caggctggga
    10441 tcgtaatcac gaatcaaccg cacgttgtcc ggcagttcca tcgacaccag cgttaccacg
    10501 cgttcagcca ctttgtgaat actttcggta acgcgcgtac cgggcagctg cggccccaac
    10561 agacggtcga ccagatttcg cagccggtcc gcctgttcga taatcacttt ggtatattcg
    10621 agtagtgatg ggtcaggtaa cgctttgctg agcagctgcg ccgcgccacg taaaccgcca
    10681 agcggatttt taatctcatg tgccaggccg cgcactaaat cacgggcagc aacctgctgg
    10741 gcgtgctgta gctgttcctg acttaagcgg cgctggttat ccatcggagc catctccagc
    10801 aggatcatgc cgtccggcat acgctgggcc gtcacagaaa ggatatgcga gcgcccgtcg
    10861 atgaccagcg tcacttcgtt atcggtaaaa ccttgccccg cctccagact ttcttgcatc
    10921 agctcgatat ttaatgagaa gtagctcaac agttccggta acggtgtacc aaacaatttg
    10981 cgggagcttt gggcgagcag ttgttgcgcg gcagggttgg cgtaatggat ctccaggttg
    11041 tcatcgatta acaaaatact gttaatcagc gagttgagga tctgcccagc atcgggctgc
    11101 gtgcctgttg ccataaagca gtctcctgaa caggttgcac cattttagtg cattatagct
    11161 ttttacggat aaaaagcgcg aagcatcaga gaattgacgg agaaaaaagc ccatgcagag
    11221 atgggctgaa agtttccacg gcaactaaat cccggcgttg ttgcgccggg tagtacaact
    11281 tcaactatta gacgctgtag tacagctcaa actctaccgg atgcggagtc atacgcacgc
    11341 ggtcatcttc ttcgcgacgc agagcgatgt acgcatcaat tgcttcgtca gtgaacacgc
    11401 caccggcttt caggaactcg cggtccagat ccagttcgtt cagtgcttct tccagagagc
    11461 ctgcaacctg tgggatctct ttcgcttctt ctggcggcag gtcatacagg tttttgtcca
    11521 tggcttcacc cggatggatc ttgttcttga taccatcaag accggccatc agcagggcag
    11581 caaagcacag gtacgggtta gctgccggat ccgggaaacg tacttcgata cgacgtgctt
    11641 tcggagaaga aaccaccgga atacggatag acgcagaacg gttacgcgca gagtaagcca
    11701 gcattaccgg tgcttcatag cccgggacca gacgcttata agagttggtg gtcgggtttg
    11761 ccagggcgtt aatcgcttta gcgtgtttga ttacgccgcc aatgtagtac agcgcctgct
    11821 cagacagacc tgcgtatttg tcgcctgcga acaggttaac gccgttttta gacagagaca
    11881 tgtggcagtg cataccggag ccgttatcac cgaacatcgg ttttggcata aaggtcgcgg
    11941 ttttaccgaa gcggtgcgct acgttgtgca caacatattt gtagatctga atttcgtcag
    12001 cttttttggt catggtattg aagcgggtag ccacttcgtt ctgaccagca gtcgctactt
    12061 cgtggtgatg ggcttcaacc accagcccca tctgttccat caccagacac atttcagaac
    12121 gaatatcctg agccgagtct accggtggaa ccgggaagta accgcctttc actgccggac
    12181 ggtgaccttt gttaccacct tcgtattggg tggaggagtt ccatgcgcct tcgatatcgt
    12241 cgatagcaac gtgggaaccg gagatagatg atccgaaacg gatgtcatcg aacaggaaga
    12301 attcaggttc tggcccgaac agtactgtgt cggcaatgcc agtggaacgc aggtaatctt
    12361 cggcgcgctt cgcaatggag cgcgggtcac ggtcatagcc ttgcagggtg ccaggttcaa
    12421 ggatgtcgca acggataatc agggtggagt cggcgaagaa cgggtcaatc actgcggtgg
    12481 atgcgtctgg catcagcacc atgtcggact cgttaatgcc tttccagccg ccaatcgagg
    12541 agccgtcaaa cattttgcct tcttcgaaga attcagcatt cacctgatga gcagggatag
    12601 tgacgtgctg ttctttacct ttagtatcgg tgaagcgcaa atcaacaaac ttcacttcgt
    12661 gctcgttcag catcgtcagt acgtgttcag cggacatact ttaactctcc tggattggtc
    12721 atggtcgtcg tggtaacgaa atctgcaatt attttggccg tgtcgccgta aaaaagataa
    12781 agcgaaatct gtgccaactt ttaaattgcc cctaaaaggc gttatcatgc gcaccatcgt
    12841 gcaaaagggc tgcaccacga tgtgaatgtt gcaccaatat agtgcttcaa tggaaacatt
    12901 aagcaccatg ttggtgcaat gacctttgga taaccctttt tatgctccgt gaaagcgatc
    12961 acaaagggac tctgcaatac ttgtttgcgg aggatgtttg tgatcctgtt ttgtagtgcg
    13021 attaatccgt gtacaataac gcgctatttc taatgcctga ggcaaagttg tgatcgaaaa
    13081 attgcgtaat atcgccatca tcgcgcacgt agaccatggt aaaaccaccc tggtagacaa
    13141 gctgctccaa caatccggta cgttcgactc tcgtgccgaa acccaagagc gcgtgatgga
    13201 ctccaacgat ttggagaaag agcgtgggat taccatcctc gcgaaaaaca ccgctatcaa
    13261 atggaatgat taccgtatca acatcgttga taccccgggg cacgccgact tcggtggtga
    13321 agttgaacgt gtaatgtcca tggtagactc agtgctgctg gtggttgacg catttgacgg
    13381 cccgatgccg caaacgcgct tcgtaaccaa aaaagcgttt gcttacggcc tgaagccgat
    13441 tgttgttatc aacaaagttg accgccctgg cgcgcgtcct gattgggttg tggatcaggt
    13501 attcgatctg ttcgttaacc tcgacgcgac cgacgagcag ctggacttcc cgatcgttta
    13561 cgcttctgcg ctgaacggta tcgcgggtct ggaccacgaa gatatggcgg aagacatgac
    13621 cccgctgtac caggcgattg ttgaccacgt tcctgcgccg gacgttgacc ttgacggtcc
    13681 gttccagatg cagatttctc agctcgatta caacagctat gttggcgtta tcggcattgg
    13741 ccgcatcaag cgcggtaaag tgaagccgaa ccagcaggtc actatcatcg atagcgaagg
    13801 caaaacccgc aacgcgaaag tcggtaaagt gctgggccac ctcggtctgg aacgtatcga
    13861 aaccgacctg gcggaagctg gcgatatcgt tgcgatcacg ggccttggcg aactgaacat
    13921 ttctgacacc gtttgcgaca cgcaaaacgt tgaagcgctg ccggcactct ccgttgatga
    13981 gccgaccgtt tctatgttct tctgcgttaa cacctcgccg ttctgcggta aagaaggtaa
    14041 gttcgtaacg tctcgtcaga tcctggatcg tctgaacaaa gaactggtac acaacgttgc
    14101 gctgcgcgta gaagaaaccg aagacgctga tgcgttccgc gtttctggtc gtggcgaact
    14161 acacctgtct gttctgatcg aaaacatgcg tcgtgaaggt ttcgaactgg cggtatcccg
    14221 tccgaaagtt atcttccgtg aaatcgacgg tcgtaaacaa gagccatatg aaaacgtgac
    14281 tctggacgtt gaagaacagc atcagggttc tgtaatgcag gcgctgggcg aacgtaaagg
    14341 cgacctgaaa aacatgaatc cagacggtaa aggccgcgta cgtctcgact acgtgatccc
    14401 aagccgtggt ctgattggct tccgttctga gttcatgacc atgacttccg gtactggtct
    14461 gctgtactcc accttcagcc actacgacga cgtacgtccg ggtgaagtgg gtcagcgtca
    14521 gaacggcgta ctgatctcta acggtcaggg taaagcggtc gcgttcgcgc tgttcggtct
    14581 gcaggatcgc ggtaagctgt tcctcggtca cggtgcagaa gtttacgaag gtcagattat
    14641 cggtattcat agccgctcta acgacctgac tgtaaactgc ctgaccggta agaaactgac
    14701 caacatgcgt gcttccggta ctgacgaagc cgttgttctg gttccgccta tccgcatgac
    14761 tctggaacaa gctctggagt tcatcgatga tgacgaactg gtagaagtga ctccgacctc
    14821 tatccgtatt cgtaaacgtc acctgacgga aaacgatcgt cgtcgcgcca accgcgcacc
    14881 gaaagactat taatttcgtt ttttagttat aaaaaacctg ccatcgctgg ctaatgccag
    14941 tcagttaagc
//
LOCUS       DAJVTY010000100        14877 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVTY010000100 DAJVTY010000000
VERSION     DAJVTY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.100"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..261)
                     /locus_tag="OQQ66_002684"
     CDS             complement(<1..261)
                     /locus_tag="OQQ66_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCS1905557.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGCIKRQMFTKLRTTKYLKTAASA"
     gene            complement(254..685)
                     /locus_tag="OQQ66_002685"
     CDS             complement(254..685)
                     /locus_tag="OQQ66_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1905558.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            complement(781..1209)
                     /locus_tag="OQQ66_002686"
     CDS             complement(781..1209)
                     /locus_tag="OQQ66_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCS1905559.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(1206..1583)
                     /locus_tag="OQQ66_002687"
     CDS             complement(1206..1583)
                     /locus_tag="OQQ66_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905560.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITHNQMAQQVAA"
     gene            complement(1585..2097)
                     /locus_tag="OQQ66_002688"
     CDS             complement(1585..2097)
                     /locus_tag="OQQ66_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1905561.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALEKCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(2078..2293)
                     /locus_tag="OQQ66_002689"
     CDS             complement(2078..2293)
                     /locus_tag="OQQ66_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905562.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2297..2500)
                     /locus_tag="OQQ66_002690"
     CDS             complement(2297..2500)
                     /locus_tag="OQQ66_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCS1905563.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2500..2964)
                     /locus_tag="OQQ66_002691"
     CDS             complement(2500..2964)
                     /locus_tag="OQQ66_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCS1905564.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERFQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(3060..3710)
                     /locus_tag="OQQ66_002692"
     CDS             complement(3060..3710)
                     /locus_tag="OQQ66_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCS1905565.1"
                     /translation="MSLSPARQHRLRVQAEQAVREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3714..4772)
                     /locus_tag="OQQ66_002693"
     CDS             complement(3714..4772)
                     /locus_tag="OQQ66_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCS1905566.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            complement(4789..5622)
                     /locus_tag="OQQ66_002694"
     CDS             complement(4789..5622)
                     /locus_tag="OQQ66_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCS1905567.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTFMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5765..7531
                     /locus_tag="OQQ66_002695"
     CDS             5765..7531
                     /locus_tag="OQQ66_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCS1905568.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7531..8127
                     /locus_tag="OQQ66_002696"
     CDS             7531..8127
                     /locus_tag="OQQ66_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905569.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            8161..8562
                     /locus_tag="OQQ66_002697"
     CDS             8161..8562
                     /locus_tag="OQQ66_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1905570.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(8669..9550)
                     /locus_tag="OQQ66_002698"
     CDS             complement(8669..9550)
                     /locus_tag="OQQ66_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905571.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(9534..11183)
                     /locus_tag="OQQ66_002699"
     CDS             complement(9534..11183)
                     /locus_tag="OQQ66_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1905572.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(11476..11709)
                     /locus_tag="OQQ66_002700"
     CDS             complement(11476..11709)
                     /locus_tag="OQQ66_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCS1905573.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(11720..11908)
                     /locus_tag="OQQ66_002701"
     CDS             complement(11720..11908)
                     /locus_tag="OQQ66_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905574.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(12062..14476)
                     /locus_tag="OQQ66_002702"
     CDS             complement(12062..14476)
                     /locus_tag="OQQ66_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCS1905575.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(14473..>14877)
                     /locus_tag="OQQ66_002703"
     CDS             complement(14473..>14877)
                     /locus_tag="OQQ66_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HCS1905576.1"
                     /translation="KAQRATFICASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFT
                     EDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKS
                     ATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         3602 a         4049 c         3446 g         3780 t
ORIGIN      
        1 ggcgctggct gcagttttca ggtattttgt ggtgcgcagc tttgtaaaca tctgacgttt
       61 gatgcacccc tttttgctgc gtgctgttac cctgcgcggc tcataactgc tgccatcagg
      121 attgcgctgc atcctgatgt tctgctgctg tgtccggcgc agttcctgcg ccagctggcg
      181 catcatgcgg cttcttgcgg ctggctccag attcgccaat aatgcactca gccagtcgtc
      241 caccttctgc aattcagcca cgtttcaccg tccacatttc ttcaggttca tcaggttccg
      301 ctaccgcttc aacgttcgac acactgccgt cagtgctgac cagcacacgc tccgtcagtt
      361 gcaggttaag gctgatatca cagacatcgt tgcgcagaat atccacctca aaggtgaata
      421 gcttttcccg taacgccggg ttattgatcg catcgggctg gttatcccgc agccacagca
      481 aaaccggggc catcagcaga ttctggtcgc cgctgaaatc ttcaatcacc acgttcaggg
      541 tgtagcggta ctcccatgac atggagctag ccccggaggc aaccagcgaa ccgttatcca
      601 caaacagatg cagtttgtcc gggttattac ggacataagg cactgcttta ttgagggcgt
      661 ggcgcaggga ttgtggtttg ttcactgttt cgctcctgac acgcaataat catgtccact
      721 ttgtctgcac agaccgccca ggcggcctcc gtttcatcca gcaacgcatt cagatcaccg
      781 ttagtgcgcg gcgctgcctg ctccagccga cacggcgtca ctcgcggaca accactgacg
      841 gtaagctgca cctccggtga gtgccggacg ttcccgcagc cggataatgt cagcaggcaa
      901 aggagtatca gcccagcgac gtaaatcctc gttctcacgt ttcagttcct caatccggcg
      961 ttgtcgttgt ctcagcagta cactggtctg ttctgcttcg gcatagagcc gcgcctgctc
     1021 ccggttattg gtttcagtca gaatggacag gctgataagc tggctgttgc tctttgccag
     1081 tgcctggctt ttgctctgca gctcgtctgc ctgcgtgctg atggtctggc tggcatcagc
     1141 cagccgccac gtctgccagc ccagcgccgc caataataac gccagcacaa ccagcagcaa
     1201 ccggttcatg cggctacctg ttgcgccatc tgattatggg tgatccagaa ggcaataacg
     1261 gtcagcagat aaaagaccag ggtaatggcc caccccgtcc aggcgagact gacgacaatc
     1321 agcaatcgta tcacccagct gataaatacg ttttcttttc gggtaattgt cttcagtaaa
     1381 gatgccctca actcctgcca gagcgggcca ttcttaatta acgaagccag tgctaccgga
     1441 attaccgccc atgtcagtaa acaggctacc caaacgccgg acgctgccag taccggaaaa
     1501 atcccctgcg gatacaccat tgctgcgatt aacagcgcca tccataacat cagaaacagc
     1561 ccgctgatta atttcttttt catttcagtt tgctccctgt aaacaccagg ccatctcccg
     1621 cgcacggcgg ttatccagcc cctgattaaa aacacctttc acataaaccc agcgcggcaa
     1681 ctgtcggcac gcatccgccc agcgccgctg attgagcaat ttcaccagcg tggaactgca
     1741 ggcattgccc gtacccacgt tgaaggcaaa cgacaccgca gcgtcataca ccttctgcgg
     1801 cggctgttgc ttcacacact tttccagcgc ccgctccaca cgcagcacgt tggagatcag
     1861 cccttctgct gcctgtcgct ccgtaatggt tttgccggga atgacgcccg acgtattacc
     1921 aataccgtcg gtccagacac ccgcgctgca ctgatacggc tgcagacgac aaccttcgta
     1981 atcggcaatc agtttcagtc cctccacgga ggtgtgaagc tgctgaaaac ccggcagcgt
     2041 ggcagcaata gccagcacgg ccccgacaag gcagcgttta acgattgatg gattcatagt
     2101 cctcccgcga gatctgcccg tcgcgcagaa gctggtaggc tttgtgtttg tagtaccagt
     2161 tgatagccag catcagcaca ccaatcatca ggccgcccag cgttgaggca tccttgatgg
     2221 acaaatcgcc cagccaggcc agcacgacgg cgatgcaata cgtgataaag gcgctgattc
     2281 gctcaagcgt cataattcag tcccatagct ggacggtctg cacggtggtg gtggtcggaa
     2341 tgtccggcag ctccacctgc agcccgtgag gtaaaaaggg gccgtattcg gcaagccccg
     2401 gatttgcctt cagtacctgc tccgtgacac cctgcgtgcg cccgtaatga cgccagcaaa
     2461 gcgcgtccac cgtgtcatac tgatgcgcac gcactttcat cagataagct ccactgtgca
     2521 gtgcggcgcg tcctgcaccc ggctgatggc ccagcgggcg tcacgccaca aatcaccgct
     2581 tgcttccgcc agttcttcgc cccgcttcgc accggatgcc gtggcgtcat agtcctggaa
     2641 acgttcgttg agcatggcgc gagcccagca gtaaaccgcg ttgaaatagt gctgaatgcg
     2701 ctcacttttc ccgtccagct gttccgccgg aacctcagcc agcgacgcat accccagcat
     2761 ctgctggcgt ctgcgaaact catacagctc tgcgttaacc tccgaaattg ccgacagtgc
     2821 aacctgcttt aaacgcggct gcgtcaccgt gccgtcagtg cgcatgacac tgcgaaactc
     2881 cgacaggtcc acatcaggcc agaacggcgt atttctaatg atttccgcct gttccggtgc
     2941 ctgttctggc gcaacaaact tcatgctgct ttctcctgaa ataaagggcg gtggacgggg
     3001 ttttgatgtg gctgtgcctt tcgccacccc gtgccgcccg tgcgcggggg cacgttctgt
     3061 cagcggctgt cattgcgcag tctgcgctcc agctgctgtt tgtctttttt cacgccacag
     3121 cggggatcga gctgtaacgc atggttgaga tgattaaggg cggaagccgg attgctttca
     3181 ctcaggaccg cgccaatcgc tttatgcaga cgtgcccgtg actggtccgg catatccaga
     3241 ccgtctgtca gctccagcgt ctgcagcaac agatcggcat caaagccggt ggcggcaagc
     3301 attgcgctct gcgctgcatc tgccatttcc tctgtcagca cggtctgcac gttgcggttg
     3361 cccagcggca tcacccatcc atgacgcagg gcatgacgcc cgatctccag cgccccggca
     3421 taatctccgg catcaatgcg ccacaacatc acgtacatca gcacgtcatc ctgttgagcg
     3481 cctccggcag ccaggacacc ctccgcccag gcggcgtact tcggcagcag ctccactttg
     3541 atttccgctt ttttgaccgt ggactgaacg cccttgagac ggcggcggtc ttccgccagt
     3601 tgcagcagca tcaggtcata gcccgatgcg tggcgaacac tgccaccctc acggacggcc
     3661 tgttcagcct gaacgcgcag gcgatgctgc cgtgcgggac tcaggctcat ggattacgct
     3721 ccggtttcgg ctgcggcggc gctgaaatca ctaatctgga tgttttccac cagtgcggcg
     3781 cagcggtagt cctcaaccac ataggcttcg ttaacggatt caaagttttc aatccggtca
     3841 cgtttcgggt tgtcgataac tgaacggcgg cgggtgtctt cctgccagta gatggacagg
     3901 ttatccagac gggtgatcag cagcgcattc ggcgggaaga acggcgcacg cacggcctgc
     3961 aggccaccca tgcgtttctg actgatgatc atatcggcag ccagtttttc actgttttcc
     4021 tgctctttgt tgaccagcgg gaaatacttg tcagacagca gttcacgacc gcaaatcacc
     4081 accagatcgt catcgtcctg gtagaccacg tcgataagct cattgacggc atccatcacc
     4141 acggcgtcca ggttggcata ttcgccacct ttcccgactt tcactgcgcc cggtgtggtt
     4201 tcaccgcccg tggtggtgct gcccatgacg tgatccggtg catcctcacg gattttctgc
     4261 agccagcctt tattcacatc ctgcagcagc gggttttcgc tacggttgga ggttttcgca
     4321 cgcttcacgc cattaaagcc gatcatgatg cggtccagtg cctgacgttt cacgatggcg
     4381 tcacggatac gcacctgaaa atcctgaaac ttcgcccaca ggtccagctt cgcgtaggtc
     4441 agcaccgtgt caaagttggt ctgctcgcat ttatattcca catcgaccat cagcgtcgga
     4501 tcgacaggtt cacgctcttt cgcagtggta tcagtggttc cggcaatggt gctgccaact
     4561 cccaacccca gcagctgacc ggactgctca gtcactggcg tgacgttaat cagagtcagg
     4621 aaagcggcgg actgctggat ctggtcttcc agcgtctgct gcacggacgg ctctacggtg
     4681 aacttgctgg acagttcttc aactgccaca ccgttcagac gcgccagttg ctgcaggtaa
     4741 gcgttaaaag caaagcgggt attcttcttc atcaggtttt gtgctccatc agcaattggt
     4801 cagagtgtca gcgggggcgt taccgcctgt tgcacgctgg cggtagtcct ggcggctgtc
     4861 ttcatgactc agcttgtcca ccagttcgtt aaaggcggtt tgctgtgcct gcagggcagt
     4921 ctccagctca gacaggcgtt cttcctgctc agacagggat ttttcggtgc gtgcgctcag
     4981 gttctgctgc tcagtggcga ccagctccac ggccttatgc acatcagaga accgggcgtc
     5041 atcggactgc tcttttttgg taaacagcgc cgtgacgcgg gcaaacaggg acggtttgtc
     5101 gtcctggact tcttccagtt cgatcaccgt ttcctctgcg gcggtaaaga gattggcggg
     5161 attctgcttg cggtttgcca gcgggttatg ggctgcactg gcgctgaatg tcagcatttc
     5221 agtgcccaga ctggcagggt catcagtggc agccaggccg actaggtagg ctttgcccgt
     5281 atcagcgaac ttcgggctga cttccataaa ggtgaataat ttctggcctt ttttcaccag
     5341 ttccaccagg gactccgttg gctcaacgtc agcatacagc gccatcttgc ctgccagcgg
     5401 accttccgtg atttcttcag caaacagcgc cgtcaccttg ccgtagcggt taaaggtgct
     5461 gtccggcaga taagacttga tgtgctcaag gttaatcagc gcggtataca ccgccgggtt
     5521 atagctggct gccatctgtt ccagccattc acgctggatt tcgcgtccgt cggtggtggc
     5581 accttccacc ccgatgcgaa aacgctttgc tttcactgtc atgagccgtg ctccgttaga
     5641 aaaaacttac tggagcctta tggttgcggt gataggggca gtgaaacaat gcgcggtatt
     5701 tgtaccgaca accacacaaa ccgcaggcgg ggaaagcctt cattcaaggc tgtaggtttg
     5761 tgccatgaac accacactga cacccgcaga tctcgatccc cgtcggcagg ccatgctgct
     5821 gtactttcag ggataccgcg tagcccgcat tgctgaaatg ctgggcgaga aagttgcaac
     5881 cgttcacagc tggaaaaaac gcgacaagtg gggtgactat gggcctctgg atcagatgca
     5941 gctcaccacc gccgcacgct actgccagct catcatgaag gagcacaaag aagggaaaga
     6001 tttcaaagag attgacctgc tggcgcgcca gtctgagcgc cacgcgcgga tcggcaagtt
     6061 taacaatggc ggcaacgaag ccgacttaaa ccctaacgtc gccaaccgca acaaaggccc
     6121 acgccgtcag cctgaaaaga atgttttcac cgatgaccag attgagaagc tggaaaaaat
     6181 tttccattcc tccatgttca actaccagcg ccactggtgg gaagccggaa aaaccaaccg
     6241 catccgcaac ctgctgaagt cacgccagat cggcgcgacc ttctattttg cccgtgaagc
     6301 cctgattgac gccctgctta ccggacgtaa ccagattttc ctttctgcca gtaaggcaca
     6361 ggcccacgtc ttcaaacagt acatcatcga ctttgccaaa gaagtggagg tggagctgaa
     6421 aggcgatccg atggtgcttc ccaacggggc cacactgtat ttcctcggca ccaatgcccg
     6481 cactgcccag agttatcacg gtaacctgta tctggatgaa tatttctgga taccgaaatt
     6541 tcaggagcta cgcaaagtgg cttccggtat ggctattcac aagaaatggc ggcagaccta
     6601 tttttccaca ccatccagtc tgacccacag tgcttatccg ttctggtccg gtgcgctgtt
     6661 caaccgtggg cgcaacaaag ctgacaaggt ggacatcgac ctgtcccaca gcaatctggc
     6721 ccccggccgg ctgtgcgcag acgggcaata ccgccagata gtcaccgtgg aagatgcggt
     6781 gcgcggcgcc tgtaacctgt tcgaccttga ccagttgcgc atggagtaca gccctgacga
     6841 ataccagaac ctgctgatgt gcgagttcgt ggacgatctc gcgtccgtgt tcccgctcag
     6901 cgaattgcag gcgtgcatgg tggacagctg ggaagtctgg accgactttc atgcactggc
     6961 cctgcgcccg tttggctggc gcgaagtgtg gatcggttat gacccggcaa aaggtacgca
     7021 gaacggcgac agcgccggat gcgtggtggt ggcaccgcca gccgtgccag gcggtaagtt
     7081 ccgcattctt gagcgtcacc agtggcgcgg aatggacttt cgcgcccagg ctgacgccat
     7141 caaaaaactg actgaacagt acaacgtgac ctatatcggc atcgactcaa ccggcgttgg
     7201 tcacggggtt tacgagaacg tgaaagcgtt ttttcctgcc gtccgggagt ttgtctacaa
     7261 ccccaacgtt aaaaacgccc tggtactcaa ggcctacgac attatcagcc accgccgtct
     7321 tgagtttgac gccgggcaca ccgacattgc gcagtcattc atggcaatcc gtcgcgccac
     7381 caccgccagc ggcaaccgcc cgacctatga agccagccgc agcgaagaag ccagccatgc
     7441 cgatctggcc tgggcaacaa tgcacgcact gtttaacgaa ccgctgcagg gcgagtccgc
     7501 caataccagc aatattgtgg agattttttg atgggaaaga gtaagaagaa ccgcgctgcg
     7561 gcgacgaaac agacccagca taaaaaccag acttcagccg aagcattcag cttcggcgat
     7621 cccgttccgg ttctggaccg ccgtgaacta ctggactatg tggaatgcgt acagacagat
     7681 cgctggtatg agccgccagt gagttttgac ggactggcgc gcaccttccg cgccgccgtg
     7741 catcacagtt caccaattgc ggtgaaatgc aacattctga tcagtaccta catccctcac
     7801 ccgctgctca gccagcaggc tttttcacgt tttgtgcagg attatctggt atttggtaac
     7861 gcttacctgg agaaacgcac aaaccggttc ggagaggtca tcgctcttga gcctgctctg
     7921 gcaaaataca cccgacgcgg attagacctg gatacctact ggtttgtgca atacggcatg
     7981 accacgcagc cgtatcagtt cacgaaagga agcatctttc atctgatgga accggacatc
     8041 aaccaagaga tctacggcct gccaggttac ctttctgcca ttccgtcagc cctgctcaac
     8101 gagtccgcca cgctgttccg ccgatagtat tacattaacg gcagtcatgc aggcttcatc
     8161 atgtacatga ccgatgccgc gcaaaaccag gaggatgtga acaacctccg caatgcgatg
     8221 aaaagcgcca aaggaccagg taacttccgc aatctgttta tgtactcgcc taacggcaaa
     8281 aaggacgggc ttcagatcat cccgttgtca gaagtcgcgg cgaaggatga gtttctgaac
     8341 atcaagaacg tgagccggga tgacatgatg gcggcgcatc gtgtgccacc gcaaatgatg
     8401 gggattatgc ctaataatgt cggggggttt ggggatgtgg agaaggcgag cctggagttt
     8461 gtacgtaacg agttaatacc gttacaaaaa agaatgatgg aaataaataa attggtaagt
     8521 gagaacattg ttaaatttga tagttataaa ctagctactt aacgtaatca ctaaatgagg
     8581 ttctttaaca cagctaaaaa atttattaat agccagcagt ttttatgaat ttcttcagcg
     8641 tgcagataca cgctgaagat gcaaaaaatc atatgagacg accgtctgga cttctgttac
     8701 ctaaatgtaa ttcacagagt atccacctaa tcacatcagg tcgaattaac ccagctacaa
     8761 aaaccgaatt ccaattattt ttacctttat gtttttcatc caacagcaac aactcgcgag
     8821 tcaacaactc atatacatcg ctataagtag aaagactctt attcagtcct ctaataacaa
     8881 gctcaggata taagtaaaac gactcccatt gttcagacaa atacccctta atattattct
     8941 tgataacaac attatcaaga tgatacttaa cattgttata ttctggtgac attgggtcaa
     9001 ttatgatttt tacatcaatt gtaggtgttc gccctaattt agaaaaatta gcacatatta
     9061 atctttgttt taatatatta tcaaaatatg gatgcagcat ccgatggcct gcagaattag
     9121 ctatagtagt acttttgaaa gtattacatt tgcaagcagg aaccaaattc ttagtgaata
     9181 aagaaaattc tggatagttt tccttgggta aaacatgatc caaagtcccg ctatgcattg
     9241 aaccacacat tggacataga gagttagaat tagctgctct tatatcatca attatagata
     9301 aacactttgc gggattgtta taataatatc tcaaagcttc agcaatattg ttactcaata
     9361 cgccacaaga attgacatta agataccctt tattctttat gtacgatcta tatgcattaa
     9421 gtataaatgt atcctgtcca ttcagaacag aatgatgttt taatctctta tttgcagata
     9481 gttttttaaa tgcagaataa tctttaaaaa caggtctttt taatttattc atttcaattg
     9541 cccatttcat ttctcaacgc agttaataca ttaagtgata agtcctcgct atatttagat
     9601 tcaacatcat cccaagacat attctttgca attatattgt tttttacttt atttaaaatt
     9661 tgagttggtt cactctcacc gaaaacaaaa tatgagatat ttcctatatt cgccccaaaa
     9721 gtactcaatc tcggtttttc gctaacaatt accccatcat ctcgtcttct gagtattgtc
     9781 acttgctcct taaaaacctc ccgaacaaga taaaccgaat gcgttgctat gatagctgaa
     9841 gagcctgtct tttctaataa attatcaagc aatgccataa atttattaat aaatgaagga
     9901 tggagatgtg tttctggctc atctattaat agaagagtcc cattctcaat atacaaacag
     9961 acttgtacac aaaacttaat aaacgatatt tccccactac tcaaagggta acatttattt
    10021 tcaataagcc tcaaaggctc gccagagaga ataatttcag aatataatga tagtctagat
    10081 tcttcgcctc gagaattaag ctcatttaat ttaacaaatg gtgaattttc accgccacat
    10141 ttgattacta aatcgttgtt actatttaga acactcagtg catcaacaaa tatattccac
    10201 ctttgattat agccgatagc gtcgttactc ctcgctagtt gcactattaa atcattcaag
    10261 tttaatttgg attttgattc acgatttaaa gtcaatcttt tgtaccatat caatgatttt
    10321 attcttttat cggttggaaa tgaacactta ctttcatttg ttggagaaaa agcaatgagc
    10381 ctactaattt gaattcgttc ttcaatgctc acaccgttag aatcaactac agccaccaaa
    10441 ttatcctttc ctttaatcgc agccaatgcg atttctctaa gcgtttggct ttttcctaca
    10501 ccattctcgc ctataattat ggaaattctt tttggtaaat catgctcgtg atcaaaatta
    10561 aaaacataag atacatcttc ttcattaact ttattcgaat agctaatctt gatgtgctgt
    10621 gataaattac ccaactcctc atattgaata ccggaaagaa ctgttcctgc atttttgaaa
    10681 gtaaaatacg actcagactc tctaatgaaa gatttattaa agatatcaga agagattgct
    10741 tttttcctca aattctctgc actagcgtca cttttcttag aaacaacatc attaatgaaa
    10801 tttaatatat atttggattc attcacccca aaaaatctta ctagtcttcg ataattattc
    10861 atatcctgaa gcatgacaaa ataatcatta aacccctcat cctttaaatt agattcttcc
    10921 tttttcttca ctcgccttat cagttctgct tttccatttc gttcctcctc tgaattcaaa
    10981 aaaccaatat aaccattcag tgaaattata tccttacact cttttttatt aaaaatagat
    11041 atttcgactc tactcaaata tccaaaatca ttccaatcgc ttcttgatgg gactattttt
    11101 atataattac cagaatcaga tatctgttga ttatttgtaa ttaaaaaata taaactataa
    11161 tttttatttt tcttgactaa cattattacc ctctcacttc attagtgatg atttattttg
    11221 gaattatttt acacacttat acctagcgcg caatcgtatc cccgccacgc ctgcccgctt
    11281 tatgtagagg ttttcatgca cctgcatgat ctacgcaaaa gcccgccagt tctggcgggc
    11341 cttagcaaaa acgatcctca aacgatcatg cgatctcatg cggcatagac atgcactaca
    11401 gagctaacgc ctcgcaaggg ctcgttgttc aaccttgctg acgccagaag ctagttcaga
    11461 cgccagcaac gtttcttaat gcagccagct gtcgtcttcc cacaccttct gcataatttt
    11521 catcacttgt tttctttctt cgtccagttg caatccagta agttccacac cgttagagct
    11581 acctttgcgg atacgaatta ccgttttggg atacagaggg cgcagattgc ggtaaagctc
    11641 ggattcaaga gcgtccaggg tagactggct aatcttctgc tctttatcga tcattatttc
    11701 aatgcgcata aaagtcacct cagctgatga catccattga gcggttgtat tcgtgggttc
    11761 tgatttttgc catgagttca tcagtcaatt cagaaaccca ctgcagagcc agccccttct
    11821 cttcatcact acactcacta gccgctacaa gcttaagaaa aaaatcaatg cgctggagct
    11881 tcaaagactc caaaaaatag tcctgcatct ttcctcctat gacaccacac gcaatactgt
    11941 atgcataacc actatttata tttacagtat ataataatct tactgatgta aaacgttttt
    12001 ttacgttcat cagcctgata tgcctggtat tattaagagc acgaattgtt aacccgcgta
    12061 attaatacag gttccgccac tgatcatctt cctgcaaacg ctggttccga tagaagatac
    12121 gcaggcctgc tcctgacgga atactgcctc cgcgaaggag taaatcgacc tctttctcgc
    12181 tgccatcaaa tcctctggac ttcagctcat acacgagctg cagtcgctga tggtctgtaa
    12241 ttcgctgttt gtagtcttta cgccgtttcg gtttcaccag gcgtaacctt gctgccagtt
    12301 cccggcgctc ttttttgctc atactgtgca ggtaatcgtg caactccttg tcatccatgc
    12361 tggtaatgtc cgttctgggg ttcccatcag ctgatttatc tttctcctgt tggttcaaat
    12421 tttcagcaag gagacagtta ttgccacgag tccaaggggc gcaagcgccc tggtcggctg
    12481 ccgcctcctg aacgtcaacg gctttacgaa ccattttcca cttcactgca tgagtgcaga
    12541 tcttgccttc tgcaatgggt gaccagatgc cataaatacg aataccgtga tcgccatagg
    12601 cggtcggctc ttcattgatt tcataagcgg ttctgatcaa gtgatatttg cgaggaacca
    12661 gcacgccgcc ctgcctcata atgtaggtgg caaaacagcc agcatcagca gcagccagga
    12721 tggcatcaag gcgcgggtta tccagtaccg gcgcacctgc ttttttgtca ccctgctgcc
    12781 ttgccgcctg accagccaac aaccgcagtt cacggtaagc ctgacgccct ggaataccaa
    12841 agaagcggaa ttgctgaaca cgatgcagag acgcccaggg attaacgtat tcagcgttat
    12901 cacgcaggga tttacccgtt tccttgctga tctcgccagc cagaccacgc ccgtcaatgt
    12961 tcttactgat gtatttcgcg atgtagcttg tcggcgttcc tttgcgcggg tttatcagct
    13021 cagatttaaa gcgtggacca gtgttattac ccagttcctc gcggtcttca cggatggcaa
    13081 acttacgcaa caatgcagta atggcgcggc ggtctttttt gcgcataaaa cacaacaggt
    13141 gccagtgaac tgtaccgtca tgatgcggct cagccacccg cacgccatac cagcgcaacc
    13201 cggctttgtg catcgcctta cgaaatgcag caaacatgcc gaccagataa tcgctgcttt
    13261 gtcttaccgt cgcatttgtc caggttgggt ttggtctgcc gttattgagc gtggaatgga
    13321 aacgtgacgg acaggtaatg gtgtagaaaa cggcgcagtc accgcgcatt tccgcgataa
    13381 gctccagacc tttaacacag gccatcatct cattgcggcg gtgcgccggg ttgctgctgc
    13441 tggcgtttac cacgtcttcc atatccagcg tgtcgccctc ttcgttcacc agttcatgag
    13501 aacggaaaaa ctccaatgac ttgcggcgct gctcacgttt atgcatcacg gcttcatagc
    13561 tgacataggg agatgctttt ttgctgacca ggcagacagc acgcaactgc tcttcccgcc
    13621 attcgcaacg cattttccat aatttccggt accaccagtc ggcgcacaac atacgcgcca
    13681 gcgaacccgg aatgagttca tagggcacgg gtttacggcg gtttcttttc cgacggagtt
    13741 gctcaaacgc aggcgggatg acatccagac gcagggtttc cgctgccacc ttttcccatg
    13801 tcttgcggat ttcttctggc ttaacgtcat cggtggcata caaatcacca caagctgcat
    13861 caaggcacat gctcatatgc gcagcgacaa gggtagacag gcgtttcacc tgatcctgac
    13921 tcatttcagg cagaatcagc aggccgtcca gccctacatg gcttgccata aaacgaaaag
    13981 atgcagatag ctggctgtcg cgtacatgct ccagtcgttc cagacatggc ttaatcgtct
    14041 cacgtaaata gcgggaataa gcctttggcc tgcccaggct gctgaagtat tcaatacgtt
    14101 gcatcagcgg cttgctgata tgggaaggct gggcgttgac gtccgccaga atgaccatat
    14161 ccggattaaa acgctgctgc tcatgcgcca gctttgcccg gctaatgagc ttatcctgct
    14221 ccatttcgtg ctggacagga tcacgtgatt cataaaaaaa ataacgctcc cagacctgat
    14281 cactcagtgc ctcgcggcgc aactgttcct gctcgttatc ggcagcgtac agagtgatca
    14341 ggtttgaaag cgcagaaacc ggcgcaactt ccgccgggtc cagataaggg ttaatggcct
    14401 ttttcgggct gttccatgag aacgctgcgg cagcctcgtt aaagccgcag cagttgttca
    14461 tatcggcatg gctcatgcac gtactccgta cacggcagaa ctatccacgc cacgcgaata
    14521 atcaaatccc acccagcagc gcggcccgga aacagcaatg atttctgttg ctgatttacc
    14581 ctcgccagct gccacaccga tgctgcgttt taccttgata tagtggtgag taaaattgcg
    14641 atacagcgaa cggatcaggg atgtgtcact gttagaaaca atgaccggat gtccttctga
    14701 tgaccgatgt tcaagaacgg atgctaggtg atactggtca tcttcagtga aaccatcagt
    14761 gtgatagccg gaaaacgtac cgtcatacgg tggatcgcaa tacaccacat cccccgcctt
    14821 caacatcgcc agcgtttcat caaagctggc gcagataaac gttgctcgct gggcttt
//
LOCUS       DAJVTY010000101        14633 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVTY010000101 DAJVTY010000000
VERSION     DAJVTY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14633
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.101"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ66_002704"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ66_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1905577.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQQ66_002705"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQQ66_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1905578.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQQ66_002706"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQQ66_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1905579.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQQ66_002707"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQQ66_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1905580.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQQ66_002708"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQQ66_002708"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1905581.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQQ66_002709"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQQ66_002709"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1905582.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQQ66_002710"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQQ66_002710"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1905583.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQQ66_002711"
     CDS             complement(4598..5320)
                     /locus_tag="OQQ66_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1905584.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQQ66_002712"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQQ66_002712"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1905585.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQQ66_002713"
     CDS             6520..6738
                     /locus_tag="OQQ66_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1905586.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQQ66_002714"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQQ66_002714"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1905587.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQQ66_002715"
     CDS             complement(7472..7672)
                     /locus_tag="OQQ66_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1905588.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OQQ66_002716"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OQQ66_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS1905589.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OQQ66_002717"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OQQ66_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1905590.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="OQQ66_002718"
     CDS             10169..12082
                     /locus_tag="OQQ66_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1905591.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="OQQ66_002719"
     CDS             12082..13104
                     /locus_tag="OQQ66_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1905592.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACTYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="OQQ66_002720"
     CDS             13098..13316
                     /locus_tag="OQQ66_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1905593.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="OQQ66_002721"
     CDS             13370..14239
                     /locus_tag="OQQ66_002721"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1905594.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14557)
                     /locus_tag="OQQ66_002722"
                     /pseudo
     CDS             complement(14294..14557)
                     /locus_tag="OQQ66_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT         3612 a         3994 c         3602 g         3425 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggttcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttatcgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcaaggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcaa caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caagcccaga gccgcattaa gatgcttgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg
    12301 cgtttagtga tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtacctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgcc ccgttacttt attcgtaccc
    14581 cttataatgg ggtgttagcc agccagaccc ggcatgatta ctgcccccag tcg
//
LOCUS       DAJVTY010000102        14341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVTY010000102 DAJVTY010000000
VERSION     DAJVTY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14341
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.102"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..958
                     /locus_tag="OQQ66_002723"
     CDS             <1..958
                     /locus_tag="OQQ66_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905595.1"
                     /translation="TKSLALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDT
                     DSTGSLLGASEDGEIGSSSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSV
                     CDSELFQDYVHSSITNRVKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLE
                     TTHTELGKLIPKITHIVRRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIK
                     AEAKPSLNEVQEIIEKYSAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYS
                     TYSAAYIYEQISHKRKEFISPILKELKALQSQPRIKKASGED"
     gene            959..1495
                     /locus_tag="OQQ66_002724"
     CDS             959..1495
                     /locus_tag="OQQ66_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905596.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            1446..2456
                     /locus_tag="OQQ66_002725"
     CDS             1446..2456
                     /locus_tag="OQQ66_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905597.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(2511..3776)
                     /locus_tag="OQQ66_002726"
     CDS             complement(2511..3776)
                     /locus_tag="OQQ66_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1905598.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(3940..4024)
                     /locus_tag="OQQ66_002727"
     tRNA            complement(3940..4024)
                     /locus_tag="OQQ66_002727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3988..3990),aa:Leu,seq:caa)
     gene            4220..5239
                     /gene="ahr"
                     /locus_tag="OQQ66_002728"
     CDS             4220..5239
                     /gene="ahr"
                     /locus_tag="OQQ66_002728"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1905599.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            5369..6871
                     /locus_tag="OQQ66_002729"
     CDS             5369..6871
                     /locus_tag="OQQ66_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1905600.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKEV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(7032..8114)
                     /gene="lptG"
                     /locus_tag="OQQ66_002730"
     CDS             complement(7032..8114)
                     /gene="lptG"
                     /locus_tag="OQQ66_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1905601.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8114..9214)
                     /gene="lptF"
                     /locus_tag="OQQ66_002731"
     CDS             complement(8114..9214)
                     /gene="lptF"
                     /locus_tag="OQQ66_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1905602.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9481..10992
                     /gene="pepA"
                     /locus_tag="OQQ66_002732"
     CDS             9481..10992
                     /gene="pepA"
                     /locus_tag="OQQ66_002732"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1905603.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFEQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            11152..11595
                     /gene="holC"
                     /locus_tag="OQQ66_002733"
     CDS             11152..11595
                     /gene="holC"
                     /locus_tag="OQQ66_002733"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1905604.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11595..>14341
                     /gene="valS"
                     /locus_tag="OQQ66_002734"
     CDS             11595..>14341
                     /gene="valS"
                     /locus_tag="OQQ66_002734"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1905605.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FV"
BASE COUNT         3783 a         3457 c         3692 g         3409 t
ORIGIN      
        1 aactaaatct cttgcactca cgataccatg ggatccaaaa gaaaaaccaa acagttcaaa
       61 gtgttattgc aaggatattt ataaggatga aaatactggt gatttcttag tcatgttatg
      121 gaaatctgat actgatagta cgggctcttt attaggagct agtgaagacg gtgaaatcgg
      181 aagctcatcg gtggttaaat atactaatag ctacagaggg aaaaaagtta tctgggggcg
      241 gccatgcttt tattgggtta tacctgaact tgaaactata gtatccatta aattcgacca
      301 ttctgtttgt gattctgaac tatttcaaga ctatgtacac tcatctatta ctaatagagt
      361 taaacatagc aaaagggtta aaaataaaac cgagaaaggt tatataaggc tatctaatac
      421 cgatgacgaa gacttatata aatatatgta caggtttgat atgaagctca gaagcttaga
      481 aaccactcat actgagttag gtaaactaat accaaaaata acacacatag ttcgcaggga
      541 aactattatt ataaatccaa atgatgcacg agctgattgg ttaaagacct ttagcactct
      601 cgttccattt gtgtccggaa agaaaaatgc caaaacaaga caaattgaaa taaaagcaga
      661 agcaaaacca tccttgaatg aagttcaaga aatcattgaa aagtattcgg ctgaggatag
      721 agaaaaacgt ctttgggaca atgttggatt tgctacagac aagggtataa cttgggtgga
      781 taaatataga atgcgtgata ttctcaatgt cccttctgac gactattcaa catattccgc
      841 cgcatatatt tacgaacaaa tatcacacaa aagaaaagaa tttatttccc ctatattaaa
      901 agaattaaaa gcattacaat ctcaaccaag aataaagaaa gcatctggag aggattgaat
      961 attgaaagct atagcaggca tagccaaata cacatggata tacatggcga tattcattat
     1021 atcgctgatg attatttcgt catccgatat taactatgtg tataacgatt ttgccaacta
     1081 ttgcactctt ctcgtatcag tttctggaat ggtttttact attatgggaa tttggttagc
     1141 gtttctttac ccaaatgctt tgcagcgtat tgtgaacccc aaaactatag aaaccgttga
     1201 tttcaccgaa caactggaag atacaagaag attagaagca atagttggaa gtgttctaaa
     1261 atcatctttc gttatgatta ctttacttgt tgtatactta gttaaactag tgtttttcaa
     1321 aagtcatctt tatttagaga gccaagatgt aataaaaaac acatctttag cattaattat
     1381 tgctatttcg tacatgcaga ttgaggcagt aattagcgta atacttgcaa atgttatgtt
     1441 tatcaatgat ttacataaca agcgagagca aagagacatt gatgatcaag tatgagggaa
     1501 aaatgaaaaa gaatatacgt gttataccta aaaatattca tagcaagtta agaaggcttg
     1561 gtaatacagt tgttgctggc acttctattg catttacgga aaaccaatta aaaagtggag
     1621 cattagagca ccttggaata tattttgata atggtgttaa tgcttatgag acgtcggtca
     1681 taccagatcc attgcagggt aaatattcac ttaaaaatgt ttttggtgaa gaaattgtca
     1741 gaaaagacct cccaaaagaa actcattaca ctgaaattga gtcacctaac tggggtgata
     1801 gctctaatgg tacacatact gttagattac cttacgagaa atatcccaga gacattatac
     1861 caccaacact catagccatt gaaattaatc acaaacagcc ctcggacagc catttcatat
     1921 ttaacttcag agtaactaga attttagaca aacaatcaaa taagtttgat gatgaattat
     1981 tatttgattt aaatcttctt caagaaaatc ttggtaaatg tggaattgag gatgctgata
     2041 aacccatatc gacttatgct gatacactta tagtctcatg ggaaatattt ccaccaggta
     2101 gtaaagaaga aactttagca agaattttta gaggtaaaaa tatcacttcc gacaagaaaa
     2161 atgtcgctga aaatagatat gacttcttca tgagtcttga acctaaaaaa atcgtcacag
     2221 gaaacagcac gttcagtaat tatattggag ctatgctgga ggacgatttg gttgtatttg
     2281 aaaacataga atatggtaat gctatatata tcctctatga taactgggat gatatcagca
     2341 agctatctcg aattgactta ttatcaggaa gagcaggaag taatttcgat agaataatcc
     2401 atagtggcaa ttggaaagat gaagtacgaa aaaaagttgc tgctggaaga ttatgatcaa
     2461 agtgtttaat acagaaataa acgcctatat attataggcg tttattttca ctatcgactt
     2521 ttgtttattt tagcaaaatc ataaggtgtt attgttttgc ccttatttgc ttggagataa
     2581 tcagcccacc attgaaccat caatcgacgt tcatccaaat gttcagaggt gtgaatataa
     2641 gcagcacgta cattattacg ctctgaatgg ctcaactgtc gttctatagc atcttcactc
     2701 cataatccgg actcgcccaa cgcaccacgg gccatcgttc taaacccgtg cccgcaaact
     2761 tcggttttcg tgtcatagcc catcgcacgc aatgcgctat ttaccgtgtt ttcgctcata
     2821 accttagttg cgtcatgatc acccggaaaa agcagctctt tatctccact aatctgcttt
     2881 aactggttta gcaaaatcat cgcctgccga ctaagctgaa cgatatgctc ctctttcatc
     2941 ttcatgccac ggtacgagta acgcacacct ttaatttctt ctcgttttgc aggtactcgc
     3001 cagagagatt tatcgaagtc gaactcatcc caacgcgcga aacgtaactc actggaacgc
     3061 acaaaagtta gtaaggaaag ctcaaccgcg atccgcgtca ttacacggcc acgatatgca
     3121 gcaagacgag caagaaactc agggaaccgg ctggagggca aagcggggta atgtcgcgct
     3181 ttggttgtcg atagcgcacc ggccatatca ctggctggat ttgagtcgat gtaatcgttc
     3241 tgtactgcat aacgcataat agccgtgaca cgctgctgaa ggcgctgtgc aacgtcatgc
     3301 ttaccactgg catcaacttt tttaatcggg gctaataggt ggctagtttt gagctgacgg
     3361 atgtcagatg aaccaatatg aggaaagata taaagctcaa gataacgtag aacgcgtgat
     3421 cggtgatctt cactccagcg cttattacta gcatgccatt cacgagcgat agtttcgaaa
     3481 gaatatgccc ccgaattctc ggcctgagct tctttctgtt cggcttttgg atcaatgccc
     3541 tgcgctaaca gctttttagc ttgatcgcgc tttgctcttg cctgagcaag cgtcacagta
     3601 ggccaaacac caaacgcgag acgatcctct tttttgtcag agggacgtct gtatttcatg
     3661 cgccagtatt tggaaccttt ggccgaaacc tcaagataca aaccaccacc atcggccatt
     3721 ttgtaggttt tgtcttttgg ctttgcggtc tcgacctgtc tggcgttgag cttcatttgg
     3781 gggcacattt ctaatcgaag ttaagatgcc cccaattatg cccccaatga tatccggatt
     3841 tcaacggaca acctcggaag acgcaggacg aaaaaatcgc tgcaagcatt gattttaaag
     3901 ggatatttgg actttctcgg atggtcttgg aagtaagaat ggtgccgaag gccggactcg
     3961 aaccggcacg tatttctacg gttgattttg aatcaactgc gtctaccgat ttcgccactt
     4021 cggcactgaa gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg ccaccatgca
     4081 agcgccagct tgctcgcacc tcgctaagtg ctgaaatttt cagcattgca cacagcgatg
     4141 tgtaaccttt gtcacactcc aggcaccccg ccctgccatg ctctacactt cccaaacaac
     4201 accagagaag gaccaaaaaa tgtcgatgat aaaaagctat gccgcaaaag aagcgggcgg
     4261 cgaactggaa gtttatgagt acgatcccgg tgagctgagg ccacaagatg ttgaagtgca
     4321 ggtggattat tgcgggatct gccattccga tctatcgatg atcgacaatg aatggggatt
     4381 ttcacaatat ccgctggttg ccgggcatga ggtgattggg cgcgtggtgg cactcgggag
     4441 cgccgcgcag gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct ggacggcgcg
     4501 tagctgtggt cactgcgacg cctgtattag cggtaatcag atcaactgcg agcaaggtgc
     4561 ggtgccgacg attatgaatc gcggtggctt tgccgagaag ttgcgtgcgg actggcaatg
     4621 ggtgattcca ctgccagaaa atattgatat cgagtccgcc gggccgctgt tgtgcggcgg
     4681 tatcacggtc tttaaaccac tgttgatgca ccatatcact gctaccagcc gcgttggggt
     4741 aattggtatt ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa tgggatgcga
     4801 ggtgacggcc tttagttcta atccggcgaa agagcaggaa gtactggcga tgggtgccga
     4861 taaagtggtg aatagccgcg atccgcaggc actgaaagcc ctgtcggggc agtttgatct
     4921 cattatcaat actgtgaacg tcagcctcga ctggcagcct tattttgagg cgctgaccta
     4981 tggcggtaat ttccatacgg tcggtgcggt tctcacgccg ctgtctgttc cggcctttac
     5041 gttaattgcg ggcgaccgca gcatctctgg ttctgctacc ggcacgcctt atgagctgcg
     5101 aaagctgatg cgctttgccg cccgtagcaa ggttgcgccg accaccgaac tgttcccgat
     5161 gtcgaaaatt aacgatgcca tcaagcatgt gcgcgacggt aaggcgcgtt accgcgtggt
     5221 tctgaaagcc gacttctgat ccctttgccg ggtgcctgtc gcccggcact taaatctctt
     5281 tggaaggcgt ctcgcaatct gctgaattta gcggtgaaga gtgcgcagcc gttgcctata
     5341 ctccaggcag gaaactggag gaaatctcat gagcgaaccc ctattaattg cccgcacgcc
     5401 ggacacagaa ctttttttac tgccgggaat ggctaaccgt cacgggctga ttactggcgc
     5461 aacggggacg ggtaaaaccg ttacgctgca aaaactggca gagtcattgt cggaaatcgg
     5521 cgtgccggtg tttatggctg atgtgaaagg cgatctgacc ggtatcgcgc aggcaggaac
     5581 ggcgtcggaa aaactgctca caaggcttaa aaatatcggc gtcaatgact ggcaaccgca
     5641 tgctaatccg gtggtggtgt gggatatctt tggcgagaaa ggccatccgg tgcgggcgac
     5701 ggtttcagac ctggggccgc tgttgctggc gcggctgttg aatctcaacg atgtgcaatc
     5761 tggcgtgctg aatatcatct tccgtattgc tgacgatcag ggattgttgc tgctcgactt
     5821 taaagatctg cgggcgatta cccagtacat cggcgataac gccaaatctt tccagaatca
     5881 gtacggtaat atcagtagcg catcggttgg tgccatccag cgcggtttac tgtcgctgga
     5941 aaagcaaggt gcggcgcatt tctttggcga gccgatgctg gatatcaaag actggatgcg
     6001 caccgatacc aacggtaaag gcgttatcaa tatcctcagc gccgagaagc tttatcagat
     6061 gccgaaactg tacgccgcca gcctgctgtg gatgctttca gagttgtatg aacaattgcc
     6121 ggaagcgggc gatctggaga aaccaaaact ggtgtttttc ttcgacgagg cacatctgct
     6181 gtttaacgat gcaccgcagg tactgctgga taagattgag caggtaataa ggcttattcg
     6241 ctcaaaaggc gtgggcgtct ggttcgtttc gcaaaacccg tctgatattc cggataatgt
     6301 gctcgggcag ctcggtaatc gcgttcaaca cgctttgcgg gcttttacgc ccaaagatca
     6361 gaaagaggta aaagctgcgg cgcaaaccat gcgggccaat ccggcgtttg ataccgaaaa
     6421 ggcaatccag gaactgggga ccggcgaggc gttaatctcg tttctcgatg caaaaggaag
     6481 tccttctgtg gtggaacggg cgatggtgat tgcgccttgt tcgcggatgg gaccggtgac
     6541 ggaagatgag cgcaatggct tgattaacca ctccccggtg tatggcaagt acgaggatga
     6601 cgtggaccgg gaatccgcct atgagatgtt gcaaaaaggc tttcaggcca gtaccaagca
     6661 gcaaaataat cctcccgcga aagggaaagc ggtggcggtg gatgacggta ttcttggtgg
     6721 attgaaggat attttgtttg gcactaccgg accacgcggc gggaagaaag atggtgtggt
     6781 gcaaacaatg gcgaaaagcg ccgcccgcca ggtgacgaat cagattgtac gtgggatgtt
     6841 ggggagtttg ctggggggaa gaagaaggta agtcaggcga gatttgtctt tgcctgatgc
     6901 gacgctggcg cgtcttatca tgcctacacc actgcaatat attggatttt catgattttt
     6961 caggcatgaa cgaagcacac tttgctaaca ataataaagg gagctttcgc tccctttatt
     7021 cgttcattcg gttacgattt tctcattaac agccacaggc tgattaagaa gaagctggcg
     7081 cttggcaaca gtgcgccgat gatcggcggg atgccataaa ccaacgtcag cgggccgaag
     7141 atctggtcca gtacgtagaa gacaaaaccg aagctgatac cggtgaccac acgcacgccc
     7201 atcggtacgc tacgcagtgg gccaaagatg aacgacagcg ccatcagcat catcaccgcc
     7261 acggatagcg gctggaagat tttgctccac atattgagct gataacgtcc ggcatcctga
     7321 ccgctcgact tcagatactt cacgtagttg tgcaaaccgc tgattgagag tgcatccggg
     7381 tccagcgcca ccacgcccag tttgtccggc gtgaggttgg ttttccaggt gccgctcacc
     7441 gtctgcgaac cggtgatctg tttcggattg gtcagatcag attcatcaac ctgcgacaga
     7501 cgccagactt tatgttccgg gtcaaacttc gcagtagcgg catagcgtac ggattgcaga
     7561 cgacgattct cgttaaaggc ataaatactg atgccaccta actcttcgtc acctttaacc
     7621 cgctcaatgt aaacgaagtt gttgccatct ttcgcccata agccttgctg ggtagagagc
     7681 aacgagccgc cgtacatcgc ctgcgcacgg tagttacgcg ccatctgctc gccctgcggc
     7741 gcgacccatt caccaatcgc catcgtcagc aagaccagcg gaatggcggt tttcatcacc
     7801 gacagcgcaa cctgcatacg ggtgaaacca gaagcctgca tcaccaccag ttcgctgcgc
     7861 tgcgccagca tcccaagacc aagcaacgcc ccaagcagag ccgccatcgg gaagaatgtt
     7921 tgcacatctt tcggcacgct cagcaaggta tacattcctg cgcctaacgc gtcgtaactc
     7981 ccctgcccgg cttttttcag ctgatcgaca aatttgataa tgcccgacag cgacaccagc
     8041 atgaacagcg tcatcatgat ggtggtgaaa atagttttac cgatatagcg gtcaagtacg
     8101 ccaaaaggtt gcatcacacc gctcctttac gcgaaaaact ggcgcgcagg cggcggaccg
     8161 gcacggtgtc ccaaaggttg agaacaatcg ccaaagccag ataaatcagg ttaacggtcc
     8221 acatccacag cgtcgggtcc agcttacctt taccgccgtt cgatttcagg gaggtctgga
     8281 tcaggaagaa aagtagatac agcagcatgg ctggcagcat cgatagtacg cgtccctgac
     8341 gcgggttaac cacgctcagc ggtacgacca taagtgccat cataaacacg gtgaatacca
     8401 acgtgatacg ccagttcagt tctgcgcggg cacgatcggt gtcagtgttc cacaatgtac
     8461 gcatgtccat ctggtcggta tcgttcgggt cgagcgctac cgcctggtga ccaatgatcg
     8521 cctgataatc ctggaagtcc gtaatgcgga aatcacgtaa cagtgcagtg ccttcgaagc
     8581 gcgttccctg gttgagagtg acgacctggg agccgtcgcg cagctgggtt aaatgtccgg
     8641 aatcggccac caccacagaa ggacgtgcat taccttttgg tcgaatttgc gcgaggaaca
     8701 catctttgaa atcgctgccg tcaacgcttt cgatgaacag caccgagctg ccattagtcg
     8761 cttgctggaa ttgcccttgc gccagcgccg ccatgccagg gttcgctttc gcttctgcta
     8821 acacttcatc ctgatgacgc gatgaccacg gtcccgccca catcacgtta accgccgcga
     8881 cgattgcagt gaataccgca aggatcattg ccgctttcac cagaaccgct ttgctcaggc
     8941 cgcaggcatg cattaccgta atttcacttt cggtatacag tttgcccagc gtcatcagca
     9001 gcccgaggaa caggcttaat ggcaggataa gctgcgccat ttccggcacg cccaacccga
     9061 gaagggagag caccagattc gccggaatat cgccgtcaac cgctgcgccg aggatcctca
     9121 ctaacttttg acagaagaag atcaaaagca agatgaagag tatcgccagc tggcttttga
     9181 gcgtctcccg caccagatat cttatgatta tcactttaaa tacgcccgta aaaactcgtc
     9241 ttttgcagga ttttagcttg tttcatggct taaacgtcat ttattctctt gagtcgtcga
     9301 aatcgtcgct aagataatta tactcaacgg attcacctct cagattttgt tctgacgtgc
     9361 caatgccgta ataacgttaa gattaacacg aagtcatcgc aacagcggac atgagttacg
     9421 aaagcttgca attctatctg tagccaccgc cgttgtcttt aagattcagg agcgtagtgc
     9481 atggagttta gtgtaaaaag cggtagcccg gagaaacagc ggagtgcctg catcgtcgtg
     9541 ggcgtcttcg aaccacgtcg cctttctccg attgcagaac agctcgataa aatcagcgat
     9601 gggtacatca gcgccctgct acgtcggggc gaactggaag gaaaaccggg gcagacattg
     9661 ttgctgcacc atgttccgaa tgtactttcc gagcgaattc tccttattgg ttgcggcaaa
     9721 gaacgtgagc tggatgagcg tcagtacaag caggttattc agaaaaccat taatacgctg
     9781 aatgatactg gctcaatgga agcggtctgc tttctgactg aactgcacgt taaaggccgt
     9841 aacaactact ggaaagtgcg tcaggctgtc gagacggcaa aagagacgct ctacagtttc
     9901 gaacagctga aaacgaacaa gagcgaaccg cgtcgtccgc tgcgtaaaat ggtgttcaac
     9961 gtgccgaccc gccgtgaact gaccagcggt gagcgcgcga tcctgcacgg tctggcgatt
    10021 gccgccggga ttaaagcagc aaaagatctc ggcaatatgc cgccgaatat ctgtaacgcc
    10081 gcttacctcg cttcacaagc gcgccagctg gctgacagct acagcaagaa tgtcatcacc
    10141 cgcgttatcg gcgaacagca gatgaaagag ctggggatgc attcctatct ggcggtcggt
    10201 cagggttcgc aaaacgaatc gctgatgtcg gtgattgagt acaaaggcaa cgcgtcggaa
    10261 gatgcacgcc caatcgtgct ggtgggtaaa ggtttaacct tcgactccgg cggtatctcg
    10321 atcaagcctt cagaaggcat ggatgagatg aagtacgata tgtgcggtgc ggcagcggtt
    10381 tacggcgtga tgcggatggt cgcggagcta caactgccga ttaacgttat cggcgtgttg
    10441 gcaggctgcg aaaacatgcc tggcggacga gcctatcgtc cgggcgatgt gttaaccacc
    10501 atgtccggtc aaactgttga agtgctgaat accgacgctg aaggccgcct ggtactgtgc
    10561 gacgtgttaa cttacgttga gcgttttgag ccggaagcgg tgattgacgt ggcgacgctg
    10621 accggtgcct gcgtgatcgc gctgggtcat cacattaccg gtctgatggc gaaccataat
    10681 ccgctggccc atgaactgat tgccgcgtct gaacaatccg gtgaccgcgc atggcgctta
    10741 ccgctgggtg acgagtatca ggaacagctg gagtccaatt ttgccgatat ggcgaacatt
    10801 ggcggtcgtc ctggtggggc gattaccgca ggttgcttcc tgtcgcgctt tacccgtaag
    10861 tacaactggg cgcacctgga tatcgccgga accgcctggc gttctggtaa agcaaaaggc
    10921 gccaccggtc gtccggtggc gttgctggca cagttcctgt taaaccgcgc tgggtttaac
    10981 ggcgaagagt aattgcgtca ggcaaggctg ttattgccgg atgcggcgtg aacgccttat
    11041 ccgacctaca cagcactgaa ctcgtaggcc tgataagaca caacagcgtc gcatcaggcg
    11101 ctgcggtgta tacctgatgc gtatttaaat ccaccacaag aagccccata tatgaaaaac
    11161 gcgacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    11221 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    11281 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    11341 tttgttccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    11401 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    11461 gcagattttg ccaccgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    11521 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    11581 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    11641 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    11701 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    11761 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctgtggc
    11821 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    11881 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatttggg
    11941 aatggaaagc ggaatccggc ggcacgatta cccgtcagat gcgccgtctc ggcaactccg
    12001 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    12061 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    12121 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    12181 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    12241 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    12301 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    12361 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    12421 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    12481 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    12541 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactgg
    12601 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcgctcggc ctgctggaag
    12661 aaattaaacc gcacgacctg accgtgcctt acggcgaccg tggcggcgta gttatcgaac
    12721 cgatgctcac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgagg
    12781 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    12841 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    12901 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    12961 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    13021 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac accgacgcac
    13081 tgcgccagtt ccacccaacc agcgtgatgg tatctggttt cgacatcatt ttcttctgga
    13141 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    13201 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    13261 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    13321 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    13381 agcgcaccga gaaacagttc ccgaacggca ttgagccgca cggcaccgat gccctgcgct
    13441 tcaccctggc ggcgctggct tctaccggtc gtgacatcaa ctgggatatg aaacgcctgg
    13501 aaggctaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    13561 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    13621 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    13681 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgcgactggt
    13741 atctggagct gactaagccg gtaatgaacg gtggaaccga agccgaactg cgcggtactc
    13801 gccatacgct ggtgactgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    13861 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    13921 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggctg
    13981 acaccgagtg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaaca
    14041 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    14101 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    14161 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    14221 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    14281 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    14341 t
//
LOCUS       DAJVTY010000103        14330 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVTY010000103 DAJVTY010000000
VERSION     DAJVTY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14330
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.103"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..723)
                     /gene="fimH"
                     /locus_tag="OQQ66_002735"
     CDS             complement(<1..723)
                     /gene="fimH"
                     /locus_tag="OQQ66_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1905606.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQG"
     gene            complement(743..1246)
                     /gene="fimG"
                     /locus_tag="OQQ66_002736"
     CDS             complement(743..1246)
                     /gene="fimG"
                     /locus_tag="OQQ66_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1905607.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1259..1789)
                     /gene="fimF"
                     /locus_tag="OQQ66_002737"
     CDS             complement(1259..1789)
                     /gene="fimF"
                     /locus_tag="OQQ66_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1905608.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1799..4435)
                     /gene="fimD"
                     /locus_tag="OQQ66_002738"
                     /pseudo
     CDS             complement(1799..4435)
                     /gene="fimD"
                     /locus_tag="OQQ66_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4502..5227)
                     /gene="fimC"
                     /locus_tag="OQQ66_002739"
     CDS             complement(4502..5227)
                     /gene="fimC"
                     /locus_tag="OQQ66_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1905609.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5264..5803)
                     /locus_tag="OQQ66_002740"
     CDS             complement(5264..5803)
                     /locus_tag="OQQ66_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1905610.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5868..6416)
                     /gene="fimA"
                     /locus_tag="OQQ66_002741"
     CDS             complement(5868..6416)
                     /gene="fimA"
                     /locus_tag="OQQ66_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1905611.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6893..7489)
                     /gene="fimE"
                     /locus_tag="OQQ66_002742"
     CDS             complement(6893..7489)
                     /gene="fimE"
                     /locus_tag="OQQ66_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1905612.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(7967..8569)
                     /gene="fimB"
                     /locus_tag="OQQ66_002743"
     CDS             complement(7967..8569)
                     /gene="fimB"
                     /locus_tag="OQQ66_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCS1905613.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10025..10741
                     /gene="nanC"
                     /locus_tag="OQQ66_002744"
     CDS             10025..10741
                     /gene="nanC"
                     /locus_tag="OQQ66_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1905614.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10761..11867
                     /gene="nanM"
                     /locus_tag="OQQ66_002745"
     CDS             10761..11867
                     /gene="nanM"
                     /locus_tag="OQQ66_002745"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1905615.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            11932..12912
                     /locus_tag="OQQ66_002746"
     CDS             11932..12912
                     /locus_tag="OQQ66_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1905616.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRGK"
     gene            complement(12920..13042)
                     /locus_tag="OQQ66_002747"
     CDS             complement(12920..13042)
                     /locus_tag="OQQ66_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1905617.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13124..13996
                     /locus_tag="OQQ66_002748"
     CDS             13124..13996
                     /locus_tag="OQQ66_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1905618.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14041..14220
                     /locus_tag="OQQ66_002749"
     CDS             14041..14220
                     /locus_tag="OQQ66_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905619.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4016 a         3306 c         3134 g         3874 t
ORIGIN      
        1 gccctgcgct ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg catctgcggt
       61 tgtgccggag aggtaatacc ccaggttttg gcttttcgca caataaacgg taagaggaat
      121 tggcactgaa ccagggtagt ccggcagagt aacggtgaca tcacgagcag aaacatcgca
      181 gccgccagta ggcaccacca catcattatt ggcgtaaata ttccacacaa actggaaatc
      241 atcgctgtta tagttgttgg tctgtcgcaa aataagcacg gcaattaatg agccagcttt
      301 aatcgccacc ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg gccacggctt
      361 atccgttctc gaattataaa caacccgcgg cgtttcgctg gtggtcggaa atggatagct
      421 actgccacta tattttacgg tcccggaaaa attagataac acgccgccat aagccgagcc
      481 tcgttgcagt gtgacatagt ctgtaatggt ttccggataa tcgttatggc aaaagatttg
      541 cgtcgaaaga tctacgacca ggttttgccc cacattcacg acaggcgcaa ggtttacata
      601 aacattagcg ctgccaccgc caatagggat agcagtacca ttggcggttt tacaggcgaa
      661 tgaccaggca tttaccgacc agcccatcag cagtacagca aacagggtaa taactcgttt
      721 cattacaatt atctcttcgg gttcagctgt aggtataggt gatgctaatc actgcctgaa
      781 tggttccctg agtggctccg ccatttaccg tcaatgctct gacctgtaac gggaagtgcg
      841 ctgattgtga ggaatcatcc acctgaactg ttttggtttc gccagtattc aatgtgttgc
      901 cactgtcatc ctgtagctct aactggatgt tttgcgcggt cccctggttt ttataatatc
      961 cggtactgtc ggctgccccg ctgaagctgg cagtgaccct cgacgttccc accggacaat
     1021 tagtcaactc aagcgcaaca tcatgccagg ccgatgccgc cccggcagac atcagactga
     1081 aagaataaag atcgccgaga tcaaccgtgg cattggtggt ggaaactgtg cacggtttgg
     1141 cgacgacctt accgttcacc gtgatggtga catcggctgc ctgtattgtc gcacttgcga
     1201 gcgccaatat tgccgccaat aaatacccac gtttgcgcca tttcatgagc atctccagtt
     1261 actgatattc aagagtgaag gtagccgtgg cattgatatg ccccgcagtg acaggcacct
     1321 gtgtcgccat tagccgggcg taaaaattca acgtatttgg tttacccggc gtcagggtcg
     1381 tccacgaaat cgcggacgat ggagcattaa ggggtatctg attttgctgc tcattcagaa
     1441 gctgtattcc cagtcccgaa gccgctgaca ccgtattttc aagtgcaagc aggttggcat
     1501 tgtggctatc tgcaacgccg gtaaacccaa cctttacggc agaaacggcg ttaccacagg
     1561 gtgacagcaa aatacgaaat ggaacgacag gagtcgttgc gccaatgttg ttaaattgct
     1621 tcgccgcgtt ttccatcaga tcaacagtaa aattggttga ttcagcggcc acactacagc
     1681 cgttatctct gacatagccg cggatagtaa tcgtgctatc cgcagccaaa acgcgactta
     1741 ccgccagcca caaaaaagcg cacagaagat aaaaaggttt gtttctcatc acgccccctt
     1801 aacgacattc agctgatagc tgggttaata actgctgctg actctctggt ggcagttgat
     1861 aattggcgat acagtgagca ttttcctctt ctccccattt cacctgaact tttcccgcta
     1921 aaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta ctctggctac
     1981 tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgggtt agcgtcatga
     2041 gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca cgagtgggaa
     2101 caacgttagc gaccgcgtta tctaaatcga cgttatcagc cagggtattg gtatccagcg
     2161 ccactctgtt ttcccgatat tcggtggcat aaggcagcac ggcataaccg cgccagtcgg
     2221 tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct ttaacaagta
     2281 ccaccgtatc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc agtaccccac
     2341 cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg atattggcat
     2401 tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt acttccgcta ttaccatcgc
     2461 ctcccccggc atagccggtt tgcacgctat agctgaggtt gttgtcttcc agcaacgtac
     2521 cgtatacacc agccagattg gtcatccgac cgttgagatc gtgtgacacg ctgtagctgg
     2581 cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg aaaggaatat
     2641 tgacgttaag cgctaacatc tgatcccgcc ctttttgcca ggcgtttttc gtcaggctat
     2701 agctgagcgt ccagttgata tcttcgaacg cagtatttaa tccagcctag aattgctcat
     2761 cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt gttgatgtgc
     2821 gcccgagttg ctgagtaacg gtgagctgta atttcccgcg tttgttataa gcgaggttgt
     2881 aatagtcggt gaatttcggc ttaacctgaa taactccatc ctgtgtttcg atgttgtagc
     2941 cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc gaataacggt
     3001 aacccactaa ctgaatattc gtgcctgact cattgagcga tttgttatag agaaaacgca
     3061 ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca
     3121 cagacagagc gcccagtgcc cccatgtttt tcccgatacc gaaattaaaa gcacgataac
     3181 gatccgccag ttgcgttcca ccatatattg tccagccagc cggaaggccg tggagtaatg
     3241 tactttggaa aaagcggggt ttttcctgct gcgtatttcc actacggtat tctcctgccg
     3301 taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg
     3361 taaaaatctg cgtgctgccg tcagcctctt tgatcgttac ctgcaagtca ccactattac
     3421 ctgcggcata gatatcgttg atggtaaaag gccccggtgg caccgtacta ttataaatgt
     3481 catacccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg tggatcaccg
     3541 gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc
     3601 gaaagttaat accatcgaaa atatcgccct gggtataacc atcacccagc gtcagccggg
     3661 aacgtaacgg tattatgtct cgctcaagcc aggtattgat atgctgccat ttatttttgc
     3721 tacctgatga tctgtcgcta ctgttataac tccagctggt attgtcgcgt aaacaccacg
     3781 caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta cccccaatct
     3841 gattctgtac actatttccg ctgaaattat aattgagcaa tccggcatta ataccgggat
     3901 cccataactc aggaggaata taaccacgcg cgcgattact cataaatgcc tgagggatcg
     3961 tcaggttcag tcgctgctga ccaacatcca gatgcgcagt agcgtcatgg atcattgagg
     4021 ttaatggcac gcaggcatca tctgccagca gattcatacc ggagacagaa gccgcattca
     4081 gccccatact ggcaagttgc gcgcgtgtca ggcagggaac aatcccttgt tcactgtcgc
     4141 ccgtattaaa tgtgacatca cgcgttgcca tataaccatt attcaaatag atatcgacgc
     4201 gatacgtccc tggcggtaat tcttgcccat tttcaaaacg cgataaatcg gccacagcct
     4261 ggggatcatc cgctaaaaag cgcggattaa aatagagttc ggcagatgac aaaggtgcct
     4321 gtgcggcaaa agcacaggcg acaacgagcc ggacaaaaaa accagccaaa cgatgcttac
     4381 gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa attcagatat gacattattc
     4441 ctccggttta aaattacccg gcagtcaatt ctttttattc aggcgagaaa ttccccctgc
     4501 gttattccat tacgcccgtc attttggggg taagtgcgcc ataatcattt attgttcggt
     4561 aagtaatatt gcttcctgca tcagaaggca atttaaccgt gctttcgccc attggaggca
     4621 ccaatgcatt ttcaagaacc cgggttccgg cattcaactc tgttaccgtc agataatagg
     4681 gtgtcgggtt aatcagcgtc agagaattcg cgctacgacg aaatcttaat ttttctgcgg
     4741 cctgatcggg tggcaacgct aatttagccg ggcgatagta cagtttaatg cggctgataa
     4801 ttgcgaactg tagcgtattc tcagtcaatt ttgatttatc cattgacgga atcgctttaa
     4861 cattcatcca gaataaactt tcccggtcct gtggcaattg gttatttgtt gcatcaagaa
     4921 tacgcaaggt attctctttt tttcccttca tcgcaaacag aggaggcgtc acgataaaac
     4981 gaccatcctt tacaccatcg gcattttcca tccatgattg aattaaatag gtactatttt
     5041 catcattatt tgtcacggca agttgcactt gtttttgccc tgccggataa attacgcgag
     5101 tcgcacctaa ggccactccc gcttcagcgc gtccggcaac catcattgcc atgaacatca
     5161 ggatacctgc cagcaagcag aatgttattt cctgcgattt ccttacattg gcgtttttat
     5221 tactcactgt cctgttcctg ttatcacatt atctgctgaa caattactga taggttaaag
     5281 agaaccaggc ctgggcattg gcgatgccgc cagtaaccca acgcccggta gcacgatatt
     5341 tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg tttccagttt actggaggac
     5401 gattaatcgg tacgaggttt ccttcatcat caaacaacgc tacgccaata ttggtggcta
     5461 tccctggccc ctctcccacg gaaagcacat ccggattttt accatccgcg acaccgtgaa
     5521 acgccacacc cacacgttca ctcaccaccg tgctacattc ccgtaaatga ataacaaaag
     5581 gcaccggtgc gctatcttcc cctaccgcat gaaaccggtt actgctgatt tgccccatat
     5641 tgaccgtcat ttgtttatca ccggcttcaa tccggcaagt ttccgcaata atgacgccct
     5701 gaaattgcat atttccgccg ggcagcgtag tattccattt atttccggcc agagcaaaca
     5761 ttggcagcaa cgacgctaat agaaataatc tcttcctttt catcgttatt tttatcgcac
     5821 aagggtgggc atccctgccc gtaatgacgt ccctgaacct gggtaggtta ttgatactga
     5881 accttgaagg tcgcatccgc attagctgcg ccaggggttg cgacaccggt tgcataataa
     5941 cgcgcctgga acggaatggt gttagtaccg ttattcaggg ttgtttgctc actgaatgtc
     6001 gcaccatcca gcgtcagcgc agcacccgtt ctgtccagga tctgtacacc aacgtttgtt
     6061 gcgctacccg cagctgaact ctgcagagcc agaacgttgg tatgacccgc atcaatcgcc
     6121 gtacctaaaa aggcaactgc ggctttagat gcaacattgg tatcgcaatc attcagctga
     6181 atgttaaaac cgacagcaga actggttgct ccttcctgtg ccagcgatgc ggtacgaacc
     6241 tgtcctaact gaacgatttg atcaacagag cctgcatcaa ctgcgcaagc ggcgttaaca
     6301 acttcccctt taaagtgaac ggtcccacca ttaaccgtcg tggcagcggc cagagccgct
     6361 gtagaactga gggacagagc cgacagaaca acgattgcca gagttttaat tttcatgctg
     6421 ctttccttta aaaaaactat ttctaaatcg acatgggcag tcgttctgta cactttgttt
     6481 tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga aacatttggg
     6541 gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta tttgttggct
     6601 taatattcta ttgttatctt tatttataga tttttatatt gcatgaggtg gtttttggag
     6661 agaagaatga ggaagatgcg tcgagccaca gaaacgttag ctttacatat agcggaggtg
     6721 atgtgaaatt aatttacaag agaaataatt tacatatcaa acagttagat gatttttgtc
     6781 gttttttaaa atttttatgc ttgagaaaaa atacgtaact tatttatgat atggacaatt
     6841 tggccccaat tgtcttgtat ttatttttta actttattat caataagtta attcaaacct
     6901 cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag
     6961 cagcattact ggcggtataa cgtacagtat ggcgaatatt tcgatgcccg agataatcct
     7021 gaattaaacg agtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca
     7081 tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcacga ataatgcgat
     7141 aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatggcg tcggtccggt
     7201 cagcgccttt ccagttagca cgttccaggg tccagcgttc cacagcttca cgctcatcaa
     7261 aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt
     7321 cattaaggtc aagatcctga taatgcagat caagcagttc actaatacgc atcccatgcc
     7381 gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct
     7441 gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac
     7501 tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc
     7561 ccaatagata aaaagataac cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa
     7621 tatcatacaa aaataaaata tcactgatgt gacaacatct attttcattg atttgattca
     7681 atataacaat agattgtagt agattagctt ccagttgctt aaaaaataaa gaaaaaagtg
     7741 cgaaaacagg agaatttaag tgatgtgtaa atttatgaat gattttgttt atttattttg
     7801 aagaacataa accattgtat tttgtatgga tatggttaac ttaatgctta ttatgataat
     7861 taagaattat acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt
     7921 aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcgt
     7981 gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg
     8041 tataccagac agtatgacga atattgcgat gcccaagata atcctggata agtcgcgtat
     8101 ctattcccat attcgccaaa gcaaaaccac acgaatggcg taacatgtgc ggatgaatct
     8161 ccagtgacaa cccggcatta ccaccggaag tcgagataat atggtaaaac tgttgccgag
     8221 aaagcggatt ccccttacgc gataaaaata cccactcgct ctcagcatgc gggtacgaag
     8281 tacggatact caaccagttt tttaaagcct gaacttcttt attcaatagc gggggcgttg
     8341 ttgaaaagcc tttttttaat cgatggatat atatacactt tgccttaaga tcaatatccg
     8401 aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaagtca
     8461 gacaataatt acgtgctgca tgaggcccgg tatttgctgc tttaaggagt gattcgattt
     8521 cactatgggt caggaagttc ctttttttgt tatcagcctt attcttcatc gtttttccct
     8581 tataattaca gacgcgcact agctgtgctg ggttagcaat aatccaacat ttttatcctg
     8641 attatgttta taaaagcgaa cgtttgctta ataactaacg taagtggacc agttcttctg
     8701 agtgaactta aatggggtag caacagttaa ttataataac gttgccatac aaacgctatg
     8761 ctcttgcatg ctatgtacct ttatatattt atcaatcgga tacgaatcta ataatacccc
     8821 tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt aacaagtaaa
     8881 atcagcataa aaaattcagc aaaatttaat attcgttcaa ctagccgtct cacatatgac
     8941 attattagtc aactccattt gccacaaaat ggctatttca tggaacgcag taatttctta
     9001 gtggctatta tcatgctaat tcttaggtcc ctagcgatta ttcctggctg attcgtcagc
     9061 ttacgccatg agattgttac attccttttt atcctggaga tatgggggaa cgcaggagat
     9121 gattttttca tctacattcg ttggtaaata tagctttggt cactacgact taaaccttcc
     9181 tcaatcacag ccattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     9241 aacaccaaca tcaacaagcc tctccagatc gacttcagaa gtgaccagtt acaagccaca
     9301 aaccaggaaa catatttcat gatgagaatt atgctcagcc tgatggcggg taaacactga
     9361 ttttcaccaa gctcactgag ttagcttgca aagcttccct tgtttagact ccaaataaat
     9421 tatttataaa acaaacattt aaacaaagat ctttagtttt tgaagaccac cgcaagtgtt
     9481 cgtctggctt cacatggcat cttcctctta gaaaaagatc gacatatttt gtgacacgaa
     9541 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     9601 aggtgcacca agatagtcaa tgagacaggg catctcgcaa tctatggcaa acatcacttc
     9661 agttctttct catcgggtga tgaaaacgca cttcagtctg aaaggaatat gaaaatgaga
     9721 tcaacagaca ctctatttta tgactctggg taaaatggat tgagtaagtg atatagctta
     9781 cgaaaattca aatcaattaa acatcagaag agattttata ctcaggtatt taatctggat
     9841 ctctgtttat ttaaataatg tgaaaagaga tttttcacag gagaccttat acaaaaaaat
     9901 ataaaataca gctaccggtt gccaaagaca ctataagcct ggcaaaaaat attacacaac
     9961 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
    10021 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
    10081 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
    10141 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
    10201 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
    10261 ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
    10321 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
    10381 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
    10441 agacttatcc agtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
    10501 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga
    10561 ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca
    10621 tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc agggtgttta
    10681 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
    10741 gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc atgatggctt
    10801 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
    10861 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca
    10921 agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc cctggtggac
    10981 caagagatca agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg
    11041 gcaaaaacag agagggcttg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
    11101 ccaatagctg ggttaaattg atgtcgcacg caccgatggg catggcgggt catgtgactt
    11161 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
    11221 attttgaaga tctcaacgag gctggaaaag attcaaccgc tgtagataaa atcaatgcct
    11281 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
    11341 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
    11401 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa cccggattgc
    11461 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
    11521 ctcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
    11581 ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag cacggaaaga
    11641 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
    11701 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
    11761 ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
    11821 cagttttgat ctctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
    11881 cagtaaaggg gccctgctat caatttatca tttcaattac gcgaaaatta tatggacgca
    11941 ataatatcgc ccgattatta ctatgttctt accattgctg gtcagtctaa tgctatggcg
    12001 tatggcgaag gactgccatt accggccagg gaagatgcgc ctcatcccag aattaaacaa
    12061 ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa cgacattatt
    12121 ccactaactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
    12181 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
    12241 ctgtccttta ttcctgataa cgcaggggtt ctcatcgttc cgtgttgccg tggcggatcg
    12301 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
    12361 tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac gcgagccgca
    12421 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
    12481 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
    12541 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
    12601 tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga agctatttat
    12661 ggcaattatc aaaataatat tttagctaat attattttcg tcgacttcca gcaacaaggt
    12721 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaatcat gggatattac
    12781 ggttcagcgt accgctcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
    12841 gcggcagccc gtcgggggat tatttctgac aggtttgtag aagcaatttt gcagttttgg
    12901 cgcggaaagt gagcgttcat taatttgatt tattcgcatc catcaacgct tttaactcct
    12961 ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg gcaattaggg cacacaagca
    13021 gcaaaaaaac aaatgagatc aaggtaatat atcttcactt gcaggcttga acagcagaaa
    13081 atttgcgagc atgatcgcaa ttatttcact gctagggata tcaatggctt ccagtaaatc
    13141 gctacagcag gcaattgcca atataaaaat ctggcataag ggtgaacagc gcgcaccgca
    13201 taaaccattg ttattgctat acgtattagc gggatacctg aatggacatc cacgcctttt
    13261 cgattatgga tcggaaatct acgaacctct gcacagttta ctggaacgtt ttgggccaca
    13321 gcgttcacag taccgacctg atatgccgtt ctggcgatta caaggcgacg gattctggca
    13381 attacacaat gccgagcttt gctcaacagc agggagcagc cgacaaccgc cagtgaagga
    13441 actgaacgaa taccacgttg caggcggttt cgatgaacag cactacgccc tggtaaccgg
    13501 caataaaaaa ctcatcaata ctctagcgca acagatactc gaagcgcact tcaccgagag
    13561 tattcaggaa gaaattgctg acgagctggg ttttgatctc cagcaaatcc gtaaacagcg
    13621 cgacccgtta tttcgcaaaa atgtattacg cgcttataac taccagtgcg cgatatgtgg
    13681 ctttaatatg cgccacgacg acaccaccgt cgcgctggaa gcggcgcata taaaatggaa
    13741 acagcacggc ggtccttgtg aaattcctaa tggcctggcg ctttgcgcaa ttcaccataa
    13801 agcctttgat aaaggatcga ttggcctgga cgaaaatatg cgggtactgg tatcagatgc
    13861 agtaaacggc ggagggagcg ttgagagatt attctgggat tttgacggga aaattattgc
    13921 gttgccgcag gtgcgtaaaa attaccctta tgaggggttt gtggagtggc atcggaatga
    13981 ggtttttagg gggtgaggtt gataactatc gcagtatgtt caatattgct gacataatca
    14041 gtgcaggatc ttgccgctag tttacaactc gccttcgata gcggcgcaaa acgagttctg
    14101 ttgccgatat cctgggctat ggatattcca acagttccgg cagagttgtt taccaagttt
    14161 caggtaagtt tttactcaga accggttgat gcggtttata aggcgctgga tgtgaattaa
    14221 cacattcctc agcttattta caaaaggatt attcccattt gatataatat cagtgccaat
    14281 ttactttttt caatggaata tatctcggct aaataacaat aaaacttatc
//
LOCUS       DAJVTY010000104        14212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVTY010000104 DAJVTY010000000
VERSION     DAJVTY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.104"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OQQ66_002750"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OQQ66_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1905620.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OQQ66_002751"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OQQ66_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1905621.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OQQ66_002752"
     CDS             complement(2788..5901)
                     /locus_tag="OQQ66_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1905622.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OQQ66_002753"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OQQ66_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS1905623.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OQQ66_002754"
     CDS             7143..7421
                     /locus_tag="OQQ66_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905624.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OQQ66_002755"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OQQ66_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1905625.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OQQ66_002756"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OQQ66_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1905626.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OQQ66_002757"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OQQ66_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1905627.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OQQ66_002758"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OQQ66_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1905628.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OQQ66_002759"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OQQ66_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OQQ66_002760"
     CDS             complement(11102..11632)
                     /locus_tag="OQQ66_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1905629.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OQQ66_002761"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OQQ66_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1905630.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13826)
                     /locus_tag="OQQ66_002762"
                     /pseudo
     CDS             complement(12709..13826)
                     /locus_tag="OQQ66_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3945 a         3018 c         3153 g         4096 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac atcatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagaacga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata
    13321 aaattagtat ctgaaaacat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg
    13381 ctaattttag ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat
    13441 tcagcacttt tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc
    13501 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
    13561 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
    13621 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat
    13681 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
    13741 gttttaacat tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt
    13801 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag
    13861 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
    13921 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
    13981 ttgtgtataa aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
    14041 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
    14101 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
    14161 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gc
//
LOCUS       DAJVTY010000105        14188 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVTY010000105 DAJVTY010000000
VERSION     DAJVTY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.105"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(92..388)
                     /locus_tag="OQQ66_002763"
     CDS             complement(92..388)
                     /locus_tag="OQQ66_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905631.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            672..2303
                     /gene="oppA"
                     /locus_tag="OQQ66_002764"
     CDS             672..2303
                     /gene="oppA"
                     /locus_tag="OQQ66_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1905632.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2389..3309
                     /gene="oppB"
                     /locus_tag="OQQ66_002765"
     CDS             2389..3309
                     /gene="oppB"
                     /locus_tag="OQQ66_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1905633.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3324..4232
                     /gene="oppC"
                     /locus_tag="OQQ66_002766"
     CDS             3324..4232
                     /gene="oppC"
                     /locus_tag="OQQ66_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1905634.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4244..5257
                     /gene="oppD"
                     /locus_tag="OQQ66_002767"
     CDS             4244..5257
                     /gene="oppD"
                     /locus_tag="OQQ66_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1905635.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            5254..6258
                     /gene="oppF"
                     /locus_tag="OQQ66_002768"
     CDS             5254..6258
                     /gene="oppF"
                     /locus_tag="OQQ66_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1905636.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6311..6640)
                     /locus_tag="OQQ66_002769"
     CDS             complement(6311..6640)
                     /locus_tag="OQQ66_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1905637.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6675..8135)
                     /gene="cls"
                     /locus_tag="OQQ66_002770"
     CDS             complement(6675..8135)
                     /gene="cls"
                     /locus_tag="OQQ66_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1905638.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8278..8451
                     /locus_tag="OQQ66_002771"
     CDS             8278..8451
                     /locus_tag="OQQ66_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1905639.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8506..9759)
                     /gene="kch"
                     /locus_tag="OQQ66_002772"
     CDS             complement(8506..9759)
                     /gene="kch"
                     /locus_tag="OQQ66_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1905640.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10059..10355)
                     /locus_tag="OQQ66_002773"
     CDS             complement(10059..10355)
                     /locus_tag="OQQ66_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1905641.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10570..11298
                     /gene="tonB"
                     /locus_tag="OQQ66_002774"
     CDS             10570..11298
                     /gene="tonB"
                     /locus_tag="OQQ66_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1905642.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="OQQ66_002775"
     CDS             complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="OQQ66_002775"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1905643.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11841..12380)
                     /locus_tag="OQQ66_002776"
     CDS             complement(11841..12380)
                     /locus_tag="OQQ66_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1905644.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12410..13153)
                     /locus_tag="OQQ66_002777"
     CDS             complement(12410..13153)
                     /locus_tag="OQQ66_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1905645.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13181..13270)
                     /locus_tag="OQQ66_002778"
                     /pseudo
     CDS             complement(13181..13270)
                     /locus_tag="OQQ66_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13510..14148
                     /gene="ompW"
                     /locus_tag="OQQ66_002779"
     CDS             13510..14148
                     /gene="ompW"
                     /locus_tag="OQQ66_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1905646.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         3823 a         3315 c         3470 g         3580 t
ORIGIN      
        1 cgcagttatc aaaagcaaaa ggaataggta aaaatattct tctcaaatta cagttagtta
       61 taaggatttt cttaactgct tctcctcacc atcatgttat tttcgccaca tcataatcct
      121 gggcttgctg aagaataatt gaaatgatat tagtaattcc actgcctttg gtagaggaaa
      181 gtgctaaata ataatcaatt gttaaattat tgtgcatttc aatactgaaa ctgtaatcag
      241 aaaagataga catgcttagc caatctctat ttgattgaat tgaaagatgt ttgttaaggc
      301 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
      361 aaaatgtatt ggcgagtaag aaccgcattt agtactttcc gggcaaccgc cagacgattc
      421 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
      481 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
      541 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
      601 tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag
      661 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
      721 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
      781 aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
      841 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
      901 atcttgacgg tcatccggca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
      961 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggtacgcca gtcacagcac
     1021 aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct ccgtatgcca
     1081 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
     1141 ttaccgatct cggtgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
     1201 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
     1261 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
     1321 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact
     1381 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
     1441 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
     1501 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
     1561 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
     1621 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
     1681 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccgg
     1741 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
     1801 ctggttatac cgcagacaaa ccgttgacaa tcaacctgtt gtataacacc tccgatctgc
     1861 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
     1921 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg
     1981 tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc
     2041 tttcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
     2101 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
     2161 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
     2221 tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga
     2281 atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt
     2341 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
     2401 attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
     2461 tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgccagaa
     2521 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
     2581 agctacctga aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat
     2641 tcggtcaatg acttggttgc atccagtttt cccgtttctg ccaaactggg agccgcagca
     2701 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
     2761 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
     2821 gtggttgcac cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
     2881 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgct
     2941 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaat
     3001 tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgtcacgca
     3061 ttaaaacctg ctctgttacc cgtgctctct tatatgggcc ccgcatttgt cggcattatt
     3121 accggttcta tggttatcga aaccatttat ggtttgccgg ggattggaca actgttcgtt
     3181 aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgct
     3241 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
     3301 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
     3361 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
     3421 ttattcataa ccgtgcggcg gttgccagtc tgatggtgct ggtgctgatc gcgttatttg
     3481 taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg gcgatgatgt
     3541 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
     3601 tgcttgtgcg cgttgcgatt ggtgggcgta tctcactcat ggtcggtgtt gctgcggcac
     3661 tggtggctgt ggtcgtcggg acactttatg gttcgctttc cggttatctg ggcggtaaag
     3721 tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg
     3781 tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgataggca
     3841 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
     3901 aagagtttat tgaggcggca caagttggcg gtgtatcgac atcgggcatt gttattcgcc
     3961 acattgtgcc gaatgtactc ggtgtggtgg tggtctacgc atcacttctg gtgcccagca
     4021 tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca
     4081 gctggggtgc attgctgagt gatggcgcga actcgatgga agtctctcca tggttattgt
     4141 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
     4201 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
     4261 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg
     4321 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct
     4381 acgtgccgga gaaacgctgg gtattgtagg tgagtctggt tcgggtaaat cgcaaactgc
     4441 atttgcgttg atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa
     4501 tgggcgtgaa atcctcaatt tgccagagca tgaactcaat aaactgcgcg ctgaacaaat
     4561 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgtg tcggtgagca
     4621 gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc
     4681 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc
     4741 gcacgagttt tctggcggca tgcgtcagcg agttatgatt gcgatggcat tgctatgtcg
     4801 acctaagctg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg tacaggcgca
     4861 gatcatgacg ctattaaatg aactgaaacg ggaatttaat accgccatca ttatgattac
     4921 ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg
     4981 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
     5041 cggtttgctc aacgcggtgc cgcgtctcga tgcggaaggt gaaacaatgt tgaccatccc
     5101 gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
     5161 gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ttggccgtct
     5221 gtgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
     5281 gtcctccttg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
     5341 ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcacgcttcg cctgtatgaa
     5401 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
     5461 atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa agagttgctg
     5521 ggcattaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
     5581 ccgttggcat cgctaaaccc gcgcatgacc atcggcgaga tcatcgctga accactgcgt
     5641 acttatcatc cgaaaatgtc acgtcaggaa gttcgcgagc gcgtgaaggc gatgatgctg
     5701 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag
     5761 tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgtgat
     5821 gagccggtgt cggcgctgga cgtgtcaatt caggcgcagg tggtcaacct gctccagcag
     5881 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
     5941 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
     6001 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
     6061 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg
     6121 tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg
     6181 gagtgcgcca aaacacgtcc tgtgctggaa gggagtttca gacacgccgt ttcttgcctg
     6241 aaagtcgatc cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgccga
     6301 aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
     6361 acagaatgcg ggcgaaaaca tcgttgattt caccatcctc actgtccgcc agaccaatca
     6421 ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc cagtcttcca
     6481 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
     6541 cagctgggtc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
     6601 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
     6661 ctgttggaga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
     6721 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
     6781 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc
     6841 tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg
     6901 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
     6961 ccagcagcaa gaagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
     7021 ttttttcgcg gaaggataat actgacatcc accccgcgct gtgccgccgt gcaaatcgca
     7081 tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc
     7141 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa
     7201 gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt
     7261 ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc
     7321 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
     7381 gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa
     7441 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
     7501 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
     7561 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
     7621 ccgcgtcgcg cagcggccat taatgattca gccacctggt ccgccatgcc gccgggctgc
     7681 cagatataaa acacaatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
     7741 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
     7801 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
     7861 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
     7921 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
     7981 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
     8041 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
     8101 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
     8161 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
     8221 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
     8281 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
     8341 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
     8401 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgtactgg
     8461 gcaccattgc ggtgcccaat aagtttactc ctccatcttt aaactctatt tttgcgccga
     8521 ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatgc catgaccgga
     8581 gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc
     8641 cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat
     8701 cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc
     8761 ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc
     8821 caggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt caccggggat
     8881 aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt
     8941 gctgataacc gttacgtttt gtccgcgttg attcagttgc agaatcgtat tgatggcgag
     9001 aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt
     9061 tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc
     9121 aaaaacggta ataccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac
     9181 agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt
     9241 catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga
     9301 gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact
     9361 gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat
     9421 actaaatttc aaccagggat aaaaatgcag ggtataaatt agcgctatca acaacaaaat
     9481 gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag
     9541 aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca actctgaaaa
     9601 tgctttgata tggaaaatat ctagcaaatt gaccgacata ccatagattg ttttaaaaat
     9661 aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg tcacccataa
     9721 atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat
     9781 tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt
     9841 aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagcgagc aactgtacct
     9901 caggaaaggt catttttcct gaatatgctc acatcatata aagaaataca gataaagtta
     9961 ttatctgctt gtggtggtga atgcactgac cggctataag gaaaggccaa acaagaacac
    10021 ggttgcaaaa accgtgccct taaatattga atctctattc agaacacttt cttaaatggt
    10081 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
    10141 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    10201 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    10261 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    10321 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    10381 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    10441 agaccatgca taaagtaagg ttaattacgg caaaaatgac attttcactg atcctgatcg
    10501 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgaggcttgg tttttctact
    10561 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    10621 ctttcggtct gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    10681 gttattgaac tacctgcgcc tgcgcagcca atttctgtca cgatggttgc gcctgctgat
    10741 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    10801 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    10861 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    10921 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcactggc acgcctgaca
    10981 tcaagtacag caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    11041 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    11101 caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc
    11161 tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    11221 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    11281 accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta
    11341 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    11401 ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    11461 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacactac
    11521 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacgg tcactacgcg
    11581 accgtgggca atttctttcg ccagaatagc gccgccaata tccatttgtg acattaacca
    11641 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    11701 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    11761 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    11821 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    11881 ataccgctta acaatgtgaa gattaaggta agggcggtca gtccaaagac tttaaagttg
    11941 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    12001 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    12061 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct
    12121 cccgcaaaca gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag
    12181 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    12241 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    12301 gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    12361 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    12421 tcaacatata cagacggaac agatagatga gcaatacggc tgaaatcagg ttgctcaagg
    12481 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    12541 gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg
    12601 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca
    12661 gcatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg accactagca
    12721 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac tttggcaata
    12781 tcggtgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    12841 ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg
    12901 acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    12961 cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat
    13021 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    13081 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    13141 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    13201 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    13261 gcggaggtaa ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa
    13321 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cgttaaccag gattctcaat
    13381 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    13441 gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac
    13501 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct tctctctggc
    13561 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    13621 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    13681 actcaactgg gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    13741 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    13801 caccatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    13861 cgtccttacg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    13921 catggcaaag aggcagggct ttccgatctc agtctgaaag attcctgggg agctgccggg
    13981 caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc agtctggtac
    14041 atggatatcg ataccaccgc caattataag ctgggcggtg cacagcaaca tgatagcgta
    14101 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    14161 acgaccccgt aatatacggg gtcaataa
//
LOCUS       DAJVTY010000106        13938 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVTY010000106 DAJVTY010000000
VERSION     DAJVTY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13938
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.106"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..416)
                     /locus_tag="OQQ66_002780"
     CDS             complement(<1..416)
                     /locus_tag="OQQ66_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1905647.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAM"
     gene            complement(804..1550)
                     /gene="cutC"
                     /locus_tag="OQQ66_002781"
     CDS             complement(804..1550)
                     /gene="cutC"
                     /locus_tag="OQQ66_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1905648.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1564..2130)
                     /locus_tag="OQQ66_002782"
     CDS             complement(1564..2130)
                     /locus_tag="OQQ66_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1905649.1"
                     /translation="MANWQSIDELQDIASDLPRFTHVLDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2346..4079
                     /gene="argS"
                     /locus_tag="OQQ66_002783"
     CDS             2346..4079
                     /gene="argS"
                     /locus_tag="OQQ66_002783"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1905650.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWHKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4256..4744
                     /locus_tag="OQQ66_002784"
     CDS             4256..4744
                     /locus_tag="OQQ66_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1905651.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4864..5256)
                     /gene="flhe"
                     /locus_tag="OQQ66_002785"
     CDS             complement(4864..5256)
                     /gene="flhe"
                     /locus_tag="OQQ66_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCS1905652.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5256..7334)
                     /gene="flhA"
                     /locus_tag="OQQ66_002786"
     CDS             complement(5256..7334)
                     /gene="flhA"
                     /locus_tag="OQQ66_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCS1905653.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7327..8430)
                     /gene="flhB"
                     /locus_tag="OQQ66_002787"
                     /pseudo
     CDS             complement(7327..8430)
                     /gene="flhB"
                     /locus_tag="OQQ66_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8513..9157)
                     /gene="cheZ"
                     /locus_tag="OQQ66_002788"
     CDS             complement(8513..9157)
                     /gene="cheZ"
                     /locus_tag="OQQ66_002788"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1905654.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9168..9557)
                     /gene="cheY"
                     /locus_tag="OQQ66_002789"
     CDS             complement(9168..9557)
                     /gene="cheY"
                     /locus_tag="OQQ66_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1905655.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9572..10510)
                     /locus_tag="OQQ66_002790"
     CDS             complement(9572..10510)
                     /locus_tag="OQQ66_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCS1905656.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10513..11325)
                     /gene="cheR"
                     /locus_tag="OQQ66_002791"
     CDS             complement(10513..11325)
                     /gene="cheR"
                     /locus_tag="OQQ66_002791"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1905657.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11463..12029)
                     /gene="flhC"
                     /locus_tag="OQQ66_002792"
     CDS             complement(11463..12029)
                     /gene="flhC"
                     /locus_tag="OQQ66_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1905658.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLGRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12032..12382)
                     /gene="flhD"
                     /locus_tag="OQQ66_002793"
     CDS             complement(12032..12382)
                     /gene="flhD"
                     /locus_tag="OQQ66_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1905659.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13161..13589
                     /gene="uspC"
                     /locus_tag="OQQ66_002794"
     CDS             13161..13589
                     /gene="uspC"
                     /locus_tag="OQQ66_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1905660.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13596..>13938)
                     /locus_tag="OQQ66_002795"
     CDS             complement(13596..>13938)
                     /locus_tag="OQQ66_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCS1905661.1"
                     /translation="DGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYD
                     RDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQ
                     RDKVATFPKLA"
BASE COUNT         3589 a         3692 c         3256 g         3401 t
ORIGIN      
        1 tccatggcat caaaagaatg gcaactacgg cacgttgcag agtcagttgc ttttaattct
       61 ttccatactg tttcggccat ttcctggcga tgagtttcga acttatcgtc actgtctatt
      121 ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc aaataaataa
      181 tccatccctg actttggaat atggcaatct gcacattccg cacgtatccc tttctggttc
      241 gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg gcaggaaagg
      301 caaaatgctg tatccgacgt tttatgtaag gctttttgcg ccagcaataa cccgccgcca
      361 ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc tcgcatagat
      421 tcctctaaat aaattccaga acaccgaaat cattggataa ttaaatacac aacgaattaa
      481 ccgcttgcat aactcggcac aacactgcct gaaataatcg ataaagaata tgatttatta
      541 caatgtaatc attaattgct aaggaataac cctgttgtta tttagaatat atatttgtca
      601 gttttagcaa tttaaacccc ataattaatg tgaatatata aaatattttt agcacaacgc
      661 tatgttcact gtcacccgct caacagatca cggttatcgt acgtttttta tactgctcag
      721 ggataaaaaa aggcccctgt cgaaattgca ggggcctggt acgagcaagc atcatattgg
      781 gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt
      841 tcagcaaccg ccgccccgtc tacgacataa cgcgaatact cgtccgcgtg tgcatctgat
      901 gacatggaca atccttgatt acgataacgc atcggtgaag cttgccacgc tcccgcggag
      961 ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct
     1021 gctccggcca taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa
     1081 ccttgcagtg catctgattt ttgccctgat gtcagcaccc gggcgatgcc caattccgca
     1141 agattactga gtgtatttaa agggttagcg cacatatcga aggcgcgatg aaaagtcact
     1201 gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca
     1261 acatcgagca cgcccgtcac cagtccagga aaacctaatt cgcggactgt gcgcacatcc
     1321 tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcggata
     1381 atcggatgca cagggatcgt cacccactgg cgcacggatt tcagtacacc caacgacggc
     1441 gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc
     1501 gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat ttttactcct
     1561 taattacgcc gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat
     1621 agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat actcaccttt
     1681 tggcgaactg gttttcactg agatccccgg taggctgagt ccttcatcag aaagcaatgc
     1741 cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca
     1801 accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacaatgg aaaactgcca
     1861 gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat
     1921 attttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct cagcggtgac
     1981 gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag
     2041 acggcgggag agttcatcta atacgtgggt aaatcgcggt aaatccgatg caatatcctg
     2101 cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt
     2161 aatttaccct gttgccctgc gccaaccaac cgctgatttc acgccgcttc tgatgcaata
     2221 gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact
     2281 cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta
     2341 ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag
     2401 gcgcgcctgc ggattgcgaa ccgcaggttc gtcagtcagc aaaagttcag ttcggcgact
     2461 atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag
     2521 cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg
     2581 gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg
     2641 cgctggcgtc cgatcgtctc ggtgtttcta cgccagaaaa acagaccatt gtggttgact
     2701 actctgcgcc aaacgtggcg aaagagatgc atgtcggtca cctgcgctct accattattg
     2761 gtgacgcagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg
     2821 tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg
     2881 aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc
     2941 attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa
     3001 gcggtgacga atatttccgc gagatgtggc acaaactggt cgacatcacc atgacgcaga
     3061 accagatcac ctacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa
     3121 gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag
     3181 tagaaagcga aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac
     3241 cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg
     3301 cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact
     3361 cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac
     3421 cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac
     3481 cgttcaaaac ccgcgcgggt ggtacagtga aactggccga tctgctggat gaagccttgg
     3541 aacgtgcacg ccgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa
     3601 aactggctaa cgcggttggt attggagcgg tgaaatatgc ggatctctcc aaaaaccgca
     3661 ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat
     3721 acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaggcagaa attaacgaag
     3781 agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc
     3841 gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtga
     3901 tgtgtgctta cctgtacgat ctggctggtc tgttctctgg cttctacgag cactgcccga
     3961 tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag
     4021 cgaagacgct aaagctgggt ctggatacgc tgggtattga gacagtagag cgtatgtaat
     4081 cgatttttcg tgagagtaaa gcctgatcag ggttacctga tcaggctttt ttattgccat
     4141 ctaaatgtat tctgaaaatg gacatgccat tgttttctga ctgttggata agaggccaga
     4201 agcgtaatat ccggccccag ggaaacgata acggttgaat ttaaggaata ccgcagtgtt
     4261 taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt
     4321 tatagtaaac gaccatgaca tttcagccat caaagattgt tggcaaaaaa attcagatga
     4381 tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc
     4441 gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg
     4501 tgtaaaaaaa cctgatatcg aacaacttaa cttgcttaaa aaatcacagc gagcctggct
     4561 ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggacgttc aggatttaag
     4621 tgacccttat tctgaatcag aatggctctc atgtatgatc atacagacca atacgcgaac
     4681 tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg
     4741 ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat aattgcaaca
     4801 agacatcgag cctttttcac tgagttatta aacatactcg cgagcgcgta atttttttgt
     4861 ccttcagcga taattcacaa tcacttcatt acgttgtacc tttagcggtg gaattaaccg
     4921 cccaccgcct ggcacttccc agataaatcg caacggttct gctgccgcta taccggaaaa
     4981 ggccacggtg gttccgctct gcccctctaa ttcgacacaa cgagattgcg aacacaagcg
     5041 aacccgcagt cctgaaggtg tcgggccgat aagctgataa cgccacgcta ccagcgtcat
     5101 caatcctgaa gcaggttgtc gcgtagaaag aggcgcagac gacatcgact caccgcgatg
     5161 atttagcgta attcctacac tacttgcctg ccacatcccc tccccggcgg cttgcaccag
     5221 cagcggaaac aataatattg ctaataaggt tctcattatt tgccgccaat tgtcgccgtc
     5281 atgcggatat gtcggttatc agacagttcc agattcgaca ggaccactaa ctgcggcaag
     5341 ctgcggcgca ggaagcgaga caataatggt cgcagcgcgt ggttcaccaa caatactggc
     5401 ggcgcaccca gcatctcctg acgggatagc gcttcctgag tttgcgccag taaacgatcc
     5461 gccagccctg gctccagtcc tcccccgccc tgcagcgcct gtagtaacaa acgttccagc
     5521 ggtgtatcga ggccaataac atggacttca tctttgccag gaaaccactg ctgggtaatc
     5581 gcccgtccca acgccacgcg cacgacggcg gttaattcat gtggatcgct ttggatgggc
     5641 gcatgttccg ccagcgtttc gagaatggtg cgcatatcgc gaatcggtac tttttcatcg
     5701 aggagatttt gcagcacttt atgcagtgtg gtgagcgtga cgacgccagg aacgagatct
     5761 tccgtcagct ttggcatctc ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga
     5821 ccaaacagct ctgcggcatg ctggctaatg aggtggttaa gatgcgttgc taccaccgtg
     5881 ctggcctcaa ccactgtgta cccctgaatc tgcgcctgtt cttttagcgc actttcaatc
     5941 cagatagcat tcaggccaaa tgccggatcg acggtcgcct caccaggtaa cgtcccggca
     6001 gcggttccag ggttaatcgc caaccagcgc cccggataag catcaccact gccaatctcc
     6061 acgcctttca tcaaaatgcg atagcgggca ggttgcagat ccatattgtc gcgaatgtgc
     6121 accactggcg gcagaaatcc catctcctgg gcaaatttct tgcggatact gcgtatacgg
     6181 cccaacaact caccatcctg ctggaaatcg accatcggga tcagtcgata acccacttcc
     6241 attcccagag aatcttccag ttgtacatcg ttccacgtcg cttcgacaac ggtattattc
     6301 tctgccattt ttaccggttt gggttcggca ggcgcttttt gttcgcatcc gcgtatccac
     6361 caggccagcc ccagcaatcc ggcagtgaac agcaaaaata ccaggttcgg cattccaggc
     6421 accaagccga gtaaaccgag cacggcggcg cttaacaaca taacgcttgg gttactgaaa
     6481 agctgattca ccatctgctc gccaacatcc tgatcggtgc tgacacgcgt aacgatgacc
     6541 cccgcggcgg tagaaatcac cagcgccgga atttgtgcca ccagaccgtc gccaatggtc
     6601 aatagcgtat aactttccgc cgcgtgtccc atgctcatgc catgttgcag cacgccgacc
     6661 agcaacccgc cgacaacgtt aatgaccatg atgaggatcc cggcgatggc atcgccgcga
     6721 acaaacttac ttgccccgtc cattgagccg taaaaatcgg cttcctgagt cacttcggag
     6781 cggcgttttt tcgcctcatc ttcaccaatc aatccagcgt taaggtcggc gtcaatcgcc
     6841 atctgcttac ccggcatacc atcgagaaca aagcgcgcac ccacttctgc gatacgcccg
     6901 gcacctttgg taatgaccat aaagttgatg atcacgagaa tgacaaacac cacgatacct
     6961 atagcgaaat tgccaccaac gaggaagtga ccgaacgctt cgaccacctt ccctgccgcc
     7021 gccgcgccgg tatgcccttc catcaaaatg atacgggttg aagccacgtt aagtgccaga
     7081 cgcaacaacg tagtaaacaa cagaatggtc ggaaacgcag caaactcaag cgtgcgctgg
     7141 gtaaacatcg ccaccagcaa caccatgatc gacaaggcaa tattgaaggt aaacaacagg
     7201 tcgagtatga atgcgggcag tggcagcacc atcatcgaca agatcaacag gatcaaaatc
     7261 ggtccggcaa ggatctgcca ttgtgtcgat ttcaggtttg cgggcaggcg cagcatcgcg
     7321 gccagattac tcatgggtcg gtttctcgtt aataaaatcc agggcttccg gcaccggaag
     7381 atgagtaggt tgtacagggc gctgtccacc agccagacgc cagcgtttca gttgccagat
     7441 ccaggccagc acttccgcca ccgcggcgta cagttgaccc gggatttgtt gaccaatctc
     7501 cgcatgtcga tacagcgctc gcgccagcgg cggcgcttca agcgtcggga cgttattttc
     7561 agcgccaatt tcacgaatgc gcagcgcgac cagccctgca cctttagcga ccactttcgg
     7621 tgcgctcatt ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg ggttattgac
     7681 aatgacatcc gctttcggca catcggccat catccgacgc cgtgcggcag ctcgctgcat
     7741 ctgacggatc cgtcctttaa catgggggtc gccttcgctt tgtttgaact catcacgaat
     7801 atcctgccgc gacattcgca gctttttcag gtggctgaag atttggaaaa agacgtcaaa
     7861 tcccaccatt ggaatgacac caagcaccac cagcagtgcg catagcccta ccaaatccat
     7921 cgcattaccc atggcggtaa tcggagactc ggccagtaag cgcatcatct gcggccagtg
     7981 atgccagaga aaaaaccccg tcacgctgcc aaccaagatg gttttcaaaa ttgctttaag
     8041 caactccgcg ccagtctgtg ccgagaacat ccgtttaatg cccggtagcg ggttgagttt
     8101 ggaaaacttc ggctgcaagg attttccgct aaataccagc ccccccagca tgaccggaga
     8161 aatgagcgct accagcacca cgccgctaat cagcggtagc agcgccagca tggcttctct
     8221 gatcagcaga ataatctgcc cgaggatcag attcgggtct ttgataatac tgtgatcaaa
     8281 atgcagccca gcggagagca tgcccgacaa tcgacgggcc agcgacacac cgccgaacca
     8341 gataacacac acgcccacca gcaaaatcag cagtgaggtc agttcacggg aacgcgggat
     8401 ttagggcttt tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg
     8461 ccttatcaga ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc
     8521 caagactatc caacaaatcg tccacctgat cctgactggc taccacaccg gctttgctgg
     8581 tatcgacctg aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt
     8641 ccgggatgtt ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca
     8701 tcatccgctt aataacctgc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca
     8761 gcagttgcgc gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat
     8821 ctgttaccag ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag tcatcccaac
     8881 gttgggttaa cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa
     8941 cactgttcag cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat
     9001 cgcgcgcatc ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc
     9061 gcaaactgtc gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg
     9121 aatgctcgtc agcaggtttg attgatggtt gcatcatagt cgcatcctca catgtccagt
     9181 ttatcaaaga ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca
     9241 tagccactgg cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc
     9301 accattaaca ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc
     9361 aggccatcca tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc
     9421 aacttattga gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct
     9481 ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta
     9541 agttctttat ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa
     9601 tttttgccaa catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca
     9661 tattgatggc ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg
     9721 tccatgcccc cgcctgacgc atcgccaaca ttcccaccgg gccgtcgtga attttgattt
     9781 gataatttgc gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat
     9841 gccccggcaa gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat
     9901 taagtctgtc ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg
     9961 ccgggctgga aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg
    10021 aagcaccaat cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg
    10081 gtgctgacaa tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag
    10141 caatcatttc gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa
    10201 aatctattgc ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg
    10261 aagaaaccat cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc
    10321 cgtccatccg cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca
    10381 agtcacgcgc gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt
    10441 tgatgatttc tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga
    10501 ttttgctcat cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg
    10561 gcgctcaagg tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag
    10621 gagcggaaca aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac
    10681 gttacgacag aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt
    10741 cagcggggca aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg
    10801 cggtcccgtc cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc
    10861 ttcatggcga taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc
    10921 aaacactttc cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct
    10981 gtacggctct tcgccggttg aagccgccgc gctccatacg cgatactcgc cagaacggcg
    11041 acgtgcgtga tccgcgagca gagggaaatg atgtgcctca cggaaaaatg ccgtcagatt
    11101 cgtggtcagc gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa
    11161 gttcagataa tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt
    11221 gtaaaccatg tcgcgtttat gatcggccag gacgatcccg gctcgttgat agatcaattg
    11281 acttatccgc cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca
    11341 tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa cgcgcgattg
    11401 gggcaaaaaa aagcagcggt acgtcgttac cgctgctggc atgttgcgcc acaccgtatc
    11461 agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc
    11521 tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct gcaggcaaag
    11581 ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa
    11641 agttgcagta atccactttc aacaaaccgc cccaatgtcc aggcacgggt taatgccagc
    11701 agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc tttgatcacc
    11761 gcatcgacgc cattacacaa acctgttttc agtaaaaact gccatgcatt acagaacatc
    11821 gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct
    11881 ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc gcgacttaac
    11941 tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc aatatcccgc
    12001 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    12061 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12121 ggagatcgtt aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12181 ggaagtgaca aaccagttga ttggtttctg ccagcttaac catttgcgga agagtcagtg
    12241 ccgctaacgt tgtcgccatt tcttcattta tgccgagacg aaacatagcg gacgctttgt
    12301 cctgaacaat caaacgctgt gcaagtagta aatatgacaa gttgatgtca taaatgtgtt
    12361 tcagcaactc ggaggtatgc attattccca cccagaataa ccaactttat ttttatgcgg
    12421 tttcaccgca ccccgtgatg tcgccgggaa gccccggtaa aaaataatta gcattagaat
    12481 agtgcgataa gctgcaataa gcagaaccac ctttttggtt taatatgtcc ttacaaatag
    12541 aaatgggtct ttacacttat ctaagatttt tcctaaatcg acgcaactgt actcgtcact
    12601 acacgcacat acaacggagg ggggctgcga ttttcaataa tgtgtgatgc agatcacaca
    12661 aaacactcaa ttacttaaca taaatgtata agccatactt ttgttttggg tgtatttcaa
    12721 tctgttaaaa agtttttcgc tacgctagca agcaaaaatg aaactggaat aatcgaaatg
    12781 ggatgttgcg cacagttaaa ataactcacc gtaaataatc atctgctata aataatcact
    12841 ttcatgcaat accagataag ctatttttaa acagacactt accgcacaac aaactaatta
    12901 aatacaaaga aaggcaacaa tcaacttaat gttaatgaaa tgttttacat atattaacca
    12961 cttgtttaat gcggagaatc agtacactat tttcttataa ttacatttga aattatatga
    13021 tcaccgggag tgattgatta attgtcttta ctaataatca gactaatatt tacctgtttg
    13081 accgagttgg gattgcgtcg tttctccatt aggagtaaag ctttaatgtc acctgaagtt
    13141 cacagaataa ggaacaggtt atgagctata gcaatattct tgtcgctgtt gcggttacac
    13201 cggaaagtca gcaactgctg gcaaaagcgg tatctatcgc caggccagta aagggacaca
    13261 tcagtttaat tactctcgct tccgacccgg aaatgtacaa tcaattagcc gcgccgatgc
    13321 ttgaagattt acgcagcgtg atgcaagaag aaacgcaaag ctttcttgat aagttaattc
    13381 aggatgcggg gtatcccgtt gacaagacgt ttattgccta cggtgagtta agcgaacata
    13441 ttctggaagt atgtcacaaa catcatttcg atttggtgat ttgcggtaat cacaatcaca
    13501 gtttcttttc gcgagcatcc tgctcggcga aaagagtgat tgcctcaagt gaggtcgatg
    13561 tgctgttagt tccacttacg ggtgattaac cgctcctacg caagctttgg aaaggtagca
    13621 actttatcgc gctgctggct ttccgcgctt cgcggaacta tctgctttag gtcgctaatg
    13681 aagcactcct gccagtggtt aatatcgttt ttcacgataa cgtccagcat ttctgcatga
    13741 cgggaaatac gttccgccag cgacatagtc aatgcacgat ccagcgcagc tgcaacttcg
    13801 tcacgatcgt aggggttaac aattaacgcc gacgttaact cgtttgccgc tcccgcaaat
    13861 tgcgaaagaa caagaacgcc cgggttggct gggtcctgag cagcaacata ctcttttgct
    13921 accaggttca tcccgtca
//
LOCUS       DAJVTY010000107        13816 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVTY010000107 DAJVTY010000000
VERSION     DAJVTY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.107"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..565)
                     /locus_tag="OQQ66_002796"
     CDS             complement(<1..565)
                     /locus_tag="OQQ66_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1905662.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNS"
     gene            complement(568..1098)
                     /locus_tag="OQQ66_002797"
     CDS             complement(568..1098)
                     /locus_tag="OQQ66_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCS1905663.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1091..1987)
                     /locus_tag="OQQ66_002798"
     CDS             complement(1091..1987)
                     /locus_tag="OQQ66_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1905664.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(1991..2320)
                     /locus_tag="OQQ66_002799"
     CDS             complement(1991..2320)
                     /locus_tag="OQQ66_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCS1905665.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(2338..2904)
                     /locus_tag="OQQ66_002800"
     CDS             complement(2338..2904)
                     /locus_tag="OQQ66_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCS1905666.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(2916..3551)
                     /locus_tag="OQQ66_002801"
     CDS             complement(2916..3551)
                     /locus_tag="OQQ66_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCS1905667.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(3544..4011)
                     /locus_tag="OQQ66_002802"
     CDS             complement(3544..4011)
                     /locus_tag="OQQ66_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1905668.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4149..4556)
                     /locus_tag="OQQ66_002803"
     CDS             complement(4149..4556)
                     /locus_tag="OQQ66_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCS1905669.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHTERATASAVSPAYTADADHTGNASPLP"
     gene            complement(4553..4945)
                     /locus_tag="OQQ66_002804"
     CDS             complement(4553..4945)
                     /locus_tag="OQQ66_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCS1905670.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(4942..5265)
                     /locus_tag="OQQ66_002805"
     CDS             complement(4942..5265)
                     /locus_tag="OQQ66_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1905671.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5268..5468)
                     /locus_tag="OQQ66_002806"
     CDS             complement(5268..5468)
                     /locus_tag="OQQ66_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCS1905672.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(5468..5962)
                     /locus_tag="OQQ66_002807"
     CDS             complement(5468..5962)
                     /locus_tag="OQQ66_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCS1905673.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6130..6876)
                     /locus_tag="OQQ66_002808"
     CDS             complement(6130..6876)
                     /locus_tag="OQQ66_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1905674.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(6922..7794)
                     /locus_tag="OQQ66_002809"
     CDS             complement(6922..7794)
                     /locus_tag="OQQ66_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCS1905675.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(7791..7973)
                     /locus_tag="OQQ66_002810"
     CDS             complement(7791..7973)
                     /locus_tag="OQQ66_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCS1905676.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(7997..8833)
                     /locus_tag="OQQ66_002811"
     CDS             complement(7997..8833)
                     /locus_tag="OQQ66_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCS1905677.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEEITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            8988..10739
                     /locus_tag="OQQ66_002812"
     CDS             8988..10739
                     /locus_tag="OQQ66_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCS1905678.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10739..11785
                     /locus_tag="OQQ66_002813"
     CDS             10739..11785
                     /locus_tag="OQQ66_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1905679.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWRYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDIQPTS"
     gene            complement(12276..12866)
                     /locus_tag="OQQ66_002814"
     CDS             complement(12276..12866)
                     /locus_tag="OQQ66_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1905680.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(12930..13241)
                     /locus_tag="OQQ66_002815"
     CDS             complement(12930..13241)
                     /locus_tag="OQQ66_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS1905681.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13246..>13816)
                     /locus_tag="OQQ66_002816"
     CDS             complement(13246..>13816)
                     /locus_tag="OQQ66_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1905682.1"
                     /translation="YQNGEAFTIRNVQVMPESIPAGFKALADMSPFESLLIVDLGGTT
                     LDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIANTIIEHRHDEA
                     WLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVGGGAEIVAPAIR
                     EACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         2832 a         3852 c         3802 g         3330 t
ORIGIN      
        1 cgctgttagt ggcgctactg agttgaacaa agccttttgc ggtcagcgag gcgtccgggt
       61 gacgtcgtga ctgttcatgc tctttcagtt tgtcatccac gtaatccact gtggccatca
      121 ccatggtgtt gtccacggta agcgccacgg tggcagtgct ggatacggtc agaatggtgc
      181 gaaatgtttg tgcacgaccg gacccttcgg caacggctgg cttgtaactt tcggcagtat
      241 ttcccaccgc gattaaatcg ccgtgctcat caaatacacc aatttcccgg atccagaatc
      301 cgcccgtttc aggaggaata accagctccg caataatgcg gttctgatgt gttgcgtcca
      361 ggatgacgcg attaacagta tgtcgccaca cctcatgcac cagacgggtc tgcttactgt
      421 ctggtgtggg caacgtgccg ccaccgtcgc ccacggccat atgagtcagg cggacaggct
      481 taccatctgg cgcggctacc tgagctaatt tttttgcacc cgtatcggtg ataatggttt
      541 taaattttcg tgttgtggta ctcatgctta atcgcctgga taaatggtaa taacttcacc
      601 gtcataagtt gccgccgctg cgaaaatatc ccccggaatt tcctgaatga tattcagccc
      661 tgtcatgtgg cggctgaccg ggcgggcatc agcaatcaac cgctccattt ccagatacat
      721 ttcctccgtc acgccactgt ccagcgtgcc gacttcaacg gtaaatgttc ccggttctcc
      781 gccgaactcc caccactcag acacgcgaat gaggtatccc agcggctcaa tggcccggcg
      841 cagtgcgctg atggtccctt tgtgtcggtg tatcagccat gcatcacgaa taacctgtcg
      901 ctttgtctct tccggccagt tgcgatccca gcggtcaacg gaaaacgccc aggcgagata
      961 aggcagcaga tgcaccgggc aggtgtccgg cgaccacagc gtgttgaggt ctaccggaat
     1021 gtctgtaatg cgtgttccga cggcttcggc acaacgcatg aaattgctgg ctgatggtgg
     1081 taacaacgaa ttactcattg cgtccacctt cgctgatggt gaatgactca cagcgcgccg
     1141 cctgtatgtc gctgatggcc atattctgtg tgggttcgat tatctccacg cgttgcacac
     1201 cgtgcacatg cagtgcggca gcaatggcgg acaacgccac gtcctgaccg ataagcccct
     1261 gctcagccag ccacttcctg aacgacgatt cagccgcggc cagaataggt tcggattccg
     1321 ggccggggta aaagtacagt tttgcattca gccgccatgt cacgattctg gcactctgta
     1381 cggtcaggcg gtcggccacc gggcgggtat cctctgcatt cagaacggcg cgaacggtat
     1441 taagcaacgc ctccgttgct gtgccgtcgc cctcagtgga caggatggaa accgtcacgt
     1501 tggccggaga cgggctgatg gcccgcgcat cgcgtaccag accgctggcg ctgcgtgcaa
     1561 aatactcgta tgcacctgac gggccagcaa cactcagacc gtcatatgcc cgttgcgccc
     1621 gtagtctcag cgaggtgtca ctttccatca ctgcatcggt ggtatccgtt gccggagtga
     1681 taaccaggcg ctttgtgttc atattgcccg cgaggttgtc caggtctgtc ccggcgctgt
     1741 ggcttagcat gcaggcgcgt gcaccctcat tgacccgctg gcgtaacagc atttcacgaa
     1801 acgacatggt ttgagcgata acgttaaggg gttccgattc cagctccagc gcggcggaaa
     1861 cggcttcacg ctgttcggcg ggataggacg caatcatcat ggcctttgtg tcagccagaa
     1921 ttgcctcaaa gtcaggctcc gcgatgatgg cgggttccgg taactgtgaa aggtcaacgg
     1981 caggcatgat ttactccctc agcgtgatgg ttaattcaac attctgcatg gtctgcatga
     2041 cagtgcccga cagcgtcacc ccggcgcggc ctcctgcctt ccagacaacg tcgatagcgt
     2101 ccagggcaat gcggggttcc catcgtgtca gcgcaatcac ggcagcactc atgcattgca
     2161 gacgcgtggt gttattcatg ggttcgtcaa tcaaatcagg cacaaggctg ccatattccc
     2221 gtcgcataac ccggcttgcc agcggggtgg tcaggatgtc cctgactgac tgtttcaggt
     2281 gctccatatc gttcaggttt cccgttccgt ccgggttcat tcctgtgtag cgggttgtca
     2341 ctgcgggcct cctgtcgaat cgctgccacc tttaacgcca ccgtgtttat gcgtatgcac
     2401 tgtgatgccg tttgaggtga aattgccgcc gctgtgcgtg atattgccgc tcatctttcc
     2461 cccttttgtg acgtcaagcg tcgccgttct cagaaggtct gtgcattcca cgacgggcgt
     2521 atccagtgtc acgctgacgg atgcctgcag ggtggctgtt ttcatgccgc tggcgctcag
     2581 tgcgcctgcg tccgcgtcgt agcggaacac cgcgccatcc ggcgcgctga ccacgatttc
     2641 tttcaggctt ttgccggggg ccggaatggc atcactccac aggctgccaa ttatcatggc
     2701 ggtttccggg ttgccgccaa tgcaggcaat taccacctgt tcgcctggtg atggcggcag
     2761 ccacacattg aaggctcccg cgcgagaggt gttccagcgc agccaacctg tttccagttc
     2821 gccgctgcga acgcgcacgc gccaggactt ctcatcaact tcagagatga tcccggtgcg
     2881 gatgatattg ctcagtagcc gcatgagttc tgcgctcacc gtacagcctc cgcaatccgg
     2941 cccagcaccg tgttataaat caggcgctca tctgcctggc tgatacccag cagctcacgt
     3001 accgggtaat cggtgaaaat gcccggcgca acctgatcgc gctcaccgaa ctgatgaacg
     3061 cgtgcaatac gtgcggccac gccgctgtaa cccaccgtca caccggaagc atctgcacgg
     3121 gctttcaggt agcgggcggt gcgcagtttt acgaacatgg ggacgcgctt tgtgctgtcc
     3181 tggttgatgc gccgggtgcg tatttccaga aagcggtcga tgtcatcccg gtaaaacgtg
     3241 cggatgctgt ttttatcctc atcccacccg gtgatggttc gcccgtattt ccccgtgtcg
     3301 tgatgccagt ttttcagcgt gcgtgcttcg ttattccaga taaagcgaat gcgctcctgt
     3361 atccgggtta cgtggcgtct gcgtggtgtc catgcggtcc cgtccggcgc tttctgtggc
     3421 cggatacgtg cctgctgggc gcggcgtaaa tcctgtgcca gctttctggc gatgttattg
     3481 atggcctgct gattcaggct gtcgcggatg gcctcaaagg tttcatccac gcgagtgaat
     3541 gccttatcca tcgctttcac cccacgtcac atcctggaat acatgcgacc agtcgccttc
     3601 ggaagatggc aggcggggtt ttggctccag caggtgttct gcctgcggtg tgccctgact
     3661 gctgcgcgtg atgcgaacgc gttcccgcag ggggagcgta aacaggagat cggcgctgtc
     3721 atcgtcattg ataacggcgg aaaatttgat gtcctgatta cgctcagggt tgagcaacaa
     3781 ctgtggctga ttttcggata accacgccag cagcggcagc gtgaggtcgt ccagctcccc
     3841 ggcgtaatcc atgacaaaca tcaccatctg atagcggtaa acaaacgatg gggtttctcc
     3901 ggtcgtttca atgttgccgc tctccacgaa aatggtgaat ttttccgggt tagcctgaca
     3961 ccatcggcat gaacgggtca tggcttcacg cagggaatca gttttcagca tggttgttat
     4021 cctcgttgtt cagtcgttgc agcctgcgct gttccagtaa ttcaatggcc cgtttatccg
     4081 cgttacaggt ttccagtgca tccagaaggc ggtcgcccca tataccgaga tttccccatg
     4141 tgggagtgtc agggaagggg ggaggcgtta ccggtatggt cagcgtctgc ggtataagcc
     4201 ggactgacgg cgctggcagt ggcgcgttct gtgtgcctgc gcagcctgtc agtaaaacga
     4261 gcgtcaggca aagcgtgggc gcattcatct tttgcaatat cgttgcgtag ctgttcacgt
     4321 cttacctctc cgtcctgatt gcgttgctga ttttccacgc ggagttgcgc cagcacctgc
     4381 tgcatatcct gtaccccggc gctgatgata ttcagggtgt cggcggtact tttcagggtg
     4441 ctggcctgcg cttcgtttct ggcgttctcc cggcccagcg accacgacag acgcatggat
     4501 gttccccatc cggcagtcag aaggaaagcg acgccaagcg tgggccagag cttcatgccg
     4561 gataggctcc gtgtggtaac tgaaaatgcg gtccgtcttt cagggtcttc cagtcgccgc
     4621 cccattccac cggaatattc agttcccggc tggcctgtct gaatgctgct gcgatttttt
     4681 cgtacagcgg ccattcccat gacatctggc tgccgatata agccacaaca tccacggcat
     4741 gccccgtaag gtggcggctg ttcatggtct ggctcttacc cgtggccacc agttgcttct
     4801 ggcggtaacg gctgcgcaac ccttcggtga taccaaaatc cacttccgag atttccagtg
     4861 cccgtcgggt cactttcacc agatcaggat ttacaccctg caaattcttt tcgctccggc
     4921 tgctgaattt aaatgtgttg ctcattcgtc cttctccttc accctgcgat taaaggccgc
     4981 aataaccttg tcgcgtgctt tctctgcacc cataaaaccg attgatgcgc cgataaacgt
     5041 cacggcatct tcaggaaacc cgaagaagcg caacgacccg gccacggcca tggcaagaac
     5101 gccgcacgcc agcgatcccg ttacggtctg aaccagtgtt cgtccgtcat aaaggctcat
     5161 cagcgcggaa atgctgaccg ccgcgcctac tgcatacacc gttggcaggt ggtcaaagag
     5221 ccacgcaata acctgctctg tgatccctgt ttgaatggtg ctcactgcta ctccccccac
     5281 aactgaatca tttctcgttt cttcttctcc ggctccggca tctccacttc ctgcccggcg
     5341 tccagaaata cctgctgaca gagtccgggg ttggcatcca gcaccttttc ggtgacgccc
     5401 tgcgtcgtgc cgtagtaccg gaaacagagc gaatccacgg tgtcgccttc cagtgccttc
     5461 actttcatca gcacaactcc gcaaagattc gcgggcggca cagaatgtca gagatggccc
     5521 agctcacatc acgccacaaa tccgatgtct gtatatccag agcgtctgcc cggcggtcgc
     5581 ccttgtccgt agtgtctgca tcacgataac gctccagaat cagggcgcgc gtggcggtat
     5641 aaacagcatt gcgccagtgc cagagattga cgctttctcc gttaattacg ggtgccggaa
     5701 catcggccag cgtctgatgg ccagctgcct gctgttcctg ctgccatgct tccagctcgc
     5761 gggtaacgtg tgccacagcc ccggtggcag tatgcagcag gcgggaggtg gtcacgcgcc
     5821 ccggcagtcg taccgccaga cgcagctcgc gcagcacaat atccggccag aatgcacccg
     5881 ctgaaatgcg ggtatcgcca tcatcggtat cggtgatgtc gtcctctgcg ggtccgggtt
     5941 cagttctggc aactatagtc atggggttca ctcctgaaaa aaaatcgggc ggtgggtgcg
     6001 cggtgtaaac ggtcacggag tcaaactgga acaccgcgca cgccgcccgc tgacggggtc
     6061 agtcgttaac cgcgcttcgc cttctgcgtc gcggtggttt ttcgtgttgc aggcttccgc
     6121 gttgtctttt tacttttgct gctttttgct gctttcgtcc tgcgcctgct gtgcgctggc
     6181 gtcttctggt tcggctgcgg aatcggcttt tttcagggcg cgggaaaggg ttgcaatctc
     6241 gcgtttcaca cctgcgttcg ggttcaggtg cattgcttcg cgcagcagct tcagtgacag
     6301 ggccatgctg tccgcatcac tcaggccacg gcgggcaaag gcgcacgctt tgcataattt
     6361 ggcgcgcact tcgtccggca tgtcctggtc ggtgacaatc tcccggaggg tgtccagtgg
     6421 ttcgataaag gcggacaaat ccgcgtcggc atccgtcccg gcctgcgtca gtaccggatt
     6481 acagatttct tcggtcagta ccgtggcagc agtacggcca aagttatccg gcatgatgag
     6541 gttgtgacgg actacatacg cgccgatacg cagcgcaagc ggaagatcgc cacagtcaat
     6601 cgcccacacc atcagcgtgg caatcacttc atcctgctgc ccgccgtcag cctccagcgt
     6661 tccctcaatc cagccggaaa aatccggcaa caactctttt ttgatggcgg ctttcgcgct
     6721 tctggcctgt acgcccttaa gccgggcctg tgccagacgc agacgataca gcacctcttc
     6781 atgcgcggta cgcgcggcgt ggtccacgcc ttcattcgcc cggcctgcgc gctgtgccat
     6841 cacgttctgc cagtgttgtt gtgcaggagt aatcattttt gctctccgtt acaggcgggc
     6901 atgatgcccg ccgtgagttg attagctgtc ggcgaacttc aggccagtaa ccatcgcgca
     6961 cttgccatag tcttcaacga cataagcgtc attgatggac tggtaggtgg cgatgcggtt
     7021 gtattccggc tcgtctttca tcaggcgacg cattgaacct ttctgccagt aaatcgacag
     7081 gttgttgaac gaggtgatca gcatcgttgc atccgggaag aacggcgcaa ggaacacgcc
     7141 cagcccgcca atggtgcgcg atgacaggat gagctgcccg gcgagtaatt ccgcattggg
     7201 attctggccg ctgatgctgt tcagcacggg cagacgcagc gagttaaaca ggttgcgccc
     7261 cataatcacc acgaggtcgt cagcttcctt gtgccattca tccagcaggg atgaacgcgc
     7321 gtcctgtacc agtgcatcag cgttcgcata cttacccgcg tgcgccacgg tgttgtccat
     7381 gttgcgggag gtcagcgtca cgtcattcat aacgcgttcg ctggcgtcgg ttctgatgtg
     7441 ctccagccag cccacgttaa cgtcctgaag cagcttgttg gtgctgaagt tggactcatc
     7501 cgcgtgagac gtgccgttga aaccgatcat gatgcggtca agcgccacct gtcgggcaat
     7561 ctgtgcgctg atgcgggact gaaaatcagg gtgtgccgac caggcatcaa gctgcggata
     7621 cgaaataaac gtgtcgtagt tcacctgttc acactgatat ttgcggtttt tcagatcaac
     7681 cacgttattc gggttacggc gttttgtgcc gtcataactg gtattcgtgc gcgcaatcgg
     7741 tcctgtggta tccaggagga ttttttcgcc tttctggtcg gtcacgccga tcacgttatt
     7801 tttttcgtaa attcggtact ttcctttgag gcgttttcaa aacgctgctg caccgcgggt
     7861 tccacggtaa atcgcgacac cagtgcagat accgggatat tattaagcga cacctgctgc
     7921 gccatatagc aacccagctt gttgcgggta atatctgaca tcaccagatt cataaaaaat
     7981 ttgctccttt gtctaatcag aagtcggcca gctggtcgga ggctgcgccc gttgcggtga
     8041 agcggttctg cggatcgccg tcctgcgtgc gcagtttttc cttcagtgct gccagctctg
     8101 tggtcagtga cgtaattttc tggcggtcct gctgatggcg ggtttccagc acattaaaac
     8161 ggtcgataat gtcggcctgt gacgttgcga cgccttccac cgcttcctga atacgggaaa
     8221 aactggcgtc atccgctttg cggccacggc caataatccc cattacgcgg ttaaaccact
     8281 gggtgccttc ttcctggcgt tgttctgcca tttcgatgat ttcagactcg atggcttcgg
     8341 agataagcgg cgcttcaccc tggatactgt taaacgtcat caccgcctga cgttgctgtg
     8401 ccgtgaattt caggcgctca gtgcccaggc ttgccggggt gtcggtcatc gccagcccga
     8461 ccagataggc gcgcccgtta acggagaact gcgggtgcag ttcgatactg gaatagattt
     8521 tcttgccgtc cgcaacaagc tgcttcatgc gctcggtcgg ttcgatttct gcatacagcg
     8581 cagtacgtcc ggccagcgga ccttccgtaa tctcttccgt actcagtgcg gtgacatcgc
     8641 ccattgcgga aaattcgctt gacgggcatg gcgagagata gtgctcaacg ttcacgcggg
     8701 cagcgtaaac atccgggttg aagttctcgg cggcttcacg cagatgcacc ggactgattt
     8761 cacggccatc aacagttgat ccggagacag ccacgcgaaa ctttttgcgg gatgtctttt
     8821 tttcattagc catagttttt gcccctctga ctggttcttc agtcatgatg gcaaagcgta
     8881 acaggctgat acaaagggct tttgttgtaa gaaaacggcc agaacagagg gttaaggaga
     8941 acagtttcgc gcgcgggtaa tcttcctgta attactcagg gggagcaatg attcaggacg
     9001 cttttgtgcg ccagcgtgcg cggcaacttt actggcaggg ttatccgccc gcagaaatat
     9061 cacgtctgat gggaataaac ccgaacacga tttatgcgtg gaaaaaacgt gaccagtggg
     9121 atgaaacgcc acccgggcag cgtgtcacgc agtccatcga tgcgcgcctc atccagctta
     9181 ctgaaaaaca gaataaaaca ggcggtgact ttaaggaaat agacctgctg acccggcagc
     9241 ttaaaaagct gcatgatggc cagccggatg tgatggccgc aggaaagaaa ggccgggcga
     9301 aaaaactcaa aaatcatttc acgccggaac agattgccgc actgcgggaa aaaatcatca
     9361 gcaggctgga gtggcatcag cggggctggt ttgactccct gaccctttgc agggaagccg
     9421 ggatacgtaa caggatgatc ctgaaatccc gacagattgg ggcgacctgg tattttgcac
     9481 aggaagcact gctgatggcg ctgcgtgacg atgtggcgca accttaccag cgtaaccaga
     9541 tttttttgtc tgcgtcgcgt cgtcaggcgt tccagtttaa aagcattatt cagaaggccg
     9601 cggctgaagt tgatgtggag ctgaaagggg gcgataaaat catcctctcc aacggcgcag
     9661 agctgcattt tctcggcact tctgctgcgt cggcacagtc ctatacgggc aatttttatt
     9721 ttgatgaatt tttctgggtc agtcgctttg ctgaactgcg caaggtggct ggcgctatgg
     9781 caaccctcag cggactgcgg cgcacctact tctccacgcc atccaccgaa acgcacgagg
     9841 catacgccta ctggaacggc gaccgctgga acgagaaaaa ggcctcgcat aaacgccagc
     9901 gtttttctgt ggactggaaa acgctgcata acgggcttat ctgccctgac cggacgtggc
     9961 ggcaaattgt cacgctggaa gatgtggtta atcacggctg gaaacacacc gatatcgacg
    10021 aaattcgtga tgaaaacacc gaagacgagt tcctcaatct ctatatgtgt gagtttgtcc
    10081 gcgaagggga atcggcattt aacctgaata tcctgattgg ctgcggcgtt gacggatacg
    10141 acgactggaa agactggaaa ccttttgctc cccgcccgat ggggaatcgt ccggtatgga
    10201 ttgggtatga cgcaaacggc agcagtggca acggcgacag cggcgctgtg tccgtggtgg
    10261 ttcctccgtc tgttcctggt ggccgttttc gaacggtgga gacgcgacgc gttcaggggc
    10321 tggagtttga agaacaggcc agagtcattg aagagttcac gtgtcgctac aacgtggaac
    10381 acatcggcat tgatgtgacg ggcgggaacg gggaggctgt ttatcagata gtgaaacggt
    10441 ttttccctgc cgctattccg tacaccttca cgctgtcatc aaaacggtcg ctggtactga
    10501 aaatgctgca aataatgcgt gccgggcggt gggaatacga tcgcgccgaa cgcgagctgg
    10561 tcgcggcctt taacgccgtg cgtaaggtga aaacaccggg cggctttatc acttacgaaa
    10621 cggaccgagc gagggggatc agccacggcg accttgcgtg ggcaaccatg cttgctgtca
    10681 ttaacgaacc gattggcggc gaaggagaaa acgagcgttt cacggttatg gagttctgat
    10741 gagcagaaaa aataaaaaag tgcgcatgag ttcacgcatt gatctcgctg atgcgctcag
    10801 gaaagaatca tcgctcagtg cattcacatt tgatggtcct tatcgcctga ccgggcatga
    10861 cctgctggac aatatgtact gtgctgataa cgggcggtgg tatgaaaccc cggtggactg
    10921 gtacggtctg gcaagagctg cccggcaaac gtcctggcat cagtctgcgc tttattttaa
    10981 gcgcaatgta ttgctcggtt gctacatccc gcacccgctg ctttcccggc aggatttctc
    11041 ggcgctggcg ctggactggt ttgtgttcgg taacgcattc cttgagcttc gaagcaatat
    11101 gctcggcgaa ccgcttaaat tacggcacgc cctggcgaaa tacatgcgac gcggaagcga
    11161 tcttgaatca tggaggtatg tgcaggatgg caaggatgcg tttcagtttc gcccaggcaa
    11221 agtgtgtcac ctgatgaatc ctgacattaa ccaggaaatc tacggcatgc cggaatatct
    11281 cggcgcatta ctctcggcca gcctgtctca ttcggcggac atgttcagaa aactgtatta
    11341 cgacaacgga tcccacgccg ggtgcatcat ctacatcggt gccgcgcagg taaaccgcga
    11401 aagcatggac tccctgaaag aaacgctaca gggtgcgcgt ggtggtggtg cgtttaaaaa
    11461 cgtgctcatt catgcgccca acgggggcaa agagggggtg caaattttgc cgttccagca
    11521 gatcaccgca aaggatgagt tcatgaatgt taaggcggca tcccgtgatg atgtgctggc
    11581 tgcgcaccgc gttccgccgc aactgatggg ggcgatgccg ggtgaaaaaa gtgcgtttgg
    11641 tgatgtggag aaggccgcgc gggtttacgc aattaacgag ctgatgcccg tcatggaggc
    11701 catgaagcac atcaatgact ggcttggcga agaggtgatc cgctttaacc cttacgcact
    11761 gttagacatc cagcccacat cctgacgcgc ttcgcttgtc tgctgcttcg ccggggcata
    11821 aaaaatttat gccccgactc tccagctcct gtatcagtca gataatttca cgacgccttc
    11881 cagtttatcg ccaccatcga cggtcagact cttacgcaat cccaccgcgt tgactgcatg
    11941 ttctcgccgc ctcagtgcga ttttgacggc cttacctttc accccatcaa atcagaatcc
    12001 ctcacgtatt tttcacgctc agcgtgagaa atacggccat tctgtcgtgt cgctgcgaca
    12061 tcgttaaggg aacgctattt accccctgaa acgcgggctg ttcccccgtc acctgcgcgc
    12121 agaaaaagcg cgtttttttg tgcacgcacg gatccttgac ggatccagcc gccacgcggg
    12181 ctggaagggc aaaaattcgt tcaaaaaaat tgtgcaaatt tgtgcactat tgtgcagcag
    12241 ggcgatgtgc gattatcgcc ctggtttgag ggggatcagg tgttattttt atctgtcgtc
    12301 agtgggctaa accatccaca atttacatcg catgactgat gcgttctgtc ctcgtgtttc
    12361 tcctgccaca atgcttttcc gccagccttt atgatggctc tttcaacatc ccgctcgtat
    12421 cgcaagggct gaaatacgtc attaatgaaa tattcgctta tgtccggaag cgatactgca
    12481 aaattatttg tttgttgcgc cgtcctgcta tgcagcaaag cggcatgctt taatgcgtcc
    12541 tgtgctttct ggaatttaag tgcatagtca gtggcgattt tttccagttc agcgattcgg
    12601 ttgtctttgg tttccagcca atcaagtagc gccaatactt caggatcccc gacatccaac
    12661 actgttacgc gtgatttttc ataatgttcg tcagcaagag ttcggccaat tttgaaatct
    12721 ccatcatcac cataaccgga gcaggcataa acaacctgtg ctccagatat tcgctgaatt
    12781 gacatttcct cgccgcaaat agggcatttc ggtactggca caggtgaata acgttcacgt
    12841 aatgcctgat agttgatttc gctcattaag tcgttctcct tgtttatgtt ctgaggtgga
    12901 gagcagggcg aactcgccct gatgttgtgt tattcaggaa ataacgcccg gatatttccg
    12961 gccatctgac tggttatctg tgcggttgat actggctgtg atgcggggcg ttctgtcctg
    13021 gtttgtgtca ctgataacgc ttcatcacca gcccatgcag ccagtcgata agcctctgcc
    13081 ggattcattt tcagaagtgc cagcccggcc agaaaagcca cgcgttggcc gcttttgcgg
    13141 gcttctggtg taaggctgtc cagccaggcg catgcttctc cttcgttctt gacggcggtg
    13201 ggcttcagat agaaacttat ccgtctggtt ggagtcgtca ttggtttact ccttgtccat
    13261 tgcgtacagc ccattaacca gagcaaactg tggcaccccg tccgcgatga aagtcgcatt
    13321 aactccgcag gcttcgcgga tagcgggtgc cacaatctcc gcccctccac cgacaaccat
    13381 cacccgcccg taacccgaaa aaaccgccag cgcgcggatc acgcgttgtt tcagtgtttc
    13441 ttccttttca cgaattaccg ccatcaggct ggcgtaatgc gcgtcattgt ggatgtgctg
    13501 gcgcagccag gcttcatcat ggcgatgttc gataatggta ttggcgatgt ggtgactggt
    13561 gcgcataccg ttagtggcca tcaccgacag tacggcatcg gccatcagag aaacgcccac
    13621 gtgtggatcg caaaacacct ggctgatacc tgccagttgc ccctgaacct ttgccacatc
    13681 cagcgtggtt ccgcccaaat ccacaatcag cagggattca aacggactca tgtcagccag
    13741 tgccttaaag ccagccggaa tggattcagg cataacctgt acgttacgga tagtgaatgc
    13801 ttcgccgttc tggtac
//
LOCUS       DAJVTY010000108        13701 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVTY010000108 DAJVTY010000000
VERSION     DAJVTY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.108"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            211..1158
                     /locus_tag="OQQ66_002817"
     CDS             211..1158
                     /locus_tag="OQQ66_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1905683.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1237..1313)
                     /locus_tag="OQQ66_002818"
     tRNA            complement(1237..1313)
                     /locus_tag="OQQ66_002818"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1277..1279),aa:Met,seq:cat)
     gene            1522..2619
                     /gene="mltA"
                     /locus_tag="OQQ66_002819"
     CDS             1522..2619
                     /gene="mltA"
                     /locus_tag="OQQ66_002819"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1905684.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2696..3502
                     /gene="tcdA"
                     /locus_tag="OQQ66_002820"
     CDS             2696..3502
                     /gene="tcdA"
                     /locus_tag="OQQ66_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1905685.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3553..3996)
                     /gene="csdE"
                     /locus_tag="OQQ66_002821"
     CDS             complement(3553..3996)
                     /gene="csdE"
                     /locus_tag="OQQ66_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1905686.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(3996..5201)
                     /gene="csdA"
                     /locus_tag="OQQ66_002822"
     CDS             complement(3996..5201)
                     /gene="csdA"
                     /locus_tag="OQQ66_002822"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1905687.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5393..5620
                     /locus_tag="OQQ66_002823"
     CDS             5393..5620
                     /locus_tag="OQQ66_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1905688.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5971..6888
                     /gene="gcvA"
                     /locus_tag="OQQ66_002824"
     CDS             5971..6888
                     /gene="gcvA"
                     /locus_tag="OQQ66_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1905689.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6907..7302
                     /locus_tag="OQQ66_002825"
     CDS             6907..7302
                     /locus_tag="OQQ66_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1905690.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7295..8395
                     /gene="rlmM"
                     /locus_tag="OQQ66_002826"
     CDS             7295..8395
                     /gene="rlmM"
                     /locus_tag="OQQ66_002826"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1905691.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8439..9170)
                     /gene="fucR"
                     /locus_tag="OQQ66_002827"
     CDS             complement(8439..9170)
                     /gene="fucR"
                     /locus_tag="OQQ66_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1905692.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9228..9650)
                     /gene="fucU"
                     /locus_tag="OQQ66_002828"
     CDS             complement(9228..9650)
                     /gene="fucU"
                     /locus_tag="OQQ66_002828"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1905693.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9652..11070)
                     /gene="fucK"
                     /locus_tag="OQQ66_002829"
     CDS             complement(9652..11070)
                     /gene="fucK"
                     /locus_tag="OQQ66_002829"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS1905694.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11067..12812)
                     /gene="fucI"
                     /locus_tag="OQQ66_002830"
     CDS             complement(11067..12812)
                     /gene="fucI"
                     /locus_tag="OQQ66_002830"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1905695.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(12845..>13701)
                     /gene="fucP"
                     /locus_tag="OQQ66_002831"
     CDS             complement(12845..>13701)
                     /gene="fucP"
                     /locus_tag="OQQ66_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1905696.1"
                     /translation="LNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLS
                     AYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLAR
                     IRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFT
                     GTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLG
                     IKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFAR
                     FRSQTATN"
BASE COUNT         3301 a         3364 c         3611 g         3425 t
ORIGIN      
        1 accgtcatgc tcgtctactg ttgttctggt tgttggtgat gcggtggcgc ttgcactctc
       61 tgaattgaaa aaatttactc gtgctgattt cggcttatat catccaggag gtgcactcgg
      121 cattaaagca aattcataat ttcaaagtat aaatacagtg accggtcacg ccatcttttg
      181 gggtggcgtt cattgaagtg agtgtcagct atgaaaaatg ttctggtcac cgtccccagc
      241 ttttcagcac gctgtgtttc cgccagcaag ttgctaagag agaataattt taatcttatt
      301 attaagaata atgtggagca tctgcttaaa tcagaatcca ctgcattacg agaatctatt
      361 tgcgcggtca tcgccggaaa agattgttat caggcggaca cgctatcatt gctgcctggt
      421 gtgaaaataa tatcgagatt cgggacgggg atagataata ttgatcttcg tgcagcacaa
      481 caatccggta ttgtagtcaa taatgcggtt gggattaatt caaatgctgt cgcggaattt
      541 atcattggac ttatcttcgc gagcatgaga aatattcccg gcagctatca tgcgatgcaa
      601 aatggctact ggggtgagtc gcatggctat gaattacaag gcaaacggat tggcatggtt
      661 ggctacggca atataggtaa aaccctggca aaaaggttat ccggttttga tgttgagctt
      721 ttagcttatg acaaacaacc cgattatcag gttgctgaca aagttggagt tcagtttgta
      781 tcaattgaag atatctttat gcagtcgcat gtcatcattg ttcttttgcc tttttctcct
      841 gagctggaga acttcattag tcataaatat ttgtcgatga tgcgcaatgg tgctctaatc
      901 atcaatgccg caaggggaaa gttgctggat gagggcgctt tactacaggt gattgaagaa
      961 cgaaatgtgt ttgcggcgct ggatgtgttt agctctgaac cgttggcgca atttagccca
     1021 ctgctacatg ccaaaaatat cattacaacg ccgcatattg cggccgccac cgttgagtca
     1081 taccagcaaa ccggtacaca cgttgcccag tcgataattg attacttcgc aggaagggag
     1141 ataaaaaacg tgttgtgagt tcagcgaacg aagtttttaa atacaaaaaa aacgcaattt
     1201 gtggaacaca ttgcggttta tcagcattct aagtattggt tgcgggggcc ggatttgaac
     1261 cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc gcgtccgtgg
     1321 atgcgcacta tactccgctc gcgttttcat gcaacctttt ttttaccccg atcacggatt
     1381 tagcatgatt ttgaacaaaa acaaaccact aaagcctatt ttttgtgcaa aatttgcgtc
     1441 agggagaggc tgctcatttc attaaacgcg gcggtttgtt atcttcgttg cgccttattt
     1501 tttaacctga agaagagaac aatgaaagga cgttgggtaa agtaccttct tatgggcacg
     1561 gttgtggcaa tgcttgccgc ctgctcttcc aaatcaaccg atcgcggaca gcaatataaa
     1621 gacgggaaat ttacccagcc tttctctctg gtgaaccagc cagatgccgt tggcgcgccg
     1681 attaacgccg gtgattttgc cgagcaaatt aaccatatcc gtaattcgtc accgcgtctg
     1741 tatggcaacc agagtaatgt ttataacgcg gtgcaagagt ggctgcgcgc aggcggtgat
     1801 acccgcaata tgcgccagtt cggcattgat gcctggcaga tggaaggtgc cgacaactat
     1861 ggtaacgtgc agtttacagg ctattacacg ccggtaattc aggcgcgcca tacccgccag
     1921 ggcgagttcc agtatcctat ttaccgtatg ccgccaaaac gtggtcgtct gccgtctcgt
     1981 gcggagatct acgcgggggc attgggcgat aaatacatcc tcgcttacag caactctctg
     2041 atggataact tcattatgga tgtgcagggt agtgggtata tcgactttgg tgatggcagt
     2101 ccgcttaact ttttcagcta tgcagggaaa aacggtcatg cctatcgcag cattggtaag
     2161 gtgctgatcg accgtggcga agtgaaaaaa gaagatatgt cgatgcaggc gattcgtcat
     2221 tggggcgaaa cacacagtga agccgaggtt cgcgagctgc tggaacagaa cccgtctttc
     2281 gtcttcttta aaccgcaatc ttttgcaccg gtgaaagggg caagtgcggt gccgctggtt
     2341 ggccgtgctt ctgttgcctc cgatcgttca attattccgc caggtactac cttgctggca
     2401 gaagtgccgc tgctggataa taacggcaaa tttaacggtc agtacgaatt gcgtctgatg
     2461 gtggcgctgg atgtcggtgg cgcaatcaaa ggccaacact tcgatatcta tcaagggatc
     2521 gggccggaag ccggacatcg cgcaggttgg tacaaccact atggacgtgt ctgggtgctg
     2581 aaaaccgccc cgggcgcagg taacgtcttt agcggctgat gtgttattct gtataccgtt
     2641 tttacggata cgacagggtg agggataacc tcaccctttt taattctgag gtgctatgtc
     2701 tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc acagcgcgtc tgtatggtga
     2761 aaaagcgttg caactgtttg ctgacgcgca tatttgtgtg gttggtatcg gtggtgtcgg
     2821 ttcctgggcg gcggaagcgc tggcgcgcac ggggattggc gcaatcacgc ttatcgatat
     2881 ggatgatgtg tgcgtcacca ataccaatcg gcaaattcac gctctgcgcg ataacgtcgg
     2941 gctggcaaaa gcggaagtca tggcggagcg cattcgccag attaacccgg agtgccgtgt
     3001 aacggtggtg gatgatttcg tgacgccgga taacgtagcg caatatatga gtgccggtta
     3061 ttcgtacgtg attgatgcca ttgatagtgt acgacccaaa gcggcgctga ttgcttattg
     3121 tcggcgcaat aaaatcccgc tggtcacgac cggtggcgcg ggtgggcaga ttgacccgac
     3181 gcagattcag gttaccgatc tggcgaaaac gattcaggac ccgctggcgg cgaagttgcg
     3241 cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt ataggtaagc tcggcgtgga
     3301 ttgcgtgttt tctactgaag cgctggtgta cccgcagtca gacggtacgg tgtgtgcgat
     3361 gaaagccacg gcagaagggc cgaagcggat ggattgtgca tctggatttg gcgcggcaac
     3421 gatggtgacc gccacctttg ggtttgttgc ggtttctcat gcgctgaaga agatgatggc
     3481 gaaagcagcg cgtcagggtt aaacaatcag gtcggatgcg acgcgaacgt cttatccgac
     3541 ctggttctgt gcttaaacct gcttcgccgc agcgataatt gcctcgctta acgcattcaa
     3601 cccctggctg cgtgaggcgc taagctgccc acgcaatccc agctcatcaa acaatgccag
     3661 cggcgactgt gcctgtaatt cggcggcggt tttcccctca acggcagtca acaacaccgc
     3721 caacaggccg agcacaatgc gcccttcgct gtcgccaaag aagtgcattt taccgttttc
     3781 agccacggta tatcccagcc agacgcggtt ttcgcatccg gcaatctctt tagcctgcgc
     3841 ttttaactcg tctggcaaag ccggaagctg tttccccagc atgatcaact ggcgatattt
     3901 atcttcccat tgcgtcaacg gtgcgaaggt attgcgtaac gtttctgcgg ttacggttgt
     3961 gccgaacgga tgtccggcga attgcgggtt tgtcattaat ccaccaataa ttccagcgcg
     4021 cggtcaacgg cattcaccag cgcatccaca tcactctttg tattatatgg cgcaaaagag
     4081 gcgcgcagtg tgccggttac gcctaattct gccagtagcg gctgagcaca atgttgcccg
     4141 gcccgcaagg caataccgta ctccgccagt agcgtcacca tatcgctgtg atgaacgcca
     4201 gcaaaatcaa aggccagcag gctggaatcc tggcagcgga atgaacgaaa gcctggacgt
     4261 ttcgccagcg catcttccgc cagcgttgct aagctatggc tccagttttc ggcctggttg
     4321 atatcgtaat ctgccagcca ttccagcgcc gcgcttaatc ctatgacacc agccacattt
     4381 ggcgttcctg cttccagttt ccacggcgca gattgagtcg tgaagccgtc aaaactcact
     4441 tcgtgaatca ttttgccgcc acccagccag ggcgacatcg cttccagcag ttctgatttg
     4501 ccatacagca cgccgatacc cgtcgggcca tacagtttgt gaccagagaa agcatagaaa
     4561 tcgatatcca gttgctgaac atccgcgggg aaatgcaccg ccccctgagc accatcaacc
     4621 atcaccacca tccctgctga atgagcaaag gtaatcgctc gcgccagatc cgggcaaccg
     4681 ccagtaacgt tcgacatctg actcaacgcc agaatccgac tacggggagt aatcagttct
     4741 ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg gcaatttcac cactttggct
     4801 ccagtttgtt gggcgaccat cagccagggg acgaggttgg cgtggtgttc tgccacgctg
     4861 acaataatct catcgcccgg ttgcagacgc ggacgcgcat agcattgtgc caccatgttg
     4921 atggattcag tggtgccgcg cgtccagacg atagttttat catccggtgc attcagtaat
     4981 tgcgccactt tctctcgtgc agcttcataa cgcgcggtca ggcgttgggc ttcggcaaac
     5041 tggctgcgat ggacgtttcc ggcgctcagg ctgtaaaact gtcgagtggc ttcaaccacg
     5101 gcttcaggtt taagcgcggt cgcggcgctg tcgagataga cgcccgcatc ctgtagtgcg
     5161 ggaaactggg cgcgaaactg cgcgggatta aaaacgttca tggtactcct cggcttgatg
     5221 caccgatcgt gacgcaaaat tcactttgat acaaggagag tgctaaccat cagaattatc
     5281 tggatatcct ggcgaagccg cgtaagtaac atatgctgaa tagtgttagg cgttaatttt
     5341 agcctgttaa tcaatactat taagatttat taatcgcttt aaggagaaga tgatgaaaaa
     5401 gactgccgca attatttctg cctgtatgct gacttttgcc ctgagcgcct gttccggttc
     5461 gaactatgtg atgcacacca atgacggacg taccatcgtc tctgacggca aaccacagac
     5521 tgataacgat accggtatga tttcgtataa agacgctaat ggcaacaaac agcagatcaa
     5581 ccgtactgac gtgaaagaga tggtcgaact ggatcagtaa ttcgcgatcg caaggtaaaa
     5641 aaaagcaccg caattaggcg gtgctacatt aatcactatg gacagacagg gtaaatgtac
     5701 aggaagtgaa aaaaggtagc tttgctacca tggtctgaat cgcagaccaa ttgcaaacac
     5761 aacaacacaa catcacaacc gtaagccaaa agttcaccag aacacgcatt ccgataaaac
     5821 ttttcgttcc ggctcaggaa gtgccgccac tataggtatt tgctggtaga agctcaacgg
     5881 acaatttata atggctcaga ttaaaaaaac taataggtta catagtgtga tctaattgtt
     5941 aaattcattt aacatcaaag tttaatagcc atgtctaaac gattaccacc gctaaatgcc
     6001 ttacgagttt ttgatgccgc agcacgccat ttaagtttca ctcgcgcagc agaagagctt
     6061 tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc ttgaggattt tttggggcta
     6121 aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg aggaagggca aagctatttc
     6181 ctcgatatca aagagatatt ttcgcaatta accgaagcga cgcgtaaact ccaggcccgt
     6241 agcgccaagg gggcgttgac ggtcagttta ctccccagtt tcgccattca ttggttggtt
     6301 ccgcgacttt ccagctttaa ttcagcttat ccgggaattg acgttcgaat ccaggcggtt
     6361 gatcgtcagg aagataagct ggcggatgat gttgatgtgg cgatatttta tggtcggggc
     6421 aactggccgg ggctacgggt ggaaaaactg tacgccgaat atttattgcc ggtgtgttcg
     6481 ccgctactgc tgactggcga aaaacccttg aagacaccgg aagatctggc taaacatacg
     6541 ttattacatg atgcttcgcg ccgtgactgg cagacatata cccgacagtt ggggttaaat
     6601 catatcaacg ttcagcaagg gccaattttt agccatagcg ccatggtgct gcaagcggct
     6661 atccacgggc agggagtggc gctggcaaat aacgtgatgg cgcaatctga aatcgaggcc
     6721 ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta aaaacgcttt ttatctggtt
     6781 tgtcatgaca gccaggcaga actgggtaaa atagccgcct ttcgccaatg gatcctggcg
     6841 aaagccgctg ctgaacaaga aaaattccgc tttcgttatg aacaataatt tacgtagggt
     6901 acgaccatga ccagccgttt tatgctgatt ttcgccgcca ttagcggctt catttttgtg
     6961 gctctgggcg cttttggcgc gcatgtgtta agtaaaacca tgggggccgt tgagatgggc
     7021 tggatccaga ccggcctcga ataccaggcg tttcatacgc tggcgatctt aggtctggcg
     7081 gtggcaatgc agcgtcgcat cagtatctgg ttttactgga gtagcgtttt cctcgcgtta
     7141 ggcacggtgt tgttcagcgg cagcctttat tgcctggcgc tgtcccatct gcgtttgtgg
     7201 gcgtttgtca ctccggttgg cggcgtgagc ttcctcgcgg gctgggcgtt aatgttagtt
     7261 ggtgctatcc gtttaaagcg caagggcgta agtcatgaat aaggttgtat tgctgtgccg
     7321 tccgggcttt gaaaaagagt gcgccgcaga aattaccgat aaagccggcc agcgggaaat
     7381 tttcggtttt gcccgcgtga aagagaattc gggttatgtc atttatgaat gttatcaacc
     7441 tgatgatggc gataagttaa tccgtgagct gccgttcagt tcattaattt ttgcccgcca
     7501 gtggtttgtg gtgggggaac tcctgcagca tttgccgcca gaagatcgta ttacccccat
     7561 tgtcggcatg ttacagggcg tagtagagaa gggcggtgaa ctgcgtgttg aagttgccga
     7621 taccaacgaa agcaaagagt tgctgaaatt ctgccgtaaa tttactgttc cgctacgcgc
     7681 tgccttgcgc gatgccgggg tgctggcgaa ctatgaaacg ccgaagcgtc cggttgtgca
     7741 tgtattcttt attgcaccag gctgctgcta taccggttac tcatacagca acaataattc
     7801 gccgttctat atgggcattc cgcgcctgaa atttccggca gaggcgccga gtcgttccac
     7861 gctcaaactg gaagaggcat ttcatgtgtt tattcctgcg gatgagtggg atgaacgcct
     7921 ggcgaacggg atgtgggcgg tggatttagg cgcttgccct ggcggctgga cctaccaact
     7981 ggtgaagcgc aacatgtggg tttattccgt cgacaacggc ccgatggcgc aaagtctgat
     8041 ggataccggg caggtgacgt ggctgcggga agacggtttc aaattccgtc cgacacgcag
     8101 caatatctcc tggatggtat gcgatatggt tgaaaaaccg gctaaagttg cggcattgat
     8161 ggcgcagtgg ctggttaatg gctggtgccg tgaaaccatt ttcaatctca aactgccgat
     8221 gaaaaaacgc tacgaagaag tatcacacaa tctggcgtat attcaggcac agcttgatga
     8281 acatggcata aatgctcaga ttcaggcgcg gcagttgtat cacgatcgcg aagaagtgac
     8341 ggtgcacgtc cgccgcatct gggctgcggt gggtggtcgt cgcgacgagc gataacaggc
     8401 aaggccgggg cgtcgtcttc ggcctgctac cctttccgtc aggctgttac caaagaagtt
     8461 gcaacctggc gctcatcaga aataatgtgc gttacctcat ccagatgccc gatgcgggct
     8521 tcccctgaac gattaaattt acttttatca atcaataaca acgattgcgc ggcacgtttt
     8581 aatagcatcg atttgtaatc agcgttgatc gcattggagt cccacagtgc gccgctgcta
     8641 tcgatccctt cacaagaaaa aataaacaga tcgatttcca gcgatttaag ttgggaaatc
     8701 agcgagggat tgacgtaaca gccatatttg cgctcaagtg tgccgccgga actgatcagt
     8761 tgaatgcgtt cgcgtttacc gagttcatag caaatcggat ggctattggt gaagacctga
     8821 atgttgatgt caggcaactg gcgtgccaga taccagcaag ttgaactggc gtctaaggct
     8881 atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg cgatatctgc tttatgcgca
     8941 taatggcttt tcagcctgat gtgaaaggga tcgccactgt cttgattttg acggtggata
     9001 tatttagcgc gtccatgatt gcgcagaatt ttaccctgcg tctgtaattc attgagatcg
     9061 cgacgaatgg tttctttact gacctttagc tgttcagaga gagcttccgt ggtcaggctg
     9121 gtatggttca gcagcaggtc gactatcgct tgctggcgtg ccgctttcat atttttcacc
     9181 ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg tatgagatta cggtgttacc
     9241 ccttttttta aaagaatatt cccgtacttc gctcgttcgc ctgtgataac gatcgcaaag
     9301 gctttttgcg cccgttcata aaacgcaaaa cgattgatgc ggatgatgtc aggacacggg
     9361 gcttgtagtg aaagcgcatt acggtaacgt cgttctactt caggatcgag agtgtcacct
     9421 tctaccgccg ccatcatcac cagcggcggt gcataactgt ccagttcaaa taacgggata
     9481 atcgcctgga gcaagtcgct caccaacagg ccatcagcgc ggatcacctg cggtcccatc
     9541 gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat ctccatgtcc catctctgcc
     9601 agcactttca atagttcggg agaaattaac ggcgaaattg ttttcagcat ttcacacttc
     9661 ctctataaat tcaggttcag tttgcgggta gaaataacgg tactgataat gaatctgtgc
     9721 gcgggcttct tcagggctgt taaattcccc tacgccatac caaccgaaca gcgcagctcc
     9781 tgcgacggtc gtttcggcgt cgtcgagaac ttttaccgga atatcaagca tattggcttt
     9841 aatctgattc cacaatgtgt tgcgacttcc tccaccgact aacaataatt cagaggcctt
     9901 aaagtgcccg attttttcca gcatctgtag attgcgctgt aattgcgcag ttaacccttc
     9961 cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc gtcactcctt gccagccagc
    10021 gttctgacac gacaataaat cacactgcat ttttacgcca tccgcgctag gcgcgataag
    10081 acgagcttct tcaatcaaca tttgccaggg tgtttcagcc gtccagaaca gttttctcac
    10141 ccattccagc acgccggatg ccagccattg catacctggg ttatacaacc ctgcctggct
    10201 atccagttcg caggtggaac cggcgtactg acttaacagc gaagtatcaa cctgggcgct
    10261 acggaccatt aaaatttccc atgtaccgga agagagcacg ggttcatttt ggtcggcacc
    10321 agcgccaaaa agggcgaact gggtatcgtg acctgcggaa atcaccggta tgccaacggg
    10381 taagccgagc attgctgcgg cgctgttctg tagcgtacca atctgttcac ccgcttccac
    10441 cagacgaggg aagagtcggc gtggaatacc agtggcttgt aaaatttgcg gactgaaatc
    10501 gcgttgctgg atatccagca tctggctggt tccggccatc gtgatatcag tagtgaattc
    10561 gccggttaaa cggtggttaa tcagcgacga aataaagaac caggcgtgcg tgcgttccag
    10621 cagttgtgga tgattttctt tcaaccacac caacttatat aacgtattga aactaaaggc
    10681 tccgacgcca gaaatagcct gcaaccgctg tgcggagatc aactgttcaa tattgtccat
    10741 aaccgctgct gttcgcggac atttccagct aatgatcgga tagagcagat tgccttgctt
    10801 atctaccaga gcgccatcca caccaaaggt ggtgacggcg ataccgcgga tgtggcattc
    10861 agtcagttca ctattgattt gccgacagca atcagcaaag cgttgcaaaa tggcgtctaa
    10921 agaccactgg tgccaggtgt tgttttccat cgcgatatcg ctggcattag gcgttgaggc
    10981 gcgggcaaca atttttccct gccgattaac cgcgatggcc ctgacattgg tcgcgccaca
    11041 gtcgagtacc aggataactt cttgtttcat aatcgctcct gcaagacttc aatatccatg
    11101 ccgtgcgcag cccaggcaga aggacgatac actttggtct cttcaacgtt gtgcatacat
    11161 accgggatac gcagcatgga ggcgagagtg ataaagtcag cgccaacgtg gccgatggtc
    11221 agaaccccat ggttagcgcc ccagttcgcc attaccgagt acacatccgt aaacggccct
    11281 ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt gcgtttgttg
    11341 aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat ttgcagtacc
    11401 ggtcccaggc ctttgatgat gttgacacga gtcatggtga acgggacgcc gccttcggta
    11461 aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg gcaccattcg
    11521 gtagcggcga ggcaagcgtc agcctcttgc tgagagattt cccagtgcgg cttcatcgtc
    11581 gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga acctgagttg
    11641 atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt tatgccccgt tacacgctca
    11701 attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt gccggtgagc
    11761 tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc cacgacaaag
    11821 ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga tcgcttcggc ggtgtcacca
    11881 ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag
    11941 ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg catcatgtcg
    12001 cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg ctcggcattg
    12061 cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc agcccaggcc
    12121 agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg acgcagttcg
    12181 gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt gtgatcaacg
    12241 atggaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt acctttcatg
    12301 ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac atcggcagga
    12361 atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct
    12421 ttctggctgt gagctgccag agccgctgcc aggtaaacag cgccgggacg ttcagtgccg
    12481 ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc actgccatag
    12541 caccagcaag gcgttaccgt aatggtgagg cctacattct ggctgctgaa tttttcttcg
    12601 caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca ctcgacggca
    12661 gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt cgccatattc
    12721 attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg
    12781 ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta ttcggaaaat
    12841 atgttcagtt agttgccgtt tgagagcgga aacgggcaaa gataaagatg accgcgaagc
    12901 agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca
    12961 tgaccggagt gacaataccg ccgccaataa tggtcataac gatgaaggac gaaccgtatt
    13021 tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac tgaatcgaca
    13081 taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg aaggctgaga
    13141 tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt ggtgcgaagc
    13201 gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg gttccggtta
    13261 aatagttagc ggcaaagcct gcagtcatac ctggaatttc ttctacagcg tagcgaatca
    13321 aatagctcca gcaggccgtt tgtgcgccaa cgtagcagaa ttgcgctaat actgcccagc
    13381 gccagtggcg aatacgcgcc aggcgagaaa gcgatgcgga gaacgatcct tgtttggcgt
    13441 cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc agggcgacca
    13501 gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc aggctgtgtt
    13561 tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat tgatgtggca
    13621 cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca aacgagttaa
    13681 atgtttgcgc aagatttaag c
//
LOCUS       DAJVTY010000109        13660 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVTY010000109 DAJVTY010000000
VERSION     DAJVTY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.109"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..1862)
                     /gene="speC"
                     /locus_tag="OQQ66_002832"
                     /pseudo
     CDS             complement(1..1862)
                     /gene="speC"
                     /locus_tag="OQQ66_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2260..2958
                     /locus_tag="OQQ66_002833"
     CDS             2260..2958
                     /locus_tag="OQQ66_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1905697.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3064..3139
                     /locus_tag="OQQ66_002834"
     tRNA            3064..3139
                     /locus_tag="OQQ66_002834"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Phe,seq:gaa)
     gene            complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OQQ66_002835"
     CDS             complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OQQ66_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCS1905698.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OQQ66_002836"
     CDS             complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OQQ66_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCS1905699.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OQQ66_002837"
     CDS             complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OQQ66_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCS1905700.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OQQ66_002838"
     CDS             complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OQQ66_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCS1905701.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OQQ66_002839"
     CDS             complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OQQ66_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCS1905702.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OQQ66_002840"
     CDS             complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OQQ66_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCS1905703.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OQQ66_002841"
     CDS             complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OQQ66_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCS1905704.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OQQ66_002842"
     CDS             complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OQQ66_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCS1905705.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OQQ66_002843"
                     /pseudo
     CDS             complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OQQ66_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OQQ66_002844"
                     /pseudo
     CDS             complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OQQ66_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12789..13618)
                     /gene="gspC"
                     /locus_tag="OQQ66_002845"
                     /pseudo
     CDS             complement(12789..13618)
                     /gene="gspC"
                     /locus_tag="OQQ66_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         3063 a         3936 c         3521 g         3140 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcaaagtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaactgcgc caacatctct accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acactgatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc cccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cggcgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatctttc gccaataagc
     1381 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag
     1441 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca
     1501 gggcaagcaa aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt
     1561 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
     1621 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
     1681 aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc
     1741 gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag tatctcccaa cgccaccacg
     1801 cgacgatgag aagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
     1861 ataagcgcaa acccgtttcg gggaagtaag aataccggac aaggtggaaa accctgcccc
     1921 atgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
     1981 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagta
     2041 acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc gagagaatgt aaggaggtgg
     2101 cgtgcatcgg gcaaactccg tacagagagg agaaaattcg cgcaatcatg gcacctttcg
     2161 ctaaagcgtg caagccagga ctttgcgacc agacaagcca tcaacaaggc aaaaagttca
     2221 taataagaaa atcaaacaat acagttaaca ggaactcttg tggtcatcgg cccttttatc
     2281 aacgctagtg cagtcttact gggtggcgtt ctcggcgcac tgctcagcca acgcttaccg
     2341 gaacgtatcc gcatctccat gacatctatt tttggtctgg catcgctggg gattggtatt
     2401 ttactggttg tgaaatgcgc caaccttcca gcgatggttt tagctacgct acttggcgct
     2461 ctaattggcg aaatttgttt gctggagaaa ggtgtcaata cagcagtcgc caaagcacaa
     2521 aatctgtttc gtcactcacg taagaagcca acgcatgaat cttttattca gaattatgtc
     2581 gcgattattg tcctgttttg cgccagcggc accgggatct tcggggcgat gaacgaaggg
     2641 atgaccggcg atccgagtat tttaatcgcc aagtcatttc ttgatttctt tacggcgatg
     2701 atcttcgcct gctcgctggg tattgcggtg tcggtaatta gtatcccatt actgatcatc
     2761 cagttaacgc tggcgtgggc tgccgcgctg atattaccgc tgaccacacc gtcgatgatg
     2821 gcagacttca gcgccgtagg cggtttattg ctgctggcaa ccggattacg catctgtggc
     2881 attaaaatgt tcccggtggt caacatgctt ccagcactcc ttctggcaat gccgctttcc
     2941 gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
     3001 ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
     3061 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
     3121 attccgagtc cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt
     3181 tggatctgac aataaccttc acgaaaaaag tttgcttata aagtctgggg gaattactct
     3241 cgccacgtta acgagagtaa ttttattgat actaatctcc tgatacttta cccccgtcca
     3301 aactccagcc gctggacatt caccatccca gtcttctcag cagcactggc atcaatttgt
     3361 gtcacccgca gcgcatactt ttcatccagc gcgtttaacc agttcagcag gtcattaaac
     3421 accaccggat ctatccagac ctgaatattc tccccacgct cggctatccg cttgatgact
     3481 accgagtgcg cggaagcact gtcactgata acccgcgata cctgcgcagg cgttgttgtg
     3541 gcggattttc gcgccgcaat aatatccggc gcggcgctcc tcagtctggt gttcatcgcc
     3601 accagttgct gcaacattgt ctcctgttgt actatccgtt cgctcaacgg ctgccagatg
     3661 aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat gcccttttca
     3721 cgcggagaac gccccgccag gtgttgtgcc agccagtgtt cgccacggct taactggcgt
     3781 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
     3841 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
     3901 atcggttgcc agtgcactac gcagtttgtc gaaactggca aagttcgcgg cccgtagctg
     3961 gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag
     4021 tgacgctgat ttcagggtgc tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc
     4081 ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg
     4141 cttctgctcc gggaacaacg ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac
     4201 ctgttcgctg acgctccata acgtcacgcc ccgctccact gccagcgcaa ctagaatcag
     4261 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg
     4321 ctgccacggc cctgtcagca ggttcccttc cggttcgcca taagtggtaa cggcgggcag
     4381 agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat gctgatagct tttccggtgc
     4441 aatgccgact acggttagcg aaagcggtaa atcctgctca ttgagctggg cgcggaacat
     4501 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcaatgc ggcagataac
     4561 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtctg gcgcgacggt
     4621 agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg
     4681 aatcactgct acggttgcca gttgctggtc gatattcaac ggggcgaaat gcagttcatc
     4741 gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atggtcggta tctgcttacg
     4801 ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     4861 aatcgcatcg gtttgcagcc attgctggag ctgttcgtta gcaacgtcag accagatacc
     4921 gtgctccaca tcgggtgtac ggcgctgcca atggatggga tcagcggcgc aaagcgggaa
     4981 aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag aaccgaaaag
     5041 tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg
     5101 atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt
     5161 ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     5221 cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacggggct taaccacggg
     5281 tcaaacagcg cctcaagaat cacactttgc gtgacgtcta aagtattgat gttgatttgc
     5341 tggcggatca tcggcagcgc acagaccagc ggtttcagtt tttgataaag cccggcgtcc
     5401 attccctgta ccacgcgcat ctcactgata tcagccagcg gttgattggc ggcgtagaac
     5461 ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgtgtctg cacgctgcgg
     5521 tcctcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     5581 tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc
     5641 ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt
     5701 tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc acggctgtgc cagatgcacg
     5761 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc cgcactggcg
     5821 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     5881 attcgcccgg aaagcgtgat ggttaccagc atcataaccg ccagcaatac cagcaccacg
     5941 accagtgcca ttccgcgttt tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca
     6001 accagatgcg ttcaatctcg ccccattgcg gcgaatgcag ggtcatgcgt accgccacag
     6061 ggatcgcctg tactgacgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac
     6121 ggagcgaatc ggccggaatc aacttttgca ttgtcggctt cacgctgcct gcggcatcgg
     6181 tcagcggcca ggctaaccgt tcgagataac cgtcatgaat gcggtaaccg acggtgagca
     6241 gattgctgcg cggcagacgc atcaacgggt taaccacacc gccgcgcaca aaacgcattc
     6301 cttcactctc agacgccagc acgccagcgc cagccagtaa agctggttca cgctggccct
     6361 gatcgcctct taccggacgc ggcatcattt gggtcagatc gtgggtcaga aaactcatcg
     6421 tttgctgcat gaggtttagt ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga
     6481 cgccgttagt cacctgctgc gccatcagtg ccagcgaggc aaaaatggca attgccacca
     6541 acatttccag taacgtgaaa ccagcgcgag tccttctcac tgttggcctc ccacggcgct
     6601 aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat
     6661 atccaccgcc tggagcagcg cgttgcctgt attcaatggg attgttcgcc agtaccagcg
     6721 gcttcctgcc atatcgctct cgccctgatg cctgaccgcc cgctgctcac gggtcatcag
     6781 gtagagcagc gcagtctggt tttcagctac ccagcccgct accgtttttt cctcaagaac
     6841 gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc gccgtggcag ccagcgcaaa
     6901 aatcgccagc acgagcatca cttccagcaa ggtaaatccg cgcttcatgg catcctctca
     6961 tcacattgat tgagggataa cgcgccatcg tgtgccagct ttaccgctca acatgcgttt
     7021 tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     7081 atctgcggcg tcttcttttt cgcctctttt tgcaactcca gttcaatatc gtgcagcgtc
     7141 aggcgacgac gttgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt
     7201 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     7261 ggcaaccact gcccctgacg acgctgcata aactgatagc caggcgggtc gattagcaca
     7321 ccgagtgtct gtccttcaat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     7381 tcctgtgccg cttttttcgc gggcggttca gaatcggtcg caaacgtctg taccacgccc
     7441 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     7501 tcaggcactg cttactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgcct
     7561 tccccgtttt cctgaccatc cgcccccagg gtataaacat caaacagtcc tttttcaccc
     7621 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     7681 ccagtacggt agttgcggga gtccgccata ttggctggct gctggatcag cgcttccagc
     7741 ccctgttctg tggtcggata acgcccgtta tccagtcggt acatatctag tgcgttttcc
     7801 agcgccacga tatcgctgat ggctttttgc cgattggctt tctctttgtt gcccaacagg
     7861 ttaggcacca ccagacttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     7921 agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg aattcatgtg ataactccgt
     7981 aaattacatt ccaaccatat tattcagttg cagcatcggc tcgaggatgg cgatgacaat
     8041 aaacagcacc acgcccgcca tcatcaccac cagcgcaggc tcaaacagcc ccaacgccag
     8101 ccccacctgg gtatcaaatt cccgttcctg gttaacagcg gcctgctcaa gcatggtttc
     8161 cagttcgccg ctctgttcgc cggaagcgat aatgtacagc atcatcggcg ggaacaggcg
     8221 caactccgcc agcgcggcgc gcagactgct tccttcgcgg acgcgatctg ccgccagcag
     8281 cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa tgccttccag
     8341 tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     8401 cccgcgcgca acacggcccg tcacgggcag acgcagcaag gttttatccc agcgcaggcg
     8461 catagtagga tttttaagta gccattgccc cagcaccaga agcgccagca atcccgccag
     8521 ccagtaaacg ccgctggcct gtaacgcatc actcatagcg ataagcgcac gggtggagga
     8581 gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca ctgccgtcag
     8641 caaaatagtg accacgcccg ttgccaccac cagcataacc agcggataga gcatggcctg
     8701 caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa tctgccaggc gattgagcac
     8761 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggaatcaaa
     8821 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     8881 cagcgccatt ccgaggctct tcacatgcag tttttcactt tgctcactga ccgcctgcaa
     8941 gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc agttggcgcg tgaacagcgc
     9001 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     9061 cgatgcattc atcctggctt caatgtgcac ggggataagc tctttaccgc gcaacaactg
     9121 gcgggcatga cgcgcggaat ccgcctcaat catgcctttg gttttgcgac cattacgctc
     9181 cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg
     9241 agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatacc cgcagagtgt
     9301 tgccggacat aacgttccag ctccagctcc ccggcctgac ggtggatcaa atcacgcaat
     9361 gtggcatcca ccacgtcagc tcatggatgg cagtccgtcc gcggaaacct ttgtgattac
     9421 aggcggggca gccctgcgga tggtacagag tgacggttcg cgcatcggta atgcccagca
     9481 ggcgtttttc ttcgtcagtg gcaggcgcgg cctgacggca gtcgggacac agcgtgcgga
     9541 ccagtcgctg cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca
     9601 tatcctgcaa acgcgtgatc gcccccaccg ccgtgttggt atgcagcgtg gaaaggacca
     9661 ggtgtccggt aagcgatgcc tgaacagcga tttctgcggt ttcggtatcg cggatttcac
     9721 cgaccatcac cacatccggg tcctgacgca aaatcgcgcg caggccacgg gcgaaggtca
     9781 tgccgacgcg ggtgttaacc tgtgtctgac cgatcccttc aatcatgtat tcgatggggt
     9841 cttcaaccgt gagaatgtta cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg
     9901 tggtgctttt gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta
     9961 acagctggcg caactgcacg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc
    10021 gggcctggtt tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg
    10081 tggagacacg cacgtcaatc gcccggccgc ccagcagcag cgcaatacgt ccatcctgcg
    10141 gtacgcgctt ttcggcgata tccagccgtg ccatcacctt gatacgcgat accagcagcg
    10201 aggccagttt gcgccccggg cgcagcattt catgtaatgt gccgtcaacg cgaaaacgga
    10261 tcaccagact cttttcaaac gtctcgatgt ggatatccga agcgccttct ttaatcgcct
    10321 ctgccagcat ggcgttgatc agtttgatga tcggcgcatc gtcgtcactt tccagcagat
    10381 cttccgtttc tggcagttct tccgcgagag taaaaaagtc ctcagcagaa ccgagatctt
    10441 ccatcagctg gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa
    10501 attcggcctc gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct
    10561 gcaaaacaga gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg
    10621 ccaccaggct aaaccgacgg ctgaaactgt agggcagacg cacggtcttg gcggtggttt
    10681 cctgtgctac aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg
    10741 cggtaaggcc tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa
    10801 accttgctca tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac
    10861 gccgtctgcc gccataccgt catgcagaat ggtcgggcgg ataaacacca tcaggttacg
    10921 tttttctttt ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag
    10981 cagaggcact ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat
    11041 cagctcacca tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc
    11101 gaggctggtc tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt
    11161 accttcgtta atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt
    11221 attgaaagga ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtccta
    11281 gcccaccatg aagaaggctt cctggttgtc cagcgtagtg atgctcggcg tggagagaac
    11341 gttcgagctg gagtcgtttt taaccgcctg caccagtgcc atccagtcgc ctttcaccac
    11401 gccaaccgcc gtaccgctaa atccagaaag aagctgggcg agtgtggaga gatcgccgtt
    11461 ggtatccgga ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg
    11521 tttcgcctga gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa
    11581 ctgcattaat ccggcatctt tcgacgccca ctgcacgccg aagttgatat tgctaccttc
    11641 ggcaacttcc acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc
    11701 aattacgctt tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact
    11761 gtgtttgctg gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc
    11821 ttctttagcc gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc
    11881 tttgctgtat ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag
    11941 ccgacggatc aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact
    12001 gttggtgcgt tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct
    12061 gtttttcgtc aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg
    12121 gatcacctct tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg
    12181 ctccacaacg gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac
    12241 gttgcctgag cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga
    12301 cacattacgt accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc
    12361 ttcaccgacc agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac
    12421 gtcgttttcc atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg
    12481 gtaatactgg cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc
    12541 cggccccata atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag
    12601 gtcggtatct ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca
    12661 taatgccgct gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt
    12721 cttacgccag accgacaacg taatatcacg ccaaaacacg atgaatgatt ctcctggatt
    12781 aaatgcggtt agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta
    12841 agttgaatgg aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca
    12901 gtgaaatcct gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg
    12961 aacagagaac gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca
    13021 ggcgtaagct ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg
    13081 gcagtcggta tctcaactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg
    13141 tcactgacag cttttttgtt ggtcgcggca acggtggaac gctcctcttc agccaggctc
    13201 aggcgctcta tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc
    13261 accgcgttgt gcgagccaag cgtttcaccc tgcaaataga cctgctgttt accgccttct
    13321 tcaataaccg cgccgggtct ggcaccaaag gcgatcccgc gcgcaccaca ttaagacgcg
    13381 tttctgccac aggcacaggt tcgggttgtt ttaccggcgt ggcgacgggc tgatatttgc
    13441 caaaccagtt ttgctggctg attaattgca cgtcattttt atcgaacgtt tttgcatccg
    13501 cacggggcgg tttattcgcc gatggggaaa cagccgtata ttccgcagaa aaggagaaat
    13561 agcgccagag tgaatgcgcc acttttgcag aaataataag cagcatcagc cagaacatcc
    13621 cgcgtacaat ttttggtaat gactccaact tactgatagt
//
LOCUS       DAJVTY010000110        13469 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVTY010000110 DAJVTY010000000
VERSION     DAJVTY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13469
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.110"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(102..3323)
                     /gene="carB"
                     /locus_tag="OQQ66_002846"
     CDS             complement(102..3323)
                     /gene="carB"
                     /locus_tag="OQQ66_002846"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1905706.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OQQ66_002847"
     CDS             complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OQQ66_002847"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1905707.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OQQ66_002848"
     CDS             complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OQQ66_002848"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1905708.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OQQ66_002849"
     CDS             complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OQQ66_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1905709.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OQQ66_002850"
                     /gene_synonym="lytB"
     CDS             complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OQQ66_002850"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1905710.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVQEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OQQ66_002851"
     CDS             complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OQQ66_002851"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1905711.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OQQ66_002852"
     CDS             complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OQQ66_002852"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1905712.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRLKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OQQ66_002853"
     CDS             complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OQQ66_002853"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1905713.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHFSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OQQ66_002854"
     CDS             complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OQQ66_002854"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1905714.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12684..12902)
                     /locus_tag="OQQ66_002855"
     CDS             complement(12684..12902)
                     /locus_tag="OQQ66_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1905715.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13005..13268
                     /gene="rpsT"
                     /locus_tag="OQQ66_002856"
     CDS             13005..13268
                     /gene="rpsT"
                     /locus_tag="OQQ66_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1905716.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3145 a         3804 c         3400 g         3120 t
ORIGIN      
        1 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
       61 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
      121 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
      181 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
      241 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
      301 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
      361 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
      421 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
      481 gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
      541 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
      601 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
      661 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
      721 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta
      781 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
      841 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
      901 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
      961 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     1021 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     1081 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     1141 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     1201 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     1261 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     1321 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     1381 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     1441 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     1501 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     1561 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     1621 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     1681 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     1741 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     1801 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     1861 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     1921 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     1981 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
     2041 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
     2101 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
     2161 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
     2221 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
     2281 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
     2341 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
     2401 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
     2461 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
     2521 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
     2581 atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
     2641 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
     2701 tcatcaatca gcagctcttt ggttggggag agatccagac cgcgggcgca aatttcttca
     2761 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
     2821 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
     2881 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
     2941 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
     3001 aacacgccct gacgctccag ctccagagcg cagttcagcg ccgtctgacc gcccatcgtt
     3061 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
     3121 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
     3181 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
     3241 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
     3301 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
     3361 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
     3421 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
     3481 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
     3541 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
     3601 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
     3661 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
     3721 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
     3781 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
     3841 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
     3901 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
     3961 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
     4021 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
     4081 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
     4141 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
     4201 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
     4261 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
     4321 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
     4381 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
     4441 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
     4501 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
     4561 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
     4621 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
     4681 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcacagcaa gaagattgca
     4741 tgaaataaaa gggagcaggg aaaagtggac caaatggtca aattttacat taaaacaaca
     4801 aaaacagaag acaaaacatg taatttttgg cacttataga caatttaaaa acataacact
     4861 aaaaataaaa gggccaaaat taaagcccct tataatcaat aaattaaatg tgttattttt
     4921 gcaccataac aaatattttg tggttacaaa ctattgagat caagcacatc tcgcatatca
     4981 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
     5041 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
     5101 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
     5161 acacgttcac cggtgtggct ttcacgactg tagaccgcgc aatctttcag atctttatca
     5221 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
     5281 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
     5341 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
     5401 gcaatatcgg cggcagcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
     5461 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
     5521 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
     5581 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
     5641 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
     5701 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
     5761 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
     5821 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
     5881 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
     5941 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
     6001 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
     6061 aaattcgccc tgagtttcca ctgccacaaa acacggtttg agagtgaaca attccgggcg
     6121 caccagccag gcaatagcgc aaagatcgtg catacgcagt ccactttgca tactgccgct
     6181 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
     6241 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc tcatgacgat
     6301 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
     6361 ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
     6421 atacggttta cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
     6481 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
     6541 tggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
     6601 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
     6661 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcgttgcggg tggttttctc
     6721 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
     6781 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
     6841 atgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
     6901 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
     6961 acaatgtttt cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa
     7021 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
     7081 catttaacct cttgcaccca ctcttcctgg atgtctttcg catcgtcaat caaaaacgcg
     7141 cgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
     7201 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
     7261 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
     7321 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
     7381 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac
     7441 attccccctt ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg
     7501 tgaccgatga gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact
     7561 ttggtcacca gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta
     7621 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
     7681 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
     7741 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
     7801 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
     7861 atgttacgcc tccaatgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
     7921 cccggccaga ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
     7981 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
     8041 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
     8101 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
     8161 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
     8221 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
     8281 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
     8341 tttgccggat gcggcgtaaa cgccttatcc ggcagggttt attattgttt tttcgctcta
     8401 gaaggcaaaa aaccttccag cacaatcagt gccgcaccga cacagatggc agtatcggca
     8461 aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag
     8521 ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg
     8581 atattgttta gcttctgcgt ggccttcaag cgatacatca tcactgccag gatcacgcta
     8641 ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa
     8701 ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc
     8761 gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc
     8821 acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg
     8881 caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg
     8941 cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac
     9001 cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag
     9061 caggtgcgtc gtgatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt
     9121 catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg
     9181 cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt
     9241 cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt catcgcttcg ctgtctgcca
     9301 ggccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca
     9361 ggtagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg
     9421 cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacactg tccgctttgg
     9481 cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga
     9541 agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt
     9601 cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg
     9661 gtttcaccat atcttttgct ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg
     9721 tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca
     9781 tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt
     9841 tcatcacatc ctgcggcgaa acggtattgc cgatggattt agacatcttg cggccctgac
     9901 catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg
     9961 tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca
    10021 tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaaaag tgggtagatc
    10081 cggagtcaaa ccatacatcc aatgtgtccg gcactttcac gtactgatca gcttcatcgc
    10141 cgaggatctc tttcgcatca agatcccacc acgcctggat gccgtcaact tcaacgcgtt
    10201 ttgccacttc ttccattagt tcgagagtac gcggatgcag ctcttccgtg tctttgtgca
    10261 cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt
    10321 tagcaaccat cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga
    10381 tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg
    10441 tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt
    10501 tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt
    10561 tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc
    10621 caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt
    10681 ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga
    10741 ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcaactca agctccgcac
    10801 cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa
    10861 ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat
    10921 caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca
    10981 ccagcgagat tgggccgtta acgttgctta cggcaaattt tgctttcagt gcatcctgat
    11041 cgaccgcctg aaaagcaacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg
    11101 cttccgccag cgcagaacgg cagtcaacgc accagtgtac cggcttcgcg cctttgtgca
    11161 ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt
    11221 ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc
    11281 gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg
    11341 tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt
    11401 ggcagtccca gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga
    11461 taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag
    11521 gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt
    11581 acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac
    11641 gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct
    11701 cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa
    11761 attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa
    11821 atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt
    11881 ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc
    11941 gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctacca
    12001 cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac
    12061 ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc
    12121 ccagtaaact ttctgccaga gccagattgt catccgccag cgcctgacgc acagcggtgc
    12181 tgctaatacg cacgccacct tcgcaaaaag tttgcgtact agtgatatcg aagccgtatt
    12241 ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat
    12301 catcccctac ggcaagaaat tttacgcgca aatgcttcac cagaagatcg ctgatgaaat
    12361 tttgcgcggt taacgccgcg aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc
    12421 cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat
    12481 cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc
    12541 gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt
    12601 cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc
    12661 cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca
    12721 gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc
    12781 atggcgcaaa tcacgggaag aaagtgaccg cctgctgcaa tttttatcgc ggaaaagctg
    12841 tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac
    12901 attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct
    12961 cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat
    13021 cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct
    13081 ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg
    13141 ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc
    13201 tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac
    13261 tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta
    13321 tgcctgctgc ttttatgatg cgtgagagaa cacactaccg gttgccagat agtcaaaaga
    13381 tgctggcttt tgcagacacg ccgttacact ttctgaactc ttcgtggctt aagaatcgta
    13441 gaaccatcgg ctgatacatt ctgaacaag
//
LOCUS       DAJVTY010000111        13431 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVTY010000111 DAJVTY010000000
VERSION     DAJVTY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.111"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..309
                     /locus_tag="OQQ66_002857"
     CDS             <1..309
                     /locus_tag="OQQ66_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1905717.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OQQ66_002858"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OQQ66_002858"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1905718.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQQ66_002859"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQQ66_002859"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1905719.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3391
                     /gene="ilvY"
                     /locus_tag="OQQ66_002860"
     CDS             2501..3391
                     /gene="ilvY"
                     /locus_tag="OQQ66_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1905720.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="OQQ66_002861"
     CDS             complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="OQQ66_002861"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1905721.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="OQQ66_002862"
     CDS             complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="OQQ66_002862"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1905722.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="OQQ66_002863"
     CDS             complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="OQQ66_002863"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1905723.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="OQQ66_002864"
     CDS             complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="OQQ66_002864"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1905724.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8059..9705)
                     /locus_tag="OQQ66_002865"
     CDS             complement(8059..9705)
                     /locus_tag="OQQ66_002865"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1905725.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9708..9758)
                     /locus_tag="OQQ66_002866"
     CDS             complement(9708..9758)
                     /locus_tag="OQQ66_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905726.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="OQQ66_002867"
     CDS             complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="OQQ66_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1905727.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10295..11815
                     /locus_tag="OQQ66_002868"
                     /pseudo
     CDS             10295..11815
                     /locus_tag="OQQ66_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="OQQ66_002869"
     CDS             complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="OQQ66_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1905728.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12297..13136
                     /gene="hdfR"
                     /locus_tag="OQQ66_002870"
     CDS             12297..13136
                     /gene="hdfR"
                     /locus_tag="OQQ66_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1905729.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQSARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13232..13307)
                     /locus_tag="OQQ66_002871"
     tRNA            complement(13232..13307)
                     /locus_tag="OQQ66_002871"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13272..13274),aa:Trp,seq:cca)
     gene            complement(13316..13392)
                     /locus_tag="OQQ66_002872"
     tRNA            complement(13316..13392)
                     /locus_tag="OQQ66_002872"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13356..13358),aa:Asp,seq:gtc)
BASE COUNT         3116 a         3638 c         3479 g         3198 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 ggcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc
      181 agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catattgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc ttgtccggta
      721 ctggagccga ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag
     1081 aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac
     1201 tcttcaatga tgccggaatc gaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acgtcggagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca aatccgcctg cgggatcagt tcttcgtaag tgcccacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacattgag ggctgctcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtgacgct gactgaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca gttgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 accgattctc gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac taaccactgg
     2881 tgatgcggca gatgcgatgg aaaaggtagt cactggcgaa gcggatctgg cgattgccgg
     2941 gaaaccggag actctgcccg gcgcagtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcg cccgcgctgc cctgcccggt gcgtaaccag gtttcgtcag agaagccgga
     3061 ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg aagtagtgct
     3241 ggaaaacagc cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgaaaa
     3301 aacgccgttt gaactgggcg tttgtgcgca gaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aagattttgc cgaaccatta acccgccaaa aagaacctga acgccggatt
     3421 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3481 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3541 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3601 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3661 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3721 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3781 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3841 gaccgctcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3901 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     3961 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
     4021 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
     4081 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4141 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
     4201 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
     4261 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4321 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4381 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4441 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4501 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4561 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4621 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4681 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4741 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4801 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgctg cctcgtaaac
     4861 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccag acaggggttg
     4921 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     4981 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5041 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5101 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5161 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5221 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5281 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5341 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5401 accgcgtcgt cctggctttc gtacactttt gccgggccgg tgaatttgag gatgctgtca
     5461 tcgacgcccg cggttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5521 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5581 tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg gatacccgca
     5641 gggccggcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgc
     5701 tccagcgttt gcggcaacgt caggccgagc acgtttttca catcacggtt cagtaacccc
     5761 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
     5821 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
     5881 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     5941 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgcc
     6001 ttactggcga tattacgcgg cagtgcactt tcgtcgtttt gctcgtaata acgtttggtc
     6061 aattcaacaa tgcgtttacc agcattaagg aacagctgct tacggtccgc gtgggttgcc
     6121 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6181 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6241 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6301 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
     6361 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6421 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6481 tcagcgatca gttcgcgaga tggaagtgaa taaagcatcc ccccgtggcc catggcaatc
     6541 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6601 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6661 gagttcacaa ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc
     6721 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6781 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
     6841 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6901 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
     6961 aacctgaata ccgtctacgc tgcgcactgg cgtgatttct gccgcagtac cggacataaa
     7021 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7081 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7141 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7201 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7261 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7321 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7381 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7441 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7501 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7561 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7621 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7681 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7741 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7801 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7861 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7921 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     7981 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
     8041 cattgacctg atattgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8101 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8161 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8221 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8281 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8341 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8401 cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
     8461 gacttgtgcg ccaaccgccg ctggtaaacc aaaacccata gtgcctaagc cgctggaggt
     8521 gatgaaattt tccgggcgag tgtgggcgat gtgttgcgca gcccacatct ggtgctgccc
     8581 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8641 cggcgcgtag atagcgtcgc cgggatggtc gtaacgccag gcatgttcat cacgcagctg
     8701 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaattctg gtaacagagc
     8761 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8821 gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
     8881 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
     8941 cgctttggtg ccatgcattc ccagcatgcc cagatagtac ggataatctg cttcaactgc
     9001 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
     9061 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
     9121 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9181 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
     9241 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccataatgcg
     9301 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9361 caggacatcc acttcctgaa atgcatcagt gccgataaac ggtgcggaca cttgaccggt
     9421 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9481 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9541 gatagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
     9601 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9661 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtg
     9721 ctgaatgttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9781 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9841 atttctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9901 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
     9961 cttgttcgaa ggaatgccta aagagttacc ataggttttg taaatagtac aagatatttt
    10021 ttggccgatt tttatgatgc gtttaataat ttcaatataa gttgttgttt tttaaaaata
    10081 aaatagtgag taaaaaaatt caatttcagt atgaatgttg ttcccttcct cgtagttcat
    10141 cctttgtccg ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt
    10201 gcacaaataa acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata
    10261 tcttatgcac ataatggtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg
    10321 cgcagccctg ggagtaaatg cgcccccgat cactgttgag gtacatatca gtaaaggtct
    10381 acccggctta acgaaggtgg gcttaccaga aacaacggta aaagaagctc gcgatcgcgt
    10441 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10501 ggctccagct gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10561 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatggat tagtcggaga
    10621 actggcgctt acaggcgctc tgcgtggcgt tcccggcgca atctccagta caactgaagc
    10681 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10741 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10801 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10861 tctcagtgat gttatcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10921 cgggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    10981 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagtg ctgcgatatt
    11041 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11101 tcatcacagt gcatcgttaa ctgcgatggt aggcggtggc gcaattccag ggcccggtga
    11161 aatttcgctg gcgcataacg gcgtgctttt tcttgatgag ttacctgaat ttgaaaggcg
    11221 tacactggat gccttgcgag agccgattga atccgggcag atccatcttt cacgcacgcg
    11281 agcaaaaata acctatccag cccgtttcca gcttgtcgcg gcgatgaatc ccagccctac
    11341 cggacattat cagggaaacc ataaccgctg cacaccagag caaacgctgc gttatctcaa
    11401 ccgactctct ggtccctttc tcgaccgctt cgatctctca ctggagatcc cattaccgcc
    11461 tcccggtatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    11521 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaacg cctggctgga
    11581 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggctggaaga
    11641 aacgctgatc catctggggt tatcgattcg tgcctagcag cggttattga aagttgcacg
    11701 aaccattgct gatattgatc agtctgacat tatcacacgt caacatttgc aggaggcagt
    11761 tagctatcga gcgattgacc gtttgctcat ccatctgcag aaactactga cataaaaaaa
    11821 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    11881 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    11941 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    12001 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    12061 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    12121 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    12181 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    12241 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    12301 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    12361 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    12421 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    12481 aaaaactact gccttatgca gaaacgctca tgagcacctg gcagtccgcc cgcaaggagg
    12541 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat
    12601 gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac
    12661 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    12721 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    12781 gatatttcac tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt
    12841 atctgcgact tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    12901 acgaagtgcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    12961 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    13021 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    13081 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg
    13141 aatggctggc aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa
    13201 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    13261 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    13321 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    13381 aactaccgct ccaccgaatt cttttacaac caccggtttt atgaccggct t
//
LOCUS       DAJVTY010000112        13408 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVTY010000112 DAJVTY010000000
VERSION     DAJVTY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.112"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(72..1445)
                     /gene="cobA"
                     /locus_tag="OQQ66_002873"
     CDS             complement(72..1445)
                     /gene="cobA"
                     /locus_tag="OQQ66_002873"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1905730.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1464..2270)
                     /gene="nirC"
                     /locus_tag="OQQ66_002874"
     CDS             complement(1464..2270)
                     /gene="nirC"
                     /locus_tag="OQQ66_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1905731.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2397..2723)
                     /gene="nirD"
                     /locus_tag="OQQ66_002875"
     CDS             complement(2397..2723)
                     /gene="nirD"
                     /locus_tag="OQQ66_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1905732.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2720..5263)
                     /gene="nirB"
                     /locus_tag="OQQ66_002876"
     CDS             complement(2720..5263)
                     /gene="nirB"
                     /locus_tag="OQQ66_002876"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1905733.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5525..6706)
                     /gene="tsgA"
                     /locus_tag="OQQ66_002877"
     CDS             complement(5525..6706)
                     /gene="tsgA"
                     /locus_tag="OQQ66_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1905734.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            6977..7549
                     /gene="ppiA"
                     /locus_tag="OQQ66_002878"
                     /gene_synonym="rotA"
     CDS             6977..7549
                     /gene="ppiA"
                     /locus_tag="OQQ66_002878"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1905735.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7654..7821
                     /locus_tag="OQQ66_002879"
     CDS             7654..7821
                     /locus_tag="OQQ66_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1905736.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7811..8413
                     /locus_tag="OQQ66_002880"
     CDS             7811..8413
                     /locus_tag="OQQ66_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1905737.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8445..9008
                     /gene="pabA"
                     /locus_tag="OQQ66_002881"
     CDS             8445..9008
                     /gene="pabA"
                     /locus_tag="OQQ66_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1905738.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9094..10314
                     /gene="argD/dapC"
                     /locus_tag="OQQ66_002882"
     CDS             9094..10314
                     /gene="argD/dapC"
                     /locus_tag="OQQ66_002882"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1905739.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10381..12471)
                     /locus_tag="OQQ66_002883"
     CDS             complement(10381..12471)
                     /locus_tag="OQQ66_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1905740.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12522..13154)
                     /gene="crp"
                     /locus_tag="OQQ66_002884"
     CDS             complement(12522..13154)
                     /gene="crp"
                     /locus_tag="OQQ66_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1905741.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3228 a         3472 c         3524 g         3184 t
ORIGIN      
        1 ggttatgttt tatttgctga atatataaaa agagtcatta agaaggccag aattctctgg
       61 cccgggttaa attaatggtt ggagaaccag ttcagtttat cgcgcaggcc aacaacccga
      121 ccaataataa ttagcgatgg actgttcatt tgctgagcaa gttcaccgag ttgcgtgagc
      181 gtaccgtcaa tcacgcgctg cgtgactgcc gttccgtttt cgacaattgc caccggcatt
      241 tcgccaggca taccgtgttc aatcagcttt tgctgaatag tcgcggcctg attcaacccc
      301 atatagaaca ccagcgtctg tttttctgcc gccaggtttt cccagtccag ctcgccaccg
      361 gtttttaagt gtccggtaat taagcgtacg ctctgggcat aatcgcgatg cgtgagtgga
      421 atacccgaat aggcagagca accagaagct gcggtaatac ccggaaccac cgagaacgga
      481 atacccgcgt tgcacagtgt ttccagctct tcgccaccac ggccaaaaat aaacggatcg
      541 ccacctttca gccgcaccac gcgtttgcct ttttgcgctt cccgcagcag gatctggtta
      601 atctcttcct gcggtacaca gtggtatccc gcgcgtttgc cgacgaaaac gcgatcagca
      661 tcgcggcgta ccagattcat aatatcgtca gaaaccagac ggtcgtagac caccacatct
      721 gcctgctgaa tttgttgcag tcctttcagc gtcagcagcc cggcatcgcc cggacctgca
      781 ccaaccagca ccacttcacc gcgatggtcg agcggttcgt tgattaactg ttcggtcgtt
      841 tcagtaatgg ctttctgatc gttgtttgcc agcgactgcg ccaggcggtc gttaacgaac
      901 aatttctccc agaaacggcg acgctcactc atcgtggtga actgttgttt cactcgcccg
      961 cgtaattgcc cggcatattt cgccacctgg cccagatgca acggcagcag tgattcgagt
     1021 ttttcgcgca acaggcgcgc cagaaccgga gaggtgccgc cagaggagac cgctaccatt
     1081 agcggtgagc ggtcaataat cgacggcata ataaagctgg cggctttcgg cgcatcgact
     1141 acgttacaga agatgcgacg agcttcagcg gcttcgctga cgcgctggtt aagcgtgtca
     1201 tcatccgtcg ctgcaatcgc cagccagcag gtgtcgagaa ggctttcatc aaatggccct
     1261 tcgacgaggg ttaacatgcc tgcatctgcc catgcggtga actgtggaat aaacgctaat
     1321 gcattcaccg ttaagcgagc gcctgcgtct aacagcaacc ttgctttgcg ttccgcgaca
     1381 tcaccaccgc cgacaatcag acagtcgcga tcgcgtaatt ggcaaaatat aggcaaatga
     1441 tccacgtaaa aaccccttag taattaaccg gcagccgttt cagtttgatt aaatttgtcc
     1501 gcaaccggac gattcgattt cggcgtagca taccaataac ccaatcccat gaatacggca
     1561 cctgataaag tattacccag cgtcacccac agcaggttat gaccaatacc cgccagcgtg
     1621 taggcttcgc tgtggttgcc gaaccaggag agcgcgaaca gcgtcatgtt agcgatagag
     1681 tgctcgtagc cggacgcgat aaatgccagc agacaccacc agatagcgat aaatttcgcc
     1741 gccccttcag tgcgcagcgc catccagatt gccaggcaaa ccagccagtt acacaatgca
     1801 cctttgaaga agagtaccat tgccggtgca gtggttttag ccagcgcgac ggagtgaacg
     1861 atgctggtat ctaccggcag caggctaccg ccgccccagc tatagagcat ggcaacgaag
     1921 acggaaccga ccaggttacc cagccaggtt tgcggcagga ttgcccacat ttgcccgtgg
     1981 ctgatgctgc ccgcttttac cccaaaggtg aggaacatgg tgtgtccggt gaacagttca
     2041 gaaccggcga taatcaccag cgttaaggcg ataccaaagg tcgcgcccat caccagcgga
     2101 cgtacggacg ggtcgagcaa attaccgagc gtgaaaatca agatgatccc aagacccaca
     2161 tacgcgcccg ccatggcgga gctgacccaa aagccgagcg ggttatttgc cgacaggcgt
     2221 gcaatgcgcg cagcgttagc cgcacactta ttaatagtgt ctgtaaacat ttgattatcc
     2281 tttaaaaaat aaaaacaaaa aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc
     2341 gaaggtaatt acaggaccat gcaaaaaggg gaggcattgc gcctcccgtt aaaacattaa
     2401 ccgcgcagct gcaccacgcc gtctttcact cgtgcttcgt aatgtttgac ggaaaactgt
     2461 tcgtcttcca tgcacaagcc atcacttaag cgaaaacgct gttttttcag cgggctggcg
     2521 acccacagct cgccctggtg ttccgcaatc agcccgcgtg acagcacgct ggactcgaag
     2581 aacggatcga tgttgctgat cgcaaacacc tggtcgctgt gatacgggcg gaaaatcgcg
     2641 acctgctcgt cgcctaacag cgcgcagacg ccggtttcag gcaggatgtc atcgattttg
     2701 cagatgtctt tccactggct catgcgttgt cctccaccag agttaccggg atacgttcat
     2761 acggcgttgc cggacggtgc tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct
     2821 tgtcgctgtt gatgaagtgt ttgaagcgag tctgcgcgga cggtgtattg accgtttcag
     2881 tccactcaca cactaccgct tcacgcaggc gcgccatctc ttcttccaga tgagcgttca
     2941 gccccagttt gtcgtcaatg atcaccgctt tcaggtaatc gatgccgcct tcgaggtttt
     3001 ctaaccacgg cgcggtacgc gtcagtttgt cggcggtacg gatgtagaac atcatgaagc
     3061 ggtcgagata tttgatcagc gtttcgcgat cgatatcagc tgccagcaga tccgcatgac
     3121 gcggtttcat gccgccgttg ccgcaaacat acaggttcca gcctttttca gtggcgataa
     3181 tacccacgtc tttaccctga gcttctgaac attcacgggt acagccggag acaccgaact
     3241 tcattttgtg cggcgtacgg atgcctttgt agcggttttc cagttccacg ccgaggccga
     3301 cgctgtcgcc aacgccgtag cggcaccagg tgctacccac gcaggttttc gccatacgca
     3361 gtgctttcgc ataggcatga ccggtttcga agccggcttc aatcagctga cgccagatct
     3421 ctggcagatc gtctttctgt gcgccaaaca tcgccaggcg ctgggaaccg gtgattttgg
     3481 tgtagagatt aaattcacgc gcgatacgac ccactgccat cagcccttcc ggggtgattt
     3541 caccgcccgg agaacgcggg atcaccgagt aggtcccgtc tttctggatg ttagcgagga
     3601 agttgtcgtt agaatcttgc agcggagtat gttctggctt cagaatgtat tcgttccagc
     3661 aggaggccag cagcgaaccg acggtcggtt tacaaacttc acaaccgtag cctttgccgt
     3721 gtttcgccag cagttcttcg aaggttttaa tgccttcaac gcggatcaaa tggaacagtt
     3781 cctgacgcga ataagcaaag tgttcgcaca ggttgttgtt aacttcgatg ccctgtttcg
     3841 ccagttccgc gttcagtacc tgagtgacca gcgggataca gccaccgcag ccagtacccg
     3901 ctttggtttc agctttcagc gccgcaactg tgtggcagcc tttgttgatg gcagcaatca
     3961 gatcaccttt agtgacgtcg aagcaggagc agatttgcgc gctgtccggc agtttatcaa
     4021 caccgataga cggcttaccg ctacccgagt gcgccggcag aatcagagaa tccgggtttt
     4081 ccggcagctc gatagcattc agcaccagtt gcagcaagtt accgtagtcg ctggtatcgc
     4141 ccaccagtac cgcaccgagc agggttttgt tgtcttcgct gacgatcagg cgtttgtaga
     4201 tctctttgct ttcgtcgagg taaacgtagc tacgtgcgcc aggcgtgcga ccatgagcat
     4261 caccaatacc gcctacgtcc acgcccagca gtttcagctt ggcgctaagg tcagcacctt
     4321 caaaggcgtt ttcgctaccg agaatatggt caacggcgac ctgcgccatt ttgtagccag
     4381 gtgctaccag accaaatata cggttgttcc agcttgcgca ttcaccgata gcgtagatat
     4441 ccggatcgga agtttggcag gaatcattaa tgacgatacc cccacgcgga gcaacgtcca
     4501 gaccacactg ggttgccagc ttatcgcgcg gacggatacc ggtagagaag acgataaagt
     4561 cgacttccag ttcgctgccg tcggcaaaac gcatggtttt acgtgcttcg acaccttcct
     4621 gcacaatctc aagggtgttt ttgctggtgt gaacgcgcac gcccatactt tcgattttgc
     4681 gacgcagctg ctcgccaccc atctgatcaa gctgttccgc catcagcata ggggcaaatt
     4741 cgataacgtg ggtttcaata cctaagtttt tcagcgcgcc agcggcttcc agacctaaca
     4801 ggccgccacc aacaacggca ccgcgtttgc tgcgacgggc gcaggattca atggcgttga
     4861 ggtcttcaat ggtgcgatag acaaagcagt cctgagtatc agaacctttg attggtggga
     4921 tccacgggta ggaaccggtt gccatgatca gcttgtcata aaaaacggta cgtccggcgc
     4981 tggagtgaat caccttctcc tgacggttga tggtgatagc gcgttcgccg accagaactt
     5041 tgatgccgtg tttctcgtag aagccttcgc gcaccagcga cagctcttcg gcggtgtggt
     5101 gagagaagta agacgagagg tgtacgcggt cataagcgat gcgcggttct tcacagaaaa
     5161 cggtaatatc aaagttggcc gcatcagatt tatcaagaag atcttcgata aagcgatggc
     5221 cgaccatacc gttaccgata attgcgagtc tgactttgct catttttgcc tcgatttctt
     5281 ttctattacc gcctacctta acgattcagc aaccccgctt attgatgtaa atcaaattca
     5341 cctttatata ccccttaagg ggtatattgc tgatttgtat aaattttctt aagtcacgga
     5401 attgtctgac atttcatgtt tgtgcaaaaa atagacaaaa tgaacgggtt tatagaaata
     5461 gatgcacgta gcggggttag agaggaaaag gccggacgac gatagtgccg tccggcatta
     5521 gcaattaatg agaggtcagg gtgttatgct gacggtgacg gctgacgaaa cctaacagga
     5581 agcacatcac aaagacgaca gcgtacagac cgtttgctgt aagcagtgcc gcctgcggac
     5641 cgctatgttc aacaatcggg ccggtaacca caaaggtcaa catagtaccg atggttccgc
     5701 aggtcaggac aaagttaacc agttttggcg acggtacttt ggtctgctgt gaacccaggg
     5761 tgatgatggt ggtatagatc gcgctggaga agaagcccag agcgagaatt gaccacgcca
     5821 tatgtgctgg tgttccggtg ttaaagacgt acatcagaat cgcagccaga ccagccagta
     5881 cggtcagaat gcgttgcaaa tcaaagaagc gaagaataaa gctgaacgcc cacatgccga
     5941 ccatgtatga catccagaag ttactcacca gcgtgcccgc gtcgttcagg ctcatgccca
     6001 ggcctttggc atactcaggc acccaggaga taaaacctaa ctgaccgagg atgtagcaca
     6061 gcgccgcaac agagagaaac agtacgccga tcccccactt ttctttctct accggagcat
     6121 ccgtttttgg tgcatgtttg cccagtgctg ggaactcaca gccgaaggtc agaataaaaa
     6181 tagcgacata caccagcccg atgcaggcat aaacccagta ccactcaatg ctgcgcgcca
     6241 gtagaaacgc ggcgatcatt gggaaaatca tcccagccat actgaagaag gagtcagtaa
     6301 ataacaggcg ggaaccgcgc tgacgccctt catacatttg tgttatcagg aatgtaccaa
     6361 tcgacatagt aatgccgctg accaccccga gaatgaacat cgccgtcgag aacagcgcca
     6421 ggctgtggct gaacatcaaa ccggcaaccg ccagcaccat caggagaaag ccaaaacgta
     6481 actgcgtttt caacgggacg atttccatca gccaggcgtt gaggaagata gagattaaaa
     6541 tgccggcgtt gaggaaggtg aaggtgttac tcatactgga aacaggcaga ttgaaataat
     6601 cggcgatatt tcccatcacc atcccggtga caataaccaa cgcaccagtc agtgcgtagg
     6661 agagaaagct aatccatgtg agcttgatgc gattgctgtt agtcatgatt ggcctgcgtt
     6721 caaaaataaa atggcatagc gggatatgcc gcgagcgggt gattttaggt gattttgtga
     6781 tctgtttaaa tgttttattg caatcggttg ctaaattgca ttttaagaag tgatcttgat
     6841 cacggaataa aaagtgatcg tcaggttaca tatatttcag atacgtaaaa ttaggtaaag
     6901 ggatggcctt gttcttgaag gctatttaga atctcttcac ttgctttttt tctgctctgt
     6961 ttgttaagga aatctcatgt tcaaatcgac cctggcggcg atggctgctg ttttcgctct
     7021 ttctgctctt tctcccgcag caatggcagc gaaaggggcc ccgcacgtat tgttgacaac
     7081 ctcagctggt aacatcgaac tggagctgga taaacaaaaa gcgccagtgt ctgtgcaaaa
     7141 ctttgtcgat tatgtgaaca gcggttttta taacaacact acctttcacc gcgtcattcc
     7201 tggctttatg attcagggcg gcggtttcac cgagcagatg cagcagaaaa aaccaaaccc
     7261 gccaatcaaa aatgaagccg ataacggcct gcgcaacacg cgtggcacca tcgcgatggc
     7321 gcgtaccgct gacaaagata gcgccaccag ccagttcttt atcaacgttg ccgataacgc
     7381 cttccttgac catggtcagc gtgatttcgg ttacgcggta tttggtaaag tggtgaaagg
     7441 catggacgtg gccgataaga tttcccaggt gccgactcat gacgttggtc cgtaccagaa
     7501 tgtgccgtca aaaccggtag ttatcctttc cgctaaagtc ctgccgtaat gatttctcgc
     7561 gcgggcaatc ttgcccgcgc ttctgctctc ccggcgtaac ccggatttgc cgcttatact
     7621 tgtggcaaat ggacacgttc agggaggcat caagtgaaga aactcaccga taagcaaaag
     7681 tcccgtctct gggagcttca gcgtaatcgt aatttccagg ccagtcgccg tcttgaaggc
     7741 gtcgagatgc ctttagtcac tcttactgcc gcagaggctt tagcgcgcct tgtagagctg
     7801 aggaggcact atgagcgata aattcggcga agggcgcgat ccgtatcttt atccaggcct
     7861 tgatatcatg cgtaaccggc tgaacatccg ccagcaacaa cggctggaac aggccgctta
     7921 cgaaatgacg gcgctgcgtg ctgcgaccat tgagcttggt ccgctggtgc gcggtttacc
     7981 gcatttgcga actatccatc gccagctgta tcaggatatt ttcgactggg cagggcaact
     8041 gcgtgaagtt gatatttatc agggcgatac gccgttctgc cactttgctt atatagaaaa
     8101 agagggcaat gccctgatgc aggatctgga ggaagaaggt tatctggttg gcctggagaa
     8161 aacgaagttc gtcgagcggc tggcgcatta ctattgtgaa atcaacgtgc tgcatccctt
     8221 ccgggtggga agtggtctgg cacagcggat cttcttcgag caactggcga ttcatgccgg
     8281 atatcaactg agctggcagg gtatcgaaaa agaggcctgg aatcaggcaa atcagagtgg
     8341 ggcaatgggg gatctcaccg cactgcagat gatatttagc aaagtggtaa gcgaagccgg
     8401 ggaatctgag taaaatagcg cggttctttc gtaccggagc cgccatgatc ctgcttattg
     8461 ataactacga ttcttttacc tggaacctct accagtactt ttgtgaactg ggggcggatg
     8521 tgctggttaa gcgcaacgat gcgttgacgt tggcggatat cgacgccctt aaaccacaaa
     8581 aaattgtcat ctcacccggc ccctgtacgc cagatgaagc cgggatctcc cttgacgtta
     8641 ttcgccacta tgccgggcgc ttgccgattc ttggcgtctg cctcggtcat caggcaatgg
     8701 cgcaggcatt tggcggtaaa gttgtgcgcg ctgcaaaggt catgcacggc aaaacctcgc
     8761 cgattacaca taacggtgag ggcgtatttc gggggctggc aaatccactt accgtgacac
     8821 gctaccattc gctggtggtg gaacctggct cattaccagc gtgctttgac gtgacggcct
     8881 ggagcgaaac ccgcgagatt atggggattc gccatcgcca gtgggatctg gaaggtgtgc
     8941 agttccatcc agaaagtatt cttagcgaac aaggacatca actgctggct aatttcctgc
     9001 atctctgatt tccgattgcc attcagtgat tttttatgca tattttgtga ttataatttc
     9061 atatttattt atgcgtaaca gggtgatcat gagatggcaa ttgaacaaac agcaattaca
     9121 cgcgcgactt tcgatgaagt gatcctgccg atttatgctc cggcagagtt tattccggta
     9181 aaaggtcagg gcagccgaat ctgggatcag caaggcaagg agtatgtcga tttcgcgggt
     9241 ggcattgcag ttacggcgtt gggccattgc catcctgcgc tggtgaacgc gttaaaaacc
     9301 cagggcgaaa ctctgtggca tatcagtaac gttttcacca atgaaccggc gctgcgtctt
     9361 gggcgcaaac tgattgaggc gacgtttgcc gaacgcgtgg tgtttatgaa ctccggcacg
     9421 gaagctaacg aaaccgcctt taaactggca cgccattacg cctgtgtgcg tcatagcccg
     9481 ttcaaaacca aaattattgc cttccataac gcttttcatg gtcgctcgct gtttaccgtt
     9541 tcggtgggtg ggcagccaaa atattccgac ggctttgggc cgaaaccggc agacatcatc
     9601 cacgttccct ttaacgatct ccatgcagtg aaagcggtga tggatgatca cacctgtgcg
     9661 gtggtggttg agccgatcca gggcgagggc ggtgtgacgg catcgacgcc agagtttttg
     9721 cagggcttgc gcgagctgtg cgatcaacat caggcattat tggtgtttga tgaagtgcag
     9781 tgcgggatgg ggcggactgg cgatttgttt gcttacatgt actacggtgt gacgccggat
     9841 attctgactt ctgcgaaagc gttaggcggc ggcttcccga ttagcgccat gctgaccacg
     9901 gcggaaattg cttctgcgtt tcatcctggt tctcacggtt ccacctacgg cggtaatcct
     9961 ctggcctgtg cagtagcggg cgcggcgttt gatatcatca atacccctga agtgctggaa
    10021 ggcattcagg cgaaacgcca gcgttttgtt gaccatttgc agaagatcga tcagcagtac
    10081 gatgtgttta gcgatattcg cggtatgggg ctgttaattg gcgcggagct gaaaccacac
    10141 tacaaaggtc gggcgcgtga tttcctgtat gcgggtgcag aggttggcgt aatggtgctg
    10201 aatgccggac cggatgtgat gcgtttagca ccgtcgctgg tggtggaaga tgcggatatc
    10261 gatgaaggga tgcaacgttt cgcccacgcg gtggcgaagg tggttggggc gtaaataaga
    10321 cgcatcaggc aaaatatgtc ggatgcggcg tgaatgcctt atccgatggt ttagcctgta
    10381 ttacgccttc gaatcccgca acttgcgact cagccaaatc ccgtgatgcg gtcgctgacg
    10441 ccatgccatc gacgaaatgg tgtgcatggt gttcagatga ccaataaccc gctgtaaatg
    10501 ctgctccagc gtacctgccg cgccttcgtg cggctgcatc tccggcgcat ccatgatatt
    10561 ggcatcgcca gaactacccg gttcgtcata ctccagtcgc tgctgacaac gctgaatggc
    10621 gatttcacaa gactgtaaat actcttgtgc cagttcaggt ggcaatgccc ggtgttcccg
    10681 cgccagcgtg gtcatggcat taatatgctc aacaataaac tggctgtgcg ttacccacag
    10741 tttcatatct gccagataat ggctgttaaa cgccggttcc tgcatcgcct gattcaatga
    10801 gttatacaga gtgttatgtg cctgatttac ccgcattcgc tgccaggcca gtggcgtagg
    10861 ttgcggatcc tcgctaagaa tcaagcgaat cgcttcctga taggcttcta aagcgtcatg
    10921 ggcgttttta cgcaataacc cgctctgcca ctgcggccac agccagacag taccgccgaa
    10981 agcaattaaa caaccaataa tggtatcgat aagacgcgga aggatgtatt gctcgccgtt
    11041 caaccacaat agttgcaggg tatacactgc ggtaatagta aaaccgaccg tcgcccagcc
    11101 gtagtttttg cgcaatatca ggtagctggc gagggtggta atcagcatca acgtcagggt
    11161 gtaaccttcg ggaattttaa agtgcagcgc cacgcccgca atgattaacc cgaccacggt
    11221 tcccacggag cgattcacaa tcctcagacg ggttgcgcca tagccatttt gtgtcaccag
    11281 caataccgtc atcaggatcc agtacgactt cggcagatgc agcgcggtgc ccatcaggct
    11341 ggcaacgctt aacatcacac tgagtcgtcc ggcattgcgt agcgccggag actttagtga
    11401 cagataactt ttcagcgcag gaagtaatgg cattcgccgc tgtttatcgg ccagtaagtc
    11461 acgggcatag agcggttttt gggtgcgcag cacgcgggcg atgcggctga aatgccagta
    11521 gcagaattgc ccaaccggat tatccggatg ctggcgggcg attttttcca gtgcgccaat
    11581 ttgcttttcc atcgtaaaac gcgttggcag gcgatggtaa agaatgtcat cagccagcac
    11641 gcgcaggcga gcggcgacgg tttgcgcatt ccagcggata acttcttccg catggctacg
    11701 ctcgaccagc ttttgcacct cttccggctg atgcaaactg accgaaatat gttcctgtaa
    11761 atccagcgcc tcctggaaaa tacgcagcat ccgcttgtag tcagtattat tttgcgcgga
    11821 aagcatatgc atttgctgat agcactgggt aattagatcg accgcttttt gctggcgcac
    11881 cagcagcggc ggcagcgctt tttcagggtc ggtgtgctgg gtaagcaggc tgtatttggc
    11941 ttcacaataa tctgccagtt cacggtacag cagacttagt gactcgcgca gcggttgttc
    12001 gcgccagatc cagaaccaaa accagttaaa caatccgtac cagagagtgc ccaacgcata
    12061 gatgagcaac ggttcccaga ccggcatgta tcccgccaga ctgagggtaa aaatggctgc
    12121 cagcagcgat gcgggaagca attttgcgtg caatggcccc aactcagcag tgacgccaag
    12181 taccagcgtt aatccggtca gcaaaaaggg caggggaaca tcttttgcca gtagcagctg
    12241 tgtcagtaag ctacaggtgg caaacagcga cgcaccaatg attaagcgtt tgaaaaaacg
    12301 tttatgaggc gtatcaaggc ccgcaatatt gcaacaggca ggaacgagag agaagagcag
    12361 accgaatcgt aattcgccaa gcattaaccc aacggccacg ggcaaacata gcaccagcgt
    12421 ttgtcgaagt gcatagttga tatcggggtg ataaatcagt ctgcgccaca tcgggggaaa
    12481 caaaatggcg cgctaccagg taacgcgcca ctccgacggg attaacgagt gccgtaaacg
    12541 acgatggttt taccgtgtgc ggagatcagg ttctgatctt caagcatctt cagaatgcgt
    12601 cccacggttt cacgagaaca gccgacaatc tgaccgattt cctgacgggt aattttgatt
    12661 tgcataccgt ccgggtgagt catagcgtct ggttgttttg ccagattcag cagagtctgt
    12721 gcaatgcggc ccgtcacgtc gaggaacgcc aggttgccca ctttctctga agtgacttgc
    12781 agacgacgcg ccatctgtgc agacagacgc atcagaatgt ccgggtttac ctgaatcaat
    12841 tggcgaaatt ttttgtacga aatttcagcc acttcacagg cggttttcgc acgtacccat
    12901 gcgctacgtt cctggccctc ttcaaacagg cccagttcgc caataaaatc accctgattc
    12961 agataggaga ggatcatttc tttaccctct tcgtctttga tcagcactgc cacagagcct
    13021 ttaacgatgt agtacagcgt ttccgctttt tcaccctggt gaataagcgt gctcttggat
    13081 gggtacttat gaatgtggca atgagacaag aaccattcga gagtcgggtc tgtttgcggt
    13141 ttgccaagca ccatgcgcgg ttatcctctg ttataagctt tctccagagc cagataacgc
    13201 cgctgtctct ggattgccga aatatgcttc ccgctacctg ggaaggggct atcaactgta
    13261 ctgcacggta atgtgacgtc ctttgcatac atgcagtaca tcaatgtatt actgtagcat
    13321 cctgactgtt ttagcatagc tttcgctttg tgtctcctgg tgtctcgctt cagcatgacc
    13381 caggtcgcct tccgttgcgc gatttggt
//
LOCUS       DAJVTY010000113        13360 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVTY010000113 DAJVTY010000000
VERSION     DAJVTY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13360
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.113"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..174
                     /locus_tag="OQQ66_002885"
     CDS             <1..174
                     /locus_tag="OQQ66_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1905742.1"
                     /translation="AAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRRL
                     SESVPCMPHKKAG"
     gene            195..896
                     /gene="mlrA"
                     /locus_tag="OQQ66_002886"
     CDS             195..896
                     /gene="mlrA"
                     /locus_tag="OQQ66_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS1905743.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            956..1063
                     /locus_tag="OQQ66_002887"
     CDS             956..1063
                     /locus_tag="OQQ66_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1905744.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1044..1775)
                     /gene="yehW"
                     /locus_tag="OQQ66_002888"
     CDS             complement(1044..1775)
                     /gene="yehW"
                     /locus_tag="OQQ66_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1905745.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1780..2706)
                     /gene="yehX"
                     /locus_tag="OQQ66_002889"
     CDS             complement(1780..2706)
                     /gene="yehX"
                     /locus_tag="OQQ66_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1905746.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2699..3856)
                     /gene="yehY"
                     /locus_tag="OQQ66_002890"
     CDS             complement(2699..3856)
                     /gene="yehY"
                     /locus_tag="OQQ66_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1905747.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3863..4780)
                     /gene="osmF"
                     /locus_tag="OQQ66_002891"
     CDS             complement(3863..4780)
                     /gene="osmF"
                     /locus_tag="OQQ66_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1905748.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5032..7329)
                     /gene="bglX"
                     /locus_tag="OQQ66_002892"
     CDS             complement(5032..7329)
                     /gene="bglX"
                     /locus_tag="OQQ66_002892"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1905749.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7525..9240
                     /gene="dld"
                     /locus_tag="OQQ66_002893"
     CDS             7525..9240
                     /gene="dld"
                     /locus_tag="OQQ66_002893"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1905750.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9279..10211)
                     /gene="pbpG"
                     /locus_tag="OQQ66_002894"
     CDS             complement(9279..10211)
                     /gene="pbpG"
                     /locus_tag="OQQ66_002894"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1905751.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10385..10972)
                     /locus_tag="OQQ66_002895"
     CDS             complement(10385..10972)
                     /locus_tag="OQQ66_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1905752.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11142..11720
                     /locus_tag="OQQ66_002896"
     CDS             11142..11720
                     /locus_tag="OQQ66_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1905753.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11850..12611)
                     /locus_tag="OQQ66_002897"
     CDS             complement(11850..12611)
                     /locus_tag="OQQ66_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1905754.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12664..>13360)
                     /locus_tag="OQQ66_002898"
     CDS             complement(12664..>13360)
                     /locus_tag="OQQ66_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCS1905755.1"
                     /translation="NDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELG
                     YSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQ
                     MGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAE
                     KNQHQAQIERDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDAS
                     IQLTGALGGGYKR"
BASE COUNT         3209 a         3740 c         3377 g         3034 t
ORIGIN      
        1 gccgcaggag ctaatcctag ttttatcgca agccagatgg gccacacaaa cgcacaaatg
       61 gtattcaatg tttacggagc atggatgaaa gacaacaatc acgaacagat agaacttctt
      121 aacagaagac tatctgaaag tgtcccatgc atgccccata agaaagcggg gtaaaataaa
      181 aacttgcaaa atcaattggt ttacctttaa tccctgtcac gttacgcgcg tggcagaggc
      241 gttacgggtt gctgaaaccg caacggacag acggcggtca tcgactgttc aacgatgccg
      301 atattgaccg gatccgcgag atcaaacgct ggatcgacaa cggcgtgcag gtcagcaaag
      361 ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc gatcagcaag
      421 aaacattact gacctacctg caaagcggca atctgcatag cctgcgaacg tggatcaaag
      481 agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt cctctgcgcc
      541 gacggcttca gtgccaacaa ccgactctcc aggcgctgct ggcgatcctc gacggcgtac
      601 tgatcaacta catcgccatt tgtctggctt cggcacgtag aaaacagggt aaagatgcgc
      661 tggtggttgg ctggaatatt caggatacca cccgtctgtg gctggagggc tggattgcca
      721 gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta cgccctgaac
      781 tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc gcccaacagc
      841 agcaactcac cagttggcaa gaacaaggcc atgatatttt cccactcggc atttaatgat
      901 tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt gcaatatgcg
      961 catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttaggcg gaattggtgg
     1021 cgtcatgctc gcaggttata cctttatttt gcgtgctggc taagcgcctg caccagcctt
     1081 tcaaacaggc ggtctgcaat gatcgccgcc agtgccacca gtaacgcccc ctggatcaca
     1141 tacgcggtat taaatccgct aagcccgatg atgatgggcg tacccagcgt gctggcccct
     1201 accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc
     1261 agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc acgactcatt
     1321 cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg ctcccagccc ggcaagtgtc
     1381 gcctgcagga cgggcagcac accgtaaagg atcaaggcga taatcgctgg ttgcagacca
     1441 aagccgatca ccggaacggc aatcgccagc actgcgacag gcggaaaagt ctgtccaacg
     1501 gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt gacagcaatt
     1561 ccggcaccag tgccaatgat caccacaaac aaactcgaaa ttcccaccag ccagaaatga
     1621 gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag ttgtgggaac
     1681 aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagtgccac aaacaaagca
     1741 atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcct ccagcagatc
     1801 ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc accaccggca gcacctcgca
     1861 tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc
     1921 accttctgcc cgttcttcgc gacacacgta atccgccaca ctacgtaacg aaagcaggcg
     1981 cacacccagt tcgctgcgcc caaaaaactg gcggacaaaa tcgtttgccg gacgagtcag
     2041 catcgtcagc ggattgccct gctgcaccac ttcaccgtga tccatcaata ccagatgttc
     2101 tgccagccgt agcgcctcat caatatcatg agtgaccagc acaatggtac gccccagcaa
     2161 acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc
     2221 aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg ccactcccac
     2281 acgttgctgc tgaccaccgg aaagctgatg cggataacgc tcacgtaaat ttgactccag
     2341 ccccagtagc gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg accatttttg
     2401 taattgcggc acggtagcga tgttttgcgc cacgctccag tgggggaaca ggccaataga
     2461 ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc
     2521 tccggcaaag cggatcactc cgctgtcatg ctccaccagg cggttaatca ttttcagggt
     2581 ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt cctgaaaatt
     2641 gagattgaga tcgttaacgg ctttttgtgc gccgaacagt ttgctgacat ggctaaattc
     2701 aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagcgcg tcggtaagca
     2761 ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggcg ctgcttagca
     2821 gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca
     2881 tacctacagt ttgcaccatc accacccgca ggctgcgcag aaataccggt aacgccagtg
     2941 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcactctcca
     3001 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3061 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcttcc
     3121 ccagccacgg aaaggccgtc accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     3181 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     3241 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     3301 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     3361 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     3421 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     3481 catcgctaca ggccagcaat gccagcgccg cagccagcca gaaaccactg ccgaggctgg
     3541 tacgcgccag cgcactgcca ttttgcgcca gttgtgtcgc cgcctttccc gcgccccaca
     3601 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaca aatgctgcct tttttaccgg
     3661 gaataaaaca ggccgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     3721 gccacagctg ccagagatga cgcccctcac ccgaaactaa acgattaggc gcgtaactga
     3781 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     3841 tacggaaata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     3901 ccactttttt ggcatccagt ccttccacag caatgctggc attcagttgc tgcaatgttt
     3961 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactccttca
     4021 acaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacacct tgcggatcgc
     4081 ttaaggtttg cagccccagc gccgctaccg ggccgtcagt gccgtaagcc attgcagcat
     4141 taacgccaga gatttgctgg gcagcggctt tgatcgtcac cgccgtgtcg ccaccagcca
     4201 gtgacagcaa ctgatcctga ccgagcttaa agccataggc tttttcaaac gcgggtaacg
     4261 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     4321 gataacgact caggtcggca agcgaagtga gtttattttt ctctgccaca tcctgacgca
     4381 cggcgatagt ccaggtgtta tttgcaggcg cgggcgtcag ccagattaac ttgttgtgct
     4441 ccgaatcgag ctttttgact ttctcgtaac cttgctgcgc gtttttccac gctgcatcgt
     4501 tttcatcttt aaagaagaaa gcgccattgc cggtatattc cggatagata tccagttcac
     4561 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     4621 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     4681 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     4741 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     4801 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     4861 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     4921 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     4981 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5041 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5101 aggctcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     5161 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     5221 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     5281 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcc gtcactttgc cgtcacgctt
     5341 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     5401 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     5461 cttgtcggca ttatacggac gaccggtgtt cagatggctg tagtacaccg ggatctgccc
     5521 gacagaacgc gggaaggaca tcggcagctt gccggacggg ttgtaatcgc caaacaacac
     5581 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     5641 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     5701 tttaccggtg gctttcagcg ccgcaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     5761 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccat
     5821 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     5881 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     5941 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6001 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcgcg
     6061 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6121 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgtg cttctttacg
     6181 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     6241 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     6301 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     6361 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     6421 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     6481 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     6541 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     6601 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     6661 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     6721 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     6781 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     6841 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     6901 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     6961 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7021 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7081 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7141 gttatccggg ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     7201 cttaagcaat tcggtgacga acgcatcccg cgcttcgggc gtgaatggat ggttgccgaa
     7261 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     7321 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     7381 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     7441 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     7501 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     7561 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     7621 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     7681 agctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     7741 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     7801 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     7861 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     7921 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     7981 ggcggtattt gtaacaactc cggcggctca ctggtgcaac gtggcccggc gtataccgaa
     8041 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8101 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     8161 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     8221 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     8281 tcttcaggtt gcgccgggaa gctggcggtc tttgcagtac gtctagatac cttcgaagcg
     8341 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     8401 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatgcaccgg
     8461 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     8521 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     8581 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     8641 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     8701 ctgttaaaaa tggcgggcga tggcgtcggt gaagccaaat cgtggctggt agattatttc
     8761 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     8821 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     8881 gaagacattc tggcgttgga tatcgctctg cagcgtaacg acaccgagtg gtatgagcat
     8941 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9001 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9061 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9121 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
     9181 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
     9241 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
     9301 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
     9361 ggcatcactt taccggtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
     9421 tatttgccaa acgcatccat cactaccagc gccaccggtt tattattgat aaccgtacgc
     9481 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
     9541 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
     9601 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
     9661 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
     9721 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
     9781 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
     9841 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
     9901 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
     9961 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10021 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10081 atcgcgctac cggaggcaat ttcaggttgt gaagcggtga tagcggctgc cgttttcgca
    10141 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    10201 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    10261 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    10321 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    10381 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    10441 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    10501 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    10561 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaaa ccgcttaaaa
    10621 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    10681 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    10741 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    10801 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    10861 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    10921 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    10981 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11041 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11101 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    11161 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    11221 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    11281 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    11341 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    11401 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    11461 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    11521 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    11581 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    11641 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    11701 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    11761 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    11821 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    11881 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    11941 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12001 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12061 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgctttg
    12121 gttaacccac cgagcgcatg tttagcggct gtgtaggcgc tggcatccgg cagcggcgta
    12181 tgttcatgta ccgatgtaat gttgatgatg cgaccgccct gcccttgttt caccatctgg
    12241 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    12301 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    12361 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    12421 agtttaccga gatccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    12481 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccttgc
    12541 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    12601 acctgtgcca tcgagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    12661 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatggagg catccagcca
    12721 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    12781 gacgcgggaa ccagcaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    12841 caaggtatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    12901 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    12961 agacaagttg ctttcggctt ttgcgatatc gagattggcg ttaagacgac cactatcgaa
    13021 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13081 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13141 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    13201 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    13261 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    13321 ccgtgcctca ataattttca ttttccccgc gacatcgttg
//
LOCUS       DAJVTY010000114        13065 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVTY010000114 DAJVTY010000000
VERSION     DAJVTY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13065
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.114"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..257
                     /locus_tag="OQQ66_002899"
                     /pseudo
     CDS             1..257
                     /locus_tag="OQQ66_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            428..775
                     /locus_tag="OQQ66_002900"
     CDS             428..775
                     /locus_tag="OQQ66_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1905756.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            765..1127
                     /locus_tag="OQQ66_002901"
     CDS             765..1127
                     /locus_tag="OQQ66_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1905757.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1124..1621
                     /locus_tag="OQQ66_002902"
     CDS             1124..1621
                     /locus_tag="OQQ66_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1905758.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1629..2813)
                     /gene="emrD"
                     /locus_tag="OQQ66_002903"
     CDS             complement(1629..2813)
                     /gene="emrD"
                     /locus_tag="OQQ66_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1905759.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFLFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3232..3321)
                     /gene="tisB"
                     /locus_tag="OQQ66_002904"
     CDS             complement(3232..3321)
                     /gene="tisB"
                     /locus_tag="OQQ66_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1905760.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            3886..3984
                     /gene="ivbL"
                     /locus_tag="OQQ66_002905"
     CDS             3886..3984
                     /gene="ivbL"
                     /locus_tag="OQQ66_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1905761.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4090..5778
                     /gene="ilvB"
                     /locus_tag="OQQ66_002906"
     CDS             4090..5778
                     /gene="ilvB"
                     /locus_tag="OQQ66_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1905762.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5782..6072
                     /gene="ilvN"
                     /locus_tag="OQQ66_002907"
     CDS             5782..6072
                     /gene="ilvN"
                     /locus_tag="OQQ66_002907"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1905763.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6146..6736
                     /gene="uhpA"
                     /locus_tag="OQQ66_002908"
     CDS             6146..6736
                     /gene="uhpA"
                     /locus_tag="OQQ66_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1905764.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6736..8238
                     /gene="uhpB"
                     /locus_tag="OQQ66_002909"
     CDS             6736..8238
                     /gene="uhpB"
                     /locus_tag="OQQ66_002909"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1905765.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8248..9567
                     /gene="uhpC"
                     /locus_tag="OQQ66_002910"
     CDS             8248..9567
                     /gene="uhpC"
                     /locus_tag="OQQ66_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1905766.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9705..11096
                     /gene="uhpT"
                     /locus_tag="OQQ66_002911"
     CDS             9705..11096
                     /gene="uhpT"
                     /locus_tag="OQQ66_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1905767.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(11141..12907)
                     /gene="adeD"
                     /locus_tag="OQQ66_002912"
     CDS             complement(11141..12907)
                     /gene="adeD"
                     /locus_tag="OQQ66_002912"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1905768.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLDL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVYQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFVFTTLEVTE"
BASE COUNT         2909 a         3409 c         3646 g         3101 t
ORIGIN      
        1 caagcagctc tggagtggcg gtcaggtgct tgctgttccg ccagcgttgc gcgtgctgtg
       61 gtcatacagc gaaagaaaat cgcctgtcac aaagtaaatt cagatgccag gtatgtggat
      121 atacagcgaa cgccgatgta aatggcgctc gtaacatttt agcggcgggg cacgccgttc
      181 ttgcctgtgg agagatggtg cagtcaggcc gcccgttgaa gcaggaaccc accgaaatga
      241 ttcaggcgac agcctgaacg tagcagggat cctcgtcctt cagggcgtgg aggatgtcaa
      301 tttgcgctat gatacccatc tgacctcctt gtagcggatg gcaaaagcat atcaggaaac
      361 gggcggacga aattcttaaa aaaaaccgct aaagatagag cgttagtgaa attgcaaagg
      421 agcaacaatg aaaatttccc gccttggaga agcgcccgat taccgcttct cgctggctaa
      481 tgagcgtact tttctggcgt ggatccgtac cgcactagga tttctggcgg cgggggttgg
      541 gcttgatcaa cttgcgccag atttcgccac gcccgtcatt cgtgaactgc tggcgttgtt
      601 gttgtgtctg ttttccggcg ggttagcaat gtatggctac ctgcgctggt tacgcaatga
      661 aaaagcgatg cgtctgaaag aggacctgcc ctacaccaac agcttattaa tcattagctt
      721 aatattgatg gtcgtggcgg tgatcgttat gggactggtg ttgtatgccg gatagccgca
      781 aagccagacg cattgccgac ccaggactgc agccggagcg cacatcactg gcgtggtttc
      841 gtaccatgct gggctacggc gcgttgatgg cgttggctat caaacacaac tggcaccagg
      901 cgggcatgtt attctggatt tcgattggca ttctcgccat cgtggcgctg atcctctggc
      961 actacacccg taatcgcaat ttaatggatg tcacgaatag cgatttttcc caatttcacg
     1021 tagtccgtga caaatttttg atctccctcg cggtgttatc tctcgcaata ctgtttgctg
     1081 taacgcatat acatcaactt atcgtattta ttgagagagt cgcatgacag gaagtcaggt
     1141 cattgatgct gaagaggaca ggcataaact ggttgtagag tacaaggatg ctctgcaacc
     1201 tgccgatttt tatcacaatt tcaaacagcg gggcatccgc tccgtacagc tcattcccta
     1261 tcttgaattt gatgaccggg gcgatctgac ggccgcttcg gtgacagcag agctgtgggg
     1321 gaaattttta attgccctgt tcgagtgttg ggtgcgggca gatatcagtc gtatctcgat
     1381 tgagcttttt gacgccacct tacaaaaatg gtgtggcggc gaaaacccgc aaccgcgccg
     1441 tgactgccag gcgtgcgact ggcatcgcct gtgcccgcac gctcggcagg agacgccaga
     1501 cagcgtgctt tgtgcgggct atcaggcgtt ttattcttac tccgcaccgc atatgcgggt
     1561 gatgcgtgat ctgataaagc agcatcgttc tcccatggag ctgatgacga tgctgcggtg
     1621 acgtgcgctt aaacgggctg cccctgatgc gacatccgcg tcgccagcgg cagccagcac
     1681 agcacgatca acaatcccat taaggtcatc aataacccca ggctaccctg accggtttgc
     1741 ggcaacatcg cagagagcga cgccagcacg ccggaaccaa tgttttgcag accgccgacc
     1801 agcgcgccag ccgtgcccgc caggaagagg aacggctcca tcgcgccgct ggtcgccagc
     1861 ggaaacagca tcccggcacc gaaaaagaac agcgcggcgg gaacgagcag cgtccagaca
     1921 ttcatcacgc caaaccagtc ggggatccac atcagcaagc cagccagcag gcagcagata
     1981 accgactgcc acattaacgt ggagaagcgt ttattgggac gtccggcaaa ccatgcgcca
     2041 aaaaacgctg ccggaatcgg cagaataaac aaaatactga ccgtcatact gctcagccct
     2101 aataccgcgc ccatcagcac gccggagcag gcttcaaagg cggcaatccc ggccagaccg
     2161 ccaatcagca tcagcaaata acagttaaaa ccgctgttac cgaaaagcgt tttataactg
     2221 gtaagcaggc gcgtgcgcgg tgcatcgacc ggacgcgttt ccggcatcca gcgggccata
     2281 ctgaaggtca caccaacaca aagaaccaac aaaaagagat aacaggcgcg ccagttccac
     2341 atcgtatcca gcagaccgcc gattagcggt gcgagcaacg gactgacgag aatccccatg
     2401 tttaacaggc tgttagcatg gcgcaactgt gtccgttcat ataaatctcg cggcaaagta
     2461 cgcgccatta cgccgccaac gccggtgccc atcccctgca tcgcgctggc ggcaatcaat
     2521 accgtcaaac tggaggtcgt gaccgcgacc agcgttgcca gcataaaaat ggacattccg
     2581 acgaggatca ccggtcggcg acccacacgg tcggaaatcg ggccataaaa cagctgtgag
     2641 acaccgtaag tcagcagata agcgcccatt acgctctgca ccgccccttc acggacgttg
     2701 agatcgcgcg ccatatcggc aatagctgga atataaatgg tttgcgccat ctgaccgacg
     2761 gccacgagta ataccaacat caataacaaa ttgacgtttc tttgcctttt cattatcacg
     2821 gatgctttta taaaaataat tagaataagt cactacgcat tcccataaat gcgggagagg
     2881 aatctatcac attgatgaat gttagccaga tatacgccag gaatggggaa ttgtttagcg
     2941 gcagaatatg taaacaaaag cggcaataaa tgttgccggg agggattacg tgcgaactgt
     3001 aaccctctcc ccaaaggggc gagggggaaa ccaccgttcc ctttcagaaa atgagctaaa
     3061 cattgcacgg gacggtcccc tcgccccttt ggggagaggg ctagggtgag gggcacaggc
     3121 agcgcaggtg caaacattct gctggtagaa gaagccccca actccaaccc gcacgctaaa
     3181 tataaaaggg gagcggttac ccgctcccct ttggtgcgac ttgaatctga attacttcag
     3241 gtatttcaga acagcatcaa gcagttgcag tgctgcaaca atgagtttga ggataagaat
     3301 ggcgatatcc accaggctca tacgcgtctc ctgtggttca ggagcactgc cagctgacgt
     3361 acctttccgc tgcctgttgc cagctgttgt gcacgcggta tctcaaggag aaacgttgtg
     3421 cttgttgaca taacacagtg tgctcacgcg gggttaaggc gctgacggca ccacccgttt
     3481 cagccaggac ttagtgcgcc gggtaacgat gcggcccccg cataatacat tgcgtacagt
     3541 gatattatag aagtttactg tataaataaa cagtgatatt tggacaaaac gcaaactgtg
     3601 atcgatactc aactatgccc taaatacgtc aaaattcgtg ccgaaattgc gcgttctgcg
     3661 cggaacacgt atactttcag tgttgacata atacagtgtg ctttgcggtt accagctgca
     3721 ggcggctgat gaaacctcgc tccggcgggg ttttttgtta cctgcaattc agtacaaaac
     3781 gtgatcagcc cctcaatttt cccttgctga aaaattttcc attgtctccc ctgtaaagct
     3841 gtgcttgtat aaatattgtt aaacacaaaa ccaacaaggt ccccaatgac tacttccatg
     3901 ttcaacgcaa aactactacc aactgcgcta tccgccgcag tggtcgtcgt gcgtgtggtg
     3961 gtggtcgtcg gcaatgcgcc gtagggactg gaacaacaca cgattccaaa accccgccgg
     4021 cgcaaaccgg gcggggtttt tcgtttaagc acctcccgga aagtcggccc agaagaaaag
     4081 gactggagca tggcaagttc gggcacaaca tcgacgcgta agcgctttac cggcgcagaa
     4141 tttatcgttc atttcctgga acagcagggc attaagattg tgacgggcat tccgggcggt
     4201 tctatcctgc ctgtttacga tgccttaagc caaagcacgc aaatccgcca tattctggcc
     4261 cgtcatgaac agggcgcggg atttatcgct caggggatgg cgcgcaccga cggtaaaccg
     4321 gcggtctgta tggcctgtag cggaccgggt gcgactaacc tggtgaccgc cattgccgat
     4381 gcgcggctgg actccatccc gctgatttgc atcaccggtc aggttcccgc ctcgatgatc
     4441 ggcaccgacg ccttccagga agtggacacc tacggcatct ctatccccat caccaaacac
     4501 aactatctgg tcagacatat cgaagaactc ccgcaggtca tgagcgatgc cttccgcatt
     4561 gcgcaatcag gccgcccagg cccggtgtgg atagacattc ctaaggatgt gcaaacggca
     4621 gtttttgaga ttgaaacaca gcccgctatg gcagaaaaag ccgccgcccc cgcctttagt
     4681 gaagaaagca ttcgtgacgc agcggcgatg attaacgctg ccaaacgccc ggtgctttat
     4741 ctgggcggcg gtgtgatcaa tgcgcccgca cgggtgcgtg aactggcgga gaaagcgcaa
     4801 ctgcctacca ccatgacttt aatggcgctg ggcatgttgc caaaagcgca tccgttgtcg
     4861 ctgggtatgc tggggatgca cggcgtgcgc agcaccaact atattttgca ggaggcggat
     4921 ttgttgatag tgctcggtgc gcgttttgat gaccgggcga ttggcaaaac cgagcagttc
     4981 tgtccgaatg ccaaaattat tcatgtcgat atcgatcgcg cagagctggg taaaatcaag
     5041 cagccgcatg tggcgattca ggcggatgtt gatgacgtgc tggcgcaatt gatcccgcag
     5101 gtggaagcgc aaccgcgtgc agagtggcac cagttggtag cggatttgca gcgcgaattc
     5161 ccgtgtccaa tcccgaaagc gtgcgatcca ttaagccatt acggcctgat caacgccgtt
     5221 gccgcctgtg tcgatgacaa tgcgattatc accaccgatg tgggtcagca tcagatgtgg
     5281 accgcgcaag cttatccgct caatcgccca cgccagtggc tgacctccgg tgggctgggc
     5341 acgatgggtt ttggcctgcc tgcggcgatt ggcgcggcgc tggcgaaccc ggatcgcaaa
     5401 gtgttgtgtt tctccggcga cggcagcctg atgatgaata ttcaggagat ggcgaccgcc
     5461 agtgaaaatc agctggatgt caaaatcatt ctgatgaaca acgaagcgct ggggctggtg
     5521 catcagcaac agagtctgtt ctacgagcaa ggcgtttttg ccgccaccta tccgggcaaa
     5581 atcaacttta tgcagattgc cgccggattc ggcctcgaaa cctgtgattt gaataacgaa
     5641 gccgatccgc aggctgcatt gcaggaaatc atcaatcgcc ctggcccggc gctgatccat
     5701 gtgcgcattg atgccgaaga aaaagtttac ccgatggtgc cgccaggtgc ggcgaatact
     5761 gaaatggtgg gggaataagc catgcaaaac acaactcata acaacgtaat tctggagctc
     5821 accgttcgca accatccggg cgtaatgacc cacgtttgtg gcctttttgc ccgccgcgct
     5881 tttaacgttg aaggcattct ttgtctgccg attcaggaca gcgacaaaag ccatatctgg
     5941 ctactggtca atgacgacca gcgtctggag cagatgataa gccaaatcga taagctggaa
     6001 gatgttgtga aagtgcagcg taatcagtcc gatccgacga tgtttaacaa gatcgcggtg
     6061 ttttttcagt aaccgctcaa ggcttgaaca acatcgcgct tatcgttaag gtaagcgcgt
     6121 tttttttacc cgccaggaca agaccatgat caccgttgcc cttatagacg atcacctcat
     6181 cgtccgctcc ggctttgcgc agctgctggg gctggaacct gatttgcagg tagttgccga
     6241 gtttggttcg gggcgcgagg cgctggcggg gctgccgggg cgcggtgtgc aggtgtgtat
     6301 ttgcgatatc tccatgcccg atatctccgg tctggagctg ctaagccagc tgccgaaagg
     6361 tatggcgacg attatgctct ccgttcacga cagtcctgcg ctggttgagc aggcgcttaa
     6421 cgcgggggca cgcggctttc tttccaaacg ctgtagcccg gatgaactca ttgctgcggt
     6481 gcatacggtt gccacgggcg gctgttatct gacgccggat attgccatta aactggcatc
     6541 cggtcgtcag gacccgctaa ccaaacgtga acgccaggtg gcggaaaaac tggcgcaagg
     6601 aatggcggtg aaagagattg ccgccgaact gggcttgtca ccgaaaacgg tacacgtcca
     6661 tcgcgccaat ctgatggaaa aactgggcgt cagtaacgac gtagagctgg cgcgccgcat
     6721 gtttgatggc tggtgatgaa gacgttgttc tcccgcttaa ttaccgttat tgcctgcttt
     6781 tttatcttct ctgccgcatg gttttgcctg tggagtatca gcctgcatct ggttgagcgc
     6841 cctgatatgg cggtgctgtt atttccgttt ggtctgcgtc tggggctaat gctgcaatgc
     6901 ccgcgcggat actggccggt gctgctgggc gcggagtggc tgctgattta ctggctaacg
     6961 caggcggtcg gtttaaccca ttttccgtta ttgatgatcg gtagtttact gacgttactg
     7021 cccgtagcgc tgatctcgcg ctatcgccat cagcgtgact ggcgcacctt gctgttacag
     7081 ggggcggcgt taacggcggc ggcgttgttg cagtcgctgc cctggctttg gcacggcaaa
     7141 gagtcgtgga atgcgctgtt gctgacttta actggcggcc tgacgctggc cccgatatgt
     7201 ctggtgttct ggcactatct cgccaataac acctggctgc cgctcggccc gtcactggtt
     7261 tctcagccaa tcaactggcg cgggcgacat ctggtctggt acttgctgct gtttgttatc
     7321 agtctctggc tccagttggg attgccggac gaactgtcgc gctttacgcc attctgtctg
     7381 gcgctgccga ttatcgcgct ggcctggcac tatggttggc aaggggcgct gattgcgacg
     7441 ttgatgaacg ccatcgcgct gatcgccagt caaacctggc gcgatcatcc ggtggattta
     7501 ttgctctcgc tgctggtgca aagtctgaca gggttgttgc ttggcgctgg catccagcgg
     7561 ttgcgtgaac ttaaccagtc gctgcaaaag gaactggcgc gcaatcagca tctggctgaa
     7621 cggttgctgg aaaccgaaga gagcgtgcgc cgtgatgtgg cgcgtgagct gcatgatgat
     7681 atcggtcaga ccatcactgc tattcgtact caggcgggca ttgttcagcg gctggcggca
     7741 gataacgcca gcgtgaagca gagcgggcag ctcatcgaac aactatcgct gggcgtttac
     7801 gacgcggtgc gccgtttgtt gggtcggtta cgtccgcgcc agttggatga tctcaccctg
     7861 gagcaggcca tccgctcact gatgcgggaa atggagctgg aagggcgcgg tattgtcagc
     7921 catctcgaat ggcgaatcga tgaatcagcg ttaagcgaaa accagcgcgt gacgctgttt
     7981 cgtgtctgcc aggaagggct gaacaacatt gtgaaacatg ctgatgccag cgcggtcacc
     8041 ctgcaaggct ggcagcagga tgaacggttg atgctggtta ttgaagacga tggcagcggt
     8101 ttgccgccgg gttccgggca acaaggtttt ggcctcaccg gaatgcgcga gcgcgtaacg
     8161 gcgctgggtg gcacattaca catttcctgt ctgcacggca cgcgtgtcag cgtttctcta
     8221 cctcaacgct atgtctaagg tttgatgatg ttgccgtttc tgaaagcgcc tgccgatgcg
     8281 ccattaatga ctgataaata tgaaattgat gcccgctatc gctactggcg tcggcatatt
     8341 ctgctgacca tctggctggg ttacgcgctg ttttacttca cgcggaaaag ttttaacgcc
     8401 gccgtaccag aaatccttgc taacggcgtg ctcagccgta gcgatatcgg cctgttagcg
     8461 accctgtttt acattaccta tggcgtgtcg aagtttgtct ccggcattgt cagcgatcgc
     8521 tcaaatgccc gttattttat ggggataggg cttatcgcca cgggcattat caacattctg
     8581 tttggcttct cgacgtcgct atgggcgttt gccgtactct gggtgctgaa cgcctttttc
     8641 cagggctggg gttcaccggt gtgtgcgcgt ctgttaacgg cctggtattc acgtaccgag
     8701 cgcggtggtt ggtgggcatt atggaacacg gcgcataacg tcggcggcgc actcattccc
     8761 attgtgatgg cagcggctgc actacattac ggctggcgtg ccgggatgat gattgctggt
     8821 tgtatggcga tcgtcgtggg aatttttctc tgctggcggc tgcgcgatcg cccgcaggcg
     8881 ttaggtttac cggcggtcgg tgaatggcga cacgacgcgc tggagatagc tcaacaacaa
     8941 gaaggggcag ggctgacgcg taaagagatc ctcaccaaat atgtgttgct gaatccgtat
     9001 atctggctgc tttcgttttg ctatgtgctg gtctatgtgg tccgggcggc gatcaacgac
     9061 tggggcaatt tgtatatgtc cgagacgctg ggcgtcgatc tggtcacggc gaatacggca
     9121 gtgacgatgt ttgaactggg tggatttatc ggtgcgctgg tagcgggttg gggctcggac
     9181 aaattgttta acggcaaccg agggccgatg aatttgattt tcgccgccgg aattttgctt
     9241 tcagtcggct ccctgtggct gatgccattt gccagctacg tgatgcaggc aacctgcttc
     9301 ttcaccattg gtttttttgt ctttggccca cagatgttaa tcggtatggc ggcggcagag
     9361 tgttcccaca aagaggcggc aggggcggcg acggggtttg tcggcttgtt tgcttatctg
     9421 ggggcgtcgc ttgctggttg gccgctggcg aaagtactcg atacctggca ctggagcgga
     9481 ttttttgtgg ttatctctat cgccgccggg atttccgcac tgctgttact gccctttttg
     9541 aacgcccaga caccgcgcga agcgtgatgc atctcacctt ttcacttcat atccggcaaa
     9601 actaagaaat tttccaggtt ttgcctggac gctatctcag gcctgatttg ctgctgattt
     9661 ttacaatgca tgcctcacgc aggtattcat ttcaggagta acccatgctg gctttcctaa
     9721 accaggttcg caagccgacc ctggaccttc cgctcgaagt gcggcgcaaa atgtggttca
     9781 aaccgttcat gcaatcctac ctggtggtct ttatcggcta cctgacgatg tacctgattc
     9841 gcaagaactt taacatcgcg cagaacgata tgatttcgac ctacgggttg agcatgacgc
     9901 agctggggat gatcggcctg ggtttctcca tcacttatgg cgtgggtaaa acgttggttt
     9961 catactacgc cgacggcaaa aacaccaaac aattcctgcc gttcatgctg atcctgtctg
    10021 ccatttgtat gctgggcttc agtgccagta tgggcagcgg ctcggttagc ctgttcctga
    10081 tgattgcctt ctacgcctta agcggctttt tccagagtac cggcggttcg tgcagttact
    10141 ccaccatcac caaatggacg ccgcgtcgta aacgcgggac attcctcggt ttctggaata
    10201 tttctcacaa ccttggcggt gcaggcgcag caggtgtggc gctgttcggg gcaaattacc
    10261 tgttcgatgg ccatgtcatc ggcatgttta tcttcccgtc gattatcgcg ctgattgtcg
    10321 gttttatcgg cctgcgttac ggcagcgact ccccagaatc ttatggcctc ggcaaagctg
    10381 aagaactgtt cggcgaggag atcagcgaag aggacaaaga gacagaatct accgatatga
    10441 ccaagtggca gatctttgtt gagtatgtgc tgaaaaacaa agtgatctgg ctgctgtgct
    10501 tcgccaacat tttcctctat gtggtacgta ttggtatcga ccagtggtca accgtatacg
    10561 cgttccagga actgaaactc tctaaagcgg tggcgattca gggctttacg ctgtttgaag
    10621 ctggtgcgct ggtcggtacg ctgctgtggg gctggctctc tgacctggcg aacggtcgcc
    10681 gtggcctggt ggcctgcatc gcgctggcgc tgattatcgc cacgctcggt gtgtatcaac
    10741 atgccagcaa cgaatatatc tatctggctt ctctctttgc gttgggtttc ctggtctttg
    10801 gcccgcaatt gttgattggt gtggctgctg ttggctttgt acctaaaaaa gcgattggcg
    10861 ctgccgatgg tattaaaggc acctttgctt acctgattgg tgacagcttt gccaagttag
    10921 gtctgggaat gattgccgat gggacgccgg tattcggcct taccggctgg gcaggcacct
    10981 tcgccgcact tgatatcgcc gcgattggtt gtatctgcct gatggcgata gtggcggtga
    11041 tggaagaacg caaaatccgc cgcgagaaaa aaattcagca gttgacagtg gcataaacgt
    11101 aactggtgac ttttgcccgg catgacgccg ggctttttta ttattccgtg acttccagcg
    11161 tagtgaagac aaacttctcg ccatcaaata gcccctgact ggttagtttg agcgcgggga
    11221 tcactggcag agaaagaaac gccatctgaa taaacggctc atcgggtaac ggaccgcatt
    11281 cacgggcggc ggctttcaag gcgtcaattt gttccgccag cgactgcgcc gtgtcggtgc
    11341 tcatcaggcc ggcaatgggc aacggcagat gactttggac ctggccgtta cgtaccacgc
    11401 acagcccgcc gccatcctga atcacctgat agaccgccag cgccatctct tcggcactgc
    11461 gaccgatcac cacaatatta tggctgtcat ggctgaccgt cgcagccagc gcaccttcat
    11521 tcagtccaaa gccgccaagc aaaccacaag ccggagccag ccgttgcccg taacgctcaa
    11581 gtacggcaat aaagcacaca tcatcgcgat caaaaccatt ttcgctgtag acgctggagt
    11641 gggagtgcgt aatcaattcg ttatggatga cgtcaatgac ccgatagcgt tttccgggcg
    11701 taaattgcag ggcaaagtcg ctggcggaaa ctggctggcg ggcaatggtg ttgccatatg
    11761 gcggagcgga ttgtgccagt ctcgccgact cttccgcctg taaggtttgc gcatcaatcg
    11821 gctcgccttt caccagtacc tgctgcaccg tgaccttacg cgcatcgctc aacaggacga
    11881 tatcggcctg cttgccgggt gccagtaagt cgaggtgatt cagaccaaag tggcgcgccg
    11941 tcgaccagct ggcgacgcga tatgccacat gcagcggcac attgtgttgt tcgatcaggc
    12001 ggcgaattaa ggcatcgatg tgtccttcat gggcgatctc ccacgggtta cggtcatcgg
    12061 tacagagcat gcattgcggg ctgttaaatt cgttgatcaa cggtgccagc gcgttgagat
    12121 tgcgggcagc ggacccttcg cggatcatca acgacatgcc gagttgtaat ttccggcgtc
    12181 cttcttccag ctgataactt tcgtggcagt tttcaatacc cgcagtaata taggcgttaa
    12241 gttctttacc acccaaaccc gggcagtgac cgtccagcgt caggtggcga aatgcatcca
    12301 gtttatcgag cagcgcattc tgcccgctaa ttacgccagg gtagtccatc atttctgcaa
    12361 ggccggtaac ctgcggatgg tcccgccagg cgagcatctg ttcaagggta aaactggcac
    12421 cgttaacatc gcagccttcc agggcgggta cgcaagagct gacctgtaag tactggtttt
    12481 gccttgcctg ttcggcacag cgggcaaacc aggcgaatcc ggcttctccc atcacgttga
    12541 cgatttcatg agggtcgcaa ataacggtcg tcaggccgcg cggcagggta gcggtttcaa
    12601 aggtgaccgg cgtcatcatg ctggattcaa tatgcaggtg agcatcaata aaccctggca
    12661 ccgccgttgc gccgcgagca tcaatccgct gcaaagccgg agcatcagcg tattctgcgc
    12721 caacaccggc aatgtaacgt cctttaatca caattgggcc ggaaatttct ccgccattga
    12781 tcaggtcgag aatagagaca ttatcaataa tataatcggc aacagcgtcg ccacgggaaa
    12841 cggctaacaa ttcctggtat tcagcccggc taatgtgatg aaatttatgg ttaatggaat
    12901 tattcatatt tttctcattt tgattttatc aatttgatta ttatttatca taaagaaacg
    12961 tcgcgagatt attaaagaat atctattaat gtgcaattga aatgtgataa ttatcactga
    13021 accgttgttg tcatttgttt tacaaaagca agggattaaa tctcc
//
LOCUS       DAJVTY010000115        12980 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVTY010000115 DAJVTY010000000
VERSION     DAJVTY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.115"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            52..936
                     /locus_tag="OQQ66_002913"
     CDS             52..936
                     /locus_tag="OQQ66_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1905769.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OQQ66_002914"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OQQ66_002914"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS1905770.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OQQ66_002915"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OQQ66_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1905771.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OQQ66_002916"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OQQ66_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1905772.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OQQ66_002917"
     CDS             5160..6443
                     /locus_tag="OQQ66_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCS1905773.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8066
                     /locus_tag="OQQ66_002918"
                     /pseudo
     CDS             6590..8066
                     /locus_tag="OQQ66_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            8160..8246
                     /locus_tag="OQQ66_002919"
                     /pseudo
     CDS             8160..8246
                     /locus_tag="OQQ66_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8247..9068
                     /locus_tag="OQQ66_002920"
                     /pseudo
     CDS             8247..9068
                     /locus_tag="OQQ66_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            9092..9214
                     /locus_tag="OQQ66_002921"
                     /pseudo
     CDS             9092..9214
                     /locus_tag="OQQ66_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9259..9330
                     /locus_tag="OQQ66_002922"
                     /pseudo
     CDS             9259..9330
                     /locus_tag="OQQ66_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            9373..9876
                     /gene="yeaK"
                     /locus_tag="OQQ66_002923"
     CDS             9373..9876
                     /gene="yeaK"
                     /locus_tag="OQQ66_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS1905774.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(9877..9981)
                     /locus_tag="OQQ66_002924"
     CDS             complement(9877..9981)
                     /locus_tag="OQQ66_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905775.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            10150..10582
                     /locus_tag="OQQ66_002925"
                     /pseudo
     CDS             10150..10582
                     /locus_tag="OQQ66_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
     gene            complement(10539..11360)
                     /gene="nimR"
                     /locus_tag="OQQ66_002926"
     CDS             complement(10539..11360)
                     /gene="nimR"
                     /locus_tag="OQQ66_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS1905776.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            11457..12653
                     /gene="nimT"
                     /locus_tag="OQQ66_002927"
     CDS             11457..12653
                     /gene="nimT"
                     /locus_tag="OQQ66_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS1905777.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            12693..>12980
                     /locus_tag="OQQ66_002928"
     CDS             12693..>12980
                     /locus_tag="OQQ66_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCS1905778.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYA"
BASE COUNT         3300 a         3163 c         3079 g         3438 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tagcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aactgctgac tattctttgt
     7261 atgtcgtcga ctactgtccg gacaaccata ctgcgaccag gggaatcaac tatacaaaaa
     7321 tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
     7381 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
     7441 tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt
     7501 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggag ctgcaactct
     7561 ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca
     7621 acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttctgtgtc atgctggttg
     7681 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
     7741 tggtttcaat tgtcgatatt atccagcaaa gcatccgccc tgatgatatt ttagcgcgac
     7801 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
     7861 ttgcggatcg tatacgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg
     7921 ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac acgcgtaata
     7981 tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca
     8041 acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg
     8101 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
     8161 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
     8221 cactaactga aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta
     8281 tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg
     8341 acagagcaat gagtgcctat ctgcgctata tcgtgcagaa agcagactcc tcctttcttt
     8401 atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc
     8461 agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata
     8521 atacccatgg cttaaacctg actgctcata aatacccggg cttacgcggc acactacaaa
     8581 ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc
     8641 aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag
     8701 agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta
     8761 atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt attggtatcg
     8821 atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc atttaccagg
     8881 atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtgtatgtt gcagggcagc
     8941 taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc ttttacactc
     9001 atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc gattcggggc
     9061 gtgaagtggc caaattcagt gtgccacttc agaagccatc tcagtaatac gggaatacat
     9121 tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg gggaatatct ccccggcagc
     9181 cttcagggaa aaatatcatc agatggctgc ttaaaaaaag aacaaatggt agtgtccgct
     9241 attgccagta cacctcagtt acatgatgcg tataaagctt ccgatgagcg tttatatgtc
     9301 aataagcaga acaaaaacag ccgttcatga taaccttctg tggttgtttg cttgtaatct
     9361 caggagcgtg aaatgactga aatggctaaa ggaagcgtga cgcatcagcg gttaatcgct
     9421 ttattatcac aagaaggtgc taacttccgc gtcgttaccc atgaagcggt aggtaaatgt
     9481 gaagcggtgt cagaaattcg tggcaccgct ttaggccagg gagctaaggc actggtatgt
     9541 aaagtcaaag gcaatggcgt aaatcaacat gttctggcga tcctcgctgc cgatcaacag
     9601 gccgatctga gccaacttgc cagccatata ggcggattac gcgcttcgtt agccagccct
     9661 gctgaagtcg atgaactcac aggctgtgtc ttcggcgcga tccccccttt cagcttccat
     9721 ccaaaactca aactggttgc cgaccctcta ctcttcgaac gatttgatga aatcgccttc
     9781 aatgcaggga tgctggataa atccgttatt ttgaaaacca cggattattt gcgcattgcg
     9841 caaccagaac tggtcaattt ccgccgcact gcgtaactag cctgtccgtt caataagcag
     9901 aacggacaaa accagcacaa cagcaatggc aaaaaacgat gacgtgataa tcagtgtctc
     9961 gacgaacatt tgatcgttca tagcatgccc ccgggagata gctgttttca cgttattgtt
    10021 agcgtgcaca aatggcagtt tgatgacagt tcgcctgatt tttatgcaaa aaacgtaaag
    10081 attttctact tccttcctgc agcaagcgta aagtaagcag gcttattatt ttttggcaag
    10141 gaaaccacga tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt
    10201 atcagtcata acaccactgt cgccgtttca attctggtgt ttcacaccgt taagcacctt
    10261 tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga cgattggcgt
    10321 tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact cgtttctgaa
    10381 ctggaaatcg ctggtggcaa ttgcagtagg cgtgattgtc tcctggctgg gtgggcgcgg
    10441 cgtgacctta atgggcagcc agccgcaact ggtcgccggg ctgctggtag gcactgtttt
    10501 aggcgtggcg ctgtttcgcg gcgtaccggt cggcccgctt attgccgcgg gtctggtttc
    10561 gctgattgtg gggaaacagt agttaatccc gcaatatatc ggcctggcgt ttgccccagg
    10621 cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt atgagcaact
    10681 ttctgtacag tatcgccttt cactaatccc tgaagtgcca taatcagctg taactgctga
    10741 cgccactggc gaaaactcaa ccccgtctct tttacgatca gtcgcgcaag attgcgttca
    10801 ctcatcgcaa aaaagccagc ccattgccct aatgaccccc actcgacggg ccctttcgcc
    10861 atcatctcca ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg cagctgttgt
    10921 tgtggctgct gagggagttc atcaaacagg acctgtatta atctgcgggt catcggctca
    10981 gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca attcccggca taagggcgat
    11041 attttcaatg tgcaacatgt tgtcggcatt gtcactgcgc tgggttcgat aaacagaaaa
    11101 cagagttcag cgttagctgt cacttgattg ctatgttcca ccccgcccgg tatccaaacc
    11161 gcatattgcg gcggcaccat ccacaaagca ttttccaccg tacaggtaat tgcaccatgt
    11221 aatgctagta tcagctgtcc tttgcggtgc tgatgtacag gactggttaa ctcatccgtt
    11281 ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc atagccattg
    11341 agattcaacc tgtgcatcat tttgtccgaa tttagcgata atttgtcatt ttagcttgat
    11401 tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag aagcatatga
    11461 cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc attctgatga
    11521 ttgccacaac attacgcgtc acctttaccg gcgcagcacc gttactggat acgattcgtt
    11581 ccgcttactc gctgacgaca gcgcaaaccg gcttattgac caccctgcca ttattggcct
    11641 ttgcgctaat ctcacctttg gccgccccgg tagcgcgacg ttttggtatg gaacgtagcc
    11701 tgtttgccgc gttacttttg atctgtgctg gtatcgcaat tcgctctctc ccttcgcctt
    11761 acttattatt tggcggtaca gcggtcattg gcggtgggat tgcattaggc aatgtcttac
    11821 tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc ggcgcatatt
    11881 ccctgacaat gggagctgcg gcggcacttg gatcggctat ggtagtgccg ctggctttga
    11941 acggttttgg ctggcaaggc gcgttgctca tgctgatgtg ttttcctctg ctggctcttt
    12001 ttttatggct gccacagtgg cgaagccaac aacatgcaaa tttgagtacc tcgcgcgcct
    12061 tacatactcg gggtatctgg cgttcgccgc ttgcctggca ggtcacattg tttcttggga
    12121 gcaactcact ggtctattac gtgattattg gctggcttcc ggcgatcctc atcagtcacg
    12181 gctatagcga agcacaggcg ggttcactgc atggtttgct gcaactagcc acagcagctc
    12241 ccggtttgct gatccctctt ttcttacatc atgtgaaaga ccagcgtggt attgcagcgt
    12301 tcgttgcctt gatgtgcgca gtgggcgctg ttgggctctg ctttatgcca gcgcacgcga
    12361 tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg gggttgacgt
    12421 tcattggtct tcgggcaagt tctgcgcatc aggcggcggc actctcgggg atggcacaat
    12481 ccgtcgggta tttgctggca gcctgtgggc cgccgctaat gggtaaaata cacgatgcta
    12541 acggtaactg gtccgtacca ctgatgggtg ttgcaatact ttcactactg atggcgattt
    12601 tcggactttg cgccgggaga gacaaagaaa ttcgctcata tccggtgcta tagtgacgtg
    12661 acaaatcatg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa cgcgtttatg
    12721 atccggctga acagagcgat ggttatcgcg tactggtcga ccgcctctgg ccgcgcggta
    12781 tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg tcaacggaac
    12841 tgcgcaaagc ctttcacggc gaagtcgtcg attttacaac ctttcgcgag caatatcttg
    12901 cagaactggc gcaacacgag caagaaggaa agcggctggc ggacatcgcc aaaaaacagc
    12961 cgctgaccct gctctacgca
//
LOCUS       DAJVTY010000116        12943 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVTY010000116 DAJVTY010000000
VERSION     DAJVTY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.116"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..943)
                     /gene="ychF"
                     /locus_tag="OQQ66_002929"
     CDS             complement(<1..943)
                     /gene="ychF"
                     /locus_tag="OQQ66_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1905779.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEK"
     gene            complement(1060..1644)
                     /gene="pth"
                     /locus_tag="OQQ66_002930"
     CDS             complement(1060..1644)
                     /gene="pth"
                     /locus_tag="OQQ66_002930"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1905780.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1922..2200
                     /gene="ychH"
                     /locus_tag="OQQ66_002931"
     CDS             1922..2200
                     /gene="ychH"
                     /locus_tag="OQQ66_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1905781.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2255..3934)
                     /gene="dauA"
                     /locus_tag="OQQ66_002932"
     CDS             complement(2255..3934)
                     /gene="dauA"
                     /locus_tag="OQQ66_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1905782.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4059..5006)
                     /gene="prs"
                     /locus_tag="OQQ66_002933"
     CDS             complement(4059..5006)
                     /gene="prs"
                     /locus_tag="OQQ66_002933"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1905783.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5157..6008)
                     /gene="ispE"
                     /locus_tag="OQQ66_002934"
     CDS             complement(5157..6008)
                     /gene="ispE"
                     /locus_tag="OQQ66_002934"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1905784.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6008..6631)
                     /gene="lolB"
                     /locus_tag="OQQ66_002935"
     CDS             complement(6008..6631)
                     /gene="lolB"
                     /locus_tag="OQQ66_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1905785.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6845..8101
                     /gene="hemA"
                     /locus_tag="OQQ66_002936"
     CDS             6845..8101
                     /gene="hemA"
                     /locus_tag="OQQ66_002936"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1905786.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8143..9225
                     /gene="prfA"
                     /locus_tag="OQQ66_002937"
     CDS             8143..9225
                     /gene="prfA"
                     /locus_tag="OQQ66_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1905787.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9225..10058
                     /gene="prmC"
                     /locus_tag="OQQ66_002938"
     CDS             9225..10058
                     /gene="prmC"
                     /locus_tag="OQQ66_002938"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1905788.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGGREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10055..10447
                     /locus_tag="OQQ66_002939"
     CDS             10055..10447
                     /locus_tag="OQQ66_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1905789.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10451..11260
                     /locus_tag="OQQ66_002940"
     CDS             10451..11260
                     /locus_tag="OQQ66_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1905790.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDPVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11296..12150
                     /gene="kdsA"
                     /locus_tag="OQQ66_002941"
     CDS             11296..12150
                     /gene="kdsA"
                     /locus_tag="OQQ66_002941"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1905791.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12299..12406)
                     /locus_tag="OQQ66_002942"
     CDS             complement(12299..12406)
                     /locus_tag="OQQ66_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1905792.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(12834..12941)
                     /locus_tag="OQQ66_002943"
     CDS             complement(12834..12941)
                     /locus_tag="OQQ66_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1905793.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWHNRK"
BASE COUNT         3197 a         3313 c         3368 g         3065 t
ORIGIN      
        1 ctttttcaaa atcagtatgg attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa
       61 tggtccatgc acgcacttct ttcaccccag cggtgaagta agtttgcagg ttcagcagct
      121 tataaccggc acggatcaca cggttcaggc ccggctcttc cagcccaagc tcctgcataa
      181 actcgtcacg ttcttcgtcg tccagttcgg caatgtctgc ttcaacagca gcacaaaccg
      241 gaaccacaac agaaccttct ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt
      301 tttcaaaacc gtcttcgttg acgttggcga tgtacattgt tggtttcagc gtcaggaagc
      361 tcaggtaacg aatagccgct ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg
      421 cattttccaa ctggggcagg catttttcca ggaccgccag ctcagctttc gcgtctttat
      481 cgccaccttt ggctttcttc tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg
      541 ccagcgccag ttcggtgttg ataacttcaa tatcgtcagc cgggttaact ttgccggaaa
      601 cgtgaatgat gttgtcattt tcaaagcagc gaacaacgtg accgatcgct tcggtttcac
      661 ggatgttggt caggaactgg ttacccagac cttcgccttt cgatgcgcct tttaccagac
      721 cggcgatatc gacaaattcc atggtcgtgg gaagcgtacg ctgcggtttt acgatttcag
      781 ccagttgatc caggcgagga tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc
      841 agaatggaaa gttggccgct tcaataccgg ctttggtcag cgcgttgaac agggtagatt
      901 tcccgacgtt gggcaaaccg acgataccgc atttgaatcc catgatttaa atcaccttaa
      961 tatcttaata atcaacctgt tattgctaac agattgcaga aatggaaata actttgtcta
     1021 ttatacacgg cactcggcaa aaatgccgca gacaacgact tattgcgctt taaaggcgtg
     1081 caatcggttc gttgctttgg tcaagccatc tgtaaaccac atttcagtac aacgcgccgc
     1141 ttcgtcaatg gcttcatcaa ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa
     1201 accgacaact ttatttttat cgcccggatg accgattccg atgcgtaaac ggtgaaagtt
     1261 agggttatta cccaatttac tgatgatgtc tttcagtcca ttgtgaccac catggccacc
     1321 gcccaattta aatttggcga cgccaggagg cagatccagt tcgtcgtggg ccaccagaat
     1381 ttcgtccgga ttaatgcgga aaaaactggc catcgccgca acggctttgc cgctgagatt
     1441 cataaatgta gtcgggacta acaggcggac atcttcgcct ccaagagtga ctcgcgaagt
     1501 ataaccaaag aatttagcct cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc
     1561 aacgaaccag gcaccagcat tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag
     1621 gccgacaatc aatttaatcg tcacgttttt ttgtcctgag tgtgtacata actggcgcgt
     1681 agtttactgg ttgcgacccc gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat
     1741 aattgcctga gtgggctatt agaaagtcaa ggtgttcagg cgtttatttg taaagttttg
     1801 ttgaaataag ggttgtaatt gtgatcacgc ccgcacataa cccacagggt gttgtctata
     1861 ctttacacat aaggaagagg ggtattccct gttacaaccc agaaagttcc ggaggtgaca
     1921 tatgaaacgc aaaaacgctt cgttactcgg taacgtgctc atggggttgg gtctggtggt
     1981 aatggtggtc ggcgtggggt attcaatcct caaccagtta ccacagttta atatgcccca
     2041 gtatttcgca catggtgcag tgctaagtat tttcgtcggt gccattctct ggctggcggg
     2101 tgcccgtgtt ggcgggcatg aacaggtgtg cgaccgttac tggtgggttc gccactatga
     2161 caaacgttgc cgccgtagcg ataatcgccg tcatagctaa caaataatgc agtttgctga
     2221 ccagtcagtt tgcgctgact ggtcaatctc gtacttataa atccgccatc gccgcgcgac
     2281 gattcgggaa gaacgcgagg cgtcccgaga ttggttgaat gcctgcgcga gccatagtgc
     2341 gcagtggctg gaattccacg ttgcacacgc gcagttcaca tccttcgggc agacgcttca
     2401 caaaacgctg gaacgcatca agaccaccag catcaagtac cggaacggca tcccacttca
     2461 gaatcacaat ccgtttgcct tcaagacgtg actccaggtc cgtgaacaag ccttcagcag
     2521 cagcaaaaaa cagcgggcca ataacgcgca gtaccagaac atcgtctgga acatctacga
     2581 ctaccggtgc caggcgagtc atacgtgcga tacgacgcat aaacagcagc gatgccagca
     2641 cgatccccac gctgatggca ataaccatat caaacagcac ggtcagcgac atgcacagca
     2701 gcatgacaat gatgtcatct ttcggtgcat gacgcagcaa gtcgaccact ttatgcgctt
     2761 cactcatgtt ccacgccacc atcaacagca gggctgccat agcggaaagc ggcagccagg
     2821 agagcagcgg tgccagtacc agcagggcaa gaataaccag aatagagtgg atcaccgctg
     2881 agatagggga agttgccccg gcacggacgt tagcggcaga acgcgcgatg gcagctgtag
     2941 cggtaatacc accaaagaat ggagcgataa tattccccag tccctgtcca accagttcgc
     3001 tgttcgcctt gtgtttcgtc ccggtcatac catccagtac cacggcgcag agcagagatt
     3061 cgattgcgcc gagcattgcc attgagaatg ccgcaggcag cagtgtgcga atagaatccc
     3121 aggttagcgt gaattctgaa ttaggcagat cccacggcag caccagttgc ggcagcagtt
     3181 gcggaatacc gttaccctga gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg
     3241 tagcaacatg tccgccgagc aggttaacaa tccccatcac cgcgcaacca gccagcaagg
     3301 ccggaaggtg accgggtaaa cgaatgccca gacgcggcca aaaaacaaga atacctagcg
     3361 tcacaatgcc aatggcagca tcacccacat taatggtcgg cagcgccata aataatgcgc
     3421 cgactttttg tagataatgt tccgggacat gggccatttg cagaccgaga aaatctttaa
     3481 tctgcatggt accgatggtg atcccgatac ccgaggtgaa acctaaggtg acggaaaccg
     3541 gaatatactc aatcaggcga ccaaagcgtg ccagacccat cagaatcaaa aagatccccg
     3601 acagcaaggc cgcaaccagc agtcctgcca gtccaaactg ttgcgacacg ggatagataa
     3661 ttaccacaaa tgccgcagtc ggaccggaaa cgctaaagcg tgacccaccc gtcagagcaa
     3721 tgacaatccc cgcaacagct gcggtatata aaccgtactg gggtgccaca ccactaccaa
     3781 tagccaacgc catcgccagc gggatagcaa taatcccgac ggttatcccg gcaatcaggt
     3841 cacgggtaaa ccgtgcggca gtatattttt ctttccagca agcgtcgatc agagcgcgga
     3901 aaggcatcac atgtgaggaa aatattttgt tcacaataat gtttcatccg tgagcgcatc
     3961 atctgtcaac taaatggcag gtgaaggagg cataggtcat acaaatggat attacagaca
     4021 aaaaaacccg ccgcagcggg tctttgagcc gggttcgatt agtgttcgaa catggcagag
     4081 atcgattctt cgttgctgat acgacgaatc gcttcggcca gcatacctga cagggtcaga
     4141 gtacgcacgt tcggcagtga tttgatttca tcgctcagcg gaatggtatc gcagacaacg
     4201 acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga
     4261 gtcgcgtacg caaatacacg tttagcacca cgttctttca gagcttcagc agctttacac
     4321 agcgtaccgc cagtgtcgat catatcatcg accagtacgc agtcacgacc tgcaacgtca
     4381 ccgatgatat gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc
     4441 atatcggtat cgttcagcag cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc
     4501 ggagaaacca caattgggtt atccagattc agctgcagca tgtcttccag caggatcggg
     4561 ctaccaaata cgttatcaac cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga
     4621 tccactgtca gcacacggtc aacaccgacg ctggagagga agtctgcaac cactttcgca
     4681 gtgattggta cacgagcgga acggacgcga cggtcctggc gcgcatagcc aaagtagggg
     4741 ataacagcgg tgatacgacc tgcggaagca cgacgcaggg catcaaccat aacgactaat
     4801 tccatcaggt tgtcgttagt aggggcacaa gtggactgga tgatgaaaat atcaccaccg
     4861 cgtacatttt cattaatttg tacgctgact tcgccatcgc taaagcgacc tacagcggcg
     4921 tcgccgagtg aagtgtacag gcggttggca atacgttgtg ctagttccgg ggtggcgtta
     4981 ccagcaaaaa gcttcatatc aggcacgaga agaacctcag gcatgcgtcc attggtggaa
     5041 agaatctgcc gaaaactgtg cgggccaggc atgatccttt ccaggcagtg tattaaagag
     5101 cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa
     5161 agcatggctc tgtgcaatgg ggaaagatta acgcctttcg ccacaaagcc attgagccat
     5221 tccggggctt gctctagcac ctggcgggct tcagactctg tatcaaattc agcaaagaca
     5281 caggcccctg tcccagtcag gcgcgacggg gcgtattcta acagccagga aagcaccgca
     5341 tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat cattgctgaa ttcacatttt
     5401 agcaacgttt ctattgacct ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc
     5461 accggagtcg gaatacttac accagggtgc gccaccagat accacttctc tggcggatcc
     5521 accggcgtta gtatttcacc aacgccttcg gcaaacgcgg catgcccccg aacaaagaca
     5581 ggaacatctg cgcccagcgt cagccccatt tccgccagct catccatgct tagcccgcat
     5641 tgccagagat gatttaatgc caccaggacc gtcgcggcat tggatgaacc accgccgaga
     5701 ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga
     5761 cgcccgctgt ctgccgcagt tttcatcaac aatcgcgctg cgcgaacgat caggttatct
     5821 tcatgttcca cgccttcaac gggcgttaac agacgaatat ccccatcgtc acgaagctca
     5881 atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca
     5941 tccgcacgct gaccggtaat gtataaaaac agattaagtt ttgccggaga gggccactgt
     6001 gtccgcatta tttcactatc cagttatcca tttttaactt gatgcgttga ccaccgtcgg
     6061 tgagttccat attggctggc atcgcaggat gcgttttggt gtcataacca ccataaacaa
     6121 ccttccagtt tttgccattc tggctgtagg taatttcgct caggcggtac tggtcgtcca
     6181 gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg
     6241 gaattggcat tccggtcaat ttgccaatca tctcttcggc gtcatcggcg gtataacgct
     6301 gacctttatt gtcgactaac tgcacgttac ccggttgagc attcagctcc agttccgtgc
     6361 tgcccaatgg gttagtgagc agcagacggt agcgatcctg gccggtttgc tgccagaaaa
     6421 agcgggcgta cactttttgt tggtcagaaa tataagcgaa cgcgccgcga gtctgatact
     6481 gattaagatt gcgcacgtct tgctgatgct gacgccattg tggcgaatcc gggcttttgc
     6541 caggaccttt gggcgtggta acggaacagg cagtgagcac aagagcagcc agcggtagca
     6601 ggcggataag acgaaaatcg ggcaggggca tagtgatgac aagtccttga gatacgttgc
     6661 agttataacc cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa
     6721 ttgccagaat ctaacggctt tcggcaatta ctccaaaagg gggcgctctc ttttattgat
     6781 cttacgcatc ctgtatgatg caagcagact aactctatca acgttggtat tatttcccgc
     6841 agacatgacc cttttagcac tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga
     6901 acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc
     6961 gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt
     7021 tgaagagcag gataacctgc aagaggcgtt aatccgctgg ctttgcgatt atcacaatct
     7081 taatgaagaa gatctgcgta aaagcctcta ctggcatcag gataacgacg cggttagcca
     7141 tttaatgcgt gttgccagcg gcctggattc actggttctt ggggagccgc agatcctcgg
     7201 tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat atgaaggcca gcgaactgga
     7261 acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg
     7321 tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc
     7381 gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg
     7441 tcatctgcgc gaacataaag tacagaagat gattatcgcc aaccgcactc gcgaacgtgc
     7501 ccaaattctg gcagatgaag ttggcgcgga agtgattgcc ctgagtgaga tcgacgaacg
     7561 tctgcgcgaa gccgatatca tcatcagttc caccgccagc ccgttaccga ttatcgggaa
     7621 aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa ccaatgctgt tggtggatat
     7681 tgccgttccg cgcgatgttg agccggaagt tggcaaactg gcgaatgctt atctttatag
     7741 tgtggacgat ctgcaaagca tcatttcgca caacctggcg cagcgtaaag ccgcagcggt
     7801 tgaggcggaa actattgtcg ctcaggaaac cagcgaattt atggcgtggc tgcgagcaca
     7861 aagcgccagc gaaaccattc gcgagtatcg cagccaggca gagcacgttc gcgatgagtt
     7921 aaccgccaaa gcgttagcgg cccttgagca gggcggcgac gcgcaagcca ttatgcagga
     7981 tctggcatgg aaactgacta accgcttgat ccatgcgcca acgaaatcac ttcaacaggc
     8041 cgcccgtgac ggggataacg aacgcctgaa tattctgcgc gacagcctcg ggctggagta
     8101 gcagtacatc attttctttt tttacagggt gcatttacgc ctatgaagcc ttctatcgtt
     8161 gccaaactgg aagccctgca tgaacgccat gaagaagttc aggcgttgct gggtgacgcg
     8221 caaactatcg ccgaccagga acgttttcgc gcattatcac gcgaatatgc gcagttaagt
     8281 gatgtttcgc gctgttttac cgactggcaa caggttcagg aagatatcga aaccgcacag
     8341 atgatgctcg atgatcctga aatgcgtgag atggcgcagg atgaactgcg caaagctaaa
     8401 gaaaaaagcg agcaactgga acagcaatta caggttctgt tactgccaaa agatcctgat
     8461 gacgaacgta acgccttcct cgaagtccga gccggaaccg gcggcgacga agcggcgctg
     8521 ttcgcaggcg atctgttccg tatgtacagc cgttacgccg aagcccgccg ctggcgggta
     8581 gaaatcatga gcgccagcga gggtgaacat ggtggttata aagagatcat cgccaaaatt
     8641 agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatctg gcggtcatcg cgtgcagcgt
     8701 gttcctgcta cggaatcgca gggtcgtatt catacttctg cttgtaccgt tgcggtaatg
     8761 ccagaactgc ctgacgcaga actgccggac atcaacccag cagatttacg cattgatact
     8821 ttccgctcgt caggggcggg tggtcagcac gttaacacca ccgattcggc aattcgtatt
     8881 actcacttgc cgaccgggat tgttgttgaa tgtcaggatg aacgttcaca acataaaaac
     8941 aaagcgaagg cgctttcagt gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc
     9001 caacaggccg aagcgtctac ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt
     9061 aaccgtactt acaatttccc gcaggggcgc gttaccgatc accgcatcaa cctgacgctc
     9121 taccgcctgg atgaagtgat ggaaggtaag ctggatatgc tgattgaacc gattatccag
     9181 gaacatcagg ccgaccaact ggcggcgttg tccgagcagg aataatggaa tatcaacact
     9241 ggttacgtga agcaataagc caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa
     9301 tcctgctgga gcatgttacc ggcagagggc gtacttttat tctcgccttt ggtgaaacgc
     9361 agctgactga cgaacaatgt cagcaacttg atgcgctact gacacgtcgt cgcgatggtg
     9421 aacccattgc tcatttaacc ggggggcgag aattctggtc gttgccgtta tttgtttcgc
     9481 cagcgacctt aattccgcgc ccggatacgg agtgtctggt ggagcaggca ctggcgcggt
     9541 tgcctgaaca accttgccgt attcttgatc tcgggacggg taccggggcg attgcgcttg
     9601 cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg
     9661 tctccctggc acaacgtaat gcccagcatc tggcgatcaa aaatatccac attctgcaaa
     9721 gcgactggtt tagcgcgcta gccgggcagc agtttgcgat gattgtcagc aatccgccgt
     9781 atattgacga gcaggaccca catcttcaac aaggcgatgt ccgctttgag ccgctcactg
     9841 cgctggttgc ggcagacagt ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta
     9901 acgcgctggt atccggcggc tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg
     9961 tgcgacaagc atttatcctc gcggggtatc atgacgtcga aacctgccgt gactatggtg
    10021 ataacgagcg cgtaacgctc ggccgctatt atcaatgaca agtttttcta cactgcttag
    10081 tgttcatctt attagtatcg cgctttctgt tgggctatta accttacgtt tctggctacg
    10141 ttatcagaag catccacagg catttgctcg ctggacgcgc attgtgccgc cggttgtcga
    10201 tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc tgccattttc
    10261 cgggcaggca cagtggctga ctgaaaagct gtttggagtt atcatttata tcgttttggg
    10321 ttttattgca ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc
    10381 gctggcgttg gtggtgctgt acatcatcat taaactcgcc accacaaaag taccgttact
    10441 ggggtaagtc atgagatcgt tagctgattt cgaatttaat aaagcgccat tgtgcgaagg
    10501 catgatcctg gcttgcgaag caatccgccg cgattttccc tcgcaagatg tttacgacga
    10561 actggagcgt ctcgttagtc tggcgaagga agaaatcagc cagcttctgc ctttagaaga
    10621 gcagctggaa aaactgatcg cgctgtttta cggcgactgg ggatttaaag cctcacgcgg
    10681 cgtttatcgt ctttccgatg cattatggct ggacccggtg ttaaagaatc gacagggcag
    10741 tgcggtatca ttaggtgcgg ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct
    10801 gccggtgatt ttccctacgc agctgatatt gcgcattgaa tgtccggatg gcgaaatttg
    10861 gctgattaat ccttttaacg gtgaatcgtt aagcgaacat atgctggacg tatggttaaa
    10921 gggaaatatc agcccgtcgg cggaactgtt ttatgaagac cttgatgaag ctgataacat
    10981 tgaggtaatc cgcaaattgc tggatacact caaagcctcg ttgatggaag aaaatcagat
    11041 ggagctggcg ttacgcacca gcgaagcttt attacaattc aaccctgaag atccctatga
    11101 aattcgcgat cgcgggttga tttatgcgca actggattgc gaacacgttg cgttgaacga
    11161 tttaagttat ttcgttgaac agtgtccgga agacccgatc agcgaaatga tccgtgcgca
    11221 aataaataac atcgcgcata aacatattgt gctgcattaa ttaatcgaca ttttactcaa
    11281 gattaaggcg atcctatgaa acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat
    11341 gacctgccgt tcgtactgtt tggcggtatg aacgtgctgg aatctcgcga tctggcgatg
    11401 cgcatttgcg agcactacgt aactgtgacc cagaaactgg gtatccctta cgtgttcaaa
    11461 gcctcttttg acaaagccaa ccgctcctcc atccactctt atcgtggacc gggcctggaa
    11521 gaagggatga aaatcttcca agagttgaag cagacttttg gcgtgaaaat tatcaccgac
    11581 gttcacgaac caagtcaggc acagcccgtt gctgatgtcg tggatgtgat tcagttgccg
    11641 gcgtttcttg ctcgccagac tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt
    11701 aacgtcaaga aaccacagtt tgtcagcccg ggacagatgg gtaatatcgt tgataaattc
    11761 aaagaaggcg gcaacgaaaa agtgattctt tgcgatcgtg gtgctaactt cggctatgac
    11821 aacctggttg tcgatatgct gggcttcagc attatgaaga aagtgtctgg taactcgccg
    11881 gtgattttcg acgtgaccca cgcactgcaa tgccgcgatc cgtttggcgc agcttccggt
    11941 ggtcgtcgtg ctcaggtggc tgagctggca cgagccggta tggcggtagg tctggcgggg
    12001 ctgtttattg aagcgcatcc ggatccggaa catgcgaaat gtgatggtcc atccgcgctg
    12061 ccgctggcta aactggaacc gttcctcaag cagatgaaag cgattgatga tctggtgaaa
    12121 ggtttcgaag aactggatac cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc
    12181 gttctgcgtt aagacttttt tcatgggtgc cagatacaaa aaaggccgca ggctgttacc
    12241 cctgcggccg gtttcgggcg catattgcca tcacggcagc ctgacgcccg ttttcacctt
    12301 acttccggtt acgccaccag ccgacaatcg ctgcggtaat aattcccgcc aggatcggtg
    12361 ctgccaggtc gtgccagaaa atcatggcaa actgcgcgag cgtcatatag ccgccttgtt
    12421 gtaatgataa cattttgcgg ctattcttga attgttctgg ttcaagatta gcccccgttc
    12481 tattgtcagg ttaaacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac
    12541 cagggataaa gcccccgcaa cattgcgcct caccggataa cgccggcttg atgtggatac
    12601 tacgtctcaa ttcatcttca cttcatccct gaaatgtttg caatgaagag tgcattccgg
    12661 tttttcaaca gctgttacag tcgtttcatg agtgctctgg atgatgcttc cagctcgggt
    12721 tgccaatatt tacttgtgga agagataaag acaaaaatgg ccgcaggctg ttacccctgc
    12781 ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc accttacttc
    12841 cggttatgcc accagccgac aatcgctgcg gtaataatcc ccgccaggat cggtgctgcc
    12901 aggtcgtgcc agaaaatcat ggcaaacttc gcgagcgtca tat
//
LOCUS       DAJVTY010000117        12942 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVTY010000117 DAJVTY010000000
VERSION     DAJVTY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.117"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQQ66_002944"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQQ66_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1905794.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OQQ66_002945"
     CDS             1946..2230
                     /locus_tag="OQQ66_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1905795.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OQQ66_002946"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OQQ66_002946"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1905796.1"
                     /translation="MGQVLPLVTRQGDRIAIVSCLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OQQ66_002947"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OQQ66_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1905797.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVSERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OQQ66_002948"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OQQ66_002948"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1905798.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7227..7487
                     /locus_tag="OQQ66_002949"
     CDS             7227..7487
                     /locus_tag="OQQ66_002949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1905799.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7494..7790
                     /locus_tag="OQQ66_002950"
     CDS             7494..7790
                     /locus_tag="OQQ66_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1905800.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7872..10514
                     /gene="yfcU"
                     /locus_tag="OQQ66_002951"
     CDS             7872..10514
                     /gene="yfcU"
                     /locus_tag="OQQ66_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1905801.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     CTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10534..11286
                     /locus_tag="OQQ66_002952"
     CDS             10534..11286
                     /locus_tag="OQQ66_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1905802.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREISPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11302..11811
                     /locus_tag="OQQ66_002953"
     CDS             11302..11811
                     /locus_tag="OQQ66_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1905803.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11808..12296
                     /locus_tag="OQQ66_002954"
     CDS             11808..12296
                     /locus_tag="OQQ66_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1905804.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12293..12832
                     /locus_tag="OQQ66_002955"
                     /pseudo
     CDS             12293..12832
                     /locus_tag="OQQ66_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3258 a         3082 c         3437 g         3165 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagcggc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actataatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagctgtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgttc
     3361 ggtccggcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgcttg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgtgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgacgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat cggcaactgc aaaacggtgc
     3961 aagaagcgga agctctggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg tggtgggctg gagttggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagcacaac attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagtc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac
     4561 aatcgcaggc gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattgaaaca acagatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg
     4981 cttcgaatac gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc
     5041 aagttatcgg cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc
     5101 ctcatgcggg tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccaaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gtttctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtattggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaatgggcg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa
     6661 taaaacactt tctacattca ttcggaaatt attcttctgc acgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt
     6841 gtttatatat tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt
     6901 gttttttaag ctgttgatat tcattattga tatactttct attttttcta aatacttatt
     6961 ccgtattttt taaggatgtc cttaagataa tctttcttct gtcaatatat taattaaatc
     7021 tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc
     7081 cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca
     7141 atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt
     7201 aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg
     7261 caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg
     7321 cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac
     7381 tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc
     7441 cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tacttaggaa acaatgacca
     7501 ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata
     7561 ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct
     7621 cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct actaataaag
     7681 gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag
     7741 atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc
     7801 catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga
     7861 tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg
     7921 cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt
     7981 cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg
     8041 gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg
     8101 aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc
     8161 tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat
     8221 ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg
     8281 atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc
     8341 ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca
     8401 gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg
     8461 gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta
     8521 actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa
     8581 aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg
     8641 cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat gttggtggta
     8701 gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt
     8761 ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg
     8821 aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta
     8881 cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg
     8941 cctcgatgcc atacctcact cgtccaggtc agattcgcta taagatcatg atgggccgtc
     9001 cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg
     9061 ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg
     9121 cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc
     9181 actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact
     9241 ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg
     9301 gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca
     9361 gcggaatggt acgcacgggc aacgacaaag agatgtgcac cgccacttat aaccagaact
     9421 tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg
     9481 aggagcagat aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta
     9541 atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca
     9601 tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact
     9661 atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc
     9721 actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc
     9781 atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct
     9841 ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc
     9901 gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc
     9961 cggtggaagg taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg
    10021 acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg
    10081 ctgaagcaac ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta
    10141 aatttgctgt cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc
    10201 atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg
    10261 atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga
    10321 gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg
    10381 gcttgttact accatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca
    10441 aaccggcgat tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt
    10501 cagtaaacca gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa
    10561 atcgttgtcc cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg
    10621 gtgacgcccg acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg
    10681 ctgcgcaaca acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag
    10741 ggcaacaaaa tcagctctcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg
    10801 atgaacggcc aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc
    10861 gagagcgtgt tctatttcaa cgtgcgcgaa atttcgccga aatcaaataa accgaacaca
    10921 ctgcagattg cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac
    10981 accagcatga aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc
    11041 accgtcaata acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc
    11101 ggcaacccgc cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg
    11161 aatgcgaaga tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg
    11221 ccgctgtact tcaaatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa
    11281 ggctgatgcc gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg
    11341 ctggccggat ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat
    11401 ctgacgctgc gaatcctagt cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg
    11461 aaatttggca atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa
    11521 tacaccctca actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat
    11581 accgccgtga tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg
    11641 atagaaaacg ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt
    11701 aatgtcaaca ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg
    11761 actccgtcgg agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc
    11821 tgctcctttt actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat
    11881 tccacggtac gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag
    11941 tgcaatttcg cgatctcatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg
    12001 actacgaact gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct
    12061 ggacggggac tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg
    12121 gaattgagct tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg
    12181 ccaccgattt tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg
    12241 tgctgacgga cacgaccttt tctgcttacg caaccttacg ggtggattac caatgaaaac
    12301 agcacacatt tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac
    12361 aggagcacag gcagtcgaca acaacctgca tttttacggc aacctgctga gtaaatcctg
    12421 cacgctggtg gtggatggtg ccaacctggc agaaatccat tttcccaccg tcagcaaccg
    12481 ggatttgatg gtatctgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg
    12541 caaagggccg gcgggataca acgtcaaagt cacgctgagc ggcaccgaag acagcggtca
    12601 gccgggtttt ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga
    12661 aacgctcgac ggcaagcggg tggatattaa caacaccacc ggggcgacct ttgcgcttac
    12721 caacggcaac aatgatatta atttccgggc ctggatctag gcgaaaagcg gtcgtgatgt
    12781 aaccatcggt gatttcaccg ccagcctgac agcaactttt gagtatattt aaaatgaaaa
    12841 tccttcgtca tgttttatgt cttttattgc tgttaatgtt caatacatgc ggccaggcgg
    12901 cgacgaaagt tggtgagtta tatgtgccag ctggtgccag tt
//
LOCUS       DAJVTY010000118        12877 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVTY010000118 DAJVTY010000000
VERSION     DAJVTY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.118"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(14..520)
                     /gene="mug"
                     /locus_tag="OQQ66_002956"
     CDS             complement(14..520)
                     /gene="mug"
                     /locus_tag="OQQ66_002956"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS1905805.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            645..720
                     /locus_tag="OQQ66_002957"
     tRNA            645..720
                     /locus_tag="OQQ66_002957"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ile,seq:cat)
     gene            complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="OQQ66_002958"
     CDS             complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="OQQ66_002958"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS1905806.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1826..2449
                     /gene="nfeR"
                     /locus_tag="OQQ66_002959"
                     /gene_synonym="yqjI"
     CDS             1826..2449
                     /gene="nfeR"
                     /locus_tag="OQQ66_002959"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1905807.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2603..4123)
                     /gene="aer"
                     /locus_tag="OQQ66_002960"
     CDS             complement(2603..4123)
                     /gene="aer"
                     /locus_tag="OQQ66_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS1905808.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4540..5919
                     /gene="ygjG"
                     /locus_tag="OQQ66_002961"
     CDS             4540..5919
                     /gene="ygjG"
                     /locus_tag="OQQ66_002961"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1905809.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPQTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5961..6293)
                     /locus_tag="OQQ66_002962"
     CDS             complement(5961..6293)
                     /locus_tag="OQQ66_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS1905810.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6512..7495
                     /gene="ebgR"
                     /locus_tag="OQQ66_002963"
     CDS             6512..7495
                     /gene="ebgR"
                     /locus_tag="OQQ66_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1905811.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7679..10771
                     /gene="ebgA"
                     /locus_tag="OQQ66_002964"
     CDS             7679..10771
                     /gene="ebgA"
                     /locus_tag="OQQ66_002964"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1905812.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10768..11217
                     /locus_tag="OQQ66_002965"
     CDS             10768..11217
                     /locus_tag="OQQ66_002965"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1905813.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11280..12713
                     /locus_tag="OQQ66_002966"
     CDS             11280..12713
                     /locus_tag="OQQ66_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1905814.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         3105 a         3453 c         3227 g         3092 t
ORIGIN      
        1 tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatacgctt
       61 caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
      121 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg ctgaatccct
      181 gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct tcaatttttt
      241 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
      301 ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct
      361 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
      421 ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
      481 acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc
      541 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcacggta
      601 atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt
      661 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      721 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatct cagttacttt
      781 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
      841 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
      901 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
      961 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
     1021 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
     1081 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
     1141 agcgaaccgc gcggaccagc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag
     1201 ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg
     1261 tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgccc
     1321 tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg
     1381 tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag gacaatgcgc
     1441 tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa gcgcagatca
     1501 ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatggta
     1561 atcagatata tctaaataat tttcgtaaat gataatgatt gttaatcatg ataaatgcaa
     1621 gcgattagta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
     1681 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
     1741 acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa attagatata
     1801 tctaatcaag aaaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga
     1861 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
     1921 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
     1981 tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
     2041 gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa cccaggggaa
     2101 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
     2161 gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
     2221 gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg
     2281 ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
     2341 agcggtgctg gatttacgcg tcaaccagag cgatatcagt gatgcacaaa taaaaaagat
     2401 cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat cgccgcatcc
     2461 gccagtggcg cggtgcaatt gccggatgcg acgcttgacg cgccttatcc ggcctacacc
     2521 cactacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca ttgtgctcca
     2581 accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg
     2641 cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctca gcatttttct
     2701 gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca tcggcctgtt
     2761 ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc tgtgccacaa
     2821 tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc accttatcag
     2881 cactggcatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg ctggctaaat
     2941 gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca cgcgccgctt
     3001 ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta atcagcgaag
     3061 taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg
     3121 tcatcgcctc cccaccctgc actgccgcat tactggcgat gatagaaagt ttatcggcag
     3181 ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg gtcgccaccg
     3241 tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg
     3301 ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg ttaattagcc
     3361 aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc
     3421 gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc tgacgggcga
     3481 cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac aataccacta
     3541 acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg gccgccagca
     3601 agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat gaaggcagtt
     3661 tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg gtacgtccgg
     3721 cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc gcccgggtac
     3781 gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca ttggcccgca
     3841 cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag ggctcccctt
     3901 ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg tgacgcacca
     3961 tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc acaaaagtgt
     4021 cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg gtatcgtccg
     4081 ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg tcctatgcag
     4141 gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa
     4201 ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat tccttgattt
     4261 aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa aatcaggcaa
     4321 ttatcgccct gaaaacgtgc atttgcacag caatcatcaa atccataccc gacaaaaacc
     4381 gtgcaaaata acaacgaatg ttaacaaata gcattaaata ctgcgcaaat gataaccaca
     4441 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     4501 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     4561 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     4621 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     4681 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     4741 tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     4801 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     4861 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     4921 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     4981 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     5041 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     5101 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     5161 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     5221 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     5281 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     5341 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     5401 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     5461 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     5521 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     5581 aatgtgttgc tggagcagaa cttactggct caggctgagc aaaaaggcga tatgttgctg
     5641 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     5701 gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     5761 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     5821 gaaccgccac agacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     5881 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     5941 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     6001 gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     6061 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc
     6121 agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     6181 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     6241 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc
     6301 ttccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa
     6361 aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa
     6421 agaaacgcgc tttatttact gaaaacaggt gacccgataa gtacttcctc tacaatgggg
     6481 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga
     6541 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     6601 gaaagaagag acaaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     6661 tagtgcccgc aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag
     6721 ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     6781 aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca gcggattacc
     6841 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg cccttcgcgc
     6901 cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     6961 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     7021 caatcagggc gttaatcgta ttggttttat tggcggtgaa gatgagccag gcaaagcgga
     7081 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     7141 tatctggcgc ggaggctttt ccagttcgtc gggttatgaa ctggcaaaac agatgctggc
     7201 gcaggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     7261 gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa
     7321 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     7381 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     7441 gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     7501 cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc
     7561 agttaaagta aatgcattta tctgctactt tttagtaaaa attttactaa actccccagc
     7621 aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat
     7681 gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt
     7741 tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt
     7801 tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga
     7861 agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca
     7921 aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt
     7981 gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga
     8041 cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt
     8101 ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga
     8161 catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc
     8221 ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt
     8281 ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga
     8341 cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc tcgccgcctc
     8401 ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag
     8461 cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga
     8521 acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa
     8581 agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa
     8641 agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg
     8701 tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct
     8761 ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc
     8821 gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa ccgacgtcga
     8881 atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga
     8941 aaaagtctac gtcgagcgca ttgttcgcca tatccacgcg cagaaaaacc atccgtcgat
     9001 catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca
     9061 tgcggcgaaa gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga
     9121 agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga
     9181 atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc
     9241 gggcgggctg acggagtacc agaacgtctt ctataagcac gattgcattc agggtcatta
     9301 tgtctgggag tggtgcgacc acgggatcca ggcacaggac gaccacggca atgtctggta
     9361 taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt
     9421 gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc
     9481 ggtaaaaatc cacgcgcggg atctgactcg cggcgagttg aaagtcgaaa ataaactgtg
     9541 gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct
     9601 cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat
     9661 cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc
     9721 ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga
     9781 aaacaccgcg cagccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga
     9841 cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag
     9901 tggcaaaccg acatcctggc aggtaaatgg cgagtcgctg ctgacccgcg agccaaagat
     9961 caacttcttc aagccgatga tcgacaacca caagcaggag tacgaagggt tgtggcagcc
    10021 gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg
    10081 tgaagtgttg atcatcagcc gcacagttat tgcaccgccg gtgtttgact tcgggatgcg
    10141 ctgtacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga
    10201 gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg
    10261 cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca
    10321 gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc
    10381 cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca
    10441 cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac
    10501 ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac
    10561 cctgaacctc gaccaccagc tacttggcct cggctccaac tcctggggca gcgaggtgct
    10621 ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc
    10681 tggcggagaa gctaccgcgc aaagtctggc gtcgtatgag ttcggcgcag ggttcttttc
    10741 cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc
    10801 cagatttacg cctctagcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac
    10861 aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca
    10921 gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac
    10981 ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat
    11041 tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac
    11101 gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg
    11161 gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa
    11221 ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta
    11281 tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgctg
    11341 ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac
    11401 cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat
    11461 ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg
    11521 gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca
    11581 cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata
    11641 ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg
    11701 tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc
    11761 tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc
    11821 ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg
    11881 ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg
    11941 acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg
    12001 gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga
    12061 aatttaccgg cggatcggtg caggtattcc atggcatggc ggcgtatttt ggtctaccgg
    12121 aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc
    12181 tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg
    12241 gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt
    12301 tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc
    12361 tgatgaatac tattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca
    12421 tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg
    12481 gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt
    12541 ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt tataacgtcg
    12601 gcggtattgt tatcttcctt ggctttgcgt ggtggaaata cagtaaatat ataaagggat
    12661 taacggctga agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat
    12721 aataaaaaat gagcaggcat tttccctctt cctggatgag gagagggaaa atagtttctg
    12781 cctttatatt tttataatta caacgataaa aggctgtact ttttctttag ctcatggatt
    12841 aacacaatga aattaatcac tgcaccatgc agagcat
//
LOCUS       DAJVTY010000119        12866 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVTY010000119 DAJVTY010000000
VERSION     DAJVTY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12866
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.119"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..850)
                     /gene="rarD"
                     /locus_tag="OQQ66_002967"
     CDS             complement(<1..850)
                     /gene="rarD"
                     /locus_tag="OQQ66_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1905815.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIF"
     gene            complement(902..1369)
                     /locus_tag="OQQ66_002968"
     CDS             complement(902..1369)
                     /locus_tag="OQQ66_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1905816.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1534..2403
                     /gene="pldA"
                     /locus_tag="OQQ66_002969"
     CDS             1534..2403
                     /gene="pldA"
                     /locus_tag="OQQ66_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1905817.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2536..4365
                     /gene="recQ"
                     /locus_tag="OQQ66_002970"
     CDS             2536..4365
                     /gene="recQ"
                     /locus_tag="OQQ66_002970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1905818.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4429..5049
                     /gene="rhtC"
                     /locus_tag="OQQ66_002971"
     CDS             4429..5049
                     /gene="rhtC"
                     /locus_tag="OQQ66_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1905819.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5111..5731)
                     /gene="rhtB"
                     /locus_tag="OQQ66_002972"
     CDS             complement(5111..5731)
                     /gene="rhtB"
                     /locus_tag="OQQ66_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1905820.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5842..6864
                     /gene="pldB"
                     /locus_tag="OQQ66_002973"
     CDS             5842..6864
                     /gene="pldB"
                     /locus_tag="OQQ66_002973"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1905821.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6872..7672
                     /gene="yigL"
                     /locus_tag="OQQ66_002974"
     CDS             6872..7672
                     /gene="yigL"
                     /locus_tag="OQQ66_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1905822.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7748..8647
                     /gene="bioP"
                     /locus_tag="OQQ66_002975"
     CDS             7748..8647
                     /gene="bioP"
                     /locus_tag="OQQ66_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1905823.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8535..9488)
                     /gene="metR"
                     /locus_tag="OQQ66_002976"
     CDS             complement(8535..9488)
                     /gene="metR"
                     /locus_tag="OQQ66_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1905824.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9724..11985
                     /gene="metE"
                     /locus_tag="OQQ66_002977"
     CDS             9724..11985
                     /gene="metE"
                     /locus_tag="OQQ66_002977"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1905825.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12270..12515)
                     /locus_tag="OQQ66_002978"
     CDS             complement(12270..12515)
                     /locus_tag="OQQ66_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1905826.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12505..>12866)
                     /locus_tag="OQQ66_002979"
     CDS             complement(12505..>12866)
                     /locus_tag="OQQ66_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905827.1"
                     /translation="GGPIENLTTDGVTINREGIAIVEKHIARFGHDPINEVMINRLKD
                     IEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTATLEDYKL
                     KGELNDLYHQDALDYDN"
BASE COUNT         3037 a         3250 c         3432 g         3147 t
ORIGIN      
        1 caaaaattgc cagcgccacc caaataaagg cgaaggtcac catcttatcg gcacccggtt
       61 tttcaccata aaacgtcact gccaacagga acatcagcgt cgggccaata tactggaaga
      121 agcctaacgt ggagagacgc aggcgtgtag cagctgcggt aaaacacaac agcggtacgg
      181 tggtgacaat accggcggcg atcagcagca aattcagcaa catcgggttt tgccccatat
      241 ggctggttga gctgtcggca atagcaaaca gataaatcgc cgccacgggc agcagccaca
      301 tggtttcgat taacatgccg gtttgcgctt caacggcaat tttcttgcgt accagtccgt
      361 agaaggcaaa actaaacgcc agccccagcg cgatgattgg cagcgaacca aaagtccaca
      421 actgaaccaa cacgccacat atcgccagaa tcaccgccag ccattgcatc cggcggaatc
      481 gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata aagtaaccaa
      541 ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc cagttgccac
      601 caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga atcagcgttt
      661 ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa aacgaccaga
      721 tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagcttg aagtatgctg
      781 gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt
      841 ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg cttttatgtc
      901 ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct tcattgtgta
      961 attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg gtagcagtaa
     1021 aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc cccatccgcg
     1081 atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag gtacttccca
     1141 cgcacaccag accggcggcg acatccagcg ccgacgcaat gactccgccg tgcaaaatgc
     1201 tttgcgccca gttacccacc atcatcggct gatttttaaa ggccagctgt gcgaactctt
     1261 tttcgtaacg ctccagctcc atccctaacg cgcggttaaa tggcatgtga taaacaaaca
     1321 tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata tcatccttac
     1381 acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt ctactttagg
     1441 aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa aaacattcac
     1501 cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg ctggttgttg
     1561 ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa agaggtgcat
     1621 gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca tgacaatccg
     1681 ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag cgatctgaac
     1741 aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga agtaaagttt
     1801 cagttgagcc tggcatttcc actgtggcgt gggattttag gcccgaactc ggtgttgggt
     1861 gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc ttcaccgttt
     1921 cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta ccgttttgca
     1981 ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg gcgttccgac
     2041 ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa cggtaactgg
     2101 ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc ggatatcacc
     2161 aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc ggtgttgagt
     2221 gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg cttaagttac
     2281 ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg cgaatcgctc
     2341 atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa cgacttgttt
     2401 tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta ccctctcaga
     2461 gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt tttatttcag
     2521 gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg agctaaacag
     2581 gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga aattatcgac
     2641 actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg aaaatccctt
     2701 tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc accgctgatt
     2761 tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc ggcgtgcctt
     2821 aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg caccgggcaa
     2881 attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct tgagcatctg
     2941 gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc ccaatggggc
     3001 cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt cccgaccctg
     3061 ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat cgtgcgcttg
     3121 ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa tattcgctac
     3181 atgctgatgg agaagttcaa accgctcgat cagttgatgc gctacgtgca ggaacagcgc
     3241 ggtaagtcag gcattatcta ctgcaacagc cgcgcgaaag tagaagacac cgctgcgcgc
     3301 ctgcaaagca agggaattag cgcggcggcc tatcatgctg ggctggaaaa taatgttcgc
     3361 gccgacgtgc aggaaaaatt tcagcgcgat gacctgcaaa ttgtggtggc gacggtggcg
     3421 ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga tattccgcgc
     3481 aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct gcccgcggaa
     3541 gcgatgctgt tttacgatcc ggccgatatg gcgtggctgc gccgttgcct ggaagagaag
     3601 ccgcaggggc agttacagga tatcgaacgt cataaactca atgcgatggg cgcttttgcc
     3661 gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg gcggcaagag
     3721 ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg ttcaaccgat
     3781 gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat gggttatgtg
     3841 gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca tgacaaactg
     3901 aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt gatccgccag
     3961 ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct acaactgaca
     4021 gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt gccgcgtatc
     4081 gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga tcgcaaactg
     4141 ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt cccgccgtac
     4201 gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat caccgccagc
     4261 gaaatgctca gcgttaacgg cgttgggatg tgcaagctgg aacgctttgg taaaccgttt
     4321 atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc agcataaaaa
     4381 agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat gttgatgtta
     4441 tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc cgatttcttt
     4501 tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg cgtgctgggt
     4561 attacctgcg gcgttatggt ttgggcaggg attgcgttgc ttggcctgca tttgattatc
     4621 gaaaaaatgg cctggctgca tacgctgatt atggttggcg gtggcctgta tctctgctgg
     4681 atgggttacc agatgctacg cggtgcactg aaaaaagagg ctgtatctgc gcctgcacca
     4741 cagattgagc tggcgaaaag cgggcgcagt ttcctgaaag gtttactgac caatctcgct
     4801 aatccgaaag cgattatcta ctttggctcg gtgttctcat tgtttgtcgg tgataacgtt
     4861 ggcactaccg cgcgctgggg catttttgcg ctgatcattg tcgaaacact ggcgtggttt
     4921 accgtcgttg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca acgtctggcg
     4981 aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca tttgattatt
     5041 tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac gccgcatccg
     5101 acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc ataaacaacg
     5161 agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat agagcaatcc
     5221 gttgagcaag ggtggcgtaa ccgatcatca caataatatc gactacaata gtggtgacgc
     5281 cgagcacgat atactgcatc agttgcggct gctgtggcat gatgaattgc ggaaatagcg
     5341 ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg cgctggaaca
     5401 aatgtcgacg cgattgagta gaggccagcg atttaaggtc aattgcacca gcggcgcgcc
     5461 actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac acttcaaacg
     5521 caatcactga gcgggaaaat agcgtcccca aacccacgcc aaccagcaca atatgaatcg
     5581 ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg tggttgagcg
     5641 aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg atcgatgtca
     5701 gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc tatttttgtg
     5761 ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg attttacgtg
     5821 gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac aagagaaaac
     5881 gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg tgatgaagca
     5941 gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc acagcaccat
     6001 gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata tgcggaactg
     6061 gcctatgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg cgggcaggga
     6121 cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt taatgattat
     6181 gttgatgatc tggcggcatt ctggcagcag gaggtccagc ccggtccgtg gcgtaaacgc
     6241 tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca acgccatcca
     6301 ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat tcgtatgccg
     6361 tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt ccgtgatggc
     6421 tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt actgacccac
     6481 agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac gattcgcgtc
     6541 ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca ggtgttagcc
     6601 ggtgcgggtg atgacgccac gccaacgctt ctcttgcagg ctgaagagga acgcgtggtg
     6661 gataaccgca tgcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca tcctgtcgaa
     6721 ggaggacggc cgttggtaat taaaggtgct taccatgaga tcctttttga aaaggacgca
     6781 atgcgctcag tcgcgctcca cgccatcgtt gattttttca acaggcataa ctcacccagc
     6841 ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt ctgatttaga
     6901 tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa ctctgaagct
     6961 gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg ttgatgtggg
     7021 gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg gtgcgcgcgt
     7081 tcacgatctg gatggtaatc tgatttttgc tcataacctg gatcgcgaca ttgccagcga
     7141 tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc gcgacgacga
     7201 atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag cggtgttcaa
     7261 atatgcgctg tatgagcctg gattactgga gccggaaggc gtcagcaaag tgttcttcac
     7321 ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc gttggggcga
     7381 tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg gcggcgtttc
     7441 aaaaggccat gcgctggaag cggtggcgaa gaaactgggc tacagcctga aggattgtat
     7501 tgcgtttggt gacgggatga acgacgccga aatgctgtcg atggcgggga aaggctgcat
     7561 tatgggcagt gcgcaccagc gtctgaaaga ccttcatccc gagctggaag tgattggtac
     7621 taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt aatcgttctt
     7681 tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa tcaaagagtt
     7741 ttccattgtg gcgctactta tcatcaccac gattctgtgg gccttctcct ttagctttta
     7801 tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc gcgttggcct
     7861 ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga aaacagtcgg
     7921 cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga gtttccgcgc
     7981 ttatctctat ctgacggttt ccgaactgct gctgttcacc gtactgacgc cgctctacat
     8041 cacgctgatt tatgacatca tgaataagcg ccgtctgcgc tggggatatg cttttagtgc
     8101 cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg accatttctg
     8161 gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca tggtgggtta
     8221 caaacgcctg atggaaactc gcccgatgcc gcagcataac gcctttgcgt ggttctatct
     8281 tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc agaaaatgcc
     8341 gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt ccgggattgg
     8401 ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg gaattatgaa
     8461 taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc aacagccgca
     8521 ctggccaacg ttgattacag gcgcgctggt gatcctggcc tcactgtggg tgcatcgtaa
     8581 gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt gcgcgctgag
     8641 cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc ggcgtacagt
     8701 cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg ctcaaaactc
     8761 tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat ctgaatcaac
     8821 aataaggtgt tatcgacgct tttcagtgac ggactgacgc ctgccggctg aagaaaatgc
     8881 cgccagacat ccagtcgact acgctgcacc ggataaatta atagcgtctc gctggcgaga
     8941 tcttccggtg taatacgcgt ttttgccgcc agtggatggt caggtgctaa caccagacgc
     9001 acttcatagt cgaacatcgg cgaataatgc agaccactgc gcggcagaat atcggacgtc
     9061 attaccagat ccagctctcc ctgttgcaag gcgggctgcg ggtcaaatgt cacgcccgat
     9121 ttaaagtcca tctcgacctg cggccagttc ttatggaaat tttctaacgc gggcgtcagc
     9181 cactgtatac agctatggca ctcaatggca atgcgcagac gcgtctgctg cggttcattg
     9241 caggcttgca gtgcctggct aatttgtggc agcacctggt ttgccagttg caacaggatt
     9301 tctccctgcg gtgtaaagcg aagcggctgg cttttacgca caaatagccg gaagccaagg
     9361 cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtttgatg caacgtcgcc
     9421 gcagcggctg cgagtgagcc gcagttccgc aacgcttgta gcgttttcag atgttttact
     9481 tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa tatacagtaa
     9541 ccgccaatta tggatgtgta aacatctgga cggctaaaat ccttcgtctt ttaaatttat
     9601 ggtgcgttgg ctgcgtttct ccaccccggt cacttacttc agtaagctcc cggggatgaa
     9661 taaacttgcc gccttcccta aattcaaaat ccataggatt tacatataat tagaggaaga
     9721 aaaatgacaa tattgaatca caccctcggt ttccctcgcg ttggcctgcg tcgcgagctg
     9781 aaaaaagcgc aagagagtta ttgggcaggg aactccacgc gtgaagaact gctggcggta
     9841 gggcgtgaat tgcgtgctcg tcactgggat caacaaaagc aagcgggtat cgacctgctg
     9901 ccggtgggcg attttgcctg gtacgatcat gtactgacca ccagtctgct gctgggtaac
     9961 gttccggcgc gtcatcagaa caaagatggt tcggtagata ttgacaccct gttccgtatt
    10021 ggtcgtggac gtgcgccgac tggcgaacct gcggcggcag cggaaatgac caaatggttt
    10081 aacaccaact atcactacat ggtgccggaa ttcgttaaag gccaacagtt caaactgacc
    10141 tggacgcagc tgctggagga agtggacgag gcgctggcgc tgggccacaa cgttaagcct
    10201 gtgctgctgg ggccggttac ctggctgtgg ctggggaaag tgaaaggtga acagtttgac
    10261 cgcctgagcc tgctgaacga cattctgccg gtttatcagc aagtgctggc agaactggag
    10321 aaacgcggca tcgagtgggt acagattgat gaacccgcgc tggtactgga actaccacag
    10381 gcgtggctgg acgcatacaa acccgcttac gacgcgctcc agggacaggt gaaactgctg
    10441 ctgactacct attttgaagg cgttacgcca aatctcgaca cgattactgc gctgcctgtt
    10501 cagggactgc atgtggacct tgtacatggt aaagatggcg ttgctgaact gcacaagcgc
    10561 ctgccttctg actggctgct gtctgcggga ctgatcaatg gtcgtaacgt ctggcgcgcc
    10621 gatcttactg agaaatatgc gcaaattaag gacattgtcg gcaaacgtga tttgtgggtg
    10681 gcatcttcct gctcgttgct gcacagcccc atcgacctga gcgtggaaac gcgtcttgat
    10741 gcagaagtga aaagctggtt tgccttcgcc ttacaaaaat gccatgaact ggcactgctg
    10801 cgcgatgcgc tgaacagtgg tgacacggca gctctggcag agtggagcgc cccaattcag
    10861 gcgcgtcgtc actctactcg tgtacataat ccggcggtag aaaagcgtct ggcggcgatc
    10921 accgcccagg acagccagcg tgcgaatgtc tatgaagtgc gtgctgaagc ccagcgtgcg
    10981 cgttttaaac tgccagcgtg gccgaccacc acgattggtt ccttcccgca aaccacggaa
    11041 attcgtaccc tgcgtctgga tttcaaaaag ggcaatctcg acgccaacaa ctaccgcacg
    11101 ggcattgcgg aacatatcaa gcaggccatt gttgagcagg aacgtttggg actggatgtg
    11161 ctggtacatg gcgaggccga gcgtaatgac atggtggaat actttggcga gcacctcgac
    11221 ggatttgtct ttacgcaaaa cggttgggta cagagctacg gttcccgctg cgtgaagcca
    11281 ccgattgtta ttggtgacgt tagtcgcccg gcaccgatta ccgtggagtg ggcgaagtat
    11341 gcgcaatctc tgactgataa accggtgaaa gggatgctga ctggcccggt gaccattctc
    11401 tgctggtcgt tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca aattgcgctg
    11461 gcgctgcgtg atgaagtggc ggacctggaa gccgcaggaa ttggcatcat ccagattgat
    11521 gaaccggcgc tgcgcgaagg tttaccgctg cgtcgtagcg actgggatgc atatctccag
    11581 tggggcgtgg aggccttccg tatcaacgcc gccgtggcga aagatgacac acaaatccac
    11641 actcacatgt gttattgcga gttcaacgac atcatggatt ccattgcggc actggatgca
    11701 gacgtcatca ccatcgaaac ctcgcgttcc gacatggagt tgctggagtc gtttgaagag
    11761 tttgattatc cgaatgaaat cggtccaggc gtctatgaca ttcactcgcc aaacgtaccg
    11821 agcgtagaat ggattgaagc cttgctgaag aaagcggcaa aacgcattcc ggcagagcgc
    11881 ctgtgggtca acccggactg tggcctgaaa acgcgcggct ggccagaaac ccgcgcggca
    11941 ctggcgaaca tggtgcaggc ggcgcagaat ttgcgtcggg gatgatgaat attaacgagt
    12001 cactgtaaat tgcagtacga tgaaagaggc aaaactgcct gataccaggc ctacataatt
    12061 tatgcaattt attgaatttt tgagttttgt tggccggaca aggcgttcac gccgcatccg
    12121 gcatgaacaa cgcgcacttt gccaataatc tgatggcttc catcaccgga agccattttg
    12181 ctatctgcgc ccgttcagat acagaaataa ccgaatcatt tttacgcaaa atataatcct
    12241 gagcgatttg ttcagtaatc aacgctacgt tatttattct cacttttcac gaaattgtat
    12301 cccttttcag taacgtggta gtgaggcata gacttatgtc cgctatcctc aataataacc
    12361 aaaccctcgt tagctaattt gttaacaatg ttaacaacat taccgaatcc tggaatttgc
    12421 cttatggata gtgtgcgatc gagattcatc cagttccagt ttatatcttt ctcagccata
    12481 actttcagta cgttaatttc agtttcagtt atcatagtca agtgcatcct ggtggtataa
    12541 gtcattaagt tctcccttaa gtttataatc ttctagagta gcagtatgcg tatgattcca
    12601 gagtgcatag cctgcgtcac catcaggttg tcctgtttcc caaccaagat tacagtaacg
    12661 ctgatattcc cgacactcat gagtatagaa gttaagatct acttgttctg gggggatttt
    12721 tcctttctca atgtctttca gtctattgat catcacttca tttatagggt catgaccaaa
    12781 tctggctatg tgtttttcaa caatagcgat accttccctg ttgattgtta cgccatctgt
    12841 cgtcagattt tctattggac caccgg
//
LOCUS       DAJVTY010000120        12828 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVTY010000120 DAJVTY010000000
VERSION     DAJVTY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.120"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            100..1074
                     /gene="cysB"
                     /locus_tag="OQQ66_002980"
     CDS             100..1074
                     /gene="cysB"
                     /locus_tag="OQQ66_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1905828.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1369..1533
                     /locus_tag="OQQ66_002981"
     CDS             1369..1533
                     /locus_tag="OQQ66_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1905829.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1536..1703
                     /locus_tag="OQQ66_002982"
     CDS             1536..1703
                     /locus_tag="OQQ66_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905830.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1817..1912
                     /locus_tag="OQQ66_002983"
     CDS             1817..1912
                     /locus_tag="OQQ66_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905831.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2076..4751
                     /gene="acnA"
                     /locus_tag="OQQ66_002984"
                     /pseudo
     CDS             2076..4751
                     /gene="acnA"
                     /locus_tag="OQQ66_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4815..5405)
                     /gene="ribA"
                     /locus_tag="OQQ66_002985"
     CDS             complement(4815..5405)
                     /gene="ribA"
                     /locus_tag="OQQ66_002985"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1905832.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5575..6339
                     /gene="pgpB"
                     /locus_tag="OQQ66_002986"
     CDS             5575..6339
                     /gene="pgpB"
                     /locus_tag="OQQ66_002986"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1905833.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6488..6796
                     /gene="lapA"
                     /locus_tag="OQQ66_002987"
     CDS             6488..6796
                     /gene="lapA"
                     /locus_tag="OQQ66_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1905834.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6803..7972
                     /gene="lapB"
                     /locus_tag="OQQ66_002988"
     CDS             6803..7972
                     /gene="lapB"
                     /locus_tag="OQQ66_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1905835.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8165..8902
                     /gene="pyrF"
                     /locus_tag="OQQ66_002989"
     CDS             8165..8902
                     /gene="pyrF"
                     /locus_tag="OQQ66_002989"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1905836.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8902..9228
                     /gene="yciH"
                     /locus_tag="OQQ66_002990"
     CDS             8902..9228
                     /gene="yciH"
                     /locus_tag="OQQ66_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1905837.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9354..9572)
                     /gene="osmB"
                     /locus_tag="OQQ66_002991"
     CDS             complement(9354..9572)
                     /gene="osmB"
                     /locus_tag="OQQ66_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1905838.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9841..10590)
                     /locus_tag="OQQ66_002992"
     CDS             complement(9841..10590)
                     /locus_tag="OQQ66_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1905839.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10680..10853)
                     /locus_tag="OQQ66_002993"
     CDS             complement(10680..10853)
                     /locus_tag="OQQ66_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905840.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11000..>12828)
                     /gene="pdeR"
                     /locus_tag="OQQ66_002994"
     CDS             complement(11000..>12828)
                     /gene="pdeR"
                     /locus_tag="OQQ66_002994"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1905841.1"
                     /translation="IREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWH
                     DTPAVARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQS
                     VFKLFMSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSE
                     IFLICSGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVY
                     LDLDNFKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSA
                     LEAMASRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEG
                     GRGQFCVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRW
                     QSPERGLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQ
                     LADQTIFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDD
                     FGTGYSSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGV
                     ESAKEDAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3258 a         3026 c         3235 g         3309 t
ORIGIN      
        1 gttatagtta gcaccttttt tattattaaa tcgtattagt cacccgccag gtgtgacgaa
       61 aaaacgatgt tctgatggcg tctaagtgga tggtttaaca tgaaattaca acaacttcgc
      121 tatattgttg aggtggtcaa tcataacctg aatgtctcat caacagcgga aggactttac
      181 acatcacaac ccgggatcag taaacaagtc agaatgctgg aagacgagct aggcattcaa
      241 attttttccc gaagcggcaa gcacctgacg caggtaacgc cagcagggca agaaataatt
      301 cgtatcgctc gcgaagtcct gtcgaaagtc gatgccataa aatcggttgc cggagagcac
      361 acctggccgg ataaaggctc actgtatatc gccaccacgc atacccaggc acgctacgca
      421 ttaccaaacg tcatcaaagg ctttattgag cgttatcctc gcgtttcttt gcatatgcac
      481 cagggctcgc cgacacaaat tgctgatgcc gtctctaaag gcaatgccga tttcgctatc
      541 gccacagaag cgctgcatct gtatgaagat ttagtgatgt taccgtgcta ccactggaat
      601 cgggctattg tagtcactcc ggatcacccg ctggcaggca aaaaagccat taccattgaa
      661 gaactggcgc aatatccgtt ggtgacatat accttcggct ttaccggacg ttcagaactg
      721 gatactgcct ttaaccgcgc agggttaacg ccgcgtatcg ttttcacggc aacggatgct
      781 gacgtcatta aaacttacgt ccggttaggg ctgggggtag gggtcattgc cagcatggcg
      841 gtggatccgg tcgccgatcc ggaccttgtg cgcgttgatg ctcacgatat cttcagccac
      901 agtacaacca aaattggttt tcgccgtagt acttttttgc gcagttatat gtatgatttc
      961 attcagcgtt ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc
     1021 tctaatgaag aaattgaggt catgtttaaa gatataaaac tgccggaaaa ataatttctt
     1081 gcgttatttt cggcaccttt tatgtagcga aggtgccgga atatattctc ttttgttact
     1141 tatttttata ccgtgcacaa tatttttatc cctcatattt atagggtaaa ttacctgtaa
     1201 agtagtgtct aattacccgc ctcgcaaatt ccagttttca aatatctatt tcaggtcaaa
     1261 agattgaatt attcactgga gacgattcgt aaattcgctg gatttttgac taaagtttct
     1321 ttaggatttg tctcatcgta tgattaatta caccaatcgt tcaacgtctt gctgataagt
     1381 gatggcgatt atatgaggtt agcaatgcct tctggaaatc aggaaccccg ccgcgatcct
     1441 gaattaaaac gtaaagcctg gctggcggtt tttcttggtt ctgcactttt ctgggtggtt
     1501 gtcgcactgc tgatttggaa agtgtgggga taactatggt cggtcaggag caactggagt
     1561 cgtcaccatt atgccagcat agtgacaatg aaacagaaac gaaacgggaa tgttccgtcg
     1621 ttattccaga cgactggcaa ctaacatcgc agcagcaagc ctttatagaa ctgtttgctg
     1681 aagatgatca gccgaaacaa taattatcat cattcttatt acccattttt aatgaattaa
     1741 agggctttta atacaccgca gcaataacag cttgagttat ctcaacacaa aataataacc
     1801 gttaagggtg tagcctatga tcaacacaaa tatgaaatat tggtcctgga tgggcgcgtt
     1861 ttctctgtcg atgctcttct gggccgaact cctctggatc attactcact gatccttgac
     1921 cccgctgcgg cggggttgtc atttgctttg ccacaaggtt tctcctcttt tatcaatttg
     1981 ggttgttatc aaatcgttac gcgatgtttg tgttatcttt aatattcacc ctgaagagaa
     2041 tcagggcttc gcaaccctgt cattaaggag gagctatgtc gtcaacccta cgagaagcca
     2101 gtaaggacac gttgcaggcc aaagataaaa cttaccacta ctacagcctg ccgcttgctg
     2161 ctaaatcact gggcgatatc acccgtctac ccaagtcact caaagttttg ctcgaaaacc
     2221 tgctgcgctg gcaggatggt aactcggtta ccgaagagga tatccacgcg ctggcaggat
     2281 ggctgaaaaa tgcccatgct gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgt
     2341 aggactttac cggcgtacct gccgttgttg atctggcggc aatgcgcgaa gcggttaaac
     2401 gcctcggcgg cgatactgca aaggttaacc cgctctcacc ggtcgacctg gtcattgacc
     2461 actcggtgac cgtcgatcgt tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg
     2521 aaatggagcg caaccacgaa cgttatgtgt tcctgaaatg gggaaagcaa gcgttcagtc
     2581 ggtttagcgt cgtgccgcca ggcacaggca tttgccatca ggttaacctc gaatatctcg
     2641 gcaaagcagt gtggagtgaa ttgcaggacg gtgaatggat tgcttatccg gatacactcg
     2701 ttggtactga ctcgcacacc actatgatca acggccttgg cgtgctgggg tggggcgtgg
     2761 gtgggatcga agcagaagcc gcaatgttag gccagccggt ttccatgctt atcccggacg
     2821 tagtgggctt taaacttacc ggtaaattac gtgaaggtat taccgccaca gacctggttc
     2881 tcactgttac ccaaatgctg cgcaaacatg gcgtggtggg gaaattcgtc gaattttatg
     2941 gtgatggtct ggattcacta ccgctggcgg atcgcgccac cattgccaat atgtcgccag
     3001 aatatggtgc cacctgtggc ttcttcccaa tcgatgctgt aaccctcgat tacatgcgtt
     3061 taagcgggcg cagcgaagat caggttgagt tggtcgaaaa atatgccaaa gcgcagggca
     3121 tgtggcgtaa cccgggcgat gaaccgattt ttaccagtac gttagaactg gatatgaatg
     3181 acgttgaagc gagcctggca gggccaaaac gcccacagga tcgcgttgca ctgcccgatg
     3241 taccaaaagc atttgccgcc agtaacgaac tggaagtgaa tgccacgcat aaagatcgcc
     3301 agccggtcga ttatgttatg aacggacatc agtatcagtt acctgatggc gctgtggtca
     3361 ttgctgcgat aacctcgtgc accaacacct ctaacccaag tgtgctgatg gccgcaggct
     3421 tgctggcgaa aaaagccgta actctgggcc tcaagcggca accgtgggtc aaagcgtcgc
     3481 tggcaccggg ttcgaaagtc gtttctgatt atctggcaaa agcgaaactg acaccgtatc
     3541 tcgacgaact ggggtttaac cttgtgggat acggttgtac cacctgtatt ggtaactctg
     3601 ggccgctgcc cgatcctatc gaaacggcaa tcaaaaaagg cgatttaacc gtcggtgcgg
     3661 tgctgtccgg caaccgtaac tttgaaggcc gtatccatcc gctggttaaa actaactggc
     3721 tggcctcgcc gccgctggtg gttgcctatg cgctggcggg aaatatgaat atcaacctgg
     3781 ctgctgagcc tattggtcat gatcgtaaag gcgacccggt ttatctgaaa gatatctggc
     3841 catcggcaca ggaaattgcc cgtgcggtag aacaagtctc cacagaaatg ttccgtaaag
     3901 agtacgcaga agtttttgaa ggcacagcag agtggaagga aattaacgtc acacgatccg
     3961 atacctacgg ttggcaggag gattcaacct atattcgctt atcgcctttc tttgatgaaa
     4021 tgcaggcaac accagcacct gtggaagata ttcacggtgc gcggatcctc gcaatgctgg
     4081 gggattcagt caccactgac catatctctc cggcgggcag tattaagccc gacagcccgg
     4141 cgggtcgata tctacaaggt cggggtgttg agcgaaaaga ctttaactcc tacggttcgc
     4201 ggcgtggtaa ccatgaagtg atgatgcgcg gcaccttcgc caatattcgc atccgtaatg
     4261 aaatggtgcc tggcgttgaa ggggggatga cgcggcattt acctgacagc gatgttgtct
     4321 ctatctatga tgccgcgatg cgctataagc aggagcaaac gccgctggcg gtgattgccg
     4381 ggaaagagta tggatcaggc tccagccgtg actggacggc aaaaggtccg cgtctgcttg
     4441 gtattcgtgt ggtgattgcc gaatcgtttg aacgaattca ccgttcgaat ttaattggca
     4501 tgggcatctt gccgctggaa tttccgcaag gcgtgacgcg taaaacgtta gggctaaccg
     4561 gggaagagaa gattgatatt ggcgacctgc aaaatctaca acccggcgcg acggttccgg
     4621 tgacgcttac gcgcgcggat ggtagccagg aagtcgtacc ctgccgttgt cgtatcgaca
     4681 ccgcgacgga gttgacctac taccagaacg acggcatttt gcattatgtc attcgtaata
     4741 tgctgaagta acaagtattt gcttgccggt tattttgctt ccggcaagca aattaattcc
     4801 acaatgcgag agggttattt gttcagcaaa tgacccattt tctcggcttt ggtatcgaga
     4861 taatgttcgt tattggggtt acgacctaca atcaatggta cgcgttcaac aatattaatt
     4921 cctgcttcgg tcagaatttc gacttttttc gggttattgg ttaacaagcg gacttcattg
     4981 acgccaagga gtttgaacat atcagcgcaa agagtgaagt cgcgctcatc agcggcgaag
     5041 cctaactggt ggttagcctc tacggtatcg taaccttgat cctgcagtgc gtaagcgcgg
     5101 attttattca gcagaccaat gttacgacct tcctgacggt gatacagcaa aataccacgg
     5161 ccttcctcgg caatttgcgt caatgccgct tcgagctgga agccacaatc gcagcgcaag
     5221 ctgaacaggg cgtcaccggt cagacattcg gaatggacgc gcgcaagtac cggggtatgc
     5281 ccggaaatat cgccatagac tagcgcgaca tgatcgtgtc cggttgccag ttcttcaaat
     5341 cccaccatca ggaaatcgcc ccatggggtt ggcagtttgg cttctgccac acgtttaagc
     5401 tgcatgaaat tctccagata atgctggttc tgtattggct tattttgcca taacgagaag
     5461 gggttcacct aatcacgacg cgtcgatcgt tcacggaatg gcacaaatct gtcaattttt
     5521 cctggaactg gcgttttcag ttatgattgt gggacttatc aaaaaggaga ggccatgcgt
     5581 tcgattgcca gacgtaccgc agtgggagct gcactattgc ttgtcatgcc agtagccgta
     5641 tggatttctg gctggcgttg gcaacctgga gaacaaagtt ggctactaaa agcggctttt
     5701 tgggttactg aaactgtcac ccagccctgg ggcgtcatta cacatttgat tttattcggc
     5761 tggtttctct ggtgtctgcg ttttcgcatt aaggctgcct ttgtattatt tgccattctg
     5821 gcggccgcaa tccttgtggg acaaggcgtt aaatcctgga tcaaagacaa agtccaggaa
     5881 ccacgacctt ttgttatctg gctggaaaaa acacatcata ttccggttga tgagttctac
     5941 actttaaagc gagcagaacg cggaaatcta gtgaaagaac agttggctga agagaaaaat
     6001 atcccacaat atttgcgttc acactggcag aaagagacgg ggtttgcctt tccttccggt
     6061 cacacgatgt ttgctgccag ttgggcactg ctggccgttg gtttgctgtg gccgcgtcgg
     6121 cgaacgttaa ccattgctat cttgctgctc tgggcaacgg gagtcatggg aagccgcctg
     6181 ctgctcggga tgcattggcc acgcgatctg gtagtagcta cattgatttc gtgggcgctg
     6241 gtggcggtgg caacgtggct tgcgcaacga atttgtgggc cattaacacc acctgcggaa
     6301 gaaaatcgcg aaatagcgca acgagaacaa gaaagttaac gctggttgat tttccgaatt
     6361 tagcccttaa atcatcaaca atgcgcgtgg atgccatttc gcagacggcg cgaaaatggt
     6421 actttaaagg gctattgcgg taagttgacc ataatttatt cgctctaacc acataacggg
     6481 aagtaatgtg aaatatttac tcattttctt actggtgtta gcgatcttcg tgatttcggt
     6541 cacgttgggt gcgcagaacg atcaacaggt gacgtttaat tatctgttag cgcaagggga
     6601 gtaccgtatt tccacattgc tggcggtatt gtttgctgcg gggtttgcta tcggttggtt
     6661 gatttgtggc ctgttctggc tgcgagttcg tgtttccctg gcgcgcgctg aacgtaaaat
     6721 aaagcgactg gaaaaccagc tttcacccgc gactgacgtg gctgtagtgc cgcactcgtc
     6781 agcggcgaag gaataacttt ctatgctgga gttgttgttt ctgcttttgc ctgtagccgc
     6841 tgcctatggc tggtatatgg gccgcagaag tgcgcaacaa aacaagcaag atgaagccaa
     6901 ccgcttgtcg cgtgattacg tagcgggggt taacttcctg cttagtaatc aacaggataa
     6961 agcggtagac ctgtttctcg atatgcttaa agaggataca ggcaccgttg aagcccacct
     7021 tacgctcgga aacctgttcc gttcgcgtgg cgaagttgat cgcgctattc gcatccatca
     7081 gaccctaatg gaaagcgcct cgctgaccta tgaacagcgt ctgttggcga ttcaacaact
     7141 agggcgtgat tacatggccg ccgggttata cgaccgcgcg gaagacatgt tcaatcagct
     7201 gaccgatgaa actgacttcc gcattggcgc tctgcaacag ttgctacaaa tctaccaggc
     7261 taccagtgag tggcagaaag caattgatgt tgccgaacgc ctggtgaagc tgggtaaaga
     7321 taaacagcgc gtcgaaattg cccatttcta ctgtgagtta gccctgcagc atatggccag
     7381 cgacgatctc gatcgtgcca tgaccttgct aaaaaaaggg gcggcggcag ataaaaacag
     7441 agcccgcgta tccatcatga tgggacgcgt gtttatggcg aaaggagaat acgccaaagc
     7501 cgtcgaaagt ttgcaacgcg tgatatccca ggacagagaa ctggtcagcg aaacgctgga
     7561 aatgttgcaa acctgctatc agcagttggg taaaactgcc gaatgggcag aattcctgca
     7621 acgcgcggtg gaagagaaca ccggtgccga tgctgaattg atgcttgctg atatcatcga
     7681 agcgcgcgac ggtagtgagg ccgcacaggt ctatattacg cgccagcttc agcgtcatcc
     7741 gaccatgcgt gtgttccata agttaatgga ttaccactta aatgaagcgg aagaagggcg
     7801 tgccaaagag agcctgatgg tgctgcgtga catggttggc gaaaaggtac gtagtaagcc
     7861 tcgttatcgc tgccagaaat gtggttttac cgcatacacc ctctactggc attgtccgtc
     7921 ttgtcgggcc tggtcaacca ttaaaccgat tcgcggtctt gatggcctgt aatttttaaa
     7981 aaaatccgac tttagttaca acatgctagt tattgatgtt ccattgtgct ccggcaacga
     8041 cggagcagaa aaagcctgcc aggggaaaaa tcgcaactgt taatttttta tttccacggg
     8101 tagaatgctc gccgtttacc tgtttcgcgc cacttccggt gcccatcatc aagaaggtct
     8161 ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt acgaattctc ctgtggttgt
     8221 tgcccttgat tatcataatc gtgatgacgc gctgtccttt gtcgacaaga tcgacccacg
     8281 cgattgtcgt ctgaaggtcg gcaaagagat gtttacattg tttgggccac agtttgtgcg
     8341 cgaacttcaa cagcgtggtt ttgatatctt tcttgacctg aaattccacg atattcctaa
     8401 cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc gtgtggatgg tgaatgttca
     8461 tgcctctggt ggggcgcgta tgatgaccgc agcgcgtgag gcactggttc cgtttggcaa
     8521 agatgcaccg cttttgattg ctgtaacagt gttgaccagc atggaagcta gcgacctggc
     8581 cgatcttggc gtgacactgt cacctgcaga ttatgcagaa cgtctggcgg cactgacgca
     8641 aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt
     8701 attcggtcag gagttcaaac tggttacacc gggcattcgt ccgcagggga gtgatgctgg
     8761 tgaccagcgc cgcattatga cgccagaaca ggcgttggcg gctggtgttg attatatggt
     8821 gattggtcgc ccggtaacgc aatcggtaga tccagcgcaa acactgaaag cgatcaacgc
     8881 ctctttacag cggagtgcat gatgagtgat tccaacagcc gtctggtcta ctcaacggaa
     8941 accggacgta ttgatgagcc aaaagcggcc ccagtacgcc ctaaaggcga tggtgtggtg
     9001 cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg tttgcctgat taccggtatc
     9061 gatctcgatg atgccgaact gacaaaactt gcagcggaac tgaagaaaaa atgcggctgc
     9121 ggcggagcag ttaaagatgg agttattgaa atccagggcg ataagcgtga tttattaaaa
     9181 tcactacttg aagcgaaagg gatgaaggta aaactcgcag gcggttaaca taaaaagcca
     9241 cggatatatc cgtggctttc gaatatttta ctgtgtgtat tattcagtta tttcgtactg
     9301 aacaaaatca agcgtattat aattgcgagt cgataatcga catttattta cgattattta
     9361 ccgacctggt gaccaataac accaccgaca gctgcaccac ctaatgtacc caacgtactg
     9421 ccatcggtca gtactgcacc gcctaatgcc cctgcacccg cgccgattgc ggtgttgcgg
     9481 tcccgtttag accagttaga acaggcactc agagacattg ccaaagtaat tgccagaaca
     9541 gccgcggtca tttttttgct cgttacaaac ataatactct ctcctgaatt tatgattcac
     9601 ggaagtaagc tctctataac tataatagct aagaataagc ctggtgaaat tattccgtga
     9661 aatctgctcg cgcgcagggt tatatcacgc aggtgataat gacttcctgt tatatcgctg
     9721 ataataattt tatatcttaa gcgtgttaat aacaggtaaa tagtcttaat tatcaaccag
     9781 gaatcatctt agagcggatg atttgccaaa ctgcaaatca tccgtagaga agggaaatgg
     9841 ttaaatgtca acgacgtgaa cggtgagttt actgtgctca agatgcatca gatcgctggc
     9901 gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca acgggaccga tggagtatga
     9961 atgtacagca ccaaatttcg agctgtcagt caggactatc acttcgcact ctttttccag
    10021 cacggcattg accacatcgg tacgcatcat atcgcgaccg gtgaatccag tttcaggttg
    10081 ccagccatca ataccaataa atgctttgct gaaatgcacc tgctggatgc actggcgtgt
    10141 caacgggcca accatacttt cgcttttttt ctggtacacg ccaccgagca aaatgacttc
    10201 acaaggcgcg tctttcagca aatgcgcgat gtagctgctg accgtgatga tagtgacatt
    10261 tttcttctgc tcccccagag tccgggcgag cagggcattg ctgctgccat tttcgataaa
    10321 gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg gcgagttcac gcttcagcgt
    10381 atagttgctc atcatacggg tttcgacgtc atcactatca agcgaaactg caaagccatg
    10441 tgcacggcgg aggtaactca gtttttcgag ggtgttgaga tcctggcgaa tggtaacttc
    10501 agaaactcca gtggcttttg ccagatcggt tacgctaacc tgaccctggt caatgaccat
    10561 ctgtagaata gtttgttgtc gggaattcat cgctgtaatt cttataacgt tataatacta
    10621 aataaaaaaa tactgacgcg ccgggcatcc tcaaatagga aaacctgaag gcacgattgt
    10681 taaatctccc acggtgcctt cgggatgtct ggcgtagatt cagcaacctg gcgcaagagt
    10741 tctgctttaa gtatctcgag attatggatc gcagagtggt aatcgcctgc cacaaggata
    10801 tctaacacaa tatcaatacg ttcggcaacg cgctgggcat caaattcaga catcaaggac
    10861 atccttatag caaaagtgga aaatgaataa tgcaaaaatt gccgctaaaa gagaagtgtt
    10921 taacagcaac ggctaattat catccaggaa tacgatatat aatgacggga tatagcgcta
    10981 agtatatata ttcatctact tatgcgcgct tcagatagcg tttataccag cgttcgaagg
    11041 cgacggcggg catcggtttg gcaaacaaaa atccttgccg ctcattgatc ccgttcttgg
    11101 ttaaaaaagc atcttccttt gcactctcta caccttcggc gatcacctga agattcaatg
    11161 cctgggccac agcgacgatc gcccggacca gtgactgcga gacaggttgt ttgtgaatat
    11221 ctcgaacaaa aacctggtca agtttgatgg catcgatcgg aaagcgcgcc agttgcgaaa
    11281 gtgaagagta gccggtacca aaatcatcca gatgcacttg cgcacctagt tggctaaatt
    11341 gttgaataac agacagtgcc agttcatcat tctcaatcag acaactctct gtcagttcaa
    11401 catctatagg gcagtattca aaattgagtt cctggagaac ctgtttcagg gcggtgaaaa
    11461 tggtttgatc ggcgagctga cgtgcagaaa tatttaccgc cactcgcagg tttatgcctt
    11521 tatcccgcca ctttgccacc tggcgtacga catcgagaat cacccaacgg cctaaaggca
    11581 caattagccc tgactcttcg gcgtaggaaa tgaagtccaa cggtggaatc aacccacgtt
    11641 caggtgactg ccaacgtact agtgcttcca gactgcgcac ttcgccacgc caggtgattt
    11701 tcggttgata gtgaataacc aactgatcgt tttccagtgc tttacgcaag ttggtatcca
    11761 gccagagata ttcaaatacc cgttgattca tttctggggt aaaaacgcaa aattgtcctc
    11821 gtccgccttc cttcgctgtg tacattgctg tgtcggcgtg acgaataata gccgtgctgt
    11881 ctgaaccatg ttcgggagag agtgcaatac ctactgaaca gctggtataa acttcaatta
    11941 aaccaatgcg aaaggggagc cgtaagcggg tcaaaattcg tgatgccatt gcttccagcg
    12001 cgctttgtga ggtgttggat gccagtacca gaaactcatc cccacctgga cgcgccaaca
    12061 cctggtcatg ttcgagacag cttaaaatag ccaatgacac gtcgcgtaat aactggtcac
    12121 caaacaaatg cccataggcg tcgttgacct ttttgaaatt atccaaatca agatacacaa
    12181 ccccaacttt attgttatct gcatgattaa tagcgtgatc gattaaatcc tgcattgcgt
    12241 tacgattcgg cagtccggtg atactgtcgg tatttgccag aatacgcagt cgctcctgag
    12301 cgcggcgctc ttcggtaatg tcggtgccgg aacagattaa aaaaatctcg cttttgccac
    12361 tgccgctgtg gacaaattta ttgcgaaaca gaaacagccg ctggccttta catgttggta
    12421 tccacagttc gacttcatat gcattgccgc ttcgaaaaaa tacacggtta ttgcgcctgg
    12481 atgccgcagc ttcacgacgg ctcataaaca gtttaaacac gctttggcca atgacgtcgt
    12541 gttctttcaa cccagtgtaa tcttcacata accgattgaa gcgttggata ttcccccggc
    12601 tgtcgagaat cactattgcg gaatgtgctt cagaaactac ctgctcagca aaggaaagcc
    12661 cgtgtgataa atcacgagca actgccggtg tgtcatgcca tgcagacgcg ttgccagccc
    12721 attcccgttt attgattttt cgtcctacca gatgcacaga aagatcgctc tcatcgacgg
    12781 tcagactcat catcaggctg gaggtgatga ccgtcatttc cctgatgc
//
LOCUS       DAJVTY010000121        12793 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVTY010000121 DAJVTY010000000
VERSION     DAJVTY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.121"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1330
                     /locus_tag="OQQ66_002995"
     CDS             <1..1330
                     /locus_tag="OQQ66_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS1905842.1"
                     /translation="IATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQD
                     TRENFVQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQET
                     ERRRRERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARI
                     AAFEAERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIA
                     NQTKSIAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQ
                     DAETKAVELTVRAKAEKEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDE
                     QTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNL
                     AEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1569..1628
                     /locus_tag="OQQ66_002996"
     CDS             1569..1628
                     /locus_tag="OQQ66_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1905843.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1945..2004
                     /gene="ibsE"
                     /locus_tag="OQQ66_002997"
     CDS             1945..2004
                     /gene="ibsE"
                     /locus_tag="OQQ66_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1905844.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2124..3557)
                     /gene="hldE"
                     /locus_tag="OQQ66_002998"
     CDS             complement(2124..3557)
                     /gene="hldE"
                     /locus_tag="OQQ66_002998"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1905845.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3605..6445)
                     /gene="glnE"
                     /locus_tag="OQQ66_002999"
     CDS             complement(3605..6445)
                     /gene="glnE"
                     /locus_tag="OQQ66_002999"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1905846.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6468..7769)
                     /gene="ygiF"
                     /locus_tag="OQQ66_003000"
     CDS             complement(6468..7769)
                     /gene="ygiF"
                     /locus_tag="OQQ66_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1905847.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8011..8631
                     /locus_tag="OQQ66_003001"
     CDS             8011..8631
                     /locus_tag="OQQ66_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1905848.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8695..9933
                     /gene="cca"
                     /locus_tag="OQQ66_003002"
     CDS             8695..9933
                     /gene="cca"
                     /locus_tag="OQQ66_003002"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1905849.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10090..10911)
                     /gene="bacA"
                     /locus_tag="OQQ66_003003"
     CDS             complement(10090..10911)
                     /gene="bacA"
                     /locus_tag="OQQ66_003003"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1905850.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11002..11370)
                     /gene="folB"
                     /locus_tag="OQQ66_003004"
     CDS             complement(11002..11370)
                     /gene="folB"
                     /locus_tag="OQQ66_003004"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1905851.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11475..12092
                     /gene="plsY"
                     /locus_tag="OQQ66_003005"
     CDS             11475..12092
                     /gene="plsY"
                     /locus_tag="OQQ66_003005"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1905852.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12105..>12793)
                     /gene="ttdR"
                     /locus_tag="OQQ66_003006"
                     /gene_synonym="dan"
     CDS             complement(12105..>12793)
                     /gene="ttdR"
                     /locus_tag="OQQ66_003006"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCS1905853.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIM
                     LRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
                     GKPDEGYQVM"
BASE COUNT         3092 a         3624 c         3205 g         2872 t
ORIGIN      
        1 catcgccact gcggcgcaga cgttggggca acgcacgcta tcaccagaag acttacgtat
       61 gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcacaaa tgaccatgca
      121 tgagttacag gatacccgcg agaacttcgt gcagggagta caaaatactg tcgcagaaga
      181 cctgtcgaaa aacggtcttg agctggaaag cgtttcactt accaacttta accagacctc
      241 gaaagaacat ttcaatccga acaatgcctt tgacgccgaa ggtttaacca aactgattca
      301 ggagacggag cgccgtcgtc gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt
      361 gcgtgagaaa aaccgtgatg cactttcgcg caagttggag attgaacaac aagaagcgtt
      421 tatgacgctt gagcaggagc agcaggttaa aacccgtact gccgaacaga atgcacgtat
      481 tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc tggctgaacg
      541 acaaattcag gaaacggaga tcgagcgcga gcaggccgtc cgctcaagaa aggttgaggc
      601 tgaacgtgaa gttcgtatta aagagatcga acagcagcag gtcaccgaaa tcgccaacca
      661 gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc
      721 taaccttgca ctcgcagaag cagtaagtgc gcaacaaaac gtagaaacca ctcgacagac
      781 cgcagaagcc gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg atgcagaaac
      841 caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag agatgcaggc
      901 ggcggctatc gttaagttag ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc
      961 acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca gccttaaatt
     1021 caaactggcc ttgttgcagg cgctacctgc ggtaatagaa aaatccgttg agccgatgaa
     1081 atctattgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggca gcgctgcggg
     1141 tgatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat tatcagccgc
     1201 tctctcttac cgcacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc
     1261 aggcggctca ctggcggcat tgacttcacc cttaacctca acaactcccg tcgccgaaaa
     1321 cgtagaataa attttcagct atgaacgtcc ctggcacaaa aagccgggga ttttttatat
     1381 ctgcgttccg ctaaaaactg caaatgatca ggtcgttgca acgttttgcg tgaccgctcg
     1441 gggaaggcaa aactgcctct gggaaagcgt tgcgcgaggt ccggcgctca tcaacaatcg
     1501 gggagtcagc aaagggctga aacgggaaag cccctcccga ggaaggggcc ttacagaagg
     1561 aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta agtttcgcag
     1621 cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc ctttacttta
     1681 ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctag cccaaaaagc
     1741 cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac gttgcagcgt
     1801 tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg cggggtccgg
     1861 cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg ggaaagcccc tcccgaggaa
     1921 ggggccttat agaaggaaag ggttatgatg aagttcgtca tcatactggt tgtgttgtta
     1981 ctgttaagtt tcccgactta ctaacaactg atcagagggg ggagaaatcc tcccttaccc
     2041 ttgttccttt actttaggtt gaaaaaacaa cagcgtcaat aggcctgcct gctacgaagc
     2101 gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt cttgatgatg
     2161 ttagtggtcg agcaaccgtc ttcgaagttg agcaccagca cttcgccgcc gttggcccag
     2221 acttctttac tcccggcgat ctcttctggt ttatagtcgc cgcccttcac cagtaaatct
     2281 ggcaggatcc cggcgatcaa gcgctgcggc gtgtcttctt caaacgacac cacccagtcg
     2341 accgcttcca gtgcgcccag cacaatcata cgctgttcga gcgggttaac ggggcgggaa
     2401 tcccctttca gccgtttggt ggaggcatcg ctgttcacag cgacaatcag acggtcaccc
     2461 agtttgcgag catttgccag ataggagacg tgcccggcat gcaggatgtc aaagacaccg
     2521 ttggtcatta ccaccttttc gccgcgttta cgcgctgccg ctacggccag cttcagttcc
     2581 tcttcggtca tcacgccaaa gccggtatct gcgcgcccac gcactgcatt ttccagctcg
     2641 atcggcgaaa ccgtggaggt tcccagtttg ccgaccacca cgccagccgc cgcattggca
     2701 aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag gacgccaatc
     2761 accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgggttgg catatgcagc
     2821 ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac taacagagcc
     2881 gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc ttcggtctta
     2941 catttaccga caacagcttc aaattccgag agattcggcg ttaacagcgt agcgccgcgg
     3001 tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacgcccgc tttacgcgcc
     3061 agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga aagcaccagc
     3121 gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg atcaacgcct
     3181 tcgaaacctt cttcgaaatc cagacggatc agctgttggt tgcgggaaag tacccgtaat
     3241 ttggtgatgg tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt gacgtcggcc
     3301 agagatttac tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac caggcgtgca
     3361 ttagcaccga gagaagcgat attcatcgcc acgttagccg cgccgcccgg acgttcttcg
     3421 atggtattca ctttaaccac gggcaccggc gcttccggcg agatacggct ggtgggaccg
     3481 taccagtaac gatccagcat tacatcacca accaccatca ctcctgcacg ttcaaactct
     3541 gacagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc gataatacca
     3601 tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagatca cgctctgcgg
     3661 tgaagcaatc ctccgacaca tggcccggca attcctgtaa tgccagatga tgaagttcat
     3721 cgcgcagcgt cgtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt
     3781 tttgcgccag cagctccaga atacgcacgt tgtctgacca gcgcgttaac ttcggttttt
     3841 catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta attccgcctt
     3901 catcggcttt gatatcaaag cgattacgat gtttattgcc gagatgagcg cgcattttct
     3961 cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc atcataatct
     4021 cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact acacgcgcac
     4081 gcaccagcgc ctgatgctcc cacgtccagg cctcgttttt ctgataatca gcaaatgctt
     4141 ctgcggatgt caccagcatt cccgccgccc cggacggacg cagtcgtgca tccacttcat
     4201 acaaaatgcc ggaagaggtt cgcgtattga acagatgcat aatgcgttgc gccagacgca
     4261 gataaaactg tcgcccgtcg atttcccgct cgccgtcagt catcgcatcc attgggcaat
     4321 catggaggaa gatcaggtca agatcggaac tgtagcctaa ctcccagccg cccagcttgc
     4381 cgtagccgac caccgcaaaa ccacggcctt cgcgatcgtt gaggtgattt ggcttaccgt
     4441 agcgggccac catttgaacc cacgcctgct gaacgacggc atctatcata gcctctgcca
     4501 gccaggttaa gtgatcgctc actttcatca ccggtagcgt accagcgata tccgctgcgg
     4561 cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt tgctcttcgt
     4621 catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca tcggtcgccg
     4681 tcggctggta aagagtgttt ggatcgagca attcatccag caataatgga taacgcgcca
     4741 gctggctggc aatcatcggc gatgcggcac acagagaaat caaatgttta agcgccgcag
     4801 ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccacc agcaaggcgg
     4861 taatgcgcga cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt agcagatgtg
     4921 gcatcagatg gtcgagcacc tgacgtcctc gcggcccgat agtacgctta tccagctctt
     4981 tgcggaaatc agcaatcagc gttaggacct gtttgcgatc atcctcgcta agatgcgcca
     5041 gcactggcgt agtgtcatct tcctgcaacg catcctgcca cagctcacgc cactgttccg
     5101 acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta aacacccggc
     5161 gcacattggt catatgtgcg gtcagcgccc cggtcagttg cggccagtca gcaaagtcca
     5221 tcgcccacgc cagccgcgca cggttaagct catcagaagg aagcgtttgg gtttgttcgt
     5281 cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatatgcc actcgcaatt
     5341 gttcagcatc gttttcagaa agcagatgca gcgcggcaat gacgctgagc gttggcagta
     5401 aagagcgcga ctgcagcgac ggttcgcgcc caccgcgaat gagctggaac acctgaacga
     5461 taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc aaaccacgtc
     5521 gacgcacttc acgggcaatc atccctttca tgttgcgcag cgactgaatc acgctgaaat
     5581 cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta gcatagacgc
     5641 cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag tcgcgcccct
     5701 gctcctggta ataatcctcc aacgcggcaa agctcagcac cagcgggcca ctttcgccaa
     5761 acggacgcag ccgcatatcc acgcgataga cgaagccatc ctgcgttggt tgatccaaca
     5821 ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt tcccggcgtc
     5881 cgccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatct gaggagaaat
     5941 tcagttcccc accgcccagc ttacccatgc ctaaaatcag cagcggttgc gcttcgccct
     6001 gcgcattgca cggcgttccc cactcgcggc agcaagcgtc atacagccag tcacgcgcgg
     6061 cgacaatcag cgtctccgcc agatggctga gctgctgcaa tatgctctct tcagtaacca
     6121 aagccagcgt ttgcgcccag gcgatgcgca ccataatgcg ccgccggaat agccgcagct
     6181 cgcgcattaa cccggcttcg tcactcacat tgctgagcgc ctcctgcaac catgccgcgt
     6241 aatgctgcca ttcatcagcc tgcggcgatt ggctttccag ttccgtcagc cactctggat
     6301 gcgcaatcac gctgtcctgc acaaaatcac tgaaagtaag tactgacttc gcctgtgcgc
     6361 taagtgattt ctcggctaaa ggctctggca gccgctcaac aacggtctgc cagtactgct
     6421 gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta acgttttccg
     6481 ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc aatttcaatg
     6541 cgttgcccgg tcgcaatagc gtgacgcagc ccctgccagt tctccagcca ggcttgcgcg
     6601 acgacaggat catagtaacc cgccagcaac agtattgagt caatatcacg cgtcagacgt
     6661 ggcaactggt cacggtagcg atcgcccaac ggctggcaga aaacgctttt cagttcagcg
     6721 gcatggcggg aaagatggat atcggcaaag cgtttgaagg aatcgctcat tttactctgc
     6781 gctttggcat ctaaaaacgg ctgccaggct ttgcttacca gccattcggt caacgccagc
     6841 ttcgccattg ccgttttggt agagtagacc gccgtcacgg cagaaaccgc agaagcgatg
     6901 gtcgcttcgc attgagtcag caaatcgcgt aagtgagtgc tcgctttgcg cggcacaata
     6961 ccaccgaaca gcatcagtgt atgacggacc agaccaatgg ctgccagcac ctgttctttc
     7021 gccgcatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc taacgccagc
     7081 tcgagcgccg cttccagccc ctgttccaca tcggcttttg ccgcaacatg caaaatggtg
     7141 gtcggtttga tttcacgcgc cggattgccc tgcgccagat gatagccacg cgccgctttg
     7201 ctcaggctgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag tttcagcacc
     7261 gcgcgcgtgt cgccgctaag cagttccagt tccagctcac agataggttc agcaaattcc
     7321 cccgctttca cctccccctg atcgagagcg atttcaattc ggctgtcatc gaccgccacc
     7381 agccattttt cgcgataaaa atcggtgctg aacagcggtt gtacgcggga agcgagatcg
     7441 gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc gagcgtcggt
     7501 tcgctcaacg ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca
     7561 actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc catatcgtgc
     7621 ccacgcagcc agttatccgg cgtttcgtag taaatattca gcaactgcac ggggtcatgg
     7681 tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcact gtgattaaca
     7741 ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg tcacataagt
     7801 gacgatgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa
     7861 ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca agacctgaca
     7921 cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca ctactatcgt
     7981 tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat cggattaact
     8041 ttactcgcac ttagcgccac tgccgtctca cacgctgaag aaacgcgcta tgtttctgat
     8101 gaactgaata cctgggtccg tagcggtccg ggagatcatt atcgcctcgt gggcacggtt
     8161 aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta tgcccaggtg
     8221 aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac tgagccaagc
     8281 ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga taaactcacc
     8341 aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagc
     8401 gacagcgtga tcaacggatt aaaagaagaa aatcagaaat tgaaaaacga gctgattgtc
     8461 gcgcagaaaa aggtcgatgc cgccagcgta cagctggatg acaaacagcg caccatcatc
     8521 atgcaatggt ttatgtatgg cggtggcgtg ctggggcttg gcttgctgct cggtctggta
     8581 ctgccacacc tgatcccaac gcgcaaacgc aaagagcgct ggatgaacta aatcgccttc
     8641 ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga tggcgtgaag
     8701 atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt caaagacaga
     8761 gattgggtgg tggtcggcag tacgccacag gagatgctcg acgcgggcta ccagcaggta
     8821 ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc gctggcacgt
     8881 accgaacgga aatccggttc cggttacacc ggttttactt gctatgccgc accggatgtc
     8941 acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc ccaggacgat
     9001 aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc
     9061 catgtttccc ccgcttttgg cgaagatccg ttacgcgttc tgcgcgtggc gcgttttgct
     9121 gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct gatgcgcgag
     9181 atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa agagacggaa
     9241 agcgccctca ccacccgcaa tccccaggtg ttcttccagg tactgcgcga ttgcggtgca
     9301 cttcgcgttt tattcccgga aattgacgcc ctgtttggcg ttccggcccc tgccaggtgg
     9361 catccggaaa tcgacaccgg tattcatacc ttaatgacgc tctcaatggc ggcgatgctg
     9421 agtccgcagg ttgatgtccg tttcgcaact ttatgccacg atctcggtaa aggactgacg
     9481 ccgccagaac tttggccgcg tcatcatggg catggcccgg cgggtgttaa gttagtggaa
     9541 caactatgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag actggtggct
     9601 gagtttcacg atctcatcca caccttccca atgctgaacc caaaaaccat cgtcaaatta
     9661 tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc
     9721 gaggctgacg tacgcggcag aaccggtttt gaatcagcgg actacccgca aggccgttgg
     9781 ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagcggtcgt tgaagcggga
     9841 tttaaaggtg tggagattcg cgaggagctg acccgacggc ggattgcggc ggtagccagt
     9901 tggaaggaac aacgttgccc aaagcctgac tgagtataaa actaaacgtt aacacgaacc
     9961 atcccctcgc cccttcgggg agagggtgag gggaacagac cagcaccggt gcgaacaatc
    10021 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    10081 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    10141 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    10201 caccacaaaa gcggtgataa acccaacggc aaacatcggg atatcgccgg ttgtcaggaa
    10261 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    10321 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    10381 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    10441 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    10501 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    10561 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    10621 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    10681 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    10741 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    10801 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    10861 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    10921 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    10981 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    11041 tacaccaaca ttcgccgccc gcgccactgc gcctggcttg ctgagtttga tgcgcaccca
    11101 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    11161 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    11221 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    11281 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    11341 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    11401 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    11461 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    11521 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    11581 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    11641 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    11701 taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    11761 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    11821 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    11881 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    11941 ggtggtttaa gccacaattc accttcccag tttcgatgct ctcttgcctg atcctgctgc
    12001 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    12061 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    12121 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    12181 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    12241 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    12301 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    12361 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    12421 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    12481 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    12541 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    12601 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    12661 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    12721 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttgcgtgacg
    12781 tcatcgacca gtc
//
LOCUS       DAJVTY010000122        12739 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVTY010000122 DAJVTY010000000
VERSION     DAJVTY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.122"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OQQ66_003007"
     CDS             complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OQQ66_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS1905854.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1197..2282
                     /gene="dmlA"
                     /locus_tag="OQQ66_003008"
     CDS             1197..2282
                     /gene="dmlA"
                     /locus_tag="OQQ66_003008"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS1905855.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2532..4142
                     /gene="yeaV"
                     /locus_tag="OQQ66_003009"
     CDS             2532..4142
                     /gene="yeaV"
                     /locus_tag="OQQ66_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS1905856.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4174..5298
                     /gene="yeaW"
                     /locus_tag="OQQ66_003010"
     CDS             4174..5298
                     /gene="yeaW"
                     /locus_tag="OQQ66_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS1905857.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5354..6319
                     /gene="yeaX"
                     /locus_tag="OQQ66_003011"
     CDS             5354..6319
                     /gene="yeaX"
                     /locus_tag="OQQ66_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1905858.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OQQ66_003012"
     CDS             complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OQQ66_003012"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1905859.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OQQ66_003013"
     CDS             complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OQQ66_003013"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1905860.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OQQ66_003014"
     CDS             complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OQQ66_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1905861.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OQQ66_003015"
     CDS             complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OQQ66_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1905862.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10833..>12739)
                     /locus_tag="OQQ66_003016"
     CDS             complement(10833..>12739)
                     /locus_tag="OQQ66_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1905863.1"
                     /translation="DDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVEAG
                     TGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKGRS
                     NYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPLVT
                     STNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPEAD
                     VMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLAQS
                     AQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDAAF
                     ERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSWIF
                     TSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSARQL
                     AAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFVSA
                     GNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFDEV
                     QLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIARA
                     VRFLAIPSSR"
BASE COUNT         3023 a         3424 c         3177 g         3115 t
ORIGIN      
        1 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
       61 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
      121 ggagaaaatc aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc
      181 tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
      241 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
      301 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
      361 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
      421 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
      481 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
      541 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
      601 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
      661 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
      721 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
      781 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
      841 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
      901 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
      961 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
     1021 acggcggcaa aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc
     1081 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
     1141 cgtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
     1201 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggtaaagaa gtccttcctg
     1261 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
     1321 aaatggggtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
     1381 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
     1441 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
     1501 agtacatcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
     1561 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
     1621 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
     1681 ttacacgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
     1741 cacgtaagac gctaacttat gccactaaat cgaacggttt agccatcagc atgccgtact
     1801 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
     1861 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
     1921 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
     1981 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
     2041 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacgattt
     2101 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
     2161 ataacggtat tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata
     2221 tgaaaggcaa tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
     2281 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttttattc gttctttgca
     2341 gtaaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
     2401 cattcttaat tgtttaattt atgtaatatt caaataatca atttgttaaa cacaacatac
     2461 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca
     2521 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
     2581 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
     2641 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg
     2701 ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc
     2761 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
     2821 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcaaacac
     2881 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtt ccctactctt
     2941 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
     3001 atcactttca tgtgcggaaa aacaaaggtc tgagcctttc cggcattatt gctgctatta
     3061 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
     3121 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
     3181 ccgcactgac aggtttaccc gacaacttca ccgtgcaggc atttgtgatc ctgctttctg
     3241 gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca
     3301 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg
     3361 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
     3421 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
     3481 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
     3541 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
     3601 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
     3661 acgtcccaca ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt
     3721 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
     3781 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
     3841 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
     3901 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
     3961 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
     4021 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
     4081 ttgaagccct ggaaaaaaca ccagtgatcc ctgcgggaac cgtattcaaa ggcgacaact
     4141 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
     4201 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
     4261 accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
     4321 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
     4381 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
     4441 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
     4501 ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
     4561 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
     4621 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
     4681 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
     4741 cgctttacca cccgcacgtc tgccaactgg aagaacattg tcgataacta tctcgagtgc
     4801 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
     4861 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
     4921 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
     4981 atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
     5041 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
     5101 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
     5161 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
     5221 gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
     5281 caggtgttta aggactaatg acatcggcgg cggtattttc cgctgctggg ctgatttttg
     5341 atggagtaca gcaatgtcag actatcaaat gtttgaagta caggtgagcc aggttgaacc
     5401 ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc
     5461 gttcaccgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc agtacagcaa
     5521 tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct
     5581 ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcaatcg
     5641 gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
     5701 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca
     5761 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
     5821 tgatgagcta gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg
     5881 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
     5941 ctgtggtccc gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
     6001 cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac
     6061 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
     6121 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
     6181 aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
     6241 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
     6301 cctggtgttg gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg
     6361 cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
     6421 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
     6481 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg
     6541 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg
     6601 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
     6661 ttccaccagc gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
     6721 gctgtccagt tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc
     6781 acgcacgaca aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc
     6841 taacagttgc agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg
     6901 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc
     6961 cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
     7021 taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc
     7081 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
     7141 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat
     7201 caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
     7261 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
     7321 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
     7381 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
     7441 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
     7501 attgttttct ctttagtggg cgtcaaaaaa acgccggatt aaccggcgtc tgacgactga
     7561 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     7621 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     7681 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
     7741 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     7801 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     7861 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     7921 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
     7981 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
     8041 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
     8101 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
     8161 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
     8221 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
     8281 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     8341 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
     8401 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
     8461 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
     8521 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     8581 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
     8641 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
     8701 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
     8761 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     8821 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
     8881 accgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     8941 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     9001 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccac cccttgttgc
     9061 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc
     9121 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     9181 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     9241 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     9301 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
     9361 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     9421 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
     9481 actctcgcgg gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga
     9541 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     9601 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg
     9661 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
     9721 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
     9781 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
     9841 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     9901 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgcggccttt aatggcgtcc
     9961 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    10021 acattctttt gaaccgccat gatgcgactc ctttttttct cagagcatac tcttaagatt
    10081 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    10141 tgttccaccg ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct
    10201 tccgtcgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga
    10261 tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt
    10321 cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg
    10381 ttttcatcac gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc
    10441 agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag
    10501 acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg
    10561 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg
    10621 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    10681 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    10741 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    10801 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    10861 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    10921 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    10981 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    11041 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc
    11101 gggtcatccg gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca
    11161 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg
    11221 acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc
    11281 atggtagcgc ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata
    11341 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    11401 gaacctggct ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg
    11461 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    11521 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    11581 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    11641 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    11701 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    11761 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    11821 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    11881 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    11941 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    12001 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    12061 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    12121 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    12181 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    12241 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    12301 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    12361 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    12421 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    12481 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    12541 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    12601 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    12661 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    12721 tctggtgcaa aatcgtccg
//
LOCUS       DAJVTY010000123        12738 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVTY010000123 DAJVTY010000000
VERSION     DAJVTY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.123"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..471
                     /locus_tag="OQQ66_003017"
     CDS             <1..471
                     /locus_tag="OQQ66_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCS1905864.1"
                     /translation="YQERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEP
                     DVPEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLP
                     PGISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(480..878)
                     /gene="vapC"
                     /locus_tag="OQQ66_003018"
     CDS             complement(480..878)
                     /gene="vapC"
                     /locus_tag="OQQ66_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1905865.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTPMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(878..1105)
                     /gene="vapB"
                     /locus_tag="OQQ66_003019"
     CDS             complement(878..1105)
                     /gene="vapB"
                     /locus_tag="OQQ66_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1905866.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1187..1606
                     /locus_tag="OQQ66_003020"
     CDS             1187..1606
                     /locus_tag="OQQ66_003020"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1905867.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1593..6464
                     /gene="traI"
                     /locus_tag="OQQ66_003021"
     CDS             1593..6464
                     /gene="traI"
                     /locus_tag="OQQ66_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCS1905868.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6484..7230
                     /gene="traX"
                     /locus_tag="OQQ66_003022"
     CDS             6484..7230
                     /gene="traX"
                     /locus_tag="OQQ66_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1905869.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7285..7845
                     /locus_tag="OQQ66_003023"
     CDS             7285..7845
                     /locus_tag="OQQ66_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1905870.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7976..8188
                     /locus_tag="OQQ66_003024"
     CDS             7976..8188
                     /locus_tag="OQQ66_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1905871.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8432..8893
                     /locus_tag="OQQ66_003025"
     CDS             8432..8893
                     /locus_tag="OQQ66_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1905872.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8939..9148
                     /gene="hha"
                     /locus_tag="OQQ66_003026"
     CDS             8939..9148
                     /gene="hha"
                     /locus_tag="OQQ66_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1905873.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9187..9777
                     /locus_tag="OQQ66_003027"
     CDS             9187..9777
                     /locus_tag="OQQ66_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1905874.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10017..10277
                     /gene="repA"
                     /locus_tag="OQQ66_003028"
     CDS             10017..10277
                     /gene="repA"
                     /locus_tag="OQQ66_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1905875.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10501..10575
                     /gene="tap"
                     /locus_tag="OQQ66_003029"
     CDS             10501..10575
                     /gene="tap"
                     /locus_tag="OQQ66_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1905876.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10568..11425
                     /gene="repA"
                     /locus_tag="OQQ66_003030"
     CDS             10568..11425
                     /gene="repA"
                     /locus_tag="OQQ66_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1905877.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12477..>12738)
                     /locus_tag="OQQ66_003031"
     CDS             complement(12477..>12738)
                     /locus_tag="OQQ66_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1905878.1"
                     /translation="QAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCG
                     LRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3088 a         3212 c         3779 g         2659 t
ORIGIN      
        1 tatcaggaac gaccgaaggt cgctccggag tttattccgc gtgacatcaa cccggaaatg
       61 gagaaccgtc tgagtgccgt acttgccgca agggaagcag aaggtcgtca gatggccagc
      121 ctcttcgaac cggatgtccc ggaggttgtt tccggagaag acgtgactca ggctgaacag
      181 ccgcaacagc cggtgtctcc tgtcatcaac gataagaagt cagattcagg tgtgaatgtt
      241 ccggcagggg ggatcgagca ggagctgaaa atgaaaccgg aagaagagat ggaacagcaa
      301 ctgccacccg ggatcagtga atccggtgaa gtggtggata tggccgctta tgaggcatgg
      361 cagcaggaaa atcatccgga cacctggcag cagatgcagc gtcgtgaaga ggtgaacatc
      421 aatgtgcacc gggaacgcgg ggaggatgtt gagccgggag atgatttctg atgaacaggt
      481 cagctccagt cttcagttct caggccgccc acacgttcaa attcccgggt gttattagtc
      541 acaataatca gcccccgact gcgggcatga cctgcgatca tttgatcaaa tggcccgaca
      601 gggcgtccct gacgggcaag ttctgctctt atctggccgg tgtgtgtggc tgcagcagcg
      661 tcgtaatcca gaacatcaat gcgggaaaca aatccctcga tcacagcgag attacgttca
      721 ggcatctggc ttttttctgc accatatatc agctccatcg gggtgaccga actgatacac
      781 attctcccct ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt ctttatcgta
      841 aaaatgcaga tgttggtatc gagcataaac ttcagcatca gaatgactcc ctttcctgca
      901 tgccgggctg ttccctgtta tccataaaat cggcgctgac gctgtgtccg tcgaaccatt
      961 cgtcccatgt ctctccggct ggcgtaatga ttctggttcg tccgacagca atcacttcaa
     1021 cgcgttttac gttttccggc aatgcaaccg cttttggcag tctgaccgcc tggctgcggt
     1081 tgctgagaaa tacggtggtt tccattattt gcctccttat gtggatatgt cttgtgtata
     1141 tccattgtgg gattgatgcc gggatatgtc aaagggatat acgtttatga tgagtatcgc
     1201 gcaggtcaga tcggccggaa gtgccggtaa ttattatacc aacaaggata attactatgt
     1261 gctgggcagc atgggagaac gctgggccgg ccagggggct gaacaactgg ggctgcaggg
     1321 cagtgtcgat aaggatgtct ttacccgcct tctggagggc aggctgccgg acggggcgga
     1381 tttaagccgc atgcaggatg gcagtaacag gcatcgtccc ggctacgacc tgaccttctc
     1441 cgctcccaaa agtgtctcca tgatggccat gctcgggggg gacaaacgtc ttattgaagc
     1501 acataaccag gccgtggatt ttgctgtccg tcatcacccg tgtggaggca ctggcctcca
     1561 cacggatgat gacggacgga cagtcagaaa cggtgctgac cggtaatctg gtgatggcac
     1621 tgtttaacca cgacaccagt cgcgatcagg aaccacagtt acacacgcat gcggtggtga
     1681 ctaatgttac gcagcataac ggcgaatgga agacactgag cagtgacaaa gtggggaaaa
     1741 cggggttcat tgagaatgtg tacgctaatc agattgcctt tggcaggctc taccgggaaa
     1801 aactgaaaga gcaggttgag gcgctgggct atgaaactga agtggtcggt aagcacggta
     1861 tgtgggaaat gccgggtgta ccggtggagg ccttttccgg acgctcacag actatccggg
     1921 aggccgtcgg ggaagacgcc tcgctgaaat cccgggatgt ggcggcgctg gatacgcgta
     1981 aatccaaaca gcacgtcgat cctgaagtca gaatggccga gtggatgcag acgctgaagg
     2041 aaaccgggtt cgacatccgg gcatatcgtg acgcagcgga acaacgggca tatacccgca
     2101 cgcagacacc cggacccgct tcacaggacg ggccggatgt gcagcaggcg gtgacacagg
     2161 ccattgccgg attaagtgaa cgcaaagtgc agtttatgta tacggacctg ctggccagga
     2221 cggtcggcat actgccgccg gaaaatggtg tgattgaacg ggcacgcgcc ggtatcgatg
     2281 aggccatcag ccgtgagcag cttatccccc tcgatcgtga gaaggggctg ttcacgtccg
     2341 ggattcatat gctcgatgag ctgtcagtcc gggcactcag tcgtgacatc atgaaacaga
     2401 accgggtgac cgtacatccg gagaaaagtg tcccccggac ggccggttac agctatgccg
     2461 tcagtgttct ggcacaggac cgtccgtcgc tggccattgt gtccgggcag ggcggtgcag
     2521 ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc ccgggagcag gggcgggagg
     2581 tgcagattat cgctgctgac cgtcgctcgc agatgaacct gaagcaggat gaacggctgt
     2641 ccggtgaact gataaccgga cgtcgtcagt tgctggaagg catggccttc acgccgggca
     2701 gtaccgttat cgtcgaccag ggcgaaaaac tctccctgaa agagacgtta accctgctgg
     2761 atggtgccgc acgtcataac gtacaggtcc tgataactga cagcgggcag cgaaccggta
     2821 caggcagtgc actgatggcc atgaaggatg ccggggtgaa cacatatcgc tggcaggggg
     2881 gagaacagcg accggccacc atcatcagtg aaccggaccg ttatgtccgc tatgcccggc
     2941 tggccggaga ttttgcggcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg
     3001 gagtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acacagggcg
     3061 tgctcggaca cccggaggta accatgaccg ccctgtcacc ggtctggctg gacagccgga
     3121 gccgttatct gcgggatatg taccgcccgg gaatggtgat ggagcagtgg aacccggaga
     3181 cacgcagtca tgaccgctat gtgacagaac gtgtgacggc gcagagtcac agcctgaccc
     3241 tgcggaatgc gcagggcgaa acgcaggtgg tgcgtatttc ttccctggac agcagctggt
     3301 cgctgttccg gccggaaaaa atgccggtgg cagacggtga gcgtctgagg gtgacaggga
     3361 aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
     3421 atgcgatgac ggttgttgtg ccgggacggg ctgaaccggc caccctgcct gtgagcgatt
     3481 caccgttcac ggcactgaag ctggagaacg gctgggtgga aacgcccggg cattccgtca
     3541 gcgacagtgc gacggttttt gcctccgtca cacagatggc aatggacaat gccaccctga
     3601 acggtctggc ccgcagtggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
     3661 ctgcggaaaa acttgcccgc catccttcct ttacggtggt ttctgagcag ataaaggcgc
     3721 gtgccggtga gacattgctg gaaaccgcta tcagtctgca gaaagccggg cttcacacgc
     3781 cggcacagca ggccattcat ctggcccttc ctgtgctgga aagtaaaaac ctggccttca
     3841 gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc ggttttgctg
     3901 acctgggagg ggaaatcaat gcgcagataa aacggggtga tttactgtat gtggatgtgg
     3961 caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca
     4021 ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc gctgatggag agagtacctg
     4081 gcgaactcat ggagaaactg acatcaggac agcgcgccgc cgcccgtatg atactggaaa
     4141 cgtccgaccg tttcacggtg gtgcagggct atgccggtgt gggtaagacc acacagttcc
     4201 gggcggtgat gtcagccgtg aacatgctgc cggagagtga acgtccccgt gtcgtggggc
     4261 tggggcccac acaccgtgcg gtcggggaga tgcgcagcgc cggcgtggat gcacagacac
     4321 tggcgtcctt tctgcatgac acgcagctgc agcagcgcag cggagaaacg ccggatttca
     4381 gcaacacgct gttccttctc gatgagagct ctatggtggg caataccgac atggcacggg
     4441 catacgccct gattgcggcc ggtggcggtc gggctgtggc cagcggtgac acggaccagc
     4501 tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt gctgccgatg
     4561 tggtcatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc
     4621 tgattaaccg ggatgtggaa agggcgctgt ccgggcttga gcgtgtgaaa ccgtctcagg
     4681 tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc agtcacagcc
     4741 aggaagcgaa actggcagaa gcgcagcaga aggcgatgct gaaaggcgag gcttttccgg
     4801 atgtccccat gacactgtat gcagccattg tccgcgacta taccggcagg acaccggaag
     4861 cacgggagca gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca
     4921 tgattcatga tgcacgggaa aaggccggag aactggggca ggtgcaggtc atggtgcctg
     4981 tcctgaacac agcgaatata cgcgacgggg agctgcgtcg tctctccacc tgggagaata
     5041 acccggatgc ccttgccctg gtggataatg tgtatcaccg gattgccggt atcagcaagg
     5101 atgacgggct gataaccctg caggatgcgg aaggtaacac gcggctgatt tcgccccggg
     5161 aggcggtggc tgaaggcgtc acgctgtaca ccccggacac catccgggtg gggaccggtg
     5221 acaggatacg cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga
     5281 cggtgacagc ggtttccggt gacagtgtca cactttctga cggtcagcag acccgtgtga
     5341 ttcgccccgg tcaggagcgg gcagagcaac atattgacct ggcctatgcc atcaccgccc
     5401 acggtgcaca gggggcgagt gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc
     5461 ggaaactgat ggccggcttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg
     5521 tgcaggtgta caccgataac cgtcagggct ggacggatgc catcaacaat gccgtacaga
     5581 aaggaactgc ccacgatgtg tttgagccga aaccggaccg ggaggtcatg aatgcagagc
     5641 ggctgttcag tacggcgcgg gagctgcggg acgtggcggc aggccgtgcc gttctccgtc
     5701 aggcggggct ggccggggga gacagtcctg cacggtttat tgctccggga cgtaaatatc
     5761 cgcaaccgta tgtggcactg ccggcgtttg accgtaacgg caagtccgcc ggtatctggc
     5821 tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt gaaggtcggg
     5881 taaaaggcag cggggatgcg cagttcgtgg ctctgcaggg cagccgtaac ggagaaagcc
     5941 tgctggctga taatatgcag gatggcgtcc ggattgcccg tgataatcct gacagtggtg
     6001 tggtggtaag aatcgccggt gaaggccgtc cgtggaatcc cggtgccatc accggtggcc
     6061 gcgtgtgggg ggatatcccg gacaacagcg tccagccggg agccggaaat ggcgaaccgg
     6121 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
     6181 gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
     6241 aaacagagca ggctgtcagg gaaattgccg ggcaggagcg tgaccgggct gccataactg
     6301 aacgggaagc cgcgctgccg gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg
     6361 tccgggaagt tgcccgggaa aatttgctgc aggagcgtct gcagcagatg gagcgggata
     6421 tggttcgtga cctgcagaaa gagaaaaccc cgggcggaga ctgatacagg aagacaaaca
     6481 ctgatgacaa cggataacac gaacacgaca cgtaacgatt cactggctgc acggaccgat
     6541 acctggttgc agtcatttct ggtctggtca cccggacagc gggatatcat caaaacggtg
     6601 gcactggtgc tgatggtact ggaccacatc aatctgatat tccagctgaa gcaggaatgg
     6661 atgtttctgg ccgggcgagg agcctttccg ctttttgccc tggtgtgggg gctgaatctg
     6721 tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg gggaattatt
     6781 gcccagttcg catattatct ggcaggcttt ccctggtatg aggggaatat cctgtttgcc
     6841 tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca
     6901 gcggccatac tcctgatggc cctgtggggg cctttgtccg gcaccagtta cggtattgcc
     6961 gggttgctga tgctggcagt cagcaaccgg ctgtaccggg cggaagacag agcggaacgt
     7021 ctggcgctgg tcgcctgcct gctggctgtt attccggcgc ttaaccttgc caccagtgat
     7081 gcagcggcgg tggccggtct tgtgatgacg gtgctgaccg ttggtctggt gttgtgtgcc
     7141 ggaaaatcat taccccgttt ctggcccgga gactttttcc cgacgttcta tgcctgtcat
     7201 ctggctgtgc tgggcgttct ggcgctgtga cgggtgtggt atctttggcc gcaagaggat
     7261 gattcgtcag aggcagaaca cagcatgaca gagcagaagc gaccggtact gacactgaag
     7321 cggaagacag agggaacagc gactgtccgc agccggaaaa ccatcatcaa tgtcaccacg
     7381 ccaccaaagt ggaaggtgaa aaagcagaag ctggccgaga aagcggcccg tgaagcagag
     7441 ctggcggcaa aaaaagcgca ggccagacag gcgctgtcca tttatctgaa cctgcccacg
     7501 ctggatgagg ctgtgaatac cctgaagccc tggtggccgg gattatttga cggtgacacg
     7561 ccccggcttc tggcctgcgg tatccgggac gtgttactgg aagacgtggc gcagcggaat
     7621 atcccgctct cgcataaaaa actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc
     7681 tatctgtgtg ccatgaaagc cggtgcctgc cggtatgaca cggaagggta tgtgacggag
     7741 catatttctc aggaggagga agcgtatgcg ggagcgcgtc tggcgaaaat tcgtcgccag
     7801 aaccggataa aggcagaact tcaggccgtg cttgatgaga aataaaaaaa gcctccccca
     7861 gagagaggag gcagggaaat aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc
     7921 cagtccactg aattatttat cacaataata ctgacaacta aagggggaca agaaaatgaa
     7981 tggattcaga aacagttcac ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg
     8041 ggccgttatt ctggaagtca gtggacgctg gatggaagcg gcagaagcat ggcgacgggc
     8101 tgcctgtgtt gcgcctagga cagactggca gcagtttgcc cggaaaaggg ctgaacactg
     8161 ccaccggcgc tgccggggaa gggggtaacg aaaaaagcag ccttcattac tccaggagtc
     8221 agaaggctga aaggagttta tgagatttgc ttcttaatat ccggtcgggc agtatgaccg
     8281 gacagttctg agtagcacag agtcttttcc gcattaagtc gattttttat attttgtgat
     8341 taatttcaca aaataaggtg ttattcaatg tgtgctgcac tattcagggg ggctgaaata
     8401 caggtgccat ttatctgata tggagaataa catgaggaaa tatattccac tggtattatt
     8461 tatcttttca tggccggtat tgtctgcaga tattcatgga cgggttgttc gtgtactgga
     8521 cggtgatacc atcgaggtca tggactcact gaaggcggtg cggatcaggc tggtaaatat
     8581 tgatgcaccg gaaaagaaac aggattatgg ccggtggtca acagacatga tgaaatcact
     8641 ggttgcagga aaaacggtta ccgtcacata ttttcagcga gatcgttatg gtcgtattct
     8701 cgggcaggtt tatgcaccag acggaatgaa tattaatcag tttatggtcc gggccggggc
     8761 tgcctgggta tatgagcagt acaacacaga tcccgtgcta cctgtccttc agaacgaagc
     8821 cagacaacaa aaacgtgggc tctggtcaga tgctgatccg gttcccccct ggatatggag
     8881 gcaccgtaaa taaagcgatg gcaatcctat cattcatctg ttctcagcgg gaataaaaat
     8941 ggcaaaaaca aaacaggaat ggctgtatca gttacggcga tgttcatcag tcaatacgct
     9001 ggaaaaaatt attcataaaa acagagacag tttatcaaac agtgaacgtg aatcatttaa
     9061 ttcagcagca gaccaccggc tagcggaatt aatcaccgga aagttatatg acaggatacc
     9121 aaaagagata tggaaatatg taaggtaata attcagcaat aattaattgc caggagcaga
     9181 taaataatga agttgattat ttttattctc attgttctga ttattgcagc gctacttatc
     9241 cggattatcc tcagaagtgt aaatcagcat tctcctttgc tgatgcaatt gcatgcggca
     9301 ggtatacgga ccggtgatgc agagcgaata ctgtccagcg gtgaatactg gcaacgtcag
     9361 acgacgctgc tgacagaaag ggaagtcagt tttatgaaag gactgttcag aattgtggat
     9421 atgacgcggt ggtatctgtg tccgcaggta cgggtcgcgg atatcgtcca gctgaacggg
     9481 aatatccggc cacgatcacg ccagtggtgg cagttattca ggatggtgtc tcagtggcat
     9541 gttgatgtgg ttatcgttga gctgcgttcg ttcagtattg ttgcagcagt agagctggat
     9601 gatgccagcc atttacgacc ggaacgcaga cgccgggata ttcttctgga agaggttctg
     9661 aggcaggctg gtattccgtt gctcagaagc cacgatgcca gaaaactgct gcagatgacc
     9721 ggagaatggc tgaatacaac aggggctgat cagcagtcac cggaacatcg tagctgacgc
     9781 cttcacgttg ctcagttgtc caaccccgga aacgggaaaa agcaagtttt cctcgctccc
     9841 ggcgtttcca taactgaaaa ccatactatt tcacagttta aatcacatta aacgacagta
     9901 atccccgttg atttgtgcgc caacacagat cttcgccaca attctcaagt cgctgatttc
     9961 aaaaaactgt agtatcctct gcgaaacgat ccctgtttga gtattgagga ggcgagatgt
    10021 cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta
    10081 agcctatgac tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca
    10141 aagagataaa agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga
    10201 gagaggggat aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac
    10261 tggatatact aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt
    10321 aaggaatttt atggctggcc acgccataag gtggcaggga actggttctg atgtagatgt
    10381 acaggagcca gaaaagcaaa aaccccgata atcttcttta actttggcga gtcagaaaga
    10441 ttaccggggc ccacttaaac cgtatagcca acaatcaagc tatgcgggga gtatagttat
    10501 atgcccggaa aagttcaaga cttctttctg tgctcgctcc ttctgcgcat tgtaagtgca
    10561 ggatggtgtg actgatcttc aacaaactta ttaccgccag gtaaagaacc cgaatccggt
    10621 gttcacaccc cgtgaaggtg cccggacgct tccgttctgc gggaaactga tggaaaaggc
    10681 ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg gcacatgccc gttcgcttgg
    10741 tctgcgtcgg cgcatgccac cggtactacg tcgccgggcc attgatgcgc tgctgcaggg
    10801 gctgtgtttt cactatgacc cactggccaa ccgcgtccag tgttccatca ccacgctggc
    10861 cattgagtgc ggactggcga cggagtctgc tgccggaaaa ctctccatca cccgggccac
    10921 ccgggccctg acgttccttg cagagctggg actgattacc taccagacgg aatatgaccc
    10981 gcttatcggg tgctacattc cgaccgatat cacgttcaca tctgcactgt ttgctgccct
    11041 cgatgtatca gaggaggcag tggccgccgc gcgccgcagt cgtgtggaat gggaaaacag
    11101 acagcgcaaa aagcaggggc tggataccct gggtatggat gaactgatgg cgaaagcctg
    11161 gcgttttgtt cgtgagcgtt tccgcagtta ccagacagag cttaagtccc gtggaatgaa
    11221 gcgtgcccgt gcgcgtcgtg atgcggacag gcagcgtcag gatattgtca ccctggtgaa
    11281 acggcaactg acgcgtgaaa tctcggaagg gcgcttcact gccagtcgtg aggcggtaaa
    11341 acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc gtaattacag
    11401 ccggctggcc acagcttccc cctgaaagtg acctcctcag aataatccgg cacgcgccgg
    11461 aggcattcgc acgcctgaag cccgccagtg cacaaaaaat cagcgctaca tacaaaaaac
    11521 aacctcatca tccaccttct ggtgcatccg gtcccccctg tttttgatac aaaacacgcc
    11581 tcacagacgg ggaattttgc ttatccacat taaactgcaa gggacttcac cataaggtta
    11641 caaccgttca cctcataaag cgccagccgt cagccctaca gggtgcaatg tatcttttaa
    11701 acacctgttt atatctcctt taaactactt aattacattc atttaaaaag aaaacctgtt
    11761 cactgcctgt ctgtggacag acagatatgc acctcccacc acaaacggcg ggccccgacc
    11821 gaagccggtt tagttacaac atgcaaacat aaacccctaa aaaacccaag tccgacgcag
    11881 aactgaaacc acaaagcccc tccctcataa ctgaaaagcg gccccgcccc ggcccgaagg
    11941 gccggaacag agtcgctttt aattatgaat gttgtaacta tattatcatc gctgtcagtc
    12001 ctctggctgg acgtatcgag tacacgctcg taagcggccc tcacggccct cacggcccgc
    12061 taacgcggag atacgccccg actgcgggta aacccttgtc gggctccgac cgcgcacaga
    12121 agctctgtca tagctgaaaa cgggtatggc ttggcagcta ccatgttctg tcaagaaaaa
    12181 tcgcttgaac tccgacgtac agccccctaa gtactgccag caagctgtca gcactaagtc
    12241 cccgatacag cgcggcatag ccgcgctaac ggggttaaaa gggcttacct atacttcatc
    12301 atcgctgaaa gcccgcatgg cctcgcaggg cagagatggg ataggtgaaa cctatcaata
    12361 cgtaccggct tacgccgggc ttcggcggtg ttactcctgt accatatgta actacatcat
    12421 gccgccttac atgcgctacg cgcatatgaa gacaaatagc ccacatcaaa cggtttttag
    12481 gtcttcagta cgccatagca ccagtgcacg agttttctca tcagcgcacc aatggcacac
    12541 attttggcct tacctctcag gcataatcgc tcatacatcg ctttcatcaa tgggttaaat
    12601 ctcagaccac acaacgaagc cagatacagt ttcgcccgga tttccggcgg accaattttt
    12661 gacagtttcg gacaccctct gaccgaggtg ccggaatatt tttcaacggg aacgacacct
    12721 aaaaatgcag ccgcctgt
//
LOCUS       DAJVTY010000124        12612 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVTY010000124 DAJVTY010000000
VERSION     DAJVTY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.124"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..1567
                     /locus_tag="OQQ66_003032"
                     /pseudo
     CDS             1..1567
                     /locus_tag="OQQ66_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            1903..2880
                     /gene="csiD"
                     /locus_tag="OQQ66_003033"
     CDS             1903..2880
                     /gene="csiD"
                     /locus_tag="OQQ66_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1905879.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2900..4168
                     /gene="lhgO"
                     /locus_tag="OQQ66_003034"
     CDS             2900..4168
                     /gene="lhgO"
                     /locus_tag="OQQ66_003034"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1905880.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            4191..5626
                     /gene="gabD"
                     /locus_tag="OQQ66_003035"
                     /pseudo
     CDS             4191..5626
                     /gene="gabD"
                     /locus_tag="OQQ66_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            5640..6906
                     /gene="gabT"
                     /locus_tag="OQQ66_003036"
                     /pseudo
     CDS             5640..6906
                     /gene="gabT"
                     /locus_tag="OQQ66_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7144..8505
                     /gene="gabP"
                     /locus_tag="OQQ66_003037"
     CDS             7144..8505
                     /gene="gabP"
                     /locus_tag="OQQ66_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1905881.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            8564..9226
                     /gene="csiR"
                     /locus_tag="OQQ66_003038"
     CDS             8564..9226
                     /gene="csiR"
                     /locus_tag="OQQ66_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1905882.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9227..9676)
                     /gene="kbp"
                     /locus_tag="OQQ66_003039"
     CDS             complement(9227..9676)
                     /gene="kbp"
                     /locus_tag="OQQ66_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1905883.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9760..9918)
                     /locus_tag="OQQ66_003040"
     CDS             complement(9760..9918)
                     /locus_tag="OQQ66_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1905884.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10101..10400
                     /locus_tag="OQQ66_003041"
     CDS             10101..10400
                     /locus_tag="OQQ66_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1905885.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10410..10934
                     /gene="ygaP"
                     /locus_tag="OQQ66_003042"
     CDS             10410..10934
                     /gene="ygaP"
                     /locus_tag="OQQ66_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1905886.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(10981..11385)
                     /gene="stpA"
                     /locus_tag="OQQ66_003043"
     CDS             complement(10981..11385)
                     /gene="stpA"
                     /locus_tag="OQQ66_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1905887.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12052..12501
                     /gene="alaE"
                     /locus_tag="OQQ66_003044"
     CDS             12052..12501
                     /gene="alaE"
                     /locus_tag="OQQ66_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1905888.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(12538..>12612)
                     /locus_tag="OQQ66_003045"
     CDS             complement(12538..>12612)
                     /locus_tag="OQQ66_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HCS1905889.1"
                     /translation="GIPHGFSSRVALERYLNGLFGEAS"
BASE COUNT         3147 a         3092 c         3228 g         3145 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc
      601 gtacgaggtt tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg
      661 ataaccccac cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca
      721 aaatatcaaa aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat
      781 gaacaacaaa tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct
      841 ttactcatga aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca
      901 aaaccgggtt ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac
      961 cacgattttc ctaacaatgg taatcatttt caatgacttt ctatttaacc atcaacaaga
     1021 tgaacaatta gcaatggcat atatggccgc tctcccgttc agcagacctt tagtttactg
     1081 ggatggccaa gtattaaaat caacgactga aattaaaaat tatgatgggt ccacgcgtgt
     1141 cggcggtgag gcgtggctta ataaaggttg ctctacctat cagcagctct acaatgaatt
     1201 ccacgcatta tatatagata aagcaggaat atggagcgca tttgagggtg tatttgcaac
     1261 taaaaacgtt ctggccttta gtcgtgggga ttctgtgaac attaatcact ctcctcatga
     1321 tgggctagtt ataataaata aaggaaacga agaagttgaa ggtacctggc ctaacaaatt
     1381 gcaacctgga atatacaaaa acatggggag taatagcgtt aacattatta ttaataatac
     1441 ccgaaaaatt atcccccctg gtaaagtatt tacgcttaga ggcggaactc taaatatcaa
     1501 tattcctggg cgtagcgctc ttcttttagg gaaaacagga gaaccgccga actatctcta
     1561 tttataattt aatgttatat ctgccccgat aaaacggggc agataatatg tttagtttac
     1621 taacggtcat tttgcagtga agccatttac tgttttttat cgaccagata atctgttctc
     1681 taatgttaac tccccctaac ctgttgcttt agttattcat ttcctgtctc actttgcctt
     1741 aataccctac gttaaatgtt actaatttgt tgtttttgat cacaataaga aaacaatatg
     1801 tcgcttttgt gcgcattttt cagaaatgta gatattttta gattatggct acgaaatgag
     1861 catcgccatg tcaccctaca tctcataaga ggatcgcttc tgatgaatgc actgatcgcc
     1921 gtacaaaata acgctgtcga ttcaggccag gactatagcg gattcactct catcccgtcg
     1981 gcgcaatccc cgcgtctgct ggaactcacc ttcaccgaac agacgaccaa acagtttctc
     2041 gaacaggttg ccgaatggcc cgtgcaggcg ctggagtaca aatcgtttct gcgttttcgg
     2101 gtaggcaaaa ttcttgacga tctgtgtgcg aatcagctgc aaccattgct gttgaagccc
     2161 ctgctaaacc gtgctgaagg tgcgctgttg atcaatgcgg tgggtatcga tgatgtggcg
     2221 caggcagatg agatggtgaa gctggcaacg gcggtagcgc atctgattgg tcgctcaaat
     2281 ttcgatgcga tgagcggtca gtattacgcg cgtttcgtgg tgaaaaatgt cgataactca
     2341 gacagctatc tgcgtcagcc gcaccgcgtg atggaactgc acaacgacgg cacgtatgtc
     2401 gaagagatca ccgattacgt gctgatgatg aaaatcgatg agcaaaacat gcagggtgga
     2461 aattcgttgc tgctgcatct cgatgactgg gaacatctgg accactattt ccgccatcca
     2521 ctggcgcgtc gcccgatgcg ctttgccgcg ccgccgagca aaaacgtcag caaagatgtt
     2581 ttccatccag tgttcgacgt tgatcaacag ggtcgcccgg tgatgcgcta tatcgaccag
     2641 ttcgtccagc caaaagattt tgaagaaggc gtgtggttga gcgagctttc cgatgccatt
     2701 gaaaccagca aaggcattct ttctgtaccc gttcccgttg gcaaattcct gttgataaac
     2761 aacctgttct ggctgcacgg tcgcgaccgc tttactccgc acccggatct gcgccgtgaa
     2821 ctgatgcgtc agcgtggcta tttcgcttac gccactcacc actaccagac gcatcagtaa
     2881 gcgcaaagga attgagcgga tgtatgattt tgtgattatt ggcggcggca tcatcggcat
     2941 gtcgaccgcc atgcaactga ttgatgttta tccggatgcc cgcattgcgt tgctggaaaa
     3001 agagtccggc ccggcctgtc accagacggg ccacaacagc ggcgtgatcc atgccggggt
     3061 ctattacacg cccggcagcc tgaaggcgca gttttgcctg gcgggaaacc gcgccaccaa
     3121 agccttttgc gatcaaaacg acattcgcta tgacaactgc ggcaagatgc tgatcgccac
     3181 gtccgatctc gaaatggaac ggatgcgcgc gttgtgggaa cgcacggcgg cgaacggtat
     3241 cgagcgcgag tggttaaacg ccgaggaact gcgcgagcgc gaaccgaata tcaccgggct
     3301 cggcggcatt tttgtgccgt ccagcggcat tgtcagctat cgcgaagtca cagcggcaat
     3361 ggcaaaaatc ttccaggcca gaggcggtga gattatttat aacgccgaag tcagcgccct
     3421 cagtgagcat aaaaacggcg tggtcgtccg aacccgtcag ggcggcgaat atgaagcatc
     3481 gacgctgatt agctgttccg ggctgatggc taaccggctg gtgaaaatgc tcggcctcga
     3541 accgggtttt attatctgcc cgttccgtgg cgagtatttc cgccttgcgc cggagcataa
     3601 ccagattgtt aaccatctga tttaccccat ccctgacccg gcaatgccgt ttttgggcgt
     3661 gcatctcacc cgcatgatcg acggcagcgt gaccgtcggg ccaaacgcgg tgctggcttt
     3721 taaacgcgaa ggctatcgca agcgcgactt ctcatttagc gacacgctgg aaattttagg
     3781 ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa
     3841 aaactcgctg tgcaaaagcg gctatctgcg gctggtgcaa aagtattgtc cccggctttc
     3901 gttaagcgat ctccagccct ggcccgccgg tgtgcgggcg caggcggtat cgccggacgg
     3961 caagctgatt gacgattttc tgtttgtcac caccccgcgc acgatccaca cctgcaatgc
     4021 gccctccccg gcagcgacat cagcaattcc tattggtgcg catattgtca gcaaggtaca
     4081 aacgctgttg gcaagccaga gtaaccccgg acgcacgctg cgagcgacac gtagtgtgga
     4141 tgccttacac gccgcattta atcaataacc tttgaaaaca ggacgtaaca atgaaactta
     4201 acgacagtaa cttattccgc cagcaggcgt tgattaacgg ggaatggctg gacgccaaca
     4261 atggcgggat catcgacgtc accaatccgg cgaacggcga caagctgggt agcgtgccga
     4321 aaatgggcgc tgatgaaacc cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgcca
     4381 ggcgtgcgct caccgccaaa gaacgcgcca acattctgcg caactggttc aatttgatga
     4441 tggagcatca ggacgattta gcgcgcctga tgaccctcga acagggcaaa ccactggccg
     4501 aagcgaaagg cgaaatcagc tacgctgcct cttttattga gtggtttgcc gaagaaggca
     4561 aacgtattta tggcgatacc attcccggtc atcaggccga taagcgcctg attgttatca
     4621 agcagccgat tggcgttacc gcagctatca cgccgtggaa cttcccggcg gcgatgatta
     4681 cccgtaaagc cggtccggcg ctggcggcag gctgcaccat ggtgctgaag cccgccagtc
     4741 agacaccgtt ctctgcgctg gcgctggcgg agctggcgat tcgcgcgggc attgcggctg
     4801 gcgtgtttaa cgtggtcacc ggttcggcag gcgcggtcgg taatgaactg accagcaacc
     4861 cgctggtacg caaactgtcg tttaccggtt cgaccgaaat tggccgacag ttaatggaac
     4921 agtgcgcgaa agacatcaaa aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg
     4981 tctttgacga tgccgatctc gataaagccg tggaaggcgc gctggcctcg aaattccgca
     5041 acgccgggca aacctgcgtc tgcgccaacc gtttggcgtg tatgaccgct ttgccgaaaa
     5101 attgcagcag gcggtgagca aactgcacat cggcgacggg ctggataaag gcgtcaccat
     5161 cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa gagcatattg ccgatgcgct
     5221 ggagaaaggt gcgcgtgtgg tttgcggcgc taaagcggat gaacgcggcg gcaacttctt
     5281 ccagccgacc attctggtgg acgtgccggc caacgccaaa gtgtcgaaag aagagacgtt
     5341 cggccccctc gccccgctgt tccgctttaa agatgaagcc gatgtgattg cgcaagccaa
     5401 tgacaccgag tttggccttg ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg
     5461 cgtgggcgaa gcgctggagt acggcatcgt cggcatcaat accggcatta tttccaatga
     5521 agtggccccg ttcggcggca tcaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg
     5581 catcgaagat tacttagaaa tcaaatatat gtgcatcggt ctttaactgg agaatgcgaa
     5641 tgaacagcaa taaagagtta atgcagcgcc gcagtcaggc gattccccgt ggcgttgggc
     5701 aaattcaccc gattttcgct gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc
     5761 gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa taccgggcac ctgcatccga
     5821 aggtggtggc cgcggtggaa gcgctgttga aaaaactgtc gcacacctgc ttccaagtgc
     5881 tggcttacga gccgtatctg gagctgtgcg agattatgaa tcagaaggtg ccgggcgatt
     5941 tcgccaagaa aacgctgctg gttacgaccg gttccgaagc ggtggaaaac gcggtaaaaa
     6001 tcgcccgcgc cgccaccaaa cgtagcggca ccatcgcttt tagcggcgcg tatcacgggc
     6061 gcacgcatta cacgctggcg ctgaccggca aggtgaatcc gtactctgcg ggcatggggc
     6121 tgatgccggg tcatgtttat cgcgcgcttt atccttgccc gctgcacggc ataagcgagg
     6181 atgacgctat cgccagcatc caccggatct tcaaaaatga tgccgcgccg gaagatatcg
     6241 ccgccatcgt gattgagccg gttcagggcg aaggcggttt ctacgccgcg tcgccagcct
     6301 ttatgcagcg tttacgcgct ctgtgtgacg agcacgggat catgctgatt gccgatgaag
     6361 tgcagagcgg cgcggggcgt accggcacgc tgtttgcggt ggagcagatg ggcgtggcgc
     6421 cggatcttac cacctttgcg aaatcgatcg ctggcgggcg tcaccgggcg cgcggaagtg
     6481 atggacgctg ttgcaccggg cggtctgggc ggcacctatg ccggtaaccc gattgcctgc
     6541 gtggctgcgc tggaagtgtt gaaggtgttc gagcaggaaa atctgctgca aaaagccaac
     6601 gatctggggc agaagctaaa agacggattg ttggcgatag ccgaaaaaca cccggagatc
     6661 ggcgacgtac gcgggctggg agcgatgatt gccatcgagc tgtttgaaga tggcgatcac
     6721 agcaaaccgg acgcaaaact caccgccggg atcgtggcac gcgcccgcga taagggcctg
     6781 attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtact gctcaccatt
     6841 gaagacgctc agatccgtca gggtctggag attatcggtc agtgttttga tgaggcgaag
     6901 cagtagcacc gtccccatgc aggaggcgac gctgcgcgtc ttgtccggcc tacgagaatc
     6961 caggtcggat aaggcgttta cgccgcatcc gacaatctgt acgtgaacag gaataaatct
     7021 atgtcggccg gataatgcgg agccgctctc cggcaaaaag aatcaataac aattaaacgc
     7081 gtgacccggc gcgggaaatg tcggggcgct ctcccaagtg acacactttc gagaggattc
     7141 aggatggggc aatcatcgca accacatgag ttaggcggcg ggctgaaatc acgccacgtc
     7201 accatgttgt ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc
     7261 atcgccgaag cgggcccggc agtattactg gcctatctgt tcgccgggct actggtggtt
     7321 atgattatgc ggatgttggc ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc
     7381 acctatgccg ataaagccat tggtcgctgg gcgggctata ccatcggctg gttgtactgg
     7441 tggttttggg tactggttat cccgctggaa gccaacatcg ccgccatgat cctgcactcg
     7501 tgggttccag gcattcccat ctggttattt tccctcgtca ttacccttgc cttaactggc
     7561 agtaatttat taagcgttaa aaactacggc gaatttgagt tctggctggc gctgtgcaaa
     7621 gtcatcgcta tcctggcctt tattttcctt ggtgcagtcg caattagcgg tttttaccct
     7681 tatgccgaag tgagcgggat ctcaagattg tgggatagcg gcggctttat gcccaacggc
     7741 ttcggtgcgg tattaagcgc gatgttgatc accatgttct cgtttatggg cgcagaaatt
     7801 gtcaccattg ccgccgcgga atccgacacg ccggaaaaac atattgtccg cgccactaac
     7861 tcggttatct ggcgtatttc tatcttctat ttgtgctcta tttttgtcgt cgtggcatta
     7921 attccgtgga atatgcccgg actaaaagcc gtcggttctt atcgctcggt actggaattg
     7981 ctcaatattc cccatgcgaa attaatcatg gactgtgtga tattactttc cgtaaccagc
     8041 tgcctgaact cggcgctgta taccgcttcc aggatgctct actccttaag ccgtcgcgga
     8101 gatgcgcctg cggtaatggg taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta
     8161 ctctccaccg gcgcggcatt cttaacggtg gtggtgaact attacgtgcc tgcgaaggta
     8221 tttaaatttc tgatcgacag ctccggcgct atcgccctgc tggtttattt agtcatcgcc
     8281 gtttcacagt tgcggatgcg caaaattctg cgagcagaag gaagcgaaat tcgcttgcgc
     8341 atgtggcttt atccctggct cacctggctg gtcatcggct ttattacctt tgtgttggta
     8401 gtgatgctat tccgcccggc gcaacagtta gaagtgatct ccaccgactt attagcaata
     8461 gggattatct gtaccgtgcc aattatggcg gctggaataa gctgataatg tggcaaaaaa
     8521 cacccgttca taatacgcgc tgataataat caggagtcac accatgacca ttacgtctct
     8581 ggatggctat cgctggctga agaacgatat tattcgcggt aattttcaac cggatgaaaa
     8641 attacggatg agtttgctga catcgcgtta tgcacttggc gttgggccgt tgcgggaagc
     8701 actttctcaa ctggtggcgg aacggctggt cacggtggtg aatcaaaaag ggtatcgggt
     8761 ggcgtctatg tcagagcagg agctgctcga tattttcgat gcccgcgcca atatggaagc
     8821 gatgttagtg agtctggcga ttgcccgcgg tagcgatgag tgggaggcag acgttctcgc
     8881 aaaagcgcat ctgctcagta agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga
     8941 gtgggatctg cgtcatcagg cgtttcatac agcaattgtg gcgggctgtg gttctcacta
     9001 tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgctatcgat ttatctggct
     9061 gcggcgaacg gtgctttcgg tagaaatgct ggaggataaa cacgatcagc accagaccct
     9121 gactgcggcg gtactggcgc gagataccgc gcgcgccagt gagttaatgc gccagcattt
     9181 actgacgcca attcccatta tccagcaggc gatggctggc aattaactac tcttccggaa
     9241 tacgcaacac ttgccccgga taaattttat ccgggctttt tagcatcggt ttattcgctt
     9301 cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag
     9361 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct
     9421 gatcatcaac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
     9481 cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca
     9541 ctttatcggc atcaggtata ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat
     9601 cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca tctttcacaa
     9661 aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
     9721 ggaacgcggc gcgttaacgc cgcggggggg agcacaggtt taatcgcggg tttgcaccca
     9781 gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc agcagaatat taataatgaa
     9841 cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga tggtgatgac
     9901 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
     9961 ttttatctct taagcgtagt aattttagcg gagcctgcca gtttcagctt acgcttttac
    10021 tctcatttac gctgctgagg ctggtatatg ttgcattttt agtttagagt ttacttaatt
    10081 tagaaaatac ttaaataaat atgactgaac tcgcgcaatt acaggccagt gccgaacagg
    10141 cagcggcctt attgaaagca atgagccatc ctaaacgatt gctgattctg tgcatgctta
    10201 gcggttcccc cggcaccagc gcgggagagc tgacgcgcat taccggactg agtgcctctg
    10261 cgacatcaca gcatctcgct cgtatgcggg acgaagggct tatcgacagc caacgggatg
    10321 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
    10381 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
    10441 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgacgct gatgaatatc
    10501 ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtgctggaa cagtcaggtc
    10561 ttccacctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
    10621 gtaataacgc cgataaatta gcagcgattg ccgcccccgc agaaatattt ttactcgaag
    10681 ataggattga tggctggaaa agagctggat tgccagtagc ggtaaataaa tctcaaccct
    10741 tgccgttaat gcgccaggtg cagatcgctg cgggtggttt aatattaatc ggcgttgtac
    10801 tgggttatac cgtaaatagc ggtttcttct tattaagtgg ctttgttggt gccgggttac
    10861 tgtttgcagg aatcagcggt ttttgtggaa tggcaaggtt gttagataag atgccgtgga
    10921 accaacgagc ttgagaagcg acgccggacg cgccctagca gcgacatccg gcctcagtaa
    10981 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcaa ttggcttcgg
    11041 tgtacggccc tgaccggtcc aggtcttagt ttcaccgtta acatcagtga atttatattt
    11101 cgccggacgc ggctggcgtt ttttaccagc acgtggtgca gcagcagagc tattacccaa
    11161 taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc taattttttc
    11221 ctgacgctca gccagttcac gctgctgctg ttcttcttct tcacgtcttt ctttagtgac
    11281 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
    11341 acggagggtt cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
    11401 ttaacgccaa aacaaaaaag tatttcgtcg cgtagtgtaa gcgatcactt attatatttc
    11461 cacaaaattt aatctattta cagtcctgcg cgagattatt tcagctgtta ttttctgaaa
    11521 ctctcagaat aatttcaaga ttatatatta gccattttga tgagtaattt aggttattgg
    11581 tgaacagagt ggcgctctct gctcgctaac tccgcaagcc tgataaaacc gaagtgaata
    11641 aaaatgcaat ataacagaat aaaagaaaac acccctcact ggtgctttct gctgttaatg
    11701 tacattttct ggcatcaaaa atgctgtaaa tgacccggtc acgacaaaca ccgaattctc
    11761 tacgcttata gcgttacctc acccccaaac tttcattagt gaaattaaag gcaaggtcat
    11821 atccaattga tttaattgaa aataaatcat atcggcatgt aaaaatccac aatgtacaaa
    11881 aaacgagctg ttacggaata ttttatctac aaaaactgac taaataaaag tttttcacta
    11941 attgattagt catagccagc gatatacgct atgcgaaaat gcagatggca atgagatcca
    12001 ctgctttcat ctccattaac atcccattac gctttattaa ggagcattag catgttctca
    12061 ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt ttactgttct
    12121 gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca gtctttttat
    12181 tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg tatgtaccgc
    12241 gatctgttta tgcgcgcggc acgcaaagtt agcccgtcgg gctggataaa aaatctggct
    12301 gatatcctgg cttatgtgac gttccagtca ccggtgtatg tggcgatctt gttagtggtg
    12361 ggcgcagact ggcatcagat tatggcggcg gtcagttcaa acatcgttgt ttcgatgttg
    12421 atgggggcgg tttatggcta cttcctcgat tattgccgcc gactgtttaa agtcagccgt
    12481 taccagcagg taaaagcctg actcttcttt tcgcgactgg catcgccaga cgcgtcttta
    12541 actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg aactgaagcc
    12601 atgcgggata cc
//
LOCUS       DAJVTY010000125        12047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVTY010000125 DAJVTY010000000
VERSION     DAJVTY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12047
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.125"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(354..692)
                     /gene="osmE"
                     /locus_tag="OQQ66_003046"
     CDS             complement(354..692)
                     /gene="osmE"
                     /locus_tag="OQQ66_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1905890.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            894..1721
                     /gene="nadE"
                     /locus_tag="OQQ66_003047"
     CDS             894..1721
                     /gene="nadE"
                     /locus_tag="OQQ66_003047"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1905891.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            1951..2838
                     /gene="cho"
                     /locus_tag="OQQ66_003048"
     CDS             1951..2838
                     /gene="cho"
                     /locus_tag="OQQ66_003048"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1905892.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2798..3373)
                     /gene="ves"
                     /locus_tag="OQQ66_003049"
     CDS             complement(2798..3373)
                     /gene="ves"
                     /locus_tag="OQQ66_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1905893.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3576..4046)
                     /gene="spy"
                     /locus_tag="OQQ66_003050"
     CDS             complement(3576..4046)
                     /gene="spy"
                     /locus_tag="OQQ66_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1905894.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4376..5344)
                     /gene="astE"
                     /locus_tag="OQQ66_003051"
     CDS             complement(4376..5344)
                     /gene="astE"
                     /locus_tag="OQQ66_003051"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1905895.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGDETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5337..6680)
                     /gene="astB"
                     /locus_tag="OQQ66_003052"
     CDS             complement(5337..6680)
                     /gene="astB"
                     /locus_tag="OQQ66_003052"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1905896.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6677..8155)
                     /gene="astD"
                     /locus_tag="OQQ66_003053"
     CDS             complement(6677..8155)
                     /gene="astD"
                     /locus_tag="OQQ66_003053"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1905897.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8152..9186)
                     /gene="astA"
                     /locus_tag="OQQ66_003054"
     CDS             complement(8152..9186)
                     /gene="astA"
                     /locus_tag="OQQ66_003054"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1905898.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9183..10403)
                     /gene="astC"
                     /locus_tag="OQQ66_003055"
                     /pseudo
     CDS             complement(9183..10403)
                     /gene="astC"
                     /locus_tag="OQQ66_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            10849..11655
                     /gene="xthA"
                     /locus_tag="OQQ66_003056"
     CDS             10849..11655
                     /gene="xthA"
                     /locus_tag="OQQ66_003056"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1905899.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11822..>12047
                     /locus_tag="OQQ66_003057"
     CDS             11822..>12047
                     /locus_tag="OQQ66_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1905900.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIV"
BASE COUNT         2935 a         3259 c         2976 g         2877 t
ORIGIN      
        1 agtaaagagg tagacatgcc cgcagaacaa aacagataaa tgtgtttctt ttccataaaa
       61 ctgccctcat cgacgattat ctgtcagcca gacactccgc aagccttaac ctgcttccat
      121 gctctgggta acttgcgaaa ccaacatgac tttttttgtg gctgaaagga gtatacggta
      181 tagacctgag caatagtatt tctttgacct tataaattgt ctctccttga cctgcggtta
      241 tgaccctctt cacatttcgg gcaaataatt cgcgaagata attaagaact caggccaaaa
      301 aaaccggcgc aatggccggt ttccgttgtt actcaagctt tcaaacgaag tgattacttc
      361 gcagcctgtg gatcagtgtc gtattcagca caggtctgat aaccggagtt gatgacatgt
      421 ccggtatcat ctaacgcgac aaagtaggtt tctgctttac catcacgttg acccaggatg
      481 taggtctggc aagtaccacg agcatggatc atgctcactt cagacgaagg tttacccgca
      541 atttgtgcaa cctgcgcccg gctcatgcct tttttgacgt ctttcaccac aggctgtaca
      601 aactggtctt tggtacgatc ataagccgta caacccgcca gcatggttaa taccgccgct
      661 gcactcagaa ttcctgccat attcttgttc atattccgtc ctcttgttta tcagcgtgtt
      721 agataagcct ggaatacatt gggcgctttt tcaagcccgt gaacgaaacg gctccgcttt
      781 cagaggattc ctgtatgacg ttttatccac cattcaaccc gctgtcgctt gtcgtttcag
      841 tagcgacggg ttagctttaa ggaagttttg tcttttctgt ctggaggggt tcaatgacat
      901 tgcaacaaca aataataaag gcgctgggcg caaaaccgca gattaatgct gaagaggaaa
      961 ttcgtcgtag tgtcgatttt ctgaaaagct acctgcaaac ttatccgttc attaaatcac
     1021 tggtgctcgg gatcagcggc ggtcaggact ccacgcttgc cggtaagctg tgccagatgg
     1081 cgattaatga gctgcgccag gagaccggca acgaatcact gcaatttatt gccgtacgcc
     1141 tgccctatgg tgttcaggcc gacgaacaag attgccagga tgccattgcc tttattcaac
     1201 cggatcgcgt attaaccgtt aatatcaagg gcgcggtatt ggccagcgaa caggcgctgc
     1261 gcgaagcggg cattgaactg agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga
     1321 tgaaagcaca atatagcatt gcgggtatga ccagcggtgt cgtggtgggc accgatcatg
     1381 cagcagaagc tattaccgga ttcttcacta aatatggtga cggcggtacg gacattaatc
     1441 cgctgtatcg tctcaacaaa cgtcagggta aacagttact ggcggcatta gcttgcccgg
     1501 aacaccttta taagaaagcg ccaacggccg atctggaaga tgatcgccct tctctgccag
     1561 atgaagtggc actcggcgtg acctatgaca atatcgacga ctatctggaa gggaaaaacg
     1621 tacctcaaca ggtcgccaga acaatagaga actggtatct gaaaaccgaa cataaacgcc
     1681 gtccgccaat taccgttttc gatgatttct ggaaaaagta ataatttgca catattggat
     1741 tgtgcgaaaa agagtaattt gttcacgccg gatgcggcgt gaacgcctta ttcgacctat
     1801 aaaactatgc aaattcaata cattgcagga gtcgaatagg cctgacaggc gtagcacgtc
     1861 agacggtgta acctttgtca tcgacccgcc tcttttttaa tcgcttcccg cctgttacac
     1921 tggatagata accagcattc ggagtcaaca gtggtacggc gtttaacttc tccgcggctc
     1981 gaatttgaag ctgcggcaat ttatgaatat cccgaacatt tacgttcatt ccttaatgac
     2041 ttacccaccc gaccaggggt gtatctgttt catggtgaaa gtgacaccat gccgctctat
     2101 atcggcaaaa gcgttaacat ccgcagccgc gtcctttctc atttacgtac cccggatgaa
     2161 gccgccatgc tacggcaatc ccgacggatc agctggatat gtaccgccgg tgaaatcggc
     2221 gctctgctcc ttgaagcgcg attaatcaaa gaacaacagc cgctgtttaa taaacggttg
     2281 cgccgcaatc gtcagctctg tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat
     2341 gccaaagagg tggatttttc acgagccccc aacctgtttg gcctgtttgc caataggcgc
     2401 gcagctttgc aagcattgca gaccatcgct gatgaacaaa aactttgtta tggcctgctg
     2461 ggactggaac cgttaagtcg cggtcgtgca tgttttcgtt cagcgctaaa acgttgcgcc
     2521 ggagcatgct gcggtaaaga gagccatgag gaacatgcgc tacgcttgcg ccagtctctg
     2581 gagcgtttgc gggtggtgtg ttggccttgg caaggggcgg tggcgctgaa agaacagcac
     2641 ccggaaatga ctcaatatca tattattcaa aactggctgt ggctgggggc ggttaattcg
     2701 ctggaagacg cgacaacgtt aattcggaca cccgccgggt ttgatcacga cggttataaa
     2761 attctttgta agccgctgct ttccggtaac tatgaaatta ctgaacttga tccggcgaat
     2821 gaccagcgag ccagttgatt tcactgaaca acagtttacc ttgcggttgt aaaagccgca
     2881 gggtgtgcct gccatcgaac cagcacgcgc cctggtcggt agtgagcaat ttatcgccca
     2941 gttgccacgc cccgttaatc acaaacacca ccccgccgcg tgagccaaag gtagtaaacg
     3001 tacgttcggc aattctgact ttcgccttac agacatccag gcgtgtcatg atgttgaaat
     3061 ccatcgacat ctgcccttcc gtcagtttcg cttttaccac ctggtccgct gcaaaggcaa
     3121 aaggctgtaa cggttttaag gtatggttaa agcggtctgc gctttcaagg agcatctcgc
     3181 cgccttccag caacgtcact atcctttcca tgccgggaaa caaagaaaac tccccattcg
     3241 ccgcgatgga tgcaatgctg gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc
     3301 aaatttcgcg cgtttcaccg gcagcatttc gccacagatt caccgacatt ttacgcatat
     3361 caaagtattc catactcgct cccggcccgt tcttttcatt gttatcgttc aggtaaccgt
     3421 ggatggttat cggcaactcg ggtgatttac gttagtggtg atcaggcttt ttttacaact
     3481 gtttgagaag agaaaagaaa accgccgatc ctgtccaccg cattactgca aggtagtgga
     3541 caagaccggc ggtcttaagt tttttggctg aaagattatt cagcagttgc aggcatttta
     3601 ccttttgccg ctggacgttc tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc
     3661 tgttccggcg tcaggatgtt gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta
     3721 gctttgcgct gttcttccat ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta
     3781 tcgctgacaa tgatgtcatg cattgcgcgg cgttcttcca gcggcggacg tttcatctgg
     3841 tcacgctggc ctttcatgat ttcgcggatc tgctgtttct gcgcgtcggt caggttcagg
     3901 tctttgaaca tcatgtcctg atgcggaccg aacttgcctt tgtggtgcat catcggcttc
     3961 gcgtcagccg gtgctgcggc atgggccagg ttagccgcgc caagagccag ggtagaggca
     4021 acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta tttattacgg
     4081 ctttcttaag tagcgtgccg tgttgacaag attaacttta ctgactttag cgtcaattaa
     4141 tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa
     4201 aaaagaaaat ttgatggaga gtgatgagag aatattacaa cacaatgatt ttgcagagat
     4261 tatgaagaac tatactggat gactggtgat aaataaagca aataaccagg attaatctgt
     4321 attaatttat aagaaagcaa cttaataccc gcagaatgat ttctgcgggt aagtattagc
     4381 ttattttttc gagcattaat cccgcgcgta atcccaacgc taccaacgga ttagggaata
     4441 acacatactc tacatcatgg gttacggtaa aacgttcctc tccgtcctgc gccagcaatg
     4501 ttcctttctc aaacggcata aaattcagcg tgtcacttgc catatgcatt tcgaaggacg
     4561 gcgagtggcg agtaatttgc gaaaccaccc gataacggag cggcggtgtt ctcacgatac
     4621 cgacactctc accagatagc agcgcagcaa ttgcgctggc agttactgca aactggcgaa
     4681 gatcgttttg cccaaagggc aacgctttgc caagttccag cgtacaggcc agcgcgccaa
     4741 aatgtctggc gctgaaatgg gtaaacgtac caccaggttc ctgatggaac accagcgcct
     4801 ccagccccgc cgcacccagc cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg
     4861 gtaatacacc gaactgcgga tgcaaggagc cacgaattgc ggtatgtaga tcaaggtgcc
     4921 agcgcacaga ttctttgccc tggtcataaa aatcttccag gcactgttcc agttcgcgcg
     4981 cccgacaggt ttctccgctt tcagcaaata gctgccaacg accgccaaac attcgattca
     5041 tatcgctatg gcaataacgt ttcccttgct tcagcgcagg aggattcccg aggatcacca
     5101 gcaaccgcca acgtaacggg atctcgccgt gagatatcgc gccaagcaac gcgtccagca
     5161 tttccacagg tgccgtctca tcaccgtgga tccccgcaga aatcaccagc gcgccttgcg
     5221 gtggcgttaa tggcgtcagt tccagcacac catcgcccag ccagcgccag cgaacgccgt
     5281 tgatttctcg ctcggtgata accggttttt tacccgttaa ggtcagagca agaaaattat
     5341 ccattgcccc ctccctcgcg ctggaacgga taaaccgaac cgagattcag taattggctc
     5401 aatacatcca gtgcttcccg cccttcgcgc agcaattgcg ggtcggccag atcggcagca
     5461 gtaagacgat cgcggtagta acgatccacc cagtcattga gcgcattaaa caaggtatcg
     5521 ttcatcatca ccgccggatt caccgcccgg cgttcttctt ctgtcaatac cacccgcaac
     5581 cgcaggcacg ccgggccgcc gccattcgcc atgctttcac gtaagtcaaa gacttttagt
     5641 tcgctaatcg ggttgtcagc ggcaaggagt tcattgagat aaccccatac tccggcgtgt
     5701 tcccgacact cctgaggcag caccaacatc atggaaccat catcgcggct caacagttgg
     5761 ctgttaaaca gataggtaga caccgcatcc gacacggaaa cctgagttgc cggaacttct
     5821 atcgccataa aaccgttcac ccgcgcccgc aggtttgcca gtaactgtga ctggcgagcg
     5881 aacgcctgtt ggtggcaaaa cagcacctgg cggttactta cggcaatcac gtcattatga
     5941 aaaacaccct ggtcgataac gtccgggttt tgctgggcga aaatcacctg ttggggattc
     6001 acctgattaa gccttgccac cgcctcgctg gcttcgcgag tctgtcgcgc cggataacgg
     6061 gaaggccggg tatcattgcc ttcttctcgc ccgtagacaa aaagttgcat acccggctca
     6121 ccgtaatgac cgccgagacg attgtggttt gccgccccct catcaccgag caacgctacc
     6181 tgcggcaacg ccgaatggac gctaaatttc tcttcgtcgt taaaaatagc ttttaacagc
     6241 gattcagtga cgggcgcttc cagcgaacgg tgaaatttat tgttcaggtt ggcaacggtg
     6301 agatgcactt tgccatccag cgtatcggca gatggcgcga tcgttgccgc attggctacc
     6361 cacattggcg aagcggagct gacgctggaa agccagtgcg gtgcctggcg tgcaactttt
     6421 tccagtacct gctcatcgct accactgaat cccaactgac gcagcaccgg aataaacgga
     6481 cgctcgtgcg gcgggatcac ggcctggggg aatcctgcat cggcaagggt tttcattttc
     6541 agtaagccct gtttcgccgc cagtcgcggg ttagacacct gaaaacggtg acgggtagag
     6601 gcttcattac caaacgacag gccagcgtaa tgatgcgtca gccctaccag cccgtcgaaa
     6661 ttaacttccc aggcgttcat cgcaccacct catcggaaaa atccagcccg gggttaagcg
     6721 ttgcgggcaa agttaacgag tccgactcca ggctcgccat aggccatgcg cagtaatctg
     6781 cggcatacca ggcgctgggg cgatggttac cggatgcacc aatgccgccg aatggcgcgg
     6841 tactggcagc accagtaagc ggtttgttcc agttaacaat ccccgcccgc gcctccagca
     6901 acagttgatc gaatttttcc cgttcggggg aaaccagacc gcaagagagt ccgaagcgag
     6961 tgttattcgc cattagaatc gcttcctcga aagaatcata acgccagacg cgcaataacg
     7021 gtccgaacac ctcttcatct ggtacgccag caacgcctgt catttcaatg atccccggcg
     7081 tcagcaacga tgtctctaat tgtaataagc gcggcgcaag cagggttcgt ccgcccatcg
     7141 cttccagttg ctgccaggca gtaaccacct gctgtgcggc ctgttcagaa atcagcccgc
     7201 caataaacgg ctgcggttcg tcatcccagt tgcccggcgt taatcgctgg ctgacggcaa
     7261 ccagacgagc aagaaacgca tcgccctgcg ctccgctttt gagcaataaa cggcgggcgc
     7321 aggtgcagcg ttgcccggct gtgacaaacg ccgactgaat ggtcagatgg acagccgcgt
     7381 cgatatccgc cacctcatcg ataattagcg gattattacc gcccatctca agggcgagaa
     7441 ttttctccgg ctgaccggag agctggcgat gcagctggta gccggtattg gcgcgaccgg
     7501 taaacagcaa accgtcgaga tcctccagcg cactcagcgc ctgacccgtt tcacgcccgc
     7561 cctgcaccag gttcagcacg cccggcggca agccagcctg ctgccataaa cgcattaccg
     7621 cttcgccact ccacggtgtc agttcgctgg gtttaaagat aatggtgtta cctgccagca
     7681 atgccggaac gatatgtccg ttcggcaaat gaccagggaa attatacggc ccaaacaccg
     7741 ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcacc gtccggcatt tcactacgct
     7801 gctcgccggt acgaacgtga tacgccttaa ttgatatcgc gattttattg atcatcgccg
     7861 tcacttcggt tgccgcttcc cagcgcagct tacccgtttc tctggcaata atcgcggtta
     7921 attcggcttt attgctttcc agcaatccgg caaagcgttc gacaacgacc tgacgttcag
     7981 ccaatgagag ccgcgcccag cgcggaaacg ccgcacgggc tgcccgacaa gcctgttcga
     8041 cctgagcggc atcggcatca ttgccttgcc ataacacctc gcccgatacc ggattacgct
     8101 tcacacgcga tgcgccctgg cccgttatcc agtcaccgtt aatccataaa gtcatgctgt
     8161 tttctccact gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc atttgagggc
     8221 atccagttgt gcggcggtta aaatcaaacg ctcggttgcc ggatcggtac gcgccagcac
     8281 cacgcggaaa tggtgataat tttcattggc gaccaggcag gccggaaaat cgccctgcgc
     8341 aggctgccct tctgccactt ccaccagccg acttttacgg atggcgcgca cgcggtcgat
     8401 gtcacactca agcgttggcc cgccgtcgaa gatgtcgata tagttgcggt aacgaaaacc
     8461 ttctttctcc agcaccgcgc gggcaggcgc ggtttgcgga tgtacctgac cgatgacgtc
     8521 ctgggcttcc tgggataaaa agtgggtata gatcggatgt ttcggcatca gttctgcaat
     8581 aaatgccttt tgcccggtgc cgcagagaaa atcggcgcgg ctaaaatcca tcgaaaagaa
     8641 gcgtttaccg aggctttgcc agaacggtga atagccgtgt tcgtcaatca ccccgcgcat
     8701 ttcagcaacc actttgtcat taaacttgtc gcgaaaagcc gccataaaca taaagcgcga
     8761 tttcgacagc aaatagccgt tgccctcttt gcgccagtcc gggtcgagaa acagcgtgca
     8821 cagctcgctg ctgccggtgt gatcgttact aagaaacagc gtcggcaatg cgttatagac
     8881 attcagctct tttgaggcgt gaaccaacgt gccgacccga tagttgtgcc agggatcgtt
     8941 cagcccaacc gccacctcaa tggcgcaaat ccccgccacg gtgcctgtct cgctatcttc
     9001 cagcacgaac acatagccct gctcgctttt gggcagttcg ccttgccagg ttttgattgc
     9061 cctttcgata cgcgcggaaa gcgtggcttc attagcggga agcgacgtca ggccgccgcc
     9121 cgttttgctg gcaagctgca tcagcgccga gacatctgag cgctcaacgg gacggatgac
     9181 catcatgatg aacctcggct aacaaaatgt tcgcaagcag ctgcaaagcg atccagtccg
     9241 gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa aacgcaccac gttgccaccc
     9301 gcaatcagta ccataacgcc tgctttcgcc gcctcctgag agatctgttt cgcttgtccg
     9361 gcgtaatcgg cattcagtac acagccaatc aacaaaccta agccgcgaac ttcactgaac
     9421 aaaccgtagc ggtgattaat ggtattaaga cgctcaacaa accagtcgtg acgctgttta
     9481 acgccattaa gcatctctgg tgtgttgatg agctccagca ctttgcccgc caccgccgag
     9541 gccagcggat tgccgccata ggtggtgcca tgagtgccaa cggtcatcac gctggcgcac
     9601 tcttcggttg ttaacaacgc accgaccggg aaatcgccgc ccagcgcttt ggcggtagtt
     9661 aacagatcag gtgtcacgcc gtagtgcata taggcataca gttccccggt gcgcccgacg
     9721 ccggtttgta cttcatcaaa aatcaacagc gcattgtggc ggtcacacaa ttcacgcaga
     9781 ccttgtaaaa acgcgttgct ggctggcacc acaccgcctt ccccctggat gggttagaca
     9841 atcaccgcac aggtggcgtc gtcaatcagc gcgctggcag agttaatatc gttatatgca
     9901 gcatgacgga tatctggtgg cagtggcgca aaatcctgtg aataggctgg ctgcccaccc
     9961 gcactgacag taaacagcgt gcgaccatga aacgcatttt taaacgccat gataccgctc
    10021 ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc cgcttcgttg
    10081 gcttccgcac cggagttaca aaagaagacg cgatcggcaa acgtggcgtc gatcaatttt
    10141 ttcgccagtc gcagtaccgg ctcgttggtg taaccgttgc cggtatgcca gaacttactc
    10201 gcctgttcgt tcagcgcttc acgcagttcc ggatgcgcat ggcccagcgc gttcaccgca
    10261 atgccacccg cgaagtcgat atactctttc ccctgctgat cccacaagcg cgaaccttcg
    10321 ccacgtaccg gtataaaggg tgccggagcg taaacaggta tcatccattc atcaaagttt
    10381 tcacgcgtaa ttggctgaga catagcgacc tctacagtaa ataattcgtt atttatatgt
    10441 taacaataag taatgtttgc gctgtaaatg tagattgcag ggttcgtgcc agccagtgat
    10501 aaaagtgcat aaacggcgga ggctaactgg aaatcaagga gttataacca aaccatatgc
    10561 atttaaagtg catataaagt gaatacgttt gcgatgtggg tgaataaaaa gaataaaaaa
    10621 cgcaatgtta tgcagaagta aaatataatt ctggaattgt gatcattgac gaaatttact
    10681 ggaaattact gcgccattct gacgcagcgc gcaccaaaag cgggcatttt ttgcgccatc
    10741 gttgacatca ttaacaacca tcgatcaaat cacttaacaa caggcggtaa gcaacgcgaa
    10801 attctgctac catccacgca ctctttatct gaataaatgg cagcgactat gaaatttgtc
    10861 tcttttaata tcaacggcct gcgcgccaga cctcaccagc ttgaagccat cgtcgaaaag
    10921 caccaaccgg atgtgattgg cctgcaggag acaaaagttc atgacgatat gtttccgctc
    10981 gaagaggtgg cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg ccattatggc
    11041 gtggcgctgc tgaccaaaga gacgccgatt gccgtgcgtc gcggctttcc cggtgacgac
    11101 gaagaggcgc agcggcggat tattatggcg gaaatcccct cactgctggg taatgtcacc
    11161 gtgatcaacg gttacttccc gcagggtgaa agccgcgacc atccgataaa attcccggca
    11221 aaagcgcagt tttatcagaa tctgcaaaac tacctggaaa ccgaactcaa acgtgataat
    11281 ccggtactga ttatgggcga tatgaatatc agccctacag atctggatat cggcattggc
    11341 gaagaaaacc gtaagcgctg gctgcgtacc ggtaaatgct ctttcctgcc ggaagagcgc
    11401 gaatggatgg acaggctgat gagctggggg ttggtcgata ccttccgcca tgctaatccg
    11461 caaacagcag atcgtttctc atggtttgat taccgctcaa aaggttttga cgataaccgt
    11521 ggtctgcgca tcgacctgct gctcgccagc caaccgctgg cagaatgttg cgtagaaacc
    11581 ggcatcgact atgaaattcg cagcatggaa aaaccgtccg atcacgcccc cgtctgggcg
    11641 accttccgcc gctaatttag cagctctcct ggctcaaact gggtcaggag aattaacctt
    11701 gagaaaaatc aacaaactgt cagtaatgat ttgttgcctg ccgtcctttg ttataccgtc
    11761 actgcgtttt tagttgtctg accacttctc tattatcaag tttgatatag gaaactccac
    11821 gatgaatgct gagcgtaaat ttcttttcgc ctgtcttatt tttgcgctgg tcatttacgc
    11881 tatccacgct ttcggtttat tcgatctgct caccgattta ccccacttac agacactcat
    11941 tcgccagagc ggattgttcg gttatagcct ctatattctg ttattcatca ttgccaccct
    12001 ctttctgtta ccaggaagca tactggtgat cgctggagga atagttt
//
LOCUS       DAJVTY010000126        12017 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVTY010000126 DAJVTY010000000
VERSION     DAJVTY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12017
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.126"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..400)
                     /locus_tag="OQQ66_003058"
     CDS             complement(<1..400)
                     /locus_tag="OQQ66_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1905901.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDG"
     gene            556..1596
                     /gene="gpr"
                     /locus_tag="OQQ66_003059"
     CDS             556..1596
                     /gene="gpr"
                     /locus_tag="OQQ66_003059"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1905902.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1636..2130)
                     /locus_tag="OQQ66_003060"
     CDS             complement(1636..2130)
                     /locus_tag="OQQ66_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1905903.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2321..3205
                     /gene="yghA"
                     /locus_tag="OQQ66_003061"
     CDS             2321..3205
                     /gene="yghA"
                     /locus_tag="OQQ66_003061"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1905904.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGQAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3477..3902)
                     /gene="exbD"
                     /locus_tag="OQQ66_003062"
     CDS             complement(3477..3902)
                     /gene="exbD"
                     /locus_tag="OQQ66_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1905905.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3909..4643)
                     /gene="exbB"
                     /locus_tag="OQQ66_003063"
     CDS             complement(3909..4643)
                     /gene="exbB"
                     /locus_tag="OQQ66_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1905906.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4895..6082
                     /gene="metC"
                     /locus_tag="OQQ66_003064"
     CDS             4895..6082
                     /gene="metC"
                     /locus_tag="OQQ66_003064"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1905907.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6222..6881
                     /locus_tag="OQQ66_003065"
     CDS             6222..6881
                     /locus_tag="OQQ66_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1905908.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6921..7820)
                     /gene="yqhC"
                     /locus_tag="OQQ66_003066"
     CDS             complement(6921..7820)
                     /gene="yqhC"
                     /locus_tag="OQQ66_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1905909.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8014..9177
                     /gene="yqhD"
                     /locus_tag="OQQ66_003067"
     CDS             8014..9177
                     /gene="yqhD"
                     /locus_tag="OQQ66_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1905910.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9282..10109
                     /gene="dkgA"
                     /locus_tag="OQQ66_003068"
     CDS             9282..10109
                     /gene="dkgA"
                     /locus_tag="OQQ66_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1905911.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVIIAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10309..11235
                     /locus_tag="OQQ66_003069"
     CDS             10309..11235
                     /locus_tag="OQQ66_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1905912.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11286..11543
                     /gene="yqhH"
                     /locus_tag="OQQ66_003070"
     CDS             11286..11543
                     /gene="yqhH"
                     /locus_tag="OQQ66_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1905913.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            11623..>12017
                     /locus_tag="OQQ66_003071"
     CDS             11623..>12017
                     /locus_tag="OQQ66_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905914.1"
                     /translation="MRRWHTCFFILLFCGFSGNVLCKESINEAIERLYQPLTSNDDIP
                     ITDEFSLDLRSNMSATPGKMTPWLNNNLLCRCVDDAFVSAEIIQSTHLNGGRTHVLLQ
                     LVIKQNRTDIHRLLGLMLVRENDNHRWLI"
BASE COUNT         3108 a         3049 c         2997 g         2863 t
ORIGIN      
        1 agccgtcagg tgccagggcg atataccccg ccttcgtcac tcgccgtgcc acatcttcga
       61 tatacggatt cagtccacga ttctcatgca ccaccaccac ggctggcgtt ttgccgctca
      121 tttttgcggg tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg
      181 tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgtc agcacataat
      241 ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca tacttcgccg
      301 caaggttgag gaactcacgt ttcgaaattt tcccgtgagc gtaatagtcg tagtaatcca
      361 acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttgcattc ctcaactggt
      421 gttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca
      481 gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga aattgaacgg
      541 tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca gatgcagtac
      601 cgctattgcg gaaaaagtgg tttacgcctg cccgcgttat cgctcggttt atggcacaat
      661 ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta
      721 ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag
      781 aactttggtc gcctgctgcg ggaggatttt gccgcttatc gcgatgaact gattatctct
      841 accaaggctg gctacgatat gtggcccggc ccttacggct ctggcggttc acgtaaatac
      901 ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt cgatatcttt
      961 tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat
     1021 gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg
     1081 caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca tcaaccttcg
     1141 tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac
     1201 ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc
     1261 aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg tggtctgacg
     1321 ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag
     1381 cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg
     1441 acgtcggtat tgattggtgt cagccgcgcg gagcaactag aggagaacgt gcaggcgctg
     1501 aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc
     1561 gagctgaatc tgtggcaggc gtcttccgat aaatgacctg ttaataacgg gcgcgcgaac
     1621 cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa
     1681 tgcagagagc acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac
     1741 atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc
     1801 tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca gcttctcttt
     1861 gttctcggag atatccagct gcgagacgaa attctcataa ccggaaaaca tcaccatcac
     1921 cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga
     1981 ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc
     2041 cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc
     2101 atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa
     2161 cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac gttaaacctt
     2221 tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg
     2281 tctaccctta aaagacgttg aacctgaagg gagaaaaaca atgtctcatt taaaagaccc
     2341 gactacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca
     2401 ggcgaagatg acaccggtac cggattgcgg cgagaaaacc tatgttggta gcggtcgcct
     2461 gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg ctgccgccat
     2521 cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga
     2581 cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg
     2641 cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca aggcgttagg
     2701 cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg atattgcaga
     2761 cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc tgttctggct
     2821 aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat
     2881 ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg cggcgattct
     2941 gaactacagc cgtggccagg caaaacaggt cgcggagaaa ggtattcggg tgaatattgt
     3001 cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc aggataagat
     3061 cccgcagttt ggtcagcaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc
     3121 tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggtgt
     3181 gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca
     3241 ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa
     3301 gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag
     3361 cacctaatgc cggatgcggc gtgaacgcct tatccggcct acaaacgcac gaagattcaa
     3421 catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat tctactttac
     3481 ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc
     3541 gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tatccgctcg gaagaagatg
     3601 gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg
     3661 accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt
     3721 tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc
     3781 gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc
     3841 gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc
     3901 atcggatatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg
     3961 gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac atcacccagc
     4021 atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac cgcaggaatc
     4081 gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact
     4141 gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc
     4201 gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata gccgttaccg
     4261 cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct ggtacgttct
     4321 ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg
     4381 aacaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt
     4441 aaggaacgcg cttcagccag cagttgctgc tcgcgcttta ggcgacgctt ctgattgaag
     4501 aactctacgc tcttactgaa gaagattgcc caggtgacta cggaggccaa aataagccca
     4561 atcatcacgc acttaacgac aatatcggcg tgctgataca taccccagac ggaaaggtct
     4621 gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca
     4681 taataatatc aaaacgacgt cgaattgata gtcattctca ttactatttg catactgccg
     4741 tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc
     4801 gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt
     4861 agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca
     4921 actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata gcgtgattca
     4981 gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg
     5041 cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca
     5101 agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc
     5161 ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac
     5221 tgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac
     5281 atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa
     5341 aatcgtgttt ctggaatcgc caggctccat caccatggaa gtccacgacg ttccggcgat
     5401 tgttgccgcc gtacgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc
     5461 agccggtgtg ctgtttaagg cactggattt tggcatcgat gtttctattc aagccgccac
     5521 caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca atgcccgttg
     5581 ctgggagcag ctacgggaaa acgcctatct gatgggccag atggtcgatg ccgataccgc
     5641 ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag
     5701 cagtctgaaa gtggctgaat ggctggcaga acatccgcaa gtagcgcgag ttaaccaccc
     5761 tgctctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag gtagcagcgg
     5821 gctgttttcc tttgtgctta agaaaaaact cagtaatgaa gagctggcga actatctgga
     5881 taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc
     5941 gaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac
     6001 cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc
     6061 cggttttgcg cgaattgtat aacattgcca cttttggaca attttgcaga catttaattg
     6121 tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgaatc aggagtacaa
     6181 taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata
     6241 tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg
     6301 ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat
     6361 ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccag ggggttatgg
     6421 attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt
     6481 atattcaggg gcgctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc
     6541 ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc
     6601 tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg
     6661 gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg tgggtcagcg
     6721 tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc
     6781 aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca
     6841 tgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ccgttcccgg
     6901 ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc
     6961 cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag
     7021 ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc
     7081 ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa
     7141 ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc gacgctcagg
     7201 ttttcggtgt atttattctc aatccgtttc agcacgcggc taatcagact gaagtgagtc
     7261 tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga
     7321 atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg
     7381 agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt aatcccgctg
     7441 gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac
     7501 tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt ggcataggtt
     7561 tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac
     7621 acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat gccaggctcg
     7681 tacatcaccg gtgtacgtgc gaaaggcgtc tcaccataca acaaacgcac atcgggcaac
     7741 agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc cagcaagcgg
     7801 caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca atttgattgt
     7861 gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat tggcagaaat
     7921 gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag atcgttctct
     7981 gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct gcacacccca
     8041 acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat tcctcacgat
     8101 gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt tctcgatcaa
     8161 gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga gccaaacccg
     8221 gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt gactttcctg
     8281 ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc agcggataac
     8341 tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc
     8401 gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc caacgcaggc
     8461 gcggtgatct cccgtaaaac cactggcgac aagcaggcgt tccattctgc ccatgttcag
     8521 ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg tcaggtggct
     8581 aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa accggttgat
     8641 gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctgatcga agatggtccg
     8701 aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg ggcggcgacg
     8761 caggcgctga acggtttgat tggcgctggc gtaccgcagg actgggcaac gcatatgctg
     8821 ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc tatcgtcctg
     8881 cctgcactgt ggaatgaaaa acgcgatacc aagcgcgcta agctgctgca atatgctgaa
     8941 cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc gattgccgca
     9001 acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta cggtctggac
     9061 ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac ccaactgggc
     9121 gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc ccgctaagct
     9181 ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc acaacacctc
     9241 accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat ccaaccgtta
     9301 ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg caagcaagta
     9361 atgaggaagt aatcatcgcc attcaaaaag cgttagaagt gggttatcgc tcgattgata
     9421 ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca
     9481 acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag cgcccccgcg
     9541 aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc tacttaatgc
     9601 actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg atcgaattgc
     9661 aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat cacctgcaac
     9721 gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt catccgctga
     9781 tgcaacaacg ccagctacac gcctggaacg cgacacacaa aatccagacc gaatcctgga
     9841 gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc gatctggcag
     9901 ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat agcggcctgg
     9961 tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat gtctgggatt
    10021 tccgtctcga caaagacgaa ctcggcgaaa ttgcaaaact cgatcagggc aagcgtctcg
    10081 gtcccgatcc tgaccagttc ggcggctaac atgcaaattc tcccggtggc ggtaatgttc
    10141 cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt gttactcttc
    10201 cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc tcttaaaaga
    10261 acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat gaaaataata
    10321 cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc ttcacagacc
    10381 gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag tacgccttat
    10441 ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc gcttaccctg
    10501 aacgacaatc ttacgctgcc gggcaatatt ggctggctgg attatgatcc ggtttgtgat
    10561 tgtcaggatt ttggcgatct ggtgctagaa agcgttgcga taacccaaac tgacgccgat
    10621 catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa gaccacgcag
    10681 acactgaaaa tggtggcgga aaatggtcgt tgggtcattg acgatattgt cagcaatcat
    10741 ggcagtgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc tttagcttcg
    10801 ttgcaaaaag aacagccgga agcctttgtt gccgaactct ttgaacatat tgctgattat
    10861 agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa tgccttctat
    10921 aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga acggcaattt
    10981 atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt tgatgaaatt
    11041 cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac gctgaccaat
    11101 ggcaacaacg aagagcaaga actggtttta cagcggcgcg aaggcaagtg ggaaatcgct
    11161 gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa aactgccgcc
    11221 agattaaagc aatgagctga attaaataac aattagccgg aacaataaat aaaagggaac
    11281 actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc ttgctcagtg
    11341 gctgcgtcaa tgagcaaaag gtcaatcagc tggcgagcaa tgtgcaaaca ttaaatgcca
    11401 aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat gctgccaaat
    11461 ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat tgcctgcgct
    11521 gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt tgccgggtta
    11581 taggctgttt gctaaaaaaa agccaaacat aaggacatga ttatgcgacg ctggcacacc
    11641 tgttttttta ttctgctgtt ctgtggcttc agcggaaacg tgttgtgcaa agaatcgatt
    11701 aatgaagcga tagaacgttt gtaccaacca ttgaccagta atgacgatat accaataacc
    11761 gatgaattca gtctcgattt gcgaagtaat atgagcgcta ctcctggcaa aatgacaccc
    11821 tggctgaata ataatctttt gtgtcgctgt gtcgatgacg ctttcgtttc tgcagaaata
    11881 atccaaagca cacacctaaa tggcggccga acgcatgtac tgttgcagct ggttatcaaa
    11941 caaaatcgca cagatatcca ccggttactg gggctaatgc tggttcgtga aaatgataat
    12001 catcgttggc taataga
//
LOCUS       DAJVTY010000127        11944 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVTY010000127 DAJVTY010000000
VERSION     DAJVTY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11944
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.127"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            30..116
                     /locus_tag="OQQ66_003072"
     tRNA            30..116
                     /locus_tag="OQQ66_003072"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Leu,seq:cag)
     gene            complement(155..391)
                     /locus_tag="OQQ66_003073"
     CDS             complement(155..391)
                     /locus_tag="OQQ66_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1905915.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            532..1320
                     /gene="fhuF"
                     /locus_tag="OQQ66_003074"
     CDS             532..1320
                     /gene="fhuF"
                     /locus_tag="OQQ66_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1905916.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1358..2034)
                     /gene="bglJ"
                     /locus_tag="OQQ66_003075"
                     /pseudo
     CDS             complement(1358..2034)
                     /gene="bglJ"
                     /locus_tag="OQQ66_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(1992..2717)
                     /locus_tag="OQQ66_003076"
     CDS             complement(1992..2717)
                     /locus_tag="OQQ66_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1905917.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDF
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3336..4106
                     /locus_tag="OQQ66_003077"
     CDS             3336..4106
                     /locus_tag="OQQ66_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1905918.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            4097..4570
                     /locus_tag="OQQ66_003078"
     CDS             4097..4570
                     /locus_tag="OQQ66_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1905919.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4678..5217
                     /gene="dnaT"
                     /locus_tag="OQQ66_003079"
     CDS             4678..5217
                     /gene="dnaT"
                     /locus_tag="OQQ66_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1905920.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5220..5957
                     /gene="dnaC"
                     /locus_tag="OQQ66_003080"
     CDS             5220..5957
                     /gene="dnaC"
                     /locus_tag="OQQ66_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1905921.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            6006..6500
                     /locus_tag="OQQ66_003081"
     CDS             6006..6500
                     /locus_tag="OQQ66_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1905922.1"
                     /translation="MMETVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6754..9045
                     /gene="opgB"
                     /locus_tag="OQQ66_003082"
     CDS             6754..9045
                     /gene="opgB"
                     /locus_tag="OQQ66_003082"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1905923.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9184..10206)
                     /gene="lgoD"
                     /locus_tag="OQQ66_003083"
     CDS             complement(9184..10206)
                     /gene="lgoD"
                     /locus_tag="OQQ66_003083"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS1905924.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10345..11259
                     /locus_tag="OQQ66_003084"
     CDS             10345..11259
                     /locus_tag="OQQ66_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1905925.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11474..>11944
                     /locus_tag="OQQ66_003085"
     CDS             11474..>11944
                     /locus_tag="OQQ66_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1905926.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         3100 a         3042 c         2800 g         3002 t
ORIGIN      
        1 gcgatatcaa aaaaaagtaa gatgattgtg cgaaggtggc ggaattggta gacgcgctag
       61 cttcaggtgt tagtgtcctt acggacgtgg gggttcaagt cccccccctc gcaccaatta
      121 tctttacttc ctttcttgtt tcttccttga tttttcatcc ctgattcaaa ttcattatca
      181 tcagcatgat ggcagcaata agcgccaggc acgatggtag caaaacgtaa tgccgtaact
      241 gtttgtgata cagcatcacc ggcgtcaata ctaatcccat cagaatgaca atccgccact
      301 gatccggcga taaatccgca tacatcagcc ctgcaatcag caatcccggc agcaacactc
      361 cccagccact gcccagcgta cgttgcaaca tggtttcatc tctttcattg ataatgataa
      421 ctaatatcat atgataattt ttatcatttg caagccagag aaatcccttg ctatcgggta
      481 aacctatcgc taggattagc aatcattatc atttagatta ctatcccgat tatggcctat
      541 cgttccgcac cgctctatga agatatcatc tggcgaacgc atctccagcc gcaggatgct
      601 gggcttgcac aagccgtacg tgcgacgatc gccaaacatc gtgaacattt gctcgagttt
      661 atccgcctgg atgaacccgc tccgcttaac gccatgacgc tggcgcaatg gtcatcaccg
      721 aatgcgctaa gttctctact cgcggtctat tccgatcata tctatcgcaa ccaaccaacg
      781 atgatccgcg agaacaaacc gctgatctca ctatgggcac aatggtatat cggcctgatg
      841 gtgccaccat taatgctggc gctactgacg caggaaaagg cattagatgt gtcgccggaa
      901 catttccatg ctgagtttca cgaaaccgga cgcgtcgcct gtttctgggt cgatgtgtgt
      961 gaagataaaa acgcaacacc acattcgccg cagcagcgaa tggaaacgtt aatcagccag
     1021 gctctggttc cggttgtgca ggcactagaa gcgaccggag agatcaacgg caaacttatc
     1081 tggagtaata ccggttattt gatcaactgg tatctcactg agatgaagca actgctcggc
     1141 gaggcgacgg ttgaatcgct gcgccatgcc ctcttttttg agaaaacgct cacgaacggt
     1201 gaagataatc cactctggcg taccgtcgtg ctgcgcgatg gcctgctggt gcgccgaacc
     1261 tgctgccagc gttatcgctt accggatgtg cagcaatgtg gcgattgtac gctgaaatga
     1321 aagcactgcc ggggaagtaa acccggcatc atgcggatta atagggatgc aacacattac
     1381 ttgcttcgca atgccgcata cataacagaa tatctgcggc ctccaacacc cctgcatcag
     1441 aactgacgcc cagtttcgac atcacattaa atttatgtgc acggatcgtt ttgatattgc
     1501 gtttaagctg ctcggcaatt tgtgtcattg agtagccacg cgacataaag cgcaatattt
     1561 ctctctccgt cgggcttagc gtccggcttt ggttaatgta ccactgattg ttcagtcggt
     1621 cggtcgcctg acgtacacca tttaatgaca aaaagagttc ctgatgaaaa atctccagcg
     1681 ttgacgcttt acttaataca ccgtccagcg gtgatggcga caatgaacca atcagccgag
     1741 cttcaatatc atcatccgca ataactaaac gccgggtacg cggaaactta atcgccagtt
     1801 cagtcaggca agataatccc tctctgcgct cacttctcat ggcagaaaaa gaaaaatgac
     1861 cgccgcaaat gagatccgcg acatggcatc ctgaaatgac tcctgactgg tataggtatg
     1921 gagcttataa gggttacccg caaactttct gaataaactc tcaataccga tactgctcat
     1981 gacgcatttt tctatgagtg cgacatttct cttcttaatt cggctgtgtt ccattctgcg
     2041 gcatctccat aaacagaagg caagttaagt tctcttgcac cctgcgcgct atttatccat
     2101 gcatacattt cagcgttgct gtgaattgcc agtcgacgca tcgcactgtt tttttgggcg
     2161 ctgatagttt tattactttt cttaagcaac gaaactatct ggttgatgcc ccatccttta
     2221 cctaatagcc gcagtacctt gcgctctgaa agagtgagaa tgacggagcg atagctaaaa
     2281 tcatgagttt cattcattgc cggagaggtc agcatggcgc tgatacgctc tgcgtgcgta
     2341 ttgccggaac gtacggtctt gaccagattc tcaatgggtt caacatcaga caataacgtc
     2401 gccgtagggc acatgagcaa ttctactgcc tgggaatacc aggagcgtga caccatgaaa
     2461 acccagtgaa tttcccgata ttgtgagatt aaagaataat aatgttcaca aacagaacgt
     2521 ggatcttcac tttcaccggc taaatcagca atgactaatc cggaacgacg taattgtaat
     2581 aaggtaaagt cctccgcaga ggcgctggat attatttcat attcagggaa gtgagtcctc
     2641 atgacctctt ttaaccctgc ttgcataaca ggtattttac tgataacaat tccatttttg
     2701 cagcatcctg gcaacatatt tcctccacat ccactgtcta aaaatcatga tggtttattc
     2761 aggatattca ttccttaaat gtttccagca aattaataga ttaagcgaat aaattatcct
     2821 tagcattcac tttcatatca gcatactcct gctgcacaac aactccactg catgtgtcag
     2881 aaggttagca tcaaatgata acctggtgaa atagcctgga gacatttaaa atatctacat
     2941 taagttgtta ggaatactta atgaaaaaca gaaatcacat tcatttaatt tataattaaa
     3001 ttatatccct tcccgcagaa tgaaaagtca cacaaccaat atttattcaa tgaaatctga
     3061 tggttttaac ttgttaacat taaaaaacgt gcccggttat ctattatcct gattcgtaaa
     3121 ggccaaacaa taaattcatt atagtgataa tattcatgtg atagtttgtt tgcggcgaga
     3181 gataattcgc tttttatcac cgagcttgtc ccgataacca ggattaatca gcgcaatttt
     3241 gatcttaacc taccgcaaaa caaccgtgct tcatgataca aatggattct cattatctga
     3301 acaatacgca gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg
     3361 taacacggct ttgtatccag tgtggattat ttcttttgca acatggtgcg gaaagcgcgt
     3421 tggttgatga gctttcctca cgactgggtc gggcgttggg gatggacagc gtcgaaagtt
     3481 ctatctcttc gaacgccata gtgctgacaa ccattaaaga tgggcaatgc ctgacatcga
     3541 cacgtaaaaa tcacgatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg
     3601 tgattctggc ggagcaccat ctgctggatt acaaaggcgt agagaaacga tttagccaaa
     3661 ttcagccatt acgttaccca agatggctgg tagccttaat ggttggcctt tcttgcgcct
     3721 gtttctgcaa actcaataaa ggtggctggg atggtgccat catcaccttc tttgccagta
     3781 cggccgcgat gtatatccgc cagctgctgg cacaacgtca tcttcatccg cagatcaact
     3841 tttgcctcac cgctttcgcc gccaccacca tttccggatt gcttttgcaa cttcccactt
     3901 tcagcaatac acccactatc gcgatggccg ccagcgttct gctgctggtg ccgggatttc
     3961 cgttgattaa tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct
     4021 gggcgatcgc cagtctgctg acactggcta cctgcgtcgg cgtggtgatg gcactgacga
     4081 tttgggggct acgcggatgg gtgtgatcga gtttctgttt gcattggcgc aggatatgat
     4141 cctcgccgcc attccagcgg tcggctttgc gatggtgttc aacgttcccg ttcgggcatt
     4201 acgctggtgt gcgctgcttg gcgcgattgg tcatggttca cgaatgatct taatgaccag
     4261 agggctgaat attgagtggt caacctttat ggcctccatg ctggtcggca ccattggtat
     4321 tcaatggtcg cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ctgttatccc
     4381 tatgttcccg ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa tcagccagtt
     4441 aggttacagc gaaccgttga tgattaccct gttaaccaac tttcttacag cttcatcgat
     4501 tgttggtgcg ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc
     4561 tcgcgtataa aattgcctcg cttaaagcac acggatgaga gacagcctcc tctcctccgt
     4621 gtgttactat aaaagttatc tcccttctcg ttcatcgttc catatttgag aaacagtatg
     4681 tcttccagag ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa
     4741 accgttctgg caaaagctga aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg
     4801 ttttatgccg tcacgcctgc acgcctggct gaactgctgg cgctggaaga aaagctggcg
     4861 cgtccgggaa gcgatgtcgc tctggacgat caactctatc aggaaccgca agccgctccc
     4921 gttgctgtac ccatggggaa attcgccatg tatccggact ggcaacccga tgccgatttt
     4981 atccgcctgg cggcgctatg gggtgtggca ctaagagagc cggtgaccac cgaagaactg
     5041 gcctcattca ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt gcagtggcaa
     5101 caaaaactgg cgcgcagcct gcaaatcggt cgtgccagca acggcggact gccgaaacga
     5161 gatgtgaata cggtcagcga acctgacagt caaattccac caggattcag agggtaacga
     5221 tgaaaaacgt tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac
     5281 ctgcattcaa aacgggtgaa gaactactgg cctggcagaa agaacaagga gcgatccgct
     5341 ccgccgctct cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg
     5401 gtattcggcc actgcatcag aactgctcct ttgagaacta tcgcgttgag tgtgaagggc
     5461 agatgaatgc gttaagcaaa gcgcgccagt acgtcgaaga gtttgacggc aacatcgcca
     5521 gcttcatctt ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg gcaatctgca
     5581 acgagctgct gctacgcggt aaatccgtat tgatcatcac cgtggccgat attatgtcgg
     5641 cgatgaaaga taccttcagg aatagcggta ccagtgaaga acaactgctt aacgatctga
     5701 gcaacgttga tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa
     5761 aagtgatcat caaccagatc gtcgatcgcc gctcttcttc caaacgccca accgggatgc
     5821 tgaccaacag taatatggaa gagatgacca agttgctggg cgaacgcgtg atggaccgta
     5881 tgcgcctggg taacagtttg tgggtgatct tcaactggga tagctaccgt agccgggtaa
     5941 caggtaaaga gtattaatcc ccgtttctgg cggtatactg cgacacaatt ccaccttgag
     6001 taatgatgat ggaaactgtt aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa
     6061 tttccaccgg ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac
     6121 ttggcaacca aaacagcacg acacaggaag gcggttggtc gctcgcgtca ttaactaact
     6181 tgcttagcag cgggaaccag gccttaagcg cagataacat gaacaacgcc gcaggcattc
     6241 tgcaatactg cgcgaagcaa aagctggcgt cggtaaccga tgccgaaaac atcaagaacc
     6301 aggtgctgga aaagctgggc ctgaacagtg aagagcaaaa agaagacacc aactatctgg
     6361 atggcattca gggtttgctg aaaaccaaag atggtcagca actcaatctg gataacatcg
     6421 gaacgactcc gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg
     6481 ggctgaactt catttcctga accgcgaaac gccgcgtttt agctgttacc cggctgacgc
     6541 ggctctctcc tgcccttttc cctgcttctg gacggttttc cccctgttac acgggaagcg
     6601 accgcgatca aaaaagtctg tttataaact aattcctaac aacatcgcat ttttttgaca
     6661 ctagaattgc agcaatatgg cgacgccatt acattgttct tctcaaaaag cttaaacgtt
     6721 ggctaaacgg cccggtttag tgaggatcgc ccgttgtcag aactactctc tttcgccctt
     6781 tttctcgcct ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg gtggtttgca
     6841 gccacattaa cggtgctggg gctatttgtc gttttaaata tcaccctgtt tgccagcgac
     6901 tattttactg gcgatggtat taacgacgcg gttctctata ccttaaccaa cagcctgacc
     6961 ggtgctggcg tcagcaaata catcctgccg ggtatcggca ttgtgctggg gctgacagcg
     7021 gtgttcggtg cgctgggctg gatcctgcgc catcgtcgcc atcatccgca ccattttggt
     7081 tacagcctgc tggcgctctt actggcgctg ggttcggtgg acgccagccc ggcatttcgt
     7141 cagataacgg aactggtgaa atcccagtca cgcgatggcg acccggactt tgcggcttat
     7201 tataaagagc cgtcgaaaac tatccctgac ccgaaactca acctggttta tatctacggc
     7261 gaaagtctcg agcggaccta ttttgataac gaagctttcc cggatctcac gcctgaactg
     7321 ggcgcgttga aaaatgaagg cctggatttc agccacacgc agcagctgcc aggaacggat
     7381 tacacgattg cgggcatggt ggcttctcag tgcggcatac cgctgtttgc cccctttgaa
     7441 ggcaacgcct ccgcctctgt ctccagcttc ttcccgcaga acatctgtct gggcgatatc
     7501 ctgaaaaact cgggttatca gaactatttc gtgcagggcg cgaatctgcg ttttgccggt
     7561 aaagatgtgt tcctgaagtc gcatggcttc gaccacttat acggctcaga agagctgaaa
     7621 agcgtggtgg ccgacccgca ctatcgcaac gactggggat tctacgacga taccgttctc
     7681 gatgaagcgt ggaaaaagtt tgaagagctt tcccgctcag gtcagcgatt ctcactgttt
     7741 accctgacag tcgataccca tcacccggat ggttttatct cgcgtacctg taaccgcaaa
     7801 aaatatgatt ttgacggtaa gccgaaccag tcattcagcg cggtaagttg cagccaggag
     7861 aatatcgcgg cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga taccgtcatc
     7921 gtcgtctctt ctgaccattt agcgatgaac aacacggcgt ggaaatacct caataagcag
     7981 gatcgcaata acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga gacgctggca
     8041 gtgaagcgta acacgatgga taacggcgcg acggtgctgg acattctcgg tggcgataac
     8101 tatctcggac ttggtcgtag cagtttatcc gggcagtcga tgtcggaaat cttcctcaat
     8161 atcaaagaga aaacattggc gtggaagccg gatatcatcc gcctgtggaa attccctaaa
     8221 gagatgaaag agttcaccat cgaccagcag aaaaacatga ttgccttctc gggtagccat
     8281 ttccgtttgc cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct gccggaaagc
     8341 gaatactcag caccgctgcg tttccagctg gccgatttcg ctccacgcga caatttcgtc
     8401 tgggttgacc gttgctataa gatggcacaa ctctgggctc cggaactggc actctccacc
     8461 gactggtgtg tctcgcaagg gcagcttggc ggtcagcaaa ttgttcaaca tgttgacaaa
     8521 acaacatggc agggcaaaac ggcatttaaa gatacggtca tcgacatggc gcgttataaa
     8581 ggcaatgtcg atacgctgaa aattgttgat aacgatattc gctacaaagc cgacagtttc
     8641 atcttcaacg tcgccggtgc gccagaagag gtgaaacagt ttagtgggat ttcccgtcca
     8701 gagtcgtggg gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat cgagtacaag
     8761 catccgctgc caaagaaatt tgacctggtg attaccgcca aagcatacgg caataacgcc
     8821 agccgtccta ttccggtacg cgtaggcaat gaagaacaaa cccttgtgct gggcaatgaa
     8881 gtgaccacca ccacactgca tttcgataac ccaaccgatg ccgacacact ggtaattgtg
     8941 ccgccggaac ctgtctcaac caacgaaggg aatatcctcg gacactcgcc acgtaagctc
     9001 gggatcggca tggtggaaat taaagtggtg gaacgtgaag gataattcct gactgatgtg
     9061 taggtcgcat aaggtgcgac ctacacacct tctgcggcct acaaaacatc attgagaaac
     9121 agaaaaaaac agcgcccaac acttccccgg acgctgcatg gcaactttaa taatctcgcc
     9181 ttatcagaaa gtaattacac ccttaatcaa ctcacgattg ttaatcacat cgcgctcgta
     9241 ggtttctgcc agcgtggcga acggatagcg atgggttaac atcatgtcag cagtgatttt
     9301 tccttccgcc atcagtcgac cgactttagc aaaatcttcc ggcgtggcgt tgcggctacc
     9361 catcatcgtc gtttcttttt tatggaattc cggatcggag aactgcaact cacctttaaa
     9421 caggccgaca aataccaccg tgccgccgtg acgaatcaaa ttcacggtgt tattcatcgc
     9481 atgttgatta cctgtcgcgt cgatcacttt ctgcgccagc gaaccaccaa actgcgcccg
     9541 tagctgcgcg tcaaaatctt cggctgacgg gtccagtaaa ggtaattcca gacgcgttgc
     9601 cacatgttca cggcgcgccg gactggtatc cgccaccacc acctgtgcgc catcggcttt
     9661 agcgattgcc gccgcgccca gaccgattgg ccccgccccg accaccagca cctgctcgcc
     9721 gggagcaatg gctgcgcgac gcaccgcatg agcgctaata gcgaaaggtt caatcaatgc
     9781 tgccgcctgc gggtcaatac cgtctgcggg caaaatgttc gccaccggca cgctcaaata
     9841 ctcactaaaa ccgccatcct gatgcacgcc aatgactgaa attttttcac agcaattggt
     9901 acgcccgctt ttacacgccg ggcattgctg acaggcaaca taagggatca cagcaacttg
     9961 cagaccattt ttaagatcag caatattttt acccagccta acaatctccc cacatatttc
    10021 atggcctaaa acacgtggat aactaaaaaa tggttgattt ccaccccagg catgaatatc
    10081 ggtaccgcaa atcccgacag acttaatttt tattaatgct tcattgtcac ccggaatagg
    10141 tatctcgcgt tgtttccaga ctaattcttt cggctgctgg caaattaaaa cattcatcgt
    10201 agacatgatt cggctctcca ttttttgttg ccttagatat caatgaaaat gaaaaatggc
    10261 agcggaacgg aaaatctgtt ttgtgaaaac acacgcataa aaatgtttta aatcgggttt
    10321 taatggactc acaaggagga aaatatgagt cgttcacaaa atttacgcca caatgtgatt
    10381 aaccaggtca ttgatgatat ggcgcggggt catatccctt ccccacttcc atcgcaaagt
    10441 gcgctggcgg aaatgtacaa catcagccgc accacagtgc gtcatattct cagccattta
    10501 cgcgaatgcg gcgtcttgac gcaggttgga aacgactatg ttattgcgcg taaacctgac
    10561 cacgatgacg gtttcgcctg tactaccgct tcaatgagtg aacaaaacaa agtgtttgag
    10621 caggcttttt tcacgctgat aaaccagcgc cagttacgcc caggggaaac tttttctgaa
    10681 ctgcaactgg ctcgggccgc gggtgtcagc ccggtggttg taagagaata tcttttaaaa
    10741 ttcggccgtt ataatcttat tcaaagcgaa aagcgcggcc agtggagcat gaagcaattc
    10801 gatcaatcct acgccgagca gctgtttgag ctacgcgaaa tgctcgaaac acattcactt
    10861 cagcactttc tcaacctgcc tgatcatgat ccccgctggc tgcaggcgaa aaccatgctc
    10921 gaacgtcatc ggctactgcg tgacaacatc ggcaatagct ttcgcatgtt ctcgcaactt
    10981 gacagagatt ttcactcctt actgctttca gctgccgaca atatcttttt tgatcaatcg
    11041 cttgagatca tctcagtcat ctttcatttt cactatcaat gggacgaaag cgatcttaaa
    11101 cagcgcaata tcattgccgt cgatgagcat atgaccattc tcagcgctct gatttgccgc
    11161 agtgacctcg atgccaccct tgcgctccgt aaccatttga attcagctaa acaatcaatg
    11221 attcgatcca ttaatgagaa cacgcgttat gctcattaag tacggattaa aaacagcaaa
    11281 ctattagcgg gtgcacggtt ttatcaaaag ataaataccg ggaaattccc gcttacctat
    11341 gctcacaatc agtcgactaa aaatgttttt gtgagcggta gtaaagtcct aaaactttaa
    11401 cctgccgtta atgtcaacct tgcgtaatgc acaattacgt aaacaattaa aattataaat
    11461 acaggagtcc ggcgtggaaa aagaaaatat caccatcgat ccgcgttctt catttactcc
    11521 atcttcgtcg gcagatattc ccgtgccacc agatggatta gttcaacgca gtacccgaat
    11581 taaacgcatt caaaccaccg ccatgttgtt attatttttt gcggcggtaa tcaattatct
    11641 cgaccgcagt tcgctgtcgg tagcaaattt aacgattcgt gaagaattgg gattaagtgc
    11701 cactgaaatc ggcgctttgc tctccgtgtt ttcactcgct tacgggattg cgcaacttcc
    11761 ttgcggccca ctattggatc gtaaaggccc acgcctgatg ctgggactgg ggatgttctt
    11821 ctggtcactg ttccaggcaa tgtctggcat ggtgcacaac tttacgcagt tcgtgttggt
    11881 gcgtatcggt atggggattg gtgaagcgcc gatgaaccca tgcggtgtaa aagtcattaa
    11941 cgac
//
LOCUS       DAJVTY010000128        11873 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVTY010000128 DAJVTY010000000
VERSION     DAJVTY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.128"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(39..353)
                     /locus_tag="OQQ66_003086"
                     /pseudo
     CDS             complement(39..353)
                     /locus_tag="OQQ66_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(468..866)
                     /locus_tag="OQQ66_003087"
     CDS             complement(468..866)
                     /locus_tag="OQQ66_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCS1905927.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OQQ66_003088"
     CDS             complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OQQ66_003088"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1905928.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OQQ66_003089"
     CDS             complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OQQ66_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1905929.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OQQ66_003090"
     CDS             complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OQQ66_003090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1905930.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OQQ66_003091"
     CDS             complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OQQ66_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1905931.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7427..7507
                     /locus_tag="OQQ66_003092"
     CDS             7427..7507
                     /locus_tag="OQQ66_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905932.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7737..7877
                     /gene="rpmH"
                     /locus_tag="OQQ66_003093"
     CDS             7737..7877
                     /gene="rpmH"
                     /locus_tag="OQQ66_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1905933.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7894..8253
                     /gene="rnpA"
                     /locus_tag="OQQ66_003094"
     CDS             7894..8253
                     /gene="rnpA"
                     /locus_tag="OQQ66_003094"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1905934.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8217..8474
                     /gene="yidD"
                     /locus_tag="OQQ66_003095"
     CDS             8217..8474
                     /gene="yidD"
                     /locus_tag="OQQ66_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1905935.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8477..10123
                     /gene="yidC"
                     /locus_tag="OQQ66_003096"
     CDS             8477..10123
                     /gene="yidC"
                     /locus_tag="OQQ66_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1905936.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10229..11593
                     /gene="mnmE"
                     /locus_tag="OQQ66_003097"
     CDS             10229..11593
                     /gene="mnmE"
                     /locus_tag="OQQ66_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1905937.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11836..>11873
                     /locus_tag="OQQ66_003098"
     CDS             11836..>11873
                     /locus_tag="OQQ66_003098"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCS1905938.1"
                     /translation="MNILHICVTSKW"
BASE COUNT         2713 a         3251 c         3069 g         2840 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatacgtg aaccgacttc gcgagagagt
       61 ttttccagga aacgatagtc gtcataactg agagcagttt gcgccacggt gctaccatca
      121 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
      181 tgcagctctt tcaggtagtt gtgaacgcct gcataagggc gacccgtcgt tagcacgaca
      241 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
      301 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
      361 ccaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
      421 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
      481 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tgaagacact
      541 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gttctgccag taaactggaa
      601 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgggc
      661 gccgattcca gttgctcgcc gctaactgat tgattgctct gctgattact caaccaggtt
      721 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
      781 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
      841 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
      901 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
      961 tctccgccct ttttttatag cttcttgccg gatgcggcgt taacgtctta cctggcctac
     1021 aaaatcatta aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
     1081 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
     1141 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaacaggt
     1201 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg
     1261 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
     1321 gaccgcgacg agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
     1381 gacgacgctc gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac
     1441 ccagcgtgca gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt
     1501 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
     1561 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
     1621 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
     1681 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
     1741 tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg
     1801 ccggtgcgct ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
     1861 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
     1921 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
     1981 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
     2041 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
     2101 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
     2161 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
     2221 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
     2281 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
     2341 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
     2401 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
     2461 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
     2521 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
     2581 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
     2641 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
     2701 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca
     2761 cttcgacgcc aataccgtct ttttcggtgg agaagtagaa gatattcgga tggatcggcg
     2821 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
     2881 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
     2941 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
     3001 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
     3061 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
     3121 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
     3181 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
     3241 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
     3301 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
     3361 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
     3421 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
     3481 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
     3541 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
     3601 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
     3661 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
     3721 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
     3781 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
     3841 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
     3901 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
     3961 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca
     4021 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccac cagcgggatc
     4081 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
     4141 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
     4201 aagcatgccc agtcgagaaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
     4261 ccttctggcg ttatcagctg catcggcatc aggtgcgcca gctccgcgac tttatgcccg
     4321 tcagtaccgt cgatgcggac tttgctgtcg ccttgtttgt ctttggttaa gccaatcgcc
     4381 gtctcgcgct cttcgccctg taatcgccca tggagaacaa atgcctcctg ctcatggcga
     4441 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
     4501 tccagcacac tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
     4561 aaggcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
     4621 attacagtct cattggcatg acaacataag ccgcgctctg gctggccgca tcttcaatct
     4681 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
     4741 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
     4801 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtgatt ttcagctggt
     4861 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatt gccgcacggg
     4921 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggta
     4981 gaacgcggcg gtaatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
     5041 caacgtgggc acgaatgtta ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt
     5101 cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca
     5161 aagattgacc aattggcatt gaacagactg ccagacggtg accatcggtc gctacggtgc
     5221 gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
     5281 gcgccatcga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
     5341 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
     5401 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
     5461 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
     5521 ttgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
     5581 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
     5641 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
     5701 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
     5761 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
     5821 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
     5881 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
     5941 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
     6001 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
     6061 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
     6121 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
     6181 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
     6241 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
     6301 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
     6361 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
     6421 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
     6481 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
     6541 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta
     6601 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
     6661 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
     6721 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
     6781 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
     6841 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
     6901 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
     6961 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
     7021 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
     7081 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
     7141 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
     7201 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
     7261 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
     7321 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
     7381 cctgccgcga ggcgggcacg atttacgccg catattgaaa aatttaatga ccatagacaa
     7441 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
     7501 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
     7561 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
     7621 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
     7681 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
     7741 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
     7801 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
     7861 tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag
     7921 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
     7981 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
     8041 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
     8101 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
     8161 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
     8221 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
     8281 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
     8341 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
     8401 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
     8461 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
     8521 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
     8581 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
     8641 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
     8701 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
     8761 gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
     8821 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
     8881 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
     8941 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
     9001 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca
     9061 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
     9121 gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt
     9181 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat
     9241 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
     9301 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
     9361 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
     9421 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
     9481 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
     9541 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaaac
     9601 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
     9661 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
     9721 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
     9781 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
     9841 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
     9901 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
     9961 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    10021 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    10081 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    10141 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    10201 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    10261 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    10321 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    10381 taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa
    10441 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    10501 cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    10561 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    10621 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    10681 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    10741 agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc
    10801 ccagctcaat gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag
    10861 tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag
    10921 cctgttaaac gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac
    10981 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    11041 cgccgggcta cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca
    11101 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    11161 ggatccggca gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac
    11221 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    11281 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    11341 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    11401 ccacctacag gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt
    11461 gggagcctgg gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag
    11521 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    11581 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    11641 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    11701 ttataatctt aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    11761 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
    11821 aggaataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gtt
//
LOCUS       DAJVTY010000129        11708 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVTY010000129 DAJVTY010000000
VERSION     DAJVTY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.129"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            458..1114
                     /gene="pphA"
                     /locus_tag="OQQ66_003099"
     CDS             458..1114
                     /gene="pphA"
                     /locus_tag="OQQ66_003099"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCS1905939.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1115..1306)
                     /locus_tag="OQQ66_003100"
     CDS             complement(1115..1306)
                     /locus_tag="OQQ66_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1905940.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1411..1647)
                     /locus_tag="OQQ66_003101"
     CDS             complement(1411..1647)
                     /locus_tag="OQQ66_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1905941.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1765..3204)
                     /gene="rsmF"
                     /locus_tag="OQQ66_003102"
     CDS             complement(1765..3204)
                     /gene="rsmF"
                     /locus_tag="OQQ66_003102"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1905942.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVDIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3284..5917)
                     /gene="yebT"
                     /locus_tag="OQQ66_003103"
     CDS             complement(3284..5917)
                     /gene="yebT"
                     /locus_tag="OQQ66_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS1905943.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5886..7169)
                     /gene="yebS"
                     /locus_tag="OQQ66_003104"
     CDS             complement(5886..7169)
                     /gene="yebS"
                     /locus_tag="OQQ66_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1905944.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7299..7796
                     /gene="msrC"
                     /locus_tag="OQQ66_003105"
     CDS             7299..7796
                     /gene="msrC"
                     /locus_tag="OQQ66_003105"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1905945.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7893..8591
                     /gene="proQ"
                     /locus_tag="OQQ66_003106"
     CDS             7893..8591
                     /gene="proQ"
                     /locus_tag="OQQ66_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1905946.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8611..10659
                     /gene="prc"
                     /locus_tag="OQQ66_003107"
     CDS             8611..10659
                     /gene="prc"
                     /locus_tag="OQQ66_003107"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1905947.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10851..>11708
                     /gene="htpX"
                     /locus_tag="OQQ66_003108"
     CDS             10851..>11708
                     /gene="htpX"
                     /locus_tag="OQQ66_003108"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1905948.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEAL"
BASE COUNT         2999 a         2888 c         3026 g         2795 t
ORIGIN      
        1 gcacgtcgta tgcaagaacg tgccacggct ggctgatgga tgttcgatag cgcgagtttg
       61 aatgaaaatc agccggagat gattttacat aattgctacg gaattattca atacaggaat
      121 tgcttgcgtc tgcatggatt gacctgaaat tttcccgaaa atttctctaa aaaactcgaa
      181 aaaaatggta actggttgaa tgtattaata tgcaatggta cgtgtcaggg attaaaagat
      241 gaacgtaaat ttattcaacg cattaatttt aaagggtttt attgtttgtt gacgaaaaca
      301 ggaatcgtgt tcggtctctt tttatctgtt aaaagccaga agcatttcct tcgctgactt
      361 tatagtcaac cataacacac actctactgt ctgagtccag cgttttttaa cattcttgtt
      421 aagattatgt gatctttagc acgggaggaa aatattgatg aaacagcctg cgcccgttta
      481 tcagagaatt gcgggtcatc aatggcgaca tatctggctt tctggcgata tacacggttg
      541 tcttgagcag ttgcgccgca aattatggca ttgtcgtttt gatccgtggc gagatttact
      601 tatctcagtg ggagacgtta tcgatcgtgg gccgcaaagt ttacgttgtc tgcagttact
      661 ggaacaacat tgggtttgtg cggtaagagg caatcatgaa cagatggcga tggatgcgct
      721 ggcatcccag cagatgtctt tgtggttgat gaatggcggc gactggttta ttgcgctggc
      781 agataatcaa cagaaacaag cgaaaacggc gctggaaaaa tgtcagcatt tgccctttat
      841 tcttgaagta cacagtcgca ccggcaagca tgttattgct catgcggatt atccagatga
      901 tgtttatgaa tggcaaaagg acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt
      961 aggtgaacgc caaaaagggc agggaattac aggtgctgat catttctggt ttggtcatac
     1021 accgttgcga catcgcgtgg atattggcaa cctgcattat attgataccg gtgctgtctt
     1081 tgggggcgaa ctgactcttg tgcaattgca atgattaaaa atcaccgtac tcctgtgcag
     1141 gtcgccagaa accatctata aaatcctcaa tcggaaaaca accgccctgg cggatccgtt
     1201 gatcgctcat agaataaaga cactgctgtt ccgtgttgta gacatccaca acaatatctt
     1261 cacaaccgcc atccaggtag caaacaaaaa gtaccagcgc gaacatttca tccccgaagt
     1321 gtggtgccgt accgttaagt ttaggagaga ttttacaacg ggggaataac caggacaaat
     1381 aacccgccat gccgacgggt tctttttgga tcaggcaaga cgcataatcc aggcatcaga
     1441 ttgttgatcg taacgggtac ggtaaaggac agagtgttcg ccatttagca gggcagggat
     1501 actggtttca gcatcccagg catcgagttg catacataaa tcggggtcag atttacaagg
     1561 aatggttaac gttcgatcac ccggcgattc accgtgtaat tcgccgtcgt cgatttcaaa
     1621 agcgcctatg cgcacactgg ttttcatctt cgtgctctcc atttagctgc cggttgtggt
     1681 acgaccagtc cgatcgcacc atttttcagc ctagttgagg cagccgaatg tgccagtcaa
     1741 aaagtgcgct ttttttgtgc gctgtcaggc gttaccggta aaaagtttcc catcgcgcac
     1801 cagttcacgc ggatagctgt ttttcaatcg cgaaccaatc cgtttggcta aaccaatcgg
     1861 ttgatgctgg aaagtaacca acacgtcatc cgccactggc gcggcttgcg ggtaaacatc
     1921 gcgcccgcga taccactcct ccgcttcctg cggtgtcagt tcaaaagcgt tcacattgtc
     1981 gggggtggca agggcaataa ctgcttcatg ctgccagcga taacctttgt tgtgcgtttc
     2041 ggcaagttta atccccaagc gagaaaatcg gactttaccg atcagggctt caatgtccac
     2101 cgggaacaac cacagttctt tgtcacgctg ccagaggcgc aggttttcat cccagtttaa
     2161 gccaacacct gcagccgcct ggcgaatttg tccagcttcg cgatctttca ccgggctgaa
     2221 cggaaaatta ccgactttgt atttgggggc gggtaaggcg ggaatcgcct gagttttacg
     2281 cagacgagca acgaagaagc cttcgcagtc gtaaatttgt gggaaaacat gcaaaaagcc
     2341 ttcttcggtc agcgctttgt ttgcaccagg gaagagatcg ccaagtggta aaaactctac
     2401 tgcgtcgggg taagtctctt tcagccacag gcaaacggct tcgttttctt cctggtttaa
     2461 ggtacaggtc gagtaaacca gcgtaccacc aggacgtaat gcatgaaagg cgctgtcgat
     2521 aagctcccgt tgtgtagctg cgatttcctg attgctttct ggtgaccagt tttttagcgc
     2581 atcgggatct ttacgcacca cgccttcgcc ggagcaggga gcgtccagca aaatggcatc
     2641 aaacatttct ggcactgccg caccaaacac gcggccatca aaatgtgtga gcgcaacatt
     2701 actgatgcca cagcggctga tattggcatg taacactttt acccgactgg cggaaaactc
     2761 attggcaagg attgcccctt cgttattcat ccgcgcggca atttgcgtcg tttttgagcc
     2821 tggcgcagca gcgacatcca tcacccgctg tggtgcatta ccgtcagcaa acaaggcggc
     2881 gacgggcaac attgaactgg cttcctgaat ataaaacagg ccacttaaat gctcggcggt
     2941 actacccaat ggcaatgcat cttcattgtc gcgttcaatc cagaaacctt cttcacacca
     3001 cggaattggc gtaagcgtcc agccataagg agcggttaat tgcaggaaat cagcaacgga
     3061 gattttcagc gtattaacgc gaatgctgcg gcgcaacggg cgctgacagg cggcaagaaa
     3121 atcatcaaat gagagcgtcg aaggcatagc ttcgcgcatt tgtgtcagaa acgcgtccgg
     3181 gaaataaacg gtgtgttggg ccacgagcat gtaccactgg caaaaaaaca ggcgcgcagt
     3241 ttagcataaa cgctccggcg caggcacgcc ggagcagtgg gcattatttg ggaagcgcag
     3301 ttccccattc acgccactct ttcggttcac tttcctgcaa caggaagtgt ttgccttcct
     3361 gggctttcgg tgccagtggc gtacccggag gcgtggcgaa ggcgatgccg ccacggataa
     3421 actggttaaa ggtgccggtt ttcactacgc cgcccgtcag accaaagtcc agactgtaac
     3481 ccgatgccaa ccagaagacg gaattgttac gcaccaggtg ttgatagcgt ttactgatgc
     3541 gcatcgcaat catcacgcga tctgacaatg tccccagcgt cattccggta accgtaccta
     3601 cttccagacc acggaacagc acaggcgtac caatacctaa cgaaccggct tccggtgctt
     3661 cgacaataat gcttaagcca tccaggtaac gcgaatcagt aatagtagct tcttgcaact
     3721 caaagtcacg gcgaggatta ccccgacctg gttcgacatt gatatacggc tggaggatag
     3781 tatctagatg ctcaacgccc gtggctgaaa tttgcggtgt gaccaccgag aagcgcgtac
     3841 cgccgcgggc gaaggtctgg acatactccg gatagagcac cgcctttgcc tgtacttcat
     3901 tgcgcgcggt aatcagatcc agcgtctgga tttgcccgat atcaataccg agatagcgaa
     3961 ttggcatccc gaccgccagt tttccggcat cgaaagcgtg aagcgtaatc tgcccactaa
     4021 ccgcacgggc cgctgtttcg gaagcataca gaattcgttt gtcgccttta cgctgactgg
     4081 cgctggcacc gctgaggtta tcgaagctaa tggctccctt taatgctctg gagagcgggg
     4141 atgcctgtac ggtcaggcca ctaccattca gctgaacttt cgccccgcct tctgcccaga
     4201 acacgctatt gctggtcaga aggttgcgat actccggctt aatatgcaga tcgatatcaa
     4261 acgcgttagc tcgcggacgc acggtaatca cttcaccaac ttcaaattta cggtacagca
     4321 ctaccgatcc tgcctgcaca tccggcagcg tctctgcact caaactcacg gttgtggtgg
     4381 gtaaatcgct aaggctgttc tccagcgctt tttccagatt ggcatacagt ggatagctgg
     4441 ctttcatctc acctttatcg cctggcagaa tacgtatccc gccgttaatc cattctgagg
     4501 cgctggcacc aagaaactca acgccatcca gccccacctt cacgtcgaca cggctgttga
     4561 cgacaaattt gctatcgcct tttaccagtt cgcgatgctg aggctcgatg gcgacggtaa
     4621 aggtaacgcc tttgctggtg agtttacgat caataacctg gcctacctgc acgccgtgaa
     4681 gaatgagcgg ctgacccgta tcaataccgt aactttccgg cgcggtcagg gtcagcgtca
     4741 gaacatcagg ttcatgcagc agtgcttttt cgcctggcac aacaacgaac tctttgcgtg
     4801 gctcgccatc gccgggtacc aactcgaagg ttttgccagt cagcagggcg ctgagattgg
     4861 catcgctaag ggataatttc ggattacgta attcgatgcg ggtattttca cgaagcaggg
     4921 taacgacgct gggatcaact gtcatttccc cagtgacttt accaccagga tttaaatcca
     4981 gtttagttag ctgtccgact tccagtccct gatacattaa tggcgtcgag tcggcggtta
     5041 atccggcccc actcggcagt tccagtttga ttattacgcc acgctggcta tgggcgaggt
     5101 cttcatacag accaaaggta tcttccgcct cggcaggttt cgactcttct ggtgaatcga
     5161 aggcaatcgc accgttaacc agtgccgcca ggctttccag tttcaccttc gcgccactga
     5221 tactgacatt ggcatcaacg ccggaaacgt tccagaaacg gctacctttt ttcaccagat
     5281 cggtaaaacg ccgctcgatc aggacatcaa tcaccacgcc ttgtttgttg ggattgatgg
     5341 catagtcgta gacttttccc accgggatct tgcggaaata gaccaatgaa ccgctgttca
     5401 gcgaaccgag atcgggggct tgcaggtgga tcatcagatc gccattgttc agccgatatt
     5461 tcggttgggt atcgagtgcg acaaagtgat cctgctcttt acctgtaccc ggcatcatgc
     5521 cgatatagtt cccaccgacg agggcgtcca gcccggagac acctgccaac gatgcttttg
     5581 gcgtcaccag ccagaattga gtctcttcgc gcagcgcatc tttcatatcg gacttgatgc
     5641 tgaccttgac ttcaatctta cgaagatcgt cgctgaggct gatatcctgc actgttccga
     5701 cttcaacgcc ctgataacga acaggcgtac ggcccgggac aataccatcc gccgacataa
     5761 agtcgatggt gacggtatta cttcggtcct gataactgtc ccaaatcagc caaccggcaa
     5821 tcattagcgc gatgaaaggc agcagccaga aaggtgagat acggcgttta tttttaatct
     5881 gcgcttcagt cgtcgaagcg ggcgtttcct gactcatgtg catcccaaag taagcggctg
     5941 tccagccatt ccacagcaag aatagtcaaa attacggctg cgccaaaata aaacgcagcc
     6001 ggtcccatag taaaagcgag gatctgatcg cgattaatca gcgacatggt taaagatatg
     6061 acaaacaggt cgagcatcga ccagcgtcca atccaggtca ccatccgcag taacagaatg
     6121 cgtgtgcgta aaccttgctg gcatttaaaa tgaatgctca acagtaaagt gaacatgacg
     6181 atcactttag taaacggtac cagaatactg gcgataaaca cgattccggc gaccgcaatg
     6241 ttgctacttg ccagcgacat aatcccggaa agaatcgtat cttcctgccg gccaccattc
     6301 agataaatga tagaaatagg caataggttg gctggtaaca acaaaacgat tgacgctaac
     6361 agcgccgccc agcatttttg caaactatga cgacgacgca ggcgtagcgg gatatggcaa
     6421 cgcgggcagc gaccacgctg atcaggatag ccggtaaaat ggcacccaag acagacacga
     6481 agtttctcgt ccctacgcgt agcggggcgc tgcggataat atcgctccca cagctcctca
     6541 acattaagat gtgacaacgt caccgtcgtt aaaatcacca acgccacaaa agagaacaag
     6601 ccgacacccg cctggatatg ggcataatcc tgtaccttta tagaagcaac gccaatgccg
     6661 accaggtaga tatccagcat tacccactct ttaagacgct caagcatcag cagtaccgga
     6721 cgtaaattca ttcccagtcg gttaccaaac cataaatatg ctatggagga caccagaatg
     6781 aggggggcac caataacgca gaaaaagacc atcgcccccg ttatcgtatc gccctgtttg
     6841 gtcatttgcc agatgccttg catgacgttt gcgtcgatac gaatacctaa cagccagata
     6901 tgcaacagcg gttcgcccca ggcaaacggc atcagcaaca gcatggtgaa agccattgcc
     6961 gccaggcgcg ttagcgacca gtcgcgcccg tcgcgaattt ttgcctgaca gcgcggacaa
     7021 taggcactct gatgagaatt tatctcgggc aggctaaaca gcatgtcaca ttgcgggcaa
     7081 cgttggtaat caccacgcgg cagttcctcg ccgattgacc tcactgttat cttttttgtc
     7141 ggcgtaattt gtggtgtgtt aagagccatg acgtgccaaa tgtaataaga attaacatta
     7201 atcttaaccc atgatgggaa tgatcttgag cattaacgca tttaatgctt attttaatag
     7261 gctggcagtt ccaggtaaga caaacaagtg attatataat gaacaaaaca gaattttacg
     7321 cggatttaaa tcgcgacttt aacgcgctga tggcgggaga aaccagtttt ctggcaacgc
     7381 ttgcgaacac cagtgcgttg ttatacgagc gtctcactga cgtaaactgg gcaggttttt
     7441 atttgcttga ggacgataca ctggtactcg gaccatttca gggcaaaatt gcctgtgtcc
     7501 ggatacccgt cgggcgcggg gtgtgcggca ctgcggttgc ccgcaatcaa gtgcagcgta
     7561 tcgaggatgt tcacgcgttt gacgggcata ttgcctgtga tgcggcgagt aattctgaaa
     7621 ttgttctgcc gctggtggtg aaaaatcaga ttattggtgt tcttgacatc gatagtaccg
     7681 tcttcggtcg ctttacagac gaggacgagc aaggcttacg tcagcttgtg gcacagcttg
     7741 aaaaagtgct tgcaacgacg gattacaaaa aattctttgc gagcgtcgca ggataatcaa
     7801 cggataacgt agcatttact ggtagcgtca ttataatgac gcctgttcat gcctgcgctt
     7861 gttggctacg tccgttgtaa tcaggaaatt tcatggaaaa tcaacctaag ttgaatagca
     7921 gtaaagaagt aatcgcgttt ctggccgaac gttttcccca ctgtttcagt gcggaaggtg
     7981 aagcgcgtcc gctgaaaatc ggtatttttc aggatttggt cgatcgtgtt gccggggaaa
     8041 tgaacctgag caaaacgcaa ttacgatccg ctttacgtct ctacacttcg agctggcgtt
     8101 atctttacgg tgttaaaccc ggcgcaacgc gtgtcgatct tgacggcaac ccatgcggtg
     8161 agctggacga gcaacatgta gagcatgctc gcaagcagct tgaagaagcg aaagcgcgtg
     8221 ttcaggcaca gcgtgctgaa cagcaggcga aaaaacgcga agctgccgca gctgctggtg
     8281 agaaagaaga cgcaccgcgc cgcgaacgca agccacgtcc gaccacgcca cgccgcaaag
     8341 aaggcgctga acgtaaacct cgtgcgcaaa agccggtaga gaaagcgcca aaaacagtaa
     8401 aagcacctcg cgaagaacag cacaccccgg tttctgacat ttcagctctg actgtcggac
     8461 aagctctgaa ggtgaaagcg ggtcaaaacg cgatggatgc caccgtatta gaaatcacca
     8521 aagacggcgt ccgcgtccag ctgaattcgg gtatgtcttt gattgtgcgc gcagaacacc
     8581 tggtgttctg aaacggaggc cgggccaggc atgaacatgt tttttaggct taccgcgtta
     8641 gctggcctgc ttgcaatagc aggccagacc ttcgctgtag aagatatcac gcgtgctgat
     8701 caaattccgg tattaaagga agagacgcag catgcgacgg taagtgagcg cgtaacgtcg
     8761 cgcttcaccc gttctcatta tcgccagttc gacctcgatc aggcattttc ggccaaaatc
     8821 tttgaccgct acctgaatct gctcgattac agccacaacg tgctgctggc aagcgatgtt
     8881 gaacagttcg cgaaaaagaa aaccgagtta ggcgatgaac tgcgttcagg caaactcgac
     8941 gttttctacg atctctacaa tctggcgcaa aagcgccgtt ttgagcgtta ccagtacgct
     9001 ttgtcggtac tggaaaagcc gatggatttc accggcaacg acacttataa ccttgaccgc
     9061 agcaaagcgc cctggccgaa aaacgaggct gagttgaacg cgctgtggga cagtaaagtc
     9121 aaattcgacg agttaagcct gaagctggca ggaaaaacgg ataaagaaat tcgtgaaacc
     9181 ctgacacgcc gctacaaatt tgccattcgt cgtctggcgc aaaccaacag cgaagatgtt
     9241 ttctcgctgg caatgacggc gtttgcgcgt gaaatcgacc cgcataccaa ctatctttcc
     9301 ccgcgtaata ccgaacagtt caacactgaa atgagtttgt cgctggaagg tattggcgca
     9361 gtgctgcaaa tggatgatga ctacaccgtt atcaattcga tggtggcagg tggtccggca
     9421 gcgaagagta aagctatcag cgttggtgac aaaattgtcg gtgttggtca aacaggcaag
     9481 ccgatggttg acgtgattgg ctggcgtctt gatgatgtgg ttgccttaat taaagggccg
     9541 aagggcagta aagttcgtct ggaaatttta cctgctggta aagggaccaa gacccgcact
     9601 gtaacattga cccgtgaacg tattcgtctc gaagaccgcg cggttaaaat gtcggtgaag
     9661 accgtcggta aagagaaagt cggcgtgctg gatattccgg gcttctatgt gggtttgaca
     9721 gacgatgtca aagttcaact gcagaaactg gaaaaacaga atgtcagcag tgtgatcatc
     9781 gacctgcgta gcaatggcgg tggggcgttg actgaagcgg tatcgctctc aggtctgttt
     9841 attcctgcgg gtcccattgt tcaggtccgc gataacaacg gcaaggttcg tgaagacagc
     9901 gataccgacg ggcaggtgtt ctataaaggc ccgctggtgg tactggttga ccgtttcagt
     9961 gcttcggctt cagaaatctt tgccgcggca atgcaggatt acggtcgtgc gctggttgtg
    10021 ggtgaaccga cgtttggtaa aggcaccgtt cagcaatacc gttcattgaa ccgtatttac
    10081 gatcagatgt tacgtcctga atggccagcg ctgggttctg tgcagtacac gatccagaaa
    10141 ttctatcgcg ttaacggcgg cagtacgcaa cgtaaaggcg taacgccaga catcatcatg
    10201 ccgacgggta atgaagaaac ggaaacgggt gagaaattcg aagataacgc gctgccgtgg
    10261 gatagcattg atgccgcgac ttatgtgaaa tcaggcgatt taacggcctt tgggccggag
    10321 ctgctgaagg aacataatgc gcgtatcgcg aaagatcctg agttccagaa catcatgaag
    10381 gatatcgcgc gcttcaacgc tatgaaggac aagcgcaata tcgtttctct gaactacgct
    10441 gtgcgtgaga aagagaataa tgaagatgat gcgacgcgtc tggcgcgttt gaacgaacgc
    10501 tttaaacgcg aaggtaaacc ggagttgaag aagctggatg atctaccgaa agattaccaa
    10561 gagccggatc cttatctgga tgagacggtg aatatcgcac tcgatctggc gaagcttgaa
    10621 aaagccagac ccgcggaaca acccgctccc gtcaagtaat atcaatcagg cacaagaaat
    10681 tgtgcctgat tttttaacag cggcaagatg ccgtaaatca gatgctacaa aatgtaaagt
    10741 tgtgtctttc tggtgactta cgcactatcc agacttgaaa atagtcgcgt aacccatacg
    10801 atgtgggtat cgcatattgc gttttgttaa actgaggtaa aaagaaaatt atgatgcgaa
    10861 tcgcgctctt cctgctaacg aacctggccg taatggtcgt tttcgggctg gtactgagcc
    10921 tgacagggat acagtcgagc agcgttcagg ggctgatgat catggccttg ctgttcggtt
    10981 ttggtggttc cttcgtttcg cttctgatgt ccaaatggat ggcattacga tctgttggcg
    11041 gggaagtgat cgagcaaccg cgtaacgaaa gggaacgttg gctggtcaat actgtagcaa
    11101 cccaggctcg tcaggcaggg atcgctatgc cgcaagtggc tatctaccat gcgccggaca
    11161 tcaacgcttt tgcaaccggt gcgcgccgtg atgcctctct ggttgctgtc agcaccggtt
    11221 tgctgcagaa catgagcccg gatgaagccg aggcggtaat tgctcacgaa atcagccaca
    11281 tcgccaatgg tgatatggtc accatgacgc tgattcaggg cgtggtgaac accttcgtta
    11341 tctttatttc ccgtattctg gcgcagcttg ccgcgggttt tatgggcgga aatcgtgatg
    11401 aaggtgaaga gagcaacggc aacccgctga tctactttgc ggttgcaacg gttctggaac
    11461 tggtgtttgg tattctggcg agcattatca ccatgtggtt ctcgcgtcat cgtgaattcc
    11521 atgctgatgc cggttcggca aaactggttg gtcgcgagaa aatgattgcc gcgctgcagc
    11581 gcctgaaaac cagctatgaa ccgcaagaag caaccagcat gatggctttc tgcattaatg
    11641 gtaagtcgaa atcgctcagt gagttgttca tgactcaccc gccgctggat aaacgaattg
    11701 aagctctg
//
LOCUS       DAJVTY010000130        11691 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVTY010000130 DAJVTY010000000
VERSION     DAJVTY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.130"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..182
                     /locus_tag="OQQ66_003109"
     CDS             <1..182
                     /locus_tag="OQQ66_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1905949.1"
                     /translation="FISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGTNTLL
                     ACCCLLRPEEVNSEY"
     gene            172..345
                     /locus_tag="OQQ66_003110"
     CDS             172..345
                     /locus_tag="OQQ66_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1905950.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(394..1466)
                     /locus_tag="OQQ66_003111"
                     /pseudo
     CDS             complement(394..1466)
                     /locus_tag="OQQ66_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1538..4282)
                     /gene="torS"
                     /locus_tag="OQQ66_003112"
     CDS             complement(1538..4282)
                     /gene="torS"
                     /locus_tag="OQQ66_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS1905951.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4365..5354
                     /gene="torT"
                     /locus_tag="OQQ66_003113"
     CDS             4365..5354
                     /gene="torT"
                     /locus_tag="OQQ66_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1905952.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5327..6019)
                     /gene="torR"
                     /locus_tag="OQQ66_003114"
     CDS             complement(5327..6019)
                     /gene="torR"
                     /locus_tag="OQQ66_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1905953.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6139..7311
                     /gene="torC"
                     /locus_tag="OQQ66_003115"
     CDS             6139..7311
                     /gene="torC"
                     /locus_tag="OQQ66_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1905954.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7311..9891
                     /gene="torA"
                     /locus_tag="OQQ66_003116"
                     /pseudo
     CDS             7311..9891
                     /gene="torA"
                     /locus_tag="OQQ66_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9888..10487
                     /gene="torD"
                     /locus_tag="OQQ66_003117"
     CDS             9888..10487
                     /gene="torD"
                     /locus_tag="OQQ66_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1905955.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10580..10885)
                     /gene="cbpM"
                     /locus_tag="OQQ66_003118"
     CDS             complement(10580..10885)
                     /gene="cbpM"
                     /locus_tag="OQQ66_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1905956.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10885..>11691)
                     /gene="cbpA"
                     /locus_tag="OQQ66_003119"
     CDS             complement(10885..>11691)
                     /gene="cbpA"
                     /locus_tag="OQQ66_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1905957.1"
                     /translation="EPDAEARFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFH
                     HGDGQSFNAEDFDDIFSSIFGQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTIS
                     YNLPVYNAFGMIEQEIPKTLNVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHI
                     APHPLFDIVGQDLEIVVPVSPWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGK
                     GLVSKKQTGDLYAVLKIVMPPKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2789 a         3029 c         3109 g         2764 t
ORIGIN      
        1 tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga
       61 ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa
      121 ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac agtgaatatt
      181 gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca aaaaatcgcc
      241 gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa
      301 atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac
      361 cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg gcgcttgtgg
      421 aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg catacattgc
      481 gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc agccagacaa
      541 cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg ttgaggctac
      601 cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta cctgaattaa
      661 tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc cgatacaact
      721 ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca tcagcgcgcc
      781 atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa tcagcaaaac
      841 ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca tttttacgtc
      901 catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg gcatcaataa
      961 cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag gaatatccag
     1021 ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc cgattaattc
     1081 agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta atagactgat
     1141 gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa ctgacctgtt
     1201 gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc
     1261 ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca ataccagaac
     1321 gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc
     1381 gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc
     1441 cagcgggttc tttttttctc tgccatgatt ttgtcagtcc gttaagttgc ataccaaatg
     1501 ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct
     1561 tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg
     1621 cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaaac
     1681 tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta tcctgactgg
     1741 cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc
     1801 attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca
     1861 gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt
     1921 cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc tggcgcagcg
     1981 tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca
     2041 gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc agtgcggcag
     2101 caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca acagcaacaa
     2161 cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc gggttatctt
     2221 caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt tttggcacgg
     2281 gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc tccggcgtgc
     2341 tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata gtcagtccca
     2401 gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt tctgccagtt
     2461 tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc tcgccatcag
     2521 tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg ctcaacaggt
     2581 tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt acatcgtcgg
     2641 caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg
     2701 tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca ttcttgccac
     2761 ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccag
     2821 agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta tctgccagca
     2881 gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca
     2941 gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt cggtgttcta
     3001 tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc tgttcacggt
     3061 ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg atggtatcca
     3121 gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc agcagccgtt
     3181 gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc acgcgccaaa
     3241 ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg tattgcccgc
     3301 gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca atggtgtcga
     3361 ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt tgtgcgagag
     3421 tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa tcgctatatt
     3481 ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct tcaatgcgta
     3541 tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc attggtgcat
     3601 ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc agagcgctaa
     3661 ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg
     3721 cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg gcctgggtcg
     3781 ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg atctcatcgg
     3841 cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc agacgccgcc
     3901 ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag
     3961 cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt
     4021 gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt
     4081 tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg
     4141 taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc
     4201 atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc
     4261 ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt gagtcgcaaa
     4321 gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc gtactgctat
     4381 ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg ttgcgctggc
     4441 atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa cgcgcatgga
     4501 aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg aactatggta
     4561 tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc gaccagtgta
     4621 aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt cccgacctgc
     4681 aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat gatcccctgg
     4741 tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg cgatatttgg
     4801 tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc gataatgccg
     4861 ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc ccggtgcgta
     4921 ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg ttgcaggaga
     4981 tgctggaacg ccacccagaa atcgacgtcg ttgccggaac ggccattgcg gcagaggcgg
     5041 caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt tatctttcac
     5101 atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc gatcaaatga
     5161 tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg caatcggtgt
     5221 ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac cgtgaacata
     5281 ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag cacacatcag
     5341 cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg agtttatgac
     5401 gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc cgacgcgcag
     5461 aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc acaaatgcca
     5521 ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc tccagcgtat
     5581 ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga gtgtgcggtt
     5641 gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact accagttcgc
     5701 gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca acaatacggt
     5761 caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt tctcgcaggg
     5821 cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga attaaatcta
     5881 ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg gaaacggtat
     5941 acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca tcttcaacaa
     6001 taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct gccgtaagcc
     6061 gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc actaaaccat
     6121 aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc cagtgctcgt
     6181 tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct gattgtattg
     6241 ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg ccacagtatg
     6301 caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc cggcgtgcga
     6361 gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa gcgcaaactg
     6421 gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac acctgaaaaa
     6481 ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa agaaaacaac
     6541 tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa gcagcatcct
     6601 gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat cgactgtcat
     6661 aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca gttcgatgag
     6721 ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat taagccaatt
     6781 tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc ggaagtgaaa
     6841 gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac ggaaagcgcc
     6901 ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc ctcgattcgt
     6961 ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga caccaatacc
     7021 gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt gaacgatatc
     7081 aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca gtgccacggc
     7141 gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa cggcatgatt
     7201 ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct gcaaatgaat
     7261 gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata atgaacaata
     7321 acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc ttaacagtcg
     7381 ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg caagcggcgg
     7441 atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc cgcgcgacgg
     7501 tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat ccgtcgaaaa
     7561 tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg attgccggat
     7621 cacgtacaca acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag
     7681 cgccatctca gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat
     7741 gaagccctcg acatgttcta tgaagaactg gaacgcgtgc agaaaactca cgggccgagt
     7801 gccttgctga ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg
     7861 ctggcgaaag ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc
     7921 ggtgctgcgc aggtgatcct gccgcgcgta gtcggttcga tggaagtgta tgaacagcaa
     7981 acctcctggc cgctggtatt gcagaacagc aaaaccatta tgctgtgggg ctccgatttg
     8041 ctgaaaaacc agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgag
     8101 caactaaaag cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc
     8161 acatccaccc atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa
     8221 actgacgtgc cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac
     8281 aaaaacttcc tcgctaacta ctgtgtaggt tttgagcagt tcctgccgta tctgctgggt
     8341 gagaaagacg gtcagccgaa agatgccgca tgggctgaaa aactgaccgg cattgatgcc
     8401 gaaaccattc gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc
     8461 tggtgcgtgc agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg
     8521 gcgatgctgg ggcaaattgg cctgccaggt ggtggttttg gttttggctg gcactacaac
     8581 ggcgcaggca cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg
     8641 attccgcctg ttcacgacaa cagtgactac aaaggctaca gcagcaccat tccgattgcc
     8701 cgttttatcg atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta
     8761 aaactgccgc cactgaaaat gtgtattttt gccggaacta acccattcca tcgccatcag
     8821 cagatcaacc gcattattta aggctggcgc aagctggaaa cggttatcgc catagataac
     8881 cagtggacct caacctgccg ctttgccgat atcgtactgc ctgcgaccac gcagtttgag
     8941 cgtaacgatc tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag
     9001 gtggtgccgc cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt
     9061 cgctttaatc gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc
     9121 atctggcagg aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat
     9181 gacttctgga ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac
     9241 caggcattcc gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag
     9301 atctactcga aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg
     9361 tttgagaaaa tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg
     9421 caatctgtgc atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt
     9481 cagcaatata cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg
     9541 cgcggtattc gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca
     9601 ggggcagtag tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg
     9661 tacgatccag ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg
     9721 ctgaccatcg acatcggtac ttcgcagctg gcgcaggcga ccagtgcgca cactacgctg
     9781 gtggaaattg agaagtacaa tggagcagtg gagcaggtga cggcgtttaa cggccctgtg
     9841 gagatagtgg cgcagtgcga atatgttccc gcgtcgcagg tgaaatcatg accacgctga
     9901 cagcacaaca gattgcctgt gtttacgcct ggctggcgca gttgttctcc cgtgaactgg
     9961 acgatgaaca actgacgcaa atcgccagtg cgcagatggc ggaatggttt tcgttgctga
    10021 aaagcgaacc gccgctggct gcggcggtga atgagctgga aaactgtatt gccacgctga
    10081 cagtacgtga cgatgcccgt ctggaactgg ccgctgactt ttgcggcctg tttttgatga
    10141 ctgataagca ggcggcgctg ccgtatgcat cggcctacaa acaggacgag caagagatta
    10201 aacgcttgtt agttgaggca gggatggaaa ccagcggcaa tttcaacgaa ccggcagacc
    10261 atctggcgat ctatctcgag ttgttgagtc atctgcattt ttcgctggga gaggggaccg
    10321 ttcctgcgcg aagaatcgac agtttgcggc aaaaaacact gacggcgctg tggcaatggt
    10381 taccagagtt tgctgcgcgt tgccatcagt atgacagctt tggtttttac gcggcactaa
    10441 gccagttatt gctggtgtta gtggagtgcg accaccaaaa caaataacgt cgtttatgcg
    10501 cctgaaaaga cgcgtttagc gtcgcatcag gcattatggc gcagttgccg gatgcgtcat
    10561 cacatccggc aaacacacat cacggatgag ctacaaaccg ggaaagccgc tggcgtagca
    10621 ggcggttttc ctgcttcagg tgtgcaatat catccattaa cgtcagcgcc accgcgatcc
    10681 ccggccagtc cagagccagt tcatgacgca ggcgtaccgc gcgttgcacc acaatggcgg
    10741 catggtcgtc aaatacccag gttgtttcct ggatctcacg cggttcaacc acccccaaac
    10801 cgacaatttc attcaactcc tcttcagaga tgccggtatg caggcaaaat tcggtaatag
    10861 taaaagtcac cgtaacatta gccatcatgc tttcccccaa tctttacgtg gatcaaaaga
    10921 cgactgggcg tctgccagtt gctgccacag cgcggcagtg ttttcatccg gtttcggcgg
    10981 catcacgatt ttcagtaccg catacagatc gccggtctgt tttttgctca ccagaccttt
    11041 gcctttaacg cgcaatcgtt gcccggcctg gctgcctggc gggatagtca gcaaaatgct
    11101 ttctttcagt gttggaacgg tgactttagc acccagcgcc gcttcccacg ggctaaccgg
    11161 caccacaatt tccagatcct ggccgacaat atcgaacagc ggatgtggcg caatatgaat
    11221 caccagccac aaatcgccat ttggaccacc gttttcgccc ggcgtcccct ggcctttcag
    11281 acggatgcgt tgaccattgc cgacgcccgc cgggatcttc acattcagcg ttttcgggat
    11341 ttcctgttcg atcatgccaa aggcgttata aaccggcagg ttatagctga tggtacgctt
    11401 atgctcagtc agcgtttctt cgaggaatac cgccacttcg atttcaatat cgtggccgcg
    11461 tgcggcgggg cgttgacggc tctggcgggc atgctgaccg aaaattgacg agaagatatc
    11521 gtcaaaatct tcggcattaa aactctgacc gtcgccatga tggaactgac ggttaaattg
    11581 cggatcgttg cgatgttgcc acatctgatc atactcagcg cgacgttgtt catcacttaa
    11641 cacttcccag gcttcagcga cctctttgaa gcgggcttcg gcatccggtt c
//
LOCUS       DAJVTY010000131        11565 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVTY010000131 DAJVTY010000000
VERSION     DAJVTY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.131"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..974)
                     /locus_tag="OQQ66_003120"
     CDS             complement(<1..974)
                     /locus_tag="OQQ66_003120"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1905958.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRENIMLEGFTLFT"
     gene            complement(1183..1842)
                     /locus_tag="OQQ66_003121"
     CDS             complement(1183..1842)
                     /locus_tag="OQQ66_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1905959.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2169..2525)
                     /locus_tag="OQQ66_003122"
     CDS             complement(2169..2525)
                     /locus_tag="OQQ66_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1905960.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2592..2886)
                     /gene="yebG"
                     /locus_tag="OQQ66_003123"
                     /pseudo
     CDS             complement(2592..2886)
                     /gene="yebG"
                     /locus_tag="OQQ66_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3020..4198
                     /gene="purT"
                     /locus_tag="OQQ66_003124"
     CDS             3020..4198
                     /gene="purT"
                     /locus_tag="OQQ66_003124"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1905961.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEERLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4254..4895)
                     /gene="kdgA"
                     /locus_tag="OQQ66_003125"
                     /gene_synonym="eda"
     CDS             complement(4254..4895)
                     /gene="kdgA"
                     /locus_tag="OQQ66_003125"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1905962.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4932..6743)
                     /gene="edd"
                     /locus_tag="OQQ66_003126"
     CDS             complement(4932..6743)
                     /gene="edd"
                     /locus_tag="OQQ66_003126"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1905963.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6978..8453)
                     /gene="zwf"
                     /locus_tag="OQQ66_003127"
     CDS             complement(6978..8453)
                     /gene="zwf"
                     /locus_tag="OQQ66_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1905964.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8791..9660
                     /gene="hexR"
                     /locus_tag="OQQ66_003128"
                     /gene_synonym="yebK"
     CDS             8791..9660
                     /gene="hexR"
                     /locus_tag="OQQ66_003128"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1905965.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9788..11230
                     /gene="pyk"
                     /locus_tag="OQQ66_003129"
     CDS             9788..11230
                     /gene="pyk"
                     /locus_tag="OQQ66_003129"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1905966.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11290..11508)
                     /locus_tag="OQQ66_003130"
     CDS             complement(11290..11508)
                     /locus_tag="OQQ66_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1905967.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2803 a         2976 c         2888 g         2898 t
ORIGIN      
        1 gtaaacagcg taaacccttc cagcatgatg ttttcgcgtg gcggaattaa ctcttcccac
       61 tgttgctcat cacgcaggcg ggtacggtat aagccaaagt ttttgccgtg gcggttggaa
      121 cgcagataaa accgatgctg gtagtgatca aggctgtatt cgtgatcttt gcggcgcggc
      181 agaaaaacaa acggctcggc atcggccatt tccgcgtcca gcaggcgaac ttcactggtg
      241 gtggcgctgg ccaaatgaat gactacatag tgcttcgagg tcgttttatg caggctgacg
      301 taataggtat cgtctttttc ttcgtagatc agtttatctt gcgatgctgg cgtaccgatg
      361 gcgtgacgcc agacctgata aggcagcagc gtcaccggat gcttgcgaac atagtagaaa
      421 gtccaggagt catttgccca gacaaagctg ggttcaacgt tatccagcag ttccgggtac
      481 cagttaccag tttccagatt acgaaaacga atgccgtact ggcgtcggga aagaaaatct
      541 tctgccagcg ccataatggt gttatcgggc gtaatcgcca ttccgcccat cgaataaaac
      601 tcactatgag ctgcgcgctt attggcatcg agcaatgttt cccactcatc ccactcttca
      661 ctgaatgccg attgacgctg gtagatagca tattcacagc ctggttcata aatatgccga
      721 tagcggtagc catttttgat gtagggcgca gaaacttctc gttgcggaat gcggtcgatg
      781 atttctttta agatgcgatc ctgcaaggct tgttgtgagg ccatcacccg atgaccgtaa
      841 ctattttctt gttgcaggta gtccaggact tctggctgag aacgcgtatc gtcccgcagc
      901 cagtagtaat tatcgatgcg cgtatcgcca tgaagcgtca tggcgtgggg aatgcgggcg
      961 gcttttggta gcatgttatt gttctttctg gttgaaacat cttataaggg tggcaaaact
     1021 caccggggat gcaagcgaaa caggggagtc attgcttaga tgatgacagg taatggcgcg
     1081 gatatcgaat gttatgcaga cagagaaaat cagcctgttc aaatggctgt gcgattctgg
     1141 atagcccgaa atagtcaact tcaggctatc cagagagcgg aattattccg ccaaagtgcg
     1201 tttttgctgt tcgagatcgc gttcaatgcc gtcacgaaca tcctggggga ttttcagcgc
     1261 gtcacccagt gcattcaggt aactgcgttc cataaaatgg tcaatatcaa tagccgcgca
     1321 actcaggaaa tagatttcca gcgcctcttc ttcattgcgg accccggtag ccaagcgttg
     1381 tggatccagc ggttgttcga ttgcctgctc aatgagtaca cgcccctgct cttccacgcc
     1441 ggcttcacgc aattgctggt cgattgccgc acgttctttg gcatcaatat gaccatcact
     1501 tttagcggca aagaccagcg caaggatcaa acgttctgta cgctcatcca gcggcgtact
     1561 ttgcgcgcca aactgcggtt cgtcctgatg cgccgcgcga attttatctt tgtatttatt
     1621 ccacagcacc gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg tgccatattt
     1681 cgtaagaagt ttacgtgctg atttatttgc gaccagcagc ccagccagtc cgcctaatgc
     1741 gcctggcact aacagtttgc ccaatccctg atccgcagac gaggagatag aagaactgct
     1801 ttgcccaagc agggattgca gttgatttaa ccagttagcc atatttgctc ctcaataacc
     1861 atttttagtg cctgtagcat agcggatgag gatgtcagaa aatgtatatc gagacaaaag
     1921 atgcgcaaat aagcggggcg gtaggccgtt attcaaagaa attcgcgcca ggtaaggtat
     1981 ttactgtata tcaggtgata agggtatttt aggtgaaagt agtttgtcgt tttgagtggc
     2041 attgtttgat gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc tacgattgtg
     2101 gtgcgttctg gggcggacaa ggcactcgcg ccgcatccgc caacggtcaa tcgaattatc
     2161 agaagccatt aacgccgctg atattccgcc attcccgctt tgcagtccac gctgacctgg
     2221 taatgaatat cggcactttt accacgcacg gttagcggta ctgaccattt atcatcttta
     2281 ccctgaatgt cctgcaaact gacccaagcc acgggatcgg cctgaccgac aattttttga
     2341 tcatctgccc aacgcgccac gcgattttgt tgataatcac gttttacgct cgcggcaatt
     2401 ccggcagcat ccagaccttc acactttggg aaagtgaccg acttgctggt ttcattattg
     2461 gcagcgaaaa ctgatgcgca ggcagaaacc aacaacagcc ctaaaaacgc ccctcttttt
     2521 ttcatgtttt tctccatagc acaatgattc aggagaaagc atggtacaaa ttgtcaggag
     2581 cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt cggattcagc accgacgacc
     2641 tgtgggtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat ctttttgttc
     2701 tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg gagaattagt
     2761 cagccaggtg tcaagcagat ccgccgtatc aagcattttg tcataagcat cggcttcctt
     2821 tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt taacttcaac
     2881 cgccatttgc agcctctcat aataactgtg attttataca gtatatttct tttcggttga
     2941 gaaatcaaaa tcaacaataa agacacacgc aaacgttttc gtttatactg cgcgcggaat
     3001 taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg
     3061 cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct
     3121 cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca
     3181 tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga
     3241 aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc tgatccaact
     3301 tgaagaggaa agactgaatg ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg
     3361 cgagggtatc cgtcgcctag cggcagaaga gctgcagctg cccacttcca cttatcgttt
     3421 tgccgatagc gaaagccttt tccgcgaggc ggttgctgac attggctatc cctgcattgt
     3481 aaaaccggtg atgagctctt ccggcaaggg gcagacgttt attcgttctg cagagcaact
     3541 tgctcaggca tggaagtacg ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt
     3601 tgaaggcgtc gttaagtttg acttcgaaat taccttgcta accgtcagcg cggtggatgg
     3661 cgtccatttc tgtgcaccgg taggtcatcg ccaggaagat ggcgactacc gtgaatcctg
     3721 gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg cccgtaaagt
     3781 ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct gtagtgatga
     3841 ggtgattttc agtgaggtct cccctcgtcc acatgatacc gggatggtga cgttaatttc
     3901 tcaagatctt tcagagtttg ccctgcatgt acgtgccttc ctcggacttc cggttggcgg
     3961 gatccgtcag tatggtcctg cagcttctgc cgttattctg ccacaactga ccagtcagaa
     4021 tgtcacgttt gataatgtgc agaatgccgt aggcgcagat ttgcagattc gtttatttgg
     4081 taagccggaa attgatggca gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt
     4141 tgttgacgcc attgaacgcg cgaagcacgc cgccggacag gtaaaagtac agggttaaac
     4201 ccggcaaaaa aacgctacaa aaatgcccga tcgtagatcg ggcgttttga cttttacagc
     4261 ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc
     4321 agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt cagcgccagg
     4381 tagtcacggt agttagccgg agaaatacca cccgtcgggc agaaacggac ctgggagaac
     4441 ggacccgcga tcgcctgcag ggctttcacg ccgccgttag cttcagccgg gaagaatttg
     4501 aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct gatccccgga
     4561 attagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag acccgggcta
     4621 attgcgaact gtgcacccgc ttcagtgact tctgccagct gctgtggatt cagcaccgta
     4681 ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc gtcaactgca
     4741 cactcggtac gcagagtcac ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc
     4801 atcggcactg cgtgttccag tttttttacc acgataaccg gtacaaccgg gccggtggtc
     4861 aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg cctgattaca
     4921 aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga cagtttttca
     4981 cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg
     5041 tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt
     5101 ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct
     5161 tctggtgtta cgtggatagc tgacggcact ttacctgaag cgccggagag tcgtccatcg
     5221 gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg catgagttta
     5281 tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac gacacaatcg
     5341 cggtccagca aacccgcttc aaaggccggc ataacgtcat gctggctttc aaaaacaacc
     5401 gctggcgctt caatcacctg gttctcaacc ggcacggcag aggttttcat aaccgcacgg
     5461 cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa
     5521 gcgatcacat tgctgtcgag tgatttttcc gccccttccc gccagtccag ttcaccatta
     5581 ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt attgacatct
     5641 tcatgcagca ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc acctgccgcc
     5701 tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag cggtacaaca
     5761 tcagaaaggt cagagaagtc atcccagtta atctgaatac cggccgcgcg cgccatcgcc
     5821 accaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac gataccgttc
     5881 accaccactt tctcatcgat catcttaccg atcggcatcc attcattacc attaccggtc
     5941 atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga
     6001 tgaacaaaag aagagcctgg cgactgcatc cccataaatt ccaccaccat ctggttggtg
     6061 ttggcagtac cgtagaaagt acatgttccc ggcgcatggt aagacgcggc ttctgactcc
     6121 agtaaggcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg cactttttct
     6181 ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg caaatgacca
     6241 aacgacaggg ctgccatcgt cagacccggg acaatcttgt cgcacacacc gaggaacaga
     6301 gcaccatcaa acatgttatg ggacagcccc accgccgcag acatcgctat cacttcgcgg
     6361 cttagcagcg acaattccat tccatcctgc ccctgggtga caccatcaca catcgccgga
     6421 acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg aatgatttct
     6481 ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt tataggaggt gatgatggcg
     6541 atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca ggcagcgaaa
     6601 ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt
     6661 tctatccggg cgagataagc agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt
     6721 acgcgtaaca attgtggatt cataaaggct cctgaaattg agttgtcaga gcaggatgat
     6781 tcacaacgcg tttcattcag aggatttatg agtgaaacgc ctgtaaccgg agctcatagg
     6841 gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct
     6901 gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa aataaccata
     6961 aaggataagc gcagatatta ctcaaactca ttccaggaac gaccatcacg ggtaatcatc
     7021 gccaccgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc atcattgtcc
     7081 atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac ttcatcacga
     7141 cgtacaaaca gtgcctgaat accacgcatg gtttccagca gcagacgttc ataggcatcc
     7201 gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt gatttgcagg
     7261 ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac gccttcatca
     7321 ggttgcagac ggatagtcag tttattctgc ggcagatcct gccacgactc tttaaacaga
     7381 ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg cagacgttta
     7441 ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc gacgcggatc
     7501 gccacgaagg tttctgtatt gctgctcttg ttcgcaccct cttcttccag atatcccggc
     7561 acttttttgc cctgggcgaa gcccgcagta tattgcccac gtacggtttt ttcgcgtacg
     7621 ttggagcggt cgatgcggcg cagggacttc agtactttca ctttttcatc gcggatgctg
     7681 tctgcgctca ggtcagatgg cggagacatc gcaatcatgc agagaatttg tagcaggtgg
     7741 ttctgaatca tgtcacgcat ctgaccggct ttatcaaaat agccccagcg cccttcgatc
     7801 cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc ccagttattc
     7861 acaaacaggg agttagcaaa acgcagcgcc aacaggttca gcaccgtttc tttaccaaga
     7921 tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg atcattgatt
     7981 tcctgcgagg tcgccagcga cgtccccagc ggtttctcca tgactacgcg tgccggttta
     8041 gcattcagtt ttgcctcgcc aagccctttg caaattgcgc caaaagtgct gggcggcatg
     8101 gcaaagtagt taatggtgat acgatttttt tgatccagca tcgcgccgag acggttgaat
     8161 gcagcagtgt cattgacatc gagattacaa aaatccagac gtgcactcag ggtgtcccat
     8221 aaaccttcat caatggtttc tttcatgaaa gtttcgagcg cctcgcggac aactttggta
     8281 tatgccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc cgggttgagc
     8341 tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct
     8401 ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc catgtcattc
     8461 tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt cttacggtgc
     8521 actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg
     8581 ggaaaagcgc gcaaagcgcg gcaaaactga taaaaaaatc atcgttttcc attcggtaaa
     8641 aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt
     8701 aacacggcac acaggcgtaa tatctgacaa aactgcattt cgatttcttt cagtgcggaa
     8761 atcgtcatta cccgtgagtc tctttacatc atgaatatgc tggaaaaaat ccagtctcag
     8821 ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc ttcgcccgat
     8881 aacgcgatcc attcgagtat tgctgctatg gcactggaag ccaatgttag cgaaccgacg
     8941 gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa acttcatctg
     9001 gcacagagtc tggcgaatgg cactccctat gttaatcgca atgtcaatga agatgacagc
     9061 gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgcttga tcatgtccgt
     9121 cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa
     9181 atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat gaataagttc
     9241 tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg catgagttgt
     9301 atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag aacaaaaaat
     9361 ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct
     9421 gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt accggaagat
     9481 actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat agatgtgctg
     9541 gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa gcgggtcaag
     9601 gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga cgatcgctaa
     9661 aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt tatcgccatc
     9721 gcggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc agtcaacgga
     9781 gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag gcccagcaac
     9841 agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa
     9901 cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc gtgagattgc
     9961 cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca aaatccgtgt
    10021 atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc tgctcgacgc
    10081 caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc
    10141 tgctgacgtc gtgcctggtg acatcctgct gctggacgat ggtcgcgtcc agttaaaagt
    10201 actggaagtt cagggcatga aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa
    10261 caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga
    10321 caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg tctccttccc
    10381 acgctgtggc gaagatctga actatgcccg tcgcctggca cgcgatgcag gatgtgatgc
    10441 gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg caatggatga
    10501 catcatcctc gcctctgacg tggtaatggt tgcacgtggc gacctcggtg tggaaattgg
    10561 cgacccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg
    10621 agcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga tgccgacgcg
    10681 tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg tgatgctgtc
    10741 tgcagaaact gccgcagggc agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg
    10801 cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt
    10861 cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc acctgaaagg
    10921 cgttacggcg attattacca tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat
    10981 cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga acctgactgc
    11041 tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg tagcagctgc
    11101 cagcgaagcg gttaatctgc tgcgcgataa aggttacttg atgtctggtg acctggtgat
    11161 tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc gtattttaac
    11221 ggtagagtaa tctcacctgc cccggccgct gatggccggg gtatttagca acacataaat
    11281 taggactact tattcaatcc aatcataatc acagccccct gtaaaatgaa tacagggata
    11341 gcctgaatgg agatctaaat agcttgcaag acgtcgcata cccgcttcct ctgcaacact
    11401 atgtccaata actgccatcg gtaagccact atctttggag tattgtgtct gcacccatgt
    11461 tttaattgaa tcgttaatcg taatataata atcagcctga tattccatat attgtattgg
    11521 atcacaaaaa cagcctgccc caatgccaac agttctgact actct
//
LOCUS       DAJVTY010000132        11444 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVTY010000132 DAJVTY010000000
VERSION     DAJVTY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.132"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1073
                     /locus_tag="OQQ66_003131"
     CDS             <1..1073
                     /locus_tag="OQQ66_003131"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1905968.1"
                     /translation="PVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEK
                     VKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAV
                     QTGKSINPEFNIGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHML
                     NYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSN
                     PYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRID
                     YFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTL
                     ERIRKASFYWYRDLIANNGENI"
     gene            complement(1130..1348)
                     /gene="marB"
                     /locus_tag="OQQ66_003132"
     CDS             complement(1130..1348)
                     /gene="marB"
                     /locus_tag="OQQ66_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1905969.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1380..1763)
                     /gene="marA"
                     /locus_tag="OQQ66_003133"
     CDS             complement(1380..1763)
                     /gene="marA"
                     /locus_tag="OQQ66_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1905970.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(1783..2217)
                     /gene="marR"
                     /locus_tag="OQQ66_003134"
     CDS             complement(1783..2217)
                     /gene="marR"
                     /locus_tag="OQQ66_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1905971.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2429..3094
                     /gene="marC"
                     /locus_tag="OQQ66_003135"
     CDS             2429..3094
                     /gene="marC"
                     /locus_tag="OQQ66_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1905972.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3119..4309)
                     /gene="ydeA"
                     /locus_tag="OQQ66_003136"
     CDS             complement(3119..4309)
                     /gene="ydeA"
                     /locus_tag="OQQ66_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1905973.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4459..5573)
                     /gene="yneK"
                     /locus_tag="OQQ66_003137"
                     /pseudo
     CDS             complement(4459..5573)
                     /gene="yneK"
                     /locus_tag="OQQ66_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(5651..6532)
                     /locus_tag="OQQ66_003138"
     CDS             complement(5651..6532)
                     /locus_tag="OQQ66_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1905974.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            6633..8022
                     /gene="sad"
                     /locus_tag="OQQ66_003139"
                     /pseudo
     CDS             6633..8022
                     /gene="sad"
                     /locus_tag="OQQ66_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
     gene            8086..9012
                     /gene="glsB"
                     /locus_tag="OQQ66_003140"
     CDS             8086..9012
                     /gene="glsB"
                     /locus_tag="OQQ66_003140"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1905975.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            9012..9371
                     /locus_tag="OQQ66_003141"
     CDS             9012..9371
                     /locus_tag="OQQ66_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1905976.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            9510..10928
                     /locus_tag="OQQ66_003142"
     CDS             9510..10928
                     /locus_tag="OQQ66_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1905977.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVVMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            11155..11394
                     /locus_tag="OQQ66_003143"
                     /pseudo
     CDS             11155..11394
                     /locus_tag="OQQ66_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
BASE COUNT         2896 a         2598 c         2931 g         3019 t
ORIGIN      
        1 aacctgtggt gacgctttca cattttgaga tgccttatca tctggtgaca aaatatggtg
       61 gctggcgaaa ccgtaaactg atcgactttt ttatccgctt cgcatcaacg gtcttcacgc
      121 gctataaaga aaaagtaaag tactggatga cgtttaacga aatcaataat caggtgaatt
      181 tcagcgaaag cctgtgtcca tttactaatt ccggtatctt gtattcgcca gaggaagata
      241 tcaatgagcg cgaacaaata atgtaccagg cggtacatta cgagttagtt gccagtgccc
      301 tggcggtaca gactggaaaa tcgatcaatc ctgaatttaa cattggctgt atgatcgcca
      361 tgtatcccat ctatcctctg acgtgtgtac ccaacgatat gatgatggcc acgaaagcaa
      421 tgcatcgtcg ttactggttt actgatgttc atgctcgtgg atattatccg caacatatgc
      481 tgaattactt tgccaggaaa ggattcaacc tcgatatcac accagaagat aacgcgattc
      541 ttgccagtgg ttgtgtcgac tttatcggct ttagctacta catgtctttt acgacacaat
      601 tttcgcccga taacccgcaa ctggattatg ttgaaccacg agatttggtc agcaaccctt
      661 atatcgatac atccgaatgg ggatggcaaa ttgatccggc agggctacgt tattcactca
      721 actggttctg ggatcatttc cagttgccgc tgtttattgt cgaaaatgga tttggtgcgg
      781 ttgaccagag acaagctgac ggcacggtga acgatcacta tcgcattgat tactttgctt
      841 cccatattcg ggaaatgaaa aaagccgtag ttgaagatgg tgttgactta attggctaca
      901 ccccgtgggg ctgcattgac ctggtttctg ccggaacagg ggaaatgaaa aagcgctacg
      961 gaatgattta tgtcgataaa gacaacgaag ggaagggaac gctggaacgg atacgtaaag
     1021 cgtcgtttta ctggtatcgg gatctcatcg ccaacaatgg cgaaaatatt tgagacttga
     1081 ggaagatatt acggagatgt caggctctgt gatcagagcc tgacaataac tacatagcgt
     1141 gttgattata atagggcacg ccgagcgcat ccgacttatc actgccagta cccatgtgat
     1201 ttaaatcaaa cggtggttgt tcctgcgatg ggggaacaac cacgacattg gcacaagaag
     1261 taacaactgg ctgcgtggtt tgttccgcaa cgccctgcgc ggaaaagaga ataagcgcag
     1321 ctgctattgc ggatgaaagt ggtttcatga ttgcctcagt gacgttgtca cgttatcaac
     1381 tagctattgt aatgatttaa tggatgtaaa aagcgcgatt cgccctgcat attggtcatc
     1441 cggtatttat gtggcggaac atcaaagtaa tttttgaagg ttcgggtcag agtttgttgc
     1501 gactcgaagc catatcgttc tgccagatag agtatcggct cgttactttc cttcagcttt
     1561 tgcgcgattt ccgtcatctt acggctgcgg atgtattggc ctaatgaatg accggtttct
     1621 tttttaaaca tccgttgcag gtgccattta gagtaacctg aacgctctga cactttctcc
     1681 agtgacagtg gcgattccag gttgtcctcg atccagtcca aaatgctatg aatggtaata
     1741 gcgtcagtat tgcgtctgga catcgtcata cctctttttt gtttacggca ggactttctt
     1801 aagcaaatgc tcaagtgttg ccacttcgtc cgccgtcagg ttttttgtta attcttgatg
     1861 caggtcctgg ccaactaatt gatggcattg ttcgcatatt gccgcgccgc tggtggtaag
     1921 ttttaccagt acgccgcgct tatcattcgg gttcggcaac ctttcaaccc agcctttaca
     1981 gaccaggcga tccagcatac gggtcagtgc tccaaggtcg acagacaaca cttttttcag
     2041 ttcaaccgga gtaatacacg ccgcgcagcg gatagagcag agcaccttaa actgtgccgc
     2101 ggtaatatcc agcggagaca gatactcgtt aagcagacga tctttcttct gattaaccat
     2161 atggattaag cgacccaatg gaataatttc attgaacaga tcgctggtac ttttcacatt
     2221 agttgccctg gcaagtaatt agttgcaggg ggtaatattg cccaggcaag tataagtcaa
     2281 ataaatgaat tgaccgatgc cacgttttgc taaatcggtt caagttttgg tcaatgcgat
     2341 gcaaggttag ctaaagagtg acactttatc cttgtttacc ccttatactt ttcgctgata
     2401 acccagatac actggataac aaccaccaat gttagatttg tttaaagcaa ttggcctggg
     2461 gctggtggtg ttactgccgt tagctaaccc attaacaact gtcgcgttat ttcttggcct
     2521 ggcaggcaac atgaacagtg ccgaacgtaa tcgtcagtcg ttgatggcct cggtgtacgt
     2581 atttgccatc atgatggtgg cgtattacgc cgggcaactg gtgatggata catttggcat
     2641 ttcgattccc ggtctgcgaa ttgcaggcgg cttaatcgtc gcgtttatcg gttttcggat
     2701 gctctttccg caacagaaag cgattgattc accggaggcg aaaagcaagt cggaagagct
     2761 ggaagatgaa cccagtgcca atattgcctt tgtgccgcta gcaatgccaa gtaccgctgg
     2821 tccgggaacc atagcgatga ttattagctc ggcgtccaca gtgcgtcaga gttcaacctt
     2881 cgctgattgg gtactgatgg ttgcgccgcc gctgatcttt ttcctggtgg cggttatttt
     2941 gtggggaagc ctacgcagct ccggcgcaat tatgcggctg gtgggcaagg gtggcattga
     3001 agcgatctcc cgtctgatgg gattcctgct ggtatgtatg ggtgtgcagt ttattattaa
     3061 cggcatcctg gaaatcatta aaacgtacca ttaaaaaagg cccgaatggg cctttcaact
     3121 attgcgtctg ttcttcgagt gtcactggcc agcggcgaaa tataatgatt gaccatatta
     3181 acgcggcaaa agcaggcacc gcgcccacat aaccaatcat cgacattgac cagtgcaaac
     3241 tcacctgatt acctaccaac gcacccgccc cgattccaat attaaatatg ccggagaata
     3301 gcgccatcgc gacgtcggta gcatctggtg ccagcgccag cactttaacc tgcataccaa
     3361 gcccgatgat catcatcgcg atcccccaga aaatactcag caccccgagg tgtatttcac
     3421 tgttcgccgc aggtagcagc agtgccagac acaccagcaa cagcgcaatc gccgtactca
     3481 ccaacgcaga cgcatactga ttacccagtt taccgaaaat cacgctgcca ataatgcccg
     3541 caccaccgag taataacagt aatgccgtgg caaagttggc gctgaatccc gcaatgtttt
     3601 gcacaaaagg ctcgatatag ctgtatgccg tgtaatgggc ggtgacaacc accacagtta
     3661 acaaataaat gctcatcaat gccgggcggc ggaacaatag cgggaggctt ttcaatgaac
     3721 cggaatgctc actgggcagt aagggaagta acttaatcag gcacaaaagg gtgataagcg
     3781 ccccaatacc aatcgcgaag aaggtcattc gccaaccgaa atactgcccc acaatgcgcc
     3841 cgagaggtaa acctaagacc atcgccagtg ctgtaccggt ggcaattaaa ctcaaggcct
     3901 gtgcccgctt cccggccgga gccatacgga tcgccagaga cgccgtaatc gaccagaaaa
     3961 tcgcatgtgc aaaagccaca ccaatgcgac tgatcaccag aacggtaaag ctccacgata
     4021 aaaacgacag tacgtggctg gcaataaaca ccacaaacag gcagatcagt aatttgcgcc
     4081 gttcaacctg actggtcatt aacataaaag gcaatgacat tagcgctact acccatgcgt
     4141 aaatggtcaa catgatgccg acctgagcgg tttgcatgtg aaaactttgc gcaatgtcag
     4201 agagcaggcc aacagggaca aattcggtgg tgttgaagat gaaggcggcg actgccagcg
     4261 taacgacccg tagccacgcc actttgcggg aaacagtgtt tgttgtcata acagattatc
     4321 gggtgagttg cgaaaaagtg atgaaatgat cttaaaacgg ttaatgatgt gaatacaatg
     4381 catttgtgat ctggatcgcg ttttccgttt ctctgacgct gattctgttc cgcgtcagag
     4441 agtcggtaac atcacggttt aatgacgttc agggaggtct ggcgttttgg cggttgcggg
     4501 tataggtggt ctgacagggg agcgaggcct gtttttcacc aggtcttcgt gagtaacttt
     4561 tggcggcgtt tcatttttta caaacagttt gagggtcctg cgcgccatat gcttgataat
     4621 tttatcatca cttaatgatg ctggcatcaa cacgtcattc agatcacttt cattgggatt
     4681 cataaattgg tgatagtgca ataacatatt ggcgaaaaat aaaaaaaagt cctgaaaatg
     4741 aggagattct tgttctgcta aaagggataa aaagcagatg cagacttcgg gtagtacatg
     4801 tttgttagaa cctaatgaat ccatcaggct ttgcaataaa tcctctttta attcgccgtc
     4861 tgttatgtcg agaatggcgg tgatcaagtt aggatcttta ttgacgaacc atctgtgaac
     4921 ctcagagtta ctccataatc gggggagtat gacaaaatta ccgcgagaga taaaattcag
     4981 gacaagcaaa tcaaactggc gtaagtaatc tgcagcggta tggtgatccg gaggcagggg
     5041 ttcaatatgt ggtgtatgcg cttttttgat ggggacgcca aggtggatca ttggaaattg
     5101 ttcaataaaa tcattcggaa acagaaagag cacacgctcg acgccggtgt gaaatacgcg
     5161 ccccatagtg acaggctttg ggttgggagc gcaaaaatat taatttctcc atcatggcga
     5221 aaattatcta aataaatagg gtttaaattt attactacct gaaattcaat attttctttg
     5281 tgtcttccat tcttgacggg ataagtgagt ttaaactgaa ccttttcatt gtatagtgtg
     5341 tcgttacccc gaaaagaagt cctctgaata tatgataagt tatcaaggtt gttaaatatc
     5401 ttttttgtgg ctgagttggt gtttaaagac gtgcgccact cttcatcaca tattatggta
     5461 ctgagtgcat caataaaatt ctttaaaact tcttttacct gaggtgcaat attacgtacg
     5521 ggaaaatcat caggaagaaa tatgtaatta ctgatgctta ctggcgtaac catatttgcc
     5581 ccatatagtc ataagtcatc ggaacatcac tatatagggc atttgagctg caagaactat
     5641 caaaaatagc tcattggtaa ccctgttttg ccgagtcagg cacatccagt agttgaataa
     5701 atgcctcaag cggacgcgtt ttcgcaccac gacgccagac cagccaggtt gttaaccaac
     5761 gccattgctc agctaacggc cacgcttcaa cctggtgatg ccccggcata ctttccagca
     5821 tagagcgtgg aataagcgca atgcctgctc ctgcgaccac acaggccaac atcccgtgat
     5881 acgactccat ctcgtgaata gttcccggag cggcaccgtc agcatgaaac cagctctcga
     5941 agtggcgacg atacgaacaa ttggcgcgga aggcataaat gttactgccg ttaacctgac
     6001 tggcgcggat tactggcgca tgaccttgtg gcgtgacgat catcagctcc tcgcggtata
     6061 ccggtatccc gtcaatggca gtatggttaa tgggcccatc aataaatgcc gcattcagtt
     6121 ttccctccag cactccttcc agcatagtgc cggaagggcc ggtggaaagt gaaaactgaa
     6181 ttttgggata acgatggttg aattccgcca gcgtggccgg aatgcgcact gctgcggtgc
     6241 tttccagcga accaagagaa aataaacctt gcggctcatc gccagcgaca acgctccgcg
     6301 cttcatccac taacgcgaga atttgttggc tatagcgtaa aaagttatgc ccggcaggag
     6361 agagacgtaa acgctgattc tcgcgaataa acagatctac ccccagttct gtttccagct
     6421 ggcgtagacg gatagtgaga ttcgacggca cgcgaagcac tattgctgct gcctgggtta
     6481 tgctgccagc ttcggcaacc gcgttgaaca tctccagttg ggtcagatcc ataatattct
     6541 cgattcgtga ataagtggct taatattatt cattttaaag caagagtaaa tctgcgtatc
     6601 ttcataccat gactcataaa ggagataccc cgatgaccat tactccggca actcatgcaa
     6661 tttcgataaa tcctgccacg ggtgaacaac tttctgtgct gccgtgggct ggcgctaacg
     6721 atatcgaaaa cgcacttcag ctggcggcag caggctttcg cgactggcgc gagacaaata
     6781 tagattatcg tgctgaaaaa ctgcgtggta tcggtaaggc tctgcgcgcc cgtagcgaag
     6841 aaatggcgca aatgatcacc cgtgaaatgg gcaaaccaat caatcaggcg cgcgctgaag
     6901 tggcgaaatc ggcgaatttg tgtgactggt atgcagaaca tggtccggca atgctgaagg
     6961 cggaacctac gctggtggaa aatcagcagg cagttattga gtatcgaccg ttggggatga
     7021 ttctggcgat tatgccgtgg aactttccgt tatggcaggt gatgcgtggc gcggttccca
     7081 tcattcttgc aggtaacggc tacttactta aacatgcgcc gaatgtgatg ggctgtgctc
     7141 agctcattgc ccaggtgttt aaagatgcgg gaatcccgca aggcgtatat ggctggctga
     7201 atgccgacaa cgacggtgtc agtcaaatga ttaaagattc gcgcattgct gctgtcacgg
     7261 tgaccggaag tgttcgtgcg ggagcggcta ttggcgcaca ggctggagcg gcactgaaaa
     7321 aatgcgtact ggaactgggc ggttcggatc catttattgt gcttaacgat gccgatctgg
     7381 aactggcggt taaagcggcg gtagccggac gttatcagaa taccggacag gtttgtgcag
     7441 cggcaaaacg ctttattatc gaagagggaa ttgcttctgc atttaccgaa cgttttgtgg
     7501 cagctgcggc agccattgaa aatgggcgat ccccgtgatg aagagaacgc tctcggacca
     7561 atggctcgtt ttgatttacg tgatgagctg catcatcagg tggagaaaac cctggcgcag
     7621 ggtgcgcgtt tgttactggg cggggaaaag atggctgggg caggtaacta ctatccgcca
     7681 acggttctgg cgaatgttac cccagaaatg accgcgtttc gggaagaaat gtttggccct
     7741 gttgcggcaa tcaccgttgc gaaagatgca gaacatgcgc tggaactggc taatgatagt
     7801 gagttcggcc tttcagcgac catttttacc accgacgaaa cacaggccag acagatggcg
     7861 gcacgtctgg aatgcggtgg ggtgtttatc aatggttatt gtgccagcga cgcgcgagtg
     7921 gcctttggtg gcgtgaaaaa gagtggcttt ggtcgtgagc tttcccattt cggcttacac
     7981 gaattctgta atatccagac ggtgtggaaa gaccggatct gacgcgacat attcagctct
     8041 gatatactcg caggtctttt cagacctgcg gtccaggagt agaaagtggc agtcgccatg
     8101 gataatgcaa ttttagaaaa catcttgcgg caagtgcggc cgctcattgg tcagggtaaa
     8161 gtcgcggatt atattccggc gctggctaca gtagacggtt cccgattggg cattgctatc
     8221 tgtaccgttg acggacagct ttttcaggcc ggagacgcgc aagaacgttt ttccattcag
     8281 tctatttcca aagtgctgag tctcgttgtc gccatgcgtc attactccga agaggaaatc
     8341 tggcaacgcg tcggcaaaga tccgtctgga tcaccgttca attctttagt gcaactggaa
     8401 atggagcagg gtattccgcg taatccgttt attaatgctg gtgcgctggt ggtctgcgat
     8461 atgttgcaag ggcgattaag cgcaccacgg caacgtatgc tggaagtcgt gcgcggctta
     8521 agcggtgtgt ctgatatttc atacgatacg gtggtagcgc gttccgaatt tgaacattcc
     8581 gcgcgaaatg cggctatcgc ctggctgatg aagtcgtttg gcaatttcca tcatgacgtg
     8641 acaaccgttc tgcaaaacta ctttcattac tgcgctctga aaatgagctg tgtagagctg
     8701 gcccggacgt ttgtctttct ggctaatcag gggaaagcta ttcatattga tgaacctgtg
     8761 gtgacgccaa tgcaggcgcg gcaaattaac gcgctgatgg cgaccagtgg tatgtaccag
     8821 aacgcggggg agtttgcctg gcgggtgggg ctaccggcga aatctggcgt tggtggcggt
     8881 attgtggcga ttgttccgca tgaaatggcc atcgctgtct ggagtccgga actggatgat
     8941 gcaggtaact cgcttgcggg tatcgccgtc cttgaacaat tgacgaaaca gttagggcgt
     9001 tcggtttatt aatgcagtct ctcgatccac tcttcgcgcg tttatcccgt tcaaaatttc
     9061 gctctcgctt tcgtctgggc atgaaagagc gtcagtattg cctggagaaa ggcgcgccag
     9121 tcatcgaaca acatgcggcg gattttgtcg ctaaacgtct tgctccggca ttaccggcta
     9181 atgacggtaa gcaaacaccc atgcgcgggc atccggtgtt tatcgctcag cacgccaccg
     9241 cgacctgctg tcgcggttgt ctggctaagt ggcataacat tccgcagggt gtttcgttaa
     9301 gcgaagaaca acaacgttat atcgtggcgg taatctatca ctggctggtt gtacagatga
     9361 atcagccatg agaattgcgg aaaacgataa aatagcattc ccagggtacc tttatttgcc
     9421 gccgctgtta gcgaacatgc tatcctttat gagaatttac ttattacgtt gagataattg
     9481 ccacccgcaa ttgtatggac agataattaa tgcatgtaca ccccatttca actttccgtt
     9541 tgttccagga aggtcatctg ctacgtaata gcatcgctat ttttgcgcta accacgctgt
     9601 tctattttat tgttgcagag ttacggctgg ttcacgaact ttctcttttc tggccgctga
     9661 atggcgtaat ggcgggggtg tttgcccgct atgtctggct taatcgactg cattactatg
     9721 cgatcagtta tgtggtgatg ctggtttatg atgccataac caccgaatgg ggactggttt
     9781 cactggttat caatttctcc aatatgatgt ttattgttac cgtcgcctta ctggtcgtgc
     9841 gggataagcg tctggggaaa aataagtatg agccagtaag tgccttacgg ctatttaatt
     9901 actgtctgat tgccgcatta ttatgcgcta ttgtcggggc gattggttcg gtcagtattg
     9961 atagtctgga tttctggcct ttgcttgcgg actggttcag tgagcaattc tcaacgggcg
    10021 tgttgatcgt gccttgtatg ctgacgttgg ctattcctgg agtgctgccg cgctttaaag
    10081 cagaacagat gatgcccgcc atcgcgctta ttgtgtcggt tattgcctcg gtagtcattg
    10141 gcggagcggg gagtctggcg ttcccgctgc ctgcattaat ctggtgtgca gtgcgctata
    10201 cgccgcaggt aacatgtctg ttgacctttg tcaccggtgc ggtggaaatc gtactggtgg
    10261 caaattcggt gattgatatc tcggtcggtt cgccgttctc cattccggaa atgttctccg
    10321 cacgtctcgg tattgccacg atggcgatat gcccaattat ggtttctttt agcgtggcag
    10381 cgatcaattc gctaatgaag caagttgcgc tgcgagccga ctttgatttt ctgactcagg
    10441 tttactcacg gtccggtctt tatgaggcgc tgaaaagtcc atcgctgaaa cagacacaac
    10501 atctgacggt catgctgctt gatatcgact atttcaaaag cattaacgat aactatggac
    10561 atgaatgtgg cgataaagtg ttaagcgtgt ttgcccggca tattcagaag attgtcggtg
    10621 ataaggggct ggtggcgcgc atgggcggcg aagaatttgc tgttgcagtg ccgtcggtga
    10681 atcctgtcga tggtctgcta atggcggaaa aaatccgtaa aggcgttgaa ctgcaaccat
    10741 tcacctggca acaaaaaacg ctttatctca cggtaagtat tggcgtcggt agtggtcgcg
    10801 catcgtaccg aacgctgacc gatgatttta ataaattgat ggtcgaagcc gatacgtgtc
    10861 tgtatcgctc gaagaaagat gggcgcaacc gtaccagcac tatgcgttac ggtgaggaag
    10921 ttgtctgaca tctttattca ttccgcgcga tttgaatgca atgtgttcta ccactttatg
    10981 atccaggaaa aggcgtattt cctcgattaa accatgatcc gcgccacact tttctcataa
    11041 aactgctcat taatcatcag gagttgatca aaacaggggt tcggcgtatt aaattttgct
    11101 aacctgtcgt accaattcaa gcgggttgta tgactaatca gaagggaacc cattgtgaaa
    11161 acactaaatc gtcgcgatat tcccggtgca cagtatccag aacgtatcat tcagtttggt
    11221 gaaggtaact tcctgcgcgc ctttgttgac tggcaaatcg atctcctgaa tgagcacacc
    11281 gatctgaatt ctggcgtggt cgttgttcgt ccgattgaaa cttcattccc gccgtcactg
    11341 agcacgcagg atggtctgta caccaccatt atccgtggcc tgaatgagaa ggggtgaagt
    11401 ggtcaacaaa aactggccac cgagttagag tttttccagt atcg
//
LOCUS       DAJVTY010000133        11227 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVTY010000133 DAJVTY010000000
VERSION     DAJVTY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.133"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            63..662
                     /locus_tag="OQQ66_003144"
     CDS             63..662
                     /locus_tag="OQQ66_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1905978.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
     gene            complement(691..1179)
                     /locus_tag="OQQ66_003145"
     CDS             complement(691..1179)
                     /locus_tag="OQQ66_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1905979.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1192..3999)
                     /locus_tag="OQQ66_003146"
     CDS             complement(1192..3999)
                     /locus_tag="OQQ66_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1905980.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4037..4141)
                     /locus_tag="OQQ66_003147"
     CDS             complement(4037..4141)
                     /locus_tag="OQQ66_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCS1905981.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4150..4524)
                     /locus_tag="OQQ66_003148"
     CDS             complement(4150..4524)
                     /locus_tag="OQQ66_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1905982.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4580..5090)
                     /locus_tag="OQQ66_003149"
                     /pseudo
     CDS             complement(4580..5090)
                     /locus_tag="OQQ66_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5090..6274)
                     /locus_tag="OQQ66_003150"
     CDS             complement(5090..6274)
                     /locus_tag="OQQ66_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCS1905983.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6432..7541
                     /locus_tag="OQQ66_003151"
                     /pseudo
     CDS             6432..7541
                     /locus_tag="OQQ66_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7790..10909
                     /locus_tag="OQQ66_003152"
     CDS             7790..10909
                     /locus_tag="OQQ66_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1905984.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSKKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            11045..>11227
                     /locus_tag="OQQ66_003153"
     CDS             11045..>11227
                     /locus_tag="OQQ66_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1905985.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSS"
BASE COUNT         3074 a         2814 c         2512 g         2827 t
ORIGIN      
        1 ttaaataatg gccgtttctg gcctgcatgg catgatttgc gcttttggac gggagattca
       61 gtgtgctgat tggctatgta agggtatcaa caaatgacca gaatacagac ctgcaacgaa
      121 acgctcttgt ttgtgcagga tgtgaacaaa tatttgaaga taaattaagc gggacaaaga
      181 cagaccgacc gggattaaaa cgcgctttaa agcgccttca aaaaggtgac acgctggttg
      241 tctggaaact ggatcgcctc gggcgaagca tgaaacatct gatttctctc gtcggggaac
      301 tacgggagcg agggattaat tttcgcagtc tgaccgacag catagacaca tcttctccaa
      361 tggggcgttt tttcttccac gtgatgggtg ccctggctga aatggaacgt gaattaattg
      421 ttgaacgtac actggccggg ctggcggcag cgcgcgcaag ggggcgcaca ggcggacgtc
      481 gaccgaagct gacaaaagaa cagcatgagc aaatagcaag gctaatcaaa aacgggcacg
      541 acagaaaaca actggcaata atttacggca ttggcacatc gacgatttat cgttatcacc
      601 ctgtaggcga tatacagact gaagaaacaa ccgggcagac tcaggaaaat gaaaaccgct
      661 aatctgacca ttagcggttt ttgtgttaaa tcagaacagc cctttaactg aactggccgc
      721 gctgttaaga gatgatgtca ccttatcttt gaagccggac agcatatcac tgaacgatga
      781 ggattgcagg cgctcccgca aatcctcatc acagcgttca agagtcagtg aaaattctat
      841 ctttttcgcc ttaccgtagc gatcaaactc ggagcgggtc gtattcgttt cagtcaggac
      901 atacatgccg taaatctgcc cgacgccatc aatcaaaggc cagggtcgtc ctgtatacgc
      961 ctgcgtagtc agcagcgaca gcgacacttc gccacctgta atttcaggat aaagcacacc
     1021 agaaagaacg atgcgatcat cacctgcacc gatatactgc cagcttgctg aacggttaac
     1081 gcgttcattt ttcacatgcc gccagctttt gttttgctgt aactgctgat gcggcagcgt
     1141 gcgcagctca aaaacaaaca tgccgtagat catcatcatg gccatgactc ctcaatcttt
     1201 atcgtaaaaa cagccacgcc cggcacgggc gcgccgttcc atttctgccc tgaccatttc
     1261 accgaccagt ttcgccagtt cgcggggatt ctgcgtaaca acgttatgca gatgaacatg
     1321 aatttcacca ccaaatccga aggcaacagg ctcccggtta cgggaagtta caggaactga
     1381 tgccactgga gatcgtatag cctccgccac cgggcgggag ctggccgcaa caacagggac
     1441 cagcgccgga ggcagcggag ccggaaccac gggtgtgata ttaattgcgg gggcaggctt
     1501 actgacctgc gcaatcttcc gctcctgcca ctccccacga acagcaagtg ctcggggcag
     1561 gtttttaaag acaatatcgc cggggccaat gcgttttttc gtctcctcaa ccagcttacc
     1621 tgtgttatca gcaattttgc tgagtctgcg cagcgtaccg gtattgctgt ctgtgagcgg
     1681 tttattgtct ttggggttat cacctccggt gccattgcca ttttccacag gcttcggcgg
     1741 attgattttc gccagatccc cctgaagcaa ggcaaccttg tcctgaagaa tggccgcacg
     1801 ctgtgcgtct tcgattttct tgcgcgccct ttccgcttca tccggaagca caccaagctt
     1861 ttcaagtatc cacgccagcg tatccagcaa catttttgca ggtgtcagaa caagttgtaa
     1921 cgcaccgcca agaacgttac cgaatatctc gccagcactg gtacatttat ccagagtttc
     1981 cttgctggac tccatcggtg acagcagcga tttaaaccag ttaaacacct ggctgatccc
     2041 actcccgatt gcgtcaaaaa caggaccaaa ccgttcaaag gtttcgcgca acggggtcag
     2101 cctttccata atcccgctga acaccccggc aaaaaatgcc ctgatgggat cccagtattt
     2161 ccagataaga acggcagctc cggcaagcgc agccacgata agaccaaccg gactgaacaa
     2221 cgccccgata gcgcctccca gtaaagaaac ggaacccgtc accattcccc atagtgctgg
     2281 cagaaccctg acagcattca ttgatccggt caggagggaa aaaccaagac gcagttttgc
     2341 cagcgggcca gcaagcacac caatagccag cgacaacgag ccaaccgttg cagtcattgc
     2401 cagcaatgca ccgcctgcaa tcagtagctg gcgcgtcagt gcgggatggg cctgcgccag
     2461 cgccgtcacc tttgatacca cacgcgtgag ccactgcgtg acagaacgca gcggaccgtc
     2521 aatcagatct gcaatgcgga tgcgcaaccc ttcccatgca ctgctgagtg atttcagatc
     2581 gccgtcaagg ttgttggcca taacctttgc cgtgcgttca gcctcaccgc gcgcgccttc
     2641 aagttctttt ctcagtttgg gtaaggagcc gtcacctgcc gcatcaacga gggccataaa
     2701 tgatgtgaaa gcctcttctc cggcaatgtc cttaaagaac gatacccggt caacttcccc
     2761 gtatttgcgg gtagctttat aaaggtcagc cagcacatcc tccatcgggc gcattttgcc
     2821 cccggcatcc gagacagaca cgccaagctc tttcagcgcc tctgctgccg cctttggcgg
     2881 tgatgccaga cgagccaggc tggcacgcat tgccgtacca gcatcactcc ccctgatacc
     2941 catattcgcc agcacgcccg ccatcgctgc ggcctgctcc agagatattc ccagcttacc
     3001 cgccaccgga cctgcatatt tcatggtttc acccagtgcg cgaaggtcag tgttggtacg
     3061 ggtaaacgct gcggtgagtg tgtcgccgac ccggtccatc tggtcagcag aaagaccgaa
     3121 ctgcgtcaga atatttgagc caatatccgc cgtctcaccg aggtccatac cgccagccgt
     3181 tgccatgctc agtacgccag ggagcgcagc ctgaatggcc tgtggtgtaa agccagccat
     3241 tgcaagaaat gcctgtccac tggcggcatc gcctgcggtg aactgcgttt cagagccaag
     3301 ttttaacgcc tgctcacgca gcgccttaaa ctgcgggctg ttctggtcga ttcgcgtcag
     3361 tgcctgaacg cgggacatct ctttgccgaa cccgatcgca ggctgcaaaa aacgcccggc
     3421 agcatagccg cccgccgctg ccgcaccaat tgccagcgca ccacctgttt tcagttttcc
     3481 cgctgtttcc tgcgcgcgcg aataccgctc acgcgcccgt gttacacgcg caagcgcctg
     3541 ccgttcgcgt tcaagctggt tgttgtactg ttcggtgcgt ctgatggcct gctggatggt
     3601 gttatcgctg cctgtcaggg aaatgccgtg gcgtttcagc tctccgccaa gctcccgcat
     3661 tttctgaatt tcccgtgtgc gcgattcatt caggcgttca agccgggtgc ttaactgctg
     3721 catcagcttt tgttgttttt cgctgagcac tgtacccgtg cgttgtaact gattaagggc
     3781 gttaagctgg cgtcgtgctt tcacgatgcc agcatccgct ttactgacag cgtcacgggc
     3841 gcgctcaaat gatcgcgcct gacgctcgag atttttgatc gccccctgcg ttcgctggat
     3901 ggagtcacca aactgcccca tcaggcggcg ggcgttttcg gcaggccggg tcagcctgtc
     3961 aacggcgctg aaagcgaccc ggatatcaag agtcttcatt gtctgcattc ccgctacgaa
     4021 gtgccgcccg ctcacgtcag ctaaccactt cgccgggcgt catcatgaag atttcggcgg
     4081 gcgaccagtt aaaaatggcg gcaatatccg ccaccagatc ttcgatgtgc tcaaagcaca
     4141 ccagggtgat tacgctgccg tctcctgcac gctcttcgcg ccagagtctg gctcgctcat
     4201 aaaatttaca gccacagcgc acaactgaat aaaatcgcgt gacgacattt ttttaatcat
     4261 cacttcatcc agtcgtggcg aggtcacgcg aggcaacagc gtgaacatgg tatccgcttt
     4321 cagattcagc acatcagaca gcgacagacc acgcagggat ccagcctgct caatagcccc
     4381 ggtgatctcc acatacgtga ttttttcgcc accacgctca attggtcggg tcagttttac
     4441 gccacgttcg acagccatat cctcaactgc cgtcacatta tccgccacgg tgttattccg
     4501 ggtttcagta tcgatgtctt tcatctgttg tctccttttc agtcagaggc gacgcactgc
     4561 gccgccagca tattacttat cagccaagcc caagcgcgga acggatgcga tcgggcacaa
     4621 tgtccttgcc gtccttccgg taaatgaagt tcagcaggtc aatctcccac aacgggcgat
     4681 cgttaacact cagcttgtag taggtgtttt aatggcgtaa gtgtgtgatg tggcttcgcc
     4741 ctgtttggct tcccccatat caatttccgt cacacgtccg cgcatttcga cttcatacag
     4801 gtcgctttct gcatcggtgt agtattcacc cgcaaaacgc agcagcgtgc cgtcaatcgt
     4861 gccgccatac ttaaggaaca gctcacgaac tgcgccccca tgacaaagct cgcatcaagc
     4921 gcggagtcgt ccagaccgag atcaatactt accgcaccca tcatgccacc accccggtag
     4981 ctgtcggttt tgcgcgtcag cttaggcaga gtgacggacg tcacattacc cacttcgttt
     5041 tcaccatcca caaacagcgt aaaaaagcga agatgttttg gcacagccat caggcacctc
     5101 ccagcaccgc aaatgcgggt tcaaagtatt catcagtaaa cgtctggtaa agctccatgt
     5161 cttccagtgg cggaacgggc gtatatttgt agcgaatacg cacacgtccc tgacgtaaat
     5221 ccgtggtgct gttatccacc acgtcatacc agcactccgc gccaatcagt ttcccggcag
     5281 tcaccagtga atccagtttt gccctgatgg cactgataac atccttcaca ttcgcaggcg
     5341 tcagtggact gtcgatggtt tcaaactgcg cttccgcaat tgaatcagcc agcacctgtg
     5401 cggttcgggt atacacctca aagatgtagg cgttcgtttc cggtgtgcgg ttaccccaga
     5461 agcggaaccc gttgcgacga ataatggtcg tgatttcttt gttgttgagg ctgttggcat
     5521 cgctgtcttc ggcctgcaac gaccagaaca catgcctcga catccccagc acatttttaa
     5581 ccggaacgtt ggacagcgat ttgtgccatc cctgttcatg gtcaatgtac gcacgaaggc
     5641 cgcacgcata ggcaggcgcg gggaacgttt cgtttttgcc atttttcggg ttgtaggcga
     5701 tgaagtccgg ccataagagc atcacctcac gttcgttgaa tttctggcgg taggtaattg
     5761 cctcggccat cgtgttacag ccatgacatg aggcatacac aaacgcgcgc agtttatctg
     5821 caatcacgca cagggatttt gttaccgcct ccgtgtccag ctccggcgcg gccagaatac
     5881 gcggacggta tccgatgctt tcatcctgct ctgcaacaag cagcgcatac atccccgtat
     5941 agctgccgtc atcctcagaa ccaccgataa ccagttgatc ctgcgtcttt ccgtcttctt
     6001 ctttgtgttc agccacgcga acgacgatca cctttgtgct cacctggtct gcgatggcct
     6061 taagcgcacg ataaagcgtc cccgttgtcc cgcattttcc cagcacgtca ttgacgcggg
     6121 tcagcagtgt gggcttgttc agcgggaaca gcttcgcgtc cgcatcatcc gccgttgcca
     6181 cgataccgat aacgctggaa tcaacatcgt taatcgctgt taccaggtcg gtattctccg
     6241 taacacgggc accatgaaaa cgagtttcac tcatagcttc agccccttgt atccgttaaa
     6301 tgattcagca acaatcatca cccaccacgc gcgtaatctc acccctgcgc cgttctcccg
     6361 ccacggcgac aacaaaaagc agtaaccccc tccgcacgca catgcgacca tgccgcacag
     6421 ggagggagca gatgaccgac accaccatgc aattgctcag tcagggcacg gcccccgtga
     6481 aaatgccgga ttttgatatt ctctcggagg gtaaaacgct gtcaggcgtg gcggagcgcc
     6541 tgatgagcct gtaactgacc gacaaccggg gatttgaggc ggaccagctc accatcacgc
     6601 tggatgatgc ggatggtcag ttgcagctac cgccacgggg cgcgcgtctg acggttctca
     6661 ttggctggaa aggggaaccg ctgacagaaa aaggcactta cattgttgat gaaatcgctc
     6721 acgaaggacc gccggacagg ctgactgttt cagccagaag cgcagatttt cgggatgaat
     6781 ttaacgttaa acgtgaggtg tcctggcatg atgtgaccgt tgagcgtgtg gtatccgcca
     6841 tcactcatcg gtacggtctg aaaccgcaaa tcagcgaaat gctgatggat atcgaaatcg
     6901 accacgccga ccagaccgaa gaaagcgaca tgtcctttct tacgcgcatg gcggaaatgc
     6961 tgggagcaat cgccacggta aaaagcggca atctgttatt catcatgcca ggtggtggcg
     7021 tgaacgcaca ggggcagccg ttgccctcgt tcgccattac acgcagcagc ggcgatcgcc
     7081 atcagttccg cattgctgac cgcgaggcgt atacgggggt acgcgcctac tggcttgatc
     7141 ttaattacgg gaaaaagaaa aaagtcagcg tgaaacgccg caaaccgcca aaacccaaaa
     7201 aggagaaaag cagcagtcgg gaaggcgact atatggaagg tgcggaaggc aacgtgtttg
     7261 tgttacgcaa gacttatcag aacgagcagg cagcaagacg cgcagcggcg gcaaagtggc
     7321 agcagctaca acgcggagcc gcatcattct ccatcacgct ggcgcgtggt cgtgcagaac
     7381 tctatcccga aatgcatggc acagtgacag gctttaaaag cgagattgat tatcaggact
     7441 ggactattgc aaaagccgag cacaccattg ataacagcgg ctttaccacg cagcttgaac
     7501 ttgaagcaaa aatcccggaa tggatagcag aaacagaata aatctcttaa aaatattatt
     7561 tttgagatgt gcttctacac tgacagtaag aacaaaagat aatgtatcag cccactagat
     7621 gtcagaggtt ggggcatcaa ccagatgcta acaataatac ggatgttttt cccacatgac
     7681 agaggaagtc aagtataatc ttcacagtcc atcccgtcca agggaatatg actcaatagt
     7741 aaaagttcca gtttagttag tagacccaca ctttatagaa gagtaattca tgattggaca
     7801 atttttaagt gcaacagaga ttttagcaaa gaactatgtt cgtaataaaa tggttaaaaa
     7861 tccattttat tcgaatttaa aatggaattt tattgaaaaa aacatcataa gattaacatc
     7921 ctcaccggtt aaatcagtac tttgcatatc agctttttca tttgtactac tttatgttgg
     7981 atatttaaac gaattattta taaaaaataa tcttttacat tatttcccgt ttaggcattc
     8041 attaaccgaa tggcaaacaa ccattttaag tggccaattg acaattattg gtattgtata
     8101 cccccttgtt ataggtttag taagtgtctt attccaaaaa aaggcagata gaaaaatagc
     8161 ccaaactgct tatcagcgtt actcaggatt catgcttgct ggactcagcg ggcttttctt
     8221 atcaggattt atacttctca gtgtactaat taaaactgtc tttggaagtt atctttatgg
     8281 tatagcttgt ctaatcagca tattatggct tttgataaac atagttcttt ctatttggtt
     8341 ctttatcgtg agtcttgaaa ttcttgatga tgtaaaacgg caaataatta taaagcgata
     8401 cattgccttc gaaatagtaa tgccccatat ttgtaacaaa atctcagcca agcttaggtt
     8461 ataccctatc tatcaaaaac ataactattc aaatttagaa atcacgcaag ccgattacaa
     8521 aggagaatac atatctgtgg ctagcagtta ttctaaggaa gatgagttaa gtttatatca
     8581 ccgtcctttt caacttactc ttaatcttat taattaccaa ctaaaaaaga agaatcattt
     8641 tgcgtcattt gtcattggtg ataatcgtac aaaagaaaca gaatccacgg gtaagatact
     8701 ttttagcgtt aaaaatatca aaccagacag cttattaata aaaatactta agcaatgttt
     8761 ttatagggct ccgattaaag gaggagattt ttccgtaagt cttacaatgc aggctataac
     8821 agcagatacg tatatgtatc tgcgggattc tgatctcatt agttttgaca atgctatctc
     8881 tgcattaatt aataacttta ataatctctg cgatttgtat ttttttcaag atgataatac
     8941 caacaataac ttcttactga tcactacaga attatttgaa cggagttttc aatatgagtt
     9001 ttcagatgaa gtttataaaa tatcaaataa ttccatggat aaaattaatc tttctgaaag
     9061 attttttgaa ttatgtcttt ggagcggagt gcgtattatc aataatagaa aacatcttat
     9121 cagcaatgaa ctttgtatct atatgggaat tactcgttca caatggtcaa tattaacaga
     9181 atggttccga aacaaccaat cactcttaaa tgcctcgctt cgttcacgtt ataatcgtat
     9241 attaagaacc tatatcactg tttgggagca atatcaagaa agcataaact ttcgtttctg
     9301 caatacagag aattcagatt tatttgaatt attttgcaaa acgcaactac aagaacttcc
     9361 atcgatgatt attgatgcaa cacagactcg cgatccttct actattgata cagccgtcga
     9421 tcttattaac cgatggcagc actcaatgaa tattgacagc cattcggtag aaaaatacag
     9481 ttacaaaggc cagcttttta acccgggttt tttcatatcc aaaaaactaa acttcaattc
     9541 agacagagag tggttcaata ttgccatcat aaatgcgttg actgacatgc gtatatgcac
     9601 atgtctttat ctgacatcaa gaatcaatac ttctgacaaa ctaatgacac attacattaa
     9661 acttatttta gagggtaaac ttattgacca aaccggtgga tatgaaactc ccactgaaga
     9721 aattgataat gcaagccaac taatcaagat attaattaga atctgcctgt ggacatggtc
     9781 tgaaaatatg gagcacaatg ggtggatgaa tagtttagca aggcgtttac gtgactatga
     9841 taaaacagac atggtgatgg gcagagttta cagtaatgtt tttgactgtg gatttattga
     9901 tatggaacaa tcatgggttc agctattatt aattttttct aaaaagaacg atagcgtatc
     9961 aaaagaaata aaagaagcaa tagaaaacaa ctatataaca tatcgtgaga agcaacgact
    10021 cattggagta ttatcaaaaa tttgtaactc catcgaatac actaaaatca agctgacttt
    10081 aacattggac gatctccaaa caaaaaaaga aaacttaaga aagttgcttc aagagcatat
    10141 taacatgcta aagaaagatc ttgatatgag acttcaggat gctgccatag atgtacatcg
    10201 actagatagc actgcacgta aaacatcaga acatcttcgt aaacgcatca agaaaacgct
    10261 ccctctttca cttttcaaat ctattgattt taagcaggct agcgattgtt tcacaaaaca
    10321 taagatatct attaagatag ataaagaacc gtatgccgaa ggaatagaat ccatccctta
    10381 tatcaatgaa ggtgatattc aagcagactt aattctcaag gacatccaga gaataatact
    10441 ttcaaattta tttagcactg gctgttcgca acataccgta atagaagatt tcaatatgtt
    10501 gattgatcac ataaaatcat ctgctgactt ggctggcaaa ctggttcttg tgatgagtaa
    10561 agagatcttt cagcaatata atcgtatgct tttcgataat cccaacctca gagaactaat
    10621 gagaaaaaat gatgatggct ctatgaacat aactactgaa tccggtaccc gtaaggttta
    10681 cttcttacca tttgttaatc aaccattttc tcttgtggta aaggataact acttcactaa
    10741 attaattatt agagaatatg ataataataa gttagtaaat gtcacttcag agaatatcaa
    10801 aagtgactca gataaattca agctaactct taactatgag ttgaatatag tatttgaagg
    10861 aaacgctgat ttaaaaatag ctcactcgca aagagtgaca tcagaataat atctttgacg
    10921 gcgctcctta gagtgccgtt cgtttattaa ctaatgctaa aaatcattat atagagaaag
    10981 tcatgtttaa atcttagaga atacaaccta gaatagtgta agcactacaa taagagaggt
    11041 cgctatgttc cgttgtccgc tttgtggcgc atctgcccgt atccgcacca gtcgtccgga
    11101 aaatgattca aacaccgtga gacaaaagta ttatcagtgt aacaatctag aatgcggcgt
    11161 atgtttctca acactggaag ccttccacaa attcacttca aagcacgcct cccccattca
    11221 ctcatca
//
LOCUS       DAJVTY010000134        11117 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVTY010000134 DAJVTY010000000
VERSION     DAJVTY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.134"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..362
                     /locus_tag="OQQ66_003154"
     CDS             <1..362
                     /locus_tag="OQQ66_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS1905986.1"
                     /translation="DILPITCFVGEDWQQDFQPLDGGPVQSLSDSYSDYSVLLLIAAE
                     SEISVPEWLAHSEGTTVILIAEHNARLLTGPAGGAVALIHYLNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            496..1040
                     /gene="cobU"
                     /locus_tag="OQQ66_003155"
                     /pseudo
     CDS             496..1040
                     /gene="cobU"
                     /locus_tag="OQQ66_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1037..1780
                     /gene="cobS"
                     /locus_tag="OQQ66_003156"
     CDS             1037..1780
                     /gene="cobS"
                     /locus_tag="OQQ66_003156"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1905987.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            1792..2871
                     /gene="cobT"
                     /locus_tag="OQQ66_003157"
     CDS             1792..2871
                     /gene="cobT"
                     /locus_tag="OQQ66_003157"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1905988.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            2933..3868
                     /gene="ldtA"
                     /locus_tag="OQQ66_003158"
     CDS             2933..3868
                     /gene="ldtA"
                     /locus_tag="OQQ66_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1905989.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(3924..3999)
                     /locus_tag="OQQ66_003159"
     tRNA            complement(3924..3999)
                     /locus_tag="OQQ66_003159"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3964..3966),aa:Asn,seq:gtt)
     gene            4326..4697
                     /locus_tag="OQQ66_003160"
                     /pseudo
     CDS             4326..4697
                     /locus_tag="OQQ66_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4696..5130
                     /locus_tag="OQQ66_003161"
                     /pseudo
     CDS             4696..5130
                     /locus_tag="OQQ66_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5473..6189
                     /locus_tag="OQQ66_003162"
     CDS             5473..6189
                     /locus_tag="OQQ66_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1905990.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6645..6720)
                     /locus_tag="OQQ66_003163"
     tRNA            complement(6645..6720)
                     /locus_tag="OQQ66_003163"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6685..6687),aa:Asn,seq:gtt)
     gene            complement(6821..8464)
                     /gene="yeeO"
                     /locus_tag="OQQ66_003164"
     CDS             complement(6821..8464)
                     /gene="yeeO"
                     /locus_tag="OQQ66_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS1905991.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8469..8544
                     /locus_tag="OQQ66_003165"
     tRNA            8469..8544
                     /locus_tag="OQQ66_003165"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8502..8504,aa:Asn,seq:gtt)
     gene            8706..9968
                     /locus_tag="OQQ66_003166"
     CDS             8706..9968
                     /locus_tag="OQQ66_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1905992.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10687..>11117)
                     /locus_tag="OQQ66_003167"
     CDS             complement(10687..>11117)
                     /locus_tag="OQQ66_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCS1905993.1"
                     /translation="VQSAQSLRTWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIR
                     KALDNALVVAEDRHGRLIDKPDLKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIR
                     HYLAQGFSEKEALAMTAMDLGHGDGRGRYVAQVYGRKDTD"
BASE COUNT         2955 a         2596 c         2705 g         2861 t
ORIGIN      
        1 ctgatatatt gccaataacc tgttttgttg gtgaggactg gcagcaagat ttccagcctc
       61 ttgatggtgg gcctgtgcaa tcgttatcag attcgtatag cgattattca gtgctgctat
      121 tgatagcagc tgaaagcgag atatccgtac cagaatggct cgctcattct gagggaacga
      181 cggtaatttt gattgcagaa cacaatgccc gtttgctgac tggtccggct ggtggggctg
      241 tagcattaat ccattatctg aatgaaatct gtcattccag agaaaccgct gtcaatgacg
      301 cattttgtcg gacgttaaca cgaggaagtc tggctattcc gcaatgccat cttctttcgt
      361 aatggattga gaatgctgga tttttaatta tattacgcgc agtattaatc gcgtaaatgt
      421 tttgcggtca gcaacggtgg gggatccatt gttctgttca atgaatacca tcatacaatt
      481 gaatcaggag tcggtatgat gatttcggtg acggggggcg tacggagcgg gaagagtcgc
      541 cacgcagagg cgcttattgg ggactcttca caggttctgt atacgccacc tcgcaaatcc
      601 ttgatgatga gatggctgca cggatagaac atcatcggca aggccgcccg acgcactggc
      661 acacagtgga gcgctggcaa catcttgatg aattaattca tgcagacatt aacccgcatg
      721 aggctgtatt gctggaatgc gttaccacca tggtgactaa tctgttgttt gattatggcg
      781 gcgataaaga ccctgatgaa tgggattatc aggcgatgga acaggcgatt aatgctgaga
      841 ttcagtcgtt gattgctgcc tgccaacgtt gtcccgcaaa ggttgtatta gtgactaacg
      901 aagtgggaat ggggattgtg ccggagagtc gtctggcacg acattttcgt gatattgccg
      961 ggcgggtaaa tcagcagctg gccgctgcgg caaatgaagt atggttggtg gtttcgggta
     1021 ttggagtaaa aatcaaatga gtaaattatt ttgggcgatg ctctctttta ttacgcgtct
     1081 gcctgtaccg cgtcgctggt cccagggact ggatttcgag cattattctc gcggtattat
     1141 tacttttcct ttaattggat tattgcttgg cgcgattagc gggctggtct tcatggtgct
     1201 gcaggcatgg tgtggcgtac cactggcggc actgtttagc gtacttgtgc tggcgctgat
     1261 gaccggggga ttccacctgg acggcctcgc cgatacctgt gatggcgtat tttctgcacg
     1321 tagccgcgat cgcatgttgg aaatcatgcg tgatagccgt ttaggcaccc acggcggtct
     1381 ggcattgatt tttgttgtac tggcaaagat tctggtatta agcgagttgg ctctgcgtgg
     1441 cgaaccgatc cttgcattgc tggcggcagc atgtgcggtc agccgcggga ctgccgcatt
     1501 attgatgtat cgtcatcgtt atgcccggga agaaggactt ggcaatgtgt tcattggcaa
     1561 aattgatggg cgacaaacct gcgtcacact cggcttagcc gctatttttg ctgccgtatt
     1621 attgccgggt atgcatggtg tggctgctat ggtggtgacg atggtggcga ttttcatcct
     1681 cggtcagctt ctcaaacgta cgctgggcgg gcaaaccggc gatacgctgg gagctgctat
     1741 agagcttggt gaactggtct tcctgctggc actgctataa agagacgtga cattcaaaca
     1801 cttgccgatt tactgaatac gatccctgct atcgatcctg ccgctatgtc gcgtgcacaa
     1861 cggcatatcg acgggttact caaacctgtt ggtagcctgg gaaggctgga ggcgcttgcc
     1921 atacaactgg caggaatgcc ggggttgaat ggcgttccgc atgtgggcaa aaaagcggta
     1981 ctggtcatgt gtgccgatca cggcgtctgg gaggaagggg gcgctatttc cccaaaagaa
     2041 gtgacagcca ttcaggctga aaatatgacc cgcggaacaa ccggcgtgtg tgtgctggca
     2101 gcacaagcgg gcgctaacgt ctacgtaatt gatgttggta ttgatactgc tgagcctatc
     2161 cccgggctta tcaacatgcg tgttgctcga ggtagtggca atattgcttc agctccggca
     2221 atgagtcgcc atcaggcaga aaagttgctt ttggacgtca tatgttatac gcgggagctg
     2281 gcaaaaaacg gtgttacgct gtttggtgta ggtgaactgg ggatggcaaa cacgacaccg
     2341 gcagcggcaa tagtcagcac aatcactggc cgggctcctg aagaagtggt tgggattggc
     2401 gcaaacctgc cgacagataa actggctaat aaaattgatg ttgtgcgccg ggcgattacg
     2461 ttgaatcaac caaatccgca ggatggcgtt gatgtcctgg caaaagtggg tggatttgat
     2521 ttggtcggaa tggctggcgt gatgttaggc gctgcgtcct gcggtttacc cgtgttgctg
     2581 gatggatttc tttcttatgc tgctgcgctc gcagcctgtc agatgtctcc tgcaatcaaa
     2641 ccgtatctca ttccttctca cctgtcggca gaaaaaggag cgcgtatagc gctgtcgcat
     2701 ttggggctgg agccttatct caatatggag atgcgtttag gtgaggggag tggcgcagct
     2761 ctggcgatgc ccatcatcga agctgcctgt gcgatatata acaacatggg cgaacttgct
     2821 gccagtaata ttgttctacc ggggaatacg acttctgatt tgaacagtta aaaacgaaaa
     2881 gtctgataaa agctatactt aacggatagc tttcgcgact taggaaaggg acatgatgcg
     2941 tcgtgtaaat attttttgct catttgctct gctttttgcc agccatacta gcctggcggt
     3001 aacttaccca ttacctccag agggtagccg tttagtgggg cagtcgtata ctgtaactgt
     3061 tcctgatcac aatacccagc cgctggagac ttttgccgca caaaacgggc aagggttaag
     3121 taacatgctg gaagcgaacc cgggtgctga tgttttcttg ccgaagtccg gctcgcaact
     3181 caccattccg cagcaactga ttttgcccgc cactgttcgt aaagggattg tggttaacgt
     3241 cgctgagatg cgtctttatt actacccacc agacagtaat actgtggaag tctttcctat
     3301 tggtatcggc caggctgggc gagaaacccc gcgtaactgg gtgactaccg ttgaacgtaa
     3361 acaagaagct ccaacctgga cgccaacgcc gaacactcgg cgcgaatatg cgaaacgagg
     3421 ggagagtttg cccgcatttg ttcctgcggg ccccgataat cccatggggc tgtacgcgat
     3481 ttatattggc aggttgtatg ccatccatgg taccaatgcc aattttggta ttgggctccg
     3541 ggtaagtcag ggctgtattc gtctgcgcaa tgacgatatc aaatatctgt ttgataatgt
     3601 tcctgttggg acgcgtgtgc aaattatcga ccagccagta aaatacacca ctgaaccaga
     3661 tggctcgaac tggctggaag ttcatgagcc gttgtcgcgc aatcgtgcag aatatgagtt
     3721 tgaccgaaaa gtgccattgc cggtaacccc atctttgcgg gcgtttatca gcgggcaaga
     3781 agttgatgta aatcgcgcaa atgctgcgtt gcaacgtcga tcgggaatgc ctgtgccaat
     3841 tagttctggt tcaagacaga tgttttaaga gcgttggtaa ttagaaagca aaaagcctgc
     3901 tagaaagcag gcttttttga atttggctcc tctgactgga ctcgaaccag tgacatacgg
     3961 attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgtgaac ggggcgcata
     4021 ttaatggcag tgcatggtga tgtcaaaggg tgaaatgcat ttttttgttt gtttgctgtc
     4081 aatttcaaca aagtggtgag ctttcaatct tattgggtaa tgaaccatcg tggtgcatac
     4141 cctcctttta tagggcaggg gaacgcgaca gctgattaaa ggagcaaatg acgaatctat
     4201 cataatctct tatttgacag ttggttagct tgtacatcaa cgccaaaata aaactggcaa
     4261 gcatcttgca atctggttgt aagtaatggc ggcacttggg ccgattctta aaaaccggag
     4321 gcaacatgaa cttcagacgc ctgaaatact tcgtaaaaat tgtagatatt ggtagcctga
     4381 cccaggctgc tgaagtattg catatcgcac aaccagcgct cagccagcag gttgccacac
     4441 tggaaggtga gttaaatcaa caacttttga tccgtacaaa gcggggcgtt acgccaacag
     4501 acgccggaaa aattctctat acccatgcgc gggccatttt acgtcagtgt gaacaggccc
     4561 aactggcggt gcataacgtt ggtcaggcat tatcggggca agtctcgatt ggctttgcac
     4621 caggaaccgc tgcgtcatcc atcaccatgc ccttattaca ggcggttcgc gctgaatttc
     4681 cggagatcgt tatctatccc gagcattgct gaagtgcaac gtgcgcttta tgacgccacc
     4741 aagctggtga gcggcaggcc cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg
     4801 gtaaccacag atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac
     4861 ttaagccggg ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt
     4921 ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt
     4981 aaacttcccg gccaggctaa ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt
     5041 ggcattcggg cgatcgattt gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta
     5101 cgaaccttta atgagccgcc gttccgataa taagttctga accttcttat cagaaataga
     5161 tgtgaaggag taagtaagac acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt
     5221 ccttgggcgg atacgtgttt tattatcgtc ttaatgattt ccacatatta aaagcaagta
     5281 tgctttcaaa acacaattat aaaaaatccc gccaacaata taagttttta taaaattaaa
     5341 tataagatta tggctttaga atatttttat ttctaataga cgagattttt cctgttatga
     5401 tataatatgc cgaattaaca catgttaacg atttaccagt aatgtaaata aattttcgag
     5461 gagatcattc cagtgggacg taaatgggcc aatattgttg ctaaaaaaac ggctaaagac
     5521 ggtgcaacgt ctaaaattta tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa
     5581 ggtgaacccg atccagaatt aaacacatct ttaaaattcg ttattgaacg tgcaaagcag
     5641 gcacaagttc caaagcacgt tattgataaa gcaattgata aagccaaagg cggcggagat
     5701 gaaacgttcg tgcagggacg ttatgaaggc tttggtccta atggctcaat gattatcgcc
     5761 gagacattga cttcaaatgt taaccgtacg attgctaacg ttcgcacaat tttcaataaa
     5821 aaaggcggca atatcggagc ggcaggttct gtcagctata tgtttgacaa tactggtgtg
     5881 attgtattta aaggatcaga ccctgaccat atttttgaaa ttttacttga agctgaagtt
     5941 gatgttcgtg atgtgactga agaagaaggt aacattgtta tttataccga acctactgac
     6001 cttcataaag gaatcgcggc tctaaaagca gctggaatca ctgagttctc aacaacagaa
     6061 ttagaaatga ttgctcaatc tgaagttgag ctttccccag aagatttaga aatctttgaa
     6121 gggcttgttg atgcccttga agatgacgac gatgtacaaa aagtttatca taacgtcgca
     6181 aatctctaat tatatattaa agaaatctgt ctttatggca gatttcttta atctcatata
     6241 atccttataa aaaatataat gttcaactcg tcatattgct tataaaaaat cctcgttccc
     6301 agagaaataa tatttattat aattccagtt cttctttttc tgattacaga aggcaaagtg
     6361 acaattacgc atagtttccc gataaagacg cgacagctac atcccgcata aggcattttt
     6421 ctctttatcc ttgtacggta cttcatggaa cagagttttt gaccttgcga atcgtgatgt
     6481 ctgttgggga ggggcaattt gctcactgaa gcgtgagact cgattaagcg cacgaaacac
     6541 agaaatcaaa aaacccgggc acttttttac aaggtaaccg ggtaaaaata atttttattt
     6601 tttaactgtt ttgagactca tagagatgtc tcaaaactaa aatttggctc ctctgactgg
     6661 acttgaacca gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga
     6721 atcgtgagaa cgaggcgaat attagcgatg cccacccaca atgtcaaagc ctgttattta
     6781 aatttgaaat cgtttgctga aataatctgc attttgtcgt ttattccgac acaactggct
     6841 ttttttcaca cttttgcggt tcggatcgag ggtatttcca cagccaacgt ccagtaacca
     6901 ttcgccagta aaacagcacg gcccgcacag cccagtcggc aaacattccc atccagacac
     6961 caaccacacc ccagccaagc atgatgccca gcacataacc gacgacaacc cggcaacccc
     7021 acatgctcaa catagaaacc cacatggcgt aacgggcatc gcgagcacct ttaaatccag
     7081 cgggtagccc ccatgaggcg gaccaaatag gcataaataa agcatttagc caaatcagaa
     7141 tcacaacgac atgtttaacc tgtggatcct gggtgtaaaa cgatgccata accccggcaa
     7201 agggagccgt tagccaggcg atggccgtta atccaagagt ggaaagccag aacacatgcc
     7261 gcaactgaat ctctgcttgc gctatctgcc ctacccccaa ccttcggcct gtaatgatcg
     7321 tagaagcaga gccgagcgca cttccgggta agttgataag agccgcaatt gaaaacgcga
     7381 taaaatttcc ggcaataaca ctggtcccca tcccggcaac gaacatttgg gttaataacc
     7441 gaccactggt aaataacact gattcgacac tcgcgggaat accaatcccc atgacttccc
     7501 agataatgct aaaattcagc ggtttaaaat agctctttaa cgaaatcctt agcgcaggat
     7561 taaaaccaat cgccagcacc cacaaaattg caactgcgcc aatataacga gaaatggtta
     7621 aacccagccc tgccccgaca aatcccagtc ccggccagga gaaaaggccg taaatcaata
     7681 tgccgctaat aataatatta agaatattca ggctaccgtt aatcaatagc ggtattttcg
     7741 tattccctgc accacgaagt gccccgctac caataagagt gatggcagct gctggataac
     7801 tgagtaccgt cagctccaga taagtcaacg ccagtacttt aacttctgtc gtggcatcac
     7861 ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat aagcgttgcc aacagtacgg
     7921 caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt cgtcgatccc
     7981 gcttaccgag actaaatgcc acaacgacag tagtaccaag atcgatagca gcaaaaaaag
     8041 ccataatgac catattgaag ctgtccgcca atcccacgcc ggccatcgca tcttttccca
     8101 gccagctgac cagaaaagtg ctcagaaccc ccatcaacag gacacaggca ttctccatga
     8161 agataggaac agcaagcggg gttatctcgc gccagaacaa cactttgtag ctcttgcgtt
     8221 tagcgtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata ttcaaagccg
     8281 accttaattg cagaaagtga aaccacattt caaataatga gggagaatca gcaaagctgc
     8341 aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga aacggcaaac
     8401 agttggggaa tttaaaaatc gggtttgaca aaagattttt cgccgttaag atgtgcctca
     8461 acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg
     8521 gttcgagtcc agtcagagga gccaaattcc gattcttacg ctatcttatt agtccttatt
     8581 tgatattgat tcaataagtt agcgtgaaaa tctttccaga tgtgtattca gttttccctc
     8641 cgcatccggt taatttggtg gtcagatttg ggggcaggtt ggttcgattc tggagtgacc
     8701 cccaaatgtc tttaaacgat tcaaaaatcc gtaacttaaa accctcttca aaacccgtaa
     8761 aactgtctga ttcgcacggt ctgtatctgc tggtcaatca gggcggttcg cgtatctggt
     8821 atctcaagta tcgcttcagt ggtaaagagt cccgcgtcag tctgggcgca tatcctctgg
     8881 tttcacttgc tgaagcaaga caacgacgtg acgatatccg taagctactg acgcagaata
     8941 ttaaccccgc ccaccagcgc atgtctgata aagccgcagc gtcaccggaa aagtatttta
     9001 aagccgtggc gcttgcatgg cacaaaacca acaaaaaatg gtcgcctgat tatgccgcgc
     9061 gcatcctcgc cagcatggaa aatcatatct ttccggcgat tggtcatctg cctgtcacta
     9121 cgctgaaaac gcagaatttc actgcgttac tgcgggttat tgaggataaa ggttttctgg
     9181 aagtcgcgtc ccgaacccgg cagcaactct gcaacatcat gcgttacgcc gtacagcagg
     9241 gattcaccga aaacaacctg gcgcagcatc tggaaggcgt caccgcgccg ccggttaaga
     9301 atcactatcc cgccctgccg ctggagcggt tgcccgaatt gtttgagcgc attggcgact
     9361 atcagcaggg acggcagctt acgaggctgg cggtggtgtt gactctccac ctgtttatcc
     9421 gttccagcga gctgcgcttt gcccgctggg gtgagataga tttcagaaac aaaatctgga
     9481 ccatccccgc cacaagggaa gcgattgaga aagtccgttt ctctggccgt ggcgcaaaaa
     9541 tgcgtactcc atatatagtg ccgctttctc gtcaggctat cgccattctg aagcagataa
     9601 aggaaatctc cggccatctg gaactggtgt tccccggcga ccataacccg tataagccga
     9661 tgagcgaaaa cactgtcaac cgggcgttgc gtcttatggg ctatgatacc aaaactgatg
     9721 tctgtgggca tggattccgg acaatggcgt gtagcgcatt agtggaaact gaactctggt
     9781 cacgggatac cgtggagcgg cagatgagtc atcaggagcg caacagcgtt cgggcagcgt
     9841 atatccaccg ggctgaacat ctcgacgccc gtacagccat gatgcagtgg tggtcggatt
     9901 atctggatgt aagtcgcgag gggtacgttg cgccgtatat ttatgcgcgg cggcataagg
     9961 cggcctgaga cagtggctat ataatttgtt aaaaatgaaa aagcagatcc tgattgaatc
    10021 cggatctgct ttttacatta ggcaatcgct gccagacaat ctgcaccaga aacgccataa
    10081 ctgcaccagt tcagtaccac cgcttacctg cgccagtctg ccgtcgcctg tctgtgtcag
    10141 gactgcgcca gatcttatcg aataaacatc aaactgcgcc agtgttacgg ccctttcagc
    10201 cctttggcct tttccggtct ggcgctgtta cccttagccg ccccttaccc tttccggcct
    10261 ttttcctttg ctggcgcaat cgccagaaaa atcgtcaagc tgcaccagaa gcctgccaat
    10321 aggtcatacc gttcagaagc atcgttattg agaggaaacc gtacaaaaaa tttctacgta
    10381 gaaatttttt cggtggataa cgatgaataa agagtatttt ataatgtgat ggctatcaaa
    10441 aatcgaccat ccaaaaataa ttcattgttc attgatttat aatgaataac catgaattta
    10501 atagtcgatg attattgttt ttatcagtat tgataggaaa gtatacaaaa aattttggcc
    10561 ccaaaatttt ttgtacgaaa accgatgaat aacgcgaaat taataggaaa ccatccaaaa
    10621 ttatttgggg caaataattt tgcccgaaaa ccgatggata actcgatctt ttcaaagcaa
    10681 cggctatcaa tcagtatcct ttcttccgta aacctgcgcc acataccgcc cgcgcccgtc
    10741 gccgtgtccc aaatccatcg ccgtcattgc cagcgcttct ttctcactga atccctgcgc
    10801 cagataatga cgaatggcat cctgcgccca cgcatagcgc aacgagtgcg gggaatatgt
    10861 gccagttaac ccgagacgtg acgcctggct gtgccagtat ttcatcgcgc ttttcaggtc
    10921 gggtttatct atcagcctgc cgtggcgatc ttctgccaca accaacgcat tatccagcgc
    10981 ttttctgata gcgacagcat ccagaataac ggtttctcgc ggacgcccgc ctttggtgcc
    11041 gaacaccacg gttaaacggg gctcaccccg ctccagcgat tgcttccacg ttcttaatga
    11101 ctgcgcactt tgcacca
//
LOCUS       DAJVTY010000135        11113 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVTY010000135 DAJVTY010000000
VERSION     DAJVTY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.135"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..517)
                     /gene="hxpA"
                     /locus_tag="OQQ66_003168"
     CDS             complement(<1..517)
                     /gene="hxpA"
                     /locus_tag="OQQ66_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1905994.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGL"
     gene            complement(528..1022)
                     /locus_tag="OQQ66_003169"
     CDS             complement(528..1022)
                     /locus_tag="OQQ66_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1905995.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(1106..1561)
                     /locus_tag="OQQ66_003170"
     CDS             complement(1106..1561)
                     /locus_tag="OQQ66_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1905996.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            1899..3101
                     /gene="ackA"
                     /locus_tag="OQQ66_003171"
     CDS             1899..3101
                     /gene="ackA"
                     /locus_tag="OQQ66_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1905997.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            3176..5320
                     /gene="pta"
                     /locus_tag="OQQ66_003172"
     CDS             3176..5320
                     /gene="pta"
                     /locus_tag="OQQ66_003172"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1905998.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            5510..7030
                     /gene="yfcC"
                     /locus_tag="OQQ66_003173"
     CDS             5510..7030
                     /gene="yfcC"
                     /locus_tag="OQQ66_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1905999.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQITVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(7063..7605)
                     /gene="yfcD"
                     /locus_tag="OQQ66_003174"
     CDS             complement(7063..7605)
                     /gene="yfcD"
                     /locus_tag="OQQ66_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1906000.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(7663..8214)
                     /gene="yfcE"
                     /locus_tag="OQQ66_003175"
     CDS             complement(7663..8214)
                     /gene="yfcE"
                     /locus_tag="OQQ66_003175"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1906001.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(8270..8902)
                     /gene="yfcF"
                     /locus_tag="OQQ66_003176"
     CDS             complement(8270..8902)
                     /gene="yfcF"
                     /locus_tag="OQQ66_003176"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1906002.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            9038..9685
                     /gene="yfcG"
                     /locus_tag="OQQ66_003177"
     CDS             9038..9685
                     /gene="yfcG"
                     /locus_tag="OQQ66_003177"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1906003.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLTMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            9742..10104
                     /gene="folX"
                     /locus_tag="OQQ66_003178"
     CDS             9742..10104
                     /gene="folX"
                     /locus_tag="OQQ66_003178"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1906004.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            10125..11018
                     /locus_tag="OQQ66_003179"
     CDS             10125..11018
                     /locus_tag="OQQ66_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1906005.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
BASE COUNT         2647 a         2950 c         2804 g         2712 t
ORIGIN      
        1 ccaggccaga aagcacgcca gcgggagcat cttccgccac cacacactcc tgcggcgcaa
       61 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
      121 gctcagcggt tacaaacacc tctggtgcgg gaagcccagc tattttatgg cgcgctcgcg
      181 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
      241 tgagtaaggc gattgccccc ggaagcgcgg taataccttc ggtttccgtg gcctcgatgt
      301 gctccagaca cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
      361 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
      421 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
      481 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
      541 atttgattaa tttcgttggc gctcaaatgg tactgacgca ggcaggcatg ccacacatta
      601 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
      661 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
      721 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
      781 tcctgcaaat tagaccagga aacatgcaac gcatgataca tctccataat gtcgatgata
      841 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
      901 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
      961 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
     1021 attgttgact cctgtatcac tctactacgg tgaaaaaaaa agaaggctga gtatgccttc
     1081 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
     1141 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
     1201 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttgaggatag
     1261 cagggggtaa tggcgttaca gaacgcttgc ccagccacca taatccctgc atgggtaaac
     1321 ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc
     1381 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
     1441 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagcggccac gtctttgagt
     1501 aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
     1561 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
     1621 ggacaacatt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
     1681 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
     1741 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
     1801 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
     1861 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
     1921 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
     1981 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
     2041 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
     2101 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
     2161 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
     2221 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctacacaac ccggctcacc
     2281 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
     2341 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgcctt
     2401 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
     2461 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
     2521 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
     2581 cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
     2641 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
     2701 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
     2761 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
     2821 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
     2881 ttgtattcac tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
     2941 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
     3001 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
     3061 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
     3121 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
     3181 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
     3241 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
     3301 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
     3361 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
     3421 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
     3481 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
     3541 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
     3601 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc
     3661 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
     3721 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa
     3781 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
     3841 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
     3901 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
     3961 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
     4021 ccgtcctgac gtgctggtcg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc
     4081 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
     4141 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag
     4201 cctgcagagc ttcaaccagg aagttccggt tgacgatcac gaacgtatcg agaaagttca
     4261 ggaatacgtt gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga
     4321 gcgcagccgt cgtctgtctc cgcctgcgtt ccgttatcag ctgactgaac ttgcgcgcaa
     4381 agcgggcaaa cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
     4441 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
     4501 ccgtgttgca gcgtctcagg gtgtagaact gggtgcaggg attgaaatcg ttgatccaga
     4561 agtggttcgc gaaagctatg ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac
     4621 cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga
     4681 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
     4741 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
     4801 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
     4861 cgctgaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat
     4921 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
     4981 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
     5041 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa
     5101 ctctccggtt gcaggtcgcg ctaccgtgtt tatcttcccg gatctgaaca ccggtaacac
     5161 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
     5221 tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
     5281 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccgcagct
     5341 ttgcgctgcg gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat
     5401 ctgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatt
     5461 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
     5521 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
     5581 tgttgctatt ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
     5641 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
     5701 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
     5761 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaaggattaa cctcaggatc
     5821 gaaatacggg acagctgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
     5881 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
     5941 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
     6001 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
     6061 gctgggctat gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt
     6121 tgccagttcg tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc
     6181 ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccacta tgattggcct
     6241 gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
     6301 tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt
     6361 tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt
     6421 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
     6481 gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc
     6541 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
     6601 agggattttg ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt taaataccat
     6661 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
     6721 gttgctcttc caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt
     6781 aaccatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc agattaccgt
     6841 gctggcattc cagtttggtg atggcttcag tcacatcatt tacccaacct cagcttcgtt
     6901 aatggcaact ctcggtgtgt gcagggtgga cttccgtaac tggctgaagg tgggcgcgac
     6961 cctacttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
     7021 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
     7081 ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc
     7141 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact
     7201 gacttcatct tcctgtaggg cgaagggacc gtgagagacg cagctgaaca acgcgcccca
     7261 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
     7321 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
     7381 aaccacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
     7441 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
     7501 cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt
     7561 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
     7621 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
     7681 ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat
     7741 gccataactc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
     7801 aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt
     7861 ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg
     7921 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg
     7981 atccacttcg ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag
     8041 ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt
     8101 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac
     8161 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
     8221 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
     8281 gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat
     8341 aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg
     8401 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca
     8461 aatgttctgc catcgcgaca ctggcttttc cctcggccgt tagtggcgct tttttcgccc
     8521 ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc
     8581 aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc
     8641 aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact
     8701 caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg
     8761 gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt
     8821 cctgcaacgc cacccaagcg gataacacat aaggggagaa aaagtgggca tctgaccaaa
     8881 gcgtgatagc gggtttactc attatgtcct cggcaataaa ggaggctggc tatcaaacta
     8941 tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata
     9001 cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc
     9061 gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt
     9121 gattaaggta gacctgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc
     9181 caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgctaag
     9241 cctctttgaa tctggtgcca ttttgttgta tctggctgag aaaacggggc tctttttgag
     9301 tcatgaaacg cgtgaacgcg ccgccacatt acagtggtta ttctggcagg taggcgaact
     9361 ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta
     9421 cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagcggct
     9481 ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc
     9541 gtgggttaat gcctggactc gccagcgaat tgacctcaca atgtatccgg cagtcaagaa
     9601 ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact
     9661 cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag
     9721 taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt
     9781 cgtttacgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt
     9841 atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat
     9901 gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc
     9961 tctttgttgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg
    10021 acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc
    10081 atgaccttaa gctggcagcg ttaaccgcca tatcgggagg ctgcatgaac atagtgatca
    10141 ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc
    10201 aaatcacggt agtgacgcgt aacccacaga aagccagttc cgttctcggc cctcgggtga
    10261 cgctatggca agggctagcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc
    10321 tggccggaga accgattgct gataaacgct ggactcacga gcaaaaagag cgtctctgcc
    10381 aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc
    10441 cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg
    10501 ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag
    10561 agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg
    10621 tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg
    10681 gcgggccgat tgactctggt cggcagtatc tggcctggat tcatattgat gatatggtca
    10741 acggtattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct
    10801 acccggtacg caatgaacaa tttgcccatg cgctcggtca cgcgctgcat cgcccggcca
    10861 ttttgcgcgt ccccgcgacc gccattcgac tgttaatggg cgaatcttca gtactggtat
    10921 taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt
    10981 acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg
    11041 ccagttaact cccggtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa
    11101 gtcgggtcac cat
//
LOCUS       DAJVTY010000136        11024 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVTY010000136 DAJVTY010000000
VERSION     DAJVTY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.136"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..911
                     /gene="gap"
                     /locus_tag="OQQ66_003180"
     CDS             <1..911
                     /gene="gap"
                     /locus_tag="OQQ66_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1906006.1"
                     /translation="INDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKSIAVY
                     AEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYNV
                     NDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVDGPRG
                     KDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVSILGK
                     KATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLV
                     KTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(953..2393)
                     /gene="aldA"
                     /locus_tag="OQQ66_003181"
                     /pseudo
     CDS             complement(953..2393)
                     /gene="aldA"
                     /locus_tag="OQQ66_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2548..3390)
                     /locus_tag="OQQ66_003182"
     CDS             complement(2548..3390)
                     /locus_tag="OQQ66_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1906007.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRSTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3662..7564)
                     /gene="hrpA"
                     /locus_tag="OQQ66_003183"
     CDS             complement(3662..7564)
                     /gene="hrpA"
                     /locus_tag="OQQ66_003183"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1906008.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSGTIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVLGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7765..8370
                     /gene="azoR"
                     /locus_tag="OQQ66_003184"
     CDS             7765..8370
                     /gene="azoR"
                     /locus_tag="OQQ66_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1906009.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8421..9737)
                     /locus_tag="OQQ66_003185"
     CDS             complement(8421..9737)
                     /locus_tag="OQQ66_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCS1906010.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLISISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9727..>11024)
                     /locus_tag="OQQ66_003186"
     CDS             complement(9727..>11024)
                     /locus_tag="OQQ66_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCS1906011.1"
                     /translation="LALWHRLRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAV
                     NILLDLYKTSERIIRDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLP
                     GFYHDTLGEENRALAFEKMQSFISRLYANKSQKFDYQHEDCTGPSADQWRLLSGGPVL
                     LSPVDLAYRFMRKAMKLFGTHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLID
                     KIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEP
                     AVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSG
                     LYELFPENEQVKNSLAGLANAIEPVGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMR
                     VRSQGEMDSLVRDAGFDKCTQRIDEWGIFTVSMAVRRDN"
BASE COUNT         2741 a         2979 c         2624 g         2680 t
ORIGIN      
        1 ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat gattcaaact
       61 acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt gatgggaaaa
      121 gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg aaaggtgcag
      181 aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag gcgcatcttg
      241 atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa actatcgttt
      301 ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg gcgtcatgca
      361 ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg atagaagtcg
      421 gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat ggcccacgtg
      481 gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac actacggggg
      541 cggcaaaagc cattggtctg gagatcccgg aactgagcgg caaactgaaa ggtcatgcgc
      601 aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc ggaaaaaaag
      661 cgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac gagtcatttg
      721 gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc ggttcggtgt
      781 ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg aaaacggtcg
      841 cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc gaaaaattcg
      901 ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact cattacgact
      961 gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca tctgcgccgc
     1021 caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaag ttttcacggt
     1081 tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg ttcagatttt
     1141 gggtatagat ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcctct
     1201 tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc
     1261 gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct
     1321 accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc
     1381 tcaacccttt ccaacgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca
     1441 gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca
     1501 taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc actattaatg
     1561 acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata
     1621 gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc
     1681 atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta
     1741 cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc
     1801 ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca
     1861 ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg
     1921 aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac
     1981 aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc
     2041 cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc
     2101 tggatcttgc ctccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc
     2161 ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct
     2221 ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt
     2281 atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc
     2341 caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac
     2401 tcctgtgatt tatatgtttg ttcttggtaa atacattgtt aattaacatc aatcatcata
     2461 gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga
     2521 ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac
     2581 gggcaatttt tgacgtaacg agcgactttc catcgcctcg atgagcaccg catcgtgttt
     2641 aagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg
     2701 cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag
     2761 tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc
     2821 tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg
     2881 attgtcccca gtcatacggc ggaacgtcgc catcgtgctc cgctgcatgg tggggtcctg
     2941 aacaacgata gccgtatgaa ctcgttctac ggcctgatta aatagcgcga tgctaaagcg
     3001 tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg
     3061 ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat
     3121 agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat
     3181 accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat
     3241 agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc
     3301 ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg
     3361 cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac
     3421 tgcgtgagat aatagtgaga agctgagcac tctgaaagga ggaagtcgta tgtccttaac
     3481 cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac attattcatc
     3541 ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc gttttagaat
     3601 taataaaaaa cccggcgcta aatgcttacc gggttttaga tttattaggc aaatagcagg
     3661 gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg
     3721 taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa cggatctctt
     3781 tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct
     3841 gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca accgccagtt
     3901 tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc
     3961 cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta atgtcagaaa
     4021 gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata ttgaacactg
     4081 ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc agttcggcac
     4141 gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt
     4201 cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat
     4261 acgggttgaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt aaatacttga
     4321 tcggcgaggg aatatttagc agcagtagac ggcgaagacc attccacatt gcctgttttt
     4381 gctccagcgg gttatcaaac agtttgatcg ccacgctgtc gcgttcatcc accagcgccg
     4441 gccacgcctt cactttatag ttgccacgct tctgttcgta actttccggc agctgaccaa
     4501 aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgcg gacagcgttt
     4561 cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct tcttttagct
     4621 tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc acctgatccc
     4681 agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc
     4741 tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg
     4801 gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga gcaatcacca
     4861 attcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc tggttaagta
     4921 acggcagcgg aatatgtacg gtcacaccgt ccgcgtctgc gcctggctca aactgatagc
     4981 tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt
     5041 tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc
     5101 ggctgacttt tttccaccag ctatcgaagt gacgagcgga gattacatcg tggctgatgc
     5161 gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt
     5221 tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac
     5281 gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacga cataacgccg
     5341 gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca taaacagtga
     5401 ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg ctgtaggtac
     5461 gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc
     5521 gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt
     5581 tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg gtatattctt
     5641 gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac gcaatgtgaa
     5701 tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc accacctgac
     5761 gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcggtac
     5821 gacacaggcg acggaaggcg tttgaagaaa gcgccttttg ctgctcgcca agataattcc
     5881 acagatttac aaacgcgagg aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt
     5941 ccgatgcctg ctgtttgtcc atcggccgct cacgcgggtc ctgaatggag agcgcggacg
     6001 tgataatcat cgcctcacgc acgcagccat gtttctgcgc ttccagcacc atacgcgcca
     6061 gacgaggatc gaccggcagt tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc
     6121 tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga
     6181 tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg
     6241 cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact
     6301 ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac
     6361 gaccacaacg gcctttacgc tgattggcag acgcctgtga aatgggttca atcggcaaac
     6421 gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg tcgataacgt
     6481 atttaatccc cagtacagtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgtc
     6541 gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc gcataaagcg
     6601 gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta tcgcgaattt
     6661 cccgctcgcc gctcataaag atcagaatgt cgccagggct ttcctggctc agttcgtcta
     6721 cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc atcggcttct tcaacaatcg
     6781 ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc ggcgcattat
     6841 taaagtggcg agaaaagcgt tccgggtcga tagtcccgga agtgataatg atttttaggt
     6901 caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc
     6961 gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga
     7021 tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca cttacgtgat
     7081 cgctgaaacg cactttgtaa ccgatgcaac cgcctggctc cgttttcagc tcttccgcaa
     7141 tacggttcgc caccgttctt gccgccagac gacgcggctg ggtatggccg atcagtcctt
     7201 taatcccgcg ccccagctcc atacagattt tcggtagctg ggtcgtttta ccggaaccgg
     7261 tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaata tcctgttttt
     7321 tctgactaac tggcaaattg tccggataag tgatttccgg tcgtgccgct tcacgcagca
     7381 ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg
     7441 catcaggatt tttaaccttc ttaacaccat gcagacggcg agaaaaacgc agtctgtcgc
     7501 gcagcatcaa cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt tgttgttctg
     7561 tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga
     7621 gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc ttcgatttat
     7681 tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccaa
     7741 ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg
     7801 gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag
     7861 cactccgctg atgaaatcac cgttcgcgac ctagctgcaa atccgattcc ggtactggat
     7921 ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa
     7981 gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt
     8041 gcggcaccga tgtataactt caacatctca actcagttga aaaactattt tgacctggtt
     8101 gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct ggtaacgggt
     8161 aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc aacggaccta
     8221 gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc
     8281 ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa
     8341 gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg gactgtagtt
     8401 cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc tttgtgatct
     8461 tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc atcaacggtt
     8521 ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg cgataatccc
     8581 aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg
     8641 gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac acagaaatag
     8701 agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag aaccgcattc
     8761 tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat tttgctcacc
     8821 ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg ccagggcaat
     8881 gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt tgttatcgcg
     8941 ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca acataaccag
     9001 actggccacc ataactgaat catcaccatt agctccatca acacaatgca cgagcccgcg
     9061 cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa
     9121 cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca
     9181 tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag agatgagtaa aaaccatccg
     9241 ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca aagtagccag
     9301 cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc
     9361 gaaaatggcc atcccgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga
     9421 tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg
     9481 cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag atccagactc
     9541 cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga
     9601 ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag agatccatag
     9661 gtgaagaaaa aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc
     9721 cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat
     9781 ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct ccccttgcga
     9841 acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcat
     9901 ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgaccg gttcgatggc
     9961 attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatacag
    10021 tccagagaca attgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa
    10081 cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg
    10141 cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta cggcaggctc
    10201 gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggac
    10261 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt gtaaatgggt
    10321 tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt cgattaagcg
    10381 cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg aactgcctga
    10441 atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga acaatttcat
    10501 cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatagcacgg gtccaccaga
    10561 aagtagccgc cattgatccg ctgatggtcc tgtgcagtct tcatgctgat aatcaaattt
    10621 ttgcgatttg ttagcatata aacgactaat aaagctttgc attttttcaa atgccagcgc
    10681 cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag
    10741 agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc
    10801 acctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa taatacgttc
    10861 agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc
    10921 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga
    10981 attaataaaa aacagaccac gcagacgatg ccataacgcc agcg
//
LOCUS       DAJVTY010000137        11020 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVTY010000137 DAJVTY010000000
VERSION     DAJVTY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.137"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..111)
                     /locus_tag="OQQ66_003187"
                     /pseudo
     CDS             complement(1..111)
                     /locus_tag="OQQ66_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            388..1263
                     /locus_tag="OQQ66_003188"
     CDS             388..1263
                     /locus_tag="OQQ66_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS1906012.1"
                     /translation="MRKFILISMITLASVSLGACRQNATIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1271..2047
                     /locus_tag="OQQ66_003189"
     CDS             1271..2047
                     /locus_tag="OQQ66_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS1906013.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2216..4477
                     /locus_tag="OQQ66_003190"
     CDS             2216..4477
                     /locus_tag="OQQ66_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1906014.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4546..5721
                     /gene="senB"
                     /locus_tag="OQQ66_003191"
     CDS             4546..5721
                     /gene="senB"
                     /locus_tag="OQQ66_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS1906015.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6180..6530
                     /locus_tag="OQQ66_003192"
     CDS             6180..6530
                     /locus_tag="OQQ66_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1906016.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6673..6894
                     /locus_tag="OQQ66_003193"
                     /pseudo
     CDS             6673..6894
                     /locus_tag="OQQ66_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6915..6977)
                     /locus_tag="OQQ66_003194"
                     /pseudo
     CDS             complement(6915..6977)
                     /locus_tag="OQQ66_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7428..8840
                     /gene="ospC1"
                     /locus_tag="OQQ66_003195"
     CDS             7428..8840
                     /gene="ospC1"
                     /locus_tag="OQQ66_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1906017.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8944..9092)
                     /locus_tag="OQQ66_003196"
                     /pseudo
     CDS             complement(8944..9092)
                     /locus_tag="OQQ66_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9168..10865
                     /gene="ospD3"
                     /locus_tag="OQQ66_003197"
     CDS             9168..10865
                     /gene="ospD3"
                     /locus_tag="OQQ66_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1906018.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         3407 a         2340 c         2335 g         2938 t
ORIGIN      
        1 ccctccgttg taatgtgcca gccgggaggc cgctatcggc ggcttcttca gataacgacc
       61 caggtagcgc gccgtattcc tcccgccggc cattttcttc gacatgtaca tcaggcgcgg
      121 aactcatcgg ggcgcgggaa tgaaatccca ttcagactcc ggatagattc aagcaagccc
      181 acttatcgtc aaaatcggtg ttgcaaaaac gggagtgacc atagatccgt tttccaagcg
      241 gaccccataa tatcggctgg taaacatcac tcgctagtgc attctttttg ttggtaatga
      301 taattattta catttattct ttgatgtttt atcataatta atgttttatc aataaatata
      361 tggaaatata ttgtttcaga tgggaatatg agaaagttca tccttatttc tatgataacg
      421 ttagctagtg ttagtctggg agcctgcaga cagaatgcca ccataaagca ggatgcgcca
      481 gggcagaaag ctttcctgat tgacgggcag atttacgatt tgcacagtgg caaaattatt
      541 tctagcagcg agcttttggc ggatcttgct accgctcgac atcttattat tggtgaaaaa
      601 cacgataatg ccgagcatca tcagattgag ctttggctta ttcaaaatct gctcatccag
      661 cgaccacagg ggagtgtgtt gcttgagatg ttgacgtcag accaacagcc ccgagttaac
      721 caagttaaat gttggctgaa agataatcct gtggttcgag atagccgtgt tcaggaacta
      781 cttaactggc aaaaagggtg gtcctgggag atgtatggcg acatcgtcat gcagctatta
      841 cgtgggcctt acccgctgct caatgccaac attggccggg agcagatgtt ggcactgtac
      901 aaaaaaaacg aatttcctaa aggaaaaaaa tccactgcac ctgtggtaca agaagctctg
      961 cgtgaaacca tcatagctat gcatgaaggc aaccttgaaa gccagcaact gacgtcaatg
     1021 ctctctgttc agcagcagcg ggatcgctac atggcccgcc agcttctgag tgccccggta
     1081 ccgtcgttgt tgattgcagg tggttatcac gcgtcaaaaa acatgggcgt accgttacac
     1141 atggaagatc ttgccaccgg cacccatcca gtggtgttga tgcttgcaga aaaaggaatg
     1201 aacatcacag tagatcatgc tgactacgtg tggtttgtag cgccagatac aacaaaaagg
     1261 taaaccatgg atgatgaata ttctccactt cccacagtcg gtaaaatgga gcagctggtt
     1321 tatctgttct ctactgcttc atggactgat tttccttacg tttatctggc gtttttccga
     1381 agttcagccc gctatgtctc ctgcaccggc gatcatgctg caatgggcag aaattataga
     1441 ggctccttct tctccacttt cgcttccggt gggaatcgct caacaggagt ccgcagtgac
     1501 agaagaaaag caacaaactg aggacagaca acaacgtcct gtcactgaag acagtgatgc
     1561 aaccattgaa ataactagga agaaaaagtc atccgatgga gaaaagaaaa agacacgtcc
     1621 acctcgtaag ataaaggctc aaaccagcga cagcaacccc acagccgttt ccagtaatgc
     1681 tgcacctcag gctctggtcg aatcgtcccg tattgctgcg ccttttaaca gtgactcaac
     1741 aaagcgagat aattctgagg catcttggga aagcagagta aaaggccacc ttaaccgtta
     1801 taaacgttac cctggcgatg ccagaaaacg tgcacgaacc ggcacagccg ttgtgacttt
     1861 taccgtaaac acggaaggca ctattgtcag tagtttcttg gaaatctcgt caggaaccct
     1921 ttctcttgac cgcgaagcta ttgctgtttt agaacgcgct caaccacttc ccaaaccacc
     1981 gcctgaaata ttggagggag gattatttaa agtaaaaatg ccgattacct ttaagttgaa
     2041 ggagtaaagg gataattggc cgttcaagtt catttaactg aaatttaatt ttcattttta
     2101 tgctgaacat tttatcaaac ttttccactg tcgaaaaaat caaaaagaca gtagatcata
     2161 attaataaac aaatagttat acttatcttt attaatatta tatccaagga aatttatgaa
     2221 cgttataaaa ctggctatcg gctcaggcat attattgctc agctgcggtg cttactcaca
     2281 atccatcagt gaaaaaacta attccgacaa aaaaggagcg gcagaattca gtccgctcag
     2341 cgtttctgtc gggaagacga ccagtgagca ggaagctctc gagaaaacag gcgcgaccag
     2401 ttcccggaca acggacaaaa acctgcaatc acttgacgca acagtgcgta gtatgcctgg
     2461 tacttatact caaatagatc ctggtcaggg agcaatcagt gtgaatattc gaggcatgag
     2521 cggatttggt cgtgtaaaca ctatggtcga tggtattacc cagagttttt acggaacctc
     2581 tacctccgga acaacggcgc atggttcaac taacaatatg gctggcgtac ttatagatcc
     2641 taacttactg gtagcagttg atgttacacg cggtgacagc agtggctctg aagggatcaa
     2701 cgcccttgcc ggtagtgcaa atatgcgtac tattggcgtt gacgatgtaa tatttaacgg
     2761 taatacatat ggccttcgtt cacgtttctc tgtcggtagt aatgggctgg gacgcagcgg
     2821 aatgatcgcc cttggtggaa aaagcgacgc ttttacggat acgggaagca ttggcgttat
     2881 ggctgctgtg agtggcagtt ctgtgtactc taatttctca aatggttctg gaattaacag
     2941 caaagagttt ggttatgata aatatatgaa gcagaacccc aaatcccaac tgtataaaat
     3001 ggatatcaga ccagacgaat ttaacagctt cgaactttcc gctcgaacct atgaaaataa
     3061 atttacacgt cgtgatataa ccagtgacga ctattacatt aaatatcatt acaccccttt
     3121 ttctgaatta attgacttta acgtaacggc cagtaccagt cgcggtaatc aaaagtatcg
     3181 tgatggctcg ctgtatactt tctacaaaac ctcagcgcaa aatcgttctg acgcgctgga
     3241 tatcaacaat accagccggt tcactgtcgc ggacaatgaa ctggagttta tgctgggcag
     3301 caaactgatg cgtacccgct atgaccggac cattcactca gcggcgggcg acccgaaagc
     3361 gaatcaggaa tcgatcgaga acaatccgtt cgcaccctcc ggccagcagg atatttcagc
     3421 gctgtatacc gggctgaagg ttacgcgcgg catctgggag gcagatttca atctcaacta
     3481 cacacgtaac aggatcacag ggtacaagcc cgcctgcgat tcacgcgtta tctgcgtgcc
     3541 acagggtagc tacgatattg acgataaaga gggtggcttc aacccttcag ttcagctttc
     3601 tgctcaggta acaccatggc ttcagccgtt cattggctac agcaaatcca tgcgcgcccc
     3661 gaacatccag gagatgttct tctctaattc aggaggcgca tccatgaacc cattcctgaa
     3721 gcctgaacgt gcagaaacct ggcaggcggg ttttaacatt gataccagag atttactggt
     3781 cgaacaggat gccctgcgct ttaaggctct ggcgtaccgc agcaggatcc agaactacat
     3841 ctacagcgag tcttatctgg tttgttctgg aggtcgtaaa tgcagtatgg ctgaggtgat
     3901 tggcaatgac tgggagggca ttagcgatga atacagcgac aatatgtaca tctacgttaa
     3961 ctcggcaagc gacgttattg caaagggctt cgaactggag atggattatg atgcaggttt
     4021 tgcttttggc cgactctctt tcagccagca gcaaacagac cagccaacct ccatcgccag
     4081 cacctacttt ggcgcagggg atatgaccga actgcccaga aaatacatga cgctggatac
     4141 tggtgttcgc ttcttcgata acgcgttgac cctgggcact atcataaaat acacaggcaa
     4201 ggctcgtcgc ctgtcgcctg attttgagca ggacgaacat accggcgcaa taatcaaaca
     4261 ggatttgccg cagatcccaa cgattatcga tctctatggt acttacgagt acaaccgcaa
     4321 cctgacactg aaactttcgg tacaaaacct gatgaacaga gattattcgg aggcgctgaa
     4381 taagctcaac atgatgccag gtcttggtga cgagagccac ccagccaatt ccgcgcgtgg
     4441 cagaacatgg atatttggcg gggacattcg tttctgatga caatttccgc caggttcaga
     4501 caatacgttt tctctcttat gtcaatttta ttgcaggaac gaaaaatgaa tattttcact
     4561 ttatccaaag caccgctata cttgttaatt tcactatttt tacccacgat ggccatggct
     4621 atcgatccac ctgaacgcga actttcgcga tttgccctga aaacgaatta ccttcagtcc
     4681 cctgatgaag gcgtctatga actggcgttt gataatgcca gtaaaaaggt gtttgcagca
     4741 gtcaccgatc gtgtaaatcg tgaagccaat aaaggctatc tgtattcgtt taattcagat
     4801 tcgctgaaag tcgaaaataa atacacgatg ccataccggg cattttcgct ggcgataaat
     4861 caggataaac atcagctcta tatcggacac acccagtcag cgtccctgcg tatcagtatg
     4921 tttgacaccc caaccggtaa actggtaaga accagcgaca ggttaagttt taaagcggca
     4981 aacgctgcag attcgcgttt tgagcatttg cgccatatgg tttacagcca ggattccgat
     5041 accctgtttg tgagttatag caatatgctg aaaacggccg agggcatgaa gcctctgcat
     5101 aagctgttaa tgctcgacgg gacgacgctt gccttaaaag gcgaggttaa ggatgcttac
     5161 aaaggtacag cgtatggtct gacgatggat gaaaaaacac agaaaatcta cgttggcgga
     5221 agagattaca tcaacgaaat tgatgcgaaa aatcagacgc tgctgcgtac catcccgttg
     5281 aaagatccga gaccacaaat cacaagtgtg cagaatctgg cggtggactc cgcttctgac
     5341 cgtgcctttg tggtggtatt cgaccatgac gatcgttccg gtacaaaaga tggcctctat
     5401 atttttgact tacgcgacgg taaacagctt ggctatgtgc acacaggagc cggagctaac
     5461 gcggtgaaat acaatccgaa atataacgaa ctgtatgtca ccaacttcac tagcggcacc
     5521 atcagcgtag tggatgccac caaatacagc atcacccgtg aatttaacat gccggtctac
     5581 ccaaaccaga tggtgttgtc ggacgatatg gatacccttt acattggcat caaagaaggc
     5641 tttaaccgcg attgggatcc tgatgtgttt gtggaaggag ctaaagaacg tattctgagc
     5701 attgatttga aaaagtcgtg atcgggggaa aaaacatggg ataaaaactc cgttattcca
     5761 ctaaactggc cggactagga aaagcaaaca tctctgtcct gccggtacgc ctgttacgcc
     5821 gctccatgag gctgaggaat ctccagaaaa cgagacaggc acagcattgt acgatcaact
     5881 gatttgtgcc aataattcaa tgaaatcaca gctatgcctg ttcacgtata ggaaattgaa
     5941 aaactaagcg ataccactgt ccgcgtctta tgcctcgtgc ggtaagctgt ttttcacatc
     6001 acgccggtgt ttttttcttt ctgtggacgg ttttaatact gtaatcggcc gttttttata
     6061 cttaccccga ttcccgggcg cacttatcct gcatcacccc gggatatacc tcagcagact
     6121 gatatcccgc gcgtttttct tcagcccctc cacattcagt cccgcgatgg cccggcggta
     6181 taccatccgg ccgccacaca gcacgcattc gaacgggtca cgactcagga actgtttcac
     6241 catttgtgca tagcacactt tcgccactgg ttccggttta tcctgttttt ataacggtaa
     6301 ttgcagacca tgacaaaccg cagccgtcag gctgcctact cgagtcacaa ggctctctgt
     6361 gtccgatatg ccggcagcgc attacccatg aaactggctg gaacgtacac cataaggtga
     6421 agaaagttat ggggggcggt gaagaattat ctaaccttgt cttgttacat ccaaattgcc
     6481 atcgacagtt acacagttgt gaagccggct ctctcaaaaa gggcttataa aggcttgagc
     6541 ggtgtaccgg gaaaccggta tgcaccgttc ttaggggagg gcagtccagc aatgaactgt
     6601 ccttacccga caaacatcca aaacgaacgg aacgtgctgg aatccgcata taacccgttg
     6661 taaagtgagg aaatggacac ctcacttgct catgagaacg cccgcctgcg ggcactgttg
     6721 cagacgcaac aggacactat ccaccagatg gctgaataca accgcctgct ctcacagcgg
     6781 atggcggctt atgcttccga aatcaaccga ctgaaggcgc tggttgcgaa actgcagcgt
     6841 atgcagttcg gtaaaagccc agaaaaactt cgcgcaaaaa ccgaacggca gattccattt
     6901 tcacgggcga tctacgccac acaggctctg ggttgcagtg actgctccat taaggcgatt
     6961 ttgaactcat aagggaagta acaaaaaaac tgcaaaagta caataattat atatggccaa
     7021 actaacctct attaaaaaaa catcagtttt tcacacttag ttgagcttca tgaaaaaatc
     7081 atcgaggatt tcattgcaga atacacattc cactaactgg aagcactcca taagaggaat
     7141 cagagaaagt atcgtttttt tatagtaaaa ttcattgctg ttcaataaaa ttgatataat
     7201 tattaaatta tggaaataat ttcacaaaaa agattaactt gagttcttaa gcatcggatt
     7261 ttagatattt tgaaatatct ggcatcacat aacatcaaaa gtatataata cccttatcta
     7321 attacatgca gtatattgta tatatactca aaaattgtaa ttcataccct actatatctt
     7381 gtggggaaag atattaatta aaactgtttt catataaggt tcattttatg aatatatcag
     7441 aaacactgaa ctcagcaaat acccaatgca atatagattc tatggataac agattacata
     7501 cattgtttcc aaaagtgaca tcagtgcgaa acgctgcaca acaaactatg ccagatgaaa
     7561 aaaatttaaa agatagtgca aatattatta aagatttctt taggaaaact atagcagcac
     7621 aaagttatag tagaatgttc tctcaaggct ctaactttaa atctttaaat atagcaattg
     7681 atgcaccatc agacgctaaa gcctcattta aggctattga gcaccttgac agattatcga
     7741 agcattatat atctgaaata agggaaaaac ttcatcctct ttctgcagag gaactcaatt
     7801 tgctttcgct aattattaat tctgatttaa tcttcagaca tcaaagtaat tctgatttgt
     7861 ctgataaaat tttaaacatt aagtcattca ataaaattca gtctgaagga atatgcacaa
     7921 aacgaaacac atacgctgat gatataaaaa aaatagctaa tcatgacttt gtgttttttg
     7981 gcgttgaaat ctctagccat cagaaaaaac accccctgaa tacaaaacat cacactgttg
     8041 attttggtgc aaatgcgtat atcattgatc atgactctcc atatggatat atgacattaa
     8101 ccgatcactt tgataatgct attccacctg ttttttacca tgagcaccaa tcatttttag
     8161 ataaattttc agaggttaat aaagaagtta gtcgatacgt acatggaagt aaaggaatta
     8221 tagatgtacc aatattcaat actaaagata tgaagttagg gctcggatta tacctgattg
     8281 actttattag aaaaagtgaa gaccaaagct tcaaggagtt ttgctatgga aaaaatcttg
     8341 cccctgtgga tctggataga atcataaact ttgtttttca gccagagtac catataccta
     8401 ggatggtaag tacagaaaac ttcaaaaaag ttaagattag agaaatatcc ttagaggagg
     8461 ctgttacagc atctaattac gaagaaatta acaagcaggt cactaacaaa aaaattgctc
     8521 tccagactct ttttctttcg attactaatc aaaaagagga tgtcgcctta tatatattat
     8581 ctaattttga gataactaga caagatgtta tttccataaa gcatgagttg tatgatattg
     8641 agtatctact tagcgctcat aattcaagct gtaaagtact tgagtatttt atcaataagg
     8701 ggttggttga tgtaaacaca aagttcaaaa aaactaatag tggggattgt atgttggata
     8761 acgcaataaa atatgagaat gcagaaatga taaaactatt attgaaatat ggtgcaacat
     8821 ctgacaataa atatatttaa tcaaaattga atatcgttta gcaaaaggca gtatttgcac
     8881 aaaatatcga tatattgttt attgtcagta tggctcaatg tgataatagt tggaaagttt
     8941 gatgggtttc gccccgttgt agcggtagtc gagctggaag gtcttcaggc actgcttaca
     9001 gcgatagagc agcccccctg aactggaatg gccgttccgg ataccccctg agtttcagag
     9061 taacggggac aaaccacatc aatctttgcc atcaatcatc caaagggcaa agagtacaac
     9121 aacactaagt ctgcgtcaca acccatcaat gaaaggaata tatacatatg ccatcagtaa
     9181 atttaatccc atcaaggaaa atatgtttgc aaaatatgat aaataaagac aacgtctctg
     9241 ttgagacaat ccagtctcta ttgcactcaa aacaattgcc atatttttct gacaagagga
     9301 gttttttatt aaatctaaat tgccaagtta ccgatcactc tggaaaactt attgtctgtc
     9361 gacacttagc ttcctactgg atagcacagt ttaacaaaag tagtggtcac gtggattatc
     9421 atcactttgc ttttccggat gaaattaaaa attatgtttc agtgagtgaa gaagaaaagg
     9481 ctattaatgt gcctgctatt atttattttg ttgaaaacgg ttcatgggga gatattattt
     9541 tttatatttt caatgaaatg atttttcatt ccgaaaaaag cagagcacta gaaataagta
     9601 catcaaatca caatatggca ttaggcttga agattaaaga aactaaaaat gggggggatt
     9661 ttgtcattca gctttatgat cccaaccata cagcaactca tttacgagca gagtttaaca
     9721 aatttaactt agctaaaata aaaaaactga ctgtagataa ttttcttgat gaaaaacatc
     9781 agaaatgtta tggtcttata tccgacggta tgtctatatt tgtggacaga catactccaa
     9841 caagcatgtc ctccataatc agatggccta ataatttact tcaccccaaa gttatttatc
     9901 acgcgatgcg tatgggattg actgagctaa tccaaaaagt aacaagagtc gtacaactat
     9961 ctgacctttc agacaatacg ttagaattac ttttggcagc caaaaatgac gatggtttgt
    10021 caggattgct tttagcttta caaaatgggc attcagatac aatcttagca tacggagaac
    10081 tcctggaaac ttctggactt aaccttgata aaacggtaga actactaact gcggaaggaa
    10141 tgggaggacg aatatcgggt ttatcccaag cacttcaaaa tgggcatgca gaaactatca
    10201 aaacatacgg aaggcttctc aagaagagag caataaatat cgaatacaat aagctgaaaa
    10261 atttgctgac cgcttattat tatgatgaag tacacagaca gatacccgga ctaatgtttg
    10321 ctcttcaaaa tggacatgca gatgctatac gcgcatacgg tgagctcatt cttagccccc
    10381 ctctcctcaa ctcagaggat attgtaaatt tgctggcctc aaggagatat gacaatgttc
    10441 ccggacttct gttagcattg aataatggac aggctgatgc aatcttagct tatggtgata
    10501 tcttgaatga ggcaaaactt aacttggata aaaaagcaga gctgttagaa gcgaaagatt
    10561 ctaatggttt atctggattg tttgtagcct tgcataatgg atgtgtagaa acaattattg
    10621 cttatgggaa aatacttcac actgcagacc ttactccaca tcaggcatca aaattactgg
    10681 cggcagaagg cccaaatggg gtatctggat taattatagc ttttcaaaat aggaattttg
    10741 aggcaataaa aacttatatg ggaataataa aaaatgaaaa tattacacct gaagaaatag
    10801 cagaacactt ggacaaaaaa aatggaagtg attttctaga aattatgaag aatataaaaa
    10861 gctgaatatt atctcgtggt gatacactga atttgaggta accggctcat ttaaaccgtc
    10921 tggtctgtta cctccggttc cacaaaaata atgtccatca ttttgaagtg gacactatcg
    10981 tatggaatac cggacctgga tcactgaagc tttacgcctt
//
LOCUS       DAJVTY010000138        10957 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVTY010000138 DAJVTY010000000
VERSION     DAJVTY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.138"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            250..447
                     /locus_tag="OQQ66_003198"
     CDS             250..447
                     /locus_tag="OQQ66_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906019.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(397..1590)
                     /locus_tag="OQQ66_003199"
     CDS             complement(397..1590)
                     /locus_tag="OQQ66_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906020.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1726..3450
                     /gene="iucA"
                     /locus_tag="OQQ66_003200"
     CDS             1726..3450
                     /gene="iucA"
                     /locus_tag="OQQ66_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1906021.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3451..4398
                     /gene="iucB"
                     /locus_tag="OQQ66_003201"
     CDS             3451..4398
                     /gene="iucB"
                     /locus_tag="OQQ66_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1906022.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4398..6140
                     /gene="iucC"
                     /locus_tag="OQQ66_003202"
     CDS             4398..6140
                     /gene="iucC"
                     /locus_tag="OQQ66_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1906023.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6137..7474
                     /gene="iucD"
                     /locus_tag="OQQ66_003203"
     CDS             6137..7474
                     /gene="iucD"
                     /locus_tag="OQQ66_003203"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1906024.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7480..9675
                     /gene="iutA"
                     /locus_tag="OQQ66_003204"
     CDS             7480..9675
                     /gene="iutA"
                     /locus_tag="OQQ66_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1906025.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9980..>10957)
                     /locus_tag="OQQ66_003205"
     CDS             complement(9980..>10957)
                     /locus_tag="OQQ66_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1906026.1"
                     /translation="CEHLPLYRQSEIFARQGVELSRALLSNLVDACCQLMTPLNDALY
                     RYVMNSRKVHTDDTPVKVLAPGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQ
                     GKHPEQHLSPFRGILQADAFNGYDRLFSAEREGGALTEAGCWAHARRKVHDVYISTKS
                     ATAEEALKLIGELYAIEHEIRGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKC
                     RLRDAFRYIRKHWVALCNFSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERG
                     ALLYGLIGTCRLNGIDPEAYLRYILSVLPEWPSNRVDELLPWNVALTNK"
BASE COUNT         2604 a         2856 c         2897 g         2600 t
ORIGIN      
        1 catgtcagtg aaaaagaaac ctggcgttgg gttgaaggat ataataaagc aacagaactg
       61 gctcgccaat gccctgatac ccatgtattg tcaatttctg atcgtgaagg cgatttttat
      121 gacctgtttg aacgcgcaga gcagactccc ggaataaaag ctgactggct tgtcagaatg
      181 aaatttaata acagagcaac gctgaatatt aatggtaaac gggatcatag gttgttgcac
      241 gaaagaatca tggagattac accgcagcaa ctggttgaat tcactatacc tgatgggcgg
      301 gggcagcaag ccagaacact gcctgacatg ctttcatcag acgcaggtcc tctctcatat
      361 cctctgattt actccagtaa ggtgatgatc atatctttat ttgatagtac ggtaatgcca
      421 gataaataca gacacaatgg taaataatat agttgcaaat aatgctgcca taatcagcgc
      481 gattgcgaca cctgttttcg ataccagcgg caccagcaaa gcgcacagtc catagccaag
      541 tgtgtgactg gtagcgagcc agcccgcgcc tgcgccatca gccagcttat cattcagtag
      601 aagttgataa gcgggtgttg ccagagctgc tccaaatgac agcactgcac agcccgggta
      661 aaacaaccat agctgttcgg agagcatgat agccagacca ccactcatca acactccggc
      721 actcaggagc agggccaccg gagtcaggcg ctgcggacgg actaccccga actgcgcgat
      781 aagcgcagct actgcggaca gacccaacag ccaggccacc tgttggctaa tggcggtggt
      841 atccgtgacg aactggcgag taagggcagg cgaaagtcca agctgcatca tgctcaccgc
      901 cgcagccagt aatattgcgc aaagcagata aggcagacaa tcccgcttca gactgacgct
      961 cttgcactcc ggtgtgggct gtggtggtgt gccgggcaac cgcagcagca tcagcagcgc
     1021 cagcactggt gctgccatca gtagccccag tggcgccaga ggatgaatgg ccagcattgc
     1081 tgccgcgcac agcggcccga atagcctgcc gcaactcagg ccggagctga tggttgccag
     1141 agcggccatg cgattccctt ctccagctct ctgcaatgcc cagacctgac aggctggcac
     1201 cattgccgac acggtcagcc cgtaggcgat ccgggcgatg attaatattc ccagtcccac
     1261 cgctgttgtt atggcgctgg tagccatcag cacgcttccc agccctagca gggtaaagct
     1321 tatgccatat cctcccagag cccacaacac cacaggttta ctgccagtac gggaaatttg
     1381 ctttccccac cacggggacg atggcagaaa cagcattgag ccaagcatca gcagggcagc
     1441 ccatacagac agactcagat ttgtctgtat gaccagaaca gggagcacaa ccagcagacc
     1501 gttctgtccg ataccgagaa gcccggcact gaacgcaagt ggccagcagg acagtgtttt
     1561 ttggggcgta tcttcgatcc ctggtgacat tataatgctt caactccatg tattaattgt
     1621 gtttatttgt aaaattaatt tatctgacaa taacatttat tgttgataat gagaatcatt
     1681 atcgacataa ttgttattat tttactgtgt gggagctgtt tgactatgac cctgccctct
     1741 gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga atgcactgat tcgtgaaacc
     1801 acagactgga aactgacaga atacccgcca gacgaattgc tcatcccgct ggatgagcag
     1861 aaatcgctcc atttcagagt ggcttatttc tccccaaccc agcatcaccg ctttgcattt
     1921 cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg actttaccac tctctcccgg
     1981 cttattattg ataagctacg ccatcaactg tttctgcccg ttcccctctg cgaaactttc
     2041 caccagcgcg tgctggaaag ccatgtccat acgcaacagg caattgatgc ccgtcatgac
     2101 tgggccgctc tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc gctgctgaca
     2161 ggacacgctt tccaccctgc gcctaagtct catgaaccgt ttaaccggcg ggaggctgaa
     2221 cgctacctgc ctgatatggc accccacttc ccactgcggt ggttttcggt ggataaaacg
     2281 caaatcgccg gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg atttgccgca
     2341 gagaatgcgc ctcagttact caacgaatta agtgacaacc aatggctgtt cccgttgcac
     2401 ccgtggcagg gggaatatct tttgcagcag gggtggtgcc aggcacttgt tgctaaaggg
     2461 ctgattaaag acttaggtga ggccggcacg tcgtggctgc cgaccacctc ttcccgttcc
     2521 ctctactgtg ccaccagccg cgatatgatc aagttctccc tgagcgtacg gctgaccaac
     2581 tccatccgta ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc acgcctggct
     2641 caaaccgacg gctggcagat gctacaggtc cgcttcccga ctttccgggt aatgcaggag
     2701 gatggctggg ccgggctgct cgatcttaac ggcaacatca tgcaggaaag tctgtttgcc
     2761 ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga ctaacgtact ggtctccctg
     2821 actcaggccg cacctgatgg cggtgattcg ctgctggttt cggcggtaaa acgcctgagc
     2881 gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc atactgtcag
     2941 caagtgctaa agccgctgtt tacggctgaa gcggattacg gcctggtgct gctggcgcat
     3001 cagcaaaata ttcttgtcca gatgcttggg gatctgccgg tcggatttat ttaccgtgac
     3061 tgtcagggca gcgcttttat gcctcacgcg acagactggc tcgattctat tggcgaggcg
     3121 caggcggaaa atattttcac ccatgagcag ttgctgcgct atttccctta ttacctgctg
     3181 gttaactcca cttttgctgt gaccgctgcg ctgggggctg ccgggctgga cagcgaatcg
     3241 aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt gactcataaa
     3301 acctgtctca actacgtgct ggaaagtccg tactggaacg taaaaggtaa ctttttctgt
     3361 tatctgaacg atcataacga gaacaccatt gttgaccctt cggtgatcta tttcgatttc
     3421 gctaacccgc tgcaggctca ggaggtctga atgtctgagg caaacattat tcacagcaga
     3481 tatggactgc gctgtgaaaa actcgacaag cccctgaatc ttggctgggg actggacaat
     3541 agcgcggtgt tgcactgtcc cggggagctg ccgacagggt ggctgtgcga tgcgctggat
     3601 cagatattta tcgccgcacc acaactgtca gcagtggctc tcccctgggc cgaatggcgt
     3661 gaggagccac aggcgctgac gctttttggg caggtaaaaa gcgacattat ccatcgtact
     3721 gccttctggc agttaccgtt atggttgagt tcaccggcaa accgggcctc cggtgaaatg
     3781 gtttttgatg cagagcgtga gatttatttc ccgcagcgcc caccccgtcc acagggtgag
     3841 gtttaccgcc gttacgatcc acgtattcgc aggatgctga gtttccgcat tgccgatccc
     3901 gtttctgatg cagaacgttt cacccgctgg atgaatgatc cgcgcgttga gtatttctgg
     3961 gagcaaagtg gctcactgga ggtacagacc gcctatctgg agcgccagct caccggtaaa
     4021 catgcattcc cgctgattgg gtgcttcgac gatcggccat tcagctattt cgaaatctac
     4081 tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc agccctttga ccgtggcctg
     4141 catctgctgg ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag ctggctgcgc
     4201 gggctgacac attacctgct gctggatgag ccccgtacgc agcgcacagt actggagcca
     4261 cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg gacaattaaa
     4321 gagttcgact tcccacataa gcgctcgcgc atggtgatgg cggatcgtca tcacttcttc
     4381 acggaggtcg gtctgtaatg aatcacaagg actgggattt tgttaaccgt cggctggtgg
     4441 caaaaatgtt gtctgagatg gagtatgagc aggttttcca cgcggaatct cagggcgatg
     4501 accactactg cattaacctg ccgggagcac aatggcgctt catcgctgaa cgtggtatct
     4561 ggggctggct gtggattgat gcccaaacgt tgcgctgcac ggacgaacca gtactggcgc
     4621 agacgctgct gatgcagcta aagccggtgc tgtcaatgag tgatgccact gttgcagagc
     4681 atatgcagga tttgtatgcc acgctactgg gcgacctgca actgctgaag gcccgtcgcg
     4741 gactgagtgc cagtgacctg attaatcttg atgccgatcg tctgcaatgc ctgctgagcg
     4801 gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggcaaagag gcgctggaac
     4861 gatatgcgcc agagtatacc aacaccttca gactgcactg gctggcggta aaacgtgaac
     4921 atatgatctg gcgctgcgat aacgatctgg atattcagca gttgttgact gccgcaatgg
     4981 atccgcagga gtttacacgc ttcagtcagg tctggcagga aaacgggctg gatcacaact
     5041 ggctaccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgccaca gacttcatcg
     5101 ctgattttgt cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag tggctcgccc
     5161 agcagtcgct gcgtaccctg accaacgcca gccgccgggg agggctggat atcaagctgc
     5221 cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatat atcgctgccg
     5281 gaccactggc ttcacgctgg ctgcaacagg tttttgcgac cgacgccacc ctggtgcaaa
     5341 gcggcgcagt gatccttggt gaaccggcgg caggctatgt gtcccatgaa ggctatgccg
     5401 cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg cgggagaatc
     5461 cgtgccgctg gctgaaaccg gatgaaagtc ctgttctgat ggcaacactg atggagtgcg
     5521 acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg ctccggactg gacgctgaaa
     5581 cctggcttac gcaattgttc cgggtggttg tggttccgct gtatcacctg ctttgccgct
     5641 acggtgtcgc gcttattgca cacggacaaa atataacgct cgccatgaaa gagggggtac
     5701 cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg gctggtgaaa gaagcgtttc
     5761 ccgaaatgga ttctttgcct caggaggtgc gtgatgccac atcccgtctg agtgcggact
     5821 acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt atttcgccac
     5881 tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca gcagtgttga
     5941 gtgattacat gaacaaacat ccacaaatgg cagagcgttt tgcgcttttc tcactcttca
     6001 ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggccg gacctggatg
     6061 gcggcagtcg catgctgccg aattaccttg agaatctgca aaatccgctg tggctggtaa
     6121 ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtcggga cagggccatt
     6181 taatctcagc attgctgcat tgtcacatca gatcgaagaa ctgaactgtc tcttctttga
     6241 cgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc atatgcagac
     6301 cgtctttctg aaagatctgg tcagtgcagt tgcacctaca aatccctaca gctttgttaa
     6361 ctatctggtg aagcacaaaa agttctatcg cttccttaca agcaggctac gtacagtatc
     6421 ccgtgaagag ttttctgact atctccgctg ggctgctgaa gatatgaata acctgtattt
     6481 cagtcatacc gttgaaaata ttgatttcga caaaaaaagt cgattgtttc tggttcagac
     6541 cagtcgggga gaatattttg cccgcaatat ctgccttggt acaggaaaac aaccttattt
     6601 accaccctgt gtgaagcatg tgacacaatc ctgtttccat gccagtgaaa tgaatcttcg
     6661 tcggccggac ctgagtggaa aacggataac cgtggttggt ggaggacaga gtggtgcaga
     6721 cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa actgggtctc
     6781 acggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt attttacacc
     6841 tgaatatatt tcaggcttct ccggactgaa ggaagatatt cgccatcagt tactggatga
     6901 gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt atcgtgagtt
     6961 gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac ccagtcgctc
     7021 ggtaataact ctggaaagta gtggtccggg ctggaggtta ttgatggaac atcatctgga
     7081 tcagggcaga gagagcctgg aaagtgatgt ggtgattttc gccacaggtt accgttctgc
     7141 attgccacaa atacttccct cactgatgcc cttgatcacc atgcacgata agaacacctt
     7201 taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagaaaata acatctttgc
     7261 ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc cagctcagcc tgatggcctg
     7321 gcgatctgca cgtattctta atcgcgtact ggggcgtgat ttattcgatc tcagtatgcc
     7381 gcccgcactg attcagtggc gcagcggcag ccggaaaaaa ccgcagccgg aggctgcttc
     7441 tttaactcac tatacagcaa atattcagga ataacgatga tgcgcaaaaa gtatatgccc
     7501 cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag ctgtcgccca gcaaaacgat
     7561 gataatgaga tcatagtgtc tgccagccgc agcaatcgaa ctgtagcgga gatggcgcaa
     7621 accacttggg ttatcgaaaa tgccgaactg gagcagcaga ttcagggcgg taaagagctg
     7681 aaagacgcac tggctcagtt aatccccggc cttgatgtca gcagccagag tcgaaccaac
     7741 tacggtatga acatgcgtgg ccgcccgctg gttgtcctga ttgacggtgt gcgcctcaac
     7801 tcttcacgtt ccgacagccg acaactggac tctgtcgatc cttttaatat cgaccatatt
     7861 gaagtgatct ccggcgcgac ggccctgtac ggtggcggga gtaccggagg gttgatcaac
     7921 atcgtgacca aaaaaggtca gccggaaacc atgatggagt ttgaggctgg cacaaaaagt
     7981 ggctttaaca gcagtaaaga tcacgatgag cgcattgccg gtgctgtctc cggcggaaat
     8041 gaccatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg gtttgacggt
     8101 aacggcgatg ccaccctgct tgataacacc cagaccggcc tgcagcactc caatcggctg
     8161 gacatcatgg gaaccggtac gctgaacatc gatgaatccc ggcagcttca actgataacg
     8221 cagtactata aaagtcaggg agacgacaat tacgggctta atctcgggaa aggcttttcc
     8281 gccatcagcg ggagcagcac accatacgtc agtaaggggc tgaattctga ccgcattccc
     8341 ggcactgagc ggcatttgat cagcctgcag tactctgaca gtgatttcct gggacaggaa
     8401 ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt actacccgtt cccgacggta
     8461 aatgcgaata aacaggcgac ggctttctcc tcgtcacagc aggataccga ccagtacggc
     8521 atgaaactga ctctgaacag ccaactgatg gacggctggc aaatcacctg ggggctggat
     8581 gctgagcatg agcgctttac ctccaaccag atgttcttcg atctggctca ggcaagtgct
     8641 tccggagggc tgaacaacca taagatttac accaccgggc gctatccgtc atatgacatc
     8701 accaatctgg cggccttcct gcaatccagc tatgacatta atgatatttt taccgttagc
     8761 ggtggcgtac gctatcagta tactgagaac agggtagatg atttcatcga ctacacgcag
     8821 caacagaaga ttgctgccgg gaaggcgata tctgccgacg ccattcctgg tggttcggta
     8881 gattacgata actttctgtt caatgctggt ctgctgatgc acatcaccga acgtcagcag
     8941 gcatggttca atttttccca gggggtggca ttgccggatc cggggaaata ttatggtcgc
     9001 ggcatctatg gtgcagcagt gaacggccat ctttccctga caaagagcgt gaacgtcagc
     9061 gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac tgggctggcg ctttaccggt
     9121 gacaacctgc ggactcaaat cgcggcatat tactcgcttt ccaataagag cgtggaaagg
     9181 aataaagatc tgaccatcag tgtgaaggac gacaggcgcc gtatttacgg cgtggaaggt
     9241 gcggtggact acctgatccc ggatactgac tggagtaccg gtgtgaactt caatgtgctg
     9301 aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg acgtgaagga atcaagtcca
     9361 tcgaaagcga cagcttacat taactgggcg ccggaaccgt ggagtctgcg tgtacagagc
     9421 accacttctt tcgacgtaag cgatgcagag ggtaacgata ttaatggtta cactaccgtc
     9481 gattttatca gtagttggca gcttccggtg ggaacactca gcttcagcgt tgagaacctc
     9541 ttcgaccgtg actataccac tgtctgggga cagcgtgcac ctctgtacta cagcccgggt
     9601 tacggccctg cttcactgta cgactacaaa ggccggggcc gaacctttgg tctgaactac
     9661 tcagtgctgt tctgacgggg atgcttttgc aacaagggta tgctgtaaac aacatggtcc
     9721 ttggtggcca tgttgtttac agccagtata atatacagcc ttaacaagat attccgtctc
     9781 cggaatggac tgttcaccac tgaataacat tatttctgtt ttatggatct ttgactgaaa
     9841 tttatcgcat atcctcattt cattgtccgt ctgaaaacta accttacaag taagcgtcgt
     9901 ctcagcaccg tctggcagat cctgaaattc ctgagaggat gatctgcgcg taagcgtcaa
     9961 cggagcaccg tattgacgct tatttattgg tgagtgctac gttccatggc aggagttcgt
    10021 caacacggtt ggaaggccat tccggcagta cgctcaggat atagcgcaga tacgcttccg
    10081 gatcgatacc gttcagtcgg caggtgccga tcagcccgta cagtagcgca ccacgctctc
    10141 caccgtgatc gctgccgaag aacataaagt ttttctttcc gagacagact gcacgaagcg
    10201 ctctttccgc ggcattatta tccgcctcag ccagaccatc atcactgaag ttgcacaacg
    10261 caacccagtg cttcctgata taccggaacg catctctcag acggcatttt ttcgataacg
    10321 tgtgttcttt ctcctgcatc agcttataca gggaagtcag tagcggttta ctctgcattt
    10381 gcctgaccgc caggcgttca gacaccggca acccgcgtat ttcgtgctcg atggcgtaca
    10441 gctcaccgat tagtttcagg gcttcttccg ctgtcgcgct tttggtactg atatatacat
    10501 cgtggacttt gcgccgcgca tgagcccagc atcctgcttc cgtcaacgcg ccgccttctc
    10561 gttcggcact gaacagccga tcgtaaccat taaacgcatc tgcctgcagg ataccccgga
    10621 agggactaag gtgctgctcc ggatgtttac cctgatggtc cggtgagtag gcgaaccaga
    10681 ccgccggagg ctctggcgaa ccggcattcc tgtcatcccg gacatacgtc cagatatatc
    10741 ctgttttcgc cttcttcctg cccggtgcca gcacttttac tggtgtgtca tcagtgtgaa
    10801 ctttgcggct gttcattaca taacggtaca gagcatcatt cagcggcgtc attaactggc
    10861 agcacgcgtc aaccaagttg gagagtaatg cacggctcag ttcgacaccc tgacgggcaa
    10921 aaatttcact ctgacgatac agtggcaggt gttcgca
//
LOCUS       DAJVTY010000139        10783 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVTY010000139 DAJVTY010000000
VERSION     DAJVTY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.139"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..613
                     /locus_tag="OQQ66_003206"
                     /pseudo
     CDS             1..613
                     /locus_tag="OQQ66_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            610..1224
                     /locus_tag="OQQ66_003207"
     CDS             610..1224
                     /locus_tag="OQQ66_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS1906027.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OQQ66_003208"
     CDS             complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OQQ66_003208"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1906028.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2999..3457
                     /gene="gpt"
                     /locus_tag="OQQ66_003209"
     CDS             2999..3457
                     /gene="gpt"
                     /locus_tag="OQQ66_003209"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1906029.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3549..4793
                     /gene="frsA"
                     /locus_tag="OQQ66_003210"
     CDS             3549..4793
                     /gene="frsA"
                     /locus_tag="OQQ66_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1906030.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4851..5252
                     /gene="crl"
                     /locus_tag="OQQ66_003211"
     CDS             4851..5252
                     /gene="crl"
                     /locus_tag="OQQ66_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1906031.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="OQQ66_003212"
     CDS             complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="OQQ66_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1906032.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6634..7737
                     /gene="proB"
                     /locus_tag="OQQ66_003213"
     CDS             6634..7737
                     /gene="proB"
                     /locus_tag="OQQ66_003213"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1906033.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7749..9002
                     /gene="proA"
                     /locus_tag="OQQ66_003214"
     CDS             7749..9002
                     /gene="proA"
                     /locus_tag="OQQ66_003214"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1906034.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9117..9192
                     /locus_tag="OQQ66_003215"
     tRNA            9117..9192
                     /locus_tag="OQQ66_003215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Thr,seq:cgt)
     gene            complement(10247..10681)
                     /locus_tag="OQQ66_003216"
     CDS             complement(10247..10681)
                     /locus_tag="OQQ66_003216"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906035.1"
                     /translation="MGYDSRLDRLATTSWHSFFNNITARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            complement(10597..>10783)
                     /locus_tag="OQQ66_003217"
     CDS             complement(10597..>10783)
                     /locus_tag="OQQ66_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906036.1"
                     /translation="EPDERESYELFMEKHFGGLVDSRRAKNGDNEYMAWDMTLGWIVW
                     QQRAGIHFSTISQQEVK"
BASE COUNT         2562 a         2656 c         2784 g         2781 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tctcgcatca tggtttgcct caaggcagtg
       61 acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg tgtattaatc
      121 gccagatgat cgctttgcgc ataatgcaac aagttaaggc cacgggaagt ccggttctgg
      181 atgtggcgca taactttgtt agcgcgtgtc aaatcggcgt caacagggct ggttgcatcg
      241 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac gtggtgatta
      301 ctcctggatt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
      361 agggcgtaaa tgggggcgca ccgagtgtaa atggcgtctg gcagcgaaat acacggtgac
      421 gcaactctcg cggactgaac ttggcagtcg gataatttgt cgcgataaac aactcatctt
      481 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
      541 cggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
      601 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
      661 gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga cgtcgcggta
      721 acggtgttgg aaacagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
      781 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gtggcactat tcagtggatt
      841 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
      901 tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct gcgttcgtcg
      961 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
     1021 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
     1081 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
     1141 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
     1201 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
     1261 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
     1321 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
     1381 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
     1441 tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt
     1501 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
     1561 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
     1621 tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt attgtcagtc
     1681 atggtcacca caccgacgtt cagggaggtt tcaaccacac ctttggctac atcggagttg
     1741 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
     1801 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
     1861 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt cagtgcgtcg
     1921 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
     1981 gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag
     2041 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg
     2101 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
     2161 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
     2221 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca
     2281 aaagcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cggaccgtga
     2341 accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca
     2401 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
     2461 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
     2521 aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
     2581 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
     2641 gcgagttgct cttcatgata ggacgggtgc ggaatagaac agattttggc aaaaatatcc
     2701 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
     2761 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
     2821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     2881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     2941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3121 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3181 cagctacgat cacgacaacc agcgagagct taaagtgctg aaacgcgcag aaggcgatgg
     3241 cgaaggcttc atagttattg atgacctggt ggataccggt ggtactgctg ttgcgattcg
     3301 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3361 ggttgatgac tatgttgttg atattccgca agatacctgg atcgaacagc cgtgggatat
     3421 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3481 gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt attctggagg
     3541 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3601 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3661 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3721 ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     3781 ctgaacgtac cgacgatgat ttatacgata ctgtgattgg ctaccgtggt ggcaactgga
     3841 tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
     3901 cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt gccgcctatc
     3961 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg
     4021 aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4081 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4141 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4201 ttgcgccgcg cggcattgtg atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa
     4261 aatggaagct cacccaggac tccagcctgt tgcatcagca cgtcttaaag gcgctgccta
     4321 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4381 ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4441 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4501 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4561 agctgaatcg ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa
     4621 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4681 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4741 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4801 ctaaattttg ccaatttggt aaaacagttg catcacaaca ggagatagca atgacgttac
     4861 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     4921 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     4981 tgaaaccggc accggaagtg cgtgaattct ggggctggtg gatggagctg gaagcgcagg
     5041 aatcccgttt tacctacagc taccagtttg gtctgttcga taaagcaggc gactggaaga
     5101 gtgttccggt aaaagatact gaagtggttg aacgactgga gcacaccctg cgtgagtttc
     5161 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt
     5221 ttcgtgacga gccggtgaag ttaacggcgt gagtgaaatg tgccggatgc atcacatccg
     5281 gcaatatcta ttaaaactga tacgtcatcc caatcgcgac aatatcatca ttattaatat
     5341 tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaatgct gacatatttt
     5401 tgttgaaata atacgtagca ccgacgtcga tataattaac cagatcttca ccaccgatgc
     5461 cctcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5521 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5581 gcgtcatttt gcgagtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5641 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5701 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5761 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     5821 ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt
     5881 tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg
     5941 agtcgccacc aaattccgga aacatatcgg tccaggcttc cacgtcatac aacgcgccca
     6001 ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac
     6061 gcgttttttg ctgtgcagta tcactctctg ctttattacc ggcaaactct gcttcccaac
     6121 gaccataacc agtcagttga tcgttaattt gtgtttcgcc tttaaaacca aaacggatat
     6181 aactctggtc gccatcttta ctgtcgttat cactcatata atgcatggct ttaactttgc
     6241 catagacatc cagtttatta ccgtctttgt tatatatttc tgcggcctgt acggatgcag
     6301 atgccacaat gcccatcacc actaatgcca gagtgctctt tttcattttc attcctgatt
     6361 ttaattaacg cgcgaatatt cagcgggaga gccccgttga aaacaggaaa gtttttaacc
     6421 tgagattgtt aaagatatat tacagattaa taatattctt aaaatgtggt aatttattaa
     6481 atctgtaata aaagcgtaaa caactgccgc taggcttgct gatcccgcgc aacaaaacgc
     6541 catgctttgc tcgcagatgg ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat
     6601 atcatttttc ctaaaattga atggcagaga atcatgagtg acagccagac gctggtggta
     6661 aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc tgaaccgtgc ccatatcgtt
     6721 gaacttgttc gccagtgcgc gcagttacat gccgccgggc atcggattgt tattgtgacg
     6781 tcgggcgcga tcgccgccgg acgtgagcac ttgggttacc cggaactgcc agcgactatc
     6841 gcctcgaaac aactgctggc agcggtaggg cagagtcgac tgattcaact gtgggaacag
     6901 ctgttttcga tttatggcat tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa
     6961 gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg cgttgctcga taacaatatc
     7021 gttccggtaa tcaatgagaa cgatgctgtc gctacggcag agattaaagt cggcgataac
     7081 gataaccttt ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt gctgctgacc
     7141 gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga actgattaaa
     7201 gatgtttacg gcattgatga cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc
     7261 ggaactggcg gcatgagtac caaattgcag gccgctgacg tggcttgccg tgcgggtatc
     7321 gacaccatta ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt
     7381 tccgtcggta cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa acgctggatt
     7441 ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag gggcaactgc cgccattctg
     7501 gaacgcggca gctccctgtt gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt
     7561 ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata tcgcccacgg tgtcagtcgt
     7621 tacaacagcg atgcattacg ccgtattgcc ggacaccact cgcaagaaat tgatgcaata
     7681 ctgggatatg aatacggccc ggttgccgtt caccgtgatg acatgattac ccgttaagga
     7741 gctggccgat gctggaacaa atgggcattg ccgcgaagca agcctcgtat aaattagcgc
     7801 aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac
     7861 aaagcgaaat cattctcaac gctaacgccc aggatgttgc tgacgcgcga gccaatggcc
     7921 ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc acggctgaaa ggcattgccg
     7981 acgatgtacg tcaggtgtgc aacctcgccg atccggtggg gcaggtaatc gatggcggcg
     8041 tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc gctgggagtt attggcgtga
     8101 tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta
     8161 atgcggtgat cctgcgcggt ggcaaagaaa cgtgtcgcac taacgctgca acggtggcgg
     8221 tgattcagga cgccctgaaa tcctgcggct taccggcggg tgccgtgcag gcgattgata
     8281 atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga taaatacatc gacatgctga
     8341 tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga acagtcgaca atcccggtga
     8401 tcacaggtgg tataggcgta tgccatattt acgttgatga aagtgtagag atcgctgaag
     8461 cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag cacatgtaat acggttgaaa
     8521 cgttgctggt gaataaaaac atcgccgata gcttcctgcc cgcattaagc aaacaaatgg
     8581 cggaaagcgg cgtgacatta cacgcagatg cagctgcact ggcgcagttg caggcaggcc
     8641 ctgcgaaggt ggttgctgtt aaagccgaag agtatgacga tgagtttctg tcattagatt
     8701 tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc ccatattcgt gaacacggca
     8761 cacaacactc cgatgcgatc ctgacctgcg atatgcgcaa cgcccagcgt tttgttaacg
     8821 aagtggattc gtccgctgtt tacgttaacg cctctacgcg ttttaccgac ggcggccagt
     8881 ttggtctggg tgcggaagtg gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg
     8941 ggctggaagc actgaccact tacaagtgga tcggcattgg tgattacacc attcgtgcgt
     9001 aaataaaacc gggtgatgca aaagtagcca tttgattcac aaggccattg acgcatcgcc
     9061 cggttagttt taaccttgtc caccgtgatt cacgttcgtg aacatgtcct ttcagggccg
     9121 atatagctca gttggtagag cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta
     9181 ttatcggcac catttaaatc aataagttac acatcattag tacgttcctt atttcttgac
     9241 tgggacaaat ttgggaccga tgggttcaga atcgagtcta tttgccgtgc gtgttcggta
     9301 aggtgattag gtgcgaggtg agcatatcga cgaaccattt cgatagactc ccagcctccc
     9361 atttcctgta acactgataa cgggactccg gcttgaacca gccaacttgc ccaggtgtgt
     9421 ctcaagtcgt gaaatctgaa atcatcaata cctgctcgtc tcagcgccgc tttccaggct
     9481 gtgtttgcgt cataccgcat cttcctgact gttggcgctt tcgttccgtc tggtttggta
     9541 cagctttcct tatacacaaa tacccaacgg tgatgattcc cgatttgttt tttcaatacg
     9601 cgacatgcag tatcattcag cgcaacgcca attgcgcggt ttgatttact ctcttccggg
     9661 tttatccatg ccacccggcg ctgcatgtct atttgttgcc attcaaggtt gatgatgttc
     9721 gagcgtctta agcctgttgc aagtgcaaat tcaacaacag actttaatgg ctccggacat
     9781 tcatcaatca gcctttgtgc ttcatgaggc tccagccagc ggatccgttt attctttggt
     9841 tgaggcactt taataattgg tgccttatcc agcattttcc attcacgctc tgcggctctt
     9901 agcagggcct ttataaatga aagatgcgta gccttcgttg caacggacgc tggttttggc
     9961 gtgtattctg gaacaggttt cccttttttt ctgcatgctt ctgccctgag tttccagttt
    10021 tcctcatgac gccggttcgt cattttctgc attgctgaat aaatttttga ttcagtaatg
    10081 tctcttagtt gcattcctgc gaaatgttga agccagaatc cgatccggct tttgtcatcg
    10141 tccagtgatt ttttatgtgc tttctcttca agccacctga cacacgcttc ctcgaacgtt
    10201 atatcaggaa tttcaccaag tttgctgacc cgccatgctt cagcctttag cttgtcatgg
    10261 agttctgtcg cctgcctttt gtcctttgtt ccaagagact gtttaaatct tttaccgttc
    10321 ggcaatgtga aactggcgta ccatatttca cctctgcgga agagtgacat tttctttcct
    10381 ctgttatgcc atcacccgcg ctcacctgga cagtatgcag cggagactga agcgccgcaa
    10441 tgcaggcttg tcgtgttgtg aggtaaggag atttattctt agtgggatct ttgcgtgttg
    10501 cctgcagacg ccctgtgcgt atccagttaa tggcagtcgg tctggatatc ttgagaaaat
    10561 gacaggcctc atctagcgtg aggctgtatg gttccattat ttcacctctt gctgtgatat
    10621 tgttgaaaaa tgaatgccag ctcgttgttg ccagacgatc caaccgagag tcatatccca
    10681 tgccatgtat tcgttatcgc cgttttttgc tctccgacta tctactaagc caccgaaatg
    10741 tttttccata aataattcat aggattcgcg ttcatctggc tct
//
LOCUS       DAJVTY010000140        10759 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVTY010000140 DAJVTY010000000
VERSION     DAJVTY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.140"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(88..1947)
                     /gene="gss"
                     /locus_tag="OQQ66_003218"
     CDS             complement(88..1947)
                     /gene="gss"
                     /locus_tag="OQQ66_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1906037.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2152..3018
                     /gene="yghU"
                     /locus_tag="OQQ66_003219"
     CDS             2152..3018
                     /gene="yghU"
                     /locus_tag="OQQ66_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1906038.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERQDAS
                     DFETNTEDKRQG"
     gene            complement(3141..3389)
                     /gene="hybG"
                     /locus_tag="OQQ66_003220"
     CDS             complement(3141..3389)
                     /gene="hybG"
                     /locus_tag="OQQ66_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1906039.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3402..3743)
                     /gene="hypA"
                     /locus_tag="OQQ66_003221"
     CDS             complement(3402..3743)
                     /gene="hypA"
                     /locus_tag="OQQ66_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1906040.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3736..4224)
                     /gene="hybE"
                     /locus_tag="OQQ66_003222"
     CDS             complement(3736..4224)
                     /gene="hybE"
                     /locus_tag="OQQ66_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1906041.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4217..4711)
                     /locus_tag="OQQ66_003223"
     CDS             complement(4217..4711)
                     /locus_tag="OQQ66_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1906042.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4711..6414)
                     /gene="hybC"
                     /locus_tag="OQQ66_003224"
     CDS             complement(4711..6414)
                     /gene="hybC"
                     /locus_tag="OQQ66_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1906043.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6411..7589)
                     /gene="hybB"
                     /locus_tag="OQQ66_003225"
     CDS             complement(6411..7589)
                     /gene="hybB"
                     /locus_tag="OQQ66_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1906044.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7579..8565)
                     /gene="hybA"
                     /locus_tag="OQQ66_003226"
     CDS             complement(7579..8565)
                     /gene="hybA"
                     /locus_tag="OQQ66_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1906045.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8568..9686)
                     /gene="hybO"
                     /locus_tag="OQQ66_003227"
     CDS             complement(8568..9686)
                     /gene="hybO"
                     /locus_tag="OQQ66_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1906046.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9875..10162)
                     /locus_tag="OQQ66_003228"
     CDS             complement(9875..10162)
                     /locus_tag="OQQ66_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1906047.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10281..10759)
                     /locus_tag="OQQ66_003229"
                     /pseudo
     CDS             complement(10281..10759)
                     /locus_tag="OQQ66_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2602 a         2970 c         2676 g         2511 t
ORIGIN      
        1 atattagaac atataaatat tgaatatttt gattaatccc cgtactgatt attcttcata
       61 atcagtacgg ggttgctaca acatgtatta ctttttcacc acaattaacg gttcaatatc
      121 actctctttt ttgatgacca gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc
      181 gcccacggtg aaggtacata cctgaatgta tttaccgtcc actttcggca aacaccacag
      241 ttgctgatag atgtttttct gctcggcaaa tttaccgctg gttttgtcca gcacctcttc
      301 atgatggctg accaggtcga tattgctacc acagcgaccg gcaatcggtt tcactgcgta
      361 acctgttttc accaattcat cattaacagt gaaatcggta tccagcaggt aacgatggtg
      421 cgggaacagc gaccagagga tcggcagaat cgctttgttg ccggggatca ccgtccacag
      481 cggctcaaag actagcactt ccgggcgcag caatacgtcg ataagacgca cttcgttttg
      541 cggatgaccg gtacggattg gcaccgcagc aaactcacgg tcgctaactt cacgaatctg
      601 atcaaacgcg gtttcccacg cccaggtttt ccacacgcag ttaaccagtc gcccttcccc
      661 atcaatcagt tgcccggcag catcccagcc cagttcatcc aatccacgca agatacgcgt
      721 ttcaaagccc gcctggtgca gcgcctgctc cataaactgc gcgtgatagt tttcctcgat
      781 atctttgtcc tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc
      841 caattcgtta atcagccctt ccgccggatt gaagccgttg cctttatagc cctgctccgc
      901 ccaacgttcg aggatcaagc ccgcttcggt atgacaggag gcggagtcgg cgttgtactc
      961 gtaaaccttc aggccacgct catccatgca gaaatccata cgaccagtga tcatatggtg
     1021 acggcgacgc tgccaggaga gacgcaaacg tggccagagg attttcggaa tgtcgaacag
     1081 cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg tgaagataca tcaggtgcag
     1141 ctcgttggtg gctttaatta gctcctgctc ggcactctcg gtaatggtgt agtaatgata
     1201 aggatcctga ttgatcacct gaccgttggc ctgcacatag gcgttttgca gcggatcttt
     1261 ttcatccagc catttaccgt caaactggcc tttgttttcc aggcgcgctc cgctgatttt
     1321 cagcagctcg cctgcaattt ccggctgcgg taagctgtat tcagtatctt ccgtctggat
     1381 catccagccc agaatggtgg tgtcatcaaa agtgtctttc agggtatagc agccgttttc
     1441 gaccaccatc tccagctcac gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac
     1501 gttctgttcc gcaatacgga ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc
     1561 agtgtcttta aattcaccgc ctttatccca gataagaagc gcacccgcga ccggcgcacg
     1621 cggcgagccg ttaggaaatg cctgcaatgg caggatgttg tcattaacca cttctcgcag
     1681 gaagcgcagc gagaaaatct cccacgccat acccacgtca gtaaagacca caccgtaatt
     1741 cagaaagaga aaacggcgag caaattcaac gcattgccac ttgtggccca tatattcgtc
     1801 gtcgatatag ctacggaata cggcgtcatc ttcgtattcc tgcggatcga gagaactgta
     1861 atctgaagag tagattgcta ccccacctgg ggcgtagccc aataatgtcc cgaacggggc
     1921 atcctggctg gtcgttcctt tgctcatcac tttaccttta acaatacaac ctaagcagtt
     1981 ggcgtaattg ttgtgctctg attacctgct cagcgacatt aaccggacag aggtcagact
     2041 aatcatacac ctcatcgcag cggctggcgc aaagggttaa aaaattcaca ttctgtacag
     2101 caagtgacct gccacactgc ttcaacacta ccactcagaa ggcaactcac tatgacagac
     2161 aatacttatc agcccgcgaa agtctggacg tgggataaat ccgctggcgg cgcgttcgcc
     2221 aatattaatc gcccggtttc tggtccgacg catgaaaaaa cgctgcccgt tggcaaacac
     2281 ccattgcaac tttattcgct gggaacgccg aacggtcaga aagtaacgat tatgcttgag
     2341 gagctgctgg cgctgggcgt tactggtgca gagtacgacg cctggctgat tcgtattggc
     2401 gatggcgatc aattctccag cggctttgtc gaagtgaacc caaactcgaa gatcccggcg
     2461 ctgcgcgatc atacgcataa tccgccgatc tgtgtgtttg aatctggttc gatcctgctt
     2521 tatctggcgg agaaatttgg ctacttcctg ccgcaggatt tggcaaagcg tactgaaacg
     2581 atgaactggc tgttctggtt acagggcgcg gcaccgttcc tcggcggtgg ttttggtcac
     2641 ttttaccatt acgcaccggt aaagattgag tacgccatca accgctttac catggaagcc
     2701 aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat
     2761 gagtacacca ttgcggatat ggcgatttgg ccgtggtttg gcaacgtggt gttaggtggt
     2821 gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata agcatgtaca acgctgggcg
     2881 aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta ttgttaaccg caccaacgga
     2941 ccgctgaatg agcagttgca tgagcgccag gacgccagtg atttcgagac gaatacggaa
     3001 gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa gccatccagg ccggataagg
     3061 cgttcacgcc gcatccggca atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc
     3121 aggcctacga aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg
     3181 tctaatgtgg ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc
     3241 agcacccact ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg
     3301 tcgcgcttga taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc
     3361 agcacctggc ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt
     3421 ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac
     3481 tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg
     3541 ggtttataga cgatatgtaa atcgcaccct tgagccaccg ttccgtggca gacaatttca
     3601 aaactaaaac ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg
     3661 gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc
     3721 tggcaaagcg acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg
     3781 ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc
     3841 atccgtcagg cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga
     3901 acaggagagg tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata
     3961 cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc
     4021 ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc
     4081 gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg
     4141 catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg
     4201 gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt
     4261 cgcgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg
     4321 ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg
     4381 gaaactcacc ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag
     4441 agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg
     4501 gcgtgttctt tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt
     4561 cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa
     4621 tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa
     4681 tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt
     4741 gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt
     4801 acgcaccact tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc
     4861 gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac
     4921 tgcctggtag ttactgataa taccgtcttt aataaccatc cagtgagaga gcataccgcg
     4981 cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac
     5041 aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt gattttgcag
     5101 gatatcctgc aattcgcagc agtgaacggt acgaccaata atacggccca gcgtggagtg
     5161 cagctgcgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt
     5221 cagtttgttc tgggtagatt cgcgacctgc cgccagtttc accagcatat tagccagcgg
     5281 acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc
     5341 gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggtgcct gcggcgcttc
     5401 gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga
     5461 atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa
     5521 caggaagctg ccgtttttac tgtcggtcgg gaattccggc acgctcaggt agttcaccgc
     5581 acctttaccg cgcgtcagcc attccgggta gaacgcggca ataactgcgg tatcaacctt
     5641 ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa gacttgatgt acatcaggcg
     5701 ctcaaggttc agcacgccca aaccgtcgag gttgatcggg ttcgcgacac cacctaccgc
     5761 caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg
     5821 ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag
     5881 tttcatcgcc gggtgacccc agtagccatt ggcgaaaata cccaactgac cgctggcaac
     5941 cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt
     6001 cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat
     6061 gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg
     6121 cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc
     6181 actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg
     6241 ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg
     6301 ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat
     6361 gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg
     6421 ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga
     6481 atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct
     6541 tccctggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc
     6601 agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa
     6661 tcattacgca gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca
     6721 atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag
     6781 ataagctcgc caaagcgcag cacgataaaa atcgccagca acacactgat ggtgttggtc
     6841 agcttgacga acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc
     6901 gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc
     6961 ggcaacattt catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt
     7021 gaccccattg aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac
     7081 atcaccttgt tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga
     7141 gcaaactcca gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt
     7201 accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc
     7261 acgtcgatag tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc
     7321 ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc
     7381 cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac
     7441 gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc
     7501 aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg
     7561 ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt
     7621 ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc
     7681 tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt
     7741 cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc
     7801 ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc
     7861 agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt
     7921 tttcgcctcc gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac
     7981 ttctacgcag ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag
     8041 ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg
     8101 acaggcgacc atgcagtaac ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc
     8161 ggttttcgga tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac
     8221 gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac
     8281 ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt
     8341 gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt
     8401 ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc
     8461 cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag
     8521 caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt
     8581 gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca
     8641 ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttaccg
     8701 ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca
     8761 agctgatgga tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga
     8821 ccaatcgcta ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg
     8881 taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca
     8941 tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg
     9001 tgaatcagac ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg
     9061 ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc
     9121 ggaatattga taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg
     9181 ttaactccag ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct
     9241 gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa
     9301 taaataccgt tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg
     9361 tacttctcaa gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc
     9421 acttcgtgat actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt
     9481 gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc
     9541 ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc
     9601 atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa
     9661 tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata
     9721 ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta
     9781 tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg
     9841 taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca
     9901 taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt
     9961 tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc
    10021 cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac
    10081 agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc
    10141 ctttaccaaa atggttattc atcgttcacc tcattcgcta tatattatca tatatagcaa
    10201 tattttaggc aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa
    10261 tcccgtaaga gataaaccca tcaggagaga tatttatcga accatttcag cgtcctttgc
    10321 caggcaagat cggcggcaga tttgtcataa cgaggcgtgg aatcattatg gaatccgtga
    10381 ttaacccccg gatagatata cgcctcatag accttattat tggctttcaa cgccgcctca
    10441 taagcgggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc
    10501 gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag
    10561 gccagttccg gatacgcgac agccgccgcg ttcgatacac caccgccata gcaaaatccg
    10621 gtaataccca cttatgcctg tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa
    10681 gaaatcattc atcagtttgg ttggatcaac ctgctgttgc agctcacgac ctttatcatc
    10741 atttcccgga taacctcca
//
LOCUS       DAJVTY010000141        10663 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVTY010000141 DAJVTY010000000
VERSION     DAJVTY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.141"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OQQ66_003230"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OQQ66_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1906048.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OQQ66_003231"
     CDS             complement(1606..1929)
                     /locus_tag="OQQ66_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1906049.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OQQ66_003232"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OQQ66_003232"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1906050.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OQQ66_003233"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OQQ66_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1906051.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPSALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OQQ66_003234"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OQQ66_003234"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1906052.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OQQ66_003235"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OQQ66_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1906053.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OQQ66_003236"
     CDS             7529..8566
                     /locus_tag="OQQ66_003236"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1906054.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OQQ66_003237"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OQQ66_003237"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1906055.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OQQ66_003238"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OQQ66_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1906056.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OQQ66_003239"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OQQ66_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1906057.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2655 a         2704 c         2740 g         2564 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgac tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgcataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggaaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccacgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt tttggtagac agt
//
LOCUS       DAJVTY010000142        10360 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVTY010000142 DAJVTY010000000
VERSION     DAJVTY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.142"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(32..1027)
                     /gene="gapA"
                     /locus_tag="OQQ66_003240"
     CDS             complement(32..1027)
                     /gene="gapA"
                     /locus_tag="OQQ66_003240"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1906058.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1369..1782
                     /gene="msrB"
                     /locus_tag="OQQ66_003241"
     CDS             1369..1782
                     /gene="msrB"
                     /locus_tag="OQQ66_003241"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1906059.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1824..2096
                     /locus_tag="OQQ66_003242"
     CDS             1824..2096
                     /locus_tag="OQQ66_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1906060.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2257..2898)
                     /gene="pncA"
                     /locus_tag="OQQ66_003243"
     CDS             complement(2257..2898)
                     /gene="pncA"
                     /locus_tag="OQQ66_003243"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1906061.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2909..3925)
                     /gene="ansA"
                     /locus_tag="OQQ66_003244"
     CDS             complement(2909..3925)
                     /gene="ansA"
                     /locus_tag="OQQ66_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1906062.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4092..5948)
                     /gene="sppA"
                     /locus_tag="OQQ66_003245"
     CDS             complement(4092..5948)
                     /gene="sppA"
                     /locus_tag="OQQ66_003245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1906063.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6109..6660
                     /locus_tag="OQQ66_003246"
     CDS             6109..6660
                     /locus_tag="OQQ66_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1906064.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6777..7820
                     /gene="selD"
                     /locus_tag="OQQ66_003247"
     CDS             6777..7820
                     /gene="selD"
                     /locus_tag="OQQ66_003247"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1906065.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7825..9786
                     /gene="topB"
                     /locus_tag="OQQ66_003248"
     CDS             7825..9786
                     /gene="topB"
                     /locus_tag="OQQ66_003248"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1906066.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9913..>10360
                     /locus_tag="OQQ66_003249"
     CDS             9913..>10360
                     /locus_tag="OQQ66_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906067.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLITPYRFYHKQWSETLEFWPRKPEPGKDTFQ"
BASE COUNT         2607 a         2532 c         2734 g         2487 t
ORIGIN      
        1 cgctcttttt agatcacagt ttcatctcaa cttatttgga gatgtgagcg atcaggtcca
       61 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
      121 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
      181 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
      241 cggctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
      301 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
      361 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
      421 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
      481 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
      541 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
      601 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
      661 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
      721 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac accaacttcg tcccatttca
      781 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
      841 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
      901 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
      961 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     1021 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     1081 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     1141 aagcattatg cgactgactc gcctcactct tcctttgggc ttgaaaccac atggggtcgg
     1201 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     1261 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     1321 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat ggctaataaa
     1381 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     1441 catgggacag agccgccatt tacggggcgt ttactgcata acaagcgtga cggcgtatat
     1501 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     1561 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     1621 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     1681 gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc tgcttcttta
     1741 cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     1801 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     1861 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat ggcgttgcgt
     1921 tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa gcccgccata
     1981 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     2041 agcagttaaa agaagatttt ggtatctcag caaagcctat tgctatgttt aagtgaatga
     2101 gttatctagt atttaagcct gttattaagg gagggcaaat atacttgttc ggacgtcgtg
     2161 ttttagctgc aagtaggtta aatgcaacac tgacaagtat tatctggcct acaatgtcat
     2221 tgaggaaatg taggccagag atgaattcaa ctaatattac ccctgtgtct cttcccagtc
     2281 tgccagcgta tatagcgttg ccccagctgc tgacatctcc ataaacgcgt gtgcactgtc
     2341 ctggggctgg atattcacgc cacgacaacc atcggtaatc acgttcacct tataacctaa
     2401 ctgtaacgcg tccagcacgg taaacttcac gcaatagtca gtagccaggc ccataacgat
     2461 caattcatcg atttcatgat cgcgtaacca gtcatcgaga gaggttttct gccgacggcc
     2521 gttatcaaaa aaggcactgt aactgtcaac taaaggattt tcgcctttat ggaacaccgc
     2581 tgcgatcgct ttttggttca gtaacggatg taattgtgcg ccttcactgt tctgcacaca
     2641 gtgatctggc cagaaggttt gtggcaaacc gtcgagttgg cctggcgtat aaggctctac
     2701 accgtgctga ctggcaaaac tgccgtgatt cgccggatgc cagtcctggc tggcgataac
     2761 cgcttctccg cgcgactggc accagtcaat aaggcggtta gcaacctcca ccgtactgtc
     2821 accttccggt acggcgagcg cgccaccagc acagaaatca ttttgtaaat cgaccagtaa
     2881 cagggcgcga tggggcattg cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt
     2941 tttggctcat ggccttgcga atggtttcag tatccagttc ctggctcagc aggtaatgca
     3001 gtttggttag cgtggcttct acagtcatat ctgcaccgcc aataacgccg gcatgggcga
     3061 gggcgttacc ggtggcataa ccacccatat tcactttacc ggacatacat tgtgtcaggt
     3121 tgaccaccac aataccgcga tcgctggctt cttgtaattc ctgcaggaag gctttgtttt
     3181 gtggcgcatt acccacgcca taggagcgca gaatcaatgc tttcactggt tggcgcagaa
     3241 aattgcgcac gacatcagca gaaatccccg gataaatcgt cactacgcca attggttgtg
     3301 gcgtgattgg atgaacgatt aattcccctt caccgtgcgg ggcgggtggc gtattcaaac
     3361 gacgaatatg gataccagct tccagtaacg gaggaaggtt tggagaggca aacgcatcaa
     3421 aaccatcggc atgggctttg gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga
     3481 gcgttacttc gttgatcgga taattcgccg caacgtacaa cgcattcagc agattaattt
     3541 gtccgtcaga gcgtaactca gccagcggga tttgtgaccc tgtcacaatg accggtttac
     3601 cgagattctc gagcatgaac gacagcgcag aggcggtata cgccatcgtg tcggtaccgt
     3661 gtagaatgac aaaaccatca tagtcgtcat agtgcgcttt aatatcttca gcaatatgct
     3721 gccagtcatc cggcgtcata tcagaagaat ccatcaacgg cgtatattca tgaatggtga
     3781 aatctggcat ctccgggcga tggaattccg gcatcagcgc cagttggcgt tgtagatgac
     3841 ctgacaccgg aatataaccc tgctcggaac gctgcatccc gatggtcccg cccgtgtagg
     3901 cgacgtaaat tgatttcttt tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt
     3961 atatacgtga ggcaggagga gcgcacctga tactatgcgg ataaggaaat atggggcgag
     4021 acagcgggaa gaggattcgt cgaggtaaaa aataatgtgg ccgacgatct gtcggccact
     4081 cagtacaaga cttaacgcac gttggcgcag gtcaggcaaa acgcataacg gttttgtggg
     4141 tcgttaaacg cggccagctt gtcgctttca cttttaacgg tagaggctac cgaggcaagc
     4201 ggtgcaggta acatggcctg gaacgcatct ggcaacattg cccggacaga accagacatg
     4261 ttgtccatca ctttgtcgaa gaaggtcggt tcatcaacgt agtattccag atgccactgt
     4321 ttcacttttg cgagctctgc tgctttggca accgcatcat cgaaatcccc gagactatcg
     4381 accagcccgt tagcttttgc atcctgaccg gtccagacgt ggccctgggc gattttatca
     4441 atttgctccg gcgtcgaatg acgcgcatca gcaaccagcg tgataaagcg tttatagcca
     4501 ttctcaatgc ttaattgcat catctgctgc gcttccggcg gcagtgccct ggtgatagaa
     4561 acatccgcca gcggtgaagt tgagacacca tcagtatgaa caccaatcga atccagacta
     4621 ttttctacgg tggtgatcac gccgaagata ccgatagaac cggtcagggt gctggggtta
     4681 gccacaatgt aattagctgg cgtggaaatc cagtaaccac cagatgccgc catgccgccc
     4741 atcgatacaa ccacaggctt acccgctgcc cgggctgctg ccagttcagc gcgaatcact
     4801 tcagacgcgg taacgctgcc gcctgggcta ttaacacgca ggacaatcgc tttcactttc
     4861 gggtcaaggc gagcgtcgcg gatttgtgcc gcagtggtat caccgccaac attcccctga
     4921 gtttcctcgc catccataat tgcgccatta gcgaagacga caccgatgct gtcaccggta
     4981 tctgccggcg ttttcaatgc gtaatcgtaa taactgatgg cgcgataatt tttatcagtc
     5041 ttactccagc cgaactcttt ggtcagagct ttttcgattt cggcactcga tgccagtgca
     5101 tcgaccagct tgttttccag tgcatatttc gcggtatcgc caccggtttt ggttaaaccc
     5161 tcaagcaacc cttgcgcgct agggaatacc tgctgagcag ggatctgccg gttagcggca
     5221 acagtattca gatagttttg ccacagctca ccaatccagc ggctgtcagc ttcgcgggct
     5281 gccggtgaca tatcatcacg aataaacggt tcaacggcag atttatacgt acccacgcgg
     5341 aacacatggg tggaaacttt cagcttatcc agcaacgatt tgtagtacag accgttggta
     5401 gcaaaaccgt gcagatcaac cacgccttgc ggtgacagcc aaattttatt ggcgaaactg
     5461 gcgagataat attgcccctg gctgtagttc tcgccaacgg cataaaccgg tttcccgctg
     5521 tcacgaaact ctttcagggc tttgccgatg tactgcatag acggctggtc gccgcctgcg
     5581 aagtttttca gatccatcac gataccggtg atattgtggt cgtccttcgc ctggcgaatg
     5641 gtgttgacga tatcaaacag tgagttttcc tgcagacgat cggaactggc accaagcagc
     5701 tggcggctta atttactaaa ccgctgagaa ctgtcgggtt tatcgacgat cacaccggaa
     5761 atgtccagca gcagtgcgcc acgactggcc gtttctttcg aatcaccacc actgacctgc
     5821 atccagatcc ccacaccaac cagtacgagg aaaataaaga acaggttaag taccatttca
     5881 cggacgaaat tcagcagacg ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg
     5941 gttcgcatgt attctcccaa cttaaggtca cacctgtcac aggcgcaata gatgtggtta
     6001 tcgtaatgac ccaaccgaca cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc
     6061 actctcgtgt taattttgtg aataaatatc acgacaggag ttaatcaaat ggatgcactc
     6121 gaactattga tcaatcgccg tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa
     6181 caactgcaaa acatcctgcg tgcgggtatg cgtgcgccgg accataagtc catgcaaccg
     6241 tggcattttt ttgtgattga aggggaaggg cgcgagcgtt tcagcgccgt actggaacag
     6301 ggggcgattg ctgccggtag tgatgacaaa gctatcgaca aagcccgtaa tgcgccgttc
     6361 cgcgcaccgc tcatcatcac ggtggtggcg aaatgcgaag aaaatcataa agtcccgcga
     6421 tgggaacagg aaatgtctgc cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc
     6481 cagggatttg gcggcatctg gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa
     6541 gcattcggtt gccgtgagca ggataaaatt gtcggttttc tctacctcgg cacgccgcag
     6601 ttgaaagcat ctacgtcgat taacgtcccg gacccgacgc cgtttgtaac ttatttctga
     6661 taattgtcgc gaaactgtct ggattgtgag caaaggcgca ggaattcaga cactctcact
     6721 tatcacttca cggaatgagg gttaccatac ccggattgca ttgacaggag atgtccatga
     6781 gcgagaactc gattcgtttg acccaataca gccacggagc tggttgcggc tgtaaaattt
     6841 ccccaaaagt gttggaaacc atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt
     6901 tgcttgtggg taatgagacc cgcgacgatg cggcggtgta cgatctgggc aatggcacca
     6961 gcgttatcag taccaccgac ttctttatgc cgatcgttga taatcctttc gattttggcc
     7021 gcattgcggc gactaacgcc atcagcgata tcttcgcgat ggggggcaaa ccgattatgg
     7081 cgattgcgat cctcggctgg ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa
     7141 ccgaaggtgg acgctatgca tgccgtcagg cgggtattgc gctggctggc ggtcactcca
     7201 tcgatgcgcc ggagccgatt tttggtctgg cggtaacggg gatcgtaccg accgagcggg
     7261 tgaagaaaaa cagcaccgca caagccggat gcaaactgtt cctgacgaaa ccgctgggga
     7321 tcggcgttct taccacggct gagaaaaaat cattgctgaa accagaacat cagggactag
     7381 cgacggaagt gatgtgccgg atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg
     7441 taaaagcgat gaccgacgtt acgggctttg gtctgctggg ccatttgagc gaaatgtgtc
     7501 agggcgcagg tgtgcaggcg cgcgttgact atgacgcgat cccaaaattg ccgggggttg
     7561 aagagtacat taagttgggc gcagtgcctg gcggcaccga acgtaacttt gccagctacg
     7621 gtcatctgat gggtgaaatg ccgcgtgaag tgcgcgattt gctgtgcgat ccgcaaactt
     7681 ctggcggttt gctgctggcg gtcatgccgg aagcagaaaa tgaggtcaaa gctacagccg
     7741 ccgagtttgg cattgaactg acggcaattg gcgaactggt gccagcgcgc ggcggtcgtg
     7801 ccatggttga gattcgttaa ttcaatgcgg ttgtttattg ccgaaaaacc gagtctggcg
     7861 cgcgccattg ctgatgtcct gcccaaaccg caccggaaag gcgatggctt tatcgagtgc
     7921 ggtaatggtc aggtggtgac ctggtgtatc ggtcacctgc ttgagcaggc gcagccagac
     7981 gcctacgaca gccgctatgc gcgctggaat cttgcggatt tgccgattgt cccggaaaag
     8041 tggcaattac agccccgacc ctccgtgacc aaacaactta acgtcatcaa acgcttcctg
     8101 catgaagcca gcgaaatcgt tcacgccggg gacccggatc gtgaagggca actgctggtg
     8161 gatgaagtgc tggactatct gcaactggca ccggaaaagc gccagcaggt gcagcgttgc
     8221 ttgataaacg acctgaaccc gcaggcggtt gagcgggcga tcgaccgtct tcggtccaac
     8281 agtgagtttg tgccattgtg cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac
     8341 ggcatcaata tgacccgtgc gtataccatt ctcggtcgca atgccggtta tcagggcgta
     8401 ctttccgtgg gacgcgtgca gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag
     8461 attgaaaact tcgtggcgaa agacttcttt gaagtcaaag cacatatcgt gacacctgcc
     8521 gatgagcggt ttaccgctat ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa
     8581 gaagggcgct tgttacatcg tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa
     8641 ccagcaattg tcaccagcta taacgataaa cgggaatcag aatccgcgcc gctgcctttt
     8701 tcgctttcgg cgttgcagat tgaagcggca aaacgctttg gtctgagcgc gcagaacgtg
     8761 cttgatatct gccagaagct gtacgaaacg cacaagctaa tcacttatcc gcgttccgat
     8821 tgtcgctatt tgccagaaga acattttgcc ggacgccacg cggtgatgaa tgccatcagc
     8881 gttcatgcac cagatctgtt gccgcaacca gtggtagatc cagatatacg caaccgctgt
     8941 tgggatgaca aaaaggtcga tgcacaccac gccatcatcc cgaccgcacg gagttctgcg
     9001 atcaacctga cggagaacga agcgaaggtc tataacctga ttgcccgtca gtacttgatg
     9061 cagttctgcc cggatgcggt gttccgcaag tgtgttatcg aactggacat tgccaaaggc
     9121 aaatttgtcg ctaaagcgcg ttttcttgct gaagcaggct ggcgcacgct gttaggcagc
     9181 aaagagcgcg atgaagaaaa cgacggtacg ccattgcctg tggtggcgaa aggcgatgag
     9241 ttgctgtgtg aaaaaggtga agtggtagag cggcaaaccc agccgccgcg ccattttacc
     9301 gatgcaacac tgctttcggc gatgaccggg atcgcgcgct ttgtgcagga caaagatctg
     9361 aaaaagatcc ttcgtgcgac cgatggtctg gggacagaag caacccgtgc cgggattatt
     9421 gaactgctgt tcaagcgtgg ttttctgacc aaaaaagggc gctatatcca ctctaccgac
     9481 gccggaaaag cgttattcca ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg
     9541 cactgggaat cggtgctgac gcaaatcagc gaaaagcagt gtcgctatca ggactttatg
     9601 cagccgctgg tggggacgct atatcagctt attgatcaag ccaaacgtac gccggtgcgg
     9661 cagtttcgcg gcattgtggc tccgggcagt ggtggcagtg ctgataagaa aaaggctgca
     9721 ccgcgtaaac gtagtgcgaa aaaaagtccg ccagcagatg aagtcggaag cggggcgata
     9781 gcgtaagcga gtgaatcttt cgtgctattc gagtcatatt ctgaaatatc cagcgaatca
     9841 agaaaattcg ttggatattt ttttgcatgg ataaaattat cgcctctaaa gtatgtaata
     9901 acagggaatg tgatgaaaaa agttttatta caaaaccatc ctgggagcga gaagtattct
     9961 tttaatggct gggaaatatt taatagtaat tttgaacgga tgattaaaga aaataaggcc
    10021 atgctgcttt gtaagtgggg gttttattta acatgtgttg tcgctgtaat gtttgtgttc
    10081 gcagcgataa catccaacgg tttgaatgaa agaggcctga ttaccgcggg atgctctttt
    10141 ctttatctat taattatgat ggggcttatt gttcgggccg gttttaaagc aaaaaaagaa
    10201 caactgcatt attatcaggc taaaggtatt gagccgctca gtatcgagaa gttacaggcg
    10261 ctacaattga tcacacctta tcgattctat cataagcaat ggtctgaaac gctggagttc
    10321 tggccgcgaa agcctgaacc tggcaaagat accttccaat
//
LOCUS       DAJVTY010000143        10271 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVTY010000143 DAJVTY010000000
VERSION     DAJVTY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.143"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1120)
                     /gene="icd"
                     /locus_tag="OQQ66_003250"
     CDS             complement(<1..1120)
                     /gene="icd"
                     /locus_tag="OQQ66_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1906068.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAA"
     gene            1292..1945
                     /gene="rluE"
                     /locus_tag="OQQ66_003251"
     CDS             1292..1945
                     /gene="rluE"
                     /locus_tag="OQQ66_003251"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1906069.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            1955..2416
                     /gene="nudJ"
                     /locus_tag="OQQ66_003252"
     CDS             1955..2416
                     /gene="nudJ"
                     /locus_tag="OQQ66_003252"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1906070.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2470..3576
                     /gene="mnmA"
                     /locus_tag="OQQ66_003253"
     CDS             2470..3576
                     /gene="mnmA"
                     /locus_tag="OQQ66_003253"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1906071.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCSVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3612..4253
                     /gene="hflD"
                     /locus_tag="OQQ66_003254"
     CDS             3612..4253
                     /gene="hflD"
                     /locus_tag="OQQ66_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1906072.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4257..5627
                     /gene="purB"
                     /locus_tag="OQQ66_003255"
     CDS             4257..5627
                     /gene="purB"
                     /locus_tag="OQQ66_003255"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1906073.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5795..6466
                     /gene="phoP"
                     /locus_tag="OQQ66_003256"
     CDS             5795..6466
                     /gene="phoP"
                     /locus_tag="OQQ66_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1906074.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6466..7926
                     /gene="phoQ"
                     /locus_tag="OQQ66_003257"
     CDS             6466..7926
                     /gene="phoQ"
                     /locus_tag="OQQ66_003257"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1906075.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8002..9123
                     /gene="roxA"
                     /locus_tag="OQQ66_003258"
                     /gene_synonym="ycfD"
     CDS             8002..9123
                     /gene="roxA"
                     /locus_tag="OQQ66_003258"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1906076.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9172..>10271)
                     /gene="pepT"
                     /locus_tag="OQQ66_003259"
     CDS             complement(9172..>10271)
                     /gene="pepT"
                     /locus_tag="OQQ66_003259"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1906077.1"
                     /translation="EMGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSG
                     KNVNPQIVENYRGGDIALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGI
                     AEIMTALAVLQQKNIPHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGEL
                     EFENFNAASVNIKIVGNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFY
                     HLAGMKGTVERADMHYIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSY
                     YNMREKVVEHPHILDIAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYN
                     YHGKHEFVTLEGMEKAVQVIVRIAELTAQRK"
BASE COUNT         2488 a         2655 c         2624 g         2504 t
ORIGIN      
        1 cagccgcttc agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc
       61 ctggatttac tttgtcctga ccggcgtatt tcggcgcagt accgtgggtg gcttcaaaca
      121 gggcgcattc gtcaccgatg tttgctccag gagcgatacc gataccgcca acctgcgctg
      181 ccagggcgtc agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag
      241 ccggacgcag caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct
      301 ctttgccggt gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc
      361 caaactcttc acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca
      421 tgatgttgcc tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga
      481 tcgctgcacg aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac
      541 aatgttccgg gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt
      601 tctcggcgtc ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt
      661 cacggaagat aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat
      721 agtaacgtac cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca
      781 gagagcgaat accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt
      841 cacgaatcag atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg
      901 atttttcacc ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct
      961 cgactgcagc gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac
     1021 cttcaatgta agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga
     1081 tcttcttgcc ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct
     1141 actggtttgc tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct
     1201 ttagagcaat tttttgttaa tgatttgtaa ttggcttgtc aatactaccc tattgtttgc
     1261 cgtcatgaaa gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac
     1321 catgcaaaaa acttctttta gaaatcacct ggttaagcga ttcagctcgc aacgttctac
     1381 caggcgtaaa cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt
     1441 tcttccgcag ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca
     1501 gggcgtttat gcagcaggtc gtcttgaccg cgatagcgaa gggttactgg tgctgaccaa
     1561 taacggcgcg ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta
     1621 tgtgcaggtg gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac
     1681 cttaaatgat ggccctaccc tgcccgccgg cgcggagctg gttgacgaac ctgcgtggtt
     1741 atggccgcgg aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat
     1801 caccttatat gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc
     1861 cacgctgcga ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg
     1921 cgaatggcga gaagcaacag aataaggatg cacaatgttt aaaccacacg ttaccgttgc
     1981 ttgcgtagtg cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc
     2041 gttatggaac caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc
     2101 ccgtgagcta tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca
     2161 tcagtggatt gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga
     2221 gcaaatatgc ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc
     2281 cgaagaaatt ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg
     2341 ttatcaaagc gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac
     2401 aaagggtgtc atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg
     2461 agtgatccaa tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat
     2521 tcctccgttt ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg
     2581 aagaactggg aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat
     2641 gcccaagctg tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag
     2701 tactgggaca acgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat
     2761 ccggatattc tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa
     2821 gatttaggtg ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc
     2881 aagagccgcc tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg
     2941 ctcagccatg agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag
     3001 gtgcgtaaga ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc
     3061 atttgcttca ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggcgcaa
     3121 ccgggcaaaa tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat
     3181 cacactctcg gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa
     3241 ccgtggtatg tggtggacaa agacgtcgaa aacaacattc tggttgttgc gcagggccat
     3301 gaacacccgc ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc
     3361 gaaccattca ccggtactat gcgttgctcg gtaaaaaccc gctatcgcca gaccgatatc
     3421 ccttgcaccg tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt
     3481 gccgccgtga cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc
     3541 ggtattattg agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg
     3601 aagcagtgaa cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc
     3661 agtcggcacg cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac
     3721 acgtctcact caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg
     3781 gtagcgaagc caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca
     3841 gtcgccaggg cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc
     3901 gcaaactctc ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc
     3961 aacgccagct cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta
     4021 tctatgttga tgtgatcagc ccgcttggcc cgcgcattca ggtcaccggt tcccctgctg
     4081 tactgcaaag cccacaagtg ccggcgaaag ttcgcgcaac cctgctggca ggcattcgcg
     4141 ccgccgtgct ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc
     4201 gcctgaccac tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg
     4261 aattatcctc actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg
     4321 cgctgcgcgg gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac
     4381 gttggctgca aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg
     4441 acgcaatcgg ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca
     4501 tcaaaaccat cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag
     4561 aaaaagtggc ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta
     4621 cttcggaaga tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag
     4681 tgatcctgcc gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc
     4741 gcgatatccc gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta
     4801 aagagatggc aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg
     4861 agatcctcgg caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc
     4921 cggaagttga ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcagtgga
     4981 acccgtacac tacccagatt gaaccgcacg actacattgc cgaactgttt gattgcgttg
     5041 cgcgcttcaa caccattctg atcgactttg accgtgacgt ctggggttat atcgccctta
     5101 accacttcaa acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag
     5161 ttaacccgat cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac
     5221 agcatctggc aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg
     5281 tgctgcgtaa cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga
     5341 aaggcgtgag caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact
     5401 gggaagtgct ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt
     5461 acgagaagct gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt
     5521 ttatcgacgg tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg
     5581 caaactacat tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg
     5641 ccggatggcg atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt
     5701 tacctcccct ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac
     5761 actattttaa taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa
     5821 tgcgttgtta cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga
     5881 tgcagaagat gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat
     5941 tgtcgatctc ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa
     6001 tgatgtttca ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga
     6061 agtattaagt gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat
     6121 ggcgcgaatg caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct
     6181 accaccgttt caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa
     6241 actgaccgcg ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt
     6301 gagcaaagat tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac
     6361 cattgatgta ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt
     6421 gattactacc gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg
     6481 cgtctttttt tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta
     6541 ctggtgcttt cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat
     6601 aaaactacgt ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg
     6661 gaaaacaata agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg
     6721 acgctaattt atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg
     6781 atgaagatga tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat
     6841 gttaacgata ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa
     6901 gtgcgagaag atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg
     6961 gcaacatcgc ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta
     7021 aaaagttcct atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta
     7081 gtgatcccgc tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca
     7141 aaagaagtcc gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga
     7201 gaactgacca gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac
     7261 gacaaatacc gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg
     7321 ctgcaaagta cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg
     7381 gtaatgctgg agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc
     7441 agtatgcgcg gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac
     7501 aatctcacct cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat
     7561 atttcgccag agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac
     7621 gtgctggata atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc
     7681 gacgagcatc tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga
     7741 gaggtcattt tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg
     7801 ctggcggtag cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc
     7861 atgctgggcg gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat
     7921 gaataaatat gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat
     7981 accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt
     8041 gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac
     8101 ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg
     8161 gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat
     8221 ctcggtgaaa ccaactggtc attactggta caggcagtga accactggca tgagccgacc
     8281 gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt
     8341 tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc
     8401 attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac
     8461 tgcccacatc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg
     8521 gagcctggtg atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa
     8581 aatgcgatga actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga
     8641 tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt
     8701 ccacctcgcg ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg
     8761 atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc
     8821 cagtcacgtc atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc
     8881 tacgatgcgc tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc
     8941 attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg
     9001 gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat
     9061 ccgtcattcc tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg
     9121 taagtttgga tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg
     9181 ttgcgccgtt aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag
     9241 agtcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg
     9301 taatcccata aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt tcagttccgg
     9361 ttcaatatcg cagtcacgca ttgcctgctg ggcgatatcg agaatatgcg gatgctcaac
     9421 cactttctcg cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg
     9481 atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacatttac gcgcttcaaa
     9541 ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt
     9601 catgcccgcc aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc
     9661 cggaacttcc gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc
     9721 cgtgcccgga tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt
     9781 ttcaaactcc agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa
     9841 ggcatcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac
     9901 gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc
     9961 tgcaatacct gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaatgt
    10021 ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat
    10081 acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc
    10141 gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg
    10201 gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag
    10261 ccccatttct t
//
LOCUS       DAJVTY010000144        10242 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVTY010000144 DAJVTY010000000
VERSION     DAJVTY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.144"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            141..2020
                     /gene="kup"
                     /locus_tag="OQQ66_003260"
                     /pseudo
     CDS             141..2020
                     /gene="kup"
                     /locus_tag="OQQ66_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
     gene            2187..2606
                     /gene="rbsD"
                     /locus_tag="OQQ66_003261"
     CDS             2187..2606
                     /gene="rbsD"
                     /locus_tag="OQQ66_003261"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1906078.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2614..4083
                     /gene="rbsA"
                     /locus_tag="OQQ66_003262"
                     /pseudo
     CDS             2614..4083
                     /gene="rbsA"
                     /locus_tag="OQQ66_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4088..5053
                     /gene="rbsC"
                     /locus_tag="OQQ66_003263"
     CDS             4088..5053
                     /gene="rbsC"
                     /locus_tag="OQQ66_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1906079.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5078..5964
                     /gene="rbsB"
                     /locus_tag="OQQ66_003264"
                     /pseudo
     CDS             5078..5964
                     /gene="rbsB"
                     /locus_tag="OQQ66_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6090..7019
                     /gene="rbsK"
                     /locus_tag="OQQ66_003265"
     CDS             6090..7019
                     /gene="rbsK"
                     /locus_tag="OQQ66_003265"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1906080.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7023..8015
                     /gene="rbsR"
                     /locus_tag="OQQ66_003266"
     CDS             7023..8015
                     /gene="rbsR"
                     /locus_tag="OQQ66_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1906081.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7981..9408)
                     /gene="mdtD"
                     /locus_tag="OQQ66_003267"
     CDS             complement(7981..9408)
                     /gene="mdtD"
                     /locus_tag="OQQ66_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1906082.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9431..10123)
                     /locus_tag="OQQ66_003268"
     CDS             complement(9431..10123)
                     /locus_tag="OQQ66_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1906083.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2494 a         2465 c         2712 g         2571 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcaggatg cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc
      421 cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg
      481 cagctttttc tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga
      541 aggtctggaa atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt
      601 tctcacttta ttatttatga ttcaaaaaca tggcacggct atggtcggta agctgtttgc
      661 gccgatcatg ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc
      721 taacccggaa gtgctgcatg cgctgaatcc aatgtgggcg gtgcatttct tccttgaata
      781 caaaacggtt tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc
      841 gctgtatgct gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt
      901 cgtattacct tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc
      961 ggaagcgatt aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct
     1021 gatcatcgcc gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc
     1081 attgacgcgt caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc
     1141 cgaaatggag tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt
     1201 cgtgattgtg attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc
     1261 ggtgaccgga accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg
     1321 gcactggaat aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc
     1381 attgttcacc gctaacctcg ataaactgct ctccggcggt tggttgccat tgagcctcgg
     1441 tacggtgatg tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg
     1501 gatgcatgaa catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc
     1561 tgttcgcgtg cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc
     1621 gctgatgcat aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct
     1681 gcgtaccgaa gacgcgccat atgtccataa tgtccgtcgg gtacagattg aacaactgtc
     1741 gctcactttc tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga
     1801 agttttccac cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt
     1861 tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa
     1921 gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc
     1981 aaacagggtt atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctc
     2041 cataaggggg cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc
     2101 acatttccgt aacgtcacga tggttttccc aactcagtca ggattaaact gcgggtcagc
     2161 gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt
     2221 catcggtgat ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac
     2281 ccatccccaa aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta
     2341 tgcaggtgct gggcgtcgtt acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag
     2401 agatcaaaca acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc
     2461 aaaaacacca gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac
     2521 aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca
     2581 ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc
     2641 atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg
     2701 ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg
     2761 cttactggta tctatgctcg cgatgccggc acgcttttat ggctggggaa agaaacgaca
     2821 tttaccgggc cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac
     2881 ctgatcccgc agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc
     2941 tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt
     3001 aacctgcgct ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg
     3061 gttgaaatcg ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc
     3121 gatgcgctga ccgataccga aaccgaatcc ctgttccgcg tcatccgcga actaaaatcg
     3181 caaggccgcg gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat
     3241 gccgttaccg tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc
     3301 gaagattcgc tgattgagat gatggtgggt cgcaagctgg aagatcaata tccacacctg
     3361 gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac
     3421 gatgtctctt ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca
     3481 ggtcgtaccg aactgatgaa agtgctctac ggcgcactac cgcgcaccag cggttacgtc
     3541 accctggatg ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt
     3601 gtgtatatct ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag
     3661 aacatgtcgc tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc
     3721 gatgaacagt aggctgtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg
     3781 gaacaggcaa ttggtctgct ttccggtggc aatcagcaaa aagtgttgat ccttgatgaa
     3841 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
     3901 aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg
     3961 agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag
     4021 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
     4081 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
     4141 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
     4201 gaactttttc accatcaata acttattcaa tattctccag caaacctcgg tgaacgccat
     4261 tatggcggtc gggatgacgc tggtgatcct gacttcgggc atcgacttgt ctgtaggttc
     4321 tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct
     4381 ggtggctgtc gctgctgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat
     4441 tgtagcgaaa ggtcgcgttc aggcgtttat cgctacgctg gttatgatgc ttttactgcg
     4501 cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc
     4561 cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat
     4621 catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
     4681 catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa
     4741 aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggaccattga
     4801 agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat
     4861 tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac
     4921 gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc
     4981 ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa
     5041 caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac
     5101 cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac
     5161 catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc
     5221 gcagaaagag gcggataagc ttggctataa cctggtggtg ctggactccc agaacaaccc
     5281 ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat
     5341 taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat
     5401 cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc
     5461 tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg
     5521 tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga
     5581 aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agcagatttt
     5641 gatcgcacta aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag
     5701 gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcgcgcact gcaaactgcc
     5761 ggtaaatcgg atgtgatggt cgtcggattt gacggtacac cggatggcga aaaagcggtg
     5821 aatgatggca aactagcagc gactatcgct cagctacccg atcagattgg cgcgaaaggc
     5881 gtcgaaaccg cagataaagt gctgaaaggc gagaaagttc aggctaagta tccggttgat
     5941 ctgaaactgg ttgttaagca gtagttttaa tcaggttgta tgacctgatg gtgacataaa
     6001 tacgtcatcg acagatgaac gtgtaatata aagaaaagca gggcacgcgc caccctaaca
     6061 cggtggcgca ttttatggac atcccgaata tgcaaaacgc aggcagcctc gttgttcttg
     6121 gcagcattaa tgctgaccac attcttaatc ttcaatcttt tcctactcca ggcgaaaccg
     6181 taaccggtaa ccactatcag gttgcatttg gcggcaaagg cgcgaatcag gctgtggctg
     6241 ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac gggtgatgac agcattggtg
     6301 agagcgttcg ccagcagctc gccactgata acattgatat tactccggtc agcgtgatca
     6361 aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag aatgtcatcg
     6421 gtattcatgc cggcgctaat gctgcccttt ccccggcgct ggtggaagcg caacgtgagc
     6481 gtattgccaa cgcgtcagca ttattaatgc agctggaatc accactcgaa agtgtgatgg
     6541 cagcggcgaa aatcgcccat caaaataaga ctatcgttgc gcttaacccg gctccggctc
     6601 gcgaacttcc tgacgaactg ctggcgctgg tggacattat tacgccaaac gaaacggaag
     6661 cagaaaagct caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag gcggcgcagg
     6721 tactgcatga aaaaggtatc cgtactgtac tgattacttt aggaagtcgt ggtgtatggg
     6781 ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct gtcgatacca
     6841 ttgctgccgg agataccttt aacggcgcgt taatcacggc gttgctggaa gaaaaaccat
     6901 tgccagaggc gattcggttt gcccatgctg cagctgcgat tgccgtaaca cgtaaaggcg
     6961 cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg cagaggtgac
     7021 gcttggctac aatgaaagat gttgcccgcc tggcgggcgt ttctacctca acagtttctc
     7081 acgttatcaa taaagatcgc ttcgtcagtg aagcgattac cgccaaagtt gaagcggcga
     7141 ttaaagaact caattacgcg ccatcagccc tggcgcgtag cctcaaactc aatcaaacac
     7201 ataccattgg catgttgatc actgccagta ccaatccttt ctattcagaa ctggtgcgtg
     7261 gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt cctttgcaat accgaaggcg
     7321 atgaacagcg gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt gatggcttgc
     7381 tgttactgtg caccgaaacg catcaacctt cgcgtgaaat catgcaacgt tatccgacag
     7441 tgcctactgt gatgatggac tgggctccgt tcgatggcga cagcgatctt attcaggata
     7501 actcgttgct gggcggagac ttagcaacgc aatatctgat cgataaaggt catacccgta
     7561 tcgcctgtat taccggtccg ctggataaaa ctccggcgcg cctgcggttg gaaggttatc
     7621 gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc actggtgatt
     7681 ttgaatttaa cggcgggttt gacgccatgc gccaactgtt atcacatccg ctgcgtcctc
     7741 aggccgtctt taccggaaat gacgctatgg ctgttggcgt ttaccaggcg ttatatcagg
     7801 cagagttaca ggttccgcag gatatcgcgg tgattggcta tgacgatatc gaactggcaa
     7861 gctttatgac gccaccatta accactatcc accaaccgaa agatgaactg ggggagctgg
     7921 cgattgatgt actcatccat cggataaccc agccgaccct tcagcaacaa cgattacaac
     7981 ttactccgat tctgatggaa cgcggttcgg cttagattta cgctgtcttt tgatcaaatt
     8041 attaccatcg gttgttttca gaagcatgaa cattgctgct gaagcaacag taataatgcc
     8101 catcgtgata aacgtatagt ggaattgttc gacagtcgtt gtgccttcca tcccttcata
     8161 aacgcgaagg acggccgcac ttacagcaac gcctaaacta atcgacagtt gctgcgtgac
     8221 cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa gtgtgatggt
     8281 attcatcgcg gtaaattgcg tcgacatagc catccctaat ataaacaacg gcaagatcag
     8341 catccatatc gccattgccg gtgattgcaa agagaactga gcgatcatta gcccaataat
     8401 caccgtgatc cccactaacg tatggcgata gcccagacga cgtaagactt gggtaaccat
     8461 cgattttgca ataatggaac ctaacgctgt cggtgccatc atacagccgg caataaacgc
     8521 ctgataacca aatcctacct gtaacatcaa tggcataagg aacggtacac taccggtccc
     8581 cagacgggtt gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt taaataaatc
     8641 taatgaaatt aatgggtttg gcgtgtgtcg cgcatggaga atatagagaa gcagtaaccc
     8701 gatgctggtg acaattaccg tcaaggcaat ccagctggcg acaatctttt ccccgaatag
     8761 ctctattccg cttgagaaga gaacaaggct gaggccaaac agcaaaaagc cagtgatatc
     8821 gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg cgcgcgtaaa gaaggcccgc
     8881 aatacctatg gggatattga ttaaaaatat ccagtgccag gttgccctgg tgaccagcac
     8941 gccgccaaga acggggccta aaattggccc caccagaccc ggcatggcga caaaattcaa
     9001 tacaggaaga agttcattac gaggataagc acgcagtaag gccagccgag caacaggcat
     9061 catcattgcg ccgcctatcc cctgaataac ccggaagaca accagctgtg gtagcgaatt
     9121 agaaagtgcg caggccagag aacccaacgt gaacagactc acggcaaggg taaaaatgcg
     9181 acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga ataaacatcg ccaccgtcag
     9241 cgtataactg atgatggctg attgcatcgc gagaggagaa cgattaaggc tatgagcgat
     9301 tgcgggtaag gcggtattca gaatagtggc atcaagtgcc tgcatgaaga aggccatcgc
     9361 cgcgatccac ggcaaacccg ccatactacg cttcttttta tcgctcattc aatgtcctgt
     9421 tatcgggtta tcacttatca ggtgagcgta gcagcgcctg acaagtttta aatgccgcgt
     9481 cgccatcgct ttggataatc gcatcgacaa tcgcctggtg cagatccagc tttatcactg
     9541 tgtcgctggt aattgacgtg aagtaagtgt gataaaccga atggaatagc gaggcgaatg
     9601 aggtcaaaaa cggattggcg ctcatttcat agatatgctc atgccaggcc atatcgactt
     9661 cgatccagcg ttcacggcga aagttctttt ttaatgccgc catttcggcc attaacgtat
     9721 tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc cagtaggcag gcttgcggct
     9781 ccagacagat acgcataacc agaaagtgat cgatgacctg atgaaagttc tcttctgtca
     9841 tccaccaggt aagcaattcc tgatcaagaa aattccagtt tgattgtggc atgacccggg
     9901 taccaattcg cggtcgcggt aaaaccatcc cttttgccgt taacgttttg accgcttcgc
     9961 gtaccgctgt acgactcact ccaaattgct cgcccagctc aatctcacca ggcaaaatgg
    10021 tgccgggttc atattcacct tttaagatgc gttgcgccag cttctcagcc agaacatacg
    10081 aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg cattactaat cttccttttt
    10141 ctttttattc ctccttagta tgccaccagg aagtgtgatt acggttgcaa aaacggcaaa
    10201 ttgcttgttt tatggcacat taacggggct tttgctgaaa aa
//
LOCUS       DAJVTY010000145        10207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVTY010000145 DAJVTY010000000
VERSION     DAJVTY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.145"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(267..1298)
                     /gene="rsmC"
                     /locus_tag="OQQ66_003269"
     CDS             complement(267..1298)
                     /gene="rsmC"
                     /locus_tag="OQQ66_003269"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1906084.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1401..1814
                     /gene="holD"
                     /locus_tag="OQQ66_003270"
     CDS             1401..1814
                     /gene="holD"
                     /locus_tag="OQQ66_003270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1906085.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1783..2229
                     /gene="rimI"
                     /locus_tag="OQQ66_003271"
     CDS             1783..2229
                     /gene="rimI"
                     /locus_tag="OQQ66_003271"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1906086.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2244..2921
                     /gene="yjjG"
                     /locus_tag="OQQ66_003272"
     CDS             2244..2921
                     /gene="yjjG"
                     /locus_tag="OQQ66_003272"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1906087.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3012..4601
                     /gene="prfC"
                     /locus_tag="OQQ66_003273"
     CDS             3012..4601
                     /gene="prfC"
                     /locus_tag="OQQ66_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1906088.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4994..5599
                     /gene="osmY"
                     /locus_tag="OQQ66_003274"
                     /pseudo
     CDS             4994..5599
                     /gene="osmY"
                     /locus_tag="OQQ66_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            5726..5887
                     /locus_tag="OQQ66_003275"
     CDS             5726..5887
                     /locus_tag="OQQ66_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1906089.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6009..7082
                     /locus_tag="OQQ66_003276"
     CDS             6009..7082
                     /locus_tag="OQQ66_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1906090.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7079..7861
                     /locus_tag="OQQ66_003277"
     CDS             7079..7861
                     /locus_tag="OQQ66_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1906091.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8075..8938)
                     /locus_tag="OQQ66_003278"
     CDS             complement(8075..8938)
                     /locus_tag="OQQ66_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1906092.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8910..>10207)
                     /locus_tag="OQQ66_003279"
     CDS             complement(8910..>10207)
                     /locus_tag="OQQ66_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1906093.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPH
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
BASE COUNT         2409 a         2664 c         2750 g         2384 t
ORIGIN      
        1 taccatcaat tcttaaaaag aattgctacc acggaggcgc attctagtgg ttttcagctt
       61 ttcgtcaata gttaattatc gccggaggtg taattgctgg aaaaatgtcc atcaggaaac
      121 tagcgtgcag gtttggtagg catgcggggg cagatgccag atgcgacgct ggcgcgtctt
      181 atctggccta cgaagggcta acgtgcaggt tttgtaggtc ggataaggcg tttacgccgc
      241 atccggcaag gcttgcggcg ggatacttaa ccttttttcg cctggcgggt cataatggcg
      301 cgatatacct tgaaacgccc ggtttgcgca atcacttcat ggaagccaaa tgtttcatcc
      361 agcacgtccg gataaggcag gaaggcgttc gctacaattc gcagctcgcc gccactatta
      421 agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca
      481 tcgtggaacg gcgggttgga gatgatcata tcaaaacgac ctttcacctc ggaaaagacg
      541 ttgctggcaa agacttcacc ttcaacacag ttggccgcaa gtgttgcgcg gctggcttct
      601 accgctggcg cagagacatc gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag
      661 gcaactgaaa gcacccccgc gccacagccg acatccagca ctttaccttt cgtgtgcgga
      721 gttaacgtcg agagcagcaa ctggctaccg acatccagac cgtcgcggct aaacacgcca
      781 ggcagcgttt tgactgtcag gccatcgacg ctgtattcgc cccagaattt atcggcatca
      841 aataccggct gtttttccag acgaccaaaa tagaggccac agcgacgagc gctgtcgact
      901 ttattcaacg gcgcataatc tgccagcatc tgctcggcgc tgcgcacgcc gctgcggttc
      961 tcgccaacga caaaaatatc tgtccccact ggcagcagag aaagtaaatt catcaactgg
     1021 aactgggctt ccggtttgtt cttcggccag tagtaaatca gcgtatcgca atctgcgacg
     1081 tcatccaccg tggcgaccag actgaaacgg gcgttatccc ccatctggcg gcttaatacc
     1141 tgccagtggt ggaattgctg ggtatgagca cggctggccg cggtatctaa acgcgcgggc
     1201 aggtcatcct gtaagtctcc ggcaaacaga atacggcttt gttcgaaatc atcactgtga
     1261 cgcagcaaga cttcacttgc cggggtaaat gcagacatgg aatgctcctc aattgatact
     1321 ggcggcgatt atagccatat gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct
     1381 gacaacagga gcgattcgct atgacatccc gacgagactg gcagttacag caactgggca
     1441 ttacccagtg gtcgctgcgt cgccctggcg cgttgcaggg ggagattgcc attgcgatcc
     1501 cggcacacgt ccgtctggtg atggtggcaa acgatcttcc cgccctgacc gatcctttag
     1561 tgagcgatgt tctgcgcgca ttaaccgtca gccccgatca agtgctgcaa ctgacgccag
     1621 aaaaaatcgc gatgctgccg caaggcagtc gctgcaacag ctggcggttg ggtactgacg
     1681 aaccgctatc actggaaggc gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa
     1741 acccaacggc acgcgccgcg ttatggcaac agatttgcac atatgaacac gatttcttcc
     1801 ctcgaaacga ctgatttacc agcggcttac cacattgaac aacgcgccca cgcctttccg
     1861 tggagtgaaa aaaactttgc cagcaaccag ggcgagcgtt atctcaactt tcagttaacg
     1921 caaaacggca aaatggcggc gtttgctatt acgcaagtgg tgctggatga agctacattg
     1981 ttcaatattg tggtcgatcc tgactatcag cgtcagggat tgggaagggt gctgctgaaa
     2041 catctgatcg acgaactgga aaaacgcggc gtggcgacac tatggctgga agtccgtgct
     2101 tcaaacgctg ccgccattgc cctgtacgaa agtttaggct ttaacgaggc gacgattcgc
     2161 cgcaattact accccaccac ggacggtcgc gaagacgcca tcatcatggc gttgccaatc
     2221 agtatgtaag acaaggtgga ataatgaagt gggactggat tttctttgat gccgatgaaa
     2281 cgctgtttac ctttgactca ttcaccggcc tgcagcggat gtttcttgat tacagcgtca
     2341 cctttaccgc tgaagatttt caggactatc aggccgttaa caagccactg tgggtggatt
     2401 atcaaaacgg cgcgatcact tcattacagc ttcagcacgg gcgtttcgag agctgggccg
     2461 aacggctgaa agttgaagca ggcttgctta acgatgcctt tattaatgcg atggcggaaa
     2521 tctgcacgcc gctgccgggc gcggtttctc tgcttaacgc cattcgtggc aacgctaaaa
     2581 tcggcatcat caccaacggt tttagcgcct tgcagcaagt gcgtctggaa cggacgggcc
     2641 tgcgtgatta ctttgatttg ctggtgattt ccgaagaagt tggcattgcc aaaccgaata
     2701 agaaaatttt cgattatgcg ctggaactgg cgggcaatcc tgaccgttca cgcgtgctga
     2761 tggttggcga cactgccgag tccgatattc tcggtggcat caacgccggg cttgcgactt
     2821 gctggctgaa tgcgcaccat ctcgagcaac cagaaggcat cgcgcccacc tggaccgttt
     2881 catcgttgca cgaactggag cagctcctgt gtaaacactg attgcctccc ccccgttgat
     2941 gggtaaaata gccgcaattt ttcgttttca acaagcgcgg cgcgatgccg cttactcaag
     3001 aagaaagaat tatgacgttg tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg
     3061 ccattatttc tcacccggac gccggtaaaa ctaccatcac cgagaaggtg ctgctgttcg
     3121 gacaggccat tcagaccgct ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt
     3181 cggactggat ggagatggaa aagcagcgtg ggatctccat tactacgtct gtgatgcagt
     3241 ttccgtatca cgattgcctg gttaacctgc tagacacccc ggggcacgaa gacttctcgg
     3301 aagataccta tcgtaccctg acagcggtgg actgctgcct gatggttatc gacgctgcaa
     3361 aaggtgttga agatcgtacc cgtaagctga tggaagttac ccgtctgcgc gacacgccga
     3421 tcctcacctt tatgaacaaa cttgaccgtg atatccgcga cccgatggaa ctgctcgatg
     3481 aagttgagaa cgagctgaaa attggctgcg caccgatcac ctggccgatt ggctgcggca
     3541 agctgtttaa aggcgtttac cacctttata aagacgaaac ctatctttat cagagcggta
     3601 aaggccacac gattcaggaa gtccgtattg ttaaagggct gaataacccg gatctcgacg
     3661 ctgcggttgg tgaagatctg gcacagcagc tacgtgacga actggaactg gtgaaaggcg
     3721 cgtctaacga gttcgacaaa gaactgttcc ttgcgggcga aatcactccg gtattcttcg
     3781 gtactgcgct gggtaacttc ggcgttgatc atatgctgga tggcctggtg gagtgggccc
     3841 ctgcgccgat gccgcgtcag actgataccc gtaccgtaga ggcgagcgaa gacaaattta
     3901 ccggcttcgt atttaaaatt caggccaata tggacccgaa acatcgcgac cgcgtggcgt
     3961 ttatgcgcgt agtatccggt aaatatgaaa aaggcatgaa gctgcgccag gtgcgtactg
     4021 cgaaagatgt ggttatctcc gacgcgctga cctttatggc gggcgaccgt tcgcacgttg
     4081 aagaagcgta tccgggcgat atcctcggcc tgcacaacca cggcactatt cagatcggcg
     4141 acacctttac ccagggtgag atgatgaagt tcaccggtat tccgaacttc gcaccagaac
     4201 tgttccgtcg tatccgcctg aaagatccgc tgaagcaaaa acagctgctc aaagggctgg
     4261 tacagctttc cgaagagggc gcggtgcagg tgttccgtcc gatctccaac aacgatttga
     4321 tcgttggtgc tgttggtgtg ctgcagtttg atgtggtggt atcgcgcctg aagagcgaat
     4381 acaacgttga agcagtatat gaatcagtca acgttgccac tgcccgctgg gtagaatgtg
     4441 cggacgcgaa gaaattcgaa gagttcaagc gtaagaacga aagccaactg gcgcttgatg
     4501 gcggcgataa cctcgcttac atcgctacca gcatggtcaa cctgcgcctg gcgcaggaac
     4561 gttatcccga cgttcagttc caccagaccc gcgagcatta attcttgcct ccagggcgcg
     4621 gtagccgctg cgccctgtca atttcccttc cttattagcc gcttacggaa tgttcttaaa
     4681 acattcactt ttgcttatgt tttcgctgat atcacgagcg gtttcaaaat tgtgatctat
     4741 atttaacaaa gtaatgacat ttctgacggc gttaaatacc gttcaatgcg tagatatcag
     4801 tatctaaagc cgtcgattgt cattctaccg atattaataa ctgattcaga ggctgtaatg
     4861 gtcgttattc atcacccatc gcttttgtga tggcggccat tgacttctgt agagggtgaa
     4921 gtctctccct attcagcaat gcaacctcgt gttgccaggc tcaaattacg agcaaacata
     4981 caggaataaa tcgatgacta tgacaagact gaagatttcg aaaactctgc tggctgtaat
     5041 gttgacctct gccgtcgcga ccggctctgc ctacgcggaa aacaacgcac agactaccaa
     5101 tgaaagcgca gggcaaaaag tcgatagctc tatgaataaa gtcggtaatt tcatggatga
     5161 cagcgccatc accgcgaaag tgaaggcggc gctggtggat catgacaaca tcaagagcac
     5221 cgatatctct gtcaaaaccg agcaaaaagt cgtgaccctg agcggtttcg ttgaaagcta
     5281 ggctcaggcc gaagaggcag tgaaagtggc gaaaggcgtt gaaggggtga cctctgtcag
     5341 cgacaaactg cacgttcgcg acgctaaaga aggctcggtg aagggctacg cgggtgacac
     5401 cgccaccacc agtgaaatca aagccaaact gctggcggac gatatcgtcc cttcccgtca
     5461 tgtgaaagtt gaaaccaccg acggcgtggt tcagctctcc ggtaccgtag attctcaggc
     5521 acaaagtgac cgtgctgaaa gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa
     5581 tgatctgaaa actaagtaat tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg
     5641 atgtgcacca cactaaaaat atcgcattga gtgattttca cgctcatatc aagcggtgac
     5701 attaactatg gtaaaggaga cgcttatgtt tcgttggggc atcatatttc tggttatcgc
     5761 gttaatcgcc gccgcacttg ggtttggtgg tttggccggt accgctgcag gcgcagctaa
     5821 aattgtcttt gtcgtcggga ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg
     5881 accctagatt tcagaccatt aagaaaattt tatccaaagc cagtccagcg gactggcttt
     5941 tgcggtttta gcgaataata aattgcgtta ctttgtcatt ctttcacaac aaaaacagga
     6001 aggcagaggt ggggcagcga atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa
     6061 ggccgttcca gcctggacga atagctctgg tgtgcgaagg cggcggacag cgtggaattt
     6121 tcacggctgg cgtgctggat gagtttatgc gcgcgcagtt taatcctttc gatctttatc
     6181 tcggcacatc tgccggggcg cagaacctct cggcgtttat ctgcaatcag cccggttacg
     6241 cgcgtaaagt catcatgcgc tataccacaa aacgcgaatt tttcgatcca ttgcgctttg
     6301 tccgtggagg aaatcttatc gatctcgact ggctggtgga ggccaccgca agccagatgc
     6361 cgttgcaaat ggataccgcc gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg
     6421 cctgtcgtca ggatgactac gcgcctaatt actttttacc aaccaaacaa aactggctgg
     6481 atgtgattcg cgcctccagt gcgatacctg gcttttatcg tagcggagtg tcgctggaag
     6541 gcattaacta cctggatggc gggatcagtg atgcgattcc ggttaaagag gcggcaaggc
     6601 agggcgctaa aacgttggtc gtcattcgca ctgtgccgtc acaaatgtac tacacgccgc
     6661 agtggttcaa acgcatggaa cgctggctgg gtgacagtag cctgcagccg ctggtcaatc
     6721 tggtgcagca tcatgaaacc agctatcgtg acattcagca atttattgag aaaccaccgg
     6781 gcaagctgcg gatattcgaa atttatccgc cgaagccatt acatagtatc gcgcttggca
     6841 gtcggattcc ggcgctgcgt gaagactata aacttgggcg tttatgcggt tgttatttcc
     6901 tcgccacggt tggcaagcta ttaactgaaa aagcgccgct tacccgccat ctggtgccag
     6961 tggtgacgcc ggaatcgatc gttattccgc ctgcgccagt cgccaacgat acgctggttg
     7021 ccgaagtgat tgacgctccg caggcgaacg acccgacatt taacaatgag gatctggctt
     7081 gatttgccgt tttatcgaca cccactgcca ttttgatttc ctgccgttta gtggcgatga
     7141 agaggccagc ctgcaacgcg cggcacaagc gggcgtaggc aagatcattg ttccggcgac
     7201 agaggcggaa aattttgccc gtgtgcaggc gttagcggaa aaatatcaac cgctgtatgc
     7261 cgcattgggc ttgcatccag gtatgttgga aaaacatagc gatgtgtctc ttgagcagct
     7321 acagcaggcg ctggaaaggc gtccggcgaa ggtggtggca gtgggggagg tcggtctgga
     7381 tctcttcggc gacgatccgc aacttgagag gcagcagtgg ttactcgacg aacagctgaa
     7441 actggcgaaa cgctacgatc tgccggtgat cctgcattca cggcgcacac acgacaaact
     7501 ggcgatgcat cttaaacgcc acgatttatc gcgcactggc gtggttcacg gtttttccgg
     7561 cagcctgcaa caggccgagc ggtttgtaca gctgggctac aaaatcggcg taggcggtac
     7621 tatcacctat ccacgcgcca gtaaaacccg cgatgtcatc gcaaaattac cgctggcatc
     7681 gttgttgctg gaaaccgacg cgccggatat gccgctcaac ggctttcagg ggcagcctaa
     7741 ccgaccggaa caggctgtgc gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga
     7801 agatgagatt gccgaagtgt tgcttaataa tacgtatgcg ttgtttagcg ttagtgggta
     7861 gtttaccctt cgtaggcagt atccggcagt cggcgcataa tgcctgatgc gacgcttgtc
     7921 gcgtcttatc atgcctacag gtttgtgccg aaccgtaggc cggataaggc gttcacgccg
     7981 catccggcag ttggcgcaca atgcctgatg cgacgcttgt cgcgtcttat catgcctaca
     8041 agtctgtgcc gaaccgtagg ccgtatccgg catgtcacaa atagagcgcc ggaaatatca
     8101 accggctcac cccgcgcacc tttaacgcat cagccaacgg ctcaacgtct tccggcgtgg
     8161 cgctcgccca gctttgcgcc tcgccataca cgccgtgggc atgaaacgca ttcaggcgaa
     8221 ccggaacatc gccaagtctc ttgataaacg tcgccagctc atcgatgtga tgcaaataat
     8281 ccacctggtc tggaattacc agcaaacgca gttccgccag cttgccgcgc tctgcaagca
     8341 aacagatgct gcgcttaatc tgctgattat cgcgtccggt taagtgttga tgacattcgc
     8401 tcccccacgc tttgagatcg agcattgcgc cgtcacacac cgggagtaat ttttcccagc
     8461 cggtttcgct caacatgccg ttactgtcca ccaggcaggt gaggtggcgc agttgcggat
     8521 cgtttttgat agcagtaaac agcgccacta caaacggcag ttgggtcgtg gcttcgccgc
     8581 cgcttaccgt tatcccttca ataaacagca ccgctttgcg gacatggcta agcacttcgt
     8641 ccacgctcat ggattgcgcc attggcgtgg catgttgcgg acacatcttc aggcaggtat
     8701 cacactgctc gcagaccaca gcgttccaca gcactttgcc gtcaacaatc tgcaacgcct
     8761 gatgcggaca ctgtggcacg cactctccgc aatcattgca gcgtcccatc gtccacggat
     8821 tgtgacagtt tttgcagcgc agattgcagc cctgcagaaa cagagccaga cgactgcctg
     8881 gcccgtcaac gcaggagaag gggataatct tactgactaa agcgcatctg ctgttcatgg
     8941 cttatcacgc gcggctggcg ttccagaata cgagtgttgc gtgtggcttc ttcgcccagc
     9001 caggtggtgt tggtgcgtga accttcggcg cgatattttt ctaaatccga caaacgcacc
     9061 atataaccgg taacgcgaac cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt
     9121 ccggctttaa aggcaccgag gcaaagctgt accagcgcct gcggattacg tttgatggtt
     9181 tcgtcgagcg tcagaatgtc gctgatgccg gaataataat aagcatgatg cggggcgaca
     9241 gtttgcagat ggatgatcgg atctggctca tcgccatacg gcaaacgcgc gcccggcgtg
     9301 gtgccgatat cggaactgat ccccgactgt gcgtgtaaca tggcgcgttt ttgccagcca
     9361 tatttcacgg gggtattggc gacaaactcc gccagttgcg cgctgatgcg ataacccact
     9421 tcatttgcgg cggcttcttt accgtagcgc gcggcaatcc cttctttctc acacagcaag
     9481 ttaaccgctt ccgccagccc atacatgcca aacattggca caaaacgttc agggttaatc
     9541 agcccttctt tcaccaggaa gctattctca aagaagtgtg attgttgata gaggaattca
     9601 caccgcgcat cgatgatggc gatctgctgc tggcagtagt gcggtagagt gcgcgtaaag
     9661 aagtcatcca gcgattcgct gcgctcggca atggctttca ggttaaggcg taccagcgtg
     9721 ctgccaccac ccgccagcgg cagtgagttg taacagctca caatcccgta gccccctttt
     9781 gtgaaaattt tatcatgcac cggaccgttg gcgatgtgcg gtttgctaca ttcacagatg
     9841 ttcttcgcca cttccagcag caggtcatca ggggggattt caggatcgta gataaaggtc
     9901 aggttcggtg aaacctgctt caactctgcg tctgcacgta agatcgcgcg ggtaatgggc
     9961 gaatcagacg ggccgatatt ggcgtgcata aaggcgtctg gcagggttcc gtcgaggtaa
    10021 cgccagaaac gttttattcg aacatcgatc tcgtcttgtg ttagaattct aacatgcggt
    10081 tgcaacaacg catccagttg ccctaggtag accggcatcg atgtgaccga cggtacgtgg
    10141 tggtaaagaa tggtcagcag agagagtgcg tcatcaagat ctttcgcgcc ttccagctcc
    10201 agccatt
//
LOCUS       DAJVTY010000146        10177 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVTY010000146 DAJVTY010000000
VERSION     DAJVTY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.146"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="OQQ66_003280"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="OQQ66_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1906094.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            636..2249
                     /gene="trg"
                     /locus_tag="OQQ66_003281"
                     /pseudo
     CDS             636..2249
                     /gene="trg"
                     /locus_tag="OQQ66_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2287..3209)
                     /locus_tag="OQQ66_003282"
                     /pseudo
     CDS             complement(2287..3209)
                     /locus_tag="OQQ66_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3426..4769
                     /locus_tag="OQQ66_003283"
     CDS             3426..4769
                     /locus_tag="OQQ66_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1906095.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4994..6649
                     /gene="opgD"
                     /locus_tag="OQQ66_003284"
                     /gene_synonym="mdoD"
     CDS             4994..6649
                     /gene="opgD"
                     /locus_tag="OQQ66_003284"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1906096.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6789..7013
                     /locus_tag="OQQ66_003285"
     CDS             6789..7013
                     /locus_tag="OQQ66_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1906097.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7076..7612
                     /gene="rimL"
                     /locus_tag="OQQ66_003286"
     CDS             7076..7612
                     /gene="rimL"
                     /locus_tag="OQQ66_003286"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1906098.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7607..8587)
                     /locus_tag="OQQ66_003287"
                     /pseudo
     CDS             complement(7607..8587)
                     /locus_tag="OQQ66_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8711..9703
                     /gene="tehA"
                     /locus_tag="OQQ66_003288"
     CDS             8711..9703
                     /gene="tehA"
                     /locus_tag="OQQ66_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1906099.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9700..>10177
                     /gene="tehB"
                     /locus_tag="OQQ66_003289"
     CDS             9700..>10177
                     /gene="tehB"
                     /locus_tag="OQQ66_003289"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1906100.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRY"
BASE COUNT         2588 a         2388 c         2603 g         2598 t
ORIGIN      
        1 aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgtcagaatc gtaatgcaga taatgagcag acacaccacc agagggttgt gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaagatctg aattgctagg ttcattcgtt
      301 ggcctcggtt gatagaaata tcggtcgggg ccttcgtctt tctgattccc ggttagcctg
      361 aaaacagaaa gtctcaggca cccgcaggca tcctatgagg tttccttagg gacgaaaata
      421 atcacttcac gaaattgcgt gctgttttcc agaatttttc gtcattcgga ttagccagtt
      481 tagccattcg ttactctctt cattccaata gcattaattt tctctgcaat aattgttgta
      541 aaaatgtgac gcaaagaggt ttttggtcat aagtaattac cgtcaagtgc cgatgacttt
      601 ctatcaggag taaacctgga cgagagacaa cggtaatgaa tacaactccc tcacagcgat
      661 taggtttttt gcatcacatc aggctggttc cgttatttgc ctgcattctt ggcggtatct
      721 tagttctatt cgcattaagt tctgctctgg caggccgatc gcgatcagcg tgatgttact
      781 gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg
      841 atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcga
      901 aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg tgcttatcag
      961 aatcgaccgg tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa tcaacgcttt
     1021 caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa
     1081 gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt
     1141 ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga actggcccat
     1201 cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcgtgc cctggtcatg
     1261 acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca
     1321 caacggattg aaaaaatcgc cagtggcgat ctgacgatga atgatgaatc ggcgggtcgt
     1381 aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact ggggatgaca
     1441 gtagggactg ttcgacaggg cgcggaagag atttatcgtg gcaccagcga aatttcagct
     1501 ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga acaaactgcc
     1561 gccagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc
     1621 agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggagtaaaaa
     1681 cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc gtcatcaaca
     1741 gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg
     1801 gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt acggacactc gcaagtcgca
     1861 gcgctcaggc ggcgaaagag attgaaggct tgatcagtga atcagtcagg ttaattgacc
     1921 tggggtcgga tgaggtggca acggccggga aaaccatgag cactattgtt gatgccgtcg
     1981 cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa agtagaggca
     2041 taacgcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag aatgcttctc
     2101 tggtagaaga ggcctcagcg gcggcggtgt cccttgaaga acaggcggca cgattaactg
     2161 aggcggtgga tgtattccgt ctgaacaaac attctgtgtc ggcagaacct cgcggagcgg
     2221 gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga cagatccctt
     2281 cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc aaccagaaga
     2341 gaacatcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat gtcccggaac
     2401 aggctgggcc accagcgtgg catgacgcag gtcgtctttt accgcgccag aagggacaaa
     2461 ccacacgtag tcgtattcaa ccgtaagttg acgagatagc gaagcagaca gcgtttcgat
     2521 acaacccgaa ggaattttac atccctggct ctgtactaat gcatctgaat gctggcgtgg
     2581 cgcagtgcct tctggtgata caacgaccgg ccatttcagc actcggctta gcgttacgtt
     2641 ctcctgaagt agcgggtgat tagggcggac aaccagcttc agcgattcaa gaaacagcag
     2701 ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc cgatatcgat
     2761 ttccccggtt ttcaaacccg ccagaatcat agggttactc attgtcgcaa cttgcaaggt
     2821 cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc
     2881 agtaggtagt gcaccaatcc tgacgacatc attattaaga ccttctttac gatgaagcga
     2941 ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc
     3001 gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc ccgtcagctg
     3061 ctacagttca ttcaatgtct tagagagcgc aggttgactc aaattaaggg tttcagccgc
     3121 gcccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg
     3181 cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg
     3241 acaagtgaag ttgtttgatt atgataactt gattgcaaaa tataattaac aattaaagca
     3301 attgtgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa
     3361 ttcttcccgc cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag
     3421 acactatggc gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt
     3481 cagccatgta ccagcaagaa gttccgcagt acggcacgct gctggaactg gtagctgatg
     3541 tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagatg
     3601 agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc
     3661 ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc
     3721 tctcgcaggc aggggggccg gtacattcga cagcatttcg gcccattgat gatgcttctc
     3781 tggcgcgtaa tcccttccgc gtttttacct ccttactccg ccttgagctt atcgagaacg
     3841 aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt
     3901 gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg
     3961 aatttgtgca agaggccctg gaaacgtttc gctggcacca gtcagcaacg gtagatgaag
     4021 aaacctatcg cgccttgcac aacgaatatc ggttgattgc tgatgtggtc tgttttcctg
     4081 gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga
     4141 tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac
     4201 cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc
     4261 agaaacaggg cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa
     4321 cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata
     4381 atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt
     4441 taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc
     4501 atcgtcaggc gattcatctt ggagatgatc cacagccttt aattgaacgt ggttgggtag
     4561 tggcgcaacc catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt
     4621 caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg
     4681 agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac
     4741 gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc
     4801 ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca
     4861 tattctctca gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa
     4921 ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga
     4981 ctcactttca ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt
     5041 gtgcggtacc agcggcattg cttctctttt ttctcaggcg gcattcgcgg cagattctga
     5101 tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga
     5161 cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat
     5221 gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt
     5281 tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt
     5341 tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga
     5401 gttgttcaaa tacaacgatg cgggtgttga tacaaaacaa ttagaagggc aaagcgatct
     5461 cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc
     5521 atttctcggc gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc
     5581 ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc
     5641 cttctggttt gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga
     5701 tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat
     5761 tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc
     5821 gatgaccagt atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc
     5881 gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg
     5941 tccgctgaat aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg
     6001 gtttggttta ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta
     6061 taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg
     6121 cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgtt tctggcagcc
     6181 agaaaaagct gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc
     6241 gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg
     6301 tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc
     6361 cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat
     6421 tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg
     6481 ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg
     6541 gatgtatcta cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc
     6601 gccagcgccg gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct
     6661 tcggcacctt cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct
     6721 aagttaaatg agtaatctga tggtgtgtat ttcagataca ccttgtcagc cactaacagg
     6781 gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc
     6841 gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag
     6901 gtttcgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt
     6961 taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaaggggtg taaactttcc
     7021 taagccgcca gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac
     7081 tgaaacgata aaagtaagcg aatcacttga attacatgct gttgcggaaa gtcacgtcac
     7141 acctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc
     7201 gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca
     7261 tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat
     7321 ctcgtttaat cgtattgaac cactgaataa aactgctgaa atcggctact ggctggacga
     7381 atctcatcag gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc
     7441 tcagtctggt gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa
     7501 ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct
     7561 gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg
     7621 ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa
     7681 ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa
     7741 ttatcaccgc acctgaaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa
     7801 tcggttcacc acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat
     7861 taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtc ggaatggcat
     7921 cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc
     7981 agacgttgtt ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt
     8041 caataaaagc ataccggacg acggcatcgc cataaatttg tgcctggtgg acaatacgcg
     8101 aggcgctaac ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc
     8161 cttgtgcggc gacgatcata gaatgttgat caattaaggc gtgaccaaat attctacatt
     8221 gcccgtaaac taatgagtca tgaatggtga cactgtcact tatatatgca ccctgactga
     8281 tttcactatt atcgatccag acgttatccg ttgcgtaaac ctctccccac agcacgctgg
     8341 tgccggtgat acgcgtatta ccagaaatca ccgttccgcc gaacgcaata gcattctggt
     8401 cgtaaatcca gcaatttcct tcctatgcta acaccgtttc gcgatcgatc cagccgccag
     8461 cggttcctgc tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacac
     8521 tttttttagt gccatcttcc tgataactaa aggctcgctg ttcttcactt agacgatatt
     8581 tgcgcatagc gttttctcac aggtgactta ctatgaccgt agcaaattct gcggctctgg
     8641 ctatgctcga gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat
     8701 gagtaagaag atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt
     8761 gttggggacg atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag
     8821 ccactggtta ggggatgggc tggtgattct ggcgatgatc atctggggat tattaactag
     8881 cgcatttatt acccgactca tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc
     8941 agtgctgagc agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt
     9001 tgttccgtgg tttcgcccac tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt
     9061 ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac
     9121 catgcctgga ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg
     9181 tgcgttgggc tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct
     9241 aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact
     9301 gcggacatca ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg cctggttgag
     9361 cgtcaacggc ggcgagggtg atacgctggc gaaaatgctt tttggttatg gactgctgca
     9421 actgctgttt atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt
     9481 ctggagtttc tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg
     9541 cagcgaaaat ggatttttcc atacgctggc ggtaccgctg tttatcttta ccaattttat
     9601 tattgcaata ctgctcatcc gtacttttgc gcttctgatg cagggaaaat tgttagtcag
     9661 aaccgagcgc gctgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac
     9721 tattttactg ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa
     9781 gtggttaaac cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac
     9841 ctggcagcca atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaat
     9901 gtagagcgca ttaaatccat tgaaaatctg gataatttac acacccgagt cgttgatctg
     9961 aataacctca catttgatag acagtacgat tttattcttt cgactgtggt actgatgttc
    10021 cttgaggcta aaaccatccc agggctgatt gccaatatgc aacgttgcac taaacttggt
    10081 ggttacaacc tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc
    10141 ccgtttgcct tcaaagaggg agaattacgt cgatatt
//
LOCUS       DAJVTY010000147        10169 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVTY010000147 DAJVTY010000000
VERSION     DAJVTY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.147"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(498..860)
                     /locus_tag="OQQ66_003290"
     CDS             complement(498..860)
                     /locus_tag="OQQ66_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1906101.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1145..1354
                     /locus_tag="OQQ66_003291"
     CDS             1145..1354
                     /locus_tag="OQQ66_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906102.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1366..1953)
                     /gene="mtlR"
                     /locus_tag="OQQ66_003292"
     CDS             complement(1366..1953)
                     /gene="mtlR"
                     /locus_tag="OQQ66_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1906103.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1953..3101)
                     /gene="mtlD"
                     /locus_tag="OQQ66_003293"
     CDS             complement(1953..3101)
                     /gene="mtlD"
                     /locus_tag="OQQ66_003293"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1906104.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3331..5232)
                     /gene="mtlA"
                     /locus_tag="OQQ66_003294"
     CDS             complement(3331..5232)
                     /gene="mtlA"
                     /locus_tag="OQQ66_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1906105.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            5769..6131
                     /locus_tag="OQQ66_003295"
     CDS             5769..6131
                     /locus_tag="OQQ66_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1906106.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6134..7270
                     /locus_tag="OQQ66_003296"
     CDS             6134..7270
                     /locus_tag="OQQ66_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1906107.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(7730..8167)
                     /locus_tag="OQQ66_003297"
     CDS             complement(7730..8167)
                     /locus_tag="OQQ66_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906108.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(8875..9336)
                     /locus_tag="OQQ66_003298"
     CDS             complement(8875..9336)
                     /locus_tag="OQQ66_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1906109.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(9348..>10169)
                     /locus_tag="OQQ66_003299"
     CDS             complement(9348..>10169)
                     /locus_tag="OQQ66_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1906110.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDE
                     NPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     VINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDL
                     AYLIKFDGNYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNG
                     KLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT         2606 a         2406 c         2316 g         2841 t
ORIGIN      
        1 ttgtccattg tattattggt gatttatgtt gctttacatt aatgacagtg atgtgtgtcg
       61 attaaatatt gttttaagca ttcatcataa aaatgtatca ttcgtttcaa aagatgtctg
      121 tgtttgtatg aatatattat tttcgtggat tctaatgatg attattaata aatgtcaaat
      181 atgaaagaaa ccatgtctgg gaaaaaattt acagataatt tattgtatga atatgatgat
      241 taaaatttca caattaaatc cgaaaaaaag agacgtaact gcttgttaat taataaatga
      301 tatcttctat atatttttat tgtttatagg tggctatttc ggtaaaaatg cattttcaga
      361 atatcaacca gcggtaaatc gacgtaaagg gagtgaattg tttggtaatt aggcgagtag
      421 acaggagagt ccccgcattg accagctaag gcaagccaga tgcgggttga tatgatcgaa
      481 ccagtcgtct gaggcgctta aaacgcagtc ttagagaatc cagtcatctc ctgcaggccc
      541 atttcacggc ccagcgcggt cattgggtgc acaaccacca gtccgcgaac acttttcttc
      601 agcttgccca tatcagcctg ctcttttttg gtaattgcgc gctgaaacgg catcttcatc
      661 agcttctgtg cttctttact cagtttttgg ctatgaacct cacgcaggcg agcaatttct
      721 gcttccagcg tggctttctc tttttccagc tcggcatatt tgtctgcggc ttcaaccagc
      781 gacagatcgg cctgctggtg gcggatggcg tccaggcgat cgctgagacg tttgatttcg
      841 tttttttcga cttctttcat gtgatggctt ctgataaaga taaatttata gcccctagta
      901 tgcgcgacca ggtgctttgt tcacaactga tgacattgta atagttctgc aacgtaatca
      961 ttaacatgct caaaaactac gcaattcgtt gattaattaa gaaatctata gaaaaaagat
     1021 aataaacagc agcggatgat tattgaggat tcagcacgtt ttccttaatg tcatctttac
     1081 cgggaaccta aactaaaatt ggtggccgcg cattcccggc gcggggtaat acggagatat
     1141 catcatgggc aaattaggtg aaaacgttcc gcttcttatc gataaagccg tagatttcat
     1201 ggcatcaagc caggcgttcc gggagtatct gaaaaaactt cctccccgta acgcgattcc
     1261 gtccggaata cccgatgaaa gcgtgccgtt atatctacaa cgtctggagt attatcgtcg
     1321 gctttatcgg ccgaagcagg tagaggggca gtaagcagac gttgcttact tttgaaaggc
     1381 tttttttaag ctgattttgg aaatcagctc agtcagtgaa aggaccattg ttgatcgcac
     1441 agcctgttga tagcgctgaa tttgcattgc atacaaactg gagtctgctg gttcaaactg
     1501 tggcggcggt ggtaacgccg ccacgcagtg cagttcacca aagggtccaa ggatttcgtc
     1561 gtcggtaaag gcgtactcgt tgccgtcgtg atttagctct tcacgcaatg ccatcagcag
     1621 ttccgcatct tcgtattcct ggcggttaat gacgcccaac ccgtaaatga gttttaaacg
     1681 cacagaaaga tcgcccagcg gaccgtcgcc gtcgagtaac ggttctacag catacttcac
     1741 cgcgtaatcg tctttgcgga atacctgaag caccagaaga tttaccgcct cggtcaaaag
     1801 ctcgacggcg gtgatcagaa agcttcgcac ggttttgcca gcattcagac gctcaagcac
     1861 acggttttca aaggcctggg tttgttccat tgttgcctgc atatctgaca atctgttatt
     1921 cgggcgcagg gtgtcctgcg cctggtccac cattattgca ttgctttata agcggttacc
     1981 gcctcggata caacctcact gttggcatca agaccggaaa tctgtgccag cgccgcctgc
     2041 ggacctttgt cagcgatcag tgctgccagt tcctgagcct gcggatcatc ttcactgcgg
     2101 aagtgcattg caccagcaat cccctgaatc agattcttgt gcggaaggct gtattccagc
     2161 gtaccgagca gtggcttgat cagacggtcg ccagcactca gtttacgcag cggctgacgg
     2221 cctacgcgct ctacatcatc tttcagatac gggttctcaa aacgaccgag gattttctgg
     2281 atgtacgccg catgtttgtc tgcgtcaaag ccgtagcgct tgatcagtac cgcaccactt
     2341 tcttccatcg caccttttac caccgcgcgg attttctcgt cgagaatagc gtcacgaatg
     2401 gtctgatgac cggccagttt tccgaggtac gcggttatag catgacccgt gttcagggtg
     2461 aagagtttac gttcgacaaa tgccatcagg ttgtcggtta actccatgct tgggatgttc
     2521 ggcagtgcgc ctttgaactg cgttttatcg acaatccatt cgctgaaagt ttctaccgtc
     2581 acttccagcg gatcgttagt tgccgaagcc gaaggcggta cgatgcggtc aacggcggaa
     2641 tcgacaaagc caacgtgttc ttctacccac gctttggcgt cttccggcag ggcgttcatc
     2701 acatggcctt tcagctgcgt ggtaccgcgt accatgtttt cacaggcgat gatgttcagc
     2761 ggggattcat taccttgttc tttgcgtttc accagtcctt tggcgatagc cggagcaata
     2821 cgttccagca caaccgggcc aacggcggta gtgactaaat cgacctgagc aatcagatca
     2881 acgacatcat caccaatgct gctgacagca ttgacgccgg aaacggtatc tacctgctcg
     2941 gtttcaccaa ccacatgaac ctgatagcta tgacgggcat tcagggcatc aagtaccacc
     3001 tgattgacat cggcaaacgt cagttggata cccgcgtctg ccagcagttt accgataaag
     3061 ccacgaccga tattacctgc gccaaaatgt aatgctttca tagtattaac cttcatcaat
     3121 gtttttaccc gagagggctg gggtgaggct tttccctcac ccaaaccctc tccgtgagga
     3181 gaggggcagc gccggatgcg gcgtaagcgc cttatccggc ctacaagaac gtgcaaattc
     3241 aacaaatcgt gatgcatcct gtaggcctga taagcgtagc gcatcaggca attaacccca
     3301 ccttctccat gtggagaggg tgggattgga ttacttacga cctgccaaca gttccagcac
     3361 ttcatccacg ctggtggtgt gtgccagacg ctcgatgacg gactcatcat ccagtgcatt
     3421 ggtcaggctg gtgataacct gaatgtgctc gttgttacgg gctgcaatac caatcaccag
     3481 acgggcaatg tcatcttcct cttcaccgaa gcgcacgcct tccgggtact ggcagaacac
     3541 cacgcccgtt ttcagtacgc gatctttcgc ttcaaccgta ccgtgtggca ccgcgataga
     3601 ctcacccaga taagtcgggg tcagtttttc acgatccagc atcgcctgaa cgtattccgg
     3661 ctcaacgtaa ccgcctttca ccagctgctc gccagcaaaa cgaatcgctt cttctttggt
     3721 tgctgctttg cgaccgagga agatgttctc cgcgcccagt ttgaacaggt tagcactgga
     3781 atcgtcaaag ctgtctttca ctttcacttc gttttcagta tggcgctggg cagcaaccag
     3841 acgttcggtt aggctggtgt acaggccgct gtcgaggaag ttggtcagcg aaatatgctg
     3901 tgcctgcgga acctggcgca tagcgcgttc ggtcaggtca cggtgagtga tgacgaggtc
     3961 cacatctggc ggcaggttgt tgatcgcgct gttagtgacc gaaatctgcg acagacctgc
     4021 atcctgaatt ttcttacgca gcacgcctgc gcccatcgca cttgaaccca taccggcatc
     4081 acaggcaacg atgattttac gtacgtggct caggtcgtta gtcacatcgc ccgcagacag
     4141 cggagatgtg cctttagact cagctttcat gtcctgcata cgacgagttg ctgcttcaat
     4201 atcatcttct tctttcactt tgctggtttt cagcaaaata gcagagacaa cgaaggagac
     4261 agccatcgcc gcacacacac ccgcgatgtt agcgaaataa gccccttttg gtgtcatcgc
     4321 cagtacagca aggatagaac ccggagatgc cggagaaacc agaccaccgc ccaggatagt
     4381 cagcgtgaac acgccagtca taccgccgag gatgactgcg aggatcagac gcggattcat
     4441 cagcacatac gggaagtaga tttcgtggat accacccagg aagtggatga ttgccgcacc
     4501 gcccgcagac tgtttagcgc taccacgacc aaagaacatg tacgccagca gcacgcccat
     4561 acctggacct gggttagctt caatcaggaa gaagattgat ttacccagtt catgggactg
     4621 ctgaataccc agcggcgaga agataccgtg gttaatggcg ttgttgagga acaggatttt
     4681 cgccggttca acaaagatag acgccagcgg cagcatgtca tggacaacca tgaagttaac
     4741 gcccgcagcc agcattttgg acagggcttc aacaatcggg ccaatgccga ggaatgccag
     4801 aatagcgagg atcatcccga tgatgcctgc ggagaagtta ttcaccagca tctcaaaacc
     4861 ggatttgatc ttaccgtcta cccagcggtc gaagtgctta atgcaccagc cgcccagcgg
     4921 acctgcaatc atagaaccga ggaacatcgg catgtctgcg ccgacgataa cgcccatggt
     4981 ggtgatggca ccgactacgc cgccacgttc gccgcctacc agcttaccac cggtataacc
     5041 gatcagcagc ggcaggagat aagtgatcat cggcccgacc agcttcgcca gcgtctcgtt
     5101 cgttaaccac cctgttggaa taaataacgc ggtgatgata ccccacgcga taaacgcgcc
     5161 gatatttggc atcaccatgt tgctgaggaa acgaccaaag ctttgcactt tgatcttaat
     5221 atcggatgac ataaaaacac cccttcttat gtttgctgtc gcgcaggctg gaagcccgag
     5281 gttttttgtt aatgtggcgg cagaggtagc cggaccttgt agatactgcg aaatctggca
     5341 ctgaatcggt taactgtcca gtcgacggcc ttttgtgtga tattaatcac ataaatatag
     5401 gaggtagaga ggtaattgac gtgacgttca tcacaaaacg cttgtgtaaa aaagcaacaa
     5461 ggcggcgatt ttcgctaaaa atggcattta aatgtgagta gtatcacatt tttgttttga
     5521 tggtttgttg aatctttgtg atctgaatca caagatattt tcttacggcg ttcacctgat
     5581 gtgatctaca gcatgttatg ccttcgtcat acaatcgtta tgtaactaag caactcatca
     5641 cctctaaaaa ttattctttg gtgttatggc gagaatcgcg aacccggatt attgtcaaaa
     5701 tatcagcctg actcccttct ggctgccagc ccaaaaacaa cactgataaa cgtgaatccg
     5761 aggcagatat gtttctaaac tatttcgcgt tgggagtgct gatcttcgtc tttctggtga
     5821 ttttttatgg aatcatcgcg atacatgaca tcccttatct gattgccaaa aagcgcaatc
     5881 acccccatgc cgacgctatt catacggcgg gctgggtgag cctgtttact ctgcatgtta
     5941 tctggccgtt tctgtggatc tgggcgacgc tctatcaacc ggagcgtggc tggggtatgc
     6001 agtcacatgt tgcgtcgcag gagaaagcga ctgacccgga aatcgcctca ctttctgacc
     6061 gaatttcccg gctggagcat caactcgccg ccgagaaaaa gactgactat tccacgttcc
     6121 cggagatcta attatggatc tattgattgt tttaacttac gtggcgctgg cgtgggcggt
     6181 ctttaaaatc ttccgcattc cggtaaatca gtggacgctg gcgacggcgg cgctgggagg
     6241 cgtgtttctg gtgagtggtt tgattttgtt gatgaactac aaccaccctt acacttttac
     6301 cgcgcaaaag gcagtgatag cgatccctat cacgccacag gtgacgggaa ttgttactga
     6361 agtcactgac aagaataatc agcttattca aaagggcgag gtgcttttta agctcgaccc
     6421 ggttcgttac caggcgcgag ttgacagact tcaggctgac ctgatgacgg cgacgcataa
     6481 tataaagacg ctgcgtgcgc agctcactga agcgcaggcc aacaccaccc aggtttcagc
     6541 ggagcgcgac cgtctgttta aaaattatca acgttacttg aaaggcagcc aggcggcggt
     6601 gaatccgttc tcggaacgtg acatcgacga tgcgcggcaa aatttcctcg cgcaggatgc
     6661 gctggtgaaa ggctcggtgg cggagcaggc gcagatccag agccagctcg acagtatggt
     6721 taacggcgag caatcgcaga ttgtgagctt aagagcgcaa cttactgaag caaaatataa
     6781 ccttgagcag actgtcattc gcgcgccgag caatggctac gttactcagg tactgatccg
     6841 cccaggtaca tacgcagctg ccttgccgct gcgtccggtg atggtcttca tccccgagca
     6901 aaaacggcaa attgtcgccc aatttcggca aaactcgctg ttacgtctga aacccggcga
     6961 tgatgcggaa gtggtgttta acgcgctacc tgggcaggtg tttcacggca aactgactag
     7021 tattttacct gtcgtgccag gcggttctta tcaggcgcag gggggattgc aatcattaac
     7081 ggtcgtgccc ggcacggacg gtgtgcaggg aaccattgaa ctggacccta acgatgatat
     7141 cgatgcctta cccgacggca tctacgccca ggtggcggtt tactccgacc atttcagcca
     7201 tgtttcggtg atgcggaaag tgctgctaag aatgaccagc tggatgcatt atctttattt
     7261 ggatcattga gggggagggg ggagattatg gaactttgct ccttgtaagg actaattatt
     7321 tttgcatatt acttcattag ctgcgatgat ttttctttaa tattcattat gttaataagt
     7381 tctggatgag tgtgctctca ttggattttt tatccgtctt gttctgccgc aggatgagac
     7441 tatacatcgt cgagatcgca atgatgatat gagttgttcc tcctgcgacg gctctaatca
     7501 aaaaagagca ggagtgcttt tccatctatt gcgatgatgg ctttattatc tgaaggatga
     7561 cagtcacatt taaaaaatat ttaatctata gatcacaatg tagggtcatg gaatatatgt
     7621 atacaactat tttcttcttc acttttaatt atcgtattca ttataaaact aagttaaaaa
     7681 aactcctgga gagtgagtta ttaatatatt tttaaataaa attaagttat cattcatcga
     7741 tgcattttat agaatcatca atggccataa tagtactatt aactctcaat tttatatttt
     7801 tatcatcact acaaatatta actgaattta tatcaatgat ttttttattc ttatctttta
     7861 ttttatttac tgtttcaatg caatcagcgg caagttggag tggatttaga tttttcagat
     7921 atggtcttat gatctttccc ttacttaata aatagcatgt ataggattcg gaaagtccag
     7981 catctatttt gtacctcatt atattagata aggatgtgag aaattccttg ttatcattcg
     8041 cagccatata tgccagtccg agttcagcct ctgcggcctt acaggcaaaa tagcttagtg
     8101 aacacgcttt tcgttcctct ttagtactgg ctgatattag tgcccataat attgctttgt
     8161 ctgccaaatc taactctctc tgaaattgtc ttgcagtaac tgcactcgca gaaatagaga
     8221 aaaaaataca attgttaata accttgccat atttttttag tccgtaactg ttaatgtgct
     8281 atctggctct gaaaatatta actcatttaa tttttgcatg tcattagata aaccagttat
     8341 acaaccctcg gaacagaatc gaggtcctaa agacggacca aaagcgttta aacaactatg
     8401 tattgaaaat ccattgcgat tataagttcc tgtattaact gacggtacta atcgtatgcc
     8461 gtcaggtttt ctattgcttg ctcccgggtc attaacattc caactccata ggccttgagg
     8521 tattggccat ctatttataa atgtgtacaa cggggattat ttttacattg catattatta
     8581 ccagaagcga ctggaatact aagcacatca taaaaattat catcacatgg tatatctgaa
     8641 acacacgaaa gcatacctaa agttatgctg tataaacagg tggctaatcc caaggggatc
     8701 gattccagaa atcgggttca acggatactg ataaaaattc cacctacctt ccagcccaat
     8761 cggagcctga gtgttatacc gcccctgctg cggttaatgt taccgataat agttatagta
     8821 cagccctgat tcatcatcat actactgctg cggcagtctg atgaactatt gcttttacgg
     8881 attctcctta ttcttccttc tatccattcg ctttagcttt ttgagctctt gaagtttgct
     8941 ctttgcatca atattaccgt tatttacagc gaattccatc caataaatag ctctattcaa
     9001 attataatat tctgataatg ttggatttgt atctgataaa aagaccccat aattaaactg
     9061 cgctgtaaca ttcccctgag atgctgatct ttccaagaat cgcaattgtt tatcaatatt
     9121 atttttagta aagcagtaat attgatatag tcgaaatgat gcctctgaat caccatgttg
     9181 tgatttatac ttcagtttca tcagcttttc ttcacttaaa tcataggtca ttgctgtaga
     9241 tcccatcaca ctttcgtttt cttttatttt cacatcagca ccatgcaatt gatcaataag
     9301 agaaaaataa aaaaatagta acaagcatgc tttcatacat ctacaaccta tcttatatca
     9361 ctgggttttt ctggcgtacc ttgcatatta gaaatggggt gcccagatgg cccatatgtt
     9421 atccaattac caaggtcttt tgttgttata gccattagat ccttatttga attaaggttc
     9481 gttggtgctg ttagcactgt atccattttt aacttaccat tagttcctcg cccccaccac
     9541 gtccatagcg tgtcaggtcc tattacattt gtatgtaact ctttagctaa ttggctagca
     9601 aatgaatttt gtcctttact tgtattacaa gaaaataacc aaactgtcat cccatcttta
     9661 taattcccat caaatttaat tagatatgct agatctttag ctgtcatgat actgcgcgtt
     9721 gcggattcaa tagatgtggg ggtaccatgc cccccgattg tgaaaacgcc tgggatatta
     9781 atctcatcag ctacagaatg gataagatca ctttcggggt ataaatttat atcaaccaaa
     9841 ccttgcggat ctacatttat gaccggattc aacggatact gataaaaatt ccatcccccc
     9901 ttcagcccaa tcggatcctg agtgatatac cgcccctgca gcgggtcata atagcggtgg
     9961 cggttgtaat acaggccgga ctcctcatca tactgctgac ccggcaggcg aatgagctgc
    10021 tgcagatgat gcgggttctc atcactcagc aggttgctcc attcatcata ttctgcgcac
    10081 cacgctgttg ccccttccgt gctgatgagc gccagcggca ggccgcgatg gtcgcagtgg
    10141 tacaggtgga tttttcgcgc cggcgtgta
//
LOCUS       DAJVTY010000148        10121 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVTY010000148 DAJVTY010000000
VERSION     DAJVTY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.148"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..275
                     /locus_tag="OQQ66_003300"
     CDS             <1..275
                     /locus_tag="OQQ66_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1906111.1"
                     /translation="ALVVAAAMGLSSAAFAAETATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            368..451
                     /locus_tag="OQQ66_003301"
     CDS             368..451
                     /locus_tag="OQQ66_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906112.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            551..1372
                     /locus_tag="OQQ66_003302"
     CDS             551..1372
                     /locus_tag="OQQ66_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1906113.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1411..1740)
                     /gene="mdtI"
                     /locus_tag="OQQ66_003303"
     CDS             complement(1411..1740)
                     /gene="mdtI"
                     /locus_tag="OQQ66_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1906114.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1727..2092)
                     /gene="mdtJ"
                     /locus_tag="OQQ66_003304"
     CDS             complement(1727..2092)
                     /gene="mdtJ"
                     /locus_tag="OQQ66_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1906115.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2504..2647
                     /locus_tag="OQQ66_003305"
                     /pseudo
     CDS             2504..2647
                     /locus_tag="OQQ66_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(3172..3453)
                     /locus_tag="OQQ66_003306"
     CDS             complement(3172..3453)
                     /locus_tag="OQQ66_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906116.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(3467..5128)
                     /locus_tag="OQQ66_003307"
     CDS             complement(3467..5128)
                     /locus_tag="OQQ66_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1906117.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(5112..5468)
                     /locus_tag="OQQ66_003308"
     CDS             complement(5112..5468)
                     /locus_tag="OQQ66_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1906118.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(5591..5773)
                     /locus_tag="OQQ66_003309"
     CDS             complement(5591..5773)
                     /locus_tag="OQQ66_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906119.1"
                     /translation="MTLMNEREQKRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(5757..6197)
                     /locus_tag="OQQ66_003310"
     CDS             complement(5757..6197)
                     /locus_tag="OQQ66_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1906120.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWEKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(6197..6493)
                     /locus_tag="OQQ66_003311"
     CDS             complement(6197..6493)
                     /locus_tag="OQQ66_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1906121.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQLVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(6490..6828)
                     /locus_tag="OQQ66_003312"
     CDS             complement(6490..6828)
                     /locus_tag="OQQ66_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1906122.1"
                     /translation="MNIGRLRDRVTIQTLKQTRDITGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(6825..8036)
                     /locus_tag="OQQ66_003313"
     CDS             complement(6825..8036)
                     /locus_tag="OQQ66_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1906123.1"
                     /translation="MWLFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(8038..8610)
                     /locus_tag="OQQ66_003314"
     CDS             complement(8038..8610)
                     /locus_tag="OQQ66_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1906124.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAVGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            complement(8650..9807)
                     /locus_tag="OQQ66_003315"
     CDS             complement(8650..9807)
                     /locus_tag="OQQ66_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1906125.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRSYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGVTVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
BASE COUNT         2336 a         2767 c         2430 g         2588 t
ORIGIN      
        1 tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct gcagagactg
       61 cgacgaccac caaagcagcg ccggcgaaaa cgacacatca taaaaaacag cataaagcag
      121 cacctgccca gaaagcgcag gcagctaaaa agcatcataa aaatacgaaa gctgaacaga
      181 aagcccctga acaaaaagcg caggcagcga agaaacacgc cgggaaacac agtcatcagc
      241 aaccggcaaa acctgctgca caacccgcag cgtaagtttt caacagtaat gctggcgcgc
      301 cccctcgcgc ctgaaaatta cggtgctaag cgggtaacgt ttagcaccgc ctttagcgga
      361 gggtagtatg gtgggccgtt atcgctttga gttcattctt atcatcctta ttttatgcgc
      421 actgattgcc gcccgttttt atctttcctg actgtagtta tctgatttta ctcccacttt
      481 catcccgtcc cgtctatagt atttatgagg gtttgctttt aataatcata attacccacc
      541 agagtgtgat atgcgtacaa ccattgctgt agtgttgggt gcaattagtt tgacgtctgc
      601 ttttgtgttt gcagataaac cagacgttgc caaatcggca aacgatgagg tcagcaccct
      661 gttttttggt catgatgatc gtgtgccagt gaatgacacg gcccaatcac cgtgggatgc
      721 ggttgggcaa ctggaaacgg ccagcggcaa tttatgtacg gcgacgctga ttgcacccaa
      781 tctggcatta acggcaggac actgtttatt gacacctcca aagggtaaag ccgataaagc
      841 agtggtgctg cgttttgtgt caaataaagg tctttggcgc tatgagatcc acgacataga
      901 aggccgcgtt gatccgacac tgggaaagcg gttaaaagca gatggggatg gttggattgt
      961 acctcccgca gccgcgccgt gggacttcgg attgattgtg ctacgtaatc ccccttctgg
     1021 cattacaccg ttgccgttat ttgagggaga taaagccgcg cttactgccg cattaaaatc
     1081 ggctggccgt aaagtaactc aggcgggata ccctgaagat catctcgata cgttgtacag
     1141 tcatcaaaac tgtgaagtga ctggctgggc gcaaacgtcg gtgatgtcac atcagtgcga
     1201 taccttgccg ggtgacagcg gttcgcctct gatgttgcat accaatgacg gctggcaatt
     1261 aattggggtg caaagttcgg ctcctgccgc gaaagatcgc tggcgcgccg ataaccgggc
     1321 catttctgtt accggttttc gcgacaagct ggatcaactg tcgcaaaaat aatgttcaaa
     1381 cgctgcccga ctgtgcgggc agcgtgttca tcaggcaagt ttcaccatga tcattccagc
     1441 caacagcaag accaggccaa tccagccttt acgatttaac cgttgaccaa acaagatcca
     1501 acctgcggct aacgtggcgg caataccaaa cccgccccac aatgcataag cgacagacaa
     1561 gtcgatccct ttaacggcct gagaaagcgc actaaaggca gccagcaccg ccgccaggga
     1621 gagcaagcca aatattttgc gacgaaagcc gtcagaaaat ttcaaaaaga cgttagcaac
     1681 gatttccagc acgattgcca atgccagcca ggcggcgtga acccattcaa actgcgccat
     1741 ggtttacctc cagttcaggt ttacgcgctt tacgggtacc tgattttatc aacacaatcc
     1801 cggcgaccag ggtggttaac ccggcaattt tcatcagcga taaactttcg tcgaataaca
     1861 aaacgctaaa caaggtaata aataaaatac cgataccttc ccacagcgca taagctacgc
     1921 ctaaggcgat ctttttaacg gcgaaagaga gaaatatata cgacagagaa atcatcacca
     1981 gcattaaaat aaagccgcca tttccctcac tgacgctcgc ccatttcatt gacagcgtac
     2041 cggtaatttc tgtagcaata gccaggccta ataaaatcca ataaatatac attgtccttc
     2101 tcctgcaaga gaattatttt aattttcgct taattcagcg aaattaaagt aaagtggtca
     2161 agccccgcgc atagggcaag cttaggcaga agaaaggact aaagcgcgtt gcgccagtgc
     2221 tgctcaccta cgagcaagat agtggatgag gtacaaagat gggtaaatgt tgaaaataag
     2281 gtcctgaaca aattgtccat aatattacaa ttatccgcag tgttgcttct cgtcatcgcg
     2341 gatgataatt gtcctcggta gttgaacacg cctgatttgt atcatagctt aagaattaac
     2401 tcaaaatatt ttcacttctt tacctgagcg gtttgatttt cgttatgatg acagagcgaa
     2461 aaagacatta ttattagcaa aggaagaaaa aacggggaca agcatggcaa agccgatcat
     2521 cacgctcaat ggcctaaaaa tcgtcattat gttgggaatg ctggtcatta ttctctgcgg
     2581 tatccgtttt gccgccgaga tcatcgtgcc gtttattctc gcattattta ttgctgttat
     2641 atataactta tttcacttaa cgcattgatt taattaaata atttgcacca caaaaagtgc
     2701 caaaaatcgc aaaatgacta caccagtgac tacaccgttc ggtgcactgt atgaaacaac
     2761 gtggaacaaa tagacacaag aaatatacag gcagatcatc tttccagggg gaagcgcacc
     2821 aattcatgag gggcgttgct gtcgatatgg ggatctccac ctacctggat ttttttccgg
     2881 ttaaacgtta atcaggctgg tgggctttac ctgttcggta ggagtggtca ttatgaccat
     2941 tcagacaatg accttaatca gaatttttcg ctaaaaaatg acgttagcca cgtcgtccgg
     3001 aaaacacaaa atcccgtcga tttttaatta acggttaaac cgtcttttgt cacggcgtgt
     3061 cactggctgt cactctccgt cacgattggg cgtggtgatt atcaccattc agaatctaaa
     3121 gggtgacgct tccggcctcc tcaaattgag gtttccgaaa atatcaacca gttaccgccg
     3181 caaccgctcc ggcttcttcc agtggtacgt tattttctcc ttctcccgat acatctccac
     3241 gcggcgacgg taggccagca actcaagaac tctggttcgt atgttgcgca tatccacgcc
     3301 attaagttct ataccatcac ggcgcatcac ctcagcaaca acacgcgcat aattttcggc
     3361 tgtcacgctg tccggctgcg tggcctgttc gtcagcctgc tggctgattc cggcaacgcg
     3421 gcggattaat cccgttattt cggcttctgt catgctgctg acctcattac accccgctaa
     3481 attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc tcatgctctg
     3541 cctggaatgt accaaacgcc atcagcgccg ccacgctcgg gtctatcttg ttcggtgatt
     3601 ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg ttactcatcg
     3661 accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca aaaacttcga
     3721 acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc acctcaaaac
     3781 ctgccccctg taattgcgtt ctgagatgcg tggcgttcca cgtatcaaag cccaccagcc
     3841 tgatattaaa tttctccgcg tcctgcatga tgtcatctct gatccggtcg taatcaatgc
     3901 agtcgccagg cgttgtgcgt atccagcctg ctttagccca ctgacgatag acagcgcggt
     3961 ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa accagcataa
     4021 tggttttacc taccggaaag gcatagcaca cgctggaaat atcgcttgtg gatgataaat
     4081 ccagccccgc gtaacactcc tggccgtgta aatcttcctc cgtgaacgtt ccggcacact
     4141 cagcccatgc gccgttaccc atccacgggg tagtcccctg acaccagata ttaaatcgct
     4201 ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg gttgaggcca
     4261 gtttttcacg atccacggaa acatcaatat tagggttcgc ttttatccac atcgccggat
     4321 catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa tcgttgacct
     4381 cttcgccgtc caggatctgg cagcaataat catagtgctg cttacaggct gaaacgacgt
     4441 tactgcctga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc atccccagct
     4501 caagcgcaga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg atggaaaggc
     4561 tggggtttgt tccctcgatg gttgccgctt ttgctgccag cggctttaac aggctgttgc
     4621 ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac ggtcgggata
     4681 aaaggcacat ctgacgcgca tcatcaaaga cgatccgcgc ctggtcacgg ctcaccgctg
     4741 cggtgtaaat atcctgctga ccgttctcca taatcagaaa ccagttagcc agaatcgcgg
     4801 cggtcgttga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg tatttgcgcc
     4861 gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc tgccacggct
     4921 ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc ctggaaaagg
     4981 caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta agatccgaaa
     5041 agtaccgttt aacggcctgt ttcagccgtt tacaggccgg aattttgccc gtttttacgt
     5101 cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc cggtggattt
     5161 ttacggcggc ttatcgggtc aaaaccaagc aaggaggcca tttttatcat cactctttca
     5221 gcgtcggatt tcgcgcttaa tgcggggttt ctgctctcgc cgccctgact gttaacaatg
     5281 ctgaacccgc gcgccgcaag gtctgcgacg gctttgcggt atatcgagta attgacgcaa
     5341 tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc aagctgccgc
     5401 gatttttccc gccactgctt cacggcgatg tcatccaggt aggcgggggc tttcggtggt
     5461 cttgccatgc ttatttttcg tcagattatt tttcaaaaaa ttcccgtgca taaaaatttg
     5521 gggaggcgtt cggtgcccgg cggggtcggg tttgtcctga aaacgccccc caccccgtca
     5581 tacagcctca tcagcgattg cggaaacatt ccataacctc gcggtcacgg tcggttaatc
     5641 gcttcgctgt gatgcgttct gcgcgtcctg acgctttatc tttatgccct gtttcctgtg
     5701 tcttccatgc gtcacgctgc cttataagtc cacggataag gcgtttttgt tcccgctcat
     5761 tcatcagcgt catacatcca gttattgcgg ttagcggccc gttcttcctc ttcacgaaat
     5821 ccacctgcgg cacgcttgct ttttgttgct ggatcaagcc acttcgtttt ctggttatga
     5881 cacgcctggc acaatggctg atggttccat tcgggccaga agagaacatc atcaccgcca
     5941 ttaatcggga taatgtgatc caccaccacg gcgggcgtat atatcccctt ctcaaggcat
     6001 cgcacgcata acgggttttt actcagatac atggcgcggt atttttccca ctgtctggag
     6061 tacccacgcg cgcggcggtg tcctcgtctg gcatcctctg cacgccatgc cgcccgcctg
     6121 tgctcctcac acttgccgga cttaacgcgc ctgttacagc ctggttctgt acatcgtctt
     6181 aatggttgcc acggcatcag tacaccccca catcacggta agccgtccag agcgcgccaa
     6241 tcgtcatggg tacgcgcgtt tttgcgttat ccgctaccag ctggcgattc tcgtacaggt
     6301 gagcgataaa catcatgcag ccaatcttta tggctggcgt gaactccagc ccgtcatcaa
     6361 agcgcctgcc tatatgtatc tggcacgcct caagcgatgc ggcaatgtat ccgctgatta
     6421 actggtcttc ctcgtcgcca tcgatgcggc aatggagttt cacttcttcc agggtgataa
     6481 gttcttctgt catttttccg cgccctcacg acaaagaatt tcaaggcgtg tcctggcggc
     6541 atccggcagc ggttgcccga tgatattcag tacacgcccc gccagcggcc ccgtattgac
     6601 ctttatccgg ctggtggcgt tgatgtcctt ccggtaccgt atccagatcc ttacggtacc
     6661 aatcgccagc tctgcacccg atgaaatggc ctccttgctg ctgatcatgt tcacgcttgc
     6721 ccagagtgtg tgaccgtcct cccacgtttc gagtatttcg ccggttatat ccctggtctg
     6781 tttcagggtc tgaatcgtta ccctgtcacg cagtcgccct atgttcattc cggtttttct
     6841 ccctcgctga tttttacttc ctgtttccat gcctggctga actcatcacc accatcacgc
     6901 ggggacagcc cttcacgttc gcgggcttcg ttcgggcaca taacgccgga tttgatgccg
     6961 cgttcatagg tggcaaagcg ttcgccaggt gtggcccgta acaggtccgc gctgtcaaac
     7021 tccacctgat accggatacc aggtacaggc gaggccacca gcagggcgtt ttttatctgc
     7081 tgctcaaagt tcgccagcca cgggcgcatt gtcatggtga gaaatgcgcg gcttgcctcg
     7141 ctgaaattgc tgtaggtgct gttgctgtat tcctgcagaa aaatcggcga gacgttgaac
     7201 attcgggcga tgtcttcaat ggtgaagcga cgggaggcca gccattcagc atcctgatta
     7261 ctcatgccca gctgctgata gctcataccc ccttcaagaa tgggcgtttt tccggcgttt
     7321 ctggctcctt tgtagcgttc cagtgcggct aatgcctgtt tgcccttcac gccgtccagc
     7381 cattcgcctg acgtgataac ccctgccgcc atcatgccat cacgcatcac gctcgcgccg
     7441 tggcgttgtt gggccagccc aagccccagc gattcgcggc agatggttac aggtgagcgc
     7501 cccataaaac cgtcatccgt ggagtaacga aggtggagaa cctcccacgg taaatagttg
     7561 cgggtgtttc cggtgtaggc gtcagtgatg cagtagcgcc agttatgctc acctgtctgc
     7621 tcaacgttca ccgactgcgg cgggtaagga tgtaaagcca ccggaaaacc atcacgcccc
     7681 cactgaatca ccgcataagc attaccgttt aacaggcagt ggcggatcat cattcgctta
     7741 aactggtagg gagtctgcca cgcgttcggg cgctcgttga ggatgtgatc gaccggatga
     7801 gaatcaagcc actcgcgggc ctccttcccc ttctcattgc gtaccaggta caggtaacac
     7861 ggcatggtag ccaccgcctc agagatgacc gtcacggcgt tcatgacggc gggcagtgat
     7921 tccgctgtcc ccgatgatac gtactcaccc gcccccgtgt tcgatgtgcc agccagcgcc
     7981 ataaattcat caagcgtcat gctgcgctgc tctttttttc ttctgaaaag ccacatatca
     8041 cacccccgct aaatccaccc accagcggcg attatccgca cgcggcattt tttctgggtg
     8101 ctgttcatac agggaacggc gggccagctc cacgccggaa tcggggtaag ccggtaccga
     8161 tgtaacggta atttcgtaca gttccgccac cagcacggtg cgcacgcatg gatctgttgt
     8221 ggtatcccat acatccttac gggagcgaaa gccaaagctc atccccgaga tgtccccacg
     8281 cttaaccagt tcgataacgt cgcgccctgt actggtatcc ggtggggtca gttcaaagcg
     8341 taaccctgtt tcgtcctctt ccagtttcag cgtgccggaa cgggtacgcc ccagcaacat
     8401 gctgtggtca tgctcataca ggccgcgaac gtcattaccc accgcaagcc attcagtaaa
     8461 cgctccccgc tggaattttt cgtaaaactc cccccatagc agctctgaaa gcttatccca
     8521 gcgaacaacg tagcctgtaa gcgtgttgct ggcgctggtg gtgatttccg atgaccggat
     8581 ttccatactc ttcataattt tttcacccat aaaacactaa aggggctttt aagccccttc
     8641 tgtatgctgt taatcgtcgt cctgtggcag ttccagaatc ttgatcgcgt tcgaatccac
     8701 cacgccaccg cctaaatatt tctgcgtgaa aattttgatg aagcccggct ctgtgaggtt
     8761 gtccggtctg gtgcgaacac ctgtttcgtg atcaacaatg tagtaaccgc gcttgaagtc
     8821 acccagggca ataacgttat caggcataaa ctccagatat tcgaccggaa ggcccagcaa
     8881 cgtatcagga tcacccgcct gtaaacggtc gcgccagatg taatcaccgt tcgcgttctt
     8941 caccttctga agttttgccg ccgtcgtgga gttaaccacc cagaccgcgt ttttgcggta
     9001 ttttttacgt aatgcaaatt tcaggtcgat cagcgggtcc gcagatgtcc acgccagaga
     9061 ttcggaaggt ttaattacct gcaacgtacc aaaatcacgc tctttgtcgt tcttctctgc
     9121 acggggtacg gataaaaaac cttttgcttt tttgtcaccg tcgcccacaa ccagatcgct
     9181 ttcttcggtt tccgtgaagg tgtcgccaat ctcacccgtc agccatgaaa ggatgtccac
     9241 atcggaaaaa tccacgattt cctgtgtggt gcgcgggtac gcatagaccg gatacagctt
     9301 aatgctcacc tcgttaatct gcggggtgct ggtctgttcg cgtgccttac cctcttcacc
     9361 gtggttaacg gtcacaccgc cagcggaaac aagctgctta aactcgttgc tgctgatttt
     9421 tttcacggta cagatgcggc gcatggtgga ctcatccgcc agcattcgca tgatttcggt
     9481 gttcagttcg gggataacgg tataaccacc atcagcggga acgcctgtac ttaatgcgcg
     9541 ggtttcacct gtcagaatgt agctgcgtag ttctgcgggg tcagtggtct ggctgctttt
     9601 acctggcttg ctgcgctctt cgtctgcaat ggcttcaagg cgggaaatgt ctttatcgag
     9661 ggattcagct ttagcgcgta attcgtcaaa ttttgcgccc tcagcatcgt taagactgcg
     9721 gttttctttt tcagcgttct ccagcatgtc gcgcatctga tttttaatgg cggttttctg
     9781 ctggcgtaat tcgattattc tcggcataaa aaaaagtcct ggggttaagt aaggaactcc
     9841 aggacgcggc aaacactcaa ccgtttttca taaggaaatc agcaatcgca ccgatcgttt
     9901 tcccgcctgg taatgaatat tggcgagcac attaacaggc gggaaagtgg ccccagcgtc
     9961 ctggcaccac gggcgagaat aatcatgatt cagttcgggt aaaatatgcc gatccgctca
    10021 gtgaacaacg tggaacaacc acgaacaaat aatttacaaa aaataacaaa aagccggatt
    10081 gctccggctg tatgtgctgt atgggtgctc agtctctgat c
//
LOCUS       DAJVTY010000149        10047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVTY010000149 DAJVTY010000000
VERSION     DAJVTY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.149"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..1717)
                     /gene="espL1"
                     /locus_tag="OQQ66_003316"
                     /pseudo
     CDS             complement(1..1717)
                     /gene="espL1"
                     /locus_tag="OQQ66_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            2239..4167
                     /gene="thrS"
                     /locus_tag="OQQ66_003317"
     CDS             2239..4167
                     /gene="thrS"
                     /locus_tag="OQQ66_003317"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1906126.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4171..4713
                     /gene="infC"
                     /locus_tag="OQQ66_003318"
     CDS             4171..4713
                     /gene="infC"
                     /locus_tag="OQQ66_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1906127.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4810..5007
                     /gene="rpmI"
                     /locus_tag="OQQ66_003319"
     CDS             4810..5007
                     /gene="rpmI"
                     /locus_tag="OQQ66_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1906128.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5060..5416
                     /gene="rplT"
                     /locus_tag="OQQ66_003320"
     CDS             5060..5416
                     /gene="rplT"
                     /locus_tag="OQQ66_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1906129.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5533..5657
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5539..5583
                     /gene="pheM"
                     /locus_tag="OQQ66_003321"
     CDS             5539..5583
                     /gene="pheM"
                     /locus_tag="OQQ66_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1906130.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5866..6849
                     /gene="pheS"
                     /locus_tag="OQQ66_003322"
     CDS             5866..6849
                     /gene="pheS"
                     /locus_tag="OQQ66_003322"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1906131.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6864..9251
                     /gene="pheT"
                     /locus_tag="OQQ66_003323"
     CDS             6864..9251
                     /gene="pheT"
                     /locus_tag="OQQ66_003323"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1906132.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9256..9555
                     /gene="ihfA"
                     /locus_tag="OQQ66_003324"
     CDS             9256..9555
                     /gene="ihfA"
                     /locus_tag="OQQ66_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1906133.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(9859..9999)
                     /locus_tag="OQQ66_003325"
     CDS             complement(9859..9999)
                     /locus_tag="OQQ66_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCS1906134.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2640 a         2282 c         2580 g         2545 t
ORIGIN      
        1 tatttttttg caaaaatatt cagcgtcgat atataagata acacgacgtc ttcattaccc
       61 ttgctcatgg cgatgtataa tacaggggtt ccatgtgctg tagcgccttt taataaatcc
      121 atgatgttag ctttgctcaa cttgtattta aaggcgatgc cattaatttt agagaggaac
      181 cgtgtgacac acaaagggtg attattttcc attgcagcaa acaaccctag ttcaccatca
      241 cgtgttttca ttgctaaaat atccatcact atttttttgt ttaaaagctc ttgtgctatt
      301 ggagtggtta atgcattcag aaagatatcg attgtttcat cgaacccata caacatggag
      361 atatagagcc caggtgtttc gttagcactt ttagtttgca gtaatttaac cagattatct
      421 tcatgaatga tatgattgta tatcagtgac tgaatttctt gcataaatat ttttacgttg
      481 tcagtatgtc cgttcatcat agccagaaat aaagcaggtg taccaacgct attctgcgca
      541 gttattattt caatgatttc tttttcaggg agtatgggca actgtttgaa taatgtaata
      601 agctgttgag tctggcctgt ggccattatt aacaacattg ccgatggatg tataggggca
      661 aatggcattc gtgatgcaat acccgcgaaa atagcccctg gaactcccac aggtaatagg
      721 ggcagaatag caattgcaca gttgcgaaca tacctatcag cccaggtctg agcatcggga
      781 cctgaactga tgaacttatc aagcgaaaga aagccccgtg ctgtcctgtg ggtgcctctg
      841 acggcaacgt tggtatcgtt cgggtcgtag acggacacta catagtttaa ttgtccatac
      901 actaactgct ttattcgtag ccttactgac aaagtatgat tgtctatata taaaatcgcg
      961 gacatatgac tgacattgtt tgccatcatt cgacaaaaga aaatatctaa agcgaagcca
     1021 aaatccttac tcgcgataac gcgactccct gcaggaggat ggcgaagtaa tgctctgaac
     1081 tccgcatccc ggactaaggg gacatgctgc tgtatggatt caacggagga gtaatgttta
     1141 atcaggttga ctttgccttt tccatttctc aatgaatcaa gggcgtattg caatgacaga
     1201 tggcgacaaa aaatagcgtc agaaggagat tgcaaaaaac atgcactacc attgagattt
     1261 actgttggtt gtatagtttt ttctctatgg atttttttag acagtttcat acaatggtta
     1321 cgatcttcgg cagttaactg ttcctgctca atataatcca gtatgcttcg caaatggtta
     1381 gccttaatag gcgtggtttc cagaaataag ggaatgattt cttttttata gtgtaatgta
     1441 ataagtatat tttctgcatg gatgggtgaa gtggcaaagt taatgataaa aagggcagtg
     1501 gagtaacaat ttgtgatatt tttcgacagt ttgcggtaaa ttcctgagta atacagtatg
     1561 acagcatcga acttaagtca gaggtatcaa gtagggtgat gagttggcgc gcaaattgat
     1621 taataagctc tgatgaaggc gttggtatgt cgctatcgat gtgcaatcta tgaggagact
     1681 tataatgagt tctgataatg atatcgtttt gactcataat ctattcacct tatatatact
     1741 ggaggttttg aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg
     1801 ctataagaat tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga
     1861 tcgtttaaat actaccgcca aatcacgcaa cgaactttgc tggattcagt ttatgttgct
     1921 gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat gttatcagtg gttctttaag
     1981 cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag
     2041 gtacaatctc ccttttttta gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa
     2101 tgtctttcat ttgtgtacct gattgcgaac caatttagca tttgttggct agatggtttc
     2161 gcaatgaact gttaataaac aaatttttct ttgtatgtga tctttcgtgt gggtcaccac
     2221 tgcaaataag gatataaaat gcctgttata actcttcctg atggcagcca acgccattac
     2281 gaccacgctg taagccccat ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc
     2341 tgtatcgcag ggcgcgttaa tggcgaactg gttgatgctt gcgatctgat tgaaaacgac
     2401 gcacaactgt cgatcattac cgccaaagac gaagaaggtc tggagatcat tcgtcactcc
     2461 tgtgcgcacc tgttagggca tgcgattaaa caactttggc cgcataccaa aatggcaatc
     2521 ggcccggtta ttgacaacgg tttttattac gacgttgatc ttgaccgcac gttaacccag
     2581 gaagatgtcg aagcactcga gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc
     2641 attaagaaga aagtcagctg gcacgcagcg cgtgaaactt tcgccaaccg tggggagagc
     2701 tacaaagtct ccattcttga cgaaaacatc gcccatgatg acaagccagg tctgtacttc
     2761 catgaagaat atgtcgatat gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat
     2821 catttcaaac taatgaaaac ggcaggggct tactggcgtg gcgacagcaa caacaaaatg
     2881 ttgcaacgta tttacggtac ggcgtgggca gacaaaaaag cacttaacgc ttacctgcag
     2941 cgcctggaag aagccgcgaa acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac
     3001 catatgcagg aagaagcgcc gggtatggta ttctggcaca acgacggctg gaccatcttc
     3061 cgtgaactgg aagtgtttgt tcgttctaaa ctgaaagagt accagtatca ggaagttaaa
     3121 ggtccgttca tgatggaccg tgtcctgtgg gaaaaaaccg gtcactggga caactacaaa
     3181 gatgcaatgt tcaccacatc ttctgagaac cgtgaatact gcattaagcc gatgaactgc
     3241 ccgggtcacg tacaaatttt caaccagggg ctgaagtctt atcgcgatct gccgctgcgt
     3301 atggccgagt ttggtagctg ccaccgtaac gagccgtcag gttcgctgca tggcctgatg
     3361 cgcgtgcgtg gatttaccca agatgacgcg catatcttct gtactgaaga acaaattcgc
     3421 gatgaagtta acggatgtat ccgtttagtc tatgatatgt acagcacttt tggcttcgag
     3481 aagatcgtcg tcaaactctc cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg
     3541 gatcgtgctg aggcggacct ggcggttgcg ctggaagaaa acaacatccc gtttgaatat
     3601 caactgggtg aaggcgcttt ctacggtccg aaaattgaat ttaccctgta tgactgcctc
     3661 gatcgtgcat ggcagtgcgg tacagtacag ctggacttct ccttgccgtc tcgtctgagc
     3721 gcctcctatg tgggcgaaga caacgagcgt aaggtaccgg taatgattca ccgcgcaatt
     3781 ctggggtcga tggaacgttt catcggtatc ctgaccgaag aattcgctgg tttcttcccg
     3841 acctggcttg cgccagttca ggttgttatc atgaatatta ccgattcaca gtctgaatac
     3901 gttaacgaat tgacgcaaaa actatcaaat gcgggcattc gtgttaaagc agacttgaga
     3961 aatgagaaga ttggctttaa aatccgcgag cacactttgc gtcgcgtccc atatatgctg
     4021 gtctgtggtg ataaagaggt ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa
     4081 gacctgggaa gcatggacgt aaatgaagtg atcgagaagt tgcaacaaga gattcgcagc
     4141 cgcagtctta aacaattgga ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg
     4201 cgccctaacc gtatcaatgg cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa
     4261 ggcgagcagc ttggtattgt gagtctgaga gaagctctgg agaaagcaga agaagccgga
     4321 gtagacttag tcgagatcag ccctaacgcc gagccgccgg tttgtcgtat aatggattac
     4381 ggcaaattcc tctatgaaaa gagcaagtct tctaaggaac agaagaaaaa gcaaagagtt
     4441 atccaggtta aggaaattaa attccgtcct ggtacagatg aaggcgacta tcaggtaaaa
     4501 ctccgcagcc tgattcgctt tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc
     4561 cgcggtcgtg agatggcgca ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac
     4621 gatttgcaag aactggcagt ggtcgaatcc ttcccaacga agatcgaagg ccgccagatg
     4681 atcatggtgc tcgctcctaa gaagaaacag taaggccttc aagtagcact gtctgtggag
     4741 ccttcgggct tcacaggctt tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa
     4801 gttattaaaa tgccaaaaat taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc
     4861 ggtaaaggtg gttttaagca caagcacgct aacctgcgtc acattctgac caaaaaagcg
     4921 accaaacgta aacgtcacct gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg
     4981 gtaatcgcgt gcctgccgta cgcataagcc gttaacgttt ttaacttttt aattagaata
     5041 tagatacagg agagcacata tggctcgcgt aaaacgtggt gttattgcac gcgcacgtca
     5101 caagaaaatt ttgaaacaag ctaaaggcta ctacggtgcg cgttctcgcg tataccgcgt
     5161 tgccttccag gctgttatca aagctggtca gtatgcttac cgtgaccgtc gtcaacgtaa
     5221 gcgtcagttc cgtcaactgt ggattgcacg tatcaacgca gcagcacgtc agaacggtat
     5281 ttcttacagc aaattcatca atggcctgaa aaaagcctct gttgaaatcg accgtaagat
     5341 cctggctgat atcgcagtat tcgacaaagt agcgttcacc gctctggttg aaaaagcgaa
     5401 agcagctctg gcataagcca gttgaaagag ggagctagtc tccctctttt cgtttcaacg
     5461 ccatcaaaac attgactttt atcgccgtag ccttttcaat aaaggtcttt tgaagagtac
     5521 caaaaggtaa cgcaagcaat gaatgctgct attttccgct tcttttttta ctttagcacc
     5581 tgaatccagg aggctagcgc gtgagaagag aaacggaaaa cagcgcctga aagcctccca
     5641 gtggaggctt tttttgtatg cgcgttttga aaaataaaaa gccagtctat caggcgtaat
     5701 tgtcgcagcc agtctggata cggccagcgc ggagaaaccg gagcgtattt aagtacgtga
     5761 gaatttcgag cactgcccgg gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc
     5821 taagtccaac gaaccagtgt caccactgac acaatgagga aaaccatgtc acatctcgca
     5881 gaactggttg ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc cgcgttagat
     5941 aatgtgcgcg tcgaatattt gggtaaaaaa gggcacttaa cccttcagat gacgaccctg
     6001 cgtgagctgc cgccagaaga gcgtccggca gctggtgcgg ttatcaacga agcgaaagag
     6061 caggttcagc aggcgctgaa tgcgcgtaaa gcggaactgg aaagcgcagc gctgaatgcg
     6121 cgtctggcgg cggaaacgat tgatgtctcc ctgccaggtc gtcgcattga aaacggcggt
     6181 ctgcatccgg ttacccgtac catcgaccgt atcgaaagtt tcttcggtga gcttggcttt
     6241 accgtggcaa ccgggccgga aatcgaagac gattatcata acttcgatgc tctgaacatt
     6301 cctggtcacc acccggcgcg cgctgaccac gacactttct ggtttgacac tacccgcctg
     6361 ctgcgtaccc agacctctgg cgtacagatc cgcaccatga aagcccagca gccaccgatt
     6421 cgtatcatcg cgcctggccg cgtttatcgt aacgactacg accagactca cacgccgatg
     6481 ttccatcaga tggaaggtct gattgttgat accaacatca gctttaccaa cctgaaaggc
     6541 actctgcacg atttcctgcg taacttcttt gaggaagatt tgcagattcg cttccgtcct
     6601 tcctacttcc cgttcaccga gccttctgca gaagttgacg tcatgggtaa aaacggtaaa
     6661 tggctggaag tgctgggttg cgggatggtg catccgaacg tgctgcgtaa cgttggcatc
     6721 gacccggaag tttactctgg tttcgccttc gggatgggga tggagcgtct gactatgttg
     6781 cgttacggcg tcaccgacct gcgttcattc ttcgaaaacg atctgcgttt cctcaaacag
     6841 tttaaataag gcaggaatag attatgaaat tcagtgaact gtggttacgc gaatgggtga
     6901 acccggcgat tgatagcgat gcgctggcga atcaaatcac tatggcgggc ctggaagttg
     6961 acggtgtaga accggttgcc ggtagcttcc acggcgtggt cgttggtgaa gtggttgagt
     7021 gtgcgcagca tccgaacgct gacaaactgc gtgtgacaaa agtgaatgtc ggcggcgatc
     7081 gcctgctgga catcgtctgt ggtgcgccaa actgccgtca gggcctgcgt gtggcggtag
     7141 ctaccattgg tgctgttctg ccgggtgatt tcaaaattaa agcggcgaaa ctgcgtggcg
     7201 aaccgtctga agggatgctg tgctccttct ctgaactggg aatttctgac gatcacagcg
     7261 gcattatcga actgcctgca gatgcgccga ttggcactga catccgcgaa tacctgaaac
     7321 tcgatgacaa caccatcgaa atcagcgtaa cgccaaaccg tgccgactgc ttaggtatca
     7381 ttggtgttgc gcgtgacgtt gccgtgctga atcagctgcc gctggttgaa ccggaaatcg
     7441 ttccggttgg tgcgaccatc gacgacacgt tgccgattac agtcgaagcg cctgaagcct
     7501 gcccgcgcta tcttggccgt gtggtaaaag gcattaacgt taaagcgcca actccgctgt
     7561 ggatgaaaga aaaactgcgt cgttgcggga tccgttctat cgatgcagtt gttgacgtca
     7621 ccaactatgt actgctcgaa ctgggccagc cgatgcacgc tttcgataaa gatcgcattg
     7681 aaggcggcat tgtggtgcgg atggcaaaag agggcgaaac gctggtgctg cttgatggca
     7741 ctgaagcgaa gctgaatgct gacactctgg tcatcgccga ccacaacaag gcgctggcga
     7801 tgggcggcat ctttggtggc gaacactctg gcgtgaatga cgaaacgcaa aacgtcttgc
     7861 tggaatgcgc tttcttcagc ccgctgtcta tcaccggtcg tgctcgtcgt catggcctgc
     7921 atactgatgc gtctcaccgt tatgagcgtg gcgttgatcc ggcactgcag cacaaagcga
     7981 tggaacgtgc gacccgtctg ctgatcgaca tctgcggtgg tgaggctggt ccggtaattg
     8041 atatcaccaa cgaagcaacg ctgccgaagc gtgcaaccat cactttacgt cgtagcaaac
     8101 tggattgcct gatcggccat catattgctg atgaacaggt aactgacatt ctgcgtcgtc
     8161 tcggctgcga agtgaccgaa ggcaaagacg agtggcaggc agttgcgccg agctggcgtt
     8221 tcgatatgga gattgaagaa gatctggtcg aagaagtcgc gcgtgtttac ggctacaaca
     8281 acatcccgga tgagccggta caggcaagcc tgattatggg tactcaccgt gaagctgacc
     8341 tgtcgctcaa gcgcgtgaaa acgctgctca acgacaaagg ctatcaggaa gtgatcacct
     8401 acagcttcgt tgatccgaaa gtgcagcaga tgatccatcc aggcgttgaa gccttactgc
     8461 tgccaagccc gatctctgtt gaaatgtcag caatgcgtct ttctctgtgg accggcctgc
     8521 tggcaaccgt ggtgtacaac cagaaccgtc agcagaaccg tgtgcgcatt ttcgaaagcg
     8581 gtctgcgttt cgtaccagat actcaggcac cgttgggcat tcgtcaggat ctgatgttag
     8641 ccggtgtgat ttgcggtaac cgttacgaag agcactggaa cctggcaaaa gagaccgttg
     8701 atttctatga tttgaaaggc gatcttgaat ccgttctcga cctgaccggt aaactgaatg
     8761 aggttgagtt ccgtgcagaa gcgaatccgg cactgcatcc ggggcaatcc gcagcgattt
     8821 atctgaaagg ggaacgtatt ggttttgttg gagttgttca tcctgaactg gaacgtaaac
     8881 tggatcttaa cggtcgcact ctggtgttcg aactggagtg gaacaagctc gcagaccgcg
     8941 tggtgcctca ggcgcgcgag atttctcgct tcccggcgaa ccgtcgtgac atcgcggtgg
     9001 tggtcgcaga aaacgttccc gcagcggata ttttatccga atgtaagaaa gttggcgtaa
     9061 atcaggtagt tggcgtaaac ttatttgacg tgtaccgcgg taagggtgtt gcggaggggt
     9121 ataagagcct cgccataagc ctgatcctgc aagataccag ccgtacactc gaagaagagg
     9181 agattgccgc taccgtcgcc aaatgtgtag aggcattaaa agagcgattc caggcatcat
     9241 tgagggattg aacctatggc gcttacaaaa gctgaaatgt cagaatatct gtttgataag
     9301 cttgggctta gcaagcggga tgccaaagaa ctggttgaac tgtttttcga agagatccgt
     9361 cgcgctctgg aaaacggcga acaggtgaaa ctctctggtt ttggtaactt cgatctgcgt
     9421 gataagaatc aacgcccggg acgtaacccg aaaacgggcg aggatattcc cattacagca
     9481 cggcgcgtgg tgaccttcag acccgggcag aagttaaaaa gccgggtcga aaacgcttcg
     9541 cccaaagacg agtaatctga tctaactaaa aaggccgctc tgcggccttt tttctttcaa
     9601 taactaattg atttataatg ttttatatct cttcgttcca cgatgcgtcc acatttagtg
     9661 gggggagggg agtaagcccg ctacattgtg ggctttgtct tatagtctga tcactgtttc
     9721 aagtcggtga ggacaccaaa aagaaccccc gcagttttta cgctgcgggt ttgttgttca
     9781 tgtctgtgag atagggtgcc ttatcgacct taccctggca accgattgac gggggattgc
     9841 tcccccgtcg cggtttcctt actgcttaca ctgtaagaac gccgcaaact ccgctcccca
     9901 gaagctcatc cgtatttcgc acagcgaacc gtgcagcatc cagatgatga ggattgccgt
     9961 cacgcagaac gtgatggccg taagcgattt ttgcgacata gcgcttgctc ctttttcggg
    10021 gaggcgctaa cctatcactt gcttagg
//
LOCUS       DAJVTY010000150        10033 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVTY010000150 DAJVTY010000000
VERSION     DAJVTY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.150"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OQQ66_003326"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OQQ66_003326"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1906135.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OQQ66_003327"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OQQ66_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1906136.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OQQ66_003328"
     CDS             1309..1830
                     /locus_tag="OQQ66_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1906137.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OQQ66_003329"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OQQ66_003329"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1906138.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OQQ66_003330"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OQQ66_003330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1906139.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OQQ66_003331"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OQQ66_003331"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1906140.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OQQ66_003332"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OQQ66_003332"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1906141.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OQQ66_003333"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OQQ66_003333"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1906142.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OQQ66_003334"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OQQ66_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OQQ66_003335"
     CDS             complement(7360..8175)
                     /locus_tag="OQQ66_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1906143.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OQQ66_003336"
     CDS             complement(8186..9445)
                     /locus_tag="OQQ66_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1906144.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10033)
                     /locus_tag="OQQ66_003337"
                     /pseudo
     CDS             complement(9455..10033)
                     /locus_tag="OQQ66_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="multicopy suppressor of dominant negative ftsH
                     mutations; putative acyl-CoA synthetase; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2203 a         2845 c         2694 g         2291 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgatttcac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgtgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgcggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtg
//
LOCUS       DAJVTY010000151         9912 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVTY010000151 DAJVTY010000000
VERSION     DAJVTY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.151"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            19..171
                     /gene="hokA"
                     /locus_tag="OQQ66_003338"
     CDS             19..171
                     /gene="hokA"
                     /locus_tag="OQQ66_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1906145.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(359..571)
                     /gene="cspA"
                     /locus_tag="OQQ66_003339"
     CDS             complement(359..571)
                     /gene="cspA"
                     /locus_tag="OQQ66_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1906146.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(852..1142)
                     /locus_tag="OQQ66_003340"
     CDS             complement(852..1142)
                     /locus_tag="OQQ66_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS1906147.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1575..2285
                     /locus_tag="OQQ66_003341"
     CDS             1575..2285
                     /locus_tag="OQQ66_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1906148.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2335..3309)
                     /gene="ghrB"
                     /locus_tag="OQQ66_003342"
     CDS             complement(2335..3309)
                     /gene="ghrB"
                     /locus_tag="OQQ66_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1906149.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3413..4072)
                     /locus_tag="OQQ66_003343"
     CDS             complement(3413..4072)
                     /locus_tag="OQQ66_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1906150.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4225..6558
                     /gene="bisC"
                     /locus_tag="OQQ66_003344"
     CDS             4225..6558
                     /gene="bisC"
                     /locus_tag="OQQ66_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1906151.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6527..6967)
                     /locus_tag="OQQ66_003345"
     CDS             complement(6527..6967)
                     /locus_tag="OQQ66_003345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1906152.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6964..7527)
                     /gene="tag"
                     /locus_tag="OQQ66_003346"
     CDS             complement(6964..7527)
                     /gene="tag"
                     /locus_tag="OQQ66_003346"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1906153.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7685..8383
                     /locus_tag="OQQ66_003347"
     CDS             7685..8383
                     /locus_tag="OQQ66_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1906154.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8611..9813
                     /locus_tag="OQQ66_003348"
     CDS             8611..9813
                     /locus_tag="OQQ66_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906155.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2358 a         2397 c         2552 g         2605 t
ORIGIN      
        1 caacacaggg ggctgggaat gccgcaaaaa tacggattac tttcattaat agtgatttgt
       61 ttcacgcttt tatttttcac ctggatggta agagattcac tgtgtgaatt gcatattaaa
      121 caggggcgtt atgagctggc ggcattttta gcctgcaatt taaaagagta agagtcttcg
      181 gcgggaaatt attcccgcct ttcttacggc gttgcgcatt ctcattgcac ccacatttat
      241 tcttcacaaa aataataata gattttacta cgcgatcgat tatttatttc ctgaaaaaaa
      301 ataaaaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta agcagagatt
      361 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
      421 ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga
      481 acacatcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
      541 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc
      601 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
      661 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
      721 gtcaaaccgt tgatgtgcat taagccacgc attggcgggt gatgcaacaa ttatttttca
      781 tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag
      841 tggataaaag tctattccat caactgctta cttaatgccg ggttggcttg aatcaaacgc
      901 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
      961 gcgactgata tgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt cccttttcgt
     1021 aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
     1081 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
     1141 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
     1201 agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta tgattcgatt
     1261 cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
     1321 cttaccactc ctgttttgta aagatttcga aaatctaaag ttctgcgaag gcgtaaatct
     1381 cgggctacaa aaaagcaacg gcaatatcat tttttaacaa tgaaacaaca aattaacctt
     1441 gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc ctgcaagcct
     1501 gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg
     1561 aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg
     1621 cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat cagcgtaaag
     1681 cgtttatcga cttcctgcag aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga
     1741 ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt
     1801 attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggtcgta gacagcgtta
     1861 acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt gaaatgaacg
     1921 gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc gatgctgcgc
     1981 actctgcgct aaaacagacc gatgatttga aaccggtctt tgatcaggca ttcaccaaag
     2041 tggtaacgac gccagctgat gcattgcaac cgttgattcc ggttgcacaa actttcacgc
     2101 aacaactggt gatggttgga gactacattg ctcaacaagg tactcaggtg agctttgtag
     2161 caaatggtat ccagttcccg acttcgcagc aggcaagtga atataacaaa ctgattgcgc
     2221 cgttaccggc acaacatcag gcgtttaatc aggcctggac tacagcagtt actgcaaccc
     2281 aataaagagt aaaagcccga gcgattatcg ggctttactt cacgcagtcg cggcttagtc
     2341 cgcgacgtgc ggattcacac agttcttctc aacctttcct tgtaacgcat caatcaaatt
     2401 atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc caatatgcgg
     2461 tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggacagtg gctcttgttc
     2521 gaagacatcc agcccggcgg cgtgaatttc gcctttctgc aatgctgcga tcagtgcatt
     2581 ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggagggtt tcattttggc
     2641 gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga tcaggcaaac
     2701 gaaatctgac tcttgtaaca gagtatccaa atcgcagtag cgggcgttga agcgttcttc
     2761 tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc caaagtgcgc
     2821 acgttgtgcc agcgccatgc cgatccgtcc catcccgaca atgcccagtg ttttatggtg
     2881 aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg cttttacccg
     2941 ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca gcgtatcggc
     3001 gacggtttct gttaatacgg ttggcgtgtg catcagcaga atttttcggg cggtaagcgc
     3061 atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca gtttcggcat
     3121 tttttccagc aatgcggcat ctacattctc gtttgaaccc agtaaacctt cagcttcggc
     3181 aaaaattgct gcattttgtt cgacggtttg tgggctgagg tttgccacct ggtgaacggt
     3241 gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga ggataacgga
     3301 cggcttcatg cttctctcca ttcactgata aggcgctcac cttagcctga aatatgatga
     3361 aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg gattacagcg
     3421 ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg
     3481 cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc acgccctggg
     3541 tgatcaacgc gctggcaacg gaatccgcac gctgctggga gagacgcatg ttcaggtcgt
     3601 gaccccccgt gctgtcggta taaccaatca cgttaaccgc cgttttcgga tactctttca
     3661 gcaccattgc cacgccggtc agggtgttag cgcctgccgg tttcagggtc gcgctgctgc
     3721 tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg cgggttacgc
     3781 taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac
     3841 ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat
     3901 ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagtcc agacctgcgc
     3961 cgatagcaga tttgcctgct tcgcgttcgc cggtgtaagg gttagttgtg cagccagata
     4021 ccgccagagc gccactcact acggcggcaa taagataaac acgtttcttc attgttaatc
     4081 cttaataacc tttttattct ttgccacggg ttccgtggcg ggagattatg ccgcgtgaac
     4141 atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca
     4201 tctaaccttt caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact
     4261 gccgcccact gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt
     4321 ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac
     4381 agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac
     4441 ccgcaaggca ttcgtgggca ggatgaattt gttcacgtga gttgggatga ggcgctggat
     4501 cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc gatttttgct
     4561 ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt attacaacgc
     4621 tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac cggtgcggca
     4681 caggcgatca tgccgtatgt cgtgggtggc agcgaagttt atcaacagca aaccagttgg
     4741 ccgctggtgc tggaacatag cgatgtcgtg gtgttgtgga gtgctaaccc actcaatacg
     4801 ctgaaaattg cgtggaatgc atctgatgag caggggcttt cttacttttc cgcactgcgt
     4861 gacagcggga aaaagctgat ctgtattgat ccaatgcgat cggaaaccgt cgatttcttt
     4921 ggcgataaaa tggagtgggt ggcaccgcac atgggcaccg atgttgcgct gatgctgggg
     4981 atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc gcgttgcacc
     5041 acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat agccaaaacc
     5101 gccgaatggg cggcagggat ttgtggggtt cacgcagcga aaattcgcga gttggcggct
     5161 cttttccacc aaaacacgac catgctgatg gctggctggg gaatgcagcg ccaacagctt
     5221 ggtgagcaaa aacactggat gatcgtcacg ctggcggcaa tgttggggca aatcggcaca
     5281 cccggcggcg gttttggtct ttcttatcat tttgccaatg gtggtaatcc cacgcggtgt
     5341 tctgcggtgc tctcttccat gcagggtagc ttgccgggtg gcagcgatgc ggtagataaa
     5401 atccctgttg cccgtattgt tgaagcactg gaaaaccctg gctgcgcata ccaacacaat
     5461 ggcatgaatc gcctcttccc ggatattcgc tttatctggt gggcgggtgg cgccaacttc
     5521 acccatcatc aggacactaa tcgcctgata cgagcttggc aaaaaccgga gctggtagtt
     5581 atctccgaat gcttctggac ggcggcggca aaacacgcgg atatcgttct gcctgcgact
     5641 acctcttttg agcgtaatga tctcaccatg accggcgatt acagtaatca gcatctggtg
     5701 ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga tgtttttgcc
     5761 gagttaagtg aacgctggga gaagggcggt tatgcacgtt ttacggaagg aaaaagtgag
     5821 ttgcaatggc tggaaacgtt ttataacgtt gcccgacagc gcggggcaag ccagcaggtt
     5881 gaattgccgc catttgctga gttctggcaa gccaatcagt taattgagat gccggaaaac
     5941 ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgca ggcgcatcca
     6001 ttaaaaaccg ccagcggtaa gattgaaatc ttctcgcagc gtattgccga ttacgcttat
     6061 ccggattgcc cggggcatcc aatgtggctg gagccggacg aatggcaggg caatgccgaa
     6121 ccggaacagt tacaggtact ttctgctcat ccggcgcatc gtctgcacag ccagctgaat
     6181 tacagttctc tgcgcgaatt gtatgcggtg gcaaatcgtg agcctgtcac cattcatcct
     6241 gacgatgccc tggcgcgcgg cattcaagat ggcgatactg ttcggctgtg gaacgcacgc
     6301 gggcaaattc ttgccggagt ggtcattagc gagggaatta aacctggcgt gatttgtatt
     6361 cacgaagggg catggccgga tttggattta accgctgacg gtatttgtaa aaacggcgca
     6421 gtcaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg tgcgggtaat
     6481 acggcgctgg catggctgga aaaatacaac ggtccggaac tgacacttac agcgtttgaa
     6541 ccaccggcca gctcataatc caggtgggta gttgggtttc atcctgccat gcgcaatcga
     6601 caatgtgaaa accctgcgct tggtaaaaat ttatcgccgg ttgatttttt tgataaacct
     6661 ccagcatcag gtggggatgg cgctgctgca catattgcat cagcgcctta ccaataccgc
     6721 gcctgacggc cttcggtgcg acaaacatcg ctgccagaaa tcggccttcc ataatgctga
     6781 caaaaccgag aagcttactg tcttcttccc agacccagtt ttgcgcattg gcaagatacg
     6841 catcccgcac cagcggaatg caatcacgcc agtaattcgc ttttataaag ggatgtcccc
     6901 aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt tgcgcttccc
     6961 gaatcatggt ttatttcccg gatagcaaca gcagccaacc acatgatcat tcaccagccc
     7021 acatgcctgc ataaaggagt aacagattgt ggtaccgaca aacttaaaac cacgtttttt
     7081 cagtgcttta gatagggcgt cggaggcggg cgtagatgtg gggatttcgc tcaacgttgt
     7141 ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcgg caaacggttc
     7201 gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct gaattttccc
     7261 gcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt cctgcattgc
     7321 tgcgaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc gttttttgag
     7381 gacggtgatc cacgataacc cagcctgctg cccttcaagg cagatcattt cgaacagttt
     7441 tttactgtca gtttcaggca cgccccactc attatcatgg taggcaatat aaagcgggtc
     7501 ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga tcgtaaaaat
     7561 ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag ggctactatg
     7621 cccgatatcc ttcatacaat gacaataata ttgccgaatt cggctcttcc ctggagtcgt
     7681 gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg cgagcgtggt
     7741 aatcagcgcc ttttttctca acacagctta cgcctggcaa caagaatata tcgttgatac
     7801 gcaacccgga cattccacag agcgttacac ctgggatagt gatcatcaac ctgattacaa
     7861 cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct
     7921 ggcggaagaa actcctgtgg atgtgaccag cagtatgagt atgggctgga attttccttt
     7981 atatgaacag gttacaaccg gcccggtcgc ggcattacat tacgatggca caaccacctc
     8041 gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat ggcatgccag
     8101 cgtgagtagc ttaggctggc gtgttgactc ccggcttggc gatctccgac cctgggcgca
     8161 aatcagctat aaccagcaat ttggcgagaa tatctggaag gcgcaatcag gcctgagccg
     8221 gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg ctgatatgtt
     8281 gctcaatcaa aatattgccg cctatgccgc gctaactcag gcagaaaata ccactaataa
     8341 tagcgactat ctgtatacga tgggggttag cgccagattt taacgtaaca gtcacaattg
     8401 aaaccattga ataacaatag ttgtggtgat agtgggtgct aacttacaaa taataaattt
     8461 ggtgaataat tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc
     8521 atttcatgcc gtttttcccc aggcataaaa tgcacttcgt tatggttgtc ggcagagatt
     8581 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc
     8641 tggctgacac tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg
     8701 agcctgttta atggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc
     8761 tctttcggct tgttaagtct ggggctggca atttcatctt ctgttgcggg gaaattacag
     8821 gaacgttttg gcgttaaacg cgtcactatg gcttccggca ttttgctagg attaggcttc
     8881 ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc
     8941 ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg
     9001 gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtcttgg tagcctgggt
     9061 ttcaaattta tcgacacgca gctgctggaa acggtgggtc tggaaaaaac atttgtgatt
     9121 tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca
     9181 aaacaggaag tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg
     9241 atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc
     9301 ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt
     9361 tccgcagcca atgcagtaac ggttatttcc atcgctaacc tttcaggtcg tctggtgctg
     9421 ggcattctgt ctgacaaaat tgcccgtatc cgtgttatca ccattggtca ggtgatttcg
     9481 ctggtgggta tggcggccct gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg
     9541 attgcctgcg tggcatttaa ctttggcggc actattaccg tctttccatc actggtcagt
     9601 gagttcttcg gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt
     9661 atcggtagca tttttggttc gattatcgcc tcactgtttg gcggtttcta tgtgaccttc
     9721 tacgtgattt tcgccctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca
     9781 gagcagaaaa tgttgcgtga ggcgcatatt tagaaatatg ttgctactga aataaatacg
     9841 aggagggaga cttcaaagtc tccctttttt atggacgcat attaaaagaa caataatatt
     9901 cataccacaa at
//
LOCUS       DAJVTY010000152         9896 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVTY010000152 DAJVTY010000000
VERSION     DAJVTY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.152"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQQ66_003349"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQQ66_003349"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1906156.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQQ66_003350"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQQ66_003350"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1906157.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OQQ66_003351"
     CDS             complement(2696..3388)
                     /locus_tag="OQQ66_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCS1906158.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OQQ66_003352"
     CDS             3518..5278
                     /locus_tag="OQQ66_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1906159.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OQQ66_003353"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OQQ66_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1906160.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OQQ66_003354"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OQQ66_003354"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1906161.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OQQ66_003355"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OQQ66_003355"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1906162.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2535 a         2488 c         2355 g         2518 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtttctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgtccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaagat ggaataatcc agtatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgaggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt attcgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcggtggtt gaagcgatcg aaacactgga agcagaaggt ttcccgatct tcgcatatga
     4261 tggttccctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtgga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctaatgctc
     5461 ccttttaatt aactatttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat atttactccg tatttgcata aaaaccatgc
     5581 gagttacgga cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agctgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatctccg gaattctgga ccgctgtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaac ttcatcactg ataacctgat cccggttacg atcggtaata ttatcggcgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg atcaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctagct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacctggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactat gctatcgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atctcctgaa ctacgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcactg aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaatgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaactggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgct gtagcgtctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ttaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga cacgcatggc ggtaaagaag ttaccgttga agatttgatg
     9241 aaggaagtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgaag caatcctgca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaac caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gaactcctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc acctaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgcg cttcattctg ctacagtgtt tttaacacat tttata
//
LOCUS       DAJVTY010000153         9805 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVTY010000153 DAJVTY010000000
VERSION     DAJVTY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.153"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(106..294)
                     /locus_tag="OQQ66_003356"
     CDS             complement(106..294)
                     /locus_tag="OQQ66_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906163.1"
                     /translation="MLTRKSIDPVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            410..1420
                     /locus_tag="OQQ66_003357"
                     /pseudo
     CDS             410..1420
                     /locus_tag="OQQ66_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            complement(1422..1754)
                     /locus_tag="OQQ66_003358"
     CDS             complement(1422..1754)
                     /locus_tag="OQQ66_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1906164.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    1994..2066
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            2060..2473
                     /gene="ibpA"
                     /locus_tag="OQQ66_003359"
     CDS             2060..2473
                     /gene="ibpA"
                     /locus_tag="OQQ66_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1906165.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            2585..3013
                     /gene="ibpB"
                     /locus_tag="OQQ66_003360"
     CDS             2585..3013
                     /gene="ibpB"
                     /locus_tag="OQQ66_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1906166.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            3209..4870
                     /locus_tag="OQQ66_003361"
     CDS             3209..4870
                     /locus_tag="OQQ66_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1906167.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(4867..5583)
                     /locus_tag="OQQ66_003362"
     CDS             complement(4867..5583)
                     /locus_tag="OQQ66_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1906168.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            5878..7494
                     /locus_tag="OQQ66_003363"
     CDS             5878..7494
                     /locus_tag="OQQ66_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1906169.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHALLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            7494..8817
                     /locus_tag="OQQ66_003364"
                     /pseudo
     CDS             7494..8817
                     /locus_tag="OQQ66_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(8948..9379)
                     /gene="tnpA"
                     /locus_tag="OQQ66_003365"
                     /pseudo
     CDS             complement(8948..9379)
                     /gene="tnpA"
                     /locus_tag="OQQ66_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            9387..>9805
                     /locus_tag="OQQ66_003366"
     CDS             9387..>9805
                     /locus_tag="OQQ66_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906170.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRN"
BASE COUNT         2294 a         2449 c         2597 g         2465 t
ORIGIN      
        1 gatatcgcgc agaccgcccg catccaggaa ggatgtccag cactggttgg ccgtgaacag
       61 acgtttcagc gggcggggag taaaagcgga caacatgagg acggattaac ccccggtatc
      121 ctgtagccgg gtaagggctg ctgtgtcgcc acggcgctcc aggatggagg ccgcgaccat
      181 cgcaggtggt gggtccatgg gtttgagcat cttcatccag tcccattcac gttgtgagag
      241 cttctcagca ccaacagaga gcagaacggg atctatactt ttgcgggtca acataagagg
      301 atgagtcctt aataaaacag gtcctcagag catacatatt ctgcggagcg tgcggcaaca
      361 acaggacgaa caggggcggt tttcaatttg cagccgtcag gctgccgtgg ttcatccgaa
      421 gtcagagaaa tatgttttca tccatggccc ggaaaatcct gatgaaacat ggtattacga
      481 tttccatcat cgacgcggag tcattgctga aggcggcaag gtgagcaatc tcgatgcaat
      541 ggatattacc gcgccgtata cgccaggagc gctgcgcggc ggcagccatg tgcatgtctt
      601 tagcccgaac ggtgaaaggg tgagctttac ctataacgac catgtaatgc atgaactcga
      661 tccggcgctg gatttgcgaa acgtcggtgt tgctgcgccg tttggcccgg tcaacgtaca
      721 aaagcagcat ccgcgtgaat acagcggtag ccactggtgc gtgctggtga gtaaaaccac
      781 gcccacgccg cagcctggca gcgatgaaat caatcgtgct tatgaagaag gatgggtagg
      841 aaatcacgcg ctggcgttta ttggcgacac actttcgcca aagggcgaga aagtgccgga
      901 gctgtttatc gttgagttac cgcaagatga agctggctgg aaagcggcag gtgatgcgcc
      961 gttaagtgga acggaaacca ccctgcccgc gccaccgcgc ggcgtcgtgc agcgacgttt
     1021 aacctttacc caccatcggg cttatccggg gttagtcaac gtcccgcgcc actgggtgcg
     1081 ctgtaatccg cagggtacgc aaatcgcgtt tttaatgcgt gatgataacg gcattgtgca
     1141 actgtggctt atctcgccac agggcggcga gccgcgccag ttaacccata acaaaacgga
     1201 tattcagtct gcatttaact ggcatccgtc aggagaatgg ttgggctttg tgctggataa
     1261 tcgaattgct tgtgcccatg cgcaaagtgg cgaggttgag tatttaaccg aaagccacgc
     1321 caatccacct tctgcggacg ccgtggtctt ctcgccggat ggtcaatggc tggcgtggat
     1381 ggaaggcggc cagctgtgga tcaccgaaac tgatcgctaa atcagttatc aggcattctg
     1441 gccggtggaa taaccgcatt ggtggcttgc gcgttggctt cgcttttttc aacgtgtgag
     1501 cgcaccgagc tgtctttgcg gaacacatcc cacggcagta aaagcgtgtc catcacagcg
     1561 gtaaacggca tatcgagaat cgccagcgac ttagtgcccc agttggtttc atcgtcgcca
     1621 atcatggttg cgctggcgcg ggtgccggga tacgttcctt ctttccctcc ggtatgggac
     1681 atcacgctgg agcatcctcc gagtaatgtc attccgctgc atatcatgaa cgctaacaac
     1741 acatttctta tcataataaa ttcatctgtt gatcgtgggt gttggcctga tgagttatag
     1801 cgatcccttg ctgaaaataa catcatcatt acgtcgcact gtggcggcta tcgcacttta
     1861 acgtttcgtg ctgccccctc agtctatgca atagaccata aactgcaaaa aaaagtccgc
     1921 tgataaggct tgaaaagttc atttccagac ccatttttac atcgtagccg atgaggacgc
     1981 gcctgatggg tgttctggct acctgacctg tccattgtgg aaggtattac attctcgctg
     2041 atttcaggag ctattgatta tgcgtaactt tgatttatcc ccgctttacc gttctgctat
     2101 tggatttgac cgtttgttta accacttaga aaacaaccag agccagagta atggcggcta
     2161 ccctccgtat aacgttgaac tggtagacga aaaccattac cgcattgcta tcgctgtggc
     2221 tggttttgct gagagcgaac tggaaattac cgcccaggat aatctgctgg tggtgaaagg
     2281 tgctcacgcc gacgaacaaa aagagcgcac ctatctgtac cagggcatcg ctgaacgcaa
     2341 ctttgaacgc aaattccagt tagctgagaa cattcatgtt cgtggggcta acctggtaaa
     2401 tggtttgctg tatatcgatc tcgaacgcgt gattccggaa gcgaaaaaac cgcgccgtat
     2461 cgaaatcaac taattcccta aggccgcctg acgcggcctg acatctccat gctcgccgtc
     2521 agggagcata tgcgaatctt cggatttgca ggtacttact cgcttcttag aaggagaaat
     2581 gactatgcgt aacttcgatt tatccccact gatgcgtcaa tggatcggtt ttgacaaact
     2641 ggccaacgca ctgcaaaacg ccggtgaaag ccagagcttc ccgccgtaca acattgagaa
     2701 aagcgacgat aaccactacc gcattaccct tgcgctggca ggtttccgtc aggaagattt
     2761 agagattcaa ctggaaggta cgcgcctgag cgtaaaaggc acgccggagc agccaaaaga
     2821 agagaaaaaa tggctgcatc aagggcttat gaatcagcca tttagcctga gctttacgct
     2881 ggctgaaaat atggaagtct ctggcgcaac cttcgtaaac ggtttactgc atattgattt
     2941 aattcgtaat gagcctgaac ccatcgcagc gcagcgtatc gctatcagcg aacgtcccgc
     3001 gttaaatagc taactagcta ttctctttgc cccgccattc tggtggggct ttttttgtct
     3061 ttcttcttca acaatgcgtt aaatttcgac tgtttaagat atttcggcac gttctttccc
     3121 gtgataatgt gcgctataag gcaaatctgc ttcaccgatt cagccataat ccgtagcaat
     3181 taaaatgtta tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct
     3241 ggctttggtg gcagtcgtcg gtttgtttat cggcaacgtc aaatttcgcg gcataggatt
     3301 aggtattggc ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg
     3361 gatgacatta agtagcgata tgctgcatgt tattcaggaa tttggcctga tcctgttcgt
     3421 ttataccatc gggattcaag tagggccggg cttctttgcc tcattgcgcg tctccggatt
     3481 acgcctcaac ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct
     3541 gcataaactg tttgatattc cactgccggt agtgctgggg attttctccg gtgcggttac
     3601 caatacgcca gcgctggggg cagggcagca gatcttgcgc gacctgggta caccaatgga
     3661 aatggtcgat cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat
     3721 tttgttcacc atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagctca
     3781 gcagcacgag tcttcacgca ccaatggcgg cgcgctgatc aagactatca atattcgcgt
     3841 tgagaaccct aacctgcatg atttagctat taaagatgta ccgattctca acggcgacaa
     3901 aattatctgc tcgcgtctga aacgcgaaga aaccctaaaa gttccttcgc cagataccat
     3961 tatccaactg ggcgatttgc tgcatctggt ggggcagcca gcggatttac ataatgcgca
     4021 actggtgatt ggtcaggagg tcgatacttc gctgtccacg aaaggcactg atttgcgcgt
     4081 cgagcgtgtg gtggtcacca atgaaaacgt gctcggaaaa cgtattcgcg acctgcactt
     4141 taaagaacgc tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc
     4201 cagtggcgat atcagcctgc agttcggcga tatcctcaat ctggtggggc gtccgtccgc
     4261 aattgatgcc gttgccaatg tgctggggaa tgcgcagcaa aaactgcaac aggttcagat
     4321 gctgccagtg tttattggca tcgggcttgg cgtattgtta ggttctattc ccgtctttgt
     4381 gcctgggttc ccggccgcgt tgaaactggg gctggcgggc ggtccgctga ttatggcgtt
     4441 gatcctcggg cgtatcggca gtatcggcaa gctgtactgg tttatgccgc caagtgccaa
     4501 cctcgcgctg cgggagctgg ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg
     4561 tggggatttt gtgaataccc tggtcaatgg cgaagggcta agctggattg gttatggtgc
     4621 cctgatcacc gccgttccgc tgattactgt tggcattctg gcgcggatgt tagccaaaat
     4681 gaattacctg accatgtgcg ggatgctggc aggttccatg accgatcctc cggcgctggc
     4741 gtttgctaat aatcttcatc caaccagtgg tgcgccggcg ctctcttacg ccactgtcta
     4801 tccgttagtg atgtttctgc gaattatcac cccccaatta ctggcggtgc tcttctggag
     4861 tatcggttaa ctcttttccg ggtggagtcg ccgtaaatgg tactccacct ggtattcgct
     4921 ggcatttctg aacatcactg aataattcaa aaactccccg ctctcgctat atgacaacga
     4981 ggtgatccgc agcagtggcg tgtgttccgc taccttcatt tgccgcgcca gcagtctatc
     5041 ggcgagcacc ggcgtcaggc tttcataatt accgccaatc aaaatcccac actcttgttc
     5101 aatatattca aacttcgacc cttccagatg ttgcagcgaa agattacgga acagtttaac
     5161 cggcatatag ctgtcttcca gcatcagcgg tttcccttcc acaaaacgaa cgcgacggga
     5221 gaagtagatc tgctcgttga tttgaatccg taactggctg gcaatggccg gaggcgcagg
     5281 catgatttca aaaatcagca cctggctggt gaccgttttt ccctgccgtt ttaacacctc
     5341 caccagtccg gtcaggctgg cggtttgatg cagcacatct ttgcgcacca ggtaagtgcc
     5401 gctgccgtgg cggcggacca ccagccccca cgctaccagc aggtcaatcg ctttacggat
     5461 ggtcatccgg gatactgcaa attcttccgc caactttttt tcaccgggaa gaaggctgtt
     5521 tagcgtgaaa tccgcggaat taagtcgaat tcttaaccgc tccgcaatgc ttttgtagat
     5581 catctctaga tctctttttt gcctgcaagc ccgggtaatg cgcccacaag ttctgcttaa
     5641 tcgattgaaa aaacatcttt ttttaaagat gtgttcgatg ctgtgtacca gtgctcacag
     5701 atgtctactt tttcgcgaaa acgtagatct ctaccgccca acgaaaagca tgaaagcgat
     5761 cacgaatccc attgggtcgt catgttctgc cgatcgcatc ttcctatcct cgctccaggc
     5821 ctgccgcata accaatcagg cttcctactt acagaattga gaaaagagga tgtggaaatg
     5881 ctcagtcaaa ttcaacgctt tggcggcgcg atgttcacgc cagtgctgct gtttcccttc
     5941 gccgggattg tggtgggtct tgccatcttg ctgcaaaacc cgatgtttgt cggggaatca
     6001 ctgaccgatc cgaacagttt attcgcgcaa atcgtacaca ttattgaaga gggcggttgg
     6061 acggtattcc gtaatatgcc gctgattttt gctgtcggtt tacccattgg ccttgctaag
     6121 caagcgcagg ggcgtgcttg tctggcggtg atggtgagtt tcctgacctg gaactatttc
     6181 atcaacgcga tgggaatgat ctggggaagc tacttcggcg tcgatttcac tcaggacgcg
     6241 gtggcaggta gcggtctgac aatgatggcc gggattaaaa ccctcgatac cagcattatc
     6301 ggcgcaatta tcatttccgg cattgtgacg gcgctgcata accgtctgtt cgataaaaaa
     6361 ctgccggttt ttctcggcat tttccagggg acgtcttatg tggtgattat cgccttcctg
     6421 gtgatgatcc cctgtgcctg gctgacgttg ctcggttggc caaaagtaca aatggggatt
     6481 gaatctctgc aagcgttcct gcgttcggcg ggtgcgcttg gggggtgggt ttacaccttc
     6541 ctcgaacgta ttctgatccc aaccggttta caccacttca tctacggaca gtttatcttt
     6601 ggtccggcag ctgttgaagg cggcattcag atgtactggg cgcagcatct gcaagagttc
     6661 agtctgagcg ccgagccgct gaaatcgttg ttcccggaag gcggttttgc cctgcacggt
     6721 aactcaaaaa tctttggtgc cgtgggcatt tctttagcga tgtacttcac tgccgcaccg
     6781 gaaaatcggg taaaagtggc gggcttgctg attcccgcaa ccttaaccgc catgctggtg
     6841 ggaattaccg aaccgctgga atttaccttc ctgttcattt caccgttgct gtttgcggta
     6901 cacgccttgc tggcggcctc aatgtcgacc gtgatgtatc tctttggtgt ggtgggcaac
     6961 atgggcggag gtctgattga ccaggtttta ccgcaaaact ggatcccgat gttcagcaac
     7021 cacgcggata tgatgctgac ccaaatcgcc attgggttgt gctttaccct gctgtacttc
     7081 gtggttttcc gcacactgat tctgcaattc aacatgtgca cgccgggacg tgaagatgcg
     7141 gaagtgaaac tctactcaaa agccgaatac aaagcctcgc gaggccaaac caccgctgca
     7201 gagccaaaaa aagagctgga tcaggctgcc ggtatcctgc aagccctggg cggggtcggc
     7261 aatatctcca gcattaacaa ttgcgcgacg cgtttacgta ttgcactgca tgacatgtca
     7321 caaacgctgg atgacgaagt ctttaaaaag ctgggagcgc acggcgtctt ccgtagtggc
     7381 gatgccattc aggtgatcat tggtctgcat gtatcccagc tgcgtgaaca gctcgatagc
     7441 ttaattaatt ctcatcaatc agcagaaaat gttgccatta cggaggcagt ataatgacca
     7501 aattctcagt ggttgtcgca ggcggtggaa gcacctttac gccaggcatc gtgttgatgc
     7561 tcctggcgaa tcaggaccgt ttcccgcttc gtgcactgaa attttatgat aacgatggtg
     7621 cgcggcagga agtgattgcc gaagcctgta aagtcatcct taaagagaaa gcgccggaca
     7681 ttgcgtttag ttacaccacc gatcctgaag tggcattcag cgacgttgat tttgtcatgg
     7741 cgcacatccg cgtcggcaaa tacccgatgc gcgaactgga tgaaaaaatc ccgctgcgcc
     7801 acggcgttgt tggtcaggaa acttgcggac ccggcggaat agcgtacggc atgcgttcca
     7861 ttggcggcgt cctggaactg gtggattata tggaaaaata ttcaccaaat gcctggatgc
     7921 tcaactactc caacccggca gcaattgtcg cagaagccac gcgtcgtctg cgcccgaatg
     7981 cgaaaatcct caacatctgt gacatgccaa tcggtattga aagccggatg gcgcaaattg
     8041 ttgggctgca agatcgcaaa cagatgcgcg tgcgctacta cggcctgaac cactttggct
     8101 ggtggacatc aattgaagat ttgcagggca acgacctgat gccccagctg cggcaatatg
     8161 tctctaagca cggttatgtt ccaccgcagc aagatacaca tactgaagcg agctggaacg
     8221 acacctatgc aaaagcgcgg gatgtccagg cactggaccc ggatacattg ccgaacacct
     8281 atctgaaata ttatctcttc ccggattatg ttgttcagca ttccaaccct gaacataccc
     8341 gcgcgaatga ggtgaatgga acatcgcgag aaacaggttt tcgatgcttg ccgcgccatt
     8401 acagcggcag gaaattcagc ggcggccaag ctggagattg acgaacatgc gtcatacatc
     8461 gtcgatctgg cggcggcaat tgccttcaac actcaggagc ggatgttgct gattgtgcct
     8521 aacaacgggg caatccataa ctttgatgat gaagcgatgg tcgagatccc gtgtctggtc
     8581 gggcacaacg gaccagaacc actggtggtc ggcgatatcc cgcagtttca gaaagggtta
     8641 atgagtcagc aagtggcggt ggaaaaactg gtcgtggacg cctgggaaca gcgttcatat
     8701 cagcacctgt ggcaggcgat tacgttatcg aaaacggtac cgagcgcctc ggtcgccaaa
     8761 gctattctgg atgagttgct ggaggccaac aaagcgtact ggccagagtt acgttaattg
     8821 tgtggactgg catccgttgg gtgctggtcc gtattcttta gcacggctga cggcgcataa
     8881 ccaaatttat ccggtgcgcc gtaaaacccc gtccttcagg gcggggatat aaggcgcggt
     8941 tttccaccta accaggtgtt tgctgttgtt caatgtattg gcggatgacg gatattggcg
     9001 cacctccgca gctattggca aaatagccag gactccacaa aacgcctttg tagtaatacc
     9061 tgactgcaat atctggtcga tctcgtcgca gtaacctacc cgatacgcct ttgaggctgt
     9121 ttaccagact ggatatcgcc agtttgggag gatagttaat taacaaatgg acgtgatctg
     9181 gttcgccatc catttaaacc agttcagctt caaaatcagc acatacattt gaaaagtaag
     9241 tgcgtagttt ttctgtcgcg tcatagtcaa aaatctgacg tcggtatttg gtgacaaaga
     9301 ccaggtgaac atgcatcagg aaaacacaat gccttccacg ccggatatcg gtttcttttt
     9361 tcacagacca aagtatcatt tggcctgtga aacgactaca agcatttaaa ttccagttaa
     9421 gacccggtgg tcaacaggag cgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt
     9481 tcaatcgtgc tctggcacgt cagaatgaga atcatgaggc cggtaataaa tacatccctt
     9541 acgggaaaat ggcttcctgg ctggttgagt ggaaaaatgc cactgaaacg caatggctta
     9601 aagatgcccc gtcacagcca ttgcaacagt cactgaaaga ccttgagcgg gcttacaaaa
     9661 acttcttcca gaatcgggct gcttttcccc gattcaaaaa gcggggacag aatgatgtat
     9721 tccgctaccc gcagggcgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac
     9781 tgggctggat gcgctaccgg aacag
//
LOCUS       DAJVTY010000154         9767 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVTY010000154 DAJVTY010000000
VERSION     DAJVTY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.154"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            302..1348
                     /locus_tag="OQQ66_003367"
     CDS             302..1348
                     /locus_tag="OQQ66_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1906171.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1345..2136)
                     /gene="nei"
                     /locus_tag="OQQ66_003368"
     CDS             complement(1345..2136)
                     /gene="nei"
                     /locus_tag="OQQ66_003368"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1906172.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2172..2906)
                     /gene="pxpA"
                     /locus_tag="OQQ66_003369"
     CDS             complement(2172..2906)
                     /gene="pxpA"
                     /locus_tag="OQQ66_003369"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1906173.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2896..3828)
                     /gene="pxpC"
                     /locus_tag="OQQ66_003370"
     CDS             complement(2896..3828)
                     /gene="pxpC"
                     /locus_tag="OQQ66_003370"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS1906174.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3822..4478)
                     /gene="pxpB"
                     /locus_tag="OQQ66_003371"
     CDS             complement(3822..4478)
                     /gene="pxpB"
                     /locus_tag="OQQ66_003371"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1906175.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4501..5244)
                     /gene="ybgI"
                     /locus_tag="OQQ66_003372"
     CDS             complement(4501..5244)
                     /gene="ybgI"
                     /locus_tag="OQQ66_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1906176.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5515..6996
                     /gene="dtpD"
                     /locus_tag="OQQ66_003373"
     CDS             5515..6996
                     /gene="dtpD"
                     /locus_tag="OQQ66_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1906177.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGSFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGTIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7038..8456)
                     /gene="phrB"
                     /locus_tag="OQQ66_003374"
     CDS             complement(7038..8456)
                     /gene="phrB"
                     /locus_tag="OQQ66_003374"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1906178.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8453..8962)
                     /locus_tag="OQQ66_003375"
     CDS             complement(8453..8962)
                     /locus_tag="OQQ66_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1906179.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9247..9621)
                     /locus_tag="OQQ66_003376"
     CDS             complement(9247..9621)
                     /locus_tag="OQQ66_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906180.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
BASE COUNT         2249 a         2675 c         2364 g         2479 t
ORIGIN      
        1 ttcaataaat atgctgaata cgccgatttc accagcgaca tgttggtcta cgaaaaaacc
       61 tatacagctg aactttcgtc cattgcaggt acccccatcg aggctgggcc cttcgataca
      121 gtggtgcttt ttaaaattaa ctataactga tttgccatga tgccctttgc agccggaaag
      181 ggcatctctt tcagaaaatt gctgattttc ctcttttcct tgattactgt tattttttcc
      241 tggcccggct ttctttatgc tgtatcgata caccaaagag aaaataatga gggagcgaag
      301 gatgccagtt ttgcagtggg gaatgttatg tgtgctatca cttctccttt ctattggttt
      361 cctcgcggta catctcccgg cggcgctatt acttgggccg atgatcgccg ggatcatctt
      421 cagtatgcgc ggaattactc tgcaacttcc ccgctccgct tttctcgccg cgcaggccat
      481 tcttggctgc atgattgcgc aaaacctcac tggttcgatt ctcaccacgc tggccgccaa
      541 ctggccgatc gtgcttgcta ttttactggt gacgctgctt tccagcgcca tcgtgggttg
      601 gttattggtg cgctatagct cactgcctgg aaataccggt gcctggggtt cttctccagg
      661 cggcgcagcg gcaatggtcg ctatggcgca agattacggc gcagatattc gcctggtggc
      721 gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg tcacctgcat
      781 gatgctgggt gataacgctg aagcggttaa ccagcaaatc gtctggttcc cgccagtgag
      841 cattaatctc ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct
      901 gcgtcttcct tccggcacga tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg
      961 tcagctcatc accatcgaac taccggaatg gctgctggcg atggcgtata tggcaattgg
     1021 ctggcggatt ggtcttggtt tcgataaaca aatcttactg cgggcactac gcccactacc
     1081 gcaaatcctg ctgtcgattt ttgctctgct ggctatttgt gcaggtatgg cgtggggact
     1141 gacccggttt atgcatattg attttatgac tgcctacctc gccaccagtc ccgacgggct
     1201 tgataccgtg gcggtcatcg ccgcagggag caatgccgat atggcgctta ttatggcgat
     1261 gcaaaccctg cgcctgttca gtattttgct gacggggcca gccattgcgc ggtttatttc
     1321 aacctatgcg ccgaagcggt cggcctagtg ctggcagcct ggacaccagt aaaacgggcg
     1381 cgaggacaac gtggttttct caatgatgcc gccacagcgt tcgcacagtt cgccatcacg
     1441 atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta ttctcatcca cctgcccccg
     1501 cgtagcgtaa gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat ccagttgcgc
     1561 cgcattgaga tctttcgctt tatgatttcc agtcaaccca acctgccaga ggatctccac
     1621 ccgcaaataa ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc ctgcaaactg
     1681 acggttacga aagcgcggtg acaataaccg ttcctttacc acctccggcg tcagattcgg
     1741 atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt gttccggcct
     1801 caacatctca atatccgagg cgctataaag cagaatggtt ttgtcagccg tttgcagttt
     1861 tacccgcaat actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc gccagacgcc
     1921 gtaaagctga ttatggctgt agagcgttaa gtcgttggaa aaatgagtta acaacgcctt
     1981 accacgcgtt cccacatggg taacgtgttg accgataagt tgtgattgat aagtttttaa
     2041 ctgcgggaag gcaaaccaga catcagttag tggtttgcct ttgatcgccg cctccaggtt
     2101 atccgctgca cggcggatct ccgggccttc aggcatggtg atatcctttt ttcattcttt
     2161 attaatcggg gttatgctgc gacaacaatc tccttttcgg caaatgtaga gcgtagtcgg
     2221 cgggcgaacg ccagtgcgtg ctcgccgtcg ccatgcaggc agactgtttg cgccgtgacc
     2281 gttgcccatt cgccggtgat acttttgact ctgccgtgtt gcaccatctc tagcgtttgc
     2341 gccagcgcct gttcttcgtt ttcaatcaac gcgcctggct gacttcgcgg caccagcgag
     2401 ccgtcagcct gataaccgcg atcggcaaac acttcctcgc gcgttgtcag gccatattgc
     2461 ttgcctgcac gaatcagctc gcttcctgcc agcccaacga gaaccaatgc tggatcgcaa
     2521 gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac
     2581 aacatgccgt gcggtttgac atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc
     2641 agcgcgccaa tttgatacag cgtctgggcg taaacggttt ctggcggcag ctgcatggcg
     2701 ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc gacaccattt
     2761 tttattgctt cacgcacgca agcctgcatg gtttgggcat cgcctgcatg aaatccacaa
     2821 gcaatattgg cagaggaaac cagcgttaat agctctgcgt cgctggcgca gccttcgccc
     2881 agatcggcgt tcaggtcaat tttcattgtg tagccgccac gctaattgtt cgaaataacg
     2941 ttgttgatct tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg
     3001 ctgaccgagc ggaatttgcg ccagatggta catatcagcc tcaatgatac aggcaatacg
     3061 cgggtaacca ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc cgttatgcgg
     3121 cacctgcacc acacccggta acaaaccgtg agataacagt tcgcgatcgg tggtgcgttt
     3181 taaaatttgc ccctgtaagc gatagcccat gcggttactt tgcgggctaa gctgccaggg
     3241 cgaacgccag aatgcatcct gcgaggcgcg atcgaactca tgatattccg gccccggcaa
     3301 ggcgcgaatg cggttgcccc acagcagctg tttaacgccc tgcgcttcca taaaatcacg
     3361 cttcgatttg ccaatcggga gtcggtcacc atccttcagt aaacggcctt ccagcccgcc
     3421 aatccccacg ttgagatcgg tgctgcatga ccccattacc ggcggaacat caataccacc
     3481 cgcgaccgcc agataactgc gcatgccgtg ctgcgggcgt ttaagcgtta aacgctggcc
     3541 tgctttcatc ggcaagcgcc agccggtcca gacggcgtta tcatccagcc gcgcttcgca
     3601 accggcaccc gtcagagcaa accacccatc agtttcgaac tcaaccgtta actgaccgag
     3661 cgtgatctcc agcgcggggg cattggcgtc attacccacc agcaggttag caatgcgtaa
     3721 cgcaggcata tccagtgcgc cgcagtggct gatacccgac tggcgaaaac cgtgacgacc
     3781 gccatcctgc acagtggtat acatgcccgc acgaataatc ttcagcatac tccctccttc
     3841 tgcggtacaa agcgcacgct gtctcccgga cgtaataaga tgggttcgtc acgcgccgga
     3901 tcaaacaggc tgagtgaggt atgaccaatc aactgccagc cacccggcgt tgccagcgga
     3961 taaacaccag tctgcggccc gccgatcccg acagaacctg ccggaacgag caagcgcggt
     4021 tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg gaagcccggt
     4081 tgaaaaccta aaaaccagac cacgtattcc acggaggagt gcaattcaac aacctgtttt
     4141 tcgctcaacc cgcaatgcgc cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa
     4201 accaccggaa tttcaataaa gcgagactca ggttccaggg cctcgctctc ctcccaccag
     4261 cgttgcaaaa gctctatggc atccagcgcc agcgactcag gattacgcaa aatcaccgtg
     4321 atattgttca tgccgggaat ggcttcaacc acattcggca tatccaccag acgctgcgcc
     4381 agtcgccaga tccgtttctg gctagccagc gtcaccggcg gttccagttc cagcactacc
     4441 gccgtttcac ctatcagata acaacgcgct cgttgcactt tcgatccctc tgattattcg
     4501 ttatgcagga ttaggaatat caataaaggt cacatcaaga tcggtatttt cattcagcca
     4561 ctcgctcaat gcgcgaatac caccacgttc agtggcatgg tgacctgcag cataaaaatg
     4621 caatccctgc tcgcgggctg aatgaatggt ctgttcagaa acttcgccag taataaaagc
     4681 atccacgcca aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc
     4741 gacgcgctgg accacctcag gtccggtatc gccacaccat aacggcttac gtcccagacg
     4801 cgcttcaatc caggaggcta gttccagtcc cggcacaggc atggtcagct ctccccacgg
     4861 caccaacggc tcaatttcgc ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt
     4921 attgcccagc tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt tgatatcatt
     4981 cgccagcaac gtttttaaac ggttacgctt cattccgcga atgaccggag actctccttt
     5041 ccagaagtag ccatgatgca caatgaccgc atccgcgccc agacgcactg cctcatcgag
     5101 caaagcctgg ctggcggtga caccggtaac aattttttgc accgtctctt tgccttccac
     5161 ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt cgttaatcag
     5221 ttgttccagt tcggtgtttt tcatcttcgc gccttccgta ttctgatagc tacaccgtta
     5281 acatagcgtt acctcgcctg ttcgtcgata gccaatcttt tgacatatta agaaacaaac
     5341 ccgacgccaa aagacgcaaa ttgtttatat catcacgctt cagctaatgt atatttatgc
     5401 aatcagacag catattgcat aaataatcag atctgtatca aagttttaac tttaaggaaa
     5461 cacaagcacg ccgcgcaact attacgtgac gttgccgtca attgtggaat tattatgaat
     5521 aaacacgcat cacagccgcg cgctatttac tatgtcgttg cgctgcaaat ctgggaatat
     5581 tttagctttt acggcatgcg tgccctgctg attctctatc tcaccaatca actaaaatac
     5641 aacgatactc acgcctacga gttatttagc gcctactgtt cgctggtgta tgtcacgcca
     5701 atcctcggtg gctttttggc ggataaagtt ctcggcaatc gcatggcggt gatgctgggg
     5761 gcgttattga tggcgatcgg tcatgtggtg ttgggtgcca gtgagatcca tccgtcattc
     5821 ctctatctgt ccctggcgat aattgtttgt ggctatggtc tgtttaaatc caatgtcagt
     5881 tgcctgctcg gcgagctgta tgagccaacc gatccgcgtc gtgatggcag tttctcgctg
     5941 atgtatgcag cgggtaacgt ggggtctatt atcgcaccta ttgcctgtgg ctacgcccag
     6001 gaagagtaca gctgggcgat gggctttggc ctggcggcgg tgggtatgat cgcgggtctg
     6061 gtcattttct tatgtggcaa tcgtcatttc acacataccc gcggcgttaa taaaaaagta
     6121 ctgcgtgcga caaactttct cctgcctaac tggggatggc tgctggttct gctggtggca
     6181 acgcctgcgc tgattaccgt attgttctgg aaagagtggt cggtatacgc cttaattgtc
     6241 gcgactatta ttggcctggg tgtactggca aaaatttatc gcaaagcaga aaaccagaaa
     6301 cagcgtaagg agctggggct gattgtgacg ctcaccttct tcagtatgtt gttctgggcc
     6361 ttcgcacaac agggcggcag ctcgattagc ctttatatcg accgcttcgt taaccgcgat
     6421 atgtttggtt ataccgttcc gactgctatg ttccagtcga ttaatgcctt cgcagttatg
     6481 ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg
     6541 cgcatctggg gaaaatttgc tcttggtctc ggcctgatga gcgccgggtt ctgcattctg
     6601 accttaagcg cccgctggtc cgcaatgtat ggtcactctt ctctgccact gatggtatta
     6661 ggcctggcgg tgatgggctt tgcggaactg tttatcgacc cggttgccat gtcgcaaatt
     6721 acgcgcattg aaatccccgg tgtgaccggc gtattaaccg gcatttatat gctgctttcc
     6781 ggcacgattg cgaactatct cgcgggcgtg attgccgatc agacatcgca ggcttcgttt
     6841 gatgcttccg gggcgatcaa ctactccatc aatgcatata ttgaagtatt tgatcaaatt
     6901 acctggggcg cactggcgtg tgtaggactg gtactgatga tttggctgta tcaggcgctg
     6961 aaattcagaa accgcgcgct ggcgctggag tcataatagg ccagcaccat acctgataaa
     7021 acgctcgctg cgttttatca ggctcctttc ctcgcttctt cgtacgctgc cagcgttcgc
     7081 agccttgcct ctttgtgatc aactatcggt tgcggataat ccagcatcac acctgctttc
     7141 tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac
     7201 catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgggttgt cgggttgaaa
     7261 atacgaaaat acggcgctgc atcggttccg gttgaagcgg cccactgcca gccaccgtta
     7321 ttggctgcca aatcaccatc aatcagctgc gacatgaaat atcgctcgcc ttcgcgccag
     7381 tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc
     7441 cagccagtgc tgttaagctg acgcatggca gcatcgacaa tcgggtatcc cgttttgcct
     7501 tcctgccagg cctgtaaatg tgcgggattg ctctgccact gcacacgatc cgtccaggca
     7561 ataaacggac aatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg
     7621 cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt
     7681 tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc caggctggcg
     7741 gacaaacgac tggtgccttc cactgccgga aaatctcgtt gttgctcata ttctccggca
     7801 ccgttctggc aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc
     7861 gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgaggg ctctatcgat
     7921 ccgctactac gaacttttgg cgcagcgacg cactccggca tcccttcccg cagccgtttc
     7981 agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattacc ggtcatcacc
     8041 gcgccaggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct
     8101 ctttcaactt ccacatcccg cgcccgctca ttcacttcat actgatagtt ataaaacagg
     8161 tgggtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca
     8221 tccacttcac ggaacaataa aggaatacct ttttccgcaa gcgctatttg tagcccattc
     8281 agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtcgc ccactggcgt
     8341 ggtgtagcga tatacaacgc cagcacgcgt gcagacgaat tgctgcaggc ggcagccagt
     8401 gcgagattat cgtgcaaacg taaatcctgg cgaaaccaga ccagatgggt agtcataaaa
     8461 ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa
     8521 gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg
     8581 gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat
     8641 caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac attggtgtga tcgcgcgggt
     8701 tggcggggtg agaaagcaaa acaactacgc gttggcggta ctggttgtaa tagtcatcca
     8761 gattttgcca ctggtgaata tcagccacga acgggccaag tttgcggtac tccggctgag
     8821 aatgcgccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta
     8881 agccatgttc tttcagtcga tgtaactcat gaagggcaaa gatacggatt agggtgctgt
     8941 catcaaatcg ttgtagattc attactgctc ctgaacggta taaaggagta agtgtagacg
     9001 atggtaaatg aattttatgg ggcagagttt ttgcggggaa gtattttgtt ggtaaaaata
     9061 ttatcaactt catctgagcg agaattattc tactatttcc cgagctgcat tcattttttg
     9121 tattttttat ttttagaaaa taacagttct caaagcttaa ttacctcact attaattgta
     9181 tttcaaacta acaatcgcac tccattttct ctctttaaaa aacataaaca ttgttaaaac
     9241 aaatatttat ttcgttatct taaatgctat ttctacatca ctcgtcctta taatcaaatt
     9301 attcttattc atagctattt ttgcaggttt aaaatagcgg ctttcaccaa agtcagccat
     9361 gaaaattaga ctacaagttc tcccagcctt tgcacatgag aaaaaccacc catcaactct
     9421 atttattcct gaacctttaa caccataata aatcagaatt tctgggtatt gttcatcctt
     9481 cacgttttca tatttttggt atttaattga aaaccaagag tctatactgc caacatcttc
     9541 aaaaaatatt ggagctgatg tttccgcatg aacatatgca gaaaatgcta acaaaatggc
     9601 aatacttagt ttggtaaaaa tatcgttcca cacttttcag gataatctat tggggggaag
     9661 ggatcttttc ctctaaatct aattgctccc actccttgtt tgtcagacca ttcgccattt
     9721 gcatcctgac gataccagtg ataatcccct cccatgccta taaaatt
//
LOCUS       DAJVTY010000155         9493 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVTY010000155 DAJVTY010000000
VERSION     DAJVTY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.155"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..1060)
                     /locus_tag="OQQ66_003377"
                     /pseudo
     CDS             complement(1..1060)
                     /locus_tag="OQQ66_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            2176..2277
                     /locus_tag="OQQ66_003378"
     CDS             2176..2277
                     /locus_tag="OQQ66_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906181.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2641..2904
                     /locus_tag="OQQ66_003379"
     CDS             2641..2904
                     /locus_tag="OQQ66_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCS1906182.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            2904..3044
                     /gene="ykgO"
                     /locus_tag="OQQ66_003380"
     CDS             2904..3044
                     /gene="ykgO"
                     /locus_tag="OQQ66_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCS1906183.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(3079..3306)
                     /locus_tag="OQQ66_003381"
                     /pseudo
     CDS             complement(3079..3306)
                     /locus_tag="OQQ66_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4129..4671
                     /gene="ecpR"
                     /locus_tag="OQQ66_003382"
                     /gene_synonym="matA"
     CDS             4129..4671
                     /gene="ecpR"
                     /locus_tag="OQQ66_003382"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCS1906184.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            4697..5332
                     /gene="ecpA"
                     /locus_tag="OQQ66_003383"
                     /gene_synonym="matB"
     CDS             4697..5332
                     /gene="ecpA"
                     /locus_tag="OQQ66_003383"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCS1906185.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            5390..6058
                     /gene="ecpB"
                     /locus_tag="OQQ66_003384"
                     /gene_synonym="yagY"
     CDS             5390..6058
                     /gene="ecpB"
                     /locus_tag="OQQ66_003384"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCS1906186.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            6084..8603
                     /gene="ecpC"
                     /locus_tag="OQQ66_003385"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             6084..8603
                     /gene="ecpC"
                     /locus_tag="OQQ66_003385"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            8593..>9493
                     /gene="ecpD"
                     /locus_tag="OQQ66_003386"
     CDS             8593..>9493
                     /gene="ecpD"
                     /locus_tag="OQQ66_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCS1906187.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSL"
BASE COUNT         2583 a         2330 c         2221 g         2359 t
ORIGIN      
        1 gaacgatgtt gttattacgc tcaaccaggt catagcggct gcctgccagc attcgacgct
       61 cgcgaatgct gtctgtatcg agttgtttct ccagaggttc gccaatccga taattaactt
      121 ccaggccaaa gcgagtgtca ttctcaccac tcttgccctg cttatgcccg gcactcaggg
      181 tcagaagagg cacaggcgtg taattcactt cagcggaaat cgcatgtgga tctttctggc
      241 gtttatcttt gccaaacagc ccaacttcat cgccataata ctgctcatac atcaggcttg
      301 cgccaagctg cggtcaggcg ggtaaatagc cctcagcacg aatatcccag ccattcgccg
      361 ggcgttcctg ataatcctca atatccggcg attttttcca gccagaagcc cgaatataac
      421 cattggcgct cagtttcaga taatcgcgcc agtattccgc accaacacca atgcgggtat
      481 gactacggga taaatcatgg tcgataaagg tgttcacccc ccccattcag tcatttcctg
      541 aaaaatgacg ccagccaaaa ccaatatttg actgagtacg atcgtctgta cgatgtattg
      601 ccccctgagt gaacaacata tttgtcggcg tatcataaat cggataaagc atttccagcg
      661 aagaatcctt cagcgagaaa tctttatcga cattcagttt gacgcgcgct gtaccatatt
      721 tcccgagcca ctcctgtatt tcctggttag ctttagcggt ggccattccg gtaataaaat
      781 tacgtgtcgc atcgctatct ggctgactgc ttaaaaatgt cccggcattt gcggcaaacg
      841 acgcgacatt tttctccacg ttattatcag cagttaccgt agtatttccc atgctcaacc
      901 gtggctgaac cgcatgctgc gcacgtgccg ccattactgg ggtaaaggtg acagcgagtg
      961 gaaaaagaac ctgaacagag atatttgccc acgccacgca gcgggccaga actgaataac
     1021 gaaatcgtgg ttgtttatga cctgttttat aacgtgacat tgagataatt tcctgatgaa
     1081 cgaattaacg aacataggca gacacaaata atggcgtctg cttgtcagaa aaaccgggga
     1141 tttcccggtt tccttttccg cagaaaagga aataccttta tgtaagaaag tgcaccgcca
     1201 cctgatatat ccagtgtctg tagccactca tcaggtggtt gggcgcggga ttatattttt
     1261 atttatgggt aaaaatttag ctttttgtgc aagtcattaa atagatacct aagttatatt
     1321 tcttactctg cggacagtga ataattcatt attcagactg aataataatg acctgccttt
     1381 aattagcaac tattggaaat acgtcgaagt aggtatatac gccaggataa accgatatat
     1441 ttattatgga tattcatttt ccttcatcgg actgtaaatt gcggggatat cgaacttaca
     1501 ctactcatcg caacactcaa acagatttat gaatttcgtt cataacccct gacaaatcct
     1561 gccgtctaat cgagcttttc cctttacgtt atgcttctca tgaacgagaa cacaacttat
     1621 tcatgaatta accattccgg ataaactatg gaatcaaatt caggcatcga aaggaactgg
     1681 ctggtttccg ttaatagatt aaatatctat tactctcccg agagtaaaac tatcaaatca
     1741 atgagtaacc gatacagcct cacaaattct ttcagataat taacccgtca ctgcggcacg
     1801 ttctctcgta aggcgtgcct gcagtgtaat ccagtatgta tccggcatag ttgacggttt
     1861 aatagaactc aattcctctc cctggtcacc aacaacgata tcttgtataa caccccagga
     1921 gatacgacat tatgatgaga tgcgatatgc tcctcagcac caatcaacgc caggactggg
     1981 ccagtcttga gatcagactc attcccggga ctgacttcag tcgacatata tcaatgacgt
     2041 tggtgcaata aaagatagcc gatgtaatac atctattcaa atttgtgata catgtcaaaa
     2101 aactggttga ccaaactcgt tatttatata agggcactta cgaagtgcgc tcttttttaa
     2161 agcgaggaag taccaatgaa agagaataaa gtacaacaaa tcagtcataa actaattaat
     2221 atcgttgttt ttgtcgcaat tgtagaatac gcctatttat ttctccattt ctattaataa
     2281 cggcaataaa ctgttcactt cagtgatatt taaaatatgc attctctccc tttttgtaag
     2341 taattattat atccgtggga gaggaataca cgctgtcagg taatcaatca tactgcgata
     2401 aatcatcggc cagtaaagtg gagataacct ccattgtcga aaaatccata ctctcagcga
     2461 aaccatcatc aatcactcat ccaggcgttt atgggagcgt cgccaacggc tgttaacaat
     2521 gccagacttc cccgttgcgg aaattccaca taccactaat ggtcacaggg attgggtgtt
     2581 gaaatgatcc ggatgagcat gtatctttat ggttatgcta taacataaca ggtaaaaatg
     2641 atgaagccca atatccatcc tgagtatcgt actgtggtgt tccacgacac cagtgttgat
     2701 gagtacttta aaatcggctc gactatcaaa acagaccgtg agattgagct ggatggcgta
     2761 acgtatccgt acgtgacaat tgatgtctct tctaaatcgc acccgttcta tacagggaag
     2821 ctgagaacag tggcatcaga aggaaatgtt gcacgattca cccaacgttt tggtcgtttt
     2881 gttagcacga aaaaggggtc gtgatgaaag ttcttaactc tctgcgtacc gcaaaagaac
     2941 gccatccaga ctgtcagatt gtgaagcgaa aaggacggct atatgtgatt tgtaaatcta
     3001 atccacgttt taaggccgtt caggggcgta agaaaaaacg ttgattcaaa attcgacgga
     3061 ttaacgatat ttgtctgatt aataatcaga tcggattaat gttggtgtgt ttataacacc
     3121 aacattaatt ttcctgggga tatattcttc ctgttcattt gaggccaact gcctgacgtt
     3181 tctctacgaa tattccatta tcttaatgtt gacttgttga ccagcttcgc ccctgtatgc
     3241 tggcatcaac tctcttttag actgaacacg ccactcagtc tcctcccttt gcggcgcagc
     3301 ctgcattttc actcaaactg ttaagatgat aaatgtggta aatctgttgg tactaacata
     3361 aaaacgttta cgccacagga acagtctgat ccaccggtaa ccccgtcgcc gacgttcgag
     3421 tgccagttag agtaacgcgc acagataact gcactcagtg ccctgacaaa gaggccatcg
     3481 ttcctgtgac agctggcagc cttcgtttaa cttcacttaa tctggctctt gagggcttac
     3541 cgaacagatg acgtacatac gcccgttcaa ttttccatca cttattgtaa tgaacaccta
     3601 taaccatttt gtgcggcatg ttaatccatt aaaatttctt actgattggc aaatcatctt
     3661 caatgacagc tcattatagt tttatattct atcccttacc cttaaaactt gtttttttac
     3721 tagtccatca cacagcgcat taagactatt cctaacactt cagggcaaag ttcctgacca
     3781 atataaaatg caagtaagaa ttgaacgtta tattgccaat aaccttatga aaccaaatgt
     3841 ctttttcttc ttatcaaaaa agcaatattt tcagtttttc taaatattga cttaaccatt
     3901 gaattccttt tccgttcaca tattgacact catcgaaaaa aaaacataaa tttaagccca
     3961 atcgaaaata attaaactta atctcgttta acctttattg atatgtacta cgtatcttat
     4021 ttacttccgg tttactaagg aaactgaatg cacctgtaaa aattacaggt ttggaaagta
     4081 gtgacatggc aaagtgatta cagtagggac tatgaggtta aaaaccatat ggaatgtcaa
     4141 aaccgttctg ataaatacat ctggtctccc catgacgcct acttctataa aggactatct
     4201 gaactgattg tggatatcga cagattaatt tatctgtcgt tggagaaaat tagaaaagat
     4261 ttcgtgttta tcaatctcaa tacggattct ttatctgaat ttataaaccg tgataatgaa
     4321 tggttatccg cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc
     4381 ttagcaaatt attggtacta caacagcaat attaggggcg tggtatacgc tggactgagt
     4441 cgtgatatta gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt
     4501 aagaaagata aaatcacgga ccgggaaatg gaaattatcc gcatgacggc ccagggaatg
     4561 caacctaaat cgattgccag aattgaaaat tgtagtgtga agacagtgta tacccatcgg
     4621 cgtaatgctg aggccaagct gtactcaaaa atatataagt tggttcagta aactccaggc
     4681 aagttagttt taaaaaatga ctcactggga catcacgtcc tcaattcaac tcgggaagaa
     4741 atgcaatgaa aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg gcatgggtgt
     4801 ggcgcaggct gctgacgtaa cagctcaggc tgtagcgacc tggtcggcaa cagccaaaaa
     4861 agacaccacc agtaatctgg ttgtgacgtc actcggtagc ttggcgttcc agtatgccga
     4921 aggcattaaa ggttttaact cacagaaagg tctatttgac gtggctatcg agggtgactc
     4981 aacggctacc gcctttaaac tgacctcacg tcttatcacc aacacattaa ctcagttgga
     5041 tacctcaggt tccacactga atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac
     5101 tggcgatacc gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc ttagcccgct
     5161 ggctaacggt tacaatgcca gcaatcgtac caccgcacag gatggtttca ccttcaccat
     5221 catcagcggc accaccaatg gtaccaccgc agtaaccgat tacagcactc taccggaagg
     5281 catctggagc ggcgacgtta gcgtacagtt cgacgcgacc tggaccagtt aatctctctg
     5341 atgtaccagc aggggtagcc cccctgcttt attcctggac ggaattgtta tgaaaaagca
     5401 ccttctgcct ctcgctctgc tgttttccgg aatatctccg gcccaggcgc tggatgtcgg
     5461 cgatatatca tcgtttatga acagtgacag cagcacgctg agcaaaacga tcaaaaacag
     5521 taccgacagt ggtcgcctta tcaatatccg tctcgaacgg ctctcttcac cgcttgacga
     5581 cgggcaggtt atctcaatgg acaagccgga tgagttgcta ctcactcccg ccagcttgct
     5641 gctacccgcc caagccagcg aagtgatccg cttcttctat aagggacccg cagatgaaaa
     5701 agagcgctac taccgcattg tctggtttga tcaggccctc agtgatgcgc agcgcgataa
     5761 tgccaaccgc agcgctgtgg ccactgcttc cgcccgcatc ggcaccattc tggtcgtcgc
     5821 ccctcgtcag gcgaactacc actttcagta cgccaacggc tccctgacaa atacaggaaa
     5881 tgcgacgctg cggatcctcg cctacggccc ctgcctgaaa gccgccaacg gtaaggagtg
     5941 taaagagaat tactacctga tgccgggcaa gtcgcgtcgt tttacccgcg tggacactgc
     6001 ggataacaaa ggacgggttg cactttggca gggtgataag ttcattcccg tgaaatagat
     6061 agctgtgcag atggataacg acaatgcctt tacgacggtt ctccccagga ctgaaagccc
     6121 agtttgcctt cggcatggtc tttttgttcg ttcagcccga tgccagcgct gctgacataa
     6181 gtgcgcagca aataggtggg gtgattattc cgcaggcctt cagtcaggcg cttcaggacg
     6241 gcatgagcgt cccgctctat attcatctcg ccggtagtca gggtcgccag gacgatcagc
     6301 gaatcggcag cgcttttatc tggctggatg atggacagct acgcatccgg aaaatacagc
     6361 tggaagagag tgaagataac gccagtgtca gcgaacaaac tcgacagcag ctgatggctc
     6421 tggcgaacgc cccgttcaat gaggccctta ccatcccctg actgacaacg cgcagttgga
     6481 tctcagcttg cgccaactgc tgcagctggt ggtcaagcgc gaagcgctgg gcaccgtact
     6541 acgctcacgt agcgaagaca tcgggcagtc cagtgttaac accctcagca gtaatctgag
     6601 ctataacttg ggcgtctata acaaccagtt gcgtaacggc gggagcaaca catccagcta
     6661 tctgtcgctg aataacgtta ctgcgctgcg cgaacatcat gtggtgctcg acggctcgct
     6721 gtacgggatc ggtagcggtc aacaggacag tgaattatat aaagcgatgt atgaacgcga
     6781 ttttgccggt caccgatttg ccggtggaat gctcgacacc tggaacttgc agtcattagg
     6841 gccgatgacc gccatttcag cagggaagat ttacggcctt tcctggggaa accaggccag
     6901 ctccaccatc ttcgacagca gccagtcagc cacgccagtg atcgcctttt taccggcggc
     6961 gggcgaagta catctcaccc gtgatgggcg gctactaagc gttcagaact tcactatggg
     7021 caatcatgaa gtggataccc ggggtctacc gtacggtatt tacgatgtgg aagttgaggt
     7081 gatcgttaac ggtcgcgtga tcagcaaacg cactcaacgg gtcaataagc tgtttagccg
     7141 gggcgcggcg tcggtgcacc actggcgtgg caggtatggg gcggtagctt tcatatggat
     7201 cgctggtcgg aaaacgggaa aaagacgcga ccagctaaag agagttggct agcaggtgcc
     7261 tcgacctccg gctcattgag tacgcttagc tgggcggcaa cgggatatgg atacgataat
     7321 caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg gggcgatcaa cgttaacctg
     7381 caaaatatgc tggccagtga cagctcatgg agcagcatcg gcagcatcag cgctactcta
     7441 ccgggaggct ttagttcgct gtgggttaac caggaaaaaa cccgtattgg caatcaattg
     7501 cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa ctcactgtgg
     7561 tcgaagctgg gcacattcag catcagctac aatgatgacc gccgttacaa cagccattat
     7621 tacacggcag attactatca aaatgtctac agcggtacct ttggttcgct tggcctgcgg
     7681 gccggtattc agcgctataa caacggcgac agcaacgcca atacagggaa atatatcgct
     7741 ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgaccca tcaaaacggc
     7801 tacaccatgg caaacctgtc agcacgcaaa cagtttgatg aagggaccat tcgcactgtt
     7861 ggtgccaatc tgtcacgtgc catctccggc gataccggtg atgacaaaac cctcagcggt
     7921 ggggcgtatg cacagttcga cgctcgttac gccagcggaa cgctgaacgt caatagcgcg
     7981 gcggacggct acgtcaatac caacttgacc gccaatggca gcgtcggctg gcagggtaaa
     8041 aacattgctg ccagcggcgg actgatggca acgctggggt gatattcaac accgggctgg
     8101 aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccgctt aacggcaagc
     8161 gtaactatct cccgctctct ccctatggaa gatatgaggt ggagttacag aacagcaaaa
     8221 actcactcga cagttacgat atcgtcagcg gccgcaaaag tcgtctgact ctctatccag
     8281 gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc ggtcgtatcc
     8341 gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc ggccgaaccc
     8401 gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatacccc actatcgatt
     8461 ttcgctacag tggcaataaa acctgcgaag tggctctgga actcaaccag gcgcgcggtg
     8521 ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga
     8581 caggagaaga gaatgagagt taacctacta ataacgatga taattttcgc gctaatctgg
     8641 ccagtaactg cgctcagagc ggcagtgagc aaaacaacct gggcggatgc accggcacgc
     8701 gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc tggcggggcg
     8761 ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacac tggttcagga
     8821 accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg cctttatacc
     8881 aactggaagt ttgatatgtg gctggaaaat tcgccggttt catctccttt aactggctta
     8941 cgctgcatca actggtacgc agggtgtaat atgaccacca gtcttatcct gccgcaaacc
     9001 accgacgcca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac
     9061 ggcatgttgt cagacgcgtt ttaccagtat atgcaacaaa tgcccgtcgg cagcagcttt
     9121 acaatgacca tcaatgcctg tcagacctct gtgaactatg acgccagcag cggcgcgcgt
     9181 tgtaaggatc aggcctccgg caactggtat gttcgcaatg tcacccatac gaaagcagca
     9241 aatcttcggt taataaatac ccactcgctg gcggaggtat ttatcaacag cgacggggtg
     9301 ccgaccctgg gcgaagggaa cgccgactgc cggacacaga ccatcggcag cctttcagga
     9361 ttaagttgta agatggtcaa ctataccctg caaacaaacg gactcagcaa cacctcaatc
     9421 catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg
     9481 cagttcagtc tga
//
LOCUS       DAJVTY010000156         9492 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVTY010000156 DAJVTY010000000
VERSION     DAJVTY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.156"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..192
                     /locus_tag="OQQ66_003387"
     CDS             <1..192
                     /locus_tag="OQQ66_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1906188.1"
                     /translation="AAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFN
                     PLATLPSGDKVAAAVGITP"
     gene            complement(297..833)
                     /gene="rcdA"
                     /locus_tag="OQQ66_003388"
     CDS             complement(297..833)
                     /gene="rcdA"
                     /locus_tag="OQQ66_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1906189.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            917..2125
                     /locus_tag="OQQ66_003389"
     CDS             917..2125
                     /locus_tag="OQQ66_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906190.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2125..2940
                     /gene="ybjI"
                     /locus_tag="OQQ66_003390"
     CDS             2125..2940
                     /gene="ybjI"
                     /locus_tag="OQQ66_003390"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1906191.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGNGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3026..3310
                     /locus_tag="OQQ66_003391"
     CDS             3026..3310
                     /locus_tag="OQQ66_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906192.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3351..4581)
                     /gene="mdfA"
                     /locus_tag="OQQ66_003392"
                     /pseudo
     CDS             complement(3351..4581)
                     /gene="mdfA"
                     /locus_tag="OQQ66_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4866..5462
                     /gene="ybjG"
                     /locus_tag="OQQ66_003393"
     CDS             4866..5462
                     /gene="ybjG"
                     /locus_tag="OQQ66_003393"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1906193.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5520..6278
                     /gene="deoR"
                     /locus_tag="OQQ66_003394"
     CDS             5520..6278
                     /gene="deoR"
                     /locus_tag="OQQ66_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1906194.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6325..7516)
                     /gene="dacC"
                     /locus_tag="OQQ66_003395"
                     /pseudo
     CDS             complement(6325..7516)
                     /gene="dacC"
                     /locus_tag="OQQ66_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            7763..8388
                     /gene="gstB"
                     /locus_tag="OQQ66_003396"
                     /pseudo
     CDS             7763..8388
                     /gene="gstB"
                     /locus_tag="OQQ66_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8385..>9492)
                     /gene="yliI"
                     /locus_tag="OQQ66_003397"
     CDS             complement(8385..>9492)
                     /gene="yliI"
                     /locus_tag="OQQ66_003397"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS1906195.1"
                     /translation="QSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPDNH
                     GMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLSYS
                     EVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIA
                     LGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAM
                     NPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQ
                     PVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTD
                     RGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2294 a         2454 c         2341 g         2403 t
ORIGIN      
        1 gcggcgcact tcatgatgtc gggtggaaac attctggcct tacaaaaaat tctcggacac
       61 cacgatataa aaatgactat gcgttacgca catctggcac cggatcatct ggaaacggcg
      121 ctccgtttca atcctctggc aacgctgcca agtggcgaca aagtggcggc agcggttggc
      181 attaccccgt aataaccacc actgaccacc aacctaactt attgttttta atctaactta
      241 ttgttttcat taacccgttt acataaatgg gttttttgtt atctaaaatt tatctactac
      301 cctgcaaccc tctcaaccat cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg
      361 acaaaatgca gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt tccgggttca
      421 aaccattgtt cgagcgtttg ctgactgcgc tgcatccagt tttgcattac cgtttttaat
      481 agcggttttc ggctagccag cgcgtagagc tggtacatca gctccatatt atccggcgtt
      541 gcaacctgtg agctgtagat catatcggtg atagcctggc atgcgccttg agcatcacta
      601 acatcgctaa aaaatgcctg atattgccgg gacataatct cagtaaaact gctgaacgcc
      661 tccagcaaca actcatcaat tcctgaaaaa tagtaggtca tcgatcccaa cggtaccccg
      721 gcaagggtag caattttgcg gtgcgtaaca gcatgtattc cgtaaagttt caccgcctcc
      781 agcgtggcct ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt
      841 cctctgtctg caattgtgta caaatgtaca caaccttgct aatgttgtct tccctcttct
      901 tattttgacg tgatctatga ccgtaaattc ttcacgtaat gcattgaaac gccgaacctg
      961 ggcgctgttt atgttcttct ttttgccagg cctgttaatg gcgtcctggg caacccgtac
     1021 gcctgctatc cgcgatattc tctctgtctc gatcgctgaa atgggcggtg ttctctttgg
     1081 tctgtcgatc ggttcgatga gcggtattct ctgctcggcg tggttagtga aacgctttgg
     1141 gacacgtaat gtcatcctgg tcacgatgtc ctgcgcattg atcgggatga tgatattaag
     1201 tctggcactc tggctgacat cgcccctgct ctttgccgtt ggtctcggcg tctttggggc
     1261 aagttttggt tctgcggaag tggcgataaa cgttgaaggt gccgccgttg agcgagaaat
     1321 gaataaaacg gttttgccga tgatgcacgg tttttatagc ctgggcacgc tggcaggcgc
     1381 tggtgtcggg atggcactga cggcctttgg cgttccggca acggtgcaca ttttattggc
     1441 ggcgctggta ggtatcgcgc ctatttatat cgccattcag gcaatccctg acggtacggg
     1501 caaaaatgct gccgatggca cccagcatgg cgaaaaaggc attccttttt atcgcgatat
     1561 ccagttgctg ttgattggtg ttgtagtgct ggcgatggcc tttgccgaag gttctgccaa
     1621 cgactggtta cccttattaa tggttgacgg tcacggtttt agccctactt ccggctcgct
     1681 gatttatgcc ggttttaccc tggggatgac cgttggacgc ttcactggcg gttggttcat
     1741 cgaccgttac agtcgcgttg ccgtggttcg ggccagtgca ctaatggggg cgttgggtat
     1801 tgggctgatt atttttgtcg atagcgcctg ggtcgctggg gtgtctgttg tactctgggg
     1861 actgggtgcc tcgctgggct tcccgctgac catttctgcc gccagcgata ccggccccga
     1921 tgcaccaacc cgcgtcagtg tggtagcaac gaccggttat ctggctttcc tcgtcgggcc
     1981 gccgctgctg ggctatctcg gcgaacatta tggattacgt agtgcaatgc tggttgtact
     2041 ggcgctggtt attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac
     2101 gcagacggcg atggagaata gttgatgagc attaaattaa ttgcggtaga catggatggt
     2161 actttcttaa gcgatcaaaa aacctataac cgtgagcggt ttatggctca gtatcagcaa
     2221 atgaaagcac aaggcattcg ctttgtggtc gccagcggga atcaatatta tcagttgatc
     2281 tctttcttcc ctgaaattgc taatgaaatt gcctttgtgg ctgaaaacgg cggctgggta
     2341 gtgagtgaag gcaaagatgt ttttaatggc gagctgtcga aggatgcctt tactactgtc
     2401 gtggaacatt tgctgacgcg cccggaagtg gaaattattg cctgcggaaa aaatagcgcc
     2461 tatacgctca aaaaatatga cgatgccatg aaaacggtgg cggaaatgta ttatcaccgt
     2521 ctggaatacg ttgataactt tgacaactta gaagatattt tctttaagtt tggcctgaat
     2581 ctttccgatg aactgattcc acaagtacaa aaagcattac atgaggccat cggcgatatt
     2641 atggtgccag tccacaccgg caacggcagc atcgatctga ttatccccgg cgtacataaa
     2701 gccaatggcc ttcgccaact gcagaaatta tggggaatag acgacagcga agtggtggtc
     2761 tttggcaatg gcggtaacga tattgagatg ctgcgtcagg caggctttag ttttgcaatg
     2821 gaaaatgccg gcagcgcggt cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt
     2881 gaaggcgtac tggatgtgat cgataaagtg cttaaacacg aagcgccatt tgaccaataa
     2941 aaacggcagc acgcccggca acacaatacc tgaatgaagg ctgcataatt cgcggaaatt
     3001 aacccgctgc attatttcag gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct
     3061 tttaatgggc tttactggtg tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg
     3121 atacaaagtg gttgtagttc ctgattcagt cagcgttcca caagcggtca gcgtcgcgac
     3181 tgtgccgcaa accgtctatg tggctcctgc gccagctccg gcttaccgcc cgcatcctta
     3241 tgctcgccat cttgccagcg taggtgaagg gatggttatc gaacaccaga ttgacgacca
     3301 tcaccattaa ttcgagaatt cctgatcgca caaagcagtc aggcattttt ttacccttcg
     3361 tgagaatttc ccatctgttt atcttttaaa aagataacca tcagcgacag ccacaaaatt
     3421 ccgttgacaa ggttgaagag attaaactgt ccgttgcccc cgttcagcca ggcatgtttg
     3481 ctgatttcaa taccaacggt aaagatcagc atttgcagca ttcccatcgc cgcagaaacc
     3541 gtacctttac tcatatcgct ggcaaacagg gttaatcgca ccagtcccgc attcgccaga
     3601 ccaataccga aagcataaat acttaacccg gcagtcatcc ataaatacgc gtgcgatgag
     3661 ataaccgttg ccgcagcagc gaccaacaga ccaatcataa tcggccagcc gcccataatt
     3721 atcagcgaac gtacggtgcg gcgcgaggtc agacgcgcta acagcaagtt acccgcaatt
     3781 aacgccccga aaataggcac ttgcagcaag ccatattcat agctgctcaa ctgctcgccg
     3841 gtaatgatga taatcggcga ctgggcgatc cacgccagca atggcaggct aacgaatccc
     3901 agcgccagcg cccccgccac aaagcggccg ttcttcagca ccagcttata gtcacgaccg
     3961 agttctttca gtgacagttt ctcgcctata cgcgtggcgg tttcaggcat agctcgttgc
     4021 agaccgaaaa aggagatcgc tgccaatgcg gcaaacaaaa caaacatccc ctcccagggc
     4081 agcacatgga tccacgccgc gcccaccagc ggaccaagta gcggagcaat cagcgccacg
     4141 ttcgccatca gcgcggtgat cttgatacaa accgcctctt cgaaagattc ctgaattgcg
     4201 gcgtatccca cagcgccaat gaaacagagg cttatgccct gcaagaagcg caacagagtg
     4261 aattgttcaa tgttttgcgc cagtaatatt gccagacagg tgatgataaa ccacaccacc
     4321 cccgccagca tcaccggacg gcgaccaata cgatccgaca gcggccccag gagccattgt
     4381 aaaaacatcc cgcccgccag atatgcggtc attgaagtag gaacccaatc aatgcccgcc
     4441 tgatattgtt ccaccacggc caacataccg ggttgaatca tatctgccga tataggttga
     4501 aaattcgtaa agcaccagac agagagggaa aagtaacgcc tgacgtccaa gcctggcacc
     4561 ggaagctaat ttattttgca tgcaatttct tcgccaataa taatcgcgca gagtttaata
     4621 aaagcgcagc taacgagaaa gcgaattttg tagctgaaac cacggttaag cacattctta
     4681 cattattgcg agtatagcta cgctttcttt aagttttatt taacctatgc ccgttacaat
     4741 cacccaccgt aaacaggccg cttgagggaa ataagacgat gccgctttac ccagtttaac
     4801 ctgcacttta ttctcaacga cttgcctgta ttggctccct tttaatcact ttgcgtcggg
     4861 aagttatgct ggaaaatttg aatctctctc tattctctct tattaacgcg acgccagact
     4921 cggctccgtg gatgatctcg ttggcgattt ttattgctaa agatttgatt accgtggtgc
     4981 cgctgctggc cgcggtgctt tggttgtggg ggcttacagc acaacggcaa ctggtgataa
     5041 aaatcgctat cgcgctggcg gtcagcctgt ttgtgtcctg gacgatggga catctttttc
     5101 cgcacgaccg accctttgtc gaaaatatcg gctataactt cctgcatcat gcggcggatg
     5161 actcattccc aagcgaccac ggtacggtga ttttcacctt tgcactggca tttttatgct
     5221 ggcatcgcct gtggtccggc tcacttttaa tggtgctggc cgtcgtcatt gcctggtcgc
     5281 gcgtttatct tggcgtccac tggccgctgg atatgctcgg tggattgctg gcaggtatga
     5341 ttggctgcct tagtgcccag attatctggc aagcgatggg gcataaactc tatcaacgtc
     5401 tgcaatcgtg gtatcgcttc tgttttgcat taccgatccg caaaggctgg gtgcgtgact
     5461 gatctctgct aaaaagtgta gtattgagcg gctcgcttca ataactattc agagggatta
     5521 tggaaacacg tcgcgaagag cgtatcgggc agctgctgca agaattaaaa cgcagcgata
     5581 agttacatct taaagacgcc gccgccctgc ttggggtttc ggagatgacg attcgtcgcg
     5641 atctgaacaa ccacagtgcg cccgtcgttt tgctcggcgg ctatattgtt ctggaaccgc
     5701 atagtgccag ccattacctg ttaagcgatc aaaaatcccg cctggtggaa gaaaaacgcc
     5761 gggcggcaaa actggctgcg acactggtag aacccgatca gaccctcttt tttgactgtg
     5821 gcaccaccac gccgtggatt attgaagcga ttgataatga aatccctttt accgccgttt
     5881 gttattcgct aaataccttt ctggcgctga aagagaaacc ccattgccgc gcatttctgt
     5941 gcggtggtga atttcacgcc agcaacgcca ttttcaaacc catcgatttt cagcaaacgt
     6001 tgaataattt ttgcccggat atcgcttttt attccgcggc gggcgtgcat gtcagtaaag
     6061 gcgctacctg ttttaatctt gaagagttgc cggtaaaaca ctgggccatg tcgatggcgc
     6121 aaaagcatgt gctggttgtc gaccacagta aatttggcaa ggtccgtccg gcgcgcatgg
     6181 gtgacctgaa acgctttgat attgtggtga gtgattgttg cccggaagat gagtatgtga
     6241 agtacgcgca gacgcaccgt attaagttga tgtattaatg acgtataacc ggatgacgtt
     6301 tcgcgccatc cggttatcag aagattaaga gaaccagctg ccgaaccact gatggaattt
     6361 catcatcacg aaatcccaca tcctaccaaa gaatccgccc tcttccacat tttccatcac
     6421 gatcagcgga cgctgctcaa tggatttacc gttaagctgg aaatcaatgg tcccgacaac
     6481 ctgacctttt ttcagcggtg cggtaagctg cggttccgtt aacgtgtagc tcgccttcag
     6541 gtttttcagc tgcccacgcg gtatggtcac tgagcccgct tcgcctgccc cgagattcac
     6601 ttcgctctta tcaccaaacc agacgcgctg agtcacaaag gtggcatcag gtttaattgg
     6661 cgtcacggtt tcaaagaagc ggaaacccca ggtcaataat ttctcagact catcggtcag
     6721 ttttcgcccc caacactacg gagattaaac gcatatcgcc ctgggtagcc gaagcaacca
     6781 gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc atcaacattc agattgctgc
     6841 tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac tctttctctt
     6901 tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc aatgccatat
     6961 cgcgcgcggt actgaactgc cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt
     7021 tggtcagacc cagttttttg gcataaccat tcatcagacc aataaatgac tcctggctcc
     7081 cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata atcacatctt
     7141 tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc gatgaaccac
     7201 gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata tcggtgagtt
     7261 taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc
     7321 tcgcgggatc cagtttctca tccgcattgc cttctgccag cactttaccg ctggcgtaat
     7381 ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt tcggccgcga
     7441 atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca cgaaggagag
     7501 aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac gttatatcgc
     7561 cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc gtaagttggt
     7621 aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt catttttttg
     7681 ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca gcctgtggct
     7741 gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt caaccaacgt
     7801 aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc
     7861 cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg
     7921 ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc
     7981 tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg
     8041 gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgccca tcgagggatc
     8101 ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt
     8161 tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg
     8221 ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg
     8281 tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc
     8341 actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta
     8401 actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca
     8461 gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact
     8521 ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat
     8581 ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc
     8641 gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc
     8701 ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc gtagtttttg
     8761 cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt cagccacagt
     8821 gcattactcc acggattcat cgccattcct tgcggattac gaatgccata agaccagatc
     8881 tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc gccctgatcg
     8941 gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg
     9001 ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg cccgccaaaa
     9061 tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc ggtcactttt
     9121 gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc atcatcgcca
     9181 acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc taaaaccacg
     9241 tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat
     9301 aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg
     9361 tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg cagtacttcg
     9421 acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg
     9481 aaaaaggatt gt
//
LOCUS       DAJVTY010000157         9442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVTY010000157 DAJVTY010000000
VERSION     DAJVTY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.157"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(267..1610)
                     /gene="gdhA"
                     /locus_tag="OQQ66_003398"
     CDS             complement(267..1610)
                     /gene="gdhA"
                     /locus_tag="OQQ66_003398"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1906196.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1846..2118
                     /locus_tag="OQQ66_003399"
     CDS             1846..2118
                     /locus_tag="OQQ66_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1906197.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2084..2491)
                     /gene="nudG"
                     /locus_tag="OQQ66_003400"
     CDS             complement(2084..2491)
                     /gene="nudG"
                     /locus_tag="OQQ66_003400"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1906198.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2578..3189
                     /locus_tag="OQQ66_003401"
     CDS             2578..3189
                     /locus_tag="OQQ66_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1906199.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3198..4505)
                     /gene="ynjE"
                     /locus_tag="OQQ66_003402"
     CDS             complement(3198..4505)
                     /gene="ynjE"
                     /locus_tag="OQQ66_003402"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS1906200.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4572..5225)
                     /locus_tag="OQQ66_003403"
     CDS             complement(4572..5225)
                     /locus_tag="OQQ66_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1906201.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5225..6760)
                     /locus_tag="OQQ66_003404"
                     /pseudo
     CDS             complement(5225..6760)
                     /locus_tag="OQQ66_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(6733..7899)
                     /locus_tag="OQQ66_003405"
     CDS             complement(6733..7899)
                     /locus_tag="OQQ66_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1906202.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVNTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7909..8457)
                     /locus_tag="OQQ66_003406"
     CDS             complement(7909..8457)
                     /locus_tag="OQQ66_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1906203.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8457..9164)
                     /locus_tag="OQQ66_003407"
     CDS             complement(8457..9164)
                     /locus_tag="OQQ66_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1906204.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFISTGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9179..9442)
                     /gene="ydjY"
                     /locus_tag="OQQ66_003408"
                     /pseudo
     CDS             complement(9179..9442)
                     /gene="ydjY"
                     /locus_tag="OQQ66_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
BASE COUNT         2259 a         2581 c         2444 g         2158 t
ORIGIN      
        1 catatctgct gctatcgcag aaagttaacg gatgctgaac ttgaagcgtg ttatcgttac
       61 gacaagcagt tttgggagca ctacagtaag aaatgccgtt ggcccataag aaatgttccc
      121 tggggagcat catccgttaa atactcataa acgcctgaaa ttttgccggg ggcgctgcgc
      181 ttgcccggcc tacattagag cgtaactgca atgaattgtg cccatttgta ggcctgataa
      241 gcgtagcgcc atcaggcatt tacaacttaa atcacaccct gcgccagcat cgcatcggca
      301 accttcacaa aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc accttcacca
      361 ccatgctcaa cacaggcatg gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact
      421 ttctcggctt tccagcccag gcgcgcagcg ttttgcgcca tttcaaggcc tgacgttgct
      481 acaccgccag cattagccgc tttacccggt gcaaacagta cgcctgcctg ctggaacagt
      541 tcagtcgctt cgatggtggt cggcatattt gccccttcgg cgacggcttt aacgccattg
      601 gcgataagct gatgcgcggc gccaacatcc agttcgttct gggtggcgca aggcagggcg
      661 atatcaaccg gtacagacca cggctgttgg ccttcgagat agaccagacc aaattctttg
      721 gcgtaatctg ccactcgacc atcgcggctg gctttgattt cgataagacg tgccagtttc
      781 tctttcgtga atccgctttc atcaactaca gtgccgctgg agtctgacgc agtgatcaca
      841 cgagcaccaa attccatcgc tttttcgata gcgtactggg cgacgttgcc ggagccagaa
      901 acggaaacgc gcatcccttc aaaacccata ccgtggcgtt ttaacattgc ttctgtgaaa
      961 taaaccagac cgtagccggt agcttccggg cgaataagac taccgccaaa tgaaaggccc
     1021 ttaccggtga agacgcaggc ggtattgttg gagagctttt tcatcatccc cgccataaag
     1081 ccgacttcac gaccaccaac gccgatatca cctgccggaa cgtcagtatc cgcgcccaga
     1141 tgacgataaa gctcggtcat cagcgcctgg cagaaacgca tcacttcgct ttcgctttta
     1201 cctttcgggt caaaatcgct accgccttta ccaccgccca tcggcagggt agtcagggca
     1261 tttttgaagg tttgttcgaa gccgaggaat ttgagaatgg agaggttaac tgacggatgg
     1321 aagcgcatac cgcctttgta cgggccgatg gctgagctga actgcacacg ccatgcacgg
     1381 ttgacctgta cctggttgcg atcatcaacc cataccacgc gaaactggat cacgcgctcc
     1441 ggttcaacca gacgctccag taatgacatc tggcgatatt ttggattttg ttcaagaaaa
     1501 ggccagagtg tggtcattac ttcacgaacg gcttgcgcga actcggtttg attcgggtcg
     1561 cgcttttgga catggttgag gaatgactcc agagaatatg tctgatccat agatataaaa
     1621 cccttatata ttaatacgat tgtgcttatg ttgtttatgt catgtgcttt tgcagttttc
     1681 gactatacca tcaagaatgt gacggtaagc aagaaaaaaa tgacccagga aagcaaatta
     1741 ataacgagag taatctcata accaaatgcg ataactcaga cgctaaagtt ttgccacccg
     1801 gctaccgtta tcagttgcag agacaataac gttaaggagt tccctgtgag tcgagcattg
     1861 ttcgccgtag tattagcatt tccattaatt gcactggcta acccgcatta tcgtccggac
     1921 gtggaggtaa acgtaccgcc agaagtgttc agttcaggcg ggcagtcagc gcagccctgt
     1981 actcagtgct gcgtttatca ggatcaaaat tattctgaag gggcggtaat taaagccgaa
     2041 ggaatactgc tgcaatgcca gcgggatgac aaaaccctta gcactaatcc gctgctctgg
     2101 cggcgcgtaa agccataaac gcctctaata gtggaatgtc agtaggggcc agcggatatt
     2161 gcagcgcctc ttcaggtgag caccagacca gcgcctgatg ttcatgtgcc tgtaacgtcc
     2221 cgtggaagtc gggtacgtgc caggcatgaa gatggataat ccggcccgaa acttctcgct
     2281 gatggctggc aacatactca cccacagttg cttcgatgcc caattcttcg tttaactcac
     2341 gcaccagtgc ctgctgctgg ctttcatcca gctcgacttt accaccggca aactcccata
     2401 atcccgcctg atcgctctgg gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa
     2461 tgatggcggc aacaacttca atcattttca taaattacac acgggcagat aagcgaaaaa
     2521 tgccataata tccgcactgt tgctcaatca gaaaggcttt atatttaagg agtgcctgtg
     2581 ctagaccgcc atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt gcgggttctt
     2641 gataaaccgg gcattacgcc agatggttta acattagtgg gatttgccat cggcgtgctg
     2701 gctctgccgt ttctggcgct gggctggtat ctggcggcgc tagtcgtcat tttgttgaac
     2761 aggctgcttg atggtctgga cggtgcgctg gcgcggcgca gagagcttac cgacgcgggc
     2821 ggctttcttg atatttctct cgattttctc ttttacgcgc tggtgccgtt tggctttatt
     2881 cttgctgcac cggagcaaaa tgcgctggca ggcggctggt tgttgtttgc gtttatcagc
     2941 acgggcagca gttttctcgc ctttgctgca ctggcagcaa aacatcagat tgataacccc
     3001 ggttatgcac ataagtcgtt ttactatctg gggggattaa ccgaaggcac agaaacgatc
     3061 ttactgtttg tgctgggatg tttattccct gcatggtttg cgtggatctt cggtgcgttg
     3121 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     3181 cgtcagtaat tgcaatgtta tttgctactg tccgggccgc gttcaccggt tgctaccgga
     3241 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     3301 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     3361 gaaacttgct gctctggttt gatattccac gctttccaca tagcggtaat atcatcggcg
     3421 ctgcgcatgg tgccatccgg gttatgaaaa tcttccatat gcgtcgagtc gctaccagcg
     3481 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     3541 gtaccgataa attctggcca tgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     3601 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     3661 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     3721 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     3781 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     3841 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     3901 agcggttcgc tttccacttc gttggtatcg atgtagtcag caccgggaat atggctgata
     3961 aggtaaagct taggagcgcc ccaggccgct tcaatgactt tccagtcacc ggcaggtttc
     4021 gccgtaacct ctttaccttg ttgcaggtcg tgcagccatt gcggataaac cagctgctca
     4081 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     4141 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt cgacatcttt gtcattaccg
     4201 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     4261 tcggtgctca ttttgtcgag ccagctggca gagaggttta aggcggcagg ttcatgacca
     4321 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     4381 gctttgccat tttgttgttg aagctggtca agtgtaagag gcttcgccag ttcagcggcc
     4441 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     4501 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     4561 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     4621 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagt
     4681 tcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     4741 ctgaatggct catccaggag taacgctttt ggctgagcga gaagggcgcg cagcagggcc
     4801 acccgcgctc gctgaccgcc tgataatgtc gcagggtcct ggtgataagt ttcagccagt
     4861 cccgctcgat caagcgcgtc tttcacggca ttgcgcctgg cagtcccctt gagcgtcgac
     4921 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctgg
     4981 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     5041 catagctcac ctgtacaaga aaactgtcca gccagtgcac caatcatcca tgaaaacaga
     5101 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     5161 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     5221 agcattagcg gagtccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     5281 caaaaataat aagcggtaac aacagttgcc ataaagcctg ggcggcgaga ataccgttgc
     5341 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     5401 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     5461 gcacagggcg caacatgagt gtgcatttca cgtaaaagaa gattttggcc cgctaccagc
     5521 ccagtgtttg tgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     5581 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     5641 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     5701 atagaatggg caaccatagc cacaactggc gacgctgtgg cccccattcc agccacagca
     5761 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     5821 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     5881 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     5941 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccatagcaga tagctcagca
     6001 aaacgtaggc ggcgagtaac agcatcagca gcagactggc gagtgcgcct ttcgtttgtt
     6061 gatccgcgtc gccctgagtt aaccactgcc agctaattac tgccagcgtc ggtggattac
     6121 ctggcccaag aataatcgcc acatccacga ccgaaagcga ccaggcaaca atcgccagca
     6181 tcgccattgc cagcgaaggc gcgacggagg gcaacagcag ccagttcagg cattgccagc
     6241 ggctgtagcc cagcgaatcc agcacaatga cctgctgcaa cagccgtttt tcgctcaata
     6301 ctgccgctaa gatccacagc agaaatgcgc tttctttcac tgcaagggtg aggcccagac
     6361 cgatgccaaa tcggtccatt ggcggagtga aatacgggaa atagtcatat agcagccctc
     6421 cgtcagcaaa gagcagcagg gcgctggtgg caaaagccac atggggaatg gcgagcagcc
     6481 acggcagacg ggcgcacata cgctgccatt tcggcccagg ccatagcgcc acaatcacca
     6541 gcagggcaat caacaatgcc ccgaccgccg cgatggttgt cgacaccagc gttgccagta
     6601 atgcatgcgg taactgtgga tcggcaaata acgcctgcca gtgtgtcaac gacaacgcag
     6661 gcgatatcag cgtgagagct gccgggatca gcggtgcata aatcaccgcc accatcgccc
     6721 acagcaaaaa gattaatgcg taccgtaacg gcgtaaccat tcttgttcca gtgcattcac
     6781 ccatcctgcg tgcggttcag ccagtaccgg cggcagatcc tgcggcattc ttgattgcaa
     6841 tatttcgcgc tgcccgtcag gcagtttttg cggatcgaga acagaaggat cgtcccagac
     6901 aacgggatct gctttacgca gttgcgcatc gggtgagagc agaaaattgg caactacctt
     6961 cgccgcagca ctggcattcg cgttggctgg aatggtgacg aaatgcacgt tgccaatcat
     7021 cccctcgcga aagccaaaac tgtaactgct tgccggcaaa tcgccgctgg cgattttttg
     7081 ctgcgcatgc gcggggttaa aggtcagcga caggcgcaat gtgccggctt tcagcagagc
     7141 atccatccgc gcgggcgaag gcgggaaatc ctttccttcg cgccacaaat acggatgcag
     7201 tgcatcaaga tattgccaca agggcgcagt gacacgggcg aaagtcgcat cgtccggcgc
     7261 ttcttttaat gcggcgggat ctggcgtcag cataatcagc aactgttcaa gaaacgccgt
     7321 gccggtaaag tccggtgggc gcggataagt aaccgtgccg ggattggctt tagcaaactc
     7381 cagtaaggct tgcggcgttt gtggtggctg tggcgtaaca tcgcggcggg cgataaacgt
     7441 cagttgtgcg ccgccccagg gcgattccgc accttgtgtc ggcactgaaa aatcttcccg
     7501 caccggcagc tgtgtgttga catagcgcca gttgggcaga gtctccgccc agcccgtttg
     7561 taataaattg gcctctttta aggtgcggaa gttttcgccg ttcacccaga gcagatccac
     7621 cgagccgccc gttttacgac cggcagcagc ttcggtctga atgcgcttca ccgcgtctgc
     7681 ggcatccgcg agacgaacaa tcttcaggtt tatagcgtaa tgggttttca tctcgccgct
     7741 aacccagtcg agatagcggt taattgcggt atcgccgccc caggcgttaa accagacggt
     7801 ttgccctttg gcctcatttt taatttcctg ccagtcactg gcatgtgttg ccaaagaaaa
     7861 cagcgataac aatcccagca accacccaca atggcgcatc ttctctcctt aagaacgttt
     7921 ttccggaata cggcacagcc cctgagcggg aatgtccatg gcagaattaa aacgagccga
     7981 caggttttgc aggccgatca atgccgtcag ttcggtgagc gcctgagcgt caaaatgcgc
     8041 agccagtcgg gtacgcaggg catcatcgac tgttggcggt gtgacgcttg cggcttcggc
     8101 gtactccagc gccagccgtt cttcatcgct aaagagcggg ctttgccgcc agtcagccac
     8161 tgccagcagt ttatcggtgc tgccggtacg ctcggcgact ttcatactgg tgatatccac
     8221 acaaaactca cacaggcaca tttgcgcaat gcgcgcgctg acaagcgatc gcactaccgg
     8281 atcgagcggt gagcgtttgc gctccagcca cccaacaaac attgacacca aataaaagat
     8341 aaacgggatc cgcccccacc agcgaatcgg gcttagcacc tcgccgtaat ggcgacgatg
     8401 cagccacgcc tgcggacgta aaatgaaagg aattttgcta agcggcggta aacccatcag
     8461 tcattcctct tctggcgctc aagccatatt ttcttcgcca taaaaatcac cacggtcagg
     8521 gcaaacagaa taaacagtcc ggtgacaaac cagaaagtac cgcctgttaa catgctgccc
     8581 gcccaggaat aaacaatagt cgccggtaat tgaccaagcc cggtggagat aaaaaacgag
     8641 cgaaaacgta ttgaagtcag accggcagca tagctgattg gatcgaaagg gacaaaaggc
     8701 aataaccgac agaccaggat ggtgtgtttg ccgtagcgag tgaaaaagcc gtccatactg
     8761 tcaagcacgg ttttgccggt taatttttcc accacttcgc ggcccatcac tctggcgata
     8821 aaaaagcaca gcgccgcgcc ggccatcgaa ctggtccacg acagcaagcc gccccagaac
     8881 gcgccaaaca gcgacgcatt ggcaaaggtg atcaaaaacg caggtagcgg tgcagcaatg
     8941 gcctgtaaaa tcatcaacaa gaacgagaca actgccgcca gtgcgccgta agactgaata
     9001 aagcgttcta taccctgttg gtccacggcg gcaaacgccg caacgctgcg attgataaac
     9061 tcatggacgc cgggaagcag cgcccatgcg agcagcatag cgaacaacag gatgatgagg
     9121 gttatacagt agtaccagat tttacgcgat tgcatcttca tcatccttta tcctggcttt
     9181 attcggtgat tttaaaggta accgttgcca gcgtgttatc cgccgggaga actgaggcat
     9241 tgcctttgaa tttcacttcg ccgcgttttt ccaccgcacc ataagtgtat gttgcattgc
     9301 tgacgatgcc gaccgggcag ctatccagac acaccagtca acccgttttc ttctcttctg
     9361 ctgccgttaa attaccgcca aagcgcatgt ccagtttctt gccattactg tcaacaatca
     9421 cttcatcgaa ggaatacgct tt
//
LOCUS       DAJVTY010000158         9384 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVTY010000158 DAJVTY010000000
VERSION     DAJVTY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.158"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..526
                     /locus_tag="OQQ66_003409"
     CDS             <1..526
                     /locus_tag="OQQ66_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1906205.1"
                     /translation="LPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAPPGDI
                     LLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAF
                     TGICNDLAAASACM"
     gene            424..1104
                     /locus_tag="OQQ66_003410"
     CDS             424..1104
                     /locus_tag="OQQ66_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1906206.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            1345..4818
                     /locus_tag="OQQ66_003411"
     CDS             1345..4818
                     /locus_tag="OQQ66_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1906207.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            4886..5485
                     /locus_tag="OQQ66_003412"
     CDS             4886..5485
                     /locus_tag="OQQ66_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1906208.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5550..6957
                     /locus_tag="OQQ66_003413"
                     /pseudo
     CDS             5550..6957
                     /locus_tag="OQQ66_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            7043..7555
                     /locus_tag="OQQ66_003414"
     CDS             7043..7555
                     /locus_tag="OQQ66_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906209.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            7631..7843
                     /locus_tag="OQQ66_003415"
     CDS             7631..7843
                     /locus_tag="OQQ66_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1906210.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(7985..8309)
                     /locus_tag="OQQ66_003416"
                     /pseudo
     CDS             complement(7985..8309)
                     /locus_tag="OQQ66_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(8445..8693)
                     /locus_tag="OQQ66_003417"
     CDS             complement(8445..8693)
                     /locus_tag="OQQ66_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1906211.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(9048..>9384)
                     /locus_tag="OQQ66_003418"
     CDS             complement(9048..>9384)
                     /locus_tag="OQQ66_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1906212.1"
                     /translation="VLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGI
                     DTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVR
                     SVEEILHAL"
BASE COUNT         2317 a         2242 c         2770 g         2055 t
ORIGIN      
        1 gctgccctgg ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg
       61 gctggtctgc cggggggaaa ttcataaatt ccgctgtgtg ccacatctga caggacggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcatacc atctggcggg
      181 aattgatatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc agaacctgta
      241 cctggacaat atggcggtca ccggctttta ccgggtgccc ctgtcctctg caccgccggg
      301 cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccacca tttactgcgg
      361 caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga
      421 aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt
      481 cacggggatt tgcaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa
      541 gccattcgcg ccctgtcgat gcagatgccg ggctttcgcc gtcagatgaa cgaaggctgg
      601 taccagatac gtattgccgg tgatgacacg gcaccggagg cggtgtatgc ccgtcttcac
      661 gaacagctgg gtgagggaac ggtcatccac attgtgccgc gactggccgg ggccggaaag
      721 ggtggactgc agattgtgtt gggggcggca gccatcgtgg gctctttctt cactgccggg
      781 gcatcaatgg cgttatgggg ttcagccctg gcagccggtg gtttttctgc caccacgatg
      841 ctgttttcac ttggagccag catgattctg ggcggtgtgg cccagatgct ggccccgaag
      901 gcaaaaacac cggattaccg cgcaacggat aacggcagac agaacacgta cttttcctcg
      961 ctggataaca tgattgccca ggggaacccg atgccggtgc cttacgggga aatgctggtt
     1021 ggctcccgcc gtatatccca ggacatcagc acccgtgatg aaggcggggg cggaaaggtc
     1081 gtggttatcg ggcgacaggg ataaaacata aaaaaatccc gcagtgatcg cggagctgcg
     1141 gggacagaca aatgaagatc aatgtgaagg agttgttttt gttactcggg caaaaaaaca
     1201 ctaacgcagc gaaattataa gcgccacagt cagtgtgtga aaatgtgaag atattcagaa
     1261 attttattcc gtcatgacgc aggcacccgg tgaggtgcct gttgtttttg tgagtgaaca
     1321 attatcacgg taagaggtga tgtaatgggc aaaggtggcg gcagggcgca cacaccgcgt
     1381 gaggcgaaag acaatctcaa atccacgcag atgatgagcg tgattgatgc gattggtgag
     1441 ggaccgatag aaggcccggt gaaaggcctg cagagtattc tggtgaacaa aaccccgctg
     1501 acggacacgg acggtaatcc cgtgatacac ggtgtgaccg cggtctggcg tgccggggag
     1561 caggagcaga caccaccgga aagctttgag tcctccggct ctgaaactgt actgggtgtc
     1621 gaagtgacca gggcaaaacc ggtaacacgc accattacgt cagcgaacat tgaccgcctg
     1681 cgggtgacct tcggggtgca gtcactggtg gagaccacgt caaagggtga ccgtaatccg
     1741 tcctctgtcc gtctgctgat tcagttacag cgtaacggta actgggtgac agaaaaggat
     1801 gtcaccatta acggcaagac cacctcacag ttccttgcgt cggtgattct ggataatctg
     1861 cctccccgcc cctttaacat ccggatggtc agggagacgg cggacagcac cacggaccag
     1921 ctgcagaaca gaacgctgtg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
     1981 ccgaacacgg ccattgtggg gatgcaggtg gatgcggagc agtttggtgg tcagcagatg
     2041 acggtgaact accatatccg cggtcgcatc atccaggtgc cgtcaaacta tgacccggaa
     2101 aaacgcacgt acagtggtat ctgggacggc agtctgaaac cggcatacag caacaacccg
     2161 gcctggtgcc tgtgggacat gctgactcac ccgcgctacg gcatgggaaa atgtctgggg
     2221 gcggcggatg tggacaagtg ggcgctgtat gccatcgggc agtactgcga ccagacggtc
     2281 ccggatggtt tcggggggac cgagccgcgg atgaccttta atgcgtacct ggcacaacag
     2341 cgtaaggcgt gggacgttct cagtgatttc tgctctgcga tgcgctgtat gccggtatgg
     2401 aacggtcaga cgctgacgtt cgttcaggac cgcccgtcgg atgtggtgtg gccgtacacc
     2461 aacagcgatg tggtggtgga tgataacggc gtgggattcc gctacagctt cagtgccctg
     2521 aaggaccggc acacggcggt ggaggtgaat tacaccgacc cgcagaacgg ctggcagacc
     2581 tccacggaac tggtggaaga cccggaagcc atactgcgct acggacgcaa cctgctgaag
     2641 atggatgcgt ttggctgtac ctgccgcggt caggctcacc gtgcaggtct gtgggtgata
     2701 aagaccggac tgctggaaac gcagacggtg gacttcacgc tcgggtcaca ggggctgcgt
     2761 cacacaccgg gtgacattat tgaaatctgt gataacgact atgctgggac tatgaccggc
     2821 ggacgtgtcc tgtccattga tgctgccacc cgcaccctga cgctggaccg tgaggttacc
     2881 ctgccggaga caggtacatc ggcggtgaac ctgattaacg gcagtggtaa gccggtgagt
     2941 gtggacatca ccgcacaccc cgcgccggac cggatacagg tcagtaccct gcctgatggt
     3001 gtggagacat acggggtgtg gggactctcc ctgccgtcac tgcgccgtcg cctgttccgc
     3061 tgtgtctccg tccgggaaaa cacggatggc acctttgcca tcacggcggt gcagcacgta
     3121 ccggaaaaag aagccatcgt ggataacggt gcccgctttg agccgcagtc aggctccctg
     3181 aacagcgtca tcccaccggc agtgcagcac ctgacggtgg aggtgagcgc agctgacggc
     3241 cagtatctgg cacaggcgaa atgggacacg ccgcgggtgg tgaagggggt gcgcttcagt
     3301 ctgcgactga ccagcggaag cggagaagac agccgtctgg tgaccaccgc tatcactgcg
     3361 gatacagagc atcgtttcag tggtctgccg ctcggggaat acaccctgac agtcagggca
     3421 attaacagtt atggccagca gggcgaaccg gccaccacca ccttccggat taacgcgcca
     3481 gcaaaacccg ccaccattga actgacgccg gggtattttc agataacagc ggtaccgcgt
     3541 cttgcggtgt atgacccgac ggtacagttt gagttctggt tttcggaggc aaaaatcgca
     3601 gacacatctc aggtggaaac ctctgcccgt tatctgggga ccggcagtca gtggagtgta
     3661 tccggcccgc acattaagcc cgggaaggat ttctggtttt acgtgcgcag cgtcaacctg
     3721 gtggggaaat ctgcttttgt ggaggccagt ggacgggcca gcaatgatgc agaagggtat
     3781 ctggggctgt ttcgggaaaa aataggaaaa ctgcatctgg ctcaggggct gtgggagctg
     3841 atagacaaca gccagcttgc ggatgagatg gcggagatga agaccaccat caccgaaacc
     3901 cgcaatgaaa tcacacagac ggtcagtaaa acgctggagg accagagcgc caccatacag
     3961 cagatacagc gcgtgcagaa ggacacaaat gatgaccttg ctgcacttta catgctgaag
     4021 gtacagaaaa caaaaaatgg cataccctat gttgccggta ttggagcggg gattgaggat
     4081 actgatggcc agcccctgag caacatactg ctgctggctg accgtattgc gatgattaac
     4141 ccggaggacg gcaacaccac gccgttattt gtggcgcagg ggaatcagtt gttcatgaac
     4201 gatgtgttcc tgaagcggct gtttgcggtg agtatcacct cgtccggcaa tcccccgacg
     4261 ttttccctga cgccggaggg caggctgacc gcaagaaatg ctgatatcag cggtaacgtg
     4321 aatgcgaact ccgggacgct caacaacgtc acgattaacg agaactgccg ggttctggga
     4381 aaactgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggtaa ggcttttccg
     4441 cgggactccc gggcaccgga gcggtggcca tcagggaccg ttaccgtcag gatttatgac
     4501 gatcagcctt ttgaccggca aattgttatt ccggcggtgg cattcagcgg cgctaagcat
     4561 gagagagagc atactgatat ttactcctca tgccgtctga tagtgcggaa aaacggtgct
     4621 gaaatttata accgtaccgc gctggataat acgctgattt acagttgcgt tattgatatg
     4681 cctgccggtc acggtcacat gacgctggag ttttcggtat cagcatggct ggtgaataac
     4741 tggtatccca cagcaagtat cagcgatttg ctggttgtgg tgatgaagaa agccaccgca
     4801 ggcatcagta tcagctgaat tttataaccc atatacgggc gccagaaatg gcgccttttt
     4861 tattgcagaa aagcgagagg taattatgcg taaagtttgt gcagccattt tgtccgcagc
     4921 catctgtctg gctgtatccg gtgcgcctgc atgggcgtct gaacatcagt ccacactgag
     4981 cgcggggtat cttcatgccc gtacgaacgc tcccggcagc gataatctga acgggattaa
     5041 cgtgaaatac cgttatgagt ttacggacgc gctggggctg attacgtcct tcagttatgc
     5101 caatgctgaa gatgagaaaa aaacgcacta cagcgatacc cgctggcatg aggattccgt
     5161 tcgtaaccgc tggttcagcg taatggcggg gccgtctgtg cgcgtgaatg aatggttcag
     5221 cgcgtatgcg atggcgggtg tggcttacag ccgtgtgtcg actttctccg gggattatct
     5281 ccgcgtaact gacaacaagg ggaaaacgca cgacgtgctg accggaagtg atgacggtcg
     5341 ccacagcaac acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt
     5401 ggccattgat attgcttatg aaggttccgg cagtggcgac tggcgcactg acggtttcat
     5461 cgtgggtgtc ggctataaat tctgattagc caggtaacac agtgtcatga cagcccgccg
     5521 gttcaggcgg gcttttttgt ggggtgaata tgacagtaaa gatttctggt gtactgaaag
     5581 acggcacagg aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagta
     5641 ccacggtggt ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagta
     5701 tggacgttga gtatggtcag tacagcgtta ttctgttggt ggagggattc ccgccgtcac
     5761 atgccgggac tatcaccgtg tatgaagatt ctcaacccgg tacgctgaat gattttctcg
     5821 gtgccatgac tgaggatgat gcccgtccgg aggcactgcg tcgctttgaa ctgatggtgg
     5881 aagaggtggt gcgtaacgca gaggaggcga agaagaatgc cggagaggcg gagacgtcag
     5941 cgaggaatgc cggcatatca gccagtcagg cagaagagag cgctgcaaat gctgacactt
     6001 cagcagggga tgcatcggag tcagcccggc aggcggcaga aagtgcagcc tcagcaaagc
     6061 agtcagagga ggcgtcctcg tcctcggcct ctgcggccgc tcaaaaagcc agtgagtcat
     6121 tacaaagtgc aacagatgct gagttgtcaa aaaagacggc agaaagtgca gccggtaatg
     6181 cagtcaggga tgcaacgacc gcaacagaaa aagcccggga gtcagcagaa agcgcacagt
     6241 cagcggaaca aagcaggata gcggcggaag aggccgtaaa ccgaatcccc accgtggtgg
     6301 gacctcccgg gccaaagggg gaaccggggc ccgcgggtcc tcaggggccg aagggagata
     6361 aaggagagcg tggcgacacc ggcccggcag gggccaaaag gtgacaaagg cgatccggga
     6421 gtggctggac cagcaggtcc ggcaggtgcg ccggggccga aaggcgataa aggtgatccg
     6481 ggagtagcag gtccagcagg tccggcgggg ccacagggac cgaagggaga cacaggagcc
     6541 cccgggcaag gaacagaact gcttactact gccaatacat ggactcaggc acaaactttt
     6601 aatggtggta ttaatggcaa tttgacggtg accggaaacg gctcatttaa cgatattcag
     6661 atccgttcgg ataaacgcaa caagcgaaat ctggtaaaac tggataatgc gttagatcgt
     6721 ctggaggcac ttactggtta tctttacgag atacagtact ctgccgacgg ttggcaaacg
     6781 tcggttggtt taattgctca ggatgcacaa aaagcattgc ctgaactggt aactgaagac
     6841 gcagacgtta tatctggtga aaaacgtctg cgtcttaact acaacggcat aattgcattg
     6901 ttagtcgagg gctttaaaac acttcgtcat gagattaaag aactccggga gaagtaaacg
     6961 acagctgttg tagtttctgg tttctactga atttaaattg tggggtgaca ctcaccccac
     7021 gcattcagaa gggggagatg agatgggggt aacatcggga tgggtaggct cttcggctaa
     7081 gagcgaaaca ggtgagcagt ggatgggagc cgccggaacc aaactgggac tgagtaaacc
     7141 ttttatgatg agccaaatgg ttgggcgaac tatgggctgt aaaattgcaa ctgagtacta
     7201 taaatggaaa tcctctgaca aggttgataa ctggggcgca gttggcgctg actggccttt
     7261 agaagaaaaa agtaaaggta caattacaaa cgccgcaagc tgcggatcag ggaggctggt
     7321 aggggctgtc gttacacttt ctcacttttt gacgaactct acaccgacag ctgctgttta
     7381 tttagccggt ggtaaagcag gtaacatcac cgtaaacgta ggtggtgcta cacaaaccat
     7441 gatttatcag ggcgttgtta gtgggttcca gtattactgg tcaggttctg ttagttccgc
     7501 tttcgtggag gcaatgaaaa agacgggagt accccaggat ctaaaaatta gttaaatggt
     7561 aaataaataa ttttaaaaac ttcacgttct acataccgga gacgccggat atataggata
     7621 ttgttttaag ttgccagaga aatttttccg gacggatgct gataataatg atgtgccaat
     7681 gacggcagca tcatggatgg cactgagtga agcgacagag caggcgatgt ttgcgaaggg
     7741 agtggagatt aatacacggc agttgcagat gaaagccgag gttgaggcgc tgacagacct
     7801 gaaggcgatc cgcagttatg ttgtcggatg gcctgctggc tgaataaaaa aacgggacca
     7861 cgaccagtcc cggaaccatg agttttaggg tattagtttg ttatcatagt tagcgtgcta
     7921 agtatgccat gtcaggttga ttagtgaagt gatgttgttc gcatttttgc acggcggaga
     7981 atattcagat ttttggaaat ccatattttt cccgtgcgcg attacatgct tcatttacga
     8041 tgctattttc gccggacatt gcgaattccc tgcatgtgga cggtttttgt aaacagagca
     8101 atatgcgttt tctccggggg ggccggcaag agcgccacat cggggatttt tctgattggt
     8161 gccgcgcata cagcgatgaa aaggagatat ttgctcagtg agactgaccg aaacctttcc
     8221 gccagcatcg tcagcttctg cccagtaaaa agagacgcgg aaaatgcaca acaggcacca
     8281 cacgtcatgc atggattcag attgctcata attcaccagt acagctataa atcgtaaaga
     8341 gaacagcggt acatcgtatg taagaacgta ccgcggctgg ctggtgaact tccgatagtg
     8401 cgagtattga atgatttcca gccgttaccg attttacgtg ctaattagtg aacaaaccac
     8461 tcgtcagcag actcccaggt atctttcaga gtttcctgaa caaaagtttt agctgaatct
     8521 ttatcggcgg tgcgcgtaac agaaaggcca tcgttgctgg tagcttttac gatcacctct
     8581 acatcgtcat aacgtttact gatgcgtcgg gttaattctt cctttaacgc atccacagca
     8641 ccggttggca ttttagtcat tttttctttg gctatgcaga tttcaatacg cataaaagtc
     8701 cctctacact gtgtttgtat acagtatcat ttttaactgt atggataaac agtattaagg
     8761 gtgaaggaaa gtgatttttt ggggcatgca tggggcaaaa atatttgttg tggggcattt
     8821 tggggcatga ttgggacact ttgttacata tgaactttgc cgatttttat atgacatgga
     8881 aatacaacgc tttgattctt ctaaataaaa aacatgctct tgggcgttct ttagtgattt
     8941 ttaaaaattc cgtatcacgc agttaaagtg gcgggcatac tcttcaaggc tggtgatccc
     9001 caaccgcacc catttaggat gcgaccattg cggtagaccg atgtaaatca taacgcgtga
     9061 aggatctctt ccacgctacg cacacgggcg atgcgcgggt agatatgatt aatgctgtta
     9121 ttgtgttgct cggcgctggc ggcactacag gcgtcttcgg caatcaccag attaaagcca
     9181 agttcccagg cattgcgggc ggtggattca acaccgatat tggtcgagat cccacataac
     9241 actattgtat cgatacctcg gcggcgcagt tgcaactcca gatccgtacc gtaaaacgca
     9301 ccccactgac gtttgatgat ttcgatatca ctgtcagttg cacctaatgc agctggatgt
     9361 tgccaccagt tttcgggcaa cact
//
LOCUS       DAJVTY010000159         9362 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVTY010000159 DAJVTY010000000
VERSION     DAJVTY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.159"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            291..1826
                     /gene="gadC"
                     /locus_tag="OQQ66_003419"
     CDS             291..1826
                     /gene="gadC"
                     /locus_tag="OQQ66_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1906213.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1957..3276
                     /locus_tag="OQQ66_003420"
     CDS             1957..3276
                     /locus_tag="OQQ66_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1906214.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3648..5029
                     /gene="dosC"
                     /locus_tag="OQQ66_003421"
                     /pseudo
     CDS             3648..5029
                     /gene="dosC"
                     /locus_tag="OQQ66_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            5054..7453
                     /gene="dosP"
                     /locus_tag="OQQ66_003422"
                     /gene_synonym="pdeO"
     CDS             5054..7453
                     /gene="dosP"
                     /locus_tag="OQQ66_003422"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS1906215.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7711..8292
                     /gene="ddpX"
                     /locus_tag="OQQ66_003423"
     CDS             7711..8292
                     /gene="ddpX"
                     /locus_tag="OQQ66_003423"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS1906216.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8306..>9362
                     /locus_tag="OQQ66_003424"
     CDS             8306..>9362
                     /locus_tag="OQQ66_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1906217.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVT"
BASE COUNT         2437 a         2290 c         2280 g         2355 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacata cctgataacg tttaacggta acggtgtccc gaaacgaacc cgtttcggga
      181 caatttccaa agtctgttca ctggcattag caacggaaaa tattgttctg aatacgcttc
      241 agaacaaaac aggtgcggtt ccgacaggaa taccgtttta gggggataat atggctacat
      301 cagtacagac aggtaaagct aagcagctca cattactcgg attctttgcc ataacggcat
      361 cgatggtaat ggctgtttat gaatacccta ccttcgcaac atcgggcttt tcattagtct
      421 tcttcctgct attaggcggg attttatggt ttattcccgt gggactttgt gctgcggaaa
      481 tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca aatactctgg
      541 ggccgagatg gggatttgca gcgatctcat ttggctatct gcaaatcgcc attggtttta
      601 ttccgatgct ctatttcgtg ttaggggcac tctcctacat cctgaaatgg ccagcgctga
      661 atgaagaccc cattaccaaa actattgcag cactcatcat tctttgggcg ctggcattaa
      721 cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa agttggcttc ttcgccggta
      781 tcctgttacc tgcatttatt ttgatcgcat tagcggctat ttatctgcac tccggtgccc
      841 ccgttgctat cgaaatggat tcgaagacct tcttccctga cttctctaaa gtgggcacac
      901 tggttgtatt tgttgccttc attttgagtt atatgggcgt agaagcttcc gcaactcacg
      961 tcaatgaaat gagcaacccc gggcgcgact atccactggc tatgttactg ctgatggtgg
     1021 cggcaatctg cttaagctct gttggcggtt tatctattgc gatggtcatt ccgggtaatg
     1081 aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt tctgatgtcc catgtggcac
     1141 cggaaattga gtggacggtt cgcgtgatct ccgcactgct gttgctgggt gttctggcgg
     1201 aaatcgcctc ctggattgtt ggtccttctc gcgggatgta tgtcacagcg cagaaaaacc
     1261 tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt accggtaacg ctggtcattt
     1321 cgcagctggt gattacttct atcgcgttga tcatcctcac caataccggt ggcggtaaca
     1381 acatgtcctt cctgatcgca ctggcgctga cggtggtgat ttatctgtgt gcttatttca
     1441 tgctgtttat tggctacatt gtgttggttc ttaaacatcc tgacttaaaa cgcacattta
     1501 atatccctgg tggtaaaggg gtgaaactgg tcgtggcaat tgtcggtctg ctgacttcaa
     1561 ttatggcgtt tattgtttcc ttcctgccgc cggataacat ccagggtgac tctaccgata
     1621 tgtatgttga attactggtc gttagtttcc tggtggtact tgccctgccc tttattctct
     1681 atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt cactctggag ccaatcaaca
     1741 gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg tgcacgttca ccacactata
     1801 ttgttatgaa tgacaagaaa cactaaccat gtctgaatgg ttataagaca agggagcgat
     1861 aattcatcgc tccctttttc gtgcttgctg tcgtattgac ttaaccggga taagtacgag
     1921 aatgagcgca catctgttta ccggaaacca gcacatatgg atatctgctc ccgaaacaag
     1981 aaattaacga ttagaagacc agcgatactg gttgcactgg cacttttact gtgtagttgt
     2041 aaaagcacgc ctccagagtc catggtgaca ccaccagcag gttcaaagcc accagccacg
     2101 acgcaacaat cgtcacaacc gatgcgtggc atctggctgg ccacggtgtc tcgcctcgac
     2161 tggccaccgg tttcctcggt taacattagt aaccccacca gccgggcccg tgtacaacaa
     2221 caggcgatga tcgacaaact ggatcatctg caacgtctcg gtataaacac ggtctttttc
     2281 caggtcaagc cggacggtac cgccctgtgg ccatcaaaaa ttttgccgtg gtccgatctt
     2341 atgaccggta agattggtga aaatccgggt tacgatccgc tgcaatttat gctcgacgaa
     2401 gcccacaagc gtgggatgaa agtacacgcc tggtttaacc cctatcgcgt atcggttaat
     2461 acgaagcccg gtactatcag ggaactgaat agcactctgt ctcaacaacc ggcgagcgtc
     2521 tatgtgcaac accgtgactg gatcagaacg tctggcgatc gctttgtcct cgacccgggc
     2581 atccctgagg ttcaggactg gatcacatca atagtcgccg aagtggtttc ccgctatccg
     2641 gtagatggcg tgcagtttga cgactatttc tatacggagt caccgggttc acggctaaat
     2701 gataacgaaa cgtaccgtaa atacggaggc gcatttgcgt caaaagcaga ctggcggcgc
     2761 aacaatactc agcagttaat tacaaaagta tcgcacacca ttaaaagcat taagccggga
     2821 gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga tccgctcggt
     2881 tccgataccc gaggcgcggc agcctatgac gaatcctacg ccgacacccg tcgatgggtg
     2941 gaacaaggat tgctggatta cattgctccc caaatttact ggccgttctc acggagtgcc
     3001 gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg ttaaaccgac caggacccgc
     3061 ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt caaagataga gccagactgg
     3121 atgattaacg gcggcgtacc ggaactgaaa aagcagctcg atcttaacga tgcggtacca
     3181 gaaattagcg gcaccatctt gttccgtgag gactatctga ataaaccgca gactcaacaa
     3241 gcggtcagct atctgcaaag tcgttggggc agttaaactc ccatgcttac tttgtaacaa
     3301 gccggtgctt tacccaccgg cttgttacac cttgtgaaat atcccactag taatcatgct
     3361 tacataagtc aaattaacca cgcaattaac cacacttagt taaacagcta taaatcccta
     3421 ataaaaatgt atgaatatga aaaaccatac gatattcgct gtcaccgaaa aataattacg
     3481 atgggctata ctcttctgaa gaacagtagc gtagattgaa actgtcctaa gtcgcttatt
     3541 tcttttcagt atatcttcat atttcaggag aatatggtgc agttagtcga tctcgcacgt
     3601 tgcgttttga ttccgtctga tttcccagcc ttataagggt gtgaattatg gagatgtatt
     3661 ttaaaagaat gaaagatgag tggaccggac ttgtcgaaca ggcagatcca ctcattcgtg
     3721 ctaaagccgc ggaaattgcc gttgcgcatg ctcattatct gagtatcgag ttttatcgaa
     3781 ttgtccgcat cgacccgcat gccgaagaat tcttgagtaa tgaacaagtt gagcggcagt
     3841 tgaaaagtgc gatggaacgc tggattatta acgtgctttc tgcccaggtt gacgatgtcg
     3901 aaaggctaat acaaatccag cataccgtcg cggaagtgca tgcccgcata ggaattccgg
     3961 tagaaattgt cgagatgggg tttcgggtgc tgaaaaaaat cctctatccg gtaatcttct
     4021 cttcggatta ttctgccgca gaaaaacttc aggtctacca tttctcgatt aacagtattg
     4081 atatcgctat ggaagtgatg acccgcgcgt ttacctttag tgacagtagt gcctcaaagg
     4141 aagatgaaaa ctatcgtatc ttctcgttac tagaaaacgc cgaagaagaa aaagaacggc
     4201 aaatagcctc aatactttca tgggaaatag atattatcta taaaatcctg ctggattctg
     4261 atttaggcag tagtttgcct ttaagccagg ctgattttgg cctgtggttt aaccataaag
     4321 gtcgacatta ttttagtggc attgccgaag taggcatatc tcccgtctga ttcaggattt
     4381 cgacggtata ttcaatcaaa ccatgcgtaa caccaggaat ttgaataaca gaagtctgcg
     4441 ggtgaaattt ttattacaga taagaaatac tgtatcgcaa attattacct tgctgcgtga
     4501 attgtttgaa gaagtatcgc gccacgaagt cggtatggat gtactgacga aattacttaa
     4561 ccgccgcttc ctgccgacta tcttcaaacg cgaaattgcc catgccaacc ggaccggtac
     4621 accgctgtca gtgctgatta ttgacgttga taaattcaaa gagatcaacg atacgtgggg
     4681 ccataacact ggcgatgaaa ttctgcgtaa agtctctcag gccttttatg acaacgtccg
     4741 cagtagtgat tatgtttttc gctacggggg cgatgaattt atcattgttt tgactgaagc
     4801 ttctgaaaac gaaacgttac gtaccgcaga acgtattcgc agtcgggtgg agaaaaccaa
     4861 actgaaagcc gcaaacggcg aggatattgc cctctcactt tccatcggtg ccgccatgtt
     4921 taatggtcat cctgactatg agcgcctcat tcaaatagcc gatgaagctc tgtatatcgc
     4981 caaaagacga ggtagaaacc gtgttgaact ctggaaagcc agtctttaga tgcgccagga
     5041 tgcagaggta atcatgaagc taaccgatgc ggaaaatgcc gccgatggca tttttttccc
     5101 cgcccttgaa caaaatatga tgggcgcggt gttaattaac gaaaatgatg aagtgatgtt
     5161 tttcaacccc gccgcagaga agctctgggg gtacaaacgt gaagaagtca ttggcaataa
     5221 cattgatatg ctgattccgc gggatttgcg tcctgcgcat cctgaataca ttcgtcataa
     5281 ccgtgaaggt ggtaaagcgc gcgtcgaggg gatgagtcgg gagctgcagc tggagaaaaa
     5341 agacggcagt aaaatctgga cccgttttgc gctatcgaaa gtgagcgccg aggggaaagt
     5401 ttattacctg gcgctggtac gggatgccag cgtagaaatg gcgcaaaaag aacagacccg
     5461 acaattgatt attgccgttg accatctcga ccgaccggtg attgtcctcg atccggaacg
     5521 ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt ggttactgca ttagcgaagc
     5581 cagcggtatg cagcccgata cactcctgaa cattcctgaa ttccctgccg ataaccgcat
     5641 tcgtttacaa cagttgctat ggaaaaccgc ccgcgatcag gacgaatttc tgctgttgac
     5701 gcgcaccggt gaaaaaatct ggattaaagc ctctatcagc ccggtttatg acgtgctcgc
     5761 gcatctgcag aacctggtaa tgactttctc ggatatcacc gaagaacggc aaatccgcca
     5821 gcttgaaggc aatattctcg ccgccatgtg cagcagcccg ccatttcatg aaatggggga
     5881 aatcatttgt cgtaacatcg aatctgttct caacgaatcg catgtttcgc tgttcgcact
     5941 gcgcaacggg atgccgatac actgggcgtc atcttcgcac ggtgcagaag ttcaaaatgc
     6001 gcaaagctgg tcagcgacca ttcgtcagcg tgatggcgcg cctgcgggga tcctgcaaat
     6061 taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa cgcgtggcag atatcagtca
     6121 gcatatggcc gcgctggcgc tggaacagga aaaaagccgc cagcatattg aacaactcat
     6181 ccaatttgat ccgatgaccg gtctgccaaa tcgcaataac ctgcacaatt acctcgatga
     6241 cctggtcgac aaagccgtct ctccggtggt gtatctcatc ggtgttgacc atattcagga
     6301 tgtgattgat agccttggct atgcgtgggc cgatcaggca ttgctggaag tggtcaatcg
     6361 ctttcgtgga aaactcaaac cggatcagta tctctgtcgt atcgaaggta cgcagtttgt
     6421 cctcgtgagc ctggaaaacg acgtcagtaa cattacccag atcgccgatg agctacggaa
     6481 tgtggtcagc aagccgataa tgattgacga taaaccattc ccgcttacct tgagtattgg
     6541 catcagctac gacgtgggta aaaaccacga ttacctactc tccactgctc acaatgcaat
     6601 agattatatt cgcaagaatg gcggtaacgg ctggcagttc ttcagcccgg cgatgaacga
     6661 aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa gaagcgatta gcaataacca
     6721 actgaaactt gtttaccagc cgcaaatctt cgcagaaacg ggtgaactgt acggcatcga
     6781 agcccttgcg cgctggcacg atccccagca tggtcatgtg cccccttcac ggtttattcc
     6841 tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc tgggtcatcg cggaagcttg
     6901 ccgtcagtta gcagaatggc gtagccagaa tattcatatc ccggcgcttt ccgttaactt
     6961 gtcggcactg cactttcgca gtaatcaact gcctaatcag gtgtctgatg caatgcacgc
     7021 ctggggtatt gacggccacc agctgacggt agaaatcacg gaaagcatga tgatggaaca
     7081 cgataccgaa atctttaagc gcattcagat cctgcgtgat atgggcgtgg gcttatcggt
     7141 agatgatttt ggtacgggct tttccggatt atcccgctta gtcagtcttc cggtaacgga
     7201 aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacc gaaaaacgca tccttgcctt
     7261 acttgaagcc attaccagca ttgggcaaag cctcaattta accgtcgtgg cggaaggcgt
     7321 cgaaaccaaa gagcaatttg agatgctacg caagatccac tgtcgcgtta ttcagggata
     7381 tttcttttcc cgccccctac ccgccgaaga aattccaggc tggatgagca gcgtgttacc
     7441 gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg ttttcattta acgtgacacc
     7501 gtcacttaaa tactgtgatt tcatctacga ttccgggaga ttccttcctt aacttcccct
     7561 cttgcaccaa aactgcacta cgctgcacaa atgttgatca aaagttaaaa aatataactt
     7621 taagccagca ttctggcgct tatcccggca tggcatgaga tctgcataag cggaaagcgc
     7681 agcaattttt gtcttataca ggaaccgttt atgtcggata ccaccgaact ggttgattta
     7741 gccgtaatct tccctgatct ggagatcgaa ttgaaatacg cctgcgctga taacatcaca
     7801 ggtaaagcta tttatcagca agcgcgttgt ctgctacaca aggatgcgat taccgcgctg
     7861 gcgaaaagta tcagtatcgc ccagctgtca ggcttacaac tggtgattta cgatgcgtat
     7921 cgcccacaac aagcacaggc gatgttgtgg caagcctgcc cagacccgca atatgttgtt
     7981 gatgtgacgg tcggttctaa tcacagccgt ggcacggcga tcgacctgac gcttcgtgat
     8041 gagcacggga acatcctcga tatgggcgca gggttcgatg aaatgcacga gcgctcccat
     8101 gcctatcacc cttccgtccc gcccgccgct cagcgcaatc ggctgttgct gaatgcgatt
     8161 atgactggtg gcggttttgt cggtatctcc agcgaatggt ggcacttcga attaccccag
     8221 gcagcgagtt acccactgct cgctgatcaa ttcacctgtt ttatatcccc tggcacacag
     8281 cacgtcagtt aatcaggaaa ctgtcatgaa gagatcgata ccgtttcgtc ctacattgct
     8341 cgcgctcgtc cttgccacaa ctttcccggt tgcgcacgcc gccgtaccga aagatatgct
     8401 ggtgattggt aaggccgccg atccacaaac cctcgccccg gcggtaacaa tagataataa
     8461 cgactggacg gtgacctacc cgtcttatca gcggctggtt cagtacaaaa cggacggtga
     8521 taaaggctca accgacgttg aaggcgatct ggcaagtagc tggaaagcgt ctgacgatca
     8581 aaaagagtgg acgttcaccc tgaaggacaa tgctaaattt gccgatggca caccggtcac
     8641 tgccgaagca gtaaaactct cttttgagcg gctactaaaa atcggccagg ggccagcaga
     8701 agcatttccc aaagatttaa agattgatgc tcccgacgaa catacggtga agtttaccct
     8761 tagccagcca ttcgcaccgt tcctctacac gctggcgaat gacggtgcat ccattatcaa
     8821 tccggcggtg ttaaaggaac atgcagcgga tgatgcccgc ggcttcctcg cgcaaaatac
     8881 cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa ggtcagcaat tagttctggt
     8941 gccaaatccg cattaccctg gcaataaacc gaactttaag cgagtatcgg taaaaattat
     9001 tggtgaaagt gcctcccgtc gcctgcagct ctcccgtggt gatattgaca ttgccgatgc
     9061 gctgccggtg gatcaactca acgccctgaa gcaggaaaac aaagtcaatg tggcagagta
     9121 tccgtcactg cgcgtcacct acctgtatct gaataacagc aaagcgcctc ttaatcaggc
     9181 ggatctgcgt cgggccattt cctggtctac cgattatcag ggcatggtta acggcattct
     9241 gagtggtaac ggaaaacaga tgcgcggccc gattccggaa ggcatgtggg gctacgatgc
     9301 gacggcaatg caatacaacc atgacgaaac gaaagccaaa gccgaatggg ataaagtgac
     9361 ga
//
LOCUS       DAJVTY010000160         8857 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVTY010000160 DAJVTY010000000
VERSION     DAJVTY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.160"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            194..1027
                     /gene="ppsR"
                     /locus_tag="OQQ66_003425"
     CDS             194..1027
                     /gene="ppsR"
                     /locus_tag="OQQ66_003425"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1906218.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1183..2229
                     /gene="aroH"
                     /locus_tag="OQQ66_003426"
     CDS             1183..2229
                     /gene="aroH"
                     /locus_tag="OQQ66_003426"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1906219.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2361..2552
                     /gene="hemP"
                     /locus_tag="OQQ66_003427"
     CDS             2361..2552
                     /gene="hemP"
                     /locus_tag="OQQ66_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1906220.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2556..3992)
                     /locus_tag="OQQ66_003428"
     CDS             complement(2556..3992)
                     /locus_tag="OQQ66_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1906221.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4055..4768)
                     /gene="ydiV"
                     /locus_tag="OQQ66_003429"
     CDS             complement(4055..4768)
                     /gene="ydiV"
                     /locus_tag="OQQ66_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1906222.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(5014..5478)
                     /locus_tag="OQQ66_003430"
     CDS             complement(5014..5478)
                     /locus_tag="OQQ66_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1906223.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5556..6305)
                     /gene="btuD"
                     /locus_tag="OQQ66_003431"
     CDS             complement(5556..6305)
                     /gene="btuD"
                     /locus_tag="OQQ66_003431"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1906224.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVGLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6305..6856)
                     /gene="btuE"
                     /locus_tag="OQQ66_003432"
     CDS             complement(6305..6856)
                     /gene="btuE"
                     /locus_tag="OQQ66_003432"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1906225.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEGPIVMESIKLALAK"
     gene            complement(6919..7899)
                     /gene="btuC"
                     /locus_tag="OQQ66_003433"
     CDS             complement(6919..7899)
                     /gene="btuC"
                     /locus_tag="OQQ66_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1906226.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8108..8437)
                     /locus_tag="OQQ66_003434"
     CDS             complement(8108..8437)
                     /locus_tag="OQQ66_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906227.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
BASE COUNT         2341 a         2349 c         2014 g         2153 t
ORIGIN      
        1 ttgcgcaaac ctgctctatt cgacaatttc cacataaacc atcggtataa acgaaaccat
       61 aaaaatgaaa tgctgttttc ataaaaaata aaattgaagg ttcattttat aaaccagaca
      121 ttacctccta cgcttctgtg cgtttttaat ttatgctttc atagaattat gtctgcatca
      181 cgggaagaac aaaatggata atgctgttga tcgccacgtt ttttatattt ctgatggtac
      241 ggcaataact gcggaggtat taggacacgc agtaatgtca caatttcccg tcactatcag
      301 cagcattacg ctgccgtttg tcgaaaatga gagccgtgca cgggcagtga aggatcagat
      361 tgacgcaatt tatcaccaga caggtgtgcg cccgctggtc ttctactcca tcgtgttgcc
      421 ggagattcgc gccatcatct tgcaaagtga aggcttttgc caggatatcg ttcaggcgct
      481 ggttgccccg ctacaacaag agatgaaact ggatccaacg ccgattgctc atcgtaccca
      541 tggccttaac cctaataatc tcaataaata tgatgcgcgc attgcggcga ttgattacac
      601 ccttgcccac gatgacggca tttcgttgcg caatctggat caggctcagg tgatcctgct
      661 cggtgtttct cgctgtggta aaacccccac cagtctgtat ctggcaatgc aatttggtat
      721 ccgcacggca aactacccct ttattgccga cgatatggat aatctggtgc tacccgcgtc
      781 gctcaaaccg cttcagcata aattgttcgg cctgactatc gacccggaac gtctggcggc
      841 gattcgcgag gaacgtcggg agaacagtcg ctatgcctcg cttcgtcagt gcagaatgga
      901 agtcgcggaa gtggaagcct tgtaccgtaa aaatcagatc ccgtggatta acagtaccaa
      961 ttattcggta gaagagattg ccaccaagat cctcgatatc atgggcctta gtcgccgaat
     1021 gtactagaga actagtgcgc tggtttgttt tttgttatca tgctaaccac ccggcgaggt
     1081 gtgacacacc tcgcacttga aatcagctgc gattggttta tcgtgatgcg catcacttcc
     1141 cggtagtcct gccgtagaag caacaaattt ctgagacttg taatgaacag aactgacgaa
     1201 ctccgtactg cgcgtattga gagcctggta acgcccgccg aactcgcgct acggtatccc
     1261 gtaacgcctg gcgtcgccac ccatgtcacc gactcccgcc gcagaattga aaaaatactg
     1321 aatggtgaag ataagcgact gttggtcatt attggcccct gctcgatcca cgatctcacc
     1381 gctgcaatgg agtacgccac ccgtctgcag tcgctgcgca accagtacca gtcacggctg
     1441 gaaatcgtaa tgcgcaccta ttttgaaaaa ccacgaactg ttgtcggctg gaaagggcta
     1501 atctccgatc cggatttaaa cggcagctat cgggtaaatc acggtctgga gctggcgcgc
     1561 aaattacttt tacaggtaaa tgagctgggt gtccctaccg cgactgagtt tctcgatatg
     1621 gtgaccggtc agtttattgc tgatttaatc agttggggcg cgattggcgc tcgtactacc
     1681 gaaagtcaga tccaccgcga aatggcttca gcactctcct gtccggtagg ttttaaaaat
     1741 ggtaccgatg gcaacacgcg gattgccgtg gatgctatcc gcgcagcccg cgccagccat
     1801 atgttcctct cgccagacaa aaatggtcag atgaccatct atcagaccag cggcaacccg
     1861 tatggccaca ttattatgcg tggcggcaaa aaaccgaatt atcatgccga tgatatcgcc
     1921 gcagcctgcg atacgctgca cgagtttgat ttacctgaac atctggtggt ggatttcagc
     1981 cacggtaact gccagaagca gcaccgtcgc cagttagaag tttgtgagga tatttgtcag
     2041 caaatccgca atggctctac ggcgattgct ggaattatgg cggaaagttt cctgcgcgaa
     2101 ggaacgcaaa aaatcgtcgg cggtcagccg ctcacttacg gtcaatccat taccgacccg
     2161 tgtctgggct gggaagatac cgaacgcctg gtcgaaaaac tcgcctctgc ggtagatacc
     2221 cgcttctgaa tgcgtgccca ttcctgatgg gatgggcatt tctgcgcaac ttgttgtctt
     2281 ctcaacacat tactgcttgc tctggcctgc cataatattg ataataagaa tcattgttat
     2341 atcaattatt attaattttt atgcgttata cggatagcag aaaactcacg cctgaaacgg
     2401 atgccaatca caagaccgct tccccgcagc ccatccggcg aattaccagc cagacactgt
     2461 taggtccgga tggcaaactg attatcgatc atgacgggca agaatatctg ctccgtaaaa
     2521 ctcaggctgg caagctgctg ttaactaagt agcctttaac tcgagcagct gacttccagc
     2581 cgtttacccc agtcaggtgg acgactgaca tagtcatcat ctctgtcgct gaaaggattt
     2641 cgcaacgcct catgcaggcg gtgcaattcc atcatatcac ccttttctgc cgcttcaatc
     2701 gcccgttgcg ccaaccagtt gcgcaatacc agagctgggt taacgctttg tatcagttgc
     2761 tgacgctcac tatcgctaac ctcgtcttgt tgcaaacgcc cccgataacg ggcaaaccag
     2821 tcatcaaatg ccgcacgatc aataaactca tcacgtagcg gtgacgccgc gctgtgctgc
     2881 tcggtcagac tcagcatgcg gaatgtgcgg gtataatcgc tgcgctctcg cgccatcaga
     2941 ctgaataatt cattcagtag cgcgttatcc tctttttgct ccgtcatgaa gcccagtttc
     3001 tgccgcatcc gttgtccata atgcgtcaac aaaacctgct gatagctgtc cagggcctca
     3061 ttcagggcat ctacggcaac aaatggtgac aatgtctgcg ccagacgctg taaattccac
     3121 aacgcgacag caggttgatt atcaaagcta taacgccctt gatgatccga gtgattacaa
     3181 ataaaaccgg gttcgtaatc atcaagaaaa ccaaacggcc cgtaatcaag cgtcagtccc
     3241 agcagcgaca tgttgtcggt attcatcacc ccatgtgcaa agccgaccgt ctgccattgg
     3301 gcaattaacg acgcggtacg tgcgacaaca tcgctaaacc agagacggta tttgtcctca
     3361 tcatcttcaa gatgtgacca gtaatgacga atagcaaagt cagctaactg acgaaccttt
     3421 tccgactcgc ggcggtagta aaaatgttcg aaatgaccaa agcgcagatg acttggtgcc
     3481 acacgcatca gcatcgcgcc tggctccgcc gtttcccgat acactggcga atcgctggtg
     3541 acgatactta acgcgcgggt cgtcggaatg cccagataat gcatcgcctc actggcgaga
     3601 ctttctcgta tcgtcgaacg taaaaccgcc cgtccatcac ccattcgcga ataaggcgtc
     3661 aggccagcac ctttcagatg ccagtccatt gtagtgccat cagcaagcag ttgttcgccg
     3721 agtaaaatgc cgcgcccatc acccagttgg cccgcccaga cgccgaactg atgaccactg
     3781 taaacctgcg ccagtggtga cataccaggc agtagggctt cgccgcccca gacacctgcg
     3841 ccatttttaa acagcgacga tggaatactc agcgtgttag ccagttcgat attatgccaa
     3901 atcagccggg cattatttaa aggcgtaggg gaaagtgctg tataggtttc tggcaattca
     3961 tcgcgccagc gggtaacaaa agacagggtc atagatcctc ctgtttgata gtgtagacgg
     4021 ttactctcgt cttaaacacc agcaaacaga agggttatcg ctgaaccaac gtcgttatct
     4081 gatttatggg aacggcaggc cacaggcatc cctggagagc atggaaatcg aagggggtaa
     4141 tctgtgccaa tatttctgcc gtatcaatgc ctcccgcaat gatgcaattg caacaagggg
     4201 aaatttgcgc ctggatcgca cgaataaacg gttcgaagga gcggtgcgtg atttgttgct
     4261 gaataaaact tttatccagc atgacgcggg tgaaaagacc atcaaaaacc gctttcattg
     4321 tgctattgcc tgctcccaaa ttacctaaca caaggggata cacttgcgat aaggataaaa
     4381 gaccgcgatt atcttttcct tcattgagat gcgggtaatt ctcattaatc aacagttcaa
     4441 taaacggata ttttaatagt tcgcctgcgt aattatccct ctctaataat aatgtagcca
     4501 cttgtggcgt taaattaagc caggcaaaga gtttatgttg aataaaaaaa tgttggcatg
     4561 attttagtaa ttccaactgc tctgaaaata attgccaatg ttgctcctcg gtaagttgcg
     4621 ctataacccg actcgtcggg atacgcaccg tgccatcttc actggagaaa tgggtaatta
     4681 actcaacgcc gacaaggact tgctggttat ctcttatcgg caggaaatag caatcagaat
     4741 gataaagatt ctccaaaaaa atcttcatgg taaccgtccc tctcgaagga tgtttttcag
     4801 tatccggcta aaagggatga ggccataaga cataacaatt acagaaggag taactttcat
     4861 ttgttccatg ttaaccactt tttcaggggt ctttttttag attatcctga ttataaacga
     4921 ataatccatt tacggaattt ttgtctgcaa aatactactg tattcaggag taaatggacg
     4981 gcgatgtatc atcgccgtcc atttttcgac atactagatt cgtctcgcct gccagaaatt
     5041 tttctgccaa tagacattat caagtgagga acgcatcact cccttgctgg tagaggcgtg
     5101 gataaattgg ttgttggtat cgtaaatacc tacatgcaaa ccattttgtc cggaccccgt
     5161 tttgaaaaag accaggtcac ctggcagcaa cttgtcttta tcaatttgcg tgccaataga
     5221 ggcttgttgt ttggtttcac ggggcagctg caaatcgaaa cgatcgcgca tcgtcacaac
     5281 cacaaatccc gaacagtcca caccgcgccg cgtcatgcca ccataacgat acggcgtgcc
     5341 atgccagctt tgtagctggt cgttcaaacc ggcaataacg gtaatcgaat cagaaagtct
     5401 ggcatttggc ggcggtgctt tatggtggct acacccggcc agaaacagtg ctgtgatcaa
     5461 aataaggcag aaacgcattc cgtacggttc ctctgttttt tattcttgca ttaatttagc
     5521 gtcgtaatta cccgattttc aagatactaa tgaaatcaga tggtcaaaat cagcattctg
     5581 tgaccttcga tatccagacg gcgaaaattc atcccatagg cctgcgccag atttggcggc
     5641 gtgagcacct cttctctgcg tccactggcc agcatttttc caccttttag caaccacgcc
     5701 cgatgcgcat gacgcaacgt gtggttgaga tcgtgactgc tcatcacaat cgccagtcct
     5761 tgctgacaca gcgcgctcag aattttgtct aacgcacttt gttgcgcaac atcaagactg
     5821 ttcatcggct catcaagaag cagcaattgg cctgcgggat tggcttgtgg tgtgatttgc
     5881 aacaccaccg cagcaagacc tacgcgttgc cattcaccgc cggaaagttg attggtgcta
     5941 cgaccgagtt tgtcatcaag agccagcgcc cctgcgacat cattcagtag ttcggtacgc
     6001 gttttatcgt gctgatgcag tgtcaggtag tgccagaccg gcattgcaaa cggcggcgtc
     6061 tgctgttgtg aaagataggc gcgatgcagc gcgagttttg ttgcggacca tgcttccagg
     6121 ggttgccccg cgaactgaat gcttccctta ccgctggtca ttccggccat tcgtgcaagc
     6181 aaggtactct tacccgcgcc attcggcccc accaggtgca ggatctcccc agcccgaacc
     6241 tcgccagaaa gtggccctag gcgggtagat tccgcaacat cctgtaactg catcacaata
     6301 gacattattt tgccaacgcc agtttaatgc tttccatcac aatggggcct tccggcgtca
     6361 tatccgggga aaaacgctgg atgaccaatc cgtccctgcc aactaaaaat ttctcaaaat
     6421 tccataaaat atcatcaggg tacagcggtg cacgaccttt gctgaccata cgggcataga
     6481 atccgctctc ttccggcgcg actgcggtcg gcgctgcggc aatcaatttt tgatacagcg
     6541 gatggcgtcc ttcgccgtta acttcaatct tactgaacat cgggaacgtc accccccatg
     6601 tggtggtaca gtaagtttta atctcttcat cgctgcccgg ttcttgttcc agaaactggt
     6661 tgcacgggaa tcccagcacc acaaaacctc gatcggccca ggctttctga atattctcca
     6721 actgctcata ttgcggcgtt aagccacact ttgaggcgac attgacaatc aacagcacat
     6781 taccggcgta cttctccagc gtggtcactt caccgtcgat atctttcact acggtcgtca
     6841 gaatggaatc ttgcatcgtt tctcctgggt gtggtcagta aaaatcttag cttttaatca
     6901 tagaccgtct ttttgcggct aacgtcttgc ttttaacaat aaccagataa acaccggcgc
     6961 acccaacgtt gcggtgacca cgccaataag cagctctgcg gcagctaatg ccaggcgcgc
     7021 tacaatatcg gccagcagca atgcgctcgc ccctgccagc gcgcagccgg gaagtaatac
     7081 gcgatgatcg gttaaaccac acaaccgaag aatatgtggg atcaccagac caataaagcc
     7141 gatagcaccc gccagcgcca cactgacgcc aaccatccag ccggtcgcgg ccaccagcac
     7201 attgcgccag aaccacaggg gtaaacccag ttgccgcgcc gagatctcgc caagtgctaa
     7261 catattcatc ggcctggact gacaacagat ccacaacaac acggggatca atgccagcat
     7321 cagccagctt tgccgccagt ctacgccgcc aaaaccgccc atcatccagt acatcagctg
     7381 acgtaaatca acagaggtgg aaaagtagat agcccacgtc attagtgcgc tacagataat
     7441 ccctaatgca acgccagcca gcaataaccg actggtcgaa agatgacgac gggcgaaacg
     7501 taagagtatt aaagtaatga taagcgcgcc agcaatcgca cacagcccta gcgcccagtt
     7561 ggggagttgc ccttgcccaa gcaataccgc ggcgataagc cccacgcctg cgccgttaga
     7621 gacgccaagt agtccaggtt ctgccagagg attttcaaac aacgcctgca ttacagcgcc
     7681 ggatatagcc agcgccgcac caaccaacaa tacagccagc gtacgtggca ggcgaatttg
     7741 ccagacgaac agttcgccac gaggagtaaa ccagtcacct agcgagatcc attgttcacc
     7801 ggcgcaaagg cttaaaagaa gcgccagcag catcaaaact gacaggcata ataaccagcg
     7861 aatattttgt cgctgttgtt ggcgggcaag tgtcagcatg gtatccgttc tgctgaagtg
     7921 tcatggcgtt gattttacgg tgactcttcg acagtgaaaa gaaaaaaggc cgcagagcgg
     7981 ccataaacac aagtaaaaat caacaagtta gataattatc aaagacttac agacacacaa
     8041 aaacacagcc aaccacaaca aataacaggg atgtggtcac tttgtggatc atatcgccgc
     8101 cataaattta cttaataaca gtaaccacag ccacaagcac ggccatcgca gacaggacaa
     8161 tacctgtgag taaccaagtc tgatttgcaa atgatttctg aagttcagta cgatgctcag
     8221 ccatctcaac tttcagagac tggcgtaaat cagccatctc agacttcagg gattggcgcg
     8281 attctgccat ctcaagcctc aacccttcgc gaatctccag tacatcagat ttggtagcaa
     8341 aggattcgct tcttgtggtg agagtaatca ggttgttttt gatttcagtc acatcacttt
     8401 ccaggcaact gacccgccgt tcaaggtcgt cattcatgcc atcacctcca tcattaccac
     8461 ttcctgttac ttggttgttg gatacattat aggcattctc tttctttttt ctgaaccgcg
     8521 aaatatgtgc aacattctct gccatcactt ctcatcaccc tgcttgccta cccagttcat
     8581 aaaggcccgg gccgagtaac gatgaacaaa cccacatctg ttacaggtaa gtcggaattc
     8641 atagttatgg atattttccc gatcatgtcc ctcatatccc ggatacccag taaatggacc
     8701 gatgtaatca agcataacaa aagtgcctaa atcacactcc tcagcagttt tcggctcagg
     8761 ccccacctca acaatattcg tcaaatacat gtacatatcc gtatcgccac acgacaaaca
     8821 tttttcattc gtagacgact cattgagaaa acgcgca
//
LOCUS       DAJVTY010000161         8750 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVTY010000161 DAJVTY010000000
VERSION     DAJVTY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.161"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="OQQ66_003435"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="OQQ66_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1906228.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="OQQ66_003436"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="OQQ66_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1906229.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="OQQ66_003437"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="OQQ66_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1906230.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEIGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="OQQ66_003438"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="OQQ66_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1906231.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMVGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="OQQ66_003439"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="OQQ66_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1906232.1"
                     /translation="MTAIKHPVLLWGLPIAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="OQQ66_003440"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="OQQ66_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1906233.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="OQQ66_003441"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="OQQ66_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1906234.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8351..8608)
                     /locus_tag="OQQ66_003442"
     CDS             complement(8351..8608)
                     /locus_tag="OQQ66_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906235.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
BASE COUNT         1905 a         2494 c         2431 g         1920 t
ORIGIN      
        1 cagcattacc gaaatcccca tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagatgc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataaccattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cgttcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct gtgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtt ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatgt gcgttttgat gcgcatcaac
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgctgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaaca ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga gcccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatttgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatagg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggacgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggcg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca ggggtcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca tggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgccgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggtgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccattgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctaagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggca ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tactgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcgctcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgccca caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccccataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcagccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcagttac gggacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcgatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aattaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccaa tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac
     8161 attcacggag ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag
     8221 acgccaacgt aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc
     8281 gatttttcta aaaagtgtgc gtttgctact acttaggtag gtgcagcttt cttaatcacc
     8341 ggcagccacg ttatacaggc cagttgatgg atcgattgtt atcaatgata tcttcatgag
     8401 tcggtgtctc acccagctta ccgaagctgg cataaagtga aggcagacgg gcccgtcctt
     8461 ctcccttttt cgccagaggg aaagcgcgaa gcatggtggc agcctcccag tcacaacaat
     8521 aggatggtgt gcacggctgc tgacgccatg attcagcgat agagccggaa aatacggggt
     8581 caaagccggt atcattaacc agagtcatcg tgacctgtac tgcggctgga tgatctcctg
     8641 ctactgcaac tgctaggcga ccgcggctcc cttcaggtag tctgttgtgg tcaactaaaa
     8701 ctggcctccg cgttagagtt tttccagtat cggttttctg attcgtttgg
//
LOCUS       DAJVTY010000162         8514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVTY010000162 DAJVTY010000000
VERSION     DAJVTY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.162"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1217)
                     /gene="rhsC"
                     /locus_tag="OQQ66_003443"
     CDS             complement(<1..1217)
                     /gene="rhsC"
                     /locus_tag="OQQ66_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCS1906236.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGTPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEAG"
     gene            1445..2053
                     /locus_tag="OQQ66_003444"
     CDS             1445..2053
                     /locus_tag="OQQ66_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1906237.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            2151..3542
                     /gene="selA"
                     /locus_tag="OQQ66_003445"
     CDS             2151..3542
                     /gene="selA"
                     /locus_tag="OQQ66_003445"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1906238.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVLNLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3539..5383
                     /gene="selB"
                     /locus_tag="OQQ66_003446"
     CDS             3539..5383
                     /gene="selB"
                     /locus_tag="OQQ66_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1906239.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            5573..6724
                     /gene="yiaY"
                     /locus_tag="OQQ66_003447"
     CDS             5573..6724
                     /gene="yiaY"
                     /locus_tag="OQQ66_003447"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1906240.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNSLKDACGFTNPIQATHEEIVAIYRAAM"
     gene            6889..8427
                     /gene="aldB"
                     /locus_tag="OQQ66_003448"
     CDS             6889..8427
                     /gene="aldB"
                     /locus_tag="OQQ66_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1906241.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKLARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1932 a         2214 c         2416 g         1952 t
ORIGIN      
        1 ccggcttcac cctgcgtgtg cagcacttca cggcggttca ggctgtcggt gatggtgatg
       61 cggtccttct catactgata cgtgtagctt aagcctgccg ggttaagctg ttctgtcacc
      121 cgcccgtcgc tgtcgtaacg gtaacggatt tccggccggc ccgtgtgacg gtgcgccacc
      181 atccggcccc ggtatttatc atcgtaagta aagctacgca cctgtgtatt gctgcggtca
      241 tacactgccg ccagttcgcc gcgcggcgtc cagccatagc gcaccagcgg cgcggcaggt
      301 aaattctccg ggtattccgg gtcgtgcgtc agccacacgg cagacagacg gatgccgttg
      361 tcccggccat attcggtgta acccggcagg gtatcaggaa aagcggacgg ttccgtcccg
      421 ccggaaatgg cctgctgccg ggcttcttct gcccgctgcg cctgcgtggt cagtaccagt
      481 tggaagtgac gcccggcacc atccgtcacg ccggtgattt cgccgctgaa ttcaccggcg
      541 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
      601 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      661 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      721 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      781 acgccgccgc gcaccagcca cagtgactcg ctgcggctgt aaccgtcctc accgggaaac
      841 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      901 agctgtaagc ggatatccgc aggcattttc cagccggggc cgaggctccc caccggcgcg
      961 ggcgtttttg tccggtaact gctgtaggtg cgggagagga tgaacggcag cgggccgggc
     1021 agggcgatgt cggtttcacc gggaaggacc tttgcaccga gcaggggatt gaccggatgg
     1081 ccggacgtca ctccgccggg gcacaccgaa caggccacgc cggtgggggt accaatgcgc
     1141 accccggctg aaccctgaac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgct
     1201 gccggttttc cgctcatttc tcatccttgt tttatcgggt tatttgtctg ctgacaggca
     1261 aaaccctaca gatttataag aaagcctgaa gtgagatatc catcagagtg aaataattag
     1321 gaaaatattt atatcaccat gataaatatg tgatttattt ggaagtaaca aaccatcaac
     1381 attttctgcc ctttttccca tcaccaggcc atactgatat ttttcctcgc acaaaggact
     1441 cggcatgaaa ctcgtcggta gctacaccag cccgtttgta cgcaaacttt ctattctgtt
     1501 gttagaaaag ggcataactt tcgaatttat taatgaactg ccctataacg cggacaacgg
     1561 cgtggcgcaa tttaacccgt taggaaaagt gccggtgctg gtgaccgaag agggcgaatg
     1621 ctggtttgat tcgccgatca tcgctgaata tattgagtta atgaatgtcg ctccggcgat
     1681 gttgccgcgc gatccgctgg agtcgttgcg ggtgcgcaaa attgaggcgc tggcggatgg
     1741 cattatggat gccgggctgg tatcggtgcg tgaacaggcg cgtccggcgg cgcagcagtc
     1801 tgaagatgaa ttgttacgcc agcgggagaa aatcaaccgc agtctggatg tgctggaagg
     1861 atatctggtc gatggcacac tcaaaaccga tacggtcaat ctggcgacta tcgccattgc
     1921 ctgtgctgtc ggatatctca atttccgccg cgttgcgcca ggttggtgtg tcgaccgccc
     1981 acacttagtc aaactggtcg aaaacctgtt tagccgcgaa agttttgctc gcaccgaacc
     2041 gccaaaggct tgatgcgcga tatgtcctcc tgacccatct cacgttacaa tccgtggtta
     2101 tgttaaacgc ccttctccat gtgagagggc cttgattagc caggtttcct atgacaaccg
     2161 aaacgcgttc cctctatagt caacttccgg ctattgatcg cttattgcac gatagctcct
     2221 tcctttcttt gcgtgatact tatggtcaca cccgcgtggt ggaattgttg cgtcagatgc
     2281 tcgacgaagc gcgagaagtg attcgtgaca gccagacgct tcctgcgtgg tgtgaaaact
     2341 gggcgcaaga agtcgatgcc cggttgacga aagaggcgca gagcgcgctg cgtccggtgc
     2401 tcaacctgac gggaaccgtg ctgcatacca accttggacg agctttacag gcggaagccg
     2461 cggtggaagc cgttgcgcag gctatgcgtt cgccagtgac cctcgagtac gatctggacg
     2521 acgctggacg cggacatcgc gatcgggcgc tggcgcaact gctgtgccgt attacggggg
     2581 cggaagatgc ctgtatcgtc aataacaatg cggcggcggt gttattgatg ttggcggcca
     2641 ctgccagcgg gaaagaggtg gtagtatctc gcggcgaatt ggtggagatt ggcggcgcgt
     2701 ttcgtattcc cgatgttatg cgtcaggcag gctgcaccct acacgaagta gggaccacca
     2761 accgcacgca cgcgaatgat tatcgtcagg cggtgaatga aaataccgca ctgttgatga
     2821 aagtacatac cagtaactac agcattcagg ggttcaccaa agcgatagat gaagcggaac
     2881 tggtggcgct cggcaaagag ctggatgttc ccgtagtgac tgatttaggc agtggctcgc
     2941 tggtcgatct tagccagtac ggtttgccga aagagccaat gccgcaggag ttgattgcgg
     3001 cgggcgtcag tctggtgagc ttctccggcg acaagttgtt aggcgggccg caggcaggga
     3061 ttatcgttgg taaaaaagag atgatcgccc gcctgcaaag ccacccgctg aagcgtgcat
     3121 tacgcgcgga taaaatgacc ctcgcggcgc tggaagccac gttgcgtctt tatttacacc
     3181 ctgaagctct gagtgaaaaa ttaccgaccc tgcgcctgct tacccgcagc gcagaggtca
     3241 ttcaaatcca ggcacaacgt ttacaggctc cccttgccgc acattacggc gcggagtttg
     3301 cggtacaggt tatgccatgt ctttcgcaga ttggcagtgg ttcgctgccg gttgatcgcc
     3361 tgccgagcgc ggcattaacg tttacacccc atgatggacg cggtagccac cttgagtcat
     3421 tagccgcccg ctggcgtgaa ttgccagtgc cggtgattgg tcgtatttat gacggacgat
     3481 tgtggctgga tttacgctgc cttgaagatg agcaacggtt tttggagatg ttgttgaaat
     3541 gattattgcg actgccggac acgttgacca cggcaaaaca accttattgc aggcgattac
     3601 tggcgtaaat gctgaccgtc tgccggaaga aaaaaagcgc ggcatgacca tcgatctcgg
     3661 ctatgcctac tggccgcagc cggatggtcg cgtgcctagt tttatcgacg tacccggtca
     3721 tgaaaagttt ctttccaaca tgctggcggg cgttggtggt atcgatcacg cgctgttggt
     3781 ggtggcgtgc gatgacggcg tgatggcgca gacccgtgag catctggcga ttttgcagct
     3841 gaccggtaac ccgatgctga cagtggcgct gaccaaggcc gatcgcgtgg acgaagcgcg
     3901 tgttgatgag gttgaacgcc aggtaaagga ggtgctgcgg gaatacggtt ttgctgaggc
     3961 aaaactgttt atcaccgcag caaccgaagg tcggggaatt gatgccctgc gcgagcatct
     4021 gcttcagtta ccaggacgtg aacacgccag ccaacatagt ttccgcctcg cgatcgaccg
     4081 cgcatttacc gtaaaaggtg ccgggctggt cgtcaccggt acggcgttaa gcggggaagt
     4141 gaaggtaggc gattcactct ggctgactgg tgtaaataaa ccgatgcgtg tacgtgcgct
     4201 gcatgcacaa aaccagccaa cagaaaccgc ccatgccggg cagcgtatcg cgcttaacat
     4261 cgcgggtgat gcggaaaaag agcagattaa ccgtggcgac tggctgcttg ccgatgcgcc
     4321 gccagagccg ttcacacggg tgattgtcga gcttcaaacc catacaccgc tgacccagtg
     4381 gcagccgctg catattcacc acgccgccag ccacgtcacg ggacgcgttt cactgctgga
     4441 agataacctt gctgaactgg tcttcgacac cccgttatgg ctggcagata acgaccgcct
     4501 ggtattgcgc gatatctctg cccgcaacac gctggccgga gcgcgcgtcg tgatgcttaa
     4561 cccgccgcgt cgcggtaaac gtaaaccaga gtatctgcaa tggctggctt ccctcgccag
     4621 ggcacagaat gatagcgaag cgttagccgt tcacctgcaa cgcggcgcgg ttaatcttcc
     4681 tgatttcgcc tgggcgcgcc agctcaacgg cgaagggatg cgcgaactgc tgcaacagcc
     4741 tggctatatt caggctggtt atagcttgtt gaatgcgccg gttgccgctc gctggcaacg
     4801 gaaaattctc gacacattag cgacttatca tgagcaacat cgcgatgaac ctgggcctgg
     4861 gcgcgaacgt ctgcgacgta tggcgttgcc aatggaagat gaagcgctag tactgttgct
     4921 gattgaaaag atgcgcgaaa gcggcgacat cctcagtcat cacggttggc tgcatctgcc
     4981 ggatcacaaa gcgggcttca gcgaagagca gcaggccatc tggcaaaaag cagagccgct
     5041 gtttagtgac gagccgtggt gggtgcgtga tctggcaaaa gagacgggta ccgacgagca
     5101 ggcaatgcgc ttgacgctaa gccaggcggc gcagcaagga ataattaccg cgatcgtcaa
     5161 agatcgttat taccgtaacg atcggattgt cgaatttgcc aatatgatcc gcgatctcga
     5221 tcaggagtgt ggttcaacct gcgcggcgga tttccgcgat cgcttaggcg taggccgaaa
     5281 gctggcaatt cagattctgg aatattttga ccgcattggc tttacgcgtc gtcgtggaaa
     5341 tgatcattta ttgcgcgacg cattattatt tccggaaaaa taaggaaatg attaattaat
     5401 ttttaaaata aattaataca aatttcttat gattttaaaa aaagcacatt gtttaatgaa
     5461 tacaatgtgc tttttattag attaattttg ataattaata ataatgatca tgttttcgaa
     5521 tgaaaatcct cagtaagctt cccacccttt tttacacttt caggagtgtg ttatggcatc
     5581 ttcaactttc tttattcctt ctgtgaatgt catcggcgct gattcattga ctgatgcaat
     5641 gaatatgatg gcagattatg gatttacccg taccttaatt gtcactgaca gtatgttaac
     5701 gaaattaggt atggcgggcg atgtgcaaaa agcactggaa gaacgcaata tttttagcgt
     5761 tatttatgat ggcacccaac ctaaccccac cacggaaaac gtcgccgcag gtttgaaatt
     5821 acttaaagag aataattgcg atagcgtgat ctccttaggc ggtggttctc cacacgactg
     5881 cgcaaaaggt attgcgctgg tggcagccaa tggcggcgat attcgcgatt acgaaggcgt
     5941 tgaccgctct gcaaaaccgc agctgccgat gatcgccatc aacaccactg cgggtacggc
     6001 ctctgaaatg acccgtttct gcatcatcac tgacgaagcg cgtcatatca aaatggcgat
     6061 tgttgataaa catgtcactc cgctgctttc tgtcaatgac tcctctctga tgattggtat
     6121 gccgaagtca ctgaccgccg caacgggtat ggatgcctta acgcacgcta tcgaagcata
     6181 tgtttctatt gccgccacgc cgatcactga cgcttgtgca ctgaaagccg tgaccatgat
     6241 tgccgaaaac ctgccgttag ccgttgaaga tggcagtaat gcgaaagcgc gtgaagcaat
     6301 ggcttatgcc cagttccttg ccggtatggc gttcaataat gcttctctgg gttatgttca
     6361 tgcgatggcg taccagctgg gcggtttcta caacctgcca cacggtgtat gtaacgccgt
     6421 tctgctgccc catgttcagg tattcaacag caaagtcgcc gctgcacgtc tgcgtgactg
     6481 tgccgctgca atgggcgtga acgtgacagg taaaaacgac gcggaaggtg ctgaagcctg
     6541 cattaacgcc atccgtgaac tggcgaagaa agtggatatc ccggcaggcc tacgcgacct
     6601 gaacgtgaaa gaagaagatt tcgcggttct ggcgactaat tccctgaaag atgcctgtgg
     6661 ttttactaac ccgatccagg caactcacga agaaattgtg gcgatttatc gcgcagcgat
     6721 gtaatcatca tttccacaac ggctggcaaa ttgtcagccg ctttttcaac tatctctgta
     6781 acccttgccc gtaaattcgt gatagccgtc gtaaagctgt taccgactgg cgaagatttc
     6841 gccagtcacg tctacccttg ttatacctca caccgcaagg agacgatcat gaccaataat
     6901 cccccttcag cacagattaa gcccggcgag tatggtttcc ccctcaagtt aaaagcccgc
     6961 tatgacaact ttattggcgg cgaatgggta gcccctgccg atggtgagta ttaccagaac
     7021 ctgacgccgg tgactgggca gctgctgtgc gaagtggcgt cttcgggcaa acgagacatc
     7081 gatctggcgc tagatgctgc gcacaaagtg aaagataaat gggcgcacac ctcggtgcag
     7141 gatcgcgcgg cgattctgtt taagattgcc gatcgaatgg aacaaaacct cgagctgtta
     7201 gcgacagctg aaacctggga taacggcaaa cccattcgcg aaaccagtgc tgccgacgtg
     7261 ccgctggcga ttgatcattt tcgctatttc gcctcgtgta ttcgggcgca ggaaggtggg
     7321 atcagtgaag ttgatagcga aaccgtggcc tatcatttcc atgaaccgtt aggcgtggtg
     7381 gggcagatta tcccgtggaa cttcccgctg ctgatggcga gctggaaaat ggctcccgcg
     7441 ctggcggcgg gcaactgtgt ggtgctgaaa ctcgcacgtc ttaccccgct ttctgtactg
     7501 ctgctaatgg aaattgtcgg tgatttactg ccgccgggcg tggtgaacgt ggtcaatggc
     7561 gcaggtgggg aaattggcga atatctggcg acctcgaaac gcatcgccaa agtggcgttt
     7621 accggctcaa cggaagtggg ccaacaaatt atgcaatacg caacgcaaaa cattattccg
     7681 gtgacgctgg agttgggcgg taagtcgcca aatatcttct ttgctgatgt gatggatgaa
     7741 gaagatgcct ttttcgataa agcgctggaa ggctttgcac tgtttgcctt taaccagggc
     7801 gaagtttgca cctgtccgag tcgtgcttta gtgcaggaat ctatctacga acgctttatg
     7861 gaacgcgcca tccgccgtgt cgaaagcatt cgtagcggta acccgctcga cagcgtgacg
     7921 caaatgggcg cgcaggtttc tcacgggcaa ctggaaacca tcctcaacta cattgatatc
     7981 ggtaaaaaag agggcgctga cgtgctcaca ggcgggcggc gcaagctgct ggaaggtgaa
     8041 ctgaaagacg gctactacct cgaaccgacg attctgtttg gtcagaacaa tatgcgggtg
     8101 ttccaggagg agatttttgg cccggtgctg gcggtgacca ccttcaaaac gatggaagaa
     8161 gcgctggagc tggcgaacga tacgcaatat ggcctgggcg cgggcgtctg gagccgcaac
     8221 ggtaatctgg cctataagat ggggcgcggc atacaggctg ggcgcgtgtg gaccaactgt
     8281 tatcacgctt acccggcaca tgcggcgttt ggtggctaca aacaatcagg tatcggtcgc
     8341 gaaacccaca agatgatgct ggagcattac cagcaaacca agtgcctgct ggtgagctac
     8401 tcggataaac cgttggggct gttctgatat aagaagctgg tcgcattggg tattcattgc
     8461 ctgatgtgac gcttacgcgt cttatcatgc ctacgggaac ctgaccgtag gccg
//
LOCUS       DAJVTY010000163         8490 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVTY010000163 DAJVTY010000000
VERSION     DAJVTY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.163"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..184
                     /locus_tag="OQQ66_003449"
     CDS             <1..184
                     /locus_tag="OQQ66_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1906242.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            403..645
                     /locus_tag="OQQ66_003450"
     CDS             403..645
                     /locus_tag="OQQ66_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906243.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(975..1904)
                     /gene="fdhE"
                     /locus_tag="OQQ66_003451"
     CDS             complement(975..1904)
                     /gene="fdhE"
                     /locus_tag="OQQ66_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1906244.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1901..2536)
                     /gene="fdoI"
                     /locus_tag="OQQ66_003452"
     CDS             complement(1901..2536)
                     /gene="fdoI"
                     /locus_tag="OQQ66_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1906245.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2533..3435)
                     /gene="fdoH"
                     /locus_tag="OQQ66_003453"
     CDS             complement(2533..3435)
                     /gene="fdoH"
                     /locus_tag="OQQ66_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1906246.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3448..6498)
                     /gene="fdnG"
                     /locus_tag="OQQ66_003454"
     CDS             complement(3448..6498)
                     /gene="fdnG"
                     /locus_tag="OQQ66_003454"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5911..5913),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1906247.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6692..7525
                     /gene="fdhD"
                     /locus_tag="OQQ66_003455"
     CDS             6692..7525
                     /gene="fdhD"
                     /locus_tag="OQQ66_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1906248.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7679..>8490
                     /locus_tag="OQQ66_003456"
     CDS             7679..>8490
                     /locus_tag="OQQ66_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS1906249.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQA"
BASE COUNT         2013 a         2152 c         2250 g         2075 t
ORIGIN      
        1 agaggcaatt aaacggctga acaagcttcg gggctggcgt aaggtttcac gatctgcaat
       61 tttacgcgaa gcggttgagc aatatctgga gcggcagcaa tttccggtgc gtaaagcaaa
      121 gggaggcagg caaaaggggg aggtagttgg tgttgatgat cagtgtaagg agcataagga
      181 atgaaaaaaa tttttcattc ttgaatataa aaaaacagat gcccttattc tggtattaat
      241 acaaggctgt tttacttgaa cttataataa ctgcaactgt tacatcgtat ctggaaaacg
      301 cctcgcaaaa ataaaaaatg atgcgtaaat gagctttctt taaagattcg gcaagggtac
      361 ttttctctcc atagattcaa aaatgatatt ggcgagatat ttatgaactc tctggcaggt
      421 atagatatgg gcagaatttt actcgattta tcgaatgagg tgattaagca acttgatgat
      481 ctcgaggtgc agcgtaatct tcctcgtgcg gacctattaa gggaagcggt agatcaatac
      541 ctgataaatc aatcgcaaac agcaagaacc agtgttcctg gcatctggca agggtgtgag
      601 gaagatggtg tcgaatatca gcgtaagctg cgcgaggaat ggtaatggga aaaaatgatg
      661 tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg atgcggcgtg aacgccttat
      721 ccggcctaca aagtcatgca aattcaatat attgaagttt attggtaggc ctgataagcg
      781 cagcgcatca ggcaatcata cgcttgtcat cagtgacata cccataacct tatcgatgtg
      841 ttggagggaa gaacgaggca aacagacaat tgcggcatga tcttgaatgg gtaacgcgga
      901 atacgagaga tggtgtcgtc acaccctatg agctgtaatc cgggcgacca gcgccacccg
      961 gaaaacgtca gagattactc cccttcaccc ggaaacagga acgggttgat ggaactgcgg
     1021 gcatagcctt cttgctccat tcgcgcgtcc agtaccagag aagcgaggtc atctgccacg
     1081 gcttcaactt tcggttcttt ttcctgataa agaatcttca ggtaagtgcc gcagtcatcg
     1141 cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg accagtaatg cagtttgccg
     1201 ctctgttcac agttgctgca ttttacgcgc actacgtgcc attcggtttc acacaggttg
     1261 cagtgcaggt aacgcagacc ctgagtggtg ccaatttgca ccatgctgga caccggcata
     1321 gaaccacaaa ccgggcaata ttgacgttgc tcgccgtatt cagcgcgggc tttgccgggg
     1381 atcagattgg ccatctgcgc ccagtagagc gacagtgcag cccagataaa cggcgcttta
     1441 tcgctgctga cggatgagaa atcagaggca aacagtgcgc tggccatatc ttccagctcc
     1501 tgagtcgatg ccttctccag attctcaatc actgccagcg ccgggccgct catttcaggt
     1561 ttcagctcag caatcagcgc catcagcagc ttttgccagt gcttatcacg cggcagaacg
     1621 tgaatatcca gcgggggctt gccctgtgcg ctggcttctt taatgcgcgc ggtcagatcc
     1681 atctccagcg gatggtcgta cagcaccact tcctgggcgt gggcgataag cgcagcaaag
     1741 cgcaggtaat catccagcgg attattttct gccagctcgc gcagacgctc ggcgcggcgg
     1801 ttgtataaat tcttgagccg agggaacaat aacggcggaa tcatatccgc cgtacgtttc
     1861 tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac tcattcagct ttcttttccg
     1921 ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt cttcgcccag gcgctggtaa
     1981 cccatccttc caccatggcg gtaatcgtgc ctttcaccca aagggcggcg tagatatgca
     2041 ccatgataac cacaattaac gccactgcgg caaatgaatg cagcattaac gcgaatcgga
     2101 tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca gataatcacg ccgctcacca
     2161 gcagcagaac caggaaaata atcgccgccc agaaaacgca tttctgaccg aagttataac
     2221 gcccggtgtc acctacttcc tcgttgacga cgatcttacg aatattcttc gcccaaaaga
     2281 tatcatcccg attgattagg ttgtgatgcc agtaacggaa aaacatgatg atgaacgagg
     2341 caaacataac cacgccgaca aacgggtgca gaattcgcgc cagctgcggt gtgcccatga
     2401 tttgcatcaa ccagttgaag gacgggaaca aaaagcccag cccgctcacc gccgctagga
     2461 tgaagcagaa ggcggtgatc cagtggttga tacgttccgg cgcggtgtag cgcacgatgg
     2521 tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg tgcagattat tctcttcctc
     2581 atccgcacgg ttcggaccga cacccacgta gtggaagata ctggctgcga aggtagccgc
     2641 aaagccaaca gctgcgagcg gtttccagat gcctttccag aatttcacgg tttcgctgat
     2701 ttccgggttc tccggcaagc catgatacag atttggcttg tcagcatggt gcagcacgta
     2761 cataacgtgt gtaccaccga cgcctgccgg atcgtacagg cccgcattgt cgtaaccgcg
     2821 ggttttcagc tccgccacgc gctcgctcgc cagcgttttc atcgactctt tcgtaccgaa
     2881 gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt tcttgcccaa ccaccacgcg
     2941 gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc gggttgaggc gcggaatgtc
     3001 gaacggacag cccgcaatgc aataaccgca gccaatgcac tgctcggact ggaagtcgac
     3061 gataccgttg gcatactgaa taattgcacc ttccgccggg cacgctttca gacagcctgg
     3121 atcggaacag tgcatacagc cgtctttgcg gatcagccat tccagtttgt cgttctgctc
     3181 cacttccgag aagcgcatca ccgtccacga tttggcactt aaatcattgg ggttgtcgta
     3241 caccccaatg ttattgccga cggtatcgcg aatgtcgttc cactctgaac acgccacctg
     3301 acaggcttta cagccgatac aggtggtgac gtcgatgagt ttcgccactt cttcctggaa
     3361 gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg gaacgacgaa tgatatcttg
     3421 agattgataa gccatatgtc gtctccgtta caccttttcc acattcacaa ggaaggactt
     3481 aaactccggc gtctgcgtgt tcgcatcacc aacgaatggc gttaaagtat tggcaataaa
     3541 gcctttttta gctacgcctt catagcccca gtgaatcgga ataccgatgg tatcgatatc
     3601 tttgccgttc gctttcagcg tgcgaatacg tttggtcacc accgctttgg ctttgatata
     3661 gccacggttg gaggagactt tcacggtatc gccctgggca atgccaagtt tattcgccag
     3721 cgactccccg atttccacaa actgctctgg ctgcaaaatc gcgttcaaca gcgcgtgttt
     3781 ggtccagtag tggaagtgct cggtcagacg ataggtggtt ccgacatacg ggaacttatc
     3841 ggctttaccc aatgcttcgg cgtcgtcttt aaagatacgt gcagccgggt tcgagataac
     3901 gtttggatgc agcgggttag ttcccagcgg cgtttcaaac ggctcgtagt gttccgggaa
     3961 cggaccttcc gccatcttat cgagggcaaa cagacgcccc atgccttcct gctgcatgat
     4021 aaacggcccg acgccgctac ccggaggcgc tgcgctgtag tccggaatat cccagccggt
     4081 ccacttagtg ccgtcccatt tcagcaactg acgcttcgga tcccacgggt taccctgcgg
     4141 atctgcggag gcgcggttat acagaatgcg gcggttaagc ggccatgccc atgcccagcc
     4201 cagcgtgtta ccgaggccag acggatcggc gttatcacga cgcgccatct ggttgccttc
     4261 cggcgtccag ctaccggcga aaatccagca gccacaggag gttgtaccgt catcgcgcaa
     4321 ttgggcgaac gagctaagtt gttggccttt cttgacgata accgcaccgg ttgccggatc
     4381 ggtaatatcg gccagcgcct taccgttgct ctccatcgct acttcttccg acttcggctc
     4441 atgcggaatg gcgtagttcc aggtcatgtt cagcacctga tccgggttcg cgccaccctg
     4501 ttcggcatac atcttgcgca agcgcaggaa gataccggag aggatctcac cgtcagtcag
     4561 cgcaatcccc ggggcgtccg cacctttcca gtgccactgc aaccagcgac ctgagttaac
     4621 gattgaaccg ttctcttctg cgaagcaggt cgatggcaga cggaacactt cggtctggat
     4681 cttcgacgag tcaacttcgt tcagctcacc gtggttctgc cagaagttag aggtttcagt
     4741 gttcagcggg tcgatggtga cgaggaactt cagtttcgac agacaaccga tcactttgtt
     4801 tttgttcggg aatgaggcaa cagggttaaa accctggcag atatagccat tgaccttgcc
     4861 ctctttcatc atctcgaagt attgcaggac gtcgtagcct ttatcccact tcggcaacca
     4921 gtcaaagccc cagctatttt ccgccgtcgc tttatcacca aagaaggcct tcatcataga
     4981 gacgaagaat ttcgggtagt tgccccagta gtttacctgg ccttccagca gcggttttgg
     5041 cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc tcgcttggca gcgtcatgta
     5101 acctggcagg ctctgcgaca gcagccccag gtccgtcagc ccctgaatat tggagtgacc
     5161 gcgcagggcg ttaacgccgc cgcctgccat ccccatattg ccgagcagca gctggatcat
     5221 cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc gtccagccga gggcatacag
     5281 gaacgaggca gttttatcgt gagcactggt ttctgcgatg tattcgcaga ctttcaggaa
     5341 cgcgtctttt ggcgtaccac agatgttttc aaccacatct ggcgtgtagc gggaaacgtg
     5401 ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc gtggtatcgc gtttggcgaa
     5461 gccgttttcg tccagttcgt aagtccagga ggatttatcg tacttgcgtt tttccgcgtc
     5521 atagccggtg aaaaggccat cttcaaagcc gtaatcctca cgcacgatca ggctggcgtt
     5581 ggtataggct tcggtgtatt cgcggttgaa tttttcattg ttcagcaggt acagcaatac
     5641 gcctgacagg aaagcaatgt cagtaccgga acgaataggg gcatagtagt tagctaccgc
     5701 cgccgtacgc gtaaagcgag gatcgatcac aatcagcttc gcgccgttgt gaattttggc
     5761 ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg gcgttaccgc ccatcaccac
     5821 gacgaggttg gcgttcttga tgtcgaccca gtggttggtc atcgcaccgc gaccaaatgt
     5881 tggagcaaga cttgctaccg ttggtccgtg tcagacacgc gcctggttgt cgaccgcgag
     5941 catacccagc gcgcgggaga atttttgcgt taaatagccg gtttcgttac tcgacgcgga
     6001 agcacacagc atcccggtgg agagccagcg gttaacagtc acgccttcgg cgttttgcgc
     6061 aatgtagtta gcatcgcggt cttctttcat cagtttggcg atgcgatcaa acgcctcttc
     6121 ccaactgatt tgctgccatt tatcagaacc tggcgcacgg tattccggaa acttcagacg
     6181 gctttcggag tggatgaaat ccaccaggcc agcgcctttc ggacaaagtg caccgcggtt
     6241 gaccgggtga tccggatcgc cttcgatatg gaagatagat gctttggcgt tttttgctcc
     6301 gtcaccgagg ctgtacatca acagcccaca gcctacggaa caataggtgc aggtattacg
     6361 ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc gcgagtgcta cgctgggtgc
     6421 aaaacctagt gccgctgccg tggtgcctgc cataccgcca gcgcagatct taaagaactg
     6481 ccttctgctg acctgcatgg attgctcctt gtttcgacat tgtcacgtcc catttacatt
     6541 cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt gcggaagggg ccgcaaaggt
     6601 ccagaattgg ctcgatttcc ctccatccag gaagggtttg taacagaata ccataatgtt
     6661 ggtgtgtgtg ttcttatctg gttaagagaa agtgaaaaaa acacagcgaa aagaaatcga
     6721 aaatgtgaca aatatcacag gtgttcgtca aattgagtta tggcggcgtg atgatttaca
     6781 acacccacgg ttagatgagg tcgcggaaga agttcccgtt gcgctggtct acaacggcat
     6841 ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag tactttgcgc tcggtttttc
     6901 gctttccgag gggattatcg aaagtccgcg cgatatcttc ggcatggatg tcgttccttc
     6961 ctgtaatggt cttgaagtac aaattgagct ttccagccgc cgctttatgg ggttgaagga
     7021 gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta tgcggcgtgg agcaacttaa
     7081 tgacatcgga aaaccggtgc agccgctacc gttcacccag acgtttgatc tcaacaaact
     7141 ggatgatgca ttacgtcatc tcaacgattt tcagccagtg gggcaactga ctggttgtac
     7201 tcacgccgct gcctggatgt tgccatctgg cgaactggtc ggcgggcatg aagacgtggg
     7261 gcgccatgtg gcgctggaca aactgttagg ccgccggtca caagaagggg aaagctggca
     7321 gcaaggtgcg gtactggttt ccagtcgtgc cagttatgaa atggtgcaaa agtcggcgat
     7381 gtgcggcgta gagattttgt ttgcggtgtc tgccgcgacc acgcttgctg tagaagtggc
     7441 tgagcgctgt aatctgacac tggtaggttt ttgtaaaccg ggtcgggcaa cggtttatac
     7501 ccatccgcag cgtttgagca attaattaaa ataagtcctg cgatatatat taaattgcag
     7561 gacttattca tttcgtgaat tttattattt ttatttataa ataaaacgcg tgtagtggcg
     7621 ttttcttttt tcatttatta tgttcactct cttatctata tatttataag gcaattaaat
     7681 gaaaaggaat ttattatctt ccgctattat agtcgccatc atggccctcg gtctgacggg
     7741 ttgtgatgat aaaaaagccg aaacagaaac gcccccgcct gccaatagtc aacctgccgc
     7801 accagctcct gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc
     7861 ggtggtcgat gaacaagcgg ttttcgacga aaaaatggat gtctatatca agtgctataa
     7921 caagttacag atccctgtac agcgcagtct ggcgcgttat gttgactggc tgaaagattt
     7981 taaacagggg cctaccggtg aagagcgtac tgtttatggc atctacggca ttagtgaatc
     8041 caacctcgct gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca
     8101 accaattgat ggcgtggcgg tgagttatat cgatgctgcc gtggcgttgg gtaataccat
     8161 taacgaaatg gataaatatt acacccagga aaattataaa gacgatgcgt ttgcgaaagg
     8221 gaaaacgctg caccagacat tcttaaaaaa tctggaagcc tttgaacctg tagcagaatc
     8281 ttatcatgcg gcgattcagg aaattaacga taagcgccag cttgcggaac tgaaaaatat
     8341 tgaagaaaga gaagggaaaa tattccacta ctattctctg gcagtcatga tttcagcgaa
     8401 acaaattaat aacctgatat cgcaagagaa gtttgatgtt gatgcggcaa tgaagaaagt
     8461 gtctgaactg gaaacgctgg tggcgcaggc
//
LOCUS       DAJVTY010000164         8312 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVTY010000164 DAJVTY010000000
VERSION     DAJVTY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.164"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(95..1765)
                     /gene="hscC"
                     /locus_tag="OQQ66_003457"
     CDS             complement(95..1765)
                     /gene="hscC"
                     /locus_tag="OQQ66_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS1906250.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSTQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="OQQ66_003458"
     CDS             complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="OQQ66_003458"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1906251.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="OQQ66_003459"
     CDS             complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="OQQ66_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1906252.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKVPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="OQQ66_003460"
     CDS             complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="OQQ66_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1906253.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="OQQ66_003461"
     CDS             complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="OQQ66_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1906254.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="OQQ66_003462"
     CDS             complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="OQQ66_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1906255.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6574..>8312
                     /locus_tag="OQQ66_003463"
     CDS             6574..>8312
                     /locus_tag="OQQ66_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS1906256.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLR"
BASE COUNT         2075 a         2053 c         2076 g         2108 t
ORIGIN      
        1 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
       61 gatgcagcgt ttacgccgca tccggcagcc gtgtttatgg gatctcaatg gctaaataat
      121 cgctcgcccg ccgccgaact tcatccacgc gggccatgtc gtttgactgt agcgccgcat
      181 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgct cgcgcccaca
      241 actcttccag tttggcttta aaggtgcgat tgatgagcat atcgcgcgga taaattttca
      301 atgcggataa ccgcgtccga ctctcttcaa tctgctgcgt gctcaatgtt accgggctgt
      361 ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact tcaagcaacc
      421 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
      481 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
      541 cgttaaccgt aattgaatcc tgctccgggt gcatggtcga gtaagtttct acacgcgaca
      601 ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg ccctggcggt
      661 taacttcaac gcccaacgag taagggcaaa tatcagtgag gattacctct tcaatatctt
      721 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcggat
      781 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
      841 tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgcgcat
      901 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
      961 ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcttc ctgatactgc cagcgaatgt
     1021 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctgccagtt
     1081 cactctcgtt aagcgtggtc ctggcaactt ccgcgcgttt taaaacctca tcgaccagca
     1141 tatgggtaaa atcttcgcca ccgaggaagc tatcgccagc ggaggcgtga atttcaatca
     1201 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaaaacca
     1261 gcgagcgggt attttgttgc gtatgcaggc catatgccat cgcagctgct gtgggttcat
     1321 taattaagcg taccgcattt aacccggcta actccgcagc taaacgggta tgcttgcgtt
     1381 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     1441 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     1501 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcgcgttta aataacgctg
     1561 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     1621 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     1681 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     1741 caatggtgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     1801 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     1861 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     1921 ggcattcggt ttattgtcag tcagataata ataatcaaca accgtcatac cctgggtata
     1981 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     2041 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     2101 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     2161 aatcgcgcgg aaacgctcgg tgtcttcaat gtggatttgc gctttatgag taacatccag
     2221 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     2281 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     2341 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     2401 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     2461 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     2521 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     2581 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     2641 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     2701 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     2761 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     2821 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     2881 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     2941 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     3001 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     3061 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     3121 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     3181 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     3241 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcacttt
     3301 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     3361 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     3421 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     3481 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     3541 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     3601 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     3661 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc
     3721 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     3781 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     3841 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     3901 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     3961 tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata
     4021 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     4081 cacagcaaaa ccataactaa aggaatcgag cggaatatgt taacgtaggc tttggcaaac
     4141 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     4201 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     4261 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     4321 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataatc gcgttaatca
     4381 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     4441 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     4501 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     4561 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     4621 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     4681 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     4741 taaaccacat gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga
     4801 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     4861 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     4921 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     4981 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     5041 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     5101 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     5161 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     5221 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     5281 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     5341 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     5401 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     5461 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     5521 acggcacgac cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg
     5581 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg
     5641 gaagtgacga ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt
     5701 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     5761 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     5821 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     5881 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     5941 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca
     6001 ccgtttttgg cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt
     6061 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     6121 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     6181 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     6241 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     6301 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     6361 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     6421 atacttaggg gcaaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     6481 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     6541 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     6601 aatgttcaat tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     6661 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc
     6721 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     6781 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     6841 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     6901 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgata gtgatcagat agtcatcagc
     6961 gttggggcat ccggggcaat tatgggaata gctggcgctg cgatagcaac acagcttgct
     7021 tcaggtgcgg gcacatacca taaaaaccag cggcgagtat tttctctgtt gggtatggtg
     7081 gcgctgacac tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc
     7141 ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga
     7201 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     7261 cttgcgcagc agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg
     7321 ttttatccac aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa
     7381 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     7441 ttgctcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg
     7501 tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc
     7561 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     7621 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact
     7681 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg
     7741 attaccaccg gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg
     7801 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg
     7861 gcggatatcc cgctggagaa atacgacggg gcggggacga gcggtaaacc aggcgcctgg
     7921 gttatggcac tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac
     7981 atcgtgcgga tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt
     8041 ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgcagatagt
     8101 caggggattt cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt
     8161 agcaggagcg tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg
     8221 tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc
     8281 gtttatcctg ccagctacgt taatcacctt cg
//
LOCUS       DAJVTY010000165         8312 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVTY010000165 DAJVTY010000000
VERSION     DAJVTY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.165"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..201
                     /gene="csgC"
                     /locus_tag="OQQ66_003464"
     CDS             <1..201
                     /gene="csgC"
                     /locus_tag="OQQ66_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1906257.1"
                     /translation="SLREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIV
                     VTVSDGQALHLSQQWPPSSEKS"
     gene            322..633
                     /locus_tag="OQQ66_003465"
     CDS             322..633
                     /locus_tag="OQQ66_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1906258.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            729..1262
                     /gene="ymdB"
                     /locus_tag="OQQ66_003466"
     CDS             729..1262
                     /gene="ymdB"
                     /locus_tag="OQQ66_003466"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1906259.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1204..2685
                     /gene="clsC"
                     /locus_tag="OQQ66_003467"
     CDS             1204..2685
                     /gene="clsC"
                     /locus_tag="OQQ66_003467"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1906260.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2693..3850)
                     /gene="mdoC"
                     /locus_tag="OQQ66_003468"
     CDS             complement(2693..3850)
                     /gene="mdoC"
                     /locus_tag="OQQ66_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1906261.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4244..5779
                     /gene="mdoG"
                     /locus_tag="OQQ66_003469"
     CDS             4244..5779
                     /gene="mdoG"
                     /locus_tag="OQQ66_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1906262.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5772..>8312
                     /gene="mdoH"
                     /locus_tag="OQQ66_003470"
     CDS             5772..>8312
                     /gene="mdoH"
                     /locus_tag="OQQ66_003470"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1906263.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2094 a         1973 c         2179 g         2066 t
ORIGIN      
        1 tccctgcgcg aaggcggttc agggcaaagt cagacgaagc aagaaaagac cctctcattg
       61 cctgctaatc aacctattgc tttgacgaag ttgagtttaa atatttcccc ggaagatcgg
      121 gtgaaaatag ttgttactgt ttctgatgga caggcacttc atttatcaca acaatggccg
      181 ccctcttcag aaaagtctta atttgttgaa atgtcgagca taagatgaat ctggagagaa
      241 tggtctgctg cgaatcagcc aacctgaaag tatggataac acaaccctca aggatgacta
      301 atcattgagg aaatagaata aatgttcaga ccttttttaa actctcttat gctcggcagt
      361 ttgttttttc cttttattgc catcgcagga agcaccgcgc aagggggcgt gatccatttt
      421 tatggccaaa ttgtggaacc ggcatgtgac gtcagcaccc agtcatcacc cgtagaaatg
      481 aactgcccac agaatggttc tgttccggga aaaacctatt ccagcaaagc gttaatgagc
      541 ggcaatgtca aaaacgcgca agtagcctca gtaaaagtgc agtatattga taaacagaaa
      601 aagctggctg taatgaacat cgaatataac taagtttctg aacaacgcac gttgctgagt
      661 agaaaaatgc gatttaacca aaaagcctgc tgtacactta agaaacaaga aggtgttaag
      721 gaggcgttat gaaaacgcgt attcatgttg tgcagggtga tattaccaaa ctggccgttg
      781 atgtgattgt gaatgcggct aatccgtcat taatgggagg cggcggcgtc gatggggcca
      841 ttcatcgcgc agcgggtccg gccctgctgg atgcctgttt aaaagtcagg caacagcagg
      901 gcgattgccc tacggggcat gccgttatta cgcttgcagg cgatcttccc gctaaagccg
      961 tagtacacac cgtcgggcca gtctggcgtg gtggtgaaca aaacgaagac cagcttttgc
     1021 aggatgccta tctcaatagt ctgcgactgg tggcggcaaa cagctatacg tcagtggcat
     1081 ttcctgcaat cagtactggg gtttatggtt accctcgtgc ggcagcggct gaaatcgcag
     1141 taaaaaccgt ttcagaattt attacccgtc acgctttacc cgaacaggta tactttgtct
     1201 gttatgatga agaaaacgcc cacctctacg aaagactcct tacccaacaa ggagatgaat
     1261 gatttgcccc ggctggcgag cgcggtgctg ccactgtgtt cgcaacatcc cggtcagtgt
     1321 ggtcttttcc ctctggagaa aagtctggat gcgtttgccg cccggtatcg tctggccgaa
     1381 atggcagagc atacgctgga tgttcagtat tacatctggc aggacgatat gtcgggtcgg
     1441 ttactgtttt ccgccctgtt agccgcagca aagcgtggcg ttcgcgtccg tttgttgctg
     1501 gacgacaaca atacgcccgg acttgacgat attttacgct tgcttgacag ccatccccgc
     1561 attgaagtcc ggctttttaa tcctttctcg tttcgcttgc tgcgtccgct tggttatatc
     1621 accgactttt cccgtctcaa tcgccgtatg cacaataaaa gtttcactgt cgatggcgtg
     1681 gttaccttgg tgggaggacg aaatattggt gatgcctatt ttggggcagg ggaagagcca
     1741 cttttttcgg atttagatgt tatggcaata ggacccgtgg tagaggacgt tgccgatgat
     1801 ttcgcccgct actggtattg caaatcggtt tcacccttac agcaggtgct ggatgtcccg
     1861 gaaggtgaaa tggcggatcg catcgagtta cccgcctcct ggcataacga tgccatgacg
     1921 catcgttact tacgccaaat ggaatccagt ccgtttataa atcatctggt tgatggaacg
     1981 ttgccgctta tctgggcgaa aacacgttta ttaagtgatg atccggcgaa aggggagggc
     2041 aaggcaaaac ggcattcact gttaccgcag cgcctgttcg atatcatggg ctcacccagt
     2101 gaacgcatcg atattatctc ttcctatttt gtaccgacac gcgcaggtgt ggcgcaactc
     2161 ttacggatgg tgagaaaagg ggtaaagatt gcgatcctaa ccaattctct tgccgctaac
     2221 gatgttgctg tcgtccatgc cggatacgcg cgctggcgca aaaaattgct ccgctatggc
     2281 gtggaattat atgaactcaa gccgacgcgt gaacaaagta gtacgttaca cgatcgcggc
     2341 ataaccggta attccggtgc cagcctgcat gctaaaacct ttagcatcga tggtaaaacg
     2401 gtgtttatcg gctctttcaa tttcgatcca cgttcgacat tgctcaatac tgaaatgggc
     2461 ttcgtgatag agagcgaaac gctggcacag ttaattgata aacgctttat acagagccag
     2521 tatgatgcgg cctggcagct ccgtctggac aggtggggac ggatcaactg ggttgatcgt
     2581 catgcaaaga aagagattat tctcaaaaaa gaacccgcca ccagtttctg gaagcgggtt
     2641 atggtcagac tggcgtcgat attgcccgtg gaatggttat tgtaaatgtg gcttaacggg
     2701 ctggtgcttt atcgttctca cgcttaacaa ccggtttacc agaaaacaaa aacttcagta
     2761 acgggatgcg taaatgaatt tcatacagaa ttatcgcaat ccctactacg aatatcaggc
     2821 cacagagaaa accaagccag ttggaggtga tgtgcggtgt aatgtatgcg ccgaaaaaca
     2881 gcgttaacgg gtggtgaacc agatagataa atagcgatgc gttaacaaag taagtcaccc
     2941 gcgctgactg gaagttaagc aaacggtggc caaaggagaa gaccacattc accatccaca
     3001 gaccgaggac catggtgatc accgactcgg tttcgtacat ccaggcatcg ccactgccat
     3061 agcgttggtt gagtaaataa gcgacaaatg ccaatgctgc tgcaagggta cagccacgag
     3121 acggcgtggt aaacaaggct ttaagatgag ggaaaatgaa agccagtgca ccgaggataa
     3181 agaacggcaa ataaaatagc gtttgcatga caataaaatt gaacatgcca ttactcagaa
     3241 tgggcggata cacaataaaa atcgttcttc ttattaccgc atagccgatg ccgaggcata
     3301 aaaaaatcac cgatagtttt accatcgaga attttttatt cgttttatca gaattttcta
     3361 aattatttct gatgcgttta aatatccata cgcacagcgt cgtcatgact actaacacca
     3421 gtaaaaacca caggtgtgat attaattccc aggccaacgt attatatttg tcatacaatg
     3481 acagcccagg ccaactttcc gctttccctt tgacgtattg cagcataata aattgcggca
     3541 atgtcagtaa ggggatggct gttaacatcg ggatacctac acgttcgaca cgtactttcc
     3601 accatttttt caagggatag cgtaaaaaaa gcatgtagga aaagtagccg gatataacga
     3661 aaaataccag catgcggaac gagtggatga agtcattaaa aagggtcagc cacaatgacg
     3721 gttcggcgct attcacatgc catgtatggc tcgaatagat taaagaaata tgaaaaggga
     3781 tccctaacaa catcagccag gcgcggatgg agtcgaggaa atattcacgt tgcgcgggta
     3841 ctggattcat atatggttaa ctaatctcgg atttttcgtc ttatccctgt cgggttatgc
     3901 ctttaggctt gttgccatag cgacaccgac ctgaccgcgc caggcgcagg cttcaaggtt
     3961 tttatgcata gcataatcgc taccactaac cagaatggaa gcgtctgtaa gacggttgat
     4021 aaataaaatt gctggcaaac cctacacgaa gtcgatgctt ctgtctttag gagaagcacg
     4081 gaaagtgaaa acggttgcaa tcaggtgctt aatccatgag ccagcgtgct gaacgatacc
     4141 gggattctgt tgtcggaatg gctggttatc cattaaaata gatcggatcg atataagcac
     4201 acaaaggggg aagtgcttac taattatgaa acataaacta caaatgatga aaatgcgttg
     4261 gttgagtgct gcagtaatgt taaccctgta tacatcttca agctgggctt tcagtattga
     4321 tgatgtcgca aagcaagctc aatccttagc cgggaaaggc tatgaggcgc ccaaaagcaa
     4381 cttgccctcc gttttccgcg atatgaaata cgcggactat cagcagatcc agtttaatca
     4441 tgacaaagcg tactggaaca atctgaagac cccattcaaa ctcgagttct accatcaggg
     4501 tatgtacttc gataccccgg tcaaaataaa tgaagtgact gccaccgcag tcaaacgaat
     4561 caaatacagc ccggattatt tcactttcgg cgatgttcag catgacaaag acacggtaaa
     4621 agaccttggt tttgccggtt ttaaagtgct ttacccgatc aacagcaaag ataaaaacga
     4681 tgaaatcgtc agcatgctcg gggccagcta tttccgcgtg attggtgcag gtcaggttta
     4741 tggcctttct gcccgcggcc tggcaattga taccgccttg ccatcgggtg aagaatttcc
     4801 acgcttcaaa gagttctgga tcgagcgtcc aaaaccgact gataaacgtt taaccattta
     4861 tgcattgctt gactcgccgc gcgcgacagg tgcttacaaa ttcgtggtta tgccagggcg
     4921 tgacacggtt gtggatgtgc agtcgaaaat ctatctgcgc gataaagtcg gcaaactggg
     4981 gggtgcaccg ttaaccagta tgttcctgtt tgggccgaac caaccgtcgc ctgcaaataa
     5041 ctatcgtccg gagttgcacg actctaacgg tctctctatc catgccggta atggcgaatg
     5101 gatctggcgt ccgttgaata acccgaaaca tttagcggtt agcagcttct cgatggagaa
     5161 cccgcaaggc ttcggtctgt tgcagcgcgg tcgtgatttc tcccgctttg aagatctcga
     5221 tgatcgttac gatcttcgtc caagcgcatg ggtgactccg aaaggggagt ggggtaaagg
     5281 cagcgttgag ctggtggaaa ttccaaccaa cgatgaaacc aacgataaca tcgtcgctta
     5341 ctggacgccg gatcagctgc cggagccggg taaagagatg aactttaaat acaccatcac
     5401 cttcagccgt gatgaagaca aactgcatgc gccagataac gcatgggtgc aacaaacgcg
     5461 tcgttcaacg ggggatgtga agcagtcgaa cctgattcgc cagcctgacg gtactatcgc
     5521 ctttgtggtc gattttaccg gcgcagagat gaaaaaactg ccagaggata ccccggtcac
     5581 agcgcaaacc agcattggtg ataatggtga gatagttgaa agcacggtgc gctataaccc
     5641 ggttaccaaa ggctggcgtc tggtgatgcg tgtgaaagtg aaagatgcca agaaaaccac
     5701 tgaaatgcgt gctgcgctgg tgaatgccga tcagacgttg agtgaaacct ggagctacca
     5761 gttacctgcc aatgaataag acaactgagt acattgacgc aatgcccatc gccgcaagcg
     5821 agaaagcggc attgccgaag actgatatcc gcgccgtaca tcaggcgctg gatgccgaac
     5881 accgcacctg ggcgcgggag gatgattccc cgcaaggctc ggtaaaggcg cgtctggaac
     5941 aagcctggcc agattcactt gctgatggac agttaattaa agacgacgaa gggcgcgatc
     6001 agctgaaggc gatgccagaa gcaaaacgct cctcgatgtt tcccgacccg tggcgtacca
     6061 acccggtagg ccgtttctgg gatcgcctgc gtggacgcga tgtcacgccg cgctatctgg
     6121 ctcgtttgac caaagaagag caggagagcg agcaaaagtg gcgtaccgtc ggtaccatcc
     6181 gccgttacat tctgttgatc ctgacgctcg cgcaaactgt cgtcgcgacc tggtatatga
     6241 agaccattct tccttatcag ggttgggcgc tgattaatcc tatggatatg gttggtcagg
     6301 atttgtgggt ttcctttatg cagcttctgc cttatatgct gcaaaccggt atcctgatcc
     6361 tctttgcggt actgttctgt tgggtgtccg ccggattctg gacggcgtta atgggcttcc
     6421 tgcaactgct tattggtcgc gataaataca gtatatctgc gtcaacagtt ggcgatgaac
     6481 cattaaaccc ggagcatcgc acggcgttga tcatgcctat ctgtaacgaa gacgtgaacc
     6541 gtgtttttgc tggcctgcgt gcaacgtggg aatcagtaaa agccaccggg aatgccaaac
     6601 actttgatgt ctacattctt agtgacagtt ataacccgga tatctgcgtc gcagagcaaa
     6661 aagcctggat ggagcttatc gctgaagtcg gtggcgaagg tcagattttc tatcgccgcc
     6721 gccgtcgccg cgtgaagcgt aaaagcggta atatcgatga cttctgccgt cgctggggca
     6781 gccagtacag ctacatggtg gtgctggatg ctgactcggt aatgaccggt gattgtttgt
     6841 gcgggctggt gcgcctgatg gaagccaacc cgaacgccgg gatcattcag tcgtcgccga
     6901 aagcgtccgg tatggatacg ctgtatgcgc gctgtcagca gttcgcgacc cgcgtgtatg
     6961 ggccactgtt tacagccggt ttgcacttct ggcaacttgg cgagtcgcac tactggggac
     7021 ataacgcgat tatccgcgtg aaaccgttta tcgagcactg cgcactggct ccgctgccgg
     7081 gcgaaggttc ctttgccggt tcaatcctgt cacatgactt tgtagaagcg gcgttgatgc
     7141 gccgtgcagg ttggggggtc tggattgctt acgatctccc gggttcttat gaagaattac
     7201 cgcctaactt gcttgatgag ctaaaacgtg accgccgctg gtgccacggt aacctgatga
     7261 acttccgtct gttcctggtg aagggtatgc acccggttca ccgtgcggtg ttcctgacgg
     7321 gcgtgatgtc ttatctctcc gctccgctgt ggtttatgtt cctcgcgctc tctactgcat
     7381 tgcaggtagt acatgcgttg accgaaccgc aatacttcct gcaaccacgg cagttgttcc
     7441 cggtgtggcc gcagtggcgt cctgagctgg cgattgcact ttttgcttcg actatggtgc
     7501 tgttgttcct gccgaagtta ttgagcattt tgcttatctg gtgcaaagga acgaaagaat
     7561 acggcggctt ctggcgcgtt acattatcgt tgctactgga agtgcttttt tccgtgctgc
     7621 tggctccggt acgcatgctg ttccatacgg tcttcgttgt cagcgcgttc cttggctggg
     7681 aagtggtgtg gaattcaccg cagcgtgatg atgactccac ttcctggggt gaagcgttca
     7741 aacgccacgg ctcacagctg ctgttagggt tagtgtgggc tgttgggatg gcgtggctgg
     7801 atctgcgttt cctgttctgg ctggcaccga ttgtcttctc gttgatcctg tcaccgtttg
     7861 tttcggtgat ttccagccgt gccaccgttg gtctgcgaac caaacactgg aaactgttcc
     7921 tgatcccgga agagtattca ccgccgcagg tgctggttga taccgatcgc ttccttgaga
     7981 tgaatcgtca acgctccctt gatgatggtt ttatgcacgc ggtgtttaac ccgtcattta
     8041 acgctctggc aaccgcaatg gcgaccgcgc gtcaccgcgc cagtaaggtg ctggaaatcg
     8101 cccgtgaccg ccacgttgaa caggcgctta acgagacgcc agagaagctg aatcgcgatc
     8161 gtcgcctggt gctgctaagc gatccggtga cgatggcccg tctgcatttc cgtgtctgga
     8221 attccccgga gagatattct tcatgggtga gttattacga agggataaag ctcaatccac
     8281 tggcattgcg taaaccggat gcggcttcgc aa
//
LOCUS       DAJVTY010000166         8274 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVTY010000166 DAJVTY010000000
VERSION     DAJVTY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.166"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(303..881)
                     /gene="mocA"
                     /locus_tag="OQQ66_003471"
     CDS             complement(303..881)
                     /gene="mocA"
                     /locus_tag="OQQ66_003471"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS1906264.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            984..1754
                     /gene="yqeC"
                     /locus_tag="OQQ66_003472"
     CDS             984..1754
                     /gene="yqeC"
                     /locus_tag="OQQ66_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS1906265.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1802..3427
                     /locus_tag="OQQ66_003473"
     CDS             1802..3427
                     /locus_tag="OQQ66_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS1906266.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3468..4400)
                     /gene="arcC"
                     /locus_tag="OQQ66_003474"
     CDS             complement(3468..4400)
                     /gene="arcC"
                     /locus_tag="OQQ66_003474"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1906267.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4448..5833)
                     /gene="hyuA"
                     /locus_tag="OQQ66_003475"
     CDS             complement(4448..5833)
                     /gene="hyuA"
                     /locus_tag="OQQ66_003475"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCS1906268.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5886..7097)
                     /locus_tag="OQQ66_003476"
     CDS             complement(5886..7097)
                     /locus_tag="OQQ66_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS1906269.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7155..>8274)
                     /gene="dpaL"
                     /locus_tag="OQQ66_003477"
     CDS             complement(7155..>8274)
                     /gene="dpaL"
                     /locus_tag="OQQ66_003477"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS1906270.1"
                     /translation="SQAKLARQFHQKIAGYRPTPLCALDDLANLFGVKKILVKDESKR
                     FGLNAFKMLGGAYAIAQLLCEKYHLDIETLSFEHLKNAIGEKMTFATTTDGNHGRGVA
                     WAAQQLGQNAVIYMPKGSAQERVDAILNLGAECIVTDMNYDDTVRLTMQHAQQHGWEV
                     VQDTAWEGYTKIPTWIMQGYATLADEAVEQMREMGVTPTHVLLQAGVGAMAGGVLGYL
                     VDVYSPQNLHSIIVEPDKADCIYRSGVKGDIVNVGGDMATIMAGLACGEPNPLGWEIL
                     RNCATQFISCQDSVAALGMRVLGNPYGNDPRIISGESGAVGLGVLAAVHYHPQRQSLM
                     EKLALNKDAVVLVISTEGDTDVKHYREVVWEGKHAVAP"
BASE COUNT         1952 a         2015 c         2165 g         2142 t
ORIGIN      
        1 acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga tagagcaaca
       61 atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc ggctcgaaac
      121 ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat cactcctgaa
      181 aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag tgattcctta
      241 tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt taactacgtt
      301 ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt atcaatatct
      361 aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag gagtgcctga
      421 cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt tgacactaaa
      481 atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt tcgtaacgac
      541 cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt gagaaaacaa
      601 tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc ctgcgcataa
      661 tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg cagttcatta
      721 ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt tttgatactt
      781 gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc cattcttgat
      841 gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc attatttata
      901 ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg ggataattta
      961 tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca ttattcattg
     1021 atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg gggaaaacca
     1081 gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg ttaatcacga
     1141 cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt cgtgatcccg
     1201 ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac agctggaaag
     1261 cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg gtgcaacgac
     1321 cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg ttaaaagcgc
     1381 ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct gtgatgggag
     1441 gacatatttt gggcgcgaaa gtgagcacag aaaatgtcca tcgctggtcg cagtttgctg
     1501 atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt gcgctggttc
     1561 gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc tggttcatta
     1621 accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa ccgctgcaac
     1681 aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca atcgcgcgca
     1741 gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca ttgaggttgg
     1801 tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt ttgcgatggc
     1861 gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt tagtgcgcgc
     1921 cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg tgattgaaga
     1981 gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta tggctcgtaa
     2041 cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta tcagcgtcca
     2101 tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat gtcaaccggg cgatagccca
     2161 gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg aaagcctcaa
     2221 tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg gtgcggcagt
     2281 ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata atcaggatcg
     2341 tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc tggcaagtcg
     2401 ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg aagaacatat
     2461 tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc aggagatcgc
     2521 catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg ggctgatgat
     2581 gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg gagatatcgc
     2641 cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc tggaagtgga
     2701 aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg atggcgaaat
     2761 gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga tgaaacttac
     2821 cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg atgaactgaa
     2881 accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc gggcagatat
     2941 ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt gtcatgcggt
     3001 aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt gtgcgcagga
     3061 gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga tccgtgctcc
     3121 tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag agggcgatgt
     3181 gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg tgcgtggctt
     3241 gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg atcctcgtgg
     3301 tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg gcggcgtact
     3361 tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag tgctggaagt
     3421 ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta ctgacaaatc
     3481 accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt aatcagtgct
     3541 tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg cagcatagaa
     3601 cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga caggcgatcg
     3661 agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc agtcagaatg
     3721 atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc tttatcgata
     3781 accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc accaccgcca
     3841 acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt ttcaatgata
     3901 tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc ttctttcaga
     3961 gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga accgatcggc
     4021 ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt aaccagcgtt
     4081 gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt ttgtagatcg
     4141 taaccaatat aaccctggct taaggcaaca cagacagaca tcggcagcat tggcgagtgt
     4201 gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg cggcccgtta
     4261 ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc ctgagaagta
     4321 atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc gcccagggcg
     4381 agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta tagatccctg
     4441 acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga cctcgcccgg
     4501 ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag gttctgacaa
     4561 tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg tggagatggc
     4621 gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca tcggaaccgg
     4681 gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt gcgctggtta
     4741 attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa aacagtaact
     4801 gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg cctttagaaa
     4861 tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg tcaatcgcac
     4921 catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc ggtgggctaa
     4981 gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg agatattgtg
     5041 ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga taatccagac
     5101 ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt tgtgccaggt
     5161 taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag gcatgatagc
     5221 gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata gccgcatcat
     5281 tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat gcctgcaaaa
     5341 cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg ctcagtcctt
     5401 cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg atcacaccgt
     5461 gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc tccagttgat
     5521 ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt gttgtaccgc
     5581 cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg atgccgacat
     5641 caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag ccagtggcat
     5701 caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga acaatcccgc
     5761 tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg ccgtttttga
     5821 tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga ataagtgaag
     5881 agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc ggcacaggtc
     5941 accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc tttacccggg
     6001 ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt agttgagaag
     6061 gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt caccagtgct
     6121 ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc ggttgggtaa
     6181 accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc gttagctttc
     6241 tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc gccccaggtc
     6301 agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct tggggatgtg
     6361 aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata acccagacgt
     6421 tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc gttgtcaccg
     6481 cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat ttccatacga
     6541 ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac aaattccggg
     6601 cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc ttcttcctgc
     6661 acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt aatgatttta
     6721 ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg accaccgatt
     6781 agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat gttgccaatg
     6841 ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc gatataaccg
     6901 agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc catctcttct
     6961 ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc aaccatgtcg
     7021 cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat cagtttgaat
     7081 ggaatattct tagccattat ttttctccag attttttact ccccgttttg cagggagtag
     7141 ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac ttcgcggtag
     7201 tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc tttgttcagc
     7261 gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc gagaacgccc
     7321 aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta cggattaccc
     7381 agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg ggtggcgcag
     7441 ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc cgccatgatg
     7501 gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg ataaatacag
     7561 tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta gacgtcgacc
     7621 agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag aacgtgcgtc
     7681 ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt tgcgtagcct
     7741 tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg taccacttcc
     7801 cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata gttcatatcc
     7861 gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg agcagaacct
     7921 ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc cacaccgcgc
     7981 ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc atttttcagg
     8041 tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa ttgagcgatg
     8101 gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga ttcgtctttg
     8161 acgagaattt tcttcacacc aaaaaggttt gcgagatcgt ccagcgcaca aagcggtgtt
     8221 gggcgataac cagctatttt ctggtggaac tggcgcgcca gtttggcctg gctt
//
LOCUS       DAJVTY010000167         8141 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVTY010000167 DAJVTY010000000
VERSION     DAJVTY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.167"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..391)
                     /locus_tag="OQQ66_003478"
                     /pseudo
     CDS             complement(1..391)
                     /locus_tag="OQQ66_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(577..3885)
                     /gene="icsA"
                     /locus_tag="OQQ66_003479"
                     /gene_synonym="virG"
     CDS             complement(577..3885)
                     /gene="icsA"
                     /locus_tag="OQQ66_003479"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCS1906271.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGFTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4414..5616
                     /gene="virA"
                     /locus_tag="OQQ66_003480"
     CDS             4414..5616
                     /gene="virA"
                     /locus_tag="OQQ66_003480"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCS1906272.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5795..6482
                     /locus_tag="OQQ66_003481"
                     /pseudo
     CDS             5795..6482
                     /locus_tag="OQQ66_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6075..6191
                     /locus_tag="OQQ66_003481"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6484..6850
                     /locus_tag="OQQ66_003482"
                     /pseudo
     CDS             6484..6850
                     /locus_tag="OQQ66_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6778..7251
                     /locus_tag="OQQ66_003483"
                     /pseudo
     CDS             6778..7251
                     /locus_tag="OQQ66_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT         2397 a         1867 c         1625 g         2252 t
ORIGIN      
        1 aggctgtacg cttccccatg tgatttgcct cgccacttca ccgggctcag gaccccgtag
       61 gatcttcacc gaaaaaaatg catcaccagt tgctttatta tttgttttag gtagacctgt
      121 tactaattta tacctactct gaccaagtct ggataagaaa ggcacacctg cacatttcta
      181 cttggaaatg ttttctttta ccattttgct caaaaatttc aacatccaat attcctcgta
      241 cagaactact caagtcctga atagaaaaag ttccagctgg tacagtagag tcatatattg
      301 ctcgaccttg ttgagataca acaacacgtg cattagtttc tgcaatacca gtaatctgag
      361 gcgcataacc acgaatattt ggcggtagca ttcgttcatc gctttctaat gcaattcctg
      421 tgtaacgcca agcaacagcg cattctggcg catgatctgg taaacacgtt tggcattgat
      481 cgcaggcata ccatcatgtg cacaaaaacc caccgagctt ttacacggtg ggtcccagag
      541 aaatgcagga catcaacacg ccctgcattt ttattatcag aaggtatatt tcacacccaa
      601 aataccttgg gtgtctctgt aactgttatt acccaattgc tgagatacgt ttccccagat
      661 gcttaggtta tctgcaagac gcccccccac accagttttg agttcaatca cattacggtt
      721 gcctatctgg tgatttgaca tgccattcat aattacacca tattgctttg agctccattt
      781 ccaattcact tccaaaaatg gttcgaactt acgaagagtc tccttatcga tacaactatg
      841 actaattaag gatgtacgca atcccaattt tgcttgaata ttattgaccc cagaggatgt
      901 gaccatagtg ccattgtgtt caatatagtc gtttgctttt accccatgcc tagttatctg
      961 gacttgtgga ttgagataca atgcgttgtc aatattatta tgagtagtcc agcgtatggt
     1021 tgggtagata tagccagctt ccaaagctcc tgttattcct ttgctggaat atttctcacc
     1081 agtaaaccca tctcctttga ctgtattatt aaaccaaccg tactgcaacc aagcatccat
     1141 atatagacct gttcgttctt tttcatcctg aaaccaagaa ctgtacaaac cggcagtata
     1201 accgctcaca gttcctcgtg aactataact agtatactta ttatgagttt tgctatcctg
     1261 atatccataa gctcccataa tacccacatg caagccatca tgatcagtga agtttgtctt
     1321 caaaatatct ccaccaatct gataaaccat cctgttagct gtggttttta attgtctatc
     1381 agctaaatta tgctcattat gtccaccaac agtacgaatc cacatggtgg tttcgctgag
     1441 ctgacctgta gtaggatcaa caatagtgct accgtgtcgg tcatagagag aaggactaaa
     1501 catcgtgttc attgctatca ggttagcaat ataactgcca gcttcagggc gccatgtgtg
     1561 aatactacct tcacccaaat taagtgaaga tacaagctgt gaactagctc ggcgactact
     1621 catttgagta gactcttgat tgctcatctg ttttgattct tgattatcca tctgactagt
     1681 tagataccac ttattggtat tcagtccaga tacagtgccc tgtttcaggc gatagtcata
     1741 actaccagca acaatacggc ctttctgaat aaaagcattc ttatcagaag aatctgtcga
     1801 aataatttga acaccttcaa gagttttatc tccagagcct ccctcatttg tcactgcaac
     1861 ataggtaata ccagacgcat ggcctgtaat ttccagccgg tcagttttac tgttgtcatt
     1921 gccgagaacc gcgcccaaag ataatattcc gccctttcca tgccaattac ctttctgaac
     1981 ataggtctga ccgacatttg acgaactatt attgagaata acatgaccgt ttttctccag
     2041 agtcatgtta cctgtgacaa tgacggggtc aggcaaaaaa ggggcattaa tattattgat
     2101 tagcacagtc ccactattta ataaagtgtt aacagtttgg ttcatgcctg aaaaattcaa
     2161 ggtagcacct ttattaacaa taacaccagc ggtacgtgtc atagcttcaa ctgtccccat
     2221 ttttagaatg cctgcattga tgttggtgaa accagagtag gtattttttt ccgccaaaat
     2281 gagagttccc tcccctgatt ttgttaggga ttttccgtcc cagctattta tattatgatg
     2341 gctgagatta tctgccagaa tagtactaac agtaagttca gcgttttctt tcagattgaa
     2401 ctctccatga ctgtcgccat ctgtatcatt ccagctcaaa ccgtagatca atttattctg
     2461 gtcactgata tagctttttg tatagtcaat agcaccagta gtgagtaacg gattgatgat
     2521 agatatatta gaaacactga aacctgttat ccctttatct gcacttagca gtttatagtc
     2581 cttctgcgcc cagttattta taatagcttc cacttttaca ttgttgccaa atgacatatt
     2641 tccagaagct tccagactac tgattccagc taaatttaaa gaactgttct caccaaaaga
     2701 aacgtcacct gaaacagtga atttgttacc tgatagtgaa acagttgtac aatcaccagc
     2761 acataaatca ccttcaatag ttggtgtggt cccagtgtaa tcattgttga taattttcag
     2821 aatggatgaa ttgttaacct gtacatcacc agtaatgaca gaaccttcat ggagtattaa
     2881 tgaattgttt ccaccagtga tgtggatagc attacctgct acattttgtg ttccatcatc
     2941 ttctttacct gaaattatat atccgctatt agcaatggtc atattgctac catttatagc
     3001 atccccccaa tgtccaccat taccgcctaa aatagctcca ttgttgatta tagacaggtt
     3061 atctcctgtg atagcattac caccgtagcc atcataacca taaccgtaag cagcacctcc
     3121 gttgcctcca gcgaacgtgc ccttattgat tacagagagg ctgcttcctg tgatggcatc
     3181 acctccatta ccgccatcac cctcaccata attgttacct ccattaccac cggaaatagt
     3241 ccctttgttg attatgggta gattgactcc tgtgacggca tcacctccat tccccccatc
     3301 actatcacta taactatcgc caccatgtcc acctgaaata atttctccat ttattataaa
     3361 cagattgtct cctgtaacag cctcaccacc atcaccgtta tggtcagcac cgctaccgcc
     3421 gctaccacca agaatcatgc cttgattgat tatagacaag tcagatccgg taatagagtc
     3481 accaccatta ccgccacaac tattaccatc attattatca ccagcaccac catgacctgc
     3541 gctaatcata atatcagagt taatccgtag cttagttcta gatgcatgag aggggttgga
     3601 agaagttata taccacgcat ccattccatc ttcaccagct cccaagggag aaagtccatc
     3661 aacaggtgtt aataattttt cataattgtc ctctgaaaaa tgaagttctt gagtacccga
     3721 aagaggaata gcaaaagcta ttggcccccc gagcaacagg gatgcaccaa caacaaaagg
     3781 agaaaaagac aatttgcatg ttttttcctt taccgtaggt aattctccgg cccccccctg
     3841 tgaacattgg gtcatattac aaaaaaattt gtgaatttga ttcatgcact atattatcag
     3901 taagtggttg ataaacccct aaaaagattc cgttttatcc ggaataaagg gacgatatat
     3961 gcaaaaacat attaaacaaa gcctcaacca aaacatgaga gctgtgttat atatcaatgc
     4021 aacgggagaa atttacctgt gtcttgcttt gaaaaaaaga gggggaaagc tatatgttcg
     4081 ataataaatt aataatttta ctatgatagt ctgcttcaga ccgcaacttt tcagttcttt
     4141 ctgtacgctt gcccaaagtt taaccacttt gtttttctat acacccaatg gttctatctg
     4201 ccccttgatg ttaatcccca aagccaataa catgaatgta ttcatatcac aactttccta
     4261 ccagactctc caatgcaata actgcataat tgtttttagt ggaaatgtat cgttttctta
     4321 aagagaagaa taacattcca ttttattatg ttcccaattt aattatatta atatatattg
     4381 gcatcaatag gaaaatacat caggagaaat caaatgcaga catcaaacat aactaaccat
     4441 gaaagaaatg actcatcatg gatgtcaact gtcaaatcaa ctacagaagt aagttggaat
     4501 aaattgagtt tttgtgacgt tttactgaaa ataataactt tcggaatata ttctccacac
     4561 gaaactctag ccgaaaaata tagcgaaaaa aaactaatgg atagtttctc accatcacta
     4621 tcacaagata aaatggacgg agagtttgcc cacgcaaaca tagatggcat ttcaatacgg
     4681 ctatgcctta acaaaggcat atgttcggtt ttttatcttg atggagataa aatacaatcc
     4741 actcaacttt catcaaaaga atataataac ttgttaagtt cactcccccc caaacaattc
     4801 aacttaggaa aagtacatac tattacagct ccggtatcag ggaattttaa aactcacaag
     4861 cctgcaccag aagttattga aacagccatt aattgctgta catcgataat accaaacgat
     4921 gattacttcc ccgttaaaga cactgatttt aatagtgttt ggcatgatat ttacagagat
     4981 attcgagcat ctgacagcaa tagtacaaaa atatatttca acaatattga aataccacta
     5041 aaattaattg cagatctaat taatgagtta ggaattaatg aattcattga tagcaaaaaa
     5101 gagcttcaaa tgctaagtta caaccaatta aataaaataa tcaattcaaa cttcccccaa
     5161 caagacctat gttttcaaac agaaaaactt ttgtttactt cactctttca agaccctgca
     5221 tttatatccg cactaacatc tgcattctgg caatctcttc acatcacgtc ttcctctgtg
     5281 gaacacatat atgcccaaat tatgtccgaa aacatagaaa acaggcttaa tttcatgcct
     5341 gaacaacgag ttattaacaa ttgtggacat attataaaaa tcaacgcagt tgtccctaaa
     5401 aacgacactg caatctctgc ctctgggggg agggcttacg aagttagctc atcaatatta
     5461 ccatcgcaca taacatgtaa tggtgttggt attaataaaa tagaaacaag ctatctcgtt
     5521 catgctggaa cacttccaag cagtgaaggg cttcgcaatg ctatccctcc agaaagtcgt
     5581 caagtatcat ttgccatcat atctcctgat gtttaagcca atattatatt ggctgccaga
     5641 ctgtaaaaaa acgttcttca gaacagctct ataaactatt aaaccaaagc taaaaggatg
     5701 gggaccaata gaatctcagc ccatcctttt tatatcatta tgaaccgccc cggaaatcct
     5761 ggagactaaa ctccctgaga aagaggtaaa caggatgact aaaaatacac gtttttcccc
     5821 tgaagtccgt caacgggcag ttcgtatggt tctggaaagt cagggcgaat atgactcaca
     5881 atgggcagcg atttgttcca ttgctccaaa gattggctgt atactggaga ctctgcgtgt
     5941 ctgggtacgc cagcatgagc gggataccgg gggcggtgag gtgggctcac caccgctgaa
     6001 cgtcagcgtc tgaaagagcc ggaacgtgaa aatcgtgaac tgcgccgcag taacaatatc
     6061 cttcgcctgg cttccgctta ttttgcaaag gcggagttcg accgcctctg gaaaaaataa
     6121 tgccactgct ggataagctg cgtgagcagt acggggtcgg accggtatgc agtgaactgc
     6181 atattgcccc gtcaacgtat taccactgtc agcaacagcg acatcatcat gataaacgca
     6241 gtgcccgtgc gcagcgcgat gactggctga agaaagagat actgcgcgta tacgatgaga
     6301 atcatcaggt atacgctgtg cgtaaagtca ggcatcagtt gttacgggaa ggtatcagag
     6361 tggccagatg cactgtggca cgtctcatgg cggttatggg gcttgccggt gttctccggg
     6421 gtaaaaaggt ccacactacc gtcagccgga aagccgttgc cgcaggcgac cgcgtaaacc
     6481 gtcatcaagg caatgtgccg cgtactccgg gtggcccgca gcggctggta tacgtggtgt
     6541 cagcggcgga caaggataaa cacacgtcag cagttccgtc agcactgcga cagcgttgtc
     6601 cgcaaggctt ttacccagtc cagcgttacg gtgccccacg cctgacggat gaactgtgtg
     6661 ctctggttac cactttaacg taaaaaccgt ggcagccagt ctgcgccgtc aggaactgag
     6721 cgcaaaagcc tcccagaagt tcagcccgat cagttaccgt gcacacggcc tgcctgtatc
     6781 agaaaatctg ctgacgcagg atttttacgc cagtggcccg aaccagaagt gggcgggaga
     6841 catcacgtac tattacagct caccgactgc ggggaaacac ggggcgccgg gttattgaag
     6901 gtgacctgtc cagctacttc gacacagtac atcaccggct gctgatgaaa gccgtctgcc
     6961 gcaggatcag tgatgcacgt tttatgagac tgctgtggaa aacgccgtgt tgatgttggt
     7021 ctcttccggg cggtcagtga aagtatgcca cagggcggtg tgatctcgcc gttgctgtcg
     7081 aacatcatac tgaatgagtt cgaccagtat ctgaataaac gctacctgag tggaaaagcc
     7141 agaaaggatc gctggtactg gaatcacagt atccagcgag gccgaagtac tgctgtcaaa
     7201 gaaaactggc aatggaaacc tgccgttgct tactgttgct atgccgatga ctgtgtgcca
     7261 cgaaggcgcg tcctggggac gtgagtccct gtgcagccat gcggcacaag agatgaggga
     7321 aggcccctcg gtgtcggcgt gcaggcgcag gcaccaaacc atccactgct gctgcattta
     7381 agagatgtgg tggtgagcga gatgggaaaa ggtcggacgt gagccgatca ggtatggttc
     7441 gaagagacga acgaaagtga accgctgatg atgtgtcgaa agcatataga tgatgtcaaa
     7501 acaatggtgg agtcgttcct gtgggatatg tttactgcct gcgcggcatc cggcataaaa
     7561 gcggcgtgaa gtcgattcag gctcttgtgg ggaacgtgga cctgtcaccc cgatgtgaag
     7621 ggagaagcgc aagtggaagc cccatgagcg ccagagtacc aatgcggggt acagggacgg
     7681 agcaccaagc gattgcgcag taaacatgct tgacaactta attggggccc cctcttcctt
     7741 ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat gaagtatatt
     7801 taatatactt tacaaaaagt ttgtattagt ggaataagga caatagttta tccaaactgc
     7861 caagcccctt tattataagt aagatctggt actaaataag cgttctaaac cgacccgagt
     7921 aggcggcctg acggctgcgg cttgtcatgg cctgtgatta ccgttataaa aacagacaat
     7981 atcattgtct ttcaggtagt tatatggccc gttcagctaa accacgcaaa cgaaaacctg
     8041 cacctcaaag aagcaaactc ctccactatg tcgtaaaact tcacgaagat gatttttttt
     8101 gacgaagaag aagctgaggt tctgcgcttt gataattttg a
//
LOCUS       DAJVTY010000168         8138 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVTY010000168 DAJVTY010000000
VERSION     DAJVTY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.168"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..262
                     /locus_tag="OQQ66_003484"
     CDS             <1..262
                     /locus_tag="OQQ66_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1906273.1"
                     /translation="AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFR
                     FYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(401..1030)
                     /locus_tag="OQQ66_003485"
     CDS             complement(401..1030)
                     /locus_tag="OQQ66_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1906274.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1153..2799)
                     /gene="fumB"
                     /locus_tag="OQQ66_003486"
     CDS             complement(1153..2799)
                     /gene="fumB"
                     /locus_tag="OQQ66_003486"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1906275.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2877..4217)
                     /gene="dcuB"
                     /locus_tag="OQQ66_003487"
     CDS             complement(2877..4217)
                     /gene="dcuB"
                     /locus_tag="OQQ66_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1906276.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4788..5507)
                     /gene="dcuR"
                     /locus_tag="OQQ66_003488"
     CDS             complement(4788..5507)
                     /gene="dcuR"
                     /locus_tag="OQQ66_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1906277.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5504..7135)
                     /gene="dcuS"
                     /locus_tag="OQQ66_003489"
     CDS             complement(5504..7135)
                     /gene="dcuS"
                     /locus_tag="OQQ66_003489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1906278.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7316..7546
                     /locus_tag="OQQ66_003490"
     CDS             7316..7546
                     /locus_tag="OQQ66_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS1906279.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7558..7830
                     /locus_tag="OQQ66_003491"
     CDS             7558..7830
                     /locus_tag="OQQ66_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1906280.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8057..>8138
                     /gene="ghoS"
                     /locus_tag="OQQ66_003492"
     CDS             8057..>8138
                     /gene="ghoS"
                     /locus_tag="OQQ66_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1906281.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRL"
BASE COUNT         2018 a         1968 c         2083 g         2069 t
ORIGIN      
        1 tgcggttgtg ttagggatga ttggctatgt accgaatgtt gaacagtcta cgcaagccct
       61 attaggtatg cagtttatta tgattgctct accaactctg tttttcatgg taacgctgat
      121 tctctacttc cgtttctatc gcctcaatgg tgacacgctg cgcaggatcc agatccatct
      181 gctggataaa tatcgcaaag taccgcccga gcctgttcat gctgatattc cggtcggtgc
      241 agtgagtgat gtgaaagcct gacgtggaca atagctaacg aaaatggcct gatgcgatat
      301 gtttatcagg ccacgtctta atgtaatgct ttggatttgc aaattttgta gaccggataa
      361 ggaattcacg ctgcatccgg catcaacaaa gcgcacgttg ttatccggtt atcaagccaa
      421 agcgccgtag ctggcggcaa tgaaagcgag cgaggaatat caccgtcgtt aatgcgccaa
      481 gcagcgcgca gaacatatcg gattgcgtgt cccactggtc gccctgggtg ccgagaaaat
      541 catccgctcc ctgacccatt gccagcgccg cccaccactc gattaattca tacattgcgc
      601 ttatcgccag cgccacgcag cagactagga acgccaccat tttacgtccg cgcacgtaca
      661 tcccgcgaac gagaatttct cgtgccacca gtgcaggcac cagcccctgg aaaaagtgcc
      721 ccagcttgtc atacggatta cggctcaacc ccagccattc ctgcacctca aaaccaacgg
      781 ggactttcgc gtaggtgtat tgtccgccga ccatcaggat gatggcgtga aggaaaatga
      841 gcgtatagag cagcggcgtt aacggataac gtctggcggt ggcaagcagt agcggcacga
      901 caataatcac cggtgtcact tccatcagcc aggtgagttt gtcatgggcc gaaatgccgg
      961 tataaatcag gattaatgtt agcgccagtg cgccggtgtt aagaattaac ggcttgagtg
     1021 tgcgggtcat ggtgagttcg caaatcaggg aaaatactga ctattcacca gcaagcgtga
     1081 aattacaatc gcaaaagagg tggcccaggg ggatcacctg gcagcatgct gccaggcgct
     1141 gggccgaaga ggttacttag tgcagttcgc gcactgtttg ttgacgattt gctggaagaa
     1201 gtcgttacct ttgtcatcga ccaagataaa cgccgggaaa tcttctactt cgattttcca
     1261 gatagcttcc atacccagct ccggataagc gacgcattcc agatgcttga tgctctgctg
     1321 cgccagtacc gccgccggac cgccgatgct accgaggtag aagccgccgt gtttatgaca
     1381 cgtgtcggta acctgctgac tgcggttacc tttcgccagc atgatcatgc tgccgccgtg
     1441 ggattgcagc agatccacgt aggagtccat acggcctgcg gtggttgggc caagtgaacc
     1501 tgatggataa ccggcagggg ttttcgccgg acccgcgtag tagatcgggt gatctttgat
     1561 gtactgcgga agttctttac cggcgtcaat cagctctttc agcttggcgt gtgcaatatc
     1621 tcggcccacg ataatggtgc cggtgagcga caaacgagtg gataccgggt attgcgaaag
     1681 ctgggcgagg atctctttca tcgggcggtt aaggtcaact ttcaccgctt cgccttcacc
     1741 ggcctggcgc agttcttgtg gaatgtactg gcctgggttg tgttccagtt tttcgatcca
     1801 gataccttcg cggttgattt tcgctttaat gttacggtca gcggagcagg agacgcccat
     1861 gccgaccggg caggatgcgc cgtgacgtgg cagacggata acgcgaatgt cgtgcgcgaa
     1921 gtatttaccg ccaaactgtg cgccaagacc gagtttctgg gcctcttcca gcagttcctg
     1981 ttccagctgg acatcgcgga acgcctgacc atgttcgttc ccttccgtcg gcagttcatc
     2041 gtaatagtga gcgcttgcta acttgacggt tttcaggttg gtttccgcag acgtaccgcc
     2101 aatcacaaac gcgatatggt acggcgggca ggctgcagta ccgagggtac gcattttctc
     2161 gacgaggaag tttttcagtt tgccgggagt cagcagggct ttggtttcct ggtagagata
     2221 cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg aatttgtact catcgccatc
     2281 taccgcgtac aggtcgattt gcgcaggcag gttagtgccg gtgttgacct ctttgtacat
     2341 gtccagcgcc gcattctgtg aatagcgcag gttatcttcg atataggtgt tatagacgcc
     2401 tttcgacagc gcttcttcat caccgccgcc ggtccacacg cgctggcctt ttttaccgac
     2461 gatgatcgcg atgccggtat cctggcaggt cggcagcacg cctttggcgg cgatttcgga
     2521 atttcttaag aattgcagcg ccacgtactt gtcgttttcg ctggcttctg gatcgtgaag
     2581 aatagccgca acctgtttct ggtgtgccgg gcggagcata aaagaagcgt cgtgaaaggc
     2641 ttgctgcgcc agcagggtca gggcttctgg ttccactttc aggatggttt cgccgtcgaa
     2701 gtcggcaacg ctaacgtaat cggaagtgag tagatagtat tcggtattgt ctttccccat
     2761 cgggaaaggt gcctggtaga taaagggttt gtttgacata gcttccagcc tgtaactctg
     2821 tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc ggcacgcaaa gtgcatttat
     2881 aagaacccgt acatcgcggc gaagatccag ccgaagacgc acgatacgct cacaccaatc
     2941 aaccccggca gaataaagct gtggttgatg acgaagcgac cgatgtgggt ggtgccggaa
     3001 cggtcaaact gaatcgctgc cagatcgctc ggataagtcg gcaggatgta ataaccgtag
     3061 caagccggtg ctgaagccac gatgtatgcc ggatcaacgc cgatcgccaa cgcgaccgga
     3121 acaatcgccg ccagcgccgc agcctgagag tttacaaact tggaaaccag cagcagaaca
     3181 atggcatagg cccacggata ctctttcacc atttcaccca gtacgccctg aatttcagac
     3241 atatgcgcac cgaacatggt ttctgccatc catgcgatac cgtacaccgc cacgatggcg
     3301 atcataccgg aacggaagac ttcgtttttt gagatagacg cgggattggt tttggtcagg
     3361 ataataatca gcgccccggt cagcagcata aacatctgaa taaccagtac catcgacagc
     3421 ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac caagaagggc gactacagcg
     3481 attgccccga ggaaaatcca cattgccagc cagttgcttt tcggcagttt tttatccagc
     3541 agcgtcgcgg tatcaccgta aacatactca cggttttccg gtacggagat gaatttctgg
     3601 aactcttcgt ctttatccag atctttacca cggaaccagc tgaagatacc gatcgccagg
     3661 ataccgatta acgtcgatgg aatggtgatt gccagcaggt cgaggaactc aagatggcga
     3721 ccatcaaagg tgacattacc cagcatcgca accagagaca cgaccgcaac cgacaccgga
     3781 ctggcgataa tccccatctg tgcaccgata gaacttgccg ccatcggacg ttccggacgg
     3841 atgttgttct taatggcgac gtcgtagatg atcggcagaa tggtgtaaac cacatgaccc
     3901 gtaccgcaaa gaatggtcag cgtacaggtc acaaacggcg cgacaattga gacatatttc
     3961 gggttgcggc gcagcaactt ctcggcaatt tgcagcatga catcaagacc gcccgaagct
     4021 tgcaaggtcg ccgatgccgc caccaccgca atgataacca gcatgacatc aaccggtggt
     4081 ttacctggct gaaggtggaa gacgaagacc agaatgacca gaccgatacc gcctaataaa
     4141 cccagcgcga taccaccctt tctggcacca taaaacagac atatcagtat tatgataagt
     4201 tggatagtaa ataacatgtg tgaaccctcg cgataatcct atttaaattt ttgctgaata
     4261 gatcacagtc acgttctgtt ttgtatgaac tgttttcaga gcctgactaa atatccgttt
     4321 ggtcgttacc ggctttagca aatacctcac agtaaatatt ggctgattaa agcagcggca
     4381 aatttatgca ctgtgataag cggctttttc agagagaaaa actctctggc gatgggttta
     4441 tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt atcttattga tatttatcgg
     4501 tctatttttt gttctcaggg cagatatcat taggtttaaa gattaatctt atctacccat
     4561 ttgtgggtga aaatacacat aatgcgggtg acataatagt taattaactt ttgttagcgt
     4621 tttgaaatta aaaacaccgt tcacctgaag agatattaat ttttagcgat gatggaggga
     4681 taattatatt tgatctggca caagttttac tgatgaagga tgtaacttgt gccaggggta
     4741 ttttgcatta cggtaataat tattacttac agataaccag cgatccgtta ttggcaatat
     4801 tgtttcagta gtgagtagtg ttctgcctga atacggtaac ggtaaaccgg acgccccgtg
     4861 acgccataat ggatactggt gaacaagatg tggcagttga ccagccagat gaggtattta
     4921 cggcaggaaa cacgcgagat gttaacctcg ttagccagct cgtcggttga aaactcatag
     4981 tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg tctgcggcgt taagcctttt
     5041 ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt ggattagctg atcaagctcg
     5101 gcctggtcat aatactgatg tttttccagc gccattttct tttgccgcca gccggtgagc
     5161 gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt aatccacgac accgtaatgc
     5221 agcgaatctt taatggttgc cgcatcggct gcggaggaga tgacaatcac atcacttttg
     5281 caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt tctctttttg catatagata
     5341 tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga taatctcttt ggctttctcc
     5401 agcgtcgagg ctgttccaca gcattgaaag cctgggattt gtgctacgta tcggcgattc
     5461 agctccgcga ccattgcgtc gtcatcgata attaatacat tgctcatctg tttgacctct
     5521 ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat cccgggttcc gattccacgg
     5581 cgatgctgcc gccgagattt tctacctgtt gtttgacaag tgctaaaccg acgcctcgct
     5641 cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc gattttgtcg ggtgcgatcc
     5701 ccggtccatc atcgttaact tcgcagtgca gccagccgtg acggtagtgc aatgttacgc
     5761 taatttcgcc accgggttcc ggccctaatg cttccagcgc gttttctatc agatttccca
     5821 atgtggtaat cagcgtcgcg acctggtcct cactgccgct gtctggcagc aggctttcac
     5881 tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt aatcttgctg attaaaaaac
     5941 cagcgataac cggagatttg atcttaccca gcagagagcc aatctcttcc ttatagttat
     6001 tggctgtttt gagactgtaa tcttccaact gcttataact cttcagatgc aataatccga
     6061 gaatcacatg caatttattc ataaattcgt gggatcgttc acgaagtgca tcagcatagt
     6121 tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc agttttgtcc ctgaaggttg
     6181 aaatggcacc gatgataacg ccattactgc gcaccggaac ggtgttgatc agtaatagcc
     6241 ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc gtcgcgtaac acttccgaga
     6301 catctaccac ctgtgaccat gagtggctta gcgtcgacag tttctcatcg tcctgcgact
     6361 tacggtaatt cagcaattct tgtgcggcat cgttgatcag cgtgacctcg ccgcgatcgt
     6421 ccacggcaac gacgccttct ttgatagact gcaacatggc ctggcgctgc tcaaacagcg
     6481 tggagatttc gtagggttcc aggccgaaaa ggattttttt cagtacctta accagaatgc
     6541 aggtgccaat cagtccgacc agcatgccaa ataataccga ccagataatg ctccagcgac
     6601 tgtcattgat ttgttgggtc acgcggctta actcaaggcc gctcaccacc acgccaattt
     6661 gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa agcctgcgcc agaaaaccgc
     6721 gattgatagc gacattttct ttgccattca gcgctttaag gatgtcatca cctttaaatg
     6781 gctgaccaat acgctgggct tcaggatgcg agtagcgaag actttgcata tccgtaacga
     6841 caataaacag cagatcgttg cgtttgcgca cggcttccgc gatggcctgg attccgctct
     6901 cctgcggttt tttctgcaag ccctgacgga tttccggcga gtcggcgagg gtacgcgcca
     6961 ctgccagtgc cttgttggcc agcccgtctc gtgtcatatc actgatttgc gagaagtaaa
     7021 tcagatgcac caccaatagc accgagaact gtaccgcgct gaccattaag atcactgtgg
     7081 tactcaattt catcggacgt ttgagtaaca tgcggtaggg caatgaatgt ctcatcagct
     7141 tccttgtgtg acaaatttct taagcattat ctctgatgag gcgggtaatt caaagggcgt
     7201 aagaatgatt ggctatatag gggaagagac tctggcaacg gaaactgcca gtgctgtatg
     7261 aagattccag ggctatgctt atagcgataa tcatactgat gagagaggga aagtcatgga
     7321 tcaggcgcta ctggacgagg gttatcgctg ttataccggc gaaaagatcg atgtctattt
     7381 caacactgcg atatgtcagc attctggcaa ttgcgtacgt ggcaacggca agttatttaa
     7441 tctcaaacga aagccgtgga tcatgccgga tgaagtcgac gtcgtcactg tggttaaagt
     7501 gattgatacg tgcccgagcg gcgcgctgaa ataccgtcat aaataagcga gggtaaaatg
     7561 gaaatacgcg aaggccacaa taaattttac attaatgacg aacagggcaa gcaaatcgct
     7621 gaaattgtct ttgtgccgac cggagagaat ttagcgatta tcgaacatac cgatgtcgat
     7681 gaaagcctga aagggcaggg gattggtaaa cagctggttg cgaaagtcgt ggaaaaaatg
     7741 cgtcgggaaa aacgaaaaat tatcccacta tgcccatttg cgaaacacga atttgataaa
     7801 acgcgggagt atgatgatat tcgcagttga tgggagagta cagagtcacg atatttttca
     7861 tgctctccgc ggagtgatgc aggagagcat ctgaagggta gggggatgca caaagaatgg
     7921 gcagagagcg cgtttttttg tcccacgtca tcccctttac tgagcaaaaa aaagaatatc
     7981 tcctatatga gaatcatcaa tcggggttaa taagttttgc gtccccagag cattcaatat
     8041 tgataggagt catattatgg aaggtaaaaa caagttcaat acttatgttg tttcttttga
     8101 ttatccatca tcttattcct cagtgttctt aaggttaa
//
LOCUS       DAJVTY010000169         8104 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVTY010000169 DAJVTY010000000
VERSION     DAJVTY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.169"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(132..863)
                     /gene="dnaQ"
                     /locus_tag="OQQ66_003493"
     CDS             complement(132..863)
                     /gene="dnaQ"
                     /locus_tag="OQQ66_003493"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1906282.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            928..1395
                     /gene="rnhA"
                     /locus_tag="OQQ66_003494"
     CDS             928..1395
                     /gene="rnhA"
                     /locus_tag="OQQ66_003494"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1906283.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1392..2114)
                     /locus_tag="OQQ66_003495"
     CDS             complement(1392..2114)
                     /locus_tag="OQQ66_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1906284.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2148..2903
                     /gene="gloB"
                     /locus_tag="OQQ66_003496"
     CDS             2148..2903
                     /gene="gloB"
                     /locus_tag="OQQ66_003496"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1906285.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2975..4333
                     /gene="mltD"
                     /locus_tag="OQQ66_003497"
     CDS             2975..4333
                     /gene="mltD"
                     /locus_tag="OQQ66_003497"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1906286.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4382..4978)
                     /locus_tag="OQQ66_003498"
                     /pseudo
     CDS             complement(4382..4978)
                     /locus_tag="OQQ66_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5009..5809)
                     /locus_tag="OQQ66_003499"
     CDS             complement(5009..5809)
                     /locus_tag="OQQ66_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1906287.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPLGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6050..6964
                     /locus_tag="OQQ66_003500"
     CDS             6050..6964
                     /locus_tag="OQQ66_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1906288.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(6961..7754)
                     /gene="dkgB"
                     /locus_tag="OQQ66_003501"
                     /pseudo
     CDS             complement(6961..7754)
                     /gene="dkgB"
                     /locus_tag="OQQ66_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
     gene            complement(7916..7992)
                     /locus_tag="OQQ66_003502"
     tRNA            complement(7916..7992)
                     /locus_tag="OQQ66_003502"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7956..7958),aa:Asp,seq:gtc)
BASE COUNT         2147 a         1963 c         1970 g         2024 t
ORIGIN      
        1 cgttgtgttc cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt
       61 ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca agccgctatt tttagcgcct
      121 tttacaggta tttatgcccg ccagaggcag cttccgccct tcttctccac cagatcaaga
      181 cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc
      241 tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc tccttccatc
      301 gcaaaagcca tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgt
      361 gcatcgagta atgccccgtg tagcgttcgt ttactgttat ctatttcgta gcgggcacat
      421 aacgcatcga ggctgttgcg cttaccagga aacattttcc tcgccaccgc aaggctatcg
      481 gtgaccttac agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag
      541 tccataaagc cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag
      601 tccatgaact catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca
      661 ataccatgta cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg
      721 aagttattgc ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg
      781 tggccttcat agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc
      841 tggcgtgtaa ttgcagtgct catagcggtc atttatgtca gacttatcgt tttacagttc
      901 gattcaatta caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt
      961 tcgtgtctgg gcaatccagg acctgggggt tacggcgcta ttttacgcta tcgcggacgc
     1021 gagaaaacct ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc
     1081 gctattgtcg cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc
     1141 cagtatgtcc gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa
     1201 accgcagaca aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcccta
     1261 gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa
     1321 cgctgtgatg aactggctcg tgctgcggca atgaatccca cactggaaga tacgggctac
     1381 caagtcgaag tttaagcttg tggtttacga cattgccggg tggctccaac cgcttggcga
     1441 attcgtggct tgtttttact ctgtttcatc gggtttagcg ttaaaggaat agtccgtttc
     1501 cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagttttcct
     1561 ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt gcagcacttc aaaattcaac
     1621 aaagagagcc agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt
     1681 tttcgcaata ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta
     1741 atcaccagcc agccatcatc aatcaatacc cgatcggttt cacgcaataa acgatgcgga
     1801 tcggtgcacc acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa
     1861 ggaagatgaa gtggatccgc ctgcacctgg acgggcattc cttgcgcaga aacattcact
     1921 tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc
     1981 agatgaaaac catacatttt agtgaaccac gggttgagct ggcgctccag cgccttgcga
     2041 taaagctctc cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact
     2101 cttgccggtt tcatcacaac cttccgtttc acactaagag gtaatctatg aatcttaaca
     2161 gtattcccgc ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc
     2221 tgattgtcga tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc
     2281 aaccggaggc catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac
     2341 tggtggaaaa gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa
     2401 caacacaggt agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa
     2461 ttgccacacc gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt
     2521 gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa
     2581 tgtatcaatc acttaataag ttaagtgcgt tacctgacga tacattggta tgttgtgctc
     2641 atgaatatac gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca
     2701 taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg
     2761 taattctgaa aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa
     2821 ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa
     2881 ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg
     2941 atcggtcgtc ttttaagcaa ctattgacac acacatgaag gcaaaagcga tattactcgc
     3001 ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct
     3061 ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga
     3121 tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat
     3181 gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag
     3241 ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag cagggcaagt
     3301 taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga
     3361 tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg
     3421 gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc
     3481 aacaactgcc gcgctgaaca tgatgcagcg tctgaacaag atgtttgacg gcgactggct
     3541 tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa
     3601 caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca
     3661 gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt
     3721 acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gcagcccggt
     3781 tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa
     3841 cgctggcgta aaaggctcca cgctgggcgc aagtggcccg cagtacgtga tggtgccaaa
     3901 gaagcatgca gatcaactgc gtgattctct cgcttcaggc gaaattgctg ctgtacagtc
     3961 gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga
     4021 cacgctttca agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa
     4081 caaactgcgc ggatctaagc tgaagccagg ccaaagtttg acgattggtg caggcagtag
     4141 cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc
     4201 gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag
     4261 tgacactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacaacat
     4321 gccagattcc tgacaaacca gataataaaa aaggcaccga ttcccccggt gcctttttta
     4381 tttatgccgc tttatgcgct tctaccatga tgatatcact ggtgaaagag ccatcagtct
     4441 gcaaggaaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt
     4501 ctactaacgc ttctggcgta cgcattctca cgacccatga agaaaattcc agcggtaact
     4561 tatctgtaat taaattatca actataagat tggcttcatt gattaacgtc aaccacgcac
     4621 cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc tgtaaccaga
     4681 tgtcgcgcac tgggtgaccc ggagacatta catccatcac aatcagccta ccgccaggtt
     4741 tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga tgggcagaat
     4801 aacggctgat aacaatatca aatgcgttat cggcaaatgg cagacttgcg gcatatccct
     4861 ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccacg acatccagca
     4921 tgtgggccga taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg ccaaacgttg
     4981 gtctgccctg tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa
     5041 cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttca ctgacgttca
     5101 gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg
     5161 taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc
     5221 tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg tgagctatct
     5281 gatcaccaat aggaagtaac tgcttactat agacatccac gcccagactg aagttgacgg
     5341 cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg
     5401 ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat
     5461 gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct
     5521 gatcggcggc aagatagtta gcggtcgcaa actgtactaa ctctggcgtt gtctgcgctt
     5581 cctgcaataa caccaggtgt gcatctttgc cgtagttctt taataccgac aaccattcag
     5641 cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg
     5701 gttccccaag tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt
     5761 ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg
     5821 aaacctctat tacgtcaacc cttccgcact cgggagggtt ctactattat agggatttta
     5881 gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta caccggtcag
     5941 aattttgctc attatggaga acattaaatc gcatgacata tcacgcatcc tgatggttta
     6001 atgacaaata aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac
     6061 gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc
     6121 ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa
     6181 acttggcgtt agcctgctta atcggaccac gcgacaactt agcctgacgg aagaaggcga
     6241 gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag aatcagaaat
     6301 tatggagacg cgtaatacac cgcgtggact gttacggatc gatgccgcaa ctccagtggt
     6361 gctgcacttt ctgatgccgt taattaagcc tttccgtgaa cgctatccgg aagtcacttt
     6421 gtcgctagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat
     6481 acgcgctggt acgttaacgg attccagctt acgtgccagg ccgttattta acagttatcg
     6541 aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa cgatcgacga
     6601 tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata cctggccgat
     6661 agcctgtagt gatggacaat tacatgaggt gaaatacggt ttgtcatcca atagtgggga
     6721 aacactgaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg actacatgat
     6781 cgacagagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag tgttgccagt
     6841 ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc gcatccgggc
     6901 ttttatcgat ttccttagcg agcatgtaaa aacagctccc ggaggagctg tcagagaggc
     6961 ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc aatccagtgc
     7021 ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa gattactttc
     7081 caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca tagcccacgc
     7141 cagaatcact tgtgccggag tcgcattgtg tttagctgcg atacgagcaa taacctcatc
     7201 tttcagggcc ttaccatacg ccagcgtcat ataggaagta atatggatgc cgtgctgttt
     7261 agcccaggca accactttac ggttttgcag ataaggagag agttcaatct ggttagtagc
     7321 gatgttttca gcgccaacag cagcaatcgc cttttccatc aatgggatcg tgaagttgga
     7381 aataccgatc tcacgcgtca gcccttgttt tttggcttcc agcagcgcct gcataacccc
     7441 ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat caacataatc
     7501 ggtacgcaat ttttgcaggc tctctttcag acttgggatc aatttgtctt tgctgagatt
     7561 ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac tttctgcaat
     7621 cgcctgacct actgcggctt cgttatcata gatttgtgcg gtatcaattg cgcgataacc
     7681 aagttcaagc gccgttttca cagatgaaat aacaacgtag tctttcaacc taaaccaaat
     7741 gcagggatag ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg
     7801 gcttcagact gttgaggaaa aaagcgtaaa atgcagagga tttttgcgat tctggcaata
     7861 atagatatac agacgaaaaa aaacctcgag cgttaactcg aggcttctta ataagtggcg
     7921 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
     7981 aactaccgct ccaccgaatt cttttacaac caccggtttt atgaccggct tactgcttaa
     8041 tttgatgcct ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta
     8101 cggc
//
LOCUS       DAJVTY010000170         8060 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVTY010000170 DAJVTY010000000
VERSION     DAJVTY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.170"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..572
                     /gene="ttdA"
                     /locus_tag="OQQ66_003503"
     CDS             <1..572
                     /gene="ttdA"
                     /locus_tag="OQQ66_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS1906289.1"
                     /translation="VEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTL
                     PGRSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILR
                     PIGSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVS
                     TGCWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            569..1174
                     /gene="ttdB"
                     /locus_tag="OQQ66_003504"
     CDS             569..1174
                     /gene="ttdB"
                     /locus_tag="OQQ66_003504"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1906290.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1223..2686
                     /gene="ttdT"
                     /locus_tag="OQQ66_003505"
     CDS             1223..2686
                     /gene="ttdT"
                     /locus_tag="OQQ66_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1906291.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLDYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2729..3742)
                     /gene="tsaD"
                     /locus_tag="OQQ66_003506"
     CDS             complement(2729..3742)
                     /gene="tsaD"
                     /locus_tag="OQQ66_003506"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1906292.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            3979..4194
                     /gene="rpsU"
                     /locus_tag="OQQ66_003507"
     CDS             3979..4194
                     /gene="rpsU"
                     /locus_tag="OQQ66_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1906293.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4305..6050
                     /gene="dnaG"
                     /locus_tag="OQQ66_003508"
     CDS             4305..6050
                     /gene="dnaG"
                     /locus_tag="OQQ66_003508"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1906294.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMDQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6245..>8060
                     /gene="rpoD"
                     /locus_tag="OQQ66_003509"
     CDS             6245..>8060
                     /gene="rpoD"
                     /locus_tag="OQQ66_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1906295.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSE"
BASE COUNT         1919 a         2170 c         2182 g         1789 t
ORIGIN      
        1 cggtagaaat ttttgacgaa gtaaacaccg gcaaaaatac cggtagcggc gtaccgtggg
       61 tcacctggga catcatcccc gacaatgacg atgcggaaat cgaagtttac atggcaggtg
      121 gcggctgcac gctacctggc cgctcgaaag tgttaatgcc gtcagaaggc tacgaaggcg
      181 tggtgaaatt cgtcttcgaa aatatctcca ccctcgccgt aaacgcctgt ccaccggtac
      241 tggtgggcgt gggcatcgcc acctcggtgg aaaccgccgc cgtactctcg cgtaaagcca
      301 ttttgcgccc gattggcagc cgccatccca atccaaaagc ggcagaactg gagctacgcc
      361 tggaagaagg actcaaccgt ctggggattg gtccacaagg gctgactggc aacagttcag
      421 tgatgggcgt acatatcgaa tctgccgccc gccatccgtc aaccatcggc gttgctgtct
      481 ctaccggctg ctgggcgcat cgtcgcggca cactgctggt tcatgccgat ctcacctttg
      541 aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc ctgacaaccc cgatcaaagc
      601 tgaagatctg caagatattc gcgtcggcga tgtgatctac ctgaccggta cgctggtgac
      661 ctgccgtgac gtttgtcacc gccgcttgat cgaactgaaa cgtccgatcc cttacgatct
      721 caacggcaaa gcgattttcc acgctggccc catcgtgcgc aaaaacggcg ataaatggga
      781 gatggtttcc gttggcccga caaccagtat gcgtatggaa agttttgaac gtgaatttat
      841 cgagcagacc ggcgtgaaac tggtggttgg caaaggcgga atggggccgc tgaccgaaga
      901 aggctgccag aaattcaagg cgctacatgt gattttcccg gcaggctgcg cggtgctggc
      961 ggcaacccag gtggaagaga ttgaagaagt gcactggaca gagctcggaa tgccggagtc
     1021 gctgtgggtc tgccggggca aagagttcgg cccgctgatt gtctctattg atacccacgg
     1081 caacaatctg atagccgaaa acaaaaagct gttcgccgaa cgccgcgatc ccatcgtgga
     1141 agagatctgc gagcacgtcc attacatcaa ataaccctcc cggagaggct cgccccctct
     1201 cctttttcgc aggcataaca cgatgaaacc ttccactgaa tggtggcgat accttgcgcc
     1261 gctggcggtc atcgccatta ttgctctaat tccggttccc gcagggctgg agagtcatac
     1321 ctggctctac tttgccgttt ttactggcgt gatcgttgga ctgatcctcg aacccgtgcc
     1381 gggtgccgtg gtggcgatgg tgggtatctc catcatcgcc atactctctc cctggctgct
     1441 gttcagcccc gaacagctcg cccagccagg ctttaagttc accgccaaat ccctctcctg
     1501 ggcagttttt ggtttttcta attcggttat ctggctcatt ttcgccgcct ttatgtttgg
     1561 cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg ctgattctgg tgaaaaagat
     1621 gggacatcgc acgctgtttc tcggctatgc ggtgatgttc tccgagctga tcctcgcacc
     1681 tgtaacaccg tccaactcgg cgcgtggtgc ggggattatc tatcccatca tccgtaacct
     1741 gccaccgctc tatcaatcac aaccaaacga cagcagttcg cgcagcattg gctcgtacat
     1801 catgtggatg gggattgttg ccgactgcgt gaccagcgcc attttcttga cggcgatggc
     1861 accaaacttg ctgttaattg gactgatgaa aagcgcatct cacgccacgc tgagttgggg
     1921 cgactggttc ctcgggatgt tgccgctcag tattttgctg gtgctgctgg ttccctggct
     1981 ggattatgtg ctgtacccgc cggtactgaa gtctggcgat caggtgccgc gctgggcaga
     2041 gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa aaacggatgc tggggctgat
     2101 ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat atcgatgccg cgatggtcgg
     2161 ttacagcgtg gtggcactga tgctgcttct gcgcattatc agttgggacg acattgtcag
     2221 taataaagcg gcgtggaacg ttttcttctg gctggcctcg cttatcaccc tcgctaccgg
     2281 actcaacaac actggtttta ttagctggtt tggcaaactg ttagcaggca gcttaagcgg
     2341 ttattcgcca acgatggtga tggtggcgtt gattgtggtg ttttatctgc tgcgctactt
     2401 tttcgccagc gccacggcgt atacctccgc cctcgcaccg atgatgattg ccgctgcgct
     2461 ggcgatgccg gaaatcccgc tgccggtgtt ctgcctaatg gttggcgcgg caattggtct
     2521 ggggagcatt cttacaccat acgccaccgg acccagtccg atttactacg gtagtggtta
     2581 tctgccaacg gcggattact ggcgactggg ggcgattttt gggctgatat tcctcgtgtt
     2641 gctggtgatt accggcttac tgtggatgcc cgtggtgttg ctttaagtga ggacggtgcc
     2701 ggaaattgct ctccggcacc caatagtttt acgcagccgg taactccgcc agcggccagc
     2761 gcggacgcac gctaacgccg agatccgccg tcgcgcctgc tttaaaccgc accattccgg
     2821 cataggcgat catcgcgccg ttatcagtac aaaactccgg acgcgcgtag aacacttcgc
     2881 cgcggcgttt tttcatcatt tccgccagct tcgcccgtaa cgtgcggtta gcactcacgc
     2941 cgcccgccat gaccagtcgc ttaaagcccg tctgatccaa cgctcgctta cacttaatca
     3001 tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat atcagcacgc gtctggtcgt
     3061 cggtgccgtt gtcacgaatc gtgttcgccg caaaggtttt cagaccagaa aagctgaaat
     3121 ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa gcgcccggca gtaccctgag
     3181 ccgccatttt cgacagtaac ggtccgccag gataatccag ccccagcagc ttcgcggttt
     3241 tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc gagcagctcg tactgaccaa
     3301 tgccagtcac gctgattaac tgcgtatgac cgccggacac cagcagcgcg acaaacggaa
     3361 attccggcgg gttatcttcc agcatcggcg ctaacagatg cccttccata tggtgtaccg
     3421 ggattgccgg aacgttccag gcaaacgcca gagaacgccc cacggttgcg ccaaccagca
     3481 gtgcgccgac taatccaggg cctgcggtat aggccacagc atcaatgtct tttgccgtta
     3541 agccagactc ctttagcgcc gcctggatca acggtacggt tttgcgcacg tgatcgcggg
     3601 aggccagttc aggcacgacg ccgccgtagt cagcgtgcaa tttcacctga ctatacaatt
     3661 ggttggctaa caaacctttt tcatcgtcgt aaatggcgat gccggtttca tcgcaggaag
     3721 tttcaatacc cagtacacgc atgacttgtt ttacctcgct ttattaccgc gcagtgtagg
     3781 accaatgcgg gttgatgtaa aactttgttc gcccctggag aaagcctcgt gtatactcct
     3841 cacccttata aaagtccctt tcaaaaaggc cgcggtgctt tacaaagcag cagcaattgc
     3901 agtaaaattc cgcaccattt tgaaataagc tggcgttgat gccagcggca aaccgaatta
     3961 atcaaaggtg agaggcacat gccggtaatt aaagtacgtg aaaacgagcc gttcgacgta
     4021 gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg ttctggcgga agttcgtcgt
     4081 cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg ctaaagcttc tgcagtgaaa
     4141 cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca ctcgtctgta ctaattcccc
     4201 gagagcgttg ctctccgatc agaccgagtt gtagttgtaa ggccgtgctt ccgaaaggaa
     4261 tgcgcggctt attttcgttt atgaattgct aaaaatcggg gcctatggct ggacgaatcc
     4321 cacgcgtatt cattaatgat ctactggcac gcactgacat cgtcgatctg atcgatgccc
     4381 gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg ttgtccattc cacaacgaga
     4441 aaaccccgtc attcaccgtt aacggtgaga aacagtttta ccactgcttt ggatgtggcg
     4501 cgcacggcaa cgcgatcgac ttcctgatga actacgacaa gcttgagttc gtcgaaacgg
     4561 tcgaagagct ggcagcaatg cacaatcttg aagtgccatt tgaagcaggc agcggcccca
     4621 gccagatcga gcgccatcag aggcaaacgc tttatcagtt gatggacggt ctgaatacgt
     4681 tttaccaaca atctttacaa caacctgttg ccacgtctgc gcgccagtat ctggaaaaac
     4741 gcggattaag ccacgaggtt atcgctcgct ttgcgattgg ttttgcgccc cccggctggg
     4801 acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg ccagtcattg attgatgcgg
     4861 ggatgttggt cactaacgat cagggacgca gttacgatcg tttccgcgag cgggtgatgt
     4921 tccccattcg cgataaacgc ggtcgggtga ttggttttgg cgggcgcgtg ctgggcaacg
     4981 atacccccaa atacctgaac tcgccggaaa cagacatttt ccataaaggc cgccagcttt
     5041 acggtcttta tgaagcgcag caggataacg ctgaacccaa tcgtctgctt gtggtcgaag
     5101 gctatatgga cgtggtggcg ctggcgcaat acggcattaa ttacgccgtt gcgtcgttag
     5161 gtacgtcaac caccgccgat cacatacaac tgttgttccg cgcgaccaac aatgtcattt
     5221 gctgttatga cggcgaccgt gcaggccgtg atgccgcatg gcgagcgctg gaaacggcgc
     5281 tgccttacat gacagacggc cgtcagctac gctttatgtt tttgccggat ggcgaagacc
     5341 ctgacacgct ggtacgaaaa gaaggtaaag aagcgtttga agcgcggatg gatcaggcga
     5401 tgccactctc cgcatttctg tttaacagcc tgatgccgca agttgatctg agtacccctg
     5461 acgggcgcgc acgtttgagt acgctggcac tgccattgat atcgcaagtg ccgggcgaaa
     5521 cgctgcgaat atatcttcgt caggaattag gcaacaaatt aggcatactt gatgacagcc
     5581 agcttgaacg attaatgcca aaagcggcag agagcggcgt ttctcgccct gttccgcagc
     5641 taaaacgcac gaccatgcgt atacttatag ggttgctggt gcaaaatcca gaattagcga
     5701 cgttggtccc gccgcttgag aatctggatg aaaataagct ccctggactt ggcttattca
     5761 gagaactggt caacacttgt ctctcccagc caggtctgac caccgggcaa cttttagagc
     5821 actatcgtgg tacaaataat gctgccaccc ttgaaaaact gtcgatgtgg gacgatatag
     5881 cagataagaa tattgctgag caaaccttca ccgactcact caaccatatg tttgattcgc
     5941 tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg cacgcatggt ttaagcaacg
     6001 aagaacgcct ggagctctgg acattaaacc aggagctggc gaaaaagtga tttaacggct
     6061 taagtgccga agagcgatcg ggaagccccc gacagccgca ctgagaggca gcggcaaata
     6121 tataagtacg ccctcgtaat tatcgttggc ggtaaacaac cgttggattt cagcgttaac
     6181 ggctgaagga catcgggtca atcgcccaac accaacctca tgaaataagt gtggataccg
     6241 tcttatggag caaaacccgc agtcacagct gaaacttctt gtcacccgtg gtaaggagca
     6301 aggctatctg acctatgccg aggtcaatga ccatctgccg gaagatatcg tcgattcaga
     6361 tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc attcaggtga tggaagaagc
     6421 accggatgcc gatgatctga tgctggctga aaacaccgcg gacgaagatg ctgccgaagc
     6481 cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg cgcacgactg acccggtacg
     6541 catgtacatg cgtgaaatgg gcaccgttga actgttgacc cgcgaaggcg aaattgacat
     6601 cgctaagcgt attgaagacg ggatcaacca ggttcaatgc tccgttgctg aatatccgga
     6661 agcgatcacc tatctgctgg aacagtacga tcgtgttgaa gcagaagaag cgcgtctgtc
     6721 cgatctgatc accggctttg ttgacccgaa cgcagaagaa gatctggcac ctaccgccac
     6781 tcacgtcggt tctgagcttt cccaggaaga tctggacgat gacgaagatg aagacgaaga
     6841 agatggcgat gacgacagcg ccgatgatga caacagcatc gacccggaac tggctcgcga
     6901 aaaatttgcg gaactacgcg ctcagtacgt tgtaacgcgt gacaccatca aagcgaaagg
     6961 tcgcagtcac gctgccgctc aggaagagat cctgaaactg tctgaagtat tcaaacagtt
     7021 ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc atgcgcgtca tgatggaccg
     7081 cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt gagcagtgca aaatgccgaa
     7141 gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc gatacctggt tcaacgcggc
     7201 aattgcgatg aacaagccgt ggtcggaaaa actgcacgat gtctctgaag aagtgcatcg
     7261 cgccctgcag aaactgcagc agattgaaga agaaaccggc ctgaccatcg agcaggttaa
     7321 agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc cgccgtgcga agaaagagat
     7381 ggttgaagcg aacttacgtc tggttatttc tatcgccaag aaatacacca accgtggctt
     7441 gcagttcctt gacctgattc aggaaggcaa catcggtctg atgaaagcgg ttgataaatt
     7501 cgaataccgc cgtggttaca agttctccac ctacgcaacc tggtggatcc gtcaggcgat
     7561 cacccgttct atcgcggatc aggcgcgcac catccgtatt ccggtgcata tgattgagac
     7621 tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa gagatgggcc gtgaaccgac
     7681 gccggaagaa ctggctgaac gtatgctgat gccggaagac aagatccgca aagtgctgaa
     7741 gatcgccaaa gagccaatct ccatggaaac gccgatcggt gatgatgaag attcgcatct
     7801 gggggatttc atcgaggata ccaccctcga gctgccgctg gattctgcga ccaccgaaag
     7861 cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc gcgcgtgaag cgaaagttct
     7921 gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg ctggaagaag tgggtaaaca
     7981 gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg aaggcgctgc gcaaactgcg
     8041 tcacccgagc cgttctgaag
//
LOCUS       DAJVTY010000171         7820 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVTY010000171 DAJVTY010000000
VERSION     DAJVTY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.171"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(439..1173)
                     /locus_tag="OQQ66_003510"
                     /pseudo
     CDS             complement(439..1173)
                     /locus_tag="OQQ66_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1332..1691)
                     /locus_tag="OQQ66_003511"
     CDS             complement(1332..1691)
                     /locus_tag="OQQ66_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906296.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLFRQ"
     gene            1798..2691
                     /locus_tag="OQQ66_003512"
     CDS             1798..2691
                     /locus_tag="OQQ66_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1906297.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2826..4046
                     /gene="mlc"
                     /locus_tag="OQQ66_003513"
                     /gene_synonym="dgsA"
     CDS             2826..4046
                     /gene="mlc"
                     /locus_tag="OQQ66_003513"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1906298.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            4171..4866
                     /gene="bioD"
                     /locus_tag="OQQ66_003514"
     CDS             4171..4866
                     /gene="bioD"
                     /locus_tag="OQQ66_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1906299.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4819..6111)
                     /gene="clcB"
                     /locus_tag="OQQ66_003515"
     CDS             complement(4819..6111)
                     /gene="clcB"
                     /locus_tag="OQQ66_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1906300.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6270..6884)
                     /gene="dmsD"
                     /locus_tag="OQQ66_003516"
     CDS             complement(6270..6884)
                     /gene="dmsD"
                     /locus_tag="OQQ66_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1906301.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6927..7781)
                     /locus_tag="OQQ66_003517"
     CDS             complement(6927..7781)
                     /locus_tag="OQQ66_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1906302.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCASLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
BASE COUNT         2095 a         1979 c         2013 g         1733 t
ORIGIN      
        1 taatactttt ttcattgtca taccctcaat ttgttttttc atttaacccc actgcggggc
       61 cgttgaaata actatatccc tgggaatggc agacttccgt gagtggttgg tttcagcgtg
      121 taacgatatg tacaaacgct gaataaatta cagcattgat aataacgtat tgtgacttaa
      181 gggaaattta gctaccaata atagtagtct tgatcgggtt aactatttta ctacttacaa
      241 agcggtagaa taactgcgca tcagtaataa atgacacaca gcaaaatgaa tccgtttatt
      301 tgggtactta taatcctgat gacgctagac gcactgcggg aattggctgg cgcttcgtct
      361 attttaggat ggctattaac gttggtttga gcaagcaata agtccggacg tgtagttacc
      421 gcagtccggg cttatttttc aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag
      481 agcaatgacc agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca
      541 gaaaacatca cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag
      601 cgaggaggcc tggcctttag cgcgttttgc gcgggggccg atccagctgc tggcaactga
      661 gtgggctgcg aagaatcctg ctgagaagag taacattccg gcaaagatca gccacagcga
      721 gctgaataag gtcatcagta aaccaaacag cataaccccc gtcgaaaaca acatcaccgg
      781 accgcgcccg tagcgggtgg tcatggttcc ggctttgggt gagctccgtg taccggtcaa
      841 ataagccagc gataataagc caaccacggc ctgactgaca tgctaggggg aaagcatcaa
      901 ccgatagccg atgtaattaa acagcgtgac gaacgacccc atcagcaaaa agccttctgc
      961 gaacaataac ggtaatcccc ggtcacgcca gtgcagacga aagttgataa acaacgtctt
     1021 aggtcgcagc gaagtcgggc gaaaatggcg tgattccgga aggattttcc agaacatcaa
     1081 cgccgaggct agcgcgaaac aaccgattgc cgccagagca attcgccagt tgaaaaagtc
     1141 cgtgaagaca ccactaatta agcgtccgct catgccgcca attgagttgc cgctgatata
     1201 caaccccatc gaaaaggcca cgaaactggg gtggatttcc tcgctaagat aagtcatgcc
     1261 aacagctgcc acgccactta acgaaagccc aatcaaggcg cgcataatca aaatgccgtg
     1321 ccagctggtc atcattgtcg aaataacgta caaatggagg ccaacagtag cgccgtgacc
     1381 atcactggtt tgcgaccaat ggcatcggat agggggccag taaacagcaa accaatagcc
     1441 aacatcgccg tggaaatgga cagtgaaata ctactgttcg cgggggttaa gccaaactcc
     1501 tgcgaaagca ccggaaggat aggctgcaca caatagagga gtgcaaaggt tgccagtccg
     1561 gcagagaaca gcgccagggt gacgcgcata aattgcggcg taccgcgttt aataaattga
     1621 tttggctgag aaatgctttg cttgtcagtg tcgcttgccg gagcgccatc aacagttgta
     1681 gtacggctca cttgaaatcc ttgctaaata tgcctgtgga tcaggcttat acataggtta
     1741 ggaaaatcga attgttctgt ctaatatatt aataatctca aataagatgt tttaaatatg
     1801 aatattgaac ttcgtcatct gcgttacttt gttgctgttg cggaagagct gcatttcggg
     1861 cgcgccgctg cccgcctgaa tatttcgcaa ccaccgctaa gtcagcagat tcaggcactg
     1921 gagcaacaaa ttggtgcccg actgctggca cgaactaatc gcagtgtatt gctgacggca
     1981 gcaggaaaac agtttcttgc agatagtcgg caaatcctgt ctatggtaga tgacgctgcc
     2041 gctcgcgctg aaagactgca tcagggtgaa gcgggggagt tgcgcattgg ttttacttcg
     2101 tccgctcctt ttattcgggc ggtgtccgat acgttatcgc tgtttcgccg tgattatccc
     2161 ggtgtccatt tacaaacccg cgaaatgaac actcgtgagc aaatcgctcc gctcattgaa
     2221 ggaacgctgg atatgggatt gctgcgtaac acagcgttac cggagacgct tgaacacgca
     2281 gtcatcgtcc atgaaccgct tatggcgatg atcccgcacg atcatcccct ggcaaataac
     2341 ccgaatgtaa cgttggctga actggcgaaa gaacccttcg tcttttttga tccgcacgtc
     2401 gggacagggc tgtatgacga tattctcggg ctgatgcgac gttaccattt gacgcccgtc
     2461 atcactcagg aggtgggcga ggcaatgacc atcatcggtc tggtttccgc cggtctgggt
     2521 gtttcaattt tgcctgcttc atttaaacgt gctcagctca acgaaatgcg ctgggtgccg
     2581 attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca tcatgaacaa
     2641 agtccagctt cgcgtaactt tcgtattcat ctgctgaatg ctctcaggtg agggaaattt
     2701 cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgtc taagtaaaaa tttgacgaca
     2761 cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa tatagggagt
     2821 atgcggtggt tgctgaaaac cagcctgggc acattgatca aataaagcag accaacgcgg
     2881 gcgcggttta tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat ctttcccgtc
     2941 tggcgcagct ggctcctgcc agtatcacta aaattgtccg tgagatgctc gaagcacact
     3001 tggtgcaaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg gtgggactgg
     3061 tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggt gagattttcc
     3121 ttgctctgcg cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa ctggcgttaa
     3181 aagatgactt gccattgctg gatcgtatca tttcccatat cgatcagttt tttatccgcc
     3241 accagaaaaa acttgagcgt ctaacttcga ttgccataac cctgccggga attattgata
     3301 cggaaaatgg tattgtacat cgcatgccgt tctacgagga tgtaaaagag atgccgctcg
     3361 gcgaggcgct ggagcagcat accggcgtac cggtttatat tcagcatgat atcagcgcat
     3421 ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg attcaggtgg
     3481 ttatcgatca caacgtgggg gcgggcgtca ttaccgatgg tcatctgcta cacgcaggca
     3541 gcagtagtct cgtggaaata ggccacacac aggtcgaccc atatggtaaa cgctgttatt
     3601 gcgggaacct cggctgcctc gaaaccatcg ccagtgtgga cagtattctt gagctggcac
     3661 agctgcgtct caatcaatcc atgagctcga tgttacatgg gcaaccgtta accgtggact
     3721 cattgtgtca ggcggcattg cgcggcgatc tactggcaaa agacatcatt accggggtgg
     3781 gcgcgcatgt cgggcgcatt cttgccatca tggtgaattt atttaaccca caaaaaatac
     3841 tgattggctc accgttaagt aaagcggcag atatcctctt ccctgtcatc tcggacagca
     3901 tacgtcagca ggcccttcct acgtatagtc agcacattag cgttgagagt actcagtttt
     3961 ctaaccaggg cacgatggca ggcgctgcgc tggtaaaaga cgcgatgtat aacggttctt
     4021 tgttgattcg tctgttgcat ggttaacatt ttttaactgt tctaccaaaa tttgcgctat
     4081 ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt tcctagttta
     4141 tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat taccggtaca
     4201 gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt agcctcccag
     4261 ggaaaaacag ttgcgggata taaacccgta gcgaagggga gcaaagagac acccgaaggg
     4321 ctgcgtaata aagatgccct ggtgttgcag agtgtttcaa ccatcgaact gccttatgaa
     4381 gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag ttgcccaatc
     4441 aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga tcatgtcgtg
     4501 gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccact ctctgaatgg
     4561 gtggtgcagg aacagttgcc ggtgttgatg gttgtcggta ttcaggaagg ttgcattaac
     4621 catgcactgc taacagctca ggcgattgcc aacgacgggc tgccgctcat tggctgggtg
     4681 gctaaccgaa tcaacccagg actggcgcat tatgcggaaa tcattgatgt gctgggtaaa
     4741 aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga acagcgagaa
     4801 ctggggcaat acatccgctt agctatgctg cgcagtgtgc tggcggtaga tagagtcacg
     4861 gtgtaacgtc cgcgaaatta ccgacgcaat gacgcaggca atcaataaac cggggagtag
     4921 ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg cgtgcgtggt
     4981 tgccgccaac agtgtcgcca tctcggtcaa tccgagtaaa agcgtaattt cttcgccatc
     5041 agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac cgataaatag
     5101 cgtcggtgta aagacaccac cgggtgcgcc ggaaccgcta ctcgccagca cggcacacag
     5161 tttacaaagg aagatcccgg caatgatcat taacagtggt ggggcggtta aaaaggattg
     5221 tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca gacccacgat
     5281 caacccgcct agtgccagtt gccagggcgg cgcaagtttg agactcacaa atccacgatg
     5341 acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca cacctgtact
     5401 gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgt tgtagagtaa
     5461 cgcgtcgcta tgattaatca gattgctaac cagcaatgcc acgacggcgg aaataatcac
     5521 cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa ataaactgcc
     5581 agcaagcggg gcacgatagg ccgcagccat tcccgccgcg gcgccacagg cgatccataa
     5641 tttccactcc tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa gggcagctaa
     5701 aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca gcagcgaggc
     5761 aagcgattta accaggcttg ctgcgtaatc gaactgtcca tcggtttgca acgcttccat
     5821 gtaatcggtc ggcgcatgag ggcgttgttg ggtaaatttc tgccagccca tcagcaacaa
     5881 acccgccgcc agtccgccga gcgccggagt tagcaaccgt cgccaggggg aaaggtttgt
     5941 cgctgcattg accagactgc cggagtcatt attgaggaac aaccactcca gtagcagcat
     6001 cgcatgacga aacccggcaa cggcaaaggc cgcgagaata ccgacgactg ttgcgataag
     6061 cagacggcgg aacatagtgc gcagatccgg atagatgtgt agatgatgca tgagcagaaa
     6121 tgggcaatga aacacgggcg actattttgc ggcaaaagag gccagccgca aaagcagtgc
     6181 gtgctttaag agataaaaaa acgtgacact gtaacctatt attgccggat tgcgcgtaat
     6241 cgtcaccatc cggcaatatt acggtgatcc tatcgaaaca gcggtttaac cgcgacagga
     6301 attaacagtt gtgattgcca ttgcgccagc gttaagcgag acagttcacc cagtgcacgg
     6361 tagaaagggt gttctgcttt ttcgataaaa acatcaagaa aacgtgttga ccacggaaaa
     6421 aggtgccatg ccagcaattc ttcgcattct gtctggcgac cattctccgc caaccacgca
     6481 gccatcaaca gcagtgaacc aaaatgatct tccggttcgt attgcttcat ttcaaactga
     6541 atgcctttct cgcgcatcca ctgacgaagt gccaatgttg aatcgccaaa cagcacagat
     6601 tcgcgatcca gccagaccga cccccacggc ggagacggca gtgcccacgg gccgacaaac
     6661 aaacgctgcc aggcctgggc gtgagtctct tcacactgtg tctgaaaagc cgtcaccagc
     6721 ggcgctaatg acgcttctgg tagaggccac tgagtttccc agccgtcact ggtgagtacc
     6781 gcaaccagag gtgcggcttc cgcactctct ggagcgtaat aaaacagcgc gcccaacacg
     6841 cgcgccgcga cagaaaaatt atcttgctgt gaaaaatggg tcattcctaa cattctgaaa
     6901 tgtgcgggtt gccccgcacc aatttgttaa cctgcaatag ccattcctac tgtcatgtgc
     6961 aggccataaa agagcacacg cccgatcatc tcaccgccaa gtatgaggat tagccccaga
     7021 ataagtccgg caacgtgagg ttcacggcga cgaatgagcg ggcaaagcca gcatccaaga
     7081 cttgcgcata acaataccac acgccagacc tgtaaggagg cgtaatctgg taccagtgcg
     7141 ctggcttgtt gcacggatga gtgaatagac gccagagaca aaccttgcag cacaatcacc
     7201 ccggcacaag cgatcagcgc cagaacgcta atgatggcaa atggtgtggt attaaatgta
     7261 acgcgagctg cccgcagaat tgcggcagca agtattggac cgctcaacaa taccgtcagg
     7321 aagaaagcca gcgtagtgta accgttatgc caggttggca cggtgtcgat ttgatacacg
     7381 caggtcatca tccagacgaa aatgacgccg agcgccatgc tgacaagtaa ccagagtttc
     7441 cccaacgctt gcggcatttt accgatgacc gccaccagcc accatagtcc accaacggca
     7501 aaaaatatgg aaccggcagc aatctcgtta ctcagaccag aagctccgat tcgattcagc
     7561 gagttaaacg ctcgcagtgg cgatcccagg tgcatgacag aggcaataaa cccgatgccc
     7621 atcaacagcc agaggaaaaa catgccacgg acaatacgct gacggtctgc gtcatttttg
     7681 gcggcgaacc agccaattcc gctaacaatc agcgcgccta ctacgcattg acccaatacg
     7741 gtaaagataa ccagtggcca ttcatgccat ccatttccca ttttacactt cctccggatt
     7801 agccagataa ccagtggtat
//
LOCUS       DAJVTY010000172         7808 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVTY010000172 DAJVTY010000000
VERSION     DAJVTY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.172"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            258..401
                     /locus_tag="OQQ66_003518"
     CDS             258..401
                     /locus_tag="OQQ66_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1906303.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            414..623
                     /gene="cspE"
                     /locus_tag="OQQ66_003519"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             414..623
                     /gene="cspE"
                     /locus_tag="OQQ66_003519"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1906304.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            789..1598
                     /gene="rlmA"
                     /locus_tag="OQQ66_003520"
     CDS             789..1598
                     /gene="rlmA"
                     /locus_tag="OQQ66_003520"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1906305.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="OQQ66_003521"
     CDS             complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="OQQ66_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1906306.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2589..3047)
                     /locus_tag="OQQ66_003522"
     CDS             complement(2589..3047)
                     /locus_tag="OQQ66_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1906307.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3102..3953)
                     /gene="manZ"
                     /locus_tag="OQQ66_003523"
     CDS             complement(3102..3953)
                     /gene="manZ"
                     /locus_tag="OQQ66_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1906308.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3966..4766)
                     /gene="manY"
                     /locus_tag="OQQ66_003524"
     CDS             complement(3966..4766)
                     /gene="manY"
                     /locus_tag="OQQ66_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1906309.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4829..5800)
                     /gene="manX"
                     /locus_tag="OQQ66_003525"
     CDS             complement(4829..5800)
                     /gene="manX"
                     /locus_tag="OQQ66_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1906310.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6263..>7808
                     /gene="yoaE"
                     /locus_tag="OQQ66_003526"
     CDS             6263..>7808
                     /gene="yoaE"
                     /locus_tag="OQQ66_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1906311.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1983 a         1910 c         1939 g         1976 t
ORIGIN      
        1 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
       61 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
      121 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
      181 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
      241 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
      301 atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
      361 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
      421 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
      481 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
      541 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
      601 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
      661 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
      721 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
      781 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
      841 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
      901 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
      961 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
     1021 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
     1081 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
     1141 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
     1201 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
     1261 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
     1321 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
     1381 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
     1441 atccgatgcg tcttcgcggt gatgaagctg tcgcattatt gcagatgacg ccgtttgcct
     1501 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
     1561 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
     1621 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa
     1681 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
     1741 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
     1801 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
     1861 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
     1921 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
     1981 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
     2041 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
     2101 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
     2161 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
     2221 catgaacaac aagccgtgat ggtttttgcg ttcatgcagg catagcgatt gatgtgcata
     2281 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
     2341 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
     2401 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
     2461 gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag
     2521 gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa
     2581 ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt gtcgatattt
     2641 cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc atcttccgac
     2701 aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa aaaacctttt
     2761 tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat cagagccagt
     2821 gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt atagataaga
     2881 atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag caaaggaatt
     2941 gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat cgcgaaggcc
     3001 aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa tcctccagat
     3061 aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc cagcaggccg
     3121 caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag cgggttaact
     3181 tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag gcctggcatt
     3241 aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg gtcagtaatg
     3301 cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc ccccatgaca
     3361 aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc acccatatct
     3421 ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg ggttgccaga
     3481 cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc gctcatcgcg
     3541 ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat cgggtcgcct
     3601 acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc gtcgtcgatc
     3661 tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat cggcgcagcc
     3721 acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt acgagcttcg
     3781 ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa acccagtgcc
     3841 tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa gacgccacga
     3901 atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac catttcgctc
     3961 acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc aggcgcaccg
     4021 gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac agtaccgatc
     4081 acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag
     4141 aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc aaccaccacg
     4201 atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc attcagcatg
     4261 ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat agccacacgc
     4321 attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta
     4381 tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc
     4441 agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc aatgctctga
     4501 tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc aggcgcaact
     4561 gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc gataataata
     4621 ccggttttca tatccccaag aacgataccc accagggtac acgcgattag cggacggtga
     4681 aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat aaataccagc
     4741 acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga cttaagtgtg
     4801 aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc agatccatca
     4861 ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg cgcgcattca
     4921 gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc acctgggttt
     4981 taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg ccgccttcaa
     5041 cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg ccagcatatt
     5101 tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct gttacgcccg
     5161 gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc cgcagccact tcatcactaa
     5221 caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa
     5281 tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc cccattggtt
     5341 ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg gctttttcaa
     5401 ccggtttggc tttcagtgct ttcacgcctt cacggcctgt ttctactgcc agcgcaacca
     5461 gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc attggaatgt
     5521 taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg gcagcattga
     5581 acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg tcgagttttg
     5641 ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga acgaaatcga
     5701 tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc aactgctctg
     5761 cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc ctttattatc
     5821 gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg aatcgattca
     5881 gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata tcgaaaaaat
     5941 tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt cgaaaggtaa
     6001 aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct
     6061 ctgatatgtt taactcccgt ttaacaacca tggagtatag ggcagtagcc cgcagtatgg
     6121 atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct ggcactctat
     6181 gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg tcgaatagct
     6241 attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt gggcggggct
     6301 actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct tcatcgccat
     6361 tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg ggttatcact
     6421 ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca cgctgaccaa
     6481 accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta tgttgttcgg
     6541 ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg aaaaccgcga
     6601 tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca cacagattgt
     6661 catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga tggttaacca
     6721 tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc tggcatccaa
     6781 accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc tgagcttcct
     6841 gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc cgaaaggtta
     6901 cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga ttgcgcgtcg
     6961 caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg atgccatcct
     7021 gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgccgata acccgatgcc
     7081 gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg tactgacgct
     7141 ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgcggt gaaataagct gggttgacgc
     7201 taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca gtctgttccc
     7261 ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag aactgctggt
     7321 ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg cgattatcgt
     7381 cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc gcgggagctt
     7441 tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc tggatgtgct
     7501 ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca ttactgatgg
     7561 tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt
     7621 tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa
     7681 tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata tcaccatcat
     7741 tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac cggcgcacga
     7801 tgaagatg
//
LOCUS       DAJVTY010000173         7717 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVTY010000173 DAJVTY010000000
VERSION     DAJVTY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.173"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            14..985
                     /gene="ruvB"
                     /locus_tag="OQQ66_003527"
     CDS             14..985
                     /gene="ruvB"
                     /locus_tag="OQQ66_003527"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1906312.1"
                     /translation="MPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDH
                     LLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEI
                     HRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRD
                     RFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVR
                     DFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAA
                     IGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPEMP"
     gene            complement(1035..1820)
                     /gene="znuB"
                     /locus_tag="OQQ66_003528"
     CDS             complement(1035..1820)
                     /gene="znuB"
                     /locus_tag="OQQ66_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1906313.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(1817..2572)
                     /gene="znuC"
                     /locus_tag="OQQ66_003529"
     CDS             complement(1817..2572)
                     /gene="znuC"
                     /locus_tag="OQQ66_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1906314.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            2651..3583
                     /gene="znuA"
                     /locus_tag="OQQ66_003530"
     CDS             2651..3583
                     /gene="znuA"
                     /locus_tag="OQQ66_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1906315.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            3599..4921
                     /gene="mepM"
                     /locus_tag="OQQ66_003531"
     CDS             3599..4921
                     /gene="mepM"
                     /locus_tag="OQQ66_003531"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1906316.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5041..6012
                     /gene="lpxM"
                     /locus_tag="OQQ66_003532"
                     /gene_synonym="msbB"
     CDS             5041..6012
                     /gene="lpxM"
                     /locus_tag="OQQ66_003532"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1906317.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(6101..>7717)
                     /locus_tag="OQQ66_003533"
     CDS             complement(6101..>7717)
                     /locus_tag="OQQ66_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1906318.1"
                     /translation="FSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTSESATKAHN
                     RNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCISRVYLETEK
                     EYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARV
                     NSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGKEYITENMH
                     SEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLR
                     FAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDS
                     FAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEIVKTD
                     NYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVKPHPGELP
                     PSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHYVQYVDLQ
                     ENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2092 a         1785 c         1897 g         1943 t
ORIGIN      
        1 tgccggtacc actttgccgg aagatgtagc agatcgcgcc attcgcccta aattactgga
       61 agagtatgtt ggtcagccgc aggttcgttc acagatggag attttcatca aagcagcgaa
      121 actgcgcggc gatgccctcg atcatttgtt gatttttggt cctccggggt tgggtaaaac
      181 tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat ttacgcacga cttctggtcc
      241 ggtgctggaa aaggcgggcg atctggcagc gatgctcact aaccttgaac cgcatgacgt
      301 gctgtttatt gatgagatcc accgtttatc gccagttgtt gaagaagtgc tgtacccggc
      361 aatggaagac taccaactgg atatcatgat tggtgaaggt ccggcggcac gctccattaa
      421 aattgatttg ccgccgttta ccctgattgg cgcaaccacg cgcgcaggtt cgctgacatc
      481 accgttgcgc gaccgttttg gtattgtgca acgtctggag ttttatcagg tgccggatct
      541 gcaatatatc gttagtcgca gtgcacgctt tatggggctt gagatgagtg atgacggcgc
      601 gctggaagtt gctcgtcgcg ctcgcggtac gccacgtatt gccaaccgtc tgctgcgtcg
      661 agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc tcggcagata tcgctgctca
      721 ggcgctggac atgttgaatg tcgatgctga aggtttcgat tatatggacc gcaaattgtt
      781 gctggcggta atcgataagt tctttggtgg gccggtaggt ctggataacc tggcggcggc
      841 tattggcgaa gaacgtgaaa ccattgagga tgtgctggaa ccttatttga ttcagcaagg
      901 ctttttgcag cgtacaccgc gtgggcgtat ggcgacgacg cgggcgtgga atcactttgg
      961 cataacgccg ccagaaatgc cgtaagtcgg attggtcgct cgcgccgcat ccgacaaatg
     1021 tgttcagcga tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgct
     1081 gcacatagca ccaccgacgg acccgccggc gtatcgtaaa atgcggaaaa ggttaaaccg
     1141 cccgttacgg caaccatccc caccaacaca gcgacacctg ccatctgttc cggcgtgcgg
     1201 gcaaagcgac gtgcagtagc agcaggaata atcagcagtg aagtaataat caacgcgcca
     1261 acaaatttca tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcacg
     1321 cgctgtaatt tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa
     1381 ttgcgccatt gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagcgatg
     1441 agatcttctg gcgtcactgc cagcaaatca ccgaacaggt aagccatcaa atcaacacga
     1501 atgttagaca tcagactaac gaccaccagg cccagcgaca gggcactgtg cgccataatc
     1561 cctaataacg tgtcaagcgc cagctgtgga cgtttctcca gccataccag accgcccgcc
     1621 aggagcagcg taacggcaat taccgcatag aatggattca cgtccagcaa caaaccaaac
     1681 gcgacgccaa gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag
     1741 actacaaacg aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg
     1801 ggaaataata attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta
     1861 aatcgtgacg atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac
     1921 caaacataga aataaactcc ggatgtaggg aaacaacttc cggtgtgccg gaacaacaaa
     1981 tgtggtgatt cagacacagc acttcatcgg tttttgccat taccagatgc agatcgtgag
     2041 agaccattaa aacgccacaa tccagttcgc gacgcaactg gtcaataagg tcatataacg
     2101 ccacctggcc atttacatcc acgccttgag tgggttcatc cagcactaat aattgcggtc
     2161 gatttaacaa tgctcgcgct aacagtacac gctgtgtttc gccaccagag agcttttgca
     2221 tcggtgcgtt aatcagatgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt
     2281 tatgtgtgcc agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga
     2341 gatacagctt ctgcggtaca tagccgatgc gtagttttcc gttgcgcttg ataacccctt
     2401 catcgggtgt taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg
     2461 gcccaagtaa agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc
     2521 ggcgttggcc aaaagaaact gagacatttt ccagggaaac cagacttgtc atgttaattt
     2581 tagtcttgca gtagtcatga gatgttataa tatcacactt ctcatattca ttacgatgat
     2641 tggtcgcatt atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg
     2701 gggaggtgca acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt
     2761 catcgcttct gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc
     2821 ttcagaacat gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt
     2881 agtcgtttgg gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattact
     2941 aggagcgaag caggtaacga ttgcgcagct tgaagatgtg aaactgctac tgatgaaaag
     3001 tattcacggc gatgatgatg atcacgacca cgcggaaaaa agtgaagaag atcaccatca
     3061 cggcgatttc aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc
     3121 aatccatgga aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct
     3181 gaaggatttt gaggcacaat tagcctcaac cgaaacgcag gttggtaacg agctcgcgcc
     3241 gctcaagggg aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt
     3301 cggactgaca ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg
     3361 tttacatgaa ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc
     3421 acagttcagg ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac
     3481 gttggatccc ttggggacga atatcaaact gggtaaaaca agctattcag aattcctgag
     3541 tcaattagcc aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt
     3601 gcaacagata gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt
     3661 tatgttgggg tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta
     3721 tcaccgcgat gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc
     3781 gctcttacct gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc
     3841 ccaggacgaa ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc
     3901 cactggcgat acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac
     3961 ccaactggct gcggccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg
     4021 gacattaacc gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac
     4081 ccgaacctat gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg
     4141 agagtgggtt aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag
     4201 aaacgccggt ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat
     4261 ggatttccgc aaactgaaaa aaggcgatga atttgcggtg ttaatgtctc gagagatgct
     4321 tgatggtaaa cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga
     4381 ttattacgca atccgcgctg aggatggcaa attctacgac cgtaacggta ctggtctggc
     4441 gaaaggattc ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc
     4501 gcgtcgtact aatccggtga ccggtcgcgt tgcaccacac agaggtgttg atttcgccat
     4561 gccgcagggt acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag
     4621 tggcgcagca ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat
     4681 gcacttgcgc aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc
     4741 gctttccggt aataccggac gttcaaccgg gccgcatctg cactatgaag tatggataaa
     4801 ccagcaggcc gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc
     4861 cgatcgtcgc gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta
     4921 attaacatcc attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg
     4981 caagttgcgc cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc
     5041 atggaaacga aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac
     5101 ccgcgctact ggggagcatg gctgggcgta gcagcgatgg tgggtatcgc tttaacgccg
     5161 ccaaagttcc gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa
     5221 agctcacgcc gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct
     5281 gaacgcgaag cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg
     5341 gctgagttgg caatacgcgg accggagaaa attcagccgc gcgttgactg gcaagggctg
     5401 cagatcatcg aagagatgag gcgtaataac gagaaagtta tctttctggt gccgcacggt
     5461 tgggccgtcg atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg
     5521 ttccataatc agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt
     5581 ggcggtcgtc tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag
     5641 gggtactggg gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg
     5701 gatttctttg ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtgtgc
     5761 cgtgcgcgcg ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt
     5821 caggtgcgcc caccgatgga tgatctgtta gaggcggatg atcatacgat tgcgcggcgg
     5881 atgaatgaag aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta
     5941 aaattgctga aaactcgcag accgggcgaa atccaaccgt ataagcgtaa agatctttat
     6001 cctatcaaat aaaaaaaagc ctctcaaatt attgagaggc tgtactcgat aaaaaatctc
     6061 aatgagtgtt cccactattt tgagttttta ggttagtacg ctattgagtt aatcgtagcg
     6121 tgataatttc aaaaggtttg aactcaagat tgcaatattg attgcaataa aaactgtcat
     6181 ctaacttatt ttcttgaaga tcgacatatt gaacgtagtg ataattacat acccacgaaa
     6241 gcatggctga aatttctaat ccatgactct catataatct tacgattata ccgtcgccgt
     6301 cttcagctaa ttttattgtt tcgatgataa cgctgttagt atcgatagca aaaaatgata
     6361 gtgaaggggg taattcaccc gggtgcggtt tgacagggac gacctgtaag ggatgattca
     6421 ttatataggc ttgattaata acaccaccca cacggtaatc tccatgatga ggaaataagg
     6481 agaatatgaa tttttgttta ccaatatctg caaaattatt aatcaggaaa ttatcataat
     6541 tgtctgtctt aacaatttcc ccaggtttat gctgcgaacg cagcaaagtt aattcaagcg
     6601 tctggtcttt gacacgatag ccatatttgt tttttgctat taaagctatg ccagtttctc
     6661 gatcagaaag gtcgacccag tgatgtgctg gtatttcatc tttggcaaaa gcgaacgaat
     6721 catcgctatg cgttggtctg tcaattacac cgaactgaat ttgacattgt gcaagagatg
     6781 tttgtactga caccgggaat ttgactttga gagaaacatt aggttcgtgc caatcaataa
     6841 aagtttggac atcaacgcgt ggactgtcct gtgtcagggt aaatgcgaag cgaagataag
     6901 aggaattgta gtgataaatc agcgtcatcg tagttttagg cccgctgatt acagttgtca
     6961 ttttttcagc gagtaaaaca tatgattccc catcacgata atttgaagca aaatcccagg
     7021 ccgcgaaaaa acctgcatct tcgtggtatg cacggatctc actgtgcata ttttcagtaa
     7081 tgtattcttt accatatctt ttatcatata gcgaaattaa atcaccgttt tctgaaaaaa
     7141 gaagtttaat gtaattattt tcaatacttc gagattctgc cttcagacca tttacgatct
     7201 tattctttgg atcaatcaca gcataaccat aagagtttac tcgtgctttg agccagttgt
     7261 tgtttatatt gatccattcg ttacgagcaa aacaggtggt attaaataaa atgtgcggat
     7321 ctttataaga agaagtatca attttactta ataaagtaga ctgcgtatca gaaataattt
     7381 tctcggtttt tgcttctagt ttaatatatt ccttttcagt ttcaagataa actcttgaaa
     7441 tacatgatcc gggaaggatg tcatggaatt gcaaggtcag catctctttc catatttcat
     7501 caaactcact gcgaagaatt gatgtcatat tattagtaat agttgtaaaa aattcagcat
     7561 catgtaattt attttccata tttcgattat gagctttagt tgctgactct gatgtaaagc
     7621 acccttgatg agcctcgaaa taaagttcac ctgaaataat gggcaagctt tcgcgaaaag
     7681 cgtcggcgta agtgaagaac ttatcaacac ggctgaa
//
LOCUS       DAJVTY010000174         7679 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVTY010000174 DAJVTY010000000
VERSION     DAJVTY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.174"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..77)
                     /locus_tag="OQQ66_003534"
     CDS             complement(<1..77)
                     /locus_tag="OQQ66_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906319.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVI"
     gene            complement(88..786)
                     /locus_tag="OQQ66_003535"
     CDS             complement(88..786)
                     /locus_tag="OQQ66_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1906320.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(786..1127)
                     /locus_tag="OQQ66_003536"
     CDS             complement(786..1127)
                     /locus_tag="OQQ66_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906321.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(1120..4380)
                     /locus_tag="OQQ66_003537"
                     /pseudo
     CDS             complement(1120..4380)
                     /locus_tag="OQQ66_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            4425..4754
                     /locus_tag="OQQ66_003538"
     CDS             4425..4754
                     /locus_tag="OQQ66_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1906322.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            4741..5133
                     /gene="tnpB"
                     /locus_tag="OQQ66_003539"
     CDS             4741..5133
                     /gene="tnpB"
                     /locus_tag="OQQ66_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1906323.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTKNTLLTQ"
     gene            5140..6741
                     /locus_tag="OQQ66_003540"
     CDS             5140..6741
                     /locus_tag="OQQ66_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCS1906324.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(6876..7136)
                     /locus_tag="OQQ66_003541"
     CDS             complement(6876..7136)
                     /locus_tag="OQQ66_003541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906325.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(7184..7393)
                     /locus_tag="OQQ66_003542"
     CDS             complement(7184..7393)
                     /locus_tag="OQQ66_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCS1906326.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(7489..>7679)
                     /locus_tag="OQQ66_003543"
     CDS             complement(7489..>7679)
                     /locus_tag="OQQ66_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906327.1"
                     /translation="KTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGPVHAR
                     LLRQALELIADAESARKK"
BASE COUNT         1668 a         2413 c         1887 g         1711 t
ORIGIN      
        1 cttatcacga agccgcacga ctccgcaggc gcacaccgcc gggcatgcgc cagaatcgct
       61 gattcagtct gtgtcataaa tcgggattta ctgcgaaagt ttattaatgg aaaggaaacc
      121 gccaaaattg ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc
      181 cttacggata tccgtggtgg gtttatcgaa ctcatccgcc acagccccgc ccgtgtaacc
      241 acactcatca gagcggtagg tccacataca ggtgttcgcc agcatgatac gaccgggaaa
      301 cagcgccccg tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag
      361 ctgcgacatc tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg
      421 attgcccgca acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac
      481 cgtggccccc accagactct gcaggtcttc cgccatcccg gtgaccagac cgaacagatt
      541 ggacaccgtc agcgacggtc tggcactgct gccccggccg ttcatctcaa agccgctgcc
      601 gtcaatcggg tatgcctcat acttacgccc ctgccaggtg accggctccc ctttttcatt
      661 cagctcatta cagaaaaaat accgctcacc accctgcacc gtcaggtcaa tttcccagag
      721 taccacccgc ggtgactgct ctgatttaac cgactcgctc agactttctt cgtgaatatc
      781 ctgcatcagt tcaccacctg ctcaatcgta caactgaaat cactgtacct ggcgttatct
      841 gtgacgctcc actcccggca taccaccctc accgtccggt tatgtttcgg cggtcgccac
      901 aaaaaggcac ggtaaccacc atgccacgat aaaaactctt ccagccatcg ccgggtcgcc
      961 tcatccgtca cccggaacac cgcctgaaac gtcttcagtc gggcattaag tcccgtcggg
     1021 cggcgctgtt cataaccgtc accaaaccgt accctcgcca ccgacggttt ctcactcacc
     1081 tgcatccctt cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc
     1141 gtcacgtcgc atggacagca tcaccgcctg cacccgctgg tcaatcagtt gcacaaggct
     1201 gcccgcagct tccggcccta tctggccatt agtcccgtca ttctgaatgg cgatatggta
     1261 gaccggggaa tacaccagac ctgcactgcc gttcatactg cccaccgctc gcacacccag
     1321 cgagccatcc gccgcccggg tcaggggcat aatggcttca ggaccggcct cccccatcag
     1381 tcccgcccct tttgcaaagg caaagtacgt gggcgtatcc acaatactgt tgctgtacgc
     1441 acccaggttt gccgaggtat acacaccgcc ttttgcattg gccaccgcac cgcccagcca
     1501 gctaccaata ctgccgataa atcctcccgc tccggacata ctgtttgccg ccgtcttgat
     1561 cccgttgaca atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc
     1621 ccagctgcgc cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc
     1681 tgagataccc tccgtcgcaa gctgtgctgc ctgtgaggcg taatcggacg cattatccac
     1741 ccagttactg aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc
     1801 ggcataaaag gcttcctgct ctttcagacg ttcactcaga tactgcgcgt tctgtgccag
     1861 agcctgtctg taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc
     1921 atccttctgg cggaaactgt cgcggatctg ctgcaactcc cgcatgcgtt cacggagccg
     1981 ctctccctgc ccgtacccca gcagttccgc ttcatttgat gcacgagcag ccacattatc
     2041 attcttcagg gtctcttccc gggaccgcaa ctgctcccgg attttttgct ggtcaatcag
     2101 ggccgcattg cgcagcagtt cctgcttctg catctccgtc agggttttca gttcgccctg
     2161 cgcagtctgg tatttcagct tcgccagctc tgtattctgc cccgccagtg ccagttgctc
     2221 tttctgctgc ttcagcagcc gggaaaaact gtcttccgct ttttccgtct ctgattttcc
     2281 accccgggat ttgggtttat tcgcctcgtt attgcgccag gcttccaggg cattactgat
     2341 ataacgttgt ctcgcctcct gatacggatc acccacaaaa ccgaggtcat ccgccgcata
     2401 ccccagtcgg acacgctctt tttcttcccc tttcagtctg gacagggcca gctcacgctc
     2461 tgtttttgtc agggcactct gctgtttatc atccagagtg gcctgtggca gccgtaacgg
     2521 cacattcacc agtccctgcc gctgctgaag cagttcatta cccagcccca gcagacggtt
     2581 gaattccgta tgctgaccgt tcataaccag catggactgg tacaccttat tctgctctgc
     2641 cgcctgctga cgaattaacg ccacacgacg gtcttccagc ccggcaagca catcctgaat
     2701 ggactgcgct ttttcctgca tcagtgccag acgggactgc tcaacggcaa gctgctctgt
     2761 tgcctgagca agcccttccg ttacggtctt caccgaggtc agatggttta tcatgaatcc
     2821 gtcaccggtc gtccagcccg ggttcgccag aacatactga tatcctgcga ttttttcctg
     2881 cagggatttc acccgactag cctattcatc aatcagccgg ttctgctctg tcagcgccgc
     2941 ccgtgttcgt ccttcattat ctgaggcttc aggcaaagac attgacggcg ttttatgcgc
     3001 gatttcatct atcgtcagtg catactggcg cgcagactcc caggcctgct cctgattctg
     3061 gtacagcgtg taccatgctg cagcccccag catcaccagt ccgggtacgc caccaaccag
     3121 ccccagcgca ccgctcatca gacgtgagcc caccgccgtt gtactgttca gcgcattctg
     3181 ggcggcgctt ctggcagcaa tatttctgtt caggcgttcc tgtgtggccg ccagacgggc
     3241 ctctgcagca atctgcatct ccgtcccgcg ggctgccgcc acggcctgct gagcacggta
     3301 cacggctgcc cttgcccgcg ccgtggcaat ctgcgttccc ctgaactgtg cttccgccag
     3361 tgcaacttca ttacgtgcag ccgtcacaag tcctgccgtg gcagacatcg ctccggaggc
     3421 catattgcca aagtaccggg caaccccgac ggcaaccagc gcccccacag ctgttgccac
     3481 attatcaatc tgtccggcaa caccgttcag cacgccggag agcgtttttg tcaccccgca
     3541 ggcctcattc acttcgccca cccaggccat aaaggcgttt tccaccttcg tgatactacc
     3601 ggaaaccgtt tccggcatgg ccgcatattc atcacgtaat atccccagct ggctgattaa
     3661 cgcagggacc actttatccg ctgtcagttt tccgtcatcc gccattgcct tcagatcttt
     3721 acgggccaca cccatccccg cagcaagcgc gcggatcacc cggtcgccat tttcgttaac
     3781 agcattaaat tcttcgccac gcagtacacc ctgtgccagt gcctggctga actgggtgat
     3841 caccgaaccg gattccgcaa ctccagcccc tgacagtttc agtcctgtcg aaatggcctc
     3901 cgtcaccttc agcacatcat cagcactgta accatattca cgcatcgagg cagccgaacg
     3961 ggcaaacagg gccgcattat ccgaaaacgc ggtacctgtt cgctgactga tatccatcag
     4021 cactttctgt gatgacgaga attcatccga tgactgagat gcctgtttca gacgggcatt
     4081 cacggaaccc cactcatcgg ccagcgaaat caggtgtccg gtggcaaagg caccggcaaa
     4141 tgcgccagcc attccgacag cagaaccgcg aatttccgtc aactgactgt tcagttctgc
     4201 aagggcgcgg cgctgttccc gtgcggcagc agcggcatga cgtccgccat tctgcagggt
     4261 ccggtaatat tcactgccca tacgggacgc ccgctggatc tccgactgga atgactgtga
     4321 atttgccgaa attttgagtg tcgttacggg gaagtcatct tcacagggat cttcggtcag
     4381 ttagtattac cgacatccat tcaccaactt accggagata atcgatgtca aaaccccgat
     4441 ggaccattga acagaaaagg cagcacgttg ctgcctggcg cgccagtggt cttacacgtc
     4501 agcaatactg tgaactcaac gatattccct tcacatcact tcgcgaatgg ccgcaggatg
     4561 tcgcaaaagc tgaacgccgg gcaaatgaac cgaccgttct tcccgttcat attgcgccac
     4621 cactgcatgc tgatgtacca cagcccgtca cgaatgagcc agtcacgctc ttccttcccg
     4681 gtggagtacg gatgtgctgt cagccgtcac aacttaccga tgtcttcagg gcactcaaat
     4741 atgctcagtc ctgataatgt cttcatcgca attaaacctg ttgacatgcg ccggggcatc
     4801 gactcactga cgcagtatat acaggatgaa ctccggtcga catggcatga gggagccgcc
     4861 ttcgtctttg ttaacaaagt ccgttcgcgt atcaaagttc tccggtggga taaacacggg
     4921 gtgtggttgt gtacccgccg tctgcacaaa ggcagcttcc gctggccacg tgcaaatgac
     4981 gctgcctggc acctcactcc cgacgagttt aactggctga ttgccggtgt tgactggcag
     5041 caggttaagg gacatgacct gacgaaatgg gtctggcaga atgaacctga actgcgccct
     5101 gaaaacacga aaaacacact gctaactcag tgaaaaataa tgaatatccg tatctggagt
     5161 ggtatactcc cctgtatgga tatctccgct ctcaacacca cgaatgacat cgaaaaactg
     5221 cgtgctatgg cacttgccat ggtacaagaa gtcatgtcgg agaatgccga aaaagagcgg
     5281 gaattactgg agaaaagccg gcgcatccag cttctggaag aaatgctgaa actggttcgt
     5341 caacagcgct tcggaaaaaa atgtgaaacg ctggctggta tgcaacgctc cctattcgaa
     5401 gaggatgttg atgccgatat cgccgcgctt accgcacatc tggataaact gctcccgcaa
     5461 tcccctgaag aagatgaaaa agcgtcccgt tcacgcccga tacgcaaacc cttaccggtt
     5521 catcttccac gggtggaaaa aatgatccag ccggacactg accattgccc tgaatgtgac
     5581 gagccgctgc actatatccg cgatgcggtg agtgaaaagc tggagtatat tcccgctcac
     5641 tttgtggtga accgttatgt ccgtccgcaa tacagttgtc cctgttgcca gaaggtgttc
     5701 agcggtgaaa tgccggcaca tatcctcccg aaaagtgccg ttgagccatc agtcatcgca
     5761 caggtgatca tcaataaata cggtgaccac ctgcctctgt atcgccagca acaggtcttt
     5821 gcccgttcag atgtcgggct gcccgtcagt tcgatggctg acatggttgg cgcggcgggt
     5881 gccgcattat ctcccctggc ggcgttactc catcgcgagt tgataaaccg tccggtggtg
     5941 catgcagatg agactaccct gaagatcctg aacacgaaga aaggcggtaa atcctgctcc
     6001 ggttatctgt gggcatacgt cagtggagaa aggacgggac cgtcagttgt gtgcttcgac
     6061 tgccggaccg gacgtagcca tgagtatcct gaaaactggc ttcagggctg gggcgggacg
     6121 ctggttgtcg acggacataa agcttaccgg actctggcaa acaaagtgcc ggagatcacg
     6181 ctggccggat gctgggccca tgcccgcagg ggcttcgccg acctgtataa aatcagtaaa
     6241 gatccacggg ctgccatagc cgtgaagaaa atcgcggggt tgtaccgtct tgagaagaag
     6301 atcagtagcc gccccgtgga aaaaatccgc cagtggcgac agcgttatgc ccgtccgata
     6361 ctggaagaac tgtggtcatg gcttgaagag caggaaccgc aatgttctcc gggaaaggca
     6421 ttacacaaag ctattgccta tgcgctgtct catcgcgtgg aactgagccg cttcctggaa
     6481 gatggtgcgg tgccgctgga taataatgtg tgtgaacggg ccatcaaaaa cgtggttctg
     6541 ggcagaaaat cgtggctgtt cgccggttcg cagatggcgg gagaacgcgc cgcgcaaata
     6601 atgagcttgc tggaaaccgc gaaacgcaac ggtctggagc cgcatgcctg gttgacagac
     6661 gtcctgatgc gtctgccgga gtggccggag gagcgactgg cagagttgct gcctcttgag
     6721 ggatttacct tctccgggtg agtgatacct gccgtcaggt gttcgtgcac cgggccataa
     6781 cctgcagtcg ggagttgaac tcctgacggc aggaaatgag ccagaccagc aaccacgcgg
     6841 gctgacagcg aataagaacc tgaatcagcc cggagtcatt cccgggtcag tacatcatca
     6901 cctgcaatta atccccgagt cagtacttcg cggcctgacg cgatcacgct actcagtact
     6961 tcagagcccg cctccggtac ctgcctggcg atcgcctctg tgcaacaaaa taaatcaacc
     7021 gttatcaaac cacgagcgtt cgtatgcgcc gccgatgacg cgcgcctgaa aaacgcgtca
     7081 atatgagttc cccggaagct tacaattttg ataatcagtt cacgtaacgt cgccattcac
     7141 ctttctccgg gcgtaaaaaa accgcctcag cggttctcat cattcatgac tgtgctgcaa
     7201 agctcagcgc gtcttccagc gccgcaaacg gatccacctc cggcttatcc tcatcctcgc
     7261 cccagcagag catgacgtcc ttcagtgcaa cattcatccc ctgtgccccg aaaaccgctt
     7321 tcacgatctg tgcattacgg atatccccgc gctcatcacc cagcggggat atcctgtcga
     7381 actccatcca catcatcgcc tcgctcgcac tcaggctgtg gcgcagttcg gataaggtgc
     7441 gccccagacg gagcgcaagt cgcatcagaa agcgaatttc cgggcgggct acttttttct
     7501 ggccgactct gcatcagcga tcagttccag tgcctgacgc agcaaccggg catgtaccgg
     7561 accatagacg gccagcacct gctcacggtc gtccggagtg aacacccgct gcaggtccgt
     7621 atcacacagg acatcgcaga acagcgtcac atccgcttcc aagttacggc gggttttcg
//
LOCUS       DAJVTY010000175         7630 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVTY010000175 DAJVTY010000000
VERSION     DAJVTY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.175"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            555..3968
                     /locus_tag="OQQ66_003544"
     CDS             555..3968
                     /locus_tag="OQQ66_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1906328.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4032..5666
                     /locus_tag="OQQ66_003545"
     CDS             4032..5666
                     /locus_tag="OQQ66_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1906329.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5675..6745
                     /locus_tag="OQQ66_003546"
     CDS             5675..6745
                     /locus_tag="OQQ66_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1906330.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6870..7586)
                     /locus_tag="OQQ66_003547"
     CDS             complement(6870..7586)
                     /locus_tag="OQQ66_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906331.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2222 a         1736 c         1790 g         1882 t
ORIGIN      
        1 ccaaatatca tggtattgat acgggagatt atgccgtaca ggtacgacac gttatgtcgt
       61 gccaaagggg agtggataga aaaaatggcg aagatgcgaa aagttgatta atgcattgaa
      121 aaaaaatgaa ttaataatga acttgggtaa aagcgccatc aaaatacgac acgacctgtc
      181 gtattttgtg ttagccggga aaagatatct gctacttact aaaatgattt agtgctgaaa
      241 atatttataa gccaataaat agacaggtat tacggagtaa cgcccaggct attttaacga
      301 gcctgctgtt ttattggctc aaaaaacata tcccatagat gatcagccat acccgcgtta
      361 gtgagcaagg tatcccttga gtagccaggg ctgaagacgc ccattgatgg gcgaaaacca
      421 aggttctggc cgttaaagcg gctatgtcgt taaacacatc accaccatat gccatccggc
      481 tcacttcgtc ggccaaatta gtgtgatatt ctttccagaa tttctgttgt atccacaaca
      541 taaggcgatg attgatggag cagtctctca attttgaaat gttgcgcagc cagtggccgg
      601 aactggcaga actcgcatgt atggcagagc gctatgttca ctccgatccg gaaagctgcc
      661 tggttaagct gcgcaactac accgaattga tggtgcgctg gttgtatcgt caggagcggt
      721 tgccggaagg tattaaggct aatctttacg atttaatgaa cgctgatgtc tttaccagca
      781 tgatgccgga agccatcatc atgaaaatgg atgcgctgcg tatccatggc aaccgtgccg
      841 cgcacggcgg acgtatcaaa gctaaagata cttactggct gctcaaagaa gcgtatttgt
      901 tgggaatttg gctgtatgtt cgctacgccc acggtaatgt tgatgactgc cccaaattta
      961 cactccctcc attaacacaa tcttcaggtc gtgcagatga aaaacgtctg gaagatgcaa
     1021 tcaaggctca ggatgaaagc cgtgagcgcg aactggcgct acaacgcgca ctacagcaag
     1081 aacaggaaaa ggccgaacac ctcactcaac gcctgaatga agccagagcg cgtaaccagc
     1141 atgttgccga tattctctct attgatgaag cggaaacccg ccgtcgcctg attgactctc
     1201 gcttacttgc agccgactgg aatgtagggg aagaacttaa aaacacggat caagtcacac
     1261 aagaacatcc agtcaaagag caacccacca cctctggtga cggttatgca gactatgtct
     1321 tgtgggatga ggcacacaaa ccgttggcgg tggtggaagc aaaaaaaacc agcgtcaatg
     1381 ccgagcaagg acgaattcag gcccggctgt atgcagactg gctggaaaaa gaatacggtc
     1441 agcgtccggt catcttctac accaacggct atgatatctg gctgtgggat gaccataaaa
     1501 ctcatggtta tcccccgcgt cgggtgtttg gcttctacag caaggaaagc ctgcaatatc
     1561 ttattcagca gcgtgaaacc cgtctgccgc tgaacagagt gccacacgta aaagataacg
     1621 aaggtaaggc cgttgccgga cgtttgtacc agctcgaaac cattgcccgc gtcagtgaac
     1681 ggtttaccaa caagtaccga caatcgttaa ttgttcaggc tacgggtaca ggcaaaaccc
     1741 gcgtggcaat tgcactaagc aaactgatga ttgatgcccg ctgggtaaaa cgcgtcctgt
     1801 ttctttgcga ccgtaaagaa ctgcgtaaac aggcggcaaa tgcctttaat caattcacca
     1861 atgagccgct gtatgtggtc gggaaatcga aaaaagcaga caggcagaat gccagaatct
     1921 acatcgctac ctatcccggt atgatgaaaa tcatggacca tttcgatgtc ggttatttcg
     1981 atttgatcat cgccgatgaa tcccaccgct ctatctacaa cgtctacggc gatctgttta
     2041 agtattttga tgccctccag attggcctga cggccacgcc aattgacatg gtgagtaaaa
     2101 ccactttcgg cctgtttggt tgtgaaggac gtattcctac cgccaactac agcctggaag
     2161 atgccatcgc cgataacaat ctggttccct atgaggttgt cacacacaca acagaattcc
     2221 tgcgcgaagg tatcaaacgg gagaaattaa gcgacgcaca aatccgcgag ctggaagaac
     2281 aaggcataga tcccaatacg ctggagtttg atggcaaggc gctggatgag gcgatctaca
     2341 acaaagacac caaccgctat atcctgcgta acctgatgga aaatggcctg aaagatcggg
     2401 acggccagtt gcctggtaaa accatcattt tcgcccgtaa ccacaaacac gcgctgctgt
     2461 tgaatgaatt gttcgacgat atgtacccac agtttgccgg gcgcttctgc caggtcatcg
     2521 acaactacga tccccgcgcc gagcaactga ttgatgattt taaagggctg gatgaaagca
     2581 ccaacaaaga gctgaccatc gctatctccg ttgacatgct cgataccggt attgatgtcc
     2641 cggaaattgt gaatctggtc tttgccaaac cggtcaaatc aaaagtgaag ttctggcaga
     2701 tgattggccg tggtacgcgc ctctgtccgg ggttgtacgg ttacgacgat aacggcaaac
     2761 cgctggataa acagaaattc cgcattttcg atcactgggg caattttgag tatcacgagc
     2821 tgcataccga agaggcggaa gtcacagcga caaaatcgct tgcgcaaaaa cgcttcgaag
     2881 cgtgggttat gctgggggcc gctgcgcagc gtaaattcga caaacaggcg gtggatttag
     2941 ttgctcacca gcttcgcgag caaatcaacg cactggatga aaagtcgatt gccgtacagg
     3001 aaaaatggca gcagaaagcg cagtacagtg atgaaaaggt gctgcgccag ctttctccga
     3061 aaacacagca ggatctgcta tctgttctgg ccccgttgat gcagtggctg gacgtgcgcg
     3121 ggcaaagcga tgccatacgc tttgatatgg atattctggc ggcgcaaact gcccgttata
     3181 ccaacccgga agagctggat gttctctggc cgatcatcgt cgaaaaagtg gagcgcctgc
     3241 cgccgcattt ggcgcaggtg cagcaacagg gacaacgaat caatcagctt cgtaatttaa
     3301 gctggtggaa gcaggccagc ctggaagagc tggaggatat tcgcattcat ttgcgcggta
     3361 tcatgcacct gatggaaaaa gacgcgacgc ctaaatttgg ttcgatacag gtggatatta
     3421 ccgaagatgc gaacctgatc cagacggaga cccgcaaaac taatatccgc tcgattgatt
     3481 tcaaactcta tcgccagcag gttcaggggg cgctggagcc gctgttccag caaaacccgg
     3541 ttctgaagaa aatccgcaac ggcgagccag tcacgcaaaa cgagctggat gagctggcga
     3601 agctggtgct gatccagaac cccaacgttg atattcgtgc gttgaaagag ttttatccgc
     3661 aggcaaccgc cagcctggat aaacttttgc gtaccatcat cgggatggac agcgacgcgg
     3721 tggaagtgcg ttttgcccag ttcgccgctg ataacagcct gaccagccaa caactgcgct
     3781 tcctgtcatt gctgaaaaac cacattcgcg attacggcac cattgaaatg cggcagctct
     3841 ttgaacagcc ttttacgcat atccacaacg aaggcgttac cggtgtgttc cctgatatag
     3901 cgcaaatcgc ccgcctgcaa aagatagtcg aagagctggg tgttgtgacc gacgcagcga
     3961 cggtataata cacgcatgac aagccccggc agcggggctt ttgtatttaa aacaggatat
     4021 aaacgctaac aatgattact ggcgacttaa aaagtaaaat cgacggacta tgggaagatt
     4081 tctgggtggg tggtatcacc aacccgctga ccgtaatcga acagatcacc tatctgatgt
     4141 attcccggat gctggatacc caggaacaac gcgacgaaaa gcgtaaacaa atcgcgggta
     4201 ttgattttaa accgcgtttt gcgccagaac agcaggagtt ccgtttcagt cactatagca
     4261 accttggctc ggatgagatg atggaagtgg tgcgcgatgg cgtattccag catttccgtc
     4321 agctcggcca ggccgatgct tcgaaggtga cgctgctggg caactttatg aaagatgctc
     4381 gtctggagat tgttaagccg tcattgctga ccaaagcggt tgaggtaatc aaaaacctgc
     4441 cattggatcg cggcgacacc aaaggcgacc tttacgaata cctgttaagc aagctgacaa
     4501 ctgccggaat caacggacag ttccgcacac cgcgccacat tatccgcacg atggttgaaa
     4561 tgatggagcc gaacccggcc cgcggcgaga cgatttgcga tcccgcctgt ggcaccggtg
     4621 gttttctggc aaccagctat gaatatctgc tggagaagta cagctcgctg gaatccattc
     4681 atactgagat tggcaccaac gaacgtggcg agctggaaga gcaaaaaatc tttaccggcg
     4741 atctgctgac accgtggcgt aaccatgtgg ataacaacat gttccacggt tacgactttg
     4801 acaccacaat gctacgtatc gccgccatga acctgattat gcacggcgtg gatgcgcctg
     4861 atatccacta tcaggacaca atgagccaga gtttcagcac aaacttcccg caggccagta
     4921 aaaacgcctt caacctgatt ctggcgaacc cgccgtttac cggttcactg gacgaggaag
     4981 atatcgactc cacgctgtcg gcaatggtga aaaccaaaaa aaccgaacta ctgttcctgg
     5041 cgcgtattct gcaaatgctg aaagtgggcg ggcgcagtgc cactatcgtg ccgcagggcg
     5101 tgctgtttgg ctctagcaag gcgcaccagt cacttcgcaa aacgctggtg gaagataacc
     5161 aactggaaac ggtgatcaat ctgccttctg gtgtatttaa accttacgct ggcgtggcga
     5221 cggcgatcct gatctttacc aaaggcggtc aaacggatga ggtctggttc tacgatctac
     5281 aaaatgacgg ctacagcctg gatgataagc gcaacccgat aaaagacaac gatctgccgc
     5341 atctgctggc aagctggaag cattaccgta ctttacgcgg gctaccggtt gataacttta
     5401 tgggtaagaa gttagcctcg ttgcttaaac agcagtaccc ggaagggatt aatgctggcg
     5461 ttgattttaa agatcgcacg caggcggcgt ttgttgtacc gaaagcggat attgctgcgc
     5521 agaaatacga tctatccatc aaccgttata aagaagtcgt gtatcaggcg gaggaatatg
     5581 aagatccgaa ggtgatattg aagcggttaa aggatctgga aaaagagatt ctggcggatt
     5641 tggatgagct ggaggggatg ctgtgagttc ggcgatggtg aagttagggg atgttattaa
     5701 tgttcactac ggaaaagcat taaaagctga tcagcgtgta tcgaatggaa gtgtccatgt
     5761 gtttggtagt tcaggaattg tcggcaacca cgataaaacg ctttgcagct atcccacgat
     5821 aattattggc cgcaaaggca gcgttggtgc tattacctgg gctccaagcg gaggctggat
     5881 cattgatact gcttattacg ttgagatcaa agataataat aagttagatc tccgatactt
     5941 gttttatatt ctgagcggaa ttgatctcac taagaaaacg attaccacaa gtattcctgg
     6001 attaaataga gacgatctct atgatacatt tattaaatta ccaccttttg aagagcagaa
     6061 acgaattgtg gatcttctcg ataaagctga gggtatacgc caaaaacgtg agcaatccat
     6121 caaactggct gatgattttt tgcgtgctac gtttgctaca atgtatggga atcctattac
     6181 aaaccccaaa aaatggcctg tccatcttat gggagaaatt attgaattta aaggtggaaa
     6241 tcaaccacct aaaagtgatt ttatttttga acctaaacag ggttatatta gattagtaca
     6301 aattagagat tttaaatccg ataaatatgc gacgtacatt ccgcaagaaa aagcaaaacg
     6361 tattttcgaa gttgatgatg ttatgattgc tcgttatggg ccacctgtgt ttcaaatact
     6421 tagaggttta tccggcagct acaatgtagc tttaatgaaa gcttcaccca aagaaaatat
     6481 taggaagggg tttatattct atcttctaca acttccggaa tatcatgatg ttgttgtaaa
     6541 aaactctgaa agaacagcag gacaaaccgg tgtaaatctt gaactattaa acaaattcaa
     6601 tgttcccctc ccacctatat actatcaaga tgagatatta gatagattag ctagaattga
     6661 aaaatttaaa gaaaaaatag aaatatcatt aaatcattta gaaatacaat ttttatcact
     6721 acagaagaga ttaataggtt tttaaatcct tagtatcgaa ataaacatta atttattata
     6781 ttaaattaag atattttagg aaattaaggc gtcactctta ggtaacgcct ttttttacta
     6841 tctcgccccc taagaaggtt atatcttact taatacctgt gactattttt gatataatca
     6901 taaatacaca ctaatttgca aaaattactt gacaacacct gagtattcat agcttcatct
     6961 atatgttttt tcaatatttt gagaagctct cccatatcgc gcttgattaa tccaggctcc
     7021 atataaggca tcattcttaa aaagaaactg aaatcataag caagatataa ctcattgacc
     7081 cccaccttga cctcaggaac ggtttcagag cgtcgtttat cccatccttg agactgaaaa
     7141 ctaataggag tgatatttaa tcgtgataaa acgccacctg agagttctgt ttcattaatt
     7201 atactcccat caggattaat taatttagcg tgatttaaga agtgaaaaat aggaatgcgc
     7261 gcattgcctt tgcatatatt tggcaaaaca tcattaacaa cacatcccca atcataatat
     7321 ccattttcaa atacaacctc tcggatgaga tctacacgct ccagtccaac caatccttta
     7381 gttgccatcg attccgattt ttttaccatc gaaatagcac catatcccac aacaaactca
     7441 gttgtactga ccccttcttc aattttctct gaatccctca catataattt tcctttcgga
     7501 tcatcagtta agaccaactc ataaagttca tcttttatca ttcggaataa atgtgctgga
     7561 atcctttttt tagtttcact cagcacctca aatatttctt tataggatgg tgcctgaaca
     7621 tattttaccg
//
LOCUS       DAJVTY010000176         7517 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVTY010000176 DAJVTY010000000
VERSION     DAJVTY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.176"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..590
                     /locus_tag="OQQ66_003548"
     CDS             <1..590
                     /locus_tag="OQQ66_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1906332.1"
                     /translation="INQLADKQTIKQTLFARQPAKIHKPYPTNSKIIMGDVLNHAALK
                     QAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYDEVPGKFGEWNN
                     AVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFKGTIVSRKS
                     VAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            900..1628
                     /gene="mpaA"
                     /locus_tag="OQQ66_003549"
     CDS             900..1628
                     /gene="mpaA"
                     /locus_tag="OQQ66_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1906333.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1603..2568)
                     /gene="ycjG"
                     /locus_tag="OQQ66_003550"
                     /pseudo
     CDS             complement(1603..2568)
                     /gene="ycjG"
                     /locus_tag="OQQ66_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            2687..3193
                     /gene="tpx"
                     /locus_tag="OQQ66_003551"
     CDS             2687..3193
                     /gene="tpx"
                     /locus_tag="OQQ66_003551"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1906334.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3237..4778)
                     /gene="tyrR"
                     /locus_tag="OQQ66_003552"
     CDS             complement(3237..4778)
                     /gene="tyrR"
                     /locus_tag="OQQ66_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1906335.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4926..5987)
                     /locus_tag="OQQ66_003553"
     CDS             complement(4926..5987)
                     /locus_tag="OQQ66_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1906336.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5984..7381)
                     /locus_tag="OQQ66_003554"
     CDS             complement(5984..7381)
                     /locus_tag="OQQ66_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1906337.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1858 a         2077 c         1807 g         1775 t
ORIGIN      
        1 tgattaatca actggcagat aaacaaacca ttaaacaaac tttatttgcc cgtcagccag
       61 caaaaatcca caaaccctat ccaactaata gcaagattat tatgggagat gtactgaatc
      121 atgcagcact gaagcaggcc atgcagggac aggacgttgt gtatgccaac cttacggggg
      181 aagatttaga tattcaggca aattcagtga tcgctgcgat gaaagcctgt gatgttaaac
      241 gtctgatctt tgttctttcc ctcggtattt atgatgaagt tccaggaaaa tttggcgaat
      301 ggaataatgc agttattggt gagcccctaa aaccattccg cagggctgcg gatgcaattg
      361 aggcttctgg actggaatat acaattcttc gccctgcctg gctgactgat gaagatatta
      421 ttgattatga gcttacctca cgcaatgaac cattcaaagg tacgatcgta tcacgtaaga
      481 gtgtcgcagc attaatcaca gatatcatcg ataaacctga gaaacacatt ggtgagaata
      541 taggtatcaa tcaacccgtg actgatggtg acaagccatt attcatgtaa atcaattaca
      601 tagttaccat cgtaatgtac tatgtgcagt acgatttatt aaacttatta aggtgaactg
      661 atcaatccga tgcgattaac atgtgtagaa ccagtaagaa taaaatacgc catgtatata
      721 acattacgac ttacatctaa gtcagcataa ttctaatatc tgcacatcaa taaaaatgca
      781 aacaacttcc ggcaaaaaaa gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt
      841 tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca gagacgatca
      901 tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc gcccggaaca gaacattacg
      961 gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc agccgcgaaa
     1021 gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc acgctctcct
     1081 gtgctctgcg gacgttgaca ccttctttgc gccgccatca tgtggtgctg tgtgtgaatc
     1141 ctgacggctg ccagctgggg ttacgggcca atgctaatgg tgtggattta aacagaaact
     1201 ttccggcggc gaactggaag gaaggtgaaa cggtttatcg ctggaacagc gccgctgaag
     1261 aacgtgatgt cgtattactg acaggtgaca aacctggctc agaacctgaa acccaggcgc
     1321 tgtgccagct tatacaccgc attcaacctg cctgggtggt ctcattccac gatccactgg
     1381 cctgtattga agatcccaga cacagcgaat taggcgaatg gctggcccag gcgtttgaat
     1441 tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt tggtgtgcag
     1501 atctcaactt gcattgcatc accgcagagt ttccgcccat ctcctccgac gaagccagcg
     1561 aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc
     1621 cgtcgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc cgtcaagatc
     1681 ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag aggtacacaa
     1741 catgcagccc agcatcagac tgaaaccttg tgcacgcgct tcagtcgcca gcgccagcgc
     1801 ttccgtcaga ccaccggttt tatcgagctt aatgttaacc atctcatagc gccctttcag
     1861 cgccttcaga ttgctacgtg tatgacaact ctcatcagca caaatcggca acggatgaat
     1921 aaaattctcc agcgccgcat cgtcctgcgc aggaaggggt tgttcaagca tcgcaacgcc
     1981 taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg attcatttgc
     2041 atcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt gccaccatcc gctcactaat
     2101 aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga gtgttgatgc
     2161 actattggcc atctgatcag gcgtaccgat gactaccgtc tgtgcagtga taactgtctc
     2221 gggaagcgtt atgccgatca aatcagccag cgattgctgc tgttttcgcg cggccagatc
     2281 ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt tttgcaactc
     2341 ctcccgtgtc agccctttct ctaattgcga cacgacgctc ataatttgcg ccattaccga
     2401 ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt taataccctc
     2461 ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccggg caatcacaaa
     2521 cggggtatgt aagggctagg cttcctcaaa taccttaacg gttctcatct ttactccttg
     2581 ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc
     2641 ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt cacaaaccgt
     2701 tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc
     2761 gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc agtttgcggg
     2821 taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt
     2881 acgtaagttt aaccaactgg caaccgagat cgacaacacc gttgtgctgt gtatttctgc
     2941 cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac
     3001 cctctccact ttccgtaacg ctgaatttct gcaagccttc ggtgtggcaa ttgctgatgg
     3061 cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt gacgaaaatg acaatgtgat
     3121 tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag ctctggctgt
     3181 actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat tcgcgcttac
     3241 tcttcgttct tcttctggct taagccatat tcccgcaact tattggcaat cgcggtatgt
     3301 gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata aagctgggtt
     3361 aataccgagc gttcaaaacg gctggtgatt tcgtccagcg aaccttccat cgcatcttcg
     3421 cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa tatcctgtgg acgcagctca
     3481 taaccgtcca gttgtgtcag tgcgcgatat atagcgttct ttaactgccg cacatttccc
     3541 ggccacgcat aacgcgtaag tacagtattc aggtcagcgg ccagtttcgg acgcggcacg
     3601 ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta acggcatgat gtcctgcgga
     3661 cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata gagatcttca
     3721 cggaacatgc ctttttgcac cagttcgacc agattcttct gcgtagcgca aatcacccgc
     3781 acatcgacat gcacctcatg gtcttcgcca acccgacgga aagtgccatc attaagaaaa
     3841 cgcagtaatt tcgcctgcat ccgtggtgac atttccccta tttcatccaa cagcaccgaa
     3901 ccaccgttcg cctgctcaaa gaatcctttc ttcccttccg gagcatgacc aaacagttca
     3961 ctctcgaccg catcttccgg tatagacgca cagttcagcg ccaggtaagg tttgcccgct
     4021 ctggggcttg cctgatggca ggcgtaggca aagagatctt tacctgtacc tgtgtcaccc
     4081 gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg cctgttcgac aacatgcttc
     4141 attttcgggc tgacggcgac aatttgactg aaggcgctga cgtcctgggc ggcgacattt
     4201 tgcaactggc ggcccatacg aatcgttgat cgcaacatca ccaccgcacc ggtcaggacg
     4261 tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa attctgccca
     4321 ttaataacga catgctcgtt atgcgaatct tgcggttcgc tttccagcca acgtaaaaaa
     4381 ttaaagccgt taatcaattg tgcggcggta tggttgcgca ggcgatccaa tttttgccca
     4441 aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc gacagagagc
     4501 acaggttcgg gcaacgcctc cagtaacgcg ctcaacgcca gatgctcacg ttcggaaggc
     4561 atccacggga cagtacgcac atcggtaaca cctgcaatac ggcgtatttc ggccatcaga
     4621 ctgctgaaac tctcaaactc cagttcagca aaattgaggt agattcgccc aatgggatca
     4681 atctcaatac cgcgtaaatc aatgcctctt agcacgagta gatcgagtaa ttcgcgggtc
     4741 agaccgagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac ctgaaaaaag
     4801 aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg tcaacaatca
     4861 ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca gcacccgata
     4921 ttgcattatt tttcgctggg cgttttgcct ttttgcagcg tttctttcac ctgaccgata
     4981 agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt caatccacgg cagcgggcgg
     5041 caaagctcca tagctttaat cccgagtcgt gccgtcagaa gtccagcacc aattccctga
     5101 gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca tccagtccat ccccacttcg
     5161 cgcaccagtt cgctggctcc ggcaaaagcg atattcagca ataccagctt aaacagacgc
     5221 aaacggctgt aataccccag ttcaatgcca tacagcgtgg cgatgcgatt aattaaacgc
     5281 agattgcgcc aggcgataaa cgccatatcg accaacgcca gcgggctgac cgcaatcatc
     5341 aacgttgatt ccgccgccga acggcagatt tcgcgccgcg cctgggcatc taaaactggc
     5401 tggaccaaat gcgcatacaa actgaccact tcacggtcgt tttgcgtttc atggattgag
     5461 gcataccagc gttgcagcgc tggatgagac tgatcaatac ccgcctgctg cgccagtttt
     5521 tcgcaaaatg cgcggccttt gcccgtgccg tggctgtgca acaaatcgcg cgcttcgtcg
     5581 cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac cacagaacct
     5641 acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgccac ccagtcctga
     5701 gtttgccagg cattcattgt ccactgtaca ccctgcccga caacgcttgc gccaaacaga
     5761 gccagcccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa cgctgcatcc
     5821 attaccgctt caacttgccc ctcttcttca ggcgcttcat cgagcgtggc cggggcaaaa
     5881 ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt ctgatcgacc
     5941 tccagcggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc aatttatctc
     6001 ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg cagcggtttg tcgacatcca
     6061 tcacctgcgg gcgaaacgct tcaaactgga acccttgttt gtcccagaac gcctgaccgg
     6121 gcaaacgcgc aggaacttca ccgggataaa cggtgagtgg cgcaccatcg ctgagccggt
     6181 taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat aatgccgctg gtggtcgcct
     6241 gcaccgaggc cagccccagg caatccatac taatcccttc aaacgccgca ttttgccagg
     6301 catcctgaat cagttgttgc agtaatgaaa ccatattggc gtgctgatcg atggtcacat
     6361 ggtccgcttt agtggcagca aacaatagct tatcgataac cggcgaaaac aaacgccgga
     6421 acaaggtacg ctgcccgtag tggaaacttt gcatcagctg cgtcagtgcc agacgcatat
     6481 cattaaatgc ctgtggccca ctgttgagag gttgcaggca atcaaccagc acaatctggc
     6541 ggtcaaagcg cagaaaatga ttcttataga accccttcac caccttctcg cagtaataat
     6601 taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc ggcctgcgcc agtttggact
     6661 cgccccaggt atcgacatcc ggccacggga agaattgcag cgcgggcgca cctgccatat
     6721 ctcctggcaa gacaaagcgc cctggctgaa taaagtgcag cccctgctgt ttacagtggt
     6781 ggagataatc ggtccacgcg gcggcaatgt ctgccagccg gttttcgtcg gcaggtgcta
     6841 gcgggtccag cccttcgcac atcattcgcc atttgaccga ccattcgccg cgctgaccat
     6901 tgagtaagcc cgtcatctgg cgtgaccagc ttaaatagtc ctgcgccagc atcggcaggt
     6961 cgagcaacca ttcgccggga taatccacaa tctccagata cagcgtggag gtgtccttga
     7021 agtggcgtag cagcgaatcg ttcgatttaa aacgtagcgc caggcggatt tcactgacgc
     7081 cgcgcgttgg cgtcggccag gcgggaggat cgccatataa ctgcgccagt ccttcatcat
     7141 atgtgaagcg cggaatgcca aagtcacgct gaggaatgcg ttttacgccc agcaggcgct
     7201 cttcacgcac cgcgcttaac agcggcaagc gtgctccggc gtgaatattg agcaactgat
     7261 tgaccatagc agtgataaac gctgttttgc cgctgcggct tagtccggtt acggcgaggc
     7321 gcagatgtct gtcgacaccc cgattcacca gcgcattaag ttcattttta agtcgcttca
     7381 ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg
     7441 gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat
     7501 gtgcagcatt cacgttc
//
LOCUS       DAJVTY010000177         7471 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVTY010000177 DAJVTY010000000
VERSION     DAJVTY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.177"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..339
                     /locus_tag="OQQ66_003555"
     CDS             <1..339
                     /locus_tag="OQQ66_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1906338.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            714..1148
                     /locus_tag="OQQ66_003556"
     CDS             714..1148
                     /locus_tag="OQQ66_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1906339.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1159..1800
                     /locus_tag="OQQ66_003557"
     CDS             1159..1800
                     /locus_tag="OQQ66_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906340.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1797..2147
                     /locus_tag="OQQ66_003558"
     CDS             1797..2147
                     /locus_tag="OQQ66_003558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906341.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRLETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2841..3452
                     /locus_tag="OQQ66_003559"
     CDS             2841..3452
                     /locus_tag="OQQ66_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906342.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELPVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3990..4532
                     /locus_tag="OQQ66_003560"
     CDS             3990..4532
                     /locus_tag="OQQ66_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCS1906343.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4525..4884)
                     /locus_tag="OQQ66_003561"
     CDS             complement(4525..4884)
                     /locus_tag="OQQ66_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906344.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5316..6140
                     /gene="dinD"
                     /locus_tag="OQQ66_003562"
     CDS             5316..6140
                     /gene="dinD"
                     /locus_tag="OQQ66_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1906345.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6432..7049
                     /locus_tag="OQQ66_003563"
     CDS             6432..7049
                     /locus_tag="OQQ66_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1906346.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7046..7471)
                     /gene="ligB"
                     /locus_tag="OQQ66_003564"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7046..7471)
                     /gene="ligB"
                     /locus_tag="OQQ66_003564"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1997 a         1836 c         1909 g         1729 t
ORIGIN      
        1 cagacggagg cgtttttgaa tgcctatggg ctgagtcgat tcgcgccgct gggttatgac
       61 ccgcgcgatt tacccatccg tgatttagcc gggtacagga agaaggggaa ccacgacggc
      121 gacccgataa ttttctacac cttcccggca gcgtttgagc aggagattgc aaagggcttc
      181 aataccaaac agtttgccga agttctaaag aatgcaggaa tgcttacacc accaacgagc
      241 gggcgaggct atcaggggcg agtaagggag gatgggcgac agattcgcgt ctatgtactc
      301 aatttcatgg cagaggaaag cagccagcca gaagaataat tatttcttca catgcgtagt
      361 tttagtgttg tctcggttgt ctttgttgtc tcaatttgta attgagattg atttataagg
      421 attttatttt aatttctgag acaacaccga gacaacattt agccattttg agacaacagg
      481 gcgaaagtgt gtttttagtc tggcaggcag tgagacaaca tttatgccgg gtgagacaac
      541 acgaaattgc aaatgttgtc tcagcaaaac ccagacgtgg cgcggcctgt agagccgtga
      601 gacaacaaag acaacagaga caacactatt tttgcttact atgaaaaaaa atcggcaggt
      661 gtcccacatt aagaaatcgt cattagcaaa actaataatc cagtgagggg aaaatgacag
      721 cacaaatttc agcatacggg cggctggtgg cagatcccca gacccgcacc acaacgaacg
      781 gtaacaacat ggctatggcg cggctggcgg tgtctctccc ctgtaatgcg gcagaggcgg
      841 gagagtcaac cttctggctg ggcgttattg cttttgggaa gcaggccgat gcgctggcaa
      901 agcaccagaa aggcgacctt gtgagcgtgg caggcaatat gcaactcaac cagtggacag
      961 ggcaggacgg cggtacacag caggggtatc aggtagtagc cgacagtgtg atcagcgcca
     1021 gaacggtacg ccccggcggg aaggcaggac agcggggaca ggcaacggac gcgctgagac
     1081 gtgcgcagca gccatcccgt gatgaatacg accaacgccc accattcgac gatgaaacgc
     1141 cattctgaga gggaagatat gagtacgcaa cccatcatta ttgagttcgg cagctatcgc
     1201 tggcccagac atcttgcaat acatgaagca gatcatgcga tagccgcatg gtttttcggc
     1261 atacaggagg tagaaattgc gttatcgggt aataaccgag tggcaaaaac ctaccttagc
     1321 ggcgatgttg atgaatgtgg agctgtcgcg gtatggcacg ccaactatcc acggaatgtg
     1381 gctgaggtta agtcttacac cctctcgaat gatgacacca ggttacgtct gatagaccag
     1441 gtatgccggg ctatggttat ctctctggct ggtatcgtcg cgcaatcacg ctatacgggg
     1501 gatgatatta acgcactgat ggaaacgtcc gggaaagcag acatgaaaaa tgttcgtgat
     1561 ttatcagagg tataccgggt ggctggtggg gaggatgctg atatacagag aatatccctt
     1621 tcccgtgctg aggcgctgat tgccctgaaa tggtgggatg ttatcgcgct ggcgaaaata
     1681 ctggagaacc gtaaccatat gcctgccagc aacttccagc gcattatggg tgatattgac
     1741 aagcctgtac caaagcctat gacgctggct gaactggatg aattggtggg tgaagaatga
     1801 agccatacat tctggcagcc ggcctgatct cctcggcacc ggcactatct gctatccatt
     1861 gtgggggctt taccatacag ccagaccgtt atcggacaac gattaacggc gatgtggttc
     1921 atattgttga tgcgcaattt tacggcgcac cgcgagactt tagccaggcc gccataaagt
     1981 tattgccgga tgccattacc actaccgata ggcaataccg cctagagacg cagggaggca
     2041 atgccttgct tgagttgatg accagagaaa acccgccacg tgtgcttaat cgtgagcgct
     2101 gcgatgccgg gttaacaagt ttcggttggt acacttctgc gccgtgatgt tcgttccgag
     2161 cgcagcctaa aatattggac tgttcttgac ggatttaaaa ctctaatgga gccaagtcct
     2221 tgccagtagc gggctaggag cctaattgtg attcaggtcg tcaaaaatat tcaggtttgc
     2281 ggcattggtt cgcagtacgc agtccggtac gcggcaaaac tgcgcattta acgtgcgtac
     2341 cacctgacga attctaacgc cggcattgca aacagatgcg tgcgcaaaat ggctaagacg
     2401 tctgatattg ctggctttgg ctcaatcagt ttggaggtgg cggtatcagt acgatgctaa
     2461 tttttgctaa ggttttagta cgcaactcag gtacgcactc tcaccagata cgaaaccgat
     2521 tcgcagttgc gtaccagcct gtaaggcgca ttatcgctgg gtttctacag tcgaatgcag
     2581 tacgcaaagt ggaaaatcct ccgaatacga aacccgagca ttgcgcagta aattgcgaat
     2641 tgcgcagtta acaaaatatc tccgcaaaac gaaacggata tcaatgcgca aaccatcctc
     2701 aacccccgcg cggttactgg ctttattgat gtgaaattgc aaaattggtg cgcaatttac
     2761 agtaaaatac gcatattatt tcgtattcaa tttgcgcacc gttcgcagtt aatgcgcagt
     2821 aattcgcagg aggccagcca gtgagagaca gaacgcgcag ctataccacc gacctgcccc
     2881 gtatagggtt gccattcctt gcgaacatgc gtagcaggct ggcggatgct gaaccgggta
     2941 cgcagcttta tacccagacc gaaagcggaa cgttgtatgc cttccggcaa gccgacagct
     3001 acgccatgac aatcaacggc gtgacgcggg ctattcgcac cacaaccacg aaggccgggt
     3061 atggcgtccg ggaatggtac gcttgccccc actgtatgaa gcgggctgca aagctgtata
     3121 tcggcaagaa agacattggc tgccgggcgt gctggaaact tcactataaa agccagagtg
     3181 ccgacatact ggacaggatg cgcatgaaga tacgccagca gcgatgcgcg atatggggca
     3241 gcaatgacct tgccaataat ctgtttaatg acattcgcat gttcccaaaa ccaaagggga
     3301 tgcgctgggc tacgtttgac aggaagcggg cagagttacc agttatggag atggcttact
     3361 ggcaggcatt cagcccggta gtggacagaa tcacgggcgt tattgagcgt aaagcaggaa
     3421 aagcagccag aggcattggc ctgactctat aaaagcaaaa cgcccgcacg gggcgtcaga
     3481 gaacgggata gacttctgcg acaatttgca ttcgccgcca atatagcaag ccgtccacat
     3541 caagcaataa actatcaccg ggagggggag gccaaatctc tgcaatcctg agccttaagg
     3601 actgcccgcc ccatcgtttt ttcatacccg cgaaaaatga aatttaatcc gggcgtgttt
     3661 catccttcca gagggtatga cgtatgacac cacgcgaaat agccttattg accatcgcca
     3721 aacttgagca tggaggccac cagcttacac aggcagatca acgggagata gaacgctcag
     3781 ttaatgccga tatagcccgg cgcgacaggt tccgcgaaat gatgcgagca cctgcctacc
     3841 agtggaagaa gccagcgccg cgcaggtaga tacgggatta ttccgcatgt aacttatgcg
     3901 tattaagcaa atttccttta ttcaccttta cagaattgtg agtacgtcag tatcgttatc
     3961 cttccgattc gcatttattc aggatttgca tgtaccagct aaagataacc atccgagaca
     4021 gcaaaccacc tatatggcgg cgtgtgctgg ttccagagca aatccccttt agtgaacttc
     4081 acgcggtgat ccagttggct tttggctgga atgacgaaca cctgtatatg ttcgagaaag
     4141 ggcgtaaagg tgatcccggt agcgagtatc gcgtatgggg tgaggatgaa agcatgggta
     4201 atgcggcaat cacaccacta tgggcagcgc tccagaatga tggtgacaag ctggtttaca
     4261 cgtatgactt tggcgactgg tgggattgcg tcattgtgct ggagaagcaa acacacgata
     4321 cgagcaatca gcccattagc tgcctgcgtg ggaaaggcac caccccggtg gaaaactcag
     4381 gcggcttgca tggttacaat gaattgctat tacaggccag agagtctgat aatcctgagc
     4441 aggccgagat ccacaatttc ctgatgctgg atattgagcg ccgggtttac gacctgagca
     4501 gcatcaacga cagattgcag actcttaact gacccgcata tattcgcctg atattgcgtt
     4561 atcagcgccg ccagtatcgc gcataacagc gcactgaccg ggcaaaccgt agcgcacgac
     4621 cgttgcgtta gtctcaccgc cgcgccgtat ctggcgcgat tcatacactg tcacccgctc
     4681 aagcagtcca ccagcaacca tgctttccag tgtgcgccgg gttgattcga gctggtgacg
     4741 cttatcgaac gacaccatgc catgaagcag gtaggccaca cccgacacat cgaacggcgg
     4801 cgcaccaatc tcaccagtca cccattcgag gttatccggt tcaaagtagc taagtatctc
     4861 ttttttgcgg ctggtcattc tcatagctgg caggttcctt attgtgggat agcactatca
     4921 tacaataagg cgcaaaagat aagaggccag aacagcaagc atgagagcat aaaacgagca
     4981 gccaaagaca gcgcctcaaa ataccgtgta cacaagggtg tacctactga gaaaacgcat
     5041 tacaagaaac acagcaacca cgggcgttta aagccactac tcaataaaca gattcaacgt
     5101 aacgccaatt cttacttttc agccttcaca aatgccgccc actcaaacag agcggcattt
     5161 ttcttccccg caacttcaat tctgttttgc gtgcctgctc cagattttgc gatgtttttt
     5221 tgcccagcac actgagaacg tgagatactc acaactgtat ataaatacag ttacagattt
     5281 actttctttg caatcgatat cacatggagt gggcaatgaa cgaacatcat caaccttttg
     5341 aagaaatacg gcattatggc actgaagggc aagaattttg gtcagctaga gaattggcac
     5401 cgttactgga ttatcgggac tggcgcaatt ttcaaaaggt actggcccgc gcgactcaag
     5461 cgtgcgaagc cagcaatcaa gctgcatctg accatttcgt tgaaaccacc aaaatggttg
     5521 tattaggttc aggggctcaa cgagaacttg aagatgttca tctctcccgc tatgcctgtt
     5581 acctggtagt acaaaacggc gaccctgcga aaccggttat tgcggcaggg caaacttatt
     5641 ttgctatcca gacccgacgg caggagctgg ctgacgatga agctttcagg caacttcgtg
     5701 aagacgaaaa gcgtcttttt ctgagaaatg aattgaaaga acataacaaa caattggttg
     5761 aggcagcgca gcaagctggg gtagcgacag ctatcgattt cgccatattt cagaatcatg
     5821 gttaccgggg gctgtatggt ggattagatc agaaagctat tcatcagcgg aaggggctga
     5881 aaaagaatca gaatatcctg gatcatatgg gttcaacaga actggcggct aatctctttc
     5941 gagctaccca aacagaagaa aaactcaagc gggatggcgt gaattcaaaa cagcaagcaa
     6001 acaccacaca ctttgacgtg ggtcgcaagg tgaggcaaac cattcaggaa cttggcggaa
     6061 ccatgcctga ggagttgccg accccgcagg tcagcatcaa gcagttggaa aacagtgtaa
     6121 aaattacaga gaagaaatag cagaaactaa aaactctttt tcttaattga gatacccgat
     6181 gcgtaagcca aagtccaggt gcagtgaaca caatggctaa acattgcacc tttcttttcc
     6241 acccctcaat tttaacctat tttttcttat gcattttctc agacaagaac tcagaataat
     6301 gcatctacgc tacagaaaat agcgatttca cataactaca agttatcaat ttcctcttac
     6361 cttaaaaaat ctcaatcgtg acaatgcgca caaatcgcta ccctgccaga cagattttta
     6421 gggagagaac catgctgtta cacattttgt atttggttgg catcactgcc gaagccatga
     6481 cgggggcgct ggcggccgga cgacggcgca tggatacatt tggcgtaatt attattgcga
     6541 ccgccaccgc aattggcgga gggtcagtgc gcgatattct gctgggccac tatccgctcg
     6601 gctgggtcaa acacccggaa tatgtgatta tcgtcgctac cgccgcagta ctcaccacta
     6661 tcgttgctcc tgtaatgcct tacctgcgca aagtgtttct ggtgctcgat gcgctcggac
     6721 tggtggtctt ttctatcatc ggcgcacagg ttgcactgga tatggggcac ggcccaatta
     6781 ttgccgttgt cgcggcggta accaccggcg tgtttggcgg cgttttgcgc gatatgttct
     6841 gtaaacgcat cccactggta ttccagaaag agttgtatgc tggtgtctca tttgcctccg
     6901 ccgtgctgta catcgcgctg caacactatg tttctaacca tgatgtggtg attatctcca
     6961 ccctggtatt cggctttttt gcccgcttac tggcgctacg tcttaagctg ggattaccgg
     7021 tcttttacta cagccacgaa ggtcactaag gttcaaaacc tgtgatctgc tgggcagcca
     7081 gccaactgcc cagcttcttg atttgcgcat tttccttcca ttcaataacc tgtctggcgc
     7141 gcccagatcc agtccccggc agctgctgtc agaactgctc cgtgctaaat aaaagttgcg
     7201 actaggaccg ttcatcactg gcattaagcg ccgcccgggt tagcggtatt cccattgcca
     7261 tcacccagcg agtaaaaggc tgcttacgag ccagattaaa ctgatgccat aactgcgcac
     7321 ttttactttt cgcgatcccc ggcgtgttct gtaattgctc tggcgttaat aaaagccaag
     7381 aaaagatatg ctcaaagcga tgagtctgat gcagcgcgcg ccaaccggcc tcaccaatgc
     7441 catccagccc aagaacctgt tttgacccca g
//
LOCUS       DAJVTY010000178         7442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVTY010000178 DAJVTY010000000
VERSION     DAJVTY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.178"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..232)
                     /locus_tag="OQQ66_003565"
                     /pseudo
     CDS             complement(1..232)
                     /locus_tag="OQQ66_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            229..1233
                     /locus_tag="OQQ66_003566"
                     /pseudo
     CDS             229..1233
                     /locus_tag="OQQ66_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1594..5451
                     /gene="sigA"
                     /locus_tag="OQQ66_003567"
     CDS             1594..5451
                     /gene="sigA"
                     /locus_tag="OQQ66_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1906347.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLSDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            5498..6080
                     /locus_tag="OQQ66_003568"
                     /pseudo
     CDS             5498..6080
                     /locus_tag="OQQ66_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6483..6883)
                     /locus_tag="OQQ66_003569"
                     /pseudo
     CDS             complement(6483..6883)
                     /locus_tag="OQQ66_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6887..7442
                     /locus_tag="OQQ66_003570"
                     /pseudo
     CDS             6887..7442
                     /locus_tag="OQQ66_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2180 a         1683 c         1741 g         1838 t
ORIGIN      
        1 gacgacattt cttcgataac gtgtgttctt tctcctgcat cagcttatac agggaagtca
       61 ggagcggtct actctgcatt tgcctgaccg ccaggcgttc agacaccggt aacccgcgta
      121 tatcgtgctc tatggcgtac agttcaccga ttagtttcag agcttcttcc gccgtcgcgc
      181 ttttagtact gatatataca tcgtggattt tgcgtcgcgc atgagcccag catcctgctt
      241 ccgtccgtcg gcgcgcagct ctccaatccg gataatatca ccgacaacgc cgaccggatc
      301 agtgatgagt ggaaggcctt cgaccgcgcc atcgaaagta accacggtgt tcctgaggct
      361 gccgcacgcc tgaaagaaca cctccgggac ttccgtaaca cacatgccgc cgcacagctg
      421 ggcgtttctg ctgtggcggc ccttcccggt gatcgcctct ctgccgcact gatggttaaa
      481 tcacacggca ccgtcagtgt cgacggtaaa gtctctgatg ctgacctcac ttacctggaa
      541 gaggtggcaa acagcaccgg gcaggaggtg gacaaaagcc gcctgacatc acaggccttt
      601 gcccgcgcgg cactgatcac tgatgtgggt attgctctgg ctacggaact ggaaacggcc
      661 gggcagaaat ggtctctggg cttccccccc aaattccagc gcgtcgacct gttcaactac
      721 aacgtgctgg tcagaaatta tgacagcagc gccttcaaag gcgaccgcta ccacaacacg
      781 aaaaatggca tcaacgccga catcggtgcc agtacgaacc tggatgacaa ctggacgctg
      841 ggactggtcg cacagaacct gatcccccgt agtattgaga caaaagaagt gaacggtatc
      901 acggaaacct tcaggatccg cccgcaggtg acggccggtg tctcctggca caacgcgatg
      961 ttcaccaccg catttgatgt ggatctgacc ccggccagcg gtttcacctc cgacagcaac
     1021 cgtcagtttg ccgccattgg cgcagaattt aatgcctgga aatgggcaca gctgcgcgcc
     1081 ggttaccgtc agaatctggc cggtaacgac ggcagtgcat tcacggccgg ggtggggatc
     1141 tcaccgtttg atgtggttca ccttgatgtt gcaggcctga tcggcacgga caacacttac
     1201 ggtgcggtcg cacagttcca gttcaccttc tgagttccac ctgcaaaaca gcatgcacat
     1261 tatcgctgca tgttgtttcc atcatcaaaa gtctgtcgga catcgacaga cttaactccc
     1321 ccggataata cagaaaacct gtaaaggatt gaccggaaac aacaaagatc aaattttgat
     1381 cgcaaaggtc aaaatacaaa caccaggtga tcaatatctg accaatcaat cacttgtgct
     1441 tatctttttt ttcattttgt tacatctgtc atacaaatat aactgacagt gattatcatt
     1501 atacccttat cagttacgta ccatgactga tagttccccg ttgtaattaa atgctatccc
     1561 ataaccacaa ctcagaaata tcggagttca cgtatgaata aaatttattc actgaaatat
     1621 agtcatatta caggtggatt agttgctgtt tctgaactga cccggaaagt tagtgtcggt
     1681 acatcaagaa agaaagttat cctcggtatt attttatcct caatatatgg aagttatggt
     1741 gaaacagcat ttgcagcaat gctggatata aataatatat ggacccgcga ttatcttgac
     1801 cttgctcaaa acagaggaga gttcagaccg ggtgcaacaa atgttcaatt aatgatgaaa
     1861 gatggaaaga tatttcattt tccagaacta cctgtacctg atttttctgc tgtttccaac
     1921 aaaggtgcaa caacatcaat tggaggtgcg tacagtgtta ctgcgactca taacggtaca
     1981 cagcatcatg caataacaac acagtcatgg gatcagacag catataaagc aagtaacaga
     2041 gtatcatctg gcgacttttc ggttcatcgt ctgaataaat tcgtcgtgga aacaacaggg
     2101 gttacggaga gtgccgactt ctcactttct cccgaagatg cgatgaaaag atatggcgta
     2161 aactacaacg gtaaggaaca aataattggc ttcagagcag gtgccggaac aacctcaacg
     2221 atattaaacg gcaaacaata tctgtttgga caaaactata atcccgactt gttaagcgca
     2281 agtcttttta atctggactg gaaaaacaag agttacattt ataccaacag aacccctttt
     2341 aaaaactcac caatttttgg cgatagtggt tctggttctt atctatatga taaagaacaa
     2401 caaaaatggg ttttccatgg tgttaccagt acagttggtt ttatcagtag taccaatata
     2461 gcctggacaa actactcgtt atttaataat attctggtaa acaatttaaa aaagaatttc
     2521 acaaacacta tgcagctgga tggtaaaaaa caagagttat catcgattat aaaagataag
     2581 gacctgtctg tctcaggagg aggggaatta acgctcaagc aggataccga tcttggcatt
     2641 ggcgggctta tattcgataa gaaccagaca tataaagtgt acggaaaaga taagtcttat
     2701 aaaggtgccg ggatagatat tgataataat accaccgttg aatggaatgt taagggcgtt
     2761 gccggagata atctgcataa aataggtagt ggtactctgg atgtaaaaat agcacaggga
     2821 aataacctta aaataggtaa tgggactgtc atccttagtg ctgaaaaagc cttcaataaa
     2881 atttacatgg ccggaggtaa aggtacggta aaaataaatg ccaaagacgc tttaagcgaa
     2941 agcggtaatg gcgaaatcta ttttaccaga aatggcggaa cactggatct aaacggctat
     3001 gaccagtcat ttcagaaaat cgcagcaaca gatgcgggaa caaccgtaac gaactcaaac
     3061 gtgaagcaat caacattatc acttactaat actgatgcat atatgtacca tgggaatgta
     3121 tcaggtaata taagcataaa tcatattatc aatactaccc agcaacataa caataatgcc
     3181 aatctgatct ttgatggctc agtcgatatc aaaaacgata tctctgtccg gaatgcacag
     3241 ttaacattac aaggacatgc gacagaacat gccatattta aagaaggcaa taacaactgt
     3301 ccaattcctt ttttatgtca aaaagactat tctgctgcca taaaggacca ggaaagcact
     3361 gtaaataaac gttacaatac ggaatataag tccaacaatc agatagcctc tttttcccag
     3421 cccgactggg aaagtcgtaa atttaatttc cggaaattaa atttagaaaa cgcaaccctg
     3481 agtataggcc gggatgctaa tgtaaaagga cacatagagg ctaaaaactc tcaaattgtt
     3541 ctgggaaata aaactgcata cattgacatg ttctcaggaa gaaacattac tggcgaaggt
     3601 tttggattca gacaacagct tcgctccggg gattcagcag gcgaaagtag tttcaacggc
     3661 agtctgagtg ctcaaaacag caaaataact gttggtgata aatcaactgt tactatgact
     3721 ggtgcattat ccttaattaa tacagacctg attatcaaca aaggagctac tgttaccgcc
     3781 cagggaaaaa tgtatgtaga taaagctatt gaactggccg gaaccctgac attaacaggc
     3841 acccctacag aaaataataa atacagcccg gcaatctata tgtcagatgg atataatatg
     3901 acagaagatg gtgccacgtt aaaggctcaa aattatgcct gggtcaatgg taatataaaa
     3961 tcagacaaaa aagcatctat tctgtttggt gttgaccagt ataaagaaga taacctggac
     4021 aaaaccacac acacaccgct ggctacaggt ttgctgggtg gctttgatac ttcttatacc
     4081 ggaggtattg atgctcctgc tgcctcagcc agcatgtata acaccttatg gagagtaaac
     4141 ggacagtcag ccctgcaatc attaaaaacc cgcgacagtc ttttgttgtt tagtaacata
     4201 gagaattcgg gtttccatac tgtgacagta aacacactgg atgccactaa tactgctgtg
     4261 attatgcggg ctgatctgag ccagtctgta aatcaatcgg ataaactcat tgttaaaaat
     4321 cagttaaccg gaagcaataa cagtctgtcg gtcgatatac agaaagtggg aaataataac
     4381 tcaggattaa acgttgacct gataacagcc ccaaaaggaa gcaataaaga gatatttaaa
     4441 gccagtactc aggccatagg tttcagcaac atatctcctg tgatcagcac gaaagaggat
     4501 caggaacata ccacgtggac cctgaccgga tataaggtgg ctgaaaatac agcatcttcc
     4561 agtgcagcaa aatcgtatat gtccggtaat tacaaagcct tcctgacaga agtcaacaac
     4621 ctgaataaac gaatggggga tctgcgtgac accaatggcg aggtcggtgc atgggcccgc
     4681 atcatgagcg gagcaggttc agcttctggt ggatacagtg acaactacac ccatgtgcag
     4741 attggtgtgg ataaaaaaca tgagctggat ggacttgacc ttttcactgg tctgactatg
     4801 acgtataccg acagtcatgc cagcagtaat gcattcagtg gcaagacgaa gtccgtcggg
     4861 gcaggtctgt atgcttccgc tatatttgac tctggtgcct atatcgacct gattagtaag
     4921 tatgttcacc atgataatga gtactcagcg acctttgctg ggctcggaac aaaagactac
     4981 agttctcatt ccttgtatgt gggtgctgaa gcaggctacc gctatcatgt aacagaagac
     5041 tcctggattg agccgcaggc agaactggtt tatggggccg tatcaggtaa acggttcgac
     5101 tggcaggatc gcggaatgag cgtgaccatg aaggataagg actttaatcc gctgattggg
     5161 cgtaccggtg ttgatgtggg taaatccttc tccggtaagg actggaaagt cacagcccgc
     5221 gccggccttg gctaccagtt tgacctgttt gccaacggtg aaaccgtact gagtgatgcg
     5281 tccggtgaga aacgtatcaa aggtgaaaaa gacggtcgta ttctcatgaa tgttggtctc
     5341 aacgccgaaa ttcgcgataa tcttcgcttt ggtcttgagt ttgagaaatc ggcatttggt
     5401 aaatacaacg tggataacgc gatcaacgcc aacttccgtt actctttctg ataacagccc
     5461 gggccgcgtt tgcggccctt cttctaccgg agagaatatg tattaccctg tgacagacta
     5521 tatcgctctt gctctcatta ttagctttct ttttctgaca ttatttatct gcctgttatg
     5581 ccttaaacat gagcgaataa aaaaagaaac tatcaggcaa aaaaaatgca catattctgg
     5641 agcatggctg gaatgcaact gagttctcat ggttccgata cggacagtat aacgaaacgg
     5701 gtatttacat ctcgatcgag aagaccatta tcattacaat caccgtttct ggtgggtgct
     5761 ttaaaaaaga atacagcatt gtctcccaca tgctggtcac tgacaccata acagaggcca
     5821 ccctgtacga aaacgggctg tatacgcgcc acatcagact cagtcgccca gtatctgaca
     5881 gcaaataccc cttgccaccc ggatctcagc tcattaaaaa tatgacctta cgactgcgcc
     5941 tgcaggatca gcaggaagaa acatccgtga cattatttca gggaaaaatg agtaccgacg
     6001 gaaataacta ttacatcatc aaaggtaaag taagctcagt acttctgtta ttaaaaatgc
     6061 tgcagattaa tcacgcctga tctctgtccc ggtattttag cccgaggtat attctgaaat
     6121 atcattcatc atgaccggcg tttatacaaa cgccggtcat tcgctaacag cttcaccgtt
     6181 ccttccccca tccaggttcc gcatgagcgt gttgtaatgt tcataagcgt attatcgtaa
     6241 gcttccgggg aactcatatt gacgcgtttt tcaggcgcgc gtcatcggcg gcgcatacga
     6301 acgatcgtgg tttgataacg gttgatttat tttgttgcac aggggcgatc gctaggcagg
     6361 taccggaggc gggctctgaa gtactgagta gggtgatcgc gtcaggccgc gaagtactga
     6421 ctcggggatt aatagcaggt gatgagtaag cgtacagcga gggccgtatt gacggggatg
     6481 tgttattcag ctggcagtgc tatgcgccac ggaagcagtt cgctgacccg gttgaccggc
     6541 cagtctgcta tgacgccaag cacatggcga aggtagcttt ctggatccac gtcattcagt
     6601 ttgcacgtcc cgatcaggct gtacagtagc gctccccgct caccaccatg gtcagagccg
     6661 aagaacagga agtttttacg acccagactg accgcccgca aggcattttc agcgatgttg
     6721 ttgtcgattt ccacccagcc atcgttcgca tagtacgtca gtgccggcca ctggttaagt
     6781 gcgtacgcga acgccttcgc caactctgag tgtcgcgaca gggtcttcat cttttccgca
     6841 accagctttc cagggatttc aacagcggtt tcgtttttcg ctgacaccat ggagggagtg
     6901 gttaaaagag caacgagaaa gtggtaatta taacgccagc cggatatggc gggaaatggt
     6961 ggcccgggga tttacaggca gtgaaaccat cgtcagggat gcggttgcca aatggcgtaa
     7021 aggctggaac ccaccggtta ctactgccgt cagacttcct tcagtgtccc gggtaagccg
     7081 ctggttgatg ccctggagaa taaccaggga tgaagaaaat tatgcttccc gatttattag
     7141 tctgatgtgt gaaaaagaac ctgagctgaa aatagcgcag caactggcac tcgagttcta
     7201 ccgtattctg aaaacccaaa ataaatcaca gctaagcagt tggttcactc gagtccacga
     7261 aagcggttca gcagaatttc ggcgcgtggc ggcggggatg gaagctgatg ctgcagctat
     7321 atgtgaggca atcagtagtc gctggagtaa tggtgttgtc gaaggtcatg taaatcgcct
     7381 gaagaagatg ctgaaacgcc agatgtatgg tcgagcagga tttgaactac tcaggcagag
     7441 gg
//
LOCUS       DAJVTY010000179         7388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVTY010000179 DAJVTY010000000
VERSION     DAJVTY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.179"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..1459
                     /locus_tag="OQQ66_003571"
                     /pseudo
     CDS             1..1459
                     /locus_tag="OQQ66_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1470..2543
                     /locus_tag="OQQ66_003572"
     CDS             1470..2543
                     /locus_tag="OQQ66_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1906348.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMANVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKLGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2791..3831
                     /gene="pstS"
                     /locus_tag="OQQ66_003573"
     CDS             2791..3831
                     /gene="pstS"
                     /locus_tag="OQQ66_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1906349.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3918..4877
                     /gene="pstC"
                     /locus_tag="OQQ66_003574"
     CDS             3918..4877
                     /gene="pstC"
                     /locus_tag="OQQ66_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1906350.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4877..5767
                     /gene="pstA"
                     /locus_tag="OQQ66_003575"
     CDS             4877..5767
                     /gene="pstA"
                     /locus_tag="OQQ66_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1906351.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5858..6631
                     /gene="pstB"
                     /locus_tag="OQQ66_003576"
     CDS             5858..6631
                     /gene="pstB"
                     /locus_tag="OQQ66_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1906352.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6646..7371
                     /gene="phoU"
                     /locus_tag="OQQ66_003577"
     CDS             6646..7371
                     /gene="phoU"
                     /locus_tag="OQQ66_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1906353.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1855 a         1717 c         1930 g         1886 t
ORIGIN      
        1 cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga cggatttaca ccggacgtcc
       61 ttcaggcgca aatacttcat ggtttttccc acgggatcac tttatatgga ggtatgcagg
      121 ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa agatcttggc gctttgggcg
      181 caatttcttt cgatgtgaca catgctcgtg cgaattttag ccatgatgat acagaaacgg
      241 gtcagtcata tcgctttctc tattcaaaac gatttgacga cacagacact agcttgcgcc
      301 tggttggcta tcgttactcc accgagggct actataccct caatgagtgg gcatcgcggc
      361 gcaacagccc tgaagacttt tgggaaacag gtaaccgacg tagtcgcgtg gagggaacgc
      421 taacgcagtc gttggggaga gattatggca atttatacct gacattaagc cggcaacaat
      481 actggcatac cgatgatgtc gaacgattaa tgcaatttgg ctacagcagt agctggaagc
      541 gtctctcgtg gaacgtctcc tggagttatt ccaatactgc cagacagggg acggggaaca
      601 accatgccag tgataacacc agtgagcaga tctacatgct ctctttatct gttcctttat
      661 cgggctggtg gggtaatagt tacgccacct attctgtttc gcaaaacgat aattccggta
      721 gctcacatca agcggtacgg cgctggaaag aaataacctt tcatggaatt taatgcagtc
      781 ctataacagt catgatgatg aggttggcgg taatatgtcc ctgacctatg atggctctta
      841 tggcacggtg aacggcagct ataactacag ccaaaattcc cagaggctga attatggtat
      901 cagaggggga attctggcac acagcgaagg ggtaacgtta agtcaggagt taggtgaaac
      961 tattgctctt gttaaagcac ctggggccgc cgggttagaa atagataata tgcgcggtgc
     1021 tgcgacggac tggcgcggct atacggtcaa gacacagcta aacccttatg atgaaaatcg
     1081 ggtagcaatc agcgataact atttctcgaa gtcgaatata gaacttgata ataccgtcgt
     1141 tacgatggtt cccacgcgtg gtgcagtggt taaagcggag tttgtgactc atgtgggtta
     1201 tcgcgttctc ttcagagtgt taaatgcaaa tggtaaaccg gtaccttttg gagccattgc
     1261 tgcgatacaa gatgcaagtt tggcagattc aggaattgtc ggtgaccgtg gcgaacttta
     1321 tctttctggt ctaccagaaa aaggacaggt tacgttatcc tggggagaaa acgcctcaac
     1381 aaaatgcatc ttcaattatt cactttcgac accagaaagt gagagcggat taattgaaca
     1441 gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag tggtgctttg
     1501 ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt atctgtaccc
     1561 ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt accgcgaacg
     1621 aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca tatcctggac
     1681 gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat acgtcaaaaa
     1741 taaaccaggc attaacttat gctggggttc actctaatat taattattat gatcttaatc
     1801 cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt
     1861 ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac
     1921 ctttaagtat atctagtggg gctaaagcga tagtatattt ttatatcaag aagacatttg
     1981 caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg
     2041 acaccccggt ggattactca cagccgatgg ctaatgttta tattcggggc gatattacag
     2101 ctccgcaaag ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac
     2161 cagctgcaga tttttcatct gttgttggaa gtgcggtaat aacacataaa attactaaaa
     2221 cggtcactat tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt
     2281 tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg
     2341 ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa
     2401 cagacatggg taaatcaaca gtgtatggtg aaaaattagg tagtgtaact ttttcagctg
     2461 ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga
     2521 taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat
     2581 ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt
     2641 tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca taaaaatgtc
     2701 atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac
     2761 aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact
     2821 gttgtcgccg cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca
     2881 ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa
     2941 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
     3001 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
     3061 caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt
     3121 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
     3181 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
     3241 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
     3301 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
     3361 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
     3421 cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa caacctggcg
     3481 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
     3541 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
     3601 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
     3661 aaaccagaac aaggcacaga agtgctaaaa ttcttcgact gggcgtacaa aaccggggct
     3721 aaacaggcga acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc
     3781 gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactcc
     3841 aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt
     3901 taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg
     3961 gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg
     4021 gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg
     4081 ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga
     4141 tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg
     4201 gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg
     4261 ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct
     4321 ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc
     4381 cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg
     4441 tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac
     4501 aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta
     4561 tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc
     4621 tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc
     4681 tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat
     4741 ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt
     4801 ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga
     4861 atgagggggc acgctaatgg ctatggttga aatccaaacc actgcggcgc tggctgaatc
     4921 tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat
     4981 ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg
     5041 cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga
     5101 aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt
     5161 attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc
     5221 ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt
     5281 ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct ctggctgggc
     5341 gggcgtgatt gccctggcgc tgttgcaggt gccgattgtt atccgcacca ccgagaacat
     5401 gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg
     5461 gaagatgatc tctgcgatta cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct
     5521 gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa
     5581 ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa
     5641 gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg tattgattat
     5701 taccctgtgc gtactgctgc tgaacattct agcgcgcgtt gtttttgcga agaataaaca
     5761 cggttaacac tttgccggat gcggcgtgaa cgccttatcc ggcctacggt aagcctgatt
     5821 agcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc
     5881 gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa
     5941 catcaacctg gatatcgcta aaaaccaggt tacggcgttt atcgggccgt ccggctgcgg
     6001 taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg agcagcgtgc
     6061 ggaaggtgaa attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact
     6121 gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgtttccga tgtccatcta
     6181 cgacaacatc gcttttggcg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga
     6241 gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca
     6301 ccagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat
     6361 cgccattcgc ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accctatctc
     6421 taccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt
     6481 cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg
     6541 cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac
     6601 agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat
     6661 cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg
     6721 atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac
     6781 caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa
     6841 gtggcgatcg atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac
     6901 ctgcgactgg ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg
     6961 gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg
     7021 gtaagtctgg agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg
     7081 ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat
     7141 caggaatacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc
     7201 attccgagcg tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc
     7261 tgccagaata tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta
     7321 ggcggtgatg agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga
     7381 caaatccc
//
LOCUS       DAJVTY010000180         7327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVTY010000180 DAJVTY010000000
VERSION     DAJVTY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.180"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            168..275
                     /locus_tag="OQQ66_003578"
     CDS             168..275
                     /locus_tag="OQQ66_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906354.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAIIWG"
     gene            complement(328..1086)
                     /locus_tag="OQQ66_003579"
     CDS             complement(328..1086)
                     /locus_tag="OQQ66_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1906355.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1373..2302
                     /gene="pfkB"
                     /locus_tag="OQQ66_003580"
     CDS             1373..2302
                     /gene="pfkB"
                     /locus_tag="OQQ66_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1906356.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2403..2693
                     /gene="ghoS"
                     /locus_tag="OQQ66_003581"
     CDS             2403..2693
                     /gene="ghoS"
                     /locus_tag="OQQ66_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1906357.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2799..3659
                     /locus_tag="OQQ66_003582"
     CDS             2799..3659
                     /locus_tag="OQQ66_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1906358.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3700..4236)
                     /locus_tag="OQQ66_003583"
     CDS             complement(3700..4236)
                     /locus_tag="OQQ66_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1906359.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4383..5051
                     /gene="hxpB"
                     /locus_tag="OQQ66_003584"
     CDS             4383..5051
                     /gene="hxpB"
                     /locus_tag="OQQ66_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1906360.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5214..5804
                     /locus_tag="OQQ66_003585"
     CDS             5214..5804
                     /locus_tag="OQQ66_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1906361.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            5937..>7327
                     /locus_tag="OQQ66_003586"
     CDS             5937..>7327
                     /locus_tag="OQQ66_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCS1906362.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
BASE COUNT         1842 a         1787 c         1812 g         1886 t
ORIGIN      
        1 tattttcgat atatatgttg atgcataata ttattttttc ataaaacgca gaaacagtga
       61 ccaggtgtac aacgccaccc aacggctgta ataacaaaaa atcccttgat gcccgtccct
      121 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
      181 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
      241 tgttgctacg ttgggcagca attatttggg gctgagcagt gacaaacgaa gacagccaga
      301 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
      361 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtaa cgttgtaagt
      421 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
      481 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
      541 ccatgcataa gccccggagg catagcccag cggctgagtt tcactggcat tgtcggtata
      601 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
      661 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
      721 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
      781 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
      841 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
      901 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
      961 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1021 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     1081 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     1141 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     1201 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     1261 tatatatatt tatctgcaaa attttaaata aagctccaat aaatcatatt gttaatttct
     1321 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     1381 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     1441 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     1501 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     1561 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     1621 agaagccaaa gactggaccc ggcagaattt acatgtacat gtggaagcaa gcggtgagca
     1681 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     1741 gcaagttctg gaaattgaat ccggggccat cctagtcata agcggaagcc tgccgccagg
     1801 tgtgaagctg gaaaaattaa cccaactgat ttccgcttcg caaaaacaag ggatccgctg
     1861 catcgtcgac agttctggcg aagcgttaag tgcagcactg gcaattggta acatcgagtt
     1921 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccgga
     1981 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     2041 ttcccttggt ccacaaggag cgctgggtgt tgatagtgaa aactgcattc aggtggtgcc
     2101 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     2161 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     2221 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     2281 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     2341 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     2401 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     2461 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     2521 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     2581 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     2641 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     2701 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     2761 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     2821 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     2881 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     2941 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3001 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     3061 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     3121 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     3181 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     3241 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     3301 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     3361 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     3421 ttttcgaccc tgcctgctac tggggtgacc gagagtgcga cctggcgatg ttaccgctgc
     3481 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     3541 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaaggttat
     3601 ttggcggtca gcatttggtt attgctcagc agtcactgga tagattatta gcagcatgat
     3661 atgggttgaa gataatggcc gctctgtgcg gccttttgat taaataaacc caagcagaga
     3721 aaagaacaca taacctgcag caatacaaat aaccggcagg atatacagtg agaaaaactg
     3781 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     3841 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     3901 cgcctggcga ctcattcggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     3961 gaaaatcacc cagaacataa cgttcacgcc accattaaaa ttgggcgtcg gggagttata
     4021 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagtaaa
     4081 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     4141 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     4201 caaaacagca atgtgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     4261 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     4321 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     4381 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     4441 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtggata
     4501 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     4561 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     4621 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     4681 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     4741 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     4801 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     4861 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     4921 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     4981 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     5041 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     5101 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     5161 gaactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     5221 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     5281 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     5341 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     5401 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     5461 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     5521 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     5581 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatccta gttccccaaa
     5641 agggcaacca actggaacgt tttatctgta tggcattatt tgtctggtcg gtatggatgc
     5701 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaataatc aataccttgc
     5761 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaagac aaaaatcacc
     5821 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     5881 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     5941 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6001 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     6061 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     6121 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     6181 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     6241 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     6301 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     6361 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga
     6421 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     6481 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     6541 tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt cttagccgct atcgataccc
     6601 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     6661 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     6721 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgcagtgcat ggcattctgc
     6781 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     6841 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     6901 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     6961 gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg
     7021 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     7081 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     7141 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     7201 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     7261 agtggctgaa gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac
     7321 accgtta
//
LOCUS       DAJVTY010000181         7320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVTY010000181 DAJVTY010000000
VERSION     DAJVTY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.181"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..213)
                     /locus_tag="OQQ66_003587"
     CDS             complement(<1..213)
                     /locus_tag="OQQ66_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906363.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLR"
     gene            complement(903..2612)
                     /locus_tag="OQQ66_003588"
     CDS             complement(903..2612)
                     /locus_tag="OQQ66_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1906364.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3044..3763)
                     /locus_tag="OQQ66_003589"
     CDS             complement(3044..3763)
                     /locus_tag="OQQ66_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1906365.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3888..4451)
                     /locus_tag="OQQ66_003590"
                     /pseudo
     CDS             complement(3888..4451)
                     /locus_tag="OQQ66_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4435..4693
                     /locus_tag="OQQ66_003591"
                     /pseudo
     CDS             4435..4693
                     /locus_tag="OQQ66_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4802..5380)
                     /locus_tag="OQQ66_003592"
     CDS             complement(4802..5380)
                     /locus_tag="OQQ66_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906366.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5801..6038)
                     /locus_tag="OQQ66_003593"
                     /pseudo
     CDS             complement(5801..6038)
                     /locus_tag="OQQ66_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6225..6654)
                     /locus_tag="OQQ66_003594"
                     /pseudo
     CDS             complement(6225..6654)
                     /locus_tag="OQQ66_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6702..6887)
                     /locus_tag="OQQ66_003595"
     CDS             complement(6702..6887)
                     /locus_tag="OQQ66_003595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906367.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEITRCTEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            7050..>7320
                     /locus_tag="OQQ66_003596"
     CDS             7050..>7320
                     /locus_tag="OQQ66_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1906368.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKR"
BASE COUNT         1979 a         1706 c         1370 g         2265 t
ORIGIN      
        1 acgcagttgc ataacttcag attccagctc agccactgtg cgggagcctg gtgtattgag
       61 cccttttctt gcggcggtaa cccattgtcc taaagtgcct tcaggaacag ataatcggga
      121 agcgccttca ctgatcgaaa gctgattttc aggaaccgtt ttgacggctt cggctttaaa
      181 ctccgtagag taacgttggg tttttctgct cattattggc tccttctgat gccattctat
      241 ttcaggaagg agtgtccgtt aaactcaggc tacctcagat aagcttgata caactggtct
      301 ggtatataca gtttctttga gtgcggttac gtccacagag gtaacggtaa aagtaacccc
      361 ttgtcgggac cactccgacc gcgcacagag gcattttcat cgctgacagg ccgcattgcc
      421 tcgcagggct ggaggaggat cggtgatatc cggacgaccg taccggctta cgccgggctt
      481 cggcggtttt actcctctgc catgtgcaac aacggagtgc cgccttacat gccgctggcg
      541 cggcatatcc tggtaacatt tttgttgata ccaccttccg gccttatcct gaccagtggg
      601 ctttcctggc atcgatgata ccgatgaacg cgcatgatat tgagccgacg atattacggg
      661 caactggaaa tactcaccct ctggatgtga cctttatcca tgaagaagat ttggccatac
      721 catggaaacc tgaacaatca tcagtttacg cccatgtaaa cccctacggg atatttgaac
      781 tcgatatgga aacaaggttg ccaatcgagg tggttgcctg aaagttggca gttacccggt
      841 cactacacct tgttggagtg aacatatcaa aaacaggtag ttacacaatt taaattacag
      901 tctcataaaa aatgattaaa tctatttatt atttcaataa acgttttttt catcttcttt
      961 gaaaattgtt ctgctatagt atccggttcc agattgttct cttttattat tctacaatac
     1021 tctaatattg tttctttgtc tctatttact acagatacaa atacaccagg aataccatct
     1081 ttattagttg cggacagcat ttcgaccaat cgtcttgtgg taaaactata gttttttaga
     1141 atattacaaa aggcacgtat tgtttcggta tggccattat aaaatgccat aaaaagtcct
     1201 gatgtaccgt tgggattttt tatccataat aattcatcta tatattcctg gtgagggggg
     1261 agtattttta gtatatctcc atacgccata atggcctttg tgtgcccatt ttgaagtgcc
     1321 ataaacaacc catgctttac tttcaataat tctttaattt gttcactact aagcattttc
     1381 ttattaagca gacttccgaa catacgaata ctatctgcat gtccattctg caatgcaaaa
     1441 aataacccag gtaaatcatg gctgtctttc gcagctaata gatataatct cttatcggaa
     1501 gttaacgggg ttttaaagac tatttcccca taggctttta ttgtgtctac atgtccgttc
     1561 ataaaagaca tgcataaacc cggcacattg tttgccccta ccgcagcaag caatgtaagt
     1621 attgtttctt gactaagatt gcacatattt aatatcttac catatacttg aataatgtct
     1681 gaacatccgt tttgaagtgc tatataaaat gcaggagtac catcagagct tctcgtggcc
     1741 agaaggttca ccagtttaga ttgagaaatt tcaccatttt tgtatttttc catcagtata
     1801 aataatactt ctgtcagatt ctttgttatc gcatgatata taactctgga attgatcaat
     1861 tcatctggta aaagtttcac tgtgtttgtt agaggggaat tatgcctatc ttcaaatatt
     1921 acctgcccgg gttgctccag accataaatt ttccaataaa aatcatcaaa aaaatcctta
     1981 gcggataatt gactcagttc gaaatggcta tcacaagtaa atacagtacg tttatgagta
     2041 acagttcgat taggatcgaa aaactgaaca acccattttt cactttcttg tacgcgcttt
     2101 attttcaacc ctaacgccat ttggtggttt aatgtgcatg ctgataatgt tctgacaaag
     2161 tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa ctgttcccca ctttttattt
     2221 tccacaaaat aaatataagc tggtgatttt gaaaaatctt ccgttttttc caaacataca
     2281 ttttttgcaa tagcatcagg ggtgctaaga agctgatagt ttaccttccc atttgtttcc
     2341 ataaaattca tttcccaata ggaagctagg tgacggcaaa ctatttgtct tccatatata
     2401 tcttttgcca ctccatttat atttaccttc acatttctct tttctgaaaa ataaggtgct
     2461 gttttattct taaactgaat tagttcactg gagtttgaga ccctcataat agaacttgtt
     2521 attgttctat tatctctgtt aacagacata tctgttgata acgaattttt tgttgaaaat
     2581 atactagatg aaaatgtttt atttaacggc atagaagaca accatagaga tgttataata
     2641 ctattagatt gcagttacta atagtaaaag cctccttaca aggaggtagc ttattcagaa
     2701 tgctcctata agatagcgag ctcgtcaacg atacagatga tctgattata ttgtgcaaaa
     2761 ggagaacagg aaaataggca ctggaaaaga tgaaggcctg atgggagcat taactaatgc
     2821 aaaatccgac aggttaatat cccgaaaagc aatcaaacga ctcaaacagg agatgcacgc
     2881 tgaagtggtg gacaactgtt cggatttcag atgagtagct cagcagttta tgagccgaat
     2941 gctctgtcac ggtcaggaat caggctgcat acgctgtgcc aaatctaatc gtagttattc
     3001 taaccagttt tccagctaca aggtggtgta gctggcatct tctctactct atcatcaaac
     3061 gataaaatgg ttcctctcgt aacatttgct cttgcctttc acttccatct cgatcgcttc
     3121 gtagttcatt acgatagctg acatatttcc agtcttcagg tctaacatct gacgccggat
     3181 actccccggg agcgattttg cttcttaata gattagactc gagacacatt ataaattgtc
     3241 gtattttatg aagtaataaa gcactatatt gcgagcattc ctgatctgat tttacatata
     3301 gcgtaaactg agctcctatc cccacacgag attgctgaga aactcgattc atatcagtta
     3361 tcttccattt atctatagga ctgtcctctg aaaataataa accagacaaa atttggaatg
     3421 ccagaggaac ttgctcccta gctatactaa tatgaaactt gtccccaaca aaatccccct
     3481 tactctgagg attttcacga catgcatgaa taaacacatc tccactatgg ttcacaatct
     3541 ggaatccttg gtacatgaca gaaaaaaatt gacatatatc agagtaagtt ggcacgttcg
     3601 atgctacttt gaaatagtac gctgggtatt gccgtatttg gttgtacaat atattgaagt
     3661 tatttttcaa tctttcattc gcagaaagca tctgcggtat ttcagacctc acaacattca
     3721 aagaatctaa atttagtttt agacagggct tttttatggg catagaaaac gtcctctata
     3781 aaatagatat attatcttat attgattgtt tatcatcttt tataaaacga tacattatat
     3841 gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg ggaaggctta gaacgccata
     3901 ttcagtttcc catagtggaa catcactccc agcttaaagc gctcccggtt gcggtagccc
     3961 ttggctttta ttctcagcag cctgatcttg ctgttcagtg cttccgcatt tccgtttgat
     4021 acgccatggc gcatggcgtt caggataccg tacaaccttt tccctatcgt tttagccaca
     4081 ttcttcatca tggggacgtc actgttggcc gccagagaca gccatctctg ccagtcgttg
     4141 cgtctttcct cgctccatgg tctgctccag atatcctttg ccagttcttt cagcgcccag
     4201 cacaggctcg tcagcttcat ctgcgcacgc agccatatca gcttttcctg cctggattcg
     4261 gtcatccatt tatcgctgtg ctgccacagg aagcgagttc cctttgcctg acgacgactt
     4321 tcaacaggaa gatgcggatg ttcattctgg cgggttttat ccaccacctc acccggttgt
     4381 ttcgccacat gaaagcgatc gaaggctatt ttctcaaccg cattgggtaa gtggatgcgc
     4441 gctgctctta gtttgctgag ccagtttagc attgccttct acatgaccga tgcctgagcc
     4501 ggtataccga actttattgt cctcaaccag tcatgttata agcaagaaat gcactcagcg
     4561 gatagagcga cataatctga acctgcggac acatcttaag cggcttaccc gcaaaaccat
     4621 atgcttctca aagtctgatg atatgcacta taagatcatc ggctggtatc tgacaatcaa
     4681 ccaccaccac taaatctgcg tcacgacctg caactgagtc tggttgaaca ggctgaggcc
     4741 ctgctgaagc aacgcgaacc tctcgactct tgttggattt taccaaatag tttttctaaa
     4801 attaaacaca tttatataag ttctttgctg aacaaaacgc agcccgccat cctttttcat
     4861 tcataaattc agcaatggca gataaaactt tttcatcaag catataaaaa ataatgcaag
     4921 tagactcaat atgcctcata caaataaggc cgcacatatc cgtaatatat ttgtcaatgc
     4981 tgctataatt tgcatggtaa tatgccatgc tattatctga tgattttaca tatgcagtat
     5041 gacatatttt tccttcacga ttaacaaaac tgacaaaacc actttcacca ggagtaatat
     5101 ataaagatga atttaacgtg cgctcactaa tattcattaa ttgttgaaat tcaatggaat
     5161 agttttcagt taatgtatct attatcttgg ctttgtcttt ctttgttatc tcattgtctt
     5221 tttcggcaag aaaaattgcc actttatgac atgcattcag ctcgttaatt ttacctgata
     5281 aaactgcttt tcttaccaca tcgttgaact catcgatagt gtgtttttta ttatatatat
     5341 catgaaaaat atagcctaaa gacttaaacg agactttcat tttatattct ccttaaatat
     5401 taacaggata tatatatcac attttcctct atattttcag aaaccagcta tcattttccc
     5461 cattactggt acgtcgcttc agatatttcc gcagcaagcg ataaccctgc gcactatgag
     5521 gatgccattc attataatgt ttgaacacct tcgcgagact actttactgc tgttaacacg
     5581 tcaggttcga acataacact gatttaatca tatttcatcg ttttgacgaa gttttctgcc
     5641 attccatttt gcaccgccgt gtgtttgggg gcaaggccga ccatctgtgt ttcattcgcc
     5701 agatataagt gatgagatgg cattctccct ccttcacggc agaagcattt tgaccgttta
     5761 ggggatgcgg acgattttca tgcctgctaa gatactttcg ttactgatgc tgtccgtttt
     5821 ccaccatttc atcatcactg attggcgttg tgtttttggt cagcactcca gctttacgtt
     5881 tctcttttaa ccggtaactc tctcctttga tattcagcgt gtagcagccg atctaggatc
     5941 gctgttgcca gcacgtgatc tccgaacatt tctccccagt ctgcgacccc tttgtttgac
     6001 gtcagtatga tgctcgcttt ttcatatcga cagttcatcg ataggaattt aaatccccaa
     6061 aagattaaaa aagcaccgaa gaatgcgcgt ttattcaaca caacctgatc atcaagtaat
     6121 cggtatagac acttaatatt gcttacattg attgattcaa tgtatctctg atggtttttt
     6181 gctccgttcc cctcaacatg gacgcaactt cccctctgcg actctcagcc gcaccaccgt
     6241 attcgagcca gcggctcatg catcataacc tgctctgcca gacggtaacc cctcttcagc
     6301 cccgtaaacc gcatctgacc tccgcacagc tcgcacttca gcgggtcaac cttcagtcac
     6361 tgctgataca tccctctcct tgtgatttgc ctcgccgttt ttctcactgt ctccgttatg
     6421 gcgtacacca catcttccag taatcgccgc ttcaccggac tcaggacccc gtatcaccat
     6481 ccggaacccc ttatccgcca catgccagga gaacctttcc ataaactcgt cgccactcat
     6541 cacaaggttt tcttctcgtt ttgtccggtg gctgttgtaa cgcaggccaa tttcatcctg
     6601 accggcataa tgttccaggc ggctcatctg caccggctgc ttctccagcc agttaagcca
     6661 tcggtttccc tgctgaaaaa tatcggcaaa acggggaagc atcagaaagg cggaataacg
     6721 ccatccggcc agtgaaccgt accgcattca gggcaataca tgccgtcggc gttgataccg
     6781 gcaggaattg tcgcaaattc ccgctccgtg cagcgggtaa tttccggata cccttcgtca
     6841 tcaacacgta caaaccagaa gaccagtttt ttgtttccgg catccacaaa gaacggaata
     6901 ttcaggtctg cgcaacactc aacggcatcg tcaaaattat caaagcgcag aacctcagct
     6961 tcttcgtaac cggctcattt aaaccgtctg gtctgtttcc tccggctcca caaaaataat
     7021 gtccatcatt ttgaagtgga cactatcgta tggaataccg gacctggatc actgaagctt
     7081 tacgccttca tttcgaagaa catttaccac gggttgtggc cggacgtcgc ctgggcgtac
     7141 caaaatcaac tgtctgtggt atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc
     7201 tgcccgcagg tatgtcggag caggaacttg atgcccttct ttacggaagt gcctccacag
     7261 tacctgtcgt acttactgaa agcaccgtta tgcccaaact cccggtggta aaaaaacgtc
//
LOCUS       DAJVTY010000182         7052 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVTY010000182 DAJVTY010000000
VERSION     DAJVTY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.182"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1473
                     /locus_tag="OQQ66_003597"
     CDS             <1..1473
                     /locus_tag="OQQ66_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906369.1"
                     /translation="VVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEGKT
                     CRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDAFH
                     ARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKLDH
                     LRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSFQA
                     GALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALREY
                     LRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSLPA
                     SACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAETAP
                     VAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAVGF
                     YRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            1598..2059
                     /locus_tag="OQQ66_003598"
     CDS             1598..2059
                     /locus_tag="OQQ66_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1906370.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2100..2570)
                     /locus_tag="OQQ66_003599"
     CDS             complement(2100..2570)
                     /locus_tag="OQQ66_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1906371.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(2617..3336)
                     /gene="yehT"
                     /locus_tag="OQQ66_003600"
     CDS             complement(2617..3336)
                     /gene="yehT"
                     /locus_tag="OQQ66_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1906372.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(3333..5018)
                     /gene="btsS"
                     /locus_tag="OQQ66_003601"
     CDS             complement(3333..5018)
                     /gene="btsS"
                     /locus_tag="OQQ66_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1906373.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            5240..5305
                     /locus_tag="OQQ66_003602"
                     /pseudo
     CDS             5240..5305
                     /locus_tag="OQQ66_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            5533..5781
                     /locus_tag="OQQ66_003603"
     CDS             5533..5781
                     /locus_tag="OQQ66_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1906374.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(5897..6166)
                     /locus_tag="OQQ66_003604"
     CDS             complement(5897..6166)
                     /locus_tag="OQQ66_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1906375.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
     gene            6223..6891
                     /locus_tag="OQQ66_003605"
     CDS             6223..6891
                     /locus_tag="OQQ66_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1906376.1"
                     /translation="MVKLMKKNTDDGAKIYTPITLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFETYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT         1864 a         1754 c         1652 g         1782 t
ORIGIN      
        1 gtcgtactgg cagtacaggc ctttgtccag gcaaaagcgc agcaagcgga attgactcat
       61 ttaatctggc agatgcgcag cgaacacgtc acatcatctg acgatccgtt tgccagcgaa
      121 gaaggcaaaa cgtgtcgtca atatgttcaa caactaagcc aggcattatg gctgagcggc
      181 atcagccagc cgcttattca ctacgaggcc gctttcagcc gcgcgcagca agcagcggaa
      241 cgctgcagct ggcgatgggt gagtgaatcg ctacggcaac tgcgcgccag cgttgatgcc
      301 ttccacgccc gcgccagcca ctatcatgcc ggagaatgct tacgtcagct tgcggcatta
      361 aacagtcgat taaactgcgc acaagagatg gcccggcgcg acagtgttgg tgaagttcct
      421 cctgtgccgt ggcgcacggt cgttggctct ggcattgccg gagaagcaaa gcttgatcat
      481 ctgcggctgg tgtctttagg tatgcgttgc tggcaggata ttgagcatta tggtttacgc
      541 atctggttta ccgatcccga caccggcagt attttgcatc tttcgcgtag ttggccgcga
      601 agtgaacagg aaaactcacc ggcagctacg cgtcggctgt ttagttttca ggctggcgca
      661 ctggcgggtg ggcaaattgt ttcacaagca gcaaaacgca gtgccgatgg cgagctgctg
      721 ttagctaccc gcaaccgctt aagcagcgtt gtgccgctgt cgccagacgc ctggcaaatg
      781 ttgagcgcgc cgttacgcca gccgggcatt gtggctttgc gggaatattt acgccagcgt
      841 ccccccgcct gcatacggcc tcttaatcag gtggataact tatttattct gccggtcgct
      901 gagtgtattt cactcggttg ggacagcggc cgccagacgc tggatgcgca ggtaatcagc
      961 ggcgaaggtg aagataatct gctgacgtta tcccttccgg catctgcctg ttctcctttt
     1021 gccgttgaac gcatggcggc gcttttgcaa caaacagacg accccgtgag tctggtttct
     1081 ggctttgtca gctttgtaga ggggcaattg acactggaac cacgggtgat gatgacaaaa
     1141 acccgtgcct gggcgctgga cgcggaaact gcgcctgtgg caccgctacc ttctgccagc
     1201 gttttgcctg tgccgtctac cgctcatcag ttgctgatgc gctgccaggc gttacttatt
     1261 caactgctcc ataacggctg gcgctatcag gaacagagcg ctattagtca ggcagagttg
     1321 ctggcgaatg acctcaccgc ggtgggtttt tatcggctgg cacatgtgtt ggtacaattt
     1381 cgtaatacag aaagcgaggc acgggtagaa gcaatgaata acggtgtttt gctttgcgaa
     1441 caattattcc ccatgcttca gcaacaagga tgaaatagtg ctttttacta agagttctac
     1501 tccagttccg gactgctcac gccacggtat taggcatatc ctatatagcc cctggtgaga
     1561 gtcaccagtt tcttgattaa ataaaatgga gttttacatg aaggctttca ataagctgtt
     1621 ttccctcgtt gttgcatctg ttctggtttt ctctcttgct ggctgcggtg acaaagaaga
     1681 atcgaagaaa ttcagcgcca atctgaacgg cactgaaatt gccattacct atgtctacaa
     1741 aggtgacaag gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattagtgc
     1801 aaccaccaaa gaagatgctg ccaagacact tgagccgtta agcgccaaat acaaaaacat
     1861 cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa acgtgactat
     1921 cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca acgtttctgc
     1981 tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg
     2041 ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg tatggccgcc atacccgtct
     2101 tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga
     2161 tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt aatttccggc
     2221 atcgaaacgc ggaactgctg ttcggtgagg atagccagaa tgtcatcggt tttcagcgaa
     2281 aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt
     2341 gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgacgac
     2401 aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg
     2461 atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag gtcattatta
     2521 ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat gaggccttca
     2581 ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa
     2641 gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc
     2701 ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca gatactgacg
     2761 atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt cggtaaagcc
     2821 ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga caaatgccac
     2881 atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa atttcagcgc
     2941 ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac gcaatcgcgc
     3001 cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa aggcatgttc
     3061 ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat acgggcgatg
     3121 ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag
     3181 aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg cgtttgaaca
     3241 ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acacgcaggt tctcccgtgc
     3301 taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagttgt
     3361 aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata gtcatcgcca
     3421 aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc
     3481 ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg
     3541 gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt
     3601 tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata
     3661 gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc
     3721 acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa gtttttgcgg
     3781 aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg gcggatcacc
     3841 gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc gtgaagcagt
     3901 ttgatttctg actgggtgag catcgctttt tgccgctcat attgcccggc aagaatctgc
     3961 gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact gaataaacgg
     4021 tttttggctt catacaattt gatggtgccc atcacccgct gattttcacc acgcaacgga
     4081 attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg
     4141 ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta agtcgaagaa
     4201 atcggtttgc cgggtaaatg gtggtcgtca ccaattccag taaaggccag caatttctct
     4261 cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg tgccactttc
     4321 atgctgttca cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc agccactttc
     4381 agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat
     4441 atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggtgc agcaatatta
     4501 ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat caacatttgc
     4561 accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagta
     4621 cgcccgcgac gaatcaggat gctgtgtacc aggccgccga gcaatccttc aacgatggtc
     4681 gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa cccgccggtc
     4741 aggccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcgcctatc
     4801 gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag
     4861 aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt gacctgcatt
     4921 aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc
     4981 tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca cacttccctt
     5041 taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc agaaaaatga
     5101 gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatagatgc
     5161 acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa accgggacct
     5221 cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt tgctttgtga
     5281 tattaaccct gtcacgttac gcgcgtgacg caacatttta aaaaatcgac gaagatcgtc
     5341 caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg acgaacttcg
     5401 acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca taaacaatat
     5461 cagaaattga tactggttat ccatacagat aaaaataaca cctgtacata actacagtta
     5521 caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac taaaatgcca
     5581 aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa acgttatgac
     5641 gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac acgcaccgca
     5701 gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg ggagtctgct
     5761 gacgagtggt ttgttcacta ataaacacgt aaaatcggta acggctggaa atcattcaat
     5821 actcgcacta tcgaaagttt accagccaac cgcagcacgt cctgcatacg tcgtgtctgc
     5881 ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata tcaggtgcag
     5941 ttgatgtatc aacacagttc agcaacaccc gatacttctt ccaggcttcc agcaacgagt
     6001 tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca atatgctcac
     6061 tgaattcctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgctgta
     6121 tcgaagcata ccaggctatt tcaatatcgc tatgctgcag cagcatttaa ccccttgtaa
     6181 ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa attaatgaaa
     6241 aaaaacacag atgatggggc taaaatttac acaccaatta ccctaaagct ttatgactgg
     6301 tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt
     6361 ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt
     6421 taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc
     6481 ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat
     6541 gtttttgaaa cttatcccgc ggcgttacat ggtcaatttg attccatttc catgttttac
     6601 cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg
     6661 caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg
     6721 cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac
     6781 acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa
     6841 accaaggttc aaggtactgt agtaatgttt tctgcttcag ggaaaaaata gcatccaacc
     6901 gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg ttgcgacttt
     6961 acctctattg ataatgcatt ccggcagaac attcaaatat gaatgtacga tatttaacta
     7021 accgaaaaac aaaatatagc acaggcgaga ca
//
LOCUS       DAJVTY010000183         7029 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVTY010000183 DAJVTY010000000
VERSION     DAJVTY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.183"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            86..172
                     /locus_tag="OQQ66_003606"
                     /pseudo
     CDS             86..172
                     /locus_tag="OQQ66_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            473..562
                     /locus_tag="OQQ66_003607"
                     /pseudo
     CDS             473..562
                     /locus_tag="OQQ66_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            586..5100
                     /locus_tag="OQQ66_003608"
                     /pseudo
     CDS             586..5100
                     /locus_tag="OQQ66_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5101..6750
                     /locus_tag="OQQ66_003609"
     CDS             5101..6750
                     /locus_tag="OQQ66_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1906377.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6755..>7029
                     /locus_tag="OQQ66_003610"
     CDS             6755..>7029
                     /locus_tag="OQQ66_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906378.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGAS"
BASE COUNT         1695 a         1744 c         1969 g         1621 t
ORIGIN      
        1 ttgatcctca ttgtttatat attaatatgt tcgtgcaaat ggagtgagga ttctttaaaa
       61 aaacgctcct gtaaatagtt aagaacagga agaactgttg gggatgtggc tggccgaaaa
      121 tgaaggggtg aagttctggc tcaatgtgct gactgaactg aaaaaacgcg gttggtcgcc
      181 ctaccttttc atcaagccag tagtctgccc accactgcat catttctctg cgcttatcga
      241 gatactgagc atggttgtaa attcaactaa cccagtgcgt tgacaaaaaa ttagcgcaag
      301 aagacaaaaa atcaccttgc gctaatgctc tgtatcaggt cactaatacc agctaagtag
      361 ttgattcata gtgactgtat atgttgtgct ttgtcgcatt ttgtagtcta ttatttaatc
      421 tgtaacaacg ataatacaat gaattttaat tggtttttgt gattttgtcc ttcttctgac
      481 atggaaggac acccgatgga tacccaacga ttccaatccc aatttcattg gcatttatcg
      541 tttaaatttt ctggcgcgat agcagcgtgc ttgtctctgt ctcttgtggg aacagggctt
      601 gctaatgctg atgattcgct tccttccagt aactatgcgc cgcccgccgg gggaacattc
      661 tttttgcttg ctgacagcag ttttagcagc agtgaagagg cgaaagtgcg actggaagcg
      721 ccggggcgtg attatcggcg ctatcagatg gaagagtacg gcggcgtgga cgttcgcctg
      781 tatcgtattc ctgacccgat ggcatttttg cgccagcaga aaaacctgca tcgcattgtg
      841 gtgcaaccgc aatatctggg cgacgggctg aacaatacgc tgacctggct gtgggataac
      901 tggtacggca aatctcgccg cgtaatgcag cgtactttct cttctcagtc acggcagaat
      961 gtgactcagg cattacccga attacatctc ggcaatgcca ttattaaacc ttcccgttat
     1021 gtacagaaca accagttttc cccgctgaaa aaatatccct tggtggaaca gttccgttat
     1081 ccactatggc aggctaaacc ggtcgagccg cagcaagggg taaaactgga aggcgcatcc
     1141 agcaatttca tctcgccgca gccgggtaac atttatattc ctctcggcca acaagagccg
     1201 ggactgtacc tcgtcgaggc gatggttggt gggtatcggg cgacgacggt ggtgtttgtt
     1261 tccgataccg tggctcttag caaagtgtca ggcaacgagc ttctggtgtg gaccgcgggt
     1321 aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt ggactgacgg tcttggcgtg
     1381 atgacccgcg gtgtgaccga tgacagcggt accttgcagt tacaacatat atcgccagaa
     1441 cgttcataca ttctgggtaa ggatgctgaa ggcggcgttt ttgtctccga gaacttcttc
     1501 tacgaaagcg aaatctacaa cacccgcttg tatattttta ccgatcgccc gctatatcgc
     1561 gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt tccacgatcc gttgcattca
     1621 tcccccatcg tcagcgcccc ggcgaagctt tcggtgctgg acgctaacgg cagtctgttg
     1681 caaaccgtcg atgtcacgct ggatgcgcgc aatggcgggc agggaagttt ccgcctgcca
     1741 gaaaatgccg ttgccggagg ttatgagtta cgtcttgctt accgcaatca ggtctatagc
     1801 agcagttttc gcgtggcaaa ctacatcaag ccacatttcg agattggttt agctctcgac
     1861 aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac tgcaactgct ctacccggat
     1921 ggcgagccgg taaaaaatgc ccgcgtgcag ttaagtttgc gcgctcagca attatcaatg
     1981 gtcggtaacg atttgcgtta tgccggacgt ttccccgtgt cgctggaagg cagcgaaacg
     2041 gtgtccgacg ccagcggtca tgtggcgtta aatctccccg ccgccgataa accgagccgt
     2101 tatttgttaa ccgtctccgc cagtgacggc gcggcgtatc gtgtcaccac caccaaagag
     2161 atcctcattg aacgcggtct ggcgcattac tcattaagta ctgccgcaca atacagtaat
     2221 agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat cttcaaaaca ggttcctgtt
     2281 acgtatgaat ggttgcgtct cgaagaccgc acgagccata gcggagagct accgtcaggc
     2341 ggcaaatcct ttaccgtcaa tttcgctaaa cctggcaact acaatctgac attacgcgat
     2401 aaagacggct taattctcgc tgggttaagt tatgccgtca gcggtaaggg cagcacggcg
     2461 catacgggta cggtagatat cgtggcggat aaaacgctgt accagccagg cgaaaccgcg
     2521 aagatgctga ttacctttcc ggagccaatt gatgaagcat tattgacgct ggaacgcgat
     2581 cgcgtggaac agcagtcgct gctttcgcat ccggcaaact ggctaacgct acaacgttta
     2641 aacgataccc agtatgaagc ccgggttcca gtgagcaatt cctttgcgcc taacatcact
     2701 ttttcggtgc tgtatacccg taatggtcag tacagttttc agaacgccgg gatcaaagtt
     2761 gccgttcctc agcttgatat ccgggtgaaa acggacaaaa cccattacca gccaggtgaa
     2821 ctggtcaatg tcgaattaac ctcgtcgctg aaaggtaaac ctgtttctgc gcagctaacg
     2881 gtaggcgtgg tcgatgaaat gatctacgcg ctgcaaccag aaatcgcgcc gaatatcggc
     2941 aaatttttct atccgctggg gcgtaacaat gtgcgtacca gctccagtct gtcgtttatc
     3001 agctacgacc aggcgctctc cagcgagccg gttgcgcctg gcgcaactaa ccgcagcgag
     3061 cggcgagtaa aaatgcttga acgtccacgg cgtgaagagg tggataccgc ggcatggatg
     3121 ccgtcactca caaccgataa acaaggcaaa gcgtatttca cgttcctgat gcctgattcg
     3181 ttaacccgct ggcgtatcac cgcgcgtggg atgaacggcg acgggctggt cgggcagggg
     3241 cgtgcttatc tgcgttcgga aaaaaatctc tacatgaagt ggagtatgcc aacggtgtat
     3301 cgcgtgggcg acaaaccggc ggcaggactg tttatcttca gtcagcagga taacgaaccg
     3361 gtggcgctgg tgactaaatt tgcaggcgct gagatgcgcc agacgctgac gctgcacaaa
     3421 ggggcgaatt atatttcgct ggcgcagaat attcagcaat ctggcttgtt aagtgcagaa
     3481 ctgcaacaaa atgggcaagt gcaggacagc attagcacaa aactgtcttt tgtggacaac
     3541 agcaggcccg ttgaacagca gaaaaatgtc atgctcggcg gtggcgagaa cgcgctgatg
     3601 ttgcccgagc aggcgagtaa tatccggcta caaagtagtg aaacgccgca ggagattttc
     3661 cgcaacaatc ttgatgcgtt agtcgatgaa ccgtggggtg gggtgatcaa caccggtagc
     3721 cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg atcatcaaag tgccgccgct
     3781 aacgacattc gtcagatgat tcaggataac cgtctgcggc tgatgcaact ggcggggccc
     3841 ggagcgcgct ttacctggtg gggtgaagat ggcaatggtg acgccttcct tacggcatgg
     3901 gcatggtacg ccgactggca ggccagctag gcgctcggcg taacgcaaca accggaatac
     3961 tggcagcata tgctcgacag ttatgccgag caggcggata acatgccgtt attgcatcgg
     4021 gcgctggtgc tggcgtgggc acaggagatg aatctgccgt gcaaaacgtt gttgaaaggg
     4081 ttggatgaag ctatcgcccg gcgcggaact aaagatgaag atttctctga ggaagacatc
     4141 cgcgatatca atgacagcct gatcctcgat acaccggaat ctccactggc agatgcggtg
     4201 gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt tgaagtccac ggtgatgcca
     4261 caggttcagc aatatgcgtg ggataaagcg gcaaacagca atcagccgct ggcgcacacg
     4321 gttgtgctgc tcaatagcgg tggtgacgct acccagacgg ccgctatttt aagtggtttg
     4381 accgctgagc aatccactat tgagcgcgcg ctggccatga actggctggc gaaatatatg
     4441 gcgacaatgc ctccagttgt tttgcctgcg cctgcgggcg catgggctaa acataagtta
     4501 actggagggg gcgaatactg gcgttgggtt ggtcagggcg tgccggacat tctctctttt
     4561 ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc gtgagccggc aaaaatggct
     4621 caacaaagta acattccggt gaccgttgaa cgccagttgt atcggcttat ccctggtgaa
     4681 gaagagatga gctttattct gcaaccggtg accagcaatg agattgacag cgatgcgctg
     4741 tatctcgatg acatcacgct taccagcgag caggatgcag ttctgcgcta cggtcaggtg
     4801 gaagtaccgc tgccaccggg agccgacgtt gagcgcacaa catggggcat ttcggtcaat
     4861 aaacccaacg ccgcgaaaca gcaggggcaa ttgctggaaa aagcgcgaaa tgaaatgggc
     4921 gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa cggtcacttt ccgccatttg
     4981 ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg ctcgttatgt gcgttcctat
     5041 gcacctgcac agcaaagtgt tgcggcaggg agtgaatgga ccgggatgca ggtgaaataa
     5101 gtgaactggc gcagaatcgt ctggctattg gcgttggtta ctcagccaac gcttgcggag
     5161 gagacgccgt tacaactggc actgcgtggt gcgcagcacg atcaactgta tcagctgtcg
     5221 tcatcaggcg tgaccacagt ctcagcatta cctgactcgc tcaccacgcc gttagggagt
     5281 ttgtggaaac tgtatgttta cgcctggctg gaggataccc atcaaccgga gcagccctac
     5341 cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc aggctgggga gagcataacg
     5401 cgcgataccg cgctggtacg ttcctgtggg ctctattttg cgccgcagcg gttgcatatt
     5461 ggcgcagatg tgtgggggca atactggcaa cagcgtcagg cgcctgcgtg gttagcgtct
     5521 cttactacgc tgaagccaga aacctcggta acggttaaat cgctgcttga ttcattagcg
     5581 acgttacctg cgcaaaacaa agctcaggaa gtcttactgg atgtggtgct ggacgaggcg
     5641 aaaatcggtg tcgcatccat gctcggtagt cgggtacggg tgaaaacctg gagctggttt
     5701 gccgatgata aacaggaaat ccgtcagggt ggttttgccg gctggttaac cgatggtaca
     5761 ccgctgtggg ttactggcag tggcaccagc aaaacggtac taacccgtta tgcgacagtg
     5821 cttaatcggg ttttgccagt gccgacgcag gttgccagtg ggcaatgtgt agaggtggag
     5881 ctgtttgccc gctatccgct gaagaaaatc acagcggaaa agagtacgac ggcagtaaaa
     5941 cccggcgtac ttaatgggcg ctatcgtgtg acgtttacta atggtaacca cattacgttt
     6001 gtcagccacg gtgagacgac cttactgagc gaaaagggca aactgaaatt gcagtcgcat
     6061 ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg aggcgaaaag tacgccccct
     6121 gaggcagcga aagccatgac cgtggcgatt cacacttttt tacagcaaaa cgccaatcgg
     6181 gaaggcgatt gcctgaccat ccccgacagc agcgccacgc agcgtgtttc tgcttcgcca
     6241 gcgaccacgg gggcgcgaac gatgaccgcc tggacgcagg atctcatcta tgcaggcgat
     6301 ccggttcatt atcatggcag tcgagctacc gaagggacac tttcctggcg gcaagccacg
     6361 gcccaggcgg ggcagggaga gcgttacgac cagatactcg cgtttgctta tcccgacaac
     6421 agccttagcc gctggggagc gccacgctca acctgccagt tattacccaa agcgaaagcc
     6481 tggctggcga aaaaaatgcc gcagtggcgg cgtatattac aaggcgagac ggggtacaac
     6541 gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt tcccctatac cgatcggcag
     6601 cagaaacggc tgtttattcg taacttcttt acgcttcagg atcggctgga tttaacccat
     6661 gagtatctgc acctggcctt tgatggttat cccaccgggc ttgatgagaa ctatatcgaa
     6721 acgctgaccc gacaattatt aatggactaa tgctatgcga aagatttttc ttccgttgtt
     6781 actggtggca ctttcgcctg tcgctcacag tgaaggtgtg caggaagtcg agattgacgc
     6841 accgctctct ggctggcatc cggcagaggg cgaagatgcc agtttttcac aatcgatcaa
     6901 ttacccggcg tcgtccgtca acatggcaga tgatcaaaat atctcagcgc agatccgcgg
     6961 caaaataaag aattatgctg cggcgggtaa aattcagcag ggccggctgg tggtcaacgg
     7021 tgccagtat
//
LOCUS       DAJVTY010000184         7015 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVTY010000184 DAJVTY010000000
VERSION     DAJVTY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.184"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..248
                     /locus_tag="OQQ66_003611"
     CDS             <1..248
                     /locus_tag="OQQ66_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906379.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(217..1941)
                     /gene="aceK"
                     /locus_tag="OQQ66_003612"
     CDS             complement(217..1941)
                     /gene="aceK"
                     /locus_tag="OQQ66_003612"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCS1906380.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAALVEWLREILPGKTT
                     AELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVI
                     KDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQE
                     AAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIF
                     PGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSVSPGD
                     VFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFS
                     VRYGEMLF"
     gene            complement(2222..3519)
                     /gene="aceA"
                     /locus_tag="OQQ66_003613"
                     /pseudo
     CDS             complement(2222..3519)
                     /gene="aceA"
                     /locus_tag="OQQ66_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3549..5150)
                     /gene="aceB"
                     /locus_tag="OQQ66_003614"
     CDS             complement(3549..5150)
                     /gene="aceB"
                     /locus_tag="OQQ66_003614"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCS1906381.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5419..6348)
                     /gene="metA"
                     /locus_tag="OQQ66_003615"
     CDS             complement(5419..6348)
                     /gene="metA"
                     /locus_tag="OQQ66_003615"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1906382.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6505..6952
                     /locus_tag="OQQ66_003616"
                     /pseudo
     CDS             6505..6952
                     /locus_tag="OQQ66_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1683 a         1803 c         1820 g         1709 t
ORIGIN      
        1 accatgcacg cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag
       61 cgccacaaaa atactctgcc tttgagttgg cttttgaatt tggtcaccgg gttattgctg
      121 aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc
      181 gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg
      241 tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat
      301 gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa
      361 cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc
      421 gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg
      481 cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac
      541 cacacgcccg tgacgggtga caccgaagtt tttaaacagc atgtcgccag gaaaaatgtt
      601 agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg
      661 accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg
      721 gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa
      781 ttccattaat gccggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt
      841 gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg
      901 agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc
      961 aaagcccggc agcgtaaaca ccaacatcac cattccacga atacccggcg cttcaatgaa
     1021 ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg
     1081 cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca gaatttcccg
     1141 tagccactcg accagcgctg cataaaccat aaaataagaa cgcgcaaagc caaaaacaat
     1201 gctcgcttcg gcggtcgtcg tcaggcaggt atcaataaat aactcgccgt cgtccgtctg
     1261 gtggatcggc agtaagaagg gcaacgtgcc ggaaggcgtg atcagtttgc ccaccagcca
     1321 ggcggcttta ttgcggtaaa acagttcgtt cgccacctgt aaatgacttt ccgcgaggtt
     1381 gtctgtcccc agcgtttccg tcagatggcg aatgatgtaa tgaatgtcac ggcttttatt
     1441 ctgccagcgc aggcgcagcg gcaggtcgct gataacgcgc atcagtagag attcccagcc
     1501 gtgatcgggg tgaaagtctt tcgccagcgg gcggggaatg gtacgaaagc ggcgctctgg
     1561 ctgagagcta aagataaaaa gccgctcggg agtaagcgag cggtggtcaa ataaccgaca
     1621 gtacacggag ttaaaaaagc tctccgcaat ctcgaagcgc gggtaatccg gcaacagccg
     1681 cgtgtaatgc tctttaacgc gcagtaaaaa tgccgcgtcc gtactttggc cgttagtaat
     1741 gcagcgcagt tgctccacga ccagaccaac gtgatgatcg taaagatgga tacggttttt
     1801 catcgccagc tggacagcat gccagtcggc ctgttcgaaa cgctgctgcg ccccggaagt
     1861 cacttcgagg aatcgaccat actgagcgtc gaagccttgc aaaatggttt gagcaatcag
     1921 taattccagg ccacgcggca tttagcgccc tcatcaggag cagagaattg ccggatgcgg
     1981 cgtaaacgcc ttatccagcc tacgttcggc aacggttgta ggcatgataa gacgcgcaag
     2041 cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg taaatgcctt atccggccta
     2101 cgttcggcaa cggctgtagg catgataaga cgcgcaagcg tcgcatcagg catcgtgcac
     2161 cgattgccgg atgcggcgtg aacgccttat ccggcctaca gtcagcaacg gttgttgttg
     2221 cttagaactg cgattcttca gtggagccgg tcagcgcggt gactgaagac gtgccgccct
     2281 gaataatagt cgtcacttta tcgaagtaac ctgtacccac ttcctgctgg tgagatacga
     2341 aggtatagcc atctttcgcg gcggcaaatt ccggctgctg cactttctca acgtagtgct
     2401 tcataccctc gccctggtca taggcgtttg ccaggtcaaa catgttgaac cacatgctgt
     2461 ggatacctgc cagggtgatg aactggaact tgtagcccat atccgacagc tgctgctgga
     2521 agctggcaat agttttgtcg tcgaggtttt tctgccagtt gaacgacggc gagcagttat
     2581 aagccagcag tttgcccgga tatttcgcgt ggatagcttg tgcaaagcga cgcgccagtt
     2641 ccagatccgg cgtggaggtt tcacaccaga ccaggtcagc atatggcgca tacgccaggc
     2701 cacggctgat cgcttgctca atgcccgcat gagtacggaa gaagccttca ctggtacgct
     2761 cgccggtaat aaattcgctg tcatacgggt cgcaatcgga ggtgatcaga tccgcggcat
     2821 cggtacgagc aaccagtagg gttggaacgc ccgtcacgtc agctgccaga cgcgccgcga
     2881 ccagtttctg aatagcttct tgagttggca ctaaaacttt gccgcccatg tgaccgcatt
     2941 tcttcactga cgccagctga tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg
     3001 ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca tcggcaacga
     3061 tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcccgcg gaccattgga
     3121 tctgatccgc acggcggaaa gtattgttga tccgctccac cacagctggc accgagtttg
     3181 ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc gccgctacct
     3241 gccatcccga cagatagact gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc
     3301 cagtcagtgc accgaggctg ttgatgtagc ttttttcgac tcgccatgca gcagacgcca
     3361 cattctcgcc gcgcccagtt gcgccagggt gcattcagga ttgactgaat cgcgtaattt
     3421 caccacatct tccgcgctgt atgggcgagt aatgccttcc caacgcggtt gagtccactc
     3481 tttctgtaat tcttcaattt gttgtgtacg ggttttcatg tgcagatgct ccatagttat
     3541 gtggtggttt acgctaacag gcagtagcct ggcagggtca ggaaatcaat taactcatcg
     3601 gaagtggtga tctgttccat caagcgtgcg gcatcgccaa aacgcccctg ggagaaacgt
     3661 tcttcgccca gttcgctggc aatgactttc atctcttcgc ccagcatctg gcggaacaag
     3721 gctttagtta ccggtttgcc attgctcaac attttttgat gatggatcca ctgccagatt
     3781 gaggtacggg aaatttcagc cgtcgccgca tcttccatca ggccataaat cggcacacag
     3841 ccgttgccag agatccacgc ttcgatgtac tgcacggcta cgcgaatatt tgcgcgcata
     3901 ccttcttcgg tgcgttcacc gtcacaaggt gccagtagct gatcggcagt aatcggcgcg
     3961 tcttgttcgc gcatcacttc aagctgattt ttacgggagc cgagaatgtc gttgaatacc
     4021 gccattgccg tatccgcaag gcctgggtga gcgatccatg tgccatcgtg accgttattg
     4081 gcttccagcg atttatccgc ttttactttg ttgagcacct ggttattgtg ctcttcatct
     4141 ttgctcggaa taaacgccgc catgccgccc atcgcaaaag caccgcgttt atggcaggtt
     4201 ttaatcaaca ggcgtgagta agcattcagg aatggtttat ccatcgtcac tgcctgtctg
     4261 tctggcagga cgcgatcggg atagtttttc aacgttttga tatagctgaa gatgtaatcc
     4321 caacgaccgc agttcagacc aacaatatgg tcacgcagcg cgtgaaggat ttcatccatc
     4381 tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga
     4441 ttaaagcgat cttctgcata gctgaagact tcgctccacc aggccgcttc ctgccaggac
     4501 tgggttttcg gcagatagaa atagggacca ctgccctttg ccaacagagc ctgatagttg
     4561 tggaagaaat agagcgcaaa atcaaacagg ctgccgggga ttgcctcacc acgccaggtg
     4621 acatgttttt ccggcaagtg cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc
     4681 ttgagctggt aaattttgcc tgcttcattg gtatagctga tggtaccgtt aactgcatca
     4741 cgcaggttaa tttgcccgtc gatcactttg ttccagtccg gggccagtga atcttcgaaa
     4801 tcggccataa agactttcac attggcgttg agcgcgttga tcaccatctt gcgctctacc
     4861 gggccagtta tctctacgcg gcggtcttct aagtccgcag gaatcccgcg aattttccaa
     4921 tcagcatcgc aaatggaagc tgtttccgaa ataaaatcag gcaacgttcc gttatcaata
     4981 tcttgctgct gctgaatgcg cgctgccaga agtttatttc gttgtggcgt aaaatgcgtc
     5041 accagctcag tcagaaattc taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca
     5101 tacggccttg tgaaagccag ttcatcggtt gttgttgcct gttcagtcat cgtgcagctc
     5161 ctcgtcatgg atccgaaaac ttccccactg tgaacgaagg atcgttgtgc gcttttcgtt
     5221 cagcacaact aagactactc atttaaaatg caaaatcaaa aacaatttcc attttaattg
     5281 aaaatgcttg ataacttatt gataacaatg tgaataaaat ttattcacct cctgaggtgc
     5341 gtttcggaaa taaaaaaggc acccgaaggt gcctgaggta aggtgctgaa tcgcttaacg
     5401 atcgactatc acagaagatt aatccagcgt tggattcatg tgccgtagat cgtatggcgt
     5461 gatctgatag acgtaatagt tgagccagtt ggtaaacagc aaattaccgt gactacgcca
     5521 gctcgctcgc ggtgtatttt gcggatcatt gtgcgggaaa tagttatacg gtacttccgg
     5581 gtctagtccg gcttccacat cgcggaaata ttcctgcgcc agcgtttgcg catcatattc
     5641 gggatggccc gtcacaaagg caatgcgctt atctttgctg gcaaacagat atgcatcccc
     5701 ttcttccgtc tctgccagaa tttccagatc ggtgtaatca cgaatcaacg ctgccggaaa
     5761 gtcagcatag cgcgaatgtg gtgccaggaa tgaatcatca aagccacgcg tcagaagcgc
     5821 atgaggatgg agaatatgat gctcgtaaac gccagagagt ttgtcggtgc gagtttgctt
     5881 aggaatgccg tagaggatat tgagcgcggc ctgtaccgcc cagcagacaa acagcgtcga
     5941 ggtgacgtga tctttcgacc actccagcac ctgtttgatc tgcggccagt aagcgacatc
     6001 attaaactcc accaggccca gcggcgcacc ggttacaatc agaccgtcaa agttctgttc
     6061 ctgaatatct tcaaagttac agtagaagtt gttcagatgc tctgcgggcg tgttgcgcga
     6121 ttcacgggaa tcgatgcgca acagctgaat atcgacctgc aaaggtgagt ttgaaagcag
     6181 gcgcagaaac tgattttcag tttcaatctt cttcggcatc aggttaagga tcagaacctt
     6241 aagtggacga atttcctgac cagacgcacg agaagttgtc atcacaaaga cgttttcttc
     6301 acgcaagaaa ttgacggcgg gtagctcgtc cggcacacga atcggcataa cctgattacc
     6361 tcactacata cacttatacg tttagacatc cagatagctg aagataacca gaaaatttgc
     6421 caatgtcgag cctgcatgtt gaaggtgaga aagtttcaag gaaaagacta cgttagaata
     6481 taagaatgac taaaaggaga gaaaatggtt attagtattc gccgctcacg gcatgaggaa
     6541 ggggaggaac tcgttgcgat ttggtgtcga tctgtcgatg ccacacacga ttttttatca
     6601 gcagagtatc gggccgagct ggaggtgcta gctagttcgt tccttcctgc cggaagcgcc
     6661 gttgtggatc gcggttaatg aacgggatca gccggttggg tttatgttgt taagtgggca
     6721 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
     6781 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
     6841 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
     6901 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcccc
     6961 tgccgtgcct gcccaggctg gagaagacgc gccagcgtcg cacccggcat ttttt
//
LOCUS       DAJVTY010000185         6982 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVTY010000185 DAJVTY010000000
VERSION     DAJVTY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.185"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..287)
                     /locus_tag="OQQ66_003617"
     CDS             complement(<1..287)
                     /locus_tag="OQQ66_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1906383.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRL"
     gene            complement(281..1519)
                     /gene="preT"
                     /locus_tag="OQQ66_003618"
     CDS             complement(281..1519)
                     /gene="preT"
                     /locus_tag="OQQ66_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1906384.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1916..2155)
                     /locus_tag="OQQ66_003619"
     CDS             complement(1916..2155)
                     /locus_tag="OQQ66_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1906385.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2158..2877)
                     /gene="sanA"
                     /locus_tag="OQQ66_003620"
     CDS             complement(2158..2877)
                     /gene="sanA"
                     /locus_tag="OQQ66_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1906386.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3027..3911)
                     /gene="cdd"
                     /locus_tag="OQQ66_003621"
     CDS             complement(3027..3911)
                     /gene="cdd"
                     /locus_tag="OQQ66_003621"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1906387.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4041..4736)
                     /locus_tag="OQQ66_003622"
     CDS             complement(4041..4736)
                     /locus_tag="OQQ66_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1906388.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMHIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4733..5131)
                     /locus_tag="OQQ66_003623"
     CDS             complement(4733..5131)
                     /locus_tag="OQQ66_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1906389.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5370..6320
                     /gene="dusC"
                     /locus_tag="OQQ66_003624"
                     /pseudo
     CDS             5370..6320
                     /gene="dusC"
                     /locus_tag="OQQ66_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(6708..6791)
                     /gene="yohP"
                     /locus_tag="OQQ66_003625"
     CDS             complement(6708..6791)
                     /gene="yohP"
                     /locus_tag="OQQ66_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1906390.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         1821 a         1784 c         1726 g         1651 t
ORIGIN      
        1 ttcagccgac gcagggcggc cagattctcc tccagcggat gctcggcaat ctgctccata
       61 tttttgaagc cgataaaacc ggtatcttct ttcaccagat gatcaaaacg cggcgagact
      121 tcgttggcga taaaaaagcc gatcgtttta aacaccacac cgccccaacc tgtgtcgtag
      181 gctttggcac acatctcata gcagttgcct accggcgaag aagagaggca gaacgggttg
      241 ggaaacttca cgccgcaaaa agtaatcgaa agatctttcg ttaacatgag caagctccct
      301 ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt tttcacggca tagaccatgg
      361 ttttgtcacc ctcaacaata tcgccagcag caaagacttg cgggtcgcgg gtctggtaat
      421 tttgtgtttt gatggtgtta cgctgcggct ctaactccgc aaaggcatct agtctggcat
      481 gctgaccgac ggcgagaata attttatctg ccgccatcgt cagttcgccc gataaccgta
      541 catgcttaaa cgtgacttta ttgccttcca cggctactgg cgtgaatcca tcgatgatcg
      601 aaacgcccag ctcccgggcg ctggtaaact ctttttcgct tgccggaaac tcatctaact
      661 cttcacgcgc tacgcaagtt accgcctgac agccgagaac tttcagcgtg ctggctacgt
      721 ccatcgtgac atcaccgccg ccgataatta atgcgctttg tggaatgctg atatcgcctt
      781 gcgcctgtcg tgcacgttgc aagaagtcga cggcaatctc aacgtcacta tgctcaaaca
      841 gcggtagccc ggaaccgctt gataacccca cggtgaccag taccgcgcgg ttttctgctt
      901 tcagctgctc aagggtgagt gtgttaccga cttcgttgtt gcacttaatg gtcacgccca
      961 ttttttcaat acgggcgatc tctgcatcca gtactgattg cggtaaccgg aattgcggaa
     1021 tgccgttacg caaccagcca ccgggttgtg cttctttctc atagatcgtg acgtcataac
     1081 cctggtttgt cagtgtcaca ctggcctgca atccggcagg acctgcgcca ataatcgcga
     1141 ctttgccgag cgttttagta ccgggctgat aaatttccat cccggtttgt tgttcaaaat
     1201 cagtaacaaa acgctgtaag cggccgatat caatgggagc atcaacaccg gcacgggtac
     1261 aaccgctttg gcataatttc tccgtcgggc acactctggc acaaacggca ccgagggcat
     1321 tattttcgcg aattgtctcg gcagcgcctt taaaattacg aaagtagatt gagcgaataa
     1381 atttccccgg atcggtctgt gccgggcaag cctgactaca gggagcgtcg tgacataata
     1441 aacagcgaga ggcttcttta atcgccagta acggcgtaaa agccggagtg agttcatcca
     1501 gataattttg ttgcggcatg tgatcttcct taatgctatg agtaattaag gcgaacgcta
     1561 aatatactcg tcatacttca agttgcatgt gctgcgtctg cgggttattc ggcccatcca
     1621 tgggcctcac cccttatggg gccgctgcag gcagcgttca aatccgctcc atgcggattt
     1681 gtcgctcacc ccagtcactt acttatgtaa gctcctgggg actcactcac ttgtcgcctt
     1741 cctgcaactc gaattattta gagtataaat attgcgtgct gatattaagg ctatgcgcgt
     1801 ggggaaattt atactgtgaa aggattcaca aatagctgcg gttcagaatg ttatatccgc
     1861 agcttattga gtgaagtaat aattattctc aataattaac agaaaagaga aaatattaac
     1921 gatagaaaat aagataaaca atcatgccga tagataaaat tccgaagccg atatatgcca
     1981 ccatataagc gaaaaagagt ccgggatttt ttgctcgcca gacatacacc ggttccggtg
     2041 catttttaac ccgtttatca atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac
     2101 aaaatatcgg aataaggaaa aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta
     2161 ctttccttgt ttcttttgta attcaagtaa ctgttcgggt gtgacggcgg gatacccctg
     2221 cgcatcttcc ggcacctggt gcatagccgg aatagggacc agcggcccta aaaaacgcgg
     2281 ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa cgggcggcaa attcgcgaat
     2341 acgtactgac agcatatctt tcggtgacgg tacggcataa cactgagcct gaatccccat
     2401 atgcagcgca ataaataatg cccgctcaca gtggaaacgt tgggtgataa taatgaaatc
     2461 attagtatcg aaaactttgc gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta
     2521 atcgagaaca atatctgatg ggtcgacacc agcagcgatt aaatctttgc gcatggtcat
     2581 cggctcatta taactttgca atgcgttatc gccgctcagt aatagataat ttaccttacc
     2641 gctgttatag gcattaatcg ctccttgaat gcggtagcga taatactgat taattacgcc
     2701 agtacgataa tattttgctg ttccgagcac cacaccgacc tggcggtagg ggagatcctg
     2761 caattcgtcg tagatataag gcgcggtttt ccagctcatc cagcgatcga ggccgagcac
     2821 agtcaacaac agcaagccga tcaggactaa caggctgagg aacacgcgct ttaacatgca
     2881 ggagactcga tctaaggaaa atttccatca ggctacttta cccgctggtt ttgcgcaaga
     2941 aacggcaaac tgtttgccgg gaatttcgac accaggagag ggctggcaag ccggacaagg
     3001 cgttcacgcc gcatccggca ccaggcttaa gcgagaagca ctcggtcgat actgtgacag
     3061 ccgagagctt tcagcgttgc ggaggtggca tcccactgaa tcaacggcgc atcggctttt
     3121 tctgccagaa ccgcgcgctg gatatccggg taatcataac ccttgagatt caacagaatt
     3181 aacgctccct gcaacggtgg cagagtcggg ttgaatgcgg cgttttcagc gtagctgcca
     3241 ctgaaaatac gaccgtcttt acattccagc gcgacaccgc ttggcgactt actgtaaggc
     3301 atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca
     3361 tagccgtgat cctgctcgtc catcagcagc gttttaatct ccagatcttt cggcccaaag
     3421 gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc ccggcagatg aatacgcaga
     3481 tccagaccgc tgttcagttc attcataaac tgacggcagt gaccacaagg cgtgtagtta
     3541 acggtgatgg ctgcaagcgc tttttcacca ctcaaccagg cgtggctgat cgcgctttgt
     3601 tcggcatgaa cggtttgctg cattgtcgca ccaataaatt ccatattggc accgaaatac
     3661 caggttccgc tcacaccgcg cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc
     3721 gcacaggccg ctgccagcgg aagtagtgcg aatgccagcg cgtcttcgtc cagccccgtt
     3781 gcgctcttca gcgatgagac ttgctccccg gtcaacaaag cggggaagta cttgtctgcc
     3841 agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt gggcaaaagc ggtttgaaaa
     3901 cgtggatgca tggcgttgcc tcataatcat gtaatgggat aacagtttac gaacccgtta
     3961 tggctttata tgtgatctga atctcattaa ttatgcaacg cgtgaaactt acatcaaaaa
     4021 tgcgcgacgc atcgcaaatt ttagcccatt actgccagaa taatcgggaa aaggaacggt
     4081 gcgatcagcg aagtaattat cccgcataac accagcgcta gcgaactaaa tgcaccttcc
     4141 tgataatcca gctcggcgca gcgcgccgta ccgagggcgt gcgaggcagt ccccattgcc
     4201 agaccgcgcg cagctttggt acgaatatgc atcgcattaa gcaatgtatg gccaaatacc
     4261 gcgccgagga tgccgacgaa aatcacgcaa actgcgctga ttgccggaat accgccaata
     4321 ctgccgccaa ccgccattgc aataggcgtg gtgactgatt tcggcaggat tgacgcggcg
     4381 atttccggtg aagcgcccat caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg
     4441 ctgccgataa aacagatggt gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc
     4501 tcatataaag gataggccag cgcgacgacc gccggttgca gcagatcgtt tagcacttcg
     4561 ctacctttaa agtagctgtc gtaagagatg ccggtcagca ttaaaaaagg gatgatcacc
     4621 accattgcta ccagcaacgg gttaagcaag ggaaacttat accgtgccgc cagtttgcgg
     4681 gcggcaaaaa agacgatcaa tgtcagtggt aatgaccacc agatattagc catcattctt
     4741 ctgatccttt ctgacctacg actttacgtt caccgtgcac cagttgcgaa ctccagctca
     4801 ccaccagaaa aaccaccagt gtactgacag cacaggaaac cactaccggg ccaaactgtg
     4861 cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat cggcacaaac aatagcgcca
     4921 tatagcgaat cagtacgtag caccccggat tgacccattt tgccggaaga atttgcaagg
     4981 ccagcaggac aaacaggatc agcatcccga tgatgctgcc cggaatggtt accggtagca
     5041 gggaagcaat gaaaatgcct gcatacaggc aggcataaat caggacgaaa gcgcgtaaat
     5101 attgccagat aatgttcagt gtcttactca taatcaacac cccagatgag atgaattcat
     5161 catacaatta attcgtaaaa cgtgctaccg atcacattaa gaaatcagcg cttatttttt
     5221 cgctccccgg cattacgcaa tgcgcaacga actcaggcat acatcaagca tagagaggga
     5281 ttacagtttg cagcaatccc tctttgcaga cctttaatcc gttgaccata ggcgctaaca
     5341 tagcgcctca tttttttgcg ggtgaagata tgcgtgtgtt actggctccg atggagggtg
     5401 tgcttgactc tctggtgcgt gaattgctga ccgaagttaa cgactacgat ctgtgcatca
     5461 ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa agtttttcat cgcatttgcc
     5521 ctgagctaca aaacgccagc cggacaccat ccggtacgct ggtgcgcgtg cagttgttgg
     5581 gtcagttccc acaatggctg gcagagaatg ccgcccgtgc ggtggaatta ggttcctggg
     5641 gcgtggatct caattgcggc tgcccgtcga aaacggtcaa cggtagcggt ggtggggcga
     5701 cgttactcaa agatcctgaa ctcatctact agggtgcaaa agcgatgcgt gaagctgtac
     5761 cggcgcattt gcccgtcagc gtgaaagtgc gtctgggctg ggacagcggt gagaagaaat
     5821 ttgaaatcgc cgatgcggtt caacaggctg gtgctacgga gctggtggtg catggacgga
     5881 caaaagagca gggttaccgc gcggagcata ttgactggca ggcgattggc gagattcgcc
     5941 agcggctgaa tattccggtg attgccaacg gtgaaatctg ggactggcag agtgcgcaac
     6001 aatgtatggc gatcagcggc tgcgacgcag tgatgattgg tcgcggggcg ctcaatattc
     6061 ccaacctgag ccgggtggta aaatataacg aaccgcgaat gccgtggccg gaggtggttg
     6121 ctttgctgca aaaatatacc cgtctggaaa agcagggcga taccgggtta tatcacgttg
     6181 cgcggattaa acagtggttg agttatttgc gtaaagaata cgatgaagca acggaattat
     6241 ttcagcatgt tcgggtgttg aataattccc ctgatattgc aagggctatt caggcaattg
     6301 atatcgataa gctgcgataa tagtggaatc gcagtttatc agagacaatg aatcgcttat
     6361 tcagcttcat catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc
     6421 aaggagcgcg cagggggagg acaatcgcct cctccccctg cccccccggg ctctggcgaa
     6481 caaaatcgcc gctgcgcggt accttcagct tatccccttc ggctaccggg tcgggcgcga
     6541 ggtaacgtcc ttgtaagacg cgccctcagc ccacatccat gtgggctgcc ccggccttcg
     6601 gggaacgctt cagcgatttt gacgccacca acaccggagc tgtttgcaaa tcaagtacac
     6661 aaaatatcta gttcctgacc tgacaactta tatgccatat ctgattttta aaatatcatc
     6721 ttaaatacgc cagtcaccac cagtagccca atcaggaaaa taatcaatac agcccagagt
     6781 ataattttca ttggcgttcc tttatttgtg agtgttgaat aagtatagga aaaaacgaag
     6841 ccctttgccg ttatccataa acgattaaga taaatcggaa gatatcattc acttagtcta
     6901 tatttttctg ggtatgtcgt tttattgcta ctgatgtatt gccacgcgaa actaaaaaat
     6961 atatcgcaac acacggtaat ct
//
LOCUS       DAJVTY010000186         6923 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVTY010000186 DAJVTY010000000
VERSION     DAJVTY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.186"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            600..884
                     /locus_tag="OQQ66_003626"
     CDS             600..884
                     /locus_tag="OQQ66_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1906391.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            872..1357
                     /locus_tag="OQQ66_003627"
     CDS             872..1357
                     /locus_tag="OQQ66_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1906392.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1391..2569
                     /gene="ltrA"
                     /locus_tag="OQQ66_003628"
     CDS             1391..2569
                     /gene="ltrA"
                     /locus_tag="OQQ66_003628"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1906393.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2652..2806
                     /locus_tag="OQQ66_003629"
                     /pseudo
     CDS             2652..2806
                     /locus_tag="OQQ66_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2860..3342)
                     /locus_tag="OQQ66_003630"
                     /pseudo
     CDS             complement(2860..3342)
                     /locus_tag="OQQ66_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3598..4578)
                     /locus_tag="OQQ66_003631"
     CDS             complement(3598..4578)
                     /locus_tag="OQQ66_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1906394.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4578..5777)
                     /locus_tag="OQQ66_003632"
     CDS             complement(4578..5777)
                     /locus_tag="OQQ66_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1906395.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6099..6437)
                     /locus_tag="OQQ66_003633"
                     /pseudo
     CDS             complement(6099..6437)
                     /locus_tag="OQQ66_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6504..>6923)
                     /gene="istB"
                     /locus_tag="OQQ66_003634"
     CDS             complement(6504..>6923)
                     /gene="istB"
                     /locus_tag="OQQ66_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1906396.1"
                     /translation="AMGYEAVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMA
                     PRLLIIDEIGYLPFSQKEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTS
                     AMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1861 a         1573 c         1652 g         1837 t
ORIGIN      
        1 ggcggcatcc ggcatatagg cagcatgaac gcaacacagg ctctcgtacg gaacgtggga
       61 acctgtcgtc tcgatgctaa gggagcggcg cggagcagag gcactgcaag cgcctgagta
      121 ccgatgcgag acacaggggc agatggcttc gtagtagtga ggaagtgcct gtaatgggca
      181 tggagcgaag ggggtcagtc gaggcgtttg aggttaccaa tcgaagctat acccggtaat
      241 ggcgcaggtt gacctgcact tggccaagtg gattgcccgc aaacacaagc gagcgcgagg
      301 tagtctggtg cgtgcattcg agtggttact gcgaattcgt cacgactgcc cgacgctgtt
      361 tgcacactgg tgcttagctt acgatacttg aatggttcaa caacaagagc cgtatgacgc
      421 gagagtgtca cgtacggttc cgtgggagcg taggggggcg gtgcccctgc gccacccgat
      481 atcccctttg agtgcggtta cgtccacaga ggtaaatgcc gctgacgcgg catctgtaat
      541 ctgttacttt gtgtacatca tttgctatat attgtcaata tgctattgtt gtaaagcaaa
      601 tgatatacgg aggctttatg aaatcaggtg ttcagctcaa tctcagagca agagaatcac
      661 agcggatact cattgatgct gctgcagaaa ttcttcataa gtcgcgtaca gatttcattc
      721 tggaaatggc ctgtaaagct gctgaagatg ttattctcga ccggcgggtg tttaatttta
      781 atgacaggca atatgaagag ttcattgaaa tgcttgatgc tccggttgca gatgatcctg
      841 ccatcgaaaa attgctggca aggaaacctc aatgggatgt gtaactgcac cagaaccttt
      901 atcctcattt catcaggtcg ctgaatttgt cagcggtgaa gctgtacttg atgactggtt
      961 aaagcagaag gggctaaaaa atcaggcaat cggagccacc cggacttttg ttgtatgcag
     1021 aaaaggtacg caacaaatag tcggttttta ctcactggcc actggtagtg tcaatcatac
     1081 agaagctaca ggtaaccttc ggcgcaatat gccagacccc attcctgtaa ttatacttgc
     1141 acgcctggct gtagatgtgt catttcgagg aaaagggctt ggcgcggatt tacttcatga
     1201 tgcagttcgt cgttgttaca gggttgccga aaatatcggt gtgcgtgcaa ttatggttca
     1261 tgcgcttacg gaaaacgcaa aacagtttta tattcatcat ggttttaagc catcaaaaac
     1321 tcaggtgcaa acattgtttc ttaaactccc tcaataggga gtaatgcact ggtctgggca
     1381 tcatatctgg atgtgcgtcg caacaaggga gccccccgga tgcgacgggc agactttgaa
     1441 aatgttcgac cagcaacgtg atggcaatct atacaagatc tggaaccggc tatgttcggg
     1501 aacatggttt ccgccaccag tgctggaaaa acggatcccg aagtcgaatg gcaaggaacg
     1561 cattctgggg atcccaacag tatccgatcg aattgctcag ggagcgataa aacttttcat
     1621 ggaagaaaag cttgacccga ttttccatgc tgattcatat ggttaccgac caggcaaatc
     1681 agctcatgac gctctgaaac aatgtgccat ccggtgctgg cgttatagct ggatactgga
     1741 agtggacatc agtgcgtttt tcgaccatgt gaaacatgat ctggtgctca aagctctgga
     1801 acaccacggg atgcctaaat gggtcatcct gtactgtcga cgctggatgg aagcgccaat
     1861 gcagagttgt gaaaatggag aattaataac ccgaacacga ggcactccgc agggcggggt
     1921 cattagtccg ctacttgcta acttgttcct tcactatgca tttgatttgt ggatggaaag
     1981 agaatatcgg ggggtaccgt ttgagaggta cgctgacgat attgtagtac attgttcacg
     2041 aatgagtgat gcaacaaggc taaagaacag gttatcggag cgcttctcgg aagtcgggct
     2101 ggtcctgaat gcagggaaaa cgaatatcgc ctacattgac acgtttaaaa ggagaaacgt
     2161 cgcaacgagt ttcacctttc tcggatatga cttcaaagtg cgtacgctga agaatttcaa
     2221 aggcgaacgg taccgaaaat gcatgccggg tgcgtcaaat gcagcaatgc gcaaaataac
     2281 agaaacaatc aagaagtggc gtatacatcg ctcaacagct gagagtttgc tggattttgc
     2341 gaggcgctac aatacgatag tgagaggctg gatcgagtac tacggaaagt tctggtccag
     2401 aaatttcaac tatcgactgt ggagtgcaat gcagtcacgt ctgctcaagt ggatgcagtc
     2461 taaatacaga ctttcgaacc ggaaggctca gcgaaagctg acgctggtaa ggaaggagta
     2521 tccgaagcta tttgttcact ggtatttact acgtgcatca aatgagtgat caagagccgt
     2581 atgacgcgag agtgtcacgt acggttccgt gagcagccgg ggagtgaaat tctctccggc
     2641 tgactcgact cgcgggttcc gacagcgatg acgaacatgc ggcggtgttg tactcgctga
     2701 tcggcacatg ccgtctgaac aatgtggaac cagaaaatgg ctgcgttacg tcattgagca
     2761 tatccaggac tggccggcaa accgggtacg tgatctgttg ccctagaaag ttgatctgag
     2821 ctctcagtaa atatcaatac ggttctgacg agccgcttac ttttggcgta attctgctgc
     2881 gcctttactg aacacaatgc cgtattcagc aagaagtcca cgaatgtgat ttatcagttg
     2941 tgtcctttcc attgaaacca accgacgcat attcaaaaaa gtttggacgt cctgttgctc
     3001 cagagttttt attggtacgg gtcgtatagt tccatgttgg caggcctcag ctattgcctg
     3061 tgcgtcgtta taatcatttt tctggcaccg ctgataagct ttaacatgtt gtgccggaag
     3121 caatattaca tcatggtcga gtttcctgat ttcccgggcc cagtaatgag atgccccgcc
     3181 agcctccatc gcaacagtca ttttaggctg ttgcaccagc cagttaagca gttgattgcg
     3241 agaaagtttg gtctttcctg cgggtttgcc atgagcattg atactgaaaa gataaaagtt
     3301 cgttttagcc agatcaatgc cggtgatttt ctgttcagac atggtatccc gcttactttt
     3361 gtggttaaag tttatccatc atggcgcaat tgaaggctgc cgtgaaagag ggggaagact
     3421 atctcatcgg ttactgttaa acggctaaga gaaactatct ttttcaagca aagaagatac
     3481 ggtgaaacag gcgatttcac cgtgaaatac tgttcaaaaa aaacgccaat aaaagcaaaa
     3541 tttcttttaa aacagccaat taaaaactgg tgagttgctg gcgaaaaact tgaattttca
     3601 cagcgaattc agattatctt ttagtacaag agcaatcata cgatctaact tatcctgaac
     3661 ttccaacggt aatctgccga attcatatga gaacgtacgt ccctttactc gttttcgagc
     3721 aaatttatct ttactatcaa atttccataa caaagttaca accgctttat cctttactcc
     3781 tgaatcaagc aatgaagaca tttccttggt tataagccgc ataatactgt ttttaacctc
     3841 gtcttcagaa agattgtcat cagcagtaat aagctcaata ctcggcgaga tattcttcac
     3901 aacttcatca atagatatat cacccagcct cagtcgttca cttaattcag ccagttgcct
     3961 gtaatcggcg tatgtcagct cagactgaac aggaaaaaga gacacaaaat ctttaggtac
     4021 ggacgctgct tggagggcac gagtcacctt agctgccgac aatccctctc tttccgcaat
     4081 ttgcttctgg ttcataccgg cctctttgag acgtactaaa cgcaacccaa tttcacgaat
     4141 attatgttca agtgacgtct gcaaatcttt cgccagatgc tgagcttcag aaacagtaag
     4201 ctcatcatcc gttacaagta cacgtaatcc aaccttgcat agcaaagctg ctgcccgacg
     4261 acgggaacca tccaaaatct caataagatc gccagtccga atcccaatac atggatagaa
     4321 ttgttgtaaa cgtatagttc tggtgatgtc ttttagtgag tccggcgtca gggctgtctg
     4381 ttcgcggcca ttcacctctt caaccacaaa agtttgagtg tccactttgt catgatcaac
     4441 cttgatctca gtaaattttg cctttcggcc agtattgagg gtaaatacat gaactggagc
     4501 cgatatttct tcagtattat tcaggatatt tgtattcagc gtacggccaa tagtcggtcg
     4561 gtgctttctg ttttccatca gtagttggcc cttataaatt caatacgatc aaaaactgct
     4621 ttagcgaaat cttctgctgc catacgtgca tttttcaaag cttcaccact gccaacataa
     4681 gtaacaggat tcgcagatat cacggtatca aacgactcgc cgcttctctc aaaaccatca
     4741 agacgtggca ttgaaacatc aagcatgtca ccaccgaaaa tctcttttgt caggctatgg
     4801 cagtatttat gatctgactt attcgccagt ttcgacatga agccaatatt tgcctgcaaa
     4861 cggcatgaac atccatcctg ttcaataatc tgtacaagtt caggtaaacg agccaaatat
     4921 ttaagcgtgg aatggaaatc aacttgtgct ggcggcaccg gtgtaaacat gatatctgcg
     4981 gcagcaatgg catttttcaa aaatgcgtcc agatgcgggc ctgtatcaat aaggatgaag
     5041 tcgaaatcat gcttcagctt atcaataata ttctcacgca atatcgcgtg tttgttttgt
     5101 cctaatagat gctcctcgca cagagtatcc cagtttgatg caataaaggc gtcgtcaata
     5161 gaagccggca taacataaac gccagggata actgacggaa cgataaaatc ttccagtaat
     5221 tcctcacgac tcacgttctg caacatagcc tgcggtgcgg ttgtatcaac cagccccacc
     5281 gcatgcaagt agttcagaaa catagtggct gatgactggg gatctaaatc aaggagcaga
     5341 atacgtaaat cttctgacag caggtgagga tgagcacgca gggcgtgagc tacggaaaca
     5401 ctactcacgg ttttagacac accgcctttt agactaccga tgaatatgga atatgcctca
     5461 ctataacgat cgcgatattt aggaatgcca cgatgtgcat agatatcgat aatgttctgg
     5521 atggttaatg catattttgt cgcggttcct gctgggcgtt tttcaaactg atagccctga
     5581 gcctccatct caccgactgc accatccacg cttgcgcgcg ttaattttgg aagcttagct
     5641 acagcagact ttgaatagac ttgataaaaa acatcagcct gaaactcctg cttttgctcc
     5701 tgaatctgtt ttgttagggc gagcaacatt ttatctgcac gctgcgcaac cttacttaac
     5761 tgctcaaaac tagtcattcc ccctccgtgc tatgtaggat tttaacttat tacaaaaaaa
     5821 actgcattag cagggtaatg catgcaagtg catattgtac atgcttatat taaaaatgcg
     5881 cgcattgctg atttattaat catcggaatg aatattactg taagatagta tgcctttccg
     5941 ggagaaagac gctgatttcc cccgaataat ccagttcacc accacactcc ggacaacagc
     6001 tttcttcagg ctccagatgg tgtatttcgc gggaaacatg ttccggtaac ggacggcgat
     6061 gacgtgactg tcgcaacagg cgggggtagt gcttccaatc agtagaacat gtgcttttca
     6121 atgaaggagc cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta
     6181 cgtgccagcc gcttgatgcg ggtgcggagg gtccggttgt tacgttcaat acgttgcgtg
     6241 aagattttgc cggtcaggtg tttctccttc ggcacttctc tggcgtagct gccccagtca
     6301 tcgctggtca ccatgccgat acagaacgga gtcaacaggg ctaacagttc ccgacaggtc
     6361 tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt gttgtacgca
     6421 taccagagcc agtgttgtgt caacgacgga tgaaaagtga tccacttata tctccaccaa
     6481 cggcccaata ttgatccacc gttttactca ggattagctt ctgctataac cccggccttt
     6541 cgtttctgtc tgagtcgata gctttctcct ttgatttgaa cgacatgtga gtggtgtaag
     6601 atacggccca gcatcgctga ggtcagtgct gcatcaccgg cgaacgtttg atcccactgc
     6661 ccgaacggta gattggatgt caggatcatt gcgctctttt cataacgttt agcgatgacc
     6721 tggaagaaca gttttgcttc tttctgactg aacggcagat agcctatttc atcaatgatg
     6781 agcaggcggg gggccattac tccacgctga agcgtcgttt tataacggcc ctgacgttgt
     6841 gccgtagata actgaagtaa cagatctgct gctgttgtga agcgaacttg gatacctgca
     6901 cggactgctt catagcccat cgc
//
LOCUS       DAJVTY010000187         6914 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVTY010000187 DAJVTY010000000
VERSION     DAJVTY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.187"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..376
                     /locus_tag="OQQ66_003635"
     CDS             <1..376
                     /locus_tag="OQQ66_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906397.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
     gene            376..723
                     /gene="tnpB"
                     /locus_tag="OQQ66_003636"
     CDS             376..723
                     /gene="tnpB"
                     /locus_tag="OQQ66_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1906398.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            743..1897
                     /locus_tag="OQQ66_003637"
                     /pseudo
     CDS             743..1897
                     /locus_tag="OQQ66_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1901..2060
                     /locus_tag="OQQ66_003638"
                     /pseudo
     CDS             1901..2060
                     /locus_tag="OQQ66_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2649..2947
                     /locus_tag="OQQ66_003639"
                     /pseudo
     CDS             2649..2947
                     /locus_tag="OQQ66_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3029..3166
                     /locus_tag="OQQ66_003640"
                     /pseudo
     CDS             3029..3166
                     /locus_tag="OQQ66_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3536..4213
                     /gene="ospD1"
                     /locus_tag="OQQ66_003641"
     CDS             3536..4213
                     /gene="ospD1"
                     /locus_tag="OQQ66_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1906399.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            4537..4647
                     /locus_tag="OQQ66_003642"
                     /pseudo
     CDS             4537..4647
                     /locus_tag="OQQ66_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4725..5039
                     /locus_tag="OQQ66_003643"
     CDS             4725..5039
                     /locus_tag="OQQ66_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906400.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            5321..5887
                     /gene="ipgB2"
                     /locus_tag="OQQ66_003644"
     CDS             5321..5887
                     /gene="ipgB2"
                     /locus_tag="OQQ66_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1906401.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(6149..6271)
                     /locus_tag="OQQ66_003645"
                     /pseudo
     CDS             complement(6149..6271)
                     /locus_tag="OQQ66_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6414..6914
                     /locus_tag="OQQ66_003646"
                     /pseudo
     CDS             6414..6914
                     /locus_tag="OQQ66_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT         2030 a         1500 c         1619 g         1765 t
ORIGIN      
        1 ccggcggcct aattttcctt atgaatttaa aatcgcctta gtggagcagt cactgcagcc
       61 cggagcctgt gtggcgcaga tcgcccgtga aaacggaatc aacgataacc tgctcttcaa
      121 ctggcgccat caataccgga aaggtggcct gctgccttcc ggaaaaaata tgcctgcact
      181 gcttcccgtg acgttaacgc cggagccgga taataaaatc ccggctccgg cacaggaacc
      241 agagcagata aatacaccgt ccgacagtct gtgctgtgag ctggttctgc cggccggaac
      301 tctcaggctt aaaggtaaac tgacgccggc gttattacag atacttatcc gcgaaataaa
      361 agggagcagc cactgatgat atctttccct gcaggttcgc gtatctggct ggttgcaggt
      421 atcaccgata tgcgaaatgg ctttaacggc ctggcctcaa aagttcagaa cgtcctgaag
      481 gatgacccgt tctccgggca tctgttcatc ttccgcggac gccggggtga ccagataaaa
      541 gtgttgtggg ctgacagtga cggactgtgc ctctttacca gacgcctgga gcggggccgc
      601 ttcgtctggc cggtcacccg tgacggcaag gtgcacctta ctccggccca gttatccatg
      661 cttcttgagg gcatcgactg gaaacacccg aaacgaacgg aacgcgctgg catccgcata
      721 taacccgttg taaagtgagg atatggacac ctcacttgct catgagaatg cccgcctgcg
      781 ggcactgttg cagacgcaac aggacaccat ccgccagatg gcgaaataca accgcctgct
      841 ctcacagcgg gtggcggctt atgcttccga aatcaaccgg ctgaaggcgc tggttgcgaa
      901 actgcagcgc atgcagttcg gttaaagctc agaaaaactt cgcgcaaaaa ccgaacggca
      961 gatactggaa gcacaggagc gaatcagcgc acttcaggaa gaaatggcgg aaacgctggg
     1021 tgagcaatat gacccggtac tgccatctcc cctgcgccag tcttcagccc gtaaaccgtt
     1081 acctgcctca cttccccgtg aaacccgggt catccgtccg gaagaggaat gctgcccggc
     1141 ctgtggtggc gaactcagtt ctctgggatg tgatgtgtca gagcagctgg agcttatcag
     1201 cagcgccttt aaggttatcg aaacacaacg tccgaaactg gcctgttgcc ggtgtgacca
     1261 tatcgtgcag gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct
     1321 tctggcccat gttgtcgctg gaaaatatgc aggctatctg ccgttatacc gccagtcaga
     1381 aatataacgt cgtcagggcg tggagctgag ccgtgccaca ctgggtcgct ggaccggtgc
     1441 cgttgctgaa ctgctggagc cgctgtatga cgtcctgcgc cagtatgtgc tgatgcccgg
     1501 taaagttcat gcggatgata tccccgttcc ggtccaggag ccgggcagtg gtaaaacaag
     1561 aactgcccgg ctgtgggtct atgtccgtga tgaccgtaac gccggttcgg aaatgcctcc
     1621 ggcggtctgg ttcgcgtact caccggaccg gaaaggtatc catccacaga atcatctggc
     1681 cggttacagc ggagtgcttc aggccgatgc ttacggtggt taccgggcgt tgtacgaatc
     1741 cggcagaata acggaagccc tgctgcgtat cggtgaactg tatgccatcg aggcagaagt
     1801 ccggggatgt tcagcagaac agcgtctggc ggcaagaaaa gccagagccg cgccactgat
     1861 gcagtcactg tatgactgga tacagcaaca gatgaaaatt cattcgctga aaatggaatg
     1921 tctccacgga gaacactatt atccatcggg aaatagtgcg ggcaacagtg tttagttata
     1981 tcgagtgcga ttacaatcgc tggcgacggc accgttggtg tggtggtctc agtccggaac
     2041 aatttgaaaa ccagagcctc acttacgact gtgtccatat tatgtgggta ggatcaataa
     2101 agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac
     2161 atgcttgaca acttaattag ggccccctct tccttttatg gtgtaattgt ttgattgaat
     2221 acttccgggg atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta
     2281 ttagtggaat aagaacaata gtttatccaa actgccaagc ccctttatta taagtaagat
     2341 ctggcactgg aatattctca aaaggactaa catcatcacc acgaccttca accaaccata
     2401 aaaaacaata ctattttagg tgatgtatta aattagtaga atatttattc ttctataaaa
     2461 gcactacccc aagaggttaa tcaggtcatc catgattatt tatcatatac taactatgtg
     2521 ggatttcaac gtgaaaactt attttcacta taatcttcag gattcgaaga tcataggagc
     2581 ctgtacataa gtttgtataa ttctctgatg ttgaactatt taatttgata tcaaatgaag
     2641 gtgaatttat gaatgaaaaa cagttgacat ctaataagtt gcgggcttta gctaatgaac
     2701 tagccaaaag tctcaaaaat cctgaagatc tcagtcagtt tgattggatg ctgaaaatga
     2761 aaccgtactc aatgctaata tgactcatca tcttgggtgc gagaaaaatc agcttagatc
     2821 tggcagcaat tcccgcaatg gttgcttaac gaagattatc actactggag atgaacctct
     2881 ggaaatccgt actctgcgtg atcgtaatgg cactttcgaa ccgcaacagc tgaagaaaaa
     2941 ccagccctag attactaaat ctgtcgggcg atggattaac tggttcaggc tgtctggtgc
     3001 tgaaggcctg aagtcactgc tgtcagtgat gtggccggct cttcatgaga caataacccg
     3061 taatcatcag tgccgttcaa tatggccact gttgaaaaaa gttcgtcact ttatggagac
     3121 ggttagccca cttcccggag aaaaacatag tctggccaaa gtgtagcggt attaggctca
     3181 catatttaga tgtagataag ccggagcagg cagttaaatc taggcagcat cacagaaatg
     3241 gacaacgccc cttaaggact ggcgtatggc aatgagctgc tttattatca agtttggtga
     3301 tcgccttgac ggtcacttct gagtgaaggc gcttgcacag aatggtaaac ggactcgatt
     3361 attgaatctt agggataaga tatatctacg atatgtcaat atacgaaatc gaaagaattt
     3421 attggtgaat atagccattc aatgtggcaa tggttttttt agattagcaa tagactaata
     3481 tctattacat tacaaaaata ggttattgtt tttttagcaa aaaggatgaa caaaaatgtc
     3541 aataaataac tatggattac atccagcaaa caacaaaaat atgcacctaa taataggcag
     3601 caatactgct aatgaaaata aaggaatgaa acgtaatatc attaacgtga caaataccgc
     3661 tatatcccac gccatcaatg aagaaaaatc agggggggga tatagtggtg tttctttcag
     3721 aaaattggcc aaaatacaga gcatatccat tccgacaaag aataataagg agtataaccg
     3781 ccataatttg ttttcattga tttggcatgg aaatgctgat gcagcgcgta aatacggtga
     3841 atcgctgttg gcagccgaaa tacccaaaga ggaaaaacta gaagttcttg cagcacgaaa
     3901 taatgctggg gaatctgctt tgttcatagc tcttcaagaa ggtcattccg ctgcgattca
     3961 agcttatgga gattttatta aaacttttga tttatcacca aaagaaacga ttaaactatt
     4021 ggatgtaaga gataatgagg ggttaccagg attatttctg gccgcaggga aagggaatat
     4081 cgaggctatg atggcatata taaatatatg ccatcatagt gggataaaac ttacagaaat
     4141 agcagacaga cttaacaata atgaacaaga catgtttaat attatttctg acaaaataca
     4201 agagttgttt taagtgtgct aaatagctgc aaagaattgc acttagaagg agctctcccc
     4261 gaattgtgtg atctgcttaa tttctaaaaa gaaacaaaca gttaactaga ctgagcgata
     4321 ctacccatag aaccagtttc ccatgacgaa cgacactgga cgcaaaaaca atcataaaaa
     4381 acacaataaa tgtaatgaac acatcccacc ctattaagta aagtgcactg tgatagagga
     4441 agggaaatac aggaaggtgt ggagccttgc agatttctgg catcatagtg cccaatacgg
     4501 aatgcataaa gccccctcag actggagact ccacaaatga aatatacagt gtttaattat
     4561 atcaagtgcg attacaatcg ctgacgacgg cacagttggt gtggaggtct cagtcctgaa
     4621 caatttgaaa accagaacct cacttaagcc tgtgttcata ttacgtgggt aggatcatac
     4681 ttttatagaa gctgtcagtg gctacgtccc agctaaatca cttcatgctc cagcgccaga
     4741 gaggcaaggt tgggtttgct cagctgccgg ttgattttgt tgcgattgaa ccggacagcg
     4801 cccagggtgt cggcaaaaga gcgaatctca ccaatcgttg tttcattatc cgcattaacg
     4861 atagcttcaa gaaacgccaa ggttttatcg aattcatcag caacagtggc tccgggcata
     4921 ccgtgaccgt ttatacaaaa aggcgttttc agcgtggagt tttcatgaat agcctcaaca
     4981 cgaatgtcgt gaagccagtg atgtatcggc tacgatcctc agctgatgcc agaccatagg
     5041 actgacacta cgaacagcaa ttttaatgac gtaaatcttc ataactacaa ctaacagggc
     5101 atcattatga atgatgccgg ttattttacc gctgttggtc ccttcattta gggactgccc
     5161 caagtttttt catactctct ttctcagaga gtttagtctc tgggattcca gacggtccat
     5221 cattgagcca agggagtaaa gaactcaaag catttacaga attgtttgaa tcagctaaaa
     5281 taatgacaat gttactattt tacatgacaa agtgattata atgcttggaa catcttttaa
     5341 taattttgga atcagtttat ctcacaaaag atatttttca ggaaaggttg atgagattat
     5401 ccgctgtaca atggggaaaa ggatagtaaa aatatcatca accaaaataa atactagtat
     5461 tttgagctct gtttcagaac aaatcggtga aaatattaca gattggaaaa atgatgaaaa
     5521 aaaagtctac gtatcccggg tggtaaacca atgtattgat aagttttgtg cagaacacag
     5581 ccgaaaaatc ggcgataatc tccgtaaaca gatatttaaa caggttgaga aagattatca
     5641 catttctcta gacatcaatg ctgcacaaag ttccataaat catcttgtat caggaagcag
     5701 ttatttcaaa aagaaaatgg acgaactttg tgaagggatg aatagaagcg ttaaaaatga
     5761 tacaacaagc aatgttgcaa atcttatttc cgatcagttt tttgagaaaa atgtgcaata
     5821 tattgatcta aagaaattac gtgggaatat gagtgattat attacaaatt tagaatcgcc
     5881 tttctgatga cagaacatcc cgagtaaaga aactaagaaa agtaaatacc caggaattgc
     5941 ctgagcggtg ctatatttct cctcgcaagt aagtcctaat atattgaatt gttgtatagc
     6001 cagtttttgt attttcctag ctcatggatg tgggatccct ctcccctttc tctgctcaaa
     6061 ggtaagcggc tcgtcagaat catattgata tttactgaga agtcagatca actttccaag
     6121 gcaacagatc gcgtacccgg tttgccggtc agtcctggat atgttcaata acgtaacgca
     6181 gccatttttc tggctccaca ttgttcagac ggcatgtgcc gatcagcgag aacaacaccg
     6241 ctgcatgttc gtcaccgctg tcggaacctg cgagtcgagt cagccggaga gagtttcact
     6301 ctccggctgc tcacggaacc gtacgtgaca ctctcgcgtc atacggctct tgatcactca
     6361 ttcgatgcac gtagtaaata ccagtgagca aattgatctt acccagcaat acggtcagtt
     6421 accgcgcaca tggcctgcct gtgtcagaaa atctgctgga gcaggatttt tacgccagtg
     6481 gtccgaacca gaagtgggcg ggagacatca cgtacttacg tacagatgaa ggctggctgt
     6541 atctggctgt ggtcattgac ctgtggtcac gtgccgttat tggctggtcc atgtcaccac
     6601 gcctgacggc gcaactggcc tgcgatgccc tgcagatggc gctgtggcgg cgtaagagac
     6661 cccggaacgt tatcgttcac tcggaccgag gaagtcagta ctgttcagca gattatcagg
     6721 cgctgctgaa gtggcataat ctgcgtggca gtatgagcgc aaagggctgc tgctacgata
     6781 atgcctgcgt ggaaagcttc tttcattcgc tgaaagtgga atgtctccac ggagaacact
     6841 ttatcagccg ggaaataatg cgggcaacag tgtttaatta tatcgaatgt gattacaatc
     6901 gctggcgacg gcac
//
LOCUS       DAJVTY010000188         6845 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVTY010000188 DAJVTY010000000
VERSION     DAJVTY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.188"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..646
                     /locus_tag="OQQ66_003647"
     CDS             <1..646
                     /locus_tag="OQQ66_003647"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1906402.1"
                     /translation="YKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQ
                     EGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHA
                     AFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAEL
                     LRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            737..1984
                     /gene="tnaB"
                     /locus_tag="OQQ66_003648"
     CDS             737..1984
                     /gene="tnaB"
                     /locus_tag="OQQ66_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1906403.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2116..3291
                     /gene="mdtL"
                     /locus_tag="OQQ66_003649"
     CDS             2116..3291
                     /gene="mdtL"
                     /locus_tag="OQQ66_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1906404.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3266..4225
                     /gene="yidZ"
                     /locus_tag="OQQ66_003650"
     CDS             3266..4225
                     /gene="yidZ"
                     /locus_tag="OQQ66_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1906405.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4382..5131
                     /locus_tag="OQQ66_003651"
     CDS             4382..5131
                     /locus_tag="OQQ66_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1906406.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5153..5719
                     /gene="yieF"
                     /locus_tag="OQQ66_003652"
                     /gene_synonym="chrR"
     CDS             5153..5719
                     /gene="yieF"
                     /locus_tag="OQQ66_003652"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1906407.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5773..>6845)
                     /gene="adeP"
                     /locus_tag="OQQ66_003653"
     CDS             complement(5773..>6845)
                     /gene="adeP"
                     /locus_tag="OQQ66_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1906408.1"
                     /translation="PAMGLNAFFAFVVVQAMGLPWQIGMGAIFWGAVGLLLLTIFRVR
                     YWMIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANSETLVSIGNLTSHSVLLGILG
                     FFIIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGIVSAPPSVMTVVGHVDLAGSF
                     NLGLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGKFPRMKQALYVDSISSVTGSF
                     IGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLSPLAGMVPGYAAAGALIYV
                     GVLMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIALGFISYCVMKIGTGRLRD
                     LSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1620 a         1748 c         1735 g         1742 t
ORIGIN      
        1 ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg tgccgatggg
       61 cggcttgctg tgcatgaaag acgacagctt ctttgatgtg tacaccgagt gcagaaccct
      121 ttgcgtggtg caggaaggct tcccgacata tggcggcctg gaaggcggcg cgatggagcg
      181 tctggcggta ggtctgtatg acggcatgaa tctcgactgg ctggcttatc gtatcgcgca
      241 ggtacagtat ctggtcgatg gtctggaaga gattggcgtt gtctgccagc aggcgggcgg
      301 tcacgcggca ttcgttgatg ccggtaaact gctgccgaat atcccggcag accagttccc
      361 ggcacaggcg ctggcgtgcg agctgtataa agtcgccggt atccgtgcgg tagaaattgg
      421 ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc cggctgaact
      481 gctgcgttta accattccgc gcgcaacata tactcaaaca catatggact tcattattga
      541 agcctttaaa catgtgaaag agaacgcggc gaatattaaa ggattaacct ttacctacga
      601 accaaaagta ttgcgtcact tcaccgcaaa actgaaagaa gtttaattaa tactacagag
      661 tggctataag gatgttagcc actctcttac cctacatcct caataacaaa aatagccttc
      721 ctctaaaggt ggcatcatga ctgatcaagc tgaaaaaaag cactctgcat tttggggtgt
      781 tatggttata gcaggtacag taattggtgg aggtatgttt gctttacctg ttgatcttgc
      841 cggtgcctgg tttttctggg gtgcctttat ccttatcatt gcctggtttt caatgcttca
      901 ttccgggtta ttgttattag aagcaaattt aaattatcct gtcggctcca gttttaacac
      961 catcaccaaa gatttaatcg gtaacacctg gaacattatc agcggtatta ccgttgcctt
     1021 cgttctctat atcctcactt atgcctatat ctctgctaat ggtgcgatca ttagtgaaac
     1081 gatatcaatg aatttgggct atcacgctaa tccacgtatt gtcgggatct gcacagccat
     1141 tttcgttgcc agcgtattgt ggataagttc gttagccgcc agtcgcatta cctcattgtt
     1201 cctcgggctg aagattatct cctttgtgat cgtgtttggt tctttcttct tccaggtcga
     1261 ttactccatt ctgcgcgatg ccaccagcac cactgcggga acgtcttact tcccgtatat
     1321 ctttatggct ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct
     1381 gattatttgc tatggaaaac gcaaagataa gttaatcaaa agcgtggtat ttggttcgct
     1441 gctggcgctg gtgatttatc tcttctggct ctattgcacg atggggaata ttccgcgcga
     1501 aagctttaag gcgataatct cctcaggcgg caacgttgat tcgctggtga aatcgttcct
     1561 cggcaccaaa cagcacggca ttatcgagtt ttgcctgctg gtgttctcta acttagctgt
     1621 tgccagttcg ttctttggtg tcacgctggg gttgttcgat tatctggcgg acctgtttaa
     1681 gattgataac tcccacggcg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacctgc
     1741 gttgttgtat ctgatcttcc cgaacggctt tatttacggg atcggcggtg ccgggctgtg
     1801 cgccaccatc tgggcggtca ttattcccgc agtgcttgca atcaaagctc gcaagaagtt
     1861 tcccaatcag atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt
     1921 tggtataacc gtgattttgt gctggttcgg caacgtcttt aacgtgttac ctaaatttgg
     1981 ctaaatcctt caagaagcca gccattcgct ggcttcttgc ctctcaggaa atcacttatg
     2041 tccaaatggc aactcgcctg atcctccttc accacgtatg ctttgcgtca ccttactatc
     2101 aggacgcttt agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat
     2161 cccgccggga ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc
     2221 agcgaagcgc agttgcatat tgcgttctcc gtatatctgg cggggatggc agctgcgatg
     2281 ttatttgccg gtaaagtggc cgatcgttca gggagaaagc cggtcgccat acccggcgcg
     2341 gcgctattta ttattgcctc ggtgttctgt tcactggctg aaaccagcac gttatttctt
     2401 gcaggccgat ttctacaggg gttgggcgca ggctgttgtt acgtagtggc gttcgccatt
     2461 ttgcgcgaca cgctggatga tcgacgtcgg gctaaagtgc tgtcattact caacggtatt
     2521 acctgcatca ttccggtgtt agcgccagtg ctcggacatc tgattatgct taaattcccg
     2581 tggcagagtc tgttctgggc gatggcaatg atgggcatcg cggtactgat gttgtctttg
     2641 tttattttaa aagaaacgcg cccagcgtcc cccgctgctt cggacaaacc acgagaaaat
     2701 agcgagtcgc tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt
     2761 tcggtgatcc tcactttcgt caatacatcg ccggtattgc tgatggaaat catgggtttt
     2821 gagcgcggag aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca
     2881 ttctccacgc catttgcgct gggaattttt aagccacgta cgttgatgat cacctcgcag
     2941 gtgttattcc ttgcagcggg gatcaccctt gccgtttcac cttcccatgc ggtttctctg
     3001 tttggtatca cactgatttg cgccggtttc tcggtaggtt ttggcgtagc gatgagtcag
     3061 gcgttaggac cattttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcag
     3121 gtttgcggtt cgtcactgtg gatttggctg gcagcggtgg ttggtatcgg cgcatggaat
     3181 atgctgatcg ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc
     3241 gcgcctggac gccccgttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct
     3301 gctgctctgt ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat
     3361 gaacgtgacc ccttcagcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga
     3421 cccgctcttt gtgaactcac cgctggggct gtcgcccaca ccgctaatgg tcagcatgga
     3481 gcaaaatctg gcggaatgga tgcaaatgag caatctgctg ctggataaac cgcaccacca
     3541 gacaccgcgc ggcctgaagt ttgagttggc ggcggaatcg ccgctgatga tgatcatgct
     3601 taatgcgctg tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aactacgtaa
     3661 ctgggattac gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg
     3721 tcgcgaaagc catcctcgct cgcgggagct gttaagctcg ctaccgttag ccattgatta
     3781 tgaagtgctg tttagtgatg taccctgcgt ctggttacgc aaagatcatc cggcactgca
     3841 tgaaacgtgg aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca
     3901 gagcgacacc tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gcacgattgc
     3961 tatgagcctg ccggaatttg agcagtcact gtttatggca gcgcaacccg acaatctgct
     4021 actggcgacc gcgccgcgct actgtcagta ctacaatcaa ctccatcaac tgccgttggt
     4081 tgctcttcct ctcccgtttg acgaaagcca gcaaaaaaag ctggaagttc cttttaccct
     4141 gctgtggcat aaacggaaca gtcataatcc gaagatcgtc tggttacggg aaaccattaa
     4201 aaacctttac gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata
     4261 aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc
     4321 catcattcca gtaaatgatt actcttatat tcataatgga ccattaagca tggagcgaaa
     4381 aatggcgact cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct
     4441 cccctcccag tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct
     4501 ggcgtccaga ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc
     4561 ggaaatcgtc accctgccga aaggtaaacc ggttttcagt gataaaaatt tgccttcgtt
     4621 ttctatttcc tatgccggga atatggttgg cgtggcgtta acaaccgaag gtgaatgtgg
     4681 cctcgatatg gaactacagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa
     4741 ccacaccttt tccagcaatg aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc
     4801 gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa
     4861 tgacgatccg cgcgatctgc agctgctgcc cattgccgga cgcctgaaat gtgctcatgt
     4921 aaatcatgta gaagcgttat gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac
     4981 gcccgcgatt gaaaagctca gtgtctggga gttagatggc aaacacagct ggaaaagcct
     5041 gccggatatt cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct
     5101 ctctaccgtg aagtcttttt cgccaaattg atagacaacc acaggagtca ttatgtctga
     5161 aaaattgcag gtggttacgt tactggggag cctgcgcaaa ggctcattta atggcatggt
     5221 tgcacgtacc ctgccgaaaa ttgctccggc gagcatggaa gtcaatgcgt taccttccat
     5281 tgccgacatt cccctgtatg acgctgacgt acagcaggaa gaaggttttc cagcaacggt
     5341 tgaagctctg gcggaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata
     5401 taactactcg gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga
     5461 tcaaccgctg gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg
     5521 cgcgcgctgt cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa
     5581 caagccggaa tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt
     5641 gattgatcag ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat
     5701 tcagcgagtt aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat
     5761 gcgctatcga ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac
     5821 gatgattacg cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta
     5881 ggagataaag cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc
     5941 ggtaataaac gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga
     6001 ggtcatcaac acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc
     6061 cgccagcggc gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag
     6121 accggtacga ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga
     6181 agtaccgata aacgaaccgg tcacggaaga gatactgtcg acatacagcg cttgcttcat
     6241 gcgcgggaat ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt
     6301 accggaggag tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag
     6361 gttaaacgac ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga
     6421 aacgatgcca ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat
     6481 agaaaccagc accgctgcgt gaatgttgcg cgaggccaga atagcaatga tgaagaagcc
     6541 gaggataccc agaagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccga
     6601 gttagcgaca atcacacctg cgtttttcag ccccatcatg ccgataaaca ggccgatacc
     6661 gctggtaata cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg
     6721 gaaaatggtc aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg
     6781 ccacggcagc cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc
     6841 aggag
//
LOCUS       DAJVTY010000189         6808 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVTY010000189 DAJVTY010000000
VERSION     DAJVTY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.189"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            30..236
                     /locus_tag="OQQ66_003654"
                     /pseudo
     CDS             30..236
                     /locus_tag="OQQ66_003654"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            981..2894
                     /locus_tag="OQQ66_003655"
                     /pseudo
     CDS             981..2894
                     /locus_tag="OQQ66_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3022..3744)
                     /locus_tag="OQQ66_003656"
     CDS             complement(3022..3744)
                     /locus_tag="OQQ66_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1906409.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3891..6518
                     /gene="gyrA"
                     /locus_tag="OQQ66_003657"
     CDS             3891..6518
                     /gene="gyrA"
                     /locus_tag="OQQ66_003657"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1906410.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6667..>6808
                     /locus_tag="OQQ66_003658"
     CDS             6667..>6808
                     /locus_tag="OQQ66_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906411.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVM"
BASE COUNT         1631 a         1676 c         1966 g         1535 t
ORIGIN      
        1 atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa gttacctttg
       61 atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga gctgttgact
      121 ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta cttaatgcag
      181 ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc ctgggccgca
      241 gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa gactgctacg
      301 gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac gttggctcca
      361 cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta aatatcgatg
      421 ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa ggtgctggtc
      481 agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg atggcgctaa
      541 ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac gcggcagatc
      601 tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg ctcgatctct
      661 ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa gtcagtggca
      721 gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc gacaatttgc
      781 atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc gacgggcagg
      841 taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc ggtacgctga
      901 tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt atctttaccg
      961 ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc tctacgacga
     1021 ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt gatgcggggg
     1081 tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag gatgagggta
     1141 gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt acaacgcagt
     1201 cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc tttccttatg
     1261 cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat caggatattc
     1321 agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg gttttgcgtc
     1381 tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc gatgggacgc
     1441 tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac cttgaaactg
     1501 acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat acgtccggtg
     1561 cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt gattatactg
     1621 gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta agcgatcaat
     1681 tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc attacaggga
     1741 ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat cccacgcagt
     1801 ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg ggagcgtatg
     1861 actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa gtaacgccgc
     1921 catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca cccgatccaa
     1981 tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat aacctgcggg
     2041 cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat ggtcagacgc
     2101 tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa ctggctcagc
     2161 aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc tggggcacgg
     2221 atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc gacagccgct
     2281 cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt gggctgacct
     2341 caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc tggctgcaat
     2401 acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt taccattcgt
     2461 cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt ggtgtggtga
     2521 ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt accgccgcta
     2581 accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg ggtttacaca
     2641 gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat tatcacgatc
     2701 cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg gtgaagcgac
     2761 ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc ctgcgcggca
     2821 gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt ttgtcgatga
     2881 cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt aaacgcctta
     2941 tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat
     3001 tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt tcacatccac
     3061 gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag ttatatgtcg
     3121 ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa taaacttctt
     3181 cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa ccaccgccat
     3241 cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc ctggtttcac
     3301 cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca gcatctccat
     3361 gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca cggtttcctg
     3421 cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct gcaatggctc
     3481 aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct cggccagaat
     3541 gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag cacgctcggc
     3601 aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac cttcaagatc
     3661 ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta cgttatggtt
     3721 ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa gacaaacgag
     3781 tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc gcggctgtgt
     3841 tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag atgagcgacc
     3901 ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc tatctggatt
     3961 atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc ctgaagccgg
     4021 tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac aaagcctata
     4081 aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat ggtgactcgg
     4141 cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac atgctggtag
     4201 acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg cgttatacgg
     4261 aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa gagacggtcg
     4321 atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca accaaaattc
     4381 ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc aacatcccgc
     4441 cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat gaagacatca
     4501 gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg gcaatcatta
     4561 acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg tatatccgcg
     4621 ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc gtccacgaaa
     4681 ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg gtaaaagaaa
     4741 aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt atgcgcatcg
     4801 tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc tactcccaga
     4861 cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt cagccgaaga
     4921 tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa gtggtgaccc
     4981 gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt gaagcattag
     5041 ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg ccgacgcctg
     5101 cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt gccgcgatgc
     5161 tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag ttcggcgtgc
     5221 gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat ctgcgtttgc
     5281 agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag ctgctggatc
     5341 agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa gtgatccgtg
     5401 aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa atcaccgcca
     5461 acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta gtgacgctct
     5521 ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag cgtcgtggcg
     5581 ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga ctgctggtgg
     5641 cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat tcgatgaaag
     5701 tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc aacctgctgc
     5761 cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt gaagaaggcg
     5821 tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc accgagttca
     5881 accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat gagctgatcg
     5941 gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa ggtaaagtgg
     6001 tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt gttcgcggta
     6061 ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc gatggcgcaa
     6121 tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa tacccaacca
     6181 agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac ggtttagttg
     6241 ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat gccggtacgc
     6301 tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag ggcgtgatcc
     6361 tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct gaaccggttg
     6421 acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat gaaatcgctc
     6481 cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc atgccaaaag
     6541 ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg cttttgtcgt
     6601 attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata cacaaggaat
     6661 gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct tgtaggtttt
     6721 ggcgcaatag tactgctttc cgctggcgtc tgggcgttgc aatatgctgg cagtgggcca
     6781 gaaaaaacgt tgtcgccgct ggtgatgc
//
LOCUS       DAJVTY010000190         6785 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVTY010000190 DAJVTY010000000
VERSION     DAJVTY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.190"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..158
                     /locus_tag="OQQ66_003659"
     CDS             <1..158
                     /locus_tag="OQQ66_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906412.1"
                     /translation="ILQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRA
                     GGYYEGA"
     gene            145..732
                     /locus_tag="OQQ66_003660"
     CDS             145..732
                     /locus_tag="OQQ66_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906413.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            729..1130
                     /locus_tag="OQQ66_003661"
     CDS             729..1130
                     /locus_tag="OQQ66_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906414.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1141..1881
                     /locus_tag="OQQ66_003662"
     CDS             1141..1881
                     /locus_tag="OQQ66_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1906415.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1940..2326
                     /locus_tag="OQQ66_003663"
     CDS             1940..2326
                     /locus_tag="OQQ66_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1906416.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2335..2663
                     /locus_tag="OQQ66_003664"
                     /pseudo
     CDS             2335..2663
                     /locus_tag="OQQ66_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2635..5676
                     /locus_tag="OQQ66_003665"
     CDS             2635..5676
                     /locus_tag="OQQ66_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1906417.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5676..6005
                     /locus_tag="OQQ66_003666"
     CDS             5676..6005
                     /locus_tag="OQQ66_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906418.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6005..6785
                     /locus_tag="OQQ66_003667"
                     /pseudo
     CDS             6005..6785
                     /locus_tag="OQQ66_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428152.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
BASE COUNT         1631 a         1558 c         2203 g         1393 t
ORIGIN      
        1 atattttaca gctgtgtcgc aatgattcgc tgatgattgg ccgggagtct ttcagcgtgg
       61 atcgtattac tcctgatgac ggcggatgta gttatatccg gttgcggcgt gaaggagcag
      121 gaaacgtaag ggcaggggga tattatgagg gggcttgaga atgccatccg caatctgaac
      181 agtcttgata cccgtatggt gccgcaggcc agcgcatggg cagtaaaccg ggttgctgca
      241 aaagttgtct cggttgccac ccggcaggtc gccaggaata ccgttgcggg agataaccag
      301 gtgaaggggg gccccctgaa actggtgcgt cagcgcgtcc gggtatttaa agccagtccg
      361 gcaggaaaaa tgtatgcccg tatccgtgtt aaccggggca atctgcccgc cattaagctt
      421 ggcacaacat gccggcgtgc cggtgacgga ctgaaggtgg ggaaatactt ttttcgtggt
      481 gcgtttatcc agcagctggc gaacggtcgc tggcatgttc tcaggcgtct tcctgaagcg
      541 tgttttgaaa caggaaagga cagtaagggg cggcccagaa aaaatcgtct gcccgtggag
      601 gttgtgaaaa ttccgctggc cggaccgctg acacaggcat ttgaggatgc ccgcgatcgc
      661 atcattgccg cggaaatgcc gaaacagctg gggtatgccc tgaaacagca actgaggata
      721 catttgggat gaacaggcac acacaaatcc gccaggcagt actggcaagc ctgaagggcg
      781 aatgcggggg gaatgtcgtg ctttttgacg ggcttccggc atttattgat gcgcaggaac
      841 tgcccgccgt ggcggtgtgg ctgagtgatg cgcagtacac cggaaaaatg acggatgagg
      901 acgactggca ggctgttctg catattgccg ttttcgtccg tgcacaggcg ccggattctg
      961 agctggatac gtggatggag aacaccatat ttccggccat gaaggatatt ccggtgcttt
     1021 ccggactcat cgacaccatg atcccactcg gttttaacta tcaacgtgat aatgagatgg
     1081 ccacctgggc gatggcggaa atcacgtacc agatcacgta cacgaattaa ggaggtggca
     1141 atgaccacac caaatccact ggcaaagacg aaaggtgcgg gaacgacgtt ctggatgtac
     1201 accggcaagg gcgatgcgtt tgcgaaccct ttgtcggaca ctgactggct gcgtcttgcg
     1261 atggtgaagg atctgcaacc tggcgaaatg accgctgatg cagaagatga cacttatctc
     1321 gatgatgaag atgcagactg gaaaacgaca acccaggggc agaaatccgt cggtgatact
     1381 ttggcgacgc tggcctggcg tccgggtgac agcgggcaga aaaaactggt tcagttgttc
     1441 gattccggtg aagtctgcgc gtttcgtatc aaatacccca acggtacggt tgatgttttc
     1501 cgtggctggc tgagttcact gggtaaaacc attacctcaa aagaggtgat gacccgtact
     1561 gtaaaaatca gcggtgtggg gcgtccgtat ctggcagagg agggcgctga aaccgtgggt
     1621 gttaccggac tgacggtggc accggcatct gtcagtgtca aagcgggagc aaccaccaca
     1681 ctgaccttta cagtaaaacc tgacggggcc agtgacaaag cgatcagtgt gcattcgtca
     1741 gatcctcaga ctgcttcggt gaccctgagc gggcttgtgg ccacggtgaa aggcgtgaag
     1801 cagggcagtg tcagcatcgt gggcatgacc tctgacggag agtttgtggc agtggctgcg
     1861 gtgaccgtca gcgcaccata acaggacgat actcatcatt gccccggtta tccggggctt
     1921 tttgcatccg gagaacatga tgtttctgaa acaggatacg tttaattatg aaaaacagtc
     1981 cgtggtgctc agtgagctgt ccgggctgca gagaattgaa tatctgacgt ttgttcagca
     2041 gcgaacggca aagtttgatg cacaggaggg agaactgccg gaggctgaac gacagattgc
     2101 ttttctgcgt atgggaatgg atatcaatgc ctggctggtt tcccgctcac tgtggaatgc
     2161 tgagcagtct caggatgttg agacgctttg cgcatccatt atgacaacat ggtcgtatga
     2221 tgcgctgggc gcgggggcgg agagggttct gtcgctgagc ggtatgggga ccattgagaa
     2281 tgccggggat gatgatcatg aggcgctgac gccggaaaag tcctgacgcg ggaaatgcag
     2341 tttgtcatgc ggcttgcccg ggagttccgg cgggcagact ggcggcggat gctgtcggaa
     2401 atgtcggcca ctgagcttgg tgagggggcg attatttccg gatgcagagc ttcagtgatg
     2461 tgtggatgga tgcgcagttt gcctcgctga aggcattgat cgtgagaatg gtgtccggca
     2521 gcagtgatgc tgcggtggct gatttcagcc ttttaccgga agagaacggg ataccggagc
     2581 gaacggacga agaactgatg catcttgggg aaggtatttc cggaggtgtg cgttatggac
     2641 cagatagcca acctggtcat tgatttgggg attgatgcgg cagagtttaa aaatgaaatt
     2701 ccccgtatca aaaaccttct gaatggtgca gccggtgatg cagaacggtc ttctgcccgt
     2761 atgcagcgtt ttatggagcg tcagactcag gcggcccggc agacaatgca tgcggcttct
     2821 tcggctgcaa cagcagcatc agcccatgcg cagacggtgg agaagaatgc acgggctcat
     2881 gaacgcatgg cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct gaatcagaaa
     2941 atgcgcgagg aacaggcgca ggccagggca ctggcggagg cgcaggataa agcggctgcc
     3001 gccttttatc gccagattga cagtgtgaaa caggccggtg cggggcttca ggaattacag
     3061 cgtattcagc agcagatccg acaggccaga aacagtggcg gggttggtca gcaggattat
     3121 ctggcgctga tttcggagat cacggcgaaa acccgtgccc tgacgcaggc agaggaacag
     3181 gccacccggc agaaagcggc gtttatccgc cagcttaaag agcaggcaac ccgccagaat
     3241 ctgtcgtctt ctgagttgct tcgcgccagg gcggctcagc tgggggtaag cagtgctgca
     3301 gaagtgtata tccgcaaaat ggagcgggca ggaaaagcca cacattcgct ggggctgaaa
     3361 agtgcggcag cccggcggga gctgggggtg ttaatcagtc agatggcgcg cggcaatttt
     3421 ggtgcgctga ggggatccgg gataacgctg gctaaccgtg ccggatggac aggcgcactg
     3481 atgtcgccga aaggcatgat gactggcggc gttattggcg gacttgtcgc ggcggtcctg
     3541 ggtctgggta aagcctggca tgacggccgg aaggagggcg aggaatttaa ccgtcagctg
     3601 gcgctgacgg gacattatgc cggtgtcact gtcgggcagt tgtggaaact cagccgggcc
     3661 atatccggga atggtatcac gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc
     3721 agtggggcat ttcatggaaa cgatattggt atggtggcga aagctgccgc acagatggag
     3781 cgatcggttg gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat
     3841 cctgtaaatg ccgcgaaggc gctggacaat gcgctgcatt ttttgactgc cactcagctt
     3901 gagcagatac gcgtccttgg ggagcagggg cggtccagtg atgcggcccg gatcgccatg
     3961 tctgcgctgg cagaggaaac cggtaaacgc acatctgata ttgataataa tctcaatgcg
     4021 ctgggtagta cgctgcaaac cttgtctgac tggtggaagc agttctggga tgcggccatg
     4081 aatattggtc gtgaagactc gctggatgcg cagattgatg cgttacagga aaaaattcag
     4141 cgcgcgaaaa aatatccgtg gacaaacgcc tccacacagg tggagtacga tcagcagcgt
     4201 cttaacgatc ttcaggagaa aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca
     4261 gaacggaatt accaggagca acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg
     4321 atgaatgaaa cggaagctgc acgacatcag cgggaaattg cgcgtattaa tgccatgcag
     4381 tacgccgacc aggctgtcag ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa
     4441 gccattaaga aaaatacacg ggcaacccgt aatgatgagg ccacccggtt attgctgcag
     4501 tacagtcagc agcaggcaca ggtggaaggg cagattgctg ctgccagaca gtcagcaggc
     4561 attgccactg aaaggatgac tgaagcgcat aaacagcttc tggccctgca gcagcgcatc
     4621 agcgacctgg acgggaaaaa gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat
     4681 gagctgattc aggcgctgac gctgctggat gtgaaacagc aggagctgca gaaacagaca
     4741 gcgcttaacg acctgagaaa aaaaacggtt cagctgacca gccagctggc agacaaagaa
     4801 cgtgcactgc gtgagcagca caatctggat attgccactg caggtatggg ggataagcag
     4861 cggcagcgct accaggcaca gttgcgcatc cggcaggaat accggcaaca gttgcaacag
     4921 cttgagaatg acagtcgcca gaaaggcact tacgggacgg aggactaccg gagggctgag
     4981 gaggtgctga aggggagcct gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa
     5041 ctggaagtgg cgcagggcga ctggaaaaac ggtgccatgc gggcgtttca gaattttacg
     5101 gcggatgcgg ataatgcggt gggaacggca gaacagatgt ttacagtggc attcagcagt
     5161 gccggtaatg cactggcgac attctgtacc accggtaagc tgaattttaa atccttcacc
     5221 tcttccctgt tgtcagatat ggccagaatt atggcacaga tggccatgat gcaggcggta
     5281 aagggcgtcg gctctttatt cggcttcacg actaatgctg atggcggtgt ttaccagtct
     5341 gctgatttga gtcgctacag tggcacggtg gttaaccgtc cgacgttttt tgcttttgca
     5401 aaaggcgcgg gtgtgatggg ggaagcggga cctgaagcca ttctgccact gcgtcgcggt
     5461 gctgatggta agctgggggt tgtggcggat actggtggtt caggcatggt gatgtttgcc
     5521 ccgcagtaca acatcgggat caataacgac ggcacgaacg ggcagatagg tccggcagca
     5581 ctgaaggtgg tttatgacct cgggaaaaaa gcggccgcgg actttatgca acagcaggcc
     5641 cgtgatggtg gccggttaag tggagcatac cggtaatgga gacgtttcgc tggaaggtgc
     5701 gcccggatat gaatgtggta tcagagccaa aagtggtggc agtgaagctg ggtgatggtt
     5761 atgaacagcg tcgcccggcg ggactgaata atctgctgtc gacttacagc gtgacgatac
     5821 gtgttcgtaa aggtgaacac ccacctttaa aagcctttct ggaacggcac ggtggcgtcc
     5881 gcgcatttca gtggacgcca ccttatgact ggaaaccgat cagggtggct tgtcgtaaat
     5941 ggtcggcaag cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa caggtggtga
     6001 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     6061 cagcgtggtg ctctgggaaa tcgacctgac agaggtcggt ggagaacgtt attttttctg
     6121 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     6181 tcccattcag ggggagtggt tttgaactga atggcaaagg caccagtacg cgccccacgc
     6241 tgacggtttc taacctgtac ggtatggtcg ccgggatggc ggaagatctg cagagtctgg
     6301 tcggcggaac ggtggtccgg cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg
     6361 tcaacggaaa cagtgacgcc gatccggagc aggaggtgat cagccgctgg cgcatcgagc
     6421 agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca acggagacgg
     6481 atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg acctaccgtt
     6541 ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa ccaacgacgg
     6601 atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc aacaataccg
     6661 gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat gacacagaca
     6721 gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg tggcttcgtg
     6781 gtgag
//
LOCUS       DAJVTY010000191         6618 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVTY010000191 DAJVTY010000000
VERSION     DAJVTY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.191"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(733..1263)
                     /locus_tag="OQQ66_003668"
                     /pseudo
     CDS             complement(733..1263)
                     /locus_tag="OQQ66_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1606..2256)
                     /gene="zinT"
                     /locus_tag="OQQ66_003669"
     CDS             complement(1606..2256)
                     /gene="zinT"
                     /locus_tag="OQQ66_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1906419.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2513..3148)
                     /gene="msrQ"
                     /locus_tag="OQQ66_003670"
     CDS             complement(2513..3148)
                     /gene="msrQ"
                     /locus_tag="OQQ66_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1906420.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3149..4153)
                     /gene="msrP"
                     /locus_tag="OQQ66_003671"
     CDS             complement(3149..4153)
                     /gene="msrP"
                     /locus_tag="OQQ66_003671"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS1906421.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4262..4618)
                     /gene="hiuH"
                     /locus_tag="OQQ66_003672"
     CDS             complement(4262..4618)
                     /gene="hiuH"
                     /locus_tag="OQQ66_003672"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1906422.1"
                     /translation="MVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTA
                     KTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYH
                     VPLLLSQYGYSTYRGS"
     gene            4789..5460
                     /gene="hprR"
                     /locus_tag="OQQ66_003673"
     CDS             4789..5460
                     /gene="hprR"
                     /locus_tag="OQQ66_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1906423.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5460..>6618
                     /gene="hprS"
                     /locus_tag="OQQ66_003674"
     CDS             5460..>6618
                     /gene="hprS"
                     /locus_tag="OQQ66_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1906424.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDI"
BASE COUNT         1864 a         1352 c         1488 g         1914 t
ORIGIN      
        1 cttctttaat gttatgtaat tcattgattt ttcgtgtacg attttaagac attttttcca
       61 agaaaaattt ttaactcttt gatttttcga ctctgtatca tcggtctcga aaaccggagt
      121 aggggcaact ctaccggggg ggcaaatccc cctctctccg ccactttatc aatgacttat
      181 ctcccgactt cccgccttac ttttcctaaa cagaacaatc gtagaatatt cttgaagggt
      241 tagatcgtca ctgttttctg ttcgatactg tgacattcag cacttgattc gctatggatc
      301 tgacaggaag ctttcgagcg aaaatgtgca gttattcagt cgttttctta tcgctcacca
      361 ttattctttt agacattcat cctatgaaac tgccgcaaag ttggtggtgg gaactgaagt
      421 tgcgtagaga aggggtcaat aattgagtca aaaaaggaac gcggccgtca gattaaagcc
      481 gcgggacaaa gtccataatt tccaagagtt gttgaatttc ccagcttttt tgaaagcctt
      541 tgtatcatac taacaagtaa aaaaggttta aataccaaaa ttaacctgta tttttgataa
      601 atcttaccat tgtacgattt acacagtttc tgctcctcct tgttgatagt ggaaagggtg
      661 cattgtgcac ttgcgtaaac atttatgacg cgttgatgtc catggttcac atttccttgt
      721 caggggatcg acttatttgc ggcgaggcat cattcgtaac agagtattat ctttctgaat
      781 gtagtgatga aagagtgctg cgccagcgtg agctgcgatt aaaaagtaac ctatatttgc
      841 ccaggtttcg tgaatatttt atatcagtgc tttaatttcg ctgtttgggg taacaaaggg
      901 agacacattg aaaccaagga aactccacga ttttccactg taagccatca aagcaatccc
      961 cagcagagga agtgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt
     1021 catttgccag gcaggtggcg gtggcacaat agaaggatca ctataacggt gttttataat
     1081 caggcgtgaa aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat
     1141 cagcagataa gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt
     1201 tattgcaacg aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt
     1261 catttttgta gctcctgtaa tttttacagt caagctatcc cacgaacctt aaaaattcct
     1321 tatacagatg tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca
     1381 gccaatataa gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg
     1441 aatccgttat tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg
     1501 atgagcgtgt ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt
     1561 atgatgactt actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat
     1621 ttcctcgacc acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat
     1681 ttcatttaat aaagattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt
     1741 tcgtggtgca attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc
     1801 tttacattcg aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta
     1861 tccatcgtaa tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc
     1921 gtcctcaatg ccaatcatct cgatatctgt tgcataacct ttgtgataat aatctttaat
     1981 ttcagcaaat gttttagttt tatctgcatc cgctttcttc tgaaagacgg ggtcaagttt
     2041 gccactttgc agtaaaggat aaacggattg ccagacccca tcccagtcac tgagcgttcg
     2101 gttttgtaca ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa
     2161 gggtttgccg tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc
     2221 taacgcaaca gccagtttgt gaagacgaat cgccaaaaca gtttcctcca gagtcattgt
     2281 atatgatgtg taatgttata ttgtaacata tggcaaaatg caacaactct taggagatga
     2341 gagcatagtt ttctcaggtg tacttatcta tcttattgaa ataaaatgaa ttatattgat
     2401 aatttgaggt tgatgacaaa cgaaacataa tctgatgtga tctgcgtagg ccggataggg
     2461 cgctcgcgcc gcatccggca gggataacgc gtactttgtc ggcaatatga agttatacag
     2521 ataatttgtt gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcaac ttcttatacc
     2581 gtaaggctaa aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct
     2641 tcactgacca cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca
     2701 actgttgcca gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag
     2761 caagcaaaat aacccagctg ataattccta acgttaaata aggccgggta attaactcct
     2821 tgcctaataa cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca
     2881 gtgtcgccca ggcaaagcac cataagccta acaggcggcg agtgcgtatc aataacggct
     2941 gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag
     3001 tgcgaccggt aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga
     3061 tcgcccagac cagccagaga aacggcaaca atccggcaag atgcaggcta actttcagcc
     3121 atgtcacctg ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat
     3181 acagcgatgc cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgaacat
     3241 cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa
     3301 catgcggatt aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg
     3361 ttggcggacg ctcgcgggtc agcttaatac tgactatcga tttaatccct ttaaagccat
     3421 atttccacgg cacaatcagt cgtaccggcg cgccattttg tggcggtaac gccttgccat
     3481 aaacacccac ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga
     3541 cataaggata tttcagcccg ccgccgataa agcggtcctg ctggcctggc atttgttccg
     3601 gtgcataaat tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca
     3661 gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat cgaccatgcc tccacgcagc
     3721 gcatacgata aatacgctct tccagcggga agcgacgggt taaatcatcg tgatcgaggg
     3781 tcaatggttt tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac
     3841 cagcattagc ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg
     3901 agactttatc tactggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact
     3961 ccagcggttt tccggcgggg gcgggcgggc gatcgttccc tttaaaccag ctaagcagat
     4021 cggcatgcgc agcgtgaggc aaagaaagtg cagctgcact gatgcccagt gcttttaaca
     4081 cctgccgacg cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt
     4141 gattcttttt cataagccat cacacttttt tgtcgttagc cagcaagagc cgtttactgt
     4201 taatctgtca gggagtgtcc tccctaacag tagcagagat ctgcttttac atatttatgc
     4261 attaactgcc acgataggtt gaatacccat attggctaag taataaaggc acatgataat
     4321 gctcgttcac tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat
     4381 tttgtttctt gaaatagtcc ccggttttaa atacgacacg gtaatcgccc gtagttgcag
     4441 tttgctcggg ccacagtgct ttaattcgtc catccttatc tgttttggcg gtattaagtt
     4501 gtaaccagcc gttatccgcc ttcttttcaa gagtgactgt cacgtcagca gcgggttttc
     4561 cggtttgctg gttcaaaatg tgcacgctaa gaatgttttg ttgtgcggca taaaccaaag
     4621 aaggtaatga aaatgctgca aataacgctt taacatgttt atatccttgt catgtgaatg
     4681 agttttgagg atagcgaaaa agcggccagg gtaaccttac agcagcatta caattttgta
     4741 atgaaccagg ctgtttctat aacatatgat ttatggcata ttattttcat gaagatttta
     4801 cttattgaag ataatcaaag gacccaggag tgggtaacgc aggggctttc cgaagcgggt
     4861 tatgtcattg atgccgtttc tgatggcaga gatgggcttt atcttgcgct gaaggatgat
     4921 tatgcattga taattctgga tattatgctt ccgggtatgg atggctggca gatcttacaa
     4981 acgttaagaa cagcaaagca aacccctgtt atttgcctta ctgcaaggga ttctgtcgat
     5041 gacagagtca gagggctgga cagtggagca aatgattatc tggtaaaacc tttttcattt
     5101 tctgagttgc tggcaagggt tcgggcacaa ttaaggcaac atcacgcttt gaattcaaca
     5161 ttagaaatca gtggcttaag aatggactct gtaagtcaaa gtgtgagcag ggacaatatc
     5221 agtattacac tgacgcgcaa ggagtttcag ttactttggc tactggcctc cagagctggc
     5281 gaaattatac ccagaacggt tattgcgagt gaaatttggg gaatcaactt tgatagtgat
     5341 accaatacgg tggatgtcgc cattcgcagg ctccgcgcaa aagttgatga tccttttcct
     5401 gaaaagctaa tcgccacaat ccgggggatg ggctattcat tcgtagcggt aaaaaaataa
     5461 tgaaaagatt gtctataacc gtccgtttaa ccttgctttt tatattgctg ctgtctgttg
     5521 ctggcgctgg aattgtctgg actctctata atggcctggc aagtgagttg aaatggcgcg
     5581 atgatacaac actcattaac cggacagcac agatcaagca gttgttaatt gatggggtaa
     5641 atccagatac gttacctgta tactttaacc ggatgatgga tgttagtcag gatatcttga
     5701 tcattcatgg tgatggcatc aataaaattg ttaaccggac aaatgtcagt gatgacatgt
     5761 taaataacat acctgctagt gagacaatca gcgcagctgg catttacaga agcattatta
     5821 atgatactga gattgatgct ttacgaatta atattgatga agtttcgcca tcattaacgg
     5881 ttactgtggc taaattggct tcagctagac ataacatgct tgaacagtat aaaattaata
     5941 gcattataat ttgcattgtc gccattgtac tttgctcagt attaagtccg ctgttaatca
     6001 gaacgggatt acgagagatc aaaaagttga gtggtgtaac ggaagcgctg aattataacg
     6061 atagccgaga gcctgttgag gttagcgcat taccgagaga actaaaacct cttgggcagg
     6121 cgttgaataa aatgcatcaa gccttagtca aagattttga acgcctaagt caatttgctg
     6181 acgatctcgc tcatgaactt agaacgccca ttaatgcatt actgggtcag aatcaggtta
     6241 cgctcagtca aaccagaagt atcgctgaat atcaaaaaac aattgccggt aacattgaag
     6301 agttggaaaa tatttcgcgg ttaacagaga acatactgtt tcttgcccgg gcagataaaa
     6361 acaatgtttt ggtgaaactg gactcgcttt ctctcaataa ggaagtcgaa aatttgttgg
     6421 attatcttga atatctttca gacgagaaag agatttgctt taaggtcgag tgcaatcagc
     6481 aaatctttgc ggataaaatt ttactgcaac gaatgttatc gaatcttatt gttaatgcca
     6541 ttagatattc accagaaaaa tcgcgtattc atataaccag ttttcttgat accaacggct
     6601 atcttaatat tgatatcg
//
LOCUS       DAJVTY010000192         6460 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVTY010000192 DAJVTY010000000
VERSION     DAJVTY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.192"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..526
                     /locus_tag="OQQ66_003675"
     CDS             <1..526
                     /locus_tag="OQQ66_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1906425.1"
                     /translation="LPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDV
                     LLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHREWHASAF
                     TGICNDLAAASTFV"
     gene            463..1065
                     /locus_tag="OQQ66_003676"
     CDS             463..1065
                     /locus_tag="OQQ66_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1906426.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1126..4605
                     /locus_tag="OQQ66_003677"
     CDS             1126..4605
                     /locus_tag="OQQ66_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1906427.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGVESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            4673..5272
                     /locus_tag="OQQ66_003678"
     CDS             4673..5272
                     /locus_tag="OQQ66_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1906428.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5336..>6460
                     /locus_tag="OQQ66_003679"
     CDS             5336..>6460
                     /locus_tag="OQQ66_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1906429.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NGPIYHPRYCYWPISRLTDWVKINITTEDIIYRIVASSVRN"
BASE COUNT         1495 a         1594 c         2016 g         1355 t
ORIGIN      
        1 tctgccctgg ctgagtgagg ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg
       61 gctggtctgc cggggggcga ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcttatc atctggcggg
      181 gattgagatg ccggattttc atcgcgggga tgactggtgg cgtcacggtc agaatctcta
      241 tctggataat ctggaggcca cagggctgta tcaggtgccg ttgtcatcag cacaaccggg
      301 cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgcgg
      361 cgacggtgag ctgctgcacc atattcctga acaactgagt aaacgagaga ggtataccga
      421 caaatggcag cgacgcacac actccctctg gcgtcaccgg gaatggcacg catctgcctt
      481 tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa
      541 gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag tgacggctgg
      601 tatcaggtgc gcattgccgg gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat
      661 gagccgctgg caaatggtgc agtgatccac atagtaccgc gtctggtggg agctaaaagt
      721 ggcggtgtgt ttcaggcggt gctgggggca gctgttatgg cggttgctat atggatgccg
      781 ggggtaggaa ttatggcgag taatctgctg ttttctctcg gtgccagtat gacgcttggc
      841 ggtgttgcac agatgctggc accgaaagcc agaactcccc gtacacagac aacggataac
      901 ggcaaacaga acacctgttt ctcctcactg gataacatgg ttgcccaggg caatgttctg
      961 cctgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tatctcagga gatcagcacg
     1021 gcagacgaag gggatggtgg tcaggttgtg gtgattggtc gctgatgcaa aatgtttcat
     1081 gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc
     1141 agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca gttgctgagt
     1201 gtgattgatg ccatcagcga agggccggtt gacggtccgg tggatggatt aaaaagcgtg
     1261 ctgctgaacg gtacgccagt cctggacagc gaggggaaga caaacttttc cggtgttacg
     1321 gtggtgttcc gcgccggcga gcaggagcag acaccgccgg agggggttga atcttccggc
     1381 tcagagactg tgctgggtac ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg
     1441 tcggcaaaca ttgatcgtct gcgcctgacc ttcggtgtgc aggcactggt ggaaaacacc
     1501 tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt
     1561 ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacttcgca gtatctggcc
     1621 tcggtggtgg tgggtaacct gccgccgcgc ccgtttaata tccggatgcg caggatgaca
     1681 ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcgta caccgaaatc
     1741 atcgatgtga aacagtgcta cccgaacacg gcgctggtcg gcgtgcaggt ggattcagag
     1801 cagttcggta accagcaggt gagtcgcaat tatcatcttc gcgggcgcat tctgcaggtg
     1861 ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg aacgcttaag
     1921 ccagcataca gcaacaacat ggcctggtgt cggtgggaca tgctcactca tccgcgctac
     1981 ggcatgggga aacgtcttgg tgcggcagat gtggacaaat gggcgctgta tgtcatcggc
     2041 cagtactgcg accagtcagt gccggacgga tttggcggca cggagccgcg catcacctgt
     2101 aacgcttacc tgaccacaca gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg
     2161 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg
     2221 gataaagtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc
     2281 cgctacagct tcagcgccct gaaggaccgc cataatgtcg ttgaggtgaa ctggattgat
     2341 ccggacaacg gtcatgagac ggcgacagag cttgtggagg acgcgcaggc cattctccgt
     2401 tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg acaggcgcac
     2461 cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc
     2521 gtcggcgcag aagggcttcg ccatgtaccg ggtgatgtca ttgaaatctg cgatgatgat
     2581 tatgccggta tcagcaccgg tggtcgcgta ctggcggtga acagccagac ccggacgctg
     2641 acgctcgacc gtgaaatcac gctgccgtcc tcctgtacca cgctgataag cctggttgac
     2701 ggaaatggca atccggtcag cgtggaggtt cagtccgtca ccgacggcgt gaaggtgaaa
     2761 gtgagccggg ttcctgacgg cgttgcagaa tacagcgtgt gggggctgaa gctgccgacg
     2821 ctgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc
     2881 atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg ggcacacttt
     2941 gacggtgacc ggcgcggcac ggtgaatggt gtcacgccgc cagcggtgca gcatctgacc
     3001 gccgaagtca ccgcagacag cggggaatat caggtgctgg cgcgatggga cacgccgaag
     3061 gtggtgaagg gtgtgagctt cctgcttcgc ctgaccgtgg cagcggacga cggcagtgag
     3121 cggctggtca gcacagccag gacgacggaa accacatacc gcttcaggca gctggcgctg
     3181 gggaattaca gtctgacagt ccgggcggta aatgcccggg ggcagcaggg cgatccggcg
     3241 tcggtatcgt tccggattgc ggcaccggca gcgcctgtca ctattgaact gataccgggg
     3301 tattttcaga taacggcggt cccgaaactg gctgtatacg acccgacggt gcagtttgag
     3361 ttctggtttt cggaaaagcg gattgcggat atcaggcagg ttgaaaccag cgcccgctat
     3421 cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaggccggg ccatgattat
     3481 tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg catttgtgga ggccgtcggt
     3541 cgggcgagcg atgatgcgga aggttatctg agtttttata aagggttgat caataacacg
     3601 catctcggca aggagttgtg gacgcagatt gataacggtc agcttgcgcc ggacctgact
     3661 gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcaaaccgt caataaaaaa
     3721 ctggaaaatc agagtgctgc aatccagcag atacagaaag ttcaggttga tacaaataat
     3781 aacctgaaca gcatgtgggc tgtgaagctg caacagatga aggacggacg cctttatatt
     3841 gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca ggtgctgctg
     3901 gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc gatgtttgtt
     3961 ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac ggctccgacc
     4021 attaccagcg gcggtaatcc tccggcattt tccctgacac ctggcggacg gctgacggcg
     4081 aaaaatgccg atatcagcgg taacgtgaac gcgaactccg ggacgctcaa caacgtcacg
     4141 attaataaga actgtcgggt tctgggaaaa ttgtccgcga accagattga aggcgatctc
     4201 gttaaaacag tgggcaaacc tttcccacgg gactcccggg caccggagag gtggccatca
     4261 gggaccatta ccgtcagggt ttatgacgat cagccgtttg atcggcaaat tgttattccc
     4321 gcggtggcgt ttcgcggtgc taaacatgag cggaagaata acaatattta ttcgtcatgc
     4381 agcctgatag tgaagaaaaa cggtgctgaa atttataacc gaacgaccct ggataatacg
     4441 ctgatatata cgggtgttat tgatatgcct gccggtcacg gtcacatgac gctggagttt
     4501 tctgtatcgg catggctggt aaatggctgg tatcccacag caagtatcag cgatttgctg
     4561 gttgttgtga tgaagaaagc cactgcaggc atcacgatta gctgaatttt ataacccaga
     4621 tacgggcacc agaaatggtg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa
     4681 agtttgtgca gccattttgt ccgcagccat ctgtctggct gtatccggtg cgcctgcatg
     4741 ggcgtctgaa catcagtcca cgctgagcgc cgggtatctt cagccccata ctgatatgcc
     4801 aggcagcgat gacctgaagg gcattaacgt gaaataccgt tatgagttta cggacacgtt
     4861 ggggctggtg acgtcattca gctatgccaa cgctgaagat gagcaaaaaa cgcattacag
     4921 cgatacccgc tggcatgagg attccgtgcg taaccgctgg ttcagcgtga tggcggggcc
     4981 gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggcgtgg cttacagccg
     5041 tgtgtcgacc ttctccgggg attatctccg cgtaactgac aacaagggga aaacgcacga
     5101 tgtgctgacc gggagtgatg acaaccgcca cagcaacacg tctctggcgt ggggggctgg
     5161 cgtgcagttt aacccgaccg aatccgtggc cattgacctt gcttatgaag gttccggcag
     5221 tggcgactgg cgcactgacg gtttcatcgt gggtgtcggt tataagttct gattagccag
     5281 gtaacacagt gttatgacag cccgctggtt caggcgggct ttttgtgggg tgaatatggc
     5341 agtaaagatt tcaggtgtac tgaaagatgg ggcgggtaaa cctgtcgtaa attgtgcgat
     5401 tgaactgcgg gccagaagaa ccagtccgac cgttgtggca cacgttgttg ccacttgcgt
     5461 gacggacaat aacggtgctt atgtgattga ggctgagccg gggtattacg aggttgcgct
     5521 tcactgtaac ggctggcagc caacccgtgt cggggatatt gatgtggcac cgactgatgc
     5581 accggggaca ctgaacgcgt ttctgaatgc accaaaggat ggtgatttac gtccggaggt
     5641 gatgaagcgc tttgaggaaa tggtggtgca ggcgcagcag agcgccgggg ccgcagccgg
     5701 aaacgcacag cagacggcgc aggatgtggc ggcagccgca acggcccgtg atgatgcaca
     5761 acgttttgcg gagaatgcca gacaggatgc aaccgtcaca gctgaggaca gaaaggccac
     5821 tgcggaagat gtgacaagca caggagcaaa tgcagccgca gccggacaga gcgcacagga
     5881 tgccgcaggt tatgcccgcg cagcagaaca ggccaaaaat gacattgatg ctgcgctgac
     5941 cggcactctg aaaatggcta accatctgtc agaaatcgca gcagcaggcg aaaaggcaca
     6001 acagaagtcc cgggataatc tggggctgaa aagtgcggcc acgatggaag cacagagcga
     6061 catttacgac cggacaaaag gccgtctggc gatacccggc gcattcggct ttgggtgtgc
     6121 ttttctgcct gaagatgtta tccgttttga cactaagagt gatttcctgg cctgggtaag
     6181 gaatgcgctg ccaggtgaat attccgttgc tggcccctac gacatcatca tacccgacac
     6241 acggtttgaa ggggtgctca gcatccggtg gactgatgca cgccctgaga caacagaacc
     6301 gcggtacaga gccaaatccc ttacttttta cggcattaac ggccccattt atcacccccg
     6361 ctactgctac tggcccatat ccagactgac tgactgggtg aaaataaata taaccacaga
     6421 agatattatt tacagaatcg tggcgagctc tgtccgcaac
//
LOCUS       DAJVTY010000193         6450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVTY010000193 DAJVTY010000000
VERSION     DAJVTY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.193"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            265..471
                     /locus_tag="OQQ66_003680"
     CDS             265..471
                     /locus_tag="OQQ66_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906430.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            471..1526
                     /locus_tag="OQQ66_003681"
     CDS             471..1526
                     /locus_tag="OQQ66_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1906431.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            1538..1873
                     /locus_tag="OQQ66_003682"
     CDS             1538..1873
                     /locus_tag="OQQ66_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1906432.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            1886..2299
                     /locus_tag="OQQ66_003683"
     CDS             1886..2299
                     /locus_tag="OQQ66_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1906433.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            2461..2895
                     /locus_tag="OQQ66_003684"
     CDS             2461..2895
                     /locus_tag="OQQ66_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906434.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            3176..3457
                     /locus_tag="OQQ66_003685"
     CDS             3176..3457
                     /locus_tag="OQQ66_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906435.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(4135..>6450)
                     /gene="yejO"
                     /locus_tag="OQQ66_003686"
     CDS             complement(4135..>6450)
                     /gene="yejO"
                     /locus_tag="OQQ66_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1906436.1"
                     /translation="YSVLNDGYQIVQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLI
                     VEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVMEAFGINRLGQFEIK
                     NGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTN
                     ALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTG
                     TKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGTMVNVSVRGNDGILE
                     VMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSVWAIIADITTTNQNT
                     LLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGNGNFYMRTDMANHQS
                     DQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEY
                     TLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTVRERLGSVKSVSYDT
                     AMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGF
                     DRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNG
                     AGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAV
                     SYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAGTPGVYQAGIRSSFT
                     PTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         1731 a         1594 c         1519 g         1606 t
ORIGIN      
        1 gatcagagac tgatccagca cacagcacac atagccggag caatccggct tttttgcgcc
       61 caaaaaaagc ccgataaggt cagagggttc ttatcgggct tttgcatatg aggtttttgg
      121 atgcactggc gttcgtgatc gggataatca tttcataatt tgcaacataa ctcaatatta
      181 ttgcataaaa tgcaattctg attataatca ggactggata aacatccagt cctgattttt
      241 ttagtcgaag aggaatttct tactatggct gaagagaaaa aaggcggtgt ttcggtgtac
      301 ataagccccg aaatcgtgga ggtgctcaag cagcgccaca aaaaaaacta tgaggctggc
      361 gtggcggctg gactggatcc gctgatgacg ccggagccgt cgataggttc acttgtacgc
      421 tcttatttac ttgcggcgct tgggatgcat aaaaattatg ggggtgaata atggcaggca
      481 aagcaacggc acttaacact aaccagcttt ttatgtacct gaatcgcggg gatattgcgg
      541 attttaaatt cagccctctg tttaccacgc tgtttttccc gaacgtggcg acattcagca
      601 cgcaaaacat catgctggat accctggaca ttgaagaagt caccatgtcg gcgttttgtt
      661 cgcctatggt gggtagccag gttcagcgcg ataaagggta cgaaaccagc acaatcaaac
      721 ctggctacat gaagccaaag cacgaaatcg atccaacgaa aaccatcatg cgcatggctg
      781 gagaagatcc ggcacagctt aacgacccta cctatcgccg tatgcgcctg attactggca
      841 acatgcgccg ccagataaac gccattaagg cacgcgtgga atggctggcg gtgaatgcgg
      901 taacgaccgg aaaaaacatc attgagggcg aaggcataga acgctatgaa atcgactgga
      961 agataccgga aaaaaacatc atagagcagt ccgacggcaa aaaatggtcc gagcatgata
     1021 aagatattta cgatccaatc tatgacatcg aactatacgc agatcaggca ggttgccccg
     1081 ccaacgtcat gattatgggc gctgaggtat ggcgcacgtt acgcagcttt aaaaaattcc
     1141 gtgaactgta cgatctttcc cgtggttcag aatccgccgc agaaatggcc tgtaaaaacc
     1201 tgggcgaagt ggtgagcttt aaaggctatc tgggcgatat tgctcttatt gtctattccg
     1261 gcaaatacac tgacagcgac ggcaccgaaa aatatttcct tgagcctgat ttgctggtcc
     1321 tgggcaacac caacaataaa gggctggtgg cctatggtgc gattatggat caggaagcgg
     1381 taagaacggg cgcaacgcaa aacatgtact acccgaaaaa ctggattgag gacggcgatc
     1441 cggcgattga gtacgtgcag acacacagcg caccgcagcc ggttccggca gatattcgca
     1501 aatttgttac cgtcaaaatt ggttaacggg ggattatatg gacactccat atattgagtt
     1561 atttgcaggc agtcagcagg tatcaacgac actggtacat tttgccgctg atgctggcgt
     1621 tattcaggaa tttaccccgc tgatgctggt ggacaatggc gagtttaagc cgtgggatgg
     1681 tcaggaatct ggcaaggctg tttatctgac ttcgtacctc gtggacacgt caaagcagaa
     1741 atcagcacag tgttacaaga cggggatatt caatattgcc gccgttaact ggcctgagag
     1801 cgtcgacacc gatgcgaaaa aatgcgccgc ctttgcgggt tctggcgtat ccgttcagcc
     1861 gctggcccga taagcagggg gaacgatggc aacgaatgaa agcatcatgg cgctaccgct
     1921 ggcgagtaaa tttaaagccg aagcgcgggc aatggctgac agaggtttat caacctacga
     1981 ggccgtatat caactcaaca aactggaaga gcaggacaag ccgcgcgctg atgcgattat
     2041 ggcgcttcat gagcataaag actatcagcc gctgttgcgt gcaatggcaa acgtgccttg
     2101 tatcgatgtt gatacggcta aaaacatcct gaacatgacc atagagcagg aacgcccgaa
     2161 ggttgcacca gagcttaccg cagcctttga aaactttatg gacatgcaca gcccacaaac
     2221 cgtatcagct ggcatggcat acgatggcag aaacccaggc gatgacggcg acatcgatcg
     2281 catactgaaa accatctgag acaaggccgg agaaatccgg cttttttgcg ggtccttcct
     2341 ggaattatgg cccgttacgg ggcggcgacc tcgcgggttt tcgctattta tgacgttttt
     2401 ccgtgaaggt gacaccacca ccacttgatt aatatttaac catgcagtta aggtaacatt
     2461 atgattgata aagcttgttt tgtaagtcag caggaaatag ctgaacattt caaggttaac
     2521 agaaccacta ttcgcgcatg gaccaaacag gggatgccgt atcttaatgc ggatcgcgga
     2581 aagtctggcg gttatcacat cgggcataca ttgctttggt cttcaggtaa aagccgtctt
     2641 gaggccatca gatatcacgt agaaaccagt gcgctggaaa aaattatgtt tgccaggctg
     2701 ctttcatctg agcgtgacga gtactccagt gaagaaacag aacatcgatt tgatgaaggt
     2761 ttgcagattt acggctattc accggaagat gtgagcaagg cacgaaataa aatggctggc
     2821 tttctggctg ggtggcgtca tgccgtaagc gttcgccgtg ccagcatgga gcaatcagcc
     2881 gatacagaac agtaaagccg ataacttgtt aatcactcgc agcaatgcat gtgatttgtg
     2941 cacactttcg gttaactggt tcgatgtccg gttttagtgt ctgttttttg cgcatgtccg
     3001 gttcatggaa agcatgtttt tatatttttc atatggttaa cttgtagaga aaccggacat
     3061 ggatcccgaa aaattttcat aaatagtgaa aacgcgcgag gtcgccgccc cgtaacgggt
     3121 ccataattcc aggaaggacc cgacgacacc agacaatcag aacgataggt gcacaatgac
     3181 agaagccgaa atactgggat taatccgccg tgctggtgga atcagccagc agactgacga
     3241 acaggccacg cagccggaca gcgtgacagc cgaaaattat gcgcgtgttg ttgctgaggt
     3301 gatgcgccgt gatggtatcc agcttaatga tgtggatatg cgcaacatac ggatccgcgt
     3361 tcttgaaatg ctggcctaca atcgccgcgt ggcactgtat cgggaaacgg agaaaattac
     3421 ataccactgg aagaagccgg agcggttgcg gcggtaactg gttgatattt tcgaaattcg
     3481 ccagcggcga acatcgggga aaccagccta acccgttgat attttcggga acctcaaatt
     3541 gaggaggtcg gaaacataac ccgtcatatt ctggatgctg ataattacca cgcagcccat
     3601 taaggcctaa cccgttgatt ctttcggaaa cagccattgg cgtaatccgg cgcggtaact
     3661 tgctgaactt aaagcaaagc gcaaaattgc gctggctggt gggcatgatg ccgatatggt
     3721 gatccccata acggaaatat ccaccagcac ggcaacggca gaatagccac aacacagaaa
     3781 aaccacgaat atggggtttt tgttatggca tgggcatgat gaccgcccct gctctgaatg
     3841 gtggaaatca ccacgcagat catccaccag cttcgttttt ataaattaaa catccgggaa
     3901 aatccgaccc tgaattgtgc attttttcag caaatcattt tagttatacg tttggttata
     3961 cggtttaaaa gttgaatcat atacattatt ttaaatcatg aagttacacg ctcaattaaa
     4021 ctactctcgt gaaaaatccc aagaaaaaac caacctttac ggttggtttt tttatatctg
     4081 caattaattt gataaacaga ccgtgacaca tcacagcctg tttattttct gttatcagaa
     4141 cgtccagacc actcccgcct gagtattcca cggcgattct acccctgcgc cattgccata
     4201 gctgactgac aaatgaccgc ttaacgtcgg ggtaaacgat gaccttatcc cagcctgata
     4261 aacgccaggg gttccagcca catcattatt aaatttgcca tcgtcattaa ctttcacatg
     4321 gttagaatcg gcgtattcct gacgtacggc ggctttcagc cagggttcca gcgtcgtacc
     4381 attttgcagg tccatgtgat agcttaccgc cgttcccgct tcagcgcgta atatccgggt
     4441 atttcccaca tcagcgcgca tgccgtttga taacgtgtag tcctgaccat ctgtggtaaa
     4501 gccggtaaag gccagatagg gtctaacact ccacaatccg tcaacccaac ggaacccgct
     4561 ctcaacatga gcacccgcgc cgttactatt gtaatcgcca aacgctgttg ccccattact
     4621 catcttgcca tggatggtgt tggcaaaacg gtcaactttc accaccccat caacataggc
     4681 accgttctga tgctcccaac cggcataagc ccccagggta tagctatcga tattaccttt
     4741 tccgccgcga tcaaaaccaa tatcagaatg agagtaacca aagaacaagc cgcgaattgt
     4801 actgctttct tcacgggaga aacggctatc gataccgagc gtcaggcccg tcaatgtttg
     4861 ctcaaaacca gctcccgcat cagtggtcac gttgttgcgg gtgttaattg ccgaactcca
     4921 catcgccgta tcgtaactaa cgctttttac gctaccaaga cgctcacgca cggtgtccag
     4981 ttctgcatca aataccagcg gttgtgcggc cgccatattc agcacatcag tggttgaagg
     5041 ggtaatttgc gcgcgattct ctgccaaact ccagctatgg ttgccattat ccagcaaggt
     5101 atattcatac gtaccgatat caacaacgcc tccggcattg cccaacgtaa atgcagcatc
     5161 accgccgccc gttgttacca gtgtaaggct atctcctgct gccgggctgg caccggtgtc
     5221 cgtcacgaat attttgaaat cacctgttgc ctgaccggtg acgttgagct gatcgctctg
     5281 atgattagcc atatcggtac gcatataaaa attgccgttt cccgacaggg tattggtggt
     5341 caacgtaatg aaattttccg cacttgccgt cactgatgaa cgagtcactg gctcatccat
     5401 cataatcaca tttgcgtcag acatcgcaag gttggctaag ttgaggaggg tgttttggtt
     5461 cgtcgtagtg atatcggcaa tgatggccca tacgctattt tcgagcgaaa cgtccgcttt
     5521 gctggtatcc acggcaccat gcgttctaaa agaagcgccc tcagaaacca ctaccttacc
     5581 caccaacata gcgggtgcat aatttatttg cggcttcatg acctcaagaa tgccatcatt
     5641 ccctctgact gacacgttaa ccattgtgcc agcccagaca ttagcgcggc cattgttgat
     5701 aaccatattt tcagagatgg ctttagagga atacgtgata cttccattct ggtctgatcg
     5761 accgagatca tataccgcat ttgcctgcac tttcgtacca gtatcctttc ccagcgattg
     5821 catagtggca tgctcatcaa ggatagtatc aatggcctgc gtgtcctcca taacaatgag
     5881 cccggaacca tcatccagtt cataattttt tgacacacca tcttttatac taaattggcc
     5941 tttactgttt gtaccactca cttccagcgc attcgttgag acaattaatt ttccgcctgc
     6001 ttttttatca acgccagttg cagtcccgcc atccataacc tccagtaagc cgccactatc
     6061 tacagtagta ttataagcga agtcattttc ttcaactcgc aaacttccgc cgttttccaa
     6121 cagcatattg ttagcaatac cattcttgat ttcgaactga ccgagacggt ttattccgaa
     6181 tgcctccatg acccgcgtgc ttgctttaat agcaccgcct gctttctgat ctaccgcaat
     6241 cgctaatccc ccctctttaa cctccagtaa tcctcccttt tcgatagcgg caaggacggt
     6301 aatcccatct ttttcaacga ttaagcgccc gcctttaatc gtgggatcat tcgctgcgcc
     6361 ataaacctgt agcacaccat tattgagggt agtctggttt gccgcgccac ccatttgaac
     6421 aatttgataa ccgtcattta atactgagta
//
LOCUS       DAJVTY010000194         6450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVTY010000194 DAJVTY010000000
VERSION     DAJVTY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.194"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            188..2104
                     /gene="dnaK"
                     /locus_tag="OQQ66_003687"
     CDS             188..2104
                     /gene="dnaK"
                     /locus_tag="OQQ66_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1906437.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2193..3323
                     /gene="dnaJ"
                     /locus_tag="OQQ66_003688"
     CDS             2193..3323
                     /gene="dnaJ"
                     /locus_tag="OQQ66_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1906438.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3427..3579)
                     /gene="mokC"
                     /locus_tag="OQQ66_003689"
     CDS             complement(3427..3579)
                     /gene="mokC"
                     /locus_tag="OQQ66_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1906439.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4165..5331
                     /gene="nhaA"
                     /locus_tag="OQQ66_003690"
     CDS             4165..5331
                     /gene="nhaA"
                     /locus_tag="OQQ66_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1906440.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5397..6296
                     /gene="nhaR"
                     /locus_tag="OQQ66_003691"
     CDS             5397..6296
                     /gene="nhaR"
                     /locus_tag="OQQ66_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1906441.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6334..>6450)
                     /locus_tag="OQQ66_003692"
     CDS             complement(6334..>6450)
                     /locus_tag="OQQ66_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1906442.1"
                     /translation="HPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1630 a         1530 c         1700 g         1590 t
ORIGIN      
        1 ttctgcgcaa aagcacaaaa aatttttgca tctccccctt gatgacgtgg gttacgaccc
       61 catttagtag tcaaccgcag tgagtgagtc tgcaaaaaaa tgaaattggg cagttgaaac
      121 cagacatttc gcccctatta cagactcaca accacatgat gaccgaatat atagtggaga
      181 cgtttagatg ggtaaaataa ttggtatcga cctgggtact accaactctt gtgtagcgat
      241 tatggatggc accactcctc gcgtgctgga gaacgccgaa ggcgatcgca ccacgccttc
      301 tattattgcc tatacccagg atggtgaaac tctggttggt cagccggcta aacgtcaggc
      361 agtgacgaac ccgcaaaaca ccctgtttgc gattaaacgc ctgattggcc gccgcttcca
      421 ggacgaagaa gtacagcgtg atgtttccat catgccgttc aaaattattg ctgctgataa
      481 cggcgacgca tgggtcgaag ttaaaggcca gaaaatggca ccgccgcaga tttctgctga
      541 agtgctgaaa aaaatgaaga aaaccgctga agattacttg ggtgaaccgg taactgaagc
      601 tgttatcacc gtaccggcat actttaacga tgctcagcgt caggcaacca aagacgcagg
      661 ccgtatcgct ggtctggaag taaaacgtat catcaacgaa ccgaccgcag ctgcgctggc
      721 ttacggtctg gacaaaggta ctggcaaccg tactatcgcg gtttatgacc tggggggtgg
      781 tactttcgat atttctatta tcgaaatcga cgaagttgat ggcgaaaaaa ccttcgaagt
      841 tctggctacc aacggtgata cccacctggg tggtgaagac ttcgacagcc gtctgatcaa
      901 ctacctggtt gaagaattca agaaagatca gggcattgac ctgcgcaacg atccgctggc
      961 aatgcagcgc ctgaaagaag cggcagaaaa agcgaaaatc gaactgtctt ccgctcagca
     1021 gaccgacgtt aacctgccgt acatcactgc agatgcgacc ggtccgaaac acatgaacat
     1081 caaagtgact cgtgcgaaac tggaaagctt ggttgaagat ctggtaaacc gttccattga
     1141 gccgctgaaa gttgcactgc aggacgctgg cctgtccgta tctgatatcg acgacgttat
     1201 cctcgttggt ggtcagactc gtatgccaat ggttcagaag aaagttgctg agttctttgg
     1261 taaagagccg cgtaaagacg ttaacccgga cgaagctgta gcaatcggtg ctgctgttca
     1321 gggtggtgtt ctgactggtg acgtgaaaga cgtactgctg ctggacgtta ccccgctgtc
     1381 tctgggtatc gaaaccatgg gcggtgtgat gacgacgctg atcgcgaaaa acaccactat
     1441 cccgaccaag cacagccagg tgttctctac cgctgaagac aaccagtctg cggtaaccat
     1501 ccatgtgctg cagggtgaac gtaaacgtgc ggctgataac aaatctctgg gtcagttcaa
     1561 cctggatggt atcaacccgg caccgcgcgg catgccgcag atcgaagtta ccttcgatat
     1621 cgatgctgac ggtatcctgc acgtttccgc gaaagataaa aacagcggta aagagcagaa
     1681 gatcaccatc aaggcttctt ctggtctgaa cgaagatgaa atccagaaaa tggtacgcga
     1741 cgcagaagct aacgccgaag ctgaccgtaa gtttgaagag ctggtacaga ctcgcaacca
     1801 gggcgaccat ctgctgcaca gcacccgtaa gcaggttgaa gaagcaggcg acaaactgcc
     1861 ggctgacgac aaaactgcta tcgagtctgc actgactgca ctggaaactg ctctgaaagg
     1921 tgaagacaaa gccgctatcg aagcgaaaat gcaggagctg gcacaggttt cccagaaact
     1981 gatggaaatc gcccagcagc aacatgccca gcagcagact gccggtgctg atgcttctgc
     2041 aaacaacgcg aaagatgacg atgttgtcga cgctgaattt gaagaagtca aagacaaaaa
     2101 ataatcgccc tataaacggg taattatact gacacgggcg aaggggaatt tcctctccgc
     2161 ccgtgcattc atctaggggc aatttaaaaa agatggctaa gcaagattat tacgagattt
     2221 taggcgtttc caaaacagcg gaagagcgtg aaatcaaaaa ggcctacaaa cgcctggcca
     2281 tgaaatacca cccggaccgt aaccagggtg acaaagaggc cgaggcgaaa tttaaagaga
     2341 tcaaggaagc ttatgaagtt ctgaccgact cgcaaaaacg tgcggcatac gatcagtatg
     2401 gtcatgctgc gtttgagcaa ggcggcatgg gcggcggcgg ttttggcggc ggcgcagact
     2461 tcagcgatat ttttggtgac gttttcggcg atatttttgg cggcggacgt ggtcgtcaac
     2521 gtgcggcgcg cggtgctgat ttacgctata acatggagct caccctcgaa gaagctgtac
     2581 gtggcgtgac caaagagatc cgcattccga ctctggaaga gtgtgacgtt tgccacggta
     2641 gcggtgcaaa accaggtaca cagccgcaga cctgtccgac ctgtcatggt tctggtcagg
     2701 tgcagatgcg tcagggtttc tttgccgtgc agcagacctg tccacactgt cagggccgcg
     2761 gtacgctgat caaagatccg tgcaacaaat gtcatggtca tggtcgtgtt gagcgcagca
     2821 aaacgctgtc cgttaaaatc ccggcagggg tggacactgg agaccgcatc cgtcttgcgg
     2881 gcgaaggtga agcgggccag catggcgcac cggcaggcga tctgtacgtt caggttcagg
     2941 ttaaacagca cccgattttc gagcgtgaag gcaacaacct gtattgcgaa gtcccgatca
     3001 acttcgctat ggcggcgctg ggtggcgaaa tcgaagtacc gacccttgat ggtcgcgtca
     3061 aactgaaagt gcctggcgaa acccagaccg gtaagctttt ccgtatgcgc ggtaaaggcg
     3121 tcaagtctgt ccgcggtggc gcacagggtg atttgctgtg ccgcgttgtc gtcgaaacac
     3181 cggtaggcct gaacgagaag cagaaacagc tgctgcaaga gctgcaagaa agcttcggtg
     3241 gcccaaccgg cgagcacaac agcccgcgct caaagagctt ctttgatggt gtgaagaagt
     3301 tttttgacga cctgacccgc taacctcccc aaaagcctgc ccgtgggcag gcctgggtaa
     3361 aaatagggtg cgttgaagat atgcgagcac ctgtaaagtg gcggggatca ctccccgccg
     3421 ttgctcttac tcggattcgt aagccgtgaa aacagcaacc tccgtctggc cagttcggat
     3481 gtgaacctca cagaggtctt ttctcgttac cagcgccgcc actacggcgg tgatacagat
     3541 gacgatcagg gcgacaatca tcgccttatg ctgcttcatt gctctcttct ccttgacctt
     3601 acggtcagta agaggcactc tacatgtgtt cagcatatag ggggcctcgg gttgatggta
     3661 aaatatcact cggggctttt ctctatctgc cgttcagcta atgcctgaga cagacagcct
     3721 caagcacccg ccgctattat atcgctctct ttaacccatt ttgttttatc gattctaatc
     3781 ctgaagacgc ctcgcatttt tgtggcgtaa ttttttaatg atttaattat ttaactttaa
     3841 tttatctctt tatcgcaatt attgacgaca agctggatta tttttgaaat attgtcctaa
     3901 caaataacgc cgactgacaa gaaattaatt attacttttc ctaattaatc cctcagaaat
     3961 cctcacctta agctatgatt atctgagctt agggtcactc gtcagcgctt acagccgtca
     4021 aaaactcatc tcaccgctga tggcgaaaat tctacaaagg ctcgtaaaaa acgaattatt
     4081 cctacactat aatctgattt taacgatgat tcgtgcgggg taaaatagta aaaacgattt
     4141 attcacctga aagagaaata aaaagtgaaa catctgcatc gattctttag cagtgatgcc
     4201 tcgggaggca ttattcttat cattgccgct atcctggcga tgatgatggc caacagcggc
     4261 gcaaccagtg gatggtatca cgactttctg gagacgccgg ttcagctccg ggttggttca
     4321 ctcgaaatca acaaaaacat gctgttatgg ataaatgacg cgctgatggc ggtatttttc
     4381 ctgttagtcg gtctggaagt taaacgtgaa ctgatgcaag gatcgctagc cagcttacgc
     4441 caggccgcat ttccagttat cgccgctatt ggtgggatga ttgtgccggc attactctat
     4501 ctggccttta actatgccga tccgattacc cgcgaagggt gggcgatccc ggcggctact
     4561 gacattgcct ttgcacttgg agtgctggcg ctgttgggaa gtcgtgttcc gttagcactg
     4621 aagatctttt tgatggctct ggctattatc gacgatcttg gggccatcat tatcatcgca
     4681 ttgttctaca ctaatgactt atcgatggcc tctcttggcg tcgcggctgt agcaattgcg
     4741 gtactcgcgg tattgaatct gtgtggtgta cgccgcacgg gcgtctatat tctggttggc
     4801 gtagtgttgt ggactgcggt gttgaaatcg ggggttcacg caaccttggc gggggtaatt
     4861 gtcggcttct ttattccttt gaaagagaag catgggcgtt ctccagcgaa gcgactggag
     4921 catgtgttac acccgtgggt ggcgtatctg attttgccgc tgtttgcatt tgctaatgct
     4981 ggcgtttcac tgcaaggcgt cacgctggat ggcttgacct ccattctgcc attggggatc
     5041 atcgctggct tgctgattgg caaaccgctg gggattagtc tgttctgctg gttggcgctg
     5101 cgtttgaaac tggcgcatct gcctgaggga acgacttatc agcaaattat ggcggtgggg
     5161 atcctgtgcg gtatcggttt tactatgtct atctttattg ccagcctggc ctttggtagc
     5221 gtagatccag aactgattaa ctgggcgaaa ctcggtatcc tggtcggttc tatctcttcg
     5281 gcggtaattg gatacagctg gttacgcgtt cgtttgcgtc catcagtgtg acaggacggt
     5341 ttaccgggga gccataaacg gctccctttt cattgttatc agggagagaa atgagcatgt
     5401 ctcatatcaa ttacaaccac ttgtattact tctggcatgt ctataaagaa ggttccgtgg
     5461 ttggcgcagc ggaggcgctt tatttaactc cacaaaccat taccggacag atccgggctc
     5521 tggaagagcg cctgcaaggc aaattattta agcgcaaggg acgtggtctc gaacccagcg
     5581 agctgggaga actggtctat cgctatgccg ataaaatgtt caccttaagc caggaaatgc
     5641 tggatattgt gaactatcgc aaagaatcca atttattgtt tgacgttggc gtggctgatg
     5701 cactttccaa acgcctggtc agtagcgtgc tgaatgctgc agtggtagaa ggcgagccga
     5761 ttcatcttcg ctgcttcgaa tccacccacg aaatgctact ggagcaatta agccagcata
     5821 aactggatat gatcatttcc gactgtccga tagactctac gcagcaggaa ggcctgttct
     5881 ccgtgagaat tggcgaatgt ggcgtgagtt tctggtgtac aaatccacca ccagaaaaac
     5941 cgttcccggc ttgtctggaa gaacggcgac ttttgattcc tgggcgacgt tcaatgttag
     6001 ggcgcaaatt gcttaactgg tttaactccc agggattaaa cgtagaaatc ctcggcgagt
     6061 ttgatgatgc cgcgttgatg aaagcttttg gtgcaatgca caatgcaatc ttcgttgccc
     6121 caacgcttta tgcatatgac ttttatgccg ataaaactgt cgtagaaatt ggtcgcgtcg
     6181 agaatgtgat ggaagagtac catgctattt ttgctgagcg gatgattcag cacccggcgg
     6241 tacagcgaat ctgcaatacg gattattctg cgctttttag tccagcggcg cgttaatcgg
     6301 cagctccccc aaagttaaag tggaggagat agattagttg tacataacca cgattttgac
     6361 tcggctcatt atttgcccgc ttgagacatt atttccatat gtacgcgggc gaataaatag
     6421 aggaatctga ttacttcctt catggggatg
//
LOCUS       DAJVTY010000195         6369 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVTY010000195 DAJVTY010000000
VERSION     DAJVTY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.195"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..195)
                     /locus_tag="OQQ66_003693"
     CDS             complement(<1..195)
                     /locus_tag="OQQ66_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS1906443.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISV"
     gene            674..2152
                     /locus_tag="OQQ66_003694"
     CDS             674..2152
                     /locus_tag="OQQ66_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCS1906444.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IEHWCITFYICIPVVWDRFILALLPMSSKYCVSIVGPETCAS"
     gene            2035..2451
                     /locus_tag="OQQ66_003695"
     CDS             2035..2451
                     /locus_tag="OQQ66_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial regulatory, Fis family protein"
                     /protein_id="HCS1906445.1"
                     /translation="MHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLME
                     YLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMI
                     REALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2491..2970)
                     /gene="xdhC"
                     /locus_tag="OQQ66_003696"
     CDS             complement(2491..2970)
                     /gene="xdhC"
                     /locus_tag="OQQ66_003696"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1906446.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2967..3845)
                     /gene="xdhB"
                     /locus_tag="OQQ66_003697"
     CDS             complement(2967..3845)
                     /gene="xdhB"
                     /locus_tag="OQQ66_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1906447.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQMMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3856..6153)
                     /gene="xdhA"
                     /locus_tag="OQQ66_003698"
     CDS             complement(3856..6153)
                     /gene="xdhA"
                     /locus_tag="OQQ66_003698"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1906448.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1590 a         1506 c         1607 g         1666 t
ORIGIN      
        1 tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc
       61 ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt
      121 taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc
      181 attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagtg gtttatttca
      241 ctgtgacttg ccaaagtgat tggtgttttc acagctatac ctgggactaa caataagtgt
      301 gccagaatta tccgcgcggc tgcaaaagac taatttgacg gataatgtgt ggggttgatc
      361 acaaattgat gccagcttct ggttgtttgt aaaatacgaa aacgtttggc tttttttatc
      421 gcgttattta tttgctgcta agtatcagaa ataagataaa ggggcaggct atctctcttc
      481 agtttgataa actcaccgcc gtaattctca tattgatata gaaatgtgat atctcttcca
      541 taatttcatt agaaaatgat aaaaatctta tgtttttatc aaagccaatt tcgcctgagt
      601 taatatcatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt aagtaagtct
      661 ggaaggtaaa gctatggagc ttgctactac gcagtcagta ttgatgcaaa ttcaaccgac
      721 aattcagcgt tttgccagaa tgcttgccag cgttttgcag cttgaggttg agatcgttga
      781 tgaaaacttg tgtcgcgtcg ccggaacggg cgcgtatggg aagtttcttg gtcgccagtt
      841 gagcggcaac tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa aagttgtgac
      901 acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt gccgtgaaaa
      961 agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga taagtttgat
     1021 tgccgttacc cacgagcaac aagagcatat cagtgataat ttacgcgaat tttctgatta
     1081 tgttcgccat atatccacca tttttgtttc gaaacttctg gaggatcagg ggccaggaga
     1141 taacatcagt aaaatattcg cgaccatgat cgataatatg gatcagggcg tattagttgt
     1201 tgatgatgaa agtcgggttc agtttgttaa tcagactgcc ttaaaaacac ttggtgttgt
     1261 acaaaataat attattggga aacctatccg tttcagacca ttaacatttg agagtaattt
     1321 tactcatgga catatgcagc atattgtttc gtgggacgat aaaagtgaat taatcattgg
     1381 tcaattgcat aacattcagg gccgacaatt atttttaatg gcatttcacc aatcgcatac
     1441 cagtttttct gtagcaaatg cacctgatga accacatatt gaacaattgg ttggcgagtg
     1501 ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc catccagcgt
     1561 tatggtggtt ggtgaaagcg gcacgggtaa agaagtcgtc gcccgagcaa tccataagtt
     1621 gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc cggagcagct
     1681 tctggaaagc gaactgttcg gttatgttaa aggcgcattt actggcgctt ctgccaacgg
     1741 taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg aaataggtga
     1801 tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcgcgtg aaattctgcc
     1861 gattggtgcc agtagcccaa tacaagtcga cattcgcatc atttctgcaa ctaatcagaa
     1921 tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc gacttaatgt
     1981 tatcccgata actctgccac cgctgcgtga acgtcaggaa gatattgaac attggtgcat
     2041 tactttttac atctgcatac ccgtcgtctg ggatcggttt atcctggcat tgctcccgat
     2101 gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt aagcaatttg
     2161 atggaatatc tggttaacgt agttccttca ggtgaagtta tcgacagcac gctattgccg
     2221 ccaaatctgc tgaataatgg cacaacggag caaagtgatg taacagaggt cagtgaggcg
     2281 cacctgtcac tcgatgatgc gggcggcacg gcgctggagg agatggaaaa gcaaatgatc
     2341 cgcgaggcgc tttcacgtca taacagcaag aagcaagttg ctgatgaact gggcatcggc
     2401 attgctacgc tctatcgcaa gattaagaaa tatgagttgt taaacacata acctcagtga
     2461 atccggcctg aattcaggcc ggatatcctt ttacttcgtt ttctcgcaat ccagaactgt
     2521 atttacaatc atctgatacc ccgtgcagcg acaaagattt cccgccagtc cgcgacgaat
     2581 ttccgtaatg gttaatggct tctcgcgtgg tttcgccagc attgccgtgg tagccataat
     2641 caggccaggc gtacaaaacc cgcactgcac tgcgccggat ttcgcataag cctgctgaac
     2701 atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg cggatctctt ttccttcagc
     2761 ccaggcggca aggtataagc aactgtctat tgctgtgccg tcgaccaaca ccgtacaggc
     2821 accacattca cccacgcagc acccttgttt gacacttagc agtccttgtt cgcggagtaa
     2881 ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa ggcatcccgt taatggtgca
     2941 ttcgatggta attgtttcgc tgtgattcat tgcaattttc cccccgccgc ggcgacggct
     3001 tcgctaatca cttttttggt catcatctgg atgagatgca gacgaaactc tttactggcc
     3061 cgccatgaag aacgcggggc gacatcttgc aggacagatt cgctgatagc ttccagcgtt
     3121 tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat gttggcagcg aatcggcgtt
     3181 ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat tgccgttatc cagtcggcaa
     3241 tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc gcatggcata tttaaaatgc
     3301 gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa aggcgacgag gatttcgtca
     3361 tgctcaagag acactttgcc cggcccggtg tgaaagccat taatcgggac gaaacgaaca
     3421 ccgcgcgggg agtggatctc cagtttcgcg tcataaatta gcgttggcgt ggcagaatct
     3481 gcgctggtgg caccgttgca aatatttcca ccgtaggtag cgacgttacg gatctgcagt
     3541 ccagcaatgg acatggccgc agcacataac gccgggagat gacgttgagt tataggatct
     3601 tctattagct gggtaaatgt cgttgcagag ccgatacgta gcgagccatc ttccgccagc
     3661 gtaattcccc gcagctccgc cagattatga atatcaacaa tatggcgata acggtcattg
     3721 tgatggtgga gctgaatcag tacgtcagtg ccaccggcga gcagtttggc ctgcgggttg
     3781 ttagccagca ggttgatggc atcggcaagg gttgctgcgc gatggtaaga agcaaaatca
     3841 aacatgatgt tatcctcaaa tcaatcctgc cagatggaac tcttcatata accgttttgg
     3901 cgtcagcggc agtgtattga ttgcaacacc ggtagccatc ttcaccgcgt tacgaatagc
     3961 agcggcaaca ggaattattg gtggctcacc cagtgactta tgtccgtatg cggattgcgg
     4021 ctcattgatt tcgacgaacg cgctttccag ttgtggcaga tccggcatgg tcggcatttt
     4081 gtaatccagc agattggggt tacggaccac gccgctttta gcatcgatga tcatctcttc
     4141 aaatagcgcc cagccaatgc ccattcccat tccgccgtgt acctgacctt ctgccagcag
     4201 tggattaaga atatgccctg aatcatgaac gttgaggatg cggttgatgg tgactttgca
     4261 cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca aacgccggtg ggttagtggt
     4321 ggttttgatg gagctttcag cagagagctg cccgccgcgt tcagggtggt agaaagcgtc
     4381 catcgccaaa tcttttaacg acattaacgg ctcttccggt cgttcaacca gcacgatatg
     4441 gccttttatc agggtcagat tcatcgctga ctgatgtagc atgactgcgg cgtgagcgat
     4501 gattttctct tttaataata gtgccgcact gcgcagcgca ggcgcggcaa catagctctg
     4561 gcgtgaggca aatgtgccgg gatcgaacgg cgtaacgtcg gtatcttgag ttgaaataac
     4621 gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc atttgcgaga agacggtgtc
     4681 ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg atggttccat cctgattcat
     4741 cagaaggcgc gcgcctgcta tttctacgcc gacaggccag gtgttagagg tgtagctaaa
     4801 acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg ttctggcatt ctgcacggcg
     4861 tttttcccat tcaaagattt tccggccttt ttcaagacac tccggcaacc ctgcgctgta
     4921 aatacgtttg cccgtgagcg gattagcatc tccttcgcgg gcggcgttgc gtaaacgaat
     4981 ttcaacagga tcaataccta acgctgtcgc ggcgtcatca agcatagact caacggcaaa
     5041 tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc gaggggaggt tggtatagca
     5101 ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg taagcgactt tattcccccc
     5161 cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg gtgttagaca gaacatccag
     5221 actataacct ttcaatgttc cgtcgcggtt cacgcccatt tgcccgtcaa tggtaaaagc
     5281 gtggcgggta cgggttgcga ggaaacactc ttcacggcta agggaaactt tcaccggaat
     5341 gccgccaagc ttgctggtca ggaatgccgc cattggctct tccagtacat cctgtttatt
     5401 accaaaaccg ccaccgacaa atggtttgat gactcgtacg catgaccagg gaatatccag
     5461 cgcctgacca accacgcggc gaacaatgtg cgggatctgg gtgctggaaa cgatggtaat
     5521 tcgcgagtca tcctccatcc acgccagcga tgttacgctt tccatgtgac aatgttgaat
     5581 aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg gcatcgattg tttgttggac
     5641 attacccgtc gacatcgtgc tttgtttcag taaattgcca ccgttatgga ttggtgcagc
     5701 gtcttctgcc agcgccgctt ctggcgtggt gataacgggt aattcttgcc actcaatgct
     5761 gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg gccacgacga tggcaatggc
     5821 gtcaccatga tgacgaacat ggcgagttag cagtgcgcga tcggcggtat cgcgcttgtt
     5881 ttcgtcaagt gtccaggcat gcccagctgt agcgaatgga atatcaggca catcttccca
     5941 ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt tcatcattaa tacttacggc
     6001 ataaccatgt gcgatagggc tacgtacata tttcgcataa cacatgcccg ccataacata
     6061 atcgtcagta tatcgtgccc gcccggtgac cttagcaatg gcatcgacgc gcatgcatga
     6121 ttcacccgta gcggttgctt cccgcgcttc catagaaatc ccctcgattt gaacatttgg
     6181 ttcttatggc aaagatttag tctaagcagg caagatttac gccagaaacg acgataagcg
     6241 ttaggggagg taggaagtgt gatgacgtta aaaaaacaca gatatgatac gaaacaggag
     6301 tctgaatagg ggcagtgata cgtatcaata tgagtggcga taaaatcaat gtgataaatt
     6361 gggattatt
//
LOCUS       DAJVTY010000196         6363 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVTY010000196 DAJVTY010000000
VERSION     DAJVTY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.196"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            27..173
                     /locus_tag="OQQ66_003699"
     CDS             27..173
                     /locus_tag="OQQ66_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1906449.1"
                     /translation="MQHGHIAQRGNHDELAQQSGWYRDMYRYQQLEAALDDAPEIREE
                     AVDA"
     gene            166..1947
                     /locus_tag="OQQ66_003700"
     CDS             166..1947
                     /locus_tag="OQQ66_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1906450.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            2128..2466
                     /gene="glnK"
                     /locus_tag="OQQ66_003701"
     CDS             2128..2466
                     /gene="glnK"
                     /locus_tag="OQQ66_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1906451.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            2496..3782
                     /gene="amtB"
                     /locus_tag="OQQ66_003702"
     CDS             2496..3782
                     /gene="amtB"
                     /locus_tag="OQQ66_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1906452.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(3831..4691)
                     /gene="tesB"
                     /locus_tag="OQQ66_003703"
     CDS             complement(3831..4691)
                     /gene="tesB"
                     /locus_tag="OQQ66_003703"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1906453.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            4909..5481
                     /locus_tag="OQQ66_003704"
     CDS             4909..5481
                     /locus_tag="OQQ66_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1906454.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(5512..5823)
                     /locus_tag="OQQ66_003705"
     CDS             complement(5512..5823)
                     /locus_tag="OQQ66_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1906455.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            5986..6082
                     /gene="ffs"
                     /locus_tag="OQQ66_003706"
     ncRNA           5986..6082
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQQ66_003706"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6202..>6363
                     /locus_tag="OQQ66_003707"
     CDS             6202..>6363
                     /locus_tag="OQQ66_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1906456.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDF"
BASE COUNT         1385 a         1588 c         1843 g         1547 t
ORIGIN      
        1 gggatgcgtt catataaatt attgtgatgc agcacggaca tatcgcccag cgtggcaatc
       61 atgatgagtt ggcgcaacaa agcggctggt atcgcgatat gtatcgctat caacaactgg
      121 aggcggcgct cgacgacgct ccggaaattc gcgaggaggc cgtcgatgcg tagttttagc
      181 caactgtggc cgactctcaa gcgtctgtta gcgtacggtt cgccgtggcg taaaccgctg
      241 gggattgcgg tcctgatgat gtgggttgcg gcagcggcag aagtcagtgg gccgctgctt
      301 atcagctatt ttatcgacaa tatggtagcg aaaaataacc tgccgttgaa aatggttgca
      361 gggctggctg cggcgtatgt tgggctacaa ctgtttgccg ccgggctaca ttacgcgcag
      421 tcgctgctgt ttaatcgggc ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg
      481 gatgccgcat tgcgccagcc attaagcgag tttgataccc aacccgtcgg gcaggtgatt
      541 tcccgcgtca ctaatgacac tgaagtgatc cgcgatctct acgttaccgt agtggcaact
      601 gtcctgcgta gcgccgcact ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg
      661 cgaatggcgc tggtggcgat aatgattttc ccggtggtgc tggtggtaat ggtgatatac
      721 cagcgttaca gcacgccgat tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac
      781 ggctttaacg aaatcatcaa tggcatgagc gttatccagc agttccgtca gcaggcgcga
      841 tttggcgaac gtatggggga ggccagtcgt tcacactata tggccaggat gcaaaccctg
      901 cgcctcgacg gttttctgct gcgtccgctg ctgagtctgt tttcatcgct cattctttgt
      961 ggcttgttga tgctgtttgg cttctccgct agcggcacca ttgaagtagg cgtgctgtat
     1021 gcgtttatca gctatcttgg gcgacttaac gaacccttaa tcgaactgac cacgcaacag
     1081 gcgatgctgc aacaggctgt tgttgctggt gagcgcgtgt ttgaactgat ggacggaccg
     1141 cgccagcaat atggcaatga tgatcgcccg ttacagagtg gcaccatcga agtcgataac
     1201 gtgtcatttg cttatcgcga tgacaatctg gtgctaaaga acattaatct ctctgtgcct
     1261 tcgcgtaact ttgtggcact ggtcgggcat accggcagtg gcaaaagcac cctcgccagt
     1321 ttattgatgg gctattaccc gctaacggaa ggtgagattc gccttgatgg tcgtccatta
     1381 agttcgctaa gtcacagcgc gctgcgccag ggcgtggcaa tggtgcagca agatccggtg
     1441 gtgctggcgg ataccttcct cgccaacgtg acgctggggc gtgatatctc cgaagaacgc
     1501 gtctggcagg cgctggaaac cgtgcaactg gcggagctgg cgcgcagcat gagtgatggt
     1561 atttatacgc cgctgggcga gcaggggaat aatctctccg tcgggcaaaa gcaactgctg
     1621 gcactggcac gcgtgttggt ggagacgccg caaatcctga tccttgatga ggcaaccgcc
     1681 agcattgact ccggtactga acaggcgatt caacatgctc tggcggctgt gcgtgaacat
     1741 accacgctgg tggtgattgc tcaccgctta tcgaccattg ttgatgccga caccattctg
     1801 gtgcttcatc gtgggcaagc cgtggagcag ggcactcacc agcaactgct ggcggcccag
     1861 ggacgctact ggcagatgta tcaactgcaa cttgcgggcg aagagctggc agccagcgtg
     1921 cgtgaagaag aatcattgag cgcctgaata gcgcaatatt tcatcgttgg tgcaaaaatg
     1981 taacgcactg tgcactgtca tagtgcgttt tcattttcaa tcttcttaac ttcctgctct
     2041 ctttctcgtt tttcatttct ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca
     2101 ttaccgaatt ctgactggag gggatctatg aagctggtga ccgtgataat caaaccattc
     2161 aagctggaag acgttcgtga agcgttatct tccattggta ttcagggcct gaccgtcacc
     2221 gaagtgaaag gtttcgggcg tcagaaaggg catgccgagc tgtaccgggg ggcggaatac
     2281 agcgtcaact tcctgccaaa agtaaaaatt gatgtggcaa ttgctgatga ccaactcgat
     2341 gaagtgatcg atatcgtcag taaggcggct tacaccggaa aaattggcga cggcaaaatc
     2401 ttcgtcgctg aattgcaacg cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg
     2461 ctgtaatctc tggcacacag caacaggaac gaaaaatgaa gatagcgacg ataaaaactg
     2521 ggcttgcttc actggcgatg ctgccgggac tggtaatggc tgcacctgcg gtggccgata
     2581 aagccgacaa tgcgtttatg atgatttgta ctgcgctggt gctgtttatg actattccgg
     2641 ggattgccct gttttacggt gggttgattc gcggcaaaaa cgtgctgtcg atgctgatgc
     2701 aggtgacggt gacgtttgca ctggtctgta ttctctgggt ggtttacggt tactcgctgg
     2761 cgtttggtga gggtaacaac ttcttcggca acgttaactg gttgatgctg aaaaacatcg
     2821 aactgacggc ggtgatgggc agcatttatc agtatatcca cgtagcgttt cagggatcgt
     2881 ttgcctgcat taccgtaggc ttgatagttg gggcgctggc ggaacgaatc cgcttctcag
     2941 ctgtgttgat tttcgtggtg gtatggctga cgctctctta cattccgatt gcgcatatgg
     3001 tgtggggcgg cggtttgctg gcttctcacg gtgcactgga tttcgcgggt ggcacagtgg
     3061 tgcacattaa cgccgcaatc gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg
     3121 gcttcggtaa agaggcgttt aaaccgcaca acctgccgat ggtcttcacc gggactgcca
     3181 ttctctatat cggttggttt ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg
     3241 cggcactggc atttgtgaat actgtggtcg caacggcggc ggcaattctt ggctggatct
     3301 tcggtgaatg ggcgctgcgt ggtaagcctt cactgctggg ggcgtgttct ggcgcgattg
     3361 ccggtctggt cggcgtgacg ccagcgtgcg gctacattgg ggttggcggc gcgttgatta
     3421 tcggcgtggt agctggtctg gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc
     3481 gggtggatga tccctgcgat gtcttcggtg tgcacggcgt ttgtggcatt gtcggctgta
     3541 tcatgaccgg gatttttgcc gccagctcgc tgggcggcgt gggcttcgct gaaggtgtga
     3601 cgatgggtca tcagttgctg gtacagctgg aaagcatcgc cattacgatc gtctggtccg
     3661 gtgtggtggc atttattggc tacaaattgg cggatctgac ggttggtctg cgtgtaccgg
     3721 aagagcagga gctagaaggg ctggatgtca acagtcacgg cgagaatgcc tataacgcgt
     3781 aacaagcact gcaaaaaaca gccggacggt tttcacctcc ggctattttt ttaattgtga
     3841 ttacgcatca ccccttcctg aacggtcgag gcaaccagta cgccgtcctg ggtataaaac
     3901 tcaccgcgca caaagccacg tgcgctggac gccgaggtgc tctccacgct atacagcagc
     3961 cattcattca aattaaacgg gcgatggaac cacatggaat ggtcaatggt ggcaatctga
     4021 atccccggtt cgagaaaacc gatgccgtgc ggctgtagag ctaccggcag gaagttaaga
     4081 tcagaagcgt aaccgagcag atactgatga acgcgcaggt catccggcac gctaccattt
     4141 gcgcggatcc acacctgacg atgtggttct gcgacgtgac ctttcagtgg gttatgaaac
     4201 tccaccggac ggacttccag cggacgatcg cagatgaatt tatctttaag cactggcggc
     4261 agcaggtgcg ccagcgattg ggcgatttgc gtttccgaag gcaggccatc aggcgctggc
     4321 gcggacggca ttgttttttg atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc
     4381 atataaaaaa tcggtttgcc gttttgaata gcagcaaccc ggcgggcgct gaagctgtta
     4441 ccatcacgca gcgtttcgac atcataaata atcggtttct tactatcgcc tgggcgaaga
     4501 aagtagctgt gaaacgaatg taccagccgc tcttccggga cggtctcttt tgcagcatac
     4561 aaggcctgac ccacgacctg gccgccaaac acctggcgta aacctaaatc ttcactctgg
     4621 ccgcgaaaga gtcctgcctc aattttttcc agatttaaca atgccagtaa attttttagc
     4681 gcctgactca tataactctc cagtaacaaa gctgccgcag caagccaaag tgagttgagt
     4741 ataacgcaaa tttgccactg gtccgatggg tgcaataatc tgaattacgg gctaattaca
     4801 ggcagaaatg cgtgatgtgt gccacactta ttgacgttac tattttgtta accactcttc
     4861 cggcgaggaa agttagcccg ctggtgcatt gataataagg agaaatgaat gaaactcgtg
     4921 cacatggcca gtggtttagc ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg
     4981 gatattcaga cgccagcccc ggctgcaaat acgtctattt cagcaacaca acaacctgct
     5041 atccagcaac cgaatgtctc cggtaccgtc tggatccggc agaaagtcgc actgccgcct
     5101 gatgctgtgc tgaccgtgac actttctgac gcgtcgttag ccgatgcacc gtcaaaagtg
     5161 ctggcgcaga aagcggtgcg taccgaaggt aaacagtcac cattcagctt tgttctgcca
     5221 tttaacccgg cagatgttca gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg
     5281 aatgacaaac tggtatttat taccgatacc gttcagccgg tgatcaacaa gggcggaact
     5341 aaagccgacc tgacattggt gccggtgcag caaaccgccg tgccggttca ggccagcggt
     5401 ggtgcaacga ctaccgtacc ttcgacttca ccaactcagg tgaatccgtc ttcggcagtt
     5461 ccagctccta cgcaatatta agcgacgtta accctctccg cccggagagg gttagtagtt
     5521 ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac cttctgccag
     5581 taatgcctgt cgctgacgct gtaaatccgg tccggttagc gaaattgtgc cgtggcgatt
     5641 aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca caccgcccac
     5701 ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag tggtgacata
     5761 gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat cttctttttc
     5821 catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg agtgaagtcg
     5881 cattgcgcaa gaaaccagca tctggcacgc gatgggttgc aattagccgg ggcagcagtg
     5941 ataatgtgcc tgcgcgttgg ttctcaacgc tctcaatggg ggctctgttg gttctcccgc
     6001 aacgctactc tgtttaccag gtcaggtccg gaaggaagca gccaaggcag atgacgcgtg
     6061 tgccgggatg tagctggcag ggcccccacc catttctgcc tcccaccgtt tcgtcaaaaa
     6121 aaaccaacat ggctaaactt taaccacaac tgacgtcgca agaattgtct ggctgcgcag
     6181 tacgcttcgg aggtatgtct gatgaagtat gttgatggtt ttgtggttgc cgttcctgcc
     6241 gataaaaagg atgcctatcg ggaaatggcc gctaaggctg cgccattgtt taaagagttt
     6301 ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac cggatggcaa agtgaccgat
     6361 ttt
//
LOCUS       DAJVTY010000197         6337 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVTY010000197 DAJVTY010000000
VERSION     DAJVTY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.197"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            30..152
                     /locus_tag="OQQ66_003708"
     CDS             30..152
                     /locus_tag="OQQ66_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCS1906457.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            168..1013
                     /locus_tag="OQQ66_003709"
     CDS             168..1013
                     /locus_tag="OQQ66_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1906458.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1036..2547
                     /locus_tag="OQQ66_003710"
     CDS             1036..2547
                     /locus_tag="OQQ66_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1906459.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="OQQ66_003711"
     CDS             2572..3570
                     /locus_tag="OQQ66_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1906460.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="OQQ66_003712"
     CDS             3603..4697
                     /locus_tag="OQQ66_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1906461.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="OQQ66_003713"
     CDS             4709..5581
                     /locus_tag="OQQ66_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1906462.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="OQQ66_003714"
     CDS             complement(5629..6051)
                     /locus_tag="OQQ66_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1906463.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6337)
                     /locus_tag="OQQ66_003715"
     CDS             complement(6148..>6337)
                     /locus_tag="OQQ66_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCS1906464.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1467 a         1515 c         1835 g         1520 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg gggttcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccagg
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DAJVTY010000198         6272 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVTY010000198 DAJVTY010000000
VERSION     DAJVTY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.198"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(75..611)
                     /gene="ssb1"
                     /locus_tag="OQQ66_003716"
     CDS             complement(75..611)
                     /gene="ssb1"
                     /locus_tag="OQQ66_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1906465.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            865..3687
                     /gene="uvrA"
                     /locus_tag="OQQ66_003717"
     CDS             865..3687
                     /gene="uvrA"
                     /locus_tag="OQQ66_003717"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1906466.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3722..4078)
                     /locus_tag="OQQ66_003718"
     CDS             complement(3722..4078)
                     /locus_tag="OQQ66_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1906467.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4082..4498)
                     /locus_tag="OQQ66_003719"
     CDS             complement(4082..4498)
                     /locus_tag="OQQ66_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1906468.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4609..5322)
                     /gene="aphA"
                     /locus_tag="OQQ66_003720"
     CDS             complement(4609..5322)
                     /gene="aphA"
                     /locus_tag="OQQ66_003720"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1906469.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5723..5945
                     /locus_tag="OQQ66_003721"
                     /pseudo
     CDS             5723..5945
                     /locus_tag="OQQ66_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1584 a         1552 c         1562 g         1574 t
ORIGIN      
        1 gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat ataacctatt
       61 gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg ctcgttagac
      121 ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa ctgattgcca
      181 ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc gatattacca
      241 cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc gccaacgttc
      301 accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt acgggtacgc
      361 agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc cacttctgcc
      421 agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc gccggtcgct
      481 ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc accatttggc
      541 atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt gtttacgcct
      601 ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta aacgcgcgat
      661 tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga aacaaacctg
      721 gccagacatt gttacacaac actccggata atgcattcca atactgtata ttcattcagg
      781 tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca ccgggcaaaa
      841 aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc ccgcacccat
      901 aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt gaccgggctt
      961 tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg gcagcgccgt
     1021 tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga aaagccggac
     1081 gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc gacgtctcat
     1141 aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg tttgttgttc
     1201 gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc gcaaaccgtc
     1261 agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat gctactcgcg
     1321 ccaatcatta aagaacgcaa aggcgaacac accaaaacgc tggagaacct ggcaagccag
     1381 ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc gccgaaactg
     1441 gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt gcgtgacgat
     1501 cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg tggtacagcg
     1561 gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc caacttcgcc
     1621 tgcccaattt gcggctacag tatgcgtgaa ctggagccac gactgttttc gtttaacaac
     1681 ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt cgatcctgac
     1741 cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg ctgggatcgc
     1801 cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa gttcgacgtc
     1861 gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta cggttctggc
     1921 aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat ccgtcgtcat
     1981 ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga atccagtgcg
     2041 gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg cgaagggacg
     2101 cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc tgctatctcc
     2161 gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc tggtcagcgg
     2221 gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt cctcgttaac
     2281 gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg cggtgaagcc
     2341 cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat gtatgtactg
     2401 gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg tacgcttatc
     2461 catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga cgcaattcgc
     2521 gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg tgaagtggtc
     2581 gcagaaggtc cgctggaagc gatcatggcg gtgcctgaat cgttgaccgg gcagtacatg
     2641 agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc agaaaaagtg
     2701 ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac gctgccagtc
     2761 ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct gattaacgac
     2821 acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga accggcaccg
     2881 tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga ccaaagccca
     2941 attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac acctgtgcgc
     3001 gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg acgtttcagc
     3061 tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat caaagtggag
     3121 atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa acgctataac
     3181 cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct ggatatgacc
     3241 atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa gctgcaaacg
     3301 ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac actttctggt
     3361 ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac cgggcagacg
     3421 ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca gcaactcctc
     3481 gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga gcacaatctc
     3541 gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg cagtggcggc
     3601 ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc ttcgcatacg
     3661 gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg gccttttcct
     3721 ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact gcctgctgat
     3781 aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg agatacacgg
     3841 tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc tgctgacgta
     3901 gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt tctacttctt
     3961 tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag tcgttatgca
     4021 cgctctgttc tgcgcctagt tttgccatgc aatattgtag caactccgaa atggtcattt
     4081 tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg cgatgttccc
     4141 ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac ggcaatacaa
     4201 gcgatgttcc ctgcattgag gatttgatat gagaaggcat atcgtctgct ccctcatagt
     4261 catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc atgtcgtggc
     4321 gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt tgcagcaaca
     4381 gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga atttcatccg
     4441 ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga taccacatgt
     4501 gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa ggataaaggt
     4561 gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc agtattctga
     4621 attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt aggtagagtt
     4681 ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg taatatcatt
     4741 atcagaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg attttgtatt
     4801 ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag gaatatgaaa
     4861 attatccgcc agcgtttttg aaaccgtttc tgttttcgtc ggactacgac cagtcacaaa
     4921 gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga cctcttttgg
     4981 aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat ttttcagata
     5041 atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccgggc tggaaaaaag
     5101 taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga ggctattttc
     5161 aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg caacgttagt
     5221 accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt ttagagcgaa
     5281 caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc ctaaatagtc
     5341 agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc ataacgccag
     5401 ctgcagggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga tgattatatt
     5461 taacccagta aatacgttcc tgtctttttt gtataaaaaa tgtttattac tcgatgtaag
     5521 gatacattaa ttttacgttt attatgatga atcctctgca aatgagcgaa atttgataga
     5581 acatttatct gctgtacgat ttaatgaact taatgataca gcattacaaa aacaatcgaa
     5641 gtttataaag atgattcctg attgaccaga accctatcct acaatggtga ttaattttat
     5701 tactcgcgat ggagatgatg atatgaatat atcatatatg aatagcaaca aaacgacatc
     5761 tttacctgtt gagctagatg cactaaataa taaagatatc tcttatgcca aagatttttc
     5821 ttatgtcaaa gatttctttt tatacataga aacacagtta aaaattgcaa aagatttttg
     5881 tagacttgga gaagaagtat caagttctat tgcaagtaaa gtttttcacg catttattga
     5941 tttagttaac aaaatcaggg gtaagaaaga ctttatgtat atttgcacgc tttgctgttt
     6001 tgccgaggag gttaaaggtg attattctca ttacaggacc tttttatttg atattggtaa
     6061 tcaatataag gttaaactta cacaaagcgg aaaaaaagag ttctctttaa cattagaatt
     6121 taacgatact ataattgaat ctcagaaagt cacaggcaat aaggcaaagc atattcttga
     6181 ggatatagaa atattctatc gtaacaaacc cgatacttat tattaaattt aataatggtt
     6241 agatgtgcag aaattattac tccacacgaa aa
//
LOCUS       DAJVTY010000199         6029 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVTY010000199 DAJVTY010000000
VERSION     DAJVTY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.199"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            275..616
                     /gene="raiA"
                     /locus_tag="OQQ66_003722"
     CDS             275..616
                     /gene="raiA"
                     /locus_tag="OQQ66_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1906470.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    719..844
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            720..767
                     /gene="pheL"
                     /locus_tag="OQQ66_003723"
     CDS             720..767
                     /gene="pheL"
                     /locus_tag="OQQ66_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCS1906471.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            867..2027
                     /gene="pheA"
                     /locus_tag="OQQ66_003724"
     CDS             867..2027
                     /gene="pheA"
                     /locus_tag="OQQ66_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1906472.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OQQ66_003725"
     CDS             complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OQQ66_003725"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1906473.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OQQ66_003726"
     CDS             complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OQQ66_003726"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1906474.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4480..4845
                     /locus_tag="OQQ66_003727"
     CDS             4480..4845
                     /locus_tag="OQQ66_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1906475.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4995..5513
                     /locus_tag="OQQ66_003728"
     CDS             4995..5513
                     /locus_tag="OQQ66_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1906476.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5503..>6029
                     /locus_tag="OQQ66_003729"
     CDS             5503..>6029
                     /locus_tag="OQQ66_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1906477.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAIT"
BASE COUNT         1646 a         1532 c         1351 g         1500 t
ORIGIN      
        1 agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt tattctgtta
       61 gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct cgctttgaga
      121 tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga ttcgttgaca
      181 aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac ggtatgctga
      241 attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta ccagcaaaca
      301 aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca aactggaaaa
      361 atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac aagggtttgt
      421 tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta aacatgaaga
      481 tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca ataaactgca
      541 gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact tcgtcgaaga
      601 agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc gggcgcgttt
      661 tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag gaaacaaaca
      721 tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg ggaggcgttt
      781 cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg ggggcctttt
      841 ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg gcgctgcgag
      901 agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg cgcgaactgg
      961 ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat attgatcgtg
     1021 aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt
     1081 acattactcg cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc
     1141 tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca
     1201 aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca
     1261 ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg
     1321 actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc
     1381 tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc gaccattgtt
     1441 tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc catccgcagc
     1501 cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt gaatataccg
     1561 aaagtacgtc agcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt
     1621 tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga
     1681 atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg
     1741 accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc ggtgcgctgg
     1801 ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg gaatcacgcc
     1861 cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat
     1921 cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca atgaaggtat
     1981 tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg
     2041 gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc ctgacgcaat
     2101 aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg
     2161 cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat cgcctcgccg
     2221 aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg
     2281 gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc aaggcggtaa
     2341 atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc cagatgcagc
     2401 ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc
     2461 tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat
     2521 tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc caggctaccg
     2581 ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg
     2641 gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc tttcggtaaa
     2701 ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac aatcaccatt
     2761 ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc
     2821 tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg
     2881 acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc gttttcactg
     2941 gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtaccccc
     3001 agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa
     3061 ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc
     3121 gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat
     3181 tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg
     3241 atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga
     3301 tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct cgtggatatt
     3361 actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga
     3421 ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac
     3481 catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc
     3541 agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg gattcccctg
     3601 agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc
     3661 ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt ttttaaaacc
     3721 aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc
     3781 accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc
     3841 ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg
     3901 cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca
     3961 gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct
     4021 gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt ccagatcatg
     4081 aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga taatatctga
     4141 aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg gaaaagcggc
     4201 cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc
     4261 gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag
     4321 gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta cgcttttctt
     4381 tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt tacaccccct
     4441 ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc
     4501 cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc
     4561 ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg cagcgccata
     4621 aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct taaaggatat
     4681 gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg actttccggt
     4741 aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca tgaagtatgg
     4801 caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa accccacaaa
     4861 taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg acgatcgcat
     4921 gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagaag
     4981 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga
     5041 cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
     5101 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
     5161 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
     5221 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
     5281 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
     5341 cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
     5401 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg
     5461 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
     5521 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
     5581 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
     5641 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
     5701 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
     5761 gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc gtcctggcat
     5821 tacacccgta aggatccagg cgatactttc agcaatttca taagccactg gctcttcccc
     5881 gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc
     5941 gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt
     6001 attctgctgg catcaggcat cgcaattac
//
LOCUS       DAJVTY010000200         5866 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVTY010000200 DAJVTY010000000
VERSION     DAJVTY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.201"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..2070)
                     /locus_tag="OQQ66_003730"
     CDS             complement(<1..2070)
                     /locus_tag="OQQ66_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1906478.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLNAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYG"
     gene            2312..3373
                     /locus_tag="OQQ66_003731"
     CDS             2312..3373
                     /locus_tag="OQQ66_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS1906479.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3486..4985)
                     /gene="ansP"
                     /locus_tag="OQQ66_003732"
     CDS             complement(3486..4985)
                     /gene="ansP"
                     /locus_tag="OQQ66_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS1906480.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5252..>5866
                     /locus_tag="OQQ66_003733"
     CDS             5252..>5866
                     /locus_tag="OQQ66_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS1906481.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1548 a         1500 c         1413 g         1405 t
ORIGIN      
        1 accataattt cgtccgggcg aaggttcgta atatctcccg tttgactcat tgacaatgac
       61 agaaccaacg tattctttat cgaataaatt atcgacacga ccaaataaat ccacagttaa
      121 attgtggtaa ttgtatttat acccggtgaa taagccgacg agagtataag acggcgcttt
      181 tgccgtattt tcatcatctg ccataatatc gcccatataa cgcgcttccg tgcctgcata
      241 ccaaccatct tccggtacat aacctatcga cgcaaagccc atattacggg cgatccctgg
      301 catccgatta ccgttacaat cctgttcatt gcaaacattg ctgcgatagg tcgcatcaag
      361 ccaggtccag gacgcgttta cgcgaaaatc tcctgcgaaa cgttgatccc atgccagttc
      421 agcgccttga cgacgggtct ttccggcatt tttgtaagtc gtacgcccac cgctactgct
      481 atcgacaaca atttcatcat cagtgtcggt ctgaaacaat gcgagactaa gcagcccatc
      541 accaatacgc gttttactgc cgatctcaat tgtatcgttg gtggatggtt ttaaaccaaa
      601 gttcataccg ctttgcccat cagcacgata agacagctca ttaatcgtcg gcgtttcaaa
      661 acctcgcccg gctgccagat agatattcca ggcatcggtc attgcatatt ttaacgaacc
      721 ggcaggtagc catttatgat aactggcatc accgctgtca tcgccgttac ccggagtaac
      781 gtaatggtcg ttggaatcaa accacacgga gctgtagcgc acgccagcat tcagcgacag
      841 tttttcgctc agctgccact gcgtctgtaa atagggatcg atgttccaca tcagattgcg
      901 ttcgtcgcga cgcaactcac ctttttgccc gtactccggc atgccgctat tcaggcggaa
      961 gttattgtag cccttgcggt tttcactcat gttttcgtag ttcaggccgg tagtgaacgt
     1021 gaccggaacg cccagttcac cacggtgtgt ccagcggctg tctatcccct ggtaatggcg
     1081 ttgcagggta atcacgccgc ccgcatgtga cgggttaagt tgtggtgcca tgggtattga
     1141 ctggtactgg gtcgtttctc gctctccggc atacatcatc acactcatat catcttgcgc
     1201 gctcaggcta cgctcatagc gcaacccagc ctgagtttgc ttgatggttt ttcgcgtgtc
     1261 gtactgttct gcacgaggcg cttgttgtgg attagccttc cattctgctt tggttagccc
     1321 acctgggtca tctgctttga tatccacact attgaaaatc agacttaatt tgctggcttc
     1381 atcaatgcgt acgcccagtt tggcattggc taaatttttc tgtgcgccac tatggtcacg
     1441 atagccgtgg gtcgtaaaac gcgtggttga gacggtgtaa tcgacatcgc caggctgtgt
     1501 gccgtctccc gttgcgcccg ttgctttcag cccatagcgc cagctgccaa aactgccgta
     1561 gtaactactg gcttcaatgg ttggtggctg ttgtccggtc tgggtggtga cattcattac
     1621 cccaccagac gcgttgccat acagggcaga gaaggggcca cgcagcactt ccacattttg
     1681 cacactgctt aaatcgatgt tggatgtttg cccttgcccg tcgggcatgg tggcgggaat
     1741 accgtccaca tacaggcgaa taccgcgaat accgtaagtg gagcgggagc caaatccgcg
     1801 aatcgacagc tgtaaatctt gcgcatagtt ctgccggttt tgtacctgca aaccaggcac
     1861 gccggtcagt gattcggaca agttaatgcg cggtgttgcc aggcgcatct cctcgccatc
     1921 caccacgctt actgctgctg gggtatccag ttctgaaacc acctgcggtg cggcactgac
     1981 aatcatagtc tgttcatcag cggcaaaaac aacgggggaa aggacaagca gtgcgggcaa
     2041 aacggtctgt cggacggaaa aaatcttcat gaaaaaagcc aggttaagaa tgggaaaacg
     2101 ctgtcatggt aataaaattg taaacttatg caaaacgaaa cggcacgata cgttaattaa
     2161 ttgagaaaat tgtagtcgta acggcaagaa atgctccaca tttgagaaaa taatgattac
     2221 cattcccatt tataacaaga gcgtgacgat gattacgctt agcgaagcat tgtgaagcag
     2281 caaaaatatt ggttcatcaa agggagtcgt catgcattta cgtcatctgt tttcatcgcg
     2341 cctgcgtggt tcattactgt taggttcatt gcttgttgct tcatcattca gtacgcaggc
     2401 cgcagaagaa atgctgcgta aagcggtagg taaaggtgcc tacgaaatgg cttatagcca
     2461 gcaagaaaac gcgctgtggc tcgccacttc gcaaagccgc aaactggata aaggtggcgt
     2521 ggtttatcgt cttgatccgg tcactctgga agtgacgcag gcgatccata acgatctcaa
     2581 gccgtttggt gccaccatca ataacacgac tcagacgttg tggtttggta acaccgtaaa
     2641 cagcgcggtc acggcgatag atgccaaaac gggcgaagtg aaaggccgtc tggtgctgga
     2701 tgatcgtaag cgcacggaag aggtgcgccc gctgcaaccg cgtgagctgg tagctgatga
     2761 tgccacgaac accgtttaca tcagtggtat tggtaaagag agcgtgattt gggtcgttga
     2821 tggcgagaat atcaaactga aaaccgccat ccagaacacc ggtaaaatga gtaccggtct
     2881 ggcgcttgat agcaaaggca aacgtcttta caccactaac gctgacggcg aattgattac
     2941 catcgacacc gccgacaata aaatcctcag ccgtaaaaag ctgctggatg acggcaaaga
     3001 gcacttcttt atcaacatca gccttgatac cgccaggcag cgtgcattta tcaccgattc
     3061 taaagcggca gaagtgttag tggtcgatac ccgtaatggc aatattctgg cgaaggttgc
     3121 ggcaccggaa tcactggctg tgctgtttaa ccccgcgcgt aatgaagcct acgtaacgca
     3181 tcgtcaggca ggtaaagtca gtgtgattga cgcgaaaagc tataaagtgg tgaaaacgtt
     3241 cgatacgccg actcatccaa acagcctggc gctgtctgcc gatggcaaaa cgctgtatgt
     3301 cagtgtgaaa caaaaatcca ctaaacagca ggaagctacc cagccagacg atgtgattcg
     3361 tattgcgctg taatagatat ggcgagggtg aaaacggtga gacttgcctg ccttttatca
     3421 cggcgtatcc ggcactttcg tcagtaatct gaatttgccc ggcacgtagc cgggcaattt
     3481 tgcaattaac tcgccgtttc aggcttaaac acaatttcct gtttttcttc atcttcctcg
     3541 acgactggcg cagtgctgtg aatttcagca acgcgtttgc gcacaccaaa ccagcctata
     3601 accagcagaa taccgataat cggcagcgcc gcgatagtat aagtcccgtt cgggtaatcg
     3661 aacgccatca gcacaaggac actcagtaaa aacagtaatg tcagccagga agtgaagggc
     3721 gcgccaggca gtttaaaact gacatccgct gctttgcctt ctttaatcgc tttacgcagg
     3781 cgcatctggc acacgatgat aaacgcccat gaagcgatga ttcccagcga cgcgaagttc
     3841 aacacaatct caaatacgcg cgacggcacc agatagttga ggaatacgcc gacgacatac
     3901 acaaccagtg tcgctagaat cccggcatac ggcacatgct gacgactcat tttcgccata
     3961 aaactcggtg cggaaccgcc catcgccatt gagcgcagaa tacgtccggt gcagtacaga
     4021 cctgagttca ggctggagag ggcagcggtc agcaccacaa tgttcataat gctgccgata
     4081 tatggcacac ccagtttaga gaaaaacgtc acgaacggac tttgccccgc ctgatacgcg
     4141 ctccacggca ataacataac cagcaacacc acggagccga cgtaaaacag gccaatacgc
     4201 caaatcacac tgttaatggc tttaggcacc atggtctgcg gatctttaca ttctccggca
     4261 gctgtaccca ccatttcaat ggaggcaaaa gcaaacacta cgccctgaat caacaccagc
     4321 gcaggcagca aaccgtgggg gaagaagccg ccattatcgg tgattaaatg aaagccagtg
     4381 gtgttgccat ccagcggctg accactaccg aggaacactg tacccacgac caaaaaggtc
     4441 acaatggcga gcactttaat aagcgcaaac cagaactcca tctccgcaaa ccatttcaca
     4501 ccgatcatat tcatggtgcc aacgatggta agtgcagcga gcgcaaagac ccactgcggc
     4561 acgccgccaa acgcacccca gtaatgcata tacagagcga cggcggtaat atcaacaatc
     4621 cccgtcatcg cccagttgat gaagtacatc cagccagcaa cataagcggc tttctcaccc
     4681 aaaaactcac gggcataaga gacaaaactg ccactggaag ggcggtgtag caccagctca
     4741 cccaatgcac gcagaataaa aaacgaaaac aagccacaaa ttaaataaac cagtgccagt
     4801 gctggacccg ccatttgcag tcgggctcct gcacctaaaa acaagccggt gccaatcgcg
     4861 ccgccaatgg cgatcatctg tacctggcga ttgcccatcg ctttgtgata cccctcttcg
     4921 tgggcattaa gccagcggcg tttcgcggcg tgttgatctg aagtgtcggt gtcgtgttta
     4981 ctcattgctc tccctgattg ctttaatgaa aaagtcatat aagttgccat gaacaatgtt
     5041 tattcatcct ggcgatgata gttatcacca ttattcttta tgggtataaa actatcggcg
     5101 cagcatccta cccgttatcg atatacgatg caaaacatcc ccttacaatc ctgaagggga
     5161 ttcatacaac tgacgaaaaa atgacaaata cttttgctgg ttaacctgtg tactgtccta
     5221 cacttaatct ttaaaagatt gtgaggggca tatgattaag gtgtatggcg tacccggatg
     5281 gggctctaca atcagtgagc tgatgctaac actggctgat attccttatc agttcgttga
     5341 tgtcagtggt tttgaccacg agggaacttc acgcgagtta ttgaaaaccc tgaacccgtt
     5401 gtgtcagatt ccgacactgg cgctggaaaa tgacgagatt atgacggaga cagcggcgat
     5461 tgcattgatg gttctcgatc gtcgcccgga ccttgcgcca ccggttgggc gcgccgaacg
     5521 tcagcagttt caacggctat tggtctggct ggttgctaat gtctatccaa cattcacttt
     5581 cgccgattac cctgagcgtt gggctcctga cgcacctgaa cagttaaaga agaatgttat
     5641 tgaatatcgt aaatcgcttt atatctggct gaattcacaa ttgaccgctg aaccttatgc
     5701 gtttggtgag caactaacgc tggtggactg ttatctttgc actatgcgca catgggggcc
     5761 tgggcatgaa tggtttcagg ataatgccac gaatatcagt gcgattgctg atgctgtatg
     5821 tcagttaccg aaattacaag aagtattaaa aaggaatgaa attatt
//
LOCUS       DAJVTY010000201         5845 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVTY010000201 DAJVTY010000000
VERSION     DAJVTY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.202"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..933
                     /locus_tag="OQQ66_003734"
                     /pseudo
     CDS             1..933
                     /locus_tag="OQQ66_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1321..1697)
                     /gene="rnpB"
                     /locus_tag="OQQ66_003735"
     ncRNA           complement(1321..1697)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQQ66_003735"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1730..2875)
                     /gene="garK"
                     /locus_tag="OQQ66_003736"
     CDS             complement(1730..2875)
                     /gene="garK"
                     /locus_tag="OQQ66_003736"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1906482.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2972..3862)
                     /gene="garR"
                     /locus_tag="OQQ66_003737"
     CDS             complement(2972..3862)
                     /gene="garR"
                     /locus_tag="OQQ66_003737"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1906483.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3892..4662)
                     /gene="garL"
                     /locus_tag="OQQ66_003738"
     CDS             complement(3892..4662)
                     /gene="garL"
                     /locus_tag="OQQ66_003738"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS1906484.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4678..>5845)
                     /gene="garP"
                     /locus_tag="OQQ66_003739"
     CDS             complement(4678..>5845)
                     /gene="garP"
                     /locus_tag="OQQ66_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1906485.1"
                     /translation="YIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQ
                     GFVDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQY
                     FSLALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFI
                     SENGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFP
                     IYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGM
                     LLASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVF
                     GNVASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1548 a         1573 c         1319 g         1405 t
ORIGIN      
        1 gactcgtttc ttcgtctgga ttttttaggt tataacgccc aaattattca acgatcggac
       61 aatacctgcg aacttaccat taatgaaccg ttaaaaaacc aggaaatatc tacaggcaat
      121 atcaatgcta attgtccatt aaaagatatt tacaatgaaa tcaggaggtt gaacgcaacc
      181 tttagttgtg ggactggaga tatagttgat ctatcctctc tggacttacg taatgtcgat
      241 ttagattatt atgatttcac agataaacat atggctaata ctattctaaa tccttttaaa
      301 ttggattcaa caaattttac taatgccaac atgtttcagg ttaattttgt tagttcaaca
      361 caaaacgcca caatctcctg ggattattta ctaaaaataa cgcctgtttt aataagtatt
      421 agcgatatgt attctgaaga aaaaatcaag tttgtcgaaa gttgtttaaa tgagcttgga
      481 gacattaccg aagaacaatt aaaaattatg agatttgcaa ttataaaatc tataccaagg
      541 gcaactctta cagataaatt agaaaatgaa ttaacaaaag aaatatataa aagctcatcg
      601 aaaatcaaca attgcttgaa cagaattaaa ttaccagaga tgaaagaatt ctcatcaaga
      661 aaaaacatat gattacatcg atataatcat tgaagattat gaaaatatca aagaaaatgc
      721 ttatctggtc gtcccccaaa ttaattatac tatggattta aacatagaag actccagctc
      781 agaagagtta ataccctcga gaaagacgaa aattcttcgg acaatggctt tgaagtcgaa
      841 gaatataacg catatgaagc atataactca gagaatctat attttaccag agaggaatat
      901 acgtatgatt acgacctttc aaatgcaata tagacttaat aacttcttac aaaatctata
      961 attaataccg tagatattca ttcatcttac cctgtgcacc aaaagcaaaa actcactgac
     1021 gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta
     1081 cgccatctct gcaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc
     1141 cgcatccggc atgaataaag cgcactcaac aatctgaaac ccgccggaac ggtttattac
     1201 gtacatcagg tgaaactgac cgataagccg ctttcttttg ggtatagtgt cgtggacagt
     1261 cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt tacgtaaatc
     1321 aggtgaaact gaccgataag ccgggttctg tcgtggacag tcattcatct aggccagcaa
     1381 tcgctcactg gctcaagcag cctacccggg ttcagtacgg gccgtacctt atgaacccct
     1441 atttggcctt gctccgggtg gagtttaccg tgccacggac tgttaccagc cgcgcggtgc
     1501 gctcttaccg caccctttca cccttacctg atcccgcttg cgcgggccat cggcggtttg
     1561 ctctctgttg cactggtcgt gggtttcccc cccaggcgtt acctggcacc ctgccctatg
     1621 gagcccggac tttcctcccc tccgcccgtc tcccccgaag aggacgacga cgaagcggcg
     1681 actgtctggt cagcttcggc gcgcagtata gagggtttgc gcgcccttgt caccccgcgt
     1741 tgcgcattcc aatcgccagt gtcgcggcga tattacgtga agcacggcag atattgtcat
     1801 aagccccgcg gaatgcttcg tccaacgtac ctatgctggt caatacgctg aagaccgcat
     1861 caatgccatg ctgatgtaca acgccaacat catcggtcag gctacccgca atgccaatca
     1921 ccggtttatg gtacttcttc gccacgtttg cgacaccaat cggtaccttg ccgtgaatac
     1981 tctggctgtc aatacgccct tcaccggtga tcaccagcgt acaatcgtga atatgttctt
     2041 ccagattcag cgccgtagtg acgatttcaa taccactttt cagttccgca ccaagaaacg
     2101 ccatcagcgc cgcgcccata ccgcccgcag ctcctgcgcc ggggacatct ttcacatcaa
     2161 catgcagcgc ttttttaatg acctcggcat agtgagagag gttattgtcc agctcaacaa
     2221 tcatcgcttc actggctccc ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca
     2281 ccagcggatt ggtgacatca caagcgacgc gaatgacgca atcttttaag cgcggatcga
     2341 ggccggaaat atcaatatca ttcagagtat taagactacc gccgccaaaa ccaatttcat
     2401 tgccgttggc gtcgcataat ttcgccccca gcgcctgtac catgcctgcg ccgccatcat
     2461 ttgtagcgct gccgccaatg ccgataataa tgtttgtcgc accgctctcc agcgcctgca
     2521 ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta
     2581 ccagctccag cccactggcc gccgccattt caataaacgc ggttttgcca tcgccggaga
     2641 tcccccaact ggcattcact ttctcgccca acggccctgt aacccaggcg tgacgttcgg
     2701 aaccctgggt ggctgcaatc atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa
     2761 cagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc tttttctatc gcctgcgcaa
     2821 cctcgctggc agataaactt tctttataag agtctggggc gattacgatt ttcataccta
     2881 tgcctgttac cacatgacgc cggagggcgt ttctcttatt cggcgtggat tccaggcccg
     2941 gattgcaata cgccatccgg gcacgacgtc attaacgagt aacttcgact ttcgccagtt
     3001 tttcgtagta gcacgccaga gcgctatgat ccgccgttcc taaaccatct gcacgcagtg
     3061 cctgcatcat ctccataacc gcagctgtga gcggcagttg cgcgccgacg ccgtgagaag
     3121 tatccagcgc attcgccaga tccttaatat gcagatcaat gcggaagccc ggcttgaagt
     3181 tgcggtccat caccatcggc gctttggcat ccagcacggt actgcccgcc agcccaccgc
     3241 gaattgcctg ataaaccagg tctgggttaa cgcccgcttt agttgccagc gttaacgctt
     3301 ctgacatcgc agcaatattc agcgccacaa tgacctgatt tgccagtttg gtgacgttac
     3361 ctgcaccgat ttccccggta tgcaccacgg aacccgccat cgctttcatc aaatcatagt
     3421 atttgtcgaa aatagccttg tcgccgccca ccatcactga cagcgtaccg tcgatggctt
     3481 tcggttcacc gccgctcacc ggagcatcca gcatatcaat gcctttcgct ttcagcgctt
     3541 cgctgatttc acggcttgcc agcggtgcga tagaactcat atcgatcaat accgtacctg
     3601 gcttcgcgcc ttcaataatg ccattctcac ccagcgccac ctctttcaca tgaggggagt
     3661 ttggcagcat ggttatgatg acgtcgcact gttcagcgat cgctttagcc gtagacgctg
     3721 tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg gttacggtca gcaaccacca
     3781 gcgagtaacc tgctttcaga aggtttttac tcattggttt acccataatc cccaggccaa
     3841 taaaaccaac tttcatagtc atatcaatca tctctcttgt tgcggtggtg gttatttttt
     3901 aaaggtatca gccagtttct gagtggcaga gcggaagacg ccaagatcgc tgccgacagc
     3961 cacaaacgtc gcgccccatt ccagataacg acgcgcatcg gcttcgaccg gcgcgagaat
     4021 accgctgggt ttgccgtgcg cactggcacg gttaaaaata tgctgaattg ctttttgtac
     4081 atccggatgc gaggcattgc cgagatggcc caatgccgca gccagatcgc tggggccgac
     4141 gaagataccg tctacacctt cggtagcggc aatagcatcg acgttatcta cgccctgctg
     4201 actttctatc tggaccagaa tagtgatgtt cttgttcgac tgagcgaaat aatccgccac
     4261 ggtgccaaac atattggcgc ggtgagaaac ggagacgccg cgaatgcctt ccggcgggta
     4321 acgagttgat gccaccgcct gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa
     4381 attatagaaa ccgatatcca gaagacgctt aataattacc ggctcgttgg tcggcactcg
     4441 cactactggc gcgctggcgc tgcctttcag ggccattaac tgcggaataa acgtggaaat
     4501 atcgtttggc gcatgttcgc catccagcac cagccagtca aacccagcca aaccaagaac
     4561 ttcagtgcta atcgggttag agagtgctga ccagcaacca atttgtacct gtttcgcagc
     4621 cagtgcggct ttgaatttat tcgggaaaac atcgttattc atcgcttata cctttgctta
     4681 tttctgcaat tccatacgtt taatgtcgcc aactacgaag aggtagcaga ccatcgccat
     4741 cagcgctgaa catcccacga aaaccagtgc tgcattgaag gagtgcagtt cacttaccag
     4801 gtagccaatc accagtggag tgacaatgga ggcaacattg ccaaagacgt taaagacgcc
     4861 gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa atcaccggcc agcccagagc
     4921 accaaatcct ttgccaaaaa aagccagcgc catcagcatg accaccagcg tggtgttgtt
     4981 ggtgtagtta cataagatga tggtggaagc cagcaacatt cccagcacaa tcggtagctt
     5041 acgtgccagg gtcagggata aaccgcgttt gatcagataa tccgagaaga cacctcccag
     5101 cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg accagaccca ctttcagaat
     5161 cgacatgcct ttttcctgca ccagataaat cgggaaccag gtgaggaaga accaggtgat
     5221 ggtgttgata aaatattgtc cgaaaaatac gcccaacatc atgcggttag agagcaattg
     5281 cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg cccggctttt tgtggtccat
     5341 atcgaccacc gcgccatttt cagagataaa cttcagctct tccgcagaca tacgtgggtg
     5401 atctgtcggg ttataaatca acttgatcca cagcgccgtc agcacaaaac caatcacccc
     5461 cataacggtg aagacgtgct cccagcccca ggcgaaagtc agccagccaa gcagaggcga
     5521 aaagagcgcc agcgagaaat attgcgccga gttaaagatg gcggaggcag taccacgttc
     5581 tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc gggaatgatg gcgcttccga
     5641 gaagccgagc ataaagcgca taaagaacat agagatccct gcccaggcca gcgggaacat
     5701 atcaacaaag ccttgcagga aggtgaatag cgaccagaaa aagaggctgt aggtgtaaac
     5761 ttttttcgag ccaaacttat caagcagcca gccgccggga atttgcatca gcaggtaggc
     5821 ccagccaaaa gcggagaaga tgtaa
//
LOCUS       DAJVTY010000202         5748 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVTY010000202 DAJVTY010000000
VERSION     DAJVTY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.203"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="OQQ66_003740"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="OQQ66_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1906486.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENSYRQMQMNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="OQQ66_003741"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="OQQ66_003741"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1906487.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="pgeF"
                     /locus_tag="OQQ66_003742"
     CDS             1885..2616
                     /gene="pgeF"
                     /locus_tag="OQQ66_003742"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1906488.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="OQQ66_003743"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="OQQ66_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1906489.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1446 a         1364 c         1550 g         1388 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcatctgca tctgacggta tgaattttcc
      121 atcagtggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 acgcggttaa cgacggcaac ccatgcacca cgttctgtat agtactcggc aacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggttgcatc ggtagtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aatgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg tggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggtaaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg ttgccatcaa cgctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atcccgctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accgcgcgac ctggtggtac atcctggcgc gggtaacctg gatggcacgg tactgaatgc
     1261 gttgcttcat tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct
     1321 ggataaagac accactggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaaaggc gtgaaattac tcgtgagtat gaagcggtgg caattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc aatcagccgc cacccgacca aacgtactca
     1501 catggcggtg catcccatgg gcaaaccggc ggtcactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaaccgga cgtacgcacc agatccgcgt
     1621 gcatatggcg catattaccc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcgtttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca actatgctgc gtctttatca cccgatctcc ggcatcgaaa tggaatggca
     1801 tgcgcctatt ccagaagata tggtggagtt gattgaggtt atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctctacccgt atcggcggcg tgagcttgcc accttacgac
     1981 tctctcaacc ttggcgctca ttgtggtgat aacccggagc acgtagagga gaatcgcaag
     2041 caactttttg ctgcgggaaa tttgccttca aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagctcac tggcgaacct tatgcctcaa aacgggcgga tgcctcttat
     2161 agcaatacgc ccggcacggt ttgcgcagtg atgactgccg actgcctccc tgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gttcatgctg gctggcgtgg actgtgcgca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataaac ctgaaaacat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttc gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgctaaagc aagtgcagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc agcttgcccg gcagcgtctg gcgaacgtgg gtgttgagca aattttcggc
     2521 ggcgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgattcggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgagg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcag caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat
     3781 accattgcga ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcagcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccggata aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagaa
     4141 aaggcatcgc tttctggtac gcagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgacgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgtcattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgc
     4501 cgtagccgtg cggggttggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaacgggtg tggggaaaac tgagctttgt aaggcgctgg cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaaca ttctgttgca ggtactggat gatggtcgtc tgactgacgg gcaggggaga
     4861 acggtcgact tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta
     4981 agccataact tccgtccgga attcattaac cgtattgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc ctcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccagtcta tggtgcacgt cctttgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agtgattcgc
     5281 ctggaagtta atgaagaccg gattgtcgct gtccagtaat gataaaacga gcccttcggg
     5341 gctcgttttt gtctataagt tagacggaaa agactatatt taagatgttt tgcctgaaaa
     5401 atgagcgaac gataaagttt ttatattttt cgcttgtcag gccggaataa ctccctataa
     5461 tgcgccacca ctgacacgga acaacggcaa acacgccgcc gggtcagcgg ggttctcctg
     5521 agaatctcaa cagagaaaag caaaaaaatg cttgactctg tagcggggaa gcgtattatg
     5581 tacaccccgc gcagctgaga aaaagcgaag cggcactgct ctttaacaat ttatcagaca
     5641 atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgaaaa atgaatacca
     5701 agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgag
//
LOCUS       DAJVTY010000203         5675 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVTY010000203 DAJVTY010000000
VERSION     DAJVTY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.204"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(136..2055)
                     /gene="dhaR"
                     /locus_tag="OQQ66_003744"
     CDS             complement(136..2055)
                     /gene="dhaR"
                     /locus_tag="OQQ66_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1906490.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLEATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            2283..3392
                     /gene="dhaK"
                     /locus_tag="OQQ66_003745"
     CDS             2283..3392
                     /gene="dhaK"
                     /locus_tag="OQQ66_003745"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1906491.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            3364..3996
                     /gene="dhaL"
                     /locus_tag="OQQ66_003746"
     CDS             3364..3996
                     /gene="dhaL"
                     /locus_tag="OQQ66_003746"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1906492.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4007..5425
                     /gene="dhaM"
                     /locus_tag="OQQ66_003747"
     CDS             4007..5425
                     /gene="dhaM"
                     /locus_tag="OQQ66_003747"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1906493.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1428 a         1446 c         1404 g         1397 t
ORIGIN      
        1 aagcaagtca acggaggata tcgcttcagc ttttaagtgc gtgatagaca agcccttaaa
       61 cgatgctgaa aaatgttagc gtgcgccgga atgaaatata gatcactctg atgtattaga
      121 aatcgtttct gtctttcata cccggcgctt aaactgccct gcatcaatgc catgttgctt
      181 cattttccgc cacagcgtag tgcggccgat tccaagtaaa gccgacattt cctgaatgcg
      241 accgcctgtg acctgggctg cgttaataat tgcctctttt tcaacttccg caaatgacag
      301 actggtggaa aggcgggtgg cgctgacatc atctgttgcc tgctcggtaa acagatgttc
      361 cggcaaatca ctgacgcgaa tgcgcccatt atcactactc agagcaagat tctcgatgac
      421 gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa accaggcgag cgagggcatc
      481 gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga ctgcgtaatt tgttattcac
      541 cagcgccgga atgctgccac gccgcatacg cagaggcggg atggtaattt caaatgcatg
      601 cagcgcgtaa tacagctggc gactaaaacg attttgttcc accagcattg cgaggtccgc
      661 ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa cgccgcgcat ccagtcgcgt
      721 gataacaccc tgcttgataa cctgaagcaa agcagactgt aactccaccg ccagatattc
      781 aatcttttca agaaacagcg tgccgccgtg cgccagttcc agccgactca gacggccatt
      841 ttcattgtcc gtacgatcgc caccaataaa ttcttccgcc agtgccgcat caccatataa
      901 ctcacaattg acggcgatat aaggacctgc agcacgctcg ctttcattat gaattgcctg
      961 acttagcagt gccttgccca cgccctcttc tccacaaagc aggacaggaa agctactgcg
     1021 cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc ggatcgtcct gtggcatatg
     1081 agcaaaggta tggctgactt ttcctaattg actggtcatc aactgccgca tctgttccac
     1141 aggatggagc aacaaaataa agctggttcc ctgcgtttct attatcggtt taagggttat
     1201 caccgcatca ataaactgat gctgactttc aaaggttgct tctacgtgtt tgagcggatg
     1261 tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt aagagttcag tgattgcccg
     1321 tccctgactt gccgtcgctt caaggcgcaa gacccgcgcc gcctgggcat tgataaattg
     1381 caaattaccc tgctcgtccc agctaatcac gccatcatcc atactttcta acagggcatt
     1441 aagttgattt aaatgacggt tagtttcagc gagcaaactg tccgtcagca gtaaatttcc
     1501 gacctcgcgg gcgattgcca acgtcaacgg caaatcagct gcggtagttt gctcaaccgg
     1561 acacgccagc gctattgttc ccgtcaatcg gcccttgctg tcaaacaacg gcgttgcaca
     1621 aaaggcccag ttccagagtg cctgtttgaa atgttgatcg gccatcgttt tcacggcctg
     1681 accagagata gccgctaacg atagcgcaca agtaccaata attccctcgg cgcaatacgt
     1741 gccgtcatgg aatccaagtg cacttagctg ctgcaaggtt tgcggatcgc cattacggct
     1801 gagaatgcag gcggtttcat cgaggataaa cagcgcacac tctcgcggtg ccatatattc
     1861 ccaggcatct tccagcgcag cctgcccgag cgtcagcatc gctttcttac gccgataaat
     1921 agaagcaaat gtcacgccct gggcctgatg tggtacgctc catgtctccc gtttcatcag
     1981 cttattgcat cgctcccagg aggttgcaat taagggagat atgccccgac catcgttgtt
     2041 aaaagcgcca ctcatatccc gcatccctta tgacaaaagg tgttccggct cgcaatttta
     2101 agtattaata cacgtaaaag tttcaatatg gaacattcat taaagcatat gtttcactat
     2161 gaaacataat ttgggctaat tttcttttcc cttgcctgat gcacaatgga ttcatcggca
     2221 gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta ccgtaattgc tggagcaaaa
     2281 taatgaaaaa attgatcaat gatgtgcaag acgtactgga cgaacaactg gcaggactgg
     2341 cgaaagcgca tccatcgctg acattgcatc aggatccggt gtatgtcacc cgagctgatg
     2401 cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg cagcggacac gagccgatgc
     2461 actgtgggta tatcggtcag gggatgcttt cgggggcctg tccgggcgaa attttcacct
     2521 caccgacgcc cgataaaatc tttgaatgcg ccatgcaagt tgatggcggc gaaggtgtac
     2581 tgttgattat caaaaattac accggcgata ttcttaactt tgaaacagcg accgagttac
     2641 tgcacgatag cggcgtaaaa gtgaccactg tggtcattga tgacgacgtt gcggtaaaag
     2701 acagtcttta tactgccggg cgacgcggcg ttgccaacac cgtattaatt gaaaaactcg
     2761 taggcgcagc ggcggagcgt ggcgactcac tggacgcctg tgcggaactg gggcgtaagt
     2821 tgaataatca aggccactca ataggtatcg ctctcggtgc ctgtaccgtt cctgccgcgg
     2881 gcaaaccttc ttttaccctg gcggataatg agatggagtt tggcgtcggc attcatggtg
     2941 agccgggtat tgaccgccgc tccttctctt cccttgatca aaccgtcgat gaaatgttcg
     3001 acaccctgct gggaaatggc tcataccatc gcactttacg tttctgggat tatcaacaag
     3061 gcagttggca ggaagaacaa caaaccaaac aaccgctcca gtctggcgat cgggtgattg
     3121 cgctggttaa caatcttggc gcaactccgc tttctgagct gtacggcgtc tataaccgcc
     3181 tgaccacacg ttgccagcaa gcgggattga ctatcgaacg taatttaatt ggcgcgtact
     3241 gtacctcact ggatatgacc ggtttctcaa tcaccttact gaaagttgat gacgaaacgc
     3301 tggcactctg ggacgccccg gtccacaccc cggcccttaa ctgggataaa ttaggagaaa
     3361 gcaatgtcac tgagcagaac tcaaattgtt aactggctca ctcgttgtgg cgatattttc
     3421 agcaccgaga gcgagtatct taccggactg gatcgcgaaa ttggcgacgc tgaccacggg
     3481 ctaaatatga accgaggctt tagcaaagtg gtggaaaaac tccctgctat cgcagataaa
     3541 gatatcggtt tcattctcaa gaataccggt atgacgctgc tttccagcgt cggtggtgcc
     3601 agtggtccgc tgttcggtac cttctttatc cgcgccgcac aggcgaccca ggcacggcaa
     3661 agcctgacac tggaagagct ttatcagatg ttccgcgatg gcgcggacgg cgtaatcagt
     3721 cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg tgtgggtgcc ggtggtggaa
     3781 tcgttacgtc agtccagcga gcaaaatctc tctgttccgg tggcgctcga agctgccagt
     3841 agcatcgccg aatccgctgc acaaagtacg attacgatgc aagcccgcaa aggccgcgcc
     3901 agttatctcg gtgaacgcag tattggtcac caggatcccg gcgcgacctc ggtgatgttt
     3961 atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa ttggtgatgg taaacctggt
     4021 catagtttca catagcagcc gactgggaga aggtgtcggt gaattagccc gtcagatgtt
     4081 aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt gacgatccac aaaatcctat
     4141 tggtaccgat gccgtcaaag tgatggaggc catcgaatct gttgctgatg ccgaccatgt
     4201 gctggtcatg atggatatgg gtagcgcatt attgagtgct gaaactgcgc tggaattgct
     4261 ggctcccaag atcgccgcaa aagtacgttt gtgtgctgcg ccgttggtcg aaggtacact
     4321 ggcagcaacg gtcagcgcgg cctcgggagc ggatatcgac aaagttatct ttgacgctat
     4381 gcatgcgctg gaagccaaac gtgaacaact gggtttaccg tcctccgaca ctgaaatctc
     4441 tgacacatgt cctgcgtacg atgaagaagc ccgttctctg gcggtggtca taaaaaaccg
     4501 taacggcctg catgtacgtc cggcctcccg gctggtttat accttatcga catttaatgc
     4561 cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca gagagtatta accagattgc
     4621 gttactacaa gttcgctata acgatacgct gcgcctgatt gcgaaagggc cagaagctga
     4681 agaggcactg atcgctttcc gtcagctggc tgaagataac tttggtgaaa cggaggaagt
     4741 cgctccacct actctgcgtc ccgttccgcc tgtttcgggt aaagcctttt attatcaacc
     4801 agttttatat acggtacagg caaaatcaac cctgaccgtg gaagaagaac aagatcgatt
     4861 acgccaggct attgacttca cgttattaga tctgatgacg ttaacagcga aagcagaagc
     4921 cagcgggctt gactatattg ccgcaatctt ttctggtcac catacactgt tagatgatcc
     4981 ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa cattgcacgg cagaatatgc
     5041 ctggcagcaa gttcttaaag aacttagcca gcaataccag caactggatg atgaatatct
     5101 acaagctcgc tatattgatg tggacgatct tctgcatcgc accctggtcc acctgaccca
     5161 aacgaaagaa gaactcccgc agtttaactc gccaactatt ctactggcgg agaacattta
     5221 tccttccaca gtactgcaac tggatccggc ggttgtaaaa ggtatctgcc ttagcgccgg
     5281 aagtccggta tcccacagcg ccctaatcgc ccgtgaactg gggattggct ggatttgcca
     5341 gcagggtgag aaactgtatg cgatacaacc agaagaaacg ctaacgctgg acgttaaaac
     5401 gcaacgtttc aaccgtcagg gttaagccac tgttcggatg gcatcgttct gatgtcatcc
     5461 ggcaatttac tcagcactaa tggtttttaa ctacctgtta tatttttcac caatttatga
     5521 tgatttttct tcgctaatca cgaacccaac gccgctggct ggctaataaa cagacgttat
     5581 ttgatcactc tatttcgccg ttatttatcc ctttaattcc cttttctaaa atgcctgaca
     5641 gttcgcagaa tgagatttcg atcatgcagc tagtg
//
LOCUS       DAJVTY010000204         5561 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVTY010000204 DAJVTY010000000
VERSION     DAJVTY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.205"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..145)
                     /locus_tag="OQQ66_003748"
     CDS             complement(<1..145)
                     /locus_tag="OQQ66_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906494.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSD"
     gene            671..1366
                     /gene="minC"
                     /locus_tag="OQQ66_003749"
     CDS             671..1366
                     /gene="minC"
                     /locus_tag="OQQ66_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1906495.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1390..2202
                     /gene="minD"
                     /locus_tag="OQQ66_003750"
     CDS             1390..2202
                     /gene="minD"
                     /locus_tag="OQQ66_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1906496.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2206..2472
                     /gene="minE"
                     /locus_tag="OQQ66_003751"
     CDS             2206..2472
                     /gene="minE"
                     /locus_tag="OQQ66_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1906497.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2951..3070)
                     /locus_tag="OQQ66_003752"
     CDS             complement(2951..3070)
                     /locus_tag="OQQ66_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1906498.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3031..3216)
                     /locus_tag="OQQ66_003753"
     CDS             complement(3031..3216)
                     /locus_tag="OQQ66_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906499.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3317..3490
                     /locus_tag="OQQ66_003754"
     CDS             3317..3490
                     /locus_tag="OQQ66_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906500.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3492..3836
                     /locus_tag="OQQ66_003755"
     CDS             3492..3836
                     /locus_tag="OQQ66_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1906501.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3840..4175
                     /locus_tag="OQQ66_003756"
     CDS             3840..4175
                     /locus_tag="OQQ66_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1906502.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4232..5561)
                     /locus_tag="OQQ66_003757"
                     /pseudo
     CDS             complement(4232..5561)
                     /locus_tag="OQQ66_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1516 a         1301 c         1298 g         1446 t
ORIGIN      
        1 catctgagtt cacacaaata tacgggtcgc aaacaacgcc gcacgccgga gattgaagtc
       61 cgccagcgtc atgtccagac gccattgatg agaacatcat ccccacacct gacaatccga
      121 tataaaaaat acgcatcata ttcatagcac atcctgataa tccaaccgta caacacttat
      181 gctcatttca tcatcaatcc gtaatcaaaa ccgctgacct ggacgagtta cgatgacctg
      241 acagaagtat cagacgtctt ctgcggtttc tatgattatg atagagcaat tctatggcgg
      301 atattaagat aacgggtatc tgttctttaa tgatgctgca aaaaatggaa agttaagaaa
      361 gcctggcgtg gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctggaga
      421 tgctgtcacg attagggtgc ggaaaaagga agcgtaaata agaacaatca aaagtacgac
      481 ggcaatgggt tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag
      541 aatagacttt tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata
      601 acatgcctta tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag
      661 taaggccagg atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt
      721 ggttcatctg catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc
      781 tcaggccccc gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga
      841 cccggtaaac tggtcagcga tgcataaggc ggtgtcggca accggtttgc gggttattgg
      901 cgtaagtggc tgcaaagatg cgcaacttaa agccgaaatt gaaaagatgg ggctgcctat
      961 cctgacggaa ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg ctccagcgca
     1021 aaatacaacg ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg
     1081 tatttatgct ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt
     1141 gattgccgat gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc
     1201 aagtggtgac cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat
     1261 cgcaggtgaa tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg
     1321 actgcagtta gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa
     1381 ggaatttcta tggcacgcat tattgttgtt acttcgggca aagggggggt tggtaagaca
     1441 acctccagcg cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata
     1501 gattttgata tcggcctgcg taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt
     1561 tacgatttcg tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat
     1621 aagcgtactg aaaatctcta tattcttccg gcatcgcaaa cacgcgataa agatgccctc
     1681 acccgtgaag gggtcgccaa agttcttgat gatctgaaag cgatggattt tgaatttatc
     1741 gtttgtgact ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac
     1801 gaagccatta ttaccaccaa cccggaagtc tcctcagtac gcgattctga ccgtatttta
     1861 ggcattctgg cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac
     1921 ctgctgttaa cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa
     1981 gatgtgctgg agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagtg
     2041 ttgcgcgcct ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa
     2101 gcctacgcag ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa
     2161 gaagagaaga aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat
     2221 ttctttctct cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt
     2281 gttgctgaac gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat
     2341 attcttgagg tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt
     2401 gagcaaaaag atggcgatat ttctattctt gagctgaacg tgaccttacc ggaagcagaa
     2461 gagctgaaat aagcccgctg taaaaacgca tttatcttca aggcagagtc atttctgcct
     2521 tgagtttttc atccctctca tccacgttgt ggtaaagcgg cgagtattct tgctgatact
     2581 cctcattgct atttccacct ccccctttac accttaaggc tgttcccacg cacagaacaa
     2641 aatgtttatg cctggttgag taaataacct cattgttagt gtggatacac tgggggatga
     2701 taaccccgca atttcattat tcattgttct cccataggat gaggtttccc gccgtaactg
     2761 gcgggctttt tttgccccag aattttccct tttagccccc tgtaaacgaa aactgcgccg
     2821 aagcgcagtt taatcgttga tggtcagaac gaccagttca cgatatatac tgcgggtatc
     2881 gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg acctaagctc
     2941 tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga gacagcgcag
     3001 gtgtattgtg ttcaccactc accaaagaca tcatgatgaa atgatgatat cccgcataag
     3061 aaagaggcat tttttaaacg cagtgcgctg aagtgtagtt ggataaaaaa tcactctttt
     3121 cagataatac tgtcgtacgc ttatctttcg acaatgaggc cgtcggcaaa ataaaatgac
     3181 taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca ataatattga
     3241 taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac ttcttgaata
     3301 aaaatattgc taatcaatga gttattcctc atttaagatt attctcattc acgtaaaaaa
     3361 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
     3421 tttagttaag cagatcttaa ttgaagacga aattattgtg cgtgcaactt acttaatacc
     3481 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
     3541 ccacgccagc gatggcggat atgaatcgca ccacgaaagg tgcattgcta ggcgcaggcg
     3601 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
     3661 gtgttggtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
     3721 taggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
     3781 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
     3841 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
     3901 cgcaggacta caatattaaa aacggtttac cgtcagaaac ctatattact tgcgcagaag
     3961 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
     4021 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
     4081 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
     4141 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc gcgtcgatga
     4201 atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg cccaccatca
     4261 ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc cagacgctgg
     4321 ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta ctcgccccat
     4381 cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta tggatccagt
     4441 tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac
     4501 gaataaaggt gcggaaacca agtcgggtct ggatgttgtt atttgcgtca ttctcaatac
     4561 gtgagccatt ggcttcggtg tgcgcattat gatcgacgcc catccagata gcctgtgcct
     4621 gtggctggaa tatccacgtg tatttcgccg cttccagacc aaaggattta ttcagggcaa
     4681 agccctaacc cgcttccagc gagacggttg cgcctttcgc actataggat tcataggcta
     4741 attcatcccc tttcaccgag tttttaaacc agctatattg cgcccaggcg tcaatgtatg
     4801 cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg caatagcctc
     4861 tgacactccc tttcgaacga taatccgaca cgctggaatg agttgagttg tagtcgcggg
     4921 cataaccagc catcactccc aaacgccaac tgtcactgtc ggtaaaacca ccggttaaca
     4981 gatcggcccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt
     5041 cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt
     5101 cagttacagg atcgatgtag cgcatttcac ccgcacggtc gttcagacgc ataacaaaaa
     5161 gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc aggacggggg
     5221 taggcgtttc tgttggttct gaatttccct ctgagctgtt atcctcctca ttcactgact
     5281 gataacttgt caaataccag tttttgcctt gttgttttag ttgatattcg aaagctccag
     5341 ctgcaatcct tgcatcacct tgtctgaagg cttctgctgg agattcatcg ccattcacag
     5401 ttatgagttc gatcccgttt agcgtggccg cgccgcttcc tccagcattt ttcacataga
     5461 cggtagttga acccgaagta ttaccctcaa caataagtct gtccgtagcc gatttatcat
     5521 cacctaatac ggtgcggata ttaagctgac cattattacc g
//
LOCUS       DAJVTY010000205         5552 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVTY010000205 DAJVTY010000000
VERSION     DAJVTY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.206"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(4..252)
                     /locus_tag="OQQ66_003758"
     CDS             complement(4..252)
                     /locus_tag="OQQ66_003758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906503.1"
                     /translation="METITHPTLVQLVAAGAVRVVIAVGQPGGWTLLVRYGLAERALA
                     AQRSKQLRVFRKLDTLVLYLQQIGVCRFEVNRPGNPGD"
     gene            complement(440..538)
                     /locus_tag="OQQ66_003759"
     CDS             complement(440..538)
                     /locus_tag="OQQ66_003759"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906504.1"
                     /translation="MATIIVHCPRCQSDCVCRHGKGAVSVPCWRVF"
     gene            complement(580..1599)
                     /locus_tag="OQQ66_003760"
     CDS             complement(580..1599)
                     /locus_tag="OQQ66_003760"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1906505.1"
                     /translation="MLYSIQFLRGIAALLVVMAHTAHKGNIYNTETLSWFHIGGSGVD
                     LFFIISGFIMCYTTHNKNLTIISFLTLRVQRIIPLYWVFTTLALTVFVISPSLVNSSG
                     GETSIIDSYFLIPSSAKYLVNNGWTLSYEFYYYLIFSLFLVAFSSTKLRYACIIATLL
                     ILSSFGLIFQPEDIVVDFLLSSWLYEFAIGVILFVAYDRGLVKANFGYISVTLALAFL
                     LYKNFNMEYLSGAPRVISIGVPMGLLFYGFLSFEGYFKRKKHFISNLFERIGDSSYSL
                     YLSHPFVLSPMAIIASKLGVTNSFLFGVILIGSSVFIGYATYVIIEKRIIYFTKIKLV
                     MPSVG"
     gene            complement(1794..2057)
                     /locus_tag="OQQ66_003761"
     CDS             complement(1794..2057)
                     /locus_tag="OQQ66_003761"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1906506.1"
                     /translation="MDTHYYVSSLEIAPEQAAKAVRQHWHIENQQHWVLDVAYREDAS
                     RLGDREAAGFFALFRRILRPSNRMSHLGKLWFRTHYSPNKKRL"
     gene            2323..2616
                     /locus_tag="OQQ66_003762"
     CDS             2323..2616
                     /locus_tag="OQQ66_003762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906507.1"
                     /translation="MIGILKESPKDPDSLKVISITTPARGFSKYGFGSTEHGWFTTIT
                     YNAKNSYGGYVGAKSITFVYLNGDYKVAVPMSNGERVMLDKVSFSCKDNCQTK"
     gene            complement(2644..2799)
                     /locus_tag="OQQ66_003763"
     CDS             complement(2644..2799)
                     /locus_tag="OQQ66_003763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906508.1"
                     /translation="MNSKQKCSHSNLEKEYYLGSATGDYKCLDCGEVVFGKDWPEKER
                     SKNDNEE"
     gene            3762..3911
                     /locus_tag="OQQ66_003764"
     CDS             3762..3911
                     /locus_tag="OQQ66_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906509.1"
                     /translation="MPFEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            4981..5205
                     /locus_tag="OQQ66_003765"
     CDS             4981..5205
                     /locus_tag="OQQ66_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HCS1906510.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYY
                     LSAETAARVRRGDEAVHSSEDVRKSLGLDD"
     gene            5189..5455
                     /locus_tag="OQQ66_003766"
     CDS             5189..5455
                     /locus_tag="OQQ66_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1906511.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSG
                     KPLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
BASE COUNT         1547 a         1265 c         1225 g         1515 t
ORIGIN      
        1 agtttagtct ccaggattcc cggggcggtt cacctcgaac cggcaaaccc cgatctgctg
       61 caggtagaga accagggtat cgagcttgcg aaaaactctg agctgcttgc tgcgctgggc
      121 ggccagggca cgttcggcta gaccgtagcg caccagcagc gtccagccgc caggttggcc
      181 aactgcgatc acgacacgaa cagcaccggc cgccaccagt tgtaccaggg tgggatgggt
      241 aatggtttcc atgggggaga ttccgagaca agggatagtg cggcgcagga tgggagataa
      301 cgcctagcaa aacaagtcat cttgcaggat gaccagcagc aaaaagggcg tccgatttaa
      361 cgtaacgccc ccttatgcgc agtcatccag gcgggttatg actggggtgg gtactgagca
      421 acgtagctgg ttgtcagctt caaaagactc gccaacacgg cacggaaaca gcaccctttc
      481 cgtggcggca gacgcagtct gattggcaac gtgggcaatg aacgatgata gtggccataa
      541 ggaatactga gtcttaaaag ccaccatggc tcacgttcat caacctactg aaggcatcac
      601 caatttaatt ttggtgaaat aaattattct tttctcgata attacgtaag ttgcataacc
      661 aataaatacg gacgacccaa ttaatataac cccaaaaaga aatgaatttg tcacccctaa
      721 cttactagca ataattgcca tcggacttaa aacgaaagga tgggaaaggt ataaggagta
      781 agagctatct ccaatccgtt caaataaatt tgagataaaa tgtttttttc gtttaaaata
      841 cccctcaaat gataagaaac cataaaacag caaccccatt ggaacaccaa tagatatcac
      901 tcttggagca cctgataaat actccatgtt aaagttttta tatagcaaaa aagccaatgc
      961 caatgtaact gagatataac caaaattagc tttcaccaac cccctgtcat aagcaacaaa
     1021 taatataaca ccaatcgcaa actcatacag ccaactgctt aacaaaaaat ccaccactat
     1081 atcttctggc tgaaaaatca atccaaaaga ggataaaatt aagagagtag caatgataca
     1141 tgcatatctt agtttggttg acgagaaagc aaccaaaaat aacgaaaaga ttagatagta
     1201 ataaaattca tagcttaatg tccatccgtt atttaccaag tatttggcac tagatggtat
     1261 taaaaaatac gaatctatga tgctagtttc tccaccagat gagttaacaa ggctgggaga
     1321 tataacgaac acagtcagag ctagagtcgt aaatacccag taaagaggaa tgattctctg
     1381 aacacgcaac gtcaaaaaac ttatgattgt taaattttta ttatgtgttg tgtagcacat
     1441 tataaaacct gaaataataa aaaaaagatc gacccccgac ccgcctatat gaaaccaact
     1501 taaagtttct gtattgtaaa tatttccttt atgagcagta tgagccataa cgacaagaag
     1561 agcagcaata ccccgcaaaa actgtataga atataacatc gaccacctaa ttccttacat
     1621 tatagttcat ataacctaac catcttaagc aagtgttaga aatgggtact taatggacac
     1681 caaaaaaatt taagctttcc aataagttag ctagctaaag gccatgattg gcccaataat
     1741 agtattctac caataaacca ccagttaata caccgccaac ggcacccgct gttttataaa
     1801 cgcttcttgt ttggcgagta atgagttctg aaccagagct tacccagatg cgacatccta
     1861 tttgaggggc gcaggatacg gcgaaacagg gcaaaaaacc ctgcagcttc tctatctccg
     1921 agccgactgg catcttcccg atacgccaca tccagcaccc aatgttgctg attttcaatg
     1981 tgccaatgct ggcgaaccgc tttggctgcc tgttccggcg ctatctccag cgagctgacg
     2041 taataatggg tatccaagct tagagcctat cccaataggc tcttaaagtc ggcaacgtct
     2101 atttattttc acagcgcata acggatgtta tggtaaatct atctataaat atctattata
     2161 gtggatttcc tatagttgtt ttggaaaatg acaaggatat atatgaaaat aattaagtgg
     2221 gcttcactgt ctggtgttct gttaatatct gcttgtggtg tttcttagaa cgaagtaaat
     2281 agtgctaatt atggtttaaa accaatagat aatgatgcga aagtgattgg tatacttaaa
     2341 gagagtccaa aagatcctga ttctctaaaa gttatatcaa taaccacccc cgccagaggt
     2401 ttttctaagt atggctttgg ctctacagag catggctggt tcactactat cacatataat
     2461 gcaaaaaact cttatggcgg ttatgttggt gctaagtcca ttactttcgt ttacttaaac
     2521 ggagattata aagtggcagt gccaatgtct aatggtgaga gggttatgtt agataaagtg
     2581 tcattttcat gtaaagacaa ttgccaaact aaatgataaa aaaggtcgca atagcggctt
     2641 tttttattct tcgttatcat ttttacttct ttctttttct ggccagtcct tcccaaaaac
     2701 aacctccccg caatccaaac atttataatc tcctgtggcg ctacccaagt agtattcttt
     2761 ttctagattt gaatgagaac atttttgttt gctgttcact acagtcacct ttggatattc
     2821 atttattcgt gaatgatttt tatctatacc gacattgtta aatgatggaa agatcttctt
     2881 gagatccttt ttttctacgc gtaatcttca tgactgtaaa cgaaaaaacc accctggcag
     2941 gtggtttttc gaaggttagt taatcctggc agattctcta accgtggtaa cagtcttgtg
     3001 cgagacatgt caccaaatct gtccttccag tgtagcctca gtcaggccgc cacttcaaga
     3061 actctgaaca catctctcgc acaaacctgt taccagtggc tgctgccagt ggcgttttgt
     3121 cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cagtcggact
     3181 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     3241 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     3301 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
     3361 cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt tgtgatgctt gtcagggggg
     3421 cggagcctat ggaaaaacgg cagcgccgca tcctttttcc ggccttccct gacttgagtg
     3481 ccatctcttt atcttgtcat tcgtgagcag gtaacgcccg ccgcagcctc acggcaggag
     3541 cgtagcgacc gagtgagcga ggaggcgtca tatcaccgta actcccattt tctcccttac
     3601 gccctgtagc tgcattttcc cctgtcacat gaagcacttt cattaggtgc tactccgccg
     3661 taacatattc agccactgta cactccgcta gcgctacgtg actggttcag tgctgcgctc
     3721 cgaaacccgc taacaacctc tccgctacta gatatcaccc catgccattt gaatatgatg
     3781 aacaactgga ttcgactgat acgcagcttc ccggcgaata tgaccttttt gatttaatgg
     3841 cgcaggttac tgaagagaac ctgcacgccc cggtggattt tgggccgcct gtcggccggg
     3901 aagcgttata gctttttcgc ttacccgcgt acgtgtaata cgccggtatt acatcttatt
     3961 tctttatttt catgtgatta caggtggttg taatacgttg cgtattacat aaataaacgg
     4021 gaacttcagt ttttaccttt taattcatgc aattgcgatg aaaatcgcgg ttgcgtatgg
     4081 tgtatggcca tttaagggat aaagaatcat ctccttaccg ggctgagctt tgcctattct
     4141 gaggagatct gtgaaaaaaa gcgtccataa ctagctcgtt catacttgta tgaacagctt
     4201 attcctcctg actactaata aactacagcg tcactactga tttactacaa atcggaggag
     4261 gatgactact tatctactac tgatttacta ctgaattact actgaattac gtcataacag
     4321 ttctctgaac cattcgtaca tccgaccccc ttctcttcat gcttgatgct cttgccaacg
     4381 cttcacggtc acgcaataaa ttgcgtcccc gttcaccgtt ttcggatgag aagaacgtcg
     4441 aaaacaggac ttccactggc tgcacggcgt gaaattctgc gctatgcact tgcgcgcata
     4501 ctcatgcatg ccgtaaaaac agagcctgcg tgtttctggc gggttttcgg gtggtttgtt
     4561 gccttgtttt accggtttcc cgtcagaaac gctctcagag cctctcaggc ggtgcacgca
     4621 acagatgtta tgttcaatca gactgatctg cgggtacaaa aaaagccccg ggggctttta
     4681 actactgact tatttttttg aggatttgct gggtttcaat ttgtcagtat tccccggcgt
     4741 ttttttattg tcacgctggc gtttatcagg ttcccctgtt gaatcggcaa ttcgtttcgg
     4801 acccggctta agacctataa tcgttatcct ttcaatagtt aagaggaaag ccctcaagct
     4861 ggatatattg acagcgttac ggattatcaa gaggggccag caagattatt ttttgttctt
     4921 ttaggttgat tgcgatacta ttgctataca tgtatatcaa atggtgttca ggaggtttat
     4981 atgcttgcta tccggttatc tgatgagatt gagtcccgtc tggactcgct ggcgaagcaa
     5041 accggcagaa caaagacgtt ctatgcgcgg gaagcaatac tggcgcatct ggaagacctg
     5101 gaagattatt atctttcagc agaaactgcc gcacgcgttc gccgtggtga tgaagccgtg
     5161 cattcgtctg aagacgtgag gaagtcactt ggtctggacg attaactatt ccgaccgggc
     5221 gctcaaatcg ttacgcaaga tggacaaaca gaacgcacga cggattgtgg attttatgga
     5281 tttacgcatt gcagttgctg ccgatcctcg ccagtcaggg aagccgctca aaggtgagct
     5341 gggcgagttc tggcgctatc gggtgggaga ttatcgcatt ctgtgtgaga tccgggatga
     5401 cgagctggtt atccttgccg ccaccattgg ccatcgccgc gaagtttacg actgaagccc
     5461 gctatgcggg ctttttttga tgctgataac gccttttatg ttcaagagct cctgatctgt
     5521 ctcggggcac ccatgaagcc cgtggcatcc ac
//
LOCUS       DAJVTY010000206         5545 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVTY010000206 DAJVTY010000000
VERSION     DAJVTY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.207"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1138
                     /locus_tag="OQQ66_003767"
     CDS             <1..1138
                     /locus_tag="OQQ66_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1906512.1"
                     /translation="LAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNP
                     HQPKTATGVIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQW
                     IEDNVTFPSTMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRP
                     EWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYG
                     LMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRW
                     HLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQS
                     LLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1174..1377)
                     /gene="ydfZ"
                     /locus_tag="OQQ66_003768"
     CDS             complement(1174..1377)
                     /gene="ydfZ"
                     /locus_tag="OQQ66_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1906513.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1374..2240)
                     /locus_tag="OQQ66_003769"
     CDS             complement(1374..2240)
                     /locus_tag="OQQ66_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1906514.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIEHKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVARSA
                     RKTATGLAKSNSFTLIPSGMRGFSPRLTAHACIKKDVSLIKNKKYFTRQEYLYCARYV
                     GFDCKSERRKHL"
     gene            complement(2329..3075)
                     /gene="ydfG"
                     /locus_tag="OQQ66_003770"
     CDS             complement(2329..3075)
                     /gene="ydfG"
                     /locus_tag="OQQ66_003770"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1906515.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3212..5257
                     /gene="dcp"
                     /locus_tag="OQQ66_003771"
     CDS             3212..5257
                     /gene="dcp"
                     /locus_tag="OQQ66_003771"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1906516.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5302..>5545)
                     /locus_tag="OQQ66_003772"
     CDS             complement(5302..>5545)
                     /locus_tag="OQQ66_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HCS1906517.1"
                     /translation="VAAEMATGAIERADADVSIAITGYGGLEGGEDGTPAGTVWFAWH
                     IKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1374 a         1345 c         1440 g         1386 t
ORIGIN      
        1 gttggctgcg atgtgtgaac cgcaaatcgc gattgtctct ctgacaatca ccgaaaaagg
       61 gtatttccac tctccggcga ccggacagtt aatgctcgat cacccgatgg tcgctgccga
      121 cgtgcaaaat ccccaccagc cgaaaacagc aacaggggtg attgttgagg cgctggcccg
      181 ccgtaaagcg gcaggacttc ccgcatttac cgtcatgtca tgtgacaaca tgccagaaaa
      241 cggtcatgtt atgcgtgacg ttgtcacttc atacgcgaaa gctgttgatg taaaactggc
      301 acaatggatc gaagataacg tgactttccc atcaacaatg gtggaccgta ttgtgcccgc
      361 agtgacagag gatacgctgg cgaaaatcga acaacttacc ggtgtgcgcg atcctgcggg
      421 cgttgcctgt gaacctttcc gccagtgggt aatagaagat aactttgttg ccggacgtcc
      481 agaatgggaa aaagcgggag ccgaactggt tagtgatgtg ctgccttatg aagagatgaa
      541 gttgcgcatg ctcaacggca gtcattcatt cctggcgtat ttgggttatc ttgccggata
      601 tcagcacatt aatgactgca tggaagatga acattatcgt tatgcggcgt atggcttgat
      661 gttgcaggaa caagcgccga cgctgaaagt gcagggcgtt gatttgcaag attacgctaa
      721 ccgattaatt gaacgctata gcaatccggc gttacgtcat cgaacctggc agattgcgat
      781 ggatggtagc cagaaattgc cacagcggat gttggattct gttcgctggc atctggcgca
      841 tgacagcaag ttcgatctgc tggcgctggg cgtcgcgggt tggatgcgtt atgtcggtgg
      901 tgttgatgaa cagggaaatc cgatagaaat cagtgaccca ctgttacctg ttattcagaa
      961 ggctgtacaa agtagtgccg aagggaaagc gcgcgtccag tcattgctgg cgattaaggc
     1021 aatctttggt gatgatttgc cagacaatag cttgtttact gcaaaagtga cggaagcgta
     1081 cttgtcttta ttagcgcatg gcgcgaaagc gaccgtggcg aaatattccg tgaagtaaaa
     1141 aaagcaatgc cgccatcggc ggcattcaca cacttagttc atgccgagac gaatcaggtc
     1201 cagaggtgcc agtttctctt cacaaccttc aacaactacg gtttttccgc ggcggatttg
     1261 ctcagcggtc agaccttcag tatcaatcga caggatcttg ccagtgtgac cggtgccgct
     1321 aaccataaca cggctgccag tggtgattgc gttacggtta cgatcgtagg tcgtcatagg
     1381 tgttttctcc tttctgattt acagtcgaag cccacgtaac gggcgcaata taaatactcc
     1441 tgtcgagtga aatatttttt gtttttgatc aagctcacat cttttttgat gcatgcatga
     1501 gccgtaaggc ggggggaaaa gcctctcatc ccggatggga tgagagtaaa tgaattactc
     1561 ttcgctaaac cagtcgctgt tttcctggcg gatctggcca cggattcgct gatttcctgc
     1621 aaatgttgcg tcattgcacg ttctaccgca tcgccatcac gtttttgcag ggcagagaaa
     1681 atatcaagat gctggcgtaa cagcatttct ggtggagaaa catggtcgaa actcatatag
     1741 cgcacgcgat caacggtcgc tttcaggttc tcaatggtat cccacgccag ttgacagtcg
     1801 gcaatctgcg tcaggagttg atggaagttg tcatcaagtt caaaaaaatc atccagttgc
     1861 ttgtgctcaa tggcaatgcg ttgctggtga agattttgtt ccagttggta gcactggctt
     1921 tcggtaatca tgctcgccgc ccgacgcgcc accgcgcact caatggcctg acggataaaa
     1981 ctgccgttgc gcacctgggc catggaaatt ttgttgacgt agctgccacg ctgtggacga
     2041 atttgaatca ggccgttttc cgccagttta ataaaggctt cacgaaccgg ctggcgtgac
     2101 acattgaaac gaacagaaac ttctttttcc gacaacggtg tgcctggagc aatcaggcaa
     2161 tggacaatgt cgcgacgaag gatacgataa atttgctgat tgacaggctg tgtgggatta
     2221 agttgcgttt cgacggtcat tcgttcttgc ttgtgagtga gttaactgca tgagtctacc
     2281 acttttttgt gacaagcaat aacccggcag ttacgccggg tataaaaatt actgacggtg
     2341 gacattcagt ccggcatagc tttgggtaac cggcatcatt tccagggtat tgatattgac
     2401 gtgagcaggc agcgttgaca cccaccaaac ggcttcgctg acatcttctg gcgtcaatgc
     2461 aacggtattt tgataggttt tttccgcttt accgtcatcg cctttaaagc ggacattgga
     2521 aaactcggtg ccacccacca gacccggttc gatgtcggtg acgcgcaccg ccgtaccatg
     2581 caggtccgta cgcagattca ggctaaactg acgaacaaac gctttcgtcg caccgtaaac
     2641 gttaccaccg gcataaggcc agctacctgc cgttgagcca atgttaataa tatgaccatg
     2701 attacgttca accatacccg gtaagacggc gcgcgtcata tataccaggc ctttgttgtt
     2761 ggtatcaatc atcgtttccc agtcttcaac gctggcttta tgcgcaggct ccatgcccaa
     2821 cgccaggccg gcattattta ccaggatatc aatattgcac cactcggcag gaagcgatgc
     2881 cagcatctct tcaatagcgg cgcggttgcg aacgtccagt tgggcgatgt acagattatc
     2941 tcccagttcg tcttttaact cctgcaaccg ttcctggcga cggccagtgg cgataacttt
     3001 atgcccttgt tgaataaaac gacgagtaat gcattcacca aaacctgccg ttgctccagt
     3061 tactaaaacg atcatctcac tgttcctcaa cgcttttgtg tgtcaataac atagcacgtg
     3121 atgcttaaga gcgggtaata ctggtttgta agattgcact ccgttatcag taagcctacg
     3181 ctatggaaca cctcgcgttc aggagaagaa aatgacaaca atgaatcctt tccttgtgca
     3241 aagcacacta ccgtatctgg ctccccattt tgatcaaatt gccaatcatc actatcgccc
     3301 ggcattcgat gagggaatgc agcaaaagcg ggcagaaatt gctgccatcg cgcttaaccc
     3361 gcaaacgcct gatttcaaca atactattct ggcactggaa caaagcggag aattacttac
     3421 ccgcgttacc agcgtctttt ttgcgatgac tgcggcgcat accaatgatg aattacagcg
     3481 tcttgatgaa cagttttccg ctgaactggc ggaactggct aatgatatct atctgaacgg
     3541 tgaattattc gcgcgggtag atgctgtctg gcagcgccgt gaatccctgg ggcttgatag
     3601 tgaatccatc cgcctggtgg aggtgattca tcaacgtttt gtccttgccg gagccaaact
     3661 tgcgcaagct gataaagcaa aattaaaagt actgaataca gaagctgcga ccctgaccag
     3721 ccagtttaac cagcgattac tggcagcaaa taaatccggc ggtctggttg tgaacgatat
     3781 cgcgcagctg gcaggaatga gtgagcaaga gattgcgctg gcggcagagg cggctcgcga
     3841 gaaaggtctg gataacaaat ggctgattcc gctgctgaat accacccaac aaccggcgct
     3901 tgccgagatg cgcgatcgtg cgacgcgtga aaaactgttt attgcgggct ggacgcgagc
     3961 ggaaaaaaat gatgccaatg atacccgcgc tatcattcaa cgtctggtag agattcgcgc
     4021 acagcaggcg aaactgcttg gttttcctca ttatgccgca tggaaaatcg ccgatcagat
     4081 ggcaaaaacg ccagaagcag cactcaactt tatgcgggaa attgttccag cggcgcgtca
     4141 acgtgctagc gatgaattag cctccataca ggcggttatc gataagcagc aaggcgggtt
     4201 tagcgcgcag ccgtgggact gggcatttta tgccgaacag gtacggcggg agaaatttga
     4261 tcttgatgag gcgcagctca agccatattt tgaattaaac acggtgttga atgaaggtgt
     4321 attctggacc gcgaatcagc tcttcggtat taagtttgtc gaacgttttg atattcctgt
     4381 ctaccatcct gacgttcgtg tgtgggaaat ttttgatcat aatggcgtgg gactggcgtt
     4441 attttacggt gatttcttcg cccgtgattc aaaaagcggc ggtgcatgga tgggcaattt
     4501 tgttgagcaa tcaacgctta atgaaacgca tccggtaatt tataacgttt gcaattatca
     4561 gaaacccgct gccggtgagc ctgcgttgtt actctgggat gatgtcataa ccttattcca
     4621 tgaatttggt catacgctgc acggcctttt tgcccgccag cgttatgcca cgctttccgg
     4681 caccaacacg ccgcgtgatt ttgtcgaatt tccgtcgcaa atcaacgaac actgggcaac
     4741 gcatccgcag gtattcgctc gctacgcccg gcattatcag agcggggcag caatgcctga
     4801 cgaactgcaa cagaaaatgc gtaatgccag cctgttcaac aaagggtatg agatgagcga
     4861 actgcttagc gccgcacttc tcgatatgcg ctggcattgc ctggaagaaa acgaagcaat
     4921 gcaggatgtc gatgattttg aattgcgggc gctggtggcg gaaaatatgg atcttcctgc
     4981 tataccgcca cgctatcgca gcagttattt cgcccatatt tttggtggcg gatatgctgc
     5041 aggttattac gcttatctgt ggacgcaaat gttggccgat gatgggtacc agtggtttgt
     5101 tgagcagggc ggattaacgc gtgaaaatgg gcagcgtttt cgcgaggcga tcctttccag
     5161 aggtaacagc gaagatctgg aacgcctgta tcgacaatgg cgcggtaagg ctcctcagat
     5221 tatgccgatg ctgcaacatc gtggcttgaa tatataaaca ccaccaatgg ctggcctgga
     5281 gatatcgcca ggccagcctg gttatagcag taattgcagc agctggacgt gggcaaacct
     5341 caccgctaaa gctaataccg tttcgcagtc gccagcaaaa tgcataaccg cagtgtaggt
     5401 ctggccttta atatgccacg cgaaccagac ggtacccgct ggcgtaccat cttcaccgcc
     5461 ctccagtccg ccgtagccgg taatggcaat actgacatca gcatccgcac gctctatggc
     5521 acctgttgcc atttctgctg ccact
//
LOCUS       DAJVTY010000207         5525 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVTY010000207 DAJVTY010000000
VERSION     DAJVTY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.208"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..585
                     /gene="pdeC"
                     /locus_tag="OQQ66_003773"
     CDS             <1..585
                     /gene="pdeC"
                     /locus_tag="OQQ66_003773"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1906518.1"
                     /translation="TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQ
                     KTEQYAVRPQQIKFEVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKS
                     LNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRG
                     VRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ"
     gene            complement(588..911)
                     /gene="soxS"
                     /locus_tag="OQQ66_003774"
     CDS             complement(588..911)
                     /gene="soxS"
                     /locus_tag="OQQ66_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1906519.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            997..1461
                     /gene="soxR"
                     /locus_tag="OQQ66_003775"
     CDS             997..1461
                     /gene="soxR"
                     /locus_tag="OQQ66_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1906520.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2007..3356
                     /gene="ghxP"
                     /locus_tag="OQQ66_003776"
                     /gene_synonym="yjcD"
     CDS             2007..3356
                     /gene="ghxP"
                     /locus_tag="OQQ66_003776"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1906521.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3508..5157
                     /locus_tag="OQQ66_003777"
     CDS             3508..5157
                     /locus_tag="OQQ66_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1906522.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5311..>5525)
                     /locus_tag="OQQ66_003778"
     CDS             complement(5311..>5525)
                     /locus_tag="OQQ66_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1906523.1"
                     /translation="RVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSH
                     TGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1171 a         1319 c         1534 g         1501 t
ORIGIN      
        1 actgattatg ttattgataa tgtcttccgc gatctgggcg attacctggc aacacatgca
       61 gatcgctatg tttctattaa cctgtcggcc tccgattttc atacgtcacg gttgatagcg
      121 cgaatcaatc agaaaacaga gcaatacgcg gtgcgtccgc agcaaattaa atttgaagtg
      181 actgagcatg catttcttga tgtcgaaaaa atgacgccga ttattctggc tttccgccag
      241 gctggttacg aagtggcaat tgatgatttt ggtattggct actctaactt gcataacctt
      301 aaatcattga atgtcgatat tttgaaaatc gataaatcgt ttgttgaaac gctgaccacc
      361 cacaaaacca gtcatttgat tgcggaacac atcatcgagc tggcgcacag cctggggtta
      421 aaaacgatcg ctgaaggcgt cgaaactgag gagcaggtta actggctgcg caaacgcggc
      481 gtgcgctatt gccagggatg gttttttgcg aaggcgatgc cgccgcaggt gtttatgcaa
      541 tggatggagc aattacccgc gcgggagtta acgcgcgggc aataaaacta caggcggtgg
      601 cgataatcgc tgggagtgcg atcaaactgc cgacggaaaa cgcgggagaa ggtctgctgc
      661 gagacataac ccaggtccat tgcgatatca aaaatcggac gctcggtggt gcgcaactca
      721 acggcggcca gtaacaggcg acgttggcga atgtaatcgc caagcgtctg atgcgtcacc
      781 gtgcggaaca ttcgttgcaa gtaccacttt gaatagcctg atttttttgc gactacatca
      841 atgttaagcg gctggtcaat atgctcgtca atccatgcga taagatcctg aataattttc
      901 tgatgggaca taaatctgcc tcttttcagt gttcagttcg ttaattcatc tgttggggag
      961 tataattcct caagttaact tgaggtaaag cgatttatgg aaaagaaatt accccgcatt
     1021 aaagcgctgc taacccccgg cgaagtggcg aaacgcagcg gtgtggcggt atcggcgctg
     1081 catttctatg aaagtaaagg gttgattacc agtatccgta acagcggcaa tcagcggcga
     1141 tatagacgtg atgtgttgcg atatgttgca attatcaaaa ttgctcagcg tattggcatt
     1201 ccgctggcga ccattggtga agcgtttggc gtgttgccag aagggcatac gttaagtgcg
     1261 aaagagtgga aacagctttc gtcccaatgg cgagaagagt tggatcggcg cattcatacc
     1321 ttagtggcgc tgcgtgacga actggacgga tgtattggtt gtggctgcct ttcgcgcagt
     1381 gattgcccgt tgcgtaaccc gggcgaccgc ttaggagaag aaggtaccgg cgcacgcttg
     1441 ctggaagatg aacaaaacta aagcgccaca agggcgcttt agtttgtttt ccggtctttg
     1501 tctttctctc tatcccgctg gtacacagga gggtttcccc cgacgtcaac acacctcatt
     1561 cgagcacgtg gtggaggttc cggttggtgt tgatgcttta attgtatgtc accgacgttt
     1621 cttcgccagt gtaaaagtat actttttaac cgcaatattt ttgtcatctc agacgatttt
     1681 ttatcgcaat cctgaacggt atacggctcg ataacgctgc aatcttgcgc accgacgata
     1741 acgtttgcgc atcaattgcc tgttttttca tcgtcaagac aataaaagag aaaaaagcag
     1801 caaacttcgg ttgaaaaagc cgctatgatc gccggataat cgtttgcttt ttttaccacc
     1861 cgttttgtat gcgcggagct aaacgtttgc ttttttgcga cgcagtaaat tgtcgcaaac
     1921 ctggagcagg aagataacgt ttcgctggca ggggattgtc cgccacgcat cttgacgaaa
     1981 attaaactct caggggatgt tttcttatgt ctacgccatc agcgcgtacc ggcggttcac
     2041 tcgacgcctg gtttaaaatt tcacaacgtg gaagcactgt ccgtcaggaa gtggttgccg
     2101 ggttaacaac gtttctggcg atggtctact cggtcatcgt cgttccaggt atgttgggta
     2161 aagcaggctt cccgcctgcg gcagttttcg ttgcaacctg tctggttgcc ggactcggtt
     2221 ctatcgtgat gggtctgtgg gctaatctgc cgttggcgat tggttgcgcc atctccctga
     2281 cggcgtttac cgcattcagc ctggtgctgg ggcaacatat tagcgtacct gtcgcgctgg
     2341 gtgccgtgtt cctgatgggt gtgctgttta cggtaatttc tgccacgggt atccgtagct
     2401 ggattttgcg caacttgccg cacggtgtgg cgcacggcac ggggatcggt atcggtctgt
     2461 tcctgctgct cattgccgct aatggtgttg gtctggtgat taaaaacccg cttgatggtc
     2521 tgcccgttgc gctgggtgat ttcgcgacct tcccggtgat tatgtcactg gtaggtctgg
     2581 cggtgatcat cggcctggaa aaactgaaag tccctggtgg cattctgctg accattatcg
     2641 gtatctcaat tgtcggtttg atcttcgatc ctaacgtcca tttctccggc gttttcggca
     2701 tgccttcatt gagcgatgaa aacggcaatt cactgattgg cagcctggac attatgggcg
     2761 cgctgaatcc tgtagtcctg ccaagcgttc tggcgctggt gatgacggca gtatttgatg
     2821 ccaccggaac tatccgtgcc gtcgccggcc aggcgaacct gctggataaa gatgggcaga
     2881 tcatcgacgg tgggaaagca ctgaccactg actccatgag cagcgttttc tctggcctgg
     2941 tgggtgctgc tccggcagcg gtatacatcg agtctgcggc gggtacggcg gcgggcggta
     3001 aaaccggttt gacggctatc accgttggcg tgctgttcct gctgattctg ttcctctctc
     3061 cgctctctta cctcgttccg gggtatgcaa cggctccggc gctgatgtac gttggcctgc
     3121 tgatgctgag caacgtggcg aaaatcgact ttgctgattt tgttgatgcg atggcgggtc
     3181 tggttacggc ggtattcatc gtgctgacct gtaacatcgt aacaggcatc atgatcggct
     3241 tcgcgactct ggtgattggt cgtctggttt ccggcgaatg gcgcaagttg aacatcggta
     3301 cggtcgttat cgccgtggcg ctggtgacct tctatgcggg tggctgggct atctaatctt
     3361 tcatcggatt ctgaaaacgg gtggcaatgg ctgcccgttt ttattttctc cgcacattgt
     3421 gtgagctttt gcgatattct gaaaaaatga gaattcaggc ataacgcctt tctcagatca
     3481 taaaaaaaca tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc
     3541 ctcgtggtct cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg
     3601 cttatgcaaa tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag
     3661 tttgatcctg aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg
     3721 aaaacgccga cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg
     3781 ctggtggtgg tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt
     3841 ccgctgatcc ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc
     3901 tccggaattg ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa
     3961 gcgttgatga atgacgcctc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg
     4021 gggacgatga tcttcaccgt cggcggtgcg acggtcgaat ttatgaaagt agccattggt
     4081 ggtattcttg ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc
     4141 cgctggggcg gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct
     4201 tcttatctga ttgccgaaca tattggcgtt tccggcatcc tcgctgcggt tgccgccggg
     4261 atgaccatca cccgctccgg tgtgatgcgc cgtgcgccgc tggcaatgcg cctgcgcgca
     4321 aacagcacct gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt
     4381 ctgcagctgc cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc
     4441 gaaatctgga tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt
     4501 ttcggctggc tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg
     4561 gagtttggtt cgtggaccac acgagaaatc ctgatcgcgt ctttcgccgg ggtgcgtggg
     4621 gcgatcactc tggccggtgt gctctctatc ccgctgctct tgccggatgg taacgtcttc
     4681 ccggcgcgct atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc
     4741 ggtgtggtga tgttgcctat tctgctacaa cacattgaag tcgcggatca ttcgcaacaa
     4801 ttgaaagagg aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag
     4861 aaaatggagg agcgtctggc ggcggatacc gaagagaata tcgataacca gctgcttacg
     4921 gaggtcagtt ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt
     4981 gaaagttccg tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct
     5041 gaacgtgctg aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa
     5101 aaattactgc acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc
     5161 gaaagcgacg taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa
     5221 caggttagct cgggctaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag
     5281 tcaattcatt ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt
     5341 aatcttcata gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt
     5401 acaatcttct tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt
     5461 ttcctctcca cggagtatgg aaatgcccat tccctggacg ctagcctcat caaaattaac
     5521 ccgat
//
LOCUS       DAJVTY010000208         5441 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVTY010000208 DAJVTY010000000
VERSION     DAJVTY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.209"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..915
                     /gene="adeQ"
                     /locus_tag="OQQ66_003779"
                     /gene_synonym="yicO"
     CDS             <1..915
                     /gene="adeQ"
                     /locus_tag="OQQ66_003779"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1906524.1"
                     /translation="IPLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSH
                     GVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIG
                     EVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDS
                     VSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYA
                     TAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMK
                     VCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            968..1420
                     /locus_tag="OQQ66_003780"
     CDS             968..1420
                     /locus_tag="OQQ66_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1906525.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1631..2821
                     /gene="nepI"
                     /locus_tag="OQQ66_003781"
     CDS             1631..2821
                     /gene="nepI"
                     /locus_tag="OQQ66_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1906526.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWVIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2862..3155)
                     /locus_tag="OQQ66_003782"
     CDS             complement(2862..3155)
                     /locus_tag="OQQ66_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906527.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3377..4195
                     /gene="nlpA"
                     /locus_tag="OQQ66_003783"
     CDS             3377..4195
                     /gene="nlpA"
                     /locus_tag="OQQ66_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCS1906528.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4222..5121)
                     /locus_tag="OQQ66_003784"
     CDS             complement(4222..5121)
                     /locus_tag="OQQ66_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1906529.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5232..>5441)
                     /locus_tag="OQQ66_003785"
     CDS             complement(5232..>5441)
                     /locus_tag="OQQ66_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906530.1"
                     /translation="IGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWS
                     YHALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1298 a         1238 c         1395 g         1510 t
ORIGIN      
        1 attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat cgccttaatg
       61 ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat gattggcgat
      121 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca
      181 tcacgtcatt ttcatgccgc ggtgctggtt tctatagtgg tgacgtcttg ctgtggatta
      241 tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc
      301 attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc
      361 tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagcg
      421 ggcttaatag atggtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc
      481 gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg ttactgccta tattgaaagt
      541 acttctggtg tggcagtcgg tggccgcacg gggctgactg cggttgtggt tggcgttatg
      601 ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
      661 ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat
      721 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg
      781 atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt gtgcaccggg
      841 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt
      901 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
      961 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1021 ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agtgatcggc tgaatatcga
     1081 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1141 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1201 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1261 acaaaaagcc cgtcttgccg cgccgattac cgatgctcaa gtcaggctgg cgctgggttt
     1321 tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc
     1381 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1441 ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc cggtaatctt
     1501 tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag ttgcaaaaac
     1561 gttaaatgcg tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac
     1621 gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca tcacccgacc
     1681 gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta tcgttgagtt
     1741 tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc
     1801 cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca
     1861 gacaattcag gctactgacc gctgctacgt tgttattttg tttgccgttt tgctgacgct
     1921 ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg gtcgtgcctg
     1981 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     2041 gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     2101 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     2161 taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg gttatcaaat cattgccttc
     2221 actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     2281 tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac
     2341 gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct taacgctggt
     2401 gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt tcattcttaa
     2461 acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt
     2521 actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta tctgggggct
     2581 aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc tggccgatca
     2641 ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata cctgtggcgc
     2701 ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg
     2761 cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga agaaatcctg
     2821 atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc cgggcattct
     2881 accttaccca acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc
     2941 tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct
     3001 gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt ggttttctct
     3061 tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg catagcgaaa
     3121 aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta caaatgtgta
     3181 tcgaatgaag cataagtgta gtttgctttc atcgctctca tcattctgta cgaaacgcgc
     3241 ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt tttattctta
     3301 tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag gtaatcatta
     3361 ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg ccgcattatt
     3421 gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa aacacattaa
     3481 agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtggcaaa
     3541 agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga
     3601 tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct ttcttgaaca
     3661 agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccctat
     3721 ggcgggttat tccaaaaaaa tcacaaccgt ggcgcaaata aaagaaggcg cgacagtggc
     3781 gatccccaac gacccgacca accttggtcg cgcactttta ctgttgcaaa aagagaagct
     3841 aattactctg aaagagggaa aagggttatt acataccgcg ctggatataa ccgataatcc
     3901 tcgtcatttg cagattatgg aactcgaggg ggcgcagctt ccgcgcgtac tggatgatcc
     3961 caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc tttctccggt
     4021 gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt tggtggcacg
     4081 ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga
     4141 agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct ggtaattctc
     4201 actcatgccg cgccggacga tttatttttc tggccggcga cgggaatcca ttgaaatcaa
     4261 aatcaccgat gacagaatca gcagcgttcc cagccagtcc ggtaatgtga acgtgatccc
     4321 cagcaacagc aaagagagta gcgcgctact taatggttct gcacagctca aaatgctggc
     4381 ttttggaccg ccaattaatt gtgctccttt caggtacaga ctaaatgtca gggacgtacc
     4441 aatgaccacc aaataaaaaa acgccagaat caaactgccg ttaacgacaa agtttgttcc
     4501 ttgtctggca taaaaaggca acagaatcag accgccaatc agcatactcc agccgacgac
     4561 tggtaacgtg ccatagcggg caattagcgt tgagggatag gtggtataga atgcagcagc
     4621 aaatgccgag gcaatgcccc agaacaacgc ggcaggagag agcgataatg acgtcggatt
     4681 accgtgtgtc accaataaaa aagtcccgac cagcgatgtc aaaatagcgc agaaaaccag
     4741 aatgcccggg cgcgatttac gcaccagtga gaaccaggcg acgataatcg tcggtgagag
     4801 gaattgcagc accgttgccg tggctgcgtt cgatttttcg atggttagca aaaaagtgag
     4861 ctgtacagtt agcgcgccaa ccacggaaaa aatcagcagg ctaatggcat ctttatgatt
     4921 gttaataata gaaaagattt tatcgccatg aacaaatgac agcgtcagta gaatcaaacc
     4981 ggcgaatatc aaacgcgtca tagtcaaaaa ctgcgacgac atctggcttt gctccatgat
     5041 gtattgcgcg cagacccctg aacttcccca caacacggcg gcaatcagaa cgttcagcat
     5101 cccctttctg gtggaaccca tttttccctc tgcatactgt tttgtcgttt atgtatgcag
     5161 gcatgatagc aaaaatggcg aggatggctc tgtttgcgcc acgccgattt ttcttagccc
     5221 agactggtga gctaaatatc tttaataaac agcaggcaaa tcatcgcaat acccaacatc
     5281 cctatcgcca gccagaacaa cgcatggtag ctccagattt caaccataat tccgtcaaca
     5341 gagccggcga taatccagcc gacgcgtgaa gtatttgcat ataacgttgt cgcagagcct
     5401 attttttcag gcattaagtc ctgaaaataa agcatgccga t
//
LOCUS       DAJVTY010000209         5441 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVTY010000209 DAJVTY010000000
VERSION     DAJVTY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.210"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..256)
                     /locus_tag="OQQ66_003786"
     CDS             complement(<1..256)
                     /locus_tag="OQQ66_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1906531.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQ"
     gene            815..961
                     /locus_tag="OQQ66_003787"
     CDS             815..961
                     /locus_tag="OQQ66_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906532.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(1121..1438)
                     /locus_tag="OQQ66_003788"
     CDS             complement(1121..1438)
                     /locus_tag="OQQ66_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1906533.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(1439..1696)
                     /locus_tag="OQQ66_003789"
     CDS             complement(1439..1696)
                     /locus_tag="OQQ66_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS1906534.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(2045..2251)
                     /locus_tag="OQQ66_003790"
     CDS             complement(2045..2251)
                     /locus_tag="OQQ66_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906535.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(2248..2709)
                     /locus_tag="OQQ66_003791"
     CDS             complement(2248..2709)
                     /locus_tag="OQQ66_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906536.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(3080..4684)
                     /locus_tag="OQQ66_003792"
     CDS             complement(3080..4684)
                     /locus_tag="OQQ66_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906537.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLENIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(4697..5062)
                     /locus_tag="OQQ66_003793"
     CDS             complement(4697..5062)
                     /locus_tag="OQQ66_003793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906538.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(5090..5299)
                     /locus_tag="OQQ66_003794"
     CDS             complement(5090..5299)
                     /locus_tag="OQQ66_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906539.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         1425 a         1295 c         1258 g         1463 t
ORIGIN      
        1 cctgtttcag aatgcagcgc accgccgcca tctcattttg cagggaacgc ttcgtgatac
       61 cctgcgccag tcgttcacga acatatccct caatataccg cgccttaagc tgctccaccc
      121 ggcggatctg cacattctgt gccagaacca gacgctcaca aaaccgctgc accagcttaa
      181 tacggtcgtg aacggtctta tggctgccgc ccgcctgttg tgccaatcgc ttcatttcct
      241 gctccagaat ccccatacat tccccctttc atgattgccg caaactcatc cccaaaaact
      301 caacactgat acagcgttcc ccggcgcaca ggccgatagc ccctgagttt tgtcggggct
      361 gcgaacgata cctgagcgcc ggggcggcag ccgttgaggt gtggtggggc ttcggttgtg
      421 ctctcagagc agccgcccgc ttgcgcgggt catcccccgg atcgtccaga tccgcctcgg
      481 tatcggttca tattctcctt atgggtaaat ggcctaaaaa ggcggtgtca gaatccagcc
      541 atgagctggc gcagttgagt tttctggtgc aggtcgaaag ggcttgcgtg cgtcactttc
      601 ccgctttgcg cggttaaagt gacgcacgga tctggtgcag catctgagat ttatcgtctg
      661 gtgcagtttt acggtcacgc ggcagcaaag cctgacggca atgcaggccg cagcccgtaa
      721 ggtaaaaggc gctgagggtg tcagcagatg caggttagcc gggagaattc cggcgcggtg
      781 cagattcaga ccagcaggct ggcgaagtaa aaggatgtat cgtattaaaa ctctggcctc
      841 cgtcgcgatg ttctgcgacc cggtgtttgc agaggtctgc accggtaaac ggatcgaccg
      901 tcctgataac aggcgcttct ttcaggttgt gaagcgttgg gcgctgtcta cgacagcata
      961 gagcaagggg gagactacgc ctgtgccgct ggcgcggaaa ggagaaactg tttttacctg
     1021 caaaaggatt ttttaatcac ttgttgttat aaaaaatgag ggcgctgtat gtgacagcgc
     1081 cctcgttcag gttacataaa aaatatcagc tcagaatagc ttacagtcga gccagcacct
     1141 cattcacgac agcatcgggt atacgttcca gccgcttacc gctccgagct cccatatcaa
     1201 tggttctggg ctggtcgcag cgaataacgc ccgttgtttt tgttcccgcc ccgtccagtg
     1261 aaacggtaaa accggcttcg cgggcaaagt ttccgccgct ggtgacaggg acaacaacag
     1321 gcaaccgggt gaacgtgtta aacgatgcct tcgatacgat gagtaccgga cgttttccac
     1381 tttgttcatg gccagtggtg ggatccagtg aaacgagcca gatttctcct ctgtccatct
     1441 acaggatctc cttgcccacc gcaggcgcat caatccattc ccgaccctct tcgcttattt
     1501 cagcgtgcgg atcgcactgc gccaacagtt cttcgagcga atagcgggga cgtttttgag
     1561 gttcaataat caggcagcca ttatcaatgg tcatgcccac ttcgctgtcc gtcgacagcc
     1621 ctagcgtttt cagcactgcg gggggaaccg ccagcatgac ggatccgcca acctttttca
     1681 ggcgagtggt atacatagag cacctccgga tattatattt taatataaca tccatgatag
     1741 acgatgcaca actattcatc agattcattg tggtttcctt agtgtaataa agcgtgaaga
     1801 aagccttaat ccatcggaag acagatccgg cagttttcaa aggcgacatt gccggtaaaa
     1861 gggcaaccgt cctgatacat caggcgcttc tttcaggtca tgaagcattt cgtacaggcg
     1921 ggagcttagt gcgtaacaaa gaaggccgat aataggaaac tgcttttgca gttcaaagga
     1981 ttatgacggt tcggataaac aaaatgcggc gcagcttccg tatccgggct gcgccgcagg
     2041 aacatcagcg gtagcgcgaa ggccagattt gctcaggctt gatgcctaac gcttcggcaa
     2101 ttaacctttc gcctttgggc cagcggcggg taagcgcatt agccagcgtg gaggacgcca
     2161 gacctgcctg acgcgaaagc gctgacagcg aagtgcctcg ttttttcagc ccggcgataa
     2221 tgtcggcagg atgccagtcc tgcagcatca tgcagccacc tcgtcgataa acttcacgcc
     2281 atcggcggtg cggagccagc gcggggcttt catttcggcg gcaaaatacc agaccagttc
     2341 acataataag cctgttatcg tctgctccac ttttggctgc atcccctgcc cgataagaag
     2401 ttgcgccagc gtcagtgagt aatcacaaag taattctgcg tcggggacaa atcggggcat
     2461 atcggcaggt agagcatcaa cggtcaggct gtcagtcaga tgagacggga tggggtcgtt
     2521 cagcgtgggt tgcagcaaag tgagacaggc ggcaagtcgc ccgcaaagcg ccagccgcag
     2581 caccggatct tcgctttcga tcagggtttc ggcgacgttt tcgcagtggt ctgccagtac
     2641 ggtaaaatct gtggcagcac tgaagggaac agcaagtaac gggtgtgtct gagtggggat
     2701 cgtagccata aaggcagcct ccagtatgtg gtttgaaaac caccaccaga ggttccaatc
     2761 tcatgggtgg tggactgaac gaggttggaa ctaccggcca tactggaacc ggcgcacctt
     2821 acggtgcccc cgcccagccc accataattt tggtgtagct aagcgcgcga cataaaaaaa
     2881 gacgcagacg cgtcacgtgt cgccagtatg gttatatttc ggggttccaa tcccggcacc
     2941 tgattttgca ggtgcgggta aaagataccg ctttcggttg aaaacggcaa ggggaagtcg
     3001 tggaatagag atcgcagatt gttataaata tacacaatag ttaatgttaa caaagacggc
     3061 acatatatta actttactct caatctgctg aatagtttat cgcatgtaat tctcgggtca
     3121 tatagtggat accctccttc ttttctttca tcccctcttg cctcaccata aaagcaaacg
     3181 aaaaaattgc tgaacgagtt atctgaagta aatgctcaaa atggtgaata aaatcacttt
     3241 ctttgataaa aagaatatct tctattagtt gataagattc gtaaatgtcc tcaggtttat
     3301 cacttttatg tacaactaaa aacttatgct ctaaaccatt gcgccattct ttatagaaag
     3361 ccaactcccc acccttatct ttattcaggt cggtagcaat actgtacagc gccagaagcc
     3421 ctggtgattt taccttttca aataattgtc ttctgttatc aacatctaac tgccagaaat
     3481 tctgaaaata gatattcttc tgaggtgtac ctttttttgt tgttgggtaa agattataaa
     3541 gttcacatat ggctaccgca attttatcaa gaataccaaa gcaaagacga aatgccgtcc
     3601 tgattttttc aatttctatt cctaaaactt cgtcattata caattccaga aaatggcttt
     3661 cgtacttaat gtaatccgta ttttgtggat gaagataatc aaaataaaga tggcgtgcga
     3721 gtgaaaactc cgatttaagg cgatttaata ccatctccat gggaatgata aaatcacctg
     3781 ttacaccgcc tcgcgaagca atcacaagat catctgtagc actccctaca catgggcaat
     3841 acaggccatg ttctgataaa gtcagatgat ttctaagaca aaactgtcga tatgggctta
     3901 acacttcgaa ctcttgttgt gtaagtgctt catcgtgttc gtcagtttca tcatcaacag
     3961 catattctgc gattttctcg ttagtctgcg ctatgcgttc taaatagaac gattcccatt
     4021 gtggtgggat attttttgat acagacgctt taatatatcc tgctctcact tcttttagta
     4081 acttgattga aaatgaagat gaaataagat tgaggaccat aagtgacatc gaacggttga
     4141 cccaagcctg tggtatatct aatcccttaa gattggtaag atcataatat ctcaatgact
     4201 cactgagccg aaattgactt cttaatgaat tagccaaatt aacagatagt tcagcctgaa
     4261 attcctcctg ctcctcagca gatagcttaa aagaacgcca gtagatattt tctaaggaaa
     4321 ccaattcttc tatcgatttg aatgtatggt cattggggtt agtaatgctc accagattag
     4381 atagcgcatt accatagtta taatagtaaa ggcactctcc taacactgat ataaatgctt
     4441 ctttgttatc ttccatcagg ttgcaaccaa gctctgacgc atttttatta ttttgcatct
     4501 gcccgatgtc aacaagagca cccgcaatgt tcgccaatac tataaagtag ttatcgttcc
     4561 tgattgttat tctttccagt ttgttcagga acgcaactga ctgttgataa gattcgtcaa
     4621 atcttccaat atcaaccagt tggctaatga aattactttc atcgacaagt tcttttgtta
     4681 tcaaaataaa ttttccttat acagaggatt tcggtgcttc aacaggaggt atgccctcac
     4741 aggagacatc tataactatc gctgataagc ggtctctttt atcaaccggc acagtttcat
     4801 taagcatatt ttccattgca ctggcgtttt caggttgaga tgtatttttc gccaaatgag
     4861 gcagggaatt cggtcgagca gaaccaaacc aaaaaaccag atagataccg tgaccagcag
     4921 catcggggtt tttagtataa agcttgtcca attgcccatt aagtgcagtc cagacatcac
     4981 gatgataatc acgctttatc tcgataggta tttttatctg cggtagtgaa acaatgatat
     5041 ctgccctttt atcagaaacc atgtgtcctt ctggttcaca gcttattttt tatggattaa
     5101 gccggacacg gagtaaatcc agaaaaatat tacggcaact ctcttcaggt ttaggcgtta
     5161 gggtacggcc atagctgtct tcattccaga attgcttata aatatcggta ttttcattgg
     5221 caattcggtt agaaatatcc cgtatatgat caagcaataa tgagtaaaga tccattatgt
     5281 tggcaggcgt tttattcatc aatgtactta cagtctgctt ccagtcggca tgatggaact
     5341 gtgattctct ataacgaatt ttctgattgc tttgggcatg tgagagatga tcccgataac
     5401 tttcacattc aggcagcagt aataagcgct caagtacttc a
//
LOCUS       DAJVTY010000210         5090 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVTY010000210 DAJVTY010000000
VERSION     DAJVTY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.211"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OQQ66_003795"
                     /gene_synonym="yiiD"
     CDS             complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OQQ66_003795"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1906540.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OQQ66_003796"
     CDS             complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OQQ66_003796"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1906541.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2311..3184)
                     /locus_tag="OQQ66_003797"
                     /pseudo
     CDS             complement(2311..3184)
                     /locus_tag="OQQ66_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OQQ66_003798"
     CDS             complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OQQ66_003798"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1906542.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3918..4805)
                     /locus_tag="OQQ66_003799"
     CDS             complement(3918..4805)
                     /locus_tag="OQQ66_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1906543.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4795..>5090)
                     /locus_tag="OQQ66_003800"
     CDS             complement(4795..>5090)
                     /locus_tag="OQQ66_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906544.1"
                     /translation="GPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLSPLIA
                     SSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
BASE COUNT         1350 a         1287 c         1205 g         1248 t
ORIGIN      
        1 tctgataaat gaagaaaaac cgtattcatc gccattacca ccaccttacc tatattactc
       61 attaatgtat gtgcgaaaat tacgccggaa aatcattaaa aaaagcggat ttttgcatgt
      121 tgttttgttt ttgcgaggcg ttttccagaa ataataagag tgttgcagtt gtttcattta
      181 ttactcaaaa atggtttagt tatcgcccag gatgaattat gggtgctatg aatatattat
      241 attcagagat atttatttgc ataatctgaa tggtgtagcc gaaaaacgtc agcatcctaa
      301 cagcaccagg atttaaggtg aaattaatct ttcatcacag accggtcagt actgccacca
      361 accggtcttt acttgctcat cgttataatt ttgttggtaa cagttcggga aagttgtttt
      421 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      481 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      541 gtaaatgaat gatactgaca atgatgccag cccattatct ttgcataatg tttgtgagct
      601 atagatttta aaaatattta tgaggaaagt atggcaccag aggaggtttt gactatcaaa
      661 aatcgctcaa acctgaacta agatgatgag ttaagcatta tctgcgataa ttgccactca
      721 gctctacaaa taatatttaa atgctcctga acagtgatgt agcaagacac gtatggtgcc
      781 aacgaagaat aaaaatttat agagttctta ttcaatatta ccggcataga cagggaatga
      841 tgtaaatgac cggaatgaat gagagaggca gcaccgcccc tctccataag ctactcttct
      901 tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg gcagaacgat atacgtgcct
      961 tcaaacactg cacccggcgt ctcgtcgcca aagatttcaa cttgcatctg cacccgtgct
     1021 tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg caccaaggtc ggctaccgca
     1081 tgaggtttac cgctaatcgg cttgctgtag cggatatgcg catcggccag aataatcgtt
     1141 ccgccgaggt ggcgttcgcg cagcatcagc cagataagcc cccaaccggt gagcgttgcc
     1201 agcgagaata aactcccggc aaacagcgta tggtgcggat tctgattacc ggtttccggc
     1261 atggtggtga taaatttttg cccggtatat tgctgaatgc gcacgcccat tttttcacta
     1321 agcgggatgt gttcgtacca cgcctgttgc agctgcgcgc accagtcgcc gcgatgcaaa
     1381 atatcatcca gagtggcaac aggtttaatc atcaaaaaat ggcgaatcgg cgtggtggtt
     1441 ggtgtggtga tttctccctg attaacaaac cccagcttgg cgaaaaactc caccgcgtct
     1501 tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac gcgccaccga ctccagggtc
     1561 atcgccatca acgtccccag ccctttgtcc tgcacgtcgg gatgaacggc cataaagcga
     1621 atggacgctt cattgtcggc attaatatac agtcggccta ccgccaccag attcccctgc
     1681 tcgtcgacga ccatctgatg atgcgccatc gcatcccacg cgtcgcgttc cgaacctttt
     1741 ggttgatgca ggggcttacg caacatttcc cagcgaaact gatagtaacg ctctaactct
     1801 tcttctgttt gtggaacccg aaggtgatac atagcgatac tctctcttgt ttcccgtgac
     1861 aaccctggaa gctggctcat acctgcaacc agaatgtcac ggggccatca ttgaccagcg
     1921 atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt catctcttgc tggcggcagc
     1981 gttcgacgaa atagtcatat aacgcctctg cgcgatccgg tgatgcgcct ttggagaaac
     2041 ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa ctgggaaacc accagtacac
     2101 tgccgcccgc ctgttgcacg ttgagattca tcttgccttc ggcatcgcta aagatgcggt
     2161 agccgagcac acgctcgcac agacggtttg ctttctgttc gtcgtcatcc ttttcgacac
     2221 ccaataacac caaaagtccc gcgccaattt cgcccgtcac ttctccctcc acggtgacgc
     2281 tggcacgggt tacgcgttga attaatgcaa tcatggttcg tcgtcttctt cttgttcagc
     2341 tgcttgtttt agtttgcggt attccccgag agtgacagta atttccgcgc caagcaagac
     2401 gatacaccat gtccagtaga cccaaacaaa gagaatgggg atcaccgcca gcacaccgta
     2461 aatgagctga tatgacggga acatggtgat ataaagcgcg aaacctttct ttcctgcttc
     2521 gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg cggttaggta cgcggatggt
     2581 aggaacaatg ctgtacagca accagaagga gatccacgac aacagcaacg gaaaaatacg
     2641 cagcacgttg tcgatgacag tattgagatc gctcgcccag cgcagggaga gcaaatagga
     2701 actgatcgcc agactggccc ctgccagcag cggccccagc gttaaaatca tccagtacac
     2761 ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc cagatggtat tcaacgcgct
     2821 atcgatggag tacatcaaca ataacgcggt gacgatcagc ccgcacgccc caacggcggt
     2881 catcttgttg gaattggcaa caaattgttc gatataccgc tgaataacat cgccagtagc
     2941 aggcagaaag ttggcaaaaa taaagtgacg taactgaatg ctgacgtcgg aaaacatggg
     3001 aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc actaatgaga gcaacgacca
     3061 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     3121 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     3181 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     3241 aatgcccagc tgattggctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3301 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3361 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3421 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3481 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3541 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3601 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3661 atgaaaactc ttcttaagcg tagccagtgg aacacgcgtt aaatcgctcc aggctcccag
     3721 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3781 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3841 gcaataaggg taatcttgtg atgctgattg cgaaaaagca gagtaatttc actggctttt
     3901 tcgcaataga tgaatggtta agattcaggt gtaaattgcc aacgagtttc gatgaccagc
     3961 ggcctatctg gtgtcaccaa ttccggcgtt agatttattg cgttaggtgc ctgagtcagc
     4021 gggttaatac aggtggcatc gggctgttta tcaaatacca ccagactgtt tgcagaagag
     4081 atcatggtca tcgcgagttt accaggccac agtaacttaa ccttcacccc gttataaaaa
     4141 ccaaaacagt catcccaggg accggcttga ggagagatac gatttccagt tggcagttca
     4201 tcactacccg cttcttcttg ccagtccgca tcaaaaagca cctgcaacga ttctgtattt
     4261 tgcggagtca gctttttcgc aaaccacgga tgccagccag cggaggccgg gaatgtatcg
     4321 gcatatgaat ggacttccag ttgcaaaacc agcgcatcgt tctccagcaa aaaagtctga
     4381 atcacttccc cttgccaggg ccacggtgaa gccaggggca tacgtaaagt cagggaatct
     4441 gctgttttgt cgatgatttc ccacgttgag taacaagcca taccatgcaa cgcatgtggt
     4501 ggtttattgg cgggtaaaga ataacattgc ccaccagcat tcagcgtcgc attccccagt
     4561 cgccctgccc acggcaccat tgggaaacag ccatactgaa aagctctgcg ctgcggctgc
     4621 cattggcgta atacttcaga accaaatgcc tttaacgagg ttatcctggc accatcatgc
     4681 ggataaatct gcaggtgaat attgcctttt tccagcgtca ctaactctcc tacaggccca
     4741 ggccatggcg cgtcaaacgc ctgttgtagc gccaggtaat gctcgttttt tcgttcagtt
     4801 ttcatgagat tgttccctga tgtagcggta agaagcggtt tcttgttcat cggtcggctc
     4861 aatcagccag acgcaaacaa aggaaagaac tgagacagca aataaaaaca gagcgacata
     4921 ccacggtttt cctcccccta gcgccagcaa acttgaggcg ataagggggc ttagtccgcc
     4981 aagaatcgcg gaaaattgat aagcaaaaga aaggccggta tatcgtactt tggtaccgaa
     5041 catgcgggtg aaaagtgttg gctgcacggc aaacatcatc gtcggaccta
//
LOCUS       DAJVTY010000211         4896 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVTY010000211 DAJVTY010000000
VERSION     DAJVTY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.212"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..237)
                     /locus_tag="OQQ66_003801"
     CDS             complement(<1..237)
                     /locus_tag="OQQ66_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCS1906545.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARF"
     gene            495..1274
                     /gene="deoC"
                     /locus_tag="OQQ66_003802"
     CDS             495..1274
                     /gene="deoC"
                     /locus_tag="OQQ66_003802"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1906546.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1401..2723
                     /gene="deoA"
                     /locus_tag="OQQ66_003803"
     CDS             1401..2723
                     /gene="deoA"
                     /locus_tag="OQQ66_003803"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1906547.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2775..3998
                     /gene="deoB"
                     /locus_tag="OQQ66_003804"
     CDS             2775..3998
                     /gene="deoB"
                     /locus_tag="OQQ66_003804"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1906548.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4078..4797
                     /gene="deoD"
                     /locus_tag="OQQ66_003805"
     CDS             4078..4797
                     /gene="deoD"
                     /locus_tag="OQQ66_003805"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1906549.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1173 a         1251 c         1355 g         1117 t
ORIGIN      
        1 aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac cttcaaacat
       61 atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct gcggataagg
      121 cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc taagcactgg
      181 gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag tgggcatctt
      241 cttttccttt tatgccgaag atgatgcgcc attgtaagaa gtttcgtgat gttcactttg
      301 atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt tgaaccagat
      361 cgcattacag tgatgcaaac ttgtaagtag attaccttaa ttgtgatgta tatcgaagtg
      421 tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta ttggcgacaa
      481 gccaggagaa tgaaatgact gatctgaaag caagcagcct gcgtgcactg aaattgatgg
      541 acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg tgtcatcaga
      601 ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt atcccgattg
      661 ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg gtaaccaact
      721 tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg gcaatcgcct
      781 acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg ggtaacgagc
      841 aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg aatgtactgc
      901 tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt aaagcgtctg
      961 aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa gtggcagtga
     1021 acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg ggcgtagaaa
     1081 aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc
     1141 tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac taccgctttg
     1201 gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac ggtaagagcg
     1261 ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca ggcctacgag
     1321 acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc gcctcatact
     1381 cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa aaacgtgatg
     1441 gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc gacaacacta
     1501 tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat atgacaatgc
     1561 ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc gactggaaaa
     1621 gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc ggcgatgtca
     1681 cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg atgatctccg
     1741 gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc cctggcttcg
     1801 acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc gtggcgatta
     1861 tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc cgtgatatta
     1921 ccgcaaccgt ggactccatc ccgctgatca ctgcctcgat cctggcgaag aaactggcgg
     1981 aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt atgccgacct
     2041 acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc gctggcgtgc
     2101 gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca ggtaacgcgg
     2161 ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg cgtctgtttg
     2221 atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg aaagatgacg
     2281 ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca gaagtctttg
     2341 gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac gcgaagtatc
     2401 tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt gtcagtgaaa
     2461 tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt cgtcaggcat
     2521 ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc gaccaagtag
     2581 acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg caggaagcgg
     2641 cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc acaccaactg
     2701 tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt tgaagcactg
     2761 agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc ggcatcggcg
     2821 ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt catatcgcag
     2881 aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat ctgccaaatc
     2941 tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt ccggcgggaa
     3001 tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg tcatccggta
     3061 aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt gagtggggat
     3121 acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg gtcgaacgcg
     3181 ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt ctggatcagc
     3241 tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct gactccgtgt
     3301 tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa ctgtgcgaaa
     3361 tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc gctcgtccgt
     3421 ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac ttggctgttg
     3481 agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt caggtggttt
     3541 ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa gtgaaagcaa
     3601 ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg ggtgataaca
     3661 ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt cgcgacgtcg
     3721 ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg atgtctttgc
     3781 tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg acctggaccg
     3841 gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa gtaaaaccgg
     3901 gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca aaatattttg
     3961 gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg cttgatgccc
     4021 tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga taaaacaatg
     4081 gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt gatgccaggc
     4141 gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg tgaagtgaac
     4201 aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat ttccgtaatg
     4261 ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat caccgatttc
     4321 ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca cgtaaaactg
     4381 cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg catccgtttt
     4441 aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc ggtagacgcg
     4501 gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga cctgttctac
     4561 tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg cgtggaaatg
     4621 gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct gaccatctgt
     4681 accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg tcagaccacc
     4741 ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa agagtaattg
     4801 tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc cggcttttta
     4861 gtatctattc atcaatagca actgatttat agattt
//
LOCUS       DAJVTY010000212         4814 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVTY010000212 DAJVTY010000000
VERSION     DAJVTY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.213"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..4040)
                     /locus_tag="OQQ66_003806"
     CDS             complement(<1..4040)
                     /locus_tag="OQQ66_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1906550.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASRDAINGSQLYTTNKYIADALDGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVA"
     gene            complement(4084..4767)
                     /locus_tag="OQQ66_003807"
     CDS             complement(4084..4767)
                     /locus_tag="OQQ66_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1906551.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT         1096 a         1190 c         1132 g         1396 t
ORIGIN      
        1 gctacgcgct catcagtgac tttcaactga cccacgttaa ccgcatcggt atccgctgaa
       61 ccagctgcga cgtttgtgat ctgacgttga ccatccgcac taccgactga gaattcaccg
      121 acagagttct gcggtgcgtc catgttgtag gcggtataat tggtttgagc gccacgagtg
      181 gtcgtagaac cattacctat cgcaatactg ttgacatgaa tgacttgcgc attgctacca
      241 atggcaatgc cgttaagtgc attcgcgtcc acgtaggcac cataaccgat accaatacct
      301 ttatcgccat tcacgatggt tttcgcaccc attgccagcg agtcagttcc cactgccagt
      361 gaatcttctt ccgttgaatt agcgtggaag tatttagtcg gcgtggttgc gaccgcacca
      421 atcgcattct gcaattgacg aaccgtaacg gcgtcatggg cttcggaacc atcggctacg
      481 ttgatgattt gacgatattt accgtcatca ccgatagaca atgcaccgag cagttcgcca
      541 tccgtggtgt cgtaaccaat aacaacgcca ttttccgtta tgctggtgtc acgggagcct
      601 ttggcaatca ctcggctgga aacagagctg ctacctaggg cgatacccgt atcaacgtcg
      661 ctgtagctgc cctgaccaat agctacgctg ctatcgcctt tggcgacaga gttataacct
      721 atagctgtag cgccaacacc ctgtgcgctg gcgtcgttga acgctaagcc gtcgtcgtta
      781 gtacgcacat agttaatacc cgcaccgttt tcttggatat aggttgcgtc ggtgttacca
      841 gcgagctgat tgataatttg ggtgttctgc tcaatcatca tattcgtcgc attcaactga
      901 gaaccgttca ctgcatcggt actgtcctca ctaatactgc cattagcgac attagtgatg
      961 atgctggctt taccgtcatg gctggcattg taggctccag caccgccatt ggcagtctca
     1021 tcccacagca gggcgttatc atcaagcgca tccagagcgt caccgacgtt gttgtactcg
     1081 gcgttcgcaa tggtgtaagt cggtgcggtg atggtgccgt cagcgttgac ttctgcgtca
     1141 ccatccagcg catcagcgat gtacttattg gtggtataga gttgtgagcc gttaatcgcg
     1201 tctctgctgg cagcagagat tgcaccgtta gcgacgttag tgattacact ggcagtttta
     1261 tcttttccgt gagcggcgct aaatgcacca ttttcaccgg cgtccgcatc ccagagcaga
     1321 gcgtcgtcaa gagtggtatc gatagcattc agggcatcac cgacattatc gtaatcagca
     1381 ttagcaatgg tgtacgtcgg cgcagtgatg gtgccgtctg cattgacttc ggcaccaccc
     1441 cccagcgcat caacaacata actgctcacg ccgtggagtt gtgagccgtt tactgcgtca
     1501 gaactggagt ctgaaatttc accatctgcg acatcagtga tcacgcttgc gtcaccatta
     1561 gtaccgtgtt tggcactgaa tttacctgcg gtggcatccc aaagcagagc atcgccgaga
     1621 gacgtgctaa atgaggagtt aatcgcagcc agtgcatcgc cgacgttata ataatttgtt
     1681 tcaccgattt tgtaggttgg accggtaaac acaccgtcag tattcacaga agcattgccg
     1741 ccgaagtagg tggcgatatt gctgcttaag tcataaagct ggctaccgtt aatggcatcg
     1801 gaactggttg cagtgactgt gccgtccagg atattggtga ttttgctggc ggtattgttg
     1861 ccatgagctg cagtgaagac accattgtcc ttatcccatt tcagcgcatc ctcaccaaga
     1921 ttagtcaccg tctcagtcag attagagatg ttggtggtgt tagtggcgat attggtggta
     1981 ttcgtcgcca cggcatcgtt ggtggttttc agctgagagc cgttaacggc atcggtgctg
     2041 ccagtcgtca ggtcgccatc tttaacgttg gtgattttgc tggtggtctc ggtgccatga
     2101 gctgccgtga atacaccatt gtccttatcc catttcagcg catcctcacc aagattagtc
     2161 accgtctcag tcagattaga gatgttggtg gtgttagtgg caatattgga agtgttattg
     2221 gcgatattgg tggtattcgt cgccacagca tcgttggtgg ttttcagctg agagccgtta
     2281 acagcatcag tgctgtcagc cgtcaggtcg gcatctttaa cgttggtgat tttgctggtg
     2341 gtatcctggc cgtgagctgc actgaatgcc tttttagttt cgttccagag cagggcatca
     2401 gcctgaaggt caccaacgga atccgtcagg ttggtgatat tggtggtatt ggtggcaata
     2461 tttgccgtat tcgtggcaat gttgctggta ttagtagcga tattggcggt gttggcttcg
     2521 acatcgtcat tggtagcttt cagttgggaa ccgttaaccg catccttact ggaggctgaa
     2581 atcgtgccat ccgcaacatc ggtgattacg ctggcagttg ggctactagt accatgagca
     2641 gcgctgtatt tgcctttggt ttggtcccat tgcagggtgt tttcatcaag tacagcgagc
     2701 gcagccccta cgttattttt gctaccattt tttaaattgt aggttggtgc tgtaacagta
     2761 ccgtcatcaa catctactgc agcccctcct ccaagccttt ttgctaccga gtcgctaatg
     2821 gcataaagct gtgaaccatt aatggcatcg taactgtcag acttgatcgc gccatttttc
     2881 acattaacga tcttgcgttt taatgaacta ttgccgacag agacggtatt gtctaaatcc
     2941 gcaatggaat ctgcgcccag cgcgacagag ttaataccgt tggcactgct attactacca
     3001 agcgcaagac tcttatcacc agttgcatta ctggctgtgc ctaaggcaat ggtgtttaag
     3061 cctctcgact ggctaccatt acccagagcg atggcatcca cgccatcagc tttgttgccc
     3121 tggcctatgg cgattgcatt atctgccaga gcctggctgc catttcctaa tgcgatagta
     3181 ttgactttat cggcaaggct tccgaacccc attgccatgc tattagtccc ttttgccttc
     3241 gcattattgc caatagcagt tgcattttca gcggcttcgg tctcagcacc tatcgcaata
     3301 gcgttcgctg cgctggcaag tgattgtctg ccaaatgcga ggctgtcagt accgctagct
     3361 acactgctac gccccagggc aattgcgttt tcttcagatg cgacgctact tgctcccagc
     3421 gccattgaat acgcttttga cgcattggcg gtgtcgccaa gagcaatact cataatctca
     3481 gtagctttag tggcatttcc cagggcaatg gcgtttgctc cttccgcttt ggaagagtcc
     3541 cccatagcaa tagacaattt acccaatgct tttgaataac ggccgagggc cattgagtat
     3601 tggcccaatg aatacgaaga ggcacccagt gctatacttc tatcgccttc tgatattgca
     3661 ctaaccccaa aggccattga tgcaccacca atcgcatggg tttttgaccc gatggcagag
     3721 ctatattggc cttcagctat agcgtcataa cccacagcag tactggaacc actggtgttc
     3781 ataaaagtat ttgctttcgc ccctttgcct atagcaaccc agccgtcgcc agctgatcca
     3841 ctaccgtaat caacaccctg accgttgtca ttcccggcat tcgccaatgc cccaaacgac
     3901 gacaacattc cacccgcaac cagcgcagaa attaacagct tactgcgccc agatttcttg
     3961 ccacggcttt ttgccgtttc gctggtaaca gtataattcc ctgtcgcagg gttccagata
     4021 actttaaata ttttgttcat taatgatgct cgctattcct tgttataagt gtactaactg
     4081 catttatttt ttgcttcttt tctgccgatt ttcagcagag ggaagattgt cgaactgaat
     4141 attttccatg ttgatggcta acttcagata tcccaggtta ttgggcgaga cacccttcag
     4201 ggtcaaatta atgtcttgcc attgacctgg taataatttc aatgtggatt tcattgatgt
     4261 gttgaaaagt aattgttgcc accgggcaaa ctctttggca tcgacaggtt tttcaccacc
     4321 ccatgtcgca ttaaaaccaa tatttgagat aggtgtactt aataagttga ccagtgaaat
     4381 atgaataaca ctgccattct ctgtggcgtc aatggataac actcgacccg ctaaagccac
     4441 cgaaccattg ttcaggatga aatacttcgt ttttgctggg ttaaactcgg taaaaataaa
     4501 agtgttttgt tgatctttaa cggatttaat tacgtgagtt aaagtctttg tactgcttat
     4561 tatgccagtt tgttgttttt gaatagtttc cagttgactc tttagctgtg cttgttgttt
     4621 ttcaagtaga tcaattttat tgttatgttc ttcttgttgt ttcaataaac tactttctgg
     4681 cttgccttct tcaatgtagt cacatcccgt taatgcgcag agaactgaaa cagtgacagc
     4741 actgaaaaaa atcttcttca agttcatgta aaaatcctta tttcctccga ccagcgatca
     4801 atgccggtag agtt
//
LOCUS       DAJVTY010000213         4783 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVTY010000213 DAJVTY010000000
VERSION     DAJVTY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.214"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..790
                     /locus_tag="OQQ66_003808"
     CDS             <1..790
                     /locus_tag="OQQ66_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HCS1906552.1"
                     /translation="NLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQ
                     GKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVN
                     VSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLK
                     QSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGT
                     YNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1158..2813
                     /gene="lldP"
                     /locus_tag="OQQ66_003809"
     CDS             1158..2813
                     /gene="lldP"
                     /locus_tag="OQQ66_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1906553.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2813..3589
                     /gene="lldR"
                     /locus_tag="OQQ66_003810"
     CDS             2813..3589
                     /gene="lldR"
                     /locus_tag="OQQ66_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1906554.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3586..4776
                     /gene="lldD"
                     /locus_tag="OQQ66_003811"
     CDS             3586..4776
                     /gene="lldD"
                     /locus_tag="OQQ66_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1906555.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1051 a         1270 c         1298 g         1164 t
ORIGIN      
        1 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
       61 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
      121 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
      181 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
      241 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg ccaccgatgc
      301 ggttaacgtg tcgcagctga aatcttctga agcaggcggc gtgcgctacg acaccaaagc
      361 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
      421 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
      481 gcaaagcgtg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
      541 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
      601 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
      661 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
      721 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
      781 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
      841 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
      901 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
      961 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     1021 tacacaacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
     1081 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     1141 actccaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     1201 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     1261 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     1321 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     1381 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     1441 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     1501 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     1561 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     1621 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     1681 ttccaatcct ggttgccgga caagtaacag gcatcgacag ttttgagatt ggccagatgg
     1741 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     1801 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcgggc ggctcgtttg
     1861 ccatcgctca gtaccttagc tccaacttca ttgggccgga gctgccggac atcatctctt
     1921 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     1981 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     2041 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     2101 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     2161 tgatcaacat tccggtgccg tacctcgata aactggttgc ccgtatgcca ccagtggtca
     2221 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     2281 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     2341 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     2401 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     2461 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     2521 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     2581 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     2641 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     2701 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     2761 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     2821 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     2881 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     2941 atcacgtaat tcactacgcg aggcgctggc aaaactagtg agcgaaggcg tgctgctcag
     3001 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     3061 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     3121 agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg
     3181 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     3241 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     3301 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     3361 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     3421 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     3481 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     3541 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     3601 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     3661 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     3721 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     3781 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     3841 cgtcgtggcg aagttcaggc agccaaagcg gcggacacgc atggcattcc gtttactctc
     3901 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     3961 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     4021 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     4081 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     4141 gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt
     4201 aatatctcag cttatctcgg caaaccaacc ggactggaag attacatcgg ctggctgggg
     4261 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     4321 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     4381 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     4441 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     4501 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     4561 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     4621 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     4681 tcgatcagtg aaattacgca agattcgctg gtgcaagggc tgggtaaaga gttgcctgcg
     4741 gcactggctc ccatggcgaa agggaatgcg gcatagtcgt ttg
//
LOCUS       DAJVTY010000214         4736 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVTY010000214 DAJVTY010000000
VERSION     DAJVTY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.215"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            80..1042
                     /gene="tauA"
                     /locus_tag="OQQ66_003812"
     CDS             80..1042
                     /gene="tauA"
                     /locus_tag="OQQ66_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1906556.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1055..1822
                     /gene="tauB"
                     /locus_tag="OQQ66_003813"
     CDS             1055..1822
                     /gene="tauB"
                     /locus_tag="OQQ66_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1906557.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1819..2628
                     /gene="tauC"
                     /locus_tag="OQQ66_003814"
     CDS             1819..2628
                     /gene="tauC"
                     /locus_tag="OQQ66_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1906558.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            2643..3494
                     /gene="tauD"
                     /locus_tag="OQQ66_003815"
     CDS             2643..3494
                     /gene="tauD"
                     /locus_tag="OQQ66_003815"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1906559.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNCVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(3600..4574)
                     /gene="hemB"
                     /locus_tag="OQQ66_003816"
     CDS             complement(3600..4574)
                     /gene="hemB"
                     /locus_tag="OQQ66_003816"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1906560.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1058 a         1219 c         1385 g         1074 t
ORIGIN      
        1 gcgataacga ctaattcttt taatgaatgt ttttattcct gaatactgct cccataacaa
       61 gactggggag cagacaatca tggcaatttc atcgcgtaac acacttcttg ccgcactggc
      121 attcatcgct tttcaggcac aggcggtgaa cgtcaccgtg gcgtatcaaa cctcagccga
      181 accggcgaaa gtggctcagg ccggcaacac ctttgctaaa gagagcgggg cgagcgtgga
      241 ctggcgtaag tttgacagcg gagccagcat cgtgcgggcg ctggcttctg gcgacgtgca
      301 aatcggcaac ctcggttcca gcccattagc ggttgcagcc agccaacagg tgccgattga
      361 agtcttcttg ctggcgtcaa aactgggtaa ctccgaagcg ctggtggtaa agaaaactat
      421 cagcaaaccc gaagatttga ttggcaaacg cattgccgta ccgtttatct ccaccaccca
      481 ctacagcctg ctggcggcgc tgaaacactg ggggattaaa ctagggcaag tggagattgt
      541 gaacctgcag ccgcccgcga ttatcgccgc atggcagcgg ggtgatattg atggtgctta
      601 tgtctgggca ccggcggtta acgccctgga aaaagacggc aaggtgttga ccgattctga
      661 acaggtcggg cagtggggtg cgccaacgct ggacgtctgg gtggtgcgca aagattttgc
      721 cgagaaacat cctgaggtcg tgaaagcgtt cgctaaaagc gccatcgatg ctcagcaacc
      781 gtacattgct aacccagacg cgtggctgaa acagccggaa aacatcagca aactggcgcg
      841 tttaagcggc gtgccggaag gtgacgttcc ggggctggtt aaggggaata cctatctgac
      901 gccgcagcaa caaacggcag aactgaccgg accggtgaac aaagtaatca tcgacaccgc
      961 gcagtttttg aaagagcagg gcaaggtccc ggctgtagcg aatgattaca gccagtacgt
     1021 tacctcgcgc ttcgtgcaat aaaaggaggc gcagatgctg caaatctctc atctctatgc
     1081 cgattatggc ggcaaaccgg cactggaaga tatcaacctg acgctggaaa gcggcgagct
     1141 actggtggtg ctggggccgt ccggctgcgg taaaaccacg ttgctgaatc tgattgccgg
     1201 ttttgtgcct tatcagcatg gcagcattca actggcgggt aagcgtattg agggaccggg
     1261 agcagagcgc ggggtggttt ttcagaatga agggctgcta ccgtggcgca atgtacagga
     1321 caacgtggcg ttcggcctgc aattggcagg tatagagaaa atgcagcgac tggaaatcgc
     1381 gcaccaggtg ctgaaaaaag tggggctgga aggcgcagaa aaacgctata tctggcagct
     1441 ttccggtggt caacgtcagc gggtggggat tgcccgcgcg ctggcggcga atccccagct
     1501 gttattactc gacgaaccgt ttggtgcgct ggacgccttc acccgcgacc agatgcaaac
     1561 tctgctgctg aaactctggc aggagacggg caagcaggtg ctgttgatta cccacgatat
     1621 agaagaagcg gtgtttatgg cgactgaact ggttctgctt tcatccggcc ctggccgtgt
     1681 gctggagcgg ctgccgctca actttgctcg ccgctttgtt gcgggagagt cgagccgcag
     1741 catcaagtcc gatccacaat tcatcgccat gcgcgaatat gttttaagcc gcgtatttga
     1801 gcaacgggag gcgttctcat gagtgtgctc attaatgaaa aactgcattc gcggcggctg
     1861 aaatggcgct ggccgctctc gcgtcaggtg accttaagca ttggcacgtt agcggtttta
     1921 ctcactgtat ggtggacggt ggcggcgctg caactgatta gcccgctgtt tttgccgccg
     1981 ccgcaacaag tactggcaaa actgctcacc attgccggac cgcagggatt tatggacgcc
     2041 acgctgtggc agcatctggc agccagcctg acgcgcattg tgctggcgct actcgcggcg
     2101 gtggtgatcg gtattccggt cgggatcgcc atggggctaa gcccgacggt gcgcggcatt
     2161 ctggatccgg taatcgaact ttatcgtccg gtgtcgccgc tggcttattt gccgctgatg
     2221 gtgatctggt ttggtattgg tgaaacctcg aagatcttac tgatctattt agcgattttt
     2281 gcaccggtgg cgatgtcggc gctggcgggg gtgaaaagcg cgcagcaggt tcgcattcgt
     2341 gccgcccagt cgctgggtgc cagccgtgcg caggtgctgt ggtttgtcat tttgcccggc
     2401 gcgctgccgg aaatcctcac cggattacgc attggtctgg gtgtgggctg gtctacgctg
     2461 gtagcggcgg agctgattgc cgcgacgcgc ggtttaggat ttatggttca gtcagcgggt
     2521 gaatttctcg cgactgacgt ggtgctggcg ggaatcgcgg tgattgcgat tatcgccttt
     2581 cttttagaac tgggtttgcg cgcgttacag cgccgtctga cgccctagca tggagaagta
     2641 caatgagtga acgtctgagc attaccccgc tggggccgta tatcggcgca caaatttcgg
     2701 gtgccgacct gacgcgcccg ttaagcgata atcagtttga acagctttac catgcggtgc
     2761 tgcgccatca ggtggtgttt ctgcgcgatc aagccattac gccgcagcag caacgcgcgc
     2821 tggcccagcg ttttggcgaa ctgcatattc accctgttta ccctcatgcc gaaggggttg
     2881 acgagatcat cgtgctggat acccataacg ataatccgcc agataacgac aactggcata
     2941 ccgatgtgac atttattgaa acgccacccg caggggcgat tctggcagct aaagaattac
     3001 cttcgaccgg cggtgatacg ctctggacca gcggtattac ggcctatgag gcgctctctg
     3061 ttcccttccg ccagctgctg agcgggctgc gtgcggagca tgatttccgt aaatcgttcc
     3121 cggaatacaa ataccgcaaa accgaggagg aacatcaacg ctggcgcgag gcggtcgcga
     3181 aaaatcctcc gttgctacat ccggtggtgc gaacgcatcc ggtgagcggt aaacaggcgc
     3241 tgtttgtgaa tgaaggcttt actacgcgaa ttgtcgatgt cagcgagaaa gagagcgaag
     3301 ccttgttagg ctttttattc gcccatatca ctaaaccgga gtttcaggtg cgttggcgct
     3361 ggcaaccgaa tgatattgcg atttgggata actgcgtgac tcagcactat gccaatgccg
     3421 attacctgcc acagcggcgg attatgcatc gggtgacgat tcttggggat aaaccgtttt
     3481 atcgggcggg gtgatacgag tgcggacggt tccctcgccc ccttggggag agggttaggg
     3541 tgagggggcg ttcaccgtac tttcaacagg ttaactcccc ctttctgaga ggaaaaaaat
     3601 taacgcagaa tcttcttctc agccaaatcc agcgcaaagt agctgaaaat cagatccgca
     3661 cccgcacgct taattgaacc taagctttcg agcacgactt tctcttcatc tatagcaccc
     3721 gccagcgcgg cgaacttaat catcgcgtac tcaccgctca cctgatacgc gccaatcggc
     3781 aattcagtac gttcacgcag ctcacgcacg atgtcgaggt acgctccagc aggtttaacc
     3841 atcaggcagt ctgcgccctg ggcttcatcc agcagtgact cacgaatcgc ctcgcgacgg
     3901 ttcatagggt tcatctgata gcttttgcgg tcgcctttta atgcgcttcc ggcagcttca
     3961 cggaacgggc cataaaagga agaggcgaac ttggtcgaat acgacataat cgccgtatct
     4021 ttaaagcctg cagcgtccag cgcctgacga atcgcctgta cctggccgtc catcgcggcg
     4081 gaaggggcga tgaagtctgc gcctgcagca gctgcaacca cggcttgctt gcctaaattt
     4141 tccagagtcg cgtcgttgtc gacgccatgc tcgcacagca caccgcagtg accgtgagaa
     4201 gtgtattcac agaaacaagt gtctgacatg acgatcattt ctggcacagt ctgcttgcag
     4261 atgcgcgaca tacgcgccac cagtccatct tcccgccagg cgtcgctgcc ggtttcatcg
     4321 gtatggtgag agatgccgaa agtcatcacg gaacgaatac cggcgttggc gatgcgttca
     4381 atttcgcgtg ccagatgttt ctctggaatg cgcatcacgc ctggcatggc ttcaacggct
     4441 ttgtagtcgt caatttcttc ttcaacaaag atcggcaaca ccaggtcgtt aaggctaagt
     4501 gttgtctctt caaacatagc gcgcagcgca ggagatttgc gcaggcgacg agggcgttgg
     4561 attaagtctg tcatggtctg cctgatgttt gtggaattaa ggtgcacagt atacctgaag
     4621 cggggcagga gtgttttacg aaagttgtcg tgatgttgac aagaagagaa tgaaagagag
     4681 accaggacat ctttgtaata gaaatagcaa taagttcata ttcactgaaa atattt
//
LOCUS       DAJVTY010000215         4695 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVTY010000215 DAJVTY010000000
VERSION     DAJVTY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.216"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            740..1809
                     /locus_tag="OQQ66_003817"
                     /pseudo
     CDS             740..1809
                     /locus_tag="OQQ66_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            1870..4293
                     /locus_tag="OQQ66_003818"
     CDS             1870..4293
                     /locus_tag="OQQ66_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCS1906561.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKCGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNTHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMLDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4304..>4695
                     /locus_tag="OQQ66_003819"
     CDS             4304..>4695
                     /locus_tag="OQQ66_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1906562.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELGCKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYS"
BASE COUNT         1277 a         1174 c         1186 g         1058 t
ORIGIN      
        1 ctgtgccatg ccctacacct acggcagtaa tgatggcaac agcaccacgg atattgaaaa
       61 cagcaagctg gtcgtgatgt ttggtaacaa cccggcagaa acccgcatga gcggcggtgg
      121 catcacttat cttcttgaaa aagcgcgcga gaaatcgaac gccaaaatga ttgttatcga
      181 tccgcgatat accgatacgg ctgctggtcg tgaagatgaa tggcttccta ttcgcccggg
      241 caccgatgcc gcgctggttg cgggtattgc ctgggtattg attaacgaaa atctcgttga
      301 tcaacctttt ctcgataaat actgcgtcgg ctatgacgaa aaaaccttac ccgcagatgc
      361 accaaaaaat ggtcactata aagcctatat tcttggtgaa ggtgatgata atacagcgaa
      421 aacgccgcag tgggcttcgc aaattaccgg tatcccggtg gaccgtatca tcaaactggc
      481 gcgtgaaatt ggcacagcaa aacccgctta tatctgccag ggctgggggc cgcaacgcca
      541 ggcaaacggc gaactgactg cacgcgctat tgctatgttg cctattttga cgggcaatgt
      601 cggcatcagc ggcggaaata gtggcgcgcg tgaatcgacc tataccatta ccatagaacg
      661 cctgccggtg ctggataatc cggtcaaaac gtcaatctcc tgcttcagct ggacagatgc
      721 tatcgatcat ggtccgcaaa tgacggcaat ccgcgacggc gtccgcggca aagataaact
      781 ggatgtgccc attaagttca tctggaacta cgcgggaaat accctcgtta atcagcattc
      841 tgacatcaac aaaacgcatg aaattctgca ggacgaatcg aaatgcgaaa tgattgtggt
      901 catcgaaaac tttatgacct catcggcaaa ctgccagacc tgatgaccgt tgagcaggaa
      961 gatattattc ctaacgacta cgccggtaac atgggatatc tcattttcct ccagcctgtc
     1021 accagcgaaa aattcgaacg caaaccgatt tactggatcc tgagtgaagt cgcgaaacgt
     1081 cttggacctg acgtctatca aaagttcaca gaaggtcgca cgcaggaaca atggttacaa
     1141 catctgtacg ccaaaatgct tgccaaagat ccggcgttac cgtcttacga cgaactgaaa
     1201 aaaatgggta tctataagcg taaagatccc aatggccatt ttgtcgccta caaagcattt
     1261 cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg gtaagattga aatttattcc
     1321 agcaggctgg cggaaattgc ccgtacctgg gaactggaaa aagatgaagt gataagccca
     1381 ttgccagttt atgcctcaac ctttgaaggc tggaactccc ctgaacgtag aaccttccca
     1441 ctgcaactgt ttggtttcca ttacaaatcc cgtactcact cgacctacgg caatattgat
     1501 ctcctgaagg ctgcctgccg tcaggaggtg tggatcaacc ctatagatgc gcagaaacgt
     1561 gggattgcca acggcgatat ggtgcgggtg tttaaccatc gtggcgaagt tcggctacca
     1621 gccaaagtaa caccacgtat tctccctgga gttagcgcta tgggccaggg agcctggcac
     1681 gaggccaata tgtctggcga taaaatcgac catggcggct gtgtgaatac gctaaccact
     1741 ctgcgccctt caccactggc gaagggaaac ccgcagcaca ctaatctggt cgagatcgaa
     1801 aaaatataac ccacgacaac cataaattct ggcatgacat tttgtttgaa aagcaataag
     1861 tgagtaatga tgaaaatcca taccacagag gcgctcatga aggctgaaat cagccgtaga
     1921 agtctgatga aaacctccgc acttggcagt cttgcgctgg caagcagtgc tttcactctg
     1981 ccattttccc aaatggtccg ggcggcagag gctccggtag aagagaaagc ggtctggagt
     2041 tcctgcaccg ttaactgcgg gagccgctgt ctgttacgtt tgcatgtgaa agatgacacc
     2101 gtgtactggg tggagtctga tacgacaggt gacgacgtct acggtaatca tcaggttcga
     2161 gcgtgtttac gcgggcgctc tatccgccga cggatgaatc atcctgatag gttgaaatat
     2221 cccatgaagc gcgtcggcaa gtgcggtgaa ggtaaatttg aacggataag ttgggacgaa
     2281 gccctggata ccatcagtga taatcttcgg cggatcctga aagattacgg caatgaggct
     2341 gtacatgtcc tgtacggaac aggcgtagat ggcggaaaca tcaccaactc aaacgtcccg
     2401 taccgtctga tgaactcttg cggtggtttt ctcagtcgct atggcagcta cagtaccgca
     2461 cagatcagtg ccgcaatgag ttatatgttc ggtgccaatg atggcaacag cccggatgat
     2521 atcgccaata cgaaactggt cgttatgttc ggaaataacc cggcagaaac gcggatgagc
     2581 ggcggtggtg tcacttacta cgtcgagcaa gcccgcgaac gttcaaacgc acgcatgatc
     2641 gtcatcgatc cacgttataa cgacactgct gccgggcgtg aagatgaatg gctgcctatt
     2701 cgccctggca ccgatggcgc actggcctgt gcgattgcct gggtactgat tactgaaaac
     2761 atggtcgatc agccatttct cgacaaatat tgtgttggtt acgatgaaaa aacgctgccc
     2821 gccaacgcac cacgtaacac gcattataaa gcctatattc tgggcgaagg gcctgacggc
     2881 atagctaaaa cgccggaatg ggcagcaaaa atcaccagca tcccggcaga aaaaattatc
     2941 cagttggcgc gagagatcgg ttcagcaaaa cctgcttata tttgtcaggg ttgggggcca
     3001 caacgacatt ccaacggcga gcaaacgtcc cgcgctattg ccatgctttc cgttctcacc
     3061 ggtaacgttg gcataaacgg cggcaactca ggtgtacgcg aaggtagctg ggatctgggg
     3121 gtagaatggt tcccgatgct tgagaatcct gttaaaacgc agatttccgt ctttacatgg
     3181 acagatgcca tcgaccatgg tacggaaatg accgcgaccc gtgatggtgt tcgtggaaaa
     3241 gaaaaactgg atgtccccat caagttttta tggtgctacg ccagtaacac attgatcaat
     3301 caacatggcg acatcaatca cacccatgag gtgcttcagg atgacagcaa gtgcgagatg
     3361 attgttggca ttgaccactt catgacggcc tcggctaagt attgcgatat cctgttgccc
     3421 gacctgatgc caacagagca agaagacctt atctctcatg aatctgcagg gaatatgggc
     3481 tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg aacgcaaacc catctactgg
     3541 atgctgagtg aagtcgccaa acgcttagga ccagacgttt atcaaacctt tactgaaggt
     3601 cgcagtcagc atgaatggat caaatatctc catgcgaaaa cgaaggaacg taaccctgag
     3661 atgctcgact acgaggagat gaaaacgacc gggatcttta agaaaaaatg cccggaagaa
     3721 cactacgtcg ctttccgcgc attccgtgaa gatccacagg caaacccgtt gaaaacacct
     3781 tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga ttgcagatac ctgggaattg
     3841 aaaaaagatg aaattattca tccccttcct gcgtataccc caggttttga tggctgggac
     3901 gatcccctgc ggaaaaccta tccactgcag ttaacgggct tccactacaa agcgcgtacc
     3961 cactccagct acggcaatat tgatgtgtta cagcaggcct gcccacaaga ggtgtggatc
     4021 aaccccattg atgctcaggc acgcggtatc cgtcatggcg ataccgtgcg ggtatttaac
     4081 aataatggag aaatgctgat tgccgcaaaa gtgactccgc gtattctgcc tggcgttacc
     4141 gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg gtgaccgggt cgatcacggc
     4201 ggcagtatca atattctgac ctctcaccgc ccttcaccgc tggcaaaggg aaacccgtcg
     4261 cacagcaatc ttgtccagat cgaaaaggtt taaggagtag cccatgacca cacaatatgg
     4321 attttttatc gattccagcc gctgtactgg ctgtaaaacc tgcgaactgg ggtgcaaaga
     4381 ttttaaagat cttggcccgg aagtcagttt ccgccgtatt tatgaatatg ctggcggcga
     4441 ctggcaggaa gataatggca tctggcacca gaacgtgttc gcttattatc tctccatctc
     4501 ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc cccagcggcg caatgcataa
     4561 gcgtgaagat ggctttgtgg tggttgatga agatgtttgt atcggctgcc gctactgcca
     4621 catggcttgt ccgtacggcg caccacagta caatgctgaa aaagggcaca tgacgaagtg
     4681 cgatggttgt tattc
//
LOCUS       DAJVTY010000216         4658 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVTY010000216 DAJVTY010000000
VERSION     DAJVTY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.217"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1681)
                     /locus_tag="OQQ66_003820"
     CDS             complement(<1..1681)
                     /locus_tag="OQQ66_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS1906563.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDNDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQ"
     gene            complement(1681..2751)
                     /locus_tag="OQQ66_003821"
     CDS             complement(1681..2751)
                     /locus_tag="OQQ66_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1906564.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2741..4192)
                     /locus_tag="OQQ66_003822"
     CDS             complement(2741..4192)
                     /locus_tag="OQQ66_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS1906565.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4203..4649)
                     /locus_tag="OQQ66_003823"
     CDS             complement(4203..4649)
                     /locus_tag="OQQ66_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1906566.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAAPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1141 a         1245 c         1197 g         1075 t
ORIGIN      
        1 gctgacacaa tatgcttgcc agatagctaa atatttttag ctcatgacgc gcatcggcgg
       61 cggcgcaggc gatcaagaca tggttaatga cttcgttatt cacctcaact ggttgggcga
      121 gggtaataaa aaatccacga aaacgtcgct cctgttcgct ccagcaatgt gggatggcga
      181 ggcgattaac aatcaggttt tcaccttcgc cttcgcgggc gatgatgcgt tgtgcttcat
      241 cggcggtaat atggtgttgt gcgactaatt gcgcacaaat ttgccgggta atatgttgcc
      301 agctttcacc gcgtacattc gaataatgaa aacttccggg ggcagagaaa aaacgctccg
      361 gttgctggcg aataaatgcc gtcgccagaa aatgttttat ttgttcgata ccggcagccg
      421 taatgatatt ttttacggtg atgtaattat ttaccgcgtc ttccagtaaa taatggctat
      481 tgttaatgat caataacggc tcaatctctt cgcgaatggc aacaagttcg cttaagctac
      541 gggcaataat tacccgacaa tttaaaacat cgcgctcaat ggcgagctga ttaatagtgg
      601 caatcgcgtt ctggtccgag agcaaaatga tcggctggcg ttcgttttga tgccgctcca
      661 gcgcacaggc aaaatacaaa ccgatcagat cgttgtcgaa aaggggaata tcgagctgtt
      721 cacgcagtag cgtaataaag tgcagactca tatcaaaggc cgcaggccag gctgctttca
      781 ggttattcat gctactttgg cggtgctcag caatccacac cggtgcggca aggcaacgta
      841 ataaatgccc ggtaatattc tcaaccaact gcgtatcaat aatattcaat gacgcgatgt
      901 ctggcaccct ttgcagcaac cccagcacat gatcggctga aattacctgc gcttgctgat
      961 gctgtttctc tatcaaacct gtcagggttc ttacagcgtt atcaccaaga aacatgccgc
     1021 tttgttcaac gatttgtttt atctcgtcac cgggatattg cggccactca tcggtcagtt
     1081 gattacgcag ggcgtaaatc gccagaatca ggctggagag ataatcaccc tgcatgagcg
     1141 gccagcggtg ttggttgtcg caggccgtgg ataaatgctg aaggttgtct cgtgttacgc
     1201 ccgccagcgg aattaaaaac ggatctttgc gcagcaagtt cgccagcaag ataacgcgct
     1261 tctcttctgt ctcatcaatg aaatggccca aaccggggcg gctggccagg caacaagcgc
     1321 gttcataacg ctgttttaac cggggcagac gctctgcgac ccacgtttct ggcaaattaa
     1381 gcgccgaggc gagttgcgca cggggagtaa aggtattcag taataacagt gccagcagcc
     1441 gatagtcgtt atcgtgcttt tgcagcaact gaaaaaagct gcggcgctcg aagatttcca
     1501 gctgatagcc cgcactacca ctggcggaaa tgcgggcttt gccgttaagg gtaaagttga
     1561 gatagtcaat atcacgcagg atggtcctgc ctgaaacgcc agtctgttgc gccagctcac
     1621 caggcgtgcg cggctgttgc tccagcagat cgacaatctt taactggcgt tcgtttagca
     1681 ttaatccatc tgacggaact ggctaaacgc gttgactttc tccgcggtta aggtggtcag
     1741 aaaatcccgt atcaacgtga gcagagcatc gtaatcggct tttgaaatca taccgctgtt
     1801 agcgtgcaga tagcgggttg gcaggcacag cgcaacaacc ggacgcccgc cgcccatcac
     1861 gttgtagcgc ccgccatccg tcgcaccggt tttcatggtg gaaaattgca gcggtaaatc
     1921 gttatgtgcg gcacagcttt ttaacgctgc taccagtttc tggttgggga agtagcgctt
     1981 gtcaaacagc atcagccccg gcccctggcc cagtttcagc gggtatttaa tgttatcaat
     2041 gcccggaaca tcgcctgcga cagcggtatc cagcacgatc actacatccg gtttaatgtg
     2101 ttccgccgag gtttgtgccc cgcgtagccc aacttcttct tccacgctgc cgacgccata
     2161 cagcgtaatt tcgggattat tcaccgtctg caatagttca gccatcattg cgcagccgat
     2221 gcggttatcc agcgccttgc cgaccacttt atcttcgccc cagcaggcaa aattggcttc
     2281 cgggctaata aaatcgccca tcgccacacc gcgtttttcc acctcatcgc gactgtttgc
     2341 gccaatatca atgaacatct catcaaatga cagcggccgt tgcctttgct tttccgttaa
     2401 cgcatgaggc gcgacggaac caatcacacc agggattttc actcccttgt gtgtgcgtat
     2461 ggttacccgg tggttgagca tcgactgatt ccaccagcca ccaatggtgg tgaaacgcag
     2521 aaaaccgctc tcgtcgatgt gggtgaccat aaagccgact tcatccatat gcccgacaac
     2581 ggcaactttt ggacctttat tccccttacg ggcaacaaag ctgcccagac catcaaaggt
     2641 gatctcatta acgcaaggtt ccagcgtgtt tatcagaatg tcgcgaactt cctgttcatc
     2701 gccgctgacg gcgctggctt cgcacaactg ttgcagtaac tcaatgttca tggacaagct
     2761 cctgttgcgc ggctttcgct ttgcggcgta accacaatcc tttcaggacg ataatgaatg
     2821 aaatgttcag cgcgaggcca atcgccagca ccagataaaa cgaacccacc ggcgacatca
     2881 ggccgatcag cgggtcaaag atacccaaac caggtgccag acgtttgatt ccgaaagcga
     2941 tcaccagcac gccggtaatg ccacccgcca gcgtattggc ggtaatcatc ggcaacggag
     3001 ctgccagagc gtaaggtata gccggttcgg ttgcgactgt ggcaccgacc acaatggcac
     3061 tactggcagc ctctttttcc tgtcgggtaa acagtttggg cgcaatgaac gtcgcaagac
     3121 ccgccgcaac gggcggcatc aacgcgacaa cgccgacgat ggcgtaccag tcataaatgt
     3181 gtttttccag cagtgagaag cagaagaacc aggcggtttt attgatgggg ccgcccatat
     3241 cgaacgccag catcgcaccg accagaaacg ctgctgcaaa cttcattgat ggaggaatgg
     3301 tattcaggaa gtggagcagc ccgcccatga ggtcggacat caccggcccg ataacgtagt
     3361 aagtgagcac gccgaaaacc agcagagtaa cgaacgggat cagcattgaa ccgagcagcg
     3421 gttgtaatgc tttgccaagg cgcactttgc ggaaccagaa gacgaaatag ccaatcgcca
     3481 gccccagcac caccgcgccg aggaatcccg cacccgactg ggtgccgagc agcgctttgt
     3541 cgttcgccag atagcaaacc agaaacgccg gagcgaaagc cggtttatcg gcaatcgacg
     3601 aggcaatata agcgcccatg atggggatca taaaggtgaa gccaaggtaa ccgatggatt
     3661 caactaccca ggtaaacgac ggcgcgcctt tcgacatatc ggtatacggc aaaccgaact
     3721 gcaccagcat gttggcaagc gccaccagga taccaccgcc gatgacaaac ggcagcgccg
     3781 cagagacgcc cgccatcagg tggctcatca cgctgccgct ttgcatttcc tgtttgccaa
     3841 gcttcacgcc actatctgcg gcaaaaagct gcgagtttgt cggtaattcg ctgaaaatct
     3901 ggtcgatatt tttcaacgcc tgggagatgg caatctcata aactttcttc ccggtaaacc
     3961 gcgcgcgatc atcaccgctc aggccacgcc cggtagcgag aatgacgtaa tcggcagcgg
     4021 caatctcttc gctggataag cggttttcaa cgccactgga cccttgagtt tccactttta
     4081 tggtatggcc gagagaacgg gctttctgtt ccagggcttc cgccaccatg taggtgtgag
     4141 cgatcccggc ggggcagttg gtgatcgcga caatacgtaa ggatgactcc atcgccactt
     4201 ccttatgagc tgagggtttg atttaacaag gcaagcactt ggtcggtatc gccctgttgc
     4261 agttgatgaa taaattcctt gtgaataatt ttgcgacaca gtgtgccgat gattttgacc
     4321 tgatcctctt cgccgctttg cggcacgccg aggcagatcc agcaattgac gtcttcgcca
     4381 tcgctggctt tccagtcaat agcctgcgct ttgcgggcga ataatacgaa cggttgttta
     4441 acgcaggcgc ttttaccgtg cggcgcggcg acaccggaac caaatccagt agagtgcatt
     4501 ttttcgcgca gcagcagggt ttgcaggaac tggtgtgagt cggtgataaa accgttttgt
     4561 aacgctattg tcgccaactg cttcagaacg gaataagcgc cattgccctg aatattcagg
     4621 tcaatacagc ttgcagtaag agctgccatt aatcggac
//
LOCUS       DAJVTY010000217         4644 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVTY010000217 DAJVTY010000000
VERSION     DAJVTY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.218"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(18..2297)
                     /gene="maeB"
                     /locus_tag="OQQ66_003824"
     CDS             complement(18..2297)
                     /gene="maeB"
                     /locus_tag="OQQ66_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1906567.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2586..3536
                     /gene="tal"
                     /locus_tag="OQQ66_003825"
     CDS             2586..3536
                     /gene="tal"
                     /locus_tag="OQQ66_003825"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1906568.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMAPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3556..>4644
                     /locus_tag="OQQ66_003826"
     CDS             3556..>4644
                     /locus_tag="OQQ66_003826"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1906569.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT         1138 a         1242 c         1188 g         1076 t
ORIGIN      
        1 tgagagttaa aaaagaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc
       61 atgttgacga tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg
      121 cccatcagca ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa
      181 ctaatgcggg cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg
      241 ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca
      301 ccatcaatca tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg
      361 ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggc
      421 tcaataccaa aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct
      481 gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc
      541 agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc
      601 acgctaaaat gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca
      661 cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcccg ctgcgcctgt
      721 tcctgagtga caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag
      781 cgcggatcgg attcgttatt gacgatctca aaatcaacgc ccgctttgat ctgcaagccc
      841 agtttctgaa tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt
      901 cccagcgtta ccagttcctg agtggcatgc agaacgcgcg cctcttcccc ttctggcaga
      961 acaacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt
     1021 ttgtaaacga actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc
     1081 gccacgcccg actccatcgc ggctttagcg accgcaggag cgatcttaac gatcaagcgc
     1141 ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg atcgccatac
     1201 gctgaagcca ccacttcgct ctgttccgca tgggcgagtt ctgcaatcgc acgtaccgcc
     1261 gccagtttca tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg
     1321 aacgggaagc acaggacgtt gttcacctgg ttcggatagt cagaacgacc ggtgcaaatg
     1381 atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc
     1441 agcgccagga tcattggcgc acgagccatt ttcttcacca tttcctgggt cagcactttc
     1501 gggccggaac atccgaggaa aatatccgcg ccttcaatca catcatcgag ggtacgtttg
     1561 ccgtcgtcca ccaccgcata tgcggctttg gtttccgcca tgtttggctc acggccctga
     1621 tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagacc cagcgctacc
     1681 agcaggttca tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg
     1741 gagatgtttt tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc
     1801 gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca
     1861 atatagaaac attctggcgc tttaatgtct tcgaggttga ttccgccgaa ggtcggttcg
     1921 agcgcagcga caacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat
     1981 acatcaatcc cggcgaattt cttaaacaga acgcccttgc cttccatcac cggtttgcca
     2041 gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat caccgccacc
     2101 aggttaccgc gcgcggtata tttgtaggct tttaacgggt ctttttcgat ttcaagacaa
     2161 ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg
     2221 gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt
     2281 tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg
     2341 gcaacagccc ctgcccacaa cgtggcgcac attattaccc cgccggagtc tacagacttt
     2401 gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta taacactgat
     2461 gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc
     2521 gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat
     2581 ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagcggcg
     2641 atattgagtc cattcgccat tatcatcccc aggatgccac caccaatcct tcgctgttgc
     2701 tcaaggctgc cggattatca caatatgagc atttaataga cgatgctatc gcctggggta
     2761 aaaaaaatgg caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca
     2821 atttcggtgc tgaaattctc aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac
     2881 gcctctcttt tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc
     2941 agcaacaagg cgttgagaaa tcacgcattc tgatcaaact ggcttcgacc tgggaaggaa
     3001 ttagcgcggc agaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt
     3061 cttttgccca ggcgcgggcc tgtgcggaag caggcgtttt tctgatttcg ccgtttgtcg
     3121 ggcgtattta tgactggtat caggcacgca agccgatggc cccgtatgtg gtggaagaag
     3181 atccgggcgt taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa
     3241 ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg
     3301 atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tcgccagtgg
     3361 tacgtaaatt aatcccacct tctcagacgt tcccacgccc agctcccatg agcgaagcgg
     3421 agttccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc
     3481 gtctgttcgc cgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca
     3541 gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg
     3601 gatgcggtac aaaaagccaa ctctggtcat cccggcgcgc cgatgggcat ggctgatatt
     3661 gccgaagtgc tgtggaacga ttttcttaaa cataacccta ccgacccaac cgggtatgat
     3721 cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctgcat
     3781 ctgaccggtt acgacctgcc gctggaagag ctgaagaact tccgtcagtt gcattcgaaa
     3841 accccaggcc acccggagat tggttatacg cccggtgttg aaaccaccac cggtccactt
     3901 ggacaaggtt tggcgaacgc cgtcggactg gcgatagcag agcgtacact ggcggcgcag
     3961 tttaatcagc cggatcatga aattgtcgat cacttcacct atgtgtttat gggcgacggc
     4021 tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg cgggcacgct aggactgggc
     4081 aagctgattg gtttttacga tcacaacggt atttcgattg atggagaaac cgaaggctgg
     4141 tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc
     4201 gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat
     4261 aagccgtcgc tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt
     4321 aaggaagagg cgcacggcgc accactgggg gaagaagaag tggcgctggc acggcaaaaa
     4381 ctgggctggc accatccgcc atttgagatc cctaaagaga tttatcacgc ctgggatgcc
     4441 cgtgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag
     4501 gctcatccgc aactggcaga agagtttacc cgacggatga gcggtggttt accgaaggac
     4561 tgggagaaaa cgactcagaa atatatcaat gagttacagg caaatccggc gaaaatcgct
     4621 acccgtaagg cttcgcaaaa tacg
//
LOCUS       DAJVTY010000218         4548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVTY010000218 DAJVTY010000000
VERSION     DAJVTY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.219"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            54..1853
                     /gene="cysJ"
                     /locus_tag="OQQ66_003827"
     CDS             54..1853
                     /gene="cysJ"
                     /locus_tag="OQQ66_003827"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1906570.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1853..3565
                     /gene="cysI"
                     /locus_tag="OQQ66_003828"
     CDS             1853..3565
                     /gene="cysI"
                     /locus_tag="OQQ66_003828"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1906571.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3640..4374
                     /gene="cysH"
                     /locus_tag="OQQ66_003829"
     CDS             3640..4374
                     /gene="cysH"
                     /locus_tag="OQQ66_003829"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1906572.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1119 a         1188 c         1307 g          934 t
ORIGIN      
        1 atgggcgctg acagggcgca gaaacagctt tgcttactgg aacataacga cgcatgacga
       61 cacaggtccc accttccgcg ttgcttccgt tgaacccgga gcaactggca cgccttcagg
      121 cggccacgac cgatttaacg cctacccagc ttgcctgggt ttctggctat ttctggggcg
      181 tactcaatca gcagcctgct gcgcttgcag cgacgccagc gccagccgca gaaatgccgg
      241 gtataactat tatctccgcc tcgcaaaccg gcaatgcgcg ccgggttgct gaagcattac
      301 gcgatgattt attagcagca aaactgaacg ttaagctggt gaacgcgggc gactataaat
      361 tcaaacaaat cgccagcgaa aaactgctca tcgtagtgac gtcaacgcaa ggggaagggg
      421 aaccgccgga agaagccgtc gcgctgcata agttcctgtt ctccaaaaaa gcgccaaagc
      481 tggaaaacac cgcgtttgcc gtgtttagcc tcggcgatag ctcttatgaa tttttctgcc
      541 agtccgggaa agatttcgac agcaagctgg cggaactggg tggtgaacgc ctgctcgacc
      601 gtgtcgatgc cgatgttgaa taccaggctg ctgccagcga gtggcgcgcc cgcgtggttg
      661 atgcgcttaa atcgcgtgcg cctgtcgcgg caccttcgca atccgtcgct actggcgcgg
      721 taaatgaaat ccacaccagc ccgtacagca aagacgcgcc gctggtggct agcctctctg
      781 ttaaccagaa aattaccggg cgtaactctg aaaaagacgt tcgccatatc gaaattgact
      841 taggtgactc gggcctgcgt taccagccgg gtgacgcgct gggcgtctgg tatcagaacg
      901 atccggcact ggtgaaagaa cttgtcgaac tgctgtggct gaaaggcgat gaacctgtca
      961 ccgtcgaggg caaaacgttg cctctgaacg aagcgctaca gtggcacttc gaactgaccg
     1021 tcaacaccgc caacattgtt gagaattacg ccacgcttac ccgcagcgaa acactgctgc
     1081 cgctggtggg cgataaagcg aagttacagc attacgccgc gacgacgccg attgtcgaca
     1141 tggtgcgttt ctctccggcg caactggatg ccgaagcgct gattaatctg ctgcgcccgc
     1201 tgaccccgcg cctgtattcc atcgcctcct cgcaggcgga agtcgagaac gaagtacacg
     1261 tcaccgttgg tgtggtgcgt tacgacgtgg aaggccgagc ccgtgccggt ggtgcctcca
     1321 gcttcctcgc ggatcgcgtg gaagaagagg gcgaagtccg cgtatttatc gaacataacg
     1381 ataactttcg cctgcccgct aacccggaaa ccccggtgat tatgattggc ccaggcaccg
     1441 gtattgcgcc gttccgcgcc tttatgcagc aacgcgccgc cgacgaagcg ccaggtaaaa
     1501 actggctgtt ctttggtaat ccgcacttta cggaagactt cctgtaccag gtggagtggc
     1561 agcgctacgt caaagatggc gtgctgacac gtatcgatct tgcctggtcg cgcgaccaaa
     1621 aagaaaaagt ttacgtacaa gacaaactgc gcgaacaggg cgcggagctg tggcgctgga
     1681 tcaatgatgg tgcccacatt tatgtctgcg gcgacgctaa tcgcatggcg aaagacgttg
     1741 agcaggcact tctggaagtg attgccgaat ttggtggcat ggacatcgaa gcggcggatg
     1801 aatttttaag tgagctgcgc gtagagcgcc gttatcagcg agatgtctac taatgagcga
     1861 aaaacatcca gggcctttag tggtcgaagg aaaactgaca gacgccgagc gcatgaagct
     1921 tgaaagcaac tacctgcgcg gcaccattgc ggaagattta aacgacggtc tgaccggcgg
     1981 ctttaagggc gacaacttcc tgctgatccg cttccacggc atgtatcagc aggatgaccg
     2041 cgacatccgc gccgaacgtg ctgaacagaa gctggagccg caccacgcga tgctgcttcg
     2101 ctgtcgtctg ccgggtgggg tgattaccac taaacagtgg caggcgatcg acaaatttgc
     2161 cggtgaaaac accatctatg gcagcattcg cctgaccaac cgccagacat tccagttcca
     2221 cggcattctg aaaaagaacg tcaaaccggt gcaccagatg ctgcactcgg tcggtcttga
     2281 tgcgctggcg acagctaacg acatgaaccg taacgtactc tgcacctcga acccttacga
     2341 gtcgcagctg cacgcggaag cgtacgagtg ggcgaagaag atttctgagc atctgttgcc
     2401 tcgtacccgc gcgtatgcgg agatctggct cgaccaggaa aaagtcgcca ctactgatga
     2461 agaaccgatc cttggtcaga cctacctgcc gcgtaagttc aaaaccacgg tagtgatccc
     2521 gccgcagaac gatatcgatc tgcatgccaa cgacatgaac ttcgtggcaa tcgccgaaaa
     2581 cggcaagctg gtaggcttca acctgctggt gggcggtggg ctttctatcg aacacggcaa
     2641 caagaaaacc tacgcccgca cggcgagcga gtttggctat ctgccgctgg agcatacgct
     2701 ggcggtggcc gaagctgtcg tgacaactca gcgtgactgg ggtaaccgaa ccgatcgtaa
     2761 aaatgccaaa accaaataca cgctggagcg cgtgggggtt gagacgttta aagcggaagt
     2821 ggagcgtcgc gcggggatca aatttgaacc gatccgtcca tatgagttca ccggacgagg
     2881 cgatcgtatt ggctgggtta agggcattga tgataactgg cacctgacgc tgtttatcga
     2941 aaatggtcgc atccttgatt atccggggcg tccgctgaaa accggcctgc tggagatcgc
     3001 gaagatccac aaaggcgatt tccgcattac ggcgaaccag aatctgatca tcgccggtgt
     3061 accggaaagc gagaaagcga agatcgagaa gatcgccaaa gagagcgggt taatgaatgc
     3121 cgtcacgccg cagcgtgaaa actcgatggc ctgcgtgtca ttcccgactt gcccgctggc
     3181 gatggcggaa gcagagcgtt tcctgccgtc ttttatcgac aacatcgata atttaatggc
     3241 gaaacatggt gtcagcgatg agcatatcgt gatgcgtgta acaggctgcc cgaacggttg
     3301 tggtcgcgcg atgctggcgg aagtgggcct ggtgggtaaa gcgccgggtc gctacaacct
     3361 gcatcttggc ggcaaccgca ttgggacacg tatcccacgg atgtataaag aaaacatcac
     3421 cgagccggaa atcctggcgt cgcttgatga actgataggg cgctgggcga aagagcgcga
     3481 agcgggtgaa ggcttcggcg actttacggt gcgtgcgggc atcattcgcc cggtgctcga
     3541 tccggcgcgc gatttgtggg attaaccatc agcccggtct tgtaggcctg ataagaacgc
     3601 gtgagcgtcg catcaggcaa ggcaaacagt gaggaatcta tgtccaaact cgatctaaac
     3661 gccctgaacg aactgccgaa ggtagatcgc attctggcgc tggcggaaac taacgccgaa
     3721 ctggaaaaac tggacgctga aggccgcgta gcctgggcgc tggataatct gcccggtgaa
     3781 tatgtacttt cttccagctt tggcattcag gcggcggtga gcctgcatct ggtgaatcaa
     3841 attcgcccgg atattccggt gatcctcacc gatacgggtt acttgttccc ggaaacctac
     3901 cgctttattg acgagttaac ggacaaactc aagctcaacc tgaaagtgta ccgtgctacc
     3961 gaaagcgcag cctggcagga agcacgctac ggaaaactgt gggagcaggg cgttgaaggc
     4021 attgaaaagt acaatgacat caacaaagtc gaaccgatga accgggctct gaaagaactt
     4081 aacgcgcaaa cctggtttgc tggcctgcgc cgtgaacaat ccggcagtcg cgccaattta
     4141 ccggtgctgg caattcagcg tggcgtattt aaagtgctgc cgattatcga ctgggataac
     4201 cgaactattt atcagtacct gcaaaaacat ggcctgaaat atcacccatt atgggatgaa
     4261 ggatatttat cggtcggtga tacccataca acccgtaaat gggaacctgg catgtcggaa
     4321 gaagaaacac gtttctttgg cttaaaaagg gaatgtggat tgcacgaagg gtaaatattg
     4381 ccggataaat taaacgcctc tgtcagaaat gatgggggcg tttttatttt gcgagcagta
     4441 ttcctgattt taaacgtgaa cgccgctgga agaattatca ggtatagaat ggcgtcgggt
     4501 gcttgaggct gtctggcgtt aagcattcgc gaggttccag atggacaa
//
LOCUS       DAJVTY010000219         4511 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVTY010000219 DAJVTY010000000
VERSION     DAJVTY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.220"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..909)
                     /locus_tag="OQQ66_003830"
     CDS             complement(<1..909)
                     /locus_tag="OQQ66_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCS1906573.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGI"
     gene            1188..2141
                     /gene="tal"
                     /locus_tag="OQQ66_003831"
     CDS             1188..2141
                     /gene="tal"
                     /locus_tag="OQQ66_003831"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1906574.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2256..2843
                     /gene="mog"
                     /locus_tag="OQQ66_003832"
     CDS             2256..2843
                     /gene="mog"
                     /locus_tag="OQQ66_003832"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1906575.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2878..3444)
                     /gene="satP"
                     /locus_tag="OQQ66_003833"
     CDS             complement(2878..3444)
                     /gene="satP"
                     /locus_tag="OQQ66_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1906576.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3593..4306)
                     /gene="msyB"
                     /locus_tag="OQQ66_003834"
     CDS             complement(3593..4306)
                     /gene="msyB"
                     /locus_tag="OQQ66_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1906577.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4332..>4511)
                     /locus_tag="OQQ66_003835"
     CDS             complement(4332..>4511)
                     /locus_tag="OQQ66_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1906578.1"
                     /translation="ARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
BASE COUNT         1179 a         1167 c         1137 g         1028 t
ORIGIN      
        1 aatgccaatc atctggacaa tcccttgcgc tgccggatgc ggaggccagg acgccgctgc
       61 cgctgccgcg tttggcgtcg aacccattcc cgcctcattg gaaaacatac tgcgctgaaa
      121 accgttagta atcgcctggc ttaaggtata tcccgccgcg ccgcctgccg cttcctgcca
      181 gccaaaagca ctctcaaaaa tagaccaaat gacgtgggga agttgcccga tatttattac
      241 gcaaatcacc atgctggtca gcacccagat tatcgccatc aacgggacaa acccctgcat
      301 gagccgggcg acgccatgaa gaccgcgagt gattgccagc agagcaaaga cagcgagaat
      361 aatgcctgtc accagcgggg gaaaatcaaa agaaaaactc agggctcggg caacggcgtt
      421 cgcctgaact ccgctgaaaa ttatgccgta ggcgatgagc aaaaagacgg cgaacagaac
      481 gcccatccaa cgcatcccca gcccgcgcgc catataccat gccggtccgc cacgaaactg
      541 cccattgacg tcacgttctt tataaagttg tgcaagggaa cattcggcaa acgaggtcgc
      601 catgccgata aacgcggcaa cccacatcca aaagacggct ctaggtccac cggcggtaat
      661 agccagcgca acgccggcca ggttgccgct acccacgcgc gccgcaagac tggtacacaa
      721 tgactgaaat gaggttaaac cgcctggctg tggatgaatg ctatttttaa gacttttgcc
      781 aaactggcgg atgtagcgaa actgcacaaa tccggtgcga aaagtgaacc aacaacctgc
      841 gccgaagagc aggtaaatca ttaccgatcc ccaaaggacg ctgttaataa aggagaaaaa
      901 atctggcatg catatccctc ttattgccgg tcgcgatgac tttcctgtgt aaacgttacc
      961 aattgtttaa gaagtatata cgctacgagg tacttgataa cttctgcgta gcatacatga
     1021 ggttttgtat aaaaatggcg ggcgatatca acgcagtgtc agaaatccga aacagtctcg
     1081 cctggcgata accgtcttgt cggcggttgc gctgacgttg cgtcgtgata tcatcagggc
     1141 agaccggtta catcccccta acaagctgtt taaagagaaa tactatcatg acggacaaat
     1201 tgacctccct tcgtcagtac accaccgtag tggccgacac tggggacatc gcggcaatga
     1261 agctgtatca accgcaggat gccacaacca acccttctct cattcttaac gcagcgcaga
     1321 ttccggaata ccgtaagttg attgatgatg ctgtcgcctg ggcgaaacag cagagcaacg
     1381 atcgcgcgca gcagatcgtg gacgcgaccg acaaactggc agtaaatatt ggtctggaaa
     1441 tcctgaaact ggttccgggt cgtatctcaa ctgaagttga tgcgcgtctt tcctatgaca
     1501 ccgaagcgtc aattgcgaaa gcaaaacgcc tgatcaaact ctacaacgat gctggtatta
     1561 gcaacgatcg tattctgatc aaactggctt ctacctggca gggtatccgt gctgcagaac
     1621 agctggaaaa agaaggcatc aactgtaacc tgaccctgct gttctccttc gctcaggctc
     1681 gtgcttgtgc ggaagcaggc gtgttcctga tctcgccatt tgttggccgt attcttgact
     1741 ggtacaaagc gaatactgat aagaaagagt acgctccggc agaagatccg ggcgtggttt
     1801 ctgtatctga aatctaccag tactacaaag agcacggtta tgaaaccgtg gttatgggcg
     1861 caagcttccg taacatcggc gaaattctgg aacttgcagg ctgcgaccgt cttaccatcg
     1921 cacctacact gctgaaagag ctggctgaga gcgaaggggc tatcgaacgt aaactgtctt
     1981 acaccggcga agtgaaagcg cgtccggcgc gtatcactga gtccgagttc ctgtggcagc
     2041 acaaccagga tccaatggca gtagataaac tggcggaagg tatccgtaag tttgctattg
     2101 accaggaaaa actggaaaaa atgatcggcg atctgctgta atcattctta gcgtgaccgg
     2161 gaagtcggtc acgctacctc ttctgaagcc tgtctgtcac tcccttcgca gtgtatcatt
     2221 ctgtttaacg agactgttta aacggaaaaa tcttgatgaa tactttacgt attggcttag
     2281 tttccatctc tgatcgcgca tccagcggcg tttatcagga taaaggcatc cctgcgctgg
     2341 aagaatggct gacatcggtg ctaaccacgc cgtttgaact ggaaacccgc ttaatccccg
     2401 atgagcaggc gatcatcgag caaacgttgt gtgagctagt ggatgaaatg agttgccatc
     2461 tggtgctcac cacgggcgga actggcccgg cgcgtcgtga cgtaacgccc gatgcgacgc
     2521 tggcagtagc ggaccgcgag atgcctggct ttggtgaaca gatgcgccag ataagcctgc
     2581 attttgtacc aactgcgatc ctttcgcgtc aggttggtgt gattcgcaaa caggcgctga
     2641 tccttaactt acccggtcag ccgaagtcta ttaaagagac gctggaaggt gtgaaggacg
     2701 ctgcgggtaa cgttgtggta cacggtattt ttgccagcgt accgtactgc attcagttgc
     2761 tggaagggcc atacgttgaa acggcaccag aagtggttgc tgcattcaga ccgaagagtg
     2821 caagacgcga cgttagcgaa taaaaaaatc cccccgagcg gggggatctc aaaacaatta
     2881 gtgggattca ccaatcggca gaacggtgcg accaaactgc tcgttcagta cttcacccat
     2941 cgccagatag attgcactgg caccacagat cagaccaatc cagccggcaa agtggatgat
     3001 tgcggcgtta ccggcaatgt taccgatcgc cagcagggca aacagcacag tcaggctaaa
     3061 gaaaacgaat tgcagaacgc gtgcgccttt cagcgtgccg aagaacataa acagcgtaaa
     3121 tacgccccac agacccaggt agacaccaag gaactgtgca tttggcgcat cggtcagacc
     3181 cagtttcggc atcagcagaa tcgcaaccag cgtcagccag aaagaaccgt aagaggtgaa
     3241 tgcggttaaa ccgaaagtgt tgcctttttt gtactccagc agaccagcaa aaatttgcgc
     3301 gatgccgctg tagaaaatgc ccatggcaag aataataccg tccagagcga aataacccac
     3361 gttgtgcagg ttaagcagaa tggtggtcat gccgaagccc atcaggccca gcggtgccgg
     3421 attagccaac ttagtgttgc ccataattcc tcaaaaatca tcatcgaatg aatggtgaaa
     3481 taatttccct gaataactgt agtgttttca gggcgcggca taataatcag ccagtggggc
     3541 agtgtctacg atcttttgag gggaaaatga aaattttccc cggtttccgg tatcagacct
     3601 gagtggcgct aaccatccgg cgcaggcagg cgatttgcag tacggctgga atcgtcacgc
     3661 gataggcgct gccgctgacc gctttaaccc catttagtgc cgcacctaca gggcctccca
     3721 gccccgcgcc gcgcagcaaa ccatgcccaa gtacgctcat tgctgcgtgg gtgcgtaaaa
     3781 tgcgggtcag ttggctggaa agcaaatgcg acacgccttt tgccaataat ttgtctttca
     3841 tcagcagcgg cagcagctct tccagctcat tcaccctcgc atcgaacgcg tgcagaaact
     3901 cctgcttatg ttcctcgtcc attttcttcc aggtattacg cagaaattgt tccagtaact
     3961 gttgctcaat ttcaaacgta gacatctctt tgtcggcttt cagcttcaat cgttttgaaa
     4021 catcgagcaa aatggcccga tacaatttac cgtgtccgcg cagtttgttg gcgatactat
     4081 cgccaccaaa atgctgtaat tctccagcaa tcagctgcca gttgcggcga tgttgctcgg
     4141 gatgcccttc catcgattta aacagttcgt tgcgcatcag tacgctggag aggcgagttt
     4201 tgcctttttc attatgggtg agcaatcggg cgaaatttgc caactgttcc tcactacaat
     4261 gctgaagaaa atccagatct gaatcattca ggtaattaac attcattttt tgtggcttct
     4321 atattctggc gttagtcgtc gccgataatt ttcagcgtgg ccatatccga tgagttcacc
     4381 gtatgaccgg aaaaggtgat ttttgagacg cagcgtttat tgtcgttatc actgttaatg
     4441 ttgatccagt cagtggtttg cccttctttt atttctgaag gaatattcag actctgactg
     4501 gcgctacggg c
//
LOCUS       DAJVTY010000220         4480 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVTY010000220 DAJVTY010000000
VERSION     DAJVTY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.221"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            243..605
                     /gene="queD"
                     /locus_tag="OQQ66_003836"
     CDS             243..605
                     /gene="queD"
                     /locus_tag="OQQ66_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1906579.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            683..1951
                     /locus_tag="OQQ66_003837"
     CDS             683..1951
                     /locus_tag="OQQ66_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1906580.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1942..2202
                     /locus_tag="OQQ66_003838"
     CDS             1942..2202
                     /locus_tag="OQQ66_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1906581.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2219..2794
                     /locus_tag="OQQ66_003839"
     CDS             2219..2794
                     /locus_tag="OQQ66_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS1906582.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2942..3802)
                     /locus_tag="OQQ66_003840"
                     /pseudo
     CDS             complement(2942..3802)
                     /locus_tag="OQQ66_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(3799..>4480)
                     /locus_tag="OQQ66_003841"
     CDS             complement(3799..>4480)
                     /locus_tag="OQQ66_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1906583.1"
                     /translation="SLLRSLLGADEQAAAALLLAQRKNGTPMSLTALSMGDERALHWL
                     RYLMALGFEEAVLLETAADLRFAPEFVARHIAEWQHQNPLDLIITGSQSSEGQNGQTP
                     FLLAEMLGWTCFTQVERFTLDALFITLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPV
                     PGMRQRMAAGKAEIIRKTVAAEMPAMQCLQLARAEQRRGATLIDGQTVAEKAQKLWQD
                     YLRQRMQP"
BASE COUNT         1035 a         1200 c         1143 g         1102 t
ORIGIN      
        1 aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta
       61 acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa
      121 tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata
      181 atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta
      241 tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc ttaccacacg
      301 tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa
      361 ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa ctaaaagcgg
      421 cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt ccaggtctgg
      481 aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc
      541 cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc tatcgcggcg
      601 aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtattgtg
      661 ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg
      721 tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg ttttgttact
      781 ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat atacccatgc
      841 actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac gcatcactca
      901 cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct tacagcccgg
      961 cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg ccgaagccga
     1021 aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg aagaaaacgg
     1081 cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg tggtgctggc
     1141 agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc ctgctggcga
     1201 agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta ttgaagaacg
     1261 ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa tctgtgatga
     1321 cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag ggattgtttg
     1381 cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga ctcgctttaa
     1441 agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt atggtgcgca
     1501 tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta acggctggca
     1561 gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc gcggcatggg
     1621 tatggcgctg actggcgcgc aggcggcaca aacgctgata agcgcctgcc agcaccgcga
     1681 gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc tatgggatgt
     1741 tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctagctggt atcgggcgtg
     1801 gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata aacctgttcc
     1861 accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc atctggcggg
     1921 cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt tgctgatgcg
     1981 ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt gattaacgcc
     2041 tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga ttatcacggc
     2101 tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca acagtggcgc
     2161 tatccgcctt ccggattcgg catcacctac cgctttggat aaataataag gataatttat
     2221 gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag ataatgccag
     2281 cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg gcaatatctg
     2341 caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt ttattcatgt
     2401 tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga aactggtgac
     2461 cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa gagcggaagg
     2521 ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca acattgataa
     2581 gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta tgcccaaagt
     2641 gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc tggtgtgcga
     2701 tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac
     2761 cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc aattgaattt
     2821 ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg acgcttgacg
     2881 cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat ccggcaacac
     2941 attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact ttccagtcat
     3001 caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga ttaatcgcca
     3061 ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccggaa gcgccaacaa
     3121 caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt tcagcatcaa
     3181 cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc ttttctgcca
     3241 gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc acccgtctgg
     3301 cctcagctaa tgggtcgcga gtaacatttt tcagatccta tgtgctaacc agccagtcgg
     3361 gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg gcgtttttcg
     3421 ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt tgcagcgtcg
     3481 ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata tctaacgaag
     3541 tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac agttcatcgc
     3601 caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac tgttccacca
     3661 acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag tgcgccaggg
     3721 tacaaccact gaaatcctgc gccgccagcc agcttgctat tgcggcattc tcctgattta
     3781 ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct gccatagctt
     3841 ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc
     3901 tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt tacggataat
     3961 ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg ccacttcacc
     4021 gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca acccatgttc
     4081 agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca cctgggtgaa
     4141 gcaggtccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct gcccttcgct
     4201 gctttggctg ccggtaatta tcagatccag cggattctga tgctgccatt cggcaatatg
     4261 gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca atacagcttc
     4321 ctcaaaccca agagccatga gatagcgcag ccagtgcaat gcccgttcat cccccatgct
     4381 caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca gcgcggcagc
     4441 ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa
//
LOCUS       DAJVTY010000221         4467 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVTY010000221 DAJVTY010000000
VERSION     DAJVTY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.222"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..570)
                     /locus_tag="OQQ66_003842"
     CDS             complement(<1..570)
                     /locus_tag="OQQ66_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCS1906584.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLT"
     gene            complement(701..2065)
                     /gene="sdaA"
                     /locus_tag="OQQ66_003843"
     CDS             complement(701..2065)
                     /gene="sdaA"
                     /locus_tag="OQQ66_003843"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1906585.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2249..2827)
                     /locus_tag="OQQ66_003844"
     CDS             complement(2249..2827)
                     /locus_tag="OQQ66_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1906586.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(2831..4192)
                     /gene="pabB"
                     /locus_tag="OQQ66_003845"
     CDS             complement(2831..4192)
                     /gene="pabB"
                     /locus_tag="OQQ66_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1906587.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4266..4445
                     /locus_tag="OQQ66_003846"
     CDS             4266..4445
                     /locus_tag="OQQ66_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1906588.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1116 a         1153 c         1162 g         1036 t
ORIGIN      
        1 cgtcaggctg gcgctactga tttgctgcag ttgtggttca aggagcattg ccgtcagtaa
       61 gtcgatgtta atcatctcca tgaccccagc attgctactg cccgctgcag gcgtccattg
      121 aatcaaaacc ggactgcctt taatcagggc acggtcgatc gtcaggcgta aaagtggttg
      181 cggtgcagga agttcagcca gaatgtccac gacgggcaca ttgcgataac caaaaatgct
      241 ggagcaatag agtgtgccgt cttgcaccag gccaatggat cgcacagttt ggagttttgc
      301 cgcctgttta cgtaatggca aatgggcgac agagcagggc agaccaatca gcggaagcaa
      361 gacttcgcta cctgcctgta ggggaagcag tactttatct aattcctcta cagcgtgatt
      421 ggcgaattgt actacccgtt gttgatttaa gttacgctgt gaaataaatc gcacgctcag
      481 ggtcgaagcg agcgtaacga gtgcgcaaat cgtacaaaca atcattcgat tacgtcgata
      541 ggttttaatg atccgttgtg ctttttgcat gcgggccacc ctgtaagcgt atggcgacaa
      601 acgcctgacg caacagtgga tgtgtagtgg ggaatattac agcagacgag aaagcgggta
      661 taaattcgcc catccgttgc agatgggcga gtaagaagta ttagtcacac tggactttga
      721 ttgccagacc accgcgtgag gtttcgcggt atttggcgtt catgtcctta ccggtttcgt
      781 acatcgtttc gatgacctta tccagcgaga cgcgcggtgc actggtgcgg cgcagagcca
      841 tccgtgcggc gttaatcgcc ttcacagagg caatggcatt acgctcaatg cacggcacct
      901 gaacctgccc tgcaaccggg tcgcaggtta aaccaaggtt gtgttccatg ccaatttccg
      961 ccgccacgca aacctgttcc gggctaccgc ccagcagttc tgcaagaccc gcagcagcca
     1021 ttgaacaggc aacacccact tcgccctggc aaccaacttc cgcaccggaa atagaggcgt
     1081 tcattttata caatgcacca atcgcgcccg ctgccataaa gtaacgggta tagatgtccg
     1141 ggctgaccga ttcaataaag tggtcatagt aagccagcac tgccggaacg ataccgcagg
     1201 caccgttggt tggcgcagtt accacacgac caccggcggc gttttcttcg ttaactgcca
     1261 gcgcaaacat gtttacccag tcaatgacat tcatcggatc gttagacagt ttatcgctgg
     1321 aaaccagcat ccggcgcagg gcagacgcac gacgtggcac gcgcagcggg cctggcagta
     1381 caccttcggt gttcatcccg cgatcgatac atgcctgcat ggtttgccag acatgcgcga
     1441 aatactcgtc gatctctttc ttgctgtgca gcgccagttc gttctgcata gcgagaccag
     1501 acagcgaata gccggtttca ttacagtacg cgagcagttc ggtggcagat ttgaacggat
     1561 acggcacgct cacttcgttg gcagcatcct gaccaaagtg ttcttcatcg acgataaaac
     1621 cgccgccgat ggaataataa gttttgctgt agacgacttc atcgccgtta taggcgtgga
     1681 tttgcatacc gttttcatgc agcggcaggt tgccgttatg aaaacgcatc ccgttgtcgc
     1741 gcgggaaatc cacttcatgc cgtccctgtg ccagcagcag acgttcgcgc tcttctacgt
     1801 cgcgaataaa accgggaata ctgtcgatat ccacggtggc aggttcgtta cctgcaagac
     1861 ccataataat ggcgatatcg gtgtggtggc ctttacccgt cagcgacagt gaaccataaa
     1921 cgtccacggc aacgcgagta acgctatcca gtaagccttt ttcgaccaga tcatcgacga
     1981 actgtttacc cgccttcata ggccctacgg tatgggaaga tgagggacca atccccacct
     2041 taaacatgtc gaatagacta atcacgataa tactcctgac aaggtgactg gacttccagt
     2101 aacgaactaa taacagcgca tagtgtaaga gggaacgcgg cgactggctt aactattcac
     2161 atgaattaaa ctaatcaaaa accgcgattt aactaatatt gcgatgattg tctcaagtag
     2221 tgttttgtag atgtaaagat aagtatagtc agggtttcac accaatttgc agcgccagct
     2281 cacgaattat gcctgcggtc attccccata caaaatactg ttcgtaccag gacagccata
     2341 cccgatgtga atcgccacgg cggtagatat ctaaagggtg ataacgaccc agatgtaatg
     2401 cctgggcgag cggcatttca aacaccgccg agacttcatc ttcactggcg cgatacggca
     2461 gatcgggcgg gataatgccg atcactgggg ttacctgata gccagttacg ctatcgacgg
     2521 gcggcagcac gccgataact tcaacggcgg aaggcggtat agcgacctct tcttcagctt
     2581 cgcgcagcgc ggcggcgata actgatgcgt ccgtgtcatc gactgcacct ccagggaatg
     2641 ccacttgtcc agcgtgttta cgcagatgaa ttgaacgctg agtcagcaac aaccccggtt
     2701 gcggtcgacg gacgatgggg attaacacgg cagcctgacg atgatttagg gtttcccggt
     2761 taatttgcgg gcgcaaaagt tgaaagcgag ataaaaaatc atcaagcgtc aggctacggt
     2821 attccacgtc ttacttctcc agttggcgta atatcttagt aactttatca aaagtttcct
     2881 gatattccgc ttcttcctgg ctatcggcga caattccacc gcccgcagag cagtatattt
     2941 gtccgttaat ggcagtcagc gtgcggatag taatgctggt atccatgttg ccgcaaaagc
     3001 tcaaatagcc aatgctgccg caccaggcat tacgtcgctg cggttccagt tcgtcgataa
     3061 tttccatagc ccgtactttc ggagccccgg ttattgagcc accaggaaaa gctgcgcgca
     3121 gcagatcgct ggcgtgtaac tgttctggta gtcgcgccgt tatagtgctg accagatgat
     3181 gcacggcagg gaagggttcc accacgaaga gctctggtac ttttaccgaa ccggctacgg
     3241 caacacgacc gatatcatta cgcattaaat cgacaatcat cagattttcg gcacgatctt
     3301 tcgctgagtt cgccagtttt tctgcttgtt tgctatcttc ctgaggatcg ggcaggcgtg
     3361 gtagcgtgcc tttaatcggg cgggtctgga tttcactatt atcacaaaga ataaaccgct
     3421 ctggcgaaag gcttaaaatt gcaccctgtt caagacgtaa aaaagcgcta aatggcgcgc
     3481 ggttggcctg attaagctga aggaatgcct gccattcatc gccagaatag gtcgcatgaa
     3541 aacgctgggc gagattcacc tgatagcaat caccgctgtg cagatattcc tgtacctggc
     3601 gaaatttttc gccgtactgc tcgcgggtca tattggattg ccagtcggaa gtgagcgtga
     3661 aatcttcctg cggcgagaat tgctggcttt ccagccaggc ccgacgggca ttgacatcat
     3721 tatgactcag caaagaaact gtatgacgct ggtggtcgac aatgagcgcc caatcgtaga
     3781 tacccactgc catatccggc agaacgatat cttgttccgc aatttctggc agtgactcaa
     3841 aacggcggcc cagatcgtag ccaaacaacc ccagtgcgcc gccctgaaat ggcaaatctt
     3901 cgttatgcgt tgggcgaatg tctgcgcgat ccagcacctg ctggagcacc tgtagcgggt
     3961 catcagtggt cgttgtgcgt ttttcgcttt cactaacaac ggtttcttta ccgaaagtgg
     4021 ttaaagtgca aatcggctcg gcgaccacaa tatcaaagcg gctatacgga tgatcggcat
     4081 agccggagtg taaaagcatc gcccacggca ggtggcttaa gcgggagaaa taaaattcag
     4141 cggcgtcctg acgccagggt aaagtaatca cagcgggaga taacgtcttc atcagtcctg
     4201 actctactgg ctatgtgccg ggtagcatga aataattagc gccaacaatt tagcaggagt
     4261 taacaatgtt tgcaggttta ccttcactca cccatgaaca gcagcaaaaa gctgtcgagc
     4321 ggatccagga actgatggca caagggatga gcagcggcca ggcaatcgcg ctggtggcag
     4381 aagagctgcg tgccaaccac tccggcgagc gaatcgtggc gcgttttgag gatgaagacg
     4441 agtaacacac taatccgtat cgatttc
//
LOCUS       DAJVTY010000222         4459 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVTY010000222 DAJVTY010000000
VERSION     DAJVTY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.223"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            142..2139
                     /gene="betT"
                     /locus_tag="OQQ66_003847"
     CDS             142..2139
                     /gene="betT"
                     /locus_tag="OQQ66_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCS1906589.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2202..3479
                     /locus_tag="OQQ66_003848"
     CDS             2202..3479
                     /locus_tag="OQQ66_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCS1906590.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKK"
     gene            3725..3886
                     /locus_tag="OQQ66_003849"
     CDS             3725..3886
                     /locus_tag="OQQ66_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906591.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVSTTDEK"
     gene            complement(3930..4459)
                     /gene="istB"
                     /locus_tag="OQQ66_003850"
                     /pseudo
     CDS             complement(3930..4459)
                     /gene="istB"
                     /locus_tag="OQQ66_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1072 a         1032 c         1123 g         1232 t
ORIGIN      
        1 tggtagtgtc cgctattgcc agtacacctc aacattccac catgcattcc gattaacgcc
       61 gcatagccag ttgaactttg ctactttgtg agaggtagta ccttctatcc agtgcgaatt
      121 taattaatgg aataaatgat tatgagtgaa aatgatacaa tcccaaagaa gtctacaagt
      181 cagattaaca aagcggtatt ctttacatct gctttgctaa ttttccttct tgtcgccttt
      241 gccgccgtat tcccggatgt cgccgacaaa aattttaaac tacttcagca acaaatcttc
      301 acgaatgcca gctggttcta catccttgct gtggccctga ttttactgag tgtcacgttc
      361 cttggactct cacgctacgg tgatatcaag ctgggcccgg accatgcgca gcctgatttc
      421 agctaccact cctggtttgc gatgcttttt tcggcaggga tggggatcgg cctgatgttc
      481 tttggcgttg ccgaacctgt aatgcattat ctttcgccac ccgttggcac tccagaaacc
      541 gttgcggcag ctaaggaagc aatgcgtctg acctttttcc actggggact gcacgcatgg
      601 gcaatttatg ccattgtggc gctgattctg gcgttcttca gttaccgtca cggtctgcct
      661 ttaactctgc gctccgcact ctatcccatt attggcgatc gcatatacgg acctgtagga
      721 catgcggttg atattttcgc tgttataggc acggtctttg gcgttgcgac atcactgggt
      781 tacggtgttt tgcaggtgaa tgccggtttg aaccatcttt tcggggtgcc catcaatgaa
      841 acggtgcagg taattctgat cgtggtcatc acggggttag cgacgatttc agtggtgtcc
      901 ggtctggata agggaatacg tatcctgtct gaactcaatc tgggtctggc tttgttgctc
      961 ctggcgctgg tcctgtgtct gggaccaacc gtgcttctgc tgaagtcatt tgtggaaaat
     1021 acgggcggtt atctttcgga actggtgagt aaaacgttca acctttacgc gtatgagccc
     1081 aagtcgagca actggctggg gggctggaca ttactgtact ggggatggtg gctttcatgg
     1141 tcgccgtttg tggggatgtt catcgcacgg gtctcccgcg ggcgaaccat tcgcgagttt
     1201 gtcaccggcg tgctgtttgt tcccgcgggt tttacgctaa tgtggatgac ggtgtttggt
     1261 aacagcgcga tctatctcat tatgaaccag ggggccacag acctcgccaa tactgttcag
     1321 caggatgtgg cgctggccct gtttaatttc ctggagcatt tcccgttctc ttctgtgctg
     1381 tcattcattg caatggcgat ggtcatcgtc ttctttgtaa cgtctgctga ttcgggggca
     1441 atggttgtgg atactctggc atcaggtgga gtggcaaaca cacccgtctg gcagcgaata
     1501 ttctgggcct cgctcatggg cattgttgca attgcgcttc tccttgccgg agggctaagt
     1561 gcgctgcaaa cggtgacaat agcgagtgca ttgcccttct cagtgatctt actaatatct
     1621 atatacggac ttttaaaagc tttgcgccgg gatttgacca agcgtgaaag cctgagcatg
     1681 gcgacaattg ctcctacggc tgcacgtaac ccaattcctt ggcagagaag gttacgcaat
     1741 atcgcgtatc tgccgaagcg atctcttgtg aaacgtttta tggacgacgt tatccagccc
     1801 gccatgacgc tggttcagga ggaactgaac aagcagggga cgataagcca cattagtgat
     1861 gcagtcgacg atcgtattcg tcttgaagtc gatttgggca acgagctgaa ttttatatat
     1921 gaagtgaggc ttcgcgggta tatctcaccg accttcgcgc tcgccgcaat ggataatgat
     1981 gagcagcaga ctgaacaaca tcgatattat cgcgctgagg tttatctcaa agaaggcggt
     2041 caaaattatg atgtgatggg ctggaaccag gaacagctga ttaatgacat actggaccag
     2101 tacgaaaaac acctgcactt cctgcacctg gttcgttaat agcaacatgc cgtcctgggg
     2161 gcggcaatta ttatcccggc cgcaatatga gggaatgcag aatgatttca cgctggaaat
     2221 ggatgctgaa gcagacaatt aaaaaactat ggttcagggc aacgttattc gcaattgtcg
     2281 cgataataac ggccctttta tcaattcttt ttaaatcaat gatacctgag tcggtttccg
     2341 tgaaggttgg tgcggaagca gtcgataaca ttctgaacat actggcatcg agtatgctgg
     2401 cagtgaccac attttcgctg agtatcatgg tcacagccta cggttcagcc actactaatg
     2461 tgactcccag agctacgcgt ttagttgttg aagacgtcac cacacaaaat gtactggcca
     2521 ccttcatcgg ttcttttctc ttcagtctgg tagggattat tgccctcaat atgggagctt
     2581 atggagaaag ggggagagtc attttattca ttgtcacact ggttgtcatt gccttaatcc
     2641 tcatcacatt gcttcgctgg atacagcatt tgacctctct ggggagggtt ggtgagacaa
     2701 cggcacaagt agaacaggcg gccatcgaaa catttattgc gagagcaaga aatccctgtc
     2761 tcggcggata tccatggctt gagaacaatg aacagccgaa aggaacggtt gcagtttatc
     2821 cgaagaagat tggctatgtt gaatatattg atatggtgaa acttagcaag ctgctgacca
     2881 atgatccccg tcatgtatac ctcgtggcgc agccaggcag tttcatacat ccgtccatgc
     2941 cagttttgta cctgagtcag ggccaggagt cgtcaatcaa caccgattta cttgagacga
     3001 ttattgtctc ggatgcacgt tcatttgctc aggatcctcg attttgtctt agcgtcatgg
     3061 ccgaaatagc ctgccgggcc ctttcccctg cagtgaacga tcctggaacc gccattgatg
     3121 tcattggcag aggtgttcgt atactttcca cttacgcgca gaataaatct gatgaaatag
     3181 aagtgaaata tccttcagta catgttgcac cacttcagaa taacgatcta ctggaagact
     3241 ttttctcacc tgtcgcgcgc gatggtgcca gtatgaggga gattcagata agagtcctta
     3301 aaggactgtc gatgctgagt aaaggttggc ctgagatatt tgctgaagcc gcacataccc
     3361 tggcatttga aacattagag catgcaactc gtgctgacca tatagattct gatagatatc
     3421 taataaaatc aacttattat aatttattta gtggcgaata ttctaataaa aaaaaatagc
     3481 tgcagagcaa gcaattcggt tgttggcaag tcaagggcaa agcattcgcg tgagaatggg
     3541 cgtatgcttt gtgccaaaag caaacgttgc taattctgca ctgttcagcg atggcgcagt
     3601 agcgacaggc actgccactg tccagtcggc aaaagcgaac actttgccac tgcggggata
     3661 aactgattga agctctcgcg caaaatcact tattcttttg acaataaaat gtaggagatg
     3721 ggtcatgaca ggcgaaaatc gtggccgggg aaggccgcga aagacagaaa gtacctatgc
     3781 agatactcgt aatgatctga tccgcagtgg ccttgaactg ctgacgcaga atggttttct
     3841 tgcgacggga gtcgatgcca ttgtgtcaac gacggatgaa aagtgatcca cttatatctc
     3901 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttcagc tataaccccg
     3961 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgac atgtgagtgg
     4021 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
     4081 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
     4141 atgacctaga agaacagttt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
     4201 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
     4261 cgttgtgccg tagataactg aagtaacaga tctgctgctg ttgtgaagcg aaccttgata
     4321 cctgcacgga ctgcttcata gcccatcgct attgccagat gggttttccc cacacctgat
     4381 ggccccagta atacgatatt ttcattacgt tctatgaagc tgagtgagcg taacgactgg
     4441 agttgcttct gcggtgctc
//
LOCUS       DAJVTY010000223         4397 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVTY010000223 DAJVTY010000000
VERSION     DAJVTY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.224"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            221..593
                     /locus_tag="OQQ66_003851"
                     /pseudo
     CDS             221..593
                     /locus_tag="OQQ66_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1155..2102)
                     /gene="icsP"
                     /locus_tag="OQQ66_003852"
     CDS             complement(1155..2102)
                     /gene="icsP"
                     /locus_tag="OQQ66_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1906592.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3483..3635)
                     /locus_tag="OQQ66_003853"
                     /pseudo
     CDS             complement(3483..3635)
                     /locus_tag="OQQ66_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3691..4383
                     /gene="ospZ"
                     /locus_tag="OQQ66_003854"
     CDS             3691..4383
                     /gene="ospZ"
                     /locus_tag="OQQ66_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCS1906593.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1350 a          862 c          849 g         1336 t
ORIGIN      
        1 tggttcaaca atatgcagat ggtacacaac ctctttaacc agcccatccg gattattcag
       61 agcccttccg caatccggaa taaaagtgct gttttaccgt gaaccctctg aaaatcagcg
      121 agcagtgttt taaccagccg acgttcagag cgtactgaca gacggtggac aatttgaata
      181 aacagttcaa tcagaccgta gatgctctcc cgttggcgac atgctgggag cggtggaaca
      241 ccgcttcggc aacgagcttc cggcgtctcc agtgaagtga ctgacggata atggttcatg
      301 ctaccgggct aatgaaacac gccagttcac ccggatgttg ggactttgtt gaaccgaaga
      361 acacggcggt gcggagtccg gagagtaaca gaatagcaaa gagcttcgtg aaaacaataa
      421 agtgtgacta catcagtatc atgcccaaac ctgacgggtt aacggcagca aagaaccttg
      481 cagaggcatt cgagcattat aacgaatggc atccgcatag tgcgctggat tatcgctcgc
      541 cacgggaata tctgcggcag cgggccaatg ataacaggtg tctggaaata taggggcaaa
      601 gccacccacg aacgatccag catataatca agaaattaga tttcatttaa cataagatag
      661 attatgtgca ccaagaggca tttccttaat ttctgaattt acaggctctt ttgctctctt
      721 tgctatgctg tggatttgga aagatagagt gctggctgat tatttaggta ttttcatcag
      781 catgtccctg atagcactgt tccatcagcg ctttcaggcg ataatagtag accttgggta
      841 gcctctgctc atgtgtaact tgataccccg agaacagcca cgatgctcta agcggttctt
      901 atttctccgg tctcagggtc ggtaatgcca acagtaagtc cacagtaatc aacgaagagt
      961 tttttgaacc gccccggaaa tcctggagac taaacgccct gagaaagagg taaacagaat
     1021 gactaaaaat acacgttttt cccccgaagt ccgtcaacgg gcagttcgta tggttctgga
     1081 aagtcagggc gaatatgact cacaatgggc ggcaattttt agccatgaaa acgtaatcaa
     1141 ctcggtgaac caattcaaaa aatatacttt atacctgcgg aagtgagaaa accaatatat
     1201 tcaatacttg cactattttt aatggtgcca gatatattgg tactttgctc ataaagagat
     1261 gtatcacctt ttttattaga aatgtaattt ctggagcctt ctatataaaa ttttgcactg
     1321 ggggtgacat agtatccaat attcgcagca acagagcagt aattttggtt ttttatttca
     1381 tctttaaaaa ttgtctcagt ctgatagtgt ttatctgtat cagatgaacg aacccaacca
     1441 ctatatttca gttctgctcc aaactcaaaa ttctcatggc ggtaattagc agttaatcca
     1501 atataaggta ttttgaagag ctgtttgtaa cctattgttc tttcaccact aggatgtgcc
     1561 ccttttttat tcctgttccc cccgttttca ctataaatat aactccctcc cattgcatta
     1621 aaactgtaac gactctcttg gtaacctgct attagtccaa gtcgataatc caaattattt
     1681 aataaccaac ctttaagatt cagctcgaat tcgttagcat cacggagatg cgtattggga
     1741 tgccagctct ggtcggtcca tacctgaggg gtattggaat tgttccagtc tttatcaacc
     1801 atgcttgctt tctggttacc taaagtagtc caaccggaaa ctccgaaaga cacttttgga
     1861 attagcttcc aatcgatgcc acctctaaca atagtcgcat tactgtattt ccagtccagt
     1921 tgactaattt ttctcccccc ttccttggga tgataaacgc gttcttttgt tttgccactc
     1981 agagatccta gactaatatc cgtactgatg ttatccggta tgaaaagtgg atagttagtg
     2041 gtagcatgtg tagtaaagat cgcaggtaca caaagtgcaa gtacaaggaa ttttaatttc
     2101 atcgagaact ctactttttt ggttgaaatg tccatgatct tcttacttta taagataaac
     2161 gtcaatgcca agcattattt taattaaata tttgtttatg ttaccaagaa catatctttt
     2221 attataatat ttattgaact tttgtaaaaa taaaaaaata cctcattgtg cgaataaagt
     2281 aacgggggcc atagatttct ctacctacct tttggtgttc atacctgagg ctgatagttt
     2341 caggattccg cgacaattct actcaccttc ttgttctctc gccaatatga aatcaatcag
     2401 aaaagcgttc cataagaaca acgataagac aataaattgt catcacggct gcaatagcca
     2461 acgtgccttt cagtaccaac atgacaacct cctgtatttg acgtaacgtg tgctgataaa
     2521 tatgtatttg tatctcaact gactcaatac aacacttaag tatagttttt cagattttgt
     2581 ttttcccgtg aaatattatg gaaatggcag aagcaataca ttgaattccg attcgagtga
     2641 atacggctag cgggcacctc actttagcac tgaagccatc aacttttatt tgacttattt
     2701 caatggaaag ctttctcact atcagtacag gtaattccca tccttttaaa gtcgagagtc
     2761 agactgcgac taatacagtt aaagtgacca aactcagtta tcctctacac attttacctc
     2821 atgtttactt ggacaattca ttggtgcttt tataaaagaa taaatattct actaatttaa
     2881 tgcatcacct aaaatagtat tttttataat agaactgtat ctatagtata ttattaaagc
     2941 ttaattattt attatgcttt aatgtggttg tccgattaag gactaatcct tgaaaacgcc
     3001 gtatagggcg ggagttcact ctattgcgat aacgtaaccg tacaatattg tgcgtttccc
     3061 aaattgacca ctactgaact attctgggat ttaaatgcaa taaaatggtt ggttgaaggt
     3121 cgtggtgatg atgttagtcc ttttgagaat attccagtgc cagatcttac ttataataaa
     3181 gaggcttggc agtttggata agctattgtc cttattccac taatacaaac tttttgtaaa
     3241 gtatattaaa tatacttcat ttcatactga aatccccgga agtattcaat caaacaatta
     3301 caccataaaa ggaagaggga gccccaatta agttatcaag catgtttatg atacctgcag
     3361 ggtataaatt ttctggctgc aagcagacac atataaaacg tctttattga tcctacccac
     3421 ataatatgga cgcagtcgta agtgaggctc tgtgagccta cccacgtaat atggacacag
     3481 gcctaagcga ggttctggtt ttcaaattgt tccggactga ggccgccaca ccaacggtgc
     3541 cgtcgccagc gattgtaatc gcactcgata taattaaaca ctgttgcccg cactatttcc
     3601 cgatagataa agtgttctcc gtggagacat tccacttagg aacaattata ttgttcatgg
     3661 taatgtttat atttgagtat agagattaaa atgattagtc ccatcaagaa tattaagaac
     3721 gtatttccaa taaatactgc aaatactgaa tatatagtga gaaatattta tcctagagtt
     3781 gaacatggtt actttaatga atcaccaaac atatatgaca agaagtatat ctctggtata
     3841 accagaagta tggcacaatt aaaaatagag gagtttatta atgagaaatc cagacgactt
     3901 aattatatga agacaatgta ttctccatgt ccggaagatt ttcagcctat ttccagagac
     3961 gaagccagta taccggaagg aagctggttg acagttatat ctggaaaacg tcctatgggg
     4021 caattttctg tggatagttt gtaccatcct gacttacatg cattatgtga gctcccagag
     4081 attagttgta agatcttccc taaagaaaat agtgattttt tgtatataat tgttgtattc
     4141 agaaatgatt ctccccaagg agaactacgg gcaaatcggt ttatagaact atatgatata
     4201 aaaagagaga ttatgcaagt gttaagagat gagtcgccag agttaaagtc tattaaatct
     4261 gagattatta ttgctcgtga aatgggagaa ctttttagtt atgcatcaga ggaaatagat
     4321 agctacataa aacaaatgaa tgacaggttt tctcaaatca aggctcgaat gtctgttact
     4381 taatgtatat taattgt
//
LOCUS       DAJVTY010000224         4375 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVTY010000224 DAJVTY010000000
VERSION     DAJVTY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.225"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(33..1727)
                     /locus_tag="OQQ66_003855"
     CDS             complement(33..1727)
                     /locus_tag="OQQ66_003855"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HCS1906594.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            complement(1779..2201)
                     /gene="merC"
                     /locus_tag="OQQ66_003856"
     CDS             complement(1779..2201)
                     /gene="merC"
                     /locus_tag="OQQ66_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HCS1906595.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            complement(2237..2512)
                     /gene="merP"
                     /locus_tag="OQQ66_003857"
     CDS             complement(2237..2512)
                     /gene="merP"
                     /locus_tag="OQQ66_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HCS1906596.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            complement(2526..2876)
                     /locus_tag="OQQ66_003858"
     CDS             complement(2526..2876)
                     /locus_tag="OQQ66_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HCS1906597.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            2948..3382
                     /gene="merR"
                     /locus_tag="OQQ66_003859"
     CDS             2948..3382
                     /gene="merR"
                     /locus_tag="OQQ66_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HCS1906598.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
BASE COUNT          805 a         1353 c         1396 g          821 t
ORIGIN      
        1 cgtgtaggcg ctcatcgcac acctccttgt cctcacccgg cgcagcagga aagctgcttc
       61 acatccttgt tgaaggtctg cgccgcgagc ttcaaccctt cgaccatcgt caggtagggg
      121 aacaactggt cggccagttc ctgcaccgtc atccggttgc gaatcgccag tgcggccgtc
      181 tggatcagtt cgcccgcttc cggggccact gcctgcacgc cgatcagtcg tccgctgcct
      241 tcttcaacca ccagtttgat gaagccgcgc gtgtcgaagt tggcgagcgc gcgcggcacg
      301 ttgtccagcg ttagcgtgcg actatcagtt ttgatgccgt catggtgcgc ttccgcctcg
      361 ctgtagccta cggtcgccac ttgcgggtcg gtgaacacca cggccggcat cgcggtcagg
      421 ttcagggccg cgtcaccgcc ggtcatgttg atcgcggcgc gagtgccggc cgctgccgcc
      481 acatagacga actgcggctg gtcggtgcag tcgcctgcgg cgtagatgtg ttccacgctt
      541 gtacgcatgc cggggtcgat gacgatagcg ccttgcgggg tgagcgtgac gcccgtcgca
      601 tccagtgcca gcttgcgtgt gttgggcgcg cggccggtgg cgaccagcag cttgtcggcg
      661 cgcagttcgc cgtgcgccgt ggtgagcacg aattcgccgt ccccttcacc attgatatac
      721 gcgacctggc tggcctgggt gtgttccctc acctcgatgc cctccatgcg gaatgcggcc
      781 gtgacggctt cgcctatagc tgggtcttcg cggaagaaca gcgtgctgcg agccaggatc
      841 gtcaccttcg ctccgagtcg ggcgaacgcc tgcgccagct ccagcgccac cactgatgag
      901 ccaatcacgg ccaggcgctt aggaatcgtc tcgctgacca gcgcttcagt ggaagtccag
      961 tacggagtgt ctttcaggcc gggaatcggc ggcacggccg ggctcgcgcc ggtggcgatc
     1021 aggcagcggt cgaatgccac cacgcgctcg ccgccgtcgt tgagttgcac gatcaggttg
     1081 cgattgtcct taaagcgggc ggagccgtgc agcacagtga tcgccggatt gccctccaag
     1141 atgccttcgt acttggcgtg gcgcagttca tcgacgcggg cctgctgctg ggccagcagc
     1201 gccgtgcgct ggatggtcgg cgtggtagcg gcgatgccgc catcgaacgg gctttcccgg
     1261 cgcagatggg cgatatgggc ggcgcggatc atgatcttgg acggcacaca accgacattg
     1321 acgcaggtgc cgccgatggt gccgcgctcg atcagcgtga cacgtgcgcc ttgctcgacg
     1381 gccttcagcg ccgctgccat cgcggccccg ccgctgccga tgacggcgat atgcaatgcg
     1441 ccgctgctac ccgtcttgtc gtttctgccc agcagatcgc gcatcttgtc gagcaatccg
     1501 cccggcgtcg aaactgaggg ggcatcggcc agcgtggccc gataaccgag tccagctaca
     1561 gcggccgtca gcgcgtcggg tgacgtgccg acctcaatgg cgagcttggc gctgcccttg
     1621 gcgtaggaga catccgctga ttgcacgccg ggcactttct ccagggcgtc cttgacatgc
     1681 actgcgcacg agtcgcaagt catgccggtg attttgagag tgctcatacc atcgttcctt
     1741 attcgtgtgg gccgccgtgt cgcacggtca gccgtctttc acaagcgctt ggcggggagt
     1801 tcgcagccgt ccggtccgca acggcgatgc gccggcgaca cgaagtccca gatcgacacc
     1861 ccaatcatca aggccaggcc gacgtacatc aggttcgccg tccaccagtt gccgagcagc
     1921 cagaccgtgg ccgcaaacac gatggccggg ccgatcatgc cgagcagact gcgcagccat
     1981 tgccgatgac tgaaccaacc cagcgcgttc gccaggaagg ccagcgcggc aaacagcggc
     2041 agcaggcggc tgatgaacag tccctcgtac tggctcaaga agcccagccc gatggccgcg
     2101 ccgaagctgg cgagggctgg aaagcaggcg gcgcagccca tcgcggaaac gacgctgccg
     2161 agcgcgccgg ttttatcggc aatgcgtgtc atcagtccca tgaagcggct ctcgctgttg
     2221 tcgttggctt gctggctcac tgcttgacgc tggacggata gccggcgtct gcggtggcct
     2281 tggtcagctt ctgtacgctg gccttggtgt cgtcaaaagt gacgacggcc tcgcgcttct
     2341 cgaagcccac atcgaccttg ctcacgcctt cgaccttgga gagcgctttc ttgactgtga
     2401 tcgggcaggc ggcgcaagtc atgccgggaa ccgctagcgt gacggtctgg gtagcggccc
     2461 acaccggggc aacagcggcg gcgagggcaa gggaggcaaa cagtttcttc atgatgaact
     2521 cctggttaat agaaaaatgg aacgacatag ggaaatccaa gcgcgaccag gaccagcacg
     2581 gccacgatcc agaaaatcag cttgtaggtg gcgcgcacct gcggaatcgc gcagacctca
     2641 cctggcttgc atgcctgcac gggccggtaa atccgcttcc aggcgaagaa cagcgccact
     2701 agcgccgcgc cgatgaacaa cggtcgatag ggttccagca ccgtcaggtt gccgatccaa
     2761 gcaccggaga agcccagggc gaccagtact agcggcccca ggcagcaggt cgatgcaaga
     2821 atggcggcca gcccgccggc gaagagcgca ccgcgcccgt tttgtggttc agacatacgt
     2881 tggccctttt gaatttggat tggatagcgt aaccttactt ccgtactcat gtacggagtc
     2941 aagcgatatg gaaaataatt tggaaaacct gaccattggc gtttttgcca aggcggccgg
     3001 ggtcaacgtg gagacaatcc gcttctatca gcgcaagggc ctgttgcggg aaccggacaa
     3061 gccttacggc agcatccgcc gctatgggga ggcggacgtg gttcgggtga aattcgtgaa
     3121 atcggcacag cggctggggt tcagtctgga cgagattgcc gagctgttgc ggctcgacga
     3181 tggcacccac tgcgaggagg ccagcagcct ggccgaacac aagctcaagg acgtgcgcga
     3241 gaagatggcc gacttggcgc gcatggaaac cgtgctgtct gaactcgtgt gcgcctgcca
     3301 tgcacgaaag gggaatgttt cctgcccgtt gatcgcgtca ctacagggcg aagcaggcct
     3361 ggcaaggtca gctatgcctt agcgtgcttt attttccgtt ttctgaggtg ccccctaatt
     3421 gagcatttcc aggcgcttgc gcctggtcaa cgaaagagtc agcgccgtag gcgctgccat
     3481 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc
     3541 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg
     3601 cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt
     3661 aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc
     3721 tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc
     3781 cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat
     3841 accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc
     3901 aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct
     3961 gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc
     4021 ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt
     4081 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
     4141 gcccagcggc gagggcaacc agcccggtga gcgtcggaaa ggcgctggaa gccccgtagc
     4201 gacgcggaga ggggcgagac aagccaaggg cgcaggctcg atgcgcagca cgacatagcc
     4261 ggttctcgca aggacgagaa tttccctgcg gtgcccctca agtgtcaatg aaagtttcca
     4321 acgcgagcca ttcgcgagag ccttgagtcc acgctagatc tatctcatct gcgca
//
LOCUS       DAJVTY010000225         4371 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVTY010000225 DAJVTY010000000
VERSION     DAJVTY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.226"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            124..453
                     /locus_tag="OQQ66_003860"
     CDS             124..453
                     /locus_tag="OQQ66_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1906599.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            701..946
                     /gene="nrdH"
                     /locus_tag="OQQ66_003861"
     CDS             701..946
                     /gene="nrdH"
                     /locus_tag="OQQ66_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1906600.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            943..1353
                     /gene="nrdI"
                     /locus_tag="OQQ66_003862"
     CDS             943..1353
                     /gene="nrdI"
                     /locus_tag="OQQ66_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1906601.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGMPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1326..3470
                     /gene="nrdE"
                     /locus_tag="OQQ66_003863"
     CDS             1326..3470
                     /gene="nrdE"
                     /locus_tag="OQQ66_003863"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1906602.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3480..>4371
                     /gene="nrdF"
                     /locus_tag="OQQ66_003864"
     CDS             3480..>4371
                     /gene="nrdF"
                     /locus_tag="OQQ66_003864"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1906603.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFF"
BASE COUNT         1079 a         1115 c         1116 g         1061 t
ORIGIN      
        1 gcctacctta cctgattgcg cattcacaac gctttcagaa aagtccataa agccgcgatg
       61 aacctcagtt aagaaaatta tggtctacac tgaaaattac atcgaattct aatgggggat
      121 catatgttta accgaccgaa ccgcaacgac gttgatgatg gcgtgcagga tatccagaat
      181 gatgtcaatc aattagctga cagcctcgaa tctgtattga agtcctgggg cagcgacgcc
      241 aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt tgctgaaaga aaccagggcg
      301 cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg atgccgttgg ctgcgcggat
      361 tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag cagctgcggt aggtattttt
      421 atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa atgataataa atacgcgtct
      481 ttgaccccga agcctgtctt cggggtttct ttttgcctgg tgaatcacaa aaatccccct
      541 cccccgtcac gctcatatcc agggtaattt cgatcactat ttgctatata ttgtgtagtt
      601 gaatcttttt tcaactacat ctagtatctc tgtatcaaca gagagacaac ccgacgcgta
      661 tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc atgcgcataa ctatttacac
      721 tcgtaacgat tgcgttcagt gccacgccac caaacgggcg atggaaaacc gggggtttga
      781 ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg gaagcgttgc gtgctcaggg
      841 ctttcgtcag ttgccggtag tgattgctgg cgatcttagc tggtctggtt tccgtccgga
      901 catgattaac cgtctgcatc cagcgccaca cgcggccagt gcatgagcca gctcgtctac
      961 ttctccagca gctccgaaaa cacgcagcgt tttatcgaac gtttaggtat gcccgcggtg
     1021 cgcatcccgc tcaatgagcg ggaacggatt caggtagacg agccttacat cctgatcgtg
     1081 ccctcttacg gcggcggcgg tacggctggc gcggtgccac gacaggtaat tcgcttttta
     1141 aacgacgagc acaaccgggc gttgcttcgc ggcgttattg cttctggtaa tcgcaacttt
     1201 ggtgaggcgt atggccgcgc cggagatgtg attgcccgga aatgcggcgt gccgtggctg
     1261 taccgttttg aactcatggg tacgcaaagc gatatcgaaa acgttcgtaa aggagtaacc
     1321 gaattttggc aacgacaacc gcagaatgcc tgacgcagga aacgatggat taccacgcgc
     1381 tgaatgcgat gcttaacctc tacgatagcg caggtcgcat tcagttcgat aaagaccgcc
     1441 aggccgttga cgcctttatg gcgacgcatg tgcgtccgaa cagtgtgacc ttcagtagcc
     1501 agcagcagcg cctgaactgg ctggtcaacg aaggttacta tgatgaaagc gttcttaatc
     1561 gctactctcg cgactttgtc attacgctgt ttgcctacgc acacaccagc ggttttcgtt
     1621 tccagacatt cctcggggca tggaagtttt acaccagcta tacgttgaag acattcgacg
     1681 gtaaacgtta tctggaagat tttgccgatc gagtcacgat ggtggcgctg acgctggcac
     1741 aaggcgatgc gacgctggcg ttgcaactga ccgatgaaat gctgtcagga cgctttcagc
     1801 cagccactcc gacattcctc aactgcggta agcagcagcg cggcgaactg gtttcctgtt
     1861 ttctgctgcg tattgaagac aatatggagt cgattggccg ggcggtaaat tccgcactgc
     1921 aactgtcgaa acgcggcggc ggcgtggcat ttttgctgtc gaatctgcga gaagcgggcg
     1981 cgccaattaa acgtattgaa aatcaatctt ctggcgtaat tccggtgatg aaaatgctgg
     2041 aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca gggggcaggt gcagtctatt
     2101 tacatgctca tcatcccgat attctgcgtt ttctcgatac aaaacgggaa aatgccgacg
     2161 aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat cccggatatc actttccatc
     2221 tggcaaaaga gaatgcgcag atggcactgt tttcgcccta tgacgttgag cgagtttatg
     2281 gcaagccgtt tgccgatatc gccatcagcg aacactatga cgaactggtt gccgatgaac
     2341 gcatccgcaa aaaatacctc aacgcccgtg atttcttcca gcggctggca gaaatccagt
     2401 ttgagtccgg ctatccctac atcatgtatg aagacacggt aaaccgtgct aaccctgtcg
     2461 ccgggcgcat aaatatgagt aatctctgct cagagatttt gcaggtcaac tgtgcctcag
     2521 agtatgacga gaatctcgac tatgcccgca caggccatga tatctcctgc aatttaggtt
     2581 cgttgaatat tgcgcacacc atggattccc ccgattttgc ccgcacggta gagactgccg
     2641 tgcgcggttt aacggcggtg tcagatatga gtcatatccg cagcgtgccg tccatcgaag
     2701 ccggaaatgc cgcctcacac gccatcggac tggggcagat gaatttacac ggctatctgg
     2761 cgcgagaagg catcgcttat ggttcgccgg aagcactgga tttcaccaat ctctatttct
     2821 atgccatcac ctggcacgca ctgcgtacct cgatgttgct ggcgcgcgaa cgcggtgaaa
     2881 ccttcgccgg gttcaaacag tcacgttatg ccagtggtga atattttagc caatatctgc
     2941 aaggtaactg gcagccgaaa acggcgaaag ttggcgaact gtttgcccgc agcggtatta
     3001 cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga cgtgatgcgc tacggcattt
     3061 ataaccagaa tcttcaggcg gtgccgccaa ccggttctat ctcttatatc aaccatgcta
     3121 cgtcgagtat tcatccgatt gtggcgaaag tagagatacg caaagagggc aaaacaggac
     3181 gcgtttacta ccctgccccg tttatgacta acgagaatct ggcgctgtat caggatgctt
     3241 acgaaatcgg cgcagaaaag atcatcgaca cctacgccga agcgactcgc catgtcgatc
     3301 aggggctgtc gctgacgctg tttttccccg ataccgccac cactcgcgat atcaacaaag
     3361 cgcagattta cgcctggcgc aagggtatca aaacgctcta ttacattcgc ctgcgtcaga
     3421 tggcgctgga aggcactgaa attgaaggct gcgtctcctg tgcactttaa ggaatatcta
     3481 tgaaactctc acgtatcagc gccatcaact ggaacaagat atctgacgat aaagatctgg
     3541 aggtgtggaa tcgcctgacc agcaatttct ggctaccaga aaaggtgccg ctgtcgaacg
     3601 atattcctgc ctggcagaca ttaactgtcg tagaacaaca actgacgatg cgcgttttta
     3661 ctggcctgac gctgctcgac acgctgcaaa atgttatcgg cgcgccttcg ctgatgccct
     3721 atgcactcac gcctcatgaa gaagcggtat tatcgaatat cagctttatg gaagcggttc
     3781 atgcccgctc ttacagttcg attttctcga cgctgtgtca gaccaaagat gtcgatgccg
     3841 cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa agctcagatt attcagcaac
     3901 attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag tgtgtttctt gaatcttttt
     3961 tgttctattc cggtttctgg ctgccgatgt atttttccag ccgcggaaag ctgaccaata
     4021 ccgcagatct gatccgtctg attatccgcg atgaagcagt ccacggttac tacataggct
     4081 ataaatatca gaaaaacatg gaaaagatat ctctgggaca acgtgaagag ttgaagagtt
     4141 tcgccttcga tttgttgctg gaactctacg acaacgagtt gcaatacagc gatgagctgt
     4201 acgccgaaac cccgtgggct gacgatgtga aagcgtttct ctgttacaac gccaataagg
     4261 ctttgatgaa tctgggctac gaaccgttat ttcccgcaga aatggcggaa gtgaatccgg
     4321 caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca cgatttcttt t
//
LOCUS       DAJVTY010000226         4309 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVTY010000226 DAJVTY010000000
VERSION     DAJVTY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.227"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..965)
                     /locus_tag="OQQ66_003865"
     CDS             complement(<1..965)
                     /locus_tag="OQQ66_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1906604.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELA"
     gene            complement(1120..1746)
                     /gene="dsbA"
                     /locus_tag="OQQ66_003866"
     CDS             complement(1120..1746)
                     /gene="dsbA"
                     /locus_tag="OQQ66_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1906605.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1763..2749)
                     /gene="srkA"
                     /locus_tag="OQQ66_003867"
     CDS             complement(1763..2749)
                     /gene="srkA"
                     /locus_tag="OQQ66_003867"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1906606.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2826..3095)
                     /locus_tag="OQQ66_003868"
     CDS             complement(2826..3095)
                     /locus_tag="OQQ66_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1906607.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3165..3749
                     /gene="mobA"
                     /locus_tag="OQQ66_003869"
     CDS             3165..3749
                     /gene="mobA"
                     /locus_tag="OQQ66_003869"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1906608.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3731..4258
                     /gene="mobB"
                     /locus_tag="OQQ66_003870"
     CDS             3731..4258
                     /gene="mobB"
                     /locus_tag="OQQ66_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1906609.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1166 a         1017 c          965 g         1161 t
ORIGIN      
        1 tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata ctggatgata
       61 aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc accactaccg
      121 agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac attgatataa
      181 tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat ttgggcaacg
      241 tcttccccct gattgataat attaacctga ttaaatttcg actcgctttc accaaccgga
      301 aatttctttt cttctaatcg tgtcagagaa tcaatggtaa aactttttgc cgttactttg
      361 ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc caggttagac
      421 aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa ctttccatac
      481 tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc
      541 gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ttcattattc
      601 actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg
      661 atgggcaacg gaccgtgctc aacatccaca tcaaatgcaa ctttctggcc tggcttgata
      721 ttgagatcgg gtgcaccgtt atcaaatgaa atttgcattt gaaaatggga attaaaaaag
      781 cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc tttcttttta
      841 gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt acctgtgtac
      901 catatgccac caccccagat tacggctaac gcaacaataa cacctgtagc tgacttacgt
      961 atcatataaa tattccctga taaaacagca aaactttgaa tatccactta tgctgaaata
     1021 atgtccagcg gcaggatgta ttatcagaat gtatttatat ttattaaata ataaaaaaag
     1081 cccgtgaata ttcacgggct ttatgtaatt tacattgaat tattttttct cggacagata
     1141 tttcactgta tcagcatact gctgaacaaa aacatccata ttgctggtat ccataccctg
     1201 cggattcagc tgatatttac cgttaacaaa catcgccgga acaccgcgca attgcacgtc
     1261 agctgcagct ttttcctgct gagctaccag agatttcacc acgaagctgt tccacgccgc
     1321 gtcgtactct tcacctttaa tacctgcgtt gataaataca tcgcggatat cagaagcaga
     1381 acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt tgtcttccac
     1441 gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt caccacccat
     1501 gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct tcacattatc
     1561 agaaatatgc agaacttctt caaactgata gcagtgcgga cagaagaaag agaaaaactc
     1621 cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt taccatcttc
     1681 atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca gccaaatctt
     1741 tttcatgatc tactctctcc gattaataca taggtgttaa ttgtaaaggg ggttcttgta
     1801 gaacttttgc ctgttctata aaagtcgccg tttgtcgcag ccagtaatct tccccggtta
     1861 accacgggaa atttttcggg aacgcgggat cagcccaacg ccgcattagc caggcaagat
     1921 aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact
     1981 cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc tcggctttat
     2041 cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca tcatccagat
     2101 caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct ccatgtaagc
     2161 gcagaacggt ggaatcttcc cgccagtgcg ctgtaacggc ggcgatcagc tcatctgtcg
     2221 ctttcaggaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca aacagcttgc
     2281 gtggctcaat gagatattca ttcagaccga tggtcgggcg atggataaaa agctgtttgc
     2341 gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga tcgatattat
     2401 cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat
     2461 tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca tcatttacca
     2521 actgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt tcagggcgat
     2581 aaaatttgac gacaatacgt cgacgatctt cgtcctgaaa ttgatagaca cggttttcat
     2641 agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaat agagcatcca
     2701 tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc ccatcatccg
     2761 gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc gccgcttaca
     2821 agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca
     2881 taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat tttcaggtga
     2941 tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga ctctttcgcc
     3001 agtttctgca aaaattgcaa caggttaagg tctggctctt tttgccaggc tggctggagg
     3061 aattcaataa cttcattcag acgtttacat ttcatagtga tgctccttac tcttgagaca
     3121 gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat ctgatgacga
     3181 cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggtggc gtagataaag
     3241 gattgcttga attaaacggc aaaccattat ggcaacatgt cgctgacgcg cttatgacgc
     3301 agctctctca cgtcgtggtt aatgccaatc gtcatcagga aatctatcag gcaagcggtc
     3361 tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga atgctttcag
     3421 taatgcagca ggaagctggt gagtggtttt tgttttgccc gtgcgatacg ccttacattc
     3481 cccctgattt agcagcccgg cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc
     3541 atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt gagcctttat
     3601 tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt ctggctggcg
     3661 gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat acgccagagg
     3721 agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg cgtggagtgg
     3781 taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca gagggatccg
     3841 tccagggctg attaagcata cgcaccatga tatggatgtt gataagccag gcaaagatag
     3901 ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc aacgatgggc
     3961 cttgatgacg gaaacaccag acgaagaaga gctggatcta cagtttctcg caagtcgaat
     4021 ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag agatcgcaaa
     4081 gattgtgctg tttcgcgatg gagccagaca tcgacctgaa gaattagtga tagacaggca
     4141 tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac tggatattaa
     4201 tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga acggataagc
     4261 aagagtaggc tggatcagac gcagccagtg tcgcatccga cctttttct
//
LOCUS       DAJVTY010000227         4223 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVTY010000227 DAJVTY010000000
VERSION     DAJVTY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.228"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(669..898)
                     /locus_tag="OQQ66_003871"
                     /pseudo
     CDS             complement(669..898)
                     /locus_tag="OQQ66_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1485..2008
                     /locus_tag="OQQ66_003872"
                     /pseudo
     CDS             1485..2008
                     /locus_tag="OQQ66_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(2325..3590)
                     /locus_tag="OQQ66_003873"
     CDS             complement(2325..3590)
                     /locus_tag="OQQ66_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1906610.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
     gene            complement(3837..>4223)
                     /locus_tag="OQQ66_003874"
     CDS             complement(3837..>4223)
                     /locus_tag="OQQ66_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906611.1"
                     /translation="FVFDSSEYRYALNMREVVFHQFLSDGLRPIVDEDDDAYEDDYFN
                     VHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFDALDCEPETTDRFMITDEDGED
                     AFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT         1138 a         1033 c          931 g         1121 t
ORIGIN      
        1 tagcttacgt tatagctgtt ttcctttgca gtatttcttt gcttttctgt atacctttat
       61 acctgttata ccagatcaaa aaacaagcaa tccacataac aaaacgcgtt ttgttactga
      121 tgtcacaaat tgagcatatt tttgtagctg atagtttgtt acaaacacca gaaccaagat
      181 gcctatttcg tttcagcatc aactgttaaa ttatctggct gccaacgaga atactgagct
      241 tttttgtccc caagactgac cgtcaacgcc acaatctttg actgtttgac attgtcatgc
      301 ccgcaggcca gcggatctaa agacattaga ctgcttgcat cagccagttg cgtatctggc
      361 tatgtccctt aattggtaag ggttcgagga actcaacatt aacgcatttt tcagcgcatc
      421 accgacagtg tatatgaacg attgcactct gacgatggtt gatttatttg gttgcgtagg
      481 ggctagctag tctggtaccg gaggctggat ctgaagtact gaccccagga ataacgccgg
      541 gctgactcat attctgaccc attgtcagcc cgcgtgttta ctgctctagc tcatttcctg
      601 ttgtcaggcg ttcaattccc aactgcaggt tatggcccgg tgcacaaaca cctgacggca
      661 ggtatcactt acccgaagaa ggtaaagccc tcaagaggca gtaactcagc caacctctcc
      721 tccggccact ccggcagacg cgtcaggacg tccgtcagcc aagcatgtgg ctccagactg
      781 cggttcccag aaggctcatt atctgtgccg cgcggttccc ggccatcagt gaaccagcga
      841 acaaccaggc ctttcgtccc ataacgaccg gacggatagc acgctcgcag atattgttgt
      901 gtgtgccttg aaatgcacga tctttccgtc ggtgaaagtc cgccccggct aactctcgct
      961 ccgactggaa ggacaagaag cggtccagga ggtaacaaaa gggctgaatc tctccctgaa
     1021 acgacttctt caggaagtca acgggttatg cgggccgtaa cgtgagtgaa cactgagcag
     1081 ccatcgaaat gccgtcgagg gtgctgacac tccacaataa cggggaaggc cgacgatgac
     1141 cgggaaaaga gtgagaaagg catcggtcat acccaccggg gttgtggtgt cagcatgcat
     1201 aacaattgat cgatcgtgca acaagagaaa tccataacga tgtctgtccg ggtgacgggc
     1261 aacaacgcct cgcgaggggg tgaatcggtt gttatggatg gcagatgggg tgtagtagtg
     1321 aaaatactga gtaatgtcag aggagcgaag gccccctctt gcagtaccag tgtaacatgc
     1381 aggaagagcc cggggattgc catcagcctc acaaccccgc tcgaaccggt tcagaaccta
     1441 cagaaaacac tacaggagaa agcaaagtga accgccccgt gtttcctgtt tatcattttc
     1501 tggtcagtgc cgcgattctg gtattcgtgg tgattttctg gcggacacac catcgcgacc
     1561 accgtaactg gctggctcta cggctattcg tattatgctc tgtaaaccgt tggccattgc
     1621 ggatggttaa aggaacagtt gtggggacaa ctgacacatt acgtgaaatg cagcgttatg
     1681 aacaactgag tcagcacggg gctgacaact ggaaaatcca gccgggagca ccgttatatg
     1741 atacgattgt tattgtcact ggtgagagtg tgcgcaggga ttatatgtca gtgacataac
     1801 gtctcatcag cccgtcagta gaaaaatcag tgcccgtcat ttcgcaggta tttttcagtg
     1861 gatgaccggt atttgtactg aaaatatacc gccattcaat ccgctgacag acgaagataa
     1921 ttaaactgtt atggttttta agggagagag gaatataccg gcagacagtt tgaaacctca
     1981 gccacttatt cttcctgatc acaggtaatc atatatggca gactgtaata cagtcgcttg
     2041 tactgaaaag tattctgtat aaaatatccg tattcatatc agcacaaggg accatcaaat
     2101 ccctgcagca ctccttacgt cagttatccc gcatcaccct gtgagtacag gattttttat
     2161 gaggttactt ttttattgaa agtatataaa taatgaataa ccgcggattc tgataatccc
     2221 ccctgcaggg agcggtaaat agtaaataca tcagttaata ccgtttgtct tttttaacaa
     2281 agaaaataat cctagaataa ttcccgggca tttgcccggg atgattactg ttttaatgga
     2341 ttattaatct ttgcatattc aaatggactg ataaatcttt ctctgtttac atccagaaaa
     2401 tcggcccacc actgtagcat caatcgccgt tcttccagat gctctgcttt atggatatac
     2461 gcggccctca ctgaatttcg ctccatgtgg ctcatctgac gttcaacagc atcacgagac
     2521 cacaaacctg attcgaccaa tgaactacag gccattgttc gaaagccatg accacaaacc
     2581 tctacttttg tatcataccc catgacccgt aacgcactat ttaccgtatt ctcactcatg
     2641 ggtttgtgcg aatcgtgatc accaataaat atcaagtcat gggccccata aaactgtttt
     2701 atctgcttta aaattgcaag agcttgcgtt gaaagaggca ctagatgagt tgtacgcatt
     2761 tttgagcctc tatgggaatg tttcactcca ggaataggct cccgctccgg tgggatagtc
     2821 catatagacg cttcgaaatc gatctctgac caacgagcaa aacgcagctc actggaccga
     2881 ataaagatca gcaaagtgag ttctatcgcc catcgggtta gcggcctacc agtatagcta
     2941 tctatttttg taagcaactc agggatgcgc tttaattcaa gcgcgggacg atgttgtcga
     3001 ttacaggaag caaccgcccc agccatctct tgtgccgggt tataatcaat taacccactt
     3061 tgcactgcat agcgcatgat ggctgtagtg cgctgctgaa gacgagcggc cacttcaaga
     3121 cgtccagaca tttctacggc cttaataggt gctaataaat ctcgagtttt taactcagcg
     3181 atattacgtt caccaagcgc tgcaaaaaga ttatcttcaa gactttttag cacacgatgg
     3241 gcgtgatctt cagaccactt tttattggtg ccatgccact caatcgcgac ttctttaaag
     3301 gttcgtgctt tactctgttc aaccttatca tttttctttt tgtctcccgg atcgacgcca
     3361 ttcgcaagca ggttacgcgc ctcgtcacga cgtactctgg catccgctag tgtgatttca
     3421 ggataaaccc caagtgccag cattttttgc tttccctcat aacggtactg caaacgccag
     3481 tacttagaac catttggatg gacaagcaga tgcataccgc caccatcagt cagcttatat
     3541 tgcttatccg ttggcttggc tgctctgact ttgatatctg ttaatgccat gcgtattccc
     3601 tccctgttgg tacaagcatt atcgaaccgg aaataccatc atctgtacca acatatgttg
     3661 gtagatgtac gttgatatcg gttgaccttg agagagttaa tatagcggga aagatagata
     3721 aacactggat ttcaggcata aaaaaagacc tcagttgagg tctatttaca tacttttggt
     3781 gccgaaggcc gcaccgcgat tccagactca acaatatgaa atcaatccat tcaatactac
     3841 tcatcatcgt cttccactgg ttccagcaca tcaagcgcaa ctcgcaccat agcgatagaa
     3901 ccaatacgaa tgaaagcatc ttctccatct tcgtcggtta tcataaaacg atccgtggtt
     3961 tcaggctcgc aatccagcgc gtcaaaaaat gcattcaact ggccgatatc atccgtctct
     4021 tcgttttgcg gcgcatctgg ctcaacactc agtggaatca ctggtccgcc gtttaccagc
     4081 gtgatatgga cattaaaata atcgtcttcg taggcatcat cgtcttcgtc aacgattggc
     4141 cgcagaccat cagaaaggaa ctgatgaaat accacttcac gcatatttaa cgcataacga
     4201 tactcggaag aatcaaaaac gaa
//
LOCUS       DAJVTY010000228         4183 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVTY010000228 DAJVTY010000000
VERSION     DAJVTY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.229"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ66_003875"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ66_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1906612.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQQ66_003876"
     tRNA            complement(324..399)
                     /locus_tag="OQQ66_003876"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQQ66_003877"
     tRNA            complement(406..480)
                     /locus_tag="OQQ66_003877"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQQ66_003878"
     tRNA            complement(597..681)
                     /locus_tag="OQQ66_003878"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQQ66_003879"
     tRNA            complement(690..765)
                     /locus_tag="OQQ66_003879"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQQ66_003880"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQQ66_003880"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1906613.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQQ66_003881"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQQ66_003881"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1906614.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQQ66_003882"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQQ66_003882"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1906615.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1078 a         1041 c          933 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJVTY010000229         4177 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVTY010000229 DAJVTY010000000
VERSION     DAJVTY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.230"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..642)
                     /gene="exoX"
                     /locus_tag="OQQ66_003883"
     CDS             complement(<1..642)
                     /gene="exoX"
                     /locus_tag="OQQ66_003883"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1906616.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLK"
     gene            complement(666..1322)
                     /locus_tag="OQQ66_003884"
     CDS             complement(666..1322)
                     /locus_tag="OQQ66_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS1906617.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1424..1654)
                     /gene="holE"
                     /locus_tag="OQQ66_003885"
     CDS             complement(1424..1654)
                     /gene="holE"
                     /locus_tag="OQQ66_003885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1906618.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            1793..2167
                     /gene="yobA"
                     /locus_tag="OQQ66_003886"
     CDS             1793..2167
                     /gene="yobA"
                     /locus_tag="OQQ66_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1906619.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2171..3038
                     /gene="copD"
                     /locus_tag="OQQ66_003887"
                     /pseudo
     CDS             2171..3038
                     /gene="copD"
                     /locus_tag="OQQ66_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            3051..3392
                     /locus_tag="OQQ66_003888"
     CDS             3051..3392
                     /locus_tag="OQQ66_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1906620.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(3774..>4177)
                     /locus_tag="OQQ66_003889"
     CDS             complement(3774..>4177)
                     /locus_tag="OQQ66_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1906621.1"
                     /translation="DDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGD
                     YLISAGIRKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEVRSLAGRLYKETC
                     GEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         1043 a         1009 c         1030 g         1095 t
ORIGIN      
        1 tttcagtgtt aaacgcagct ccgggctcat gctgtccagg ttattaaatg accagcgcag
       61 atagcccgga tcgcgttcgg caacgtcgga aaccgctttg ccacggtatt tgccaaaggt
      121 gaaggtcgtc atcaacgacg gacgtccggt gatatcggcc atctgttctg ccgtccagcc
      181 ggaggtgttc ataatatcga taagcaacgc ggcggtgata taacagtcat acaacgcgcg
      241 gtgatgatgc aggcccggcg gcgtctgtac attgagcttc cgtgttttat ataacgccat
      301 attgctgtac ttgatccctg gccacaaacg acgggccagt ttcatagtgc aaatccactc
      361 accgggcatc tcaggcagta cgcggcggtc aaagctggcg ttgtgcgcga cataccattc
      421 actaccgtaa tagtgtggga tcacatcttc aatccacggt ttatcggcga ccatggcttc
      481 ggtgatgcga tgaatcgcca tcgcttgtgg actaatagga cgatcggggc gcaccaggtg
      541 gctcatgggg ttgacgattt ttccgtcaat gacatcaaca gaggcaatct caacgatccc
      601 tccctgcaaa ccgcaggttt ctgtatcgat aatgcgcaac atggcatgct ccaggccgaa
      661 aaagcctagc gtaatggaat gatatcgcct tgccaaccct gtgatgaacg ctgaccgact
      721 aacaataatg aaccacgatc ggcgcgaacg atgagacgac catattcatc ccatagtgca
      781 ctgttaccac gggcatttgc catcagcacg gcaatcgagt actgatgtga aaaaaattgt
      841 aagcgacggg ccgacgccag caggtcgggt tctcccagac attgccctgt agtaaacagt
      901 gaacaggtcg ggtccatatc cataccttgc ggttgttcat cgaccaccgt gatggttctg
      961 gaacgacggc ccagacatgc accatgactc tgatggtaga tcccaggcgt ttttcgccac
     1021 ggtgcgaaca ccgcaattcc acgaataaag cgatcgttat attcaacggg aaggccggca
     1081 atgatggtca ttcgccaggt cgttgcggca taacaaagcg ggtctaacag tgagagatcg
     1141 ggtggggcag gaagggcacg tcgcgaataa tcacacgcca gtaaagaaag cgatggaaaa
     1201 actaacagct gacattgttg cctggcggcg gccctcacaa actccagatg atgagctacc
     1261 tgttcggtga gtgaggtttt acgaggctca tattgcgctg ctgcaacttt ccagaacgac
     1321 ataatgactt ccttttcaaa atgactgcaa attatgggtg cggtttagaa aatactcata
     1381 atttagcagg cgttatgtaa gaaagtgtaa ctctgtataa gttttattta agtttgggct
     1441 cgtaaggtaa acgtgacagt ttgaccgaag ccaaacggtg ggcaataagc ctctcgcgaa
     1501 accagctgcg caaatgttca ggctgttcac gttcaaccgc ttcagcgatc accggcatat
     1561 tgtagcgttc tttaaatgcc accccggccg ccgccaaatc gacattcact ttatccattt
     1621 ctgtttgatc cagtttagcc agattcttca gcattcttaa tctcctcatc attcgcgggc
     1681 aaaagttact aagagtgagc caggatggca agccggaatt gatgatcctc gactcagaca
     1741 ttagacaagg ttatgctctg taaaaaagtt attacaaaaa gtaatagata atatggcttc
     1801 aactgcacgc tcccttcgct acgcgctggc aatcctgacc acttcgttgg tgacaccttc
     1861 cgtctgggca catgcgcatt taacgcatca gtatcctgcg gcaaacgcgc aagtgacagc
     1921 tgcaccgcag gcaatcactt taaacttctc ggaaggtgtt gaaacaggat tcagtggtgc
     1981 aaaaatcacg gggccaaaaa acgaaaatat taaaacatta ccggcgaagc gaaatgagca
     2041 ggaccaaaag caactgattg ttccactggc cgattcgctg aaacccggaa cctacaccgt
     2101 tgactggcat gttgtttcgg tggacgggca taaaacgaaa ggacactaca cctttagcgt
     2161 gaaataaatc atgctggcgt ttacctggat cgcgctgcga tttatccatt tcacctcgct
     2221 gatgctggtt tttggcttcg caatgtacgg tgcctggctg gcccccttga cgattcgtcg
     2281 cttgctggcg aagcgttttc tgcgtttaca acagcacgct gccgtctgga gtttgatcag
     2341 tgccactgca atgctcgccg ttcagggcgg gttgatggga acgggatgga cagatgtatt
     2401 ttcgccaaac atttggcagg ctgttttgca aacgcagttt ggtggcgtct ggttatggca
     2461 aattgttctc gccctcgtaa cgttgcgccc ttatgcaacc gcggaatatg ccacgtttgc
     2521 tgtttatgct cactaccgcg caatttattc tgctggcggg agtggggcat gcgacgctga
     2581 atgaaggggt aacggcgaaa atccatcaga ctaatcacac gattcacctg atttgtgcgg
     2641 cggcgtggtt tgggggatta ctgccggtgc tctggtgtat gcaactcatc aaaggccgtt
     2701 ggcgacacca ggctattcag gcgctgatgc gtttttcatg gtgcgggcat tttgcggtga
     2761 tcggcgtact ggcaagcggg gtgcttagtg cattgttgat taccggattt ccccccacgc
     2821 ttacgactta ctggggccaa ctcttattgc tcaaagccat cctggtgatg attatggtgg
     2881 tcattgcgct ggctaatcgt tatgttcttg taccgcgcat gaggcaggat gaagatcgtg
     2941 cggcaccctg gtttgtgtgg atgactaaac tcgaatgggc gataggtgcg gtagtgctgg
     3001 tgatcatcag cctgcttgcg actctcgaac ctttttgatg gattacgaaa atgatgaaaa
     3061 aaagtattct ggcgtttctg ttactcacca gttctgcagc ggcgctggct gcaccgcagg
     3121 tgattaccgt cagtcgtttt gaagtgggta aagacaaatg ggcgtttaat cgcgaagagg
     3181 tgatgctgac ttgccgaccg ggtaatgctt tgtatgttat taatccgagt accctcgtgc
     3241 agtatccttt aaacgatatc gcacaaaagg aagttgccag tgggaagacc aatgcccaac
     3301 ccatttcggt gattcagatt gatgatccta acaatcccgg cgaaaaaatg agtctggcac
     3361 cgtttataga acgagctgaa aaactctgtt aattacctaa aatagccttt tgatttccaa
     3421 taaaaaaacc gcctcagttc tttcaccaga acgggcggtt ttaacatttc agctgatgac
     3481 caccacgctt tttattgacc attttgcacg caaactggaa aacctggcgt cgtcatctat
     3541 tcttaaaggg caaggcaact aagcctgcat taatgccaac ttttagcgca cggctctctc
     3601 ccaagagcca tttccctgga ccgaatacag gaatcgtatt cggtcttttt ttaattgcat
     3661 ttaaaatcaa ttagttgcaa atgtctcccc gaaatttact cgaatttctg tattccggtc
     3721 ttttttggtt atatcacaac caaaatacat ttaacaatcc atttacgtta aaatcagagc
     3781 agtaagtacg ttttttctct ctcatcaaga tacatttttg ttgtcttctc cgatgtgtgg
     3841 ccaagtagac gctgagcaaa ttcttctcca catgtttctt tgtacaatcg tccagccagg
     3901 cttctgactt cgtgaaaagt tggtgggttt tcactgaact ggatacctgt taattttctg
     3961 gctgcgacaa atttctttgt caggccgtcc gggtgaatgc tgccgtcagg gctgtttttt
     4021 ctaatcccgg cactgattag ataatccccc ctacttacca tgcggcactg ttcaactact
     4081 gcaccaagtc gtaaaccagc gacaggaagg ctgagtgaca gggggatagc aatcatcatt
     4141 cctgtcttaa tttgcctgat gtggagacga tcatcat
//
LOCUS       DAJVTY010000230         4169 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVTY010000230 DAJVTY010000000
VERSION     DAJVTY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.231"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..154
                     /locus_tag="OQQ66_003890"
     CDS             <1..154
                     /locus_tag="OQQ66_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906622.1"
                     /translation="CQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTE
                     MIQATA"
     gene            complement(194..1408)
                     /gene="ydcO"
                     /locus_tag="OQQ66_003891"
     CDS             complement(194..1408)
                     /gene="ydcO"
                     /locus_tag="OQQ66_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCS1906623.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1461..1997
                     /gene="sutR"
                     /locus_tag="OQQ66_003892"
     CDS             1461..1997
                     /gene="sutR"
                     /locus_tag="OQQ66_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCS1906624.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2070..4031
                     /gene="rlhA"
                     /locus_tag="OQQ66_003893"
     CDS             2070..4031
                     /gene="rlhA"
                     /locus_tag="OQQ66_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS1906625.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
BASE COUNT         1088 a         1066 c         1074 g          941 t
ORIGIN      
        1 atgccaggta tgtggatata cagcgaacgc cgatgtaaat ggcgctcgta acattttagc
       61 ggcggggcac gccgttcttg cctgtggaga gatggtgcag tcaggccgcc cgttgaagca
      121 ggaacccacc gaaatgattc aggcgacagc ctgaacgtag cagggatcca cgtctttcag
      181 ggcgtgtagg atgtcaatat tggtttctgt cagcgattaa attcaacacc acgtaacaaa
      241 cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc aatccacttg
      301 ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc agcgcctgat
      361 acaaactgcc gccaatggta ctcaacagcg ccagacctgc cagcatctgg atccaactta
      421 cgggcagggc agccatcatc tcggtaatgg cactaccaaa cagacctgcg agcaaataga
      481 aaatgcctgc aacggcagct gccagccaac gttgatcttt atccggatgc gcttccgggc
      541 tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg aaaggggaaa
      601 aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga aacaggagcc gaatatccag
      661 ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc caccgtcacc agaaaaaggg
      721 ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa gtggggagaa
      781 cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg cgcgatgacg atcacgatcc
      841 caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggtt gccagccata
      901 ccagcaacat acttccacac aacgtaaatt gaccgtccag actggcaaac gcctgtaaac
      961 caaagcgtaa taaaatctcg gcaagcattg ccgccgcaag cgagtgcgga ataatgcgca
     1021 tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg gtgacaataa
     1081 aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac aaagccgcgc
     1141 caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga gtcagcgtac
     1201 tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca gtggtggctc
     1261 tggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca attaataccg
     1321 ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa cagacgcata gtcacctcgt
     1381 acgttatagc gtacaggcac cgtagcattt gtccgttata acgcacaagt gataaacttc
     1441 cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt tttatccacc acacttaaac
     1501 aactacgcca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg ggcgtttcta
     1561 aagcaatgtt agggcagatt gagcgtaatg agtccagtcc gacggtcgcg acattatgga
     1621 aaattgccac cgggttgaac gtaccatttt caacatttat ttctccgccg cagtccgcta
     1681 cgccttcagt ttatgatcca caacagcagg cgatggtgat cacttcgctg tttccttatg
     1741 atccacaact ctgctttgaa cacttctcga ttcagatggc atctggtgca ataagcgaat
     1801 cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga caacttgatc
     1861 tgtgcgttga tggggaatgg cagaccctta attgtgggga aggcgttcga tttgctgcag
     1921 acgtcacgca tatctatcgt aatggtgggg agcaaaccgt acattttcat tccctcatcc
     1981 attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg gctaaaatag
     2041 ccgccatttt tcagctactg gataagaatg tgaccgtatc ttctcatcga cttgaactgt
     2101 taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt gccgatgctg
     2161 tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc ctgaaagata
     2221 ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc acgcttaaca
     2281 ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac ctctaccaga
     2341 ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat attccgccga
     2401 ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg aagttcctct
     2461 ctgatgttgg cttcacgcag attgtgctgg cgcgagagct gaatcttgat cagatccgcg
     2521 cgattcacca ggctacggac gcgaccattg aattctttat tcatggggca ctgtgcgtgg
     2581 cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc aaccgtggcg
     2641 attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg cgggtggttt
     2701 cctatgaaaa acatctgctg tcgatgaaag ataacgatca gactgccaac ctcggcgcgc
     2761 tgattgatgc tggtgtacgc tccttcaaga ttgaaggtcg ttacaaagat atgaactacg
     2821 tgaagaatat caccgcccat tatcgccaga tgcttgatgc cattattgaa gaacgtggcg
     2881 atctggcgcg cgcttcatca ggtcgtactg aacatttctt tgttccatcg acggaaaaga
     2941 ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat attggcgcgt
     3001 tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt attgaaagtg gcgaaagatc
     3061 atctcgatgt tgccgtcacc gagccactgg caaatggtga tggcctgaac gtgatgatta
     3121 aacgtgaagt catcggtttt cgtgccaata cggtcgagaa aaccggagaa aatcagtacc
     3181 gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat tcgtccacat cacccactaa
     3241 accgtaatct tgatcataac tggcagcagg cactgacaaa aacctccagc gaacgtcggg
     3301 tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc ctcaccagtg
     3361 aagagggtgt cagcatcacg catacgctgg acgggcagtt cgacgaagcc aataacgccg
     3421 aaaaagcaat gaacaatctg aaggatggtc tggcaaaact ggggcaaacc ctctattacg
     3481 cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt ctgttaaacc
     3541 agttccgccg tgaagctgct gacatgctgg atgctgcgcg tcttgccagt taccagcgcg
     3601 gcagccgtaa accggttgct gagcctgcgc cggtttatcc gcaaacgcat ctgagtttcc
     3661 tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt gtgcagctga
     3721 ttgacgcggc gtatgaggca catgaagaga agggcgaagt cccggtgatg atcaccaagc
     3781 attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat atcaaaagct
     3841 ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta aagtttgatt
     3901 gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg aaaatgccgt
     3961 taccgggaag cgtagtggca tccgtaagtc cggatgagct gctgaaaaca ttgcctaagc
     4021 gaaaagggta aaacgccagt tttctggtta ctcacaactc attgaatctg catgatattg
     4081 cctgccgggt aaggcgtcac gccgcatccg gcatcaaacg actcaacgtt gtcccgcttc
     4141 tggtttgcgc gatttttgcc agtaaaaat
//
LOCUS       DAJVTY010000231         4116 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVTY010000231 DAJVTY010000000
VERSION     DAJVTY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.232"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(26..583)
                     /gene="ymfL"
                     /locus_tag="OQQ66_003894"
     CDS             complement(26..583)
                     /gene="ymfL"
                     /locus_tag="OQQ66_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCS1906626.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(627..827)
                     /locus_tag="OQQ66_003895"
     CDS             complement(627..827)
                     /locus_tag="OQQ66_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906627.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            918..1592
                     /locus_tag="OQQ66_003896"
     CDS             918..1592
                     /locus_tag="OQQ66_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCS1906628.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPEVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3121..3657
                     /locus_tag="OQQ66_003897"
     CDS             3121..3657
                     /locus_tag="OQQ66_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCS1906629.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3648..4010
                     /locus_tag="OQQ66_003898"
     CDS             3648..4010
                     /locus_tag="OQQ66_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906630.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
BASE COUNT         1254 a          896 c          873 g         1093 t
ORIGIN      
        1 cgacggtagt gtgttgttaa actgttcatg cgttagtttc tccacaacca gaagcaatcg
       61 acgccacgac gcccggagct gcacactcgc gggcgtcatt actttctgaa atgcaaaaaa
      121 ttttgtagac aagtgctgca tgctcctgca gcttcgaaat tgagagatac agctcgtcgt
      181 taattgctgt cttctcatgc ggttccacta caccgtcttc gattgctgaa cgaatctgtt
      241 ttgaataact gccgatctgt tcaatgactt ccagcagacg ctggttaata tcggcgttgt
      301 ccacatcctc gatgtcagga agagacacaa agacgccatt tgcagactgc gccacagcat
      361 cagcaatgaa gtgagtgcca ccagcacgct gtaaaaccat tgcccatccc agcgggaaaa
      421 tctgatcgcc atctgcacga aggcggttga ataaagcgtt ttctgttaca tcgagccagt
      481 cagccgcttc agcgtaacca cccggcaact ttgcgatagt ttttctgaca gctttcacgt
      541 accactcagg ctgtttttct attttccagt gatgcttacc cacgattagc ctcatcgttc
      601 tgtggttaaa aattgaaagt gttctgctaa tctttcggat agatatccgg tcttaagtca
      661 gatttcgtaa ttgcacctga cgtgcattgc tcaagttttt tagccagcac aaaactggct
      721 tttttatagc cattaaaaac cagccgtaag tagccttgtg ttgagccaac ttttccggcc
      781 aactcaccct gctgttcttt ggttaaagag tcccaatacg ctttcataca atatgtacct
      841 ccagtgtaca catcacatga ttgaaatgaa ccttcaagat acttgtacct tagcggtaca
      901 agggttttaa tttcgttatg aaaacaatcc atgacatccg gcggtctaac gccagaaaac
      961 tgagagatgg tgttggcggg aattcttcct ttgccactat gattgatcgc gagccaaccc
     1021 agaccagcag gtttatggga gatggtgcta ctaaaaatat cggtgacagc atggcacgac
     1081 acattgaaaa atgtttcgac ctgcctatcg gatggctcga tcaagaacac cagacaacga
     1141 acatcacaaa aaaacctgaa gtttcaatca ctaacaaaca aataacgtta gtccctgtca
     1201 tatcatgggt acaggccgga gcatggaaag aagttggcta ttctgaggtt gatttgagca
     1261 cagcagaaac ttatccctgc cctgtaccct gtggcgaaat gacttatatc ttgcgggtga
     1321 ttggtgattc aatgattgat gagtaccgtc cgggagacat gatttttgtt gatcctgaag
     1381 tccctgcttg ccacggtgac gacgttattg cgttgatgca cgatacaggc gaaaccacct
     1441 tcaagcgatt gatagaagat ggaacacagc gttatctcaa agcattacac ccaaactggc
     1501 ctgagcccta cattaagatt aacggtaatt gctctataat tggcactgtg attttctcgg
     1561 gaaaaccaag aagatacaaa ataaaggcct aatcaatatt tatgaacctg cttcggcagg
     1621 tttttttata cttgacaatg taccccatgg acacacaatg taccccatgg acacacaatg
     1681 taccaaaacg aaacaacaaa caggcaggat gcccacgaag tagccgcctg gggcatatga
     1741 agtccaagat gattcgttag caacaaaacg cccataggac gcttcgctct ttaacaattt
     1801 gaatattttc agcgtcaatg atctgtttag ctccctacaa gtaaatgtag cgtagcacca
     1861 tgataaatcc tgctagtgtt tagttaaaaa tcgcgcgaga gcaagaacga cgtgagggca
     1921 ggcaatactg atagtgttat tgctattagt ttatcaaaaa tatttagacg acccagatga
     1981 atatttttaa tacatactcc ccctaattta acaggagtaa atgttgatga ataacgagac
     2041 atctatactc aacaattgta tgatatgaac aattatataa cataatgtag gaaaaataaa
     2101 aatggattgg catgacctta tatcaaaggt aattatcgga cttgttacag gctcaatcgc
     2161 agcttactta actgcgcgat atgcactcgg ccgttattat aaagaaaaat ggtgggacaa
     2221 aagaatagat ttgtatacgt aattagttga ctgtgtttat cttctaaaaa acacagctga
     2281 ctattggtat aaacatgagt tacatcacgc ttcacaattc ccaacctact ttagttacaa
     2341 atcaaaagaa gaaaatgaaa tccttagcaa atctttctct aatgagcttg aaaaactcag
     2401 tagaataggt gagctatctt cattattctt aacagaagag tgtggggata aaatccgttc
     2461 attcattgaa aatgaagaaa atctatttat acaatgggtg tttgatagta tagagttgat
     2521 agatgcccac aagtcaagac ttgataaatt aaaagtatta ctagacgata ttatctctga
     2581 agcaaagaga gagcttaagg tcaataaaaa acataacagc ctaaagaata taaagtggtc
     2641 cataactatg ccggaccaaa aaaatgatga gccaattaat tatcacgaca atcattaaaa
     2701 cactttagaa cccgctgggc gggttctatt caaatatact ttgcacatca caaataaaac
     2761 tataacgtcc ttcttctttt cctaatatct tatatctact tatttataag cggcttacag
     2821 atcttcaaca gataaaccta atgaaggcga caatgttctg atgaatagcc acctttttac
     2881 tcaactgaaa gcgcgttctg tcccttgtca ttaagtgcca gttcgttaag tccaaaacct
     2941 ccggaacgcg ctttcaattg agtggggaaa ataactggcg atggcagccg cccgttgcac
     3001 taagtgcctc tggatggggc atttactgaa acgaaaccaa aacttttaat cgccttatgg
     3061 cgagggattc gtgcaaccaa aaatcgtgcg gtgcagcgca ccaattaagg agaactaacg
     3121 atgagtttta tccagactct taccggtaag aagttcaatt atatcaactc caccattgac
     3181 gatatttata tcgaggatat cgccggtgcg ctttcaaata tctgccgctt cgcgggacac
     3241 ttgccggatt tttacagcgt ggcgcagcat tccgttctgt gcagtcaact tgtggcgcca
     3301 gagttcgcct ttgaagcgtt aatgcatgat gcaacagaag cgtattgtca ggacatcccc
     3361 gcgcaactga aacgccttct tcctgactat aaacggatgg aagaaaaaat agacgccgta
     3421 atccgtgaga aatacgggtt acccccggtt atgagcacgc ccgtgaaata tgccgatctc
     3481 atcatgctgg caaccgaacg ccgcgatctt gggcttgatg atggctcttt ctggcctcta
     3541 ctagaaggta tcccagcaac agagatgttc aaagttattc cactgtcgcc aggccatgcc
     3601 tacgggatgt ttatggaacg ttttaacgaa ttatcggagt tacgcaaatg cgcatgaatg
     3661 ttttcgaaat ggaagggttt cttcgcggga aatgtgtacc gcgagatctg aaagtgaatg
     3721 aaacggatgc tgaatacctg gtgcgtaaat tcgatgcgct tgaagctaaa tgtgcggcac
     3781 tggaaaacaa agtaatacca gtgtcaactg aactgccacc agcaaatgaa agtgttttgt
     3841 tattcgatgc taacggagaa ggctggctaa ttggctggcg ttctctctgg tacacctggg
     3901 gacaaaaaga aaccggagac tggcagtgga catttcaggt cggggacctt gaaaacgtca
     3961 atatcactca ctgggcagta atgccaaaag caccggaggc tggagcataa tgaccacttt
     4021 taccgacaaa gaactgatta aagaaattaa agagcgtatc agcagccttg acgtgcgaga
     4081 cgatattgag cgccgtgctt atgaaatcgc actcct
//
LOCUS       DAJVTY010000232         4060 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVTY010000232 DAJVTY010000000
VERSION     DAJVTY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.233"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1046)
                     /locus_tag="OQQ66_003899"
     CDS             complement(<1..1046)
                     /locus_tag="OQQ66_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1906631.1"
                     /translation="MREINMLRNISSCLFPHISKITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OQQ66_003900"
     CDS             1537..1647
                     /locus_tag="OQQ66_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1906632.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OQQ66_003901"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OQQ66_003901"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1906633.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="OQQ66_003902"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="OQQ66_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1086 a          828 c          951 g         1195 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattttg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcacctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat
//
LOCUS       DAJVTY010000233         4031 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVTY010000233 DAJVTY010000000
VERSION     DAJVTY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.234"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..213)
                     /locus_tag="OQQ66_003903"
     CDS             complement(<1..213)
                     /locus_tag="OQQ66_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1906634.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OQQ66_003904"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OQQ66_003904"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1906635.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OQQ66_003905"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OQQ66_003905"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1906636.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OQQ66_003906"
     CDS             2894..3634
                     /locus_tag="OQQ66_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1906637.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVITFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3669..>4031
                     /gene="ruvC"
                     /locus_tag="OQQ66_003907"
     CDS             3669..>4031
                     /gene="ruvC"
                     /locus_tag="OQQ66_003907"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1906638.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQ"
BASE COUNT         1000 a          990 c         1135 g          906 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggctaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgagttta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gcagagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 attgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgacagtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc
     3241 attcagcaaa tgtggcggta acctcggtac agatggttcc gtagcctatc tgttcagcaa
     3301 aaaaggcgtg atcaccttcg agaaaggcga tgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg caccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgagattg cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctatggcg
     3721 tcatccgcca ggtaggcagg caactgtcct acctgggtag cggatgcata cgcaccaagg
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc agattatttc gccattgaac aagtctttat ggccaaaaat gccgactcgg
     3901 ctctgaaact ggggcaggcg cgcggcgtgg cgattgtggc ggcggtgaat caggaattgc
     3961 cggtatttga atatgcggca cgtcaggtaa agcaaacggt ggtaggtatt ggtagtgccg
     4021 aaaaaagtca g
//
LOCUS       DAJVTY010000234         3988 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVTY010000234 DAJVTY010000000
VERSION     DAJVTY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.235"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1089
                     /gene="nadB"
                     /locus_tag="OQQ66_003908"
     CDS             <1..1089
                     /gene="nadB"
                     /locus_tag="OQQ66_003908"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCS1906639.1"
                     /translation="VVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSG
                     DGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPD
                     FDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGID
                     LTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECL
                     VYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIV
                     RTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRG
                     LHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OQQ66_003909"
     CDS             complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OQQ66_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1906640.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1943..3277
                     /gene="srmB"
                     /locus_tag="OQQ66_003910"
     CDS             1943..3277
                     /gene="srmB"
                     /locus_tag="OQQ66_003910"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1906641.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3486..>3988)
                     /locus_tag="OQQ66_003911"
     CDS             complement(3486..>3988)
                     /locus_tag="OQQ66_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1906642.1"
                     /translation="CYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLVLVASPQ
                     IADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVA
                     ANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCIE
                     ECFLPG"
BASE COUNT          998 a         1091 c         1034 g          865 t
ORIGIN      
        1 gttgtaggcg cgtgggtatg gaaccgaaat aaagaaacgg tggaaacctg ccacgcaaaa
       61 gcagtggtac tggcaaccgg cggtgcgtcg aaggtgtatc agtacaccac caatccggat
      121 atttcttctg gcgatggcat tgctatggcg tggcgcgcgg gctgtcgggt tgccaatctc
      181 gaatttaatc agttccaccc taccgcactg tatcacccac aggcacgcaa tttcctgtta
      241 acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc cggatggcac gcgttttatg
      301 cccgattttg atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac
      361 catgaaatga aacgcctcgg cgcagattgt atgttcctcg acatcagcca taagcccgcc
      421 gattttattc gccagcattt cccgatgatt tatgaaaaat tgctcgggct ggggattgat
      481 ctcacacaag aaccggtacc gattgtgcct gctgcacact atacctgcgg tggtgtaatg
      541 gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat
      601 accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat
      661 ggctggtcgg cggcagaaga tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg
      721 ttaccaccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat
      781 aactggcacg agctacggct gtttatgtgg gattacgtcg gcattgtgcg cacaacaaaa
      841 cgtcttgagc gcgccctgcg gcgtatcaat atgttgcaac aggaaataga cgaatattac
      901 gcccacttcc gcgtgtcgaa taatttgctg gagctgcgta atctggtgca ggttgcggaa
      961 ttgattgttc gctgtgccat gatgcgcaag gagagtcgtg gcctgcattt cacactggat
     1021 tatccggaac tgctcaccca ttccggtccg tcgatccttt cccccggcaa tcattacata
     1081 aacagataaa aagcctgggt cagcgccgta tacgcttcgg aatagttctg gtctggccca
     1141 cgaatgacta agcgatcgct aaagcattct cccgcctgcg gggagaatgc cagcagcacc
     1201 cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc
     1261 atgcttaatg ccagctccgt aaaaccatta ccaatctgct ctggcagcac tacgcagaaa
     1321 aatccctctt cggtaatgca ctccgccgca caggtcagca gcgatgggtg atcaagcgta
     1381 gtggtatagc gagcctgttc ccgttgaggg atcgcgcact ctactccctg ctgatagtaa
     1441 ggtgggttac tgatgattaa atcgaagcgt actgtctgct gtgtgagcca ctgctgaata
     1501 tccgccgtat ggacgttaat ccgctctgcc cacggggact ggttgatatt ttcctgcgcc
     1561 tgcgcggcag cttcactttc cagctcaacg gcatcaatta tcacgctgtc actggttcgc
     1621 tgcgccagca tcagtgccag caacccgcta cccgcgccga tatcaaggca acgtttcacc
     1681 ccagccaccg gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacag
     1741 cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta
     1801 gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa
     1861 tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca
     1921 gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga
     1981 aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc
     2041 cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg
     2101 taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cgcgtaagaa
     2161 atccggtccg ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc
     2221 cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca ttaccggcgg
     2281 cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac
     2341 gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac
     2401 gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca
     2461 tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa cgctggaagg
     2521 cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg tttctgccaa
     2581 tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgacctgga
     2641 acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt
     2701 gtttgtgcgt aagcgtgaac gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat
     2761 caacaactgc tatctcgaag gtgagatggt acagggcaag cgtaatgaag cgattaaacg
     2821 cctgaccgaa ggccgcgtaa acgtactggt agcaaccgat gttgccgcgc gcggtatcga
     2881 cattccagac gtcagccacg tctttaactt cgatatgccg cgcagtggtg atacttattt
     2941 gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcgattt cgctggtgga
     3001 agcccatgac catctgctgc ttggcaaagt tggccgttat attgaagagc cgataaaagc
     3061 tcgcgtcatt gaggaactac gcccgaaaac gcgtacgcca agcgagaagc agaccggcaa
     3121 gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa
     3181 gccgcgggtg aaaaaacgcc atcgcgacac caaaaatatt ggtaagcgcc gtaaaccgag
     3241 cggaacgggc gtgccaccgc aaacgacaga agagtaatct taattccggg tttaagcccg
     3301 gaattaaaag tgctggcaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacat
     3361 ggctcatgca atatattgaa tttgcacgat cttgtaggcc ggataaggcg ttcacgccgc
     3421 atccggcatg aacaaaacgc acttcgtcga caagttgggt gccgggttta agcccggcat
     3481 tatttttatc ccggcaaaaa acactcttca atacactgaa taaaagtgtg catcgccggg
     3541 ctaaccgctt ttccagcatg gtgtgcgcac atagccgtaa tggtctgcga ctgttcgcca
     3601 aaaggcaatt caattaattc tccgctctca agctcttttt ccaccgcaaa gcggggcaag
     3661 taactgacac cgatattcgc cgcgacacaa cgtttgatac tttcaatgct taacagctca
     3721 atggtgtttt ccaccgtgat ccgccgctga cgcagcgtgc tctcaaatat ctgccggaag
     3781 acacattgcg gttcgttgat aataaagcta caggcgttat gtcttcccgg ctcagtaaaa
     3841 tcgacatctg caatttgcgg tgaagccacc agcaccagcg attgttcacc caactctcgt
     3901 cggttcaggg catcatcatt ccctacgcga taaaagacgc cgacatcggc ctcatcattc
     3961 agtagcgcat cacggatcac gtaacagt
//
LOCUS       DAJVTY010000235         3845 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVTY010000235 DAJVTY010000000
VERSION     DAJVTY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.236"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..713
                     /gene="pgrR"
                     /locus_tag="OQQ66_003912"
     CDS             <1..713
                     /gene="pgrR"
                     /locus_tag="OQQ66_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1906643.1"
                     /translation="GEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVEHAAKT
                     ILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRIGPDIP
                     MAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRMEGQL
                     LLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRH
                     AGSAFSLFIDRLKYKGAV"
     gene            1050..2663
                     /gene="mppA"
                     /locus_tag="OQQ66_003913"
     CDS             1050..2663
                     /gene="mppA"
                     /locus_tag="OQQ66_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1906644.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2714..3745)
                     /gene="ynaI"
                     /locus_tag="OQQ66_003914"
     CDS             complement(2714..3745)
                     /gene="ynaI"
                     /locus_tag="OQQ66_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1906645.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1082 a          848 c          891 g         1024 t
ORIGIN      
        1 cgggcgagca tcttttgtct gttcttggcc ctatgttgca tgacatagat tcagccctgg
       61 catccctgag cgagctgcag aaccgcccat ccgggacaat acgtattact actgtagaac
      121 atgcagcaaa aacgatattg ttaccagcaa tgcgcacatt cctgaaatcg catcctgaaa
      181 ttgatattca gctcaccatt gattatggtt tgaccgatgt cgtttctgaa cgttttgatg
      241 caggcgtccg tctgggtggg gagatggata aagatatgat cgccattcga atcgggccag
      301 atataccaat ggctattgtt ggctcaccgg attatttttc tcgccgaagt gttccaacgt
      361 cagtgtcaca attaatagat catcaggcaa ttaatttgta tcttcccaca tcgggtacag
      421 caaatcgctg gagattaata cgcggtggac gtgaagttcg tgttcgcatg gaaggtcagc
      481 ttttactgaa tacgatagac ctgatcattg atgctgcaat tgatgggcat ggattggcgt
      541 atctacctta tgatcaggtt gagcgggcta ttaaagaaaa aaaactgata cgtgttttgg
      601 ataaattcac accagattta cccggttatc acctgtacta tccacaccgt cgacatgctg
      661 gctcggcatt ctcattattt atagataggc tgaagtataa aggtgctgtt tagcactact
      721 tgctgataca ttaatttaat ttctctctta acgtattctc agttcctttc aacgttttgg
      781 ttatttttta ttcttcgtac aatggcgaca gatgctgatt ttgataccga aaactggttt
      841 gaacgtgcga agcctgaacg tagtattcgg tgttctatgt gctttgccgc attttggcgt
      901 cgattattta cactaaatct gatttaatat attgatacat aaaacatttt atgcttcctg
      961 tgtcactttt ttgatggaag ttgtttgcat ttctttaagg cgaaacaaat aattacgcat
     1021 caattttaat gtcggttaga gggaaactta tgaagcactc tgtttcagtc acgtgttgtg
     1081 cgctgttggt cagcagcatt tctctttcgt atgctgcaga agttccgagc ggcacagtac
     1141 tggcagagaa gcaggagctg gtgcgccaca ttaaagatga gcctgcgtcg ctggatcccg
     1201 ctaaagccgt gggcctgcca gagattcagg tcattcgcga tctgtttgaa ggactggtga
     1261 atcagaacga gaaaggggag attgtccccg gcgttgcgac tcagtggaaa agtaatgaca
     1321 accgtatctg gacttttacc ctgcgcgata acgcaaaatg ggcggatggc acaccggtaa
     1381 cggcgcaaga ttttgtctac agctggcagc gtctggtgga cccgaaaacg ctatcgccat
     1441 ttgcatggtt tgccgcgctg gcgggaatca acaacgcaca ggcgattatt gatggtaaag
     1501 ctacgcctga ccagcttggc gtcaccgcag ttgatgccca taccttgaaa attcagcttg
     1561 ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt tgccttcttc ccggtgcaaa
     1621 aagccaacgt agaaagcggt aaagagtgga cgaaacccgg aaatctgatc ggcaatggcg
     1681 cttatgttct taaagaacgc gtagtcaatg aaaaactggt cgtggtaccg aatacccatt
     1741 attgggataa cgccaaaacg gtactgcaaa aagtgacctt cctgccaatt aatcaggaat
     1801 ccgcagccac taagcgttac ctcgcggggg atattgatat caccgaatcc ttcccgaaaa
     1861 atatgtatca gaagctgttg aaggatattc cggggcaggt ttatacgccg ccgcagctcg
     1921 ggacctatta ttatgcgttt aacacgcaaa aagggccgac ggcagatcag cgcgttcgcc
     1981 tggcattaag tatgacgata gatcgccgcc tgatgaccga aaaagtgttg ggaacgggcg
     2041 aaaagccagc gtggcatttt acaccagatg ttaccgccgg atttacgccg gaaccttcgc
     2101 cgtttgaaca aatgagtcag gaagaactga atgcgcaggc aaaaactttg ttgagcgcag
     2161 ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt gtataacact tcagaaaacc
     2221 atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa gaaccttggc gtagatgtta
     2281 aattgcaaaa tcaggaatgg aaaacctata tcgatagccg taacaccggc aattttgatg
     2341 ttatccgcgc ctcatgggtg ggggattata atgaaccctc cactttcctg acattattaa
     2401 cgtcaacgca ttcaggaaat atttcacgct ttaacaatcc ggcatacgac aaagttctgg
     2461 cccaggcatc gacggaaaat accgttaaag cgcgtaatgc cgattacaac gcggcagaaa
     2521 aaatcctcat ggagcaagca ccgattgcac caatttatca atataccaat ggacgattaa
     2581 tcaagccgtg gctgaaaggt tatcccatta ataacccgga agatgtggcg tacagccgga
     2641 ttatgtatat tgtgaagcat tgatgtgatg ggaactggcg ttacccttgt ggataacgcc
     2701 agtgatgtct gatttagcgc ccctgttccg gcggtgtaat gttatccata tacagcgtct
     2761 ggctggggaa ggcaaagtcc gcgccgtgtg actgcacaat atcgataatc ttcaaataaa
     2821 cgtcttgttg tgcagcaagc cattcagccc ataccgtggt tttggtaaag caataaacca
     2881 taatattcaa tgacgagtcg gcaaactggt tgaaataaac cagtaaggtt tgtcgctggt
     2941 cgatggccgg gtgatttttc agcatctcac gtacggcttc gacaataacg cccacttttg
     3001 ccgcatcctc ataacgtaaa ccaatggtcg tggtaatgcg gcggttggtc attcgtcctg
     3061 ggttttctac gctgatcgac gaaaacagcg agttcggtac gtacaatgga cgattatcaa
     3121 aggtcgtaat tttggtaatt cgccagccaa tttccgctac tgtaccttcg atatttctgt
     3181 ccggtgaacg gatccagtcg ccaatactga aaggacggtc gaaatagagc atgatcccgg
     3241 aaaagaaatt actcagaata tctttaccgg ccataccgac agccagacca ccaataccac
     3301 caaaggtcag caagccagaa aggctcatgc cgaaatgttc gccataaagc agaacaagca
     3361 ccacaataat ggtgattttg ataatgcgcg acataatccg cgcactggtg atatcgcgac
     3421 cttttttaat ctgctgtttt tcaaactgat taatcagcaa aaatagctta atcgtcagaa
     3481 taaccgcaat cagggacgta cagataaaat cgataacgcc tggggtgata aatttgagtt
     3541 tatagttttc tataacataa ttaataatgc taccaacagc actgataatt atggtgtaga
     3601 ttaaaaattg caccgcatgg aataaaaaac cttttctttt acgatttccg cggcgaaacc
     3661 aaaagctcat cagaatcaat gctgcgcagc taccgaaaat aatgaccaga ttaagcgcat
     3721 tatttgtaaa cagttcagcg atcattgttt tatcaggctc ctccagataa ttgtcgtcat
     3781 gccggaaccc cctggcgggg ctattttacc gcgacaattc attcagatca tcaatagtca
     3841 gggaa
//
LOCUS       DAJVTY010000236         3830 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVTY010000236 DAJVTY010000000
VERSION     DAJVTY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.237"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            36..2198
                     /gene="ybiO"
                     /locus_tag="OQQ66_003915"
     CDS             36..2198
                     /gene="ybiO"
                     /locus_tag="OQQ66_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS1906646.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2258..3184)
                     /gene="rlmF"
                     /locus_tag="OQQ66_003916"
     CDS             complement(2258..3184)
                     /gene="rlmF"
                     /locus_tag="OQQ66_003916"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1906647.1"
                     /translation="MSAQKLGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3460..3719
                     /locus_tag="OQQ66_003917"
                     /pseudo
     CDS             3460..3719
                     /locus_tag="OQQ66_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
BASE COUNT          866 a         1006 c          975 g          983 t
ORIGIN      
        1 ttatcttttt gctctgtttt ctcactggag gagtcatgcg gtggatcctg ttcatcctct
       61 tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca accacaacga
      121 caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa aaagcggcct
      181 atggcgcact ggcggatgtg ctggataacg acacctcgcg taaagaattg atcgaccagt
      241 tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc gaagatcgtg ccgccgacgc
      301 tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt cagccgccat tatggtgaag
      361 ccctttccgc ccgcttcggg caactttatc gcaatattac cggctccccg cataagccgt
      421 ttaatccaca aacattcagc aatgcgctga cccatttttc aatgttagcg gtattagtgt
      481 ttggttttta ctggctgatt cgcctgtgcg cactgccgct gtatcgcaaa atgggcctgt
      541 gggcgcggca aaaaaatcgt gagcgcagta actggttgca gcttccggcg atgattatcg
      601 gggcgtttat tatcgaccta ctgttactgg cactgacatt atttgtcggc caggtattaa
      661 gcgacaacct gaatgcaggc agtcgcacca tcgctttcca acaaagtttg tttctcaacg
      721 cctttgccct gattgaattt ttcaaagctg tactacgcct gattttttgc ccaaacgtgg
      781 ctgaactgcg cccgttcacg attcatgacg agaccgcccg ttactggagt cgtcgcctga
      841 gctggttaag cagtttgata ggttatggcc tgattgtggc cgtgccgatt atctctaatc
      901 aggtgaatgt acagataggt gcgctggcga acgtcatcat tatgctgtgc atgaccgtct
      961 gggcgttgta cctgatcttt cgcaataaaa aagagattac ccaacatttg ctcaactttg
     1021 cggagcattc gctggccttt ttcagcctgt ttatccgcgc ctttgcgctg gtgtggcact
     1081 ggctggcaag cgcctatttt atcgtgctgt ttttcttttc gttgttcgat ccgggcaaca
     1141 gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc gattattggt atcgcagcgt
     1201 ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat caccctctcg ccacatactc
     1261 agcgtaacta tccggagttg caaaaacggc tgaatggctg gctttcggcg gcgctgaaaa
     1321 cggcgcgtat tctgaccgtc tgcgtggcgg taatgttgct gttgagcgca tggggattgt
     1381 tcgatttctg gaactggctg caaaacggcg cggggcagaa aaccgtagat atcctgatcc
     1441 gtatcgcact tattcttttt ttctcggcgg ttggctggac ggtgctcgcc agtttgatcg
     1501 aaaaccggct ggcttcggat attcatggcc gcccgctacc cagcgcccgt acgcgtaccc
     1561 tgctgacgct gtttcgtaac gcgctggcgg tgattatcag taccatcacc atcatgattg
     1621 tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc aggtgccggg gcattaggtc
     1681 tggctatctc gtttggttcg caaacgctgg tgaaagatat cattaccggg gtgtttattc
     1741 agtttgaaaa cggcatgaac actggagatt tggtgactat cgggccgttg accggcacag
     1801 tggaacggat gtcgattcgc tccgtgggcg tgcgccagga taccggggcg tatcacatca
     1861 ttccgtggtc ttcgataacc acctttgcta acttcgtccg cggtattggt tcggtagtgg
     1921 caaactacga tgttgatcgc catgaagatg ctgataaagc caatcaggca ctgaaagatg
     1981 cggtagcgga attgatggaa aacgaagaaa ttcgcgggct gattattggt gaaccgaatt
     2041 ttgccgggat tgtcggctta agcaataccg cgtttacact gcgtgtttcg ttcaccacgc
     2101 tgccactcaa acagtggacg gtacgctttg ccctcgacag ccaggtgaaa aaacatttcg
     2161 acctggtggg cgttcgcgcg ccagtgcaga cttattaggt gctgcctact ccgggcgcga
     2221 ccccggctga accgttaccg ccgggggagc caacgcttta acgctggcga ttgacaaaac
     2281 ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa gcgactctgc ttttgcccct
     2341 gggccatctc tttttttacc accttcaccg cgcccacgtc ggtcagagca cgatacaacg
     2401 gcggtaagtt ttcaccacga gataccagcg aggtaaacca catcacctgc ttcgcgaagc
     2461 ctttgctctc ttcaatcatc tttttgataa aggcgacttc accaccttca caccacaact
     2521 cctgttgctg accaccaaag ttcagtgcat cgtctttgtt cagccccagg ttacggcgtt
     2581 tacgctcact acctgcccgc gctgcagcgg cggaatcgtg gaatggcggg ttacacaagg
     2641 tcgcgtcgta ttgctcgttt ttatggatga tgccgttaaa aattgcccca ctctcttttt
     2701 gccgacgcag acgaatggcg cggttaagcc ccggattagc actgataatc gcctgcgcac
     2761 tgcttaacgc ctggctgctg gtttcactac cggtaaaacg ccagccatat tcatgtacgc
     2821 caatcagcgg ataaatacag ttcgcaccaa cgccgatatc cagaatgctg gcattcgctg
     2881 gaattgttcc gcttgcttca gccagtaaat cggcaaggtg atgaatataa tccgcccggc
     2941 cgggtactgg tgggcagaga aaaccgtcgg ggatatccca gttcgctacg gcgtaaaaat
     3001 gggccagcaa cgccttattg agcgccttca ccgccagcgg attggcaaag tctacgcttt
     3061 gctccccggc gggtgtaagc gtgaggaatt gcctgagttc aggattgacc tgacaaagcg
     3121 tggcgagatc gtagcggctg tgatgacggt tgcgcggatg caaccccagt ttctgggcgg
     3181 acatagcatt ctcctttaag cagcggcgta agatacccgc ataacgcgac agggtaaata
     3241 tctggctgcg tttatcattc cccaaaacct taacatggct atggtttatg gcaattcatc
     3301 ataaaaatca gcattatgca tgtcattgat ttgtcttatt ttgtaaaaat atatcgttca
     3361 aaaaataagc acatctctgt caattttttg cgtcatttgc atcgcgcagg cgacctacac
     3421 ttatcaggca ctacccgttg tatttatgag gatgtcatta tgaaaaagtg cctcacacta
     3481 ctgattgcca ccgtcctgag cagaatctct ctcacggctt atgccgcgca accgatgagt
     3541 aacctggaca gcggtcagct acggcccgcg gtaccgtttc ggcaactggc gcatcaaacc
     3601 taagcgatct ggaggataaa ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg
     3661 tcattaattc cgcaggcgga aatgaccaga tgttcggtac tgcaaccatc tacaaataac
     3721 cccaccaacg taactgcatt cgcctctgga tgcagttttc gcctgttttg taatcaaaaa
     3781 acatcaaata tgaactcaat gtaaataaat gtatttcttt ttcgcgcaat
//
LOCUS       DAJVTY010000237         3738 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVTY010000237 DAJVTY010000000
VERSION     DAJVTY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.238"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..861)
                     /locus_tag="OQQ66_003918"
     CDS             complement(<1..861)
                     /locus_tag="OQQ66_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1906648.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEI"
     gene            complement(886..1764)
                     /gene="yihU"
                     /locus_tag="OQQ66_003919"
     CDS             complement(886..1764)
                     /gene="yihU"
                     /locus_tag="OQQ66_003919"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1906649.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1937..2833
                     /locus_tag="OQQ66_003920"
     CDS             1937..2833
                     /locus_tag="OQQ66_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1906650.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2873..3661
                     /locus_tag="OQQ66_003921"
     CDS             2873..3661
                     /locus_tag="OQQ66_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1906651.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          963 a          955 c          902 g          918 t
ORIGIN      
        1 aatttctgcc aaccgttgta aacgaggaac agagacatcg cgcagcatag tttgtggatc
       61 ttgtgcacca acaaccgacg cccaaaccgc tcgtccagcc aggaagcccg acgcaccacc
      121 tttcatggca atacttactg cacggccaaa aatatcggca tcaacgccag aagaaagaat
      181 tacccacggc atcttcatct gatcgttaag ttgctgacag gcggcaagta aggttttttc
      241 atcgcctttg ccccctaatg gcatctcggc tttgtaaaga tcagcctctg ttccaccaag
      301 ctctgcggcg gcggcaacaa tggcgctttc acgatcaaag tcccaaccgc gacgaggtgg
      361 acgcaccaca ggttcaataa tgctcaccag cccggcacta cggcagcgac gaacaaattt
      421 atcaaccatt gccagacgtt cctctgcggg ttcatcttca cgccagagta caagtaattt
      481 cattgcctta gcccccattt cacgggcttt atgcgggtcg acggacatat ctatttcaac
      541 gctatcaacc gggatgccat tacctgggat aaaaagatcg gcagcgacaa tcaggccgca
      601 agaattagca acagcgccct gctcgactat ttgctcaaga caaaattgtt tatccgccag
      661 aacggcagaa gcataaggag aaaggattcg ggtcgcggca actttaaaat cggtgagtac
      721 actgtcggcg atgggttttg gctgccctgc tgccgcaaac atcaagcgca tggcctcacg
      781 ctgatcgaca gctaacatgg caaatccacc acctgggcgg gagatatctt tcagcgtaaa
      841 tggggtgaag tgggatgaca tagaaactcc tgttaataga taaaattaaa gaccagcctg
      901 ctggcgaacc tgatttagca gggcactcca gtcctgccgc cctttgccgt cttgacttgc
      961 tgcagcgtaa taactttctg ctgcaatccc catattgagc ggtgtccccg tctcccgagc
     1021 gacgtcaact gcaatacgca gatctttcaa cgccagatcc accatgaagg cgggtgaaag
     1081 atcgcctttc agcactttgc caggccaggt ggtcgtaaaa tgtcctttcc ctgctgccgt
     1141 accactcatc accttaattg cgacatcaag atttaatccc agtgactcac ataacacagc
     1201 ggcctcggag gacagcgcat taagcgcaat gctcatatag ttgttaatta gcttcacgcg
     1261 gatccccatt cctgggccgc cagcctcgac cagctcatta cccatgcaca taagaatcgg
     1321 gcgtgcacgt gcaatctgct catcagtgcc acccgctaaa atcagcaatg tgccatcaat
     1381 cgcattcacg gacgttctgc ccacgggagc gtccatcata ctgatgcctt tttcttgtaa
     1441 ctcccgtatt aatgcatcgg tctgtaaggg atgaatagtc gacatatcaa tcaataacgc
     1501 gtcagatgaa acagcctcac agacgccttt ttcccccaac agaacctgtc gaacaatgtc
     1561 accattggga agcatagtga tgataaattc agcgcctgct gctgcttccg cttgggtttg
     1621 tgccgcagtg gccccctgcg taactagcgc atcgacggcc tgtgcattga catcaaatac
     1681 ctgcaatgaa tgcccttttt gcaatagatt gttagccatt gggctaccca tctgccccag
     1741 cccgagaaat gcaatggttg ccatgaatac tctcctgtct gacattgaca aaataatgac
     1801 aattgaaaat tcgtttttgt ctgtttttga gcatatttgt gatttatgtt caaaaacacg
     1861 acacgcatca ctaatttgat catttgcgtc atttaaattg accaaagacg acaaaattaa
     1921 acaggacaag acaaatatga ccagaattgc atgcgtgggt atcacagttc aggacagaat
     1981 ttattctttg cccactctgc cggagggcgg cgggaaatat caggcaaatc attatcttga
     2041 gattggtggc ggcccggcag caacagccgc tgttgcaatc gcaaagcttg gcgtggaggt
     2101 tgatttcatc ggtcgcgtcg gcgatgacag ttgtggaaat accttacttg cagaacttga
     2161 gggttggggc gtcaataccg cattttgccg ccgatatcct aacgcccgtt cgtcgcaatc
     2221 agcgatcctc gttgatcagc atggcgaacg gattatcgtt aattatccaa gtcctgatct
     2281 cggtactgat gccgaatggt tagaagcgat cgatttctct cgttataatc tgattctggc
     2341 cgatgttcgc tggcatagcg gtactgaaaa agcattctcg ctagcgcgcc ttgcgggtgt
     2401 gacaacgctt ttagacgccg atatgacacc gcaggatatt tctccacttg tcgcgcttgc
     2461 cgatcacgcc gtgttctcaa caccagggct gaaacgcatg actggcctgc agtctcctga
     2521 agaaggtttg ttccaggcaa cgacacaaac agcaggtaaa gtgtatgtta cgctgggttc
     2581 tgaaggatcg ttgtggattg aagatggtca tttgtgtcaa caggaggcat tttctgtcaa
     2641 tgtggttgat accactggag caggcgatgt ttttcacggc gcactcgcgg tagcgttggc
     2701 agaaaaaatg ccaacaaaag aggctattcg ttttgccagt gcggttgccg cgatgaaatg
     2761 tacccagcct ggcggtcggg ccggcattcc taatcgtgag caaaccgaat catttttgtc
     2821 actttatgcg taaaatggta aacatctacc gaggaggggc aagggagtat tcatgagtat
     2881 catcgaagtg acaggtaatc cacgccatga ccaactcgtt catctaatcg cagaacgcgg
     2941 ttatatgaat attgaagaac tggcacaact gctggatgtt tcaacgcaaa ccgtgcgccg
     3001 ggatatccga aaactcagtg agcagggctt gatcacccgt catcatggag gggccgggcg
     3061 cgtctccagc gtcatgaata ccgcttttga gcaacgggaa ctttcgctca ccgccgagaa
     3121 acgggcgatc gctgaggcag tcgccgatta ccttcccgaa cgctgtaccg tctttatcac
     3181 catcggtaca accgtagaag ccgttgccag ggcattactc aaccggcgtg atttacgcat
     3241 tatcactaac agcctacgtg tggcacagat tctttataag aatcaggata ttgaagtgat
     3301 ggtgccggga ggcactttac gcgctcataa cggcgggatt atcggcccag gagccgtgga
     3361 ttttattgaa ggcttccgcg cagattattt aatcaccagt ataggtgcca ttgaacatga
     3421 cggcacccta ctggaatttg atctaaatga agcgttagtc gcgagaacga tgattaaaca
     3481 tgcgcggaat acattgttag tggccgatca tacgaagttt gccgcgtctg ctgccgtttc
     3541 aattggcaat gcacggaatg tcagggcttt ttttactgat gccccgcctc ccaattcttt
     3601 ctgccagttg ttaagtgaag agaatgttga actggtggtt gccgagcaag aagtatcctg
     3661 aaattgaaat ttcggaaaat ccgctgggaa tttcagcgga ttttgttttc cataagcaaa
     3721 aaaaacctgc cagcgatg
//
LOCUS       DAJVTY010000238         3724 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVTY010000238 DAJVTY010000000
VERSION     DAJVTY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.239"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            37..264
                     /locus_tag="OQQ66_003922"
     CDS             37..264
                     /locus_tag="OQQ66_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1906652.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="OQQ66_003923"
     CDS             complement(265..639)
                     /locus_tag="OQQ66_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1906653.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OQQ66_003924"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OQQ66_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OQQ66_003925"
     CDS             complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OQQ66_003925"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1906654.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3724
                     /locus_tag="OQQ66_003926"
                     /pseudo
     CDS             3263..3724
                     /locus_tag="OQQ66_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          952 c          945 g          912 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcgcaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 acat
//
LOCUS       DAJVTY010000239         3672 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVTY010000239 DAJVTY010000000
VERSION     DAJVTY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.240"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..201
                     /locus_tag="OQQ66_003927"
                     /pseudo
     CDS             1..201
                     /locus_tag="OQQ66_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            183..1565
                     /gene="torZ"
                     /locus_tag="OQQ66_003928"
                     /pseudo
     CDS             183..1565
                     /gene="torZ"
                     /locus_tag="OQQ66_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(1730..2701)
                     /gene="cmoB"
                     /locus_tag="OQQ66_003929"
     CDS             complement(1730..2701)
                     /gene="cmoB"
                     /locus_tag="OQQ66_003929"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1906655.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2698..3441)
                     /gene="cmoA"
                     /locus_tag="OQQ66_003930"
     CDS             complement(2698..3441)
                     /gene="cmoA"
                     /locus_tag="OQQ66_003930"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1906656.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3482..>3672)
                     /locus_tag="OQQ66_003931"
     CDS             complement(3482..>3672)
                     /locus_tag="OQQ66_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1906657.1"
                     /translation="ETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLL
                     MVLANLWYMPWELVFSLR"
BASE COUNT          966 a          908 c          978 g          820 t
ORIGIN      
        1 gctaccgtga atcctggtac cgatctcacc gtcgttgatg ccagtggcaa acagccgatc
       61 gttctgttgc agggttatca aatgcagggc agtgaaaaca cgctctacct ggcggcaggt
      121 caacggctgg cgctagccac attaagtgaa gaaggtatca aggcgctcac gctaaacggg
      181 gaatggcagg ctggggaatt cagcgccagc aatatggtga acagaaacac tggatgctgg
      241 tgacgctggc ggcggtgtta gggcagattg gtacaccggg cggcggtttt ggtttctctt
      301 atcattactc caatggtggc aacccgacac gtgttggcgg cgtgctgccg gaaatgtctg
      361 cggcgattgc cggacaggcc agcgaggccg ctgatgatgg cggaatgagg gcgattcctg
      421 ttgcccgtat cgtcgatgcg ctggaaaatc ctggcggcaa atatcagcat aacggtaaag
      481 agcaaacgta tccgaatatc aaaatgatct ggtgggcggg tggcggtaac tttacccatc
      541 accaggatac taatcgtctg attaaagtat ggcagaaacc ggagatgatc gtcgtttctg
      601 aatgctactg gaccgcagct gctaaacatg cagatatcgt attaccgatc accacatcgt
      661 ttgagcgcaa tgacttgacg atgaccggtg attacagcaa ccagcatatt gtgccgatga
      721 agcaggctgt cgctccgcaa tttgaagcgc gaaacgattt tgatgtgttt gccgatcttg
      781 cggaattact gaaacctggc ggaaaagaga tctataccga aggtaaagat gaaatggcgt
      841 ggctgaaatt tttctatgat gccgctcaga aaggtgcccg tgcgcaacgc gtgactatgc
      901 cgatgtttaa tgccttctgg cagcaaaata aactgatcga aatgcgccgc agcgagaaga
      961 acgaacagta cgttcgttat gctgatttcc gcgccgatcc ggtgaaaaat gcgctgggta
     1021 cgccaagcgg caaaattgag atttactcca aaacgctgga aaaatttggc tataaggatt
     1081 gcccggcaca tccaacctgg cttgcgcctg atgagtggaa gggtaccgcc gacgagaagc
     1141 agttgcagct tctgaccgca catccggcac accgtttaca tagtcagctt aactatgcgg
     1201 aactgcgtaa aaaatatgcg gttgcagatc gtgaaccaat cactattcat accgaagatg
     1261 ctgctcgctt tggtattgcg aatggtgatc tggtgcgtgt gtggaacaaa cgtggtcaga
     1321 ttctgacagg cgcggtggtg actgacggga tcaaaaaagg cgtggtatgc gtgcatgaag
     1381 gtgcatggcc agatctggaa aatggcttgt gtaaaaacgg cagtgcgaac gtgttaacgg
     1441 cggatatccc cagctcgcag ctggcaaatg cctgtgccgg taactctgcg ctggtgtata
     1501 tcgaaaaata tacgggtaac gcaccgaagt taacggcatt tgataagcct gctgttcagg
     1561 cgtaagaagt gaatagcgcc cggaaacggg cgctgaggtt atgcaaacgt aaaactgcct
     1621 gatgcgctat gcttatcagg tctacatagt ccctgcaata gattgaatta gcaagggttt
     1681 gtaggccgga taaggcattc acgctgcatc cggcagtaat accagacctt tacggcttgc
     1741 gcgcaatcag caccgcgcgt ttaggcgcag gataaccttc aactgtctta ctcggatcat
     1801 gtgggtcgag gaaatcggac agagattcgg taaccatcca ttcagtgcgt cgctgctctt
     1861 cagtagtggt aacgcacaca tccacaacgc ggatatctac aaaaccacac ttcttcagcc
     1921 agtttttcag cgccagcgcg gaagggatga aatagacatt acgcatttgc gcgtaacgat
     1981 cgcctggtac cagcaccgtg ttctcatcgc catcaataac cagcgtttcc agcaccagtt
     2041 caccctcatt caccagttga tctttcaact gccagagatg ctccagcggc gaacggcgat
     2101 ggtaaagcac gcccatcgaa aagacggtat caaaggcttt cagtgccgga agctgttcaa
     2161 tacctaacgg taacaaatgt gcgcgctgat cgttacccag tagtttacgc accgcttcaa
     2221 actggcacag aaacagttgc gtagggtcga tccccaccgc gaggtgcgcg cctgcgccaa
     2281 tcatgcgcca catgtgataa ccgctgccgc agccgacatc cagaatggtg cgtccggtta
     2341 aatcagaaag atggggcaga acacgatccc atttccagtc ggaacgccat tcggtatcga
     2401 tgttgacgcc atacagtgag aacggccctt tgcgccatgg catcaggttc cgcataagcg
     2461 tttcaatgcg tttaatttgc ccggtgctca gtggctcttc gctttcggcg gttacgctat
     2521 gcaataaatc cagacgatac ggtttaattt caggcagaaa ttccaccgcg ttggaccact
     2581 gcttaaacag cccgtgctgc tgctcgcgct gccagttagc aatctgcgcg ggcagcgttt
     2641 cgagccagtg tgaaagatga tttttggcaa tcagagaata aaagttacca aagtcgatca
     2701 tgcagcgtcc tctgctttta atgccaccag tgaaccaaag ttaaagcact ggaaccacag
     2761 ctcgctatgc tcaaaaccgg ctttatgcag gcgtgcttta tgggtttcca cggaatcggt
     2821 cagcatcacg ttttccagca tgctgcgctt ctggctgatc tccagttcgc tgtaaccgtt
     2881 ggcacgttta aagtcgtggt gcatgttgaa caacagttca ccaactttgg catcttcgaa
     2941 actgaatttt tccgaaagca ccagcgcgcc gcccgggttc agtccttgat aaattttatc
     3001 cagtaatgcc tggcgctcgg aaggttccag gaattgcagg gtaaaattca gcaccaccat
     3061 cgatgcgttt tcaatggcga tatcgcgaat atcaccttca atgacgtcta ctggcgtagg
     3121 ggctttatag gcgtcaatat gacgacggca gcgttcaatc atcgccgggg agttgtcgat
     3181 ggcaataatt ttgcaattat catgatgaat gttgcgacgc accgagagcg tcgccgcgcc
     3241 cagagaacaa cccagatcgt aaacctgcgt accaggttga acgaagcgct cggctaacat
     3301 actaatcatg gaaataatat tggaatagcc gggaacggaa cgctggatca tatccgggaa
     3361 gacttcagct acccgttcat caaaggtcca gtcgcccagt ctggcgatag gggcagaaaa
     3421 tagcgtgtcg cggtgagaca taacgtaaaa atccgggaaa aagaaagtgg cgtattgtgc
     3481 gctaacgcag ggagaaaacc aactcccagg gcatatacca aagattcgcc agcaccatca
     3541 gcaacagcgc acaccaggtg gcgctcatgc cggaacgtcg ccagcgaaac agacggtgat
     3601 gaaaaccgta ataatgcatc agacgaccag caagcaaaac gatgccgcaa atatgcacca
     3661 tccaggtttc tg
//
LOCUS       DAJVTY010000240         3651 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVTY010000240 DAJVTY010000000
VERSION     DAJVTY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.241"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..93
                     /locus_tag="OQQ66_003932"
     CDS             <1..93
                     /locus_tag="OQQ66_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCS1906658.1"
                     /translation="FWCRQIDKRAAEKISASQSAGSKPEEPVTP"
     gene            119..364
                     /locus_tag="OQQ66_003933"
     CDS             119..364
                     /locus_tag="OQQ66_003933"
                     /inference="COORDINATES: protein motif:HMM:NF017507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCS1906659.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGVAAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            441..656
                     /locus_tag="OQQ66_003934"
     CDS             441..656
                     /locus_tag="OQQ66_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1906660.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            661..1005
                     /locus_tag="OQQ66_003935"
     CDS             661..1005
                     /locus_tag="OQQ66_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCS1906661.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1056..1589
                     /locus_tag="OQQ66_003936"
     CDS             1056..1589
                     /locus_tag="OQQ66_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1906662.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1745..1927
                     /locus_tag="OQQ66_003937"
     CDS             1745..1927
                     /locus_tag="OQQ66_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906663.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2254..2406
                     /locus_tag="OQQ66_003938"
     CDS             2254..2406
                     /locus_tag="OQQ66_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCS1906664.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            2492..2716
                     /locus_tag="OQQ66_003939"
     CDS             2492..2716
                     /locus_tag="OQQ66_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906665.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3061..3387
                     /locus_tag="OQQ66_003940"
     CDS             3061..3387
                     /locus_tag="OQQ66_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCS1906666.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(3519..>3651)
                     /locus_tag="OQQ66_003941"
     CDS             complement(3519..>3651)
                     /locus_tag="OQQ66_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HCS1906667.1"
                     /translation="QINIALDLKGSGNFSAWVIEACRRRLATDATHLRPASMKNNEK"
BASE COUNT          921 a          807 c         1023 g          900 t
ORIGIN      
        1 ttctggtgca ggcagattga taaaagggcc gctgagaaaa tcagcgccag tcagtcagcc
       61 ggaagcaaac ccgaagaacc cgttactcct taacaacccc tttcagcgag aaaatcccat
      121 gtcagaaatc acatccctgg tcactgcaga ggcagtgaag gaagtcctgc gctctgaaga
      181 agtccggagc gcactgaaac agaaacttcg ccagaatctt gagtcgcgtc ttgatgcaga
      241 agtggatgcc attctggatg agctgctggg cgtagcagcg gttcctgagc cggaaggtat
      301 cgcgggtgac gggagtgctt cagatggcgg tgaacctaca cctgacagcg acatgatgat
      361 gtaagcatgc gcaagggacc atcggtgtgt gccggtggtc tttatattgt tgtgagcttc
      421 cggattgcgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt gtgtcataca
      481 ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg ttttccccgt
      541 ctcagtgggc ggcaataggc gtgctgggga gtctgttgtt tgggctgctg acatatctga
      601 ctaacctgta tttcaaaatc agagaggacc gtcgtaaggc ggcgcgggga gagtaaagcg
      661 atgaagaaaa aatacgaact ggttgttaaa gggataaata attacccgaa taagattact
      721 gttactgtgg cactggaaat tggtgggtat ccgtcactgt tgttgccaga tgtggcgatt
      781 agtcttgacc gtactgaaga tgccacgctg gagttttacg aagctgaggc gaaaaagcag
      841 gcgaagcagt ttttcatgga tgttgctgcc gggttatgtg aaggggatgg tccgttgccg
      901 gaaaagcgcc ccgtaatttt agaggcgcag gatgtgttga taacctacag aggaaaacta
      961 ccgggaataa ttactggttc tctgaagact ccaccgctgg cctgaagact taacatatcc
     1021 agggatttga aatcgataaa ccctgataaa tatccatgaa caccaaaatc aaatacggcc
     1081 tgtcggctgc cgttctggcg ctgattgccg caggtgcgcc tgcgcctgaa atcctcgacc
     1141 agtttctgga tgaaaaggaa ggtaaccaca ccacggcata ccgtgatggc gcgggtatct
     1201 ggaccatctg ccgcggtgcc atcctggtgg atagtaaacc tgtcgtcccg ggcatgaagt
     1261 tgtcgaagga aaaatgcgac caggtcaacg ccattgagcg tgataaggcg ctggcatggg
     1321 tggagcgcaa tattaaagtg ccgctgaccg aaccccagaa agcgggtatc gcgtcatttt
     1381 gtccctataa cattggcccc ggtaagtgtt tcccgtcgac gttttacaga cggattaatg
     1441 ctggtgaccg caggggagca tgcgaagcga ttcgctggtg gattaaggac ggtggcagag
     1501 actgccgtat ccgctcaaat aattgctacg gtcaggtatc ccggcgagac caggaaagtg
     1561 cgctggcgtg ctgggacatc gacagataag cagaatattt tgctgaaaat aaggcatgag
     1621 cacgcggacg gataacacga aatcctgcga actggcgaaa cgtaagtgaa taaaagtaaa
     1681 aaccccgttt gttggcacca ggcggggttt tgtgtttctg accttgagta aggcaaggga
     1741 gaacatggcg aagtataaac gaattctgtt gaggttgacc atgaaaaacg gccttgaact
     1801 gaaagcgcct gtaactgatg acatcagcag agcactggct tttgccatta agtgggtggc
     1861 ggtcggtgtt gctgtgtccc cgatgctgta tgggctggca aaactggtca ttgcgttgaa
     1921 atcgtgaagg gaggattaag catgtcagac aaactcataa cgctggcgaa gatcctctgt
     1981 gtaattgtcg gcatttcatt ttcactaatg ctggttgctc tttttctttc catggcctgg
     2041 atgatgttgt cttcgtcggg gctgctgggg tgaacataaa ccgaatgctt tccgcgttta
     2101 ccgttattct gctggtggtc tgtggtgcgc tgtggctggc aacagaccat taccgtgata
     2161 acgccatcac ctacaaagcg cagcgcgata aaaaagccag agagctggag ctggcaaacg
     2221 caaccattac tgacatgcag cagcgccagc gtgatgttgc cgctggtcgt aagcgcctgc
     2281 ggatcaacgc cacctgctcc ggtaccgtgc gtgaagccac cggcacctcc ggaatggata
     2341 atgcaaccgg cccccgactg gcagacaccg ctgaacggga ttatttcacc ctcagagagc
     2401 ggttgatgac aatgcagaag caactggaag gggcgcagga atatatccgc actcagtgta
     2461 ttaactagta tttttgttat ccggagaatg catgaagaaa ttactggtaa ccgtaaagcc
     2521 ttttcaggga acaattccgt tccgtatttt gcagcgtggt cgtgttcttg ttgaaggttc
     2581 gttcagtggt aaatgtacgc aattacactc ccggaccttt caggtgaatg ccacgaatga
     2641 agagctaacc gttgagtgta cgatgaatgc cgctaaatgc cgcatggtat ccgctgcatt
     2701 acagccagtg tgttgagcga ccttattaac catgcgcggt attgtcgccg tatccccgca
     2761 ttaacagaga ccgcagcccg accgggagac tcctctgcgc gagtgtgcgg ggataatcaa
     2821 aaacgataca caccggggtt taccgcgtta acggagcgcg gcgttgtccc ctcatggtcg
     2881 ctggtccggt gcgatggtgg aagaaaccgg acgatgtgtt acctcgcaag ctctgttatg
     2941 tcatgtgtct gatttgtgat ttaagtcgga taattgtcgt tgccattaag cagaggattg
     3001 atgaccgaca gggtggcatt gttagaataa gacttattct tatctgtgcc gggaatgaaa
     3061 atgaaaagaa accttccgtt aattattttg ttgtcttctc tggttatggg ctgtacgcaa
     3121 cataaaacag atatgccccg acagttggtt aaggcattac cacaatatcc ggcctatgca
     3181 gcggcaaatt atataaaggg acgggttgat gtgaagtttg atattggtgc tgatggtact
     3241 gtcacccgaa ttgagtttat ccgttcagag ccgcaccatt tgtttgatga gcaggttgta
     3301 aaggcgatgg caaaatggcg atttgagaag gacaggccgt gtaaaggcgt gaagaaaacg
     3361 tttatcttta gtccttctgc accctgatta tttcatcaga aattaattat cactctgttg
     3421 ttattctgta catcccggca gggtaagtct tgttccgtcg gatatgaaga tgaaatattg
     3481 ttggaggaca gtgggtacct gctcctgtaa ccgaacgttc atttctcgtt atttttcatg
     3541 ctggccgggc gcagatgcgt tgcatctgtt gccagccttc tcctgcaggc ttcaataacc
     3601 cacgctgaaa agtttcctga acctttcaga tcaagagcga tgttaatttg t
//
LOCUS       DAJVTY010000241         3620 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVTY010000241 DAJVTY010000000
VERSION     DAJVTY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.242"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(303..722)
                     /locus_tag="OQQ66_003942"
     CDS             complement(303..722)
                     /locus_tag="OQQ66_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906668.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            802..1056
                     /locus_tag="OQQ66_003943"
     CDS             802..1056
                     /locus_tag="OQQ66_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1906669.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            1053..1478
                     /locus_tag="OQQ66_003944"
     CDS             1053..1478
                     /locus_tag="OQQ66_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1906670.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1550..2620
                     /locus_tag="OQQ66_003945"
     CDS             1550..2620
                     /locus_tag="OQQ66_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS1906671.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            2661..3083
                     /locus_tag="OQQ66_003946"
     CDS             2661..3083
                     /locus_tag="OQQ66_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1906672.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            3080..3385
                     /locus_tag="OQQ66_003947"
     CDS             3080..3385
                     /locus_tag="OQQ66_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCS1906673.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            3372..>3620
                     /locus_tag="OQQ66_003948"
     CDS             3372..>3620
                     /locus_tag="OQQ66_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1906674.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLK"
BASE COUNT          966 a          790 c          983 g          881 t
ORIGIN      
        1 tccatgctga cctcatttcc ccttaacgcc ggggtagcgg aacaaaaacc tgctgcatag
       61 ttattaaagt tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac ttaacccctg
      121 accactgcct ggtaactcga agtattgccc tgcattctgt ggggcggggt gggtggcagg
      181 tatataatgt actttgcgtt cattgttgta aagtgcttat ggtacatttt gtgtgtaaaa
      241 aaatgagatg ggataaagtg aagcacaaac ccggaggaag gcgctaccgg atttatgctg
      301 gtttaagagg ctttttgttt tttctttcgt gctaactctt cgtaaattgc attgtacttc
      361 tgttttttct cttcaagagt ttttaaaagt tcatctgtct cactgtcagg gagctcgtcc
      421 agaaggtcaa tgatgatttt ttgtcttgga tttaactcct gatagaaacg tacctgtcca
      481 ctttcttctg tatcctctcc caaaagatag gttggtgttg ttcctattag tgttgctaat
      541 tcccttaatt tctcccggcg aggaattgtt tcgccattaa accatttgct aaccgctttt
      601 ggtgttaatt tcattcgacg ggcaatttct gcctgccttc catgttgttc ataaccagcg
      661 ttttcacagg ctagcgcaag cctactggcg aactctttac gcgctttatc ttcatgaacc
      721 ataagttcaa tgatattcgc tcttgaatgt actgtcagtt ctgttatagc atgtactcaa
      781 agttcacatt gtgagggtga tatgaaccag aaaacacttg aagatgtaat caaaactgtt
      841 cgcgttgctg ttgtggccga cgtttgtggt gtcagccaaa gagcaatcta taaatggatg
      901 gataacggaa aattgcctcg cacagaatat accggcgaaa caaattacgc tgaaaaaatc
      961 gctcttgcat caaacggatt attttctgcc gatgcaattt taactattgg caggaataaa
     1021 actactacga aaaagctgat gggagttgat tcatgaaaat caagcatgaa cacatccgca
     1081 tggcgatgaa tgcctgggcg catccggacg gcgaaaaagt accggctgcg aaaattacca
     1141 aagcgtattt cgagctggga atgacgttcc cggaactgta tgacgacagc catccggaag
     1201 ccctggctcg caatactcag aaaattttcc gctgggtgga gaaagacacc cctgatgcgc
     1261 ttaaaaaaat tcaggcgttg ttaccagcta tcgaaaaagc aatgccacct ctgctggtgg
     1321 cccgaatgcg cagccatagc tcagcctatt ttcgggaact agtggagacg cgggaacgac
     1381 tggtgagaga cgctgatgat tttgtcgcag tggcgatcgc tggtttcaac cagatgaatc
     1441 gtggtggccc tgcaggaaat attgtggctg tgcattgact cgcaatattc ataccggatc
     1501 acttccggca atttgtgagt aaaaagattc ggtatcaaaa gaggtgagta tggctaacgc
     1561 ctggctcaga ttatggcatg acatgccaaa tgaccctaag tggcgaacaa ttgccagggt
     1621 gtcagggcag ccaattgcaa cagtgatggc agtgtatatc cacctcctgg tgagcgcgtc
     1681 acgaaatgtc acgcgaggtc acattgatgt cacgacagaa gatttggcaa gtgcgctcga
     1741 cgtgacagaa gaggtaattg attcaatttt gcagacgatg caggggcggg tacttgatgg
     1801 tgatttaatc actggatggg aaaaacgcca ggtgctgaaa gaggacaacg gcaatatttc
     1861 gcaaaccgca aaatctcctg cagagcgcaa gagggcgcag cgagagaggg aaagaaagcg
     1921 ggaacaaaat ggcgattgtc acggcgcgtc acgatatgtc acgcacatgt cacgacgagt
     1981 cacgacagat aaagatacag ataaagatac agatcaagaa gatcaaaaca ctatggtcca
     2041 tggcgtaaaa aacgccacga accaggcagg ggatgttcag accgtcaatc ctggtcagcc
     2101 agcaggcacg acaccggaag ccgattcagc gtatgcgctg aaagccgatt cgggcgctgt
     2161 gcagcaggcg atgaccgcaa gtccggagca atcacaccaa ctgcagcagc ctgaagccga
     2221 ttccgccatt cagcgggaag ccgatcgggt agtcccggaa aacaccgggc agtctgtggg
     2281 acgagtggat tatccggatg tgttcgaaca ggtctggcgg gagtacccgt tgcgtgccgg
     2341 ggcaaacccg aagaaatccg ctttcagtgc ctggaaggcc agattacgcg agggggtgcc
     2401 accagaggcc atgctggatg gcgtgaggcg ttacgcaaga tacctggcgg ctaccgggaa
     2461 aacgggaacg gaatttgttc agcgagcgac gacgtttttt ggaccggacc ggaattttga
     2521 gaacccctgg ttgctcccgg taagcggcac gaacaaccag cgttgtgtga atcatatttc
     2581 tgaaccggat aacgaaattc cgccgggctt cagggggtaa gtgttaattt ctggtcatga
     2641 ggtaattttc aggagggctt gtggcaaaag tttttacaca agaagagcgg gaaaaaatta
     2701 aagggcaggt tcttgaactc gtacgccaga gtgggcgcga gacgttacga caactggaag
     2761 ctaaaactgg ggcaacaaga tatctgatga gcgttctggc cagagagctg gttgccagtg
     2821 gcgatgtata caactctggt tacgggttat tcccgtctga acaggcgcgt aaggactggc
     2881 aaaatgcccg taaaaagctc tcaagggcaa agctgaagaa accatctgcg gttgatccgg
     2941 accttatctg gtcattacct gacggagaaa tacgtcgcta cgacagtcgc ctaaacataa
     3001 tctgtcgcga gtgccggaag agcgaagtta tgcagcgcat attgtcgttt tatcagggta
     3061 attttcagga ggtgatggcg tgagggtgag agtctatatc gccggtccaa tgaccgggta
     3121 taaaaatttc aaccgtgagg cgtttcacaa tgcggaagag gaactgaaac gggaagggca
     3181 taccgtctta aacccggcag tacttccgga cgggctgaca cagccgcagt acatggatat
     3241 ctgcatggcg atgattcgtt gtgtggatgc gatttacatg ctgaaaggct ggcagcggtc
     3301 agcaggcgct aaggcagaac tggcgctggc ggagaaactg gggcatgcgg tgatttttca
     3361 ggagacagcc agtgtgcaag actaattacc aggcattacg tgaacgttat tcaccaattc
     3421 aggtgccgga atgtcctgtt tgtggcgatg aaatgtcgat acagcgcata ttttccagaa
     3481 cgcatattgt gtatgcctgc acaggtgagg gggatgatgg atattttaaa actggtcgaa
     3541 cttttgcgga tgagcattac ctgaaatcgc gcgtaaccgt cgttgatgtt agcgctcctg
     3601 acgtactggc tttactgaaa
//
LOCUS       DAJVTY010000242         3581 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVTY010000242 DAJVTY010000000
VERSION     DAJVTY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.243"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(175..462)
                     /locus_tag="OQQ66_003949"
     CDS             complement(175..462)
                     /locus_tag="OQQ66_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1906675.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(537..680)
                     /gene="mgrB"
                     /locus_tag="OQQ66_003950"
     CDS             complement(537..680)
                     /gene="mgrB"
                     /locus_tag="OQQ66_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1906676.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            839..1078
                     /locus_tag="OQQ66_003951"
     CDS             839..1078
                     /locus_tag="OQQ66_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906677.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1221..2012)
                     /gene="kdgR"
                     /locus_tag="OQQ66_003952"
     CDS             complement(1221..2012)
                     /gene="kdgR"
                     /locus_tag="OQQ66_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1906678.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2189..3562
                     /locus_tag="OQQ66_003953"
     CDS             2189..3562
                     /locus_tag="OQQ66_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906679.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVMLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          872 a          888 c          802 g         1019 t
ORIGIN      
        1 aattaatgag gaaaatatct ttttctgata tcccgttacg caaataaata atatgataat
       61 tatttgaagt cagaccagag ttttaatttg aagcagttgc accatgacag tgcgtataat
      121 caggatgaat tgagtaaccc ttcaacgacg gggttactca agattgagga aggattatcg
      181 ttcggcgacc aggcgaataa tatcttcgtc atcaaccgat ttttgactct caatggtctt
      241 tttatctgct ttctctggtt cgtttgcttc gcacaaacga cgtaacagtg cattttgacg
      301 tttttgctga tccagcaacg cctcaagcag ttcaatctgt tcgttagtcc gtgaactggc
      361 acgattgata aaaaaccaca agatgagccc gataagaaga actaccaccg atacaaccaa
      421 agacgcaatg ttcatcacgc ctgaatttac aacttcgttc atttcaccac ctctgagtag
      481 aggcgctatt ctaccactgc tggagaggaa gaaaatctag tgctgaaaaa atgatatcac
      541 cacgggataa actggttaat ggcacaaatt ccgctgaaaa attgtacatc ctgatcgcac
      601 atcatgttga atacctgcgc ccaaagcagc aagcaagcca acaccacgac aaccagaacg
      661 acccatcgaa actttttcac tccaatctcc gcttcaccta ccttatgtca ttaaactagc
      721 atgcgttagt taaacagcgc ctaactatgt cgatattcgt gctttttcgg aatgagtagc
      781 ttgcttcagc gcatattgcc gctacgatta agcgaacata aaaagagaag aggttgtaat
      841 gcgattcatc attcgcacag ttatgctgat agcactggta tggataggat tattactcag
      901 cggatatggc gttttgattg gaagcaaaga gaatgccgca ggattaggtt tgcaatgtac
      961 ctatctgact gccagaggca ccagcacggt acaatacttg cacactaaaa gtggtttctt
     1021 ggggataaca gattgcccac tgttacgtaa aagcaatatc gtggtcgata atggttaagg
     1081 gtaataaata accagattct cactcatgag taaaatattc ttatagcaag cgcgtttaat
     1141 ataacgcacc ttcaacttta tttttctcat gctaaataaa gaacctgtct gtttgatatt
     1201 ccgacaggtt cttactctca tcagaacgga tagtcgtgat aacccatttg ggcagaaatt
     1261 ttgcgcgctg cggtatgcaa cattgcgaca tattcctgta aacgctcttc agagaaacgc
     1321 aacgtcggga aggagatgct caaaccggca atgaccacgc caaagcgatc aaataccggt
     1381 accgcaatgc atcgcagccc ttcttcctgc tcttcgttat cttcgccata cccctgctcg
     1441 cgcacctggt ccagaacggg taataacgct tctgtactgg tgatggtccg ctcggtactg
     1501 cgtttatact ccacgccctc aagaatttgc ttcacttcat cgcgatcgcg ccatgccagc
     1561 agtaccttac caatcgcggt gctgtacagc ggattacgac gcccaatccg tgaatacatg
     1621 cgcaaattgt acatagagtc aattttgtga atgtaaacaa tactgtcttc gtccagtgcg
     1681 ccgaggtgga tagtttcttt ggtcaggcgg gagatctcac gcatctggat atctgcgcta
     1741 cgaattaaat cgacgttttg taacgcgcga gcgcccagtt caaacaattt cagggtcagg
     1801 gaatatttct ccgactcccc ttcctgcgcc acataaccta aggttttcat ggtctgtaaa
     1861 aagcgataaa cggtgctttt tgacatcatg acgcgctgcg acagctcggt tatccctatt
     1921 tcgcgctctt cacccagcgc ctgcaaaatg ccaaaaactt ttagcacgga agatacagaa
     1981 tcaggctgtt tatccagatc tgcgttagcc atttatcacc tcattgcgag tgttttataa
     2041 aaatcagaac tgttttttat tataatttcg caccaggggg gtcgcaatcc atcttttgcc
     2101 ggttagttac aattctgcga catccaccgt gaatatcagt gctagaatca tacccctgtt
     2161 gattattcac caaagatata aaattcctat gccaaaagtt caggccgacg gcctgccatt
     2221 gccccagcga tacggtgcga tattaaccat tgtgattggt atttcgatgg ctgtccttga
     2281 cggcgcaatc gccaacgtcg ccctgccaac aatcgccacg gaccttcatg ccacgccagc
     2341 cagttccatc tgggtagtga acgcctatca aatcgccatt gtcatctccc tgctctcgtt
     2401 ttcgtttctg ggcgatatgt ttggctatcg acgtatttat aaatgcggtc tggtcgtttt
     2461 tctgttgtct tcactgttct gcgccctttc tgattcgctg caaatgctca cccttgcgcg
     2521 tgtcatacaa ggtttcggcg gtgcagcgtt gatgagcgtt aataccgcac ttatccgcct
     2581 gatctatcca caacgttttc tggggagagg gatgggcata aactcgttta ttgttgccgt
     2641 ctcttctgct gccgggccga caattgctgc agcaatcctc tccatcgcat cctggaaatg
     2701 gttattttta atcaacgtac cgttaggtat tatcgccctg cttctggcga tgcgttttct
     2761 gccacccaat ggttctcgcg ccagtaaacc ccgtttcgac ctgcccagcg ccgtgatgaa
     2821 cgcgttaacc ttcggcctgc ttatcactgc gttgagtggt ttcgctcagg ggcaatcgct
     2881 gacgttaatt gctgcggaac tggtggtaat ggttgttgtt ggtattttct ttattcgccg
     2941 ccagctttct cttcccgtac cgctgctacc ggtggattta ctgcgtatcc cgctgttttc
     3001 actttctatt tgcacatctg tttgctcttt ctgcgcacaa atgctggcaa tggtttccct
     3061 gcccttttac ctgcaaaccg tgctcgggcg tagtgaagtc gaaacaggtt tacttctgac
     3121 accgtggccg ttagcaacga tggtgatggc tccgttggca ggctatttga ttgaacgcgt
     3181 acatgcagga ttgctgggtg ctctagggtt gttcatcatg gctgcggggc ttttttccct
     3241 ggttatgctg cccgcgtcac ctgcggatat caatattatc tggccgatga tcttatgtgg
     3301 cgctggattt ggcttgttcc agtcacccaa taaccacacc attattacct ccgctcctcg
     3361 cgaacgtagc ggtggagcca gtggcatgtt aggaacggct cgtctactgg gtcagagtag
     3421 cggcgcggca ctggtggcgc tgatgctaaa tcagttcggt gataatggta cgcacgtttc
     3481 gctgatggct gcggctattc tggcagtgat tgctgcctgt gtcagtggtt tacgtatcac
     3541 tcagccacga tccagggcat aaaaaaagcg cgtcgatcag g
//
LOCUS       DAJVTY010000243         3531 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVTY010000243 DAJVTY010000000
VERSION     DAJVTY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.244"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(21..335)
                     /gene="fliE"
                     /locus_tag="OQQ66_003954"
     CDS             complement(21..335)
                     /gene="fliE"
                     /locus_tag="OQQ66_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1906680.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            550..2208
                     /gene="fliF"
                     /locus_tag="OQQ66_003955"
     CDS             550..2208
                     /gene="fliF"
                     /locus_tag="OQQ66_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS1906681.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2201..3196
                     /gene="fliG"
                     /locus_tag="OQQ66_003956"
     CDS             2201..3196
                     /gene="fliG"
                     /locus_tag="OQQ66_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1906682.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3189..>3531
                     /gene="fliH"
                     /locus_tag="OQQ66_003957"
     CDS             3189..>3531
                     /gene="fliH"
                     /locus_tag="OQQ66_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1906683.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDS"
BASE COUNT          881 a          936 c          992 g          722 t
ORIGIN      
        1 ttaccaacag gttacaaaac ctacacctgc atggacatca cttcctgata cgccgccacc
       61 agcttattac gcacctgaat ccccatttgc atagaaactg aggctttttg catatcggtc
      121 atcacatcgt ttaacgccac gccgggttca ccgagggtga atttttctgc ctgcgtgcgg
      181 gcggctgttt gtgtatcgct tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta
      241 atggtcggtt gcggcagtga atcctgcgca cgcgcactca tcgccgtagc ctgtaactgg
      301 ctgataaccc cttcaatccc ctgtatcgct gacattttcg tctcctggat aatttctggt
      361 agcaaagcct accagtaagt caataagaca aaggcgctaa atagcaacaa aaaaacgggt
      421 ttattggcgg atagaaaaaa acgaaagcac aaataatggc agcgtcaatt tttcgagttt
      481 gctgacccgg gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg
      541 aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgcctg
      601 cgtgcgaatc cgaaaattcc attgattgtt gccggttccg cggcagtggc ggtcatggtc
      661 gcactgatcc tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat
      721 caggatggtg gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc
      781 gaagccagcg gcgctattga agttccggca gataaagttc acgagctgcg tctgcgcctg
      841 gcgcaacaag gtttgcccaa aggcggcgcg gtcggtttcg aactactcga tcaggaaaag
      901 tttggtatca gccagttcag cgaacaggtg aattatcagc gggcgctgga aggcgagctt
      961 tctcgtacca tcgaaactat cggcccggta aaaggggcgc gcgtacatct ggcaatgccg
     1021 aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg catcggtgac ggtaaatcta
     1081 ttacccggcc gcgcactcga tgaagggcaa attagcgcca ttgtacatct ggtttccagc
     1141 gcggttgctg gtctgccgcc ggggaacgtc acgctggtgg atcagggtgg acatctgtta
     1201 accctgtcca ataccagcgg gcgcgatctt aatgacgctc agttgaaata tgccagcgat
     1261 gtcgaaggcc ggattcagcg gcgtattgaa gcgatcctgt cgccaattgt tggtaacggt
     1321 aatattcacg cccaggtcac ggcgcaactg gacttcgcca gtaaagaaca aacggaagaa
     1381 cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc gttcacgcca gcttaatgag
     1441 agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg
     1501 gcacctgcga ataacgcgcc aatcagcacg cctccggcaa atcaaaataa ccgccagcag
     1561 caggcgagca ccaccagcaa tagcgggccg cgtagcacac agcggaatga aaccagtaac
     1621 tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg
     1681 tcagtcgcgg tagtggtaaa ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc
     1741 aacgaacaga tgaagcaaat tgaagatctg acccgcgagg cgatgggctt ttctgaaaaa
     1801 cgcggtgact cgctcaatgt cgttaactcg ccgttcaata gcagtgacga aagcggcgga
     1861 gaactgccgt tctggcaaca gcaagcgttt atcgatcagt tgctggctgc cggtcgctgg
     1921 ttgctggtac tgctggtggc gtggctgctg tggcggaaag cggtacgccc gcagttaaca
     1981 agtcgcgccg aggcgatgaa agctgtacag caacaggcgc aggcccgtga ggaagtggaa
     2041 gatgcggtgg aagtccgcct gagcaaagac gaacaactcc aacaacgacg cgctaaccaa
     2101 cgtctggggg cagaagtcat gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc
     2161 gtggtggcgc tggtcattcg ccagtggata aataacgatc atgagtaacc tgacaggcac
     2221 cgataaaagc gtcatcctgc tgatgaccat tggcgaagac cgggcggcag aggtgttcaa
     2281 gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca atggcgaacg tcacgcagat
     2341 ctccaacaag cagctaaccg atgtgctggc ggagtttgag caagaagctg aacagtttgc
     2401 cgcactgaat atcaacgcca acgattatct gcgttcggta ttggtcaaag ctctgggtga
     2461 ggaacgtgcc gccagcctgc tggaagatat tctcgaaact cgcgataccg ccagcggtat
     2521 tgaaacgctc aactttatgg agccgcagag tgccgccgat ctgattcgcg atgagcatcc
     2581 gcaaattatc gccaccattc tggtgcatct gaagcgcgcc caggccgccg atattctggc
     2641 gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt atcgccacct ttggcggcgt
     2701 gcagccagcc gcgctggcgg aactgaccga agtactgaat ggcttgctcg acggtcagaa
     2761 tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc gaaattatca acctgatgaa
     2821 aactcagcag gaagaagccg ttattaccgc cgtgcgcgaa ttcgacggcg agctggcgca
     2881 aaaaattatc gacgagatgt tcctgttcga aaatctggtg gatgtcgacg atcgcagcat
     2941 tcagcgtctg ttgcaggaag tggattccga atcgctgttg atcgcgctga aaggggccga
     3001 gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg
     3061 cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag gtggaaaacg aacagaaagc
     3121 gattctgctg attgtgcgcc gccttgccga aactggcgag atggtaattg gcagcggcga
     3181 ggatacctat gtctgataat ctgccgtgga aaacctggac gccggacgat ctcgcgccac
     3241 cacaggcaga gtttgtgccc atggtcgagc cggaagaaac catcattgaa gaggccgaac
     3301 ccagccttga gcagcaactg gcgcaactgc aaatgcaggc ccatgagcaa ggttatcagg
     3361 cggggattgc cgaaggtcgc cagcaaggtc atgagcaggg ctatcaggaa ggactggccc
     3421 aggggctgga gcaaggtctg gcaaaggcga agtctcaaca agcgccaatt catgcccgga
     3481 tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc gcttgatagt g
//
LOCUS       DAJVTY010000244         3514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVTY010000244 DAJVTY010000000
VERSION     DAJVTY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.245"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..131
                     /locus_tag="OQQ66_003958"
     CDS             <1..131
                     /locus_tag="OQQ66_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1906684.1"
                     /translation="RYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSA"
     gene            169..1497
                     /locus_tag="OQQ66_003959"
     CDS             169..1497
                     /locus_tag="OQQ66_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1906685.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1544..2869)
                     /gene="rimO"
                     /locus_tag="OQQ66_003960"
     CDS             complement(1544..2869)
                     /gene="rimO"
                     /locus_tag="OQQ66_003960"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1906686.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3082..3465
                     /gene="bssR"
                     /locus_tag="OQQ66_003961"
     CDS             3082..3465
                     /gene="bssR"
                     /locus_tag="OQQ66_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1906687.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
BASE COUNT          906 a          790 c          875 g          943 t
ORIGIN      
        1 cacgctataa ccggatcccg gtgattgcgg aaggcgtaga gagcgaagac gttgcacgcg
       61 aattaattaa attaggatgt gttcaggctc aggggtatct gtaccagaaa cctatgccat
      121 tctccgccta ggataaaagt ggaaaattag taaaagagta gtttacgtat gtccagaatc
      181 aataagttcg tacttacagt cagtctgctg atttttatca tgatttcagc agttgcctgc
      241 gggatctaca ctcaaatggt aaaggaacgg gtgtatagcc tgaaacagtc cgttattgat
      301 actgcttttg cggtggcaaa tattgctgaa tatcggcgta gcgtggcaat tgatcttatc
      361 aacacgctaa atcccactga ggaacagctg ttggttggtt tgcgcacagc ttacgccgac
      421 tcggtttccc cctcttattt gtacgatgtc ggtccttatc tgatttccag tgacgaatgt
      481 attcaggtaa aggagttcga gaaaaattat tgtgcagata ttatgcaggt tgtgaagtat
      541 cgacatgtca aaaatacagg gtttatctct tttgacggta aaaccttcgt ctattacctc
      601 tatccggtaa ctcacaatcg tagtctgata tttttgcttg gtctggagcg tttttcttta
      661 ctgtcaaaat cgctggcgat ggacagcgag aacctgatgt tctctctatt taagaacggt
      721 aaaccggtga ccggtgatga atataatgct aaaaacgcca tcttcaccgt ttcggaagcg
      781 atggagcact tcgcctattt gccgaccgga ttgtacgtat ttgcgtataa aaaagatgtt
      841 tatttgcggg tctgtacatt gattattttc tttgccgcat tggtggctgt gatatcgggt
      901 gccagttgcc tctatctggt acgcagagtg attaatcgtg gtattgtgga gaaagaagcc
      961 atcattaata accattttga acgcgtactg gatggcgggc ttttcttttc ggctgccgat
     1021 gtcaaaaaac tctacagtat gtataactcg gcgttcctgg acgacctgac caaagcaatg
     1081 ggcagaaaat cctttgacga agatttaaaa gcgctgccgg aaaaaggcag ttatttgtgc
     1141 ctgtttgacg tcgataaatt caaaaacatt aacgacacct tcggtcattt gctgggcgat
     1201 gaagtgttga tgaaagtggt gaaaatcctt aaatcacaga tcccggtaga taaaggtaaa
     1261 atctaccgct tcggcggtga cgaatttgcg gtgatttata cgggtggaac gctggaagag
     1321 ttgctatcga ttctaaaaga aatcgttcat ttccaggtgg gaagcattaa tttaagtacc
     1381 agtatcggtg tagcgcattc aaatgaatgt accaccgtcg aacgcttgaa aatgctggcg
     1441 gatgagcggc tgtataagag taagaaaaac ggcagggctc agattagctg gcagtaatca
     1501 ttattcgcag gccggacaaa tgattttgcc cggcctgaat taattaaacc cggctacccc
     1561 acaaatcgta ctcatcggcg tgctcgactt tcacacgcag gatatcaccc ggcttaacgt
     1621 tggtttcacc attgagataa accgcgccgt cgatttccgg tgcatctgcc atgctgcgac
     1681 caatcgcgcc ttcttcgtcc acttcgtcga taatcaccag aatttcacgg cccactttct
     1741 cttgcaggcg ctcggcggaa atctgctgct gcagctgcat gaaacggttc cagcgttctt
     1801 ctttaacttc ttccggaacc tggtcaggca gggcattggc gtctgcacct tcaaccgggc
     1861 tgtatttaaa gcagccaacg cgatccagac gcgcttcttt caggaagtcg agtagcatct
     1921 ggaaatcttc ttctgtctcg ccagggaagc cgacaataaa ggttgagcgt agggtcagtt
     1981 ccgggcagat ttcgcgccac tgtttgatgc gcgccagttg gcgatctaca gaacccggac
     2041 gcttcatcag tttgagaatg cgcgggctgg cgtgctgcaa cggaatgtcc agatacggca
     2101 ggattttgcc ttctgccatc agtgggatga cgtcgtccac atgcggataa gggtaaacgt
     2161 agtgcagacg tgtccagatc cccagtttcg ataactgttc gcacaggctg accatgctgg
     2221 tttttaccgg ctcgccgttg tggaagccag tacgatgttt aacatcaacg ccataggcgg
     2281 aagtatcctg cgagatcacc agaatctctt taacgcccgc atctaccaga cgtttcgctt
     2341 cacttaacac ttcgccaatc ggacggctca ccaggtcgcc gcgcatagac ggaataatgc
     2401 agaaggtgca gcggtgatta cagccttccg aaattttcag ataggcataa tgacgcggcg
     2461 tcagtttcac accttgttct ggcaccaggc tcaggaatgg gttgtgtttc ggttttggca
     2521 cgtagtgatg aacgtgctcc agaacctgct catagctatg aggcccggtg atttccagca
     2581 ctttcgggtg gacttcgcgg atctgatctt cttttgcccc cagacaaccg gtcacaatta
     2641 ccttgccgtt ttcattcaac gcttcaccaa tggcttccag tgattcttgt accgcgctgt
     2701 caataaagcc gcaggtgttg acgatcacca tgtccgcatc gtcatagctc ggtaccacgt
     2761 cataaccttc agtgcggagt tcggtgagaa tacgctctga atcgacaagg tttttcggac
     2821 agccaaggga aacaaagccg attttcggct ggggagttac tttgctcata gcttaaaaaa
     2881 tattcagtta caggaaaggt cagggcaggg attctacaga gttctggata aaatttgtat
     2941 cgcaatctca ttcgctggcg gaggcgaagg aaatgtaaat tttgttaatt cggcgtgaag
     3001 aattgatcct ggacagcatt ttgctcaaaa aatagccata ctatttaatt gcaacaaggc
     3061 tggaaagagg aggatcgaag tatgttcgtt gacagacagc gaatcgatct gctgaaccgg
     3121 ttgatcgacg cacgcgttga cctcgctgca tacgtgcaac tgaggaaggc aaaaggatac
     3181 atgtccgtca gcgaaagcaa tcatctacga gataactttt ttaaactgaa tcgcgaactg
     3241 cacgataaat cgctgcggtt gaatcttcat ctggatcagg aagagtggag tgctcttcat
     3301 catgctgaag aagcattagc gacagccgca gtatgtttga tgagtgggca ccatgattgc
     3361 ccgactgtta ttaccgtcaa cgccgataag cttgaaaatt gtctgatgag cttaacgctg
     3421 agtatccaga gcctgcagaa gcacgccatg cttgagaagg cctgaaaact aagggggaga
     3481 aagcgtctcc cccttcatgt ttaagttttt gtaa
//
LOCUS       DAJVTY010000245         3474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVTY010000245 DAJVTY010000000
VERSION     DAJVTY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.246"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            577..1293
                     /locus_tag="OQQ66_003962"
     CDS             577..1293
                     /locus_tag="OQQ66_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906688.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="OQQ66_003963"
     CDS             1845..2249
                     /locus_tag="OQQ66_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906689.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2946
                     /locus_tag="OQQ66_003964"
                     /pseudo
     CDS             2312..2946
                     /locus_tag="OQQ66_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2988..3197)
                     /locus_tag="OQQ66_003965"
                     /pseudo
     CDS             complement(2988..3197)
                     /locus_tag="OQQ66_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3262..>3474
                     /locus_tag="OQQ66_003966"
     CDS             3262..>3474
                     /locus_tag="OQQ66_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906690.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLR"
BASE COUNT          968 a          731 c          793 g          982 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 ggcctgaggt gtactggcaa tagcggacac caccatttgt tctttttcta agcagtcatc
     3001 tgataatatt ttatcctgaa ggctgccggg gagatattcc ccagacgaga gtgacgacgc
     3061 tgacgattgt agaaaatctc aatgtattcc cgtattactg agatggcctc atcccggctc
     3121 ttaaaacgat agtggctcag gctttcattt ttcagcgttc cccagaagct ttccatcgga
     3181 gcgttgtcgt aacagtttga ggtagcctga gtttaacgga cactccttcc tgaaatagaa
     3241 tggcatcaga aggagccaat aatgagcaga aaaacccaac gttactctac ggagtttaaa
     3301 gccgaagccg tcaaaacggt tcctgaaaat cagctttcga tcagtgaagg cgcttcccga
     3361 ttatctgttc ctgaaggcac tttaggacaa tgggttaccg ctgcaagaaa agggctcaat
     3421 acaccaggct cccgcacagt ggctgagctg gaatctgaag ttatgcaact gcgt
//
LOCUS       DAJVTY010000246         3400 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVTY010000246 DAJVTY010000000
VERSION     DAJVTY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.247"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(10..312)
                     /locus_tag="OQQ66_003967"
     CDS             complement(10..312)
                     /locus_tag="OQQ66_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906691.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(312..1022)
                     /locus_tag="OQQ66_003968"
     CDS             complement(312..1022)
                     /locus_tag="OQQ66_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906692.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1019..3040)
                     /locus_tag="OQQ66_003969"
     CDS             complement(1019..3040)
                     /locus_tag="OQQ66_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1906693.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDVGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3024..3206)
                     /locus_tag="OQQ66_003970"
     CDS             complement(3024..3206)
                     /locus_tag="OQQ66_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1906694.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          796 a          992 c          813 g          799 t
ORIGIN      
        1 gtctgtagct catatcagtt cctcaggtaa cagccagtag agccggtacc gggatccgag
       61 cccccgccag tcagggtcac tattcccctc cgtgtcggta aaaaacaggt ccccctgaaa
      121 cggcaggtat ccgtaccgga caatccggtt attattcaga cacagcacgc catacaggca
      181 cggttcacca ttaacaatga tatcgatata catccccgta gtacgctgat tcaggcgaat
      241 ggtgcagacc tgagcgccca gtgtcaccgt aaactgctgg gctttaacgg gagataaaac
      301 aatttccagc atcaggtgat ccccctgttc gtggcacttc gtctgtcagc gtctgacggt
      361 tgtgtcaccg acgccgtaac tggctgcgtt ttaaccgatg cggccccttt tgctttatcg
      421 ttgtccgtgg gagacgactt atccgtactt cccactgaca cctctcctgt attcattacg
      481 gtctgaaata ccgcagtaac cgtcagtaag gtcggaccat tattacttcg cattcggtag
      541 tcatatttca caaggtcgca ggatgtccat gtcttgtccg gcgtctcaat atcgtagagt
      601 cctgccgtgg tacgcatcct ttcaagcgtt tccagcacat tcgtccgcga ggtggtggaa
      661 aaatttgtca ggttcgggac ggatccggaa aaggccgtcc acccctctac aatgaaagtc
      721 acatgcagtt ccggcgatct ctggatttta ttaaaggtgg tataagcccc ctgctccacg
      781 gggacggtgg aaacagaagc ctccgcgccc acctcaacga caacaaaaga atccggggag
      841 aaaggtttgc cacccttctg atgggcacct gccggatcat tccgtgcgta ataaataccg
      901 aatgacggtg ccagtacact attaatgagt cccaggacac cgccgccacg aactgcactc
      961 agtacgttac tttcactgag cgaaaagtta ttcagggaaa tattatcgaa agaaaaactc
     1021 atcctgttac cccgctggaa taaaccgaca caagcgccga attcgtgata cgccgacgtg
     1081 cgtcatcggt aatacccttc acattgtccg acgttgtggt gacattcagt gtcccgatat
     1141 gcgtggtttc cgttacggtg gactgcgata caggcgcagg atggagcgac cgtatggcca
     1201 ttgccgcacc cggataaggc aggttcgcca gcacccgcgg aatatagttg cgggtctcct
     1261 ccggagcagc agccagtccc ttacgctgaa catttccctc tccccagtta tatgccgcca
     1321 gagccttagc cagatcgcca tgaaaaaaac gtatcaggcc accaagtttt ctcgcggcgg
     1381 catcagcggc tttttcggga tcaaaggcat cgttccccct cagaccaaat tcactggccg
     1441 tcttcggcat gaactgaaac agtcccatcg caccagcagg tgagacggca aactgattac
     1501 cacgggattc ggtgatcgca acgctgcgca gcagtccggg cggcaggtta tatttttcct
     1561 ccagttgaga cagtttcggt tgcagccagc ctaacagggc ttccccggtc ttcgtcggtc
     1621 gctggcggtt ttgcatggca ttaccgagtt tttcctgcgt cgcacgcata ccctgtagcc
     1681 aggattcacc gaaggctgct tccctcccgg ttgcctgaga agcctgagta tccagcatgc
     1741 cctgctgcca tactgtgggt gattgtgccc gggtgatgtt gccaggcttt tctccgacat
     1801 ccagttttgt ctgatacgtc tccatctctt tcttattgaa aaagaaagtc ccgtctgaag
     1861 cccagaaaaa accatgtgaa tccagccagt ccttattttc cctgccaaca atggatgtca
     1921 gtaatccgtc aacaaccggg taaagcgccg ttatcgcaaa aagaaggcct ccggggccac
     1981 tgagggcttt tgtcagcccc cgcacccatg acgccacttt cagcccgatc agcgtaataa
     2041 tgacattctg ccagcccccc agttctccgg cagccttatt cgccagggag gccactgact
     2101 caacgtcatt cagaaatgtg gtgataaacc tgttcacttc ctccggatgt tgctgcatcc
     2161 agtcaccgag tgtctccagc cagtggctga gcttcagcgc gtacggcatc aacgcggtgc
     2221 taatcgtcat cccgatgccg gaccaggtct gatcgagctc aacaagctct tttcgtagct
     2281 tacgggccag cgctgtacgc cccggcgtaa cctgcgaacg ggaagtaaat tcgtccacat
     2341 ctttcagcgc atgaccggag ccaagaaact gctgtccggc ataactgaag cccagcgcgt
     2401 tgccgtaggc agtttgttcg gactttgtca gtcgcggaaa aataaccgcc agcttgcgca
     2461 tgatggcttc agtgctgtca gtatttaaat caacactaat gcccgccctg gctgcaacct
     2521 gaaacaaatc ctgtaacaca ggatcaaagg accttccggc tctgaaggcc gcttttgcat
     2581 ttgcaagccg ggaaaatgcc ccagacattt ccccttcatc aacattattt gcccgcgcag
     2641 cccgtaacca tccgtccaga tttctggcct tcatgccaaa agcatcggat tccacggaca
     2701 gacggttcag atcacgggca aaacctgtta ccaggttctt cagccccccc agcgtcagac
     2761 tgacacccgc aagagccaga atctcgctac ggatccctga aaaaaaacgg gacgcccgtt
     2821 tcccggcatc ttccattcga cgggccgttt tatcagcatc ttttccggtt ttttccagtg
     2881 attcactggt tttcttctgt ccctgactga atgtgtcagc gacaccttcc atcaccgtcg
     2941 tgagccggtt aagggcagca tgtactgcct gttccccggc agtaaagtcc ctgtcatcaa
     3001 tcccgaggga cagaacaagc tcatcaagta cctgcgccat ttctgttctc ctgtatcacc
     3061 tgttcgttat gggcatccac atgaattatt tccagcagat cccataagtc ctgcacacca
     3121 aggacagaat ccagttcagc ttttgaagcc ttaccggagg agataacggt cgcaatggta
     3181 cggggaacat taacgtaatc caccaccccg aacggtctgt cggggtcgag ataacgcggg
     3241 gggatatcta gctgacgacg gtagcgaaaa aatccacatg cagcttaaag acctccgcac
     3301 gcagattaag acgggtaatg atttcttcaa tatcctcttc aacaagcggt ctccggatgc
     3361 tgcgtttttt cggatcggga acaaactgca cacagtccat
//
LOCUS       DAJVTY010000247         3399 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVTY010000247 DAJVTY010000000
VERSION     DAJVTY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.248"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(145..795)
                     /gene="tetR(A)"
                     /locus_tag="OQQ66_003971"
     CDS             complement(145..795)
                     /gene="tetR(A)"
                     /locus_tag="OQQ66_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1906695.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            901..2100
                     /gene="tet(A)"
                     /locus_tag="OQQ66_003972"
     CDS             901..2100
                     /gene="tet(A)"
                     /locus_tag="OQQ66_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1906696.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2132..2992)
                     /locus_tag="OQQ66_003973"
     CDS             complement(2132..2992)
                     /locus_tag="OQQ66_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCS1906697.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
     gene            complement(3001..3399)
                     /locus_tag="OQQ66_003974"
                     /pseudo
     CDS             complement(3001..3399)
                     /locus_tag="OQQ66_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          628 a         1055 c         1057 g          659 t
ORIGIN      
        1 gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc
       61 gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg
      121 gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct
      181 gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc
      241 tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg
      301 agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag
      361 cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga
      421 aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc
      481 cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa
      541 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg
      601 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct
      661 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt
      721 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg
      781 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta
      841 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac
      901 gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc
      961 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac
     1021 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt tgcctgcgca
     1081 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct cgtctcgctg
     1141 gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg ggttctctat
     1201 atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt
     1261 gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc
     1321 gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac
     1381 gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt
     1441 ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc
     1501 gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc
     1561 atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc
     1621 tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca
     1681 ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc
     1741 atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga
     1801 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg
     1861 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg
     1921 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg
     1981 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc
     2041 tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga
     2101 tcgtggaaac gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg
     2161 ccctaggagt gcggttggaa cgttggccca gccagatact cccgatcacg agcaggacgc
     2221 cgatgatttg aagcgcactc agcgtctgat ccaagaacaa ccatcctagc aacacggcgg
     2281 tccccgggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     2341 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     2401 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     2461 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
     2521 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
     2581 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
     2641 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
     2701 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
     2761 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
     2821 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
     2881 ccgccggcaa cgcccgcagc atcgcgaccg tcatcggtga gaagttcggc aggtattggg
     2941 tggtgacaat gtaggtgctg ccccaaatgg caggtgctat cgctgtgaac aataaatcgg
     3001 ggcagacggc gcttccagcg tgacgaccat gccgagcacg cagcgggtga acacgtcgat
     3061 ggcgagggtc aggtacgggc ggccaatagg ttgccggtcg cggtcatcga ccacgatcag
     3121 gtcgatgacc gtatggtcta tctgctccag cggcgcggtc acggcaggag gctcgccgcc
     3181 cacaccttgt aggtcacgag cggcatcctg gccttcccgc cggcggatga ccttgcgcgg
     3241 gtcaaggcta gcgatccgta aggccacggt attgcgcgcc ggcactcgca gtttttgagc
     3301 cttgcacacc tgagtgactt cgcggtgaaa ggccgctagg ctgcgcttct gcttggtcag
     3361 gaaccgcttt tgcagtagct cgtggatgac gcgctcgac
//
LOCUS       DAJVTY010000248         3299 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVTY010000248 DAJVTY010000000
VERSION     DAJVTY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.249"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            217..516
                     /locus_tag="OQQ66_003975"
     CDS             217..516
                     /locus_tag="OQQ66_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1906698.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            611..1522
                     /gene="glyQ"
                     /locus_tag="OQQ66_003976"
     CDS             611..1522
                     /gene="glyQ"
                     /locus_tag="OQQ66_003976"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1906699.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            1532..>3299
                     /gene="glyS"
                     /locus_tag="OQQ66_003977"
     CDS             1532..>3299
                     /gene="glyS"
                     /locus_tag="OQQ66_003977"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1906700.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYACHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNI"
BASE COUNT          786 a          871 c          898 g          744 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag cattacctgc atctgtgtct ctgtatctgg
       61 ttaaaaaaag tctgaccgat aaatcatttg gttggcgcac tggaataatc tgagttttat
      121 cactacagct tatagaggct taaggaaatt cgtaagatat cagccactat accgatataa
      181 ataataagac tcacctgcaa accagacggt aatttaatga tgatgaacgc tttctttccg
      241 gcaatggcgc ttatggtgct agtgggttgt tctacaccgt cacccgtgca gaaagcacaa
      301 cgggtaaagg ttgatcctct gcgttcgttg aatatggaag cgttatgcaa ggatcaggcg
      361 gcaaaacgtt ataacaccgg cgagcaaaaa atcgacgtca ccgccttcga acagttccag
      421 ggaagctatg aaatgcgcgg ttataccttc cgtaaagagc agtttgtctg ttcttttgac
      481 gcagatggcc attttttgca tctttccatg cgttaagccc tgctttttcc cgtttcgtac
      541 tgtatatctt ccatccagcg ggtatactga tcccttcctt taaatccaca cgtatccagc
      601 acgaaataat atgcaaaagt ttgataccag gaccttccag ggcttgatcc tgaccttaca
      661 ggattactgg gctcgccagg gctgcaccat tgttcaacca ttggacatgg aagtcggcgc
      721 gggaacctct cacccaatga cctgtctgcg cgcgctgggg ccagaaccga tggcggctgc
      781 ttatgttcag ccttctcgtc gcccgaccga tggtcgctac ggcgaaaacc ccaaccgttt
      841 acagcactac tatcagttcc aggtggtcat taagccatcg ccggacaata ttcaggagct
      901 gtacctcggt tctctgaaag agctgggcat ggacccgact attcacgaca tccgtttcgt
      961 ggaagataac tgggaaaacc cgactctggg tgcctgggga ctgggctggg aagtgtggct
     1021 aaacggcatg gaagtgacgc agttcactta cttccagcag gttggtggtc tggagtgtaa
     1081 accggttacc ggcgagatca cctacggtct ggaacgtctg gccatgtaca ttcagggcgt
     1141 agacagcgtt tacgacctgg tctggagcga cggcccgctg ggtaaaacca cctacggcga
     1201 cgtgttccat cagaacgaag tggagcagtc cacttacaac ttcgaatacg cggatgtgga
     1261 cttcctgttc acctgctttg agcagtacga gaaagaagcg cagcagctgc tggcgctgga
     1321 aaatccgctg ccgctgccag cctacgagcg tattctgaaa gccgcccaca gcttcaacct
     1381 gctggatgcg cgtaaagcca tctccgtcac cgagcgtcag cgctacattc tgcgcattcg
     1441 caccctgacc aaagcagtgg cagaagcata ctacgcttcc cgtgaagccc tcggcttccc
     1501 gatgtgcaac aaagataagt aagaggcggc tatgtctgag aaaacttttc tggtggaaat
     1561 cggcactgaa gagctgccac caaaagcact gcgcagcctg gttgagtcct ttgctgcgaa
     1621 ttttactgtg gagctggata acgctggcct cgcacacggc accgttcaat ggtttgctgc
     1681 tccgcgtcgt ctggcgctga aagtagctaa cctggcggaa gcgcaaccgg atcgtgaaat
     1741 cgaaaaacgc ggcccggcga ttgcccaggc gttcgacgct gaaggcaaac cgagcaaagc
     1801 ggcagaaggt tgggcgcgtg gttgcggtat taccgttgac caggctgagc gtctgactac
     1861 cgataaaggc gaatggctgc tgtatcgcgc ccatgtgaag ggcgaaagca ccgaagcact
     1921 gctgccgaat atggttgcga cttctctggc gaaactgccg atcccgaaac tgatgcgttg
     1981 gggcgcaagc gacgtgcact tcgtgcgtcc ggtgcacacc gtgaccctgc tgctgggcga
     2041 caaagtcatt ccggcaacta ttctgggcat tcagtccgat cgcgtgattc gcggccaccg
     2101 ctttatgggc gaaccggaat tcaccatcga caacgccgat cagtatccgg aaattctgcg
     2161 tgagcgcggg aaagtcatcg ccgattacga agaacgtaaa gcgaagatta aagccgatgc
     2221 cgaagaagca gcgcgtaaga ttggcggtaa cgctgactta agcgaaagcc tgctggaaga
     2281 agtggcttcg ctggtggagt ggccggtcgt tctgaccgca aaattcgaag agaaattcct
     2341 cgcggtgccg gctgaagccc tggtttacac catgaaaggt gaccagaaat acttcccggt
     2401 gtatgcgaac gatggcaaac tgctgccgaa cttcatcttc gttgccaaca tcgaatcgaa
     2461 agatccgcag caaattatct ctggtaacga gaaagtcgtt cgtccacgtc tggcggatgc
     2521 cgagttcttc ttcaataccg accgtaaaaa acgtctggaa gataacctgc cgcgcctgca
     2581 aaccgtgctg ttccagcaac agctgggtac gctgcgtgac aaaactgacc gcatccaggc
     2641 gctggctggc tggattgctg aacagattgg cgctgacgtt aaccacgcaa cccgtgcggg
     2701 cctgctgtct aagtgtgacc tgatgaccaa catggtcttc gagttcaccg acacccaagg
     2761 cgttatgggg atgcactacg cgtgtcacga tggcgaagcg gaagacgtag ccgtcgcgct
     2821 gaatgagcag tatcagccgc gctttgccgg tgatgacctg ccgtccaacc cggtagcttg
     2881 tgcgctggcg attgctgata agatggatac tctggcgggt atcttcggta tcggtcagca
     2941 tccgaaaggc gacaaagacc cgtttgcgct gcgtcgtgcc gcacttggcg tgctgcgaat
     3001 tatcgttgag aagaacctca accttgatct gcaaacgctg accgaagaag cagtgcgtct
     3061 gtatggcgat aagctgacta acgccaacgt ggttgatgat gttatcgact ttatgctcgg
     3121 tcgcttccgc gcctggtatc aggacgaagg ttacaccgtt gacaccatcc aggcggtact
     3181 ggcgcgtcgt ccgactcgtc cggctgattt cgatgcccgt atgaaagcgg tatcgcattt
     3241 ccgtaccctg gatgcagctg ctgcactggc ggcggcgaac aaacgtgtat ctaacattc
//
LOCUS       DAJVTY010000249         3208 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVTY010000249 DAJVTY010000000
VERSION     DAJVTY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.250"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..892
                     /locus_tag="OQQ66_003978"
     CDS             <1..892
                     /locus_tag="OQQ66_003978"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1906701.1"
                     /translation="AGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLS
                     AVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNA
                     ITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRDR
                     LNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDIS
                     GENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTN
                     NPDLSIANVISLVENAERLN"
     gene            859..1818
                     /gene="psuG"
                     /locus_tag="OQQ66_003979"
     CDS             859..1818
                     /gene="psuG"
                     /locus_tag="OQQ66_003979"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1906702.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1912..3161
                     /locus_tag="OQQ66_003980"
                     /pseudo
     CDS             1912..3161
                     /locus_tag="OQQ66_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
BASE COUNT          766 a          723 c          867 g          852 t
ORIGIN      
        1 cgccggatat tcacatgaat cattaaatta tgcggattca aatccaggta aaataaaatt
       61 tacgcctggt ggagtcgggc gcaatattgc acaaaacctg gcgttgctgg ggaacaaagc
      121 ctggctactg agcgccgtag gcagtgactt ttatggtcaa tcgctgctaa cgcaaaccaa
      181 tcaatctggc gtttatgtcg ataaatgcct gattgtgccg ggagaaaata cgtctagtta
      241 tttatcatta ctcgataata ccggtgaaat gctggttgct ataaatgaca tgaatattag
      301 caacgctatt acagctgaat atctcgcaca gcaccgtgaa tttattcaga gggcaaaggt
      361 cattgtcgcg gactgtaata tcagtgaaga ggcactggca tggattctgg ataatgccgc
      421 caacgtaccc gtatttgtcg atccggtttc cgcatggaaa tgtgtcaaag tgcgcgaccg
      481 tctaaatcag atccacactc tgaagccaaa ccgccttgaa gcggaaaccc tgagtgggat
      541 tgcgctgtca gggcgtgaag atgtggcaaa agttgctgcc tggttccatc aacatggcct
      601 gaaccgactg gtattgagca tgggcggcga cggcgtttat tacagcgata tcagcggtga
      661 aaatagctgg tctgcgccga tcaaaaccaa tgttattaat gttaccggag cgggcgatgc
      721 catgatggcg ggacttgctt cgtgttgggt agacggaatg ccgtttgccg aatctgttcg
      781 tttcgcacag ggatgttcgt caatggcgct ctcctgtgaa tacaccaata accccgattt
      841 atcgattgcc aacgttatat cgttagtgga gaacgcagaa cgtctgaatt aaaaatttcc
      901 cctgaattat tacaaatttc cccggaagtg caggacgctt taaaaaacaa aaaaccggtt
      961 gtggcgctgg aatcgaccat tatttctcac gggatgccgt tcccacaaaa tgcccagacc
     1021 gcaattgaag tagaagaaac tattcgtaaa cagggcgctg tacctgccac tatcgccatt
     1081 attggcggcg tgatgaaagt gggtttaagc aaagaagaat ttgaattact gggtcgtgaa
     1141 gggcataacg tgaccaaagt tagtcgtcgc gatttacctt ttgttgttgc cgccggaaaa
     1201 aatggcgcaa ccactgtggc ttcaacgatg attattgcgg cgcttgccgg aattaaagta
     1261 tttgccaccg ggggaattgg tggtgtgcat cgcggggcgg aacatacctt cgatatttct
     1321 gccgatttgc aagaactggc aaatactaat gtcaccgttg tttgtgccgg ggcgaaatct
     1381 attctcgatt taggattaac cactgagtat ttagaaacct tcggtgtgcc gttaattggc
     1441 tatcagacta aagcgctgcc tgcgtttttc tgccgtacca gctcgtttga cgtcagtatt
     1501 cgtctcgaca gcgccagtga aattgcccgt gcaatggcgg tgaaatggca aagcgggctg
     1561 aacggtggcc tcgtggtagc gaacccgatc ccggaacagt ttgcgatgcc ggaggaatct
     1621 atcaatgcag ccatagatca agccgtcgcc gaagccgaag agcagggcgt tattggtaaa
     1681 gaaagtacac tgttcctgct ggcgcgcgtt gctgagctga ccggcggtga cagcctgaaa
     1741 tccaatatcc agctggtatt caacaacgcc attctggcga gtgaaattgc caaagaatac
     1801 cagcgtctcg cgggttaatt tccgacgggc agaaagccgt cgcccgtact catcaagcat
     1861 accaaaacct ggcgctgacg ccgggtttac tggcatgaag ggaaaatcat tatggatata
     1921 atgagaagtg ttgtggggat ggtggtgtta ctggcaatag catttctgtt gtcagtgaat
     1981 aaaaagagca tcagtttgcg cacggttgga gccgcactgc tgctgcaaat cgctattggt
     2041 ggcatcatgc tctacttccc accgggaaag tgggcagtag aacaggcggc attaggcgtt
     2101 cataaagtga tgtcttacag tgatgccggt aggccttcat ttttggttcg ctggttgggc
     2161 cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc gtacttccgg
     2221 cgattatttt cgttactgcg ctcatcagtc tgctgtacta cattggcgtg atggggctgc
     2281 tgattcgcat ccttggcagc attttccaga aagctcttaa catcagcaaa atcgaatctt
     2341 ttgtcgcagt cacaactatt ttcctcgggc aaaatgagat cccggcgatc gttaaaccgt
     2401 ttatcgatcg catgaatcgc aacgagttgt ttaccgcaat ttgtagcggg atggcgtcca
     2461 ttgctggttc gatgatgatt ggttatgccg gaatgggcgt accaattgac tacctgttag
     2521 cggcatcgct gatggcgatc ccaggtggta ttttgtttgc acgtattctt agcccggcca
     2581 ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg ccaaaaagct
     2641 ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct ggtgtagcga
     2701 cggtggtaat ggcatttgtc gcaattattg cgctgatcaa cggcattatc ggcggaattg
     2761 gtggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat gtgctggcac
     2821 cgctggcgtg gatcatgggt gtggactgga gtgatgccaa tcttgcgggt agcctgattg
     2881 ggcagaaact ggcgattaac gaattcgtcg cttacctgag tttctcccca tacctgcaaa
     2941 cgggcggcac gctggaagtg aaaaccattg cgattatctc ctttgcgctt tgtggttttg
     3001 ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg ccaaaacgcg
     3061 cgccggaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt tccaacctga
     3121 tgagtgcgac tattgccggg ttctttattg gtttagcttg agtttcattg ccggatgtac
     3181 gcgcttatta agcgttgcat ccggcaat
//
LOCUS       DAJVTY010000250         3175 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVTY010000250 DAJVTY010000000
VERSION     DAJVTY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.251"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..2498)
                     /locus_tag="OQQ66_003981"
                     /pseudo
     CDS             complement(1..2498)
                     /locus_tag="OQQ66_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          793 a          740 c          734 g          908 t
ORIGIN      
        1 tttgcggctc tatatagaac ccataaccag taggggacag gttgaacctc tgcccggctt
       61 ctagggagat gctcattcca ttcgcagtgc cgttggcgtt aacgccgttg ttatgactgt
      121 ccagtacgtg gaaactattt ttctggcgcg atgcttttat aacgagatcg ctgtaaaaac
      181 cgttttgtgc catgtaactg gcgtacattc ccatgtagtc tgaacgtgcg gtaccgtcgc
      241 ctccgctata gtccggcgat gcatgtgttg agccaatata cagaccgaca tacaacggca
      301 tttcatcaga gagacgttta tccccaccaa actggatacc gctgtaaccc atgtcaaagc
      361 cgctcagttt gccactggca aaggagtcta ggcttccccc ataactgcgc aaccagatat
      421 taccgtcttt actctgattt cgcaggtcac ccatccgttg catcagcgta cggttttcaa
      481 catagttcaa taagtaaccg acattgagat aattgccgcc agcatctgcg gtcgtggtgg
      541 gtttaggcgt gggagcgggt tcaggcgtag gatcggggtt gactatggga ggctgagcgg
      601 gagctggtgt gggttcagga ttaggagtcg gttccggaac tgtcccggaa gcgtaaagct
      661 cccagttagt gccattttta cgcacatcgt acagatatcc cccccaactc aacctgagaa
      721 gacgcgctga acgaggccgc gccgtcagtg gtttttacca ctgtcagaac ttcatttccc
      781 gttgtggcct cgctgccctg gttgcggata gccaatacgt gattaccagc actactcccg
      841 ctgatattca ataaatcccc tttattatta acgccattac cctcgccaac aacatcagca
      901 cgcataataa aggtactgtt accgctcagg ttctctacgt ttaatgtggc aaatgtgccg
      961 gcagttgacc cgtggctggc aaaatcgaca gttgaatggc tcagcgccag cgtgtcgaga
     1021 ttagaattac ttgttacgtt ccagacgctg ttattcattg caacgtccag ttttccgcca
     1081 ttgacattat cgctgaggga ggaacctgtc caaaccgacc cagggtgcat atccagattg
     1141 attagcccac ctttggaaaa aacgctaccg ttgataagca tacgacccga ggcgttgatg
     1201 cggctggcgg cataaccatc gtcatgttgc gttgcaatgg ccatctggtc gggtgtactc
     1261 atatcaatga ccagatcgct cgtgaggtcg atctggctgt tggtcatggc ataaattccc
     1321 ctggctcctg ccgcgccggt gatagccaaa ctgtctccgg ttattctacc gccgctgagc
     1381 gcccacaaac ccagcgccag actgccatta cgatcaactg taatatcggt attttgcatg
     1441 ttgacaacag ccgttgccgt ctgggccgag gcaccataag aaccgccgga aaagatgctg
     1501 tttcgttgcg ctgccgtgcc agaaaaattg attgtcgcgt ctgaaccact ggtgacgagg
     1561 ccaccgccct gcgcggaaga aatatggcta tctgcaccga tagtggttgt accaccacga
     1621 acttcgacgc cattggccgc ggctccagtt acatcaacag tgagtgcatt cgcgctaacc
     1681 tggccaaagc tccagaggcc gtgtgcatta tcgccattgg ttttaatgga actgtttgtt
     1741 ccgagatcga caacagagtt tttctgtacg tttatcccca tggcgctgta gccctgaaca
     1801 tcgattgtca ggtctgtagc cgtaaaacgc gcagcaccat tggcgttatt gccgttgaga
     1861 ccaccgatat aaacacctgt acttccatcg gtcgtgattt tacttccgct tccaagatcg
     1921 acactggtgc catagtcatt gatggttagg cctataccgt tcgagttttc aatggtgaat
     1981 tgagtggctg ttagtgttga gctatgccca ataatgatgc cgtcatcgtt actcttaatg
     2041 gtgctgcctg tgcctaagtc agcatgggta tagtcaccaa ttaagttaat gccgatggca
     2101 gaggtttgac caacaacatc tactgtgagt cggttagctg tcagggagct gtttttgccc
     2161 tgaataatga ttccttttgc atatccagag gcgtcgttga cattgacggt gacatcatta
     2221 cccaggttga tattgccagg cgtgtttcct gctggggtca ttacaccata gaggtttcct
     2281 gatggatcgg cggtatctcc tgtaattctg tcaccatcat taatagtaat attggtgcca
     2341 tattgacttc ctgtatcagc agctatcagc aggggggaaa agataattgc attcaaacca
     2401 atagcgccaa attttatcag ttgaattgag ggcgaacgga tttttaattc agcgagacga
     2461 tctgctttgg tattcccatt gtgttgtttg atgcccatac cactcctata tagtacccag
     2521 actatgggtc tatggatttt gtcattttgt ttaaatcaat gaataatatc ctcttatcat
     2581 catgatattt gtatggtgca atcatgatga gcttctcaga gaataattct ctattaccgg
     2641 gcaaaatcag gaacacagga attaatatat atttaacatc ccccttacaa aggtgagttt
     2701 atatcgagtt attccctaag ttgtcgttac ttattccaat tcgttactat aaatatggca
     2761 aaaatattac aacaaacagc ggttaaaaaa tttggtgaag attttttgta ataatcctaa
     2821 ttagttatgg gaatttcgct tgatgtatct gcataacaaa aacaatatgc gccatttttg
     2881 cattactaat aagaaaaagc aaaaaacatc gatattcatt tttggtcaat gggttttctg
     2941 gcctatcgtt gttattgtgc tactggttat cattttcatc ttgccagcat attggagcgt
     3001 gatcaatttt gatcagctgt gaaggtggct gatgtgtagt gatacaaaaa cttacgttat
     3061 atcactgcta atgagatatc cgtcatctga acacttatgt ctttaattat tctcgtggtt
     3121 cactataggc aataagcaca aaagtgtagg atgttacaag aatgattagg actcg
//
LOCUS       DAJVTY010000251         3174 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVTY010000251 DAJVTY010000000
VERSION     DAJVTY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.252"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            346..505
                     /locus_tag="OQQ66_003982"
                     /pseudo
     CDS             346..505
                     /locus_tag="OQQ66_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(723..2813)
                     /gene="iha"
                     /locus_tag="OQQ66_003983"
     CDS             complement(723..2813)
                     /gene="iha"
                     /locus_tag="OQQ66_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCS1906703.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          838 c          732 g          809 t
ORIGIN      
        1 ctgacctctc agtaaatatc aatacggttc tggcgagccg cttacgtgca gcagtcttat
       61 ccggcagttt aaaggggagg acggagacca atgaggcact ggagcagatt cagtgcagag
      121 gtgacctgct ttatcaaact taattgtgac aaaatggcga ttgagaaggt gcaattgact
      181 tatccggatt cggtcgtggc gaggtggtgc ccctgcattc tgctaaccgg gttatgagcc
      241 aggagtcagc gatagacaaa gggacttatc tgaccgggcc ccggaaaaat ggcgggagag
      301 aatgatacct tcctgctgac gagaccggac tttgagatga cgagcgatag tgcggcagtg
      361 atgtacagcc tgatcggtag ctgcaaaact taacggaatc gagccggaaa cgtggctacg
      421 ccacgtgatc agtgtaatca acacctggcc tgccaaccgc atgaaagagt tgttgccctg
      481 gaacgtcact ctctctgtaa actaattttt accccacgtc cttcacggtg cgcttacact
      541 ctggacaagt atgtaaatga taagttaaaa ataatccgtg catttcctgt gacgatatta
      601 gttgttggta acgaaatctc attagcggat cggaaatgca cttaacctaa tacgttgcca
      661 ctgttccgcc aggagttgca gtggcaacgt attctaccgt cagtgatagc gttttgttat
      721 tatcagaact gatagttcag cgacatccag taatttcgct caggtatcac atatcctgtt
      781 gttgatgatc ccgtctggaa gtaatcaccg gcatacagcg tactcttacc ggcactgtac
      841 aggctcacgt cactgtaatc cttgttgagc aggttattca ccgcagcatt cagcgtcagg
      901 gcatccgtca tcttccagga cagacctgca tccaccaccg tccaggcttt caggtattct
      961 cctttctcat catacacttt cttctgtaca gcgctcagtg acgaataatt ctgggtgaaa
     1021 cgtggtgttt tcccgcgata acgggcaccc agccatgatg ccacctcttc ggtgatctgc
     1081 cagttcagtt tcgcattcac catgtgttca ggggtataac tcagcggcgc acctttgtta
     1141 tcaccatcac gttgttcact tcgggtccag gtgtaattca gtgacagcgt gacatcctct
     1201 gaccacagcg gcaatgtgcc ggcaaattcc acaccgtgca accgggcctt tccgctgttt
     1261 acatagctat tggtgttatc atttatggaa taagagacaa tcttgttgga gaagtcagtc
     1321 ataaaacctg tgacattggc attcagaccg gcggggttat cgtaatacac cccagcctca
     1381 taactgacgc tctcttccgg cttcaggtcg gggttaccaa gtagatttgt ttttccctgc
     1441 ccggacacac cactaatccc tttatgtagc tgccccattc tgggtgcctt atatcccgtg
     1501 gtcacaccgc ctttcagcgt ccaggcatct gccacatccc agaccagata tgcacgcgga
     1561 ctgaagtgtc ccccgaattg ctcatgatgt tcatagcggc tgcccgcagt cagcgcaagt
     1621 gcatccgtga gatgccactc atcctcagca aataccgacc agcttttctg ccggaaagtt
     1681 tcacctgtgc tggcaaggac aactccgtct ttcatggacg agctctgaaa ctcgcccccc
     1741 accgtaacca gatgagattc tcccagaggg gtaagcagta atgaattcag gataaggttc
     1801 gattccttaa gctcccgcgg ctgaccggca agcccccatt tgtcgcgctt cagtacactg
     1861 cgtacgagct cacgaccttt attttctgtc tcgttccagt tcagatacga tttccatgtt
     1921 ccgaaggtga aagtatgatc atagccagct gaaattttgt tccgctcata gcgcagggtc
     1981 cggtcatatc cccccgtcag actccccagt tgcccatccc ggttatcata acgctgccgg
     2041 gtggtatcca tatcaaacca gagcacatcc tgctccgacg ccttccagtc aagacgtgca
     2101 ccaagattat aattctgtga ctccgtggga taaggaatac gcgtggctgc tgtatcgctc
     2161 agtgatgtga ccgatgagcc ctgacgctgt tgtgtgctac cgcgtacctg caggctgaca
     2221 gaatcatcca caagggggcc actgctccag aaattaaact ggctgctgtt accccatttg
     2281 ttgctttcct gcagattcag ccctgcattg acggaagaga gccatttgtc tgcattcttt
     2341 ctggtaatga tattcaccac accgcccatc gcatcagagc catacagtgt ggacatcggc
     2401 cccctgataa cctcaatacg ctcaatggcg gccagagggg gcatgaaccc ggtattcatg
     2461 gcagaaaaac cgttgggagt cacgtcactg cttccgccct gacgaacacc atcaatcagt
     2521 atcagcgtgt aactggctgg cattcctcgg atgctgattt ccagcccacc ggttttaccc
     2581 gtaccacttt caacatccac accctctact gatctcagag cctccgccag atcgtggtac
     2641 tggctggact gcaattcctc ctggctaatc acagaaacac tggcggctgc gttagtcagc
     2701 tttttctcat agccggatgc cgagacaatc atcacatcct ctgcagcagc tatgctgctg
     2761 gctgaaaatc cgagacaggg aatgactacg gaagccagag tggttattcg catattacta
     2821 atctccgatc atgttaaccg gcaagacaat acacaaacac aaacaataat gagaataatt
     2881 tatatttgcc ttttacaaaa aataaagatt attaacaaaa ctaaatataa ataacaactg
     2941 cctcggcatg ccagttaaca gctatccgta tggcgtgttc ggttttgcga ataaaaaatg
     3001 gttactgtag aagcggattt ccaccacatg tcaaccatga atggtcagtg gtgcgagacg
     3061 actgggatga tttggtcgat gacttcttcc ggtgaatact gatagaaaca ttgaaatatt
     3121 gtaagagaaa gcgttcaaaa ttctgtggca cgtgaaaaat tctacagcgt catc
//
LOCUS       DAJVTY010000252         3157 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVTY010000252 DAJVTY010000000
VERSION     DAJVTY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.253"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(62..829)
                     /locus_tag="OQQ66_003984"
     CDS             complement(62..829)
                     /locus_tag="OQQ66_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906704.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(832..1542)
                     /locus_tag="OQQ66_003985"
     CDS             complement(832..1542)
                     /locus_tag="OQQ66_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906705.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1558..3063)
                     /locus_tag="OQQ66_003986"
                     /pseudo
     CDS             complement(1558..3063)
                     /locus_tag="OQQ66_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          870 a          766 c          697 g          824 t
ORIGIN      
        1 aatggcacga taacaaaacc gagaattaca gggggaatat aaagccggaa tctaatcatt
       61 tttattttcc tagtgattcg gatgatgcgt catacatcgc gcataaacac tcatcaatta
      121 tccagtcatc ttcccagacc tgatattctg tcattggttc gggctgtttt agcgggttct
      181 caatcgcttg ccgcgctctt tctttcagta aaggatgatt atttctagcc caggcattga
      241 tttgggtggt aatgacgtta tcgatatcgt cctggttatc tctgatataa tcctcacaat
      301 atctgttcaa taaacaggca agagaaacgg tcgaccaaaa gccgggaact gaattaatgg
      361 catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa catttccagc atatactgta
      421 attcgacagc ttcatcgccg gatacccaat cagtcatata ctggcaggca aatcgctgta
      481 aaattgcccg ttcatccgca cgccagtttt ctttgttggc aaggttgata cggcgcagag
      541 aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac aaattccagt aaacgcggca
      601 gaaaatagcg gacctcatcg ctattttgcg gatcaggccc tgggctacta ttccaggcat
      661 tgattaagga atacgggatc gctcgtaacg atgtactccg taatgccttt tgttcctgct
      721 gactcaggca atattcacaa caaaccacaa atttctgcgg cacccgataa cgacgaaagg
      781 tgtgataaat ctgctcaatt aatgcctgca tggcggggct gggttgcata gttaccccct
      841 cgcccgctga tcgcgtgtaa acggttgata gacaaaacga gcattcgccg cgttcaggcg
      901 aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg caactattgc agtctcgaca
      961 gctttcctct gcgcaatttt ccactgagta atgacgcgtg tagagtttca gttcggcctg
     1021 cttatcgcga aatccgcgta aactgttatt gttatgaaat ttccaggcat ctttaaagac
     1081 ctgaaagata tagacatcct caatccagga gtcacatttt ttaagcgggc gctttgctat
     1141 tatcacccgc tcatccggat ttagcatcgc cgtaaattgt acggtgacgt catttgtcgt
     1201 gttcaggtca cattgaacat ctggtacatc actgatatag gggtcaaatc caaaatcagc
     1261 accacaggca caaagacggc taatggtggc ggtatcgagg gaaaatcccg gccatacttt
     1321 gctggaggtt aagccaatat ttaatgtgac atcatatcga agcaatccct caagaccgtc
     1381 agcaccgacg aacttttccc cttcatccag ggcctggtta ataagttgct gaacaatgcc
     1441 ttcatcccag tatgccgatt tgcagttggc aatcgtaata tccacgcctg tgaagggatc
     1501 agtaccggca aggcttgcaa ataatgtatt aactaattcc atttttatta tccagcttta
     1561 aggtagcgtc ttatacatcg ggacataacc tacatcttcc accaaactct gtccctgcgg
     1621 cgttaaaaac cactccaaca atttttgtgt ctctgacgtc gtattttccc gcgttaccat
     1681 aaaggcatcg atgatatagg gatatttgcc gttgcgaatg ttttcggcag tcggtgcaat
     1741 accgttaatc gctagcagtt ttatattttt atcggcgttc atttgtgttg cgtaataacg
     1801 gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg gcaacgactt taatcattcc
     1861 ctccatcata cttgccactt ctgtttcctg cggcgagatc atgcggacat ttttcatgac
     1921 ctgggattgc atcaccgtct ggctaccaga gtcttccggg cgttgctagg tctggatctc
     1981 ctgatcgtta ccgccaacag tacgccagtt ggtaattgca ccactgaaga tgtcacgcac
     2041 ctgttgttca gtcagggaat taaccggatt atccgcattg acgatgaaaa caaacgcttc
     2101 acgggcaaaa ggcgtgtaca gcaaagtaat gcccgattcc tcagcgcgtt ttttctgccc
     2161 accggaaggt tgcgccacaa aaataatatc agcatcacct ttaacaatcc tgttatacgc
     2221 atcgggggta cgagagctct ccagatattc cctggtgtga aaatcttccg gtataacgct
     2281 caaagcataa aatgcagagg cataaattgg gtacgccgcc gtagcaccat cgagtcgcgg
     2341 ccagttttgc gtaaactgaa tttgcggttt accacgcagt ggtgtcagtt gattattgag
     2401 tttatcgggt cgccaggccc aggtatcaat attttcgcgc acaccgacgc cgggattgag
     2461 ctgtttatat aaataaccct gataaccagc gacaaaacca agcatgagaa ggataaacag
     2521 gagggaatta cggcaacgta gcccggatgt accggttaaa ggatgtctgg ttggccggcc
     2581 gtaccccagc gaaaaactga tatgcgcgcc aacgggtata atgactatcc accagtactc
     2641 acctccgact actgagacta acaaaacgcc cagccagggg agcataagat cagccaacca
     2701 acccgatccc atctggtgaa attgataatc tgccagatgc agcgcgatta tccagccaac
     2761 caggattaat aaaccgggta ctaaaaaaag acattgtcgc cagacggcta tttctctttt
     2821 ttctggtgca ctatcgcctg cccatactcg ccccgagaaa aatagcatta atgcgcccag
     2881 cgccagcata aaaatgttga gcagaaggta acttgttggc gtgataagga tattacaaac
     2941 ttcaataata ccggcaagtc cgacacccag catagcaaca accgccgcca aaaattgcgt
     3001 cagtatgggg atgccgaaaa aagtcattac cagcgaggac aaaatccatt ttctgttttg
     3061 cattattctt tccattcttt ttgaatggtg aaattatact ccccgagtcc ccttgcccct
     3121 tctggacact tttccgaaat gatggcggaa aaaaacg
//
LOCUS       DAJVTY010000253         3067 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVTY010000253 DAJVTY010000000
VERSION     DAJVTY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.255"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1010)
                     /locus_tag="OQQ66_003987"
     CDS             complement(<1..1010)
                     /locus_tag="OQQ66_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1906706.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCQLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NG"
     gene            complement(1272..2862)
                     /locus_tag="OQQ66_003988"
                     /pseudo
     CDS             complement(1272..2862)
                     /locus_tag="OQQ66_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT          983 a          565 c          480 g         1039 t
ORIGIN      
        1 ttaccattgc aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc
       61 attatgcgtt ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg
      121 ccggtttggt cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa
      181 ggctgaagac tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata
      241 ttagtgtcaa gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct
      301 atagtaagca actggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta
      361 gcaaaaagcg cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc
      421 ctggtacttg tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg
      481 gggtttataa ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc
      541 cagatgggtt tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata
      601 ttattaagta aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg
      661 taatttttac tattaattgt cacaataaac tgataattat tatccctacg tagaatatga
      721 gcacgcatgc ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa
      781 attttgtcat cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc
      841 acaacctcac cgagcattgt cctggcaata cttgatatat catcacctat accatttgca
      901 atatacccaa acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt
      961 gcgattgtcg ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc
     1021 atattattca ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt
     1081 tcgatggaaa aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat
     1141 ttcttatttc atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct
     1201 ctacaatcga ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca
     1261 ctcatggagt tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca
     1321 cgttcgttag gagctatctt ccaggaattt agccccatgg tataattatc aattttttga
     1381 tgctgatcta cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt
     1441 ccacatttga agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg
     1501 tcatatacag gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc
     1561 atgtgttgtt tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct
     1621 ttcatctgat taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt
     1681 cttattattc cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg
     1741 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     1801 gtatagttac cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt
     1861 atacgactat ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat
     1921 ttatcaccaa aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg
     1981 gtaatcgcat cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc
     2041 gtatctaata aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg
     2101 tcttcgtgtt ttaataattc acataataaa ctccccccct tttccctgat acctatatgt
     2161 cctgaaacga gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa
     2221 gccgtgttgg gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa
     2281 aacacagaat taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc
     2341 caacgctagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     2401 gaaaggccat taactttata aattatgtct gatttatctc cggagaaact aataaaataa
     2461 taaccatcct tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta
     2521 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat
     2581 tcttgaatat ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact
     2641 atattcattt accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc
     2701 gttatctatt tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt
     2761 tttatcaacc ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg
     2821 gcttgaagag atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt
     2881 ataacttgtt agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca
     2941 aagtaataat acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta
     3001 aataatggaa aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta
     3061 atcttag
//
LOCUS       DAJVTY010000254         3006 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVTY010000254 DAJVTY010000000
VERSION     DAJVTY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.256"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..2875
                     /locus_tag="OQQ66_003989"
     rRNA            <1..2875
                     /locus_tag="OQQ66_003989"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          794 a          662 c          937 g          613 t
ORIGIN      
        1 gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga
       61 tatgaaccat tataaccggc gatttccgaa tggggaaacc cagtgtgatt cgtcacacta
      121 tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca tctaagtacc
      181 ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg ggagcagccc
      241 agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg atacagggtg
      301 acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg ggacacgtgg
      361 tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga ctgaccgata
      421 gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt gaaaaagaac
      481 ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg cgtacctttt
      541 gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg gagccgaagg
      601 gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc cggtgatcta
      661 gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga ctaatgttga
      721 aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg agatagctgg
      781 ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg tagagcactg
      841 tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat accggagaat
      901 gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga aacaacccag
      961 accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga aggcccagac
     1021 agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag ctcactggtc
     1081 gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct gcggcagcga
     1141 cgctgatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt gctgtgaggt
     1201 atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg ggtgaaaagc
     1261 ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg tgagtcgacc
     1321 cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc tgtacttggt
     1381 gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg tcccggttta
     1441 agcgtgtagg ctggttttcc aggcaaatcc ggaaaactaa ggctgaggcg tgatgacgag
     1501 gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta agcatcaggt
     1561 aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc aaggcgcttg
     1621 agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag aaggcacgct
     1681 gatatgtagg tgaagtccct cgcggatgga gctgaaatca gtcgaagata ccagctggct
     1741 gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta tacggtgtga
     1801 cgcctgcccg gtgccggaag gttaattgat ggggtcagcg caagcgaagc tcttgatcga
     1861 agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
     1921 gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac ccgagactca
     1981 gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa agaccccgtg
     2041 aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata ggtgggaggc
     2101 tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc tttaatgttt
     2161 gatgttctaa cgttgacccg taatccgggt tgcggacagt gtctggtggg tagtttgact
     2221 ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat cctggtcgga
     2281 catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac ggcgcgagca
     2341 ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat cgctcaacgg
     2401 ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat cgacggcggt
     2461 gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc caagggtatg
     2521 gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag acagttcggt
     2581 ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg agaggaccgg
     2641 agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg tagctaaatg
     2701 cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat gagttctccc
     2761 tgactccttg agagtcctga aggaacgttg aagacgacga cgttgatagg ccgggtgtgt
     2821 aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta accttacaac
     2881 gccgaaggtg ttttggcgga tgagagaaag attttcagcc tgatacagat taaatcagaa
     2941 cgcagaagcg gtctgataaa acagaatttg cctggcggcc gtagcgcggt ggtcccacct
     3001 gacccc
//
LOCUS       DAJVTY010000255         2964 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVTY010000255 DAJVTY010000000
VERSION     DAJVTY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.257"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            189..929
                     /locus_tag="OQQ66_003990"
     CDS             189..929
                     /locus_tag="OQQ66_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1906707.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(900..1667)
                     /locus_tag="OQQ66_003991"
     CDS             complement(900..1667)
                     /locus_tag="OQQ66_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1906708.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1873..2451)
                     /gene="lpcA"
                     /locus_tag="OQQ66_003992"
     CDS             complement(1873..2451)
                     /gene="lpcA"
                     /locus_tag="OQQ66_003992"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1906709.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2691..>2964
                     /gene="fadE"
                     /locus_tag="OQQ66_003993"
     CDS             2691..>2964
                     /gene="fadE"
                     /locus_tag="OQQ66_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1906710.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAI"
BASE COUNT          713 a          748 c          747 g          756 t
ORIGIN      
        1 ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac
       61 cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag
      121 actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa
      181 taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt
      241 ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc
      301 agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatctct
      361 acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt aaatattccg
      421 gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg
      481 tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat attggtttcc
      541 ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc cacggcgatt
      601 gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg
      661 ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac ccgttccgca
      721 tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc tgggagcaac
      781 tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca
      841 atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca caactggcat
      901 caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat cttttgccag
      961 gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc
     1021 tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc aaacggtgcg
     1081 cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg cccatccgaa
     1141 agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc gatatattta
     1201 aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc
     1261 cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc ggtttccagt
     1321 gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg
     1381 cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg gcgaatatga
     1441 gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta
     1501 aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc
     1561 cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag
     1621 cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac
     1681 aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag gtcggatgcg
     1741 gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg ataagacgcg
     1801 tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg
     1861 cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac
     1921 tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc gaatttcgat
     1981 atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca ctttcatccc
     2041 cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat
     2101 ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt
     2161 gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg
     2221 gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc
     2281 gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc
     2341 taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc
     2401 cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca tgaggatatc
     2461 cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt
     2521 acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat
     2581 gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca tccactacaa
     2641 ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt
     2701 tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat
     2761 ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct ggtctgtggt
     2821 cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt gcgcctatgc
     2881 gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg ccgccgatgt
     2941 cgcgcactga gaaagaagcg attg
//
LOCUS       DAJVTY010000256         2910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVTY010000256 DAJVTY010000000
VERSION     DAJVTY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.258"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..230
                     /locus_tag="OQQ66_003994"
     CDS             <1..230
                     /locus_tag="OQQ66_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1906711.1"
                     /translation="RAGKISGILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQV
                     LIYDRKVKVSPTLDVPLPENIPSNSTCDFCH"
     gene            complement(258..347)
                     /locus_tag="OQQ66_003995"
     tRNA            complement(258..347)
                     /locus_tag="OQQ66_003995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Ser,seq:cga)
     gene            441..1238
                     /gene="mtfA"
                     /locus_tag="OQQ66_003996"
     CDS             441..1238
                     /gene="mtfA"
                     /locus_tag="OQQ66_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1906712.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1339..1414
                     /locus_tag="OQQ66_003997"
     tRNA            1339..1414
                     /locus_tag="OQQ66_003997"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1372..1374,aa:Asn,seq:gtt)
     gene            1728..2910
                     /locus_tag="OQQ66_003998"
                     /pseudo
     CDS             1728..2910
                     /locus_tag="OQQ66_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT          753 a          689 c          687 g          781 t
ORIGIN      
        1 atcgcgcagg caaaatttca ggtattttaa ccgacttcac ctttcatgat ctgaaggcga
       61 aaggaatttc agactatgaa ggaagcagtc gggataagca acttttctct ggtcacaaaa
      121 ccgaggggca agtgctaatc tatgacagga aggttaaagt ttcaccgaca cttgatgtcc
      181 cgttacctga aaatattcca agtaattcca cgtgtgattt ttgtcactga cttaatgatg
      241 tataagtgat tgaattttgg cggagagagg gggatttgaa cccccggtgg agttgccccc
      301 actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt cagatggttg
      361 ccatgatgcc aggaaatttg gcattttaac agtccctgtc cgtgcaattt tgttcaagtg
      421 acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa tcagcacatc
      481 aaactgccct tccctggcag gaagcactat cgatccccct tttaacgtgt ctgacggaac
      541 aggaacaaag caaattagtc actcttgccg aacgtttttt acagcaaaag cggcttgttc
      601 ctttacaggg ctttgaactg gattcattaa gaagctgccg gatagcactt ctattttgcc
      661 tacccgttct ggagttagga ctggaatggc tggatggttt tcatgaagtc ttaatttatc
      721 ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg cataaccaac
      781 gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac tggttggata
      841 tacaagattc ttttgatgct tctggtttta acctgattat tcatgaagtc gctcataagc
      901 tggacacccg taacggcgat cgcgccagcg gagttccctt tattccgttg cgtgaggttg
      961 ctggctggga acacgatctt catgctgcaa tgaacaacat tcaggaagaa atcgaattgg
     1021 ttggcgagaa tgcggcgagc attgatgctt atgcagccag cgatcctgct gaatgttttg
     1081 ccgtactttc tgaatatttc tttagcgccc cagaactttt tgctcctcgt ttcccttcat
     1141 tgtggcaacg tttctgccaa ttttatcaac aagatccttt gcagagactg catcacgcta
     1201 atgatacaga ctcgttttcg gcgacgaatg ttcattaatt aacaactttg catattaatt
     1261 aaccaattga aatgacttat gaaatttagt gttgacagac aaggtaccgc taagtaatat
     1321 gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
     1381 tatgtcactg gttcgagtcc agtcagagga gccaaattct aaaaattcgc ttttttagcg
     1441 caatgtcact gaccttagtt gaacattgtt ttttaacgga tagcgggttt ttaacatctt
     1501 aagcgccctc gacctttatg gttgagggcg ttttgctatg aacgccatca ccattttccc
     1561 ctcgattata aaacttgagt tattcagtag tctcccctct tgcaactcac acccaaaact
     1621 gcctaacgaa aagttattaa ttttcaatca tattgctatc agtatttaca ttttttcgct
     1681 gtgctagaaa gggcgcattt atgttagctc gttcagggaa ggtaagcatg gctacgaaga
     1741 agagaagtgg agaagaaata aatgaccgac aaatattatg cgggatggga attaaactac
     1801 gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc gttccctatg gctgcggcag
     1861 cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc tgcacaaatt gccattgcaa
     1921 atgccaatac ggtgccctac atccttggag cgctggaatc ggcccaaagc gttgccgaac
     1981 gtttcggtat ttcggtggct gagttacgca aactcaacca gtttcgtacg tttgctcgag
     2041 gttttaataa tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt agtgaaaaaa
     2101 aattaacccc gccgccgggt aatagcagtg acaacctcga gcaacagata gccagtactt
     2161 cacagcaaat cgggtctctg ctcgccgaag atatgaacag cgagcaagcg gcaaatatgg
     2221 cgcgtggatg ggcctcttct caggcttcag gcgcaatgac agactggtta agccgcttcg
     2281 gtaccgcaag aatcacgctg ggcgtggatg aagattttag cctgaagaac tcccagttcg
     2341 attttctcca tccgtggtat gaaacgcctg ataatctctt tttcagtcag catactctcc
     2401 atcgtactga cgagcgtacg cagattaaca acggcttagg ttggcgtcat ttcactccca
     2461 catggatgtc aggcatcaac ttctttttcg accacgatct tagccgttac cactcccgcg
     2521 ccggcattgg cgcggagtac tggcgcgact atctaaaatt aagcagtaac ggctatttgc
     2581 gactgaccaa ctggcgcagc gcacctgaac tggacaacga ttatgaagca cgcccggcca
     2641 atggctggga tgtacgcgca gaaagctggc tacccgcctg gccgcacctt ggcggtaaac
     2701 tggtctatga acagtattat ggcgatgaag tggccctgtt cgataaagac gatcggcaaa
     2761 gtaatcctca tgccataacc gctggactta actatacccc cttcccgctg atgaccttca
     2821 gcgcggagca acgctagggt aaacagggcg aaaatgacac ccgttttgcc gtcgatttta
     2881 cctggcaacc tggcagcgca atgcagaaac
//
LOCUS       DAJVTY010000257         2910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVTY010000257 DAJVTY010000000
VERSION     DAJVTY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.259"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1314
                     /locus_tag="OQQ66_003999"
     CDS             <1..1314
                     /locus_tag="OQQ66_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1906713.1"
                     /translation="VVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGP
                     GLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIF
                     FMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPH
                     IDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAM
                     GMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAP
                     LKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLYKWL
                     LNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACL
                     TGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1315..2223)
                     /locus_tag="OQQ66_004000"
     CDS             complement(1315..2223)
                     /locus_tag="OQQ66_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1906714.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2323..>2910
                     /locus_tag="OQQ66_004001"
     CDS             2323..>2910
                     /locus_tag="OQQ66_004001"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCS1906715.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          738 a          620 c          723 g          829 t
ORIGIN      
        1 gttgtttttt catgggtgtt tatctttgcg ctctatttca caccttatgc gctaattgtt
       61 ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg gattaaacac
      121 acgatgggac ccggactggc ttatctcgcc gcgtggacct actgggtggt gcatattccc
      181 tatctggcac aaaaacccca ggcaattctg attgcgctcg gttgggcgat gaaaggcgac
      241 ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct ggtgctgttt
      301 atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt cggttctgtg
      361 gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt aaccgcgcct
      421 gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt cattcctcat
      481 atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg cggagcagag
      541 aagatttctc cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc aaaagggatg
      601 ttatgcctgg cggtgatggt tgcggtttgt gccattctgg gctcactggc gatggggatg
      661 atgtttgact cgcgtaatat cccggatgac ttaatgacta acggtcaata ttacgccttt
      721 cagaagctgg gcgagtatta caacatgggt aatactttaa tggtgattta cgccattgcg
      781 aataccctgg gacaagtagc tgcgctggta ttctcgattg atgccccgct taaagtgcta
      841 ttaggtgatg ctgacagcaa atatattcca gccagtttat gtcgtaccaa cgcttctggt
      901 acgcccgtta atggctattt tctgaccctg gtgcaggtgg caattctgat tatgctgccg
      961 actctcggca tcggtgatat gaacaatctc tataaatggc tgttgaatct taactcggta
     1021 gtgatgccgc tgcgttattt atgggtattt gttgcattta ttgcagtcgt tcgcctggcg
     1081 cagaaatata aaccagaata tgtctttatt cgtaataagc ctctggcaat gaccgtcggg
     1141 atttggtgtt ttgcctttac cgcctttgcc tgtttgacgg ggatcttccc gaaaatggaa
     1201 gccttcactg cagagtggac cttccagttg gcgctgaatg ttgcaacgcc gtttgtgctg
     1261 gtaggattag gactgatttt cccgctgctg gcgcgtaaag cgaatagtaa ataattattg
     1321 tggtcggtga cgctgactct ggctgatctg acccgttttt acataatcga gaaatgcgcg
     1381 caagccgcag gacatatact ggcggtttgg ataatagatc tggaagcctg gacgctctgt
     1441 gctccagtct cccagtacac attctaaacg accagtatca aggtactcct tgatctgttc
     1501 atagagcaga tacccgatac cagcacccat caaaacggcc tccagttcag catcaacatc
     1561 atccagaatg atatttcctg cgacagcaat ttccagctcc tttgcaaatt gccagtggaa
     1621 aggtttgccg ctgggataac gaaaaacaac acattgatga ttaagcagat catgaggatg
     1681 gcggggtttg ccatatcttg caaagtactc tggcgttgct gctacacata atttcactgg
     1741 cgggccgatc gccacgctga tcatatcttt ttcgacgatg ccactaagtc gaacgccagc
     1801 gtcaaagccc tgttggacga tatcgacaag agagtcgtca gttgtgagct cgactttaat
     1861 atcaggatat tcacgtgtga aaccaaccag taaaggcatt aaaaatatac gtgcagctac
     1921 ccgggcgtca ttgattttga gcgttcctgt cggtgtcaga cgaaaatcat tcatctcatc
     1981 taacatgatc tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat tagaacctgc
     2041 ttccgttaac gatacgcttc gggttgtccg attgaataag cgaattttaa gacgttgttc
     2101 cagtgtttta atactatggc taatggcgga cgaggataag cctaactcat cgcccgctgc
     2161 acgaaagctt tgattacggg ccacggcaaa gaaagtggca aagtcagaca tattcatccg
     2221 cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacagg attatgccgc
     2281 ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt
     2341 tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc tgatgcgatc
     2401 catcagcggg caatggagcg gaaaatacag gtgacggaac tcgatttata tcggcgtaat
     2461 ttcaacccag tgatgacgcc ggaagatgaa ccagactgga agaatatgga taaacgttat
     2521 tctccagagg ttcatcagct ttattcagag ctgcttgaac atgacacgtt agtggtggtt
     2581 tttcctctct ggtggtacag cttcccggca atgctaaaag gatatattga cagagtatgg
     2641 aataatgggc tggcttatgg agatgggcac aaattaccat tcaataaagt tcgttgggtg
     2701 gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa aaatataagc
     2761 gattatttga aaaatatgtg cagttatctt ggtattgaag atgccgatgt cactttcttg
     2821 tgtaatacag tggtattcga tggggaagaa cttcatgcga gctattatca gtcgttatta
     2881 tctcaggtac gggatatggt agatgcacta
//
LOCUS       DAJVTY010000258         2832 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVTY010000258 DAJVTY010000000
VERSION     DAJVTY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.261"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..157
                     /locus_tag="OQQ66_004002"
                     /pseudo
     CDS             1..157
                     /locus_tag="OQQ66_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
     gene            complement(510..1250)
                     /gene="phoN2"
                     /locus_tag="OQQ66_004003"
     CDS             complement(510..1250)
                     /gene="phoN2"
                     /locus_tag="OQQ66_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1906716.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(1581..2447)
                     /gene="ospB"
                     /locus_tag="OQQ66_004004"
     CDS             complement(1581..2447)
                     /gene="ospB"
                     /locus_tag="OQQ66_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1906717.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            complement(2697..2832)
                     /locus_tag="OQQ66_004005"
                     /pseudo
     CDS             complement(2697..2832)
                     /locus_tag="OQQ66_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          865 a          526 c          515 g          926 t
ORIGIN      
        1 tttatcaata atggattggt tgatgtaaac aaaaaatttc aaaaagcaaa tagtggggac
       61 actatgcttg acaatgcaat gaaaagcaaa gattcaaaaa tgattgaatt ttttattaaa
      121 aaatggagcg gtatcaggca aacgatttga gaggtgacct aaaatatatt taggttgtgc
      181 cctgaatctg aagttgtcat ttctgttata ttgatcgaca taggggatgg tgcagaccgt
      241 tcccctgcaa caccacattg catgtggtga acggaaccgc cccgagtttc ctagagaatg
      301 ttttatctgt gaactcaggt tgccagatca tcgtttccga tggcagcata ataagctttt
      361 tctgcttctg ccggagggat atgtcccagc ctttccagta atcccttaaa ttcaggggta
      421 aaacgtggtg tgcccataga ctcctcctat gctcatatag ggtagatttg tctatgggcc
      481 ctccatagcc tgatacaggc tgtccagctt tatggggtca gttcattggt aggagtattt
      541 aattcttcaa actctttttt ggcttcgcta aggcttttgg taaattcagg tgtattatga
      601 agtactgcaa caaccgatgc tcccattaaa cgcccagcct ctacatcgct ttgccaatgc
      661 gcaccgcaga tgacccgact ttctccaaac tcatatccac gtcgaagtat ttccgcttta
      721 cgttgaggat taatctccgc aagtatcagt gctactgccc aaccaaagga tgcatgacca
      781 gagggataag agccagtgat agccatgttc tcatctttat caggtgtaca ggttgcgtct
      841 ttataaataa cgaatggacg aactctttta taatattctt tggcgttacg cacggcgtaa
      901 tcatggctat cctgtagtac ttgtgttaac aactgatata agattggggt attttccctt
      961 gaaatatcca tgccataagc atctgaaaat gcaagaccaa aattctcgta gtatgcatct
     1021 tcgctagcga gacgtacacg attagcatcc cggagcgagc ggccgaattc ataatgagct
     1081 ttgtcagcct gaaatactac tgaatcctct gccggaggcg gcggaagtat tgacaaactg
     1141 tctggtgaag tttgttgagt gagaaaacct tctgccttca gagcatttgc tgaggggata
     1201 aaaatcatat ttgtagcaat acaaaaaaga agaaagtttt tggttttcat aatttatttc
     1261 cttagcataa ttaattgatg attttggtca gctgtaaaaa acgagctgtg ttacaaaaaa
     1321 cacaagtcat gcggaaaaat ttgaacagag tgatggaaaa acaaaaaata gtcttatatt
     1381 aaagtaataa cattattttt catattcacg aaaaataatg tagtaaaaga atgatgtttt
     1441 ttatctattt gttcgtatcg ttttgtctct gtattatgat gatttgtgat tttccctcat
     1501 agggaagcac ttttctacta aagaaaaaaa ataacttcat cttaaatgca aacaattaac
     1561 tataggttaa aaattttata ctaatccagt tctttattaa taataaaagt ctttttctga
     1621 gagcttcttt ttactgtatg ctccggatca gcaggaattg aagttgaacg ataatgtgag
     1681 tagggaaaaa gctcttgacc atagtgaact ccatatccat gatagccgga tatttttaga
     1741 ccatcactca atgactcatc gttttcaagg tgttttttta tctgctctag caaagattct
     1801 ttttccttaa aaaaaggcag agagttaagg atacaagaaa gactttcagc aggggcattg
     1861 ttaaaatttt taggagccac tttatcagcg gagccgcatg aagtaaaacg gatatctttc
     1921 acatttagaa taccacagtc tttcattctt gaaatgattg tttgtacatc aataagttcc
     1981 gaaccaaggc ctaattgatg agaaccagga ctaccgtgcc caagaatata aaccttatta
     2041 tactctttaa aatcagtatc gtttactgtt tttattttcg ctttttttac agaaaaataa
     2101 gcagcagcat tcaatgcgtc atcatcatta tttattacta ttggttcact ctgtggtagc
     2161 catgattcag ccatttttag aaaatcattt ttactttttt catttttaaa aacataatca
     2221 tataacatct tgttcttaaa tactcttggt atttgttgtc ccataataga tagaacatca
     2281 ttgctatcac aaaaaccttt ctctcctaaa aaaaccaatg ctgcttttgg gtgagtacat
     2341 gatgaatttt ttaccctttt tattatatgt gcaaatgcaa tatctgatat gctttcattc
     2401 tttttattga ctgttctaca gtatggtcta acaccatcta aattcatacc tacatgactc
     2461 catatattgt tttataaaca tcatagtaaa gggtgttata ttttcatata gtatgcaagc
     2521 ttctttgtat actatgattt aaaaaaacgg aacaaaaatg caattatatt ctggcattgt
     2581 ttaatgcggt ttgtcttttt tattcattat ttcaatgaaa gaatttcaac tacaaagtta
     2641 attgatccta cctacataat atagacacag tacctaaagt accgtggaga tcatccgggg
     2701 aagccccggg tctgatacca gtgcaggtag accgctatct tgttccggta atgttctgcc
     2761 gttctttacg gcatcacctc tagcagaaac atcgcgtaac tgcgccaagt atgatgaact
     2821 tctcttaccg ct
//
LOCUS       DAJVTY010000259         2793 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVTY010000259 DAJVTY010000000
VERSION     DAJVTY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.262"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(7..843)
                     /locus_tag="OQQ66_004006"
     CDS             complement(7..843)
                     /locus_tag="OQQ66_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1906718.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(843..1646)
                     /locus_tag="OQQ66_004007"
                     /note="aph(3'')-Ib"
     CDS             complement(843..1646)
                     /locus_tag="OQQ66_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1906719.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1707..2522)
                     /gene="sul2"
                     /locus_tag="OQQ66_004008"
     CDS             complement(1707..2522)
                     /gene="sul2"
                     /locus_tag="OQQ66_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1906720.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          670 a          792 c          769 g          562 t
ORIGIN      
        1 tgatatctag tatgacgtct gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg
       61 ttgctcctct tctccatccg cgttccaagc tgcggaaagg cacccataag cgtacgcctg
      121 gtcgagcagg cgacgcggat cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc
      181 aatgcgtcta ggatcgagac aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc
      241 ggcgccaaag cccacttcac cgaccagacc gacgggatct atcaccagcc agccgcgact
      301 ggagaacatg atgttttcat gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt
      361 gctcatcatt tgatcggcta taatcgccgc gtggacgtag tcagtttgac aacctgcgtt
      421 ttgatcatcg cgcgcccgct gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc
      481 agaaggcagg ggttcctcag atgcggcata cagcttcgcc attagttccg ctgcaatttc
      541 ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag agcattcgct ccccggcata
      601 ttcgagcaac atcagattgt tctcacgacc gagcaaccgg actgctcccc tcccattgcg
      661 ccataccaga tagtcggccc cgcgcagttc atcagcaatg tcttctatag gtttcaatcc
      721 cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt
      781 gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa
      841 catcaacccc aagtcagagg gtccaatcgc agatagaagg caaggcgttc gcggtcgggg
      901 gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc gctctgcttc atctggcgct
      961 gcccagttct cttcggcgtt agcaatcatg agtgccaaat cggcatagcg atctgctgtt
     1021 ccgagccgcc caaggtcgat cagacccgtg cattgaagag ttttagggtc caccatgaag
     1081 ttcggcatgc agggatcacc atggcaaaca accatatcgg tgcgctcttg gtcgagccgc
     1141 accggtagct ctcgttcgac acgagccaaa agatcgagct gcggcgtact cttgtcctcg
     1201 tccggtaaga agtcgggatt gacggcattg cgggacacca catcaacggc gcgtccgaac
     1261 attcgcgaca gcctgcgctc aaacggacat tgatcaaccg ataggctgtg aacagcgcca
     1321 agttgctgcc ccattgacgg ccacgctttg agcaaatccg ctccagacag atcagccgcc
     1381 ggtactcccg gaattgccgt tatcaccaag catgcaccct cctgttcctc ctgccagttg
     1441 atgacctcgg ggcaagccac acctcgacct ttgagccaaa tgaggcggtc acgctctcca
     1501 gcgagctcac cgcggcggga agcaggtgcg attttcgcga aggcatgccc gtcaccacgt
     1561 cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca accagtcaga atgcgattca
     1621 ccaaaaaaaa tattagttcg attcaatgga ggttccttca gttttctgat gaagcgcgaa
     1681 tatagagaaa tatcccgaat gtgcagttaa cgaattcttg cggtttcttt tagcgccgcc
     1741 aataccgcca gcccgtcgcg caaggggcgc ggctcgtgtg tgcggatgaa gtcagctcca
     1801 cctgcggcgg cggcaagctc tgcagcgagt gtcgcggccc cgacatcccc cggaccacgg
     1861 cctgtgagcg cgcgcagaaa ggatttgcgc gaaacagaca gaagcaccgg caaatcgaag
     1921 cgcagccgca attcatcgaa ccgcgccagc accgagagcg aggtttcggg agcagccccc
     1981 agaaaaaacc ccatgccggg atcaaggaca aggcggttgc gtttgatacc ggcacccgtc
     2041 agcgccgcga tgcgcgcgtc aaagaacgcc gcaatgtgat ccatgatgtc gccagcgggt
     2101 gcctcgcgcc gatctgcctg cccgtcttgc accgaatgca taacgacgag tttggcagat
     2161 gatttcgcca attgcggata gaacgcagcg tctggaaaac cgcgaatatc attgagatag
     2221 gccacaccac gcgacaaggc ataggcttgc gtcgcgggtt gataactgtc gagcgagacg
     2281 ggaatgccat ctgccttgag cgcgtccagc accggcgcga tacgctcgat ttctgtgtcg
     2341 gacgaaacag gcgcggcgtc ggggttgctg gatgccggac cgaggtcgat cacatctgcc
     2401 ccctcggcca tcagcttacg cgcctgcgca atggctgcgt ctggcgccag ataccggcct
     2461 ccatcggaga aactgtccga ggttatgttg acgatgccga aaatgatgag cgatttattc
     2521 atgggggctt ctataataat aataatcgag catgagtctc atacggatgc tcgggtcgaa
     2581 agggaatccc caggcgagta acctgtttgc ggtgatccat tagctgcagg agcagaatag
     2641 catacatctg gaagcaaagc caggaaagcg gcctatggag ctgtgcggca gcgctcagta
     2701 ggcaattttt caaaatattg ttaagccttt tctgagcatg gtatttttca tggtattacc
     2761 aattagcagg aaaataagcc attgaatata aaa
//
LOCUS       DAJVTY010000260         2774 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVTY010000260 DAJVTY010000000
VERSION     DAJVTY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.263"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(124..308)
                     /locus_tag="OQQ66_004009"
                     /pseudo
     CDS             complement(124..308)
                     /locus_tag="OQQ66_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(470..544)
                     /locus_tag="OQQ66_004010"
     tRNA            complement(470..544)
                     /locus_tag="OQQ66_004010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Arg,seq:cct)
     gene            complement(620..1552)
                     /locus_tag="OQQ66_004011"
     CDS             complement(620..1552)
                     /locus_tag="OQQ66_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1906721.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1846..2601
                     /gene="mlaA"
                     /locus_tag="OQQ66_004012"
     CDS             1846..2601
                     /gene="mlaA"
                     /locus_tag="OQQ66_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1906722.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          688 a          658 c          671 g          757 t
ORIGIN      
        1 cccccaaacg aatcggaaaa tcgatactgg aaaaactcta actcggtggc cagtttttgt
       61 tgaccacttc aaacccatct tttacggggt gaaggtagat ttcgtatgaa gggtatctgt
      121 tgtctcctgc aaagtcataa gcggatattt tcctacggtc agtatttttt tcttaccgtc
      181 aatcttgtag cgaagttgcc ataccttttc ccggatacag gaacataaag gtacaggcca
      241 ttaccatcga gaaggcggta tggtttttct ttcggctttg ctgcttcaat ctgcttcacg
      301 gtgagcatgg gtaaaaatcc ggtgggtaaa attattttat ccacttttta cccgtcatgg
      361 agtgcggctg tcaacgatct gacgcgaacc atgacgaacc gtgaatatac ggaaggcttg
      421 atattcaggg gattttgcgg actggtacgg atgggagcga actgataaat ggtgtcccct
      481 gcaggaatcg aacctgcaat tagcccttag gaggggctcg ttatatccat ttaactaaga
      541 ggacaatgcg gcatgagtat acccgctaat ggactgcgga gtaagtacac tgccgctcga
      601 ttgcttaaac cctcgccatt tatgctgggt ttttatcatt tttcttaatg ttttccgcac
      661 gttctgcttt ttggcgtgct tctgctttgc gcttgttgct catgtcgtta cggatctgtg
      721 catgactcat taacgcgaag ataaaggtgc cgccgcagat attccccgct aaagtaggta
      781 gtgcgaaggg ccaaatgaaa tcgctccagt gcagcgtgcc gttaaacacc agatagagga
      841 tttctacaga accgacaacg atgtgggtgg tgtcacccag tgcaataagc caggtcatca
      901 atataatcac cacaatcttt gccgcacccg ctgcaggaaa catccaaacc atagtggcga
      961 tcagccagcc ggaaatgatc gcgttggcaa acatctcgct gggggtgttc ttcatcacat
     1021 ccatgccgat tttgacaaat gcatcgtgag tttcttcatt gaagataggc atatattcaa
     1081 atgcccatgc agcaatacct gtcccgagaa tattacccag cagcacgacg ccccataatc
     1141 gcataagtaa gccgacgttg ctcattgtcg gtttttgcat gacgggtagt accgcagtca
     1201 cggtgttttc ggtaaataat tgctggcggg ccataatgac gataataaaa ccaaaggtat
     1261 aaccgagatt ctccaacaag aagctgcccg gcacgccttc cagttcgaca tgaaatatcc
     1321 cttttgccag taacgaagcg cccatcgaca gacccgccgc aatggctgac cacagtagtg
     1381 ccattgcgtc gcgttccagc tctttttcac catcctggcg gatatgttca tgaattgcca
     1441 tcgcccggga ggggagtcga tcttcatcta tttctatttt tttgccgcgc tctttttctt
     1501 cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt
     1561 ctcttgaatt agcacgtata gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag
     1621 ttactctgaa aatgtagaaa aggccgcgtt tgcctttttc tgtttctata gaatcaagta
     1681 gcctacaggg cggcgattac caggctatga tcaaatcagc aaatcagggc gtctggacat
     1741 cagttgacgt gctgttacaa tcgcccacac ctaaacaggc ggatacggta tcgttccgtc
     1801 atggatggca aactgcataa gccataaaaa aacagggaga catttatgaa gcttcgcctg
     1861 tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg cgagttccgg tacagatcag
     1921 caagggcgtt ctgacccgtt agaagggttc aaccgcacca tgtacaactt caacttcaat
     1981 gtattagacc cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa
     2041 ccggcgcgta acggtttgag caactttacc ggcaaccttg aagaacctgc ggtgatggtt
     2101 aactacttct tgcagggcga cccttatcag gggatggtcc actttacccg ctttttcctg
     2161 aacaccattt tggggatggg cggctttatt gatgttgcag ggatggcgaa cccgaaactg
     2221 caacggactg aacctcaccg cttcggtagt acgcttggtc attatggcgt gggttatggg
     2281 ccttacgttc agttaccgtt ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg
     2341 gcggatggtc tttacccggt tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg
     2401 acgcttgaag ggatcgaaac ccgcgctcag ctgctggatt ccgatggtct gctgcgtcag
     2461 tcgtccgatc cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct
     2521 aatggcggcg aactcaaacc gcaggaaaat ccgaacgcac aagcgattca ggatgattta
     2581 aaagatattg attctgaata agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctttt
     2641 ttgttgttat ccctcaatta ccacaagtaa aaggagataa accgacaatg acccggacgt
     2701 aatgatgtaa aaatactgat gttcgtcact gactatttcc gatattcagg cgttgaaaaa
     2761 tcaaaaaact ggaa
//
LOCUS       DAJVTY010000261         2731 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVTY010000261 DAJVTY010000000
VERSION     DAJVTY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.264"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..2182
                     /locus_tag="OQQ66_004013"
     CDS             <1..2182
                     /locus_tag="OQQ66_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCS1906723.1"
                     /translation="KGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLL
                     PVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHRTSLLDSL
                     EIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTVRGLLTKEWMKGNRVSRI
                     TRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEVV
                     LKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLTETD
                     HENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETMEPD
                     TTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEG
                     ISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQEP
                     EKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHKENA
                     GSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGA
                     IFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALL
                     QLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGK
                     AIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            2241..2444
                     /locus_tag="OQQ66_004014"
     CDS             2241..2444
                     /locus_tag="OQQ66_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCS1906724.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            2444..>2731
                     /locus_tag="OQQ66_004015"
     CDS             2444..>2731
                     /locus_tag="OQQ66_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1906725.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSSESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKSCLP"
BASE COUNT          835 a          661 c          649 g          586 t
ORIGIN      
        1 aaaaggcggg aaagatggca tcacaatgac atttatcggc ccttccccct ctgttcagga
       61 caaatcagcc agcactgata ataccaacat caacggcgaa gacatgactg agattgagga
      121 gagcatgctt ctacctgtct ccggtcagga actgcccatt cgttggcttg ctcaacacgg
      181 cagcgaaaaa ccagtaacgc acgtttcacg cgacgaactc caggcattac acattgcacg
      241 ggctgaagaa ctaccggctg ttactgccct ggctatttcg catagaacca gtctgctcga
      301 ctcgctggag attcgcgacc tccacaaact ggttcgtgac actgacaaag ttttccctaa
      361 tcctggtaat tcagacctgg gactaataac tgcttttttc gaagcatact tggacgctga
      421 ctacactgtt cggggcctgc tgacaaaaga gtggatgaaa ggaaatcgtg tttcacgcat
      481 cacccgcacg gcttccggtg ctaatgctgg tggtgggaac aaaaccgatc gcaatccgaa
      541 tttagtacac accctcgaca cactggatgt ggagattgca gcagccacac ttccgatgga
      601 ttttaatatt tatgaaattc cgggcagcgt ttatcgtcgc gcaaaagaag tagtcctgaa
      661 aaaagaaagt ccgttcaaag aatggtccgc agcacttcgt gcacccccgg gtattctgga
      721 ctattcccgc gccgctattt ttgcacttat ccgaagcgca caccctgaat tttatcacta
      781 cccgggacgc cttcaggggt atatcaacgc ctatttgacg gaaactgatc acgagaaccc
      841 cagcaaggaa actctcacag ctacccggca tacgccggaa aaagatatcc tggaagaaat
      901 taaccgcgag gtggttactg agcgtgaaac agaagaagaa aaaccacaac catctgacgc
      961 aatggcaggt gaacaggcaa caactgaaac aatggaaccg gatacaactg aacattgcca
     1021 gaacgcgcag tcgctggatg ctcagtcgca ggtgagttcc gctaaccaag taaaagtcac
     1081 cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg acgccgataa
     1141 gttacttgct gtatcgcgtg gtgaatttgt tgaggggatt agcgacccta atgatccgaa
     1201 atgggtcaag gggatccaga ctcgcgattc tgtgaaccag aaccagcatg aatcggaacg
     1261 gaacgaccaa aaagcggaac aaaacagccc aaatgcgtta caaaacgagc cagaaacgaa
     1321 acaatccgaa ccagtagcgc aacaggaacc ggaaaaagtc tgcaccgcct gcggtcagag
     1381 cggtggcggc aactaccctg attgtggcgc ggtgatggac gacgcaacat accagaaaac
     1441 attcgatgaa gagaatcagg ttgaagttca ggaaaatgat ccggaggaaa tggaaggcgc
     1501 tgaacatcca cacaaggaga atgctggcag cgctcaggac cacgccagcg atagtaaaac
     1561 tggcgagacg gcagatccct taattacggt gaatggtcat cacgaaatca catccaccag
     1621 caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc ctgatgcccc
     1681 gatcatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg gacccgaatt
     1741 tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcgggaca ccattaaatg
     1801 gtggcttaag caatcacgcg aagcgcaatc tgccattatg accgatgaaa tcccgttaga
     1861 tgatgcactg ttacaattgc gggaatttat cgacgaaaac tccggtgaat tttttgttca
     1921 ggtctgggga aatggagcca acttcgacaa cacgattttg cgccgttcat acgaacggca
     1981 ggggatcccc tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa tcgttgagct
     2041 ggggaaagcc atagacttcg atgccagaac ggctattcca ttcgaaggtg agcgccataa
     2101 tgcacttgat gacgctcgtt accaggcaaa atacgtttca gctatctggc aaaaactgat
     2161 cccgagtcag gctgattttt aatgttcaac cctaattgcc gctaaccgta tatagttagc
     2221 ggcggttatg agatatagct atgagcagct tatttttaac cgaagatgaa ttgctaatat
     2281 taacgggctg caaatatgca agccaccagc gaaaatggtt aatggaaaac gagcttccgt
     2341 tctataccaa tcgtagtggc aaaccgattg tcagccggga tctatttacc tgcaataaaa
     2401 ctttaccacc acgcgaggta gagccgaatt ttggtgcgat ctgatgggaa gacgaaggaa
     2461 aaatcctgag cacgaaaaat tacctccaaa tgtataccca aataaatata gttatgtatg
     2521 gaaaccaaca tccagcgaat ctgtaacact aaccgccatc aaggatggtt tagctgcttt
     2581 atggaaaaag tatgaggaaa ctgtaaataa tcgcgatcgt gcaatgacat tcggtcgctt
     2641 gtgggaaaaa ttcctcgcca gcgcctatta cagtgacctc agtccaagaa cacaaaaaga
     2701 ttatctgcaa catcaaaaaa gttgcttgcc g
//
LOCUS       DAJVTY010000262         2718 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVTY010000262 DAJVTY010000000
VERSION     DAJVTY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.265"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..746)
                     /gene="curA"
                     /locus_tag="OQQ66_004016"
     CDS             complement(<1..746)
                     /gene="curA"
                     /locus_tag="OQQ66_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1906726.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCG"
     gene            927..1445
                     /gene="mddA"
                     /locus_tag="OQQ66_004017"
     CDS             927..1445
                     /gene="mddA"
                     /locus_tag="OQQ66_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1906727.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1442..1891
                     /locus_tag="OQQ66_004018"
     CDS             1442..1891
                     /locus_tag="OQQ66_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1906728.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1892..2125)
                     /locus_tag="OQQ66_004019"
     CDS             complement(1892..2125)
                     /locus_tag="OQQ66_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1906729.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2211..2384)
                     /gene="ortT"
                     /locus_tag="OQQ66_004020"
     CDS             complement(2211..2384)
                     /gene="ortT"
                     /locus_tag="OQQ66_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1906730.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2579..2674
                     /gene="yncL"
                     /locus_tag="OQQ66_004021"
     CDS             2579..2674
                     /gene="yncL"
                     /locus_tag="OQQ66_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1906731.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          653 a          696 c          660 g          709 t
ORIGIN      
        1 aagccacaaa cgggaatgcg cgcagatgta ttaagtaatg gtagcactgc atcaaatacc
       61 ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt caccagttgt
      121 tcggcaaaat cacccgcgtg gtgatcaaga caaacatcga agcccagcac ctcgatagca
      181 tggcggcatt tttccgcgcc accggccccc cccaccaccc tgcaaccttt aagtttgccg
      241 atttgcccca ccgttgctcc cacaggacct gtcgccgcag ctaccaccaa cgtttcgccc
      301 tctttaggct gaccgatatc cagcagcccc atataagcgg taaagcctgg catccccagc
      361 acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag atcatcacca
      421 ctggatatgt catagtcctg ccatccactg tagcccagca cccagtcgcc aggctgataa
      481 tcaggatgat tcgactccac aacatggctc accgtgccgc cgaccatcac gccgccaata
      541 tcaacaggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata cgggtccagg
      601 gacaaataaa ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac atcatcttct
      661 tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag aacccaacgt
      721 cgattacgct gcttttgttg ccccataatg ctctcctgtg cttttggcat gaatccatca
      781 atagtagcag gctcacggga ttggttttgt ttgcagccgt cacatcccct ctgcaacacg
      841 caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt gataacatca
      901 tcatcattat tttatcgagg ccagacatgt ccatccgttt tgcccgcaaa gccgattgtg
      961 ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc tggaatgacc
     1021 aaacggttga tgcagataac cgcattgcct ggtttgaagc gcggaccata gcaggttatc
     1081 cggtgctggt aagtgaagaa gatggcgtag tgacgggata tgcctcgttt ggcgactggc
     1141 gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat cccgatcatc
     1201 agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg cgggatagcg
     1261 ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg catctgcacc
     1321 attcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa tttgggcgct
     1381 ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg gacgcgattg
     1441 gatgaatcag tcgctcaccc ttgctttttt gattgccgcc ggaattggtc tggtagtcca
     1501 gaacacgctg atggtgcgca tcacccagac ctcctctacc attctcatcg ccatgttgct
     1561 gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac aaggcatggc
     1621 ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacccg gcttgctggg
     1681 atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg cgacaactat
     1741 tgcagtactg gtcgccagcc agctaattgg cggtctgatg ctggatatct tccgcagtca
     1801 tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac ttgtggttgg
     1861 cgcctggttg gtagccagac gctcatttta atcagaggat ggtgccgcct ttggttaact
     1921 gttcgtggcg cgcttccata tcttctttat gcttgcgacc gtgatgagca atagcggtgc
     1981 gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca tcgctcaggg
     2041 cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca tccacgcgcg
     2101 cgatgacttc atccagatgc gacatacttt ctccttaacc acttgtgaaa tttatcgatt
     2161 catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat ttaaaacagt
     2221 gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag aaaggcgctt
     2281 aagaaacgga tgcgtttctt atcgtgagaa agaaaccagg tgataagaaa tgcgattgct
     2341 gccataaccg cataaaaaac aagcatatgt tgatagagag acatgtacgg gcgtccagcc
     2401 agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag ttttttcacc
     2461 agtttgagaa aaaatcatca aacagcaact tgcccttatc catcttcaga atgagtatta
     2521 ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg tggcaaaaat
     2581 gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg gtttgtttac
     2641 tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcac gcagcacgta
     2701 ctgtcagaga aatcaact
//
LOCUS       DAJVTY010000263         2676 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVTY010000263 DAJVTY010000000
VERSION     DAJVTY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.266"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            268..579
                     /locus_tag="OQQ66_004022"
     CDS             268..579
                     /locus_tag="OQQ66_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1906732.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            579..866
                     /locus_tag="OQQ66_004023"
     CDS             579..866
                     /locus_tag="OQQ66_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1906733.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1403..2590)
                     /locus_tag="OQQ66_004024"
     CDS             complement(1403..2590)
                     /locus_tag="OQQ66_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1906734.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
BASE COUNT          688 a          714 c          637 g          637 t
ORIGIN      
        1 ccccattata aggggtacga ataaagtaac gggggtaagg tgaaatctgt caatcagtac
       61 cggcttacgc cgggcttcgg cggttttact cctgtgccat atgcaacaac ggagtgccgc
      121 cttccatgcc gctaacgcgg catcaaaaaa tccctcttct ccagttgccc ccctccgaac
      181 tcagagtgta ttttcattta ttgatgaaat ttactgtttg cgcaattttc ctgttttagt
      241 gtattttgtc attctggagg ctataatatg tttaatgtga ttactcaccc ggccgcactg
      301 gatgaacttc tagaattacc agacaatctg cgtggtcgta tgaccagact gattgaacgg
      361 ctggaacagg aaggtaacaa aatgaaaatg cctcacagcc gtgtaattgg cggagggctt
      421 tttgaactga gagtcgggga taaaaatatc gcaaggacac tgtacgctta tgcaacgggt
      481 aacgaaatct atctgctgca tgcatttgtg aaaaaaacgc aaaagacacc aacgaaagct
      541 attgaaattg ccaggatgcg tctgaaggag atgaactgat gaaagtaaaa ggtgttccat
      601 tcagccaggt aaagcaaaaa ctactcagta ctccagaagc tgtcaaaggg tatgaggatg
      661 ctgacaaaga actggagatg gtggaaatgt tatacgaaat gagagaaaaa gcgggattaa
      721 caaagtcagc actggccgaa cgaatggggt tacgcccgtc agccatcagt cgcctggaaa
      781 gtaacccttt aggggccagc atgaaaactt tgacaaaata tgctcatgcc tgtggcgcaa
      841 ccatcgatat tcacgctgtc tattgagggg tccgcttgga attcggaaaa taaaccacgt
      901 taagactatt ttttagataa atgactttca ggaatcgcca agatggcggg ataacagcgg
      961 ttttggtgtc ctaaatcgta caattctgta catccacgca agggaatgta cggaagtaag
     1021 ccagacggtt atcggacggt cagaacattt cgtacagcct ttacgttcag tgagttagct
     1081 ggcctggacg taaccactcg ttagctcaat tctcttagcg tgggaaaatt tccgaattcc
     1141 aagcggaccc ctttatcctt gccccgctga atgaggaaac gctgttccgg gggattatgc
     1201 tgaacgtctt ccgttcatcg ataggaatta aaaccccaaa aagattaaaa aacaccacaa
     1261 aacggatgtt tcttcaacac cacttttgct catatgaacg gaaccgacga ttaaactgga
     1321 tggctctgat tgattcaggg tatgaatggc ggttttttgc tccgtttccc tcaaaatgga
     1381 cgctacttac cctctgcggc tctcagccgc accaccgcat ccgggccagc ggctcatgca
     1441 tcaggacctg ctctgccaga cggtagcccc gcttcagccc cgtaaaacgc atctgactcc
     1501 cgcacagcac gcacttcagc gggtcaacct tcagtaacct ctgatacatc cctctccagg
     1561 tgatttgcat cgccgttttt ctcactgtct ccgttatgat gtacaccact tcttccagta
     1621 accgccgttt cgccggactc agaaaaccgt agtacctcac catacggaac cccttatccg
     1681 ccacatgcca ggagaacctt tccatgaact catctccact catcaacagg tattcttccc
     1741 gttttgttcg gtgactgttg taacgcagtc cgatttcatc ctgaccggca taatgctcca
     1801 gacgactcac cggcaccggt ggctttttca ggtaagagcc aaagtacacc gccacatggg
     1861 tggcattatc catcacccgg gatacgttga cattccagcc acggcggtaa tgcgtgtcca
     1921 ggaagcgatt ccattcccgt ttactgcttc cttctgctgc cagcgcatcc ggcatcacca
     1981 ggtcagggta tttccgtgac agtaaccgtg ttatccggta gcgccacatg ctcatcacct
     2041 tacgggcgta aaaatgaaga tttttccagg tgtggcccga cgtcacacca ccggcagttg
     2101 tcgataaatg gatatgcgga tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga
     2161 atatcctcgg ctccacatct gcctgatggc agatttccag tatcacatcc gctgcaatgc
     2221 ggctcatctc tgccagcaac caccggttgt ggaacaccag ggaccagtac tggcagggta
     2281 acgtgaacac aatatgctgc catgggcagt cggggaccag gctcagcaga tactgtatcc
     2341 actgtgcgcc agccttcacc ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga
     2401 ccttttttgt atggcaacag tccggtgatg aacagcacca ctgtgtatac cccatcagtg
     2461 tggtcccgca cgccatgatt ttggtcaccg actcaatcac caccggacgt actgcccctt
     2521 ccggctgctt ctccagccag ttaagccagc ggtttccctg ctgaaagata tcggcaaaac
     2581 ggggaagcat cagaagggcg gggcgactcc gtccggccag tgaaccgtgc cacactccgg
     2641 gcagtacata ccgccggcgc tgataccgga aagaat
//
LOCUS       DAJVTY010000264         2668 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVTY010000264 DAJVTY010000000
VERSION     DAJVTY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.267"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            231..770
                     /locus_tag="OQQ66_004025"
     CDS             231..770
                     /locus_tag="OQQ66_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1906735.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="OQQ66_004026"
     CDS             complement(996..1319)
                     /locus_tag="OQQ66_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1906736.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1592..1786
                     /locus_tag="OQQ66_004027"
     CDS             1592..1786
                     /locus_tag="OQQ66_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1906737.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          862 a          657 c          564 g          585 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagca aaacagtcga aaagtggtac gcgatataat cacataacgc
     1561 agatgacaga cagaacggag aaaacgaaaa gatgaacaag cagcagcaag ccgtactcaa
     1621 catggcggga ttcataaaaa gccagagcct gacgctgctc gaaaaactgg acgcactcga
     1681 tgctgacgag caggccacca tgtgtgagaa actacacgaa ctcgcagaag aactccagaa
     1741 cagcatacag acacgctttg aagtagaaaa tagcacagag agataacgct caggacagga
     1801 tgtcgaacgg cacaacagag cgtaaggaga aaatgtcaca taaggcgctc ttccgcttcc
     1861 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtatc cgctcactca
     1921 aaggcggtaa tgcggttatc cacagaatca ggggataacg ccggaaagaa catgtgagca
     1981 aaaagccgaa ggcagggaca aaggccgcgc aggtggcgtt tttccatagg ctccgccccc
     2041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2221 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2341 cggtaagaca cgaattatcg ccactggcag cagccactga taaccgaatt agaggagtaa
     2401 gacttgaagt catgcgcctg ttaaggctaa actgaaagaa caagttttgg tgactgcgct
     2461 cctccaagcc agttacctcg gctcaaagag ttggtatctc tgagaacctt cgaaaaaccg
     2521 ccttgcaggg cggttttttc gtttacaggc agtagattac gcgcagaaaa aaaggatctc
     2581 aagaagaacc tttaattttt tttcacactt ggtatcctac tttaacccat aaaataaaca
     2641 cttacaccga cacgccctta aacaaaag
//
LOCUS       DAJVTY010000265         2655 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVTY010000265 DAJVTY010000000
VERSION     DAJVTY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.268"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(914..1585)
                     /gene="queE"
                     /locus_tag="OQQ66_004028"
     CDS             complement(914..1585)
                     /gene="queE"
                     /locus_tag="OQQ66_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1906738.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1724..1864
                     /locus_tag="OQQ66_004029"
     CDS             1724..1864
                     /locus_tag="OQQ66_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906739.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1878..2655
                     /locus_tag="OQQ66_004030"
                     /pseudo
     CDS             1878..2655
                     /locus_tag="OQQ66_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          829 a          540 c          473 g          813 t
ORIGIN      
        1 tcggtttatc cctgttttta caaggaatat taactacact cgatgtcttt agaataaaag
       61 acaatacgaa tcgcacttta tcaattttca cttaaagtat gaactggata tctttattca
      121 taagaactat tcatcaacag cgtatagagg cggttatgta aaaccactca ttagcctcaa
      181 aacaaagaca aacttccaga tttattactt aaccaatgaa taacaagctt aaaaaatata
      241 ctgttattct aaagaaaaaa ttaacaggca ttaattcaat attaaaaaaa taatagatta
      301 aaatttctta acgatttaag aatcatacaa ataacacttt gattaaattt aattttttgt
      361 atcgataatt gtgaacctct ctggcatgga gaactatttt gaacatgagg tgttacgtgg
      421 atatgttgct tattacaagt actgctaata taaaaacttg agaaagagat aacgggttat
      481 atggtggttt atccccgctg gcgcgggaaa ctcccagctc ttgcccgcct caaagtgttg
      541 taactcggtt tatccccgct ggcgcgggga actctgtaaa cataacctat tattaattaa
      601 tgatttttta agccagtaac aatctaccaa ctttacagta tcacacaaac aacacatcca
      661 ttatgttaaa gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata
      721 caaattaaaa tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc
      781 acagaatttg tagataatgc taatgattag ccctggatgg gtaaaatgga ggagttttca
      841 gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct
      901 tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat
      961 tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct
     1021 gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc caatagttca tcgagtgctt
     1081 caatatcgcg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg
     1141 acaacacgtc atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg
     1201 gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa ccgttctttt
     1261 cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca
     1321 catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca ctgctcgccg
     1381 ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat
     1441 cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct
     1501 gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga
     1561 acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt
     1621 gcagatcctg ccgcaacaat catgccttat taacattctg ttacatgcag atttaaagaa
     1681 aaatgcgaaa catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa
     1741 agaaaatgga tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt
     1801 agtttttaat gataacgagt ctaaaattac cgatgccgcc gtcaatttat ttatccagat
     1861 ttaatgagaa atttataatg cgctatttca tactaatgtt cactttcgta tgttcctttg
     1921 ttgcagccca accaactatt gtcccacaat tacagtaaca agttacggat cttacgagta
     1981 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
     2041 cccaagtgca aattgctgta ttaattgttc ccacaaccaa agacgagaca attgaacaat
     2101 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
     2161 tgatcattgt tgcctggtca gatcgtactg tccgcatcca ggtaggttat gggctggaag
     2221 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
     2281 aacaacaaaa atttgctcag ggattagagc tagctataaa cgctttgaat aaccaactca
     2341 cttcacaaca ccaatatccg actaatcctt cagaaagtga atcagcgtct tccagtgatc
     2401 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
     2461 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatctatc
     2521 ttttagattt attcctgttc tccgataaaa ttttttccat tgctgtattt tccttctttt
     2581 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatcag
     2641 gtaaaagtta tcatt
//
LOCUS       DAJVTY010000266         2646 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVTY010000266 DAJVTY010000000
VERSION     DAJVTY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.269"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..455
                     /locus_tag="OQQ66_004031"
                     /pseudo
     CDS             1..455
                     /locus_tag="OQQ66_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(501..917)
                     /gene="rraB"
                     /locus_tag="OQQ66_004032"
     CDS             complement(501..917)
                     /gene="rraB"
                     /locus_tag="OQQ66_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1906740.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1079..2083
                     /gene="argF"
                     /locus_tag="OQQ66_004033"
     CDS             1079..2083
                     /gene="argF"
                     /locus_tag="OQQ66_004033"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1906741.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2155..2637)
                     /locus_tag="OQQ66_004034"
     CDS             complement(2155..2637)
                     /locus_tag="OQQ66_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906742.1"
                     /translation="MIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILV
                     NDIFLKDFFMKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVNNNPGYV
                     VEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKKLGWNN
                     "
BASE COUNT          706 a          618 c          594 g          728 t
ORIGIN      
        1 caggataatc ctgctattgc ccgcgtcatt cgtcaggtat ccgccgaata cggtcttacc
       61 gctgataaag gctacaccgt cgccgatccg aatcttgacg aactgtatca agtgtatagt
      121 cagccgggtc atgcatattg ggtggttgag tacgagggtg aagtggtcgg cggcggtggg
      181 attgcgccat tagcaggtag tgaatcggac atttgcgaac tgcaaaagat gtattttctt
      241 ccggctattc gcggtaaagg gctggcgaaa aaactggcct taatggcgat ggagcaggca
      301 cgagagatgg gcttcaaacg ctgctatctg gaaacgaccg ctttttaaag gaagccattg
      361 cgctttatga gcatttgggc tttgagcata tcgactatgc acttggctgc acgggccatg
      421 ttgattgtga agtgcggatg ttgcgcgaac tctaaaaaaa tgcccgctag tgtgtgctat
      481 cgggcatttt aatcaattaa ttagtggcga accccgtcat cgtcttcatc gacaaaatct
      541 tcatcgtcgc cgtcttcgcc attaggatct tcaaaataag tgccccaacc gtcgtactca
      601 acgtcaaatt tctccgccaa tgtcatcagt tgttcaacct gggcatcgat cagatcggca
      661 ttcaacgcgc actcgctgag gatgtcgcag caaatcacga tatcaccatc ttcaacttcc
      721 agctcttctg gatcggtcac ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc
      781 agggtttcca gatcgtctgc ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg
      841 ctgccatctt ccagtaattc ttcaataatc aaacgagttt cttcacgctg ttcttccagt
      901 tgttccgggt ttgccatggc tcattcctct taatgtcctg ccgatactct tattgtcaca
      961 taccactgac attgcctcca cctttgtgac aaagatttat gcttcagact tgcaaatgaa
     1021 taatcatcca tataaattga attttaattc attgaggcgt tagccacagg agggatctat
     1081 gtccgggttt tatcataagc atttcctgaa attactcgat ttcacgccag ctgaactcaa
     1141 cagcctgctg cagttagccg cgaagctgaa agccgataag aaaagcggca aagaagaagc
     1201 caaactcact ggtaaaaaca tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg
     1261 ctctttcgaa gttgccgcat atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg
     1321 cagtcagatt ggtcataaag agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta
     1381 tgacggtatt cagtatcgcg gctatggtca ggagattgcc gaaacactgg cggaatacgc
     1441 tggcgtgccg gtatggaacg gcctgaccaa tgagtttcac cccacgcagt tgctggcgga
     1501 tctcctcacc atgcaggagc atttacccgg taaagcattc aacgaaatga cgctggtcta
     1561 tgcaggtgac gcacgtaaca acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg
     1621 tctggatttg cgtctggtcg cgccacaggc atgctggccg gaagctgcgc tggttacgga
     1681 atgccgcgcc ctggcacagc aaaatggcgg gaatattacg ctgacggaag atgtagccaa
     1741 aggagttgaa ggtgctgact ttatctatac cgatgtatgg gtgtcgatgg gggaagcaaa
     1801 agagaaatgg gcagaacgga ttgcattact gcgtgattat caggtgaaca gcaagatgat
     1861 gcagttgacc ggtaacccgg aggtcaaatt cctccactgc ctgcccgcgt ttcatgacga
     1921 ccagacgacg cttggtaaga aaatggcgga agagtttggc ctacatggcg gaatggaagt
     1981 gactgatgag gtcttcgaat ctgccgccag cattgttttt gatcaggcgg aaaaccgcat
     2041 gcataccatc aaagcggtga tggtcgcgac gctcagtaaa taattgatat cgcaataaag
     2101 atgccgggaa atacaaaaac atcccggcat cacattattt tatgcagtaa tagttcaatt
     2161 attccacccc aattttttta ttgctatacg taaatcttcc ggaggattta atttttctaa
     2221 ctgttcttta ttaatatatt cacatatatt tattttccca tcccggagga tttccatcat
     2281 tcttgttcta atttttaatt cattacccat cttttcttca aaaatctcaa cgacataacc
     2341 cggattgtta tttacaattt catttgtttt ttcttcatta ccataaacat atcctaactc
     2401 attaagatac agctgttcgc cctcatactt cttaagtaat tgatagaagt accacttcgc
     2461 aaaatcaggt tgcttcatga aaaaatcctt caaaaaaata tcatttacta aaatgctgac
     2521 aggcacttca ttaaatagca acttaacatc atcaaataac cttagtatta agcgtgattt
     2581 ttcttgattg cttaagttaa aatcattgac atatgcaacc aacttatccc aaatcaattc
     2641 ctgtaa
//
LOCUS       DAJVTY010000267         2645 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVTY010000267 DAJVTY010000000
VERSION     DAJVTY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.270"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            21..857
                     /locus_tag="OQQ66_004035"
     CDS             21..857
                     /locus_tag="OQQ66_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906743.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFMLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            826..1389
                     /locus_tag="OQQ66_004036"
     CDS             826..1389
                     /locus_tag="OQQ66_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1906744.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1418..1951)
                     /locus_tag="OQQ66_004037"
     CDS             complement(1418..1951)
                     /locus_tag="OQQ66_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1906745.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1954..2511)
                     /locus_tag="OQQ66_004038"
     CDS             complement(1954..2511)
                     /locus_tag="OQQ66_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906746.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          754 a          565 c          587 g          739 t
ORIGIN      
        1 taaacagggc tggtaacgcc gtgcaaaaaa atggcgatac cttgtccggt gggcttactt
       61 ttgaaaatga ctcaatcctt gcctggattc gaaatactga ctgggcgaag attggattta
      121 aaaatgatgc cgatggtgac actgattcat acatgtggtt tgaaacaggc gacaacggca
      181 atgaatattt caaatggaga agccgccaga gcaccacaac aaaagacctg atgacgctga
      241 aatgggatgc actaaatatt cttgttaatg ctgtcattaa tggcagtctt ggagttggtt
      301 cgacgaatgc gttaggtggt agctctattg ttcttggtga taatgacacc ggatttaaac
      361 agaatggaga cggtattctg gatgtttatg ctaatagtca gcgtgtattc cgctttcaga
      421 atggagtggc tattgctttt aaaaatattc aggccgggga tggtaaaaaa ttcatgctat
      481 ccagctccaa caactccaca aagaatgcaa cgtttaattt atggggtgct tcaacccgtc
      541 cagtagttgc agagttaggc aatgaggcag gatggcattt ctatagccag cgaaatacag
      601 ataactcggt aatattttct gttaacggtc agatacagcc cagcaactgg gggaattttg
      661 attcacgcta tgtaaaagat gttcgcctgg gtacgcgtgt tgttcaattg atggcgcgtg
      721 gtggtcgtta tgaaaaagcc ggacacgcaa ttaccggatt aagaatcatt ggtgaagtag
      781 atggcgatga tgaagccatc ttcagaccaa tacaaaaata catcaatggc acatggtata
      841 acgtcgcaca ggtgtaaatt atgcagcata ttaaaaaata ttaagtccgg aaatccaaaa
      901 acaaaagaac aatatcagct aacaaagaat tttgatgtta tctggttatg gtccgaagac
      961 ggtaaaaact ggtatgagga agtaaataac tttcaggacg acaccataaa gattgtatac
     1021 gacgaaaata atattattgt tgccataacc aaagatgccc caacgcttaa tcccgaaggc
     1081 tttagtgtcg ttgagattcc agatataaca gccaatcgtc gtgccgatga ttcagggaag
     1141 tggatgttta aggacggaac tgtggttaag cggatttata cagcagacga acagcaacaa
     1201 caggccgaat cacaaaaggt cgcgttgctt tctgaagctg aaagcgttat tcagccactg
     1261 gaacgcgctg tcaggctgaa tatggcgatg gatgaggaac gcacacgact ggagtcatgg
     1321 gaacgctaca gcgttctggt cagccgtgtg gatacagcaa atcccgaatg gccacaaaag
     1381 cctgagtaaa aattaaggcc caatatcggg ccttctctca ttctggttgt tcgggaaacg
     1441 ttactggcag gctggaggtg tctgtagatt cgactttctg cgcatagagc atccactcgg
     1501 ttaatttttg tttattctcg tcggaaatga tgcccagccg tagctgtgag tcccatagct
     1561 gggttttatc cctgacaagt tgcaacaggc tttgcttttc attttccgct tgttgcctct
     1621 gctcttcctc ggtataagtt cgctttatca ctacgccatc tttgaacaac catttccccg
     1681 aaatatcagc ccggcgattt gctgtaatat caggtaattc aacgacgctt gcgccttccg
     1741 gattaattgc tgaaacatcc ttttcaatac aaataataac gccgttatgg taatagacca
     1801 ttttcaaagt gtctggctgg aaattctttt gttcctcata ccagtttttt ccatcatctg
     1861 aataaagcca tttgatgtta aattgctttg ttagctggta ttgctctttt gttttagggt
     1921 tgccagcagt aatgtttttt aagtgcatca tcgttaaata ctccccgcgt tataccacgt
     1981 cccattaatg caatactgaa ttggccttgc ctgtgttgta tcaattaatt catcacggtt
     2041 tccgttaact gaacccgtaa cgacataacc tgacctgtca gaccagccgg gaccattcca
     2101 tgtctgaaca gatgacagac cgccaaggcg aatacctgta ataaaccttg agttacattc
     2161 tgcccgcgta tatgcaccaa catctcccgc tgaaggtttt cgtgtcgtgg tatagaaatc
     2221 tgaccaacct aattcaaccc cataaccatc acgagccgac ctgtaggaaa tgccaccatt
     2281 tttataattc acacggaatt gtacggcagg acagctacca gcatttatat taaagtgaag
     2341 aattaatgca gatgcaccac caatcagggc gttatatgca ccactatccc aattccatcc
     2401 aacggatgta tcaccattaa cagtattacc tgtttgtcgg agtgcaaatg cattgacatt
     2461 tttggcacta attgagatat cgctagaacc atcaaaacca actccagcaa tttttcgtgc
     2521 tgttttcagc tttgttgcag tagcggcatt gccggacagt tccccagaaa ggccaccgct
     2581 gaatgtttgt cgattggtcc aggtattcgc tgtactgagt aacggtattt tctccccgct
     2641 tgtgc
//
LOCUS       DAJVTY010000268         2641 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVTY010000268 DAJVTY010000000
VERSION     DAJVTY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.271"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1075)
                     /locus_tag="OQQ66_004039"
     CDS             complement(<1..1075)
                     /locus_tag="OQQ66_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1906747.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRASH"
     gene            complement(1565..1641)
                     /locus_tag="OQQ66_004040"
     tRNA            complement(1565..1641)
                     /locus_tag="OQQ66_004040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1605..1607),aa:Arg,seq:tct)
     gene            complement(1655..1731)
                     /locus_tag="OQQ66_004041"
     tRNA            complement(1655..1731)
                     /locus_tag="OQQ66_004041"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1695..1697),aa:Arg,seq:tcg)
     gene            complement(1739..1814)
                     /locus_tag="OQQ66_004042"
     tRNA            complement(1739..1814)
                     /locus_tag="OQQ66_004042"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Ile,seq:cat)
     gene            complement(1855..2620)
                     /locus_tag="OQQ66_004043"
                     /pseudo
     CDS             complement(1855..2620)
                     /locus_tag="OQQ66_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393199.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          588 a          783 c          640 g          630 t
ORIGIN      
        1 aatggctcgc cctgtcctgt gacgtccagg tggctgagct gtcacgccat ttcgaaccgt
       61 aataaccgat accccgaatg tccgggtctt cttccggttt gttcgtcggc acattcaccc
      121 cgttctcatc ggtcatgaac ggtacgaaat ggatattctt ttccgtttta tttttatagc
      181 tgccgtacac cgtctggtac gtggattcgt tcttctgctt ccagaaatac gtcgtgtccc
      241 cgcatatcca gggaacaccg ccagcagagc caccgacgca ctggcctgcc atatccgcca
      301 ggtctgcacg gaatttatca accagcgcac caaactgtgc ggcatgattt gccggcgtac
      361 cgccaaaatc aaattccccc tgcatccaca ccacggcaaa cagcacattt ttcgggttct
      421 tctccagtgc cgcttttgtt cgaccgataa ggtccttata cagcggcttg tccacacccc
      481 agcgggttga attctccgag gcaccactcg cgtcactgta tgtgccatca gctccggtgg
      541 tgaacgctga accaccacga cagcacggaa ccagcagaat gcccgcattc gccggtataa
      601 acggcagcag ttttttggcg atatgcagcc cctgccccac ggttccgtac tgcccctttg
      661 acaggtccgc tttcggatgg ttaagacggc tcatgtcctg cacatcatgc agacaatggt
      721 ccgccggaat gatgtcgtta tatttgcatg ctgcaccgcc cggtgtcacc gtactgcgac
      781 gcgccagttg cttaatacgc gggtccggac ggtcatatgt ccccggcagc ggaaggcctt
      841 caccatacga catgccgttt gactgccctg ccagaaccac aacaaagtaa tactccgggt
      901 cgctggtggc gctgattact gcaccttctg ctgcaatcgc ctgcatcaga gtataagggg
      961 ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt gtcagctttt
     1021 ccgcaagatc tgacggcgac gccgccctga caacatcata gtgtttaaaa gccatgaatc
     1081 ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc ggaagttaca
     1141 ggacaatggc agaagagaga cgacagcccg caattcgaaa aagaccgcgc agttgcgcag
     1201 agtgactact atggggtatt attcgccagc tgaaatatta cttcacgttt tattgtttat
     1261 tccttgccgc ccgcgtctcc cagcgcgggc tttttttgcc cacaagaaag cccctccaga
     1321 gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc ccggtgaatt
     1381 cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt gcccctccgc
     1441 acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca gacaacagtc
     1501 aaccgtctga attgtgagac atttaaaaaa aaagcccgca aaagcgagcc agggaaaata
     1561 agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct
     1621 gtccggctga gctaacaacg catgatgcag ataatggacc gccatcgggg acttgaaccc
     1681 cgcgcagcca gcttcgaagg ctggcgctct ttcctgatga gctaatggcg gtatgtgatg
     1741 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1801 tgagctaaag ggccgggagc cgcataataa cgacgcgtaa ttaattcttc aatatcatcc
     1861 gttctggctg actaaatcct gtacttcccg aaccgtctgc tcaaaacgtt cagtctccag
     1921 ctcaacgcca attgcacgac gcccgagcgc cagtgccgct ttcactgtcg aacccgaccc
     1981 catgaaaaaa tctgcaatca ggtcacccgg acgactgcta gcgctgatta tctgctgcag
     2041 catttctgcc ggtttttcgc acggatgttt cccgggatag aactgcaccg gtttatgcgt
     2101 ccacacatcg gtatacggca cctgcaccgt cacgccaaaa taccgccgca gatgtttata
     2161 ttcactcagc agctccgcat actgccggtt cagtgaggta tacgtatcca ccagctggtg
     2221 gtggagcttt tccagttcac cacgccgatg cttctcttct gccacccggg caaacagcgc
     2281 ctgtaatttc agataatcgc tttcattcgg caactgccac tgactggcac tgaaccagtg
     2341 cgacaccatg tttttctttc ctgtggcatc tgcaatctgt tttgccgtta tccccagggc
     2401 agctcgcgca tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgcact
     2461 gcccttcgcc tcatagccat catctttcgg gcggtacggc ccctgataat gttccgcgaa
     2521 cagaatgcgc tctgtggcgg ggaaatacgc cctgcaggct ttccttgttg catccgttcc
     2581 agcgtccgga cggcttcgct cagataatat ggttcagcac actgaagcgt tcacgcatca
     2641 t
//
LOCUS       DAJVTY010000269         2575 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVTY010000269 DAJVTY010000000
VERSION     DAJVTY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.272"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(464..655)
                     /locus_tag="OQQ66_004044"
     CDS             complement(464..655)
                     /locus_tag="OQQ66_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1906748.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            720..1043
                     /locus_tag="OQQ66_004045"
     CDS             720..1043
                     /locus_tag="OQQ66_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1906749.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVFLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            985..1743
                     /locus_tag="OQQ66_004046"
                     /pseudo
     CDS             985..1743
                     /locus_tag="OQQ66_004046"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1721..2251
                     /locus_tag="OQQ66_004047"
     CDS             1721..2251
                     /locus_tag="OQQ66_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1906750.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2258..2491
                     /locus_tag="OQQ66_004048"
     CDS             2258..2491
                     /locus_tag="OQQ66_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1906751.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
BASE COUNT          639 a          597 c          822 g          517 t
ORIGIN      
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccatcggc
       61 gcggccttct tcctgttcct ggtcttttgc tcacatgttc tttccggcgt tatcccctga
      121 ttctgtggat aacagcattt cgtttttgag tgagtagata ccgctcgccg cagtcgaacg
      181 accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgt gcattttctc
      241 cttacgcatc tgtgcggcat ttcacacccg gtatggcgca cttttcatac aatccgcact
      301 gatgccgcat ggtgaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
      361 cgccccgaca cccgctaaaa cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      421 ccgctcacag ataagctgtg accgtctccg ggagctgcat gcgtcagagg ttttcaccgt
      481 catccccgaa gcgagcgagg caactgcggt aaagctcatc ggcgtggtcg tgaagcgatt
      541 cacagatgtc tgcctgttca tccgcgtcca gttcgttgag cttctccagc agcgttaatg
      601 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta gtcactgatg
      661 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga
      721 tgctcacgat acgggttact gatgatgaac atgctcgttt gctggaacgt tgtgagggta
      781 aacaactggc ggtatggatg cggcgggtct tcctggggga gccggttgcc cgttccggga
      841 ggctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctga
      901 atcagacagc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
      961 tggccgcact gatggccatc ggggatgagc tgcgccggct gcgtctggcc gtcagggaac
     1021 aggggacgcg ggatgatagt taagtttcat gccaggggaa aaggtggtgg cagtggtccg
     1081 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat
     1141 ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtccggt
     1201 gttctctcgt tcgcggagaa ggcgctgcca gcgggaggac gtgaaaaagt gatggcgagt
     1261 tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct gtgggtggag
     1321 caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga gctacagacc
     1381 ggaaaacgcc tccagccgta ctacgaccgg gcagacaggt cgagaattga tgcctggcag
     1441 acgctggtga atcaccatta cgggctgcat gacccgaatg ccccggagaa ccgccggacg
     1501 ctgactctcc ctgataacct gccggaaacg aaacaggcgc ttgctgagag cattacacgg
     1561 gggatagacg ctctttatca tgtcggggag ataaaagacc gtcaggatgt gattcaggcg
     1621 ctcacagagg cggggcttga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac
     1681 ccgaacggcg ggaagaatat cagactgaaa ggagcattct atgagcaatc ttttacagac
     1741 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa
     1801 cgacgagttc aggaagctcg aagaatctgt aagcaaggct gtgacatcaa acgagacgaa
     1861 aatcaaagac gctatagccc tgttcacagc ttcgacagag gaatcactga aaaaacaccg
     1921 ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc tggcagggaa
     1981 tacgggcatg atgttgctgg ggatggttat tttcctgttt accgtgagtg gcgggacgct
     2041 ctggtatctt ggcgggatga tacaggcgaa cctggaagaa atcaggaagc agaacgagag
     2101 tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgagggga gcaacggacg
     2161 ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg aaaacgggaa
     2221 acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg aaagacattt
     2281 gctgaacgca ttagagcaac tgcaacagga ctatatgcag cggctgaacg aatgggagag
     2341 cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa
     2401 cgagcaggtc atgcagttga gtcagcaggt gcagcacttg agcgagcgga cagaacgctt
     2461 gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca ggaatgcgaa
     2521 catgagttat ccctggagcg tgagcgccag ccggaaatac aggaacgcac actgg
//
LOCUS       DAJVTY010000270         2421 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVTY010000270 DAJVTY010000000
VERSION     DAJVTY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.274"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1552
                     /locus_tag="OQQ66_004049"
     CDS             <1..1552
                     /locus_tag="OQQ66_004049"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1906752.1"
                     /translation="EKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVPFQNGPTRG
                     KDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIR
                     RQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLLAIVKYHCTHR
                     GQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMIFGQGAIRC
                     HPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSSTPTGDATKR
                     YYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAVLKRYDDEG
                     RNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRHYLAPSDKL
                     DHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQRICKELGK
                     NLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELATKPVKLPE
                     KVRKVEAA"
     gene            complement(1595..2041)
                     /gene="ivy"
                     /locus_tag="OQQ66_004050"
     CDS             complement(1595..2041)
                     /gene="ivy"
                     /locus_tag="OQQ66_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1906753.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2222..>2421
                     /gene="yafV"
                     /locus_tag="OQQ66_004051"
     CDS             2222..>2421
                     /gene="yafV"
                     /locus_tag="OQQ66_004051"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1906754.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMT"
BASE COUNT          559 a          630 c          661 g          571 t
ORIGIN      
        1 ggaaaagctg ctgggtggcg cagaagattt aggtattacc tgtgcgctga tcccgaccac
       61 cacgccgggc gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg
      121 accgacgcgc ggtaaagatg tcttcgtacc gatcgattac atcatcggtg ggccgaaaat
      181 ggccgggcaa ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct
      241 gccttccaac tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca
      301 cattcgccgt cagttcaaaa tctctatcgg aaagatggaa gggattgaag agccgctggc
      361 gcgtattgcc ggtaatgcct acgtgatgga tgctgcggca tcgctgatta cctacggcat
      421 tatgctcggc gaaaaacctg ccgtgctgtt ggctatcgtt aagtatcact gtacccaccg
      481 cgggcagcag tcgattattg atgcgatgga tatcactggt ggcaaaggca ttatgctcgg
      541 gcaaagcaac ttcctggcgc gtgcttacca gggcgcaccg attgccatca ccgttgaagg
      601 ggctaacatt ctgacccgca gcatgatgat cttcggacaa ggagcgattc gttgccatcc
      661 gtacgtgctg gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact
      721 gttgttcaaa catatcggtc acgtcggtag caacaaagtt cgcagcttct ggctgggcct
      781 gacgcgcggt ttaaccagca gcacgccaac cggcgatgcc actaaacgct actatcagca
      841 cctgaaccgc ctgagcgcca acctcgccct gctttctgat gtctcgatgg cagtgctggg
      901 cggcagcctg aaacgtcgcg agcgcatctc ggcccgtctg ggggatattt taagccagct
      961 ctacctcgcc tctgccgtgc tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct
     1021 gccgctggtg cactggggcg tacaagatgc gctgtatcag gctgaacagg cgatggatga
     1081 tttactgcaa aacttcccga accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc
     1141 gaccggacgt cattatctgg caccttctga caagctggat cataaagtgg cgaagatttt
     1201 acaagtgccg aacgccaccc gttcccgcat tggtcgcggt cagtacctga cgccgagcga
     1261 gcataatccg gttggcttgc tggaagaggc gctggtggat gtgattgccg ccgacccaat
     1321 tcatcagcgg atctgtaaag agctgggtaa aaacctgccg tttacccgtc tggatgaact
     1381 ggcgcacaac gcgctggcga aggggctgat tgataaagat gaagccgcta ttctggtgaa
     1441 agctgaagaa agccgtctgc gcagtattaa cgttgatgac tttgatccgg aagagctggc
     1501 gacgaagccg gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt aattgaataa
     1561 cggagccgaa aggctccgtt tctttatccg ctaattattt aaaattaaag ccatccggat
     1621 ggttttccag gctgccggtc aacgccgcga acagcaccgt tttaccatca atcgaaagcg
     1681 catcgttcac attcagccag gtgagtttct cttgcgacgt tttctcatca atagtcgaga
     1741 acagccccgt catctgatta gatttctcgg accacatcac agcgatacgt tgcgagccac
     1801 agtcatgcgg tttgcacgcg ctcatcacct gatacgtctc atctcccaac gttacggttt
     1861 gtgcgggagt ataagtaccg cctttcatca cccaggcagg cagcttatgc ccttgtacca
     1921 tctgattaaa tgcagctttg gtggtttcgc cctttgcaag gctgctaatg gttaaatcat
     1981 cctgcgccat tgcactggtg gcgatgacca gagcggcgac tgtcgttatt gccttaaaca
     2041 tcattcctcc cgagcttatc ctgcccatgt tattaacctt ctaaagcctg gcgataacac
     2101 gataatttgc aaatcagata tttccaatat cagcatttgt tatcaattta ttctgcgcta
     2161 aggcaactgc ctgataaggt gttaaagtta taaaaccctt cgcataaagg agttgtacac
     2221 cgtgcctggt ttgaagatta cgcttttgca gcaaccactg gtgtggatgg atggccctgc
     2281 caacctgcgt cattttgatc gtcaactgga aggtattacc gggcgcgatg tgatcgttct
     2341 accggagatg tttaccagcg gctttgccat ggaagcggca gcttcgtcgc tagcacaaga
     2401 tgacgtagtg aactggatga c
//
LOCUS       DAJVTY010000271         2349 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVTY010000271 DAJVTY010000000
VERSION     DAJVTY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.275"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..706)
                     /gene="folP"
                     /locus_tag="OQQ66_004052"
     CDS             complement(<1..706)
                     /gene="folP"
                     /locus_tag="OQQ66_004052"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1906755.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAEL"
     gene            complement(700..1047)
                     /locus_tag="OQQ66_004053"
     CDS             complement(700..1047)
                     /locus_tag="OQQ66_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCS1906756.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(1277..1750)
                     /locus_tag="OQQ66_004054"
     CDS             complement(1277..1750)
                     /locus_tag="OQQ66_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA7"
                     /protein_id="HCS1906757.1"
                     /translation="MKISLISATSENGVIGNGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSRKGISSSNENVLVFPSIEIALQELSKITDHLYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDINFPKIPENFNLVFEQFFLSNINYTYQIWKKG"
     gene            1908..>2349
                     /gene="intI1"
                     /locus_tag="OQQ66_004055"
     CDS             1908..>2349
                     /gene="intI1"
                     /locus_tag="OQQ66_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1906758.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRI"
BASE COUNT          594 a          613 c          597 g          545 t
ORIGIN      
        1 gaagttccgc cgcaaggctc gctggaccca gatcctttac aggaaggcca acggtggcgc
       61 ccaagaagga tttccgcgac accgagacca atagcggaag ccccaacgcc gacttcagct
      121 tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc ggggctcaag aaaaatccca
      181 tccccggatc gaggatgagc cggtcggcag cgaccccgct ccgtcgcaag gcggaaaccc
      241 gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc gggtcgaagg tgaccggtgc
      301 gggtggcgat gccatcccgc tgcgctgagt gcataaccac cagcctgcag tccgcctcag
      361 caatatcggg atagagcgca gggtcaggaa atccttggat atcgttcagg tagcccacgc
      421 cgcgcttgag cgcatagcgc tgggtttccg gttggaagct gtcgattgaa acacggtgca
      481 tctgatcgga cagggcgtct aagagcggcg caatacgtct gatctcatcg gccggcgata
      541 caggcctcgc gtccggatgg ctggcggccg gtccgacatc cacgacgtct gatccgactc
      601 gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc tagccgccgg ctctcatcga
      661 agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt caccatggcg tcggcctccg
      721 cagcgacttc cacgatgggg atcgggcgag caaaaaggca gcaattatga gccccatacc
      781 tacaaagccc cacgcatcaa gcttttgccc atgaagcaac caggcaatgg ctgtaattat
      841 gacgacgccg agtcccgacc agactgcata agcaacaccg acagggatgg atttcagaac
      901 cagagaaaga aaataaaatg cgatgccata accgattatg acaacggcgg aaggggcaag
      961 cttagtaaag ccctcgctag attttaatgc ggatgttgcg attacttcgc caactattgc
     1021 gataacaaga aaaagccagc ctttcatgat atatctccca atttgtgtag ggcttattat
     1081 gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt
     1141 gcttagtgca tctaacgccg agttcagcgg cagtttttaa gttgtggttt tatggaatac
     1201 ttttgcgcag caaaaccata aaaccgcgac ttaaaaactg tccaaggagc gcagcgactg
     1261 gtgctggaac gacttgttag ccttttttcc aaatctgata tgtgtaattt atattagaca
     1321 aaaaaaactg ctcaaaaacc aaattgaaat tctctggaat tttaggaaaa ttgatatcac
     1381 cttcaacctc aacgtgaaca gtagacaaat gaattatatc tgctttttca ataagactat
     1441 tgtagatttg accgccacca gagacatata aatgatctgt aattttcgat agttcttgca
     1501 aagcgatttc tattgaagga aagactaata cattttcatt tgagcttgaa attcctttcc
     1561 tcgacactac tgcatatttt cgatttggaa gaacacccat agagtcaaat gttttccttc
     1621 caacaaggag ccactgatta tatgtgagcg ctttaaagag taactgctca ccttttgctg
     1681 accatgggat atcagggcca ttaccgatta cgccattttc tgacgttgca gaaatcaatg
     1741 aaattttcaa ttcaaccccc gtaatggcta actttgtttt agggcgactg ccctgctgcg
     1801 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     1861 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     1921 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     1981 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
     2041 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     2101 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
     2161 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
     2221 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
     2281 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca gcttctgtat ggaacgggca
     2341 tgcggatca
//
LOCUS       DAJVTY010000272         2298 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVTY010000272 DAJVTY010000000
VERSION     DAJVTY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.276"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            701..2209
                     /gene="ltrA"
                     /locus_tag="OQQ66_004056"
     CDS             701..2209
                     /gene="ltrA"
                     /locus_tag="OQQ66_004056"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1906759.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVISTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          582 a          567 c          677 g          472 t
ORIGIN      
        1 ccagctgtgg gtggctgatt ttacttacgg tgtgcccgga gttcagggcg ggcatggatg
       61 cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat gagaaaccca
      121 acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg gcatgcccgc
      181 agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc gagatgatcc
      241 tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct ctgtgggttg
      301 aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg gcagtgatac
      361 gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg cagcagcgtc
      421 tatcacgctt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa agagtgcgac
      481 gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt cagcgacgga
      541 tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta gtagtccgcg
      601 atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc gacgtgaatt
      661 aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca agcttgccac
      721 atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctaa taacacaacc
      781 agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc atacccccgg
      841 cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc tgcaaatcct
      901 cagggatgaa ttactctcag gccactacca gcccttgccc gcaagacggg tttacatccc
      961 taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc gtattgttca
     1021 gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata cgctctcata
     1081 tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat tacagctcac
     1141 agactgcggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca gttacttcga
     1201 caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg acgcacgttt
     1261 catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc tctttcgggc
     1321 ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga acatcatgct
     1381 gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca gaaaagatcg
     1441 gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag aaaactggca
     1501 gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca tcgtcaaagg
     1561 caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg aaggcagtct
     1621 gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg gctttatctt
     1681 tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag tgatctcaac
     1741 gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt tatcaggcaa
     1801 ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga aaggctgggc
     1861 catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg accgtgtcgt
     1921 gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg cttccctgat
     1981 gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt tatttggtaa
     2041 aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc aaggcaagaa
     2101 gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga ccagaaacac
     2161 gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa tggagagccg
     2221 gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata gtccgactac
     2281 gccctgcctc ttactcta
//
LOCUS       DAJVTY010000273         2292 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVTY010000273 DAJVTY010000000
VERSION     DAJVTY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.277"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OQQ66_004057"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OQQ66_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1906760.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OQQ66_004058"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OQQ66_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OQQ66_004059"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OQQ66_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1906761.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OQQ66_004060"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OQQ66_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1906762.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          612 a          487 c          542 g          651 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agcaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaggg caacagatcg cgtacccggt
     1021 ttgccggcca gtcttggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattca ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatacgataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aaccacggca gcctggcggc
     2161 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagttccca
     2221 tgtcacacaa cctggagcac cagaaagtcc acaccagaat ggtgaaagag gttctgaaag
     2281 ccgtcgccag ag
//
LOCUS       DAJVTY010000274         2278 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVTY010000274 DAJVTY010000000
VERSION     DAJVTY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.278"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            368..2020
                     /locus_tag="OQQ66_004061"
     CDS             368..2020
                     /locus_tag="OQQ66_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1906763.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFTR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2007..2207)
                     /locus_tag="OQQ66_004062"
     CDS             complement(2007..2207)
                     /locus_tag="OQQ66_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906764.1"
                     /translation="MTKKLAALQHQLSRKVKFSNNRQKQKHKIQQLHSHIANIRRDYF
                     HKVTATVSKKHVMIVNYVITAN"
BASE COUNT          706 a          421 c          534 g          617 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtcggtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 cacccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 ggggcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtga gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gcctgttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatattt
//
LOCUS       DAJVTY010000275         2175 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVTY010000275 DAJVTY010000000
VERSION     DAJVTY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.279"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            376..519
                     /locus_tag="OQQ66_004063"
                     /pseudo
     CDS             376..519
                     /locus_tag="OQQ66_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..934
                     /locus_tag="OQQ66_004064"
                     /pseudo
     CDS             666..934
                     /locus_tag="OQQ66_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1327..>2175
                     /locus_tag="OQQ66_004065"
     CDS             1327..>2175
                     /locus_tag="OQQ66_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1906765.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          728 a          463 c          364 g          620 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagtatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattaa
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaatatat cagggtttgt tactaataac ttctgaaaac agaaaaaaaa
     1021 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
     1081 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtacag ggagagacgc
     1141 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
     1201 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
     1261 ttaaaattaa taagctatat tctatttcac tagaattctt atcgctgata agaaatttta
     1321 gttataatga ttaaatcaac caatatacag gcaatcggtt ctggtattat gcatcaaata
     1381 aacaatatat actcgttaac tccatttcct ttacctatgg aactgactcc atcttgtaat
     1441 gaattttatt taaaagcctg gagtgaatgg gaaaagaacg gtaccccagg cgagcaacgc
     1501 aatatcgcct tcaataggct gaaaatatgt ttacaaaatc aagaggcaga attaaattta
     1561 tctgagttag atttaaaaac attaccagat ttaccgcctc agataacaac actggaaata
     1621 agaaaaaacc tattaacaca tctccctgat ttaccaccaa tgcttaaggt aatacatgct
     1681 caatttaatc aactggaaag tttacctgcc ttacccgaga cgttagaaga gcttaatgcg
     1741 ggtgataaca agataaaaga attaccattt cttcctgaaa atctaactca tttacgggtt
     1801 cataataacc gattgcatat tctgccacta ttgccaccgg aactaaaatt actggtagtt
     1861 tctggaaaca gattagacag cattcccccc tttccagata agcttgaagg gctggctatg
     1921 gctaataatt ttatagaaca actaccggaa ttacctttta gtatgaacag ggctgtgcta
     1981 atgaataata atctgacaac acttccggaa agtgtcctga gattagctca gaatgccttc
     2041 gtaaatgttg caggtaatcc actgtctggc cataccatgc gtacactaca acaaataacc
     2101 accggaccag attattctgg tcctcgaata tttttctcta tgggaaattc tgccacaatt
     2161 tccgctccag aacac
//
LOCUS       DAJVTY010000276         2127 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVTY010000276 DAJVTY010000000
VERSION     DAJVTY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.280"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(3..485)
                     /locus_tag="OQQ66_004066"
     CDS             complement(3..485)
                     /locus_tag="OQQ66_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1906766.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OQQ66_004067"
     CDS             complement(476..778)
                     /locus_tag="OQQ66_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1906767.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="OQQ66_004068"
     CDS             1050..1322
                     /locus_tag="OQQ66_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906768.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            1303..>2127
                     /locus_tag="OQQ66_004069"
     CDS             1303..>2127
                     /locus_tag="OQQ66_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1906769.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIRVEAVTKML
                     ACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGG"
BASE COUNT          572 a          543 c          533 g          479 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctgga tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaacacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcacagcca accagtgctg gacgtccttc
     1381 ctggatgcgg gcggtctgcg cgatatcagg gttgaggctg tcacaaaaat gctggcctgc
     1441 ggcacgcgga tactgggagt gaaggaatac atctgcgata aacctgagtg cccccacgtc
     1501 agatacgtca ctaactcatg cggcagccgt gcctgcccgt cctgcggaaa aaaggccaca
     1561 gacctgtgga tagcgacaca gctgaatcgt cttcctgact gcgactgggt acacctggtc
     1621 ttcaccctgc cggacacgct gtggccggtg ttcgaaagca accggtggct gctgaatgac
     1681 gtgtgccgtc tggcggtgga gaatctgctg tatgccgccc ggaaacgggg gcaggaaccc
     1741 ggtatcttct gcgccatcca cacgtatggc cgtcgtctca actggcatcc gcatgtacat
     1801 gtgtctgtaa cctgtggagg tctgaataag catggtcagt ggaaaaagct gagcttcctg
     1861 aaagacgcga tgcgttcacg gtggatgtgg aatatgcggc agctgcttct gaaagcgtgg
     1921 tcagaggggc tggcgatgcc ggagtcgttg tcacatatca cgacggaatc acagtggaga
     1981 agcctggtgc tgaaagccgg aggaaaatac tggcatgtgt acatgtcgaa aaaaacggcc
     2041 ggcgggagga atacggcgcg ctacctgggt cgttatctga agaagccgcc gatagcggcc
     2101 tcccggctgg cacattacaa cggaggg
//
LOCUS       DAJVTY010000277         2112 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVTY010000277 DAJVTY010000000
VERSION     DAJVTY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.281"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(35..286)
                     /locus_tag="OQQ66_004070"
     CDS             complement(35..286)
                     /locus_tag="OQQ66_004070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906770.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            209..898
                     /locus_tag="OQQ66_004071"
                     /pseudo
     CDS             209..898
                     /locus_tag="OQQ66_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(915..1094)
                     /locus_tag="OQQ66_004072"
                     /pseudo
     CDS             complement(915..1094)
                     /locus_tag="OQQ66_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1424..>2112
                     /gene="ipaH7.8"
                     /locus_tag="OQQ66_004073"
     CDS             1424..>2112
                     /gene="ipaH7.8"
                     /locus_tag="OQQ66_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1906771.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
BASE COUNT          630 a          470 c          389 g          623 t
ORIGIN      
        1 ctggcaaaag tgtagcggta ttaagcgcag ctatttagat tattgccgca ttttctccac
       61 cacgatcaga gccgaaaaaa gataattttt ttccaactgc cacccggcgt aatgcgtttt
      121 caccaatgtt accccaccta ttgcggcaaa aggtaatgtc ttcaatcgat agaagtttct
      181 ggtaatctac tggtatttga ggttttccat gactaccgtt actgttcatt gtccgcgctg
      241 taattccgat gaagtttatc gacatggccg tagttgttcc cggcatgagc gtttccgttg
      301 tcgctcatgt aaacgtgttt tccagctcac ttactcttat gaagccagaa aactcggcgt
      361 taaggagcag atcgttgaaa tggcacataa cggcgcaggg ggctgttata ccgccagaac
      421 actgaaaatc ggcatcaata ccgtcatacg gacattaaaa agctctcgcc ccggcggata
      481 acatctgcgc cagtcgctca cgctgatgtt gcccttatct gtgaacttga tgagcagtgg
      541 cgcttcgttg gcagcaaggc gcaacaacac tggccctggt atgcgtacaa cactaaaact
      601 ggggtgtgtt ggcttacact tgcgggccac ggaatgatga gacctgtcgg gaactgttag
      661 ccctgttgac tccgttctgt atcggcatgg tgaccagcga tgactggggc agctacgcca
      721 gagaagtgcc ggaggagaaa cacctgaccg gcaaaatctt cacgcaacgt atgaacgtaa
      781 caacctgacc ctccgccccc gcatcaagcg gctggcacgt aaaacaatct gcttctcgcg
      841 ttccgtggaa atccacgaaa aagtcatcgg ttccttcatt gaaaagcaca tattctaatg
      901 attggaagta ctacccggca aaactcattc agtgcattcc agtgattcag gatatagttg
      961 aacgccttta ccatttcagc atggtttgac aaagttttcc tctggagctt tatccagtca
     1021 tacaaggact tcattcgcgg cacacatttc tcctgcctta ccgcaagccg tttttctgcc
     1081 ggcctccctc gtattaaatg tgtatcgttt ttttacagta attttaattt atcattctat
     1141 aataggaata aatgagcttg gctaaaatat gatataaaac aaatatactt actcggatta
     1201 catttccttg ctcactggaa tgttgaattc agtgttatta ttgactgcag tcacaataaa
     1261 aaaaagaatt ccatagaaaa cctcaacaaa taaattacaa ctaacttctg ttatgtgtaa
     1321 aatggaaact attaaaactt aatatcgaaa atggtaagtg aaatttgtat aaatatacaa
     1381 ttttaaatat ttattctcac aaatataagg ttgccctagc attatgttct ctgtaaataa
     1441 tacacactca tcagtttctt gctccccctc tattaactca aactcaacca gtaatgaaca
     1501 ttatctgaga atcctgactg aatgggaaaa gaactcttct cccggggaag agcgaggcat
     1561 tgcttttaac agactctccc agtgctttca gaatcaagaa gcagtattaa atttatcaga
     1621 cctaaatttg acgtctcttc ccgaattacc aaagcatatt tctgctttga ttgtagaaaa
     1681 taataaatta acatcattgc caaagctgcc tgcatttctc aaagaactta atgctgataa
     1741 taacaggctt tctgtgatac cagaacttcc tgagtcatta acaactttaa gtgttcgttc
     1801 taatcaactg gaaaaccttc ctgttttgcc aaaccattta acatcattat ttgttgaaaa
     1861 taacaggcta tataatttac cggctcttcc cgaaaaattg aaatttttac atgtttatta
     1921 taacaggctg acaacattac ccgacttacc ggataaactg gaaattctct gtgctcagcg
     1981 caataatctg gttacttttc ctcaattttc tgatagaaac aatatcagac aaaaggaata
     2041 ttattttcat tttaatcaga taaccactct tccggagagt ttttcacaat tagattcaag
     2101 ttacaggatt aa
//
LOCUS       DAJVTY010000278         2032 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVTY010000278 DAJVTY010000000
VERSION     DAJVTY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.282"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..560)
                     /gene="capU"
                     /locus_tag="OQQ66_004074"
     CDS             complement(<1..560)
                     /gene="capU"
                     /locus_tag="OQQ66_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1906772.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPV"
     gene            complement(563..1405)
                     /locus_tag="OQQ66_004075"
     CDS             complement(563..1405)
                     /locus_tag="OQQ66_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1906773.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            complement(1769..1885)
                     /locus_tag="OQQ66_004076"
     CDS             complement(1769..1885)
                     /locus_tag="OQQ66_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1906774.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
BASE COUNT          601 a          462 c          369 g          600 t
ORIGIN      
        1 acggggcatc cccttcggga cgccagccaa gaaagaacat ttggaggcaa actgtttcgg
       61 gaatcaacgt ataatttctg gaaatcaaag cctggaggca caacagtaac ccgggttcga
      121 catccttcct gctccagatg tgtcttcata cttgttccgg gaacaatcac ctcatcgcaa
      181 aaatgattta ttgaaaaaac ttttgttttt tgtgtcaaat atgtcttttg cctgataatt
      241 ctgaatggat gtttccaagt gaataaccgt actaaaccaa caatattgct atcatgccca
      301 ctgtgacaaa cgattgcatt gggctgaaaa ccatgaacta ttccgagtaa tctccatgca
      361 gtagggatgt gaagactgtt tcgaaataac gcgaatgtga tatcaattcc caatttactg
      421 gcttcaaaag caattttgct gttttccctg cagacaagca gaactgaatg ccccattttc
      481 ttcagggcct tcatttgagc aacagcctgt aattcctgac cacctatatt cggtgatgat
      541 tccgtaaata gtatattcat tattaatcat ccggtaatct tggcccctta tgaaaggcca
      601 gcaacgatga aaaaaacggt gttgtataat aaaaaagtcg tcttttcagc catgcgcaac
      661 tttcccgttc tttggtgctt attcttccta tccgtgtagt tcctttagca ggagcattca
      721 ttcttctttc tgttgtatat aaataataga atccaagttc ttcagcgacc tgaatataat
      781 ccttattata ataaccttcc ggccaacaca aatgtttact gcattttcct gtcatttctt
      841 tcaaatattc tctcccactc aacaagtcct gccgaagatg tttacactgt tcttctcgtg
      901 aactgaactt tttatcccag cgtgtatgag tatgcgtatg aacatgaaat tcgaccagac
      961 cagattgcaa catttcgttt acttcagacc agcgaagcat aacattatca gcattccctg
     1021 tcgctatttg atgttcgcaa tcgcgatgag aatattcctt tccaggagag tgacgaaccg
     1081 gaccattccc aataaaaccg gtaattaaaa aaatatgtgc tttgagatta aactctttta
     1141 ataaaggata tacctgaaac cagttgtcaa ggtatccatc atcaaatgtc aacataacgc
     1201 ttttccgggg gagttttccg ccccgataaa aaaactccag ttcatctgat gacagtgttt
     1261 tccagttatt ttcagccaac catttcattt gcttccggaa tgtcacggga gaaagtgtaa
     1321 ccagtccggg gcaatgactg acatgatgat acattaatac cggaacatga ttggctttga
     1381 acaaaatgcc cccttcgttt aacatttaaa gtttctggca cctagtaagc agagacaaaa
     1441 ggaaaaccca ataaagctgg tgcattttat ttccagatac tggatgaata acatagcaaa
     1501 cccatggcat actcgatata ttttactaaa ttaaacatca acaattcata aacaatgata
     1561 tattatcata tcttttaaaa acgactcact aagaaccttg aggtggcaca ctgaatttgg
     1621 ctacctgaac agaggtgata tgttcacctc agaacaacac aggtgctcca atgaaaaaaa
     1681 ggaatttcag cgcagagttt atttacgggg tgacgctttc attaacctgt acacgaactt
     1741 ctattccggc atcatgacag gcctgcagct actgcgccat ttccagcgga tcgccctccc
     1801 ggcgtaccac cccaccttct ttattccata actgcagagc atttttctga ttgttccatc
     1861 tgatttcggg gggtattccg ggcaaggttg ttcagtaagg cattccttcg taaaatcagc
     1921 atgttttttg cacgacggaa accggcagcc atgaaaatct gtctttatca tactctgaat
     1981 cctgacacga tcccgggtta taaaaaattt gcacaggcta ttgccaccga ta
//
LOCUS       DAJVTY010000279         1981 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVTY010000279 DAJVTY010000000
VERSION     DAJVTY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.283"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            294..587
                     /locus_tag="OQQ66_004077"
     CDS             294..587
                     /locus_tag="OQQ66_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1906775.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            659..1318
                     /locus_tag="OQQ66_004078"
     CDS             659..1318
                     /locus_tag="OQQ66_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1906776.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1410..1856
                     /locus_tag="OQQ66_004079"
     CDS             1410..1856
                     /locus_tag="OQQ66_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1906777.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          521 a          424 c          519 g          517 t
ORIGIN      
        1 aggttagcag tgcaaacagc aatacagcct tactgatgct cttgattttc attggtagtc
       61 tcctgttcag aatgagtacg atgaactgtt ataatataac aatccctaac gggagaattc
      121 cagtggcttt tctgcgttcg gtaaaatccc ggcgcggcat tttttgccgg ggggattact
      181 tataaagggg catggactca acaaaccgca gaaatctaca gctggctggc ttgtggcgcg
      241 ggtttcattg atgttagctt atgccaaaac ctggcatctt aaagagtaaa agtatgtttt
      301 gtgtgattta tcgaagcagc aagcgtgacc agacctattt atatgtcgaa aaaaaagacg
      361 atttttcgcg tgttcctgag gaactgatga aaggttttgg tcagcctcag ttagcgatga
      421 ttctgccgtt ggatgggcgg aagaaactgg tcaatgctga tattgaaaag gttaaactgg
      481 cattaaccga gcaaggttac tatttgcagt taccgccacc acccgaagat ttgctgaagc
      541 aacatctttc cgtcatgggg cagaaaacag acgacacgaa caaataaccg atatccggcg
      601 gtggcattat ctttgtcggc gcgggttttc atatccacga taaggtgagg ggaacgttat
      661 gtatcaacat cacaactggc aaggtgcgct gctggattat ccggtgagta aagtagtctg
      721 tgttggcagt aactatgcca aacatattaa agagatgggc agcgcagtgc ccgaagagcc
      781 agtgctgttt attaaaccag aaacggcact gtgcgatctg cggcagccat tggcgatccc
      841 atccgatttc ggttcagttc atcatgaagt cgaactggcg gtgttgattg gtgcgacgct
      901 gcgtcaggca acggaagagc atgtccgcaa agccattgcc ggttacggcg tggcgcttga
      961 tctgacattg cgtgatgttc agggaaaaat gaagaaagcc gggcagccgt gggaaaaggc
     1021 taaagcgttt gataactctt gtccgctttc cgggtttatc cccgcggcgg aatttaccgg
     1081 cgatccacaa aatacaacgc tgggcctgag cgtaaacggc gaacaacgcc agcaaggtac
     1141 gactgcggac atgatccata aaatcgttcc gttgatcgct tatatgagca agttttttac
     1201 cctcaaggcc ggtgacgttg tgttgacagg cacgcctgat ggcgtcggcc cgttgcaaag
     1261 cggtgatgag ctgacagtca ctttcgatgg gcattctttg acaactcgcg ttttgtaata
     1321 ctttttgccg cctgaaagcg gcggcaaaac ttgcatcgct gtgccagact ggttataagg
     1381 tgcgttttaa cgtaatggcg gaacacctga tgaacgatgt acctttctgg caaagtaaaa
     1441 ccctggacga aatgagcgat gcggaatggg agtcgttgtg tgatggttgc ggtcagtgtt
     1501 gcctgcataa actgatggat gaagacaccg acgaaatcta cttcactaac gtcgcctgtc
     1561 gccagctcaa tattaaaacc tgtcaatgtc ggaactacga acgtcgtttc gagtttgaac
     1621 ccgactgcat taaattaacc cgtgaaaatc tgccaacatt cgaatggctg ccaatgacct
     1681 gcgcttatcg tttgctggcg gaaggtaaag atttacctgt gtggcatccg ctacttactg
     1741 gttcgaaagc ggcaatgcat ggtgaacgta tctctgtgcg tcatatcgca gtgaaagaat
     1801 cagaagtgat tgactggcag gatcatatct taaataaacc tgactgggcg cagtgataaa
     1861 tttaatagat gttgatgggg gctttcttaa tatacgtctg gatgctcgcc atttcgtagc
     1921 cctgtttttg aaagcgctct gtcataatga gagttacccg ataccttatt atcaacaggt
     1981 a
//
LOCUS       DAJVTY010000280         1950 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVTY010000280 DAJVTY010000000
VERSION     DAJVTY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.284"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..851
                     /gene="potA"
                     /locus_tag="OQQ66_004080"
     CDS             <1..851
                     /gene="potA"
                     /locus_tag="OQQ66_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1906778.1"
                     /translation="SYALFPHMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETF
                     AQRKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLG
                     ITFVFVTHDQEEALTMSDRIVVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNA
                     TVIERLDEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLI
                     GYVRERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVL
                     ADEEHK"
     gene            835..1698
                     /gene="potB"
                     /locus_tag="OQQ66_004081"
     CDS             835..1698
                     /gene="potB"
                     /locus_tag="OQQ66_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1906779.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
BASE COUNT          451 a          472 c          501 g          526 t
ORIGIN      
        1 aaagctacgc acttttcccc cacatgaccg tgttcgaaaa tgtggccttt gggttgcgca
       61 tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat ggaagccctg cggatggtgc
      121 aactggaaac cttcgctcaa cgtaaaccgc atcagctctc tggtggtcaa cagcaacgcg
      181 tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt gttgctggat gagtcgctct
      241 cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga gctgaaagcg ttacagcgta
      301 agcttggcat tacattcgtc ttcgtgactc acgaccagga agaagcactc accatgtcag
      361 acaggattgt ggtgatgcgc gagggtcgca ttgagcaaga cggcacgccg cgtgaaatct
      421 acgaagagcc gaaaaacctg tttgttgccg gcttcattgg cgaaattaat atgtttaacg
      481 ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat
      541 gtaatatcta cgttaacttc gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc
      601 cggaagactt acgtgttgaa gagattaacg acgacaacca cgctgaaggg ctgattggtt
      661 acgttcgtga gcgtaactac aaaggcatga cgctggagtc ggttgttgaa ctggaaaatg
      721 gcaagatggt gatggtcagc gaattcttca atgaagacga tcctgacttt gaccactctc
      781 tcgaccaaaa aatggccatt aattgggtag aaagctggga ggtcgtactg gctgatgaag
      841 aacacaagta agttccagaa tgtagtgatt gtcactattg tcggttggct tgtgttgttt
      901 gtctttctgc ccaacctgat gatcattggc accagctttt tgacccgcga cgacgccagt
      961 ttcgtcaaaa tggtctttac gctggataac tacacgcgtc tgctcgatcc gctctatttt
     1021 gaagtgctat tgcactcgct gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg
     1081 ggctatccat ttgcctggtt tctggcgaag ctgccacaca aggtgcgtcc gctgctgctg
     1141 tttctgctga ttgttccgtt ctggaccaac tcattgattc gtatctacgg gctgaaaatt
     1201 ttcctcagca ccaaaggcta tctcaacgag tttttgctct ggctgggcgt tatcgacaca
     1261 ccaatccgta tcatgttcac gcccagtgcg gtgattatcg gtctggttta cattctgctg
     1321 ccgtttatgg tgatgccgct gtactccagt atcgaaaaac tggataagcc gttgcttgag
     1381 gcggcgcgcg atctcggtgc cagcaagtta cagactttta tccgcataat tattccactg
     1441 acgatgccgg ggattattgc cggatgtctg ctggtgatgc tgccagcgat gggcctgttc
     1501 tatgtgtccg acctgatggg cggtgcgaaa aacctgctga tcggtaacgt catcaaggta
     1561 cagttcctca atattcgtga ctggccgttt ggtgcagcca ccagcattac gctgactatc
     1621 gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc gtttgctgaa taagaaggtg
     1681 agcgaattag atgattaatc atttatattc aatgcattaa gtttgttttt gagtggtttt
     1741 gccgtttaag gtaacggcgt tatagatcct atcaaaatca atcaattcca ttctggtttt
     1801 ggcgaaagat ttttcgagag cttcggtctt taactaaaac tgattgcgta taaccccaat
     1861 acccctcccc gactatctga attgtcgggg aggcacttca cgcacaaaat atcatccctg
     1921 cgccgttgat tactctgcag gtgaagtcgg
//
LOCUS       DAJVTY010000281         1948 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVTY010000281 DAJVTY010000000
VERSION     DAJVTY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.285"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>1948
                     /locus_tag="OQQ66_004082"
     CDS             <1..>1948
                     /locus_tag="OQQ66_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1906780.1"
                     /translation="KKKVSQKKITFTANAEQLLQPQFTVEQLRELGIKVDEIPALAEK
                     DDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARGYVSPSRWDDGIPTLFT
                     NYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTRNDQASSWNTISSYLQ
                     RDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQRGFAPTVRGIANSSA
                     IVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESDGTQRRFIQPYSSLPM
                     MQRPRHLKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFTLYGGLLGSEDYYALG
                     IGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDIPETNTNIAVSYYRYT
                     NDGYFSFDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYASGSQQDYWGNNEKNRNI
                     SVGVSGQQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERWLPRSRVSYQMTSQKDR
                     PTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGNDSS
                     QYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVIPY
                     LTTYQENRLSVDTTQLPDNVDLEQT"
BASE COUNT          541 a          475 c          447 g          485 t
ORIGIN      
        1 taaaaagaag gtttcacaga aaaaaattac atttaccgcc aatgcagagc aacttctgca
       61 gccacagttt acggtagaac aactacgtga gctgggtatt aaggtggatg aaatcccggc
      121 gctggctgaa aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac
      181 agctgctgaa tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact
      241 gtaccgtgat gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct
      301 gtttaccaac tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca
      361 acgacagtac ctgaatatgc aaaatggtgc taattttggc ccctggcgat tacgtaacta
      421 ttctacgtgg acacgcaacg atcaggcgtc aagctggaac actatcagta gttatttaca
      481 acgtgatatc aaggcgttga agtctcagtt gcttctggga gaaagcgcca ccagcggcag
      541 tattttttcc agctacacct ttactggcgt gcaactcgct tccgacgata atatgttgcc
      601 aaacagccag cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt
      661 gactatcagg caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga
      721 aattaacgat ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag
      781 tgacggtaca caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc
      841 taggcatcta aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag
      901 caaggaaccc gaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct
      961 gtatggcggc ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac
     1021 acttggcgca ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa
     1081 ccagcactct tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac
     1141 caacaccaat atcgctgtta gctactatcg ctataccaac gatggctatt ttagttttga
     1201 tgaagccaat acccgcaatt gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa
     1261 catcagccag acaatatttg atggggtaag tctgtatgcc tccggttcgc agcaagacta
     1321 ttggggcaat aacgagaaaa acaggaatat ctctgttggg gtttccggcc agcaatgggg
     1381 aattggttac agcctgaatt atcaatacag ccgctacact gatgaaaata atgaccgtgc
     1441 actctctttg aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta
     1501 tcagatgacc agccagaaag atcgcccaac ccaacatgaa atgcgtcttg atggctcact
     1561 gctggatgat ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca
     1621 taacagtagc ctgaacgcca gttaccgttc accttatgga accttcagtg ccggatacag
     1681 ttacggtaat gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatcc
     1741 tcatggcgtg acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaacgg
     1801 ggcttctggc gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc
     1861 agtgattcct tatctcacga cttatcagga aaaccgtctc tcggtagata ctacgcagct
     1921 gcccgataac gtcgatcttg aacaaaca
//
LOCUS       DAJVTY010000282         1917 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVTY010000282 DAJVTY010000000
VERSION     DAJVTY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.286"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1863
                     /gene="ppsA"
                     /locus_tag="OQQ66_004083"
     CDS             <1..1863
                     /gene="ppsA"
                     /locus_tag="OQQ66_004083"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1906781.1"
                     /translation="ISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFD
                     QVVFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQE
                     HGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFI
                     VQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGD
                     VLVTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGE
                     NVTVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEG
                     VGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIA
                     TLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCF
                     ALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCE
                     IPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAI
                     RAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          433 a          476 c          582 g          426 t
ORIGIN      
        1 atctcttatc gtgtgcacca gggttacgat caccgtggcg tggcgctctc cgccggtgtt
       61 cagcggatgg tgcgctccga cctcgcatct tctggcgtga tgttctccat tgataccgaa
      121 tccggctttg accaggtggt gtttatcact tccgcatggg gccttggtga gatggtcgtg
      181 cagggggcgg ttaacccgga tgagttttac gtgcataaac cgacactggc ggcgaatcgc
      241 ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc
      301 caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc
      361 tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac
      421 tacggtcgcc cgatggatat tgagtgggcg aaagatggcc acaccggcaa actgttcatt
      481 gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg
      541 cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat cggtgcgggt
      601 ccggtgaaag tcatccatga catcagcgaa atgaaccgca tcgaacctgg tgacgtgctg
      661 gtcactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc tgccatagtc
      721 accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact gggtattccg
      781 gcggtcgtgg gctgtggtga tgcaacagaa cggatgaaag acggtgagaa cgtcactgtt
      841 tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt tagcgtgaaa
      901 agctccagcg tagaaaccat gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac
      961 ccggatcgag ctttcgactt cgcctgtctg ccgaacgaag gcgtgggact tgcgcgtctg
     1021 gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga gtttgacgat
     1081 caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga ttctccgcgt
     1141 gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc gttttatccg
     1201 aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa cctggtcggt
     1261 ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg gcttccgtgg cgcgggccgc
     1321 tatgtttccg acagcttccg cgactgcttc gcgctggagt gtgaagcagt gaaacgtgtg
     1381 cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg taccgtagac
     1441 caggcgaaag cggtggttga agaactggcg cgtcaggggc tgaagcgtgg tgagaacggg
     1501 ctgaaaatca tcatgatgtg tgaaatcccg tccaacgcct tgctggccga gcagttcctc
     1561 gaatatttcg acggcttctc aattggctca aacgacatga cgcagttggc gctcggtctg
     1621 gatcgtgact ccggcgtggt gtctgaactg ttcgatgagc gcaacgatgc ggtgaaagca
     1681 ttgctgtcga tggctatccg tgccgcgaag aaacagggca aatatgtcgg gatttgcggt
     1741 cagggtccgt ccgaccacga agattttgcc gcatggttga tggaagaggg gatcgatagc
     1801 ctgtctctga atccggacac cgtggtgcaa acctggttaa gcctggctga actgaagaaa
     1861 taaaataaat acccggcggc gttttgtcgc cgggtttatg tgatccccga agatgaa
//
LOCUS       DAJVTY010000283         1909 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVTY010000283 DAJVTY010000000
VERSION     DAJVTY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.287"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            125..969
                     /locus_tag="OQQ66_004084"
                     /pseudo
     CDS             125..969
                     /locus_tag="OQQ66_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1084..>1909
                     /locus_tag="OQQ66_004085"
     CDS             1084..>1909
                     /locus_tag="OQQ66_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS1906782.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWL"
BASE COUNT          512 a          484 c          515 g          398 t
ORIGIN      
        1 ctgtataaaa accgaccatg cagagagaga aaacaccgga gtggagggaa aagcagaaaa
       61 gctcacgtgg cataaggcgc ggacagcggt atcgtctggt ttttcaattt cctatacgtg
      121 aactatggct cgctacgacc ttcccgatga ggcatggacc atcatcaagc ctctgttacc
      181 tcctgaaccc gccacaccac gggccggacg cccatgggct gagcaccgta aaatcatcaa
      241 tggcatgttc tgggtgttat gttccggtgc accatggcgc gatttacccg aacgatatgg
      301 ggcatggaaa actgtttata accgctttaa ccgatgatca aagtcaggcg tgattaatat
      361 tattttcaac aggttgcttt cgctacttga tgcaaacggc tttattgact ggtctgccac
      421 cgcgctggat ggcagtaata tccgggcgct gaaatgtgcc gccggtgctc aaaaaaacat
      481 cccgatatcg acggagataa tggcccgggt cgctctcgcg gcggttttgg caccaaaatc
      541 catctggcaa cagacggaag tggcctcccg ttaaatatcg tgctaagccc cggacaagct
      601 catgaaagcc agttcgcgca acgacttctg gacgggattg gcgttcagcg tcagaacggc
      661 agcatgaaac gccgtggaca tgcggtactg gctgataaag cgtattcagg gcgcgcattg
      721 cgcaacgagc tgaaaaataa cggtataaag gcagtaatcc cccgaaagtc aaatgagaaa
      781 atggcatcgg atggacgtgc acagcttgat cgtgatgcgt actgcaatcg taatgtcgtt
      841 gaacggtgct ttgggcggct gaaagaatat cgccgcatcg ccacgcgtta cgacaaaacg
      901 gcgagaaatt acctggcgat ggtgaaactg ggctgcatcc gactctttta tcaacgctta
      961 cgtaattaag ggacacagcc taaatagctg cgcggaatag tagatccctt tgagggaact
     1021 tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa caaatcacca accggactga
     1081 gcaatgccga tcatagcacc aatttcccgt gacgaacgac gcctgatgca gaaagccatc
     1141 cataaaacac acgataaaaa ttatgcccgc agactgactg ccatgctgat gctgcaccgg
     1201 ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg cccgttcctc tgttggacgc
     1261 tggattaact ggttcacgca gtcgggtgtt gagggactga aatcattacc tgccgggcgt
     1321 gcccgtcgct ggccgtttga gcatatctgc acactgttac gtgagctggt aaaacattct
     1381 cccggcgact ttggctacca gcgttcacgc tggagtacag aactgctggc aataaaaatc
     1441 aatgagataa ccggttgcca gttaaatgcc ggaaccgttc gccgctggtt gccgtctgcg
     1501 gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa
     1561 aagatggcag caatccataa agcactggac gaatgcagcg cagagcatcc ggtcttttat
     1621 gaagatgaag tggatatcca tcttcatccc aaaatcggtg cggactggca actgcgcgga
     1681 cagcaaaaac gggtggtcac gccgggacag aatgaaaaat attatctggc cggagcgctg
     1741 cacagcggga caggtaaagt cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc
     1801 atcagcctgc tgaagcggct taaagcgaca taccgtcggg cgaaaaccat cacgctgatc
     1861 gtggacaact acattatcca caaaagccgg gaaacacaga gctggctga
//
LOCUS       DAJVTY010000284         1850 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVTY010000284 DAJVTY010000000
VERSION     DAJVTY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.289"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..630)
                     /gene="csgD"
                     /locus_tag="OQQ66_004086"
     CDS             complement(<1..630)
                     /gene="csgD"
                     /locus_tag="OQQ66_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1906783.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWA"
     gene            1409..1600
                     /gene="csgB"
                     /locus_tag="OQQ66_004087"
                     /pseudo
     CDS             1409..1600
                     /gene="csgB"
                     /locus_tag="OQQ66_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
     gene            1641..>1850
                     /locus_tag="OQQ66_004088"
     CDS             1641..>1850
                     /locus_tag="OQQ66_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1906784.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTI"
BASE COUNT          568 a          351 c          384 g          547 t
ORIGIN      
        1 tgcccaggaa accgcttgtg tccggttttt tacggctatc ttcttgaaaa gattataaag
       61 atgcgtttta accgtatttt cgctgatgaa caacgaacga gcgatctcgt tattagacgc
      121 gccgatacgc agcttattca ggatctcttt ttcccgatga gtaaggaggg ctgattccgt
      181 gctgttataa cgatagttac ctgaatgcgt aatcaagtag ctggcaagct tttgcgtaaa
      241 gtagcattcg ccgcgcagga cgccttgcaa cccattgaca acacgttctt gatcctccat
      301 ggaataaaaa acgccgttga tatgaggcca gttttcaatg tcgcggtacg ggtaatcttc
      361 aggcgtattt agcaacaata ttttgatatt gttgtttttc ctgctcaaag tatcctgcca
      421 ataatggata agctttttat ctgcttccat catatccaga agaataatag agcctgaaga
      481 gatatcgtcc agagaacgtt gaatattatg taattttcct gtaattgcca gcgattgttt
      541 aaggtgctgc aagagagctg tcgcctgcaa agaaggttta gtgatcaaca ataatgtatg
      601 accatgaata ctatggactt cattaaacat gatgaaaccc cgcttttttt attgatcgca
      661 cacctgacag ctgcctctaa aatagaagca ccagaagtac tgacagatgt tgcactgctg
      721 tgtgtagtaa taaatcagcc ctaaatgggt aaaatataaa actaatggat tacatctgat
      781 ttcaatctag ccattacaaa acttaaatca agtgttaaac atgtaactaa atgtaacttg
      841 ttatattaaa atattaacct taaggtttta ttaagtttag aaatgataga aaagttgtac
      901 atttggtttt tattgcacaa ttttaaaaaa tcatacaaat ggtgataact tactaataat
      961 acatataaaa aatattttgg tgtagtcctt tcgtcatgta aaacgttctt gttttttctc
     1021 cgcacctccg tggacaaatt tttactccaa aaagacgagg tttgtcacgg cttgtgcgca
     1081 agacatatcg cagcaatcag cgacgggcaa gaaaaatgac tgtctggtga tttttgatag
     1141 cggaaaacgg agatttaaaa gaaaacaaaa tattttttgc gtagataaca gcgtatttac
     1201 gtgggtttta atactttggt atgaacaaaa aaagaaaaat acaacgcgcg ggtgagttat
     1261 taaaaatatt tccgcagaca tactttccat cataacgcag cgttaacaaa atacaggttg
     1321 cgtagactgg ccccctgaat ctccagacaa ccagtatcac ttatttaagt gatactggtt
     1381 gtctggagat tcagggggcc agtctaataa ccttgcatat attgatcagg cgggcagtgc
     1441 caacgatgcc agtatttcgc aaggtgctta tggtaatact gcgatgatta tccagaaagg
     1501 ttctggtaat aaagcaaata ttacacagta tggtactcaa aaaacggcaa ttgtagtgca
     1561 gagacagtcg caaatggcta ttcgcgtgac acaacgttaa tttccattcg acttttaaat
     1621 caatccgatg ggggttttac atgaaacttt taaaagtagc agcaattgca gcaatcgtat
     1681 tctccggtag cgctctggca ggtgttgttc ctcagtacgg cggcggcggt aaccacggtg
     1741 gtggcggtaa taatagcggc ccaaattctg agctgaacat ttaccagtac ggtggcggta
     1801 actctgcact tgctctgcaa actgatgccc gtaactctga cttgactatt
//
LOCUS       DAJVTY010000285         1823 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVTY010000285 DAJVTY010000000
VERSION     DAJVTY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.290"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(102..383)
                     /locus_tag="OQQ66_004089"
                     /pseudo
     CDS             complement(102..383)
                     /locus_tag="OQQ66_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(530..1390)
                     /locus_tag="OQQ66_004090"
     CDS             complement(530..1390)
                     /locus_tag="OQQ66_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCS1906785.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1573..>1823)
                     /locus_tag="OQQ66_004091"
     CDS             complement(1573..>1823)
                     /locus_tag="OQQ66_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1906786.1"
                     /translation="GQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVD
                     RNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          474 a          485 c          408 g          456 t
ORIGIN      
        1 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
       61 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
      121 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
      181 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
      241 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
      301 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
      361 tcctttccct gctctgccat aaggggtctg acgctcagtg gaacgaaaac tcacgttaag
      421 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      481 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      541 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      601 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      661 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      721 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      781 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      841 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      901 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      961 tcggtcctcc gatcgttgtc agaagtaagt tggcagcagt gttatcactc atggttatgg
     1021 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1081 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1141 cgtcaacacg ggataatacc gcaccacata gcagaacttt aaaagtgctc atcattggaa
     1201 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     1261 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     1321 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cgaaaatgtt
     1381 gaatactcat actcttcctt tttcaatatt attgaagcat ttaccagggt tattgtctca
     1441 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1501 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     1561 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ttataaaccg tggagcgggc
     1621 aatactgagc tgatgagcaa tttccgttgc accagtgccc ttctgatgaa gcgtcagcac
     1681 gacgttcctg tccacggtac gcctgcggcc aaatttgatt cctttcagct ttgcttcctg
     1741 tcggccctca ttcgtgcgtt ctaggatcct ccggcgttca gcctgtgcca cagccgacag
     1801 gatggtgacc accatttgcc cca
//
LOCUS       DAJVTY010000286         1817 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVTY010000286 DAJVTY010000000
VERSION     DAJVTY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.291"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(46..210)
                     /locus_tag="OQQ66_004092"
                     /pseudo
     CDS             complement(46..210)
                     /locus_tag="OQQ66_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(445..1290)
                     /locus_tag="OQQ66_004093"
     CDS             complement(445..1290)
                     /locus_tag="OQQ66_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCS1906787.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISSMTESIGGKTSLDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRGDRWGLHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENIIIRLDDHIRSVQGKACYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     MLPSL"
     gene            complement(1375..1572)
                     /locus_tag="OQQ66_004094"
     CDS             complement(1375..1572)
                     /locus_tag="OQQ66_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCS1906788.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAWEDIRTLRQLQLPDQNR"
     gene            complement(1592..>1817)
                     /locus_tag="OQQ66_004095"
     CDS             complement(1592..>1817)
                     /locus_tag="OQQ66_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906789.1"
                     /translation="QTNDDGETEWIHFTGSGYLLRTDAWSYPVLRLKRLGLSRTFRRL
                     VVTLTRRYGVSLIHLDAGAECLPGLPTFDW"
BASE COUNT          468 a          426 c          448 g          475 t
ORIGIN      
        1 aaaagggtgt tatggagcgt gtaaaacaac atcttcacct taatggcgat acagttggct
       61 gagagccagc agggggcaaa tcacctgcaa ataaacacgg cacatgctgg cgcgcatatc
      121 gcggatgcgt tcgaggacag aagaaccgac aggcggattc ttcagccgct catcgtccat
      181 cacaaaacct tagaccaggt attcctgaag ccgattgaat attgagcagt agaaaatatc
      241 tggattgaca gtattacaag gggttggaga acgtcagaag aggcattttg gcggaagatc
      301 acaggagtcg aatatgagta ttaatatatt gattttaaat gattaatctg tgttggataa
      361 atgaataacc cccataagaa cccccaaata aatttgcgtg agggaatgaa gctaaaatac
      421 ataattgaat tcattcttag agctttataa tgaaggtaac atttccgaat agtgcctggc
      481 aataatatca tttagccgat caaccagttc tggtttcctg aacgtgatgt gtgcggagcc
      541 cttcttaaag tatctgatgt tgaacatctc atcttcatag cacgctttgc cctgaacgga
      601 gcggatgtga tcatccagcc gaatgatgat attttcccgg ttgtcaggga ttggtttgcc
      661 actgaacaga tacagcattc tttccaggtc ggcgagtcga tctgcctgtt gcccggtgat
      721 aagatgcaat ccccaccggt cccccctcac cagattgttg acgataattt tactgccaaa
      781 tttacagggg gagttggttt tgtaattcca gctcagccct ctgaagacgt tgatcactcc
      841 tcgctcaaac acttcatcct tattctggtg cagttgctca aatgtgctcc aaatgttcgc
      901 ttcactgatt tccggaaagt tatcgtactc cagtgagcgg taccatgtat cacgggcctg
      961 cgcatccatt aatgacagca tcccggaacg ttgcatcagg tcacgccaga tttcgcggtc
     1021 aagattgcgg gtgatggctt tcattgcggt ttccggtttc tccatgagcc agcagccaca
     1081 gcgaaaatcc tgtttcatgg cccagtccag agaagtttta cctccaatac tttctgtcat
     1141 tgatgagatt tcagccagtt gccggataag tgtgtcgatt tgcagcagtg tagtctttcg
     1201 cacagccaga atgtggctga ctgaggtgga gcagataacg tccgtatgcc cgttgaccac
     1261 ttccggacgg gagactgtga ttgcagacat aatcgatatt ccttttcgca gataaatgaa
     1321 aacgccagcc ctgttcaggc tggcgtcagt ggaatgaagt atggtgaatg agactcaccg
     1381 gttctggtct ggaagctgca actggcgcag ggtacggata tcttcccagg cctgttcaat
     1441 acagggcagc agtgctgccg aatccgtttt atcctcatcg ttgtcgaaga taatccctga
     1501 ttcgcagtca atattgtcct gcagccacgc aatcagaata tccagcgccg tttctgtggt
     1561 taataatttc atccagtgtc ttttgtcctg attaccagtc gaaagtgggg agccccggca
     1621 gacattctgc gccggcgtcc agatgaatga gactgacgcc ataacgccgg gtgagagtga
     1681 cgaccagacg acggaacgtt ctggacaggc ccaggcgttt aagccgcaga accgggtacg
     1741 accatgcatc ggtacgtaac agatagccgc tgcctgtaaa atgaatccat tctgtttcgc
     1801 cgtcgtcatt agtctgg
//
LOCUS       DAJVTY010000287         1655 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVTY010000287 DAJVTY010000000
VERSION     DAJVTY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.292"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(2..952)
                     /gene="corA"
                     /locus_tag="OQQ66_004096"
     CDS             complement(2..952)
                     /gene="corA"
                     /locus_tag="OQQ66_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1906790.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1322..>1655
                     /locus_tag="OQQ66_004097"
     CDS             1322..>1655
                     /locus_tag="OQQ66_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906791.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
BASE COUNT          429 a          427 c          415 g          384 t
ORIGIN      
        1 attacaacca gttcttccgc ttaaagtaca gatacggtgc caggcccgcg aggatcataa
       61 agataatcgc gccagggtag ccgaagctcc acttcagttc tggcataaac tcaaagttca
      121 tgccatagct ggaagcaacg agcgtcggcg gcaggaatac cacggatacc accgagaaga
      181 ttttgatgat gcggttctgc tcgatgttga taaaacccat cgccgcctgc atcaggaagt
      241 tcaccttctg gaacagagat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt
      301 cacgcgcctg ctccagttgt ccacccggta agcgtgcttt acgtaccagg aagttgagcg
      361 cgcgctgggt atccatcaga cacaggcgta ctttccagcc gatatcttcc agttccgcca
      421 gcgtggagag cgcctcatcg tactcatcgc cctgatgccc ttccataatc acccggctca
      481 actgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt
      541 cgaacagatc cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac
      601 gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat
      661 cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga
      721 agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt
      781 cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtaca cgcagtcgct
      841 cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt
      901 cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga
      961 ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag
     1021 accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag
     1081 gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag
     1141 tatctactgg gtgtgtccaa ggcgaatgtc ctcttagaat gatcgtgcgc gcatgttacg
     1201 ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc
     1261 gctttttggt actcctcaag gtcgctaaca ggataaaaaa atcccacatc caggacctat
     1321 aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt
     1381 tctcaccctc accttacttc ccttgttttc tgttgccgct gatgattgcg cactctcaga
     1441 tccgacgctg accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta
     1501 ctggcaaaaa gccaatggcg aagcgtgggg aacgttacat gctctgctgg cggatattaa
     1561 tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc
     1621 gctcggtttg tacatcgaaa acggtcagca gaagg
//
LOCUS       DAJVTY010000288         1649 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVTY010000288 DAJVTY010000000
VERSION     DAJVTY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.293"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            6..1261
                     /locus_tag="OQQ66_004098"
                     /pseudo
     CDS             6..1261
                     /locus_tag="OQQ66_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
BASE COUNT          541 a          258 c          338 g          512 t
ORIGIN      
        1 tatatatggg tagtaatata catggaatta gttgcactgc aaataattat ttgaaacagg
       61 cctggaacga tataaaaaat gagtacgaaa aaaatcaaac atattcaatc acgctttttg
      121 aaaacacact ggtgtgtttt atgcggttat acaatgaact cagacgtaaa gtaaatgaag
      181 aggatactcc atgtctggaa tgtgaatcac tagaaaaaga atttgaggaa atgcagaatg
      241 ataatgatct atcattattt atgagaacat tgcgtactaa tgatacacaa atttattcag
      301 ggtttcagga ggtattacat atactataca atatgttcaa aatgttgata ttgttagagt
      361 gtccttgccg ggcagaggtt cagagtctat cacagatttt aaaggttatt attggtatgg
      421 ctttatggaa tatattgaaa acattactgc gtgtgatgat gttttttctg agtattgttt
      481 agatgatgaa aatatgagta tccagccaga acagataaat acgccgggaa tatctgattt
      541 agatactggt attgatttgt ctggcatatc ttttattcag agtgaaatta ataaaacata
      601 tggattaaaa tacgctccag tagatggcga tggatattgt ctgttaagag ccatactggt
      661 tttaaaagaa catgagtatt catgggcgct gggcggccat aagacgcaaa agcaagtata
      721 cgaagagttt attaaaattg ttgataaaca aacgatcgag gctcttgttg atacggcatt
      781 ctataatctc agggaagatg taaagacgtt atttggcgtt aatctacaat ctgacaacaa
      841 aattcagggg cagggtggtt ttttatcatg gagttttttg tcttttaaaa aagaatttat
      901 tgatagttgc ttgaatgaca aaaaatgcat actgcattta cccgaattta tatttaatga
      961 taacaaggta cgccttgttt tagacaccga cccagagcag agggttaatg aggtgaaaaa
     1021 tttcctcact gctctttcag atagcatttg ctcattattt attgttaata gtaatatggc
     1081 atcaatctcc ttggggaatg aatccttttc aacagatgac gaccttgagt atggttattt
     1141 aataaacact ggcaatcatt atgatgttta cctccctcct gaactttttg ctcaggctta
     1201 taagttaaaa aataaggaaa tgaatgcgca actcgactat ttaaatcgtt atgcaattta
     1261 atggcaaagg catatgctaa aaaccattgt tattagtctc acactttttt attggtaaat
     1321 attgtctctg tattggtaac gccgcagata ttctgtttag ccacaggtgc aattatcagc
     1381 ggcgtacgcg aggcaggggc taatcaggca tagtttgcgt caaaccttgc ctgtttttga
     1441 agatgtatat agaaaaacag gcgttcaaca agccattttg cgaacctgtt cccggaaaaa
     1501 agtcatattt ctgtcacact ctttagtgat tgataacaaa agaggtgcca ggaatgaaca
     1561 aaacgctaat cgccgcagct gtggcaggga tagttttact cgcttcaaac gctcaggcac
     1621 aaaccgtacc ggaaggctat cagctacag
//
LOCUS       DAJVTY010000289         1575 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVTY010000289 DAJVTY010000000
VERSION     DAJVTY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.294"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            148..669
                     /locus_tag="OQQ66_004099"
     CDS             148..669
                     /locus_tag="OQQ66_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906792.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            672..1217
                     /locus_tag="OQQ66_004100"
     CDS             672..1217
                     /locus_tag="OQQ66_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1906793.1"
                     /translation="MLHLKNITAGNPKTAEQYQLTKQYDVTWLFSEDGKNWYEEQKNF
                     ASDTIKMVYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     AITDKSSYNAIEWPVSPEGSS"
     gene            1241..>1575
                     /locus_tag="OQQ66_004101"
     CDS             1241..>1575
                     /locus_tag="OQQ66_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1906794.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVA"
BASE COUNT          431 a          340 c          424 g          380 t
ORIGIN      
        1 tgaaatatcc atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct
       61 gggtatcggc gttcagaacg ccctgggggg aattcaattg ctttcgggga taacgataca
      121 ggtataaaac aaaacggcga cggcattctg gatgtttatg cgaatggaca gcatgtattt
      181 cgtttccaga atggtgtggc gatagcgtta aaaaatattc aggccggaaa tgctaaaaaa
      241 ttcacgttat ccagcgccaa caactccacg aaaaatgcag cgttttattt gtggggtaat
      301 ccatccaggc ctgttgttgc agagcttggt gatgattccg gctggcattt ctacagccag
      361 agaaataccg atggtagtat cacattcgct gtaaacggac aggtaattcc gttaaattac
      421 ggaaacttcg atgcccgcta taaacatcga actgaggggg tacaggatgt gcggtatggt
      481 tatgaaatgt attacaaccc tggcagcaac accgtttcat ggacgtttcg ctcaccttcg
      541 ggacacgggt tgtcagggat atcgatatcg gataccggcc gtaactcagc ggataacgtc
      601 aacggtgtgt attaccgacc gctgcaaaaa ctgattaatg gcatctggta taacgtagcg
      661 agtatttaac aatgttgcat ttaaaaaata ttacggcagg taatccgaaa accgcggaac
      721 aatatcagct gacaaaacag tatgatgtca cctggctttt ttcggaagac ggcaaaaact
      781 ggtatgaaga acagaagaac tttgccagtg acaccataaa aatggtttac accggagacg
      841 ggcgcgtggt gtgggtcggt aaggatgtga caggcattga accccgtaac gccagtgtta
      901 ttgaagttcc tgatattacc gccaaccgcc gcattaccgc gccgggttac tggttttacc
      961 gcaatgatga atttgtcttt gactacagac tcaaagcgga agatgagcgt gatgcccttc
     1021 tggctcaggt cagtgcccgg acaggggaat gggaagaaga cctgctgctg gggctaatca
     1081 gcgacgaaga ccgggaaaaa ctgaaagcgt accgtattta cgcgaaatcg ctgcaggcga
     1141 tggatttcag cgccatcact gataaatcct catacaacgc cattgaatgg cccgtctctc
     1201 cggaaggttc ttcctgattt aatttatcgc gagaaaaact atgtctgtag tgatatcagg
     1261 tgcgctgact gatggcgcag gcatccccat gtccggatgc cagattattc tgaaatcccg
     1321 tgtaaacacc tcagaagtgg tgatgcgtac cagggctgat gtagtgaccg gaaataacgg
     1381 tgaatattcg tttgaggcac aggtcggaaa atattgcgtg tatctgaaac gggactggcg
     1441 cgacgagtac tgtgttggcg acattgctgt atacgacgac tcaaagcccg gcacgctgaa
     1501 tgactttctg actgcccccg atgaaggcga cctgaagccg gatgtcgtca aacgctttga
     1561 ggaaatggtg gcgca
//
LOCUS       DAJVTY010000290         1562 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVTY010000290 DAJVTY010000000
VERSION     DAJVTY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.295"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            60..381
                     /locus_tag="OQQ66_004102"
                     /pseudo
     CDS             60..381
                     /locus_tag="OQQ66_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(415..621)
                     /locus_tag="OQQ66_004103"
     CDS             complement(415..621)
                     /locus_tag="OQQ66_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906795.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1119..1192)
                     /locus_tag="OQQ66_004104"
     tRNA            complement(1119..1192)
                     /locus_tag="OQQ66_004104"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Gly,seq:ccc)
     gene            complement(1271..>1562)
                     /locus_tag="OQQ66_004105"
     CDS             complement(1271..>1562)
                     /locus_tag="OQQ66_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1906796.1"
                     /translation="TPIYAAGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTML
                     VNNGQSVKTGQKIATMGSTDAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          426 a          357 c          341 g          438 t
ORIGIN      
        1 gcattaatct aaaaacgctt taaatcaata cataaccatt atctcttggg attatttaca
       61 tgcgcaaaag tttaatgctg ttacttgctt caacattgat tatttctgga tgtgcaacaa
      121 aaaaacaact cgttccaact ggtggaagca aatcagacgg cacagttagg atgagttatt
      181 cttacggtat gtttgaaagt cctgttgtag atccacaaca aggcatgaat gcagccaaag
      241 cgcgttgtgc cgcttagggg atattcagga gccgagcctt ttggtggatt cacttctcaa
      301 tgttcacaac cttcttcatc tggttgtatg caaacacttg tgactatcga atatcaatgc
      361 actggcgata ttaagaagta ataattaggg gcttccagcc ccttctataa ccattcatgg
      421 aacagggaca accgaaatca ctttaaaccc tgaaacagcc gttactccat tagatggaga
      481 attaaatctc acagtatctt ttcttactgc atcatgaatt tcatcttttg ttggttcatg
      541 ctctgaatgg aataaaacct cttccattct aatttgatca ccgtctctat gctcataagt
      601 aatcttgtag ctaacagtca tatcaacctc atgcagggga attgtgagat tattatagac
      661 aatagaaacc ataaagcccc gttggtgata cagcaacaag cgggagcgta gcgacttcat
      721 cagattgtca gaaagatcac accttatcag gataaccgtg tacccgttgg gagcgtggct
      781 gaaacgctga tagagcttgc atatggtttg caaggaccaa tatcaccata aacctgttta
      841 cgcagccaca aggccgcaca tcgggcttaa atgcgtgtgt tcagtctgtc gatgacatta
      901 caatgccagg atgggcttta tcttcacgtt gtgctatcgc ggtgtgagag gtgcaactgc
      961 cgccaacgtt aggttatact gtcatctcac cgtgtaactt aacgtgacag ttactaaggg
     1021 cggatcacaa tggtagaact cctaagcaaa ttaaataggt aactgtttaa gttgctgaca
     1081 gagaagactt ttttagtctt ctccacctgg ggagattctg gagcgggcga agggaatcga
     1141 accctcgtat agagcttggg aagctctcgt tctaccattg aactacgccc gcttcgagat
     1201 gcgtaaggca ttataaacct tacgctctcc ttagcaagtg ccgcgctgct gactgctgat
     1261 taattcgcca tcagcatttt ggcttgctgc cctgcggcgg caagtaacgt agcggatcaa
     1321 ttgccgttgc acggtaacga atctggaaat gcaggcgaac agatgccgca tccgtgctcc
     1381 ccatagtggc gattttttgc ccagtcttca cgctttgccc attatttacc agcatcgtgt
     1441 cattatgggc gtaagccgta atgtaatctt cactgtgttt aatcatgatg agattaccgt
     1501 agccacgcag ctggttgccc acatacacca cctttcctgc acccgcggcg taaataggtg
     1561 ta
//
LOCUS       DAJVTY010000291         1527 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVTY010000291 DAJVTY010000000
VERSION     DAJVTY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.296"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..1386)
                     /locus_tag="OQQ66_004106"
                     /pseudo
     CDS             complement(1..1386)
                     /locus_tag="OQQ66_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          525 a          266 c          224 g          512 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatca ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaagtac tataaaa
//
LOCUS       DAJVTY010000292         1487 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVTY010000292 DAJVTY010000000
VERSION     DAJVTY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.297"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..1231)
                     /gene="sbcB"
                     /locus_tag="OQQ66_004107"
     CDS             complement(<1..1231)
                     /gene="sbcB"
                     /locus_tag="OQQ66_004107"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1906797.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYR"
BASE COUNT          373 a          356 c          359 g          399 t
ORIGIN      
        1 cccgataatt gaacaacagc ttttcaatcc gtttatcaac aaaagtgata tccagtgccg
       61 gtaaattacg tggctcggtt tccagcacaa ttttcattgc tgcacgatct gcgtcactga
      121 aaaagccgtt ataaagctgt gcatccacgt tatctgtagg cgtaaacggt tcggcttccg
      181 cgaatatcgc caccactttt tcacgcactt gcggattttc acgcagaatt ttcaggttat
      241 cgaggcaatg ctgacgatta attcccagtc ggtcggcatc ttccgggcgt agcgtattcg
      301 cctgggccag caccggacat ttattgatgt gcaccagctt aaccggaacg gcggcgttat
      361 cgccaagatc ggtttttgcg gtatataaac gctcgcgcaa tgtgtcgcta tccagttcca
      421 gtaatggcga aatgtctcct gccaaatcca ccataattac agcattgcga ttttcaggat
      481 gccacgccag cggtgccacc cagctggtat tgccgcgcca tgccccaaac attccggaaa
      541 cgtgaaccag ggggggcatc tgcggaacat caatcaacgc catcagtttg tgtttattac
      601 gatgggtaaa gagataatca aacaggcgtg gctgacgcgt ttttaccagt ttcgccatcg
      661 caatagtggc gtacacatca gccatcgcat cgtgggcgtt gctatgttca ataccattcg
      721 ctttggttaa atgctcaagg cgaaagctcg gtagaccgtc atcattttca ggccagttta
      781 ttccttccgg gcgcagggca taacaggcac gcataacatc caacaaatcc cagcgcgagt
      841 tatcatgctg ccagctccag gcgtaaggat cgtagaaatt acgataaaaa acgttgcgtg
      901 tgacttcgtc gtcgaaacgc acattgttgt agcccagaat acaggtcttc ggtacggtaa
      961 aaagcgagtg aatacgggcg gcaaacgcgg cttcgttttc tcctttcgcc cgtgcttcct
     1021 gcggggtaat accggtaatt aatactgctc caggctgggg taaatagtca tccgcgggct
     1081 tgcagtaaaa gacttcgggt tcgccgatga cattgaattc gctatcggtg cgaatggctg
     1141 cgaactgtgc agggcgatct aacgcggggt gcgtgccaaa ggtttcgtaa tcgtgaaaga
     1201 ggaaggtagg ttgtttatct gtgtctgtca ttgtctagca ttgtccactg tagttaatta
     1261 actgtctgtt ataactcgaa aaacaaccat tttttcacct gagtatgtct acgaacgtca
     1321 aatatagttg tttactgtgc gctgttgtcc atgtgatatt gtgtaccaga tgtgtaccaa
     1381 accattattt ctgagcgtac caaatattat ttatggctat aagcgacaca aaactgcgta
     1441 gtatttatgg taaaccatat tcgggcccac aagaagtggc tgatgcc
//
LOCUS       DAJVTY010000293         1465 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVTY010000293 DAJVTY010000000
VERSION     DAJVTY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.298"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..245)
                     /locus_tag="OQQ66_004108"
     CDS             complement(<1..245)
                     /locus_tag="OQQ66_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1906798.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTT"
     gene            353..1063
                     /locus_tag="OQQ66_004109"
     CDS             353..1063
                     /locus_tag="OQQ66_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1906799.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSDEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(1066..>1465)
                     /gene="speG"
                     /locus_tag="OQQ66_004110"
     CDS             complement(1066..>1465)
                     /gene="speG"
                     /locus_tag="OQQ66_004110"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCS1906800.1"
                     /translation="QSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKG
                     LATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQY
                     RNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
BASE COUNT          406 a          319 c          351 g          389 t
ORIGIN      
        1 ctggtggtgc gggtgtagag aattttatgc gtatcattag cacaatgccc gacgacctgg
       61 gaatcaggct gatcaacctg gtcattgggt acaatactta atgtgaagct gctttcgggt
      121 acgccattat tgataatgcg ctgtgatata tcgctctgta tgcgctcaca ggatcccggc
      181 gcggcgagta ccgcgggtga ggcgagggcg agcagaagcg cggcacagca ggttgagagt
      241 ttcatcataa gctccttacg cgaagataac ttctttaagc atagcattta acgtgtaaag
      301 tactgtattt gctactatga ttgagaatca tctctactct ctggtgactg ttgtgaaata
      361 caaattacta ccatgcttac tcgcgatatt cctcacagga tgtgaccgca cagaggtaac
      421 actttcattt acccctgaga tggccagttt ctctgatgaa ttcgattttg atccgctgcg
      481 tggtccggta aaagatttca ctcagacatt aatggatgag caaggtgaag tgacgaaacg
      541 tgtttctggg actttgtcgg aagaaggctg ttttgattca ctcgaattac tggatctgga
      601 aaataatacc gtggtcgctc tggtactgga cgccaattat taccgtgatg ccgagacgct
      661 ggagaagaga gtacgtttac agggaaaatg ccagctagca gaattacctt ctgccggggt
      721 gagttgggaa accgatgata atggcttcgt gattaaagcc agcagcaaac aaatgcagat
      781 ggaatatcgc tatgatgatc agggttatcc actgggtaaa accacgaaaa gtaacgacaa
      841 aacattatct gtcagcgcca cgccatcaac ggacccgatc aaaaaattag attacacagc
      901 ggttacttta ctgaataatc aacgagttgg taatgtaaaa cagagctgtg aatatgacag
      961 tcacgccaat ccggtggact gtcagctgat cattgttgat gaaggagtaa aacccgccgt
     1021 cgaacgggtt tacaccatca aaaatacgat cgattattat taatgctatt gtgcggtcgg
     1081 cttcaggaga gtctgacccg gtgttttgtg ctctgccaga tactgatgct ggaatataca
     1141 catgcgaatg gcattacgat attgaccatt aataaaaaac tcatgcatca attcaccttc
     1201 aaccgtaaag ccaagcttgc ggtaaatgtg aatcgctttt tcattctctt tatcgacgat
     1261 cagatacagc ttatagagat tgagaacggt gaagccatag tccattgcta atttggcggc
     1321 acgggttgcc agacctttcc cctgatactc cggggagata attatctgaa attctgcccg
     1381 gcgatgtaca tggttaattt ccaccagctc caccagaccg gctttttcgc cgtcacattc
     1441 caccacaaag cgtcgttcgc tctga
//
LOCUS       DAJVTY010000294         1430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVTY010000294 DAJVTY010000000
VERSION     DAJVTY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.299"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..547
                     /gene="bluF"
                     /locus_tag="OQQ66_004111"
     CDS             <1..547
                     /gene="bluF"
                     /locus_tag="OQQ66_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCS1906801.1"
                     /translation="ALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANA
                     LVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDR
                     IKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQ
                     GDLFAKAKLNGIPSIAWPEKK"
     gene            752..>1430
                     /gene="bluR"
                     /locus_tag="OQQ66_004112"
     CDS             752..>1430
                     /gene="bluR"
                     /locus_tag="OQQ66_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCS1906802.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNE"
BASE COUNT          406 a          297 c          335 g          392 t
ORIGIN      
        1 ggcgcttgca ttcacgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa
       61 tctattacct atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttaatgaaat
      121 aaaggccaat gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagtcat
      181 atctcggttt gatgagtttg ccgaagcgat taaatcgcta aaggctgctg gtatcagtgt
      241 agcaattgat cattttggcg caggttttgc tggtttgtta ctcctgtcac gcttccagcc
      301 tgacagaatt aaaatcagtc aggaattgat taccaatgtt cataaaagtg ggccacggca
      361 ggcaattatt caggcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat
      421 gggcgtggca acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca
      481 gggagatctg tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa
      541 aaaataattt tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac
      601 gttacgttta aatttaatcc acattaaaaa taatgtttca tccaatttag ctgaaatctt
      661 atttatactt tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg
      721 cgacggagat ttgtgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc
      781 tgaacgttgc gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt
      841 aaaaccacag cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat
      901 cgaagagatc aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact
      961 ggaaaccacc agccaggata cggaagatga ctggagccgc ctgcaagaag agatgatgtc
     1021 aattcttcgc atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga
     1081 gtacccagta gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct
     1141 tgatcacaac acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatacgc
     1201 agcaacctcg cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg
     1261 gaatgttgaa gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg
     1321 gcacatttct gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca
     1381 aacattgatt gtctggacag gcatggcacc aacgagaagg caaaacgaac
//
LOCUS       DAJVTY010000295         1425 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVTY010000295 DAJVTY010000000
VERSION     DAJVTY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.300"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(66..332)
                     /locus_tag="OQQ66_004113"
     CDS             complement(66..332)
                     /locus_tag="OQQ66_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906803.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVEIVEKQSQRHSNI"
     gene            complement(514..912)
                     /locus_tag="OQQ66_004114"
                     /pseudo
     CDS             complement(514..912)
                     /locus_tag="OQQ66_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1235..>1425)
                     /locus_tag="OQQ66_004115"
     CDS             complement(1235..>1425)
                     /locus_tag="OQQ66_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1906804.1"
                     /translation="PRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFI
                     WDMGRIAMSVAQQPQCHK"
BASE COUNT          343 a          310 c          307 g          465 t
ORIGIN      
        1 attattagta acaaaccctg atatattctt ttgaaaatgg agcttaaacg agtctgaaat
       61 taacttcaga tattagaatg ccgttggctt tgtttttcaa caatttctac ttcatttatc
      121 tttgtcaacc attgcatttc tgtagcagtg aatttcaacg ttttcgtccg gttggtagca
      181 ggtatttgta attcgtatat agctgctttt ttattgaaaa cagtatatcc atctgtagta
      241 acagtagaag ataataatac aggatttgac acttcaagga tgatattttc ttgtttttgt
      301 ggaaggcaag ggaatatagt ttgtgtaagc atattgcttc cttatgttat attttagtaa
      361 agaatttatt ttttacatta tagtcggcta aatgaagttt actgtaaaaa aacgatactt
      421 ttttcgtaag cgatgagatg tggagccaga aaaatggctg cgttacgtca ttgaactgtc
      481 cttgacccac gatattcaga cagtcgttgt atcttaagtt gacattaccc gctgacgtcg
      541 atattccctt ggtgagagat aacccaacgc actatgcgga tggttttcat tgtaatgctc
      601 gatcgccact gcaaggttat gcaatgccgt tcttggtttc ggcatgaacg cgatgcagtc
      661 ttccttcatc gttttcacga accgttctgc tattccatta ctctgagggc tactgactgc
      721 cgtcgtgcag ggctccagat tcaactctct ggcgaactgc cgtgtttcat gagccgtata
      781 agccgaaccg ttatcggcca ctggattggc cgttccggtc ctttatcgcc atatcgctga
      841 catgacatcc tgcaccgttg ctttgtcata atctcccgtg cctgctgccc agtctatggc
      901 ttcacgatcg caatatggaa cgtaacgctg tcatcgggag aatcgtcaat gcggcattcg
      961 gttaacgctg atatgacttt ccgtgtcaaa ccggaatctc taattttgac gattttcttt
     1021 ttaggtttaa ttccctgagg gcgacgtagc agtaaatccg acggtgaatc acgctgatat
     1081 tcgcgggttg gataccgcat tacagaggtc cgtcatatga gcctgtcact ttcttacgtc
     1141 gcgaatcagc tttgttccgc cgtcgccaag cgctaacgtg ggttactctt gtaccgggct
     1201 acgtcgcctt cagggaactg gttattctca ttttttattt gtgacattgt ggttgttgtg
     1261 cgacagacat tgctattcgg cccatatccc agataaaacc cgccagctca cgtgcaacag
     1321 caacaattgt aatattgaca ttctttcctt tggcctgaag ttttcgatac ctcttacaaa
     1381 gcctgagttg agcatcccat gctctgtcaa tgacggggcg gggta
//
LOCUS       DAJVTY010000296         1409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVTY010000296 DAJVTY010000000
VERSION     DAJVTY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.301"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(498..965)
                     /locus_tag="OQQ66_004116"
     CDS             complement(498..965)
                     /locus_tag="OQQ66_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906805.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          311 a          304 c          360 g          434 t
ORIGIN      
        1 agtttagtct ccaggattcc cggggcggtt cagatggtcc ttcactggga tggtaatcag
       61 agacaggcta aaatgttgtc agcgcgtttc cggtgcattt tttgggataa tggttggttc
      121 ggggggggca ccgggaacgc gcttcagtca gaccttgctg aaaacacagg aacgcgttaa
      181 aacgcatacg ggcgcgattc tggtgtgtct tttgttgctg tgttttgcct ctattgccag
      241 atcgcgctca ttcccctgaa cttctgtttt cccgctcagg tccccgtctt cttcgcttcc
      301 gaaagcatga aaaaaatccg tttcagctgt ctctggctgg caattcctgc gtgattcaca
      361 tggctgcata gctatgcatg tggttaaaat ttaccgggtg cgatcgcggc agtttttcgg
      421 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgccgaacgc gtttcagcgg
      481 tgcgtacaat taagatatta cgttaaattg tgagggaatg gtccccatat actgacttta
      541 tcatagggat agggttggta gtgggtcgat tgggtttcgg cattgtttcc gttaatgcca
      601 attgcatttt tgtatttgat gcaacagaag gcgtgagatt ctcctgggcc tggaaaggct
      661 aagtgtttag aattgatggc aaaatatctt ccgtccgatt tttgaagtag ttgttgtttg
      721 gctgtctccc aaagggtgct gttacaaact tcttgaattt gtagtttttg cagggctctt
      781 attactgcgt tgtcatgttc caattcttta ggggagtgta ttattctctc ttttaataag
      841 ctgtttacca cagggtttag ttgttgtcta aagcacgcag ataatgccat tgctacacaa
      901 ttttgttccc gaggaagcat gtttggcggg gtatgccgtg tttgatgtga tatgtctatt
      961 ctcatagaag gtctctggct tatagcatca acaaatacgg ctataaaaca aaattttcat
     1021 agagtgattc aagatcttct tgagatcctt ttttgcggcg cgtaatctgc tgcctgcaaa
     1081 caaaaaaacc accgctacca acggtggttt gtttgccgga atctagagct accaactctt
     1141 tcttccgaag gtaactggct tggtggagca cagataccaa atgctgtcct tctagtgtag
     1201 ccgcagttag gccaccactt caagaactct taatatctca atccaccttg tacagttacc
     1261 aatggctgct gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1321 accggatgag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1381 gcgaacgacc tacaccgaac tgagatacc
//
LOCUS       DAJVTY010000297         1399 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVTY010000297 DAJVTY010000000
VERSION     DAJVTY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.302"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OQQ66_004117"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OQQ66_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1906806.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
     gene            complement(1183..>1399)
                     /locus_tag="OQQ66_004118"
     CDS             complement(1183..>1399)
                     /locus_tag="OQQ66_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1906807.1"
                     /translation="AEEQKYEMLENEYSQRVADRLKASGLSGDTDAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          378 a          285 c          298 g          438 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg
     1201 tgagccgttt tcagacaatc gcagggccag tacctcgtca gtcaactgac ggtaaatctg
     1261 ctgttcagtc tcacgcatca cctgtgcacc ggcttccctc tccgcatccg tatcaccgct
     1321 cagacctgat gctttcagcc ggtcagccac cctctgagag tactcattct ccagcatctc
     1381 atacttctgc tcttctgcc
//
LOCUS       DAJVTY010000298         1370 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVTY010000298 DAJVTY010000000
VERSION     DAJVTY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.303"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OQQ66_004119"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OQQ66_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1906808.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1370)
                     /locus_tag="OQQ66_004120"
                     /pseudo
     CDS             complement(1149..1370)
                     /locus_tag="OQQ66_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          458 a          222 c          246 g          444 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg
//
LOCUS       DAJVTY010000299         1358 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVTY010000299 DAJVTY010000000
VERSION     DAJVTY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.304"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..276
                     /locus_tag="OQQ66_004121"
     CDS             <1..276
                     /locus_tag="OQQ66_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906809.1"
                     /translation="SLWGPWHAVLKRTEADRWALAEEQKYEMLENEYPQRVADRLKAS
                     GLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(277..456)
                     /locus_tag="OQQ66_004122"
     CDS             complement(277..456)
                     /locus_tag="OQQ66_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906810.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(456..1079)
                     /locus_tag="OQQ66_004123"
     CDS             complement(456..1079)
                     /locus_tag="OQQ66_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1906811.1"
                     /translation="MYMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
     gene            complement(1030..>1358)
                     /locus_tag="OQQ66_004124"
     CDS             complement(1030..>1358)
                     /locus_tag="OQQ66_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906812.1"
                     /translation="IIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQS
                     YRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGGPDVHVSGAMPG
                     LFIRTS"
BASE COUNT          300 a          398 c          348 g          312 t
ORIGIN      
        1 tccctctggg gaccatggca tgctgtactg aagcgtacgg aagctgaccg ctgggcgctg
       61 gcagaagagc agaaatatga gatgctggag aatgagtacc ctcagagggt ggctgaccgg
      121 ctgaaagcat caggtctgag cggtgatgcg gatgcggaga gggaagccgg tgcacaggtg
      181 atgcgtgaga ctgaacagca gatttaccgt cagctgactg acgaggtact ggccctgcga
      241 ttgcctgaaa acggctcaca actgcaccat tcataatcac atcgcataaa ccacagaccg
      301 gactgactcc ggaaaaacag aggcccgccc ccgggcctcc ccggattcat ccgtttccct
      361 gttcagcctg acagcacgcc cccgcggccg gatgacagac tccgcttcgg taagcaaagc
      421 ggtcttctgt gattccgcca gttgcggctt attcattact caacgtcaaa cgcccgaatt
      481 gaagccaaat catccagacc gctcagctcc tctttcattt cacgctgacg gcgataaatc
      541 tcatcgttgc gatcgacctg cgcctgcacc attgctgccg ccagttcttc cagttccggc
      601 atcgacagtt tcacctgctg attatcggca tcgctccacg ccatatgtgt ttgtgctgtg
      661 acagattttg ccagcatgac taccggggac aggcggccca gtgagtcggg accagcattc
      721 cagatacgac cgttccattc aaacgtgaac ggcttcgcct cctgttctgc gcgccatgct
      781 tcaatttcct gacgtctggc ctctctggcc gcttccagca tttctggtgt cacagtgaat
      841 ggggctatct caccccattt gccgctttgc agttcctgcc agatttgctg acccgtcgga
      901 gcgacatcat cagcggtggc tgtgtagggg actgcctggt ccctgtcgtc aaaaaaaacg
      961 tcacagtcta ctgcgccact ttcggtataa cggggattaa tgattttttt aatttccacg
     1021 gtgcattcct cacgatgtgc gaataaaaag cccgggcatt gcgccagaga catgtacatc
     1081 cggccccccg gacagggcgc aatatgaccc cggtaatgaa tgctctgaac atcccgtaat
     1141 gaaaaattgt ggggatgcta tatacgttcc ggtgggagta ctgggcactg aaatccccac
     1201 cggtcccagt cgtgagcctc tgtatgactg ccccctgaca agtctgatga ctttatcacc
     1261 gtcagcttct ccctggtacg cagcaataat cagcccgcca atgtcagggt ctccccatct
     1321 gttgcggaca gagctcgcca cgattctgta aataatat
//
LOCUS       DAJVTY010000300         1349 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVTY010000300 DAJVTY010000000
VERSION     DAJVTY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.305"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(341..496)
                     /gene="hokD"
                     /locus_tag="OQQ66_004125"
     CDS             complement(341..496)
                     /gene="hokD"
                     /locus_tag="OQQ66_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1906813.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          340 a          332 c          293 g          384 t
ORIGIN      
        1 ttcttaatct cgtatccgtg tctgcggtag cgctgaatca gccattcagc ctgttcttcg
       61 gtgcaggggt cgtgctgata ccagtcagat ttgaatgcat gagaacgccg cccgtgcctg
      121 ctggcaggcg cggcagagtt atccgaattg taaaatttgg tatcgtgcgc catctgtttt
      181 ctctgctggc gcagcaggtg ccagttgttc aggctgacgg atggattgta aaccagaacg
      241 accagaaaaa acaaaacccg ccgaagcggg ttaagtgcgg gtgcgttgag gatgcctgac
      301 acatcagcgg tggcgaggga tttctccccc gccgggtctc ttactcctca ggttcgtaag
      361 ctgtgaagac agcgacctcc gtctggccgg ttcggattcg tacctcgcag aggtctttcc
      421 tcgttaccag tgccgtcact atgacggtta aacagatgac gataagggcg attaacatcg
      481 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cacgtaagag gctaacctac
      541 atgtgttcag catggattga gcctcagatt aatgttaagc gtcttgcagg acgcgtagtg
      601 ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgagacag atagcctcaa
      661 gcacccgcag tcattctact taactaagat ttccctgcaa accgtttttg tccggcacag
      721 taaatatcca actaaaccaa tagcgttcgc tgtatttacc gccagtattc aatgcacatg
      781 accgccatga acacccctaa aaaaagggca tttatatgtc caaacattaa tatcaaaaca
      841 tcaatttttt ccatatacct tgctgtgaag atgatgggca tacatgatgc gaacaaccag
      901 aacgcaacaa acaaaaactg caatgcgttt ttcattattc cccctacaat caatgtgcaa
      961 taacatttaa acacacctca atttggccgg acatataaat atctaaacca gaaaaaatca
     1021 cttacatagc gttacaaact ctttagtcta aatactcatc gtaaaacatt ccccatactt
     1081 atcagtccgt tccgcgccag gtagcttatt gccttatctg gcaacctata atcaggtttc
     1141 cgctttttca gttggctggt cgtttaaccg acatagttaa cccattaatc tggttgccgg
     1201 atgctggtgg attttcgcgt tttagttgtt cataaaagtg cacagcttta accagttctt
     1261 ctgatgtaac cgggactggt ggggcagtga ataaggcctg aatttcatag ttcggcctgt
     1321 cgttacaatc ctcttttttc ggtacatat
//
LOCUS       DAJVTY010000301         1320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVTY010000301 DAJVTY010000000
VERSION     DAJVTY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.306"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(63..335)
                     /locus_tag="OQQ66_004126"
     CDS             complement(63..335)
                     /locus_tag="OQQ66_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCS1906814.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(378..614)
                     /locus_tag="OQQ66_004127"
     CDS             complement(378..614)
                     /locus_tag="OQQ66_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCS1906815.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            928..>1320
                     /gene="bluF"
                     /locus_tag="OQQ66_004128"
     CDS             928..>1320
                     /gene="bluF"
                     /locus_tag="OQQ66_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCS1906816.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRT"
BASE COUNT          386 a          266 c          238 g          430 t
ORIGIN      
        1 ttattgcatt atgaattgta gtatcttcaa gcatggtaac ctctcatctt acttatgaaa
       61 ttttaatgta ttctgtttat tttcttacca ttgaacatac ttttattgtc agctgtcgct
      121 gttcttcggt ttggccctcc tgaatcacta ttgctaaaat cacgaattat gctgtcaatt
      181 aaatcgcgtc ggaaagcatc ttcctcagcc gatttcatta cgagtaattg cttaataaga
      241 gtctgcaatg aaaaatttat cttcatgcgc agtatctgga gcaccacctg gccagtaatt
      301 tcatatttta tacgttcatt atcagatgtc ttcataacga cttcatagct aacctgttaa
      361 ataatcaggc tgttgcatta ttcaaaaagc aacccaatta gtgcattata atgtttctgt
      421 tcctcttccc cagtcgcgtg ttccagtcgg caaagaagtt tggcacaaag cgactttcga
      481 ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt ttcctgctga
      541 gtaggccagt tagcttttgc aaagtaacga gtgattgctc ccgcagaatc taaagtcact
      601 gaattttgat gcatgctacg cctctgttaa aaatgttaat caatgttaag tgaggtaaca
      661 atagcaaact tgtacgaaat atgtgtacag aatgataaat taattttttg caattagtgc
      721 caatacatat aaaacaatgt gttatgataa aacatattgc atcatttaac tgacaatcta
      781 tacgagattt ttcgccaact agtgttcaac tttttccatt ttttatcaat gtaaagaaac
      841 ggttatgttt tggtattaaa agaaagacac aagaagatat gtctgttacc gtcttactct
      901 cgcctcaccc attaccctgg attgttaatg cttaccaccc ttatttatcg tagccatata
      961 cgtgacgacg aacctgtcaa aaaaatcgaa gaaatggttt cgatagcaaa tcgcaggaac
     1021 atgcagtctg acgtaacagg gatcttactg tttaatggtt ctcatttttt ccagcttctg
     1081 gaaggtccgg aagaacaggt taaaatgata tatcgggcta tatgccagga tccacggcac
     1141 tataatattg ttgagctgct gtgcgattac gcgcctgctc gccgttttgg caaagcggga
     1201 atggaattat ttgatttgcg cctgcacgag cgagatgacg ttttacaggc cgtattcgac
     1261 aaaggcacat caaaatttca gctaacttat gatgacagag cgctacaatt ttttcgtact
//
LOCUS       DAJVTY010000302         1308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVTY010000302 DAJVTY010000000
VERSION     DAJVTY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.307"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(421..957)
                     /locus_tag="OQQ66_004129"
     CDS             complement(421..957)
                     /locus_tag="OQQ66_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1906817.1"
                     /translation="MQTQNVVTDKEREIRKRDLEDKDRKNASERRQEQKNQNFTQVYP
                     LGWKRLRELFRKNPGAAELYSMLAENIDGSCGAVVADQTHLASLLGVGRQTISRYVKW
                     LEEQGVLVKIPVAGKVCAYALNPHEVWKGYDNAKPYAAFLTKTLVNKDGDIQRRIMAM
                     FHGNDARQGMEGDTPEAE"
BASE COUNT          311 a          318 c          307 g          372 t
ORIGIN      
        1 aaaaaaaatt ggggaaggtg gttgtgttca cgtagtgagc gcaaccacct gccagacggg
       61 ttttagcgcc gcaggccgca catgtttctg agccaacggc gaggaaacat ataagtgcgc
      121 atctctctaa tttcacgaat ttgagagatg agatcagtct ggataatcgg agtactccgc
      181 ctctagccgt cctggtgaac cagtccggca agacccacga aatcacccgt aaaacgcaat
      241 aaaacgcgtt ttgacgcgtt ttttgacgtt ctacgggctc acgtgggctt tgggcggaaa
      301 aatggcttac gtgcgttctg agagcgtttt agggctttgc agggtggagt ggtgaagcag
      361 ctccttgtgg aacctgtacc gcccttgcgg gcgggcttcg gcgatttggc gaacgggaat
      421 ctattcggct tctggcgtgt cgccttccat gccctgacgg gcatcgtttc cgtggaacat
      481 ggccatgatt cggcgctgaa tatcgccgtc tttgttgact agggttttcg tcaaaaatgc
      541 ggcatacggt ttggcattat cgtagccttt ccagacttca tgcgggttga gagcataagc
      601 gcataccttt ccagctactg ggatttttac taaaacacct tgctcttcta gccacttaac
      661 gtacctagat attgtttgtc taccaacacc aagcaacgat gctaaatggg tttgatcggc
      721 aacaaccgca ccacaagagc catcaatgtt ttctgcaagc attgaataaa gctctgctgc
      781 ccccggattc ttcctgaaaa gctctctgag acgcttccag cctaaagggt atacttgggt
      841 gaagttttgg tttttttgct cctgtcgacg ctctgaggcg tttttacggt ctttatcttc
      901 aagatctctt tttctgattt cccgttcttt gtccgtcaca acattttgag tttgcataga
      961 ctatctctcc tgcgacacca tgtcgtttga aagtaacata cagttgtcat ttttttgtgt
     1021 caacatgtcg catttgcacg acatgttggg tatatctgtc gcatcctata gatgcgacaa
     1081 cgttgtcgcc cctaggatgc gacaacacat ttataaaccc ataataaaaa caacaacata
     1141 caattttttc cttcttatct aaaaagatct cacagcgagc tgtgagatct ccaggtagca
     1201 ccctcctttc agtcgggcaa aaaacggtga aaatttaacc attggcaacc gaaaaatcgt
     1261 gggggcccct tcaggggttc cgattttttt gttgccaacg caccgatc
//
LOCUS       DAJVTY010000303         1290 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVTY010000303 DAJVTY010000000
VERSION     DAJVTY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.309"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>1290)
                     /gene="pdeF"
                     /locus_tag="OQQ66_004130"
     CDS             complement(<1..>1290)
                     /gene="pdeF"
                     /locus_tag="OQQ66_004130"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1906818.1"
                     /translation="WAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMA
                     VLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNY
                     GIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFR
                     FFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQ
                     RELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFL
                     PVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLL
                     AKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNV
                     NADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMR"
BASE COUNT          351 a          316 c          309 g          314 t
ORIGIN      
        1 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
       61 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
      121 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
      181 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      241 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      301 gacttcaaaa ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      361 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      421 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggct
      481 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      541 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      601 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
      661 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
      721 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
      781 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
      841 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
      901 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
      961 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
     1021 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
     1081 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctct
     1141 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
     1201 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
     1261 gtggatgctg atcatcaaca cgaccgccca
//
LOCUS       DAJVTY010000304         1264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVTY010000304 DAJVTY010000000
VERSION     DAJVTY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.310"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(26..1174)
                     /locus_tag="OQQ66_004131"
     CDS             complement(26..1174)
                     /locus_tag="OQQ66_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906819.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          385 a          358 c          288 g          233 t
ORIGIN      
        1 aagttcacac tctgcttgaa ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg
       61 cgttgcgcag cagcatcagc aaatagataa acgatacgct ggcgatcatg ataaacaata
      121 aattatcgga gaaattctgc attagcatag cggtaaatga cgggccaagc agacttccca
      181 cagtatagct caacagtaag gcctggttca tcgccaccag ttgatgatgt tcaactttct
      241 cgcaagccca tgccatcgcc accggatata gcgtaaagcc agcggcaccg aggatgaata
      301 acgctggggc catcgccgcc tggctaagca tcgcgatact gccgagaatg acgacaaaga
      361 cctgaacacg caacaccagc agtcgaccaa acttatccgc cagacgtcca atcgaccatt
      421 gtccaaggat acccgcactg accagtaccg ccatccagaa accaatgctg gcattgctca
      481 cccctttgtg attgaggtac agcggcatca ggccatatag agatcccaga acgattcctg
      541 agataatgca gccattcacg ccaagccgcg cctgacggag ttttagcatt gacgtaattg
      601 acgtcgaatc atggttttca gcctgctgat tcagcacacg cgtaaacaac agcggtaaga
      661 tccctgccaa cgtcaaacct gtaacccacg gcaatacgga catcagctcg gttgaaactt
      721 tgctgaccag taactggcct aaaaacgttc ccacgtaata aaccatcata tacgcagcaa
      781 gcaaacgccc acggttacgt gacgtcccac tgcacatcag cgcgctctca acaaccaccc
      841 aaatcatggc acagccgacg cccgcgacaa aacgccaagc caaccagctc cagaatccaa
      901 tcatcaggcc aaggccggca cagccagcgg caaaaatgaa ggaggccaga taatagctgc
      961 ggttaaagcc aatgcgctta atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg
     1021 taaaatagga tgagctgaca acgcctacct gccatgtgga catgtgttcc tgggcgagcc
     1081 aaagcggcac gagtgtattt aacaccgcaa tcgccagagt caacaaaagc aggccagaca
     1141 gcaaaagcat gacaggccgg gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa
     1201 tttcatgcgc atcatgccac cgccaaaaca ttgtcaatga gtgaaaatac gcgatatccc
     1261 agcg
//
LOCUS       DAJVTY010000305         1264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVTY010000305 DAJVTY010000000
VERSION     DAJVTY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.311"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(774..926)
                     /locus_tag="OQQ66_004132"
     CDS             complement(774..926)
                     /locus_tag="OQQ66_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906820.1"
                     /translation="MSDITAYRPEVVNENTDVICSTSIRRILAVRKSSLLQIDTIIRH
                     QVSDIS"
     gene            complement(1011..1208)
                     /locus_tag="OQQ66_004133"
     CDS             complement(1011..1208)
                     /locus_tag="OQQ66_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906821.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
BASE COUNT          352 a          296 c          279 g          337 t
ORIGIN      
        1 cctttgcacc agaataaacg ccagagacct gaaattgcga gtacactaca gaaattgtac
       61 tgccggtccc atcagcataa aaccatagtt acacaggtaa tcatcattat tcgccaagat
      121 aataaatatt attcggtaga atagcgttct caggtggtca gtaaaattgt cttcaacaag
      181 atgtctgcgc atctcgtctg ctagccctac ataactgctc tcgaccgaaa ctatttattg
      241 aatgttcacc aaggtgggcg tggagacaaa atgacgtctt ttggtattag ctacctgagc
      301 cacatggtca aaacgttcaa gtagcatagc gaaaccaccc tgcccacgta atgtggacga
      361 tgcagcttcc agccagagca aacttgggac catcgactac aaaatgaaga atactgaact
      421 gcttttctgg caggcctata tactgcacgg atatgggtga tttcagacac aaaaaaagcc
      481 gctcttgagc gactcgattt gcatacggtg tggtgcgaag gccggactca aacatgtgac
      541 ttaacctcat gatttaaaat tgttaataat aaacaatatc atcttatacc aacacagata
      601 ccaacacgaa aaatcactgg ttttcggcat cgaaccagga caatcagctc ctgattgaga
      661 taccgagctc tgttactcgt catcttcctc gacaggcttc attgcatcaa gtagtactcg
      721 caccatcgcg atagaaccaa tccggataaa agcatcttct ccatctatat ctgttaactg
      781 atatcgctca cttgatgccg gataattgtg tcgatttgca gcagtgaact ctttcgtaca
      841 gccagaatgc ggcggatgct ggttgagcag ataacgtccg tattctcgtt gaccacttcc
      901 ggacggtagg ctgtgatatc agacataatc gatattcctt tttgcagata aatgaaaatg
      961 ccagccctgt tcaggctggc gtcagtggaa tgaagtctgg tgagtaagac tcaccggttc
     1021 tggtgctgaa gctgcagttg gcgcagggta cggatatctt ctctggcctg ttcgatacag
     1081 ggcaacagtg ctgctgaatc cgttttatcc tcattgttgt caaagataat tcccgattcg
     1141 cagtcgatat tgtcctgcag ccacgcaatc agaatatcca gtgcggtttc cgtggttaat
     1201 gatttcatgt tgttcctggt taccagttga aagtgggtaa acccggcagg cattcagcgc
     1261 tggc
//
LOCUS       DAJVTY010000306         1263 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVTY010000306 DAJVTY010000000
VERSION     DAJVTY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.312"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..1263)
                     /locus_tag="OQQ66_004134"
                     /pseudo
     CDS             complement(1..1263)
                     /locus_tag="OQQ66_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          372 a          355 c          281 g          255 t
ORIGIN      
        1 tgccaaccag taaaacgctt cagttttcaa cgcaccccac agcatcggcc agaaaatgag
       61 tcctgcaatt gcccctgtgc gcataatagc gcgggcaaat ccgaccccag tagggcgaat
      121 agcgggaaga taagagagtg tggcaatggt catgcccagc ccaccaggac ccgctgaatg
      181 gaagaaaata attgatccca ataaccctaa agacagccag ggattagtcg ttgccagtgc
      241 gcctaacgac agcaatgcta aggtcacaac agcaaatcca tacatcgact gtcggcgagt
      301 acccagtcgt tggagaataa acgagccgat catcccgcct gtcaccccac aaagggcatt
      361 cacgacggca gaaccggtca acgcgccagt aagcccgcca cttattattc ccgccaacgt
      421 aagaggaaga taaacaccta cggcgttata ttgccacgcc tgcatggttg ccacgacaca
      481 gccaagaatg gtgcgtttgc ggtaacgatc attaaataag atgccatatc caccactgta
      541 tttattctct gctttttccg agaatttcgc ttctgttgta cccgatgccg caacgcgagc
      601 ctgaacacca taacgttttc ccagaatgtc gcacgcttcc tggtaacgcc cgacgcgtgc
      661 ggcccacatt gcggattcac caataaagaa gtaacgcaaa atcatgaaga tgactgcaaa
      721 aatagctccg atgaatattc cgtaacgcca caattgttct tcagcgatac ccgacagaag
      781 cagcggcaaa agtaccaaat aaacaacggt tgtcgaaaca taccaggcca tttgccagag
      841 gttgaccgac gttccggttt tcttcgacgt tgaaccacga agctctgcca cggcattggt
      901 tgccaaaggg aagtcgatgc ctaaaccaaa gcccatgatg acgcggcaca ccagcaccca
      961 ccagatactg ggagcaatag ctaccgctgc agcaccgatg gtgcacagcc ccatgccaat
     1021 aataaatgcc cgcttttgcc caaagcggtt tattactcgg ttcgctaata aaccgccaat
     1081 taacgcggcg attaatactg atgcattgac ggttgcagca agacccggcg aaatgccgaa
     1141 ttgagcggca atgtatttat taccaaaccc caccatcgcc gcctagtagg catcaatcaa
     1201 tatgccgccc aaagcaataa aaataacgcc gatactggtg ttgattgcag agttcttatt
     1261 aac
//
LOCUS       DAJVTY010000307         1240 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVTY010000307 DAJVTY010000000
VERSION     DAJVTY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.313"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            99..1115
                     /locus_tag="OQQ66_004135"
     CDS             99..1115
                     /locus_tag="OQQ66_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HCS1906822.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
BASE COUNT          346 a          327 c          329 g          238 t
ORIGIN      
        1 aaccgagaga gaacgaatga acgccctgag aatggtcatt ccgccacgat atccctggtc
       61 tctgatggaa ggtgcgaaca agtccctgat atgagatcat gtttgtcatc tggagccatg
      121 gaacagggtt catcatgagt catcaactta ccttcgccga cagtgaattc agcagtaagc
      181 gccgtcagac cagaaaagag attttcttgt cccgcatgga gcagattctg ccatggcaaa
      241 acatggtgga agtcatcgag ccgttttacc ccaaggctgg taatggccgg cgaccttatc
      301 cgctggaaac catgctacgc attcactgca tgcagcattg gtacaacctg agcgatggcg
      361 cgatggaaga tgctctgtac gaaatcgcct ccatgcgtct gtttgcccgg ttatccctgg
      421 atagcgcctt gcctgaccgc accaccatca tgaatttccg ccacctgctg gagcagcatc
      481 aactggcccg ccaattgttc aagaccatca atcgctggct ggccgaagca ggcgtcatga
      541 tgactcaagg caccttggtc gatgccacca tcattgaggc acccagctcg accaagaaca
      601 aagagcagca acgcgatccg gagatgcatc agaccaagaa aggcaatcag tggcactttg
      661 gcatgaaggc ccacattggt gtcgatgcca agagtggcct gacccacagc ctagtcacca
      721 ccgcggccaa cgagcatgac ctcaatcagc tgggtaatct gctgcatgga gaggagcaat
      781 ttgtctcagc cgatgccggc taccaagggg cgccacagcg cgaggagctg gccgaggtgg
      841 atgtggactg gctgatcgcc gagcgccccg gcaaggtaag aaccttgaaa cagcatccac
      901 gcaagagcaa aacggccatc aacatcgaat acatgaaagc cagcatccgg gccaaggtgg
      961 agcacccatt tcgcatcatc aagcgacagt tcggcttcgt gaaagccaga tacaaggggt
     1021 tgctgaaaaa cgataaccaa ctggcgatgt tattcacgct ggccaacctg tttcgggcgg
     1081 accaaatgat acgtcagtgg gagagatctc actaaaaact ggggataacg ccttaaatgg
     1141 cgaagaaacg gtctaaatag gctgattcaa ggcatttacg ggagaaaaaa tcggctcaaa
     1201 catgaagaaa tgaaatgact gagtcagccg agaagaattt
//
LOCUS       DAJVTY010000308         1229 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVTY010000308 DAJVTY010000000
VERSION     DAJVTY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.314"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>1229
                     /locus_tag="OQQ66_004136"
     CDS             <1..>1229
                     /locus_tag="OQQ66_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1906823.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADRLTHRTVNGETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEK
                     GRLTGERQTVHHPQPEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGM
                     KLGDTPLVDFTRDRLHR"
BASE COUNT          298 a          366 c          378 g          187 t
ORIGIN      
        1 gaacacgcgg acggcagcgt cacgcagagt cagtttgacg ccgtgggcag gctcaggaca
       61 cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac gggcctcatc
      121 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacagccag
      181 ttaacgtcag ccaccgggcc tgacgggctg gaaatacgcc gggaatatga tgaatggggc
      241 cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta tgataatcca
      301 cacagtgact taccctgcgc aacggaagat gccaccggta gccggaaaac catgacgtgg
      361 agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac ccgttatgac
      421 catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag tcagtaccgc
      481 gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca tgaaacgcgg
      541 tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      601 gggacacagt acgatgcgtg gggaaaggcc atccgtacca cgcagggcga gctgacgcgc
      661 agtatggaat acgatgctgc aggacgggtc atccgcctga ccagtgaaaa cggcagccac
      721 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      781 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      841 gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa
      901 accgcagagc agtggcagta tgacaaacgt ggctggctga cagacatcag ccatctcagc
      961 gaagggcacc gggtgacggt gcattacagg tatgatgaga aaggccggct gaccggtgag
     1021 cgccagacgg tgcatcaccc gcagccggaa gcactgctct ggcagcatga gaccagacat
     1081 gcgtacaacg cgcagggact ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa
     1141 tggctgacct acggcagcgg ctggctggca ggtatgaagc tcggcgacac accgctggtg
     1201 gatttcaccc gcgaccgcct gcaccggga
//
LOCUS       DAJVTY010000309         1172 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVTY010000309 DAJVTY010000000
VERSION     DAJVTY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.315"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OQQ66_004137"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OQQ66_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1906824.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          371 a          187 c          208 g          406 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcagaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gagctaaata gctgcgcgga atagtaggct gtgtccctta atcatctgag
     1081 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     1141 gaggcatgga ccatcatcaa gcccctgtta cc
//
LOCUS       DAJVTY010000310         1158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVTY010000310 DAJVTY010000000
VERSION     DAJVTY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.316"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(1..263)
                     /locus_tag="OQQ66_004138"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OQQ66_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OQQ66_004139"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OQQ66_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1906825.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          340 a          285 c          176 g          357 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt accaccaa
//
LOCUS       DAJVTY010000311         1148 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVTY010000311 DAJVTY010000000
VERSION     DAJVTY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.317"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1058
                     /locus_tag="OQQ66_004140"
     CDS             <1..1058
                     /locus_tag="OQQ66_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1906826.1"
                     /translation="KLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIPA
                     GVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLALL
                     AALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIGI
                     ARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFHD
                     RGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLGG
                     DLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIALW
                     TLIKVKDEEPQVQLATH"
BASE COUNT          266 a          254 c          287 g          341 t
ORIGIN      
        1 tgaagttaag ttttgcgata tgtgccgcaa tttggggcct ggcgacaatg ggccatgcac
       61 taaccggaac gtggtctggt ctggcattta tgcgcgccct gatgggtttc agcgaagcgt
      121 cggcaattcc ggcgggtgta aaaaccgcat caacatggtt cccggcaaaa gagcgtggcg
      181 tggcgacagg tgttttcaat atgggcacct cactcggcgc gatgcttgct ccaccgttga
      241 ttgcctggtg cattatgttt catagctggc aatttgcgtt tattgtttca ggtagtcttg
      301 ctttgctcgc ggctttattt tggttctttt gttataaaga tccgaaagat gccaaacgcc
      361 tttctgatga agagcgccac tatattgaat cagggcaaga acagcatctt aaaacagata
      421 agaaagaaaa aacgtcaatc aagcatatcc tcagccaacg taatttctgg gggattggca
      481 tcgcgcgttt tctcgcagac ccggcatggg gaaccattaa cttctgggtg ccgattttct
      541 tcgtcgaaac gctgcatttt agcctgaaag aaattgccat gttcgtctgg ctgcctttcc
      601 tgctgggcga tctcggctgt ttagccagtg gttttgtcgc gaagttcttc cacgatcgcg
      661 gcgtgagttt aattaactca cgaagaatta ccttcactat tgcagccgtc attatgatga
      721 cgattggcct ggtgagtatt gtcgaaaatc cctacattgc cgtattactg attagtattg
      781 gcgcgttctc gcatcaatgt ctttctactg tagcagcaac tctgggtggc gatctgttca
      841 aaaaagacga agtagctacc gcagtgggta tggcaggagc ctgtgcgtgg agcggtcagt
      901 tgattttcaa cctgttcatc ggggcattcg ttcacattat cggcttcgcg ccgttcttta
      961 ttgccctggc tttctttgac attattggtg ctattgcgtt gtggacgctt atcaaagtta
     1021 aagatgaaga accacaagta cagttagcta cccactaatt attttaaata aaaacgctat
     1081 aattgaggtt atggcgtttt taaagaagct tgagttatcg aaccccgctt accaacgcgt
     1141 tgtcagtc
//
LOCUS       DAJVTY010000312         1114 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVTY010000312 DAJVTY010000000
VERSION     DAJVTY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.318"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            15..173
                     /locus_tag="OQQ66_004141"
     CDS             15..173
                     /locus_tag="OQQ66_004141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906827.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
     gene            173..763
                     /locus_tag="OQQ66_004142"
     CDS             173..763
                     /locus_tag="OQQ66_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS1906828.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            750..>1114
                     /locus_tag="OQQ66_004143"
     CDS             750..>1114
                     /locus_tag="OQQ66_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906829.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDT"
BASE COUNT          282 a          243 c          328 g          261 t
ORIGIN      
        1 tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc catcagtttg tctgctggtg
       61 aagtggccca ccggttaatc gtcccttcat cggatattga cctgggggag accgagcttc
      121 cggtgctcgg cgagatcacc tggcaggctt atgacccggc aaggagtaaa taatggattc
      181 gttacaggat gattatacaa agctgctgta cggactgatg cagccggggc ctgcatggtc
      241 agataccgac ggtgtacttg acggtctggc accatcgctt gtgcgtgttc accagcgggc
      301 tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca gagctgattg aacgttatga
      361 agaattgtat ggtttacctg attcctgttc ccctgtcgga acccagacat tacgccagcg
      421 tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc ataaatgagc agttcttcct
      481 ggatcagctt gaggcgctgg gatataccgg cgtaacgatc gaacagttcc agcacctgga
      541 tgcaagcccc gatccggaat ggggagatcg ctggcgttat ttctggcgtg tgacgttgcc
      601 ggtggatgcc ggtgctcagt ggcagacatg cacggacgcc tgcaacacac cgatccggac
      661 gtggggggat acggttgcgg agtgcgtgat taataaatta tgtccgtcac acaccgtcgt
      721 tttattttcc tatccagatg aggatgaaga tgcacaggat tgatacgctg actgcggtaa
      781 aagataagtt tggtccgggt aagaacggat ttactgacgg aaatcttcgc acaggacgtc
      841 ttgctacctg gctgaacagt gccatgtgga atgccattca ggaggaaatc tgtggtgtca
      901 ttgagaaggc cgggatagaa ctgaataaag aagaacacga ccagctatat aaggctatat
      961 tattgctggt gggcggtgca attaacgaag aggcattgct gataaaaaat aacctttcgg
     1021 atgtggaaga cagggatgag gctgttgaaa acctcggatt aaaacccacg gtggataagg
     1081 caaaaaatgc cgttcagcgt gatggcgaca ccat
//
LOCUS       DAJVTY010000313         1110 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVTY010000313 DAJVTY010000000
VERSION     DAJVTY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.319"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            87..1006
                     /locus_tag="OQQ66_004144"
                     /pseudo
     CDS             87..1006
                     /locus_tag="OQQ66_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1006..>1110
                     /locus_tag="OQQ66_004145"
     CDS             1006..>1110
                     /locus_tag="OQQ66_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1906830.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAII"
BASE COUNT          323 a          254 c          259 g          274 t
ORIGIN      
        1 atttagggtc taatcaactt aatgatcaat atctatagtg atatcaccaa ccgttaaaac
       61 gaaccgacaa ctccatgagg aacggaatga tgaataataa agtcagcttc actaacagca
      121 ataatccaac catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc
      181 gtcagtatca ggcaattgtg gtatctcacc caggtggggc gttaaagaac aaacggccgg
      241 aacctatgcc aaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
      301 tcaaggtgaa agtggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
      361 catcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata cccgaattgg
      421 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
      481 catcaaggcc atcggtaccg ttagtgcggt caatattggt tcgatgttcc gtaatggttg
      541 ggaaaacaat gtgaaatcta ttgatgccct gccatacgtt gaagcgggtt caaacgcccg
      601 aaccagcgat attagcagcg gagaatatgc cgttatgcca ctggcaccaa tgaaggagtc
      661 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccacaccc ctcgcgcgca
      721 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctttga
      781 tgcttaccat atggcggaag tgtacctgac tcagccgatg cagattgtgg cgggcagcca
      841 ggcagggagc aaatggatga gtgacgatct gtacgatcga gcatcaagcc aagataaacg
      901 ctatcacatt gttgaaggcg caaaccatat ggatctttat gacggcaaag cctatgtggc
      961 tgaagctatt tcagtattag ctccgttctt tgaggaaact ctgtaatgaa acatattcaa
     1021 atccgcaata gtgatatggc ctggcatatt gctgccaata ttcagtttcc accaaatttt
     1081 gatgagtcaa aacagtatcc ggcgattatc
//
LOCUS       DAJVTY010000314         1102 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVTY010000314 DAJVTY010000000
VERSION     DAJVTY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.320"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..638)
                     /gene="ospC4"
                     /locus_tag="OQQ66_004146"
     CDS             complement(<1..638)
                     /gene="ospC4"
                     /locus_tag="OQQ66_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1906831.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGA"
BASE COUNT          311 a          188 c          207 g          396 t
ORIGIN      
        1 gcgccaaaat ctacagtatg atgtgtttta tttagcggga gtttttcttg atgattggaa
       61 atttcaaccc caaaaaaaac aaaatcgtgg ttagagattt ctttaatatc ctcagagtat
      121 gtattcttat gtgtttgtat attttcagac tgaattttat caaaggattt aatattaaga
      181 atattatttg acagattaga gttactttga tgtctgaagt taaaattaga attaataata
      241 atagaaagta agtgacgttc gtctgaagaa agaggatgaa ctttttgtat tatctcagag
      301 atatagtgcc tggacacctt atcgagatgc tcaagtgacc gaaatgatga ccgtgctcca
      361 gatggcgctt caatagaaag atttagtgat ttgaaactgg tcccttgaga aaacattttg
      421 ctataacttt gcgctgctat agacttcctt aaaaagtttt tcacagtatt agcacagtga
      481 tctaaatgtt tttcacttat gccagaagaa gatgagtttg ttacgcgtaa tatatttttt
      541 tgtaatgctt ttgttgcttt attcggattc gtttttccat caacaagatc aatattattc
      601 tgaacattaa tatgatttac tgcttcaggt attttcatcc cccccccaat tatgtgcgtt
      661 tttatctgtg gttatttaga taacattatc taactggaca ctttcttgta tgtattatag
      721 ctaaatgaac agattttaat atgttgtatg gttttcactc tgaaatcccc tgaatttaat
      781 cacgtttcct ttactgaagg caattttaac cgtagtatca attattaacc cttgggcttg
      841 atatccctct cctcttctgg gcacggggga tgtcagatta cttattacag aaaccaaaag
      901 aattgtaagc gtcatctcag tgtcgtctat ttcaatgtct gatgattggc gatgatagag
      961 tccttgaacc gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat
     1021 catcgtttcc gatggaagca taataagctt tttctgcttc tgccggagga gtatggccca
     1081 gcctttccag caatcgtcga tt
//
LOCUS       DAJVTY010000315         1099 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVTY010000315 DAJVTY010000000
VERSION     DAJVTY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.321"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..367
                     /locus_tag="OQQ66_004147"
     CDS             <1..367
                     /locus_tag="OQQ66_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1906832.1"
                     /translation="INAWDNEDFVKAVKATGKKQLIIAGVVTEVCVAFPALSAIEEGF
                     DVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHRDWHNDIEGLATLFSN
                     HIPDYRNLMTCYDTLTKQK"
     gene            complement(402..>1099)
                     /gene="dmsC"
                     /locus_tag="OQQ66_004148"
     CDS             complement(402..>1099)
                     /gene="dmsC"
                     /locus_tag="OQQ66_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS1906833.1"
                     /translation="IGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGI
                     GWLLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLT
                     MFMGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQA
                     AALVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVF
                     YGLHMTVGMAVAS"
BASE COUNT          308 a          296 c          277 g          218 t
ORIGIN      
        1 tattaacgcc tgggataacg aagattttgt aaaagctgtc aaagcgacag gtaaaaaaca
       61 gttaattatt gccggtgtgg taaccgaagt ttgcgtggca ttcccggcgc tttcagccat
      121 tgaagaaggg tttgacgtct ttgtcgtgac tgacgcgtca gggaccttta atgaaattac
      181 ccggcattcg gcatgggatc gcatgtcgca agctggtgcg caattgatga catggttcgg
      241 cgtggcgtgc gagctgcatc gcgactggca taatgatatt gaaggactgg cgacgttgtt
      301 ctctaaccac attcctgatt atcgtaattt gatgacctgt tatgacacgt taacgaagca
      361 gaaataaaag agaaccgggg ccgaaacccc ggttgttatg attagcttgc gacggccatc
      421 cccacggtca tatgcaaacc atagaatacg ccgcgaccga ttaattcccc tgccagaagc
      481 agaatgaatg atacagaaag tagcggaacc gcaggctgat aaccttttag ctgtggtgca
      541 atccacaagc acagggcaac ggccaaaagc acgatccgcc aggacatcag cgcaccatag
      601 tccggcacca gcgctgcggc ctgctgcaca gaactatgaa tagtcgccag ctctgcgccc
      661 tgcatcactg acaccacgcc actcactacc agtgccagta cagaaattgc tggcagcaga
      721 cgcatcgccc agccatcgac gccagccaga ctcaacaaca ggtaacccag taacgggccg
      781 cccataaaca tcgtcaggaa gaagcccatc ggcgtccaga tgctgtacca ggtcggaacg
      841 gtatcaatgc tgttatacac acgcaccatc atccagacaa agatgacgcc aagaaccatc
      901 gtcactatca gccacagcgt acgcaatgcc ggtgacagct ttttcagcat tgccagcaac
      961 cagccgatgc cgcctacggc aaaaaagatc gaaccgctgg cgatttcgtt actgagtgct
     1021 gaagccccta cccggttgag cgagttaaaa gcgcgcattg gtgaaccaag atggagcata
     1081 gaggcgataa agccaatgc
//
LOCUS       DAJVTY010000316         1056 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVTY010000316 DAJVTY010000000
VERSION     DAJVTY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.322"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..1033
                     /locus_tag="OQQ66_004149"
     rRNA            <1..1033
                     /locus_tag="OQQ66_004149"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          261 a          247 c          330 g          218 t
ORIGIN      
        1 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc
       61 gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga
      121 actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc
      181 ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga
      241 ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      301 ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg
      361 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
      421 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      481 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      541 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      601 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      661 aactggagga aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca
      721 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      781 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      841 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      901 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      961 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
     1021 gatcacctcc ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAJVTY010000317         1048 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVTY010000317 DAJVTY010000000
VERSION     DAJVTY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.323"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            35..313
                     /locus_tag="OQQ66_004150"
     CDS             35..313
                     /locus_tag="OQQ66_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1906834.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            300..848
                     /locus_tag="OQQ66_004151"
                     /pseudo
     CDS             300..848
                     /locus_tag="OQQ66_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          263 a          239 c          294 g          252 t
ORIGIN      
        1 ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat accggcggct
       61 gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata ttaaccggat
      121 gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg gtgggctgag
      181 gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta tccagtcgtc
      241 gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg agtcccgtaa
      301 tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc ggagataaaa
      361 accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct gcgtatgatt
      421 caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg ccacacggca
      481 aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc caggctggcg
      541 gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca aaaggagcca
      601 ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat aacctgaccg
      661 atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg aaaaggatat
      721 tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta cgggataatc
      781 ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa ggcgataaaa
      841 taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac gaagcttttg
      901 gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt gaaggtcagg
      961 gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatctgcgg acgggtgaaa
     1021 tatccatgtc gcataaagtg tctgttgg
//
LOCUS       DAJVTY010000318         1041 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVTY010000318 DAJVTY010000000
VERSION     DAJVTY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.324"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..430
                     /locus_tag="OQQ66_004152"
     CDS             <1..430
                     /locus_tag="OQQ66_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1906835.1"
                     /translation="PKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRP
                     DGLKGNPSEAPLALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMI
                     APFSDEVMKVTGLNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            465..1041
                     /locus_tag="OQQ66_004153"
                     /pseudo
     CDS             465..1041
                     /locus_tag="OQQ66_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="similar to the fimbrial usher protein PapC;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
BASE COUNT          316 a          215 c          223 g          287 t
ORIGIN      
        1 gcctaaagac agagagagcg tgttctggtt taacgtactg gaagttccac caaaaccaga
       61 tgcagaaaag gtcgcgaatc aaagtctgct gcaactggca tttcgcacac gtataaaact
      121 tttctatcgc ccggatggat tgaagggaaa tccctctgaa gccccgttag ccctgaagtg
      181 gttctggtca ggttcagaag gtaaggcgtc attgcgagtg accaatccaa ccccttacta
      241 cgtctctttt agcagtggtg atttagaagc tagcggtaaa cgctatccga ttgatgtgaa
      301 aatgattgca ccatttagtg atgaggtcat gaaagtcact ggccttaatg gcaaagcaag
      361 ttctgcaaaa gtacattttt acgccattaa tgattttggt ggcaccattg aaggtaatgc
      421 cagtctgtaa tcaggcaagg atataattcc gcaggaagca taacgtgact ataaaaccaa
      481 ctaaaaatta tcataatcat ctgacccgta tcgctacgtt ttgcgcgctg ctgtattgca
      541 atagtgcttt ctgtgctgaa cttgttgaat atgaccatac cttcctgatg gggcagaatg
      601 cctcaaatat tgatctcagc cggtatagtg aaggtaaccc tgctataccg ggtatgtacg
      661 acgtcagtgt ttatgtcaac gatcaaccta ttattaacca aagcattaca tttattgaaa
      721 ttgaaggaaa aaaaacgcac aagcctgtat cacacttaag aatttattgc agtttcatat
      781 aaattctccc gatataaata acgaaaaagc cgttctgctt gccagggatg aaacactcgg
      841 caattgcctt aatttgacgg aaattattcc tcaggcttct gttcgttatg acgttaacga
      901 gcaacgtctg gatatagacg ttcctcaggc atgggtaatg aaaaattacc aaaactatgt
      961 tgatccatcg ttatgggaaa atggcattaa tgcggccatg ttgtcataca acctcaacgg
     1021 atatcatagt gaaactcctg g
//
LOCUS       DAJVTY010000319         1009 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVTY010000319 DAJVTY010000000
VERSION     DAJVTY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.325"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQQ66_004154"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQQ66_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1906836.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQQ66_004155"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQQ66_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1906837.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLEQEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1009)
                     /locus_tag="OQQ66_004156"
     CDS             complement(941..>1009)
                     /locus_tag="OQQ66_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1906838.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          302 a          205 c          231 g          271 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac aggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaagg
//
LOCUS       DAJVTY010000320          999 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVTY010000320 DAJVTY010000000
VERSION     DAJVTY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.326"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..116
                     /locus_tag="OQQ66_004157"
     CDS             <1..116
                     /locus_tag="OQQ66_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1906839.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OQQ66_004158"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OQQ66_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1906840.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..>999
                     /locus_tag="OQQ66_004159"
     CDS             494..>999
                     /locus_tag="OQQ66_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906841.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVK"
BASE COUNT          254 a          255 c          284 g          206 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaa
//
LOCUS       DAJVTY010000321          985 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVTY010000321 DAJVTY010000000
VERSION     DAJVTY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.327"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..94
                     /locus_tag="OQQ66_004160"
     CDS             <1..94
                     /locus_tag="OQQ66_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1906842.1"
                     /translation="NYIECDYNRWRWYSWCGGLSPEQFENQNLT"
     gene            434..691
                     /locus_tag="OQQ66_004161"
     CDS             434..691
                     /locus_tag="OQQ66_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCS1906843.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          316 a          171 c          187 g          311 t
ORIGIN      
        1 taattatatc gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag
       61 tccggaacaa tttgaaaatc agaatctcac ttaaaacggc atccatttta caaaagatgg
      121 ccgtctgtgt atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag
      181 gcgatatcct aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa
      241 aaaagagttg actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa
      301 aaaaaaccat tagtctgctt cctgttctta caataatcag tattatgtat tttatttata
      361 tagcaaaata tgcttattac acctgtcata attaggcatg gtaaaattat tgactgaacc
      421 ctgggggaga aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa
      481 tattttatat gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt
      541 caacgcttta ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat
      601 gttctttttg tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat
      661 tatcattgac attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg
      721 agtcttatct gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt
      781 tgaatcggtt acgtaccccg gagctggtca tattttcagg taatacacac gctttcagac
      841 atttcggctt tcgtcatttt ggtatcatgg gcatagcttc tgctacctga gcatcataat
      901 cacgcagctt cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat
      961 ccagcaataa tggacacgcg gctaa
//
LOCUS       DAJVTY010000322          976 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVTY010000322 DAJVTY010000000
VERSION     DAJVTY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.328"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..697
                     /locus_tag="OQQ66_004162"
     CDS             <1..697
                     /locus_tag="OQQ66_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HCS1906844.1"
                     /translation="VDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRYN
                     NRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSR
                     EDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMII
                     PLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKL
                     LKTRKSNEADPYP"
     gene            complement(851..>976)
                     /locus_tag="OQQ66_004163"
     CDS             complement(851..>976)
                     /locus_tag="OQQ66_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCS1906845.1"
                     /translation="LHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          272 a          199 c          231 g          274 t
ORIGIN      
        1 tgtcgacaaa atgtttgcca cagcattgca atccatagtg atgatggcag aactggcaat
       61 tcgtggtccg gaaaagttcc agaaacgagt gttctggaaa ggcctcgaaa ttcttgagga
      121 aattcgatac aataacagaa atgtgatttt tctggttccc catggctgga gcgtggatat
      181 tcctgcaatg ttgctggcag ctcaggggga aaaaatggcc gccatgtttc atcagcaacg
      241 aaatccagtg attgattatg tctggaattc tgtacggcgt aaattcgggg ggcgcttaca
      301 ttcccgggag gatgggataa aaccatttat tcagtcagta cgccagggat actgggggta
      361 ttaccttcca gatcaggatc atggtcctga atacagtgaa tttgctgatt tttttgcgac
      421 ctataaagcg acattaccaa ttattggacg tctgatgaac atcagtcagg ctatgattat
      481 accgcttttc ccggtttatg atgaaaaaaa acatttcctg actattgaag ttcggccacc
      541 aatggatgca tgcattgcca gcgcggacaa taaaatgatt gcccgacaaa tgaacaaaac
      601 agtggaaatt ttggtggggt cacatccgga acagtatatc tgggttttaa aattgttaaa
      661 aacgcgcaaa tcaaacgaag cggacccgta cccttgaaat tataaagtac aggtatttcc
      721 actagttgtt tcttacaggt taccaatcga aacacatccc ccttccgtgt gttgcccgtt
      781 tggcacggag gactgtgtag atgcagttcg ttaaatttaa tttatgccta aatagctgcg
      841 cttaataccg ctacactttt gccagtccat gtttgcctcc ggggaatggg ctgacggttt
      901 ccataaaatg gcgaactttt ttcaacagtt gccacattga gcggcactga tgattacgcg
      961 ttattgtgtc gtgaag
//
LOCUS       DAJVTY010000323          966 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVTY010000323 DAJVTY010000000
VERSION     DAJVTY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.329"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            122..574
                     /gene="yeeE"
                     /locus_tag="OQQ66_004164"
                     /pseudo
     CDS             122..574
                     /gene="yeeE"
                     /locus_tag="OQQ66_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            588..815
                     /locus_tag="OQQ66_004165"
     CDS             588..815
                     /locus_tag="OQQ66_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1906846.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
BASE COUNT          225 a          220 c          272 g          249 t
ORIGIN      
        1 cattataacg aatggcatcc gcatagtgcg ctgggttatc gctcgccacg ggaatatctg
       61 cggcagcggg cttgtaatgg gttaagtgat aacagatgtc tggaaatata ggggcaaatc
      121 cactgctgta ctcatcggtt tgattgcgct tttagcctgg cccctgagtg aagcaaccgg
      181 acgcatgttt gggttaggaa tcacttcccc aacggccaat attctgcaat ttctggtcgc
      241 gggtgacgtg aaatacatta actggggcgt tttcctggtg ttagggatct tcgtggggtc
      301 atttattgca gccaaagcga gccgtgagtt ccgcgttcgc gcagctgatg cacaaacaac
      361 attacgtagc gggttaggtg gtgtactgat gggcttcggt gccagtattg caggtggttg
      421 ctctatcggt aatggactgg ttatgactgc aatgatgacc tggcagggct ggattggcct
      481 tgtatttatg attctcggag tctggactgc atcctggctt gtgtatgttc gaccgcagcg
      541 taaggcgcga ctggcgactg ctgcggcaaa ttaagtaagg tgtaaagatg gcgattaaaa
      601 agctggatgt ggtgacgcag gtctgtccgt ttccactcat agaagcaaaa gcagcactgg
      661 cagaaatggc cagcggcgat gagttggtaa ttgagtttga ttgcacccag gcaactgaag
      721 caatcccaca gtgggcggca gaagaaggac atgccatcac cgattatcag cagattggcg
      781 atgcagcgtg gagcatcacc gttcaaaaag cctgactcaa cattgtcctc cgccgtacca
      841 gcggcggagg cttcaaatta gacgatctct tccgcgtact gccaaagtgc ttttaacagc
      901 gccacttttt ctttgtcatc ggcatattgt tgtaccagca tctgcaattc atcagcataa
      961 ccctgc
//
LOCUS       DAJVTY010000324          960 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVTY010000324 DAJVTY010000000
VERSION     DAJVTY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.330"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            199..>960
                     /locus_tag="OQQ66_004166"
     CDS             199..>960
                     /locus_tag="OQQ66_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1906847.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPL"
BASE COUNT          329 a          223 c          140 g          268 t
ORIGIN      
        1 cattaaaccg atccagttta ggatagtgta aagcagaagt tcttcaatat ataacatatg
       61 gagttaatat gcttacacaa actatatatc cgcctcctcc agaagttcct gtctactgct
      121 gaagggaacc aataacattg caaagcagat aaaaaagaat ccaacacctc tcaacataaa
      181 gaatacgccc tgatatttat gctcccgaca aataacaatc acagattaat ttcaaattcg
      241 ttctccactt attcaatcga cactagccgc gcatatgaaa attatctaac ccattggact
      301 gaatggaaaa ataaccgcat acaagaagaa caacgagaca tcgcttttca gcgactagta
      361 tcatgtctac aaaaccaaga gacgaaccta gacttgtctg aattaggcct gacaacatta
      421 cctgaaatcc ccccgggaat taaatcaatt aatataagta aaaataattt aagcttaatc
      481 tccccattgc ctgcgtccct tacacagctt aatgtcagct ataacagact tattgaactg
      541 cctgctttgc ctcaaggact taaattattg aatgcgtccc acaatcaact aatcacacta
      601 cccacactcc ccatatcttt gaaggagctt catgtctcaa ataatcaatt atgttctctt
      661 cctgttttac cagaactact ggaaacatta gatgtatcat gtaatgggct ggcagtttta
      721 ccacctttac cattttcttt acaagagatt agcgcaatag ggaatcttct tagtgaactc
      781 ccccctctac ctcacaacat tcactccata tgggcaatcg acaatatgtt aaccgatatt
      841 ccatacctgc cggaaaattt aaggaacggt tattttgaca taaatcagat aagtcatatc
      901 ccggaaagca ttcttaatct gaggaatgaa tgttcaatag atattagtga taacccattg
//
LOCUS       DAJVTY010000325          949 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVTY010000325 DAJVTY010000000
VERSION     DAJVTY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.331"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..534
                     /locus_tag="OQQ66_004167"
                     /pseudo
     CDS             1..534
                     /locus_tag="OQQ66_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          278 a          215 c          203 g          253 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 gaaaattgta caacatattg aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttaatat tctaaataac catagataaa aatgcacata atggggaata ttttatgaaa
      901 atacctgaag cagtaaatca tattaatgtt cagaataata ttgatcttg
//
LOCUS       DAJVTY010000326          944 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVTY010000326 DAJVTY010000000
VERSION     DAJVTY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.332"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>944
                     /gene="pdeB"
                     /locus_tag="OQQ66_004168"
     CDS             <1..>944
                     /gene="pdeB"
                     /locus_tag="OQQ66_004168"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1906848.1"
                     /translation="HLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPG
                     KISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGN
                     AHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQK
                     GWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLA
                     NGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQH
                     PQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIA"
BASE COUNT          238 a          230 c          220 g          256 t
ORIGIN      
        1 cccatctcat ggaaatgcgt cgggtatctt acagttatcg ctatattcag gaagtggttt
       61 atatcgataa caacgttccc cagtgttcgt ctctggagca tgaaagtccg cccgatacct
      121 tccccgagcc aggtaaaatt tcgaaagatg gttatcgtgt ctggttaaca tcgcataacg
      181 atttaggcat tatccgttac atggtcgcca tgggaacggc acattatgtc gtcatgatcg
      241 accccgcttc ctttattgat gtcattccct atagctcatg gcaaattgat gccgccatta
      301 ttggcaatgc ccataacgtt gtcataacca gcagcgatga aattgctcag ggaattatta
      361 ccaggctaca aaaaacaccc ggtgagcata tcgaaaataa tggaatcatt tacgatatcc
      421 tgcccttccc ggagatgaat atttcgatca tcacctgggc ttcaacgaaa atgttgcaga
      481 aaggctggca tcggcaagtc tttatttggt taccgctcgg gttggtgatt ggcctgctgg
      541 cagcgatgtt tgtgctgcgt attttgcgcc gtatccagtc accgcatcat cggctgcagg
      601 atgctatcga aaatcgtgat atttgcgtgc actatcagcc gattgtctcc ttagccaatg
      661 gcaaaattgt cggtgctgag gcactggcgc gctggccgca gacagacggt agttggttgt
      721 caccagatag ttttattccg ctggcacagc aaacaggcct ttctgagcca ttgacgctac
      781 tgattataag aagcgtcttt gaagatatgg gcgactggct gcgtcagcat ccacagcagc
      841 atatttcgat caatcttgaa tccaccgtgc tcacctcgga aaaaatcccg caattgctgc
      901 gtgaaatgat caatcactat caggttaatc ccagacagat cgcg
//
LOCUS       DAJVTY010000327          934 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVTY010000327 DAJVTY010000000
VERSION     DAJVTY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.333"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            28..603
                     /locus_tag="OQQ66_004169"
     CDS             28..603
                     /locus_tag="OQQ66_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1906849.1"
                     /translation="MRWRHPRLGLLPPGQFLPLAESFGLMPEIGAWVLGEACRQMHKW
                     QGPAWQPFRLAINVSASQVGPTFDDEVKRVLADMALPAELLEIELTESVAFGNPALFA
                     SFDALRAIGVRFAADDFGTGYSCLQHLKCCPITTLKIDQSFVARLPDDARDQTIVRAV
                     IQLAHGLGMDVIFRRRLHQLIGRNGCCAASS"
     gene            678..>934
                     /locus_tag="OQQ66_004170"
     CDS             678..>934
                     /locus_tag="OQQ66_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906850.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKG"
BASE COUNT          168 a          282 c          302 g          182 t
ORIGIN      
        1 caccggatcg ctggcgcgga agcgttgatg cgctggcggc atccgaggct tggcctgttg
       61 ccgcccggcc agttcctgcc gctggccgag tcgttcggcc tgatgccgga aataggcgcg
      121 tgggtgctgg gcgaggcctg tcgccagatg cacaagtggc aaggaccggc atggcaaccg
      181 ttccgtcttg ccatcaatgt gtccgccagc caggttgggc caacgttcga cgacgaggta
      241 aagcgggtgc tggccgatat ggccctgccc gccgagcttc tggagatcga actgaccgaa
      301 tcggtcgcat tcggcaatcc agccctgttc gccagtttcg acgccttgcg cgccatcggc
      361 gtgcgcttcg ccgccgacga cttcggcacc ggctattcct gcctgcaaca tctgaaatgc
      421 tgccccatca ccacattgaa aatcgaccaa tcctttgtcg ccaggctccc ggatgatgcc
      481 cgtgaccaaa ctatcgtgcg ggcggtgatc cagctcgcgc acgggctggg catggatgtc
      541 attttcagaa gacgactgca ccagttgatt gggcgtaatg gctgttgtgc agccagctcc
      601 tgacagttca atatcagaag tgatctgcac caatctcgac tatgctcaat actcgtgtgc
      661 accaaagcga ggtgagcatg gcgacggaca ccccacggat tccagaacaa ggcgtggcca
      721 ctctgcctga tgaggcttgg gagcgtgcgc gccgtcgtgc ggagatcatc agtccgttgg
      781 cgcagtcgga gacggtcggg cacgaagcgg ccgatatggc ggctcaggcg ctgggcttgt
      841 ctcggcgcca ggtatacgtt ctgatccggc gtgcccggca aggcagcggc ctcgtgacgg
      901 atctggtgcc cggccagtcc ggtggaggta aagg
//
LOCUS       DAJVTY010000328          931 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVTY010000328 DAJVTY010000000
VERSION     DAJVTY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.334"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(48..384)
                     /locus_tag="OQQ66_004171"
                     /pseudo
     CDS             complement(48..384)
                     /locus_tag="OQQ66_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          307 a          167 c          168 g          289 t
ORIGIN      
        1 ataaatatga aaagatggtt taaatacccc gttacttttg acttacacta tacggtatcg
       61 catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac aatactgaat
      121 gcgtacgacc acagtattct ggctcctgtg tggtcatgct ttaattctgc gttccggaca
      181 gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct ttttaccagt
      241 gctgatttcc acattccgtc caacagagct tatagccttt ccctggatta tagcattgtc
      301 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
      361 ataacccact gtactgataa ccataatcta atcagtagaa attgagtcga aaataagcac
      421 tactccatac aggataatta gaggtcagtt tgattattca caattcatta tcagcatttt
      481 ctgtttctga cgaaatcaac atgaaaagaa ccatatatga taattattat aataacggct
      541 ttaattggaa tacatatatt acaacgtatt atatataatt ggtattctgg gaactatatt
      601 ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa ggacagtggg attaaaaagt
      661 aagaattgat aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
      721 aaaatgtgat gcctagagca ccatgaatat ataattaaaa atagttagag ttgtcaatac
      781 tttcacaaaa ataattttcg tagtttcgta gtatagaaag atatttttat gcatgaccta
      841 cctgaatttg ctccgggtag aggttataaa taaaaattga atcacgacaa acacaatatt
      901 catagtatgg cgatgcctac gccagcaaga a
//
LOCUS       DAJVTY010000329          929 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVTY010000329 DAJVTY010000000
VERSION     DAJVTY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.335"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(117..389)
                     /locus_tag="OQQ66_004172"
     CDS             complement(117..389)
                     /locus_tag="OQQ66_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1906851.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            complement(400..810)
                     /locus_tag="OQQ66_004173"
     CDS             complement(400..810)
                     /locus_tag="OQQ66_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1906852.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
BASE COUNT          153 a          295 c          222 g          259 t
ORIGIN      
        1 cctctgttgt gtctgtttgt ttcagatagt aactatgcct gaccgtgacg aaaacccggt
       61 aatgcgccat accgtttcaa ctggtgcaaa aaagccggat ttctccggct gtttgattag
      121 ctgtcctggt aattgcgcca tatttcatca ccagcaccat ctatacccat ttcggcataa
      181 gtgcgatcga ctgccttttt caggtctccg aaattatccg gcggctccgg tgccctctgt
      241 gccttccttg aacattccag ccgtcgcatc gtgatgtgat gccgttcctt gtctgtctcc
      301 accagctgca tgacttcacc ccatcgcgcc gccgccctcc ggtaaaagcc tttcgcctcc
      361 agttcctccg ctatgcggtc atgtaccatc gtcaccccct cagaacgaaa tatcatcacc
      421 gtaagggtca tcgcctcccg ctggtggctg attaccctgt gtgcctgtgg ttttgcgtct
      481 gttcccgcct ggacgtgccg cgcgggcact gattacgctg tctgcgataa cctgccagcc
      541 ctgccgcgtt tctccgttct gtccggtcca ctggcttacc tgcatcgtgc cggatacgct
      601 ggcaacatcg cctttttgat gtttagccag gaagtcggcc tgtttgccaa atgcgatgac
      661 cgataaccat aacgtcgcct gtccgtcctg tgcctggctg catggcaaag ataccgccat
      721 acgcgccagc gtcatcggtg tgcccttgct ggtctgtttt acctgcgggt cgtccaccag
      781 ccgcccgtaa gcggctatct gtgccgtcat aattccacct ctccggtttt aacgttgatg
      841 gttgttacct gttccgcttc ggcaatctcc cgttctgtca gcgtggcaaa gtttgccgcc
      901 gccgtggtca tgaatgcgct tatcaggtc
//
LOCUS       DAJVTY010000330          919 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVTY010000330 DAJVTY010000000
VERSION     DAJVTY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.336"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..294
                     /locus_tag="OQQ66_004174"
     CDS             <1..294
                     /locus_tag="OQQ66_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1906853.1"
                     /translation="KLGVKDEPLTLLKNVRGLELLTFAEQDTCCGFGGTFSVKMAEIS
                     GEMVKEKVGHLMEVRPEYLIGADVSCLLNISGRLQREGQKVKVMHIAEVLMSR"
     gene            305..>919
                     /locus_tag="OQQ66_004175"
     CDS             305..>919
                     /locus_tag="OQQ66_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HCS1906854.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITG"
BASE COUNT          258 a          195 c          262 g          204 t
ORIGIN      
        1 aagctgggag tgaaggacga gccacttacg ctgctgaaaa atgtgcgtgg actggagctg
       61 ttgacctttg ctgaacagga tacctgctgc ggatttggcg gcacgttctc ggtcaaaatg
      121 gccgaaatat ccggcgagat ggtgaaagaa aaggttgggc acctgatgga agtccgccct
      181 gagtatttaa ttggtgctga cgtgagttgc ctgctgaaca tcagtgggcg attacaacgg
      241 gaagggcaga aagtcaaagt gatgcatatt gctgaagtgt tgatgagccg ctgaggatat
      301 aaagatgtcg atcaaaacca gtaatacaga ttttaagact cgcatccgtc agcaaattga
      361 agatccgatc atgcgcaaag cggtggcaaa cgcgcagcag cgtattgggg caaatcggca
      421 aaaaatggtc gatgaattgg ggcactggga ggagtggcgc gatcgggccg cccagatacg
      481 tgatcatgtt ctgagtaatc tcgacgctta tctgtaccag ctctcagaaa aagtgacgca
      541 aaacggcggt cacgtctggt ttgcaaaaaa caaagaagac gctacccgct acattttaca
      601 ggttgcccaa cgcaaaaatg cccggaaggt ggtgaaatct aaatcgatgg tgaccgaaga
      661 gattggtgtc aatcatgtgt tgcaggatgc tggcattcag gtgattgaaa ccgatctggg
      721 tgaatatatt ctccagctgg atcaagatcc gccatctcat gttgtggtcc cggcaattca
      781 taaagatcgc catcagatcc gtcgagtgct acacgaacgt ctgggctatg aggggccgga
      841 aacgcctgaa gcgatgacct tattcatccg gcaaaaaatc cgcgaagatt tcctcagtgc
      901 tgaaataggt attaccggc
//
LOCUS       DAJVTY010000331          901 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVTY010000331 DAJVTY010000000
VERSION     DAJVTY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.337"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(116..388)
                     /locus_tag="OQQ66_004176"
     CDS             complement(116..388)
                     /locus_tag="OQQ66_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1906855.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            complement(399..809)
                     /locus_tag="OQQ66_004177"
     CDS             complement(399..809)
                     /locus_tag="OQQ66_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1906856.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISV
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
BASE COUNT          155 a          288 c          210 g          248 t
ORIGIN      
        1 ctctgttgtg tctgtttgtt tcagatagta actatgcctg accgtgacga aaacccggta
       61 atgcgccata ccgtttcaac tggtgcaaaa aaagccggat ttctccggct gttgattagc
      121 tgtctgggta attacgccat atttcatcgc ttacaccatc tatacccatt tcagcataag
      181 tgcgatctac tgcctttctc aggtctccga agttatccgg cggctccggt ggcttctgtg
      241 ccttcctgga acattccagc cgtcgcatcg taacctgatg ccgttccttg tctgtctcca
      301 ccagcagcat gacttcaccc catcgcgccg ccgccctccg gtaaaagcct ttcgcctcga
      361 gttcttccgc tatgcggtca tgtaccatcg ccaccccctc agaacggaat accgtcaccg
      421 taagggtcat cgcctcccgc tggtggctga ttaccctgtg tgcctgtggt tttgcgtctg
      481 ttcccgccag gacgtgccgc acggacactg attacgctgt ctgcaataac ctgataaccc
      541 tgccgcgttt ccccgttctg tccggtccac tggctgacct gcatcgtgcc ggatacgctg
      601 gcaacgtcgc ctttttgatg tttagccagg aagtcggcct gcttaccaaa tgcgatgacc
      661 gatagccata acgtcgcctg cccatcctgc gcctgactac atggcaacga taccgccata
      721 cgcgccagcg tcatcggtgt gcccttactg gtctgtttta cctgcgggtc gtccaccagc
      781 cgcccgtaag ccgctatctg tgctgtcatg attccacctc tccggtttta acgttgatgg
      841 ttgttacctg ttctgcttct gcgatctccc gttctgtcag cgtggcaaag tttgccgctg
      901 c
//
LOCUS       DAJVTY010000332          900 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVTY010000332 DAJVTY010000000
VERSION     DAJVTY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.338"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..227)
                     /locus_tag="OQQ66_004178"
     CDS             complement(<1..227)
                     /locus_tag="OQQ66_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1906857.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            304..711
                     /locus_tag="OQQ66_004179"
     CDS             304..711
                     /locus_tag="OQQ66_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1906858.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          272 a          222 c          175 g          231 t
ORIGIN      
        1 catgcctgat tttcatgaat caccttccca ggtcgttttg ccttacgata ttcgtcataa
       61 actttggggt cgtactgaag ttccccgcca gatgcctctt gcaggcgcat cgcgcggcct
      121 tcaggaacca gttcccccca tgcagcaatg cttgccagcc taactcctgc ggcattggca
      181 agctttgttt tgctgccaaa aaacgctata gcatcaattt tcaacatatc gagctcctta
      241 gattttccta aggaaactag atcgtagaga aacctaagtc aagaaagatt agaattccct
      301 aatatgaaaa acgaaacctt cggtgctcgc ctcttacata ggcgtaaaaa attaaaactg
      361 tctcaggccg cattaggtaa gctggtcaaa gtggctcacg taacaatttc tcaatgggaa
      421 agagatgaaa cacagccggc ggggaaaaga ttattcgcac tgagccaagc gcttcagtgc
      481 tcaccgactt ggcttctttt tggggatgaa gataaacaac caggcgaacc gatcccggat
      541 aatcagccag ccattctgac agaagatcaa aaagagttac ttcaactgtt cgacgcactg
      601 cctgagtcag agcaaaaggc cctgttgtca gagatgcgtg ctcgagttga aaatttcaac
      661 aaactttttg aagaactact caaagctcgc aaaagaagcg caaacaaata atcccctttt
      721 ttctccacac cctgtaataa aaagcacaaa ctttcaaaca cttgtgtttt ttacatcaaa
      781 aaaacttagg tttttataca taaaatcttg actccacgtc ttaggttatt ctaaatttca
      841 ttcatcaaga caccgcacgg tgttctcagc aaacagttcc actaccccgg cgttaagggg
//
LOCUS       DAJVTY010000333          895 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVTY010000333 DAJVTY010000000
VERSION     DAJVTY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.339"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>895)
                     /locus_tag="OQQ66_004180"
     CDS             complement(<1..>895)
                     /locus_tag="OQQ66_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCS1906859.1"
                     /translation="SLDRLDDDVRAYLENNDALAPSPIERLQRMNPLSISLYKMHGYD
                     LTTQPLQFAMNNQHMNGGIEVDIWGQTSLPGCFAVGEVAGTHGVTRPSGAALNAGQVF
                     AVRLARFIGCTQKRSVEGDVAQLVASSLNIVREVIAQALANENGMALVTVKDKIQARM
                     SDYAGFICHADKVRHATRDALLLSEFVQQHGLTINHAGEIAELFMWRHMTLTSAAVLT
                     QLTHYIDAGGGSRGARMVIDPQGKCLPQTRRGAKEEWRFRSERAEDKNHRLTIQYSQG
                     SFITEVKSLRMQPCIKGIYFEK"
BASE COUNT          224 a          231 c          211 g          229 t
ORIGIN      
        1 tttttcaaag taaatacctt taatacacgg ttgcatacgt aaagacttca cttcggtaat
       61 aaaagaacct tgcgaatatt gaatcgttaa tctgtgattt ttgtcttcag cacgttcaga
      121 gcgaaaacgc cattcttctt ttgcgccgcg acgagtttgt gggaggcatt tgccttgtgg
      181 atcaataacc atccgtgctc cacgactgcc accacctgcg tcaatataat gcgtcaattg
      241 cgttaatacg gcggcagaag tcagcgtcat atgccgccac ataaacagtt cggcaatttc
      301 gcctgcgtga ttgatggtta aaccgtgttg ttgtacaaac tcacttagca gcagggcgtc
      361 acgggtggca tgacgaactt tatcggcatg acaaataaat ccggcgtaat cagacattcg
      421 cgcctggatt ttgtctttca ccgttaccag cgccattccg ttctcgtttg ccagtgcctg
      481 agcgataact tctctgacta tattcagaga tgaggctacc agctgcgcga catcaccctc
      541 aacagaccgc ttttgcgtgc aaccaataaa acgtgccaga cgaacagcaa aaacctgccc
      601 ggcattcaat gctgcaccac tagggcgagt gacgccgtgt gtgccagcaa cttcccccac
      661 ggcgaaacag ccgggcaggg aggtttgtcc ccagatatcc acttcaatgc cgccattcat
      721 atgctgatta ttcatggcaa actgcaaagg ctgtgtggtg agatcgtaac cgtgcatctt
      781 atacagcgag atagacagcg gattcattcg ttgcagccgt tcgatgggcg atggtgccag
      841 agcgtcgtta ttttccagat acgcgcgaac gtcgtcgtcc agtcgatcta atgag
//
LOCUS       DAJVTY010000334          892 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVTY010000334 DAJVTY010000000
VERSION     DAJVTY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.340"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
BASE COUNT          250 a          169 c          187 g          286 t
ORIGIN      
        1 acgtttaata gggacaatca ccatttggat tgtccgctga ggaggcgaac gttttcctag
       61 acagctttat catgtcagcc cacattgttg gcgctgacaa tactaaggac aaacgatggg
      121 gaagtagctt tttagcgtta ttttcgggag taaaaaacat ggtatctttc cgtatgtcag
      181 ttgtatcttg ctggtattcg tagtcgtgat aaggcataca acgatattta tcttcacgtt
      241 agtgaagaat aagttgtttt gtgatctatg ttaatctggg attacaatcc gggcttgata
      301 catcgcatca cgacttttat tctcttcaga atagaatatg ttactccccg ttttacaggc
      361 tcaggatttg aattacaaat tatactttta agtgaacaat aactaggggt atttcttatt
      421 tcctgaaacc aggcttggcc catttcggga tgagtttgaa aaaactcttg acgcatacga
      481 tagtgatgaa ataaacgaaa caaattacgt caacaaactc aggcgtcttg cctgacatgc
      541 tcctgacatc attgatattc acgctcatct ggcatatgca tttgcgctct ttatgctgca
      601 attctggcga atgttcattt acagcgtcat caggatgccg ttatactcat tgataaaata
      661 ctaacatata accctgagaa taatcagggg acacgctggt tactggggac ggaattgtta
      721 cgtgctggca agcatgacag ggctctgtac gtattgacag gacatgtcaa tgaatttccc
      781 ccctactggt acgagcttgc tcttttacat tttatgaatg aggcatatat taatgctgca
      841 acggcatttc gatatggatt tttaacaagc atatattgtt gaaatgcttt gt
//
LOCUS       DAJVTY010000335          879 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVTY010000335 DAJVTY010000000
VERSION     DAJVTY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.341"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..351
                     /locus_tag="OQQ66_004181"
     CDS             <1..351
                     /locus_tag="OQQ66_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906860.1"
                     /translation="NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPES
                     NGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQ
                     RACNGLSDNRCLEI"
     gene            599..879
                     /locus_tag="OQQ66_004182"
                     /pseudo
     CDS             599..879
                     /locus_tag="OQQ66_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          234 a          217 c          234 g          194 t
ORIGIN      
        1 aacgatcttc cgtcgtctcc agtggagtgg ctgacggata atggttcatg ctaccgggct
       61 aatgaaacac gccagttcgc ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg
      121 agtccggaga gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg tgactacatc
      181 agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga accttgcaga ggcgttcgag
      241 cattataacg aatggcatcc gcatagtgcg ctgggttatc gctcgccacg ggaatatctg
      301 cggcagcggg cttgtaatgg gttaagtgat aacagatgtc tggaaatata ggggcaaatc
      361 cacatccccg ccagcccggc taagatggag gcacggcaca gcccgcacac gacgctgagg
      421 ctacagagct gaaaacccac aaggccatgc cccgcatggc cgggtaaagc ggagccggcg
      481 gcgacttcct ttgtgctctg tccgggaccg gcaggaaaaa ggcggggata atgagatggt
      541 cacccccacc ctaacagcag tacgctgtta aggtgaatcc cttaacggag agaccatcat
      601 acagaacatc acacttattg gtatcgatct cggaaagcat tccttccaca ttcattgtca
      661 agacaagtct ggtaaggctc ttctgcgtaa aaaatttacc cagtactaaa ctgatgcagt
      721 ttctggcaac atgtccgccc tcagttgttg taatggaagc ctgtgcgggt gcacactttt
      781 tggctcgccg tatctctgaa ttttggacat gaaactaagc tgatttctcc acagttcgtt
      841 cgcccgtttg tcaaaagtaa taagaatgat tttgttgat
//
LOCUS       DAJVTY010000336          829 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVTY010000336 DAJVTY010000000
VERSION     DAJVTY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.342"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            1..829
                     /locus_tag="OQQ66_004183"
                     /pseudo
     CDS             1..829
                     /locus_tag="OQQ66_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          233 a          202 c          223 g          171 t
ORIGIN      
        1 cctggttccc gcacggtggc tgaactggaa tctgaaattc tgcaactgcg taaggcgtta
       61 aatgaagctc gccttgagcg agatatatta aaaaaagcaa cagcgtattt tgcacaggag
      121 tcgctgaaaa atacgcgtta atcgaacaat ggcgacaaca atttcccatt gaagcgatgt
      181 gtcaggtatt tggtgtatcc aggagcggtt attacaactg ggtacagcat gaaccctcag
      241 acagaaaaca aagtgatgag cggctaaaac tggagattaa ggtggcacat atccgcactc
      301 gcgaaacata tggaacccgg cggctccaga cggagctggc agagaatggc atcatcgttg
      361 gtcgtgaccg actggcacgt cttcgtaagg agctaaggct acgctgtaag cagaaacgca
      421 agttcagagc gactacgaac ccgaaccaca atctgccagt tgcgccaaat ctgctgaacc
      481 agacgttcgc tcctacagca ccaaatcagg tctgggtggc ggacctgacg tatgttgcca
      541 cacaggaggg atggttgtac ctcgctggca tcaaagatgt ttatacgtgc gaaattgtcg
      601 gctacgccat gggagagcgc atgacaaaag agctgacagg taaagccctg tttatggcgc
      661 tcaggagcca gcgcccacct gccgggctaa tccaccactc tgatcgaggt tcacagtact
      721 gcgcatacga ttaccgggtc atacaggagc agtctggtct gaaaacatca atgtcgcgta
      781 aaggtcactg ttacgacaac gctccgatgg aaagcttctg gggaacgct
//
LOCUS       DAJVTY010000337          819 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVTY010000337 DAJVTY010000000
VERSION     DAJVTY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.343"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>819
                     /locus_tag="OQQ66_004184"
     CDS             <1..>819
                     /locus_tag="OQQ66_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1906861.1"
                     /translation="PSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGAATEDRT
                     QAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPPSLTVLK
                     ASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPDLPPALQ
                     KLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRLPELPQR
                     LQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYHGP"
BASE COUNT          222 a          239 c          137 g          221 t
ORIGIN      
        1 atccttcttt tttccgctcc ttttgtggtt taggatgtat atcccgttta tccgtagaag
       61 agcaaaatat cacggattat caccgcatct gggataactg ggccaaggaa ggtgctgcaa
      121 cagaagaccg aacacaggca gttcgattac tgaaaatatg tctggctttt caagagccag
      181 ccctcaattt aagtttactc agattacgct ctctcccata cctgcccccg cacatacaag
      241 aacttaacat ctctagcaat gagctacgct ctctgccaga actccctccg tccttaactg
      301 tacttaaagc cagcgataac agactgagca ggctcccggc tcttccgcct cacctggtcg
      361 ctcttgatgt ttcacttaac agagttttaa catgtttacc ttctcttcca tcttccttgc
      421 agtcactctc agcccttctc aatagcctgg agacgctacc tgatcttccc ccggctctac
      481 aaaaactttc tgttggcaac aaccagctta ctgccttacc agaattacca tgtgaactac
      541 aggaactaag tgcttttgat aacagattac aagagctacc gccccttcct caaaatctga
      601 ggcttttaaa cgttggggaa aaccaactac acagactgcc cgaacttcca caacgtctgc
      661 aatcactata tatccctaac aatcagctga acacattgcc agacagtatc atgaatctgc
      721 acatttatgc agatgttaat atttataaca atccattgtc gactcgcact ctgcaagccc
      781 tgcaaagatt aacctcttcg ccggactacc acggcccac
//
LOCUS       DAJVTY010000338          817 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVTY010000338 DAJVTY010000000
VERSION     DAJVTY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.344"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            123..>817
                     /locus_tag="OQQ66_004185"
     CDS             123..>817
                     /locus_tag="OQQ66_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1906862.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEHINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          267 a          179 c          132 g          239 t
ORIGIN      
        1 acagttaaat caacatcact tccttaaaat gaaaacaata aatttcaatc ctgaaacatt
       61 ttattttagc ttattatttc ccagcatcac attttatcag gatatccttc tgcgaggtta
      121 ttatgcttcc tgtaaataat ccccccctat ccactggaaa cgtctctttt tacagaacta
      181 catcaatcga caatgttcac aataattatc tctccgaatg ggttgaatgg actaaaaaca
      241 gcatttccgg agaaaacagg gaaactgctt ttacccggct ccaattatgt ctggaaaaca
      301 gtgaaacatc gttggactta tcttgtttag gtctcagatc tctaccacga ttgcctgaca
      361 atcttgatga aattaatgta agcaataacc aactatcaat gctccccgag ctaccaaggg
      421 cattgaaaga actgaatgca agcagtaatc aattatctgc acttcctgaa ttaccagtgt
      481 cgctggaata tataaatgtg agtgataacc atttgttcgc acttcctgaa ttacctgcgt
      541 cactagaaca tattaacgta agtgacaatc acctgtctgt acttccgagg ttaccaatgt
      601 cattggaatt acttgatgca gccagaaatg ctttggaagt aataccagat tttccagaaa
      661 gagatgatca tattataaga atattctggc ttaatcagaa ccggatcacg gcaattccgg
      721 aaagcatact tggcctcagt tctgatagcg ttgtcaatct tagagaaaat caactatctc
      781 ccagaataat gcaaactttg ttacaacaaa ccgccca
//
LOCUS       DAJVTY010000339          809 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVTY010000339 DAJVTY010000000
VERSION     DAJVTY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.345"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..744
                     /locus_tag="OQQ66_004186"
     CDS             <1..744
                     /locus_tag="OQQ66_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCS1906863.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATEWCAEYDEWGNLLNEE
                     NPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     ISEIDPQGLNPLILGVVALMSCGLFSSGGVIQSGQQRRDQYGRKVNPEADKLTDEHKS
                     GVNPGGNCSPQNLSDLQNEKNRLCNQSRACAPQMLRKEILRRYEINLACASVRKQINN
                     QCFGGGDKAHMEEENKAYKTAADCSGLIK"
BASE COUNT          249 a          165 c          208 g          187 t
ORIGIN      
        1 tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct gccgctggcg
       61 cttatcagca cggaaggggc aacagaatgg tgcgcagaat acgatgagtg gggcaacctg
      121 ctgaatgaag agaacccgca ccagctgcag cagcttatcc gtttgccggg gcagcagtat
      181 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcga
      241 tatatcactc aggacccaat tgggctgaag gggggatgga atttttatca gtatccgttg
      301 aatccgatct cagagataga cccacaaggg ttaaatccgt taattcttgg tgttgtagca
      361 ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc aaagtgggca acaacgcaga
      421 gatcaatatg gacgtaaagt taatccagaa gccgacaaat taacagatga acataagtca
      481 ggtgtaaatc ctggaggtaa ctgtagtccc cagaacctga gtgacttaca aaatgaaaaa
      541 aacagattat gtaaccagtc tagagcttgc gctcctcaga tgctgagaaa agaaatattg
      601 agacggtacg agataaattt ggcgtgcgct tcggtcagaa agcagattaa taatcagtgc
      661 tttggtggtg gagataaggc ccatatggaa gaagagaata aagcatataa aacagcagct
      721 gattgcagtg gcttaataaa atgaaaaaaa taattttctc taacgagagt gcagtctatg
      781 atgaacttat gattcatttt ccatgccag
//
LOCUS       DAJVTY010000340          797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVTY010000340 DAJVTY010000000
VERSION     DAJVTY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.346"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..648
                     /gene="ospC4"
                     /locus_tag="OQQ66_004187"
     CDS             <1..648
                     /gene="ospC4"
                     /locus_tag="OQQ66_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1906864.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYK
                     VLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          302 a          108 c          148 g          239 t
ORIGIN      
        1 aaaactgatg ttccaatatt taacactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa ggattcagag agttttgtta taacaaaaat
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagtacaga taacttcaaa aaaataaaac tcagagacat atctttggag
      241 gatgcgatta aagcatctaa ttatgaagag attaacaata aagtgactga taaaaaaatg
      301 gctcatcagg cccttgctta ttctttaggc aataaaaaag ctgatatagc actttattta
      361 ctatctaaat ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaacaacaat
      421 agatattgta atctgtatga tgttgaatac ttacttagta aagatggtgc caactataaa
      481 gtgctagaat attttatcaa taatggattg gttgatgtaa acaaaaaatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aaaatccaaa aatgattgat
      601 tttttattaa aaaatggagc aatattaggc aaacgatttg agatatgacc taaaagctcg
      661 gccaaataat aatgtcgtcg attatcgaag tcatcatgag tgattctgtc cccacagtgc
      721 agcatcagca tgacagtgaa ttgtctggcg tggtccattt aatgagatgg agatggtccc
      781 tctttcctaa atagctg
//
LOCUS       DAJVTY010000341          796 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVTY010000341 DAJVTY010000000
VERSION     DAJVTY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.347"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>796
                     /locus_tag="OQQ66_004188"
     CDS             <1..>796
                     /locus_tag="OQQ66_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1906865.1"
                     /translation="VRPPLEKVMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHH
                     PDKRSARAQRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMG
                     LAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFII
                     DVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKE
                     AGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATL"
     misc_feature    <1..98
                     /locus_tag="OQQ66_004188"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          191 a          190 c          242 g          173 t
ORIGIN      
        1 gagttcgacc gcctctggaa aaagtgatgc cactgctgga taagctgcgt aagctgtacg
       61 gggtcggacc ggtatgcagt gaactacata ttgccccgtc aacgtattac cactgtcagc
      121 aacagcgaca tcatcctgat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga
      181 aagagatact gcgcgtatac gatgggaatc atcaggtata cggtgtgcgt aaagtctggc
      241 gtcagttgtt acgggaaggt atcagagtgg ccagatgcac tgtggcacgt ctcatggcgg
      301 ttatgggact tgccggtgtt ctccggggta aaaaggtccg tacgaccatc agccggaaag
      361 ccgttgccgc aggcgaccgc gtaaaccgtc agttcgtggc agaacgacct gaccagctgt
      421 gggtggctga ttttacttac gtcagcacat ggcagggctt cgtctatgtg gcgttcatta
      481 ttgatgtgtt tgccggatac atcgtggggt ggcgggtctc atcgtccatg gaaacgacat
      541 tcgtgctgga tgctctggag caggcgttat gggcccgtcg accgtccggc acggtccatc
      601 acagtgataa aggttctcag tatgtatcgc tggcctacac acagcggctt aaggaagccg
      661 gattactggc atcaacagga agtacaggcg actcgtatga caacgcgatg gcggagagca
      721 tcaatggtct ttacaaagcg gaggtaatac accgtaagag ctggaaaaac cgtgcagaag
      781 tggaactggc cacact
//
LOCUS       DAJVTY010000342          794 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVTY010000342 DAJVTY010000000
VERSION     DAJVTY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.348"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>794)
                     /locus_tag="OQQ66_004189"
     CDS             complement(<1..>794)
                     /locus_tag="OQQ66_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1906866.1"
                     /translation="SDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTI
                     AVQESSYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQ
                     FAQGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYD
                     VTGNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNCKWVKMLS
                     REETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKE"
BASE COUNT          190 a          211 c          173 g          220 t
ORIGIN      
        1 ctctttacca tcccatgcgc gggatttagc gtaagccgtt tgtgtcgtct ggtcagtttg
       61 gagaatgttg ccatgcaccg tacgccagac agtaaaggtc tctgcctgac catttttcac
      121 cgtaatggtt tcctcacggc ttaacatttt cacccactta caattatgta agtagtagcc
      181 tggtttctct gccgacagcc gttcagcaaa aatatcgaca tcatcgccga aacctgccgt
      241 tgatccccag gaaatcacac cattatgacc aaaaaccagc ccaggatagg caaatggtgt
      301 attgccagtg acatcataac cagcaccgtg cagaccaata ccataagtat acgcaggcgc
      361 ataccagcca aactgcggac catttaccat gattgctttc gcatcctggg ctttgctttt
      421 gccgatcacc cacatattgc tggtcgttgg ataccccgcc agaccattgg caccaccctg
      481 tgcaaattgt gcagcaatag tttcccggtt cttccctgct gttaacgcca gcagtgcgcc
      541 atccgcccct tttgctggtc ggtcaagcat tggtgcaggt aaatcgtagc gtggcaacag
      601 agctgctgtt tgcgagtttt gctgattaaa tttaagtggg tagctactct cttgtacggc
      661 aatagtggtt ggcgctgatg ggtttaccag ccatttcaac tgattaaata ccgccatgcc
      721 ttgtgataca ccatatttat cttttaaagc cgttagcagt gccagattat caatttcgct
      781 agtgctatca gaga
//
LOCUS       DAJVTY010000343          785 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVTY010000343 DAJVTY010000000
VERSION     DAJVTY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.349"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..414)
                     /gene="pdeC"
                     /locus_tag="OQQ66_004190"
     CDS             complement(<1..414)
                     /gene="pdeC"
                     /locus_tag="OQQ66_004190"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1906867.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTP"
BASE COUNT          248 a          162 c          165 g          210 t
ORIGIN      
        1 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
       61 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
      121 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
      181 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
      241 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
      301 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
      361 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
      421 caatttcctg cctgtttata cccggccatt gtaaggtgaa aaccctgggt accgacgtct
      481 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
      541 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
      601 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
      661 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatatttc acaaaattaa
      721 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
      781 cacgc
//
LOCUS       DAJVTY010000344          778 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVTY010000344 DAJVTY010000000
VERSION     DAJVTY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.350"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(32..736)
                     /locus_tag="OQQ66_004191"
     CDS             complement(32..736)
                     /locus_tag="OQQ66_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCS1906868.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          192 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAJVTY010000345          764 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVTY010000345 DAJVTY010000000
VERSION     DAJVTY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.351"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OQQ66_004192"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OQQ66_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1906869.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
BASE COUNT          279 a           98 c          151 g          236 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa gaaatggagc ggtatcaggt aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacg
//
LOCUS       DAJVTY010000346          758 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVTY010000346 DAJVTY010000000
VERSION     DAJVTY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.352"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            222..551
                     /locus_tag="OQQ66_004193"
                     /pseudo
     CDS             222..551
                     /locus_tag="OQQ66_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            581..664
                     /locus_tag="OQQ66_004194"
                     /pseudo
     CDS             581..664
                     /locus_tag="OQQ66_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            664..>758
                     /locus_tag="OQQ66_004195"
     CDS             664..>758
                     /locus_tag="OQQ66_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1906870.1"
                     /translation="MEQKWTSKALSDLARLYDFLVLASKPAAARMV"
BASE COUNT          211 a          168 c          180 g          199 t
ORIGIN      
        1 tcaaaactat caaagcgcag aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga
       61 agcttcacga catagcgggg aagtttgctt ctttgagagg cgggtttacg tttacggggt
      121 ttagctgaac gggccatata accacctgaa agacaatgac attgcctgtt tttataacgg
      181 taattgcaga ccatgacaag ccgcagccgt caggctgcct actcgagctt cagctcaata
      241 aaggcgaata tcgacataaa ttaccccgat ggatcttttt tgcaaatcag ggtgaattca
      301 cgaccggtga ttacgaagaa atcatgaata aggccagcgc cctgagtctg gtatcgaatg
      361 caatcctgta ctggaataca tcaagaataa gcaatattgt tgaagggtta cgtgaaaatg
      421 gtgaggttat tgatgatgac gtattagccc atatatcatt actgccctat aaacacgtat
      481 tacctaatgg tacttatttt gttgatggag aaccttacgg tactgagcct accgaaagag
      541 caaaaccgta agtttctgtt caaatgctcc cgctggtcca tccggacaga ttatcgacca
      601 tcaggctgta caggcctggg cggacagcct cagtactgac aatccgttac cggtgccacg
      661 ctgatggaac agaagtggac cagtaaggcg ctttctgatt tggcgcggtt atatgatttt
      721 ctggtgctgg ccagtaaacc tgctgccgcc agaatggt
//
LOCUS       DAJVTY010000347          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVTY010000347 DAJVTY010000000
VERSION     DAJVTY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.353"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            35..726
                     /locus_tag="OQQ66_004196"
                     /pseudo
     CDS             35..726
                     /locus_tag="OQQ66_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          170 a          181 c          200 g          175 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc cacactggag
      481 cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg
      541 ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
      601 attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg
      661 tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJVTY010000348          675 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVTY010000348 DAJVTY010000000
VERSION     DAJVTY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.354"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            193..462
                     /locus_tag="OQQ66_004197"
     CDS             193..462
                     /locus_tag="OQQ66_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906871.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..675
                     /locus_tag="OQQ66_004198"
                     /pseudo
     CDS             459..675
                     /locus_tag="OQQ66_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          170 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaa
//
LOCUS       DAJVTY010000349          631 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVTY010000349 DAJVTY010000000
VERSION     DAJVTY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.355"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            359..>631
                     /locus_tag="OQQ66_004199"
     CDS             359..>631
                     /locus_tag="OQQ66_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCS1906872.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPL"
BASE COUNT          165 a          137 c          170 g          159 t
ORIGIN      
        1 atgtactgcg gcgcgtgggt cgaaatgagc cagccgttgt tgccaatagg tacgaatacg
       61 ccaattttca tcatcaacct ctcttcgtct cgtaaagtga aagtcagacg gggcgctgca
      121 tcctgcatat ccttttcagc cgcgtattgg cttgtttgca aagcggatgc cagtttttaa
      181 aaagtgaatg ttattaatgt gttagcattg tgttatctaa aatatctggt aaaaagtgga
      241 ctaaacggtc aaaacagttg cacataaaac atgcatccgt gcgcgatgag agtgcagaag
      301 gtcgaggccg ggcgggggtt ttgctatcct gttgccaatc tacaagaggg gagagcgcat
      361 gacgcaaggc gcagtgaaaa caacgggtaa acgttcgcgc gcagtaagcg cgaagaaaaa
      421 agcgattctt agcgcagcac tggacacttt ttcacaattc ggttttcacg gcacaaggct
      481 ggagcagatc gcagagttgg cgggtgtttc aaaaaccaat ctgctgtatt actttccgtc
      541 aaaagaggcg ctgtatattg ccgtgctgcg gcagattctc gatatctggc tggcaccgtt
      601 aaaagcgttt cgtgaagatt tcgccccgct g
//
LOCUS       DAJVTY010000350          631 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVTY010000350 DAJVTY010000000
VERSION     DAJVTY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.356"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..429
                     /locus_tag="OQQ66_004200"
     CDS             <1..429
                     /locus_tag="OQQ66_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1906873.1"
                     /translation="FTLSSANNSTKNAAFYLWGNPSRSVVAELGDDSGWHFFSQRNTD
                     NSITFAVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFRAPSGH
                     GLSGIAISDTGRDSADNVNGVYYRPLQKLINGTWYNVASV"
     gene            432..>631
                     /locus_tag="OQQ66_004201"
     CDS             432..>631
                     /locus_tag="OQQ66_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1906874.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVT"
BASE COUNT          183 a          136 c          162 g          150 t
ORIGIN      
        1 ttcacgttat ccagcgccaa caactccacg aaaaatgcag cgttttattt gtggggtaat
       61 ccatccaggt ctgttgttgc agagcttggc gatgattcag gctggcattt tttcagccag
      121 aggaatacag ataacagcat cacgttcgcc gtaaacggac aggtaattcc gttaaattac
      181 ggaaacttcg atgcccgcta taaatatcga acagaggggg tacaggatgt acggtatggc
      241 catgaaatgt attacagccc cggcagtaac accgtttcgt ggagatttcg cgcaccttcg
      301 ggacacgggc tgtcagggat agcgatatcg gataccggcc gtgactcagc ggataacgtc
      361 aacggcgtgt attaccgccc gcttcagaaa ctgattaatg gcacctggta taacgtggcg
      421 agtgtttaac aatgttgcat ttaaaaaata ttactgcggg caatccgaaa accgtggaac
      481 aatatcagct gacaaaacaa tatgatgtca cctggctttt ttcgaaagac ggaaaaaact
      541 ggtatgaaga gctgaagaat ttcgccagcg acacaattaa aataacttac accggagacg
      601 gtcgcgtggt gtgggtcggt aaggatgtga c
//
LOCUS       DAJVTY010000351          627 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVTY010000351 DAJVTY010000000
VERSION     DAJVTY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.357"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            263..598
                     /gene="iraM"
                     /locus_tag="OQQ66_004202"
     CDS             263..598
                     /gene="iraM"
                     /locus_tag="OQQ66_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1906875.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          253 a          123 c           80 g          171 t
ORIGIN      
        1 atggttcttt ccaccatctg aaaaataatt ctttaaaata cctgacatgt aatacagaaa
       61 aaacacaaaa ccatacctta aataaaaacc tgattatcaa gcagatatgc atggataaac
      121 tacaagacga gatataaacc accctgcatt taaataaaca ataaacaaca tcagaaaaat
      181 aattctgctc tatggtttac attcaaaaat atcatttata cttttcagaa catcaccagc
      241 aaggcataaa caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc
      301 atgcggaata tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc
      361 ggatgctttc ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa
      421 taacaaagtg ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa
      481 tttaatcaaa aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt
      541 taataaccgt tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg
      601 agtttactca catctgacaa aatcaat
//
LOCUS       DAJVTY010000352          618 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVTY010000352 DAJVTY010000000
VERSION     DAJVTY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.358"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..442)
                     /locus_tag="OQQ66_004203"
     CDS             complement(<1..442)
                     /locus_tag="OQQ66_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCS1906876.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKG"
BASE COUNT          152 a          156 c          137 g          173 t
ORIGIN      
        1 tccctttatt gccgccatct gctgtcgaat acggcatgat aactttccct gtcgttggcc
       61 ataaccaaca acgttgccct actggcggcc aggaagattt cggtaccgct gatgacggtg
      121 taaccgatgc ggttttggtc gttgatttgg cggttgattt acgtgtacta ctgctacttt
      181 tcgccccacc cagttttagt ttctgaccaa cttcaatggt gtaagggggg gaaatgccgt
      241 tcagtcgcgc cagttctttt acgctggttc ccgtggtgcg cgaaatacga tatagcgtat
      301 ccccccgttt cacggtgtaa acggagccgg aatatgttcc tgtatcggat gatttgctac
      361 ccgaacagcc cgccaaaagc agtccaaccg ataacaacat cacgataccc agagattttt
      421 tattcaggcg tcccgcactc aaaatctttc ctcgcttaaa aaacaagacg ccttatcata
      481 gcagccagcc tcttgatacc caatctcttg cttctggaaa aacgtggtaa tagagaaaga
      541 ggttaatttc atttggcaaa gggaagtatc atcagcaaaa tattcgctgt agaggtatcg
      601 aacttcctga ctttttac
//
LOCUS       DAJVTY010000353          601 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVTY010000353 DAJVTY010000000
VERSION     DAJVTY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.359"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            18..>601
                     /locus_tag="OQQ66_004204"
     CDS             18..>601
                     /locus_tag="OQQ66_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1906877.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGS"
BASE COUNT          151 a          151 c          164 g          135 t
ORIGIN      
        1 agaacaggga gtgagttatg tccaaaaatg accgaatggt gggcatcagc cgcagaacac
       61 tcgttaaatc aaccgcgata ggttctctgg cgctggctgc aggcggtttt tctttaccgt
      121 tcaccctgcg cagtgcagcg gcaacggtac aacaggccag cgaaaaagtg atctggggtg
      181 cctgttccgt caactgtggt agccgctgtg cacttcgtct acatgttaaa gataatgaag
      241 tgacctgggt ggaaactgac aataccggca gcgatgagta cggcaaccat caggtacgcg
      301 cctgtttgcg cggtcgctcc atccgccggc gtattaatca tcccgatcgc ttgaattacc
      361 caatgaaacg cgtgggtaaa cgcggtgaag gcaaattcga acggattagc tgggatgaag
      421 ccctggatac tatcgccagt agcctgaaaa aaaccgtcga acaatatggc aatgaggctg
      481 tatatattca gtactcttcg gggattgttg gcggcaatat gacccgctct tcgccatcag
      541 cctcggcggt caaacgcctg atgaactgct acggcggttc actcaatcag tatggctcct
      601 a
//
LOCUS       DAJVTY010000354          588 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVTY010000354 DAJVTY010000000
VERSION     DAJVTY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.360"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(41..>588)
                     /locus_tag="OQQ66_004205"
     CDS             complement(41..>588)
                     /locus_tag="OQQ66_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HCS1906878.1"
                     /translation="AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVS
                     IIAQNGRKRQGFRLLSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDS
                     ETARLLHQRLCGWIDPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVAL
                     GWTVTEFAAGKYDITRPKAAG"
BASE COUNT          101 a          203 c          172 g          112 t
ORIGIN      
        1 aaaagataaa aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc
       61 gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct
      121 ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg
      181 acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga
      241 tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct
      301 cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt
      361 tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa
      421 acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc
      481 agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca
      541 gcgcccgata gctacctttg accaccatgg catcagcggt gacggcct
//
LOCUS       DAJVTY010000355          588 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVTY010000355 DAJVTY010000000
VERSION     DAJVTY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.361"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..308)
                     /locus_tag="OQQ66_004206"
     CDS             complement(<1..308)
                     /locus_tag="OQQ66_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1906879.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     "
BASE COUNT          207 a          115 c          103 g          163 t
ORIGIN      
        1 ctggtaatta aaaaaatatg tgccttgaga ttaaactcgt ttaataaagg atatacctga
       61 aaccagttgt caaggtatcc atcatcaaat gtcaacataa cgcttttacg gggaagtttt
      121 ccgccccgat aaaaaaactc cagttcatct gatgacagtg ttttccagtt gttttcagcc
      181 aaccatttca tttgtttccg gaatgtctcg ggagaaagtg taaccagtcc tgggcaatgg
      241 ctgacatgat gatacattaa taccggaaca tgatttgctt taaacaaaat gccccctccg
      301 tttaaaattt aaagtttctg gcacccagta agtagagata aaaggaaagc ctaataaagt
      361 tggtgaactt tatatactac taaatcaaac atcaacaatt tataaacaat aatatattat
      421 catatctttt aaaaacggct cactaagaac cttgaggtgg cacactaaat ttagctacct
      481 gaacagaggt gatatgctca cctcagaaca acacaggtgc cataatgaaa aaagaaattt
      541 cagcgcagag tttaaacgcg aatccgctca actggttgtt gaccagaa
//
LOCUS       DAJVTY010000356          573 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVTY010000356 DAJVTY010000000
VERSION     DAJVTY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.362"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>573
                     /gene="pocR"
                     /locus_tag="OQQ66_004207"
     CDS             <1..>573
                     /gene="pocR"
                     /locus_tag="OQQ66_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCS1906880.1"
                     /translation="LNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPFCQMM
                     RQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVLCGQV
                     RLRNDYVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENCLKKQ
                     LNFVVIKENKSQSDSVRQTRAPSPHDNKMK"
BASE COUNT          147 a          123 c          132 g          171 t
ORIGIN      
        1 cacttaattc agaactcatt aataaaatcg cgcaggattt tgcgcaggcg accagccttg
       61 cggttgtcgt ggtcaatatt cacggcgatg aaatttccga gctgtttaac ttcactccat
      121 tttgtcagat gatgcggcaa caccctcagc taagcactcg atgtcgcatg agcgatcgtt
      181 gtgggggcct ggaagcctct aaatccgatc agccttgtat ttatcgctgt catgccggat
      241 taaccgattt ctctattcca ctggtaatcg ccgggcattt agtcggtttt gtattgtgcg
      301 ggcaagttcg tctgcgtaat gattatgtcg atttggtcga tattctgaat gtcgatgatt
      361 gctggcaggc agatcccgaa ttacttaatg aatttcgcaa agttcctgag atggattact
      421 caagagttat ggcctctgct gatttgttga agttgattgt agaaaattgc ctcaaaaaac
      481 aactcaattt tgtggttatt aaagagaaca aatctcagtc agattcggtg cgccagactc
      541 gtgcgcctag tccccacgac aataaaatga aaa
//
LOCUS       DAJVTY010000357          550 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVTY010000357 DAJVTY010000000
VERSION     DAJVTY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.363"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(47..>550)
                     /locus_tag="OQQ66_004208"
     CDS             complement(47..>550)
                     /locus_tag="OQQ66_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1906881.1"
                     /translation="GRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKF
                     EHRGNSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKM
                     IPGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTV
                     EQLLSLK"
BASE COUNT          148 a          120 c          134 g          148 t
ORIGIN      
        1 tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat tttaaggaaa
       61 gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc cagccctgtt
      121 tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca ggaacggttt
      181 cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga ccatcacccg
      241 gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc atgcggtcga
      301 actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag cccatcacca
      361 ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg cggtgttcga
      421 atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag gattctattt
      481 tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata gaagtagtga
      541 cggcgcgacc
//
LOCUS       DAJVTY010000358          548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVTY010000358 DAJVTY010000000
VERSION     DAJVTY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.364"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>548
                     /locus_tag="OQQ66_004209"
     CDS             <1..>548
                     /locus_tag="OQQ66_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906882.1"
                     /translation="LHAGEAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLC
                     GFDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLG
                     EKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQR
                     DEVALEVEQRVLAHPLYPVLTS"
BASE COUNT          119 a          181 c          140 g          108 t
ORIGIN      
        1 ttacacgccg gtgaagctaa aactgatgct cgtgacgctg ccatcattgc cgaagctgcc
       61 cgtaccctgc ctcacgcgct acgcacgctg aaactggctg acgagcaaat cgccgaactc
      121 tccatgctct gcggcttcga tgatgatctt gccgcacaga caacgcaggc cagcaaccgt
      181 atccgcggcc ttctgaccca gatacatccg gcactggagc gcgttctcgg tccgagactt
      241 gagcacccgg cggtactcga tcttctccag cgatatccct caccagaaaa actcgcttcg
      301 ctgggtgaga agaagctggc agcccagctc tgcaaacttg cgcctcgtct gggtaaacgc
      361 cttgcagcag acatagctca ggcactggcc gaacaaaccg tcgtcgttcc cggcacgaat
      421 gccgctgccg tagtactgcc acgtctggca ctccagctca tcacgctgcg taagcaaaga
      481 gacgaggtgg cgcttgaggt agaacagcga gttcttgctc accctcttta cccggtcctg
      541 accagtat
//
LOCUS       DAJVTY010000359          545 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVTY010000359 DAJVTY010000000
VERSION     DAJVTY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.365"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..464
                     /gene="virK"
                     /locus_tag="OQQ66_004210"
     CDS             <1..464
                     /gene="virK"
                     /locus_tag="OQQ66_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1906883.1"
                     /translation="LTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEA
                     FCALMKACNISECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRL
                     PTQVIRKPLSDIASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
BASE COUNT          164 a          103 c          120 g          158 t
ORIGIN      
        1 ttctgactca aaatggacac attgctttta ttggcggatt acagggagcc cctaaaaata
       61 ccggacctga tgttattcgg tgcgcaaccc gggcctgtta tgggattttc ccaaaacgga
      121 ttatttttga agcattttgt gcgttgatga aagcttgcaa tatctctgag tgtctggcag
      181 tcagtgagca cagtcatgtt ttccggcaat tgagatactg gtatcagaaa cgcaaaacct
      241 ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg aaaaacctgt ggtgactggt
      301 acagattacc aacccaggtg attcgaaagc cgctaagcga tatagccagt aaaaaacgct
      361 ctgagtaccg aaaacgatac gctttgctgg attatatcca tgaaactact atccgctctt
      421 tggatgcata tcctgtacac tcagaacatc aggatttaaa ttaaggtttg atgtattctc
      481 tgaataaaac agtaatgata attttggtag aggcattcgc actaaataat gaaaaaatat
      541 aaatc
//
LOCUS       DAJVTY010000360          542 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVTY010000360 DAJVTY010000000
VERSION     DAJVTY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.366"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            238..>542
                     /locus_tag="OQQ66_004211"
     CDS             238..>542
                     /locus_tag="OQQ66_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1906884.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRA"
BASE COUNT          158 a          105 c          137 g          142 t
ORIGIN      
        1 tggatttctt atgccaaaaa tgatcgtcga tttctccata aaatgtgagc gatgccgaaa
       61 gaaataaaat tagttattgc atttgacgtt tgggtgaaag attatcattt gtcatacaaa
      121 tgaggggtag tatgttgcta gccaattaaa aaagaacgcc atatttattg atgattgatc
      181 ccccggtggg ggggaaacat cgggcataaa tgggcatgaa gtaatggagt attagttgtg
      241 aatgtcaatt tctttgtcac ctgtattggt gacgccctga aatcaagaat ggcacgagac
      301 tccgtgctgc tactggaaaa actcggctgt cgcgtaaatt tcccggagaa acagggatgc
      361 tgcggtcagc ctgcgatcaa tagcggttat atcaaagaag cgattccagg gatgaaaaat
      421 ctgatcgccg cactggagga taacgacgat cccattattt caccggctgg ctcttgcacc
      481 tatgccgtaa aaagttaccc gacgtatctg gcggatgaac ctgaatgggc atcacgtgcc
      541 ga
//
LOCUS       DAJVTY010000361          525 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVTY010000361 DAJVTY010000000
VERSION     DAJVTY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.367"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>525
                     /locus_tag="OQQ66_004212"
     CDS             <1..>525
                     /locus_tag="OQQ66_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCS1906885.1"
                     /translation="LANAKYTAQDATTAQKGIVQLSSATNSTSETLAATSKAVKAVMD
                     ETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNEL
                     AAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKV
                     GRDILAKSTVAAVIE"
BASE COUNT          140 a          149 c          143 g           93 t
ORIGIN      
        1 cttgctaacg caaaatatac cgctcaggac gccacgacgg cacaaaaagg gatagtccag
       61 ctcagtagtg ccaccaacag cacgtctgaa acactggccg cgacatcgaa agcggttaag
      121 gcggtaatgg atgaaacgaa caagaaagcg cccttaaaca gtcctgcgct gaccggaacg
      181 ccaacaacgc cagctgcgcg acagggaacg aataataccc aaatcgcaag cacggctttc
      241 gttatggctg cgattgccgc ccttgtagat tcgtcacctg acgcactgaa tacgctgaac
      301 gagttagcgg cggcgctggg aaacgccccg aattttgcga ccaccatgac taacgcgctt
      361 gcgggtaagc aaccgaaaga tgccaccctg acggcgctgg ccgggcttgc tactgcggca
      421 gacaggtttc cgtattttac ggggaatgat gtcgccagcc tggcaaccct gacaaaagtc
      481 gggcgggata ttcttgcgaa atcgaccgtt gctgccgtta tcgaa
//
LOCUS       DAJVTY010000362          502 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVTY010000362 DAJVTY010000000
VERSION     DAJVTY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.368"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..277
                     /locus_tag="OQQ66_004213"
     CDS             <1..277
                     /locus_tag="OQQ66_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1906886.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAA
                     IIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          122 a          114 c          134 g          132 t
ORIGIN      
        1 cctgaacgtg tactgtcgta atggccaggt ggaaatcgac aacaacatcg gtgaaaacgc
       61 gctgcgatcg gtggcggttg gaagaaaaaa ttatctcttc ttcggttctg acaacggtgg
      121 agaaagcgcg gcgattatct acagtctgct ggtcacctgc aaactgaacg gagtggagcc
      181 ggaggactgg ttgcgcgaag tgatcgtgaa gctcaatgac tggccgtcga accgagtgca
      241 tgaactgctg ccctggaact tctcagctgt aaaataatcc ttccgctacg tacttctctg
      301 ggcgcttacc actcaacggc atcgtcaaaa ttatcaaagc gcagaacata gatttatcat
      361 gtgttttatg gatggctttc tccatcaggc atcgttcgtc acgggaaatt ggtgctatga
      421 tcggcattga tcagtccggt tggtgatttg ttttgatttg gcgattgatc agatcgcaca
      481 atccgggctg agttccctct aa
//
LOCUS       DAJVTY010000363          500 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVTY010000363 DAJVTY010000000
VERSION     DAJVTY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.369"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>500
                     /locus_tag="OQQ66_004214"
     CDS             <1..>500
                     /locus_tag="OQQ66_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1906887.1"
                     /translation="GRLAGDNVPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRP
                     SNINAIAHKLAAAGVTDANYYPEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLP
                     MSVGSTGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRV
                     AHGAVL"
BASE COUNT          119 a          120 c          161 g          100 t
ORIGIN      
        1 gggcgactgg ctggcgataa tgtgcctccc gttgtgctgg ctgatgatca actggatgaa
       61 ctggcagacc tgagaatcat cgacgagggg cgctattgtg tccggctgta caaggcaggc
      121 cacatcaggc catcaaatat taatgccatt gcgcacaagc tggcggcggc gggtgtaact
      181 gatgcgaatt attaccccga agggatgcac agccatatgc gggagaactg gcgcgaatac
      241 ctggaacggg tgcgcgggaa agagccggtg gaagaaaaaa accaccagcg aaaaaccacg
      301 ctaccgatga gcgttggatc taccggatac gacacgcaac tggattacgt ggttaagggg
      361 attattccgg cggtatcgct atgcagcata tacggggcta gcgggtccta taaatcattc
      421 cttgccggat cgtgggcgtg ccatgttgcc actggtcgcc agtggggagg ccgcagggtt
      481 gcacatggtg cggttctcta
//
LOCUS       DAJVTY010000364          491 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVTY010000364 DAJVTY010000000
VERSION     DAJVTY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.370"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>491
                     /locus_tag="OQQ66_004215"
     CDS             <1..>491
                     /locus_tag="OQQ66_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1906888.1"
                     /translation="EYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKLTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPD"
BASE COUNT          168 a           84 c          109 g          130 t
ORIGIN      
        1 gaatattctc atcgcgattg cgaacatcaa atagcaaccg ggaatgctga taatgttatg
       61 cttcgctggt ctgaagtgaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat
      121 acacatactc atacacgttg ggataaaaag ctcacttcac gagaagaaca gtgtaaacat
      181 cttcggcagg acttattgag cgggagagaa tatttaaaaa aaatgacagg aaaatgcagt
      241 aaacatttgt gttggccgga aggttattat aacaaggatt atattcagat cgctgaagaa
      301 cttggattcc attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact
      361 acacggatag gaagaataag caccaaggaa cgggaaaatt gcgtatggct gaaaagacga
      421 cttttttatt atacaacacc gtttttttca tcgttgctgg cccttcataa ggggccaaga
      481 ttaccggatg a
//
LOCUS       DAJVTY010000365          488 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVTY010000365 DAJVTY010000000
VERSION     DAJVTY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.371"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>488
                     /locus_tag="OQQ66_004216"
     CDS             <1..>488
                     /locus_tag="OQQ66_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1906889.1"
                     /translation="FIIIIIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPA
                     TLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVR
                     NPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSL
                     LL"
BASE COUNT          114 a          101 c          102 g          171 t
ORIGIN      
        1 ttttattatc attatcatta tcattctctg ttggggcggt tacagggtct ttactccccg
       61 gcgtaacaac gtttcacatg gtgatacccg tttaatttcc cagcgtatat tctggcagat
      121 tgtgtttcct gcaacgctgt ttctgatact tttccagttt gctgcatttg taggattatt
      181 ggcgagcaga gaaaatctgg tcggtgtcat gccttttaac ctcgggactt taatcaatta
      241 tcaggcctta ctggtgggta atctgatcgg tgtcccgctg tgctacttca tcattcgggt
      301 agtgcgaaat ccattttatt tacgtagcta ttattcgcaa ttaaaacagc aggttgatgc
      361 caaagtcacc aaaaaagagt tcgcgctctg gctactggca ttaggtgctt tattgttgct
      421 gttatgcatg ccgttaaatg aaaaaagcac aatttttagc accaattata ccttgtcatt
      481 attgctgc
//
LOCUS       DAJVTY010000366          473 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVTY010000366 DAJVTY010000000
VERSION     DAJVTY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.372"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>473)
                     /gene="rpnB"
                     /locus_tag="OQQ66_004217"
     CDS             complement(<1..>473)
                     /gene="rpnB"
                     /locus_tag="OQQ66_004217"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1906890.1"
                     /translation="AGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSS
                     AFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLK
                     ALFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALR"
BASE COUNT          123 a          119 c          100 g          131 t
ORIGIN      
        1 atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa tctgtcagca
       61 atggtcatca gtttctcctt ttcttgtggt gcgcgttccg ctatctcacc aataaatgca
      121 cgaaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt tagctgtctg
      181 tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc caacagatcg
      241 cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg ttgcataatc
      301 tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata tattttgcgg
      361 gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata aggacttctg
      421 caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc cgc
//
LOCUS       DAJVTY010000367          442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVTY010000367 DAJVTY010000000
VERSION     DAJVTY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.373"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..353
                     /locus_tag="OQQ66_004218"
     CDS             <1..353
                     /locus_tag="OQQ66_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCS1906891.1"
                     /translation="KMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPVEL
                     ERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKLEK
                     DGVIRIDGDDVAPL"
BASE COUNT          126 a           98 c          132 g           86 t
ORIGIN      
        1 ccaaaatgaa ggacgcggag gaactcaaag aagccgcata tgacttacgc gtggtggagc
       61 tttttaccga cgctgacggt gaattaatca cgtcgctggt ggtggtggat gatccgcgcc
      121 ctcctgttga actggagcgc atcgaggagg cagggaacaa gacggaaaac cataccgcgc
      181 tatgggggtg catccgttca cgcacacaga acggcgacaa gtgcacgatc ccgctgttac
      241 gtgatgacat gaaaaagctg gggtatgaaa tgaaaaactt ccggcgctgg ctgtacaagc
      301 tggaaaaaga tggggttatt cgtatcgatg gggatgatgt agcgccgcta taaaagtgag
      361 gagcaaaagc gagggggata gaaagagggc caaaattagc ccgctctccc tcacttttcg
      421 acctgtatac atcctcaaaa gt
//
LOCUS       DAJVTY010000368          438 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVTY010000368 DAJVTY010000000
VERSION     DAJVTY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.374"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(86..>438)
                     /locus_tag="OQQ66_004219"
     CDS             complement(86..>438)
                     /locus_tag="OQQ66_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906892.1"
                     /translation="TALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTLDV
                     GTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDCWQ
                     ILFFSPDHFNNDFY"
BASE COUNT          146 a           70 c           78 g          144 t
ORIGIN      
        1 cattcatttt tcgacggact aaatcactac taatatcatt accaattgat gaaacaggga
       61 acattatttt atcctttata aataatcaat aaaaatcatt attaaaatga tcgggtgaaa
      121 aaaagagaat ttgccaacaa tctgaagtaa tgcaggtttt attaaagtgc gtaggggctg
      181 ttaagatggc ttctttttgg gtgaactttg cgcaatactt agaaatcgtc tccttaatac
      241 attccagctc ttttttattg acggctgagc aagtacattg ttcaccaaaa ggtgcaaaag
      301 tgcctacatc taatgtttgt atgatataat tatcatgttc tcctgtagaa atatctatga
      361 tgaatggact catattttcc ggttggattc ggatcatgac agaaggaaca tcgccttgat
      421 agaaattcaa tgcagtaa
//
LOCUS       DAJVTY010000369          428 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVTY010000369 DAJVTY010000000
VERSION     DAJVTY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.375"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..394
                     /locus_tag="OQQ66_004220"
     CDS             <1..394
                     /locus_tag="OQQ66_004220"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="catalyzes the ATP-dependent breakage of
                     single-stranded DNA followed by passage and rejoining,
                     maintains net negative superhelicity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase I subunit omega"
                     /protein_id="HCS1906893.1"
                     /translation="CKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGV
                     FLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQ
                     QYVSSEKDGKATGWSAFYVDGKWVEGKK"
BASE COUNT          119 a           96 c          113 g          100 t
ORIGIN      
        1 gtgtaaaaac acacgtaaga ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc
       61 ggtgccatta cctgagctgc cgtgcgaaaa atcagatgct tatttcgtgc tgcgtgacgg
      121 cgctgcgggt gttttcctgg ctgccaacac tttcccgaaa tcgcgtgaaa cgcgtgcgcc
      181 actggtggaa gagctttatc gcttccgcga ccgtctgccg gaaaaactgc gttatctggc
      241 cgatgcgcca cagcaggatc cggaaggtaa taagaccatg gttcgcttta gccgtaaaac
      301 caaacagcaa tatgtctctt cggaaaaaga cggaaaggcg actggctggt cagcatttta
      361 tgttgatggc aaatgggttg aaggaaaaaa ataaccttta attctgtcag gtttttataa
      421 acaaaggg
//
LOCUS       DAJVTY010000370          411 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVTY010000370 DAJVTY010000000
VERSION     DAJVTY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.376"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>411)
                     /locus_tag="OQQ66_004221"
     CDS             complement(<1..>411)
                     /locus_tag="OQQ66_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1906894.1"
                     /translation="WGPAHRRWLSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESAL
                     KEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTRFEHPRQLMSWFGITPSEYS
                     SGGSRHQGSITKAGNSYARKLLVEAAWSYRHPAR"
BASE COUNT           90 a          114 c          100 g          107 t
ORIGIN      
        1 atgcgtgccg gatgacggta actccacgct gcctcgacca atagctttct ggcatagctg
       61 tttccggctt tggtaatgct gccctgatgt ctgcttccac ccgatgaata ttctgatggc
      121 gtgataccaa accatgacat cagttgtcgt ggatgttcaa aacgggtcag atcacccagt
      181 tcagaaatta atccaaccgc agtaataaac tgaatccctc gcatggcttg cagcgcctct
      241 actaccggat acagcctcca ttcggtaacg gcctccttca gggcggattc cagccgttca
      301 cattgtgcct gccggtcttc tattgtacga cggtgttcgt caaaagctaa ttgtcgccag
      361 ggactttcga atgagtattt gctgagccag cgtcggtgtg cagggcccca a
//
LOCUS       DAJVTY010000371          409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVTY010000371 DAJVTY010000000
VERSION     DAJVTY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.377"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            46..409
                     /locus_tag="OQQ66_004222"
                     /pseudo
     CDS             46..409
                     /locus_tag="OQQ66_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          112 a           87 c          103 g          107 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctaatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc gaacccaggt caggacatgg gttgccctct
      181 atgaaaaaca tggagaaaaa ggcttaattc ccaaacctaa aggtgttagt gctgatccag
      241 agttgcgtat taaggtcgtg aaagctgtga ttgagcagca catgtccctt aatcaggctg
      301 ctgctcactt tatgcttgct ggtagtggtt ctgtagccag gtggctgaag gtttatgaag
      361 agcgcggaga agctggttta cgcgcgctca agattggcac caaaagaaa
//
LOCUS       DAJVTY010000372          409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVTY010000372 DAJVTY010000000
VERSION     DAJVTY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.378"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>409
                     /locus_tag="OQQ66_004223"
     CDS             <1..>409
                     /locus_tag="OQQ66_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1906895.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLL"
BASE COUNT           81 a          128 c          130 g           70 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctcca
//
LOCUS       DAJVTY010000373          388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVTY010000373 DAJVTY010000000
VERSION     DAJVTY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.379"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            75..>388
                     /locus_tag="OQQ66_004224"
     CDS             75..>388
                     /locus_tag="OQQ66_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HCS1906896.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EAT"
BASE COUNT          123 a           81 c           91 g           93 t
ORIGIN      
        1 aatataaaac cacattttta gtggtagttt atggcggtga atttttccag acaaatacaa
       61 aaactggagt tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacag
      121 agattgtcgc taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata
      181 aactaaaaca gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga
      241 tgttcgacaa catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg
      301 agattatgtt cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca
      361 tactcaaaca agccgtggaa gaggcaac
//
LOCUS       DAJVTY010000374          387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVTY010000374 DAJVTY010000000
VERSION     DAJVTY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.380"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            117..>387
                     /gene="ycaL"
                     /locus_tag="OQQ66_004225"
     CDS             117..>387
                     /gene="ycaL"
                     /locus_tag="OQQ66_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1906897.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNING"
BASE COUNT          120 a           74 c           87 g          106 t
ORIGIN      
        1 attgtcggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
       61 caatgtaagt tgttgataca taatgtttat atatgattaa tcaacggatg attcacatga
      121 agaatactaa attactgctg gcgattgcga cctctgcagc attactgaca gggtgtcaaa
      181 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
      241 caacattaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
      301 gcggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
      361 aagcattggg taacaatatt aatggca
//
LOCUS       DAJVTY010000375          380 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVTY010000375 DAJVTY010000000
VERSION     DAJVTY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.381"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>380)
                     /locus_tag="OQQ66_004226"
     CDS             complement(<1..>380)
                     /locus_tag="OQQ66_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1906898.1"
                     /translation="AYCIKYAILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIE
                     NLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYH
                     GMLYRMCALVVISGITGGLAYPLL"
BASE COUNT          139 a           81 c           64 g           96 t
ORIGIN      
        1 tcagcagtgg ataagccaga ccgcccgtaa tcccggatat cactaccaac gcacacatgc
       61 gataaagcat accgtggtac gttaaagttg agcgaaaacg aatacgccca tcattcagtg
      121 acaggttact cataaaatga tttcgcaacg acaccgttaa ataactggtg ctaacagcaa
      181 tcccaaaata atagattaac tgcgcgatta tcattttacg ttgcatttcc ataaattgct
      241 gtaaattctc aatatcatca tttgcataca cagaactatc atacgcattt aatatttgtt
      301 cgaagataat ataaccagca acagcgagaa aaggtaataa agctaaaaat gcgaggatgg
      361 catattttat acaatacgca
//
LOCUS       DAJVTY010000376          378 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVTY010000376 DAJVTY010000000
VERSION     DAJVTY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.382"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..277
                     /gene="yafV"
                     /locus_tag="OQQ66_004227"
     CDS             <1..277
                     /gene="yafV"
                     /locus_tag="OQQ66_004227"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1906899.1"
                     /translation="PAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRV
                     INPQGEIIATADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT           78 a           88 c          130 g           82 t
ORIGIN      
        1 gcctgctccg cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca
       61 ggcgtatgtg gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg
      121 tgacagccgg gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc
      181 aacgcgcatt gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc
      241 ggcatggcag gatgcggatg agtttaggtt gcggtgagtg ggggttgtgt ttaaggctgg
      301 gggagagtcg gggtaagact acgaaagccc gctctccgca aggactgacg ccagatagtt
      361 tctgtccata gctgcttt
//
LOCUS       DAJVTY010000377          377 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVTY010000377 DAJVTY010000000
VERSION     DAJVTY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.384"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            179..334
                     /locus_tag="OQQ66_004228"
                     /pseudo
     CDS             179..334
                     /locus_tag="OQQ66_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          118 a           82 c           95 g           82 t
ORIGIN      
        1 cagaacggga tctatacttt tgcgggtcaa cataagagga tgagtcctta ataaaacagg
       61 tcctcagaga atacatattc tgcggagcgt gcggcaacaa caggacgaac aggggcggtt
      121 ttcaatttgc agccgtcagg ctgccgtggt tcactccaac aacggagaca ggtaaattat
      181 ggacgaaaag aagcttaaag cccttgcggc tgaactggct aaaggcctca aaaccgaggc
      241 cgacctcaat cagttttccc gtatgcagac gaaattaact gtcgaaacgg tgcttaatga
      301 ggaactaact gaccatctcg ggcatgaaaa aagctacatc cggtagtaaa cattatcagt
      361 gggttatgcg atatgat
//
LOCUS       DAJVTY010000378          374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVTY010000378 DAJVTY010000000
VERSION     DAJVTY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.385"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..313
                     /locus_tag="OQQ66_004229"
     CDS             <1..313
                     /locus_tag="OQQ66_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCS1906900.1"
                     /translation="QYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVANQRKH
                     ETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDSFCRGV
                     A"
     gene            310..>374
                     /locus_tag="OQQ66_004230"
     CDS             310..>374
                     /locus_tag="OQQ66_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906901.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
BASE COUNT           96 a          111 c           93 g           74 t
ORIGIN      
        1 gcagtacacc cgtaacagtt tttacatccc actaatgact cgcctgcgcc cgatggggat
       61 cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc tggctgcacg atgtcgctaa
      121 ccaacgaaag catgaaacaa tccaggcccg tccctgcgat cgctggctcg aagagcagca
      181 gtccatgctg gcactgcctc cggagaaaaa agagtatgac gtgcatcctg gtgaaaatct
      241 ggtgaacttc gataaacacc ccctgcatca tccactctcc atctacgact cattctgcag
      301 aggagtggcg tgatgatgga actgcaacat caacgactga tggcgctcgc cgggcagttg
      361 caactggaaa gcct
//
LOCUS       DAJVTY010000379          374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVTY010000379 DAJVTY010000000
VERSION     DAJVTY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.386"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..181)
                     /locus_tag="OQQ66_004231"
     CDS             complement(<1..181)
                     /locus_tag="OQQ66_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1906902.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEV"
BASE COUNT          144 a           66 c           50 g          114 t
ORIGIN      
        1 taacttccat attagcatag agataacgct tacatttcat taatgcccat ggtaaataga
       61 tacctagcgt tataatcgtt aacaacacat tcaccacaca aataagaaaa tatctctcac
      121 ctgtaccatg aaaaacaaac gaatgggacg gaacatccat ttcattaata acttgagcca
      181 taaaaattcc ctgaattaaa aaatccattg atataattta tatatacaga cagtaaaatg
      241 ttctgccacg ttacaatact cttatcagta ataggctggc aaaaacttta ttgctttata
      301 agatgcttgc gcacgtaaga agagagtggt attaaacaaa tgtataatta ctttattttt
      361 attgggttaa acta
//
LOCUS       DAJVTY010000380          366 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVTY010000380 DAJVTY010000000
VERSION     DAJVTY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.387"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..251
                     /locus_tag="OQQ66_004232"
     CDS             <1..251
                     /locus_tag="OQQ66_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1906903.1"
                     /translation="RLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          122 a           74 c           78 g           92 t
ORIGIN      
        1 gacgtctgat gaacatcagt caggctatga ttataccact tttcccggtt tatgatgaaa
       61 aaaaacatct cctgactatt gaaattcggc caccaatgga tgcttgcatt gccagcgcgg
      121 acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg gggccacatc
      181 cggaacagta tgtctgggtt ttaaaattgt taaaaacacg caaatcaaac gaagcggacc
      241 cgtacccttg aaattataaa gtataagtat ttccactagt tgtttgtaag cgtacagcct
      301 gaaccgtctg gtcagaatct gacgaattag acaaagtggt gtccaccaaa taagtagtgg
      361 gaacca
//
LOCUS       DAJVTY010000381          363 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVTY010000381 DAJVTY010000000
VERSION     DAJVTY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.388"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>363
                     /locus_tag="OQQ66_004233"
     CDS             <1..>363
                     /locus_tag="OQQ66_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906904.1"
                     /translation="VTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLS
                     DGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLH
                     KVTTTVSKNHAMIVIEDL"
BASE COUNT          114 a           90 c           85 g           74 t
ORIGIN      
        1 atgtcactgt cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag
       61 tatcaactcc ggttcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac
      121 tcgccacgct gtcagatggc acagtctttg agcctgtaaa cagttttcag aaaaaccaga
      181 agaagctggc gagacttcag cgacagttaa gccgcaaggt caaattcagc aacaactggc
      241 agaagcagaa acgcaaaata cagcgactgc attcctgtat cgcaaatatc cgcagggact
      301 accttcacaa agtcacaacg accgtcagca aaaaccacgc aatgatagtc attgaggatt
      361 tga
//
LOCUS       DAJVTY010000382          358 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVTY010000382 DAJVTY010000000
VERSION     DAJVTY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.389"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            13..171
                     /locus_tag="OQQ66_004234"
     CDS             13..171
                     /locus_tag="OQQ66_004234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906905.1"
                     /translation="MNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSN
                     LICRALRK"
BASE COUNT          112 a           69 c           88 g           89 t
ORIGIN      
        1 aatacggaaa aattgaacga tgcagtggaa gagctgcgtc agttgctcaa taaccaccat
       61 gacctgaagg ttgtggaaac accaatctat ggttatgtgt ggctgaacat ggaaacggca
      121 catcagcttg agttgctatc gaatctgatt tgccgggcct tgcgcaaata attcctgaac
      181 tacagaatca tcttgctgct gcttcgattc agcaaggata aagggtatga tagtgaaaag
      241 ggataaaagc attgtcatct gcggcagcta tgagtaatgt tggctctaac ggatagcggt
      301 tgcttaaacg aatccgactc tcacattatc aggggtataa aaatggaaac taccaagc
//
LOCUS       DAJVTY010000383          355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVTY010000383 DAJVTY010000000
VERSION     DAJVTY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.390"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..>355)
                     /gene="capU"
                     /locus_tag="OQQ66_004235"
     CDS             complement(<1..>355)
                     /gene="capU"
                     /locus_tag="OQQ66_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1906906.1"
                     /translation="RLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTWKHPFRIIRQKT
                     YLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDFQELYVDSRNSL
                     PPSVLSWLASRRGCPV"
BASE COUNT          110 a           81 c           68 g           96 t
ORIGIN      
        1 acggggcatc ctcttcggga tgccagccag gaaagaacac tcggaggcaa actgtttcgg
       61 gaatcaacgt ataattcctg gaaatcaaag ccaggaggca caacagtaac ccgagttcga
      121 catccttcct gctccagatg tgtcttcata tttgttccgg gaacaatcac ctcatcgcaa
      181 aaatgattta ttgaaaaaaa ttttgttttt cgtgtcaaat atgtcttttg cctgataatt
      241 ctgaatggat gtttccaggt aaaaaaccgt accaaaccaa caatattact atcatgtcca
      301 ctgtgacaaa cgattgcatc gggctgaaaa ctatgaacta ttccgagtaa tctcc
//
LOCUS       DAJVTY010000384          355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVTY010000384 DAJVTY010000000
VERSION     DAJVTY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.391"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            81..>355
                     /locus_tag="OQQ66_004236"
     CDS             81..>355
                     /locus_tag="OQQ66_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906907.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLT"
BASE COUNT           97 a           97 c           78 g           83 t
ORIGIN      
        1 ttaacagtgg catcgcatcc gcagagatgc tttctcgtga cagtgaaaat ttcaacatat
       61 aagaaaaagt cacctgcaaa atggaaaaca atcgcaattt ccctgccaga caatttcatt
      121 cgctcacgtt ctttgccggt ctttgtattg gcatcacgcc tgtggctcag gcactcgccg
      181 ccgaagggca aactaacgcg gatgacacgc tggttgtcga agcatcaacg ccttcgcttt
      241 atgcgccaca acaatctgcc gatccgaaat tctcgcgtcc ggtagcggat actacccgca
      301 cgatgacggt gatttctgaa caagtgatta aagatcaggg cgcaaccaac cttac
//
LOCUS       DAJVTY010000385          348 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVTY010000385 DAJVTY010000000
VERSION     DAJVTY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.392"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>348
                     /locus_tag="OQQ66_004237"
     CDS             <1..>348
                     /locus_tag="OQQ66_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1906908.1"
                     /translation="WEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKP
                     VRMIILDTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARG
                     SSAFRASLDAEYRI"
BASE COUNT           74 a           75 c          113 g           86 t
ORIGIN      
        1 tgggaggttg tgcacgatga gcaggtgaaa aatctgtatc tggtaaaccg ccccatcttt
       61 ccggctgccc cgcttgatgt tgatgaaatg gttatcgctg cccgtcaggt ggagcgggaa
      121 acgggtaaac ctgtacgcat gattattctg gatacgctgg cgcgttgctt tggtgggaat
      181 gatgaaaatg attcccgtga tatgggggcg tttatccgtg gttgtgacga actgaaacga
      241 cgcacagggg ccacggtgct ggtggttcac cattccggca aggatgagac gaaaggcgcg
      301 cgcggttcca gtgcatttcg tgcttcgctg gatgctgaat accggata
//
LOCUS       DAJVTY010000386          348 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVTY010000386 DAJVTY010000000
VERSION     DAJVTY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.393"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>348
                     /locus_tag="OQQ66_004238"
     CDS             <1..>348
                     /locus_tag="OQQ66_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1906909.1"
                     /translation="RFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDN
                     MRYDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMG
                     FSPRLCRPFRAQT"
BASE COUNT           71 a           92 c           95 g           90 t
ORIGIN      
        1 ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact atgcgtaatg
       61 gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg ttgtacatcg
      121 aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat gcgttccgct
      181 tctttggtgg tgtgccgcgc gaagtgttgt atgacaatat gaaaactgtg gttctgcaac
      241 gtgacgcata tcagaccggt cagcaccggt tccatccttc gctgtggcag ttcggcaagg
      301 agatgggctt ctctccccga ctgtgtcgcc ccttcagggc acagacta
//
LOCUS       DAJVTY010000387          327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVTY010000387 DAJVTY010000000
VERSION     DAJVTY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.394"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..174
                     /locus_tag="OQQ66_004239"
     CDS             <1..174
                     /locus_tag="OQQ66_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1906910.1"
                     /translation="IILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCL
                     HNSCIIKICSICY"
     gene            complement(242..>327)
                     /locus_tag="OQQ66_004240"
     CDS             complement(242..>327)
                     /locus_tag="OQQ66_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HCS1906911.1"
                     /translation="TYDFERLMEGAKLLKCSEFGEAIIENM"
BASE COUNT          121 a           62 c           47 g           97 t
ORIGIN      
        1 attatccttc aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
       61 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
      121 aataaatgtt tacataatag ttgcattata aaaatatgct cgatatgcta ttaataatat
      181 ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac tcatggagag
      241 attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc ttagcgcctt
      301 ccatcagacg ttcaaagtca taagtta
//
LOCUS       DAJVTY010000388          326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVTY010000388 DAJVTY010000000
VERSION     DAJVTY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.395"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>326
                     /locus_tag="OQQ66_004241"
     CDS             <1..>326
                     /locus_tag="OQQ66_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1906912.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJVTY010000389          311 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVTY010000389 DAJVTY010000000
VERSION     DAJVTY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.397"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>311
                     /locus_tag="OQQ66_004242"
     CDS             <1..>311
                     /locus_tag="OQQ66_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1906913.1"
                     /translation="RLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMS
                     LSRKPEVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELAKTQRRSLA
                     D"
BASE COUNT           72 a           90 c          103 g           46 t
ORIGIN      
        1 accggctggt gcactacacg cggacacaat atgcagagcc gctggtcgaa agtcgctatc
       61 tttacgaccc gctgggccgc agggtggcaa aacgggtgtg gcggcgtgaa cgtgacctga
      121 cgggctggat gtcgctgtca cggaaaccgg aagtgacctg gtacggctgg gacggcgacc
      181 ggctgaccac aatacagaac gacaggagcc gcatccagac gatttatcag ccggggagct
      241 tcacgccact catcagagtt gaaaccgcca ccggtgagct ggcgaaaacg cagcgccgca
      301 gcctggcgga t
//
LOCUS       DAJVTY010000390          302 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVTY010000390 DAJVTY010000000
VERSION     DAJVTY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.denovo.398"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..>302
                     /locus_tag="OQQ66_004243"
     CDS             <1..>302
                     /locus_tag="OQQ66_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1906914.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKV"
BASE COUNT           63 a           89 c           79 g           71 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gttccgcctc gaaattctgg aggacattgc ccgggataaa
      301 gt
//
LOCUS       DAJVTY010000391         5979 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.guided.200, whole genome shotgun sequence.
ACCESSION   DAJVTY010000391 DAJVTY010000000
VERSION     DAJVTY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.guided.200"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            310..2118
                     /gene="frdA"
                     /locus_tag="OQQ66_004244"
     CDS             310..2118
                     /gene="frdA"
                     /locus_tag="OQQ66_004244"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1906915.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2111..2845
                     /gene="frdB"
                     /locus_tag="OQQ66_004245"
     CDS             2111..2845
                     /gene="frdB"
                     /locus_tag="OQQ66_004245"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1906916.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2856..3251
                     /gene="frdC"
                     /locus_tag="OQQ66_004246"
     CDS             2856..3251
                     /gene="frdC"
                     /locus_tag="OQQ66_004246"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1906917.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3262..3621
                     /gene="frdD"
                     /locus_tag="OQQ66_004247"
     CDS             3262..3621
                     /gene="frdD"
                     /locus_tag="OQQ66_004247"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1906918.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3684..4817
                     /locus_tag="OQQ66_004248"
     CDS             3684..4817
                     /locus_tag="OQQ66_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1906919.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4906..5439
                     /gene="blc"
                     /locus_tag="OQQ66_004249"
     CDS             4906..5439
                     /gene="blc"
                     /locus_tag="OQQ66_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1906920.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGLFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5436..5753)
                     /gene="sugE"
                     /locus_tag="OQQ66_004250"
     CDS             complement(5436..5753)
                     /gene="sugE"
                     /locus_tag="OQQ66_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1906921.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         1475 a         1557 c         1594 g         1353 t
ORIGIN      
        1 ttttgacaag ggcacgaagt ctactcgcaa cgcgacggcg agacaaattt tacgcaggaa
       61 tcaaacagcg gtgggcagtg actaaaaaaa gcacgatctg atggtttagt aattaaatta
      121 atcatcttca gtgataattt agccctcttg cgcactaaaa aaatcgatct cgtcaaattt
      181 cagacttatc catcagacta tactgttgta cctataaagg agcagtggaa tagcgttcgc
      241 agaccgtaac tttcaggcac ttaccctgaa gtacggggct gtgggataaa aacaatctgg
      301 aggaatgtcg tgcaaacctt tcaagccgat cttgccattg taggcgccgg tggcgcggga
      361 ttacgtgctg caattgctgc cgcgcaggca aatccaaatg caaaaatcgc actaatctca
      421 aaagtatacc cgatgcgtag ccataccgtt gctgcagaag ggggctccgc cgctgtcgcg
      481 caggatcatg acagcttcga atatcacttt cacgatacag tagcgggtgg cgactggttg
      541 tgtgagcagg atgtcgtgga ttatttcgtc caccactgcc caaccgaaat gacccaactg
      601 gaactgtggg ggtgcccatg gagccgtcgc ccggatggta gcgtcaacgt acgtcgcttc
      661 ggcggcatga aaatcgagcg cacctggttc gccgccgata agaccggctt ccatatgctg
      721 cacacgctgt tccagacctc tctgcaattc ccgcagatcc agcgttttga cgaacatttc
      781 gtgctggata ttctggttga tgatggtcat gttcgcggcc tggtagcaat gaacatgatg
      841 gaaggcacgc tggtgcagat ccgtgctaac gcggtcgtta tggctactgg cggtgcgggt
      901 cgcgtttatc gttacaacac caacggcggc atcgttaccg gtgacggtat gggtatggcg
      961 ctaagccacg gcgttccgct gcgtgacatg gaattcgttc agtatcaccc aaccggtctg
     1021 ccaggttccg gtatcctgat gaccgaaggc tgccgtggtg aaggcggtat tctggtcaac
     1081 aaaaatggct accgttatct gcaagattac ggcatgggcc cggaaactcc gctgggcgag
     1141 ccgaaaaaca aatatatgga actgggtcca cgcgacaaag tttctcaggc cttctggcac
     1201 gaatggcgta aaggcaacac catctccacg ccgcgtggcg atgtggttta tctcgacttg
     1261 cgtcacctcg gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga actggcgaaa
     1321 gcgtacgttg gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac cgcacactac
     1381 accatgggcg gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc
     1441 gtgggtgaat gttcctctgt tggtctgcac ggtgcaaacc gtctgggctc caactccctg
     1501 gcggaactgg tggtcttcgg ccgtatggcc ggtgaacaag cgacagagcg tgcagcaact
     1561 gccggtaatg gcaacgaaga ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg
     1621 aaagatctgg ttaaccagga tggcggcgaa aactgggcga agatccgcga cgaaatgggc
     1681 ctagcaatgg aagaaggttg cggtatctac cgtacgccgg aactgatgca gaaaaccatc
     1741 gacaagctgg cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga cacctccagc
     1801 gtgttcaaca ccgacctgct ctacaccatt gaactgggcc acggtctgaa cgttgctgaa
     1861 tgtatggcgc actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac
     1921 gaaggttgca ccgagcgtga cgacgtcaac ttcctcaaac acaccctcgc cttccgcgat
     1981 gctgatggca cgactcgcct ggagtacagc gacgtgaaga ttactacgct gccgccagct
     2041 aaacgcgttt acggtggcga agcggatgca gccgataagg cggaagcagc caataagaag
     2101 gagaaggcga atggctgaga tgaaaaacct gaaaattgag gtggtgcgct ataacccgga
     2161 agtcgatacc gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt
     2221 actggatgcg ctgggctaca tcaaagacaa cctggcaccg gacctgagtt accgctggtc
     2281 ctgccgtatg gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc caaaactggc
     2341 atgtaaaacc ttcctgcgtg attacaccga cggtatgaag gttgaagcgt tagctaactt
     2401 cccgattgaa cgcgatctgg tggtcgatat gacccacttc atcgaaagtc tggaagcgat
     2461 caaaccgtac atcatcggca actcccgcac cgcggatcag ggtactaaca tccagacccc
     2521 ggcgcagatg gcaaagtatc accagttctc cggttgcatc aactgtggtc tgtgctacgc
     2581 cgcgtgcccg cagtttggcc tgaacccaga gttcatcggt ccggctgcca ttacgctggc
     2641 gcatcgttat aacgaagata gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa
     2701 cagccagaac ggcgtatgga gctgtacttt cgtgggctat tgctccgaag tctgcccgaa
     2761 acacgtcgat ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct
     2821 tatcgcgacc ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg
     2881 tacggccaat gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc
     2941 gcgaaggcac ggcggttccg gctgtgtggt tcagcattga actgattttc gggctgtttg
     3001 ccctgaaaaa tggcccggac gcctgggcgg gattcgtcga ctttttacaa aacccggtta
     3061 tcgtgatcat taacctgatc actctggcgg cagctctgct gcacaccaaa acctggtttg
     3121 aactggcacc gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa
     3181 ttatcaaaag tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc
     3241 tgtactggta aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta
     3301 ttctggggcc tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc
     3361 ctgctggtgg gtattctgct gccactgggg ctgtttccgg gtgatgcgct gagctacgag
     3421 cgcgttctgg cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt
     3481 ctgccgctgt ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaattcac
     3541 gtgcctgcgg gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg
     3601 attggtatcg ttacaatcta acgtatcgcc aatgtaaatc cggcccgcct atggcgggcc
     3661 gttttgtatg gaaaccagac cttatgttca aaacgacgct ctgcgcctta ttaattaccg
     3721 cctcttgctc cacatttgct gcccctcaac aaatcaacga tattgtgcat cgcacaatta
     3781 ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta
     3841 aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc
     3901 aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgatg
     3961 ctattgctcg aggggaaatc aagttaagcg atcccgcaac aaaatactgg cctgaactta
     4021 ccgctaaaca gtggaatggg atcacactat tacatcttgc gacctacacc gctggcggcc
     4081 tgccattgca gatgccggat gaagtgaaat cctcaagcga cttgctgcgc ttctatcaaa
     4141 actggcagcc tgcatgggct ccaggaacac aacgtctgta tgccaactcc agtatcggtt
     4201 tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaactc
     4261 gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtaccgccc gcagaagaaa
     4321 agaattacgc ctggggatat cgcgaaggca aggcagttca tgtttcgcca ggggcgttag
     4381 atgctgaagc ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg gtgcaaagca
     4441 atttaacccc ccttgatatc aatgagaaaa cgcttcaaca agggatacaa ctggcacaat
     4501 ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg ctggactggc
     4561 cggtcaatcc tgacagcatc attaacggca gtgacaataa aattgcactg gcagcacgcc
     4621 cggtaaaagc gattaagccc ccaactcctg cagtacgcgc atcatgggta cataaaacgg
     4681 gggcgaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatcg
     4741 tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tgtcagattc
     4801 tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc
     4861 cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct
     4921 ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gcagttctcc tacgccgccg
     4981 cgtggcgtga cagtagtgaa taatttcgac gctaaacgct atctcggtac ctggtatgag
     5041 attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc aacatacagt
     5101 ctgcgtgatg gcggcggcct gaatgtcatc aataaaggct ataaccctga cagagaaatg
     5161 tggcagcaga gtgaagggaa agcgtacttt accggcgcac caacacgcgc tgcgctgaaa
     5221 gtgtcattct ttggtctttt ctatggcggt tataacgtca ttgcacttga tcgggaatac
     5281 cgccatgcgc tggtttgcgg cccggaccgc gactacctgt ggatactctc ccgcacgcca
     5341 accatttctg acgaagtgaa acaggagatg ctggcagtcg cgacccggga agggtttgat
     5401 gtcagtaagt ttatttgggt acagcagccc ggtagttagt gagtgctgag tttcagacca
     5461 ataatcccca atacgattaa cgccagactc gccaggcgca tcgggttagc ggactcaccg
     5521 agcagcacaa tgccggtgat agccgcgccg actgcgccaa tacccgtcca cacggcataa
     5581 gccgtcccta ctggtaacga tttcatcgcc caggcaagta gcgccatact gacaatcatc
     5641 gccgtcacgg taataacact cggcgtcaaa cgactaaagc cgtgggtata tttcaggcca
     5701 acggcccata ccacttccag cagaccagca ataactaaga taatccagga catatcaggc
     5761 tccggagaat tggggccgtc cccggtgaaa agacgcgttt gcaggtcgtc ctgcaaagct
     5821 gatggttgaa cggatatttt gccaggtggc aggggaattt caactattac cttagtgact
     5881 atcgcagaaa aaacaaacgg cacgacacag ctgtcgtacc gttattgctt attgctattg
     5941 ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcc
//
LOCUS       DAJVTY010000392         3071 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.guided.254, whole genome shotgun sequence.
ACCESSION   DAJVTY010000392 DAJVTY010000000
VERSION     DAJVTY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.guided.254"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(176..826)
                     /gene="tetR(A)"
                     /locus_tag="OQQ66_004251"
     CDS             complement(176..826)
                     /gene="tetR(A)"
                     /locus_tag="OQQ66_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1906922.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            932..2131
                     /gene="tet(A)"
                     /locus_tag="OQQ66_004252"
     CDS             932..2131
                     /gene="tet(A)"
                     /locus_tag="OQQ66_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1906923.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2163..3023)
                     /locus_tag="OQQ66_004253"
     CDS             complement(2163..3023)
                     /locus_tag="OQQ66_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCS1906924.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          576 a          958 c          949 g          588 t
ORIGIN      
        1 cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg cttggacgct agggcagggc
       61 atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat
      121 gttgtctaca tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat
      181 cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat
      241 cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt
      301 ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc
      361 cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg
      421 tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct
      481 gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc
      541 catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc
      601 gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg
      661 ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa
      721 cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca
      781 ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat
      841 cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc
      901 ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat
      961 tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg
     1021 cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc
     1081 gctgtatgcg ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt
     1141 cgggcggcgg ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact acgccatcat
     1201 ggcgacggcg cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg
     1261 ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc
     1321 gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct
     1381 cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa
     1441 cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg
     1501 gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac
     1561 cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc
     1621 cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat
     1681 ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt
     1741 agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg
     1801 ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct
     1861 tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga
     1921 ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt
     1981 cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc
     2041 atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg
     2101 gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa cgataggcct atgccatgcg
     2161 ggtcaaggcg acttccggca agctatacgc gccctaggag tgcggttgga acgttggccc
     2221 agccagatac tcccgatcac gagcaggacg ccgatgattt gaagcgcact cagcgtctga
     2281 tccaagaaca accatcctag caacacggcg gtccccgggc tgagaaagcc cagtaaggaa
     2341 acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct
     2401 ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc
     2461 ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg
     2521 gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc
     2581 atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt
     2641 gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat
     2701 cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg
     2761 cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag
     2821 aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga
     2881 atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag catcgcgacc
     2941 gtcatcggtg agaagttcgg caggtattgg gtggtgacaa tgtaggtgct gccccaaatg
     3001 gcaggtgcta tcgctgtgaa caataaatcg gggcagacgg cgcttccagc gtgacgacca
     3061 tgccgagcac g
//
LOCUS       DAJVTY010000393         2875 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.guided.260, whole genome shotgun sequence.
ACCESSION   DAJVTY010000393 DAJVTY010000000
VERSION     DAJVTY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.guided.260"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            313..1128
                     /gene="sul2"
                     /locus_tag="OQQ66_004254"
     CDS             313..1128
                     /gene="sul2"
                     /locus_tag="OQQ66_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1906925.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1189..1992
                     /locus_tag="OQQ66_004255"
                     /note="aph(3'')-Ib"
     CDS             1189..1992
                     /locus_tag="OQQ66_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1906926.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1992..2828
                     /locus_tag="OQQ66_004256"
     CDS             1992..2828
                     /locus_tag="OQQ66_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1906927.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          583 a          788 c          811 g          693 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccat cttttatatt caatggctta
       61 ttttcctgct aattggtaat accatgaaaa ataccatgct cagaaaaggc ttaacaatat
      121 tttgaaaaat tgcctactga gcgctgccgc acagctccat aggccgcttt cctggctttg
      181 cttccagatg tatgctattc tgctcctgca gctaatggat caccgcaaac aggttactcg
      241 cctggggatt ccctttcgac ccgagcatcc gtatgagact catgctcgat tattattatt
      301 atagaagccc ccatgaataa atcgctcatc attttcggca tcgtcaacat aacctcggac
      361 agtttctccg atggaggccg gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag
      421 ctgatggccg agggggcaga tgtgatcgac ctcggtccgg catccagcaa ccccgacgcc
      481 gcgcctgttt cgtccgacac agaaatcgag cgtatcgcgc cggtgctgga cgcgctcaag
      541 gcagatggca ttcccgtctc gctcgacagt tatcaacccg cgacgcaagc ctatgccttg
      601 tcgcgtggtg tggcctatct caatgatatt cgcggttttc cagacgctgc gttctatccg
      661 caattggcga aatcatctgc caaactcgtc gttatgcatt cggtgcaaga cgggcaggca
      721 gatcggcgcg aggcacccgc tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg
      781 cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc gccttgtcct tgatcccggc
      841 atggggtttt ttctgggggc tgctcccgaa acctcgctct cggtgctggc gcggttcgat
      901 gaattgcggc tgcgcttcga tttgccggtg cttctgtctg tttcgcgcaa atcctttctg
      961 cgcgcgctca caggccgtgg tccgggggat gtcggggccg cgacactcgc tgcagagctt
     1021 gccgccgccg caggtggagc tgacttcatc cgcacacacg agccgcgccc cttgcgcgac
     1081 gggctggcgg tattggcggc gctaaaagaa accgcaagaa ttcgttaact gcacattcgg
     1141 gatatttctc tatattcgcg cttcatcaga aaactgaagg aacctccatt gaatcgaact
     1201 aatatttttt ttggtgaatc gcattctgac tggttgcctg tcagaggcgg agaatctggt
     1261 gattttgttt ttcgacgtgg tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc
     1321 cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct
     1381 tgccccgagg tcatcaactg gcaggaggaa caggagggtg catgcttggt gataacggca
     1441 attccgggag taccggcggc tgatctgtct ggagcggatt tgctcaaagc gtggccgtca
     1501 atggggcagc aacttggcgc tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc
     1561 aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc
     1621 gacttcttac cggacgagga caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa
     1681 cgagagctac cggtgcggct cgaccaagag cgcaccgata tggttgtttg ccatggtgat
     1741 ccctgcatgc cgaacttcat ggtggaccct aaaactcttc aatgcacggg tctgatcgac
     1801 cttgggcggc tcggaacagc agatcgctat gccgatttgg cactcatgat tgctaacgcc
     1861 gaagagaact gggcagcgcc agatgaagca gagcgcgcct tcgctgtcct attcaatgta
     1921 ttggggatcg aagcccccga ccgcgaacgc cttgccttct atctgcgatt ggaccctctg
     1981 acttggggtt gatgttcatg ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg
     2041 ttctcattgc ggacaccttt tccagcctcg tttggaaagt ttcattgcca gacgggactc
     2101 ctgcaatcgt caagggattg aaacctatag aagacattgc tgatgaactg cgcggggccg
     2161 actatctggt atggcgcaat gggaggggag cagtccggtt gctcggtcgt gagaacaatc
     2221 tgatgttgct cgaatatgcc ggggagcgaa tgctctctca catcgttgcc gagcacggcg
     2281 actaccaggc gaccgaaatt gcagcggaac taatggcgaa gctgtatgcc gcatctgagg
     2341 aacccctgcc ttctgccctt ctcccgatcc gggatcgctt tgcagctttg tttcagcggg
     2401 cgcgcgatga tcaaaacgca ggttgtcaaa ctgactacgt ccacgcggcg attatagccg
     2461 atcaaatgat gagcaatgcc tcggaactgc gtgggctaca tggcgatctg catcatgaaa
     2521 acatcatgtt ctccagtcgc ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag
     2581 tgggctttgg cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg
     2641 atcctagacg cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc
     2701 gtcgcctgct cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg
     2761 gagaagagga gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt
     2821 catactagat atcaagggca ctgttgcaaa gttagcgatg aggcagcctt ttgtc
//
LOCUS       DAJVTY010000394         2431 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.guided.273, whole genome shotgun sequence.
ACCESSION   DAJVTY010000394 DAJVTY010000000
VERSION     DAJVTY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.guided.273"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..483)
                     /locus_tag="OQQ66_004257"
     CDS             complement(<1..483)
                     /locus_tag="OQQ66_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCS1906928.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRIRALLQIVGGDKLI
                     I"
     gene            641..1114
                     /locus_tag="OQQ66_004258"
     CDS             641..1114
                     /locus_tag="OQQ66_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA7"
                     /protein_id="HCS1906929.1"
                     /translation="MKISLISATSENGVIGNGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSRKGISSSNENVLVFPSIEIALQELSKITDHLYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDINFPKIPENFNLVFEQFFLSNINYTYQIWKKG"
     gene            1344..1691
                     /locus_tag="OQQ66_004259"
     CDS             1344..1691
                     /locus_tag="OQQ66_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCS1906930.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1685..>2431
                     /gene="folP"
                     /locus_tag="OQQ66_004260"
     CDS             1685..>2431
                     /gene="folP"
                     /locus_tag="OQQ66_004260"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1906931.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHGHCCKVSDEAAFC"
BASE COUNT          566 a          616 c          633 g          616 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc ctgatccgca tgcccgttcc
       61 atacagaagc tgggcgaaca aacgatgctc gccttccaga aaaccgagga tgcgaaccac
      121 ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg
      181 aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg ccgccaatgc
      241 ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc
      301 gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
      361 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
      421 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
      481 catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc
      541 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
      601 cagtcgccct aaaacaaagt tagccattac gggggttgaa ttgaaaattt cattgatttc
      661 tgcaacgtca gaaaatggcg taatcggtaa tggccctgat atcccatggt cagcaaaagg
      721 tgagcagtta ctctttaaag cgctcacata taatcagtgg ctccttgttg gaaggaaaac
      781 atttgactct atgggtgttc ttccaaatcg aaaatatgca gtagtgtcga ggaaaggaat
      841 ttcaagctca aatgaaaatg tattagtctt tccttcaata gaaatcgctt tgcaagaact
      901 atcgaaaatt acagatcatt tatatgtctc tggtggcggt caaatctaca atagtcttat
      961 tgaaaaagca gatataattc atttgtctac tgttcacgtt gaggttgaag gtgatatcaa
     1021 ttttcctaaa attccagaga atttcaattt ggtttttgag cagttttttt tgtctaatat
     1081 aaattacaca tatcagattt ggaaaaaagg ctaacaagtc gttccagcac cagtcgctgc
     1141 gctccttgga cagtttttaa gtcgcggttt tatggttttg ctgcgcaaaa gtattccata
     1201 aaaccacaac ttaaaaactg ccgctgaact cggcgttaga tgcactaagc acataattgc
     1261 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     1321 ctacacaaat tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg
     1381 gcgaagtaat cgcaacatcc gcattaaaat ctagcgaggg ctttactaag cttgcccctt
     1441 ccgccgttgt cataatcggt tatggcatcg cattttattt tctttctctg gttctgaaat
     1501 ccatccctgt cggtgttgct tatgcagtct ggtcgggact cggcgtcgtc ataattacag
     1561 ccattgcctg gttgcttcat gggcaaaagc ttgatgcgtg gggctttgta ggtatggggc
     1621 tcataattgc tgcctttttg ctcgcccgat ccccatcgtg gaagtcgctg cggaggccga
     1681 cgccatggtg acggtgttcg gcattctgaa tctcaccgag gactccttct tcgatgagag
     1741 ccggcggcta gaccccgccg gcgctgtcac cgcggcgatc gaaatgctgc gagtcggatc
     1801 agacgtcgtg gatgtcggac cggccgccag ccatccggac gcgaggcctg tatcgccggc
     1861 cgatgagatc agacgtattg cgccgctctt agacgccctg tccgatcaga tgcaccgtgt
     1921 ttcaatcgac agcttccaac cggaaaccca gcgctatgcg ctcaagcgcg gcgtgggcta
     1981 cctgaacgat atccaaggat ttcctgaccc tgcgctctat cccgatattg ctgaggcgga
     2041 ctgcaggctg gtggttatgc actcagcgca gcgggatggc atcgccaccc gcaccggtca
     2101 ccttcgaccc gaagacgcgc tcgacgagat tgtgcggttc ttcgaggcgc gggtttccgc
     2161 cttgcgacgg agcggggtcg ctgccgaccg gctcatcctc gatccgggga tgggattttt
     2221 cttgagcccc gcaccggaaa catcgctgca cgtgctgtcg aaccttcaaa agctgaagtc
     2281 ggcgttgggg cttccgctat tggtctcggt gtcgcggaaa tccttcttgg gcgccaccgt
     2341 tggccttcct gtaaaggatc tgggtccagc gagccttgcg gcggaacttc acgggcactg
     2401 ttgcaaagtt agcgatgagg cagccttttg t
//
LOCUS       DAJVTY010000395         1903 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.guided.288, whole genome shotgun sequence.
ACCESSION   DAJVTY010000395 DAJVTY010000000
VERSION     DAJVTY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.guided.288"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            <1..290
                     /locus_tag="OQQ66_004261"
     CDS             <1..290
                     /locus_tag="OQQ66_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1906932.1"
                     /translation="KFITTDYLQQCHMGQMVVTILSAVAQAERRRILERTNEGRQEAK
                     LKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            473..1333
                     /locus_tag="OQQ66_004262"
     CDS             473..1333
                     /locus_tag="OQQ66_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCS1906933.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1480..1761
                     /locus_tag="OQQ66_004263"
                     /pseudo
     CDS             1480..1761
                     /locus_tag="OQQ66_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          481 a          423 c          501 g          498 t
ORIGIN      
        1 ataagtttat caccaccgac tatttgcaac agtgccatat ggggcaaatg gtggtcacca
       61 tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct agaacgcacg aatgagggcc
      121 gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag gcgtaccgtg gacaggaacg
      181 tcgtgctgac gcttcatcag aagggcactg gtgcaacgga aattgctcat cagctcagta
      241 ttgcccgctc cacggtttat aaaattcttg aagacgaaag ggcctcgtga tacgcctatt
      301 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      361 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      421 catgagacaa taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat
      481 tcaacatttt cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      541 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      601 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      661 ttttccaatg atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga
      721 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      781 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      841 tgccataacc atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc
      901 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      961 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     1021 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1081 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1141 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1201 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1261 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1321 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1381 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1441 cccttaacgt gagttttcgt tccactgagc gtcagacccc ttatggcaga gcagggaaag
     1501 gaattgccgg gctatgtgca acgggaattt gaagaatttc tccaatgcgg gcggctggag
     1561 catggctttc tacgggttcg ctgcgagtct tgccacgccg agcacctggt cgctttcagc
     1621 tgtaagcgtc gcggtttctg cccgagctgt ggggcgcggc ggatggccga aagtgccgcc
     1681 ttgctggttg atgaagtact gcctgaacaa cccatgcgtc agtgggtgtt gagcttcccg
     1741 tttcagctgc gtttcctgtt tggggtcgtt tgcgggaagg ggcggaatcc tacgctaagg
     1801 ctttggccag cgatattctc cggtgagatt gatgtgttcc caggggatag gagaagtcgc
     1861 ttgatatggc actgttgcaa atagtcggtg gtgataaact tat
//
LOCUS       DAJVTY010000396         1306 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 403067
            SAMEA8900091-rid15856573.guided.308, whole genome shotgun sequence.
ACCESSION   DAJVTY010000396 DAJVTY010000000
VERSION     DAJVTY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900091
            Sequence Read Archive: ERR6005796
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478710.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,186
            Genes (coding)                    :: 3,861
            CDSs (with protein)               :: 3,861
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 179 of 325
            Pseudo Genes (incomplete)         :: 88 of 325
            Pseudo Genes (internal stop)      :: 97 of 325
            Pseudo Genes (multiple problems)  :: 36 of 325
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900091-rid15856573.guided.308"
                     /serovar="S. boydii 2"
                     /isolate="403067"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Kenya"
                     /collection_date="2010-02-12"
     gene            complement(<1..181)
                     /locus_tag="OQQ66_004264"
     CDS             complement(<1..181)
                     /locus_tag="OQQ66_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1906934.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKP"
     gene            442..900
                     /gene="gpt"
                     /locus_tag="OQQ66_004265"
     CDS             442..900
                     /gene="gpt"
                     /locus_tag="OQQ66_004265"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1906935.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            992..>1306
                     /locus_tag="OQQ66_004266"
     CDS             992..>1306
                     /locus_tag="OQQ66_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1906936.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQA"
BASE COUNT          329 a          319 c          326 g          332 t
ORIGIN      
        1 caggtttacg aatcaggata ttacctacct gatcgcgttc gacatggaaa cctttctctt
       61 ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg ataggacggg tgcggaatag
      121 aacagatttt ggcaaaaata tcccacagcg gctgtggaga taattgagac agttcagaca
      181 cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg gttagcagaa
      241 aatgttgtca acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
      301 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
      361 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
      421 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
      481 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
      541 gccgtaagcc gtggcggtct ggtaccgggt gcgttactgg cgcgtgaact gggtattcgt
      601 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgaga gcttaaagtg
      661 ctgaaacgcg cagaaggcga tggcgaaggc ttcatagtta ttgatgacct ggtggatacc
      721 ggtggtactg ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
      781 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatattcc gcaagatacc
      841 tggatcgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
      901 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggca ggttacaata
      961 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
     1021 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
     1081 cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat
     1141 gattaaccgt ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca
     1201 ggcacgtatt gtgatgagcg atgctgaacg taccgacgat gatttatacg atactgtgat
     1261 tggctaccgt ggtggcaact ggatttatga gtgggccacc caggcg
//
